LOCUS       DAOSIM010000001        31678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_1615_length_31678_cov_7.563482, whole genome shotgun sequence.
ACCESSION   DAOSIM010000001 DAOSIM010000000
VERSION     DAOSIM010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 31678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31678
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1615_length_31678_cov_7.563482"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PLR47_00005"
     CDS             complement(<1..354)
                     /locus_tag="PLR47_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11342.1"
                     /translation="MSKKKAETRRLAPAKINLFLRILRKRPDGYHDLASVMQQISLYD
                     EMTFSLRSKEIVISCPGADLPTDEKNLVYLAARKLFDHAGYSGGVAIELAKQIPLAAG
                     LGGGSSDAAATLLALN"
     gene            complement(335..730)
                     /locus_tag="PLR47_00010"
     CDS             complement(335..730)
                     /locus_tag="PLR47_00010"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOG11343.1"
                     /translation="MAEETKGEGFVIKDRRFSEEPDTQTKEEPASAKTEEPKETAGKT
                     SAEPADYPAVNFTNFVISLSTSALFHFGDFPEYEGGKAEKNLPAAKQTIDILDMLQKK
                     TAGNLDPNEAGLIEGVLYELKMRYVKEKG"
     gene            complement(841..3429)
                     /gene="clpB"
                     /locus_tag="PLR47_00015"
     CDS             complement(841..3429)
                     /gene="clpB"
                     /locus_tag="PLR47_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOG11344.1"
                     /translation="MRFDKFTLKVQEALQDAQTLAGTLGHQAIEPEHLLASLLQQQDG
                     IAGAILNKLNVSPRKVEQELHIDLKKQPGVEGAGAGQAYLGGRLNKLLDKAMTGALQL
                     NDEYVSAEHVLVAMSEEKEGKTGKILRQNGVTKDNILKVLVDIRGNQRVTDPNPEGKY
                     QALERYAKDFNELARTGKFDPVIGRDEEIRRIMQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVNGDVPENLKSKRVIGLDVGALIAGAKYRGEFEERLKAVLKEVISAEGEII
                     LFIDEIHTVVGAGASEGSMDASNMLKPALARGELRCVGATTLNEYRKYIEKDAALERR
                     FQPVLVKEPTVEDTIAILRGLKERYEVHHGVRIKDSAIVAAATLSHRYIADRFLPDKA
                     VDLIDEAASRLRIELDSMPSEIDAIDRRIIQLEIEKQSLKKDTDKAALERRDKIEKEL
                     AGLQSEMDRMKMHWSSEKDIIKKIQQIKSQMEALKTEEAGAQREGNLARAAEIRYGKM
                     VELSKALETDQAKLSDVQKNNRMLKEEVDAEDVAEVVSNWTGIPLSRMMESDVQKLIH
                     MDERLQMRVIGQDDGIGAVSAALRRARSGLQDPNRPIGSFIFLGPTGVGKTELARALA
                     EFMFDNEQAMIRIDMSEYMEKHAVARLIGAPPGYVGYDEGGYLTEAVRRKPYSVLLFD
                     EIEKAHPDVFNILLQILDDGRLTDGHGRTVDFKNTVVIMTSNVGSGWIQDPSLEEEEK
                     KNRSMEALRATFKPEFLNRIDDIIMFHGLTLEDIHRIVGIQLGLIGRRLAERKLSIEL
                     TEAAKKYIAEEGYSPVYGARPLKRALQKLVLDPLAMDLLAGKFSEGDHIVIGVSKGEI
                     TFSKKG"
     gene            complement(3443..4321)
                     /locus_tag="PLR47_00020"
     CDS             complement(3443..4321)
                     /locus_tag="PLR47_00020"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOG11345.1"
                     /translation="MNMKSVNKTMIETAIKLAKGVKAGCVLVCVDVRSELAELSEEER
                     KSVRFVLVLREDEELPEKLLPAAKKLELPDVNLTRVGKIKIAIAKGIVSGLFKKGDKV
                     VCLSGVPKFGYADSIFFIDVGREFEILTSDDISDVVEAVRPEVFNAVLNIACELAAQG
                     RENRKVGTIFVLGDDEKVMQLSRQMIINPFKGYSEEQRNILNPELEETIKELSAIDGA
                     FVIKTDGVIVTAGRHLNAALDSKDFPSGLGSRHIAAAGITNVTRAVAMVISQSSGNVS
                     VFKNGKLFVTIEKPVE"
     gene            4466..5311
                     /gene="rpoH"
                     /locus_tag="PLR47_00025"
     CDS             4466..5311
                     /gene="rpoH"
                     /locus_tag="PLR47_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HOG11346.1"
                     /translation="MKLPVVTGTLDLYIAEINRFPLLTAEEEFKLAMRLKKDNDMEAA
                     EKLIVSNLRFVVKIAHEYRSYGFKLSDLVQEGNIGLIHAVKKFDPEKGYRLISYAVWW
                     IRAYIQNYLIKSWSLVKIGTTQAQRKLFFKLSQARKQLETISKKNPEFSEIADSLGVK
                     GSEVAEMDLRMGARDLSLNELINDDGETSHLDFLTHDGDNQEVSLIKHEERSLVKRHI
                     AGALARLNERESYIIQHRVMADDPLTLQEIGDKYNITRERARQIEKQALKKVQLALPY
                     LKEDL"
     gene            complement(5316..5921)
                     /locus_tag="PLR47_00030"
     CDS             complement(5316..5921)
                     /locus_tag="PLR47_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOG11347.1"
                     /translation="MTTRSFNKRTGILMLAAAVLAAGVLYAPRYLAYSTGGARADAVV
                     VLLGPVFNDRDRYARDLMNRGMADYLVIPAYQKLFSADRGKLKAVFIPKDPDRLKKNP
                     DSAWPPFYEDTHLELIEAKRIMRQYGQKTAIFVSSPYHMRRIRIMVGREFGTSDGHCF
                     LPTPYEKAPVNVWELKASDWRKVGREYLKIVWFLVYSPWVR"
     gene            complement(5948..8572)
                     /gene="mutS"
                     /locus_tag="PLR47_00035"
     CDS             complement(5948..8572)
                     /gene="mutS"
                     /locus_tag="PLR47_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOG11348.1"
                     /translation="MALNATFNLTPAMKQYVEIKEKYPDCILFYRMGDFYEMFFEDAQ
                     RAAPLLEITLTSRNKSSQDAVPLCGVPYHAASAYITRLVEKGYKVAVCEQVEDPKNAK
                     GIVRREVVRVITPGLVLDEENLKASENNYLACLAAGPDALGLAFLDLSTGEFYISEFP
                     GRDALLAASGPLAFRELLVDRNFPDPALVKMLEARMPSVCVNTIDAAGFEPGQADEIL
                     EKELDAAVLEKSGIRGFRAAKNAAAGILRYVNATQRVRAGHISAIREYAPRNYMMLDE
                     AARRNLEIFATLQDGRSAGSLFHLINETLTPMGARRLRWWMGYPLTAPEKIRERLAAV
                     AEIRESHAARAQLRKILSGVYDLERLAGRIALMAATPRDLVALKTSLAVLPSLRSGLE
                     EFTAPALSALRKDMTDMSKTVQLLSRAIVADPPARAQDGGFIAAGFDEELDRLRFVSA
                     DGKQWIAALEARERQTTGIHSLKVGYNSVFGYYIEVTKANAALVPDSYVRKQTLVNAE
                     RYINQELKEFEQTVLHAEEKIRARESELFGALRESLLPDIPVIQYNAFCVAELDAISA
                     LAETAEKHSYVCPEISEDDVIDIRDGRHPVVEAALKKEGFVPNDTLLDLGQNRLLVIT
                     GPNMAGKSTYIRQVALIVILAQMGSFVPASSARIGVADKIFTRIGASDSLAKGQSTFM
                     VEMMETAQILTQATPRSLVILDEVGRGTSTFDGLSIAWAVAEHLHDLAGRGVRTLFAT
                     HYHQLTDLAASRQGAKNYNIAVKEWGEKIIFLRRIMEGGTSRSYGIEVARIASVPADV
                     IARAREILHNLEKGEFDEIGMPRIARGRNVRKPANPQLSLFAEGEDIVLEEIRRLDVS
                     SMTPLEALNILSELKSKL"
     gene            complement(8563..8997)
                     /locus_tag="PLR47_00040"
     CDS             complement(8563..8997)
                     /locus_tag="PLR47_00040"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOG11349.1"
                     /translation="MAAGQGSFITVLLIRWLVITAAIVLASSLLPGIHVASLATAFLA
                     AAILGVLNTFLRPVLLLLTLPLTILTLGIFAFVLNALMLILVAYFVPGLEIDGFFWAF
                     LGALIVSIASWLANRFIYQQQAAPRGKPDVIDLERGDDGKWR"
     gene            complement(9006..9854)
                     /locus_tag="PLR47_00045"
     CDS             complement(9006..9854)
                     /locus_tag="PLR47_00045"
                     /inference="COORDINATES: protein motif:HMM:NF018352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HOG11350.1"
                     /translation="MAYGRIFPVLLAFVLLILPIKAAGAEKPLWEVGVGGGFLMMPDY
                     RGSDKTRPYLLPFPYAVYRGGIFRLEDKRLTARLFKTDRVTLDASGYGAVPVKSDDNE
                     ARRGMEDLDPTFEFGPALRIKLLDSRENWHRLSLAFPVRAVFSTDFRSVRYEGWVFSP
                     RLNFEKGNVIPETGIYLGVSAGPMFGDRGCHQYFYNVEPPYAAADRPAYDAGGGYSGS
                     TLTVGLGKNYRSFHFHAFVSADFLQGAVFEDSPLVKTKTSWMSGFSVTWIFLKSKKTV
                     ADDTPY"
     gene            complement(9870..10322)
                     /locus_tag="PLR47_00050"
     CDS             complement(9870..10322)
                     /locus_tag="PLR47_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOG11351.1"
                     /translation="MDEKIREALREAYVGEAKAALRLSMFADKAEQEGYPQIARLFRV
                     IAFSEEIHGKRALRVLKEIKTTEENLAESFESEKTVAEIAYDRFVKMAEQAGDKQALL
                     HFSQSHFMEERQTVYHVCKVCGYVADGVLPEECPVCAAKKDQFTTFDR"
     gene            complement(10348..10506)
                     /locus_tag="PLR47_00055"
     CDS             complement(10348..10506)
                     /locus_tag="PLR47_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOG11352.1"
                     /translation="MKKYVCDVCGYVYDPDQGDEEGGIPPGTAFEKLPADWTCPVCGA
                     DKSEFKPE"
     gene            complement(10533..11744)
                     /locus_tag="PLR47_00060"
     CDS             complement(10533..11744)
                     /locus_tag="PLR47_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOG11353.1"
                     /translation="MTNIYSAIKVSDHVYWVGAIDWSIRDFHGYTTPRGSTYNAYLIL
                     ADKITLIDTVKKPFMEEMLARIASVIDPAKIDYIVSNHSEQDHCGCLSQVVGLVKPSK
                     VFASAVGVKNLNDILHNDYPIQAVREGETLSLGNMELTFMETRMIHWPDSMFTYLAAD
                     GLLFSQDAFGMHLATFERFADELPADMLEYEAATYYANIVLPYSPVVLKAMERVAATG
                     WAIKIIAPDHGPVWRKDLGFILERYQKWAEQKPSNKAVVLYATMWHSTEMMARAIAEA
                     LAQAGVKVKILSLNEAHRSEVIFELLDAGALIVGSSTLNNNMLPQMADGMTYIKGLKP
                     ANLAGAVFGSFGWSGEAVKDLEAVLREMKVDVVAETVSAKHVPGPDVLGKCHELGRAV
                     ASELAKRLSPI"
     gene            complement(11811..12425)
                     /locus_tag="PLR47_00065"
     CDS             complement(11811..12425)
                     /locus_tag="PLR47_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOG11354.1"
                     /translation="MKSKSQKVTLNLILTGVGGQGNVMASRTLAGMLVDAGYVATIGE
                     TFGMSQRGGSVMSHLRISPDAVRSPQIPRGAADVIVALEPLEALRVLTMYGNPEVSLI
                     ANSRSIDPVGVITGERDYPSEETIRSAFERLTARYWLFDATAAGLSLGNPVLSNIVMI
                     GALAGTGLLPIDRRMFAKEIRKTQSPEKCALNLKAFDAGVSMIK"
     gene            complement(12493..>12982)
                     /locus_tag="PLR47_00070"
     CDS             complement(12493..>12982)
                     /locus_tag="PLR47_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG11355.1"
                     /translation="DVTFIVLDNQGTAMTGFQPHPGIEVNALKESVPAVDIASICRAI
                     GARVALCDPFDLAATGAALTDFLAEKGSLKVLILRQACALSPEKKGKKTFEMKVDEDL
                     CLGDACGCNRLCTRIFRCPALVWDRKKKKAAIDEVVCAGCGVCASICPRKAIIKMQKT
                     VL"
     assembly_gap    12983..12992
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12993..14441)
                     /locus_tag="PLR47_00075"
     CDS             complement(<12993..14441)
                     /locus_tag="PLR47_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG11356.1"
                     /translation="MTKLLSPAKSKVLFLGNEAIARGALEAGVSVATGYPGTPSSEVI
                     ESLARVAQERNLYVEWSVNEKVALEVAAAASFAGLRSMAVMKQNGVNVASDFLLHLTA
                     SGTRGGIVLVSCEDPGALSSVNEGESRYFAGMLEMPLLEPADFQEAKDMTKWAFELSE
                     AIGNVVMLRSVTRMSHASGNVVCGKFPAKTRKAAFEYGGPIMDSQTGPMISTPVVAKH
                     LLRRQKLKAVCALFEKSPFNRYTGPARPDLLLITASACWLYAVEAVEMLGLQKRVGIL
                     KLGTTWPLPPRLVSGHLRKAGKILIVEEVMAFLEENVKVLAAGDPKLSGGKTFFGKYD
                     GTLPFAGELNPDIVADALAKIFRMKRGSLPSSYLEEARKTAALLPPREQTFCAGCPHR
                     ASFWSIHNAIALDGREGFVCGDIGCYSMAALTPANGYWTMPTLHSMGSGTGLASGFAK
                     LSPFGFQKPVLTVCGDSTFYHAVIPALINAQHN"
     gene            complement(14510..15640)
                     /locus_tag="PLR47_00080"
     CDS             complement(14510..15640)
                     /locus_tag="PLR47_00080"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOG11357.1"
                     /translation="MRKKRSSQSRLKKDVLEKSLCTGCGACVGLCPYQAVYKDRTVQL
                     FDCDLPDGKCRAFCPRTPSDLPLLRERLFDASDATDEIGPVKEYWLTRAKDEGLRSRA
                     QHGGTVTALLGLAMSEGWIDAAIVSGRLDGREPEGLLIDRAEELADYAQSRFWAAPTV
                     AAFHRAPESGAGRLGIVATPCQAIALAKIRTAKIDGYRKGGSLSLVIGLFCGWTLALD
                     RFQNLLEKYQLSASALTGMDIPAGKNVLELRTAGGSANVPMDEVDSCVREACRCCVDS
                     TAEFADLSVGAARFGANPEEMRGWNQVIVRSGAGQKLVSLARQRGVLEFREAPPPALV
                     ELKNAAAEKKKKALVRIIAKSRSPKNLLYLNPDDPVVRKYLK"
     gene            complement(15640..16419)
                     /locus_tag="PLR47_00085"
     CDS             complement(15640..16419)
                     /locus_tag="PLR47_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG11358.1"
                     /translation="MKNKIAVVAAASRGLGRAAARQLALEGATVVICSRRKKEITQAA
                     CEIQEETGSVVVPVRADVTKPKDVRDLVSQAGKRFGTVHVLVVNAGGPPAGEMLALTD
                     KDWQKAHELTLMSMVRLTRAVLPMMVRQQWGRIITINSIVAKQPVNELLLSAAIRPGI
                     GGLVKVLANQYAKYNITINTVCPGHIRTQRQEELARFRAEARRISIERYFAETAAAIP
                     AGRLGRPEEIGSVVAFLASEQAAYINGVNLLVDGSAAKGVH"
     gene            complement(16478..18496)
                     /locus_tag="PLR47_00090"
     CDS             complement(16478..18496)
                     /locus_tag="PLR47_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG11359.1"
                     /translation="MLDSLIRKYNSRKISTQYLLLLIPAILLLFLAFGFVAYTLGKPE
                     ALKTNYELAEVVAKKTNQALMRWLEEQIRTAQTIARDPRIIAVALSPADPQKRADAQK
                     YLSEMHARHPYYENIPLAAKLAPDRTVAVDVQGEKKIIGNGNFFIDTVGGKTIGKCGP
                     EFSYIKNVFAGKPYFISEVYPSILRGNPIFVVAAPIEHNGEIVGATIVAPRMDYFTEF
                     FVEKSVIGATGYLSMIDQDGMIISHPQKSLILNKKGPALVKPVMDKIAAGESYFLSDF
                     AGTTKAYYVEKLTTAQFHMEDSWYIIFCRNYDEVLSDIFAFLLKLLISMLLVAVAVAL
                     LVYFLTRSLVGKPMEKLTGAADSVAKGDLRVQIDPGSSRNEVGQLSRSINNMTRSIRE
                     QTIRIREAVDTLNATTASIVSASGRQEEAMKSLEGNVAQVGVSSRQISATSQELAGTM
                     ENVKNSAGEASRQANAGQKSLSGLQGAMQSLSAGTDSISEKLALMRQKAASIDSIIAT
                     ITKISDQTNLLSLNAAIEAEKAGEYGRGFSVVAREIGRLANETAISTLDISDIISEMQ
                     ASVADGVSEMDHFRQEVACGVRSAEDATMQLTEVIARVANLGPELEKVNEGMRFQSLG
                     SRQISEAMAELNAGAAQLTGSLSQFKAVTEQLNTAARGLSETVSRVVV"
     gene            18863..20170
                     /locus_tag="PLR47_00095"
     CDS             18863..20170
                     /locus_tag="PLR47_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOG11360.1"
                     /translation="MAGFTHQYREKLTTFEEAAKAVKDGDTLIHGVTIAEPPGLLAAI
                     AHRARLGSLNHLKVYTFNAQKHFAETLGSPDIADVIDSYSWFVSENSRNMVQVGLTQF
                     VPAYLHQVPKIVMETMDVDVAVATVAPMNEDGCFSFGPCNGYMSVMATLCKTLIVEVN
                     ENMPFVYGDALIHISDIEMIVENTVPLLELPPLQPNPLDPVIGKIVAEYIPDRAVIQL
                     GIGTLPNAIAPCLEGHRDLGIHTELLCPGMVDLIQKGVITGAHKNVHAGKHIFSLAYG
                     GKETFEYMNGNKSFENYPSSYVMNPGVIAQNHRMTAVNAILQIDLLGQCNAEFLEGRQ
                     YSGTGGQLDFVRGAYDSPEGKAILVLYSTAKNEKVSRIVSRFPAGTMVTTPRNDAHYV
                     ATEYGIVNLKGKSTRARALALISIAHPKFREGLMREAENMSLM"
     gene            20241..21791
                     /locus_tag="PLR47_00100"
     CDS             20241..21791
                     /locus_tag="PLR47_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG11361.1"
                     /translation="MTSEKLQKEMDQMRKTLNRLQESDAKHREAAEAFRKCEQEYREM
                     VELANSIILRMDTSGKILFVNDYALQFFGFSKEELIGKNLIGTIVPQMESSGRDLAAF
                     MASVCRKPGEHTTNENENMKKDGSRVWILWTNRPVLDEKGQIAEIISVGNDITEKKEA
                     EKILRNARDELEREVRERTKELKKLNEDLLFEVAERKISEEALKESEVRYRSIVENAF
                     EGIFQTTLEGKCTMANAALVSLLGYASAKEYMAATSRPENLYVEASDRQELIGLLKTD
                     GYVKGFETQFYRRDGGKISVSLNVRAMFDERGNFIYYQGTVIDISAEITLRRILDETT
                     GALSTAVEVRDPYTAGHQERVTRLAEAIAAEMNFSDEHISAVHTAGMLHDIGKIYVPA
                     EFLSRPGKITAEEYSILKRHAQEGFEILKNIEYKYPIAEIVYQHHERLDGSGYPRGLR
                     ADQILIEARIISVADVVEAMASPRPYRPALGIEKALNEIRQNRGVLYDENVVDTCLAL
                     FLHKEFYF"
     gene            complement(21849..22355)
                     /locus_tag="PLR47_00105"
     CDS             complement(21849..22355)
                     /locus_tag="PLR47_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11362.1"
                     /translation="MQVLKFKFGMSGERVDAFGMRFFGDFDSRTGHGGTFSEILSDPD
                     APAMTKHPRVTLWFMFEKIRPYDKVSRLLDQLSAGLKKKGYTIKVSSIDGLVDTTSPD
                     YADKPERRFPASCRMHGCNAAGGFSVTAEKTEAKLKFSSKEVAMIKEMTVKLGQTIYG
                     RSLKMHSL"
     gene            complement(22448..23674)
                     /locus_tag="PLR47_00110"
     CDS             complement(22448..23674)
                     /locus_tag="PLR47_00110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG11363.1"
                     /translation="MATAALHEKALISANDLSHTFGSGEVRLQVLRNVNVDFYPGEIA
                     IIMGPSGSGKTTLLTLAGALRSIQEGSIVIGGIELRGATPDRLLAVRHQIGFIFQAHN
                     LLESLNARENVQMGLVHRTDLTIAEIRSRSMAMLERVGLAGHEHKHPPQLSGGQRQRV
                     AVARALVREPSIIMADEPTAALDKTSGREIVELLQRLAREMRSAVLMVTHDNRILDLA
                     DRILTLEDGNVEENHRGLERVAGLLKEGINRIARYPAFFAGGPGRTPSSDDKAFADET
                     QQMLSRARADIHVLVARRLCVNLVFEAKMLAELSDAASLLEEGVRQFGDLFCARPAAA
                     SVDLVNSLDRGPMMESLRARHFERDEAASRQVKDYCFDISNLFARLIYFLHVLAGQLA
                     ELGREKKNRDRDKKTK"
     gene            complement(23690..24874)
                     /gene="devC"
                     /locus_tag="PLR47_00115"
     CDS             complement(23690..24874)
                     /gene="devC"
                     /locus_tag="PLR47_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease DevC"
                     /protein_id="HOG11364.1"
                     /translation="MKVFSRIQAWLAPGGISLAWRQLSYDKKRFATAIAGVSFGVIIM
                     LFQQGIYQAFQIMVIRPFVAMQGELALISPDYQYLMTPGHFPERRLYQTISDGDVETV
                     YPVSLSYQKWRNPETGGKIDAAMIGVYAGRNPFIIPEVASREDVLTSPENILFDDGSS
                     PDFGDVAGLFAKQGEVETEINDQRIRVSGIFSMGQTLASYGHVIVGMEAYQRLSGLPS
                     GTINLGMIRLKPGADPEAVAQRLQKIVPEDVLVLTRGQLIKREQVYWQNNTPVGFITI
                     AGMILAMFVGAVIVYQILYTDINDHLKEYATLTAIGLGPGYFVKFILTESLILLVIGF
                     IPGLLITALLFLLAESSAELPTRITLFDTALVFCMAAIMCLIAGFLATRRLRSVDPAD
                     IF"
     gene            complement(24871..25866)
                     /locus_tag="PLR47_00120"
     CDS             complement(24871..25866)
                     /locus_tag="PLR47_00120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOG11365.1"
                     /translation="MHNQYTIQASRLFVVVLILAGMVFPVRAAEQKTNISCLGQIIAG
                     ERTIVLSAPEGAIMDRLLVKRGDQVARGAELARLRDYDAQAAAVDCARRDIGLARAGL
                     TLVQKGERPEQVEAQQAVVAAREAALRMHKSKKERYQQLHAKSIVPDDAYEAVLYDFE
                     AAQADLLREKNVLEGMQSGHKEEIRQASAKVAVVEANYRRQMALLEAQRIRAPIAGRI
                     LSIRAYPGEAIGESGILDLADTGKMMILAEIYETDISRVRIGSRAQIRSTVFEGEIGG
                     QVVEIERKVEAGRIYALDPRRHADRRIVMVRIKPDKPERLAPFSNAQVTIILHTR"
     gene            complement(25922..28882)
                     /locus_tag="PLR47_00125"
     CDS             complement(25922..28882)
                     /locus_tag="PLR47_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG11366.1"
                     /translation="MADSSNRNRSSKTVPEKKTANSKTSKARSKPVLSPGGARVSPSN
                     DIYHLSKDILSNVGVAIYIVQRGWFVYVSSLYQTMTGYSEKELLHHHCLEFIHPEDRE
                     KTRENAIAALKRERTDPYEYRFIKKNGDVMWILERVASIPYEGTRAALGSFMDITERR
                     RMEMIIRQSEEKYRSILEGIEDGYAELDLKGNLVFFNEALCRIQGYPREELARENYRR
                     LMDEENAAKMFASYHRVFTTGQSEKDVQYEVIAKDGARKSIESSITPVKNDAGRIVAF
                     RGILRDRTGLKQAEEALRRSEERYRTILEESDNAYFEVDLAGNYTFVNDALSRLLGYS
                     KEELIGRTFRDQVNKEDTHLLYRAFGKIYTTGEPERGMVYKSTRKGGETRYSEVSGFP
                     LHNAKGEIVGFRGIGRDITLQRLLEEALRQSEERYRTILEEMDDAYFEVDLAGNYTFV
                     NDAISRLLGYPKEKLLGRTFRDQVDKKDTKILYNAFGKIYTTGKPQRGIFYKYARKDG
                     SARYAEISGFPLHNSKGEIVGFRGIGRDITERKKSEERIQYLATHDGLTGLPNRILFN
                     QLLQRALQSAKRYQRMFAMFFIDLDRFKMINDTLGHDAGDQLLREIAVRFKQKLRAVD
                     TVARLGGDEFVILIEELEDLSYVTTVARRILSAALQPMTIMGEECRVTASIGVSLFPK
                     DGEDEQTLMKNADVAMYFAKEDGKNNFKFYSHDIQSQSARRLSIETRLRFALEKNEFF
                     LQYQARLDIRTGKITGVEALLRWDNPDLGLLMPARFIPVAEDTGMIVPIGRWVLRTAC
                     FQNAAWQKMGLPPVCMSVNLSMRQLNDVHLIVDVERVLRESGLAPHLLELEITEHMIM
                     HNPARIIAVLNNIRKLGVRIAIDDFGTGYSSLAQIRHFPVDALKIDRSFMRNIMENPE
                     DIAVARAILSMGKTLSLTVVAEGVETEEQMAFLQKHACDEMQGFHFSAPVSPEQFADL
                     LRNHLPSSIKSCKE"
     gene            complement(29165..29241)
                     /locus_tag="PLR47_00130"
     tRNA            complement(29165..29241)
                     /locus_tag="PLR47_00130"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29205..29207),aa:Met,seq:cat)
     gene            complement(29338..30183)
                     /gene="speB"
                     /locus_tag="PLR47_00135"
     CDS             complement(29338..30183)
                     /gene="speB"
                     /locus_tag="PLR47_00135"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOG11367.1"
                     /translation="MNYGGIYPECSLRNAKFVVVPVPYDSTSTYQPGSRRGPAAILEA
                     SANMELYDEELKTETFLAGIHTALPVEVDARGPKTMIDAVRKRIRRIVAQEKIPVMLG
                     GEHSITLGAVRAVAEKYPNLTVLQFDAHADLRDRYQKTPYNHACVARRVAEFCDLVQV
                     GIRSMSREEGEFLPESSVKSYSADDVFKHPNWAETVAKSIKGDVYVTIDLDVFDPSVM
                     PATGTPEPGGLFWRDVLGLLRLVSRKCAVRGFDVVELAPLPGVVAPDFMAAKLIYRFM
                     GYLTT"
     gene            complement(30221..31057)
                     /locus_tag="PLR47_00140"
     CDS             complement(30221..31057)
                     /locus_tag="PLR47_00140"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOG11368.1"
                     /translation="MMKAVFLSDAHLKRSTDEPYGLLVRFLTDLGEGRVGPAAAGKEA
                     AGAGTTVDDLYILGDFFDFWFCRPDRIYPEFQPVIRQLVQLRQSGVRIHLFEGNHDFF
                     MREYFYDVLGMDVVEESASIDLGGRRVFAAHGDTLDRTNVFYLTLRKVLRSRTFYHLQ
                     RLIPSPILWAAAGLSSATSKTVTIEDGAVLAEKMYACAVERLFTNHDAVILGHCHRPL
                     LRCSNGEGGKKIFAALGDWIIHRSYLFYEEGKFFMMRYPAGQESAREGFVEKLDISVS
                     SC"
BASE COUNT         6659 a         9459 c         8702 g         6848 t
ORIGIN      
        1 attgagcgcc agcagggtgg ccgcggcatc cgaactgccc ccgcccagac cggcggccag
       61 gggaatctgc ttggcaagtt cgatggcgac gccgcccgaa tagccggcat ggtcgaaaag
      121 ctttcgggcc gcgaggtaaa ccaggttttt ttcatccgtg ggaagatcag cgccggggca
      181 ggaaataaca atttcttttg agcggaggga aaaggtcatt tcatcgtaaa gggagatctg
      241 ctgcatcacg gaggccagat catggtaacc gtccggccgt ttgcgcaaaa ttcgcagaaa
      301 aaggttgatt ttggcgggcg ccaaccttcg cgtttcagcc tttttctttg acatagcgca
      361 ttttcagctc gtagagcacg ccctcgatca ggccggcttc gttcgggtcc agatttccgg
      421 cggttttctt ctggagcata tccagaatat cgatggtctg cttggcggcc ggaagatttt
      481 tttcggcctt gcctccttcg tactccggaa aatcgccgaa atgaaaaaga gcggacgtgg
      541 aaagcgaaat gacaaagttc gtgaaattca cggcgggata atccgccggt tccgcggaag
      601 ttttgccggc cgtttctttc ggctcctccg tctttgcgga tgccggctcc tcttttgtct
      661 gtgtgtccgg ctcttccgaa aaacgcctgt cctttatgac aaatccttcg cctttggtct
      721 cttccgccat aaaatccccc ctgattgtgc agggaacggt cgcgaccgtt ccctaccctt
      781 taatccttca gtcttcagcc tttcttcagc cttcagcctt cagccttcgg ccttccgtcc
      841 ttaacctttc ttgctgaacg taatctcccc tttggaaacg ccgatgacga tgtggtcgcc
      901 ttcggagaat ttaccggcca gcaggtccat ggccagcgga tccagaacaa gtttctgcag
      961 cgcccgcttg agcggtctcg ccccgtaaac cgggctgtag ccctcttcgg ctatatattt
     1021 tttcgccgct tctgtcaatt cgatggagag cttgcgctcc gcgagccgcc tgccgatcag
     1081 gccgagctgg atgccgacga tccggtgaat gtcctcgagc gtcaggccgt ggaacatgat
     1141 gatgtcgtcg atgcggttta gaaactccgg tttaaaggtc gcgcgcaggg cttccatcga
     1201 acggtttttc ttttcttctt cctccagcga cggatcctga atccacccgc ttccgacgtt
     1261 ggaggtcatg atcacgaccg tgtttttgaa atccaccgtg cggccgtgcc cgtcggtaag
     1321 ccttccgtca tccagaatct gcaggagaat attgaacaca tccggatgcg ccttttcgat
     1381 ttcgtcaaac agcaggacgc tgtaaggctt gcgccggacg gcttcggtga ggtagccgcc
     1441 ttcatcatag cccacgtagc cgggcggagc gccgatgagc cgcgccaccg cgtgtttttc
     1501 catgtattcc gacatgtcga tgcggatcat cgcctgctcg ttgtcgaaca tgaactccgc
     1561 cagcgcccgc gcaagttccg tcttgccaac gccggtcggc cccaggaaga tgaacgaccc
     1621 gatcggacgg ttcggatcct gcaggccgga gcgcgcgcgc cgaagcgccg ccgagacagc
     1681 cccgatgccg tcgtcctgac cgatcacgcg catttgaagc cgctcgtcca tgtggatcag
     1741 cttctgcacg tcgctctcca tcatgcggga aagcggaatc cccgtccagt tggaaacgac
     1801 ttcggccacg tcctcggcgt ccacttcctc tttgagcatc cggttgtttt tctgtacgtc
     1861 cgaaagcttt gcctgatcgg tttccagcgc tttggaaagc tcgaccattt tgccgtagcg
     1921 gatttcggcc gcacgcgcca ggttgccttc gcgctgcgcg ccggcttcct ccgttttcag
     1981 cgcttccatt tggcttttga tctgctgaat ctttttaatg atgtcctttt ccgacgacca
     2041 gtgcatcttc atccggtcca tctcgctttg gagtccggcc agttcctttt caatcttgtc
     2101 gcgccgctcc agcgccgcct tgtccgtatc ctttttgagc gactgctttt cgatttcgag
     2161 ctgaatgatt cggcggtcga tcgcgtcgat ttcggacggc atcgaatcca gttcgatccg
     2221 cagccgggac gcggcttcgt cgatcaggtc caccgccttg tccggcagga accggtcggc
     2281 gatatagcgg tgagacaggg tagcggcggc cacaatcgcc gaatccttga ttcgcacgcc
     2341 gtgatgcact tcgtagcgct ccttgaggcc gcgcagaatg gcgatggtat cctccaccgt
     2401 cggctccttg actaaaaccg gctgaaagcg ccgctcgagc gccgcgtcct tttcgatgta
     2461 cttgcggtat tcattgagcg tcgttgcgcc gacgcagcgc agctcgccgc gcgccagggc
     2521 gggtttgagc atgttggagg cgtccatcga tccttccgag gctcccgcgc cgaccaccgt
     2581 atggatttca tcgataaaaa ggatgatctc gccttcggcg ctgatgactt ccttcaaaac
     2641 cgccttgagc ctttcttcaa attcaccgcg gtatttcgcg cccgcgatca aagcgccgac
     2701 atccagaccg atcacgcgct tgcttttcag attttccggc acgtcgccgt tgacgatgcg
     2761 ctgggcgagg ccttccacaa tggccgtctt gccgacgccc ggctctccga tcaagaccgg
     2821 attattcttg gtgcgccttg agagcacctg catgatgcgg cggatttcct catcccggcc
     2881 gatgaccggg tcaaactttc ccgtacgggc cagctcattg aagtctttgg cgtagcgctc
     2941 cagggcctga tacttgcctt ccggattggg gtccgtcacg cgctgattgc cgcggatgtc
     3001 cacgagcacc tttaagatat tgtctttcgt gacgccgttt tgccgcagaa ttttacccgt
     3061 cttcccttcc ttttcctcgg acatggccac cagcacatgc tcggcgctga catattcgtc
     3121 gttgagctgg agcgcgccgg tcattgcctt atccaacagc ttgttgagcc tgccgcccag
     3181 gtaagcctga ccggcgcccg cgccttcgac gccgggctgc tttttcagat caatatggag
     3241 ttcctgctcc acctttcggg gagaaacgtt taatttattc agaatggcgc cggcaatccc
     3301 gtcttgctgc tgcagcagcg aagcgagcag atgctccggc tcgatggcct ggtggccgag
     3361 cgttccggcc agcgtctgcg catcctgaag cgcctcctgc actttcaagg taaatttatc
     3421 aaatctcatg gtcttcctct ttctactcca cgggtttttc aatcgtcacg aaaagctttc
     3481 cgtttttaaa gacgctgacg tttcccgaag actgggagat caccatcgcc accgcgcggg
     3541 tcacattggt gatgccggcc gctgcgatat ggcggctccc caggcccgaa gggaaatctt
     3601 tgctgtccag cgccgcgttc aaatggcgtc ccgccgtaac gatcacgccg tctgtcttga
     3661 tcacgaacgc gccgtcgatg gcggaaagct ccttgatggt ttcctccagc tccggattca
     3721 gaatgttgcg ctgctcctcg gaatatccct tgaacggatt gatgatcatc tgccgggaaa
     3781 gctgcatcac tttctcatcg tcccccagaa cgaaaatcgt tccgaccttc cggttttcgc
     3841 gcccctgggc cgccagttcg caggcgatgt tcaaaacggc gttgaaaacc tcgggccgca
     3901 cggcttccac gacatcgctg atgtcgtcgg atgtcaggat ttcgaactcg cggccgacgt
     3961 caatgaaaaa gatgctgtcc gcatacccga atttcggcac gccggacagg cagacgacct
     4021 tgtcgccctt tttaaaaagg ccggagacga tgcccttggc aatggcgatc ttgattttcc
     4081 cgacgcgggt cagattgacg tcgggaagct ccagtttctt tgccgccggc agcagcttct
     4141 ccggcagctc ctcgtcctcc cgcagaacga gaacgaaccg gactgatttt ctctcttcct
     4201 cggaaagctc ggccagttcg cttcggacat ccacgcagac cagaacgcag ccggccttga
     4261 cgcctttcgc gagcttgatg gccgtttcga tcatcgtctt gttcacggat ttcatattca
     4321 ttcccggttc ttttttgagc ctaaaataag catttgaagc cagcctgtca agccgcggac
     4381 ggccgccggg aaatatttgc ttgaccgctc attcactctt cggtatctac cagatcaggt
     4441 tttttggact gaggaggccc gcccgttgaa gttacccgtc gtcaccggca ctctggactt
     4501 atatatcgcg gagatcaacc gctttcccct tttgaccgcc gaggaagaat tcaaactggc
     4561 catgcggctg aaaaaggaca acgacatgga agcggcggaa aaactgatcg tttccaattt
     4621 gcggtttgtc gtgaaaatcg cccatgaata ccggagttac ggcttcaagc tgtccgatct
     4681 cgttcaggaa ggcaacatcg gactgattca cgccgtcaag aaattcgacc cggagaaagg
     4741 ctaccgcctg atttcctacg cggtctggtg gatccgcgcc tatattcaga actatttgat
     4801 caaatcctgg agcctcgtca agatcggaac cacacaggcg cagcgcaagc ttttcttcaa
     4861 actgagccag gcccggaaac agctggaaac gatctcgaag aagaatcccg aattcagcga
     4921 gatcgccgac tcgctgggcg tcaagggctc cgaggtcgcg gaaatggatc tgcggatggg
     4981 cgcgcgcgac ctgtcgctca acgaactcat caacgacgac ggcgaaacgt cccacctgga
     5041 ttttctgacc catgacgggg acaaccagga agtctccctg atcaagcatg aagagagaag
     5101 cctggtcaaa cggcacatcg ccggcgccct ggccaggcta aacgaaaggg aaagctacat
     5161 cattcagcac cgcgtcatgg ccgatgatcc gctgaccctg caggaaatcg gcgacaaata
     5221 caacatcacc cgcgagcgcg cccgccagat tgaaaagcag gcgctcaaaa aggttcagct
     5281 ggccctgccc tatctcaaag aagacctgta agccgtcagc ggacccacgg ggaataaacg
     5341 agaaaccata cgatcttcag gtattccctg cccacttttc tccagtccga ggccttaagc
     5401 tcccagacat tcaccggggc tttttcgtag ggcgtcggca aaaagcaatg tccgtcggac
     5461 gttccgaatt ccctgccgac cataatgcgg atgcgccgca tgtggtaggg agagctcacg
     5521 aagatcgccg ttttctgccc gtattgacgc atgattcttt tcgcctcgat gagctccagg
     5581 tgagtgtctt cgtaaaatgg cggccaggcg gagtcggggt ttttcttgag gcggtccggg
     5641 tcttttggaa tgaaaacggc cttcagtttt ccccggtcag cgctgaagag cttctgatag
     5701 gcgggaatca cgagatagtc cgccattccc cggttcatca aatccctggc gtagcggtcc
     5761 cggtcgttga aaacgggccc cagcagaacc acgacggcat ccgccctggc gccgccggtc
     5821 gaatacgcca gataccgggg cgcgtaaagc acccctgccg cgagaacggc cgccgccagc
     5881 atcagtatgc cggttcgctt attaaaggac cgcgtggtca tgggccgatt tctttttgtt
     5941 ttcccggtca taatttgctt ttcagctcgc tgaggatgtt gagcgcctcc agcggcgtca
     6001 tcgaagaaac gtccagccgg cggatttctt caaggacgat gtcctcgccc tccgcgaaca
     6061 gactcaactg cgggttggcc ggctttcgga cattccggcc gcgggcgatc cgcggcatgc
     6121 cgatttcgtc gaattccccc ttctccagat tgtgcaggat ttccctggcc cgggcaatga
     6181 catccgccgg gacggaggcg attctcgcca cttcgatgcc gtagctgcgg ctcgtccccc
     6241 cctccatgat ccggcgcagg aaaatgattt tttcccccca ttccttgacg gcaatgttgt
     6301 agtttttcgc gccctgcctg gacgcggcca ggtcggtcaa ctggtggtag tgcgtggcaa
     6361 acagcgtgcg cacgccccgc ccggcgagat cgtgaagatg ctcggccacc gcccaggcga
     6421 tgctgaggcc gtcaaacgtg gatgtgcccc ggcccacctc gtccagaatg accaggctgc
     6481 ggggcgttgc ctgagtgaga atctgagccg tttccatcat ctccaccata aacgtgctct
     6541 ggcctttggc caggctgtcc gaggcgccga tgcgcgtgaa gattttgtcg gccacgccga
     6601 tgcgggcgct ggaggcggga acgaaactgc ccatttgcgc cagaatcacg atcagggcca
     6661 cctggcggat atacgtggac tttcccgcca tgttgggtcc ggtaatgacc agcagacggt
     6721 tttggccgag atcgagcagc gtgtcattgg gcacgaagcc ttcctttttg agggctgctt
     6781 cgaccaccgg atggcggccg tcccgaatgt cgatcacgtc gtcttcgctg atttccgggc
     6841 agacatagga atgcttctct gccgtttccg caagagccga gatcgcatcc agttcggcca
     6901 cgcaaaaggc gttgtactga atgacgggaa tgtccggcag caggctttcg cgaagcgcgc
     6961 cgaagagttc actttcccgc gcgcggattt tttcctcggc gtggagcacc gtctgttcga
     7021 actctttcaa ttcctggttg atgtagcgct ccgcgttgac cagcgtctgc ttgcgcacgt
     7081 aggagtccgg caccagcgcg gcgttggcct tggtcacttc aatatagtag ccgaaaacgc
     7141 tgttgtagcc gactttgagc gaatggatgc ccgtggtctg acgctcccgg gcttcgagcg
     7201 ccgcgatcca ctgcttgccg tcggcgctga caaagcgcag gcgatccagc tcctcatcaa
     7261 agcccgcggc gataaatccc ccgtcctgcg cgcgggcggg aggatcggcg acgatcgcgc
     7321 gggaaaggag ttgaaccgtc ttggacatgt ccgtcatgtc tttgcgcaag gcactgagag
     7381 cgggcgcggt aaattcttcc agcccggatc gcaggctggg caaaacggca agagacgttt
     7441 tcagcgcgac gaggtcgcgc ggcgtggccg ccatgagcgc gatgcgtccg gccagtcttt
     7501 ccaggtcata gacgccggaa agaattttgc gaagctgtgc gcgcgccgcg tggctttccc
     7561 tgatttccgc cacggccgcc agccgctcgc ggattttttc cggcgccgta agcggatagc
     7621 ccatccacca ccgcaggcgc cgggcgccca tcggcgtcag ggtttcgttg atgaggtgga
     7681 aaagggatcc cgcgcttctt ccgtcctgaa gcgtggcgaa gatttccagg tttcggcggg
     7741 cggcctcatc gagcatcatg taattccggg gcgcgtattc ccgaatggcg gaaatatggc
     7801 cggcgcgaac cctctgcgtg gcgttcacgt agcgcagaat tccggcggcg gcgtttttcg
     7861 ccgcgcgaaa gccccggatg cctgattttt ccagcaccgc cgcatcgagc tccttctcca
     7921 gaatctcatc cgcctgtccg ggctcgaaac cggccgcatc gatcgtgttg acgcaaacgg
     7981 acggcattcg ggcctccagc attttgacga gcgccggatc gggaaaattt ctgtccacca
     8041 gcagctcccg aaaggccagc ggccccgagg cggcgagaag cgcgtcgcgt cccggaaatt
     8101 cggagatgta aaactcgccg gtggacaaat ccagaaacgc caggcccagc gcgtcggggc
     8161 cggcggccag gcaggccaga tagttgtttt cggaggcttt caggttttcc tcgtccagaa
     8221 ccagccccgg cgtgatcacg cggacgactt cgcgccggac gatgcctttg gcgtttttcg
     8281 ggtcctccac ctgctcgcag acggccacct tgtatccttt ttccacaagc cttgtgatgt
     8341 aggcggaagc ggcgtgatag ggaactccgc aaagcgggac cgcgtcttgg ctggatttat
     8401 tgcgggaggt gagcgtgatt tccagaagcg gcgcggccct ctgcgcgtct tcgaaaaaca
     8461 tctcgtagaa atcgcccatg cggtaaaaca aaatgcagtc cggatatttt tccttgatct
     8521 cgacgtactg cttcatcgcc ggggtcagat tgaacgtcgc gttcagcgcc atttcccgtc
     8581 atccccccgc tccagatcaa tcacgtcggg cttcccgcga ggagcggcct gttgctgata
     8641 gataaaacgg ttggcgagcc aactggcgat gctgacgatc agggcgccga gaaacgccca
     8701 gaaaaaaccg tcaatttcca gtcccgggac gaaataagcc accagaatca gcatgagagc
     8761 gttgagcaca aaagcaaaga tgcccagcgt cagaatggtc agcggcagcg tcagaaggag
     8821 caggacgggg cgaagaaaag tattcagaac gcccaggatc gcggcggcga gaaaggccgt
     8881 cgccagagac gccacgtgaa tgccgggaag gaggctcgac gcaagcacaa tggccgcggt
     8941 aatgaccagc caccgtatga gaagaaccgt tatgaatgat ccctggcccg ctgccatatt
     9001 cctcctcagt aaggcgtatc gtccgccacg gtttttttcg atttcagaaa gatccacgtg
     9061 acgctgaacc cgctcatcca ggatgttttc gtcttgacca gcgggctgtc ctcaaaaacg
     9121 gcgccctgca gaaaatcggc gctgacgaag gcgtgaaaat gaaaggacct gtaattcttc
     9181 cccagcccga cggtcagggt ggagccgctg tagcctccgc ccgcatcgta ggccgggcgg
     9241 tcagccgcgg cgtaaggggg ttccacgttg taaaaatatt gatggcagcc tcggtcgccg
     9301 aacatcggcc cggccgaaac tcccaggtag atgcccgttt cgggaatcac attgcctttt
     9361 tcaaagttca accgggggga aaagacccat ccctcgtagc gcacggagcg aaagtccgtc
     9421 gaaaaaaccg cccggacggg aaaggccagg ctcaggcgat gccagttttc ccgggagtcc
     9481 aaaagcttta tgcgcagcgc cgggccgaat tcaaacgtgg gatccaggtc ctccatgccg
     9541 cggcgcgctt cattgtcgtc gctcttcacg ggcacggccc cgtagccgct cgcatcgagg
     9601 gttacgcggt cggttttgaa aagcctggcg gtcagccttt tgtcctccag gcgaaaaatg
     9661 ccgccccggt aaaccgcata gggaaacggc agcaggtacg gccgcgtctt gtccgatccc
     9721 cggtaatccg gcatcatcag aaaaccgccg ccgactccca cttcccacag aggcttctcc
     9781 gcgccggcag ccttgatcgg caggatcagc aaaacaaaag ccagcagcac tgggaaaatt
     9841 cgaccatacg ccatcacgaa cgcccaatcc tagcggtcaa aagtcgtaaa ctggtctttt
     9901 ttcgccgcgc aaaccggaca ttcctccggc aatacgccgt ccgccacgta gccgcagacc
     9961 ttgcagacat gataaacggt ttggcgctct tccatgaagt ggctctggct gaagtgcaga
    10021 agggcctgct tgtcgcccgc ctgctccgcc atcttcacga agcggtcata ggcgatttcc
    10081 gccaccgttt tttccgactc gaagctttcc gcgaggtttt cttccgtggt tttaatttcc
    10141 ttgagcacgc gaagcgcccg cttgccgtgg atctcttccg aaaaagcaat cacgcgaaaa
    10201 aggcgcgcga tctgcggata accttcctgc tcggctttgt ccgcgaacat gctgagccgc
    10261 agggcggcct tcgcttcgcc gacgtaagcc tcgcgaagag cttcccgaat tttttcatcc
    10321 atcgcttacc cctttctgaa aaaacgccta ttccggcttg aactcgcttt tatcggcgcc
    10381 gcaaaccgga caggtccaat cggcgggaag cttctcaaac gccgttccgg gggggattcc
    10441 gccttcttcg tctccctggt ccgggtcata aacataaccg catacatcgc atacatactt
    10501 tttcatggtt cgcctccttg agtggtctta tttcatatcg gggacaatct tttggccagt
    10561 tccgacgcaa cggccctccc cagttcatgg catttcccga gcacatccgg acccggcaca
    10621 tgttttgccg aaaccgtttc ggccaccacg tccaccttca tttcccgaag aaccgcttcc
    10681 agatctttca ccgcctcgcc gctccagccg aacgagccga acaccgcgcc tgccagattg
    10741 gcaggcttga gccctttgat ataggtcatc ccgtcggcca tctgcggcag catgttattg
    10801 ttgagcgtgg aagaaccgac gatcagcgcc cccgcgtcca gcagctcgaa gatcacttcg
    10861 ctgcggtgcg cttcgttgag ggaaagaatt ttgaccttta cgcccgcctg cgccagagcc
    10921 tccgcaatgg ccctggccat catttcggtg gaatgccaca tggttgcgta aagcaccacc
    10981 gccttgttgg acggcttctg ctcggcccat ttctgatacc gctccaggat gaagcccagg
    11041 tctttgcgcc ataccggccc gtggtccggc gcgatgattt tgatcgccca gcccgttgcc
    11101 gccacccttt ccatggcttt gagaaccacc ggcgaatagg gcagaacaat gttggcgtag
    11161 tatgtggcgg cttcgtattc cagcatgtcg gccggcagtt cgtcggcaaa gcgctcgaac
    11221 gtcgccaggt gcatgccgaa ggcgtcctgc gaaaacagca ggccgtccgc ggccagatac
    11281 gtaaacatgg aatccggcca gtggatcatt ctggtttcca tgaaggtcag ctccatgttg
    11341 cccagggaaa gcgtctcccc ttcccggacc gcctgaatcg gataatcatt gtgaagaatg
    11401 tcgttgaggt ttttcacccc gacggccgag gcaaacactt tggagggctt gaccagcccg
    11461 accacctgcg agaggcatcc gcaatggtcc tgttcggagt ggttggaaac aatgtagtcg
    11521 attttggcag gatcgattac ggatgcgatc cgcgccagca tttcctccat gaagggtttt
    11581 ttgaccgtgt cgatgagcgt gattttgtcc gccaggatca ggtaggcgtt gtacgtgctt
    11641 ccgcggggcg tggtgtagcc gtgaaaatcg cggatcgacc agtcgatggc gcccacccag
    11701 tacacatggt cgcttacttt tatcgcggaa taaatattgg tcatgaagtt tcacctcggg
    11761 aaatgatttg tgaatgagta atataaggtt taccgccgga tcgtcaactt ttatttaatc
    11821 atggagacgc ccgcgtcaaa agccttcaga tttaaagcgc atttttccgg cgactgggtt
    11881 ttgcgaatct cttttgcgaa catccgccgg tcgatgggca aaaggcccgt cccggccagc
    11941 gcgccgatca tgacgatatt gctcagcacc ggattgccca gggaaaggcc cgcggccgtc
    12001 gcgtcaaaga gccagtaccg ggccgtcagc cgctcaaagg cggaacggat cgtttcttca
    12061 gaggggtaat ctcgctcgcc cgtaatcact ccgacgggat caatcgaacg ggaattggca
    12121 ataagcgaga cctcgggatt gccgtacatc gtcaaaacgc gaagggcctc cagcggctcc
    12181 agcgccacaa tgacatcggc ggcgcctcgg ggaatctgcg gacttctcac cgcgtcgggg
    12241 gaaatgcgca gatgactcat caccgaccct ccgcgctgcg acatgccgaa cgtttcgccg
    12301 atggttgcga catagcccgc gtccacgagc atgcccgcca gtgtgcgcga cgccatgacg
    12361 ttgccctgcc cgcccacgcc cgtgagaatc aggttgaggg tgactttttg agattttgat
    12421 ttcatgggct ttatccccgt aaaaaacgtt ttcaataacc cgactacata acaacctgaa
    12481 ggcggcgcga ggtcacaaga ccgttttctg catttttatg atcgctttgc gcggacaaat
    12541 cgaggcgcac acgccgcagc cggcgcagac cacctcgtca atggccgcct ttttcttttt
    12601 ccggtcccag acgagggccg gacagcggaa aatcctcgtg cacaggcgat tgcagccgca
    12661 ggcatccccc aggcacagat cttcgtccac cttcatctcg aacgtttttt tacccttttt
    12721 ttcggggctg agcgcgcagg cctggcgcag aatcagcacc ttgagcgaac ctttttcggc
    12781 cagaaaatcc gtgagcgccg ccccggttgc cgcaaggtca aaggggtcgc agagcgccac
    12841 tctcgcgccg atcgcccggc agatggaagc gatatcgacg gccggaacac tttccttgag
    12901 cgcgttcacc tcaatgcccg gatgcggctg aaaccccgtc atggccgtcc cctgattatc
    12961 cagcacaatg aacgtgacgt ccnnnnnnnn nngttgtgct gcgcgttgat gagcgccggg
    13021 atcaccgcgt gataaaatgt ggaatcaccg cagaccgtca gaacgggctt ttgaaatccg
    13081 aacggcgaga gctttgcaaa accgctggca agacccgttc cggaccccat ggaatgaagc
    13141 gtcggcatgg tccagtaacc gttggccggc gtgagggcgg ccatggaata gcagccgatg
    13201 tcgccgcaga caaacccctc gcggccgtcc agtgcgatgg cgttgtgaat gctccagaag
    13261 gatgcgcggt gcggacagcc ggcgcaaaac gtctgctccc gggggggcag cagcgccgcc
    13321 gttttgcgcg cctcctccag ataggaagag ggcagcgaac cgcgcttcat cctgaaaatc
    13381 ttcgccaggg cgtccgccac gatatcgggg ttgagctccc cggcaaacgg aagcgttccg
    13441 tcatatttac caaaaaacgt tttgccgccc gaaagcttcg ggtcgccggc ggcgaggacc
    13501 ttgacgtttt cttccagaaa cgccatcacc tcttccacga tcaatatctt tccggctttc
    13561 cgcaaatgac cggacaccag gcgcggcggc aggggccagg tggttccaag cttcaaaatg
    13621 cccaccctct tttgcagtcc cagcatttcg accgcctcca cggcataaag ccagcaggcg
    13681 ctggccgtga tgaggagcag atcgggccgc gccggacccg tatagcggtt gaacggactt
    13741 ttttcaaaca gggcgcaaac ggccttgagc ttttgccggc ggagcagatg cttggcgacc
    13801 accggagtgc tgatcatcgg accggtttgc gaatccataa tcggcccgcc gtattcaaac
    13861 gccgcctttc gggtttttgc cgggaatttt ccgcaaacca cgtttccgct ggcgtgcgac
    13921 atacgcgtga cggagcgcag catcacgacg ttgccgatcg cttcggaaag ctcaaaagcc
    13981 catttcgtca tgtccttggc ttcctgaaaa tccgccggtt ccaaaagcgg catctccagc
    14041 atgccggcaa aatagcgcga ctccccttcg ttgacgctgg agagcgcccc cggatcttcg
    14101 caggaaacca gcacgatgcc gccgcgggtt cccgaggccg tcaggtgcag cagaaagtcc
    14161 gacgccacgt tcacgccgtt ctgtttcatg accgccatcg aacgcaggcc cgcaaaggaa
    14221 gcggccgccg ccacctctag cgcgaccttc tcgttgaccg accactccac gtaaagattc
    14281 cgctcttgcg ccacacgcgc aagcgactcg atcacctcgg aagaaggcgt gccggggtag
    14341 ccggtcgcca ccgaaacgcc cgcttccagc gctccgcggg caatggcttc gtttcccaga
    14401 aacaaaacct ttgatttcgc cggtgagagt aattttgtca tatgcatctc gtctctcgtc
    14461 actcgcagaa aacggtttga accacccctg cgggcggcgc gactgttccc tactttaaat
    14521 attttcttac caccggatcg tcggggttca aatacagcag atttttgggc gaccggcttt
    14581 tggcgataat gcggaccagc gcctttttct ttttttccgc cgccgcgttt ttcagttcca
    14641 caagcgccgg cggcggcgcc tctcgaaatt ccagaacgcc ccgttggcgc gccagtgaaa
    14701 ccagtttttg tcccgcaccg ctgcggacaa tcacctgatt ccagccgcgc atttcctccg
    14761 gattcgcgcc gaagcgggcg gcgcccacgg acaggtcggc aaactccgcc gtggaatcga
    14821 cgcagcagcg gcaggcttca cgcacacagg aatccacctc gtccatcggc acatttgccg
    14881 agcccccggc cgtgcggagt tcgagaacat ttttccccgc gggaatatcc atgccggtca
    14941 gggctgacgc ggaaagttga tatttttcca gaagattctg aaaacgatcg agggcgagcg
    15001 tccagccgca aaacaaaccg atcaccagcg aaagcgaccc gccctttcgg tatccgtcga
    15061 ttttcgccgt cctgattttg gccagcgcga tcgcctggca gggcgtggcc acgatgccaa
    15121 gtctgcccgc gccgctttcc ggcgcgcggt gaaacgcggc gaccgtcggc gccgcccaaa
    15181 accggctttg agcataatcc gccagctctt ccgcgcggtc gatcagaagg ccttcgggtt
    15241 cgcgtccatc cagacggccg gaaacgatcg ccgcgtcgat ccatccttcg gacatcgcca
    15301 gcccgaggag cgccgtcacg gttccgccgt gctgcgcacg ggaccgcagt ccctcgtcct
    15361 tcgctcgggt gagccaatac tccttcaccg ggccgatttc gtcggtcgcg tctgaagcgt
    15421 caaaaagtct ttcccgaaga agcggcagat cggaaggtgt tcgggggcaa aaggcgcgac
    15481 atttgccgtc gggcaggtca cagtcaaaaa gctgaacggt tctgtctttg taaaccgcct
    15541 gataagggca cagccccacg cacgcaccgc agccggtgca gaggcttttt tccagaacgt
    15601 cttttttcag cctggactga ctgcttcgtt tttttctcat caatgaacgc ccttggccgc
    15661 gcttccgtcc accagcagat tcactccgtt gatgtaggcg gcctgttccg acgcgagaaa
    15721 cgccacgacg cttccgattt cttccggccg gcccagtctt ccggcgggga tggcggcggc
    15781 cgtttcggca aaataccgct cgatggagat ccgccgtgct tcggctcgga aacgggccag
    15841 ctcctcctgc cgttgggttc gaatgtggcc ggggcacacg gtattgatcg taatgttgta
    15901 ttttgcatac tggttggcca gcaccttgac aaggccgccg atgccggggc gtatcgcggc
    15961 ggagagcagc agttcattga cgggctgctt ggcgacaatc gagttgatgg taataatcct
    16021 gccccactgc tggcgcacca tcatgggcag cacggcgcgg gtcagacgca ccatgctcat
    16081 cagcgtcagc tcatgcgcct tttgccagtc tttgtcggtc agcgcaagca tctcgcctgc
    16141 cggaggcccg ccggcgttta cgaccagcac atgcaccgtc ccaaagcgtt ttccggcttg
    16201 cgacaccaga tcccgaacgt ccttcggttt ggtgacatcg gcccgaacgg gaacgacgac
    16261 cgaaccggtt tcttcctgaa tctcgcaggc cgcttgagtg atttcctttt tgcgccgcga
    16321 gcagataacc accgtcgctc cttccagcgc aagctggcgc gccgccgccc tgcccagtcc
    16381 ccggcttgcc gcggcgacga cggctatttt gtttttcaat ccaaaatcca taaaccctcc
    16441 gggttttctt catgcgaagc acgaacgcaa aagcgcatta cacgacgacg cgggagacgg
    16501 tctcgctcag tcctctggcg gcggtattca actgctccgt gacggcttta aactgggaca
    16561 gagaaccggt caactgcgcc gcgcctgcgt tcagttcggc catggcttcg ctgatctggc
    16621 gggagcccag ggactggaag cgcatgcctt cattgacctt ttctaattcc gggccgagat
    16681 tggccacacg ggcgatcacc tcagtgagct gcatcgtcgc atcttcggcg gagcggaccc
    16741 cgcaggcgac ctcctggcga aaatggtcca tctcgctcac gccgtccgcg accgatgcct
    16801 gcatttcgct gataatgtcg gaaatatcca gcgttgaaat ggcggtttca ttggccagcc
    16861 gtccgatttc ccgcgcaacc acggaaaagc cccttccgta ttcccccgcc ttttccgctt
    16921 cgatggcggc gttgagcgac aggaggttcg tctggtctga aatcttggta atcgtcgcaa
    16981 taatgctgtc gatgctcgcc gccttctggc gcatcaacgc gagtttttcc gaaatgctgt
    17041 ccgttcccgc ggagaggctt tgcatggcgc cctgcagacc ggaaaggctc ttctgaccgg
    17101 cgttggcctg ccgggaggct tcgccggcgg aatttttgac gttctccatg gtgccggcca
    17161 gctcctggga cgtcgccgag atctgtctgg aagaaacccc cacctgcgcc acgtttcctt
    17221 ccagactctt catcgcctct tcctggcggc ccgaggccga aacgatggag gccgtggtcg
    17281 cgttgagggt atcgaccgcc tcccggattc ggatcgtctg ctcccgaatg cttcgggtca
    17341 tgttgttgat ggagcggctg agctggccca cctcgttgcg gctgctgccg ggatcgatct
    17401 ggacgcgcag atcgcctttg gcgacgctgt ccgccgcgcc ggtcagcttt tccatcggtt
    17461 tgccgaccag gcttcgggtc aggaaataga cgagcagcgc gaccgcgacg gccaccagga
    17521 gcatgctgat cagcagcttc agcagaaagg cgaaaatgtc ggagagcacc tcatcgtagt
    17581 tccggcagaa aatgatgtac cagctgtcct ccatgtgaaa ctgcgccgtg gtcagctttt
    17641 ccacgtagta ggccttggtg gtgcccgcaa agtcggacag aaaataggat tcgccggcgg
    17701 caatcttgtc catcacgggt ttcaccagcg ccgggccttt cttgttcagg atcagagatt
    17761 tttgcggatg ggaaatgatc atgccgtcct gatcgatcat cgaaaggtag ccggtcgcgc
    17821 cgatgacgga tttctcaacg aaaaactccg tgaaataatc cattctcggg gcgacaatgg
    17881 tcgcgcccac aatctcgccg ttgtgttcga tcggtgcggc gacgacaaaa atggggtttc
    17941 ctctgaggat gctgggatat acctcgctga tgaagtaagg cttccccgca aaaacattct
    18001 tgatgtaact gaattcgggt ccgcatttcc cgatggtttt gccgcccacc gtgtcgatga
    18061 agaaattccc attcccgatg attttctttt cgccctgaac gtccacggca accgtccggt
    18121 ccggggcaag ctttgcggca aggggaatat tttcataata gggatggcgc gcgtgcatct
    18181 cggacaggta tttctgcgcg tccgctctct tctgcgggtc cgccggggaa agagcgacgg
    18241 caataattct cgggtcgcgg gcaatggtct gcgccgtgcg gatctgctcc tccagccagc
    18301 gcatgagcgc ctgattggtc ttcttcgcaa cgacttcggc tagctcgtaa ttggttttca
    18361 gggcttccgg tttgcccagg gtgtacgcga caaatccgaa ggcaagaaaa agcagcagga
    18421 tggccggaat cagcagcaaa agatactgcg tggaaatttt ccgcgaatta tatttcctga
    18481 ttaatgagtc cagcatcggc gcccttagtc atccgtgatt cgttctttga tcctttacgg
    18541 atgatttgga taccctattg gcgataattt atcaacaact tccgcgcgcc ggacggccgt
    18601 cttgccctgt gtttttttca cgccccggcc gctgatttca cttgaaagga cggggcgggc
    18661 catgtaaaca tacggaccgg cggacggatt ttggggtatg gttcgacggg ctcaccgctc
    18721 gacggactca ccgctcgacg ggacctgccc tgcgtttatc gaagggctcg ccgccgcttg
    18781 ttccttgtca tggttcgaca ggctcaccat gacacccaag attgtcatcc cgagcacgtc
    18841 gagggatggc gggaatatta aaatggccgg ttttacgcat cagtaccgtg aaaaattgac
    18901 gacgttcgag gaagcggcga aagccgtcaa ggacggggac acgctgatcc acggcgtcac
    18961 cattgccgag ccgccgggcc tgctggcggc catcgcccat cgcgcccgcc tgggctcgct
    19021 gaaccatctc aaagtttaca cgttcaacgc ccagaagcac tttgccgaaa cactgggaag
    19081 tccggatatc gccgacgtca ttgactccta cagctggttc gtaagcgaaa actcccgcaa
    19141 catggtccag gtcgggttga cgcaattcgt ccccgcctat ctccatcagg ttccgaaaat
    19201 cgtcatggag acgatggacg tcgatgttgc cgtggccacg gttgccccga tgaacgaaga
    19261 cggctgcttc agcttcggcc cctgcaacgg ctacatgtcc gtcatggcaa cgctgtgcaa
    19321 aaccctgatc gtcgaggtaa acgaaaacat gcccttcgtt tacggcgacg ccctgataca
    19381 catctccgac atcgaaatga ttgttgaaaa taccgttccc ctgctggaat tgccgcccct
    19441 gcagccgaat ccgctggacc cggtcatcgg caagattgtc gccgaataca ttccggaccg
    19501 ggcggtaatt caactgggca tcggcaccct tcccaatgcc atcgcgccct gcctggaagg
    19561 acaccgggac ctgggcatcc acaccgagct tctgtgcccc ggcatggtgg atctgattca
    19621 aaaaggcgta attaccggag cgcacaagaa cgtgcatgcg ggcaaacata tcttttcgct
    19681 ggcctacggc ggaaaagaga cgtttgaata tatgaacggg aacaaaagct ttgaaaatta
    19741 tcccagctcc tacgtcatga atcccggcgt catcgcccaa aaccaccgca tgacggccgt
    19801 gaacgccatc cttcagattg atctgctggg gcagtgcaac gcggaattcc tggaagggcg
    19861 gcagtacagc ggcacggggg gacagcttga ttttgtccgg ggagcctacg attcgcccga
    19921 aggcaaagcc atactggttt tgtattccac ggcaaaaaat gaaaaagtgt cgcgcatcgt
    19981 cagccgtttc ccggcgggaa ccatggtcac cacgccccga aacgacgcgc attacgtcgc
    20041 gacggagtac ggcatcgtca acctgaaagg caaatccacg agggcaaggg cgctggcgct
    20101 gatcagcatc gctcatccca agttccggga agggctcatg agggaagccg aaaatatgtc
    20161 gctcatgtga aaatcccgtc ccgccggcta aaagaagaat cccggcggac gtcaatccaa
    20221 cgagcgttga ggaccggatc atgacttcag aaaaactgca aaaagaaatg gaccaaatgc
    20281 gcaaaaccct gaaccgcctt caggaatccg acgcgaagca cagagaagcg gcggaggctt
    20341 tccggaaatg cgagcaggaa taccgcgaaa tggtcgagct ggccaacagc atcattctgc
    20401 gcatggacac ttccggcaaa attctcttcg tcaacgacta cgcccttcaa tttttcggct
    20461 tttcaaaaga ggagctcatc ggaaaaaatt tgatcggaac catcgttccg cagatggagt
    20521 cctccggacg cgatcttgcg gccttcatgg catcggtctg ccgaaaaccc ggcgaacaca
    20581 ccaccaacga aaacgaaaac atgaaaaaag acggcagccg ggtctggatc ctgtggacca
    20641 accggcccgt cctcgatgaa aaaggacaaa tcgccgaaat catttccgtc ggaaacgaca
    20701 tcacggaaaa aaaggaggcg gaaaaaattc tgcgaaacgc ccgcgacgaa ctggaacggg
    20761 aggtgcggga aaggacaaag gaactcaaga agctcaatga ggatctgctg ttcgaggtgg
    20821 ccgagcgcaa aatttccgaa gaggccttaa aggaaagcga agtccggtac cgcagcatcg
    20881 tcgagaacgc ctttgaagga atttttcaaa ccaccctgga aggcaaatgc acgatggcga
    20941 atgccgccct ggtgtccctg ctgggctacg cctccgccaa agaatacatg gccgcaacgt
    21001 cccgtccaga aaatctttat gtggaggcgt ccgaccggca ggaactgatc gggcttctga
    21061 aaacggacgg gtacgtgaag ggttttgaaa cgcaattcta ccgcagggac ggcggcaaaa
    21121 tatccgtttc cctgaatgtc cgcgccatgt tcgacgagcg gggcaatttc atctattacc
    21181 agggaaccgt catcgacatc agcgcggaaa tcaccctgcg gcgcattctg gacgaaacca
    21241 ccggcgcgct ctccacggcc gttgaagtcc gcgaccccta taccgcgggc caccaggaaa
    21301 gggtgacgcg cctggccgag gccatcgccg ccgaaatgaa tttcagcgac gaacacatca
    21361 gcgccgttca cacggcgggc atgctccatg acatcgggaa aatttacgtg cccgcggagt
    21421 ttctcagccg gccgggcaag atcacggccg aggagtacag catcctgaag cggcacgcgc
    21481 aagaaggctt cgaaatcctg aaaaacatcg agtacaagta tccgattgcc gaaatcgtct
    21541 atcagcacca cgaaaggctg gacggctcgg gatatccccg cggccttcgc gcggatcaga
    21601 tcctgatcga ggccagaatc atctcggtgg ccgatgtcgt cgaggccatg gcctctcccc
    21661 ggccctaccg gccggcgctc gggatcgaaa aagcgctgaa tgaaatccgg caaaaccggg
    21721 gcgtcctcta cgatgaaaac gtcgtcgaca cgtgcctggc gctgtttctg cacaaagaat
    21781 tttatttttg aacaggaagc cggtggaaaa aggcctttca aggggaagga cccggcaatg
    21841 ctttccgcct acagagaatg cattttgaga gaccggccat agatggtctg gccaagcttg
    21901 accgtcatct ctttaatcat ggcaacttcc tttgatgaaa atttcagctt tgcctcggtt
    21961 ttttcggccg tcacggaaaa gccgccggct gcgttgcagc cgtgcatccg gcaggaggcg
    22021 ggaaaacggc gctcaggttt gtcggcgtaa tccggcgagg tggtgtccac caacccgtcg
    22081 atcgatgata ctttaatcgt gtatcccttc ttcttcaggc ccgcggacag ttgatccagc
    22141 aggcgggaaa ccttgtcgta gggccggatt ttttcaaaca tgaaccagag agtaaccctt
    22201 gggtgcttcg tcatggcggg ggcatccgga tcggaaagga tttcgctgaa ggttccgccg
    22261 tgccccgtcc tgctgtcgaa atccccgaaa aatcgcatcc cgaaagcgtc cacccgctcg
    22321 ccgctcattc cgaacttgaa ttttagtacc tgcatatcct ctcttttccc gtctgaatct
    22381 ctcggcgtcc ttcgtttttc atccaccgcc gcacgcacat atcatatgtc gaagccaatc
    22441 gcagtattta ttttgttttt ttgtcccggt cacggttttt tttctcccgt ccaagttcgg
    22501 ccaactgtcc ggccagcaca tgaaggaaat aaatcaggcg cgcaaaaaga ttggaaatat
    22561 cgaaacaata atcctttacc tggcgcgagg ccgcttcgtc gcgttcaaaa tgacgcgccc
    22621 gcagggattc catcatcggg ccgcgatcta aggagttgac cagatcaacc gaagcggcgg
    22681 caggcctcgc gcaaaaaagg tccccgaact ggcgtacgcc ttcctccagc aggcttgcgg
    22741 catcggacag ctccgccagc attttcgcct cgaacaccag gttgacgcag agtcggcggg
    22801 ccaccagaac gtgaatgtcc gcgcgcgcac gggacagcat ctgttgggtc tcgtccgcaa
    22861 aagccttgtc gtctgaagac ggcgtccgcc ccggcccgcc ggcaaaaaaa gccggatagc
    22921 gcgcaatgcg gttgatgccc tccttcaaca aaccggcaac cctttccagc ccgcggtgat
    22981 tttcttcgac gttgccgtct tccagggtca aaatccggtc cgccagatcg agaatccggt
    23041 tgtcgtgcgt caccatgagg acggcgctgc gcatttcccg ggccagacgc tgcaggagtt
    23101 ccacgatttc acggccggat gttttgtcca gagcggccgt cggttcatcg gccatgataa
    23161 tggacggttc ccgaaccagg gcccgggcga cggccacgcg ctgccgctga ccgccggaaa
    23221 gctgcggcgg atgtttatgc tcatgacccg ccagtcctac ccgttcgagc atggccatcg
    23281 accgcgaacg gatttcagca atcgtcaggt cggtgcggtg aaccagcccc atttgaacat
    23341 tttcccgggc attcaaggat tcaagcagat tgtgggcctg aaagatgaag ccaatctgat
    23401 gacgaacggc cagcagcctg tcgggcgtcg cgccccgcaa ttcaatcccg ccgatgacaa
    23461 tgctgccctc ctgaatggag cgcagagcgc cggccagggt cagcagcgtc gtttttccgg
    23521 agccggaggg ccccatgatg atggcgattt caccggggta gaaatccacg ttgacattgc
    23581 gcagaacctg aagacgcacc tcgccgctgc caaacgtgtg gctcaggtcg ttcgcggaaa
    23641 tcaacgcttt ttcgtggagc gcggcagtcg ccataggcgc atattccctt taaaaaatat
    23701 cggccgggtc aacggaacgc agccttcttg tcgctaaaaa acccgcgatc aggcacatga
    23761 tcgcggccat gcagaaaacc agcgccgtgt cgaacagggt aatccgggtc ggcagttccg
    23821 ccgacgactc ggccagtaaa aacaaaagag cggtgatcag caatccggga ataaacccga
    23881 tcaccagcag aatcagggac tccgtaagaa tgaatttaac gaaataaccc ggtccgagac
    23941 caatggccgt cagggtggcg tattccttca aatggtcgtt gatgtccgtg taaagaatct
    24001 gatagacgat cacggcgccg acaaacatgg ccaggatcat gccggcgatg gtaatgaaac
    24061 ccaccggtgt gttgttctgc cagtaaacct gctcccgctt gatgagctgt ccgcgcgtga
    24121 gcaccagcac atcttccgga acaattttct gaagacgctg agccaccgct tccggatcag
    24181 ccccgggctt gaggcggatc atcccgaggt tgatggttcc ggacggcaag ccgctcaaac
    24241 gctgataggc ttccattccc acaatgacgt gtccgtagga cgcgagcgtc tgtcccatgc
    24301 tgaagatgcc gctgacccga atgcgctggt cgttgatttc cgtctcgact tccccctgct
    24361 ttgcaaacag gccggccacg tcgccgaaat ccggcgaaga tccgtcatcg aacaaaatgt
    24421 tttccggact ggtcaggaca tcttcgcggg aagccacttc cggaatgata aaaggattcc
    24481 tgccggcata gacgccgatc atggccgcgt cgatcttgcc gccggtctcc gggttgcgcc
    24541 acttttgata ggaaagggac accggataaa ccgtttcgac atcgccgtcg gaaatcgtct
    24601 ggtaaaggcg ccgctcagga aagtgccccg gcgtcattaa atactggtaa tcaggactga
    24661 tcagcgccag ctctccctgc atcgccacaa acggccgaat gaccatgatc tggaaggcct
    24721 gatagatgcc ctgctgaaag agcatgatga ttacgccgaa agaaacaccg gcaatcgccg
    24781 tggcaaagcg ttttttgtcg tagctcaatt gacgccaggc cagagaaatg ccgcccggcg
    24841 ccagccaggc ctgaatgcgg gaaaaaacct tcatcgggta tgcaggatga tagtgacctg
    24901 ggcgttgctg aacggggcca gacgctcagg cttgtccggt ttgatgcgga ccatgacaat
    24961 gcgccggtcg gcatggcggc gcggatccag ggcataaatt cgccccgctt caactttccg
    25021 ttcaatttcc accacctgtc cgcctatctc cccttcgaag accgtggaac gaatctgcgc
    25081 ccggcttcca atcctcacac gggaaatgtc ggtttcatag atttccgcca gaatcatcat
    25141 ctttcccgta tcggcaagat ccagaattcc cgactcgcca atcgcctcgc cggggtaagc
    25201 tcgaatggac aaaattctgc cggcgatcgg agcgcggatg cgctgcgcct ccagcagggc
    25261 catctgacgc cggtagttcg cttcgaccac ggcgactttc gcgcttgcct gtcggatttc
    25321 ttccttgtgt ccggactgca tcccttccag aacatttttc tcccgcagca aatccgcctg
    25381 ggcggcctca aagtcataca gaacagcctc ataggcatca tccggaacaa tcgatttcgc
    25441 gtggagctgc tgataacgct ctttcttgga cttgtgcatg cgcagcgccg cttcacgggc
    25501 ggccacaacg gcctgctgcg cctcgacctg ttcggggcgt tctccttttt gcacaagggt
    25561 cagaccggcc ctggcaagac caatgtcccg tctggcgcaa tccaccgccg ccgcctgtgc
    25621 atcatagtcc cgcaatcggg ccagttcggc cccccgggcc acctggtccc cgcgcttcac
    25681 cagcagccgg tccatgatcg ccccctccgg cgccgacaga acaattgtcc gctctcccgc
    25741 aataatctgc cccagacagg atatgttcgt tttttgttca gccgcccgga cgggaaatac
    25801 cattccggcc agaatcagaa ccacgacaaa caaccgagac gcttgaatcg tatattgatt
    25861 atgcatgcgc gaaccataag aaactcggtt tattctgtca agaaaatcct gaacgcaaaa
    25921 cctattcctt gcaggatttt atggatgagg gaagatgatt cctcagaaga tcggcaaatt
    25981 gttcggggga gacgggcgcg ctgaagtgaa acccctgcat ttcgtcgcag gcatgcttct
    26041 gaaggaaggc catctgttct tccgtttcca cgccttcggc aacgaccgtc aggctgaggg
    26101 tcttgcccat ggaaaggatg gccctggcca cggcgatgtc ttcgggattc tccatgatgt
    26161 tccgcatgaa ggaccggtct attttcaatg catcgacggg aaaatgccga atctgcgcca
    26221 gagaagaata acccgttccg aaatcgtcta tcgcgatccg cacgcccagt tttctgatgt
    26281 tgttcagaac ggcgatgatg cgcgcgggat tgtgcatgat catgtgttcc gtaatttcca
    26341 gctccagcag atggggcgcc agaccggact ccctgagcac cctctcaaca tccacgatca
    26401 gatggacatc gttgagctga cgcatggaca ggttgacgga catgcacacg gggggaagcc
    26461 ccattttttg ccaggccgca ttctggaaac aggcggttct caaaacccag cggccgatgg
    26521 gcacgatcat tcccgtatct tcggccaccg gaatgaatcg cgccggcatc aggaggccga
    26581 gatcaggatt atcccaccgc agcagggcct ccactccggt aattttgccg gtgcggatat
    26641 ccagccgggc ctggtattgc agaaaaaatt catttttttc cagggcaaag cgcaagcggg
    26701 tctcgatcga caatcgtctg gcggattgcg actggatgtc atgggagtaa aacttgaagt
    26761 tgtttttgcc gtcctccttg gcgaaataca tggcgacgtc ggcgtttttc atcagcgttt
    26821 gttcgtcttc gccgtctttg gggaacaggc tgacgccgat gctggccgta acgcggcatt
    26881 cctcgcccat aatggtcatc ggctgaaggg cggcggaaag aatccttcgg gcgacggtgg
    26941 tgacatagct caaatcttcg agctcttcga tcaaaatgac gaactcatcc ccgcccaggc
    27001 gggcgacggt gtccaccgcg cgcagcttct gtttgaagcg aacggcgatt tcccggagca
    27061 gttggtcgcc tgcgtcgtgg cccagcgtgt cattgatcat cttgaagcga tccagatcga
    27121 tgaagaacat ggcgaacatt ctctgatagc gcttcgccga ctggagggcg cgctgaagaa
    27181 gctggttgaa aaggatgcgg ttgggcagcc ccgtcaaccc gtcgtgcgtc gccagatact
    27241 ggatgcgctc ctcggatttc tttcgttcgg tgatgtctcg cccaatgccg cggaaaccga
    27301 cgatctcccc tttcgagttg tgcaggggaa acccggaaat ttccgcatag cgcgccgatc
    27361 cgtcctttcg cgcatacttg taaaaaatgc ctctctgcgg cttgccggtc gtgtaaattt
    27421 tgccgaaggc attatacaga attttggtgt ctttcttatc cacctgatca cggaaagttc
    27481 tgccgagcaa tttttccttc gggtagccca gaagacggct gatcgcatcg ttcacaaagg
    27541 tgtaatttcc ggccagatcc acttcaaaat aggcgtcatc catttcttcc agaatggtcc
    27601 ggtatctctc ttccgactgt cgcagcgctt cctccagaag cctctgcaac gtgatgtccc
    27661 ttccaattcc gcgaaaaccg acgatctccc ccttcgcgtt gtgcagggga aacccggaaa
    27721 cttcagaata gcgcgtctcg ccgcctttgc gtgtggactt atagaccatc ccccgctccg
    27781 gctccccggt cgtgtaaatt ttgccgaagg cgcgatacaa gagatgcgta tcttccttat
    27841 taacctggtc gcggaaggtt cggccgatca gttcttcctt tgagtatccc agcaggcggc
    27901 tcaaggcgtc gttcacaaag gtgtagttcc cggccagatc gacctcaaaa taggcgttgt
    27961 ccgactcttc caggatcgtc cggtaccgtt cctccgaccg gcgcagggct tcttcggcct
    28021 gtttgagccc ggtccggtcg cgcaagatgc cccgaaaagc gacaatccgg ccggcatcgt
    28081 ttttgacggg ggtgattgaa ctttctatgg atttgcgggc gccgtctttc gcgatgactt
    28141 catactggac atctttttcc gattgccccg tcgtgaacac ccgatgataa gaggcaaaca
    28201 tcttcgcggc gttttcttca tccatcagcc ggcggtaatt ctcccttgcc agttcttccc
    28261 gggggtaccc ctgaatgcgg cacagcgcct cgttgaaaaa gaccaggttg cctttcagat
    28321 ccagctccgc gtaaccgtct tcgatgcctt ccaggatgga ccggtatttc tcttcgctct
    28381 gacgaatgat catctccatc cgcctgcgtt ctgtaatatc catgaagctg cccagcgcgg
    28441 cgcgcgttcc ctcgtaggga atggaagcga ccctctcgag aatccacatg acatcgccgt
    28501 ttttcttgat gaaccggtac tcataaggat cggttcgctc ccgcttcaac gccgcaattg
    28561 cgttttccct cgtcttttct ctgtcttcag gatgaatgaa ttcaaggcaa tggtggtgaa
    28621 gcagctcttt ctccgaatag cccgtcatcg tctggtacag cgagctgaca tacacgaacc
    28681 agcctctctg aacaatgtag atcgctacgc cgacgttgga aagaatgtct ttggaaagat
    28741 ggtagatgtc gttggaagga gaaacccgtg cgccgccggg agaaaggacc ggtttggagc
    28801 gggcttttga cgttttgctg tttgccgtct tcttttcggg tacggttttg gatgaacggt
    28861 tccgattgga gctatccgcc ataaacaccc gacctccgct tcccacgcat gaacaaaatc
    28921 ctgaattccg agcgtcaccg tgagttgtcg catcaacaca gaaacgaaga acaccaagag
    28981 catgcatccg atgcaggttt tccagttcgc cgatgggcgg actttccggc gcggatgaaa
    29041 aacgccgcct ggatggaaaa ggacaaaaag cgcggaactg cagacacctc ttctccgacg
    29101 cggtgtccgg atggcggcgt aaaaaaaggt ttaacgaaga atcgccaaac ccttaattta
    29161 gttttggtag cgggggtagg atttgaacct acgacctttg ggttatgagc ccaacgagct
    29221 accaagctgc tccaccccgc gacaggaact tataaatttt gtgaccttaa aacatcatct
    29281 ccctggtttg tcaaggagtt atttggccgc aggcgcgcgg aatggagccc gcggcggcta
    29341 agtcgtcaga taccccatga aacgatagat tagtttggcc gccatgaaat ccggcgcgac
    29401 gacaccgggc agaggggcca gttcaaccac gtcaaacccg cgaacggcgc atttgcgcga
    29461 caccagccga agcaaaccca gaacatcgcg ccagaaaaga ccgcccggct caggcgttcc
    29521 ggtcgcgggc atgaccgaag gatcaaaaac atccaggtcg atcgtcacat aaacatcgcc
    29581 ctttatggat ttggcaaccg tttcggccca gtttggatgt ttgaaaacat catcggcgct
    29641 gtaggatttg acgctgcttt cgggaaggaa ttctccttct tccctgctca tgctgcgaat
    29701 gcccacttga accagatcgc agaactccgc cacccgtctg gccacgcagg cgtgattata
    29761 gggcgtcttc tgatagcggt cgcgcaaatc cgcatgcgcg tcaaattgca gaactgtcag
    29821 attcgggtat ttctccgcga ccgcccgcac cgcgcccagc gtgatgctgt gttctccgcc
    29881 cagcatgacc gggatctttt cctgcgcgac gatccggcgg attcgtttgc gcacggcgtc
    29941 gatcatggtt ttcggtccgc gggcgtccac ctcgaccggc aaggcggtat ggattccggc
    30001 caaaaaggtt tctgtcttca gctcctcatc gtagagctcc atattggccg acgcttcgag
    30061 aatggccgcc gggccgcggc ggctgccggg ctggtaggtg gatgtcgaat cgtagggaac
    30121 gggaacgacg acaaacttcg cgttgcgaag agaacattcg gggtatattc cgccgtaatt
    30181 catggttttt ccctcttttc ctcaaaaaaa gtgtggtttc ctagcatgaa gaaacggaga
    30241 tgtccagttt ctcaacaaag ccttcacgcg cgctttcctg accggccgga taacgcatca
    30301 taaagaattt cccttcctca tagaataaat acgagcggtg gatgatccag tcgccgaggg
    30361 ccgcgaatat tttttttccg ccttccccgt ttgagcagcg cagcagcggc ctgtggcaat
    30421 gccctagaat caccgcatca tgattcgtaa agagcctttc gacggcgcag gcatacattt
    30481 tttccgccaa aaccgctccg tcttcgatcg tgaccgtttt gctggtcgcg gaactgaggc
    30541 cggccgccgc ccacagaatc ggcgaaggaa tcaggcgctg cagatgataa aaagtccggc
    30601 tgcgcaaaac tttccgcagc gtgagataaa aaacattggt ccggtccagc gtgtctccat
    30661 gggcggcaaa gacacgtctg ccgcccagat caatcgatgc cgattcctca acgacgtcca
    30721 ttcccagcac atcgtaaaaa tattcccgca tgaaaaagtc atggtttccc tcaaacaaat
    30781 ggatgcgcac accgctttgc cgcaattgaa ccagttggcg gatgaccggc tgaaattccg
    30841 ggtaaatccg gtcgggccgg caaaaccaga aatcaaaaaa atcgcccaga atatacaaat
    30901 catccaccgt cgttcccgcg ccggccgctt ctttgccggc ggcggccggc cctaccctgc
    30961 cttccccaag atccgtcagg aaacgaacca gcaggccgta gggctcgtcg gttgagcgct
    31021 tcaaatgagc atcggacagg aaaacggctt tcatcattcg gaccttcaaa aaacagggac
    31081 agcgcctctt tattacaagt tttaaaattt aagttttaag tgttacgtta ttgacgtctt
    31141 tctgcaggga acgctcgcga ccacccatgc gggtggcaag accgtaccct gcccaaaggg
    31201 agcagcgtcc tgctgtttta ccatttgaat gcagggcgta ccttcaggtg cgccatatga
    31261 tcgtttcaag taagttcggc aggcctgata accccgccac ctaaaggagg cgcaaggtca
    31321 caagcgaccc gccctgcatt atttctccaa tcggcattct tgtgcgcaac catgatggat
    31381 tttccggcgc ctgtcaaata aaatccgcgg ccaattgatt cttgttttgt ccggtgtttt
    31441 ttgctaacta tccatcagcc cggatgaacg ggcaagaaag aaatcaaaag caggaaaaaa
    31501 acatgagcag cagccgaacc gccatatcac gcgccagaga agttctgaaa atagaagcgc
    31561 agagcctttt gagcctgatt tccaaggtca acgggaactt ttcccgcgcg gtcgatctga
    31621 tctatcagtg caaggggcgc gtcatcatca cgggcatcgg caaatccggc ctgatcgg
//
LOCUS       DAOSIM010000002        27160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_1969_length_27160_cov_6.754178, whole genome shotgun sequence.
ACCESSION   DAOSIM010000002 DAOSIM010000000
VERSION     DAOSIM010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 27160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27160
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1969_length_27160_cov_6.754178"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1714
                     /gene="yidC"
                     /locus_tag="PLR47_00145"
     CDS             <1..1714
                     /gene="yidC"
                     /locus_tag="PLR47_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOG11369.1"
                     /translation="KKLGGMFMDKRTILAIVLSLAVLLVYQIFFVKPPVPQKGASSQT
                     PAGQIQQDKAAAPSPAAALTKAPAAPAPKAAAGPLTPPRDITVETDHYTAVFSTRGAA
                     LKSLKLKGYYRDCHECTEDIWPKIKGLVTGQKASPKPKTPEFVELVDVREGMPYPLAV
                     TFPESGVDVAPDEVFDAAGNRMDLTGTQQKQQLVFSRSQGGVKIEKIYSFDPSGYTLG
                     LDVKVHNLTGAPITQIPQLNWYQYVDPQKIDDSYGHEGPIVSVGGSIELEEVKKLQSQ
                     SVQGPNVLWGAFESKYFIASFIPENPSLTNAVLTRDAANMVGVGMKGRKEIIPGGQNS
                     VFSYSLYLGPKQYDLLKNLGVGLENAIDFGWFKWLAIPALYILNFLYGFVLNYGIAII
                     ILTTIIKIVFWPLGNMSYKSMNEMKKLQPKIEALKQKYKNDQTKIGQETMALYREHKV
                     NPFSGCLPILIQIPVFFGLYKALMYSIELRHSPFFFWIQDLSAKDPYYITPIIMGATQ
                     FITQKMTPAMGDPTQQKIMLFMPVIFTFFFLNFPSGLVIYWLWNNILQIGQQYYINKK
                     LATG"
     gene            1732..2724
                     /gene="jag"
                     /locus_tag="PLR47_00150"
     CDS             1732..2724
                     /gene="jag"
                     /locus_tag="PLR47_00150"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOG11370.1"
                     /translation="MSAKILDIEEKNIDAAIERACREFGVGREKLNIEIISEGSNGFL
                     GIGAKKAKIRASIFSFDMDFSTEDSARQKERPQAKSDGKPEEKTDRPAERKPESRQEH
                     RQEKRHEAQPERRAARSAPAESRASAPRPARVKPAQPPAAPEQSPPPAVPRVEAVPAT
                     TPAALKARDLLAGILGRMTFECQVTAAETDETIILSIEGDDGGLLIGRRGQNLDALQY
                     LLNKSVHKSEAQRKMIVVDSEEYRKRREESLLAMAERIREKVKKTQKPLSLSHMNAHD
                     RRIIHLALQEDEELITKSRGEGEYRKVIVLPAKKGNSLRPGKNRSRKEPAGDSF"
     gene            2756..4138
                     /gene="mnmE"
                     /locus_tag="PLR47_00155"
     CDS             2756..4138
                     /gene="mnmE"
                     /locus_tag="PLR47_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOG11371.1"
                     /translation="MVTKTETIAAIATPSGTAGVGLIRVSGPLAPELAQRLFRPAHSN
                     CTWQSHHCYHGDIIAADGKTVLDEVLATLMRKPRSFTGEDVLEISCHGNPLILQNIME
                     QFLALGCRPARPGEFSERAYLNGRMDLSQAEALAAMICAQSAKALQLELSQLKGSLGN
                     SIDELRSLLIDGLANLEAAIDFTEDVSDKEIPAVPGQILQAAAGVELLLSTYRQARLI
                     TQGIRVVIVGKPNTGKSSLLNRLAGKKKAIVTDIPGTTRDLITETITLDGISIHLTDT
                     AGIRPPRDDIEKEGISLVRQQMEQADILLLLFDGSQPLTDEDYRILEQSKKRDCQILT
                     VVNKRDLAPAWDNRLPGHPLHGRRLLDLSAKFGDGVEDLKKALSDLAGGTDAPAEGGM
                     ISRLRHKLALEKALACLKAAQNCLATGQSSEFAAFELSEALNALDEITGKKISEDVLS
                     RIFSGFCIGK"
     gene            4238..5371
                     /locus_tag="PLR47_00160"
     CDS             4238..5371
                     /locus_tag="PLR47_00160"
                     /inference="COORDINATES: protein motif:HMM:NF027862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein T N-terminal
                     domain-containing protein"
                     /protein_id="HOG11372.1"
                     /translation="MKTSPFLFCVLCLLCFSVPDAPAQSKCVHADGEALILNNDRPSA
                     KMEAVARAKWTAIEDVAGTEVKAGSLVQNFTLVEDVMKTKAGGVVRSYQVIDESVKDD
                     VLNVKINACVEPSQARNAVSQLGLNNSIALFIPARKPGRRGDEFEEKNILSETLIGKL
                     TEQGFNVVDVAPTHAVNARDVESAVRTGRTLSMRSLTYKFLSNLIIIGKVDYTISTKK
                     GEDIGYGLSMPFNHVTVRLNYRILSKNNNTGNMEILSTGVEQARGLANSVEDAAAQAM
                     QELAETLTPSLLDKVGQYIQGNTKKIAVQVAGVEDLDAVLKVKGILQNIVWVAGVEEK
                     EMGSFVVSYPENTLYLANSIQQKGSFKIVNFSPYSLTLEYLKQ"
     gene            5384..6454
                     /locus_tag="PLR47_00165"
     CDS             5384..6454
                     /locus_tag="PLR47_00165"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOG11373.1"
                     /translation="MFNSRGRYGNYPVLVSAILLVTLLMLCGCATKIRVNMLQPAQYH
                     EASLTKAVAVLPFTGPGGPEFAAEIEGVLAGIGIDDKPYFTLVDRASVDKIVSEMKFS
                     HSGLVDPKTAVKLGKMIGAQGIYTGVVTQNNHDDSFYYEKRSTCVHYEKRRDEKGRIY
                     QGACLQWRNYNVRCMKRVANFAVSPKLVDVATGRIVYSRNLSAVTASNGCEDTRPVQS
                     ESVLLEQAKASVKNQFRRDIAPFYVTREIRLIDSTDGIESNEAKDLLKRGLEYADKGR
                     MDSACELWGQARNLTPGSYALLYNLGVCAESRGDLDTALTLYRQSDRALGKPDDDITL
                     ALSRVGEAIKNRGKLQEALGVK"
     gene            complement(6459..6965)
                     /locus_tag="PLR47_00170"
     CDS             complement(6459..6965)
                     /locus_tag="PLR47_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOG11374.1"
                     /translation="MNIFKRILPVVLLLVMTVSGADAMAQETSKNLSVGKKASGKTSF
                     LETQKAVRETLDGMVEAYTGKNARQFMSFVAENYMGDDSLLERRIRRDFSKFLDMDIR
                     YTLNNVTTDSRNENISVAVSFTRSYTDVKTTKRINKNGSAAFIFRMVDGRPRLFNMKR
                     PYMFGVGK"
     gene            complement(6994..10533)
                     /locus_tag="PLR47_00175"
     CDS             complement(6994..10533)
                     /locus_tag="PLR47_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11375.1"
                     /translation="MSVRRDWKLIYKRGRLFLLVILATLMLVNSAGAFSTDFEAGVDF
                     FGNFVEKGTNSGYGTVLKLDQPNLDPDKFKNFDWARLTMARFSTALDVVDKLNRGEYD
                     DAQLALALRAIQEIAGSESGGKLLGALGLSSLTVNVFVVSVKVYVASVRAVRESTRAV
                     QLESLYGHFESIANNPRLRNPGRVLGEGDPMPVTRASIDLLWRDIVDDENVRAQFYVY
                     VTQLLQRDWPEGPYWLGWYAAAHPPQKLPSAPNTVVQTITATPPPPFLQAQPVAGPTP
                     ELLASRRREMEGYIASLLGDLNQKRKVEEQLTQVRKSFIDLKDRIGRSRDLEAIMVKF
                     AQSLERLPAVEEFGKKCPGYIQNAENIKSQRSLEDLMEQSKGFAENVIQFIPAKGFYA
                     KDRARLLGLLKTCYIQAKEKIPVVAQKQKEEMEKPTPRPTDARKDETVQDGYAYYFKA
                     FVVPYAWSEEPDTAKRSVFVSLSKGKWDQADDVYARWTGKTYYGYDRGPGQTYVVHYS
                     DVLTKVTDLYNKPRNPLAKQYQAAYDSCDAAYRASFGPRDACYAAAKTEKDKQPCQDI
                     ESRASNAYSFCQESSGRPLRLQIEVLDAGFSRAKQAVGYLLEGDESLYRETKVAMDEA
                     FRSIVELQQEKKRQLETLRKDLAKFDGRLPLEEKPRVVEGLDRAIADLKKNSWPGVTK
                     EWMGGGGISSSSVVIDERALSLRERARPLPGSVDSWEAKIIEAEEGWNFAVRDWQAFY
                     PTVDLKKQDYDMITMWVDKTFSRDDFDRYDRAANRVPALVYSAKTKLGEYRTLAGTEF
                     HNISTDAAWLSSITAEFSKWVAQKQKEGIPYFSYSPGLHLLSSPYPHYLTKGEAALYA
                     AEAKQLKIYDFVQKYMPSSLAAFDADLLLGKIRLAEEDNFIIGFNTIAGNIAIYKSQI
                     SRANDLLASLKPDMGAVAYGEVLKEVSTVLPVTLERSNEAEDKAREKNAKSMKISYVP
                     LPWEGRLIFVDESKLNFPLGLAYLDLRKQLSVAIRKGQEYTIAERAKAAEKNGQQEQA
                     KKESPKTAEVAPSPAVSAGRDKEEIQAFYNRFKQAYEERNDSQVLSMISDQWQANDGA
                     TLSDLQMNLRRTFKMFDEVKYNIQNLSMILVESGNGRLRYLVSYDVTITSRMYKRNLR
                     HEEKSSINEEVTIEKSGKAKISRTLGGRFWYVQ"
     gene            complement(10585..13860)
                     /locus_tag="PLR47_00180"
     CDS             complement(10585..13860)
                     /locus_tag="PLR47_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11376.1"
                     /translation="MMSRLLISNICRSLLLLFLCCFNPLAADAQEYQQAANALENLAS
                     EIRMNEAAVGQVLMEAMQSKHGDMQKWNDLDAKMAELGRSIDANLPEISRNAAIVLAQ
                     NPDQYVPSISLGRTFEGNLRMATDKRKQLGEQWKAENDKLMSINAKIAKVTELIGNAQ
                     AGSAIDAVMVLNPLPDDFWEFGGEATIAVLALYSGPVGWVALAGTAAAKGISMVTKSY
                     YNAKSLKERLKVYTELRDVLQEKKKIHEDNMKTLRDGEQEMRQIEQTLEKHEKKMTEY
                     KARVNSAMEALNNQYKSAFDARKQKLDEEVRKMADAPKPDLKPNGWVYGMDPIPPISS
                     GEYSGEVNAMISQMRSYAQAVEDGGDPDNFQIMVTDWHNRLHDRHKNIKKELDQKREA
                     HNQATQVFWRSYDAASAEYDRAWRIYVDCRDGSDRCYASLRAAGDRYNAAVRAAYAAL
                     RPFGQAMINPYRELIKIDQIHYRVRDAFYPFRERVDNAAKKNTRDFWNQSRLWETKFD
                     ETNAKVYDAVAPVPYWIDQWKSRAERLDDEIQSALYWGRNIADVRAELLSTAKDLRDL
                     HKTVQEAAKKYESANQERIQTANKAQSELNGILTRYGRLINYYWASNFSIDWLGDPME
                     FTPRAPDQEKNITSLEEYVKKKFTFYEPEHLKKSLKLDIPGIAAQYENKARELTFYTD
                     WVDTYRHRASTAVGALDRISVEKTGQYFYSDRGGTVPEVLSKEFGKPPWSTIAQDAEK
                     YVSKGDFASLPWGKFQSWENLGIWQKLYAGQTLLLNKLNKDAKNYVQARSNGWFMAVP
                     AQTMDPLIENWKKLREVSERFDKLAKPEHDKLAGGQEQAQKEAQPVFETWGKMPKYSR
                     DMVAEEYRRFSNAYNWLQDYIRSKFDATRTTLQPPNNSVAVNLDNLILGYAPAYEKWK
                     KQQEEADRRAEEQRRQWEAAEKARLEKEKKQAEEEAQRAARAIEEEKKRAQENLSLVK
                     DFYVRFKEAYEERNDSRVISMISDQWQAGDGTTLSDLQMTLRRTFKTFDEIRYNVQNL
                     SVTPLQGDRYSVSYDVTITSRIYKRNLRHEEKSSINEEVTIEKSGKAKISRTLGGRFW
                     YVQ"
     gene            complement(13873..15150)
                     /locus_tag="PLR47_00185"
     CDS             complement(13873..15150)
                     /locus_tag="PLR47_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11377.1"
                     /translation="MRRKIQFFGIKPVCLLIVMTIVLCFSGQCLAKGPAPKKAKAAQR
                     QEAPKAAVLPAPAVGKAEPEKKPAPPASFGVAAPRDAAAFFEEARKLAATDPVSAVAL
                     YQRGMMLKPDAWKERKELAALYEKQGQWNMALEEYEAVFRADKSAESYANRLRMLEKA
                     GYLRTAAAEARKAFAQYPDRPQFLLQAGELFHKAGDEAPATAALQEYLKLKPDDGRAL
                     MMLGSVYEKSKKPADALNAYLQAEKKMKGNQEAADAAKRLRAGNAVAGGLTIFLPEGW
                     SADQDGIFNLQSGERVTVSVKSAGSPAALALTSARDSMPKEPFSAENLKQMEKLKKMR
                     QELAKKDPESAKNMPSGMMPIHTQGDFAQLKGAKKAVLSTSETLQPGMESAVSVAVPS
                     GGKIYVFLWRASLPAADGEKTLSLLLGQSVWPL"
     gene            complement(15203..16672)
                     /locus_tag="PLR47_00190"
     CDS             complement(15203..16672)
                     /locus_tag="PLR47_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11378.1"
                     /translation="MKYKSKSLIMVMSFALVLLSFAGAQAKWWIFGESDEEVSISYLF
                     VNKSSFDETGAKITLFKEMLPDGKITISGKAVAGKGKIGSARVSLDGKETWKDANLSE
                     GGAFEYSFSPETGKTYSIFVEVSDTLGKTNDVAATARQLTLSERNIMSFIREALDKLI
                     AAYENHDAKTFMSLVSEDFAGDMTNLDRAIRKDFSSFDDIDLNYTLNNITSDSKGISV
                     SLSYRRLLMSTKTGQSVKDKGVTEFVFKMGDKSARIYSMKNPLIFGITGAPNVATGTV
                     ASTTNEPVIIADGRGNVALVPAQTFNQIVMDDSVRITNNPDGTSTIKTSDKTITVDKN
                     GQQTSGGSNPSETVEKGLRTIISLGHPPMGFSFVNGEVMTAAGDFMITGGGPGFGYGF
                     VEPSVTFVDLGAKSIGSVNEAPATGYANPAGMGVRFEEGHTYAFKLANGKYGLMEVKS
                     VTEEWIPGNPGPPPQPPAMRITMRFEYKYQPSGARQFLP"
     gene            complement(16697..18271)
                     /locus_tag="PLR47_00195"
     CDS             complement(16697..18271)
                     /locus_tag="PLR47_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11379.1"
                     /translation="MCFFFLGNPFLLKAENEFHASNEFSYTYNDISGPGSSQSSLRQG
                     FSYLNLLNINDLGNYKGFDYNFNFGLKLTDDPTNDQQTASITNLQGRITNKVHTLNLG
                     DTFETFSQYALSSAVKGASYRFVKENSYIPEVSLVYGYAYSRWDNAFAIYDGPVKAVA
                     RQVMGARLKENLGQNFWAGLSYVQSQDNDRVNPTDSLYLNRAYTFDMEYKPIPGLTVS
                     GEASLSDQKEWTGNDGSETTRHGAAYRLTAVGDADPSRVTLEYERISPNFTTLVGSAT
                     PDREKAKAKWRYKYTKNISANLGFLWFRDNLDGQKTAGRTDYYKPEIGFTLKRFLGRA
                     YGTADIAYKLDVTSNNNTENYNHIVTLNYQDQFFGFLDSDTNLGYTLYDNKISVEERN
                     NEFTYNTSLSSRHTLGMVVLKPAVYLGGWTQRSELVVSTDQIYEYSAGFGLDVPDWKL
                     TSTIKAGQNRLDKSVGQDTDKTFASFNLNYRPPFLKKLNQGMLFFRAKYNDFSYTNAG
                     NNFRETSLTAGLILEI"
     gene            complement(18478..22191)
                     /locus_tag="PLR47_00200"
     CDS             complement(18478..22191)
                     /locus_tag="PLR47_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11380.1"
                     /translation="MKFCILIFILCLLAMPGLCKAASEEDVYDAMFNADASLFTTGYG
                     VFLDQVKDGQRFGASIDVFNKAAGFAATYSSVTTKAFATGDVSGAGEEAMLAVLSEIA
                     GWPSGQGLLKYVGLSTGVFQVALTAYSITKESFAQVRATKIALNIETLYGWIESDPVL
                     KPKKGRQLGDKSDPIPVNNESVEYMFRKVAQDERWRNLFKSYVSEELGETFPEPSTWD
                     WLSIAAVDGWKSAHTAEQAEIFKDKRQVETWIAGLLSQLNKYARAVESEVILRQTLAK
                     IEKASKKITPEFQKFVVGAEEAEKKLPEIDAYAKQVPTIIAAAKKNQNWDSLYDVRDK
                     IGYYARVYVRVLPDTGNIGKKKQATKAALKNSWVDVSKTLKDIPKTIKEDFTAQTVTP
                     QYKTSISLAEVSPDFIDIDEVAARLHKAKSLQDMEAEVILVKEDTEKKYRTFQENHNK
                     ENPAGDQHPAYNSRRDAMLQTLENQRKALEAQIASCDQYCRWLSGANPCGADGGKTWE
                     RCFNSASDAKRAWEKTAWKPLEDLETDKKNYNAKRELYAGNIFNQHEALLKAIGRLTN
                     EERARFADMEKLKKDALDTLGAVDQSVPIFYPETETKHSQVTAADTKQYLNSFRGQKT
                     DGYLGLPKMKFPKWGEDGMAVYEYVRRIERNVSNERQNFDRLSEKHSQAIQYIGKLKT
                     YLKEKDRYEQEIKEAKERIYALQLQSDPKAQVIPDIEKRIAEMKNVEKLALEIEAELN
                     NNLIAENQKQLAGIEDDLQYLDYLFKRIDYWRDAVRGNTLAGSVNATVVEVAVATENS
                     AGSNHHKYLAPSWQDLSLFDKAKAAAAANALLKDVERTGIAKWDPNGDTGLKVMLEKK
                     AAEIRNYCIDTPDYAIVNANVVYAARIARMAADVKAIPASTYGTNTYKEGLKNRTFDE
                     KLGAALGNGTAFAMNIEMKMNSNEQTVSVTLDKAKELAASHGNATIKTASSDMVKAIE
                     EHLSRYNAQVAQEAKQWEIDKAKAEAEQKLQNECLAKGGNYSNGECKTIPGGTGLGTG
                     GFPGQSGQAAGPGAIAAGGPAGINTGAPPAGRGAVMPQPPGSGAKAPAFRPDQTGPAK
                     GEPVGFSASAASRNMGGRSGMDDNLKDKVKDLYGRFKEAYEAKNASGVIYCLSDQWES
                     SDGGTLSDLQQNLRRTFRTFDQIQFRIENMQISNIAEDRFRVSYDAVITSRIYKRNLK
                     HEEKSSVQEEVTIGSSGKPKISKTLGGRFWYVK"
     gene            complement(22370..25465)
                     /locus_tag="PLR47_00205"
     CDS             complement(22370..25465)
                     /locus_tag="PLR47_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11381.1"
                     /translation="MKKMTCLLLICAFLACSQCVFAQDSAEKSSPKTTGEYLKKTAKD
                     QAASTAEDMAKKKVQEKVDQKAPQLINLAYEKYPVIAKFSGRKDFSELMKDYNKYSDA
                     YEKLTTVNSLLVKVAEGNVAGATYDATKEIFTKVFPYSKYIFSYLEAWDKILTSVSDA
                     VMENNLDYIRKEFMRDVLPKCKESCPLSEEQSFAGRIGRRMAEDDQGAYGLVAWYCKE
                     GGAAGSSKCGSICVSGPFDWSKRRSFENSGCEDYAVMSFVRSDQKIRQAVSAHYSILR
                     AIEDVGDMQKAMKNASDYVKKNLLVFEQEQGKIFAAQEALKEQQKKAAQEKIAMAEPP
                     TQCDEKCAESYRKAWYKTREISQEIQDKRSAINARVQEIQKENAARRNELKINVSSGN
                     LEELKTDPGNVSIDEDNVDYNGRQAQRLEKLVANAEKWLQSQQLEIRVYDGRISSLQG
                     LSGLYAQKQNLETQYGTGGTYRSSEKFTANYSLILENEIKGVELKRQELVDEITVVQN
                     NLSGYRSLQSKYKSAFEQGSKKARELLEKEVEPAYQEYAQADQELENIENERREYEQE
                     KLIQINVVMSKITAAKNQAELETLLPQVNKDMAEYNQLLQKQAQLMQKAADKSQKVEA
                     LSRSSRIQRAKGTLGYQSSSLSSRMVRPDLSGSKFSLAAYLPYIAEANRYVVTESARR
                     YGETLKKIPDEAAFIIMPDGDMKKLSMEVSSATGENAASSIKAQASTIARIKDFSGAY
                     SAYQSDLGSSLNSGNWKIDAVLSEEARAALNRLAAFWSEHFNPLMQKRSQKINEKITY
                     SQPKIGSGAQTLHGAVRLTAGDITDGKVPFQITASGWGISRALVRVAAAGKNLPLTIK
                     TSTAAEIPEAVYTAMVPVAAGKATHVSYTVGDWTHSVDLRAPAGHAVSPEDVGKIRAF
                     YDKFKQEYESRNDSRIVAMISDDWQAGDGSTLSDLQMNLRRTFRVFDEIRYNIQNLSV
                     TPGQDGRYNVSYDVTITSRIYKRNLKHEEKSSVQEEVTFGSSGKPKISKTLGGRFWTV
                     Q"
     gene            complement(25628..25900)
                     /locus_tag="PLR47_00210"
     CDS             complement(25628..25900)
                     /locus_tag="PLR47_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11382.1"
                     /translation="MILLFLPDRLVGFPAVNAFMGFAEGAKPAFNLFDKPERGALLFF
                     AGMSAHGAFHADQRHFAIPVRDFIFRGFYGRLSSGPFIFLCGRFSF"
     gene            25931..>27160
                     /locus_tag="PLR47_00215"
     CDS             25931..>27160
                     /locus_tag="PLR47_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOG11383.1"
                     /translation="MVMRHDCIDRIGCPPYTPAMQKNIKSGQILKPCPIDDILPELKT
                     AIRKHPAVVLQAPPGSGKTTRVPLALLDIIEPEQGRILMLEPRRIAAVSAARWMAGTL
                     GEEIGQTLGYSIRFDSRVSKKTRIEVMTEGILTRRILANPDLAGVAMVIFDEFHERSL
                     QADLALALCLDIRRALREDLKILVMSATLDGGPVSALLDGAAIISSEGKAYHVEERYC
                     GDNPDKHIAARVDEVVQRALRETPGDILVFLPGAGEIRTVGDAIAETLHGKAASGISL
                     HPLYGDLPFEEQERAILPASARKIVLATNIAETSLTIQGVGAVIDSGLTRRLEYDPGT
                     GMNRLVTVTVSQAAARQRQGRAGRLGPGVCYRLYSYHTFQSLIPFAPPEIAISDLSSL
                     VLDLAAWGVKDPALLSWL"
BASE COUNT         5886 a         7660 c         6767 g         6847 t
ORIGIN      
        1 caagaaattg ggaggtatgt tcatggacaa aaggactatt ctggccatcg tcctgtcgct
       61 ggccgttctt ttggtttatc aaatcttttt tgtcaagccg ccggtaccgc aaaaaggcgc
      121 atcttcacag acgcccgccg gacagattca gcaggataag gccgccgctc cgtcacccgc
      181 agcggccctg acaaaggctc ccgcagcccc cgcgccgaaa gcggcagccg gaccgctaac
      241 gccgccccgt gacatcaccg tggaaaccga tcattacacg gccgttttct ccacacgcgg
      301 cgcggccctg aaatcactga agctcaaagg ctattatcgg gactgccatg aatgcaccga
      361 ggacatctgg ccgaaaatca aaggactggt gacaggccaa aaagcctccc cgaagcccaa
      421 aaccccggag tttgttgaac tggtcgatgt tcgcgaaggc atgccctatc cgctggcggt
      481 gaccttcccc gaatccggcg tcgatgtcgc accggacgaa gtttttgacg cggccggaaa
      541 ccgcatggat ttgaccggca ctcagcagaa acagcaactg gttttttcca gatcccaggg
      601 cggcgtcaaa atcgaaaaga tctattcgtt tgatccgtcc ggatacaccc tgggcctgga
      661 cgtcaaggtt cacaatctca ccggagcgcc catcacccag attccgcagt tgaactggta
      721 ccagtatgtc gatcctcaga agatcgacga cagctacgga catgaaggcc ccatcgtctc
      781 ggtcggcggc agcatcgagc tggaggaagt caaaaagctc cagagccagt ccgtacaggg
      841 tccgaacgtg ttgtggggag cgtttgaaag caagtatttc atcgcgtcgt tcattccgga
      901 aaacccgtct ctgaccaacg ccgtcctgac gcgcgacgcg gccaacatgg tcggcgtcgg
      961 catgaagggc cgcaaggaaa tcattcccgg cggtcagaac agcgtttttt cctactctct
     1021 gtacctcggc cccaaacaat acgacctgct caagaacctg ggcgtcggtc ttgaaaacgc
     1081 cattgatttc ggttggttca aatggctggc catcccggcc ctttacatcc tcaacttcct
     1141 ctacggattc gtcctcaact acggcattgc catcatcatc ctgaccacga tcatcaaaat
     1201 cgtattctgg ccgctgggca acatgagcta caagtccatg aacgagatga aaaagctcca
     1261 gccgaaaatc gaggccctga agcaaaaata caaaaacgat cagaccaaaa tcggccagga
     1321 aacgatggcg ctttaccggg agcacaaggt caatcccttt tccggatgcc tgcccatcct
     1381 gatccagatc cccgtgttct tcggactgta caaggcgctg atgtattcca tcgagctgcg
     1441 ccattcgccc ttcttcttct ggattcagga tttgtcggca aaggacccct actacatcac
     1501 cccgatcatc atgggcgcca cgcagttcat cactcagaaa atgacgcccg ccatgggcga
     1561 tccgacgcag cagaaaatca tgctgttcat gccggtgatc tttaccttct tctttttgaa
     1621 tttcccgtcg ggactggtga tctactggct gtggaacaat attttgcaaa tcggacagca
     1681 atactacatc aataaaaagc tggcgaccgg ataggatcga cggagttcaa aatgagcgca
     1741 aagatactgg acatagaaga aaaaaacatt gacgcagcca tcgaacgggc ctgccgggaa
     1801 ttcggcgtcg ggcgcgaaaa actgaacatc gaaatcattt ccgaagggtc caacggcttt
     1861 ctgggaatcg gcgcgaagaa ggccaaaatc cgcgcctcga tcttttcctt cgatatggac
     1921 ttctccacgg aggactccgc ccggcagaag gaacggcccc aagcaaaatc cgacggcaaa
     1981 ccggaagaaa aaaccgaccg ccctgcggaa agaaaaccgg aatcgaggca agaacaccgg
     2041 caggaaaaac ggcacgaagc ccaaccggag cgccgggccg cgaggtccgc acccgcggag
     2101 tccagggcat ccgctccccg tccggcccgg gtgaaaccgg cccagccgcc cgcggcgccc
     2161 gaacaatccc ccccgcccgc cgtgccgcgg gttgaagcgg ttcccgccac aacgccggcg
     2221 gcgcttaagg ccagagacct cctggccgga atcctcggca gaatgacgtt tgaatgccag
     2281 gtgaccgccg ccgaaacgga tgaaacgatc atcctcagca tcgaggggga tgacggaggg
     2341 cttctgatcg gccgccgggg gcagaatctg gacgcgctgc agtacctcct gaacaagtcc
     2401 gtccataaat cggaagcaca gcgcaaaatg atcgtggtcg attccgaaga gtaccgcaaa
     2461 agaagagagg aatccctgct ggcgatggcc gagcggattc gggagaaggt gaaaaaaacc
     2521 cagaagcctc tctcgctgtc gcacatgaac gctcacgacc ggcggatcat ccatctggcg
     2581 ctgcaggaag acgaagagct catcaccaaa agccgcggcg aaggcgaata ccggaaggtc
     2641 atcgttctgc cggccaaaaa aggaaacagc ctccgcccgg gtaaaaacag aagccggaaa
     2701 gagccggccg gcgatagctt ttaatgaatg gtggatggtg gatggtgaat agtgaatggt
     2761 gacaaagaca gaaacgatcg cagccattgc cacgccttcg ggcacggcag gcgtgggcct
     2821 catccgggtc agcggccctc tggcccctga actggcgcag cggcttttcc gtcccgccca
     2881 ttccaactgc acctggcaaa gccaccattg ctatcacgga gacatcatcg cggctgacgg
     2941 caaaacggtt ctggacgaag tgctggccac gctgatgcga aaaccgcgtt ctttcaccgg
     3001 cgaagacgtt ctggaaattt cctgccacgg aaaccctttg attctgcaaa acattatgga
     3061 acagtttctg gcgctgggct gccgcccggc ccgccccggt gaattttccg aacgcgccta
     3121 tctgaacggc cgaatggatt tgtcgcaggc ggaagcgctg gcggccatga tttgcgcgca
     3181 gtcggcaaag gcgctgcagc ttgagctgtc gcagctaaaa ggatcgctgg ggaactcaat
     3241 tgacgaactg cgctcactct tgatcgacgg tctcgccaat ctggaagccg caatcgattt
     3301 taccgaggat gtttccgaca aagaaatccc cgccgtcccc gggcaaattc tccaggccgc
     3361 cgccggcgtg gagctgctcc tgtccaccta ccgacaggcc cggctcatca cgcagggcat
     3421 ccgcgtggtc atcgtcggca agcccaacac gggcaagtcc agcctgctca accggctggc
     3481 cggcaaaaaa aaagccatcg tcacggacat tcccggcacc acgcgggacc tgattacgga
     3541 aacgatcact ctggacggca tcagcatcca cctgaccgac accgccggca tccgtccgcc
     3601 cagagacgac attgaaaaag aaggcataag cctggttcgg cagcagatgg agcaggccga
     3661 tattctcctg ctgctctttg acggcagcca accgctgacg gacgaagatt accggatcct
     3721 ggagcaaagc aaaaaacgcg actgccaaat cctgacggtt gtcaacaagc gcgacctcgc
     3781 gcccgcctgg gacaaccggc ttcccggtca cccgcttcac ggcaggcgcc tgctcgatct
     3841 ttcggcaaaa ttcggcgacg gcgtggagga tctgaaaaaa gccctgagcg atttggccgg
     3901 cggcacggat gcccccgcgg aaggcggcat gatttcccgg ctgcgccaca aactggcgct
     3961 ggaaaaggcc cttgcctgcc tcaaggcggc ccaaaactgc ctcgcaacgg gccagtcttc
     4021 ggaatttgcc gcctttgagc tttccgaggc cctgaacgcg ctggatgaaa tcaccggcaa
     4081 aaaaatatcg gaggatgttc tttcccggat tttctccggt ttctgcatcg gcaaataaat
     4141 tgaaaagcag tcctctttat gctagttttg tgcgcgattc aaagattttc gcggcgccgt
     4201 tttgccgaac ctataaatta ttttccagag gtgcgtcatg aaaacatcac ctttcctctt
     4261 ttgcgtgctt tgcctgctct gtttttccgt ccctgacgcg ccggcccagt ccaaatgcgt
     4321 tcacgcggac ggcgaagcgc tgatcctcaa caacgaccgg ccctcggcaa aaatggaagc
     4381 cgtggcccgc gccaagtgga cggccatcga agacgtcgcc ggaacggaag taaaggccgg
     4441 cagcctcgtg caaaacttca ccctcgttga agacgtgatg aaaaccaagg cgggcggcgt
     4501 ggtgagaagc tatcaggtga ttgatgaatc cgtaaaggat gacgttctga acgtgaaaat
     4561 caacgcctgc gtcgagccgt cccaggcccg gaacgccgtt tcgcagctgg gtctgaacaa
     4621 ctccatcgcg cttttcattc ccgcgcggaa accggggcgg cgcggagatg aatttgaaga
     4681 aaagaacatt ctttccgaaa cgctcatcgg caaactgacc gagcagggct tcaacgtggt
     4741 ggacgtcgcg cccacgcacg cggtgaacgc cagagacgtc gaaagcgccg ttcgcaccgg
     4801 ccgaaccctg tccatgcgaa gcctgacgta caagttcctg tcgaatctga tcatcatcgg
     4861 gaaggtggac tacaccattt ccaccaaaaa aggcgaagac atcggctacg gcctctccat
     4921 gcccttcaac cacgtcaccg ttcgtctcaa ttaccggatt ctttccaaaa acaacaacac
     4981 cggcaacatg gaaattcttt ccacgggcgt ggaacaggcc agggggctcg ccaacagcgt
     5041 cgaggacgcg gcggcccagg ccatgcagga actggcggaa accctgaccc cctccctgct
     5101 agacaaggtc gggcaataca ttcagggcaa caccaaaaag attgccgtgc aggtggcggg
     5161 cgtggaggat ctggacgcgg tcctgaaagt caagggcatc cttcagaaca tcgtctgggt
     5221 cgcgggggtg gaagaaaagg aaatggggtc gtttgtggtc agctatccgg aaaacaccct
     5281 gtatctggcc aacagcatcc agcagaaggg aagctttaaa atcgtcaatt tctcgcccta
     5341 ctccctgacg ctggagtatt tgaagcaata aaaggaggcg gcgatgttca attcgcgcgg
     5401 aaggtacggt aattatcctg ttctggtttc ggcaattttg ctggtcaccc tcctgatgct
     5461 gtgcggctgc gccacgaaaa ttcgcgtcaa catgctgcag cccgcgcagt atcatgaagc
     5521 atcgctgacc aaagctgtcg ccgttttgcc cttcaccggc cccggcggac cggaattcgc
     5581 cgcggaaatc gaaggcgtgc tcgcgggaat cggcatcgac gataaaccgt atttcacgct
     5641 ggtggaccgc gcctccgtcg acaaaatcgt cagcgaaatg aaattttcac acagcggcct
     5701 ggtggatccg aaaacagccg taaagctggg caaaatgatc ggcgcccagg gaatctacac
     5761 gggcgtcgtc acgcagaaca atcatgacga cagcttctac tacgaaaaac ggtcaacctg
     5821 cgtccattat gagaaacgaa gggatgaaaa ggggcgaatc tatcagggcg cctgccttca
     5881 atggcgcaac tacaacgtcc gctgcatgaa acgcgtcgcc aacttcgccg tttcgccgaa
     5941 gctggtcgat gtggccaccg gcagaatcgt atactcccgc aatctgtccg ccgtgaccgc
     6001 ttccaacggc tgcgaagaca cgcggccggt tcaaagcgaa tccgtccttt tggaacaggc
     6061 caaagcctcc gtcaaaaatc agttccggcg cgatattgct cctttctatg tcacccggga
     6121 aatccgcctg atcgattcca cggacggcat cgaatcaaat gaggcaaaag accttctcaa
     6181 gcgcggcttg gaatacgcgg ataagggccg catggacagc gcctgcgaac tctggggaca
     6241 ggcgcgaaac cttaccccag gctcttacgc gcttttgtat aacctgggcg tctgcgcgga
     6301 aagccgcggg gatctggata ccgcgctgac tctttaccgg caatccgaca gggcgctggg
     6361 gaaaccggac gacgacatca cccttgcgct ttcccgcgtc ggcgaagcca tcaagaaccg
     6421 cggcaaactc caagaagcgc tgggcgtcaa ataacggact atttcccaac gccgaacatg
     6481 tagggtcttt tcatgttgaa cagcctgggc ctgccgtcca ccatccggaa aataaacgca
     6541 gccgaaccgt ttttattgat ccgctttgtg gttttgacgt ccgtgtaact tctcgtgaag
     6601 ctaaccgcga cggatatgtt ctcgttgcgg gaatcggttg tcacgttgtt caatgtgtag
     6661 cggatgtcca tatcgagaaa tttgctgaaa tcccttctga tgcgcctctc cagaagcgaa
     6721 tcatccccca tataattctc ggcgacaaaa gacatgaatt gcctggcatt cttcccggtg
     6781 taggcctcaa ccatgccgtc gagcgtttcc cgcacagcct tttgcgtctc cagaaaagac
     6841 gtctttccgg acgctttctt gccgacggac aagttttttg acgtttcctg cgccatggcg
     6901 tccgctccgg aaaccgtcat aaccagcaac aagacaaccg gcaaaatcct cttgaaaata
     6961 ttcatacacg actcctttat tccgtattga tcattactgt acataccaga atcttccgcc
     7021 cagcgttctc gaaatctttg cttttcctga tttttcaatg gtcacttctt cgttgatgct
     7081 tgatttttcc tcatgcctca gattgcgttt gtacatcctg ctggtaatgg tgacatcata
     7141 agaaaccaga tagcgcaaac ggccgtttcc ggattcaaca agaatcatcg acaggttctg
     7201 aatattgtat ttgacttcat caaacatttt aaaagtcctg cggaggttca tctgcaggtc
     7261 ggacagcgtt gcgccgtcgt tcgcctgcca ctgatcgctg atcatgctca atacctgaga
     7321 atcgttgcgc tcctcatagg cctgcttgaa tcgattataa aaagcctgta tttcttcctt
     7381 atctctgccg gcagacacag ccggcgacgg cgcaacttcc gcggttttcg ggctctcctt
     7441 tttagcctgt tcctgctgcc cgttcttttc cgccgctttc gcccgttccg caatcgtgta
     7501 ttcctgacct tttcggatcg ccacggaaag ctgcttcctt aaatccagat aagcaagacc
     7561 caggggaaag ttaagtttag actcgtccac aaagatcagg cggccttccc agggaagcgg
     7621 cacataagat atcttcatgc tcttggcgtt tttttcacgg gctttatcct ccgcctcgtt
     7681 ggagcgctcc agcgtcacgg gcaaaaccgt gctgacctct ttgagcacct ccccataggc
     7741 aacagcgccc atatccggct tcaggctcgc caaaaggtca ttggccctgc tgatctgact
     7801 cttgtaaatg gctatatttc cggcgattgt gttaaagcca ataatgaaat tatcttcctc
     7861 ggccagacga atcttaccca ggagaaggtc tgcgtcaaaa gcggccaggc tggaaggcat
     7921 atacttctgc acaaagtcat agatcttgag ctgcttcgcc tccgcggcgt ataaggccgc
     7981 ttcacccttg gtcaggtagt gaggataagg actggacaac aggtgcaatc ccgggctgta
     8041 actgaaatag ggaattccct ccttctgctt ttgggcaacc catttactga actcggcggt
     8101 gatcgaggaa agccaggcgg catcggtgga aatgttgtga aattccgttc ccgccagagt
     8161 ccggtattcc cccagtttcg tcttcgcgga atagacaagc gccggaacac gattagccgc
     8221 cctgtcgtag cgatcaaaat catcccggct gaaggtcttg tctacccaca tcgtaatcat
     8281 gtcataatcc tgttttttga gatcaacggt cggataaaag gcctgccagt cacgaacggc
     8341 aaaattccat ccttcttcag cctcgatgat tttcgcttcc cagctatcca cgcttccggg
     8401 aagggggcgc gcgcgttccc tcaggctcag cgcgcgttca tcgatgacaa ccgaactgga
     8461 tgatattccc cctccgccca tccactcttt tgtcactccc ggccaactgt ttttctttaa
     8521 atcagcaatc gccctgtcca gcccttccac cacccggggt ttttcctcca gaggaagccg
     8581 tccatcaaac ttggccaggt ctttgcgcag ggtttccagc tgtcttttct tctcctgctg
     8641 aagctcgaca atgctccgga aagcctcgtc catggccacc ttcgtctccc gatagagact
     8701 ctcatcacct tccagaagat agccaacggc ctgctttgcg cggctgaagc cggcatcgag
     8761 tacttcaatc tgcaaccgga gcggacggcc actgctctcc tgacaaaagc tgtaggcatt
     8821 agaagccctg ctctcaatat cctggcaggg ctgtttgtcc ttttcggttt ttgcagccgc
     8881 ataacaggca tctctgggcc cgaaagacgc ccgataggca gcgtcacagc tgtcgtaggc
     8941 agcctggtat tgtttagcca gagggttgcg gggcttatta tacaggtcgg ttactttcgt
     9001 cagaacatcc gagtagtgaa cgacgtaggt ctggccggga ccccgatcat atccgtaata
     9061 agtctttccc gtccatcggg catatacgtc atcggcctga tcccactttc ctttgctcag
     9121 gctgacgaaa acgctgcgtt ttgcggtgtc aggttcctcg gaccaggcgt atggaacaac
     9181 aaaagccttg aaataatagg catatccgtc ttgtactgtt tcatcctttc ttgcatcagt
     9241 cggccgcgga gtcggttttt ccatttcctc cttctgtttc tgtgccacaa cgggaatctt
     9301 ttctttggct tgaatgtagc aggttttaag aaggccgaga agcctcgccc ggtctttggc
     9361 atagaacccc tttgccggga tgaactggat cacgttttcc gcaaagccct tgctctgttc
     9421 catcagatct tcaagcgacc gctggctctt gatgttctcc gcgttctgga tgtagccggg
     9481 acattttttt ccaaattctt caacggccgg cagcctctcc agagattgag cgaattttac
     9541 catgatcgcc tccagatcac gggaccttcc gatccggtct ttcagatcga taaaggactt
     9601 acggacctgc gtcaactgtt cttcgacctt ccttttctgg ttgaggtctc cgagcagact
     9661 ggcaatgtag ccctccatct cccttctcct cgatgcaagt aattccggtg tcggcccggc
     9721 aacaggttgg gcttgcaaaa acggcggcgg tggcgttgcc gttattgttt gcactactgt
     9781 atttggcgca gaaggtagtt tttgcggggg atgtgcagcc gcataccagc ccagccagta
     9841 agggccctcc ggccaatctc tttgcagcag ttgagtcaca taaacataga actgcgcccg
     9901 cacattctca tcatccacaa tatctctcca gaggagatcg atgctggcgc gggtcacggg
     9961 catcggatcg ccttcgccca ggaccctgcc ggggtttctc agccgcggat tattggcaat
    10021 agattcgaaa tggccgtaga gcgattccag ttgcaccgcg cgggtcgatt cccgcaccgc
    10081 cctcacagag gctacataga ccttcaccga cactacgaag acgttcaccg ttaatgagct
    10141 gagtcccaat gcgcctaaca atttgccccc gctttccgag ccggctatct cctggattgc
    10201 tctgagagcc agggcaagtt gcgcatcgtc atattccccc ctattgagct tgtccacaac
    10261 gtccagagcg gtagaaaacc gggccatggt cagccgggcc caatcaaagt tcttaaattt
    10321 atcaggatca agattaggct ggtctaattt aaggactgtt ccatagccgc tgttggtgcc
    10381 tttttcgacg aagttaccga aaaaatcaac tcctgcttca aaatcggtac taaacgcccc
    10441 tgcactattc accaacataa gcgtggccag aatcaccagc aggaataatc tcccccgctt
    10501 ataaatcaac ttccagtctc ttcttacgct catgatcagc acatcccgga acaatctgtt
    10561 tatattaact tttttactgg aaaatcattg cacataccag aatcttccgc ccagcgttct
    10621 cgaaatcttt gcttttcctg atttttcaat ggtcacttct tcgttgatgc ttgatttttc
    10681 ctcatgcctc agattgcgtt tgtaaatccg gctcgtaatg gtgacgtcat acgaaacact
    10741 gtaacgatct ccttgcaggg gagtgaccga cagattctgc acgttgtaac ggatctcgtc
    10801 gaatgtttta aacgtgcggc gcagcgtcat ctgcagatcg gaaagcgtgg ttccgtcgcc
    10861 cgcctgccac tggtcgctga tcatgctgat cacccgcgaa tcatttcttt cttcgtaggc
    10921 ttccttgaag cggacataaa aatccttgac cagggaaaga ttttcctggg ccctcttctt
    10981 ttcttcttcg attgccctgg cggctctttg cgcctcttct tccgcctgct tcttttcctt
    11041 ctcgaggcgg gctttttccg ccgcttccca ctggcgcctc tgttcttcag cgcgccggtc
    11101 ggcttcttcc tgctgttttt tccatttttc ataagccgga gcatagccca gaatcaggtt
    11161 gtccagattg acggcgacgg agttgttcgg cggctgaaga gtggtcctgg tcgcatcgaa
    11221 tttgcttctg atatagtctt ggagccagtt gtatgcattg gaaaaacgcc tgtattcctc
    11281 cgccaccata tcccgcgaat acttgggcat cttgccccag gtttcgaaaa caggctgggc
    11341 ttccttttgc gcctgttcct gaccgccggc aagtttgtca tgttcgggct tggccagctt
    11401 gtcgaagcgt tcgctaacct cgcgcagttt tttccagttt tcgatcaggg ggtccatcgt
    11461 ttgtgcggga accgccataa accatccatt ggaccttgcc tgaacgtagt ttttggcatc
    11521 cttgttcagc ttattcagca ggagcgtttg cccggcgtaa agtttctgcc atattccaag
    11581 gttctcccag gattgaaact ttccccaggg aagtgacgcg aaatctccct tggatacata
    11641 cttttccgca tcctgggcaa tggttgacca gggcggttta ccaaattcct tcgacagaac
    11701 ctccggaacc gttcctcccc ggtcggaata aaagtactga cccgtctttt ccacgctgat
    11761 tctgtctaaa gcgccgactg ccgtcgatgc gcgatggcga tacgtatcga cccagtccgt
    11821 gtaaaacgtc agttcccggg ctttgttctc gtattgtgcg gcaatccccg gaatatccaa
    11881 tttcagggat tttttaagat gttccggttc gtaaaaagtg aattttttct taacatattc
    11941 ctccagcgac gtgatatttt tttcctggtc cggagcacgc ggcgtaaatt ccattggatc
    12001 accaagccag tctatggaaa agttggatgc ccagtaataa ttaatcagcc gtccataacg
    12061 ggtgaggata ccattcaatt cactttgcgc cttgttggcc gtctggatgc gctcctggtt
    12121 ggcgctctca tactttttgg ccgcttcctg aacggtttta tgcaaatccc tcaaatcctt
    12181 tgccgtggac aacagctcgg cccggacatc cgcaatattt cgcccccagt aaagagcgct
    12241 ctgaatctca tcatccaatc tttcagcccg gcttttccac tgatcaatcc agtagggaac
    12301 aggcgccacg gcatcgtaaa ctttggcatt ggtttcgtca aatttggtct cccaaaggcg
    12361 ggattggttc cagaaatccc gggtgttttt ctttgcggca ttgtccactc gctcccggaa
    12421 aggatagaaa gcgtcccgga cccggtaatg aatctgatcg atttttatca gctcacggta
    12481 gggattgatc atcgcctgac cgaagggccg gagcgccgca taggcggctc tgacagcggc
    12541 attataccgg tctcccgctg cccgcagcga ggcgtagcag cggtcactcc cgtcacggca
    12601 atcgacatag atacgccagg cgcgatcata ttccgccgag gccgcgtcat acgacctcca
    12661 gaaaacctgg gtggcctgat tgtgcgcctc tctcttctga tcaagctctt ttttgatgtt
    12721 cttgtgccga tcgtgcaaac gattgtgcca gtccgtgacc atgatctgga aattatccgg
    12781 gtcgcctccg tcttccaccg cctgggcgta agatctcatc tgcgaaatca tcgcgttcac
    12841 ttcaccgctg tactcaccgg aagaaatggg cggaatggga tccatgccgt agacccatcc
    12901 gtttggcttc agatccggct tgggcgcgtc cgccattttc cgaacttctt cgtccagctt
    12961 ttgctttctg gcatcaaagg cgctcttgta ttgattattt aaggcctcca tggcggagtt
    13021 gaccctggcc ttatattcag tcattttctt ttcgtgcttt tccagggtct gttcgatctg
    13081 acgcatttcc tgctcgccgt cgcggagcgt tttcatatta tcttcatgga tttttttctt
    13141 ttcctgcagg acgtccctta gttccgtgta caccttcagg cgttctttca aactttttgc
    13201 attgtagtat gatttggtga ccatactgat gcctttagcc gctgctgtgc cggcaagcgc
    13261 aacccagccg accggcccgg aatataaggc aagaaccgca attgtggcct ctcctccaaa
    13321 ctcccagaaa tcatccggca aaggatttag caccatgaca gcgtcgatgg cagagcctgc
    13381 ctgcgcgttg ccgataagtt cagtaacctt ggcaatcttt gcgtttatac tcatcagttt
    13441 gtcgttttcg gccttccact gttcaccgag ctgtttcctc ttgtccgtcg ccattcgaag
    13501 gttgccttca aacgtccgcc ccagggagat gctcggcacg tattgatcag gattctgcgc
    13561 aagcacaatc gccgcattcc ggctgatttc aggcagattg gcgtcaatgg accggcccag
    13621 ctccgccatc tttgcgtcca gatcgttcca tttttgcata tccccgtgtt tggactgcat
    13681 ggcctccatg agcacctgac ccacggcggc ttcattcatg cggatttccg aggcaagatt
    13741 ttcaagagcg ttggccgcct gctgatactc ctgggcatca gcagccaggg gattgaagca
    13801 gcagagaaac agcagcaaca gagagcggca aatattggaa atgagtaatc gtgacatcat
    13861 gagaacctcc gattaaagcg gccagacgga ttgtccaagg agcaaactca acgtcttttc
    13921 tccgtccgcg gcgggcaggg acgcccgcca caaaaagaca tatatttttc ctccggaggg
    13981 aacggcgacg gacacggccg actccattcc cggctgaagc gtctcactgg tggaaagaac
    14041 ggcttttttt gcgcccttca gctgggcaaa atctccctgg gtgtgaatgg gcatcatgcc
    14101 ggacggcatg tttttcgcgg attccggatc tttcttagcc agttcctgcc gcattttttt
    14161 taatttttcc atttgtttca gattttccgc actaaacggc tctttcggca tggaatccct
    14221 cgcggatgtc agcgccagag cggcggggct tccggcggat ttcacggaaa cggtcacacg
    14281 ctccccgctc tgaaggttga agatgccgtc ttgatccgca gaccaccctt cgggaaggaa
    14341 aatggtcagc ccgcctgcaa cggcgtttcc ggcccgaagc ctttttgccg catccgccgc
    14401 ttcctgattg cctttcatct ttttttccgc ctgaagatag gcgttcaggg cgtcggccgg
    14461 ctttttggat ttttcataaa cggagccaag catcatcagc gcccggccgt catccggctt
    14521 caatttcaga tattcctgca gagcggccgt tgcgggggct tcatctcctg ctttgtgaaa
    14581 aagctcaccg gcctgcagaa gaaactgagg ccggtcggga tattgagcaa aagctttcct
    14641 tgcctcagcc gccgcggttc gcaaataacc ggccttctcc agcatccgca gccggttggc
    14701 ataactctcc gcagacttgt ccgccctgaa aacggcttca tattcctcca gggccatatt
    14761 ccactgaccc tgcttttcgt agagggcggc cagctccttt ctttctttcc atgcatcggg
    14821 tttcagcatc attccccgct ggtaaagcgc aacggcagaa acaggatcgg ttgcggccag
    14881 tttccgggct tcctcgaaaa aggccgccgc gtcccggggt gcggcaacgc caaaagatgc
    14941 ggggggggcc ggcttctttt ccggctccgc tttacccacg gcgggagccg gcaaaacagc
    15001 agccttggga gcttcctgcc gctgggcagc ctttgccttt ttgggagcag gtccctttgc
    15061 aaggcactgc ccggaaaagc ataaaacaat cgtcatgacg atcagaagac atacgggttt
    15121 aatcccgaag aactgaatct ttcttcgcat tttatcaacc tttcttcctg gaacaaccgg
    15181 ttgtcacccg gcaagaatcc ctttacggca gaaactgccg tgcgccgctg ggctgatatt
    15241 tgtattcaaa tctcatcgtg attctcatcg caggcggctg cggcggcggg cccggatttc
    15301 cgggaatcca ttcctccgtc acggatttga cttccatcag gccatatttg ccgttggcca
    15361 gtttaaaggc gtatgtgtgc ccctcttcaa accgtacccc catgccggcg ggatttgcat
    15421 atcccgttgc aggagcttca ttgacgctgc cgatactttt tgcgccaaga tccacaaagg
    15481 ttacactggg ctcaacaaat ccgtaaccaa atcccggacc gccgcccgta atcataaaat
    15541 ctcccgcagc agtcataacc tcaccattga caaaagaaaa ccccatggga ggatggccta
    15601 acgaaatgat ggttcttaag cccttttcca ccgtttcgct gggattactg ccgccgctgg
    15661 tctgctgtcc gttcttgtcc accgttatcg ttttatcgga agtttttatg gttgaagttc
    15721 cgtccgggtt gtttgtgatc ctgacgctgt catccataac aatctggtta aaagtctgcg
    15781 ccggcaccaa ggccacgttt cctctgccgt cggcgatgat gacaggctcg tttgttgtgg
    15841 acgcaacggt acccgtcgcc acgttcggcg cgccggtgat accgaaaatc agcggatttt
    15901 tcatgctgta aatccttgcg ctcttgtcgc ccattttaaa gacgaattcc gtaacgccct
    15961 tgtctttgac ggattgtccg gttttggtcg acatcagcaa cctgcggtag ctcaaagaaa
    16021 cagagatgcc cttgctgtcg gacgtgatgt tgttcagggt atagttcaaa tcaatatcgt
    16081 caaaggaact gaaatctttt cgtatggcac ggtccagatt ggtcatgtct ccggcgaaat
    16141 cctcgctgac cagcgacatg aaagtttttg cgtcatggtt ttcataggcg gcaatcaatt
    16201 tgtcgagcgc ttccctgata aagctcatga tattgcgttc ggaaagagtc agttgacgag
    16261 cggtagccgc aacgtcgttg gtcttgccca gcgtgtcgga aacttcgaca aagatagaat
    16321 aggttttccc cgtttccggc gagaagctgt actcaaaagc gccgccttca gaaaggttgg
    16381 cgtccttcca tgtttctttc ccgtccaggc tgacccgggc gctgccgatt ttcccttttc
    16441 ccgcgacggc ctttccgctg atggtgattt tgccgtccgg aagcatctcc ttgaacaggg
    16501 ttatttttgc gcctgtttca tcgaaagatg atttgttgac aaaaaggtag ctgatcgaca
    16561 cctcttcatc cgactcaccg aaaatccacc attttgcctg agcgcctgca aaagaaagca
    16621 gcacaagcgc gaaagacatt accattatca gacttttgct tttgtatttc atgattttcc
    16681 tcctttggaa aactttttaa atctcaagaa tcaaacctgc ggtcaggctg gtttcgcgaa
    16741 agttgtttcc ggcgttcgtg taactgaaat cgttgtattt agccctgaaa aacagcatgc
    16801 cctgattgag ctttttgaga aacggcgggc ggtaattgag gttgaaagag gcaaacgtct
    16861 tgtccgtgtc ctggccgacg cttttatcca gacggttctg gccggccttg atggtggagg
    16921 tgagcttcca atccggaaca tccagaccga atcccgcgga atactcataa atctggtcgg
    16981 tgctgaccac cagctcactg cgctgcgtcc acccgcccag atacacggca ggcttcagga
    17041 caaccatgcc caaagtgtgg cggctgctca aagaggtatt gtaggtgaac tcattgttcc
    17101 gttcctcgac ggaaatttta ttgtcataaa gcgtgtagcc caggtttgtg tcgctgtcca
    17161 gaaatccgaa gaactggtcc tgatagttca aggtaacgat atgattataa ttttccgtat
    17221 tattattgga tgtgacatcc agtttgtagg cgatgtcggc tgtaccgtat gcccttccca
    17281 gaaacctttt caaggtaaag ccgatctccg gtttataata gtccgtccgc cccgctgttt
    17341 tctgcccgtc caggttgtct ctaaaccaga ggaatcctaa attggccgaa atattttttg
    17401 tgtacttgta acgccatttt gccttggcct tttctctgtc cggcgttgcc gaaccgacca
    17461 gcgttgtgaa attgggcgat attctttcgt attccagcgt aacccggctg ggatccgcat
    17521 ctcccacagc cgtcaatctg taggccgcgc cgtggcgggt ggtctcgctg ccgtcatttc
    17581 ccgtccattc cttctgatcg gaaagagagg cttctccgga aacggtcagg ccgggaatcg
    17641 gtttgtattc catgtcaaaa gtataggcgc ggttcagata cagggaatcc gtgggattga
    17701 cgcggtcatt atcctgcgat tgaacataac ttaagcccgc ccagaaattc tgccccagat
    17761 tttccttgag gcgcgcgccc atcacttgcc gggcgacagc tttcaccggg ccgtcgtaaa
    17821 tcgcaaaggc attgtcccat cgcgaatagg catagccata aaccaggctc acttccggaa
    17881 tataagagtt ttccttaaca aaccggtagg aggccccttt tacggccgaa ctcagcgcgt
    17941 actgggaaaa tgtctcgaag gtgtcgccaa ggttcaaggt gtgaaccttg ttggtgattc
    18001 tcccctgaag gttggtgatg gaagcggttt gctggtcgtt ggtcgggtca tccgtcagtt
    18061 tgagcccgaa attgaaattg taatcaaacc ctttatagtt tccgagatcg ttgatattca
    18121 gaagattcag gtagctgaaa ccctgccgca gggatgactg gctgctgccg ggaccgctga
    18181 tgtcattata cgtgtagctg aattcattgg acgcatgaaa ttcgttttcc gctttcagaa
    18241 gaaaaggatt ccccagaaaa aagaaacaca tgaggatcac cgttagccac cgaaagcttt
    18301 gggaagaaaa gcacactcct gtgattgaag aggttttcat ttttttaccg ttcctttcat
    18361 aatagatatt cttctcaaat tcatttgatg tatcctgcgg cttcaatctg tcatgcctgt
    18421 ttttatatga taacttttta tgctccacaa gcgcttttta ggtttgcggg accggcttta
    18481 tttgacatac cagaaccttc cccccagcgt ttttgatatt ttgggcttgc ccgaactccc
    18541 gatggtcact tcttcctgaa cgctcgattt ttcttcgtgc ttgaggttcc ttttgtaaat
    18601 tctgctggtg atgactgcgt cgtagctgac acggaaccgg tcctccgcga tgttgctgat
    18661 ctgcatattt tcgattctaa actgaatttg atcaaaggtg cgaaatgtcc gccggagatt
    18721 ctgctggaga tccgaaaggg ttccgccgtc actggattcc cactgatcgc tcaggcagta
    18781 gatcacgccc gaggcatttt tcgcctcata agcttcttta aaccggccat aaagatcttt
    18841 aactttatcc ttgaggttat catccatccc ggacctgccg cccatatttc ttgaggcagc
    18901 ggaggctgaa aatccgacag gttcgccttt tgcaggaccg gtttggtccg ggcggaaggc
    18961 aggcgctttt gcgcccgatc ccggcggctg aggcattacc gctcccctgc ccgcaggcgg
    19021 cgctcctgta tttattccgg ccggcccgcc cgccgcaatc gcacccggac ccgcggcttg
    19081 tcccgactgg cccggaaaac cgcccgtgcc taatcccgtg cctccaggaa tggttttgca
    19141 ttcgccgttt gaataattcc cccccttggc gagacattcg ttctggagtt tctgctccgc
    19201 ctcggctttg gctttatcga tttcccattg tttggcctcc tgggcaacct gggcgttgta
    19261 gcgcgacaaa tgctcttcta tcgccttcac catgtcggag gatgccgtct tgatggtggc
    19321 gtttccgtgg ctcgccgcca gctctttggc tttgtcaagc gtcaccgaaa ccgtctgttc
    19381 gttcgaattc attttcatct ctatattcat cgcaaaggct gtgccgtttc caagcgctgc
    19441 gccgagcttc tcatcgaacg tgcggttttt gaggccttct ttataagtat tggttccgta
    19501 agtgcttgcg gggattgctt tcacgtcggc ggccatcctg gcaatccttg cggcatacac
    19561 cacattggcg ttcacgatgg cgtaatcggg cgtgtcgatg cagtaatttc ttatctccgc
    19621 ggccttcttc tccagcatga ccttgagccc cgtatccccg ttgggatccc atttggcgat
    19681 gccggtgcgc tcgacatcct tcagcaacgc atttgcggcg gcggcggctt tcgctttgtc
    19741 aaaaagactc aagtcctgcc atgagggcgc aaggtattta tgatggtttg aaccggcact
    19801 gttttccgtg gcgacggcta cttccaccac agtggcattg accgatcccg ccagcgtatt
    19861 tccccgaacc gcatcgcgcc aatagtcgat tctcttgaac agatagtcga gatattgcag
    19921 gtcgtcctct atgccggcaa gctgcttctg attttccgcg atcaagttgt tgtttaattc
    19981 agcttcaatt tcgagcgcca gcttttccac atttttcatt tcggcaattc ttttttcgat
    20041 gtccgggatt acctgcgcct tgggatcaga ctgcagttgc agggcataga ttctttcttt
    20101 tgcttccttg atctcctgct cataccggtc cttttctttg aggtatgttt tcagtttccc
    20161 gatgtattga atcgcctgac tgtgtttttc gctcaatcga tcaaaattct gtctctcgtt
    20221 ggatacgttt ctctcgatgc gccggacata ctcgtaaaca gccattccat cctcacccca
    20281 cttcgggaac ttcatcttcg gcaggcccag atatccgtcg gttttctggc ctctgaagct
    20341 gttgaggtac tgtttcgtgt ccgccgccgt tacctgggag tgctttgttt ccgtttccgg
    20401 ataaaagatc gggacgcttt gatccaccgc tcccagagtg tccagggcat ccttcttcag
    20461 cttttccata tcggcaaacc ttgccctctc ttcattggtc agcctgccga ttgctttcag
    20521 caaagcttca tgctgattaa aaatattgcc ggcatacagt tctcgtttgg cattgtaatt
    20581 ttttttatca gtttccagat cttccaatgg cttccatgcc gttttttccc aggcgcgttt
    20641 ggcatctgac gcactgttga agcatctctc ccaggtcttg ccgccatccg ccccacacgg
    20701 attggcgccg gaaagccaac ggcagtattg gtcacatgaa gcaatttgcg cttccagggc
    20761 ttttcgctga ttttccaatg tctgaagcat cgcatcgcgc cgggaattat aggcgggatg
    20821 ctgatcaccc gccggatttt ccttgttgtg gttctcctga aaggtcctgt attttttttc
    20881 tgtatcctcc ttgacaagaa tcacttcggc ttccatgtcc tgaagagact tcgccttgtg
    20941 cagtctcgcg gcaacctcgt caatgtcaat gaaatcagga gaaacctcgg ccagtgaaat
    21001 cgaggtcttg tattggggtg tcacggtttg cgcagtaaaa tcttctttaa tggttttggg
    21061 aatatccttg agcgtcttgg acacatcgac ccaggagttc ttgagggcgg cctttgtcgc
    21121 ctgcttcttc ttgccgatat ttcccgtgtc gggaagcact cttacataaa ctcttgcata
    21181 atacccgatt ttgtcccgaa catcgtagag gctgtcccag ttttgatttt tctttgcagc
    21241 ggcaatgatt gtgggaacct gctttgcata ggcatcgatc tcgggaagtt ttttttcagc
    21301 ctcctcggcg ccgacaacaa acttctggaa ttcgggcgtt atcttcttgg atgccttctc
    21361 gatcttcgcc aacgtctgcc gcaggatcac ttcgctctcg acagccctgg catatttatt
    21421 caactggctc agaagaccgg cgatccatgt ttccacctgc cgcttgtcct tgaatatttc
    21481 cgcctgctcg gccgtgtgag cgcttttcca gccgtctaca gcggcaatgc tgagccaatc
    21541 ccaggtcgag ggctctggaa aggtttctcc gagttcttcc gacacatagg acttgaacaa
    21601 attacgccat ctctcatcct gcgccacctt cctgaacatg tattccaccg actcattatt
    21661 gaccggaatc ggatcgctct tgtctccgag ctgcctgccc tttttgggct tcagcaccgg
    21721 gtcgctttca atccagccat atagagtttc tatgttgagg gcaatctttg tagccctgac
    21781 ctgagcaaag gactctttgg tgatggaata agccgtcaat gctacctgaa agacccccgt
    21841 agagagaccg acgtatttca ggaggccttg gccggacggc catccggcga tttcggacag
    21901 gacggccagc atggcttcct ctccggcgcc ggatacatcg cctgtggcaa aagcttttgt
    21961 cgtcacgctg ctgtaagtgg cggcaaaccc ggcggccttg ttgaacacat cgatggaagc
    22021 gccaaatctc tggccgtcct ttacctggtc aaggaaaacc ccgtagcctg tcgtaaaaag
    22081 agaggcgtcc gcgttgaaca tggcgtcata aacatcttct tctgaagcgg ctttgcaaag
    22141 cccgggcatg gccagcaggc aaaggatgaa aatgagtatg caaaacttca tcatgttttt
    22201 cttgatgtac cggcatggac caaaacagac tgttggattt ttcatagatt tgtctggttt
    22261 ccagaaaaaa ttgaattccg accaaggttc tttgaatctc cccgcctcac gatttgaagc
    22321 gcttctttgc tgtataataa gaaacttgcc gtgcaatttc cagcattatt tattgaacag
    22381 tccagaacct tccccccagc gtttttgata ttttgggctt gccggaactc ccgaaggtca
    22441 cttcttcctg aacgctcgat ttttcttcgt gcttgaggtt ccttttgtaa attctgctgg
    22501 tgatggtgac atcataggat acattgtaac gtccgtcctg accgggtgtt acggagagat
    22561 tctgaatgtt gtagcggatc tcgtcaaaaa cccgaaaggt gcgccgcaga ttcatctgca
    22621 gatcggaaag cgttgatccg tcgccggcct gccagtcgtc gctgatcatg gccacaatcc
    22681 gtgaatcgtt gcgcgactcg tattcctgtt tgaatttgtc gtagaaagcg cgaattttgc
    22741 cgacatcctc cggcgaaaca gcatggcctg ccggcgcgcg aagatcaacg gaatgcgtcc
    22801 agtccccgac cgtgtaggaa acatgcgttg ccttgccggc cgcaaccgga accatcgccg
    22861 tatagacagc ctcgggtatt tcggctgccg ttgaggtctt gatggtcagc ggcaggttct
    22921 tcccggctgc ggcaactctg acaagagcgc gggagatgcc ccagccactg gccgtgattt
    22981 gaaaaggcac tttgccgtca gtaatgtcac cggcggtcaa acggacggcg ccgtgaaggg
    23041 tttgcgctcc ggagccgatc ttcggctggc tgtaggtaat tttctcatta attttttgcg
    23101 aacgtttttg cataagcgga ttaaaatgct cactccaaaa agcggccagc ctgttcaaag
    23161 ccgcccttgc ttcctcggat aacacagcat ctattttcca gtttccagaa ttcagagaac
    23221 tgccaagatc cgactgatag gcgctgtagg ctccgctgaa gtctttgatt ctggcaatgg
    23281 ttgaggcctg ggctttgatg gaactcgccg cattttctcc ggttgccgaa gacacttcca
    23341 tagaaagctt tttcatatcg ccatccggca taatgatgaa tgccgcttca tcgggaattt
    23401 ttttcagggt ttcgccatag cgccgcgcgc tttccgtcac aacatagcgg ttggcctcgg
    23461 caatgtaagg aagataagcg gcaaggctga actttgaacc gctcaagtcc ggccggacca
    23521 ttcgggaaga cagggagctg ctttgatatc ccagcgtacc ttttgccctt tgaatcctgg
    23581 aagatctgga aagcgcttca actttttgac ttttgtctgc ggccttctgc atcagctgcg
    23641 cctgcttctg cagcagctga ttgtattcgg ccatatcttt gttgacctgg ggcagcaggg
    23701 tctccaattc cgcctggttc tttgccgcgg tgattttgga catgaccacg ttgatctgga
    23761 tcagtttctc ctgctcgtat tcacgacgtt cgttttcgat attttccaat tcctggtcgg
    23821 cctgggcata ttcctgatag gcgggctcta cctctttttc cagaagttct ctggctttct
    23881 tgctgccctg ttcaaaggcg cttttatact tgctttggag ggacctgtag ccggaaagat
    23941 tgttctgaac gacagtaatt tcatccacca gttcctgccg tttgagctcc acgcccttga
    24001 tctcattttc cagaatcagc gaataattgg cggtaaattt ctcactggat cggtaagtgc
    24061 cgcctgttcc atactgggtc tcgagattct gtttctgcgc atacagaccg gaaagcccct
    24121 gaaggctgga tatgcgcccg tcgtaaaccc gaatctccag ctgctgagac tgaagccatt
    24181 tttcggcgtt ggccacaagc ttttccaacc tttgggcctg ccgcccattg taatcaacgt
    24241 tgtcctcgtc aatggataca ttgccgggat cggtcttgag ctcttccaaa ttgccggatg
    24301 aaacatttat tttcagttcg tttcttctgg cggcgttttc tttctggatt tcctggaccc
    24361 ttgcattgat ggcgctgcgc ttgtcttgaa tctcctgcga gatctctcta gtcttatacc
    24421 aggctttgcg ataactctcc gcgcattttt catcgcactg cgttggcggc tcggccatgg
    24481 cgatcttttc ctgcgcggcc ttcttttgct gctcttttaa tgcctcctgg gcggcaaata
    24541 tttttccctg ctcctgttca aagacaagca gatttttctt tacataatcg gatgcatttt
    24601 tcatcgcttt ctgcatatcg cccacatcct caatggctcg gagaatggaa tagtgagcgg
    24661 acactgcctg acggatcttc tggtcggagc ggacaaaaga catgacggca taatcttcac
    24721 atcccgagtt ttcaaagctt ctccttttgg accagtcaaa cggcccggac acacagatgc
    24781 ttccgcactt ggaactgccg gccgcgcctc cctccttgca ataccaggca accagaccgt
    24841 aagcgccctg atcatcttcc gccatgcgcc ggcctattct tccggcgaaa ctttgctcct
    24901 ccgacaacgg acaggattct ttgcacttcg gcaggacgtc tctcatgaat tccttgcgga
    24961 tgtaatcaag attattttcc atgacggcgt ccgatacgga cgtaagaatt ttgtcccacg
    25021 cttcgagata tgaaaatatg tatttggaat aagggaatac tttggtgaat atttctttgg
    25081 tcgcgtcata ggtcgcgccc gccacgttgc cttccgccac cttgacgagc agcgaattca
    25141 ccgtggtgag cttttcatat gcgtcactgt atttgttgta atccttcatt aactccgaga
    25201 aatctttcct gccggagaat ttggcgatca ccggatattt ttcgtatgcc aaattgatca
    25261 attgcggcgc tttctgatca accttttcct gaaccttttt cttagccata tcttccgccg
    25321 ttgatgccgc ctgatccttt gccgttttct tcagatattc cccggttgtt ttcggactgc
    25381 ttttctccgc ggaatcctgg gcaaacacac attgggaaca ggcgagaaag gcgcaaatca
    25441 acagcaggca ggtcatcttt ttcataatct gtctccttaa agaacggtta ttgcctgcta
    25501 gcaactttcg agggtttttt caaacttttt attttcaaac tttttattgc ttttatccat
    25561 tgttttctat aatttacatc atcgccccgc caaaacgccg ggacaacagc aaggaaggat
    25621 gtaaatgtca aaaagaaaag cgaccgcaaa gaaaaataaa gggaccgctg gaaaggcggc
    25681 cgtaaaagcc gcggaaaata aaatcacgga cagggatcgc aaaatggcgc tggtctgcgt
    25741 gaaatgcccc gtgtgcagac atgcccgcaa aaaacaaaag ggcgccgcgt tctggtttgt
    25801 caaaaagatt gaaggcgggc tttgcccctt ctgcaaagcc catgaacgcg tttacggcag
    25861 gaaagcccac cagccgatct ggtaaaaata acagaatcat ccctttattt tcatcagtgg
    25921 aatcaaatcc atggtgatga ggcacgattg cattgacagg atcggctgcc ctccctatac
    25981 tcccgccatg caaaagaaca tcaagagcgg acaaatttta aaaccctgcc ccatcgacga
    26041 catccttccg gaattgaaaa cggctatccg taaacatccc gccgttgtcc ttcaggctcc
    26101 gcccggttcc ggcaagacca cacgggttcc cctggcgctt ctggatatca tcgaacctga
    26161 acagggacgc atactcatgc tggaaccccg gcggatcgcc gccgtctcgg ccgcccggtg
    26221 gatggccgga acactgggcg aggagattgg ccaaacgctg ggttattcca tccgctttga
    26281 cagccgggtc tcgaagaaga cccgcattga agtgatgacg gagggaatcc tgacgcggcg
    26341 cattctggcc aatcctgatc ttgcgggcgt cgccatggtg atttttgacg agttccacga
    26401 gcggagcctt caggcggacc tggcgcttgc tctttgtctc gatattcgcc gcgccctgcg
    26461 cgaagacctc aaaatcctcg taatgtccgc cacactcgac ggcgggccgg tttcagccct
    26521 tctggacggg gcagccatta tttcatcgga gggcaaggcc taccacgtgg aagagcgcta
    26581 ctgcggcgac aacccggata aacacattgc ggcgcgcgtg gacgaggttg tccaaagagc
    26641 gctcagggag acgccgggcg acatcctcgt ctttcttccg ggcgccgggg aaattcgcac
    26701 agtaggtgat gccatcgcag aaacgcttca tggcaaagcg gcaagcggca tttcacttca
    26761 tcccctctac ggcgatttgc cctttgaaga acaggaacgg gcgatccttc cagcctctgc
    26821 acgaaaaatt gtcctggcca caaacatcgc ggaaaccagc ctgaccattc agggcgtcgg
    26881 tgcggtgatt gacagcggat tgacccgcag gcttgagtac gatccgggaa ccggcatgaa
    26941 ccgtctggtc accgtcacgg tttcacaggc cgcagccagg cagcgtcagg gccgcgccgg
    27001 ccgtctgggt cccggcgttt gctatcgact atatagctat cataccttcc agtccctgat
    27061 tccctttgcc ccgccggaaa tcgccatttc cgatctgtca tcgctggttc tggatctggc
    27121 cgcatggggt gttaaagatc ctgctctgct ttcctggctc
//
LOCUS       DAOSIM010000003        24758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_2246_length_24758_cov_6.760758, whole genome shotgun sequence.
ACCESSION   DAOSIM010000003 DAOSIM010000000
VERSION     DAOSIM010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 24758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24758
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2246_length_24758_cov_6.760758"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..1746)
                     /locus_tag="PLR47_00220"
     CDS             complement(10..1746)
                     /locus_tag="PLR47_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOG11384.1"
                     /translation="MKKIMIGFCLLAALLMAATLTAKEKYTVTILPFALHSAENIEYV
                     RQGINDMLISRISGADKIDVTRKEVVSDVLKKTGGKELGLADVQDIGRQLNSDYVVWG
                     SITKIGKSISIDGKLIDITGGKSDVGIFSQSQTLDEVIPKINDFSQRIVRHITGGAAA
                     ESAPAAVSTAPAAAPPPVPGASREAQIIAGMKSGKGKGTLTSMINAEFINSSDPLDRK
                     KGFWMSQQFKTEFNGMDVGDVNRDGRNEVVIIDPHNVYIYLKTKDNLQLLKQIKGKSY
                     HKYISVDVADINKDGTPEIIVTSLNDRLLNSFVLQYEDGDYKTIASDIRYFLRVIIPS
                     SGAPVLLGQAYGLGSFHDGQGGRVFDTQIYEIVWRDGKYVEGDKQKIPVGLSIYGLTL
                     DTLGAGTSEKIIALDELDYLCIITPTTKPLSRIFSFGFSPDELIWRSDSQYGGSNNYI
                     DNVDKQKQQSSEATQEDSVFANLRIVMFDTNNDGKKEMIIVKNLSSVGRIFKNLKLFS
                     SSEIYNLEWDGLGMAENWRTKKINGYVADYCFKDVDNDGKPEIVLALVQSVGATLSER
                     SVLVVYELEMPQ"
     gene            1971..>3020
                     /locus_tag="PLR47_00225"
     CDS             1971..>3020
                     /locus_tag="PLR47_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOG11385.1"
                     /translation="MEKATLKLNGMTCELPIVEGTENEKGIDISRLRQDTGLITLDVG
                     YQNTGSCISSITYIDGDKGILRYRGIPIEELAEKASFTEVAYLLIYGELPNREQQQLF
                     SQRLTQNSMIHEEMLRFFDGFPPTAHPMAILATMVNSMSVYYTDYFTEDFRAETFDLM
                     AASLISRIRTIAAYSYKHSIGHPHVYPKRDLKYASNFLYMMFDSPVNPYVPDPDIERA
                     LSTLMILHADHEQNCSTSAVRLVGSSMANLYASICSGICALWGPLHGGANQKVIEMLE
                     EIKGSKLSIQSCIDSAKDKTSKFRLFGFGHRVYKNYDPRARILKQYCETIFKKHNYQD
                     PLINIAMELEEAALKD"
     assembly_gap    3021..3030
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3031..3241
                     /locus_tag="PLR47_00230"
     CDS             <3031..3241
                     /locus_tag="PLR47_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOG11386.1"
                     /translation="NVDFYSGILYRMIGIPTNMFTVMFAIGRLPGMIAQWKEMHDTVP
                     LKIGRPRQIYTGSNLRHYIPMDERG"
     gene            3514..4722
                     /locus_tag="PLR47_00235"
     CDS             3514..4722
                     /locus_tag="PLR47_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG11387.1"
                     /translation="MKTIGRILLVMGILAMTALTGQAVAQTKAIKVGAAINLTGPASS
                     WGQFHEKGQRDYFRYVNEEKGGVGGRKIEMITMDTAYKVPEAQAAVQKFILQDKVDMI
                     ATWGAGEGLAAKPIVQKHKVPTINYSTSWEILEKPVGYMYLPFGSYKMDCRAVLEYIK
                     TIHTGNGAPKVGLLTYNNAYGRSIHAPSREYAAKLGVQLVAIEEFPPRTVDLNTELLR
                     LQKKGAQYIFIQMLPSTIITAFKSADRIKYNPLFLGTWTSTDPDFFRMGRGLIRDRLV
                     MQFPGGLPSDKAKGMEVMKELWKRYKTVKSFDASYWEGVVVGMIMERAFLRAWEKSKV
                     INPETVNAAMESMKNEDFGGLVPAVSYSKTNHEGSFTARMVKVMENGTYIPLTNFYIP
                     GKDKIKVLKR"
     gene            4804..5610
                     /locus_tag="PLR47_00240"
     CDS             4804..5610
                     /locus_tag="PLR47_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG11388.1"
                     /translation="MKAFRSRPSIMINRPQEDKKAELEIRNLRLSFGGVVAVKDVDLS
                     VHTGELTAVIGPNGAGKTSLLNCITGFYRPQQGSIVFNGRDITHLHTHQLVGIGIGRT
                     FQNIELFPGMTVLANLTLARHIHCRYSFLHSCLFTPSVREEEIRHRRILEEIIRKQTV
                     GSLPYGMMKRVELGRALALEPRLLILDEPFAGMNLEEKEDMVRFLLELNGRWGLTMVL
                     VEHDMSIVMSISRHVMVLNFGEKLGEGAPADISANPEVIKAYLGDAQSLE"
     gene            5626..6999
                     /locus_tag="PLR47_00245"
     CDS             5626..6999
                     /locus_tag="PLR47_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG11389.1"
                     /translation="MDKATPHPPKPNQITLPQLLAANAGKYGDTRTAIREKAFGIWKK
                     YTWGEYFSFVKRTAAGFASLNLARGDNLCMIVNNSPEWLFSELAAHALGATSLNLFTS
                     SIAEELAFSIGRVRSPLVVVQDQEQVDKLLEVKDKLTQVRFVVYVDPTGMTSYEDNPW
                     LISFAALLERGERHMADQPDYVDAEIGKGRPRDIAVMMQTSGTTGVPKIAMLSHHNLA
                     AMAHQWVEAAHIRPEENWISMSPTAWIVDQMWGMGVALAGAMTMNFPETPETVLEDFR
                     DIGPSLLITASRFWEDMASRIRVKITDAGWLKRKLFYLGESVGREVVGKKLRRESISW
                     PLGLLYRFLTLAVYNPLLDRLGCSRFHSAFTGGHPISPDVIGFFRAIGLNLKQCYGLT
                     ESCGIFQIQPDDEVKLETVGKPLPNTSIKLAEDQEVLVKSEANFSGYYNDYQSTEGAT
                     STTTATC"
     gene            7140..7517
                     /locus_tag="PLR47_00250"
     CDS             7140..7517
                     /locus_tag="PLR47_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11390.1"
                     /translation="MINIDLGNVGSWAEERMIPYTTYMDLSQQKAVEEMILREVREVN
                     GQLPEVMKIKKFVLLYKLLDADDDELTRTGKVRRRFVYGLYIKIIEAMYAGAQNVPVT
                     GKIRYRDGRIGEIETIINIISVD"
     gene            7529..8407
                     /locus_tag="PLR47_00255"
     CDS             7529..8407
                     /locus_tag="PLR47_00255"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOG11391.1"
                     /translation="MEFFLQLLVNGVCIGLLYGISAMGFVMIFKASSVLNFAHGELLA
                     LGAFFFLSLVTWGKIPVAAAFLLTLIGCFVLGVLIEKFFLRPLIGEKLIFVIMLTVGL
                     AAMFKGLILLVWGGNLHTYPEFLPASLEFHLGSIYIAPVYSATLVISVVFLVLFGLFF
                     KKSSQGIFMRSVADNQKAALSLGVHVRRVFALSWAIAAMVACMSGIVLGIINGVNVHD
                     LSAIGLKVFPVVILGGLDSIGGAIIGGIIIGLLETFTGGYLSPSLRDVVPYIVLVFIL
                     MVKPYGLFGLVEIERV"
     gene            8409..9479
                     /locus_tag="PLR47_00260"
     CDS             8409..9479
                     /locus_tag="PLR47_00260"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOG11392.1"
                     /translation="MKKPSFHPCGNFRETYAEELAIFDTWFGRVSLIVFLLLLFCLAP
                     LMLDRYLLYILNSIGIMAIAAIGLNILIGYTGQISLGHGAFFGVGAYAGAILATRLNL
                     PFFVCIPAAGLITAMVGMIFGIPSGRLKGLYLTIATLAGQFIIEYVLIQWESLTKGTM
                     GITLPEPQVLGVNISGDKAFFFLIFACLVCMTWFAVNIMRSKYGRAFVAIRDNDRAAE
                     GMGIPIFRYKLLSFAISSFYAGFAGSLWAYYMVSITTEPFNLALSVEFIAMVIIGGMG
                     NIPGAIFGATFITVLNELLRFATGALMNVGWIAGLGLNMAPLREFVFGLAIVLFILLE
                     PKGLAELWRIVRSSFRLWPFSY"
     gene            9565..10365
                     /locus_tag="PLR47_00265"
     CDS             9565..10365
                     /locus_tag="PLR47_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG11393.1"
                     /translation="MGEILLQLNNISVVYSDVIQVLKGVSLSVANGHIVSLLGSNGAG
                     KTTTLKAISGLLKPENGEVTDGSIIFNGVNIQNQAPEFITRQGIIQVLEGRQPFKYLT
                     IEENLRVGTATRPGKPYKEDLEMVYSYFPALVKRKKALAGYCSGGELQMLVIGRALMA
                     HPQLLMLDEPSLGLAPLVVQEIFRIIRKINEEHGTTIVLVEQNAHMALQVAHYGYVME
                     NGKIVMEGTARELADNPDVKEFYLGMAASGIAKSYKDVKSYRRRKRWL"
     gene            10380..11633
                     /locus_tag="PLR47_00270"
     CDS             10380..11633
                     /locus_tag="PLR47_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG11394.1"
                     /translation="MKRLYDEKEAWSPEKRKAWQDRRLSRMVRLGYQKSPALRRMLDA
                     NKINPARIKTREDLEALPVTSREKLVEMEMRKPPYAGLENSTIAVDRIFTSPGPVYEP
                     HLSENDFLWARAFFAAGIGSRDIALNAFSYHLVAAGLTFHNGLRKVGATVVPSGTSSS
                     QIQVQLIRDLKVTAYVGTPSFLMSIIDQSKEMGYDFKKDFRVKKACFAAEPLLPSLRQ
                     RFEKEYGIRTFQMYGATEVGDVAYECPAKSGWHICEETIVEIVDPATGKNVAPGELGE
                     VVVTRLNDLFFLLRFGTGDLSRLVTKKCSCGRTSFRLAGIAGRVGDAVKVRGLFIAPS
                     QMKLLSAKFEGLPLQAVVSRKGHEDVLTLRIEKINSQVNTDSWKNNFKKIFKELCSVR
                     IDVLEVVEKGGIKTDQKVIVDSRTW"
     gene            complement(11590..11967)
                     /locus_tag="PLR47_00275"
     CDS             complement(11590..11967)
                     /locus_tag="PLR47_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11395.1"
                     /translation="MNDATRAALFSFLLIPGGGQIYLKRYARGMAFLIPAAAATLTLA
                     WMIFQVVLMIVRDAASEGAGTTRILRNAENALRTIDLNLALGLLILILFLWISSTIDA
                     YRLGKKTEPPTTSGNRRSPSDPF"
     gene            complement(11977..13716)
                     /gene="recJ"
                     /locus_tag="PLR47_00280"
     CDS             complement(11977..13716)
                     /gene="recJ"
                     /locus_tag="PLR47_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOG11396.1"
                     /translation="MNERGQLPVTQWKIKDSGPKTIEESLVREFGFHPIISQMILKRR
                     VGSLEDAHRYLHPSLNDLHSPFLMQDMKKGVARLLKAIYDGEEIVIYGDYDADGITSV
                     VILYKFMKQLTARVSYYIPDRVQEGYGLKIPVIDRFKQKNVKLIVTVDCGISDIEQIA
                     YAKSVGIDTIVLDHHEIADQLPPAVACINPNRSDCSFPFKHLAGVGIAFNFLIALRGT
                     LRREGFWKNDDYPNLKEFLDVVALGTIGDIAPLLDENRIFARIGLDLITEGRRPGIKA
                     LKEVSGIDGQLVDSFKASFCLIPRINAAGRIASPLDAVELLLAETMEEARPLAEKLDL
                     HNRRRQAMEKDILNDILEKLGAKPDLENISALVFSSEKWHPGVVGIVASRLVDLFSRP
                     TFVISLKDGVGKGSGRSVSDFNIHKGLQQCAPLLLSYGGHYRAAGISIKEDDIDEFAC
                     LLDEIIRNTVQSSEMVSQTLIDAECRLQDISLDLIRQMSMLAPFGCENPEPVLCSRNI
                     KASSPVIVGNNHLKMRVVDDGASHSAIWFGMGKYLGVINQASFDVVFSPQINYWNGSS
                     DVQLKIRDAAIVA"
     gene            13931..14656
                     /locus_tag="PLR47_00285"
     CDS             13931..14656
                     /locus_tag="PLR47_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11397.1"
                     /translation="MDPDIYIYNIAPDLLTIHPDINPRQTHSIPRFIEAPPDHLRTAY
                     IMFHLLVDDLAHYGSISMTCKEGFDPDSSGYTYLKGRNLVRSIMELHAIIGKDISYSE
                     AAYRSHLIIEMIYDLIIGPQIDKNGSIDLLEEAIHFTFDRREKEFCSDISWLYGIDFE
                     QSREVLKIAVSYITGERLRRIMNIEGRIRLFTDKFGLRNNNEMFAEAIAALFQDALDS
                     IENDDFLQETASTIRSCGWLPTR"
     gene            complement(14653..14850)
                     /gene="rpmB"
                     /locus_tag="PLR47_00290"
     CDS             complement(14653..14850)
                     /gene="rpmB"
                     /locus_tag="PLR47_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOG11398.1"
                     /translation="MSRICEVCGKKPAIGNNVSHANNKTRVIWRPNLQKLRCVDEKTG
                     AVKRVKVCTRCIRSGFVKKAS"
     gene            complement(15014..16312)
                     /locus_tag="PLR47_00295"
     CDS             complement(15014..16312)
                     /locus_tag="PLR47_00295"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOG11399.1"
                     /translation="MQDDFAISVLGVCAIDSPLAINGFVSDDDRILFNTRLKDCDALM
                     VSTGKPLSLEPAGPRRKIFFDPAGTKAAIVTCGGLCPGINDVIRSVTMTLFYRYGVKN
                     VLGIKYGLRGLNPEYGKIPIQLTPDFVKDITHIGGSILSSSRGPQDVKVMVDYLELLK
                     IDILFCVGGDGTMRAAEKITEEIHARKDPIAVIGIPKTIDNDLNLIEKSFGFDTAIAK
                     TVEAVGSAHVEAKGAYNGIGLVKIMGRLSGYITATTALAQGDANFVLIPEVPFDLEGE
                     KGFLKALENRMKASGHAVILVAEGAGQELMQQDEKKRETDASGNVRLQDIGLFLKSAI
                     ETHFREAHVEINLKYIEPSYMIRSVPANASDSLYCSSLGQYSVHAAMAGKTGLLVALR
                     RDEYVHLPLSAAISGRQIDPDGNLWFRVLESTGQPAQMKN"
     gene            complement(16317..17759)
                     /locus_tag="PLR47_00300"
     CDS             complement(16317..17759)
                     /locus_tag="PLR47_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11400.1"
                     /translation="MYDLLVLGDDLASHVAAACASRNGLKTLLVAPGGLGGLQLIGDF
                     VFHLDPAPMTGLAPGQPGFSVLAELGVEIPETHPTSMSPAYQVLLPGHRIDFSNDSEA
                     LLSELAREFPKRETDIRDFYGIALQASSVFQDWIAEHPRVQPQTLKEYFSYLKIYPFV
                     LQYKFAAARFDKMLSRDAALEKVWEAQQALLSQNTDDLFSFASAFQYSAPLRGVACFP
                     QGKQFLFNEMIRTLESQGGLYLSGHAVKSLDRGKTITLEMEAPDKSTSRVAGRYLIVS
                     TKSEALPLITQKRGHAFFWDKFRKAGIAGYPFTLFLGVAQKFLPEQMARHMAVVADVH
                     RDLHDRNLILLETGMPEKGKPLPQAKNFMTATVYLPADDAAWAPEALRREAESILERL
                     DQFFPFLKDGIEMYDLDKSIELSLKYRKVFSPKYRVRNAFFTSFSAKNDRTRWSNVFL
                     TGTSLLADAGFEAEILSGRNAVSRVLQKGK"
     gene            complement(17801..18460)
                     /gene="purN"
                     /locus_tag="PLR47_00305"
     CDS             complement(17801..18460)
                     /gene="purN"
                     /locus_tag="PLR47_00305"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOG11401.1"
                     /translation="MADLLKLGVLISGSGSNLQSIIDHIEKGALGARITMVASNRPDA
                     YGLTRAQQYRIPTAVVSHRDFENREAFDRELLRILQGAGVELVVLAGFMRILTPFFLR
                     AFPDRIINIHPALLPSFPGTHVQQKALDYGVRFSGCTVHFVDEGVDTGPIIIQAVVPV
                     FAGDTAQELAARILKEEHKIYPQAIRYFAQGRIVRNGRRVEIRDAGNHDAALSNPPPE
                     G"
     gene            complement(18470..19537)
                     /gene="purM"
                     /locus_tag="PLR47_00310"
     CDS             complement(18470..19537)
                     /gene="purM"
                     /locus_tag="PLR47_00310"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOG11402.1"
                     /translation="MPTKSIKSTSSTYKESGVDIETANAFVERIKPLIKTTARREVLS
                     GIGGFGGLYRFDTAKARNPILVSSTDGVGTKLKIAQLTDRHNTIGIDLVAMSVNDVVV
                     QGAEPLFFLDYLATGKIETEKSVQIVEGIVQGCKQAGCALLGGETAEMPGFYPPGEYD
                     LAGFCVGVVESEKLIDGSTISINDRVIGLASSGLHSNGYSLARRVLLEKGGLSVKDSV
                     AELGRSVGEELLEPTRIYVKPLLNVFKSFQVKGLVHITGGGFYDNIPRVIPDVCRCVI
                     RKESWPIPPIFSVIQEIGRVDEREMFRVFNMGIGMMMIVPEKDVQEILDRLKGLGENA
                     FLLGTIEKRDENGPPVCFTDL"
     gene            19711..22173
                     /gene="recG"
                     /locus_tag="PLR47_00315"
     CDS             19711..22173
                     /gene="recG"
                     /locus_tag="PLR47_00315"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOG11403.1"
                     /translation="MESFKKNLQRIEQPLNFAAKDQFKNLPNIRNLGKSLSGLIALQI
                     SAIPPAAKNIFGPALDHMMRIFQEYDGKDLQQKKSGIAEASLILERLKYAADNFDARN
                     PGNLQEEQIAARVEDLKISAEKLSAPVQFLKGVGPKMAERFSAKKIQTVEDLLYFLPR
                     TYEDRREIRKIHKLETNRIQTIMAAVADCSFRCYGKRRILEVAVSDNTGVLTAKWFKG
                     RMSHLAGAFQKGTRVIFTGNVTPGYAGKSMIHPDYEILDKEDEENLLNFRRIVPVYSE
                     TEGLHQKYIRKVMALALENYSRYIASPIPADICRKRGLINIREALVGAHFPGSDALME
                     PLQNARSEAHRRLIYDEFFFFQLGMAVKKSGRVLDRGISFAAGGSRLAKFYASLPFEL
                     TGAQKRVVDEIHKDLHAPTAMNRLLQGDVGSGKTLVAMSAMVTVCENGYQAALMAPTE
                     ILARQHEATLSAWAGPVGLRVVLLTGGMNGAARTEALERIGSGEADIIIGTHALIQEN
                     VDFRKLGLVVIDEQHRFGVMQRVALRNKGLSADVLVMTATPIPRTLAMTVYGDLDVSV
                     IDEMPPGKKPVRTLVMGENRREAVYEAIRKELAGNHQAFIVYPLVEQSENLDLKDATK
                     MAEHLQKDIFPNVRVGLIHGKMKDREKDAVMRDFQENQIAILVSTTVIEVGIDVPRAS
                     LMVIEHAERFGLSQLHQLRGRVGRRDIPSTCILLADYKASGDARKRLKIMENTTDGFV
                     LAEEDLAIRGPGDFLGTRQSGLPDFRIASIIRDARILNDAKEDAFALIVRDPLLAKPE
                     HAILKETLIAKWQGKLDLARTG"
     gene            22426..22731
                     /locus_tag="PLR47_00320"
     CDS             22426..22731
                     /locus_tag="PLR47_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11404.1"
                     /translation="MKTSRMIALFIAVVFLAFSTMAFAAGPGKKPDRVPPKLQSPLLG
                     SEMLWDRPEAFGPVPKSKRNLGKQVCGADSKAIGYHPKAQDEKGKVFPKGGFLCAPK"
     gene            23019..23936
                     /locus_tag="PLR47_00325"
     CDS             23019..23936
                     /locus_tag="PLR47_00325"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOG11405.1"
                     /translation="MRIAVVGIGGVGGYFGGKLAREYAASGKHEIVFIARGEHLRAIQ
                     KNGLQLCAREGQYTAWPNIATDDPSVAGRFDLVFFCVKSYSLEESARSLAKNIGKTTV
                     VIPLLNGVDSAERLKGVLPGADVVSGSVYIISFLDQPGVVIQTQGACRLIFGTDDRVS
                     ADRYSPVLELLLGAKIDARLSGRMTEALWMKFLMMCPLGSLTAATGKTYGLIRDDGDL
                     QNRLRAMMLEVAAVARARRVHLPDSAVDKTIELIGGFSHDAKTSMQLDRERGRQTEVD
                     TLTAFLCRAGREAGIPTPVHDEVYRQLTS"
     gene            24078..>24758
                     /locus_tag="PLR47_00330"
     CDS             24078..>24758
                     /locus_tag="PLR47_00330"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOG11406.1"
                     /translation="MKNISIGLIGLGNIGTGVVRLMKQNEKLIARKLGAKLVLKKIAD
                     IDINSPRGVKIPKNLLTTDAREILNDSDIAIVIELMGGYEPARTFVLEAIRNGKHVVT
                     ANKAMLATYGNEIFPAAQKQNVDIGFEASVGGTIPIIKTLKESLVANRIQSIVGIMNG
                     TCNFILTKMTNEGKPFDVVLKEAQELGFAEADPTFDIEGIDTSHKMAIVLSLAYGKKV
                     RLKDIYLEG"
BASE COUNT         5542 a         6746 c         7022 g         5438 t
ORIGIN      
        1 tgtcaatacc tattgcggca tttccagttc atacacgacc agaacgcttc tctcgctgag
       61 cgtcgcgccg acggactgga ccagcgccag gacaatttcc ggtttgccgt cattgtccac
      121 gtccttgaag cagtagtccg cgacatagcc gttgattttc ttcgtccgcc agttttccgc
      181 catgccgagg ccgtcccact ccaggttata gatttcgctc gaggagaaaa gcttcaggtt
      241 tttaaagatg cgtccgaccg acgaaaggtt tttgacgatg atcatttcct ttttgccgtc
      301 gttgttcgtg tcgaacatca cgattctcag attggcaaaa acactgtcct cctgcgtggc
      361 ctccgaagac tgctgcttct gcttgtccac gttgtcgatg tagttgttgc tgccgccgta
      421 ctgggagtcg cttctccaga tcagttcatc ggggctgaat ccaaaggaaa aaatccggct
      481 cagcggcttg gtcgtcggcg taatgatgca caggtagtcc agctcgtcga gcgcgatgat
      541 tttctcgctg gttcccgcgc ccagcgtgtc aagggtcagc ccgtaaatag acaatccgac
      601 cgggattttc tgcttatccc cctcgacgta tttcccgtcc cgccagacga tttcataaat
      661 ctgcgtatcg aaaacccggc cgccctggcc gtcgtgaaag cttcccagtc cataggcctg
      721 ccccagcaga acgggcgcgc ccgaggaagg gatgatcacc ctcaggaaat aacggatatc
      781 ggaggcaatt gttttgtagt cgccgtcctc gtactgaaga acgaaagaat tgagcagccg
      841 gtcgttgagc gacgtcacga tgatctccgg cgtgccgtct ttgttgatgt ccgccacgtc
      901 cacggagatg tatttatggt aggattttcc cttgatctgc ttgagcagct gcaagttgtc
      961 cttggtcttc aggtaaatat atacattgtg ggggtcaatg atgactactt cgttccgacc
     1021 gtcgcggttg acgtcgccga cgtccatccc gttgaattcc gtcttgaact gctggctcat
     1081 ccagaagccc ttcttgcgat cgagcggatc ggacgaattg atgaattccg cgttgatcat
     1141 cgaagtcagg gttcctttgc cttttccgga tttcatgccg gcgatgatct gcgcttcgcg
     1201 ggacgcgccg ggaacaggcg gcggggcggc ggccggcgcg gtggacaccg cagccggagc
     1261 gctttccgcg gcggcccctc cggtgatatg ccggacgatg cgctgcgaaa aatcgttgat
     1321 tttgggaatg acttcgtcga gggtctggga ctgggagaaa atgccgacgt ccgatttccc
     1381 tccggtgatg tcgatcagct ttccgtcgat gctgatgctt tttccgatct tggtaatgct
     1441 gccccagacc acgtagtcgg aattcaactg ccggccgatg tcctgaacat ccgccaggcc
     1501 gagctccttt ccgcctgttt tcttcaggac gtccgaaacg acttctttgc gcgtaacatc
     1561 aattttgtcg gctccggaaa tccggctgat cagcatgtcg ttgattccct gacgcacata
     1621 ttcaatgttt tcagcgctgt gcagcgcgaa gggcagaatt gtcaccgtgt atttttcctt
     1681 tgccgtgagc gtcgccgcca tcagcagcgc cgctagaagg caaaaaccga tcattatttt
     1741 tttcattttt tcactccgtt gttgacggct tttgataatg gaaactgcct gtgcttctag
     1801 cacttaaaaa ttgataattc aagggtggtt ttcagcgcgg tgggcggcgg cggggacggg
     1861 aaaaagaatt tagaaataaa aaatcgtgtt gtcagatcag cgcctttcat gtaaagaaac
     1921 atttacaaaa cggctggatg ggccggggtt tgcaaggggt gaaggacaaa atggaaaaag
     1981 caacgctgaa gctcaatgga atgacgtgcg agctgccgat cgttgaggga acggaaaacg
     2041 aaaaaggcat cgacatatcc agactgagac aggatacggg tctgattaca ctggacgtcg
     2101 gctatcagaa caccggctcc tgcatttcct ccattacgta tatcgacgga gacaagggaa
     2161 ttttgcgata ccgcggaatt cccatcgagg agctcgccga aaaagcaagc tttaccgagg
     2221 tggcctacct gctcatctac ggggagcttc ccaaccggga gcagcagcag cttttttccc
     2281 agcggctgac gcagaactcc atgattcacg aggaaatgct gcggttcttc gacgggtttc
     2341 cgccgaccgc ccatccgatg gcgattctgg ccacgatggt aaattccatg tccgtgtact
     2401 acacggatta tttcacggaa gatttccggg ccgagacgtt tgatctgatg gccgcgtcgc
     2461 tgatttcccg catccgcacc atcgcggcgt actcctacaa gcacagcatc ggccacccgc
     2521 atgtctatcc caaacgggac ctgaaatacg cgtccaattt tctctacatg atgttcgatt
     2581 ctccggtgaa tccctatgtg ccggatccgg acatcgagcg ggctctttcc acgctgatga
     2641 ttttgcacgc cgaccatgag cagaactgca gcacgtccgc cgtccgtctg gtgggcagca
     2701 gcatggccaa tctgtacgcg tcgatctgct ccggcatttg cgcgctctgg ggtccgttgc
     2761 acggaggcgc caaccagaag gtgatcgaga tgctggaaga gatcaaaggc agcaagctgt
     2821 cgattcaaag ctgcatcgat tcggccaaag acaaaacgag caagttccgg cttttcggct
     2881 tcggccaccg ggtgtataaa aactacgatc cccgcgcccg gattctgaag cagtactgcg
     2941 aaaccatttt caagaagcac aattatcagg acccgctgat caatatcgcc atggagctgg
     3001 aagaggcggc cctgaaggat nnnnnnnnnn caatgtcgat ttttacagcg gcatcctgta
     3061 ccggatgatc ggcattccca ccaatatgtt cacggtcatg tttgccatcg gccgtttgcc
     3121 gggcatgatc gcgcagtgga aagaaatgca tgatacggtg cccttgaaga tcggacgtcc
     3181 gcggcagatt tataccggca gcaacctgcg gcactacatt ccgatggatg agcggggata
     3241 gcgggggatc cgataaaaaa accggtggaa aatatttttc ttgacttttg cccgctgttt
     3301 gcctatatgc cgcgcaaggg aatgatgcaa atgatgaaaa cacagttcaa cgttcagaca
     3361 gtcggctttt tcttctttag ctttatcggc gacaggatct gcctggacgt atcatgacct
     3421 gacatcaagg gaaatgaacc gtgggcggat gaaaatttgc ccacggtttt tttttattta
     3481 aaaatcgcaa aaccaaaaca gggggagggg accatgaaga caatcggcag gattttactt
     3541 gtgatgggca tcttggcgat gacggccttg acggggcagg ccgtcgccca gaccaaagcc
     3601 atcaaagtcg gggcggccat caatctcacg gggccggcat ccagttgggg gcagtttcat
     3661 gaaaaagggc agcgggacta cttccgctac gtcaatgagg aaaaaggcgg cgtgggcggc
     3721 cgcaaaattg aaatgatcac gatggacacc gcctacaagg tgccggaggc gcaggccgcc
     3781 gttcaaaaat tcattctgca ggacaaagtg gacatgatcg ccacgtgggg cgccggcgaa
     3841 ggtctggccg cgaaacccat cgtccagaag cacaaagtgc cgacgatcaa ttactccacc
     3901 agttgggaaa tcctggaaaa gccggtgggc tacatgtatc tgccgttcgg aagttacaag
     3961 atggattgcc gggcggtcct tgaatacatc aaaaccattc acacgggcaa tggggcgccg
     4021 aaagtcggtt tgttgaccta caacaatgcc tacggacgat cgattcacgc acccagcagg
     4081 gaatacgcgg caaagctggg cgttcaactg gtggcgatcg aggaattccc gcccagaacc
     4141 gtggacctca acacggagct tctccgcctg cagaaaaaag gcgcccagta cattttcatt
     4201 cagatgctgc cctccaccat catcaccgcc ttcaaaagcg cggaccgcat caaatacaat
     4261 ccgctgtttc tgggaacctg gacatccacc gatccggact tcttcaggat gggcagaggg
     4321 ctgattcgcg accgcctggt catgcagttc cccggcggat tgccctcgga caaagccaag
     4381 gggatggagg tgatgaagga attgtggaag cgctacaaga cggtcaaaag ttttgatgcg
     4441 tcctactggg agggcgttgt ggtcggcatg atcatggaaa gggcgtttct gcgggcctgg
     4501 gaaaaatcaa aggtgatcaa tcccgagacc gtcaacgccg cgatggaatc catgaaaaat
     4561 gaagatttcg gcggactggt tccggcggtc agctactcga aaacgaacca tgagggctcc
     4621 ttcaccgccc ggatggtcaa agtgatggaa aacggaacgt atattccctt gacaaatttc
     4681 tacattccgg gaaaagacaa gatcaaagtt ctgaagcgat agggcaaaag gcgcgcgggg
     4741 accggatcat gcggtcttcc cgcgccaatg aagcgcggcg ggccagcccg tccagaggcg
     4801 tttatgaaag cattccgcag ccggccttcg atcatgatca accggcccca ggaagataaa
     4861 aaagccgaac tggagattcg caatctgcgg ctgagtttcg gcggcgtggt ggcggtcaaa
     4921 gacgtggatc tgtccgtcca caccggcgaa ctcacggcgg tgatcggtcc caacggcgcg
     4981 gggaagacca gcctgctgaa ctgcattacc ggcttttacc gccctcagca gggcagcatc
     5041 gttttcaacg gccgggatat tacgcacctg cacacgcacc agctcgtcgg catcggcatc
     5101 ggccgaacgt ttcagaacat cgagcttttc ccgggcatga cggtgctcgc caacctgacg
     5161 ctggcccgcc acatccactg ccgctacagc tttctgcatt cgtgcctctt tacgccgtcc
     5221 gtccgcgaag aagaaatccg ccatcgccgg attctcgagg aaatcatccg caagcaaacc
     5281 gtcggctctc tgccgtacgg aatgatgaag cgcgtggaat tggggagggc gctggcgctg
     5341 gagccgcggc ttctgattct ggatgaaccg ttcgcgggaa tgaatctgga agaaaaagag
     5401 gacatggtgc gctttctcct ggagctgaac ggacgatggg gcctcacgat ggtgctggtg
     5461 gagcatgaca tgtccatcgt catgagcatc tcccggcacg tcatggtgct gaactttggc
     5521 gagaagctgg gggagggtgc tcccgcggac atcagcgcca atccggaagt gattaaagcg
     5581 tatctcggcg atgcgcagtc gctggaataa agaggacgaa gcgctttgga caaggcaacc
     5641 ccgcacccac ccaaaccaaa ccagatcacg ctgccgcagc ttctggccgc aaacgccggg
     5701 aaatacggcg acaccagaac ggccatccgc gaaaaagcct tcggcatctg gaagaaatac
     5761 acctggggtg aatatttctc cttcgtcaaa agaacggcgg cgggcttcgc ctctctgaac
     5821 ctggcgcggg gagacaatct gtgcatgatc gtcaacaatt cgccggagtg gctcttttcc
     5881 gagctggccg cgcatgcgct gggcgcgacg tcgctcaatc tcttcacctc ttccatcgcc
     5941 gaggaactgg ccttctccat cgggcgcgtc cgctcccccc tggtcgtcgt gcaggaccag
     6001 gagcaggtgg acaagcttct ggaagtcaaa gacaagctga cgcaggtgcg attcgtcgtg
     6061 tacgttgatc ccaccggcat gacgtcgtat gaagacaacc cctggctgat cagcttcgcg
     6121 gcgcttctgg aacggggtga aaggcacatg gccgatcaac cggattatgt cgatgcggaa
     6181 atcggcaaag ggaggccccg ggacatcgcc gtcatgatgc agacctccgg caccaccggc
     6241 gtgccgaaaa tcgcgatgct ctcgcaccac aacctggccg ccatggcgca tcagtgggtc
     6301 gaagccgcgc acattcggcc ggaagaaaac tggatctcca tgtcgccgac ggcctggatc
     6361 gtcgaccaga tgtggggcat gggcgtggcg ctcgcgggcg cgatgacgat gaactttccg
     6421 gaaacgccgg agacggttct ggaggatttc cgggatatcg gtccgtccct gctcattacg
     6481 gcctcccgct tctgggagga catggcttcc cgcatccgcg tgaaaatcac cgacgcgggc
     6541 tggctcaaaa ggaagctctt ttacctgggg gaaagcgtcg gcagagaggt cgtcggaaaa
     6601 aaattgcgca gagaaagcat ttcctggccg ctgggcctct tgtaccgatt cctgacgctc
     6661 gcggtttaca atcctttgct ggaccgtctg ggctgttccc gttttcacag cgcctttacc
     6721 ggcggccacc cgatcagccc cgacgtcatc gggtttttcc gggcgatcgg cctgaatctg
     6781 aagcagtgtt acgggctgac ggaatcctgc gggatttttc agattcagcc ggacgacgag
     6841 gtcaagctgg aaaccgtcgg caagccgctt ccgaacacaa gcatcaaact ggccgaggat
     6901 caggaagtgc tggtcaagtc ggaggccaat ttttccggat actacaacga ctaccagtcc
     6961 acggaggggg ctacatcgac gacgacggcc acctgctgat tatcggccgc aaggaagaca
     7021 tcatccgcaa caagagcggc gacgccttct cgccggactt catcgaaaca aggctcaagt
     7081 tcagccccta tatcaaggaa gcggtcacgt tcggcgaggc gaggcccttc atcaccgcca
     7141 tgatcaacat tgatctgggc aatgtcggca gctgggcgga agaaaggatg ataccctaca
     7201 cgacctacat ggatctctcc cagcagaaag ccgtggagga aatgatttta cgggaagtcc
     7261 gcgaggtgaa cggccagctt cccgaggtga tgaagatcaa aaagttcgtc ctgctctaca
     7321 aactgctgga cgcggacgac gacgaactga cgcgcaccgg aaaagtgcgc aggcgcttcg
     7381 tttacggcct gtacatcaag atcattgaag cgatgtatgc aggagcgcaa aacgtgccgg
     7441 tgacggggaa aatccgctac cgcgacggca gaatcggcga gatcgaaacc atcatcaaca
     7501 tcatcagcgt ggattgagga accggtttat ggaatttttt ctgcaattgc tggtcaacgg
     7561 ggtctgcatc ggactgctct acggcatttc cgccatgggc ttcgtgatga ttttcaaagc
     7621 gtccagcgtg ctcaactttg cccacggcga actgctggcg ctgggggcct tctttttcct
     7681 gtccctggtc acctggggaa aaattccggt cgcggcggcg tttcttttga cgctgatcgg
     7741 ttgttttgtc ctgggcgtcc tgattgaaaa attctttttg cgtccgctca tcggcgaaaa
     7801 actgattttc gtcattatgc tgacggtagg gctggccgcc atgttcaagg gcctgattct
     7861 gcttgtctgg ggcggcaacc tgcacaccta tccggagttc cttcccgcgt cgctggagtt
     7921 tcatctgggc tcaatctaca tcgcgcccgt gtattccgcc acgctggtca tcagcgtcgt
     7981 atttctggtt ctcttcggcc tgttttttaa aaagtcgtca cagggcatct tcatgcgttc
     8041 ggtggcggac aatcagaaag ctgccctttc gctgggcgtt cacgtccgcc gcgtcttcgc
     8101 gctttcctgg gcgatcgccg cgatggtggc ctgcatgagc ggcatcgttc tgggcatcat
     8161 caacggcgta aacgtccacg acttgtccgc catcggcctg aaagtgtttc ccgtggttat
     8221 tctgggcggt ctggacagca tcggcggggc gatcatcggc ggaatcatca tcggccttct
     8281 ggaaaccttc acgggcggct atctgtcccc gtcgctgaga gacgtggttc cctatatcgt
     8341 gctggtgttc atcctgatgg tgaagcccta cggcctgttc ggcctggtgg agattgaaag
     8401 agtgtaatat gaagaagccg tcttttcatc cctgcgggaa tttccgcgaa acctacgccg
     8461 aggagctggc gattttcgac acctggttcg gccgggtcag cctcattgtc ttcctgcttc
     8521 tgctgttttg cctcgcgccg ttgatgctgg accgttatct gctgtacatt ctgaacagca
     8581 tcgggatcat ggcgattgcc gccatcggcc tcaacattct catcggctac accggacaga
     8641 tctcgctggg gcatggcgcg tttttcggcg tgggcgccta cgcgggagca attctggcca
     8701 cgcgcctgaa tctgccgttc ttcgtctgca ttcccgccgc cgggctgatc accgccatgg
     8761 tcggcatgat tttcggaatt ccttcgggcc gcctcaaggg gctgtacctg acgatcgcca
     8821 cgctggccgg ccagttcatc atcgaatatg tcctgatcca gtgggaaagc ctgaccaagg
     8881 ggacgatggg gatcacgctg ccggagccgc aggttctggg cgtcaacatt tccggagaca
     8941 aggccttttt ctttctgatt ttcgcctgcc tggtgtgcat gacctggttt gcggtcaaca
     9001 tcatgcgcag caaatacggg cgcgcgtttg tcgccattcg ggacaacgac cgcgcggcgg
     9061 agggcatggg cattcctatt ttccgctaca agcttctgtc gtttgcgatc agttcgtttt
     9121 acgcgggctt cgccggctcc ctctgggcct actacatggt cagcatcacg acggagccct
     9181 tcaatctcgc gctgtccgtg gaattcatcg ccatggtcat catcggcggc atgggcaaca
     9241 ttcccggcgc gattttcggc gcaaccttca tcacggtgct caacgaactg ctgcgcttcg
     9301 cgacgggcgc cctgatgaac gtcggctgga ttgccggact ggggctgaac atggcgccgc
     9361 ttcgagaatt tgttttcgga ctggcgattg tgctgtttat tttactggag ccgaaggggc
     9421 tggcggagct gtggcgcatc gtgcgctcca gcttccggct ctggccgttt tcgtattagt
     9481 aagttagaaa ttattttctg agttttttgc agaaaataat ttcgttaacg cacttatccc
     9541 gcataacggg gtcaggttga tggtatgggc gaaattcttc tgcagttgaa caatatttcc
     9601 gtcgtttatt ccgacgtgat tcaggtgctc aaaggcgtgt cgctttcggt tgctaacggg
     9661 catatcgtat cgcttctcgg cagcaacggc gcgggtaaga ccacaaccct caaggcgatt
     9721 tccggccttt tgaagcctga aaacggggag gttaccgacg gcagcatcat cttcaacggt
     9781 gtgaatattc agaatcaggc gccggagttt attacccggc agggcatcat tcaggtgctg
     9841 gaaggccgcc agccttttaa atatctgacg attgaagaaa atctgcgcgt cggaacggcc
     9901 acccggccgg gcaagcccta caaggaagac ctggaaatgg tttattccta ctttccggcg
     9961 ctggtgaaaa gaaagaaggc gctggccggc tactgcagcg gcggcgaact gcagatgctg
    10021 gtcatcggcc gggcgctgat ggcgcatccg cagcttttga tgctggacga gccttcgctg
    10081 gggctcgcgc cgctggtagt gcaggaaatt ttccgcatca ttcgaaagat caatgaagag
    10141 cacggcacaa cgattgtgct ggtggagcag aacgcccata tggcgctgca ggtagcgcat
    10201 tacggctacg tcatggaaaa cggcaaaatc gtcatggagg gcacggcccg agagcttgcc
    10261 gacaacccgg acgtcaagga attttacctg ggaatggccg cttccggcat cgccaaatcc
    10321 tacaaagacg tcaaatccta ccgccgccgc aaacgctggc tgtaaaagga gaagggacta
    10381 tgaagcggtt atacgatgaa aaagaagcgt ggagtccgga aaagagaaaa gcctggcagg
    10441 atcggagatt gtcgcgcatg gtgaggctgg gctatcagaa gtcacctgcg cttcgccgaa
    10501 tgctggacgc gaacaaaatc aatcccgccc ggataaaaac ccgggaagac ctggaagcgc
    10561 tgcccgtcac ctcgcgggaa aagctggtgg agatggaaat gcgcaagccg ccctacgcgg
    10621 gcctggaaaa ttcaaccatc gccgtcgacc gtattttcac ttctcccggg ccggtttacg
    10681 aaccccattt atcggaaaac gattttctct gggcgcgcgc tttctttgcg gccggcatcg
    10741 gttccaggga catcgcgctc aatgcgtttt cctatcatct ggtggcggcg ggcctcacgt
    10801 ttcacaacgg cctgcggaaa gtcggcgcca cggtggttcc ttccggcacg tcctcctcgc
    10861 agattcaggt tcagctcatc cgcgacctga aagtgaccgc ctacgtgggc acgccaagct
    10921 tccttatgtc cattatcgat cagtccaaag agatgggcta tgatttcaaa aaggattttc
    10981 gcgtaaagaa ggcctgcttc gccgccgagc cgctcctgcc ttcgctgcgg cagagatttg
    11041 aaaaggaata cggaatcaga acctttcaga tgtacggcgc caccgaagtg ggcgacgtgg
    11101 cctacgaatg ccccgccaaa tccggctggc atatctgtga ggaaacgatt gtggaaatcg
    11161 tcgatccggc aacgggaaag aatgtcgcgc cgggcgaact gggcgaggtg gtcgtaacgc
    11221 gcctcaatga tcttttcttt ctgctgcgct tcggaacggg cgatttatcc cggctcgtca
    11281 ccaaaaaatg ctcctgcgga agaacctctt tccgactggc gggaatcgcg ggcagggtag
    11341 gcgatgccgt aaaggtccgg gggctgttca ttgctcccag ccagatgaag ctcctttccg
    11401 ccaaattcga aggccttccc ctgcaggccg tggtcagccg caaaggccat gaagacgttt
    11461 tgacgctgcg catcgagaaa ataaattctc aagtaaatac cgacagctgg aaaaataact
    11521 tcaaaaaaat atttaaggaa ttgtgctcgg tccgaatcga cgttctggag gttgtggaaa
    11581 aaggcggaat caaaacggat cagaaggtga tcgtcgattc ccggacgtgg tagggggctc
    11641 tgtctttttg ccgaggcgat acgcgtcaat cgtcgaggag atccacaaaa acaaaatcaa
    11701 aatcagcaga cccagggcaa ggttcaaatc gattgtccgg agtgcattct ccgcgtttcg
    11761 gagaatgcgg gtcgtgcctg cgccttccga tgccgcatcc cggacgatca tgagaacaac
    11821 ctgaaaaatc atccaggcca gggtaagcgt tgcggccgcc gccggaatca gaaaagccat
    11881 tcctctcgca tatcgcttca gatagatctg gcctccgccc ggaatgagga gaaagctgaa
    11941 cagggcggcc ctggtcgcat cattcatggc gactcctcag gcgacgatgg ccgcgtccct
    12001 gattttcaat tgaacgtcgg atgagccgtt ccagtagttg atctgaggag agaagaccac
    12061 gtcgaagctg gcctggttga tcacgcctag atatttgccc atgccgaacc agatggcgct
    12121 gtgggacgcg ccgtcgtcaa ccacgcgcat tttgagatgg ttgttgccga caatgacggg
    12181 agatgaagcc ttgatgtttc tcgagcagag gaccggttcg ggattctcgc agccgaaagg
    12241 cgcgagcatt gacatctgcc ggatcagatc gaggctgatg tcctgcaggc ggcattcggc
    12301 gtcgatcagg gtctgcgaaa ccatttccga ggactgcacc gtgttgcgga tgatttcatc
    12361 cagcaggcag gcgaattcgt cgatgtcgtc ttccttgatg gaaatgccgg ccgcccggta
    12421 gtggccgccg taggacagaa gcagcggcgc gcactgctgc aggcccttgt ggatgttgaa
    12481 atcggagacg ctgcggccgg agcctttgcc cacgccgtcc ttcaggctga tgacgaaggt
    12541 cggacggctg aagagatcca ccagacgcga tgccacgatg cccacgacgc cgggatgcca
    12601 cttttcggag gaaaagacca gggcgctgat gttttccagg tcgggtttgg cgccgagctt
    12661 ttccaggatg tcgttcagga tgtctttttc catggcctgg cgccggcggt tgtgcaaatc
    12721 cagcttctcc gcgagcggcc gtgcctcctc catcgtttcc gccagcagca gttcgacggc
    12781 atcgagcggc gaggcgatcc ttcccgccgc gttgatccgg ggaatcaggc agaaggaagc
    12841 cttgaaggaa tcaacgagct gtccgtcgat gccgctgact tccttaagcg ctttgattcc
    12901 ggggcgtctg ccttccgtga tgagatccag gccgattctg gcgaagattc ggttttcatc
    12961 cagaagcggc gcgatgtcgc cgatggttcc cagagcaacg acgtcgagaa attctttcag
    13021 gttgggataa tcgtcgtttt tccaaaagcc ttccctgcgc agcgttccgc gaagcgcgat
    13081 caggaaattg aaggcgatgc ccacgccggc cagatgtttg aagggaaagg aacagtcgga
    13141 acggttggga ttgatgcagg cgacggcagg aggaagctga tcggcgattt catggtggtc
    13201 caggacgatc gtatcgatgc ccacggattt ggcgtaggca atctgctcga tatcggagat
    13261 gccgcaatcc actgtaacaa tcagcttcac attcttttgt ttgaaccggt cgatgaccgg
    13321 aatcttcagt ccatagcctt cctggacacg gtccggaatg taataggaga cgcgggcggt
    13381 gagctgtttc ataaatttgt acaaaatgac gacggaggtg attccgtccg cgtcgtagtc
    13441 gccgtaaatg acgatctctt cgccgtcgta aatcgccttc agcagcctgg ccaccccttt
    13501 tttcatatcc tgcatcagaa acgggctgtg gaggtcgttg agggaaggat gcagatagcg
    13561 gtgcgcgtct tccaagctcc cgacgcgccg tttcaggatc atctgggaaa taatcgggtg
    13621 gaagccgaat tctctgacca gcgattcttc tatcgtttta gggccggaat ccttgatttt
    13681 ccattgcgtt accggcagtt gtccccgttc attcatgtgt tcttctttaa cgttttattt
    13741 ttcatcgcaa tcatatcagt gatcgttcat atgtcaagtc ttcattggtc ttggcaggcg
    13801 ttcttgatct ttaaaacaat aaatgctaat cgttttgctg taaatttcga gacaataggg
    13861 cacccccatg ctgatgttaa cccatacctg tttattgcag caaatgatga gcgcctccgg
    13921 aattcaaaat atggatccgg atatttacat ttataatatc gccccggatt tattgacgat
    13981 tcatcccgat atcaaccccc ggcagactca ttccattccg cgtttcatcg aggcgccgcc
    14041 cgaccatctg cgaacggcct acatcatgtt tcatctgctg gttgatgatc tggcgcatta
    14101 cggcagcatc tcgatgacct gcaaggaagg tttcgatccg gactcctcgg gctataccta
    14161 tctcaagggg cggaatcttg tccgctccat catggagctg catgccatca tcggcaagga
    14221 catctcctac agcgaggccg cttaccgttc tcatttaatt attgaaatga tctatgacct
    14281 gatcattggg ccgcaaatcg acaaaaacgg ttccatcgac cttctggaag aggccattca
    14341 ttttacattt gaccggaggg aaaaggaatt ttgctctgat atttcatggc tttacggcat
    14401 cgatttcgaa caaagccggg aggtgctgaa aattgcggtg tcgtacatta ccggggaaag
    14461 gctgagaagg atcatgaata tcgaaggccg gattcgcctt tttacggaca agttcggcct
    14521 tcggaacaac aatgagatgt ttgccgaagc cattgcagct ctgtttcagg acgccctgga
    14581 ttccattgaa aacgacgact ttcttcagga aaccgcttcg accattcgaa gctgcggctg
    14641 gcttccgacg cgttagcttg cttttttcac aaaaccggag cggatgcagc gcgtgcagac
    14701 ttttacccgc ttcacggctc ccgttttttc atccacgcag cgcaatttct gcaaattcgg
    14761 acgccagatc acgcgggtct tgttgttggc gtggctgacg ttgttgccga tggccggttt
    14821 ctttccgcat acttcacaaa tccgagacat gacgagactc cttttaaaaa agtgcgtggt
    14881 tactaagcca gaatggttta ttttgcaagc atttttttct tcctgaccac cttcagcctt
    14941 cggccttcag tctattcacc tgattattcc tcaccgcctt cagccttcgg ccttcagtct
    15001 tcagtctatt tacctaattt ttcatctgtg cgggctgccc ggttgattcc agcacacgaa
    15061 accagagatt tccgtccggg tcgatctgcc tgccggatat cgccgcggaa aggggcagat
    15121 gaacatattc atccctgcgc agcgccacca gcaggccggt tttccccgcc atggcggcgt
    15181 gcacggaata ctgccccaga gagctgcagt aaaggctgtc cgaagcgttg gccgggacgg
    15241 agcggatcat atagctgggt tcaatatatt tcagattgat ttccacatgc gcttcgcgga
    15301 aatgcgtttc aatggcactt ttcaaaaaca ggccgatgtc ctgcagacgg acattgccgg
    15361 aggcatccgt ttctcttttc ttttcatcct gctgcatcaa ttcctgcccg gcgccttccg
    15421 ccaccagaat cacggcgtgt ccgcttgcct tcattcggtt ttccagcgct ttcagaaacc
    15481 ccttttctcc ctccaggtca aacggcactt cgggaatcag aacaaagttg gcgtctccct
    15541 gggccagcgc ggtcgtggcc gtgatgtagc cggacaggcg tcccatgatt ttcaccaggc
    15601 cgatgccgtt gtaggcgccc ttggcctcga catgcgcgct tccgaccgct tcgacggttt
    15661 tggcgatggc ggtgtcgaat ccgaaggatt tctcaatcag gttcaaatcg ttgtcgatgg
    15721 ttttggggat gccgataacg gcgatgggat ctttccgggc atggatctcc tccgtgattt
    15781 tttccgcggc gcgcatggtg ccgtcgccgc ccacgcaaaa aagaatgtcg attttcaaaa
    15841 gctccaggta gtccaccatc accttcacat cctgaggacc gcgggacgag gacaggatgc
    15901 tgccgccgat atgcgtgatg tccttgacga agtcgggtgt gagctggatg ggtattttgc
    15961 cgtattccgg gttcagtccg cgcagtccgt atttgattcc cagaacgttt tttacgccgt
    16021 aacggtaaaa cagcgtcatc gtcaccgagc ggatcacgtc attgatgccc gggcagaggc
    16081 cgccgcacgt gacgatggcc gctttcgtcc cggcgggatc aaaaaatatt tttcttcggg
    16141 ggcccgccgg ctccagagac aaaggctttc cggttgaaac catcagcgcg tcgcaatctt
    16201 tcaggcgggt gttgaacagg atccggtcat cgtccgaaac aaatccgttg atggcaagcg
    16261 gcgaatcgat ggcgcagacg cccagaacgg aaatggcgaa gtcgtcttgc atacgattat
    16321 ttccctttct gcaggaccct cgaaacggcg tttctgccgg aaaggatctc cgcttcaaaa
    16381 ccggcgtccg cgagcagcga cgtcccggtc aaaaagacat tgctccatct cgtccggtcg
    16441 ttcttggccg aaaaagacgt aaaaaaagcg ttgcgcaccc tgtatttcgg agagaatact
    16501 ttacggtact tcaacgaaag ttcgatggac ttgtcaaggt cgtacatctc gatgccgtct
    16561 ttcaagaaag ggaaaaactg atcgagcctt tccaggatgg attcggcttc ccgccgcagg
    16621 gcctccggag cccaggcggc atcatcggcc ggcaggtaaa ccgtggcggt catgaaattc
    16681 tttgcctgcg gcaggggttt tcctttttcg ggcatgccgg tttccagaag aatcaggttt
    16741 cggtcgtgaa gatccctatg gacgtccgcg acgacggcca tgtgacgggc catctgttcg
    16801 ggcaggaact tctgggcaac gcccagaaaa agggtaaatg gatagccggc aatgcccgcc
    16861 ttgcggaact tgtcccaaaa aaaggcgtgg ccgcgttttt gcgtgatgag gggcaatgcc
    16921 tccgatttcg tgctgacgat caaataacgg ccggcaaccc gggatgtgga tttgtctgga
    16981 gcttccatct ccagcgtgat tgttttgccc cggtcgaggg acttgacggc atgcccgctc
    17041 aggtacagac cgccctggga ttccagcgtc cggatcattt cgttgaacaa aaactgcttc
    17101 ccctgcggaa aacaggcgac gcctcgcagc ggcgcgctgt attgaaaagc ggaggcaaag
    17161 gaaaacaaat cgtccgtatt ttgcgaaagc agcgcctgct gcgcttccca tactttttcc
    17221 agcgccgcgt cccgggagag cattttgtca aacctggcgg cggcaaattt gtactgcagc
    17281 acgaagggat agattttcag ataggaaaag tattccttca gggtttgcgg ctgcacccgg
    17341 ggatgttcgg caatccagtc ctgaaaaacg gaagatgctt gaagcgcaat gccgtaaaaa
    17401 tcgcggatgt ccgtttcccg cttcggaaat tcccgggcca gttcggaaag gagcgcttcg
    17461 gagtcgtttg agaaatcgat ccggtggccc gggagcagca cctggtacgc ggggctcatg
    17521 gacgtcggat gagtttcggg aatttcaacg cccagttcgg caagcacgga aaagccgggc
    17581 tgaccgggag cgaggccggt catcggcgcg ggatcaagat ggaaaacaaa gtcgccgatg
    17641 agctgcagcc cgcccaggcc gccgggggca accaggagcg tcttgagtcc gtttcggctc
    17701 gcgcaggcgg ccgccacgtg ggaagcgaga tcatcgccca gcaccagcaa atcgtacacg
    17761 aatgacattt cctccaaaac aggccggcgg aaagcggcgt tcagccctcc gggggcggat
    17821 tgctgagcgc ggcgtcatgg tttccggcgt ccctgatctc tacgcggcga ccgtttctga
    17881 caattcggcc ctgggcgaaa taacggatgg cctgggggta aattttgtgc tcttccttca
    17941 aaatccgcgc cgcgagctcc tgggccgtgt cgccggcaaa aacgggcaca accgcctgaa
    18001 tgatgatcgg acccgtgtcc acgccttcat cgacaaaatg aactgtgcac ccggaaaaac
    18061 gaacgccgta atccagcgct ttctgctgaa cgtgcgtgcc ggggaaagag gggagcaggg
    18121 cgggatgaat attgatgatc cggtccggaa aggcacgaag aaaaaacggc gtaagaatcc
    18181 gcatgaaacc ggcgagcacg accagctcga cgccggcgcc ctgcaggatg cgaagcagtt
    18241 cgcggtcaaa cgcttcccgg ttttcaaaat cccggtggct taccaccgcg gtgggaatcc
    18301 ggtattgttg cgcacgggtc aggccgtagg cgtcggggcg gttgctggcc accatagtga
    18361 tccgggcgcc aagcgccccc ttttcgatgt gatcgatgat ggactgaagg ttggagccgc
    18421 ttccggaaat caggacgccc agttttaaaa ggtcagccat aaaaccgcct tacagatccg
    18481 taaaacagac cggtggcccg ttctcgtccc gcttttcgat cgttcccagg agaaaggcgt
    18541 tttctcccag ccccttcagg cggtcgagaa tttcctgcac atctttttcc gggacgatca
    18601 tcatcatgcc gattcccatg ttgaacacgc ggaacatttc acgctcatcg acccggccga
    18661 tttcctgaat gacggaaaag atcggcggaa tcggccagct ttccttccgg atcacgcagc
    18721 ggcagacatc gggaatgaca cgcggaatgt tgtcgtagaa accgccgccg gtaatgtgga
    18781 caagcccctt gacctgaaag cttttgaaga cattcaggag aggcttgaca tagatgcggg
    18841 tcggttcaag cagctcctcg ccgacgcttc gtccaagctc cgcaacgctg tccttcacgg
    18901 aaagtccgcc tttttccaga agcacgcgac gggccagcga ataaccgttg ctgtgcaggc
    18961 cgctggaggc cagaccgatc acgcggtcgt tgatgctgat ggtagagccg tcaataagct
    19021 tttccgattc caccaccccc acgcagaatc cggccagatc atattcaccc ggcggataga
    19081 atcccggcat ttcggccgtc tctccaccca agagcgcgca gccggcctgt ttgcagccct
    19141 ggacgatgcc ttccacgatc tgaacgcttt tttccgtttc gattttgccg gtggccaggt
    19201 aatccagaaa gaacaaaggc tccgctccct gaacgaccac gtcgtttacc gacatggcca
    19261 ccaggtcgat gccgatggtg ttgtgccggt ccgtcaattg cgcgattttc agcttggttc
    19321 cgacgccgtc ggtggaggaa accaaaatgg ggtttctggc cttggcggtg tcaaaacggt
    19381 acaaaccgcc gaagcctccg atgccggaca ggacttccct gcgggccgtc gttttgatca
    19441 ggggcttgat gcgctcgacg aaggcattgg ccgtctcgat atcgacgccg gattctttat
    19501 acgtcgaaga ggttgatttt atggatttag ttggcatagg gctccatcag gacaggttgt
    19561 attttttaca cttggcttaa gacgaaaacg atttaacgta aatccccttg cgtgtcaatc
    19621 ctcgacttgc gcaaacaaaa tttatttgtt aaaaaacaaa caaattcata aacgcatttt
    19681 ccgcaccttc cgtatccgga gcatgatgaa atggagtctt ttaaaaagaa tcttcagcgc
    19741 atcgagcagc ctctgaattt tgccgctaaa gatcaattta aaaatctccc gaacatccgg
    19801 aacctgggga aatccctctc gggcttgatc gctttgcaga tatcggccat tccgcccgcc
    19861 gcgaaaaaca ttttcggccc cgccctggac cacatgatgc gaatttttca ggaatacgac
    19921 ggcaaggacc tccagcagaa gaaaagcggc attgccgaag cctcgctcat cctggaacgg
    19981 ctcaagtacg ccgccgacaa ctttgacgcg cgcaatccgg gcaatctgca ggaagagcag
    20041 attgccgccc gcgtggaaga cctgaaaatc tccgcggaaa agctgtccgc gccggtgcag
    20101 tttctgaagg gcgtcgggcc gaaaatggct gaacggtttt ctgcaaaaaa aatccagacc
    20161 gtcgaagatt tgctttattt tctgccccga acctatgaag atcgccggga aatccggaaa
    20221 attcacaagc tggagacgaa caggatccag acgatcatgg ccgccgttgc ggactgctct
    20281 ttccgctgtt acgggaaaag aaggattctg gaagtcgccg tgagcgacaa caccggggtc
    20341 ctcacggcca agtggttcaa gggccggatg tctcatctgg ccggcgcttt tcaaaaggga
    20401 acccgcgtga ttttcaccgg caacgtgacg cccggctacg cgggcaagtc catgattcat
    20461 ccggattacg aaattctgga caaagaggac gaagaaaatc tgctcaattt caggcgcatt
    20521 gttccggtct attcggaaac ggagggcctc caccagaaat acatccggaa agtcatggcc
    20581 ttggcgctgg aaaattattc gcgctacatc gcatcgccga ttcccgcgga catctgccgg
    20641 aagagaggcc tgatcaacat ccgcgaggcg cttgtgggcg cgcatttccc cgggagcgac
    20701 gcgctcatgg aacctctgca aaacgcgcgc tccgaggcgc accgccgcct gatttacgac
    20761 gagtttttct tttttcagct gggaatggcc gtcaagaaat ccggacgcgt tctggatcgc
    20821 ggcatttcct ttgccgccgg cgggagccga ctggcaaaat tttacgcctc gctgccgttt
    20881 gagctgaccg gcgcgcaaaa gcgggtggtc gatgaaattc acaaagacct gcatgcgccg
    20941 acggccatga accgtcttct gcagggcgac gtgggaagcg ggaaaacgct ggtggccatg
    21001 tcggccatgg tgaccgtatg cgaaaacggg tatcaggccg cgctgatggc gcccacggaa
    21061 attctggccc ggcagcacga ggcgaccctg tccgcgtggg ccggtccggt cgggctgcgc
    21121 gtggtcctgc tgaccggcgg catgaacggc gcggctcgca cagaggcgct ggagcgcatc
    21181 gggtcgggcg aggccgacat catcatcgga acgcacgccc tgatccagga gaatgtggat
    21241 ttcaggaaac tggggctggt cgtcattgac gagcagcacc gcttcggcgt tatgcagcgg
    21301 gtagcgctgc gcaacaaagg cctttccgcc gacgtgctgg tcatgaccgc cacgccgatc
    21361 ccgcgaacgc tggcgatgac ggtttacgga gacctggacg tgtcggtcat cgacgaaatg
    21421 ccgccgggca aaaaaccggt gcgcacgctg gtgatggggg aaaacagaag agaagcggtg
    21481 tacgaggcca ttcgcaaaga gctggccgga aatcatcagg cgttcatcgt ctatcccctg
    21541 gtcgagcagt cggagaacct ggatttgaaa gacgcgacga aaatggccga acatctgcag
    21601 aaagatattt tcccgaatgt ccgggtcggc ctgatccacg gcaaaatgaa ggaccgtgag
    21661 aaagacgcgg tgatgcggga ttttcaggaa aatcaaatcg caatcctggt atccacaacc
    21721 gtgatcgaag tgggaatcga cgttcctcgc gcctcgctga tggtaattga gcacgcggag
    21781 cgattcggcc tgtcgcagct tcaccagctt cgcggccgcg tcggccgccg cgatattccc
    21841 tccacctgca ttcttcttgc cgactacaag gcatcgggtg atgcccgcaa gcggctgaaa
    21901 atcatggaaa acacaaccga tggatttgtc ctggccgaag aagacctggc tattcgcgga
    21961 ccgggggatt ttctgggaac caggcagtcc ggattgcctg atttccgaat tgccagcatc
    22021 atccgcgacg cgcgcattct caacgacgca aaggaggacg cttttgcgct gattgtccgc
    22081 gatccgcttc ttgccaagcc cgaacacgct attttaaaag agacgctgat cgcgaaatgg
    22141 cagggaaaac tggatctggc ccgaacggga taaacaaagg gtatgaccag agaggattga
    22201 gaaaaccggt ctgaagccat caaggttaag atttgacgag gaggaaaaca cttagaagtc
    22261 atcggtaatc tatcggtaaa aattgggcaa tcataaggat tgacgatagc cttccggccc
    22321 tgttaatttt tgcataattg aggcgtttag aaaattcgtc atgaaagaag ggggaatgac
    22381 tgtctcctca aaatgttaaa atggaaaaac agaaaggagt gcctgatgaa aacatcaaga
    22441 atgatcgcat tgttcatcgc agttgttttt ttagcgtttt caacaatggc atttgccgcc
    22501 ggaccgggca agaaaccgga ccgcgtcccg cccaagcttc agtccccgct gctgggcagt
    22561 gaaatgctgt gggaccgccc ggaagctttc ggccccgtgc caaagtcgaa gagaaacctg
    22621 ggcaagcagg tatgcggagc ggacagcaag gccatcggtt atcatccgaa agcccaggat
    22681 gagaaaggca aagtttttcc gaaaggcggt tttctctgcg ccccgaaata agcgggtttt
    22741 gaaacctgaa acgcagaaaa ctcacctgtg aggctgatgt tcaagcctca caggatcttt
    22801 ttgttttgac cggactagcc aaagcagcgg taagccaaga caatcgatcg aagatgcctt
    22861 ccatgaaccc caaaaacatt ttttagcggc atttcctgaa gattccataa ggcagttgac
    22921 cggagttcag ttgtccgtca acaaccggaa gtccaaattg atccttgatc tttttgaagg
    22981 atttcggtta actcgcaact gaaaaggaga tccattcgat gagaatcgcg gtggtcggaa
    23041 tcggcggggt agggggatat ttcggcggca aactggcccg ggaatacgca gcctccggaa
    23101 agcatgaaat cgtcttcatc gcccgcggag agcatcttcg ggccattcag aaaaacggcc
    23161 ttcagctttg cgcccgggaa ggccaatata ccgcctggcc gaacatcgcc acggacgatc
    23221 cctccgtggc cggccggttc gatctggtct ttttctgcgt caaaagctat tcgcttgagg
    23281 aatcggcacg ctccctggcg aagaacatcg gcaaaacaac cgtggtcatc cccctgctaa
    23341 acggcgtgga cagcgccgaa aggctgaaag gggtgctgcc gggcgcggac gtcgtcagcg
    23401 gttccgttta catcatcagt tttctggacc agcccggggt ggtgatccag acgcagggcg
    23461 cctgccgatt gattttcggc acggatgacc gggtgtccgc cgaccggtat tcccctgttc
    23521 tggaactcct gctgggggcg aaaatcgacg ccaggctatc gggccgcatg accgaagcgc
    23581 tgtggatgaa attcctgatg atgtgccccc tgggatcgct cacggcggca accggcaaaa
    23641 cgtacggttt gatccgcgat gacggggatt tgcaaaaccg gctgcgggcg atgatgctgg
    23701 aagtcgccgc ggtcgcccgt gcccgccgcg tgcacctgcc ggacagcgcg gtggacaaaa
    23761 ccatagagct gatcggcggc ttctcccatg acgcgaaaac ctccatgcag ctcgaccggg
    23821 agagaggccg gcagacggaa gtggacaccc tgacggcgtt tctttgccgg gcgggacgtg
    23881 aagcggggat tcccacgccg gttcacgacg aggtctatcg tcagctgaca tcgtagcgga
    23941 cccggccccg gcggaggttc atctcctcat atccatttga caaaacaacg ggatttgata
    24001 tagggtacca ccctcgcgaa atcaggcggg gcaaagcggc cccgggccgt atttttgatg
    24061 cacggttgag gtaaaaaatg aaaaacatat ccatcggctt gatcggcctg ggcaatatcg
    24121 gaaccggcgt ggtccggctc atgaagcaaa acgaaaagct catcgcccgg aagctaggcg
    24181 cgaagcttgt tttaaagaag atcgccgaca tcgacatcaa ttccccgcgc ggcgtgaaaa
    24241 ttccgaaaaa cctgctgacg accgacgccc gggaaattct gaacgattcg gacatcgcca
    24301 tcgtgatcga actgatgggc gggtatgagc cggcgcgcac cttcgttttg gaagccattc
    24361 ggaacggcaa acatgtggtg accgccaaca aggccatgct ggccacctac ggcaacgaga
    24421 ttttcccggc ggcgcagaag caaaacgtgg atatcggatt tgaagccagt gtcggcggga
    24481 ccattcccat catcaaaacg ctgaaggaat cgctggtcgc caaccggatc caatccatcg
    24541 tcggcatcat gaacggaacc tgcaatttca ttctcaccaa aatgacgaac gaggggaagc
    24601 ccttcgacgt ggtcctgaag gaagcccagg agctgggttt tgccgaggcg gacccgacgt
    24661 tcgacattga gggcatcgat acgtcgcaca agatggccat tgtgctgtcg ctcgcctacg
    24721 ggaaaaaagt ccggctcaag gatatttacc tggaaggc
//
LOCUS       DAOSIM010000004        24278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_2305_length_24278_cov_7.389547, whole genome shotgun sequence.
ACCESSION   DAOSIM010000004 DAOSIM010000000
VERSION     DAOSIM010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 24278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24278
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2305_length_24278_cov_7.389547"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PLR47_00335"
     CDS             <1..883
                     /locus_tag="PLR47_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOG11407.1"
                     /translation="AELEELPKGAYNIRKNGKLLDRHVSANINIETNEDETGIIVKIK
                     PGTKNESVHIPVILSQAGLYDVVQNAFVIGDDAEVTIIAGCGIHCGSSEPEGHAGIHE
                     FKVGKNAKVTYVEKHYASGEGTGRRSLNPTTKVFLAEGARAEMELTQIGGVDDTHRVN
                     EAVLGPNSLLLITERVLTDGEQTAVSTNDIVMKGRGSRANMISRSVMKGNSKQTFYAT
                     IDAQNQCYGHIECDAIIMDNGTNETMPSLKAGHPDAELTHEASIGKIANDQLTKLMSL
                     GLTYDQAVNRIIQGFLK"
     gene            970..1488
                     /locus_tag="PLR47_00340"
     CDS             970..1488
                     /locus_tag="PLR47_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOG11408.1"
                     /translation="MDKRMNALAFALENEEKEREFYLAHARKTRNMAGKNMFKQIADE
                     EKEHYEMLKKLHAKWEEKKKWPATIALKVRPSAAGAILQGMSGKKSARIAGTDDELKA
                     VRTAIDFEARGVALYTKLAKQSTDAKEIAFFNMLASVERQHLLSLKDTEEFLADPATW
                     YQHMERSGLDGA"
     gene            complement(1590..3548)
                     /locus_tag="PLR47_00345"
     CDS             complement(1590..3548)
                     /locus_tag="PLR47_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOG11409.1"
                     /translation="MIYFRNASLSFLDKTLFDRIQWTIHPKSRIGLVGDNGAGKTTLF
                     RAILGEVPLDSGLIEIPGRKQKKIGYLPQDLVELESTGLIEYLRRKSGIAGLEEDLRS
                     LERQMAQAPSHSREYGELSSRYAQATAEFSAREGYAFEARAKQILKGFGFRPQDFEKT
                     CDVFSGGWKMRILLTSILLGRPDIMLLDEPTNHLDTESMEWLESYLKDYPGTILIVSH
                     DHFFLDKIATQIAELANRRIHIYKGNYTYYLAEKDRRFEALKKEMELQKAQIKKIESF
                     VERFRYKATKASQVQSRVKMLEKFKDLQAEGAAKSVVMKFPEGKKSAKEVVTVCDLGH
                     AYGDLAVFSGLNFSLFRGDKVAFVGVNGSGKSTLSRLLSLFERPVRGSVTYGEGVNMA
                     FFSQESAQNLNYANTIWEEVQGVPTSADDQNKRNLLGAFLFSGDDIYKSVGVLSGGEK
                     SRLALLKIMLTDTNFLVLDEPTNHLDIKTKDVFQSALIHYSGTMAIVSHDRYFLDRLV
                     NRVFELKDGSVTDYAGNYSYFIEKRARMAADAGQQERRDRAPVDRTLKTKDKKRLEAE
                     ERNRLSKIKNGLKRELAKLEEKIAELESRKAGDEHSLCDPQTHKDAVKIKGLQIDLKA
                     VENELAQAYHLWTELSCKLEETDSDGFA"
     gene            complement(3603..6350)
                     /locus_tag="PLR47_00350"
     CDS             complement(3603..6350)
                     /locus_tag="PLR47_00350"
                     /inference="COORDINATES: protein motif:HMM:NF017459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 family lipoprotein"
                     /protein_id="HOG11410.1"
                     /translation="MRKIFKVVFWGLIPAAALMLQGCLVSTPSTLITPEIRSLYEGEY
                     TVDPYMKKHVPLSVAVLPFVDLSGSKEGANAMRKGFYNHFSSLPFKDMELYKVDDLLL
                     KAGLTDPDVIRKIPPRKLGEILGVDAVVFGEVSDFDKYFAVLYSQVAVGAEVKMYDAK
                     TGNFLWSGKHKVRKHEGGVSTNPVGIIATVIATALNVRDIQLLRANDDLFRDMVKTIP
                     TPSLAQAKRPPVITLLTQDTKGLPKKAGDEIKVVIQGTPNMQASFDIGQYRKRIEMQE
                     VEPGGYYGVYKVVPGDNIEKAIVTGFLTDDSGNTAHWVDAIGSVTLDTTAPDKPAGIT
                     STGRNQRVALRWEKNTAADLAGYLIYRSETPLSGYLQIGKTELNEYKDTDQNLANART
                     YYYRISAVDFAGNESRTEQAEGMPIAPGPTPVSGAIEADATWYSGASPYILESNVTVK
                     DKALLTIEPGTEIQSKGGGLVIEGRISARGDNDRIIRFDSAQDGQFWPGITFASVKDR
                     ENHLAFVRVQNARTAVFCQASSPTIEQSEFTKNHEAIKIQGVFSKPLVSRNTLYKNKG
                     AAISVSDGAAPRIVGNTINDNDHTGVVLHSAAAEITQNTITRNKNNGIAVKGAEAVIF
                     QNNLVDNQPYNVFGEMKGEPAKALNNWWGSAAMAAVLSGIHGRVDVSSVLDAPYPQGK
                     ALAVPILDQKLEGAIQADAYLILSRSPYRLVKDVVIDGGATLYIEPGVVIEYEQETSF
                     ITRNGGIVAKGTGEKPITFTAASASPAPGFYVSAVRFSEATKINSAFAYCIVKYATTA
                     FDIYTGSPEISHSYIAHASQNGVYCRKDAAPVLSHNTFAHNQGEGAVKCVGSANPKIF
                     QNNFIDNAVAIQSFSSIFIDARNNWWGASPPDPKIFWGENINTNPWLEQANPEAFTER
                     R"
     gene            complement(6466..>7168)
                     /gene="rfbD"
                     /locus_tag="PLR47_00355"
     CDS             complement(6466..>7168)
                     /gene="rfbD"
                     /locus_tag="PLR47_00355"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOG11411.1"
                     /translation="GYTNVDACETAKEECFAVNAEAVKNIAQACRDGNVPMIHYSTDY
                     VFDGNANHPYAEEDPCNPINTYGASKLAGEHHLKRLTDNFILIRTAWLYGAKGKNFVS
                     AILDRARETGKLTVVDDQMGSPTCTRDLAAATELLVDKNARGIFHVTNRGCCTWYDFA
                     RKILKEARMEQVELSPMKTSELQRAARRPAYSVLGMHKFVAATGKTMQPWQLAFSDYY
                     SFIASRTCLSPPGTA"
     assembly_gap    7169..7178
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7255..7977)
                     /gene="truA"
                     /locus_tag="PLR47_00360"
     CDS             complement(7255..7977)
                     /gene="truA"
                     /locus_tag="PLR47_00360"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOG11412.1"
                     /translation="MILEYDGAAYCGWQRQQNGLSIQQVVEEALARIIGENATVIASG
                     RTDAGVHAMNQAASFRSRTVLPANKIFLGVNSVLPDNIVVKDLQEAPPDFNALKDARG
                     KVYVYRIQNQRLRPALGRQYFWHVRCPLNLESMRLAAMKLIGEHDFSCFCATGCDIKN
                     RVRLLTVCSIVHQESGRLEITLESSGFLRHMVRNIVGTLVDVGRGKIAPESMPQILAS
                     RDRKQAGTAAPACGLFLKEVKY"
     gene            8171..9742
                     /locus_tag="PLR47_00365"
     CDS             8171..9742
                     /locus_tag="PLR47_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOG11413.1"
                     /translation="MGDDIRQATTEGKIRDFETRAQSMMKMGGEKQVQKQHDGGKLTA
                     RERLNRLFDEGSFQEVQLFVKHHATLFGMDKKEIPADGVITGFGKVNGRIVFAAAQDF
                     TSAGGTLGEMHAKKIWKVMDMALDAQKPFVSINDSGGARIQEGVPSLEGYGGIFYRNT
                     LASGYIPQITAIMGPTAGGAVYSPALTDWIFMVKNTSYMYITGPDVIKAVIGEEVSQE
                     DLGGAVAHASKSGVCHVVTENDEDCIQKIKGLLSYLPDSCHSPLPVAETTDNPDRECP
                     ELNKIIPDRAARAYNMKSIIKSVADNGEWFELHAQWAPNIIVALIRIMGRPVGVIANN
                     PMSYAGVLNVNASDKASRFIRFCDAFNIPLVTFSDVPGYMPGTDQEWAGIIRHGAKLL
                     HAYSEATVPKITVVTRKAYGGSYIGMCCKQLGADYVMAWPTAEIAVMGASGACNIIHR
                     AEIKGAVDPDAKRAALIADYEEKFNNPYYAASLGAIEEVILPSRTRKRIVVLLEALQN
                     KKEARRTKKHNNIPL"
     gene            10089..>11431
                     /locus_tag="PLR47_00370"
     CDS             10089..>11431
                     /locus_tag="PLR47_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11414.1"
                     /translation="MKRFWLVLLSLGLIVAFSSTAMAVDVKFSGEFYAGGMYLDKTNF
                     TKSTTGGNPSTAFYYQRLRARTDFTITPGLKLITRFDAMERAWGAARSNPVGPLAADS
                     AGTVAENENIAIDWAYIDYKSPIGTFLVGYMNYGTTGTVFGNNRNTQARIRFNRMFGP
                     VNVWASISKVAENSLTAVKPASTTSDADNDVYHLEGIYNWKSGRAGLNVNYYNYANTR
                     PAPTSYKRTYFLFTPYVFAQIGPVSLQAEFNYLNGTDKYEDGVTTTVDNLDKVTLENI
                     SAYIDATANFGMFYAGGSFAYVSGNDPGTNDKNEGGLINGGYDWNPCLIMFNYDDRAK
                     WAGNLTGYGTSADNGRMANAWFFQLRGGVKPIDKLDVMASVSYANADKKPANYLYNDY
                     GWEVDLTANYKITNNLSYMVGAGYLFTGKYYKANVAVNNELNDNFLVINKLTLTF"
     assembly_gap    11432..11531
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11532..11778
                     /locus_tag="PLR47_00375"
     CDS             <11532..11778
                     /locus_tag="PLR47_00375"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOG11415.1"
                     /translation="MNFKIVLLIVVACLAVVFVIQNVSAVTVSIFFWEISLSLALLIF
                     FVLAFGFAFGWFLHSFLAYQKIKKEVKDIQNDMRLQK"
     gene            complement(11827..13056)
                     /locus_tag="PLR47_00380"
     CDS             complement(11827..13056)
                     /locus_tag="PLR47_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOG11416.1"
                     /translation="MKNYLPPFLKSYGFSILLIASIILGAIFGMIYQKEAVRLKPLGD
                     IFLNLLFTVIVPLVFFSISSTVASMTNLKRLGKILSVMILIFVITGIMASAVMIAAVI
                     FYPPASGVNIPLPATADLQQIKAGDQMVRAFTVPDFPHILSKNHMLPLIIFSMLVGLA
                     ASAAGEKGKDFSRFLASANTVMMKLISFIMYYAPIGLCAYFAYLVGVFGPELLGSYAR
                     AMAVYYPTAIGYFFLAFTLYAFIAGRAYGVKKFWTHIIPPAVTALATGSSMATIPANL
                     QAADKIGVPKDISEVVIPIGATMHMEGSCLAAVLKIAFLFGIFQMPFAGIETILTALG
                     IALLTGVVVSGIPGGGTIGELLILSLYGFPPEAFPLITMIGTLVDAPATMVNATGDNV
                     SSMMVARVLGGKEWMKG"
     gene            complement(13060..13827)
                     /locus_tag="PLR47_00385"
     CDS             complement(13060..13827)
                     /locus_tag="PLR47_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOG11417.1"
                     /translation="MKSKFFLIGLLAAIWFSAAAPPASLCEDAMPDTFVHIEEIIPDA
                     LLDIRYFTEHNFLGVKVDGYLAPACILTRPAAEALARVQKDLAPFGMTIKIYDCYRPQ
                     RAVDHFVRWAKDVGDTRTKKEFYPTVDKQNLFRDGYIAERSGHSRGSTVDLTLVPLPA
                     PIQPAYKAGDPLKECCLPAGVRFADNSLDMGTGFDCFGEMSHPENKDIGPQQRANRLL
                     LKTLMARHGFRNLPEEWWHFTLDNEPYPDTYFNFPVK"
     gene            complement(13841..14536)
                     /locus_tag="PLR47_00390"
     CDS             complement(13841..14536)
                     /locus_tag="PLR47_00390"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOG11418.1"
                     /translation="MTGIGCLLALAALWSLAACASRTAVTDRLAPYVQTAAMENVGQV
                     LLVTDESFLFLTSHKIYPLEKTDSSWRQAKNPLDAVIGRNGFAPPGEKREGDGRTPSG
                     LFRMKTAFGYPPSVPTRMPYRQSQENDLWIDDPNDPDYNRWVHQNQTKAASYERMKRD
                     DDLYKYGIVIEYNTDPVIKGFGSAIFLHVWAGTDSTTAGCVAASEDDILKMLGWLNPA
                     KNPVMLINPQLRQ"
     gene            14796..15695
                     /locus_tag="PLR47_00395"
     CDS             14796..15695
                     /locus_tag="PLR47_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOG11419.1"
                     /translation="MNTLRKAGIVLAAGALIFCAFVVFAQEGGVGMSDWTKIIESKVT
                     NQVGDKGLINYEDGYIESVGTGAPPERFYGRPQARPMALRAAQVDAFRNLLETVQGVQ
                     IDSQTTVKDFAVESDVIQTAVSGMVKGAQIVKKEYLSDGTVEVTVRMPLSGVVRAVLP
                     KAIADDKKEDREHKPLPFSKRYAPKDEAYTGLVIDGRGLQARPAMSPKIFDEDGAEVY
                     GTLIVNKNYAMQQGICGYARDLTAAQSNSRVTNSPLTVKAVRAQGAGMSEFVISNDDA
                     KLIRSAKQNLAFLQKCRVMVVLD"
     gene            15912..17378
                     /locus_tag="PLR47_00400"
     CDS             15912..17378
                     /locus_tag="PLR47_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOG11420.1"
                     /translation="MIKSIKESPLYSIVNPRSIAFFGASNNFLRMGSIMLSSVQELGY
                     EGKIYPVHQKEKQVRGLTAYRSIMDLPEVPDLAVIVLPTAIVCRTLEECGKKGIRHAI
                     IVSGGFREAGPEGAALQKEMEAVARQYGIRFLGPNCLGIANPYIKLNPTTLTAEGPPG
                     FVGLASQSGSFITQMFDYLHRNGMGFSAALSVGNEASIDIVDCLEYLGVCPNTKVITL
                     YIEGITRGEAFVRAAKNIIRRKPIVALYVGGSEAGKHAGRSHTGSLSGPNEIYDGVFR
                     QCGIIRAQSLTEMFDYALALGTLPKPKGNRVIIQTHSGGPGATAADACGRAGLRLPPL
                     SAQTVEKLQPMIPRTASTANPLDLTYSLNSAESFFDIPDLLLGDPNADMLLAYFLSPG
                     IFVDRILREMGVSEEEIPKTRNKMLIDHAEAFRSLVKRHPDKPIVGFTYRSLQEGMVR
                     YLLDRAIPIYQDPERAARAMAAVLQYHRVRESIATSDV"
     gene            17479..17733
                     /locus_tag="PLR47_00405"
     CDS             17479..17733
                     /locus_tag="PLR47_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11421.1"
                     /translation="MNIDPAAENEKKNRLEMIRQALKDKAPLMYEDLESSGRLQQFLE
                     AHDAEMIASYNEAKSRAWEETRENFLNFTDISYDETSSPM"
     gene            17863..18207
                     /locus_tag="PLR47_00410"
     CDS             17863..18207
                     /locus_tag="PLR47_00410"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOG11422.1"
                     /translation="MKDSIKPRYEARAKILKAMAHPSRLLIIEELQKQERCVNELTEM
                     VGADVSTVSKHLSVLKNAGLVTDEKRANCIYYTLRCTCILDFIGCVEDVLAANAEEHK
                     KIMKSCKNRRLE"
     gene            18204..18437
                     /locus_tag="PLR47_00415"
     CDS             18204..18437
                     /locus_tag="PLR47_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG11423.1"
                     /translation="MKIEILGTGCAKCIKLEELVNEAVKTTGVSAEVWKVKDIKKIMT
                     YGVMTTPGLVVDGQVKIAGKMPTAEQIKSWIKG"
     gene            18575..19015
                     /locus_tag="PLR47_00420"
     CDS             18575..19015
                     /locus_tag="PLR47_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG11424.1"
                     /translation="MTLRLKILGGLAVWAVFTLIFLSLGAGVWAQDKQAAKTDASKER
                     AGANPSVFKPRVTFVEIGAARCIPCKAMQPIMKEVSETYKGQVVVLFHDVWTQQGRMD
                     AEKYNIRVIPTQVFLDINGKEYYRHEGYFPKEGVVEVLKKGGVK"
     gene            19016..19723
                     /locus_tag="PLR47_00425"
     CDS             19016..19723
                     /locus_tag="PLR47_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOG11425.1"
                     /translation="MLVDLFTFLTAALTQSPGIAIGAALIWGILSIILSPCHIACIPL
                     IVGFIDGQGNISTGRAFVLSLFFGLGILITIGMIGLITALVGRMMGDIGGYGSYFVAV
                     VFFAVGLNLLGIMPLPFMEGGANPNYKRKGFLAAFILGLIFGIALGPCTFAYMATMLG
                     VVFSIASTQLTLAMLILAAFAVGHCAVLIFAGTFTEVVQHYLHWTENSKGAVILKKIC
                     GVLVILGGVYLIALNWI"
     gene            19732..20148
                     /locus_tag="PLR47_00430"
     CDS             19732..20148
                     /locus_tag="PLR47_00430"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOG11426.1"
                     /translation="MKKRVLFLCTANSSRSQMAEGIVNHFLGDRLEAFSAGTQASFVN
                     PTAIKAMKEIGVNISHHRSKHLSEFDGQTFHFVITLCGDANETCPLYIGGTKKTHIGF
                     DDPAKATGSPEDILWEFRRVRDEIIDKITNYFLNIA"
     gene            20159..21205
                     /gene="arsB"
                     /locus_tag="PLR47_00435"
     CDS             20159..21205
                     /gene="arsB"
                     /locus_tag="PLR47_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOG11427.1"
                     /translation="MTEHIGQKLSFLDRYLTVWIFAAMILGVGLGYFIPGTEAFINSF
                     QVGTTNIPIAVGLILMMYPPFAKVKYEEMPQVFRNGKVLGLSLVQNWLIGPVLMFFLA
                     IVFLPDKPDYMVGLIMIGLARCIAMVIVWNELAKGNTEYAAGLVAFNSIFQVLFYSVF
                     AWFFITILPPYFGFSGSVVDVSIRQIAESVFIYLGIPCIAGALTRLAGVKVMGKEKYH
                     TRFVPIISPLTLIALLFTIVVMFSLKGNLIIQLPLDVVRIAIPLLIYFVVMFLVSFYM
                     GNKLGADYSKTTTLAFTAASNNFELAIAVAIAVFGINSGAAFAAVIGPLVEVPVMIGL
                     VMVALRFQEKYFQK"
     gene            21224..21847
                     /locus_tag="PLR47_00440"
     CDS             21224..21847
                     /locus_tag="PLR47_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOG11428.1"
                     /translation="MLKLKRFPLLLVSLMGHLTILLTFSGSVTPAYGQTVGQGIPSWG
                     QGPYEVIIFADYFCPPCKRIDAKAEGLFKELVASEKVKLTFVDVPFNRATPTYAKYYL
                     YAVKADAGINNALHVRNVLFEAAQGRRVQDENALVAFLKEKNTAWKAMNEKSVFPHLS
                     AVIKEHKVDATPTCVIKYSAKDIKKYVGDDEIWDGLTKFKAHLWTVK"
     gene            21886..22338
                     /locus_tag="PLR47_00445"
     CDS             21886..22338
                     /locus_tag="PLR47_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOG11429.1"
                     /translation="MRTKGILLIVLSLVMPFSLSAADGVTFENYLKRFDYRERREMKT
                     GIVEMLKLYNQGKVQIIDVRFPEEYKAYSFGFIKNIPLNELPGRLDELDKAKIVVTAC
                     PHYDRAELARTYLKLQGYQSKYLTDGLLGLAEYLRGDKAKDFMFEMKR"
     gene            22403..22615
                     /locus_tag="PLR47_00450"
                     /pseudo
     CDS             22403..22615
                     /locus_tag="PLR47_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258239542.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
     gene            22714..23220
                     /locus_tag="PLR47_00455"
     CDS             22714..23220
                     /locus_tag="PLR47_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG11430.1"
                     /translation="MSENEVTQIRVKGNLVGIVGLKSVIAAMAPDFSRNSDEAIGAEM
                     IKRLGGKNYIPASAKPLYARALVREFRMFLGQPVEEEETGGVLRVIILGPGCANCSSL
                     ENDVRNVLSEMNAPGEMVHVTDPREIAKYGVMGVPALVINQKVASVGTMPEKKKIRQW
                     LEEALKQM"
     gene            23284..>24278
                     /locus_tag="PLR47_00460"
     CDS             23284..>24278
                     /locus_tag="PLR47_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOG11431.1"
                     /translation="MKEWQKLLWIIGIFLAAFYIPWESVLIRQAGLEAFMMLQDYARQ
                     HVLTCLIPAFFIAGAISVFVSQAAVLKYFGAKANKILSYSVASVSGSVLAVCSCTVLP
                     LFAGIYTRGAGIGPATAFLYSGPAINVLAIILTAKVLGWQLGLARAIGAVIFSIVTGL
                     LMAFIFRKDDANRETGEIFLPEDEGKGRALWQDAVYMLVMVLILVFASFAKPSEDDGS
                     AWRMIFSVKWFLVMGLLVVLGIMLKKWFVKDELKGWVQATWDFAKQILPLLFGGVLVA
                     GFLLGRPGYPALIPESYIQMLVGGNSLGANFFASVVGAFMYFATLTEVHERPDYGG"
BASE COUNT         5624 a         6102 c         6788 g         5654 t
ORIGIN      
        1 cgccgaactg gaggaattgc cgaaaggcgc ctacaatata cgaaaaaacg gcaaactgct
       61 ggaccggcat gtatcggcga acatcaatat tgaaaccaac gaagatgaaa ccggcattat
      121 tgtcaaaatc aaaccaggaa cgaaaaatga atcggtccat attcccgtga tcttaagcca
      181 ggccggcctg tatgacgtcg tgcagaatgc ctttgtcatc ggcgacgacg cggaagtgac
      241 gattattgcc ggctgcggga ttcactgcgg ctcatccgaa ccggaagggc acgcgggaat
      301 tcacgaattc aaggtcggga aaaatgcgaa ggtgacgtac gtggaaaaac actacgcgtc
      361 cggcgaaggc acgggacgcc gatcgctgaa tccgacgacc aaggtgtttc tggccgaggg
      421 ggcgcgcgcg gaaatggaat tgacgcagat cggcggggtg gatgataccc accgtgtcaa
      481 tgaagcggtc cttggtccga acagcctttt gctcattacg gagagggttc tgaccgacgg
      541 cgaacaaacc gccgtgtcca ccaatgatat cgtgatgaag ggcaggggaa gccgggccaa
      601 catgatttcg cgttcggtga tgaagggcaa ttccaaacag acgttttacg cgaccatcga
      661 cgcgcagaac cagtgctacg gtcacattga gtgcgacgcg atcatcatgg acaacggcac
      721 caacgaaacc atgccttccc tgaaggccgg ccatcccgac gcggaattga cccatgaagc
      781 ttcgatcggc aagatcgcga atgaccagct gaccaaactg atgagcctgg ggctgaccta
      841 cgatcaggcc gtaaaccgga tcattcaggg cttcctgaaa tagccgggac ccgaaaaaac
      901 gtattgcctg aatttgcagc atcttctata ataccgcgca accacgatca tccgccaaag
      961 ggggtattca tggataaaag aatgaacgca ctggcctttg ctttggaaaa cgaagaaaag
     1021 gagcgcgagt tttatcttgc ccatgcccgg aagaccagga acatggccgg gaaaaacatg
     1081 ttcaagcaga ttgccgacga ggagaaagag cattacgaaa tgctcaaaaa actccacgcg
     1141 aagtgggagg aaaagaagaa atggccggcc acgatcgccc tgaaagtccg gccgtctgcc
     1201 gccggagcca tcctgcaggg catgtccggg aaaaaatcag cccgcattgc cggaacggat
     1261 gatgagctca aggcggtccg cacggcaatt gattttgaag cgcgcggcgt ggccctttat
     1321 accaagctgg ccaagcagtc cacggatgcc aaagagatcg cgtttttcaa tatgcttgcc
     1381 tccgtggagc ggcagcacct gctgtcgctg aaggacacgg aagaattcct ggccgatccg
     1441 gccacctggt atcagcacat ggaacgctcg ggtttggacg gcgcgtaaga agaaccactg
     1501 cgcgcaggga atggattaaa accgtttcct gcaaatccat gaaggaaatc aacgacgcca
     1561 aaaagggaac gcttgatttt gatttggaat caggcgaagc cgtctgaatc agtctcttcc
     1621 agtttgcaac tcagttccgt ccacaggtgg taggcttgtg caagctcgtt ttcaacggcc
     1681 ttcagatcga tctgaaggcc tttgattttc actgcgtctt tgtgtgtttg gggatcacac
     1741 agggaatgtt catcgccagc ctttcgggat tcaagctctg cgattttttc ttccaacttc
     1801 gccagctccc tctttaagcc gtttttgatt ttcgacaggc gattgcgttc ttccgcttcc
     1861 agtctttttt tgtcttttgt tttaagcgtc ctgtccacag gcgcccggtc gcggcgctcc
     1921 tgctgtccgg catccgccgc catccgggcg cgcttttcaa taaagtagga ataattgccc
     1981 gcgtaatccg tgacggatcc gtcctttagt tcaaagaccc ggttgaccag acggtccaga
     2041 aagtaacggt cgtgggaaac aatggccatt gtgccggaat agtggatcag ggcgctttga
     2101 aaaacgtctt tggttttgat gtccagatga ttggtcggtt cgtccagcac caggaagttg
     2161 gtgtcggtga gcatgatttt caaaagcgcc aggcgggatt tttctccgcc ggacaggacg
     2221 ccgacggact tgtaaatatc gtctcccgaa aaaagaaaag ctcccagcag gtttcgcttg
     2281 ttctggtcgt cggcggacgt cggaactccc tgcacttctt cccagatggt gttggcgtag
     2341 ttcaggttct gcgcgctttc ctgagagaaa aaagccatgt tgacgccttc tccatacgtc
     2401 accgatccgc ggacaggcct ttcgaaaagg ctcaaaagcc tagacaacgt ggatttgccc
     2461 gatccgttca cgccgacgaa ggccaccttg tcaccccgaa aaagcgaaaa attcaaaccg
     2521 gaaaaaaccg ccaggtctcc ataggcatgg cccagatcgc agaccgtgac cacttccttt
     2581 gcgctttttt tgccttccgg gaatttcatg acgacgcttt tggcggcgcc ttctgcctga
     2641 aggtctttaa atttttccag catcttgacg cggctttgca cctgcgacgc cttggtggcc
     2701 ttgtagcgga accgctccac aaacgattca atttttttaa tctgcgcttt ttgcagttcc
     2761 atttcttttt tcagcgcttc aaaccggcgg tctttttcgg caagataata ggtgtagttg
     2821 cccttgtaaa tgtggatgcg gcgattggct aattccgcga tttgcgtggc gatcttgtcc
     2881 aggaaaaaat gatcgtggga aacaatcaga atggttccgg ggtagtcctt caggtagctt
     2941 tccagccatt ccatgctctc cgtgtcgaga tgattggtcg gctcgtcgag cagcatgatg
     3001 tccggccggc ccagcaggat tgaggtcagg agaatccgca ttttccagcc gccggaaaac
     3061 acgtcgcagg ttttttcaaa atcctggggc cggaagccga accctttgag aatctgtttg
     3121 gctcgtgctt caaaagcata tccctcgcgg gcggaaaatt cggcggttgc ctgcgcataa
     3181 cggcttgaaa gttcgccgta ttcgcgcgaa tgggaaggcg cctgcgccat ttgccgctcc
     3241 agagagcgga ggtcttcctc cagcccggca atgccgcttt tccggcgaag atactcgatc
     3301 aggcctgtcg attcgagttc gaccaggtcc tgcggaagat agccgatctt tttttgcttc
     3361 cggccgggga tttcgataag accggaatcg agaggcacct cgccgagaat ggcccgaaac
     3421 agggtcgttt tgcccgcgcc gttgtcgccc accaggccga tgcggctttt gggatgaatc
     3481 gtccattgaa tccggtcgaa cagcgttttg tccagaaaag acaaactggc attgcggaaa
     3541 taaatcaccc acagcactcc gttttcaagt ctgcgggact ataggaaaat aaccttccgg
     3601 tgtcaacgtc tttccgtaaa agcctccggg tttgcctgtt ccagccaggg attcgtgttg
     3661 atgttttctc cccagaagat tttgggatcc ggaggcgaag ccccccacca gttgttgcgg
     3721 gcgtcgatga aaatcgacga aaaactctga atggccacgg cgttgtcgat gaagttgttt
     3781 tgaaaaatct tgggattggc cgaaccgacg catttgaccg cgccttcccc ctgattgtgc
     3841 gcaaacgtgt tgtgggaaag gaccggggcg gcgtctttgc ggcagtaaac gccgttctgc
     3901 gaggcgtggg cgatgtaact gtgggagatt tccggcgatc ccgtgtaaat gtcaaaggcg
     3961 gttgtcgcgt acttcacgat gcaataggca aaggcgctgt tgatttttgt ggcttcggaa
     4021 aagcgcacgg cgctgacata gaagcccgga gcgggtgatg cggaagcggc ggtgaaggta
     4081 atcggctttt ccccggttcc cttcgcgacg atgccgccgt ttctggtgat gaaggaggtt
     4141 tcctgttcgt attcgatgac aaccccgggt tcgatgtaga gagtggcgcc tccgtcaatc
     4201 accacgtctt tgaccaggcg atagggactt cgcgataaaa tcagataggc atccgcctgg
     4261 atcgcgcctt ccagcttctg atccagaatc ggaacggcca gcgctttgcc ctgcgggtac
     4321 ggggcgtcca gcacggagct gacatcgacg cgtccgtgta ttccggacag aacggccgcc
     4381 atggcggcgc tgccccacca gttgttcagc gcctttgcgg gctctccttt catttcgccg
     4441 aacacattgt agggctgatt gtccaccagg ttgttttgaa agatgaccgc ttcggcgcct
     4501 ttgaccgcga tgccgttgtt tttgttccgg gtaatcgtgt tttgcgtgat ttccgccgcc
     4561 gcggagtgca gcaccactcc cgtatggtcg ttgtcgttga tggtgtttcc cacaatgcgc
     4621 ggcgcggctc cgtcgcttac ggaaatggcg gcgcccttgt ttttgtaaag ggtgtttcgg
     4681 ctgaccagcg gcttggaaaa gactccctgg attttgatcg cttcgtggtt tttcgtgaat
     4741 tcggattgct caatggtcgg cgaagaggcc tgacaaaaga cggcggtccg cgcgttttgc
     4801 acgcggacga acgccagatg gttttcccgg tccttcacgc tggcaaaggt gatgcccggc
     4861 cagaactgac cgtcctgcgc cgaatcaaaa cggatgatgc ggtcattgtc gccccgggct
     4921 gaaatccgtc cttcgatcac gagcccgccg cctttggact gaatttccgt tcccggttca
     4981 atggtcaaaa gcgccttgtc cttcacggtg acattggatt ccagaatgta ggggctggcg
     5041 cccgaatacc aggtggcatc cgcctcgatc gctccggaaa ccggcgtcgg ccccggtgcg
     5101 atcggcatgc cctcggcctg ctccgtccgg ctttcattgc ctgcaaaatc cacggccgaa
     5161 atccggtaat aataggtccg ggcgtttgcc agattctgat cggtgtcctt gtactcgttc
     5221 agttccgttt tgccgatttg aaggtatccg gaaagcgggg tctcggagcg gtaaatgaga
     5281 taaccggcca gatccgccgc ggtgtttttc tcccagcgca gcgccacccg ctggtttctt
     5341 cccgttgatg taatgcccgc gggtttgtcc ggcgcggtgg tgtccagcgt gacggatccg
     5401 atcgcgtcca cccagtgggc ggtgtttccg gaatcgtccg tgagaaagcc ggtgacgatg
     5461 gctttttcga tgttgtcccc gggcaccact ttgtaaacgc cgtaataacc gcccggctcg
     5521 acttcctgca tttcgatgcg cttccggtac tggccgatgt caaaagaagc ctgcatgtta
     5581 ggcgttccct gaatgaccac cttgatctca tcgcctgctt ttttcggcag tcctttggtg
     5641 tcctgcgtaa ggagcgtaat gaccggcgga cgcttggcct gagccagaga cggcgtggga
     5701 atggttttga ccatgtcgcg gaacagatca tcgttggcgc gcaggagctg aatgtcgcgg
     5761 acattcaggg cggtcgcaat taccgtcgcg atgatgccga ccggattcgt ggaaacgccg
     5821 ccttcgtgct tgcgcacttt atgttttccg ctccacagaa agttgccggt tttggcgtcg
     5881 tacattttca cttccgcgcc cacggcaacc tgcgaataga ggaccgcgaa atatttgtca
     5941 aaatcggaaa cctctccgaa aacaaccgcg tcaacgccca gaatttctcc cagctttcgc
     6001 ggagggattt tgcggatgac atccgggtcg gtcaagcccg ccttcaaaag cagatcgtcc
     6061 accttgtaaa gttccatgtc tttgaaggga agggagctga aatggttgta gaagcctttg
     6121 cgcatggcgt ttgccccttc cttgctgccg gacagatcga caaagggcag aacggcaacg
     6181 gaaaggggaa catgtttttt catgtaggga tcgacggtat attccccttc atacaaagac
     6241 cggatttccg gcgtaatgag cgtggatggc gtggagacga ggcagccctg cagcatcaga
     6301 gccgcggccg gaatcagacc ccaaaagact actttgaata tttttcgcac ggtcaaacct
     6361 ccatcctgac gggatttttt ggaattataa catacgtccg gccggaattc attttaaaat
     6421 tctttcgcaa atttgccgcg ctaaaaagcg tttgtgccgg atgcatcaag cggttcccgg
     6481 cgggctcagg caggtccggc tggcgatgaa cgaataataa tccgaaaagg cgagctgcca
     6541 gggctgcatg gttttgccgg ttgcggccac gaacttgtgc atgccgagca cgctgtaagc
     6601 cggtcgtcgg gccgcccgct gcagttcgct ggttttcatc ggggacaatt cgacctgctc
     6661 catgcgggct tccttcagga ttttgcgcgc aaaatcgtac caggtgcagc agccgcggtt
     6721 ggttacgtgg aaaatgccgc gggcgttttt gtccaccaga agctccgtcg ccgccgccag
     6781 gtcgcgcgtg caggtaggcg agcccatctg atcgtcaaca accgtcagtt ttcccgtctc
     6841 cctggcccgg tccagaatgg ccgaaacgaa gtttttcccc tttgcgccgt aaagccaggc
     6901 ggtccggatc agaataaaat tgtccgtcag acgcttcaga tggtgctcgc ccgccagttt
     6961 ggaggcgccg taagtgttga tcggattgca gggatcttct tccgcgtaag ggtgattcgc
     7021 gtttccgtca aaaacgtagt ccgtgctgta atgaatcatg ggaacattgc cgtcccggca
     7081 ggcctgggcg atgtttttga ccgcttccgc gtttaccgcg aaacattctt ccttggcggt
     7141 ttcgcaggcg tccacattgg tgtagccgnn nnnnnnnnat gccggtgtct gaattgagcc
     7201 atcagatcgt ttcccagcat tcccttgtgt cccagcagca gtactttcat gtgttcagta
     7261 tttcacctcc tttaagaaaa ggccgcaggc gggcgcggct gttccggcct gtttccggtc
     7321 gcgcgaggcg aggatttgcg gcatgctttc cggcgcgatt tttccccggc ccacgtccac
     7381 cagcgtgccg acgatgttgc gcaccatatg gcgcaaaaat ccgctcgatt caagcgtgat
     7441 ttccagccgt ccgctttcct gatggacaat cgagcagacc gtgagaagcc tgacgcgatt
     7501 cttaatatca cagcccgtcg cgcaaaaaca cgaaaaatcg tgttcaccga tcaatttcat
     7561 cgcggccagg cgcatgctct cgaggttcag cgggcagcgc acgtgccaga aatactggcg
     7621 tcccagcgcg gggcgcagac gctgattttg tatgcgatac acataaacct tgcctctcgc
     7681 atctttcagt gcgttgaaat ccggcggcgc ttcctgcaaa tccttcacga caatgttgtc
     7741 cggcaaaacg ctgttgacgc ccaaaaaaat tttgttggcc gggagaacgg tgcgcgaccg
     7801 aaagctggcc gcctgattca tggcatgcac gcccgcgtcc gttcgtccgg aggcgatgac
     7861 cgtcgcgttt tcgccgatga tgcgcgccag cgcctcttcc actacctgct gaatggaaag
     7921 cccgttttgc tgcctttgcc agccgcagta ggctgcgccg tcatattcga gaatcatttt
     7981 gaagttgcgc atatcgcaca aatcagactt gccagtttgc cccaaaaagg ttaagcactt
     8041 cttcgccgtt ttcaaaacac atggccgggc cggtattttt tcgtccggga tagtaacgca
     8101 ggcccgaagc ccggtcaaga taaaacggaa aacggtttat cgaatcagga aattacgaag
     8161 gagatcagtc atgggggacg atatcaggca ggcgacgacg gaagggaaaa ttcgggactt
     8221 tgaaacccgg gcccagtcga tgatgaagat gggcggggaa aagcaggtgc aaaagcagca
     8281 cgacggcggg aagctcacgg cccgtgaacg gttgaaccgg ttgtttgatg aaggcagctt
     8341 tcaggaagtg cagcttttcg tgaagcatca cgccaccctg ttcggcatgg acaaaaagga
     8401 aattccggcc gacggcgtca tcacgggctt cggcaaagtc aacggccgga ttgtcttcgc
     8461 cgccgcccag gattttacca gcgcgggcgg aaccctgggc gaaatgcacg ccaaaaagat
     8521 atggaaagtt atggacatgg cgctggacgc gcaaaagccg tttgtttcca tcaacgattc
     8581 cggcggggcg cgcattcagg agggcgtgcc gtctctggaa ggctacggcg gcatcttcta
     8641 ccgcaacacg ctggcctccg gttatattcc ccagatcacg gcgatcatgg gacccacggc
     8701 cggcggcgcc gtgtattctc cggcgctcac cgactggatt ttcatggtga agaatacatc
     8761 gtacatgtac atcacggggc cggacgtgat caaggccgtg attggagaag aagtttcgca
     8821 ggaagacctg ggcggagccg tggctcacgc ctccaaaagc ggcgtgtgcc atgtcgtgac
     8881 tgaaaacgac gaagactgca ttcaaaaaat caaaggattg ctgtcgtacc tgccggacag
     8941 ctgtcattcg ccgctgccgg tcgcagagac gaccgacaac ccggaccggg agtgcccgga
     9001 attgaacaag atcattcccg acagagcggc gcgcgcctac aatatgaaaa gcatcatcaa
     9061 atcggttgcc gacaacggcg aatggtttga gctccacgcg cagtgggcgc cgaacatcat
     9121 cgtcgctttg atccgcatca tgggccggcc cgtgggagtc atagccaaca atcccatgtc
     9181 ttatgcgggt gttctcaacg tcaatgcttc ggacaaggcg tcgcgcttca tccgtttctg
     9241 cgacgccttc aacattccgc tcgtcacgtt ctccgacgtt cccggctaca tgcccggcac
     9301 ggaccaggaa tgggcgggca ttatccgcca cggcgcgaag cttctgcacg cctattcgga
     9361 agccacggtg ccgaagatca ccgtcgtcac gcgcaaggct tacggcgggt cctatatcgg
     9421 catgtgctgc aagcagctgg gggcggatta cgttatggcc tggccgacgg cggaaatcgc
     9481 cgtgatgggg gcttcgggcg cctgcaacat cattcaccgc gccgaaatca aaggggctgt
     9541 ggacccggat gcgaagcgcg cggcgctgat cgcggattac gaggaaaaat tcaataatcc
     9601 ttactatgcc gcatcgctgg gtgctatcga ggaggtgatt ctgcccagcc ggacgcgcaa
     9661 acgcattgtc gtcctgctgg aggctctgca gaataaaaag gaagcgaggc ggacaaagaa
     9721 acacaacaat attccgttgt gaatggtccg acggacgtct tgaaaaagcg gggaagattc
     9781 gagagacttc cctgcttttt tgcgtttaat gccggacgaa aatcttgaac cgattgaaaa
     9841 atatcctgtt tttgcgtttc cagtgattat tcctgttgtt ttcttaatat taccggcaat
     9901 tacaaataat cataatattt cctttacaaa aacagacgca agtgctaaat gacctccagt
     9961 cacccgaagg ccgtgcggaa tttgttgcgg cggctgtctt taaaatagag ggtgaaactg
    10021 tgaataagaa gtgtgcaatc gcatgtaaaa acctgaactt caggataaat catatacagg
    10081 aggagatcat gaagagattt tggttggttt tactgtcgct ggggctgatc gtagccttca
    10141 gttcgacggc gatggccgtt gacgtaaagt tcagcggcga gttttacgcg ggtggaatgt
    10201 atctggataa aacaaacttt accaagtcca caacgggcgg aaatccgagc acggctttct
    10261 attaccagag attgagggcc agaacggact ttaccattac ccccggcctg aaattgatta
    10321 cgcgtttcga cgcgatggag cgggcctggg gcgcggcaag atccaatccc gttggtcccc
    10381 tggctgcaga ttcggcggga acggtggcgg aaaatgaaaa cattgccatc gattgggctt
    10441 acattgatta caaatcgccc atcggcactt tcctggtcgg ttacatgaat tatggaacca
    10501 cgggaacggt ttttggcaac aacagaaata ctcaggccag aatccggttc aacagaatgt
    10561 tcggcccggt taatgtgtgg gcttctattt caaaagtggc agaaaacagc ttgacggccg
    10621 tgaaacctgc ttcaacaaca tcggatgccg ataatgacgt gtatcatctg gaaggcattt
    10681 acaactggaa aagcggccgt gccggtttga atgtcaacta ctacaattat gccaatacac
    10741 ggcccgcgcc gacttcttac aagagaacct atttcctgtt taccccttat gtgttcgctc
    10801 aaatcggtcc cgtgtccctg caggccgaat tcaattacct caacggcacg gacaagtatg
    10861 aagatggtgt gacgacaaca gtggacaatc tggacaaggt gacgctggaa aacatttccg
    10921 cctacattga cgcgacggcc aatttcggca tgttttacgc gggcggcagc ttcgcgtatg
    10981 tgtccgggaa cgatcccgga acgaacgaca aaaacgaggg cggactcatc aacggcgggt
    11041 atgactggaa tccctgcctc atcatgttta actatgacga tcgggccaag tgggccggca
    11101 acctcacggg ctacggaacc agcgccgata acggcagaat ggcgaacgca tggttcttcc
    11161 agttgagggg cggcgtgaag ccgatcgaca agctggatgt catggcgtcc gtttcctacg
    11221 ccaacgccga caagaaacct gcaaactatc tttacaatga ttacggatgg gaagtggatt
    11281 tgaccgcaaa ttacaaaatc accaacaacc tgtcctacat ggtgggcgcc ggctatctgt
    11341 ttaccggcaa gtactacaag gccaatgtgg ccgtcaacaa cgagttgaac gacaatttcc
    11401 tcgtcatcaa caagctgacg ctgacgttct annnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn naatgaattt caagatcgtc ctgctcatcg tggttgcctg tttggccgtc
    11581 gtttttgtca tccagaatgt gtcggccgtg acggtcagca tttttttctg ggaaatttct
    11641 ctgtctcttg ccctccttat tttctttgtc ctggcctttg gttttgcctt cggctggttt
    11701 ttgcacagtt ttctggcgta ccaaaagata aaaaaagaag tgaaagacat tcaaaacgat
    11761 atgcgcttgc agaaatgaca agacggcgat ttgatcaacc ccttacagtc tgacggactg
    11821 caaggcttat cccttcatcc actcttttcc gcccagcact cgagccacca tcatactgga
    11881 aacgttgtcg cctgtggcat tgaccattgt ggcgggcgcg tccaccagcg tgccgatcat
    11941 cgtgatgagc ggaaacgctt ccggcggaaa accgtacagg gataaaatca agagctcgcc
    12001 gatcgtgccg ccgccgggaa tgccgctcac caccacgccg gtcaaaagcg cgatgcccag
    12061 agcggtcaga atcgtctcaa tgccggcaaa aggcatctga aaaatgccga agagaaaggc
    12121 aatcttcaaa acagcggcca ggcacgatcc ctccatatgc atcgtcgcgc cgatgggaat
    12181 cacgacttcg ctgatgtctt tcggcacgcc gatcttgtcc gcggcctgca gattggcggg
    12241 aatggttgcc atcgaactgc cggtggccag cgccgtaacg gccggcggaa taatatgggt
    12301 ccagaatttc tttacgccgt aggcccggcc ggcgataaaa gcatacaaag taaaggcaag
    12361 gaaaaaataa ccaatggcgg tcggatagta aacggccatc gcccgtgcgt aggagcccag
    12421 aagctccggt ccgaaaacgc cgaccaggta ggcgaaatag gcgcacaggc cgatcggcgc
    12481 gtaatacatg atgaaagaga tcagcttcat catgaccgtg ttggccgaag cgaggaacct
    12541 cgagaagtct tttccctttt ctcccgcggc ggacgcggcc agtccgacca gcatcgagaa
    12601 gataatcagt ggcagcatat gattcttgga taaaatatgc gggaaatccg gaacggtaaa
    12661 ggcccgaacc atttgatcgc ctgccttgat ctgctgaaga tctgctgttg ccggcagggg
    12721 gatgtttaca ccggatgcag gaggataaaa aatgacggcg gcgatcatga ccgccgacgc
    12781 catgatgccg gtgatgacaa atatgaggat cattacggac agaatttttc ccaggcgctt
    12841 gagattggtc atcgaagcga cggtggacga aatggaaaag aagaccagcg gcacaatgac
    12901 ggtaaacagc aggttcagaa agatgtcgcc caacggctta agccggacgg cttccttctg
    12961 atagatcatg ccgaaaatag cgccgagaat gatcgaggcg atcagcagaa tcgagaaccc
    13021 gtaagatttc aaaaacggcg gcaaatagtt tttcattttt tactttaccg gaaagttgaa
    13081 atacgtgtcc gggtaaggct cgttgtccag tgtgaaatgc caccactctt ccggcaggtt
    13141 tcgaaagccg tgcctggcca tcagcgtttt taaaagcagg cggttggccc tctgctgcgg
    13201 gccgatatct ttgttttcgg gatgggacat ctctccaaag cagtcaaatc ccgtgcccat
    13261 gtccagagag ttgtccgcga agcggacgcc tgccggaagg caacactcct tgaggggatc
    13321 gccggcttta tacgcgggct gaatcggggc gggcagcggc acaagggtca ggtcaaccgt
    13381 actcccccgg ctgtggccgg agcgctccgc aatgtagccg tcgcggaaaa ggttctgttt
    13441 gtccacggtg ggataaaact cttttttggt cctggtgtcg ccgacgtctt tcgcccagcg
    13501 cacaaagtgg tcgacggccc tttgcggccg gtagcagtca tagattttga tggtcattcc
    13561 aaaaggcgcg aggtctttct ggacccgggc cagcgcctct gccgccggac gggtcagaat
    13621 gcacgcgggc gcaaggtagc cgtccacttt gacgcccaga aaattatgct cggtaaagta
    13681 gcggatatcc agaagggcgt cggggatgat ttcttcaatg tggacgaacg tgtcgggcat
    13741 ggcgtcttcg cacaaggatg cggggggcgc cgccgctgaa aaccagatcg cggcgagcag
    13801 gccgatcagg aaaaattttg acttcatgaa gaagacctcc ttattgccgt aattgagggt
    13861 tgatcagcat gacgggattt ttcgccggat tcaaccagcc gagcattttc agaatgtcgt
    13921 cttccgatgc ggcgacgcat ccggccgtag tggaatccgt gcccgcccag acatgcagaa
    13981 aaatcgcgct gccgaatcct ttgatgaccg ggtccgtgtt gtattcaatc acgatcccgt
    14041 atttgtatag atcatcatcg cgtttcattc tttcgtagga agccgccttc gtctggtttt
    14101 gatggaccca gcggttgtaa tccggatcgt tcgggtcatc gatccacaga tcattctcct
    14161 gagactgacg gtagggcatt cgagtgggaa cggacggagg ataaccaaaa gcggttttca
    14221 ttcggaagag ccccgaaggc gtgcgtccgt ccccctcgcg tttttcgccg ggcggcgcga
    14281 agccgtttct gccgatgacg gcatccaagg ggttcttggc ctgccgccag gaggagtccg
    14341 tcttctccag ggggtaaatc ttatgagaag tcaggaaaag aaagctttca tccgtgacca
    14401 gaagaacctg cccgacattt tccatcgccg cggtttgaac ataaggcgca agccggtccg
    14461 tcacggcggt gcgcgacgcg caggcggcaa gcgaccaaag ggctgccagc gcaagcaggc
    14521 aaccaattcc ggtcaatgat ctccccgatt tcccgcaaaa tagaatcatg agcggatttg
    14581 taatggaaag ttttttattt ttcaatcaat ttgtgacgtt gaatcgaggc ggccttcttc
    14641 ttttttgccg gccgtggtcg attggggaag cggctgatga tgacggagcg tcctttcggg
    14701 aggaaaaaaa tcattttttg gctcaaatta tggtataaag ctatcacatc tacaaaagat
    14761 gaacagtccc gattgaaaat aagaggaggt tgaacatgaa tacgttgcgt aaagcgggaa
    14821 tcgtactggc ggcaggcgca ctgattttct gcgcctttgt tgtctttgcc caggagggcg
    14881 gggtcggcat gtcggactgg acaaaaatca ttgaatccaa ggtcaccaat caggtgggag
    14941 acaaggggct gatcaactat gaggacgggt acatcgaatc cgtgggaacc ggcgcgccgc
    15001 cggagcgttt ttacggaagg cctcaggcgc ggcccatggc gctgcgagcg gcgcaggtcg
    15061 atgccttcag aaacctgctg gaaaccgttc agggggttca aatcgattcc cagacgaccg
    15121 ttaaggactt tgccgtggag agcgacgtga tccagacggc tgtttccggc atggtgaagg
    15181 gcgcgcagat tgtaaaaaag gaatatctat cggatggaac cgtggaagtg accgtgcgca
    15241 tgccgctttc aggcgtcgtc agggccgttc tgcccaaggc gatcgccgat gacaaaaagg
    15301 aagaccggga gcacaagccg ctgccttttt ccaaaaggta cgcgccgaag gatgaagcct
    15361 ataccggcct ggtgatcgac ggccggggcc tgcaggcccg cccggccatg tcgccgaaga
    15421 tttttgatga agacggcgcg gaagtttacg gaacgctgat cgtgaacaaa aactacgcca
    15481 tgcagcaggg catctgcggt tacgcccgcg acctgaccgc cgcgcaaagc aactccaggg
    15541 taacgaacag tccgctgacc gtcaaggccg tccgcgctca gggcgccggg atgtcggaat
    15601 tcgtcatcag caatgatgac gctaaattga tccgttccgc gaaacagaat ctggccttcc
    15661 tgcagaagtg ccgcgtcatg gttgttctgg actgatttcc gggtttcaga agatcatcgg
    15721 tttcaatgaa gggccgcttt gtccttgcgc gaagcggccc ttttttatct cctggttcat
    15781 gtgttcttga attgcgggga attatctggt aaggacagcg gcaatatttg aaggcaagcc
    15841 ttgattttga ggaggaatct ttttttcagg gaaatactgc aatccttaca gccgcgatcc
    15901 ggaaggaaga aatgatcaaa tccatcaaag aaagtccttt gtattccatc gtcaatccca
    15961 ggagcatcgc tttttttgga gcgtccaaca attttctgcg catgggatcc atcatgcttt
    16021 cctcagttca ggaactgggg tatgaaggca aaatctaccc cgttcatcag aaagagaaac
    16081 aggtgcgcgg gcttacggca taccgcagca tcatggatct gccggaagtg ccggatctgg
    16141 ccgtgatcgt tttgccgacc gccatcgtct gccgcacact ggaagagtgc ggcaaaaaag
    16201 gcatccggca cgccattatc gtttccggag gatttcgcga agcgggcccg gaaggcgccg
    16261 cgctgcaaaa ggagatggaa gccgtcgccc ggcaatacgg catccgtttt ctcggaccga
    16321 actgtctggg gattgccaat ccctacatca aactcaatcc gacaaccctt acggcggaag
    16381 gtcctcccgg ttttgtgggt ttggcatccc agagcggaag cttcatcacc cagatgtttg
    16441 attatctgca ccgcaacgga atgggattca gtgcggcgct cagcgtgggg aacgaagcca
    16501 gcatcgacat tgtcgattgt ctggaatacc tgggcgtctg cccgaacacg aaagtcatca
    16561 cgctgtatat tgaaggcatc acccgcgggg aggcctttgt cagagcggcg aaaaatatca
    16621 tacggcgcaa acccatcgtg gccctgtacg tgggcgggtc cgaggcgggc aaacacgcgg
    16681 gccgatctca caccggctct ctttcggggc ccaacgaaat ttatgacggc gtgtttcggc
    16741 agtgcggcat catccgggcg cagagcctca cggagatgtt cgactacgcg ctggccctgg
    16801 ggacgcttcc gaaaccgaaa ggcaaccgcg tgatcattca gacgcattcc ggcggtcccg
    16861 gcgccacggc ggccgatgcc tgcgggcggg cggggcttcg gctgccgccg ctttcggccc
    16921 aaaccgtaga aaagcttcag ccgatgattc cccgaaccgc cagtacggca aacccgctcg
    16981 atctgacgta cagcctgaac tcggccgaaa gtttttttga cattccggat cttctgcttg
    17041 gcgacccgaa cgccgatatg ctgctggcgt atttcctgtc tcccgggatt tttgtggatc
    17101 gcatcctgag agaaatggga gtgtctgaag aggagattcc aaagacccgg aacaagatgc
    17161 tcatcgatca cgcggaggcg tttcgcagcc tggtcaagcg gcatccggac aagccgattg
    17221 tcggatttac ctaccgcagc cttcaggagg ggatggttcg ttatctgctg gaccgcgcga
    17281 ttcccattta tcaggaccct gagcgcgccg cgcgggcgat ggccgcggta ttgcagtatc
    17341 atcgggtccg ggaaagcatt gcaacaagcg atgtttgaaa ttctgtttca ggcgttgcat
    17401 aattgtgatg catcatgccc gcaaaatgaa attgccgcag gaagaatggg catagaactt
    17461 ccataaagga tgcaaaccat gaacatagat ccggcagcgg aaaacgaaaa aaagaaccgt
    17521 ctggagatga tccggcaggc cttgaaggac aaggcgcccc tgatgtatga agacctggag
    17581 tcttccggcc ggcttcaaca gtttctggaa gctcacgatg cggaaatgat cgccagctac
    17641 aacgaggcga aaagcagggc atgggaagaa accagggaaa acttcctgaa ttttactgat
    17701 atttcctacg atgaaacctc atcgccgatg taaccggggc attgagcccc ggttgccggc
    17761 gccggacctt tatccattta aaaatctctc cactcaaaat taagccttga ctcattatgg
    17821 ctatttggct atatggccaa atacgaatgg agggaacgtc tgatgaaaga ttccatcaag
    17881 ccccgttatg aagcccgggc aaaaattctc aaggcgatgg cgcatccgag tcgtctgctg
    17941 atcattgaag agctgcaaaa gcaggaacgc tgtgtgaacg agctgaccga gatggtcggc
    18001 gcggatgttt cgacggtgtc caagcattta agcgtgctga aaaacgcagg gttggtgacg
    18061 gatgaaaaga gggcaaactg catttactac acgcttcgct gcacctgcat tctggatttc
    18121 atcggctgtg tcgaagatgt gctggccgcc aacgcagaag aacataaaaa aataatgaaa
    18181 tcctgcaaaa ataggaggtt ggaatgaaga ttgaaatatt gggaaccggc tgtgcaaaat
    18241 gtataaaact tgaagaactg gtgaacgagg ccgttaaaac aaccggcgtt tcagccgagg
    18301 tgtggaaggt aaaggacatt aaaaagatca tgacctacgg cgtaatgacg acgcccggcc
    18361 tggtcgtgga cggccaggtg aaaattgcgg gtaaaatgcc gacagcggag cagatcaaaa
    18421 gctggatcaa ggggtaggcg gccgtccgat taagtaattg ctgtggatgt gtttttttca
    18481 aagcaaccct gtgttcctga catcctattg tatttcagga ttcatattta aaggatatga
    18541 tggatcagac taaaataaaa tcagggggaa atctatgact ttgcgtttaa aaatcttggg
    18601 gggactggcg gtatgggcgg tttttacact gatctttctt tcactgggcg cgggggtatg
    18661 ggctcaggat aaacaggcag ccaaaacaga cgcatccaaa gaacgagcgg gggccaaccc
    18721 ttctgttttc aaaccccgtg tgacgtttgt agaaatcggt gccgccagat gtattccctg
    18781 caaggccatg cagcccatta tgaaagaagt gtcggagaca tacaagggac aggtggttgt
    18841 tctttttcac gatgtctgga cgcagcaagg gaggatggac gccgagaaat ataatattcg
    18901 cgttattcct actcaggtat tcctggatat caacggaaag gaatactaca ggcatgaagg
    18961 gtatttcccc aaggaaggcg ttgtggaggt tttgaaaaag ggaggggtca aataaatgct
    19021 ggttgacctc tttacgtttt tgacggcggc gctcacgcag tcgccaggca tcgccatcgg
    19081 tgcggcgttg atttggggca ttctgtccat aattctttct ccctgccata ttgcctgtat
    19141 tcctctgatt gtcggcttta tcgacggtca ggggaatatc agcacgggcc gggcctttgt
    19201 cctgtctttg tttttcggcc tcggcattct cattacgatt ggcatgatcg gtctgatcac
    19261 ggcgctggtg ggccgaatga tgggggatat cggcggttac ggaagctatt ttgtagccgt
    19321 ggttttcttc gccgttggtt tgaatctgct ggggattatg ccgttgcctt tcatggaagg
    19381 cggtgcaaac ccgaattata aaaggaaagg atttctcgcg gcgtttattc tgggcttgat
    19441 cttcggcatt gcgctgggcc cttgcacgtt cgcctatatg gcgacaatgc tgggcgtagt
    19501 atttagtata gcgtcaacac aattgactct ggcgatgttg attctggcgg catttgccgt
    19561 cggccactgt gccgtgctga tttttgcggg aacgtttacg gaagtcgtgc agcattacct
    19621 gcattggacg gagaattcca aaggtgcggt cattctgaaa aagatctgtg gtgtgctggt
    19681 gattctgggc ggcgtttatt tgattgcgtt gaactggatt taggagattt tgtgaagaaa
    19741 cgtgttttat ttctatgcac tgccaattcc agccggtctc aaatggccga aggcattgta
    19801 aatcattttt tgggcgaccg actggaagct ttttccgcgg gaacgcaggc gtcgtttgtg
    19861 aatccgacgg ccataaaggc gatgaaggaa atcggcgtga atatctcaca tcatcgctca
    19921 aagcatttgt cagagtttga cggacagaca ttccattttg taattaccct gtgcggggac
    19981 gccaacgaaa cctgtccgct ttacatcggc ggaacgaaaa aaacgcatat cggttttgac
    20041 gacccggcca aagccaccgg aagcccggaa gacatactgt gggaattccg gcgcgtacgc
    20101 gacgagatca tagataaaat aacgaattac tttttaaata ttgcatagga ggatttccat
    20161 gacggagcat atcggacaaa aattgtcatt tctggatcgt tatctgaccg tatggatttt
    20221 tgcggctatg attcttggtg tggggctggg ctattttatt cccggcacgg aggcctttat
    20281 caattcattt caagtcggga ccaccaatat tcccattgcg gtgggcctga ttctgatgat
    20341 gtatccgccg tttgccaaag tgaaatacga ggaaatgcct caagttttcc gcaatggaaa
    20401 ggtcctaggc ctgtcgttgg tccaaaactg gctgatcggg cctgtcctga tgttttttct
    20461 ggcgattgtg ttcttgccgg ataaacccga ctacatggtg ggcctcataa tgattgggct
    20521 cgcgcgctgt atcgccatgg tcatcgtctg gaacgaactg gccaagggca atacagagta
    20581 tgccgccggc ctggttgcct tcaacagcat ttttcaggtg cttttctaca gtgtgtttgc
    20641 ctggtttttc attacgatcc tgccgcctta ctttggattt tcgggcagtg tcgtcgatgt
    20701 cagcatccgt caaatcgccg aaagcgtttt catctatctg ggcattccct gcatcgcggg
    20761 tgcgctgacg cgtctggccg gcgtaaaggt tatgggaaaa gaaaagtatc acacgcgctt
    20821 cgtgcctatc atcagtccgc ttaccctcat tgcgttgttg ttcacgattg ttgtcatgtt
    20881 cagtctcaag ggaaatctga ttatccaact cccgctggat gtcgtgcgca tcgccatacc
    20941 gcttttaatc tattttgtgg tcatgttcct tgtgtctttt tacatgggga ataagctagg
    21001 tgccgattat tccaaaacca cgacactggc tttcacggcg gccagcaata attttgaact
    21061 ggccatcgcc gtggccatcg ccgtcttcgg catcaactcc ggcgcggcgt tcgccgcagt
    21121 gatcggcccg ctggtggaag tgccggtgat gatcggcctg gtcatggtgg cgctgagatt
    21181 tcaggagaag tattttcaaa agtagtttga ggtgaatgct tcaatgttga aacttaaaag
    21241 attcccattg ctgctcgtga gtttgatggg gcatttgacc attttgctga cgttttccgg
    21301 ttccgtgacc cctgcttacg ggcagactgt tggacagggt attccgtcct ggggacaggg
    21361 gccttatgaa gtgattatat ttgccgatta tttctgtcct ccctgcaaac ggattgatgc
    21421 caaagccgaa ggcttgttca aagagctggt ggcgtcggaa aaagttaagt tgacgtttgt
    21481 cgatgttcca tttaaccgcg ccacgccgac ttacgcgaaa tattacctgt atgcggtgaa
    21541 agcggatgcc ggcataaaca acgcacttca tgtccgaaat gttctttttg aggcggctca
    21601 gggccgccgc gttcaggatg aaaacgcgct ggtcgctttt cttaaagaaa agaatactgc
    21661 gtggaaagcc atgaatgaaa agtccgtctt tccgcattta agcgccgtca tcaaagagca
    21721 caaggttgat gcgacaccca cttgcgttat taaatattct gccaaagata taaagaaata
    21781 tgttggtgac gatgaaattt gggatggact gacaaaattc aaagcgcatc tttggactgt
    21841 gaaataataa cgtaaaccag gttattttgt tcagaggaat tcgtcatgag aacaaaaggc
    21901 attttgctga tcgtcctttc acttgttatg ccgttttcct tatcagccgc cgatggcgta
    21961 acctttgaaa attacctcaa acgtttcgac tatcgggaac gcagggagat gaaaaccggc
    22021 atcgttgaaa tgctgaaact atacaatcag ggcaaagtac agatcattga tgttcgtttc
    22081 ccggaagaat acaaggctta cagtttcggt tttatcaaaa acattccgct aaacgaatta
    22141 ccgggcagac tcgacgaact ggataaggcg aagatcgttg tcacggcctg tcctcactac
    22201 gaccgtgccg aacttgcccg cacgtacctg aaacttcaag ggtatcaatc aaaatatctg
    22261 accgacggcc ttcttgggct tgccgaatat ctgcgcggcg acaaggcaaa agattttatg
    22321 tttgaaatga aaagataaaa gacccatgtc gattaggaga ggcaatgtga caaaagaaaa
    22381 aattatcatt tacgggacag acatgtgacc cttttgcatt caggctcgtg aagcctatgg
    22441 cgacaaggct gtctatatcg atgtcagcga agacccgaaa aaactcgaag aaatgctggc
    22501 ctattcaggc ggcaagatgc aggtgcccgt catagtggac ggcgataaaa tagcggtcgg
    22561 ttttgccggg gagatatccc tgcggggagg tattccgctt ttcggcggta cctgagcggt
    22621 aaaatgaagc ccttcggtgg ggggcaagga aaaaaagatt ggttatttga aaccgaattc
    22681 aacgatgagc ggaaaagaag aaagatagaa aacatgtccg aaaacgaagt gacgcaaata
    22741 cgtgtcaaag gcaatctggt cggcatcgtc ggcttgaaaa gtgtgattgc ggctatggcg
    22801 cctgacttca gccgcaactc cgatgaagcc atcggcgcgg aaatgatcaa aaggcttggg
    22861 ggtaaaaatt acattcccgc aagcgccaaa cccctttatg ccagggcttt ggtccgcgag
    22921 ttccgaatgt ttcttgggca accggtggaa gaggaagaaa ccggcggcgt cctcagagtc
    22981 atcattctgg ggccgggctg cgccaattgc agtagcctgg aaaacgatgt gcgcaatgtt
    23041 ctgtctgaaa tgaacgcgcc gggagaaatg gttcatgtta ccgatccacg tgagattgcc
    23101 aaatacgggg tgatgggtgt gcccgcgctg gtgattaatc agaaagtggc ttcagtggga
    23161 acgatgccgg aaaagaaaaa aatacggcag tggctggaag aagccctgaa gcaaatgtag
    23221 cgccgtatat cttgaaaaag aaggtcaacg gcacagttta aggaaatgag gaggatctgt
    23281 cttgtgaaag aatggcaaaa attattatgg attatcggca tctttcttgc cgctttttac
    23341 atcccgtggg aatcggtact gattcgtcag gcggggctgg aagcctttat gatgcttcag
    23401 gactatgcgc gccagcatgt gcttacctgc ctgatccccg cttttttcat tgccggagca
    23461 atcagcgtgt tcgtgtcgca ggcggccgtg ctcaaatatt tcggcgcgaa agccaataaa
    23521 atcctgtctt attccgtagc ctccgtttcc ggctccgtgc tggcggtttg ttcctgcacg
    23581 gtcctgccgc tttttgccgg catttacacg cgcggtgcgg ggatcggccc cgcgactgcg
    23641 tttttatatt ccggacccgc gatcaatgtt ctggcgatca ttctcacggc gaaggtgctg
    23701 ggctggcaat tggggcttgc acgcgcgatc ggcgcggtga ttttttccat tgtgacgggg
    23761 cttctgatgg cgtttatttt ccgcaaagac gacgccaacc gggagacggg cgagatcttt
    23821 ctgccggaag acgaaggcaa gggcagggcg ctgtggcagg atgccgttta tatgctggtc
    23881 atggtgctga ttctggtttt tgcttcgttt gccaagccat cggaggatga cggaagcgcc
    23941 tggcgcatga ttttcagcgt caagtggttt ttggtgatgg ggctgctggt cgttttgggt
    24001 atcatgctca aaaagtggtt tgtgaaagat gaactcaagg gctgggtgca ggctacctgg
    24061 gattttgcca agcagattct tccgcttttg ttcggcggcg ttttggtggc gggttttttg
    24121 ctgggccgtc ccggttatcc ggcactgatt cccgaatcgt atattcagat gctggtcggc
    24181 ggcaattcac tgggggctaa tttctttgcc tccgtagtcg gggcgttcat gtactttgcc
    24241 acactcaccg aagtgcatga acgccccgac tacggagg
//
LOCUS       DAOSIM010000005        24097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_2332_length_24097_cov_7.800932, whole genome shotgun sequence.
ACCESSION   DAOSIM010000005 DAOSIM010000000
VERSION     DAOSIM010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 24097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24097
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2332_length_24097_cov_7.800932"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PLR47_00465"
     CDS             complement(<1..411)
                     /locus_tag="PLR47_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG11432.1"
                     /translation="MLTLADHILDIAENSVRAGAKLIQIFIEEDSINDLLTLEITDDG
                     HGMAKDVLQKALDPFYTTKTVRRVGLGLPLLRDAAERSGGVFQVNSEENCGTTVKATF
                     GLRHLDRQPLGAIINIIIILIISNSDIDFFYKHRH"
     gene            complement(405..1151)
                     /locus_tag="PLR47_00470"
     CDS             complement(405..1151)
                     /locus_tag="PLR47_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOG11433.1"
                     /translation="MLKIYNCDLHIHTCLSPCAELDMHPKALVERALARHLDIIAVSD
                     HNASANVPYVMAAAAGKNIRIIPAMEITTSEEVHLLALFGSLSDLLELQSIVDRHLFG
                     ENDEDRFGVQAVVNERGEVEGLNNQLLIGATDLPIDAIVDIIHGLGGLAVPAHIDRES
                     FSVLSQLGFIDESSHFDALEISKRMGMREGRIRYAELARYPFVTSSDAHFLKDIGTST
                     TPILLKEATFEEIRMAVFGQNGRQILEKQC"
     gene            complement(1322..1663)
                     /locus_tag="PLR47_00475"
     CDS             complement(1322..1663)
                     /locus_tag="PLR47_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11434.1"
                     /translation="MNIEAIGAALGLEVKCGRESLGKNVTGGYTGDLLSDVMGNAKEG
                     YIWITRQVHQNIVAVASLKELAGIILINACEPARETLEKAEAEKIPILVSDLPAFELS
                     GRIYNLLTSGS"
     gene            complement(1680..2123)
                     /locus_tag="PLR47_00480"
     CDS             complement(1680..2123)
                     /locus_tag="PLR47_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG11435.1"
                     /translation="MGETKANLYSESFQIEGGNFNSAGIVSTSIKSILKKLGIPGDIV
                     RKAAIVSYEAEINVVSYAKKAVVNLTVKPGAVLIDVIDEGQGIPNIDLAMQEGYSTAD
                     HRIREMGFGAGMGLCNIKNYSDDFHIASEVGKGTHLKMTIQTPST"
     gene            complement(2110..2484)
                     /locus_tag="PLR47_00485"
     CDS             complement(2110..2484)
                     /locus_tag="PLR47_00485"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOG11436.1"
                     /translation="MKLSEVRDILDAEIIVGEDSLDLEVQTAFGADLMSDVLAFAKAG
                     SLLLTGLTNTQVIRTANVLDIAAIILVRGKKPSSETITLASELKIPILATKYILFETA
                     GRLYMKGIVGCLPKVETTSGRN"
     gene            complement(2600..3655)
                     /locus_tag="PLR47_00490"
     CDS             complement(2600..3655)
                     /locus_tag="PLR47_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOG11437.1"
                     /translation="MKKQRKFCVYCGGPVAKKKEDSVWRDFCSECRSFFYDNPLPVVS
                     AIVDVSREILLVKRDRAPFQGRWCLPTGFAEAGENIEEAVLRELGEETGIRGRISRLL
                     DVDSYKSRFYGDLLFLTFVVEKTAGKVAAGDDSAQAKFFPIDRIPPLAFRSNRRAVTA
                     YIQSKKDYWAILDAVDKRSNPSLRNAKRANSLTRRLIGNLLKNREAIIRQWIEDVSAN
                     PSTAEYRRYEPPVLHGICDRIISQITLWMGDAYGAPHIRNFYIKLGRERKKAGTLLSS
                     ALSALSLIRKHIWDVALARGALQKNLGLYATFELQRRMTIFFDLAAFYIARGYESSKT
                     EPRIPGAASSKRKRRAI"
     gene            complement(3652..4512)
                     /locus_tag="PLR47_00495"
     CDS             complement(3652..4512)
                     /locus_tag="PLR47_00495"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG11438.1"
                     /translation="MADPSDDKKIREVFRDVLNHREMERIIRRCSTNPDDIRQTALAG
                     ADLTSCRNVLELGCAFGSFTGALKCRLHPRARIAGLDIIPEYEPFFLEACAKAGYAGR
                     FSSDGVHQIAGFPPSDADLILCSFALYFFLDVLPDIARILKPDGLFITITHASGNMRE
                     LIDVIRSILKARRIIQPDDVLPIEEILKAFSAENGKALLAPHFGKIRQIDFPNTLVFP
                     PQDIGAFIDYYRFKRPFFLAGTPPAADIILPDLTRALAGMAAGRQTLSMCKDDAIFIC
                     SHPRSSKEAP"
     gene            complement(4574..5542)
                     /locus_tag="PLR47_00500"
     CDS             complement(4574..5542)
                     /locus_tag="PLR47_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG11439.1"
                     /translation="MMRVYFWGTRGSLPASITAGAVEEKIVKAIRASRTHSLETDEAI
                     EAFVHNELPFPVRASYGGNTACVELCGAEEYVLCDAGTGLRDFGGAYMKAKAAGQPGL
                     PNVFHLLMSHPHWDHLQGFPFFTPAYLPGHRIHIYGGHADLERIFIRQQSEPCFPVGF
                     HAMKADIRFHTLEPESEYDIAGMKVACIRQNHPGDSYGYRFEKDGRTVVYSTDSEHKD
                     GGREEYERFLSFFSNADLLIFDAQYRFLDAVDTKENWGHSSNLLGVEMAAAAGVRRLC
                     LFHNEHTFDDDALDRFLEDTRAYLKILDGSSSLKIDLAYDGLDIEL"
     gene            complement(5644..7332)
                     /locus_tag="PLR47_00505"
     CDS             complement(5644..7332)
                     /locus_tag="PLR47_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG11440.1"
                     /translation="MKKKILMVDDEPGSLFLLEALLNANGLETAMAENGKEALAMARL
                     NPPDLIVSDILMPVMDGYTLCREWKLDETLRSIPFVFYTAEYTRSRERALALSLDPDD
                     FILKPQEPHILIRLLIELLEKRKPAKPKIPGPAKEEIEFLRKHDKILFGKLEKKMADL
                     EKANEELREQEEKYRLSFEQVSDVIFILGTDLRILSISPSVERNLGYHPLEFVGKPFA
                     KIKKVMDPGCYAQASAKLASILEGKTVEPSDYIIFAKDGTLRHIETSATPVVKNGKTI
                     CIVCVTHDITQRKRAEESLINERNFSRTVAESLPGLFFVLDERGKFLRWNDNLRAVTG
                     YADEEIREMLFADLCPQSHRNAALEGVKEILRAGELSSEAELIFKDGTRRIFRLNARR
                     LSDEDRLCIVGAGMDVTDRKKAENELKRFAESLEEANIALRVLMHQRDEDQKDIEEKL
                     QANVHDLILPCLKKLKGANLEDRYKNDLQTLEYNLREILSPFLKNILAAHADLTPREI
                     QIMDLIKQGKKTGEIAAALNASLKTVETHRNHIRKKLNLVNAGVNLRSYLLSVS"
     gene            7680..8582
                     /locus_tag="PLR47_00510"
     CDS             7680..8582
                     /locus_tag="PLR47_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11441.1"
                     /translation="MDDCRVQLANLVDTRDPKAVLEEIHVIAGLMVPRFDFAPFDRAY
                     SDVVRLFRGEYPSYRSCNVQYHDLGHTLSVTLAVARLMHGAVEAGQSLTEKDLNMGLI
                     SGLMHDTGYIQEIADRQGTGAKYTQVHISRSISFLQAYYRGNPAFQNDLDSFRDILLC
                     TGLNTRVDAIRFAGATVELLGKILGTADLLGQMADRYYLEKLPELYEEFAEAGITTFS
                     SRLDLLDKTQGFYRMTLKRFEDELGNVQRFSRCHFRKRWGIDEDLYIQSIEKNMAYLA
                     RVVSLHRENYADHLRRFAHTKEHL"
     gene            8601..9416
                     /locus_tag="PLR47_00515"
     CDS             8601..9416
                     /locus_tag="PLR47_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOG11442.1"
                     /translation="MKRIVCLLTILLLAAGICGAAAAEDSLQNIKKRGVLRAGVKDLT
                     PSFALADIKTGEISGYDVDFVRAIARRLDVRLEITPFSGEENERISFLTEGKVDIVAA
                     TMTITREREKLIDFSHPYFVTAQKFLVHKDTVQRLSDLKNKQIGTVDQTTSVENLRKA
                     LPSASIVLFTGYVPAFLSLQNRQIFAVSTDEAILAGILAKSDKDVFEIPKVSISREPY
                     GLGVRKGQTSFLRFVNQALLEMEHSGEAKRIFDKWFGPESSAPLERTFRITAK"
     gene            complement(9461..9907)
                     /gene="rpiB"
                     /locus_tag="PLR47_00520"
     CDS             complement(9461..9907)
                     /gene="rpiB"
                     /locus_tag="PLR47_00520"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOG11443.1"
                     /translation="MKKIVIAGDHGAVALKDRIIAFLQRKNFEVTDLGTWGSESVDYP
                     DTAAAACREFLKGGYEFGILLCGTGIGVSIAANKIAGIRCALIGDPFSAEMARAHNNA
                     NFIAFGGRVRYAVPVEEMIEKFLSTPFEGGRHERRIAKIAALENSR"
     gene            10015..10902
                     /gene="xerD"
                     /locus_tag="PLR47_00525"
     CDS             10015..10902
                     /gene="xerD"
                     /locus_tag="PLR47_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOG11444.1"
                     /translation="MREWLNRYYQYLLIEKGAADNTLAAYGRDLNRYLAFLEQRGLSN
                     PWSALPQTVVDFLVQIKSEGLSANSANRTLAALRGFYKYLLREKVLEESPLAQIELAK
                     VWMRLPDTVSSDEMKKILSQPDDSTPQGLRDRAMLELLYASGLRVSELAGLTMNSINW
                     QVGFLTVMGKGGKERVVPVGKTAYDCVRRYVDEARPKLIRSKTTDVLFLNRFGTSLTR
                     QGLWKIIVHYAQKAGLKKNVHPHTFRHSFASHLLEGGADLRAVQVMLGHSDISTTQIY
                     THVTRERLKEIHRKYHPRG"
     gene            10907..12898
                     /locus_tag="PLR47_00530"
     CDS             10907..12898
                     /locus_tag="PLR47_00530"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG11445.1"
                     /translation="MRKPAGLKIGFGKKVFAAAGITALVLALLTVFFPKTFQTAGFYL
                     QRGINGFSYYILGASPHFYHLEMEKNASDFRVRAGETLDVTYRDEFVVKSVASDDWSG
                     KSLEPVLEGPGGKAGIIGVPFRGIDLVDKLLAGGVIPEGTQAAGVYQIAVYSTRDKIG
                     VVPLRVMITPQDWLRFAKSRGNPGEQEAYLKKAAAQNREDAGVRKILAGIYLQQNRIG
                     EAAKLYEEILRISPGDPLAMKELARCIAQLGREDRAIDLLSRLTEVQTEDAEAHAMLG
                     AAYGRKTIWDKAADSYFRAVQIDPDNHAVRLLLAQAYEKSRRTDAAVEQYRFVIRHAP
                     DAAPAWRAMGDMHLKSGRYAQAIESYTRVLDLQPKDAAAHANLATAYAGLGKAREEME
                     HLQKAAALSPDEPVIRFNLAAAYEKRGKTPDAVREYGRVLKNKPDDADALARLADLTF
                     QNKQYEQAAGYYEKLSRIHPTRASIFVRLGFARGELKQYAASAEDYEKALRLGAKDQA
                     LYYNLAFTYTKLGREKDAVRYYEKISPQNKKTLSILADHYLREKQYAQAIRYYQRILK
                     LEPKKASSWAGLGYAWLASGNPDKAIEHYSQALKYDREDDEIYANLGIAYEKKGLYPE
                     ALKAYRSAYEINPETRVAPRIPRLRIQLLHKKDNRGSEP"
     gene            12895..13728
                     /gene="kdsA"
                     /locus_tag="PLR47_00535"
     CDS             12895..13728
                     /gene="kdsA"
                     /locus_tag="PLR47_00535"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOG11446.1"
                     /translation="MKQVKLNSHTIGGKAGFILIAGPCVLENEASAMEIAVYLKELTD
                     RLEIPFVFKASYDKANRTSVAAYRGPGARAGLAILKNVREKIGVPVLSDVHRFEEIEE
                     AAEVLDVMQVPAFLCRQTDFVVEVAKKAKIVNVKKGQFLAPWDVANIIGKIQSAGNEN
                     ILITERGASFGYNNLVFDVRALPIMRAMGYPVIFDATHSVQLPGGMGTSSGGQRDMVP
                     YLARAAVAAGVDGIFMEVHPDPEKALCDGANSLYLNSLKELLTTLKKIDGITKGIGHG
                     S"
     gene            13885..14424
                     /locus_tag="PLR47_00540"
     CDS             13885..14424
                     /locus_tag="PLR47_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOG11447.1"
                     /translation="MKKKIRGKLEKIKMLILDVDGVMTDGRIIMDSDGREIKNFYVRD
                     GHGLVMLQRHGIRVAILTGRTSSVVDHRARELKIAQVYQGALNKKEVYAKILEKNGLA
                     PEETAYLGDDIVDIPVLKMAGFSAAVADAVDEVKKHVDYVTLCRGGKGAVREICEMLL
                     EALGFWPEVARRYDFDGPE"
     gene            14503..15015
                     /gene="lptC"
                     /locus_tag="PLR47_00545"
     CDS             14503..15015
                     /gene="lptC"
                     /locus_tag="PLR47_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOG11448.1"
                     /translation="MILVLAGAVLFALLRATGGKKETIVKILPDEADVRIADFFYTEV
                     GKDNLRWEVKAKTAQYQKKRNLALFDQVQMKLITQKGRTFVMTGDQGEMRTDSKDVEI
                     RGHVVATADTGEKFSTEVLRYSDARKQIYTDASVRMETSRMKIQGVGLSILMDKGELA
                     LSSGVKAKIY"
     gene            15029..15574
                     /gene="lptA"
                     /locus_tag="PLR47_00550"
     CDS             15029..15574
                     /gene="lptA"
                     /locus_tag="PLR47_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HOG11449.1"
                     /translation="MKKIVLITAGILVALNLQAWAQTGLLQKNTLRKNEPIQIVSDKM
                     EAFQATRMVVFSGNAVAQQGDVRIQADRLSIYYKNSAAQSEKVGPDAEQTGDLEKIVA
                     SGHVVITQKDVLATGNEAVYYQDTARFVMTGNPVLRQEKSVIKGCRVIIQVDENRGKV
                     EQCADENSGRVTAIIHPRKKK"
     gene            15604..16326
                     /gene="lptB"
                     /locus_tag="PLR47_00555"
     CDS             15604..16326
                     /gene="lptB"
                     /locus_tag="PLR47_00555"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOG11450.1"
                     /translation="MSHLSARGLVKIYNKRKVVNHIDLTISPGEVVGLLGPNGAGKTT
                     TFYMMVGLVKPDGGQIFLDDEEIGAYPMYRRARKGLNYLPQEPSIFRKLTVEENILAI
                     LETLQISEEERRRRLEELLGELDLTTLAKNRAYSLSGGERRRVEITRALVTSPKYILL
                     DEPFAGIDPLAVADIQNIIEKLKSKGIGIIISDHNVRETLACCDRAYIINEGAILVEG
                     RPDVIARSELAKKFYFGEDFSL"
     gene            16354..17826
                     /gene="rpoN"
                     /locus_tag="PLR47_00560"
     CDS             16354..17826
                     /gene="rpoN"
                     /locus_tag="PLR47_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOG11451.1"
                     /translation="MAFELRQNLKLSQQLIMTPQLQQAIKLLILSRQELVDTINQEME
                     ENPLLEEVAPDDESAEELHAEVAEDVQSVEKEESKIPEHTAELTGEGDGREEFDWNNY
                     LEDYGPVGVSYDRAEGDAPTLDNVVTESPSLTEHLMWQMKLSPFNGEEVRVGEQIIGN
                     LDSNGYLRATVEEIARLENVAEEFVEKVLKKVQEFDPAGVAARDLQECLLIQAGMFAR
                     ENPLIEPIIREHLKELELKNYLQIARRLKVPLKEVETAVLLISKMDPKPGAVYSEEKA
                     QSIIPDVYVVKSGDDYKIILNDDGLPRLRISNFYREIMGGVGGPDSAEKENGRKYIRE
                     KVQSATWLIKSIQQRQKTIYRVAESIVKHQKEFFDKGIDYLKPLVLRNIADDVEMHES
                     TISRVVTNKYMHSHRGIFEMKYFFGSSIQRTSGENIASKSVKEEIRQIIGKEDQKKPY
                     SDCEIVRLLKARNIDIARRTVAKYREMTGILPSSKRKKYF"
     gene            17983..18513
                     /gene="raiA"
                     /locus_tag="PLR47_00565"
     CDS             17983..18513
                     /gene="raiA"
                     /locus_tag="PLR47_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOG11452.1"
                     /translation="MNISVTFRTGEGENWQKEYAEEKIARLKKYLDVPADVHMILSTE
                     KFRNMVEINLTANGWNVNAKEEAKDMRLAVDRCIVKIENQLKKQKEKSRQHKPKSIRH
                     AGDKALEGAEEEDAASKVVETRKIILKPMSLEEAVLELESGKARFLIYRDTASEKVSL
                     VHRLDDGQLAHIETNS"
     gene            18619..19086
                     /locus_tag="PLR47_00570"
     CDS             18619..19086
                     /locus_tag="PLR47_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOG11453.1"
                     /translation="MRLDQIFKMEFLSENLLSDTKEGVLAELISLLIGSGLKIDRAKA
                     LDVLQQREKLGSTGIGDGVAIPHGKVSELQDMVIAFGRSRRGVAFDAIDGKPVQLFFL
                     LLAPEQSTGQHLKALARISKMLKTPNFRKTLVDAKNRADLYKAIVEQDATCPL"
     gene            19094..19948
                     /locus_tag="PLR47_00575"
     CDS             19094..19948
                     /locus_tag="PLR47_00575"
                     /inference="COORDINATES: protein motif:HMM:NF019118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11454.1"
                     /translation="MPHPEASAREWFRDYLPYLGIRTISGPRNHKNPFPAGAVQCLRS
                     PTAFPRREAAILLFPPETANKLLALSGPSREEYFSRMAGRRPVLLIFSQSPSLPAVIR
                     NQLRNHCLWAAASPLHENLLASRIRSILLEKIHQCMAMHGVALACGDRGVLLTGPSGI
                     GKTTAALETAREGCFWIADDLAVIKNVRGRLMISGHPRIQKYVHAGEKGIEFANRVLP
                     ARRTKQRAELFGIVDVCRSAGKKISRPIEIRILEKRLPLVRVCISRTGYLRQNLLTRA
                     MQKLNEVG"
     assembly_gap    19985..19994
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19995..20829
                     /gene="rapZ"
                     /locus_tag="PLR47_00580"
     CDS             <19995..20829
                     /gene="rapZ"
                     /locus_tag="PLR47_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOG11455.1"
                     /translation="LSGSGKSTALRALEDIGFFCVDNLPVVLLPKFLSITLQSSPKIR
                     QVAMVMDLRQKSFLEKYQRIFERLKEKGYRINILFLEAGEESLLRRFSETRRSHPLSE
                     KRSIMEGIQEEVKKLSTLRQMADTVIDTTTSNVHQLKDTVQKQFLPSSTRKKMMISVM
                     SFGYRYGLPADADLAIDVRFLPNPYYVEKLKNLNGHHRDVEKYVLNNEECKTFMNKAA
                     DMLNFIIPLYEKEGKVRLNVAFGCTGGKHRSVVMANQFSLFFQKQKFIVHTTHRDINK
                     S"
     gene            complement(20974..22296)
                     /locus_tag="PLR47_00585"
     CDS             complement(20974..22296)
                     /locus_tag="PLR47_00585"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG11456.1"
                     /translation="MKKTGIIAAAVVIVLAAGALGWLYFAGWLDRDKPAIRLDSDITA
                     IGKKKDISIAFSDERSGLSGLKVEIVQDNKPRLLAQESFPSRSTRQKVLRLTVEPLLL
                     QLNNGPAVVRITAGDASLFKNETVISEQVRIDMMPPQVYVMSPVNYINQGGAGFIPYR
                     TSKPAALTGVFVDDRFYPGYNIVIGGQPTSVATFAVGTGASASTKIVIFARDSAGNEA
                     RTSMPFTIKPKKFRADKVNLSETFLQKIMPEFQGSIPQLQGKTPVEVFGYVNTTLREE
                     NSKTIQSVCRKSVNKKLWEGAFHRMRNASPMALFGDERTYLVDGKPFGNSVHLGVDLA
                     STAHAPIEAANNGIVVFAGALGIYGNAVIIDHGLGLFSLYGHLSSIETAVGKPVKKEE
                     KIGLSGLSGLAGGDHLHFSILVGGQFVNPQEWWDPHWIEDNVMKKMNL"
     gene            22471..23685
                     /gene="coaBC"
                     /locus_tag="PLR47_00590"
     CDS             22471..23685
                     /gene="coaBC"
                     /locus_tag="PLR47_00590"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOG11457.1"
                     /translation="MLKNKKIVLGITGGIAAYKSAELTRAFIKAGAEVKVIMTQSATE
                     FIAPLTLRTLSRNPVYADMFEDAEPYDMAHIALAEWADAFVVAPATGNVIGKIASGIA
                     DDLLTTTLMAENRPTLICPAMNDKMLANAFVQENLRKLAGAGYRVMQSGVGELACNAA
                     GAGRLPEIPEILDEMEALLTVQDFAGEKVLVTAGPTQEPLDPVRYITNHSSGKMGYAL
                     AKAAHQRGAQVTLISGPTRIVPPKVETVVFVRTAQEMHRAVMDAWTKASVIIKAAAVA
                     DYRPRVAAGEKIKKDRGLSGIELVRNPDIIADIGAKKKKGVLVGFAMETEDLLDNARR
                     KMIRKNMDMIVANNLRDDGAGFEGDTNIITILDRLGHTVALDKMTKQDAAHRILDHIR
                     SFKSKKGPAGKK"
     gene            23682..>24097
                     /locus_tag="PLR47_00595"
     CDS             23682..>24097
                     /locus_tag="PLR47_00595"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOG11458.1"
                     /translation="MTQSFPPSSSDEIRNELLSVVKSVRGQVLDAERLYVALDCKRPQ
                     PARKNTPDGSDRGEALHSLSLEAANCRQCPLAQTRKNVVFGAGSLSAQVVFVGEAPGA
                     DEDEQGQPFVGKAGQLLTDIIVKGMKLTRKDVYICN"
BASE COUNT         5468 a         6405 c         7128 g         5086 t
ORIGIN      
        1 atgcctgtgt ttataaaaaa aatcaatatc agaattagaa ataatgagaa taattattat
       61 gttaattata gcacccagag gctgccggtc caaatgacgc aggccaaacg tggcctttac
      121 cgtagtgccg caattttctt cagaatttac ttgaaagaca ccgccggagc gctccgcggc
      181 gtctctcagc aggggcagac ccagacccac gcgacggacc gttttggtcg tataaaacgg
      241 atccagcgct ttttgcagga cgtccttggc catgccgtgc ccgtcgtcgg tgatttcaag
      301 cgtcagcaga tcgtttatgg aatcttcctc aatgaagatt tgaatcagct tcgcgcccgc
      361 gcgcacggag ttttcggcga tgtccaggat atgatcggcc agcgtcagca ttgcttctcc
      421 agaatttggc ggccgttttg tccgaagacg gccattctga tttcttcaaa ggtcgcctct
      481 ttcagaagga tgggggtggt ggatgttccg atgtccttta gaaaatgggc gtcggaagac
      541 gttacaaagg ggtaccgggc aagctcggca taccggatgc gcccttcacg catgcccatg
      601 cgtttggaga tttccagcgc gtcaaaatgc gagctttcgt cgatgaagcc gagctggctc
      661 agcacgctga agctttcccg gtcgatgtgg gcggggacgg cgagtccccc cagcccgtga
      721 atgatgtcca cgatcgcatc gatgggaaga tccgtagcgc cgatgaggag ttggttgttg
      781 agcccctcca cttccccccg ctcgttgacg acggcctgca cgccgaagcg gtcttcgtca
      841 ttttctccaa acaggtgccg atcgacgatc gattgaagct ccagaagatc ggacaggctc
      901 ccgaagagcg ccagcaggtg cacttcctcg ctggtcgtga tttccatggc cggaataatg
      961 cgaatgtttt ttcccgcggc ggccgccatg acatacggga cattggcgga agcgttgtgg
     1021 tcgctgacgg cgatgatgtc gagatgccgc gcgagtgccc gctccaccag ggccttcgga
     1081 tgcatgtcca gctcggcgca gggcgacagg caggtgtgaa tgtgaagatc gcagttataa
     1141 attttcagca tggcttcttg catcgctcct ggcggaagcg cccctgaggg ggatcgcctg
     1201 aaggtcgcgg ggcagagggg ggaagggggg ctgggttttc gcttcggccc ctttcccggg
     1261 cggggaaaag aaggcgcctt gcaggaatca ttcgccgcga acggccgggc ggcttcggcg
     1321 atcagcttcc ggatgtcagc agattgtaaa tccggcccga gagctcaaag gccggcaggt
     1381 cggacaccag gatgggaatc ttttccgcct cggctttttc cagtgtttcc ctggccggtt
     1441 cgcaggcgtt gatcaggatg atgcccgcga gctccttgag gctggcgacg gcgacaatgt
     1501 tctggtgcac ctggcgggtg atccagatat agccctcttt ggcgttgccc atgacatcgc
     1561 ttaagaggtc tccggtgtag ccgccggtga cgtttttgcc caggctctcc cggccgcact
     1621 taacctccag ccccaaagcc gcgccgattg cttcaatgtt catacactaa ccctttattt
     1681 caagttgatg gcgtttgaat ggtcattttc aaatgcgtac ccttgccgac ctccgaggcg
     1741 atgtggaagt cgtcggagta atttttgatg ttgcaaagcc ccatacccgc gccgaagccc
     1801 atttcccgga ttctgtgatc ggcggtggag tatccttcct gcatcgccag atcgatattg
     1861 ggaatgccct ggccttcgtc gatcacatcg atgagcaccg cgccgggctt gaccgtcagg
     1921 tttacgacgg cttttttggc gtaggataca acattgattt ccgcctcgta cgagacgatg
     1981 gcggctttcc ggacgatgtc tccgggaatg cccagttttt tcagaatgga tttgatgctg
     2041 gtcgacacaa tgccggcgga attgaaattg ccgccttcaa tctgaaaact ttccgaatag
     2101 aggtttgctt tagtttcgcc cactggtggt ttcaactttt ggcaggcagc cgacgattcc
     2161 tttcatgtag agacgccccg ccgtttcaaa gaggatgtat tttgtggcca gaatggggat
     2221 tttcagttcg ctggccaaag tgatcgtttc ggaagacggc tttttccccc gcaccagaat
     2281 aatggcggcg atgtccagca cgttggcggt ccggatcacc tgggtgttgg tcaggccggt
     2341 caggagcagg ctgcccgcct tggcgaaggc caaaacgtcg ctcatcaggt cggcgccgaa
     2401 ggcggtttgt acttccaggt caaggctgtc ttcgccgaca atgatttccg cgtccagaat
     2461 atctctcact tcactcagtt tcaaaggctg ctcctcgtca atccgcaaaa tggtttacag
     2521 ggatccaaca gttcccgccg ctttcgtttc cggaaaaccg cagtcttttt atcatggctt
     2581 atgaccttcg tcaataagat taaatggcgc ggcgctttcg ttttgaagag gcggcgccgg
     2641 ggattcgggg ctctgttttc gatgactcgt aaccccgtgc gatgtaaaaa gcggccaggt
     2701 cgaaaaaaat ggtcatgcga cgctgcagct cgaaggtcgc gtaaaggccg aggttcttct
     2761 gcagcgctcc ccgggccagc gcgacgtccc agatatgctt ccggatcagg ctcagcgcgc
     2821 tgagcgcgct gctgaggagc gttccggcct tttttctctc gcggcccagc ttgatgtaga
     2881 aattccggat gtgcggcgct ccgtaggcgt cgcccatcca gagcgtgatc tgcgaaataa
     2941 tccggtcgca gatgccgtgg agcaccgggg gttcgtagcg gcggtattct gcggtggacg
     3001 ggttggcgga cacgtcttcg atccattgcc gaatgatggc ctcccggttt ttcaaaaggt
     3061 tgccgatcag acgccgggtc agggaattgg cgcgcttcgc gttgcgcagc gatggattgg
     3121 agcgcttgtc gacggcgtcc aggatggccc agtagtcttt tttgctttga atgtaagccg
     3181 tgacggcgcg gcggttggag cggaacgcga gcggcgggat gcggtcgatc ggaaaaaact
     3241 tcgcctgcgc gctgtcgtcg cccgccgcga ctttcccggc ggtcttttcc acgacaaatg
     3301 tcagaaagag caggtcgccg taaaaccggc ttttgtagga atcaacgtcc agaagcctgc
     3361 tgatccggcc ccgaatgccg gtttcctcgc ccagttcgcg caggacggcc tcttcgatgt
     3421 tttctccggc ttccgcgaat ccggtcggga ggcaccagcg gccctgaaac ggcgcccggt
     3481 cgcgcttcac cagcaggatt tccctcgaga catccacgat cgccgagacc accggcagcg
     3541 gattgtcgta aaagaacgag cggcattcgg aacagaaatc ccgccaaacc gaatcttctt
     3601 ttttcttcgc gaccgggccg ccgcagtaca cgcagaattt ccgttgtttc ttcatggagc
     3661 ttcctttgat gaacggggat gtgaacagat gaaaatcgcg tcgtccttgc acatggacag
     3721 cgtttgacgg ccggccgcca tgccggcaag cgcccgggtc agatcgggga ggatgatgtc
     3781 tgccgccggc ggcgttcccg ccagaaaaaa cgggcgcttg aagcggtagt aatcgatgaa
     3841 cgccccgatg tcctgcgggg gaaaaaccag cgtattgggg aaatcgattt gccgaatctt
     3901 cccgaaatga ggggccagaa gcgccttgcc gttttccgcc gagaacgcct ttagaatttc
     3961 ttcaatgggc aggacatcgt ccggctggat gatccggcgg gccttcagga tgctccgaat
     4021 gacgtcgatc agttcgcgca tgtttccgga cgcatgcgtg atcgtgatga ataggccgtc
     4081 cggcttcaga atacgggcaa tgtccggaag aacgtccaaa aaaaagtaaa gggcgaagct
     4141 gcagagaatc agatcggcgt ccgacggcgg aaagccggcg atttgatgaa cgccgtcgct
     4201 ggagaaccgc ccggcatagc cggcttttgc gcaggcctcc agaaaaaacg gctcatattc
     4261 ggggatgatg tcaagaccgg caatgcgggc ccgcggatga agacggcact tgagcgcgcc
     4321 ggtgaaggag ccgaacgcgc atcccagttc caaaacattc cggcacgagg tcagatccgc
     4381 cccggccagg gccgtctgcc gaatatcatc cgggttggtg gaacagcgcc ggataatccg
     4441 ttccatttcc cgatggttca gcacatcgcg aaaaacctca cggatttttt tgtcatcgga
     4501 aggatcggcc atgttttccc ctgcaatgcc ggcatgcggg gcgggtattt gccgcctggt
     4561 tgcgcgcgcc cggctagagt tcgatgtcca ggccgtcata ggccaggtcg attttaagac
     4621 tggacgaacc gtcgaggatt ttgagatagg cgcgggtatc ctccagaaac cggtccagcg
     4681 cgtcgtcgtc aaaggtgtgc tcgttgtgaa agaggcacag gcgccggaca ccggcggccg
     4741 ccgccatttc gacgcccagc agattgctgg aatggcccca gttctccttg gtgtccacgg
     4801 cgtccaggaa acggtattgc gcatcgaaga tcagcaggtc tgcatttgaa aaaaaagaga
     4861 ggaaccgctc gtactcctcc ctgccgccgt ccttgtgctc cgagtcggtg gagtagacga
     4921 cggtccggcc gtctttttca aagcggtacc cgtaggagtc tcccggatgg ttctgcctga
     4981 tgcaggcgac cttcattccg gcaatgtcgt attcgctttc aggctcaagg gtgtggaagc
     5041 ggatgtcggc cttcatggca tgaaaaccga cggggaaaca gggttcgctc tgctggcgga
     5101 taaagattcg ttcaagatcc gcatggcccc cgtagatatg gatgcggtgg cccggcaggt
     5161 aggcgggcgt gaaaaaggga aagccctgaa ggtggtccca gtgaggatgc gacatcagca
     5221 ggtggaaaac gttcggcaaa ccgggctgcc cggcggcttt cgccttcatg taggcgccgc
     5281 cgaaatcccg aagccccgtc ccggcgtcgc aaaggacata ttcttccgcg ccgcaaagtt
     5341 cgacgcatgc cgtgtttccg ccgtagcttg cccggacggg gaaggggagt tcgttgtgaa
     5401 cgaaggcttc gatggcctcg tccgtctcca gagaatgggt gcgggaggcc cggattgcct
     5461 ttacgatttt ctcctcgacg gccccggcgg tgatcgaggc gggcaaagat cctcttgtcc
     5521 cccagaaata tacccgcatc atccttcctc ctcatggtgt ccggtatttc ttccatggtc
     5581 cgcggtgaag gcattttgtc atattgccgc gggcatcgca acgaaaatta tccgtatcgt
     5641 ccgttacgac acggacagca ggtatgaccg aaggttaacc ccggcattga ccaggttgag
     5701 ctttttccgg atatgattcc gatgggtttc caccgttttt aacgaagcgt tcagcgcggc
     5761 ggcaatttcg ccggtttttt tcccttgttt aatcaggtcc ataatctgaa tttccctggg
     5821 cgtcaggtcc gcgtgagcgg caagaatgtt tttcaaaaaa ggggacaaaa tctcccggag
     5881 attgtattcc agcgtctgga gatcgttttt gtagcgatcc tcgagatttg cgcccttgag
     5941 ttttttcaag cacggaagga tgaggtcatg gacgttggcc tgcaacttct cttcgatgtc
     6001 tttctggtct tcgtctcgct ggtgcattaa aacgcgcagc gcgatgttgg cttcctcaag
     6061 gctttcggca aagcgcttca gctcgttttc cgctttcttc cggtcggtca cgtccatgcc
     6121 ggcgcccacg atgcacaacc ggtcttcatc ggacaggcgc cttgcgttga gccggaaaat
     6181 tctacgggtc ccgtctttga aaatcagctc cgcctcggag gagagctccc cggcgcgcaa
     6241 aatttctttg acgccctcca gcgcggcgtt tctgtggctt tgaggacaaa ggtccgcaaa
     6301 cagcatttcc cggatttctt cgtccgcata tccggtcacg gccctcagat tgtcgttcca
     6361 gcggagaaat ttcccccgct cgtccagaac aaagaacagg cccggcaggc tttccgcgac
     6421 cgtccggctg aaattccgct cattgatcag agattcttcc gcccgcttgc gctgagtgat
     6481 gtcgtgcgtg acgcagacga tgcagatggt ctttccgttt ttcacaacag gagtcgcgct
     6541 ggtttcgatg tgccgcaggg ttccgtcctt ggcgaagatg atatagtccg acggctcaac
     6601 ggttttcccc tccagaatgg aagccagctt ggcgctggcc tgcgcgtagc agccgggatc
     6661 catcaccttc ttgattttcg caaacggttt gccgacaaac tccagcggat gatagcccag
     6721 atttctttcg accgagggag atatgctcag tatccggagg tccgtgccga gtatgaaaat
     6781 gacatccgac acctgttcaa agctcagccg gtatttctcc tcctgctccc ggagctcctc
     6841 gttggccttt tccaggtccg ccattttctt ttccagcttg ccgaatagaa ttttatcgtg
     6901 cttgcgaaga aattcaattt cttctttcgc cgggccggga atttttggtt tggccggttt
     6961 tcttttttcc agcagttcga tcagcagacg gatcagaata tgcggctcct ggggtttgag
     7021 aatgaagtcg tctggatcca aactcagggc cagcgcccgt tcccgggatc tcgtgtattc
     7081 ggcggtgtaa aatacaaaag ggatggaccg gagcgtttcg tcgagtttcc attcccggca
     7141 gagggtatag ccgtccataa ccggcatcag gatgtccgag acaatgagat ccggagggtt
     7201 gaggcgggcc atggccaggg cttctttgcc gttttccgcc atggccgttt ccaggccgtt
     7261 ggcgttgagc agggcttcca gcagaaacag gctgcccggt tcatcgtcca ccatcagtat
     7321 tttctttttc atggccgcct cgacgggaat tctttcgcgg atgatccaag gttatctaaa
     7381 gcttttttcg ggtggaggca agataaaaat ctgaacagat aaaatccgaa caagcgcccc
     7441 cgggcctgcc ggaaaggctt tttaacgctc cgaacacggg cgccgggctt gctttttggc
     7501 ttccgtcagc aatatccatt gatgaatttt cagaaatatg ccagattacc gcccatcatc
     7561 atggaatcag cggttcggcg ccgtaaaatc ggtcggcgta cggcggtccg gggcggcatg
     7621 acgcgggacc ggcggaaaag ggcttttcgg cgcgccgacg ccgctgacat ggagcgctga
     7681 tggacgattg tcgcgtgcag ctggccaatc ttgtggacac ccgggacccc aaagccgttc
     7741 ttgaggaaat tcatgtgatt gccggtctga tggtgccgcg gtttgatttt gcgcctttcg
     7801 accgggccta ttcggacgtg gtccgtctct ttcgcggcga atatccgtcc tatcgctcct
     7861 gcaacgtcca ataccatgat ctcggccata ccctttcggt cacacttgcc gtggcccgcc
     7921 tgatgcacgg agccgtggaa gccggacaat cgctaaccga aaaagacctc aacatgggtc
     7981 tcatcagcgg cctcatgcac gataccggct atattcagga aattgccgac cggcagggga
     8041 ccggggccaa gtacacgcag gttcacatca gccgcagcat ctcgtttctt caagcctatt
     8101 accgcggcaa tccggcgttc caaaacgatc tggattcgtt tcgggatatt ttgctgtgca
     8161 cggggctcaa cacccgtgtg gacgccatcc ggtttgccgg cgcgacggtt gagctgctgg
     8221 ggaaaatcct gggcacggcg gatctcctgg ggcagatggc cgatcgctac tatctggaaa
     8281 agctgccgga actctacgag gagttcgcgg aggccggcat cacgaccttt tccagccggc
     8341 tggatctttt ggataagacg caggggtttt accggatgac actgaagcgt tttgaagatg
     8401 aactgggaaa cgtgcagcgt ttttcccgct gccacttcag gaagcgctgg ggcattgatg
     8461 aagacctcta tattcagagc attgaaaaaa acatggcgta tctggcccgg gtggtcagtc
     8521 ttcaccgcga gaattacgcg gatcacctgc gcaggttcgc tcatacaaaa gaacatcttt
     8581 aatggtcatg gaggattaaa atgaaaagga ttgtatgtct gttgacgata ctgttgctcg
     8641 cggccgggat ttgcggggct gccgcggcgg aagactcact gcaaaacatc aaaaaaagag
     8701 gcgttttacg ggccggcgtc aaagacctga ccccgagctt cgcgctggcc gacatcaaga
     8761 cgggggaaat cagcgggtat gacgtggatt ttgtccgcgc catcgcccgc agacttgacg
     8821 tccgccttga gatcactccc ttcagcgggg aggaaaatga gagaatttcc ttcctgacgg
     8881 agggcaaagt cgatatcgtt gcggccacca tgaccatcac gagagaaaga gaaaagctga
     8941 tcgatttcag ccacccgtat tttgtgacgg cccagaaatt tctggtgcac aaagacaccg
     9001 tgcagcggct gagcgatctg aagaacaagc aaatcggaac cgtggatcag accacttcgg
     9061 tggagaatct ccgcaaagct ctcccctcgg cgtccatcgt gctcttcacc ggttatgttc
     9121 cggcgtttct gtcgctgcag aacaggcaga ttttcgccgt ttcgacggat gaggccatcc
     9181 tggccggcat tctggcaaaa tccgacaaag acgtatttga aattccgaag gtcagcattt
     9241 ccagagagcc ctatggcctg ggggtccgca aggggcagac ttcatttttg cgttttgtga
     9301 atcaggcgct tctggagatg gagcacagcg gcgaagccaa aagaatcttt gacaagtggt
     9361 tcgggccgga gagctccgcc ccgctggaga ggacgttcag gatcaccgcg aaataaagag
     9421 taaaaacgcc ccgcggcgca agaaagcggg ccggtgcgaa tcagcggctg ttctccagtg
     9481 cggcgatctt ggcgattctt cgttcgtgac ggccgccttc gaacggggtg ctcaggaatt
     9541 tttcgatcat ttcctcgacc ggcacggcgt agcgcactct gccgccgaag gcgatgaagt
     9601 tggcgttatt gtgagccctg gccatttcgg cgctgaacgg atcgccgata agggcgcagc
     9661 ggatgccggc gattttgttg gcggctatcg aaacgccgat gcccgtgccg caaagcagaa
     9721 tgccgaattc gtagccgcct ttcaggaatt cccggcaggc ggccgcggcc gtatccgggt
     9781 aatcgacgga ttcgctaccc catgttccaa gatcggtgac ctcgaaattc ttcctttgca
     9841 aaaaggcgat gatcctgtcc ttcagcgcaa ccgctccatg atcgccggcg atgacgattt
     9901 ttttcatctg cggactcccc ctcctgaccc ttgatgacgc ttctttctac aagatgccgg
     9961 ggaatgtcaa caaaaaatct ttacatcagc atcggatttg cgtagaaaaa tgccatgcgc
    10021 gaatggctga accggtatta tcaatatctg ctgatcgaga aaggggctgc ggacaacacg
    10081 cttgcggcgt acgggcgtga cttgaaccgc tatctggcct ttctcgaaca gagggggctt
    10141 tcgaatcctt ggtcggcact tccgcagacg gtggtggatt ttctggtgca gatcaagtca
    10201 gaagggcttt cggccaattc ggcaaaccgg acgctggccg ccctgcgcgg cttttacaag
    10261 tacctgctga gggaaaaggt tttggaggaa tcgccgctgg ctcaaattga gctggccaaa
    10321 gtctggatgc gtctccccga cacggtcagt tccgatgaaa tgaaaaagat cctgtcccag
    10381 ccggacgatt cgaccccgca gggtttgcgg gaccgggcga tgctggagct gctctacgcg
    10441 tccgggcttc gcgtgtcgga gctggccggc ctcaccatga acagcatcaa ctggcaggtg
    10501 ggttttctga ccgtcatggg gaagggcggc aaggagcggg tcgtgcccgt cggcaaaacg
    10561 gcctatgact gcgtgcggcg atacgtcgat gaagcgaggc cgaagcttat ccggtccaaa
    10621 acgacggacg ttttatttct gaatcgcttc ggaacgtccc tgacgcgcca gggactttgg
    10681 aaaatcatcg tccattacgc gcaaaaagcg ggcttgaaaa aaaatgtgca cccgcataca
    10741 ttccggcatt ccttcgcctc ccatctgctg gagggggggg cggatttgcg cgcggtgcag
    10801 gtgatgctcg gtcattcgga tatttcgacg acgcagattt acacgcatgt aacccgggaa
    10861 cgcctgaaag agattcaccg gaaatatcat ccaagaggtt gaagatttgc gaaaaccggc
    10921 agggcttaaa atcggattcg gcaaaaaagt ctttgcggcg gcgggcatca cggcgctggt
    10981 tttggccctt ttgacggtgt ttttccccaa aacctttcaa acggccggct tttatctcca
    11041 gagaggaatc aacggttttt cttattatat tctgggggcc tcgccgcatt tttatcattt
    11101 ggagatggaa aaaaacgcga gcgattttcg cgtcagagcc ggagaaaccc tggacgtaac
    11161 gtatcgcgat gaattcgtgg tcaaatccgt ggccagtgat gactggagcg gaaaaagcct
    11221 ggagccggtt ctggaggggc ccggcggcaa agccggcatc atcggcgtgc cgtttcgcgg
    11281 aattgacctg gtggacaaac tgctcgccgg cggcgtgatt cccgagggga cgcaggccgc
    11341 cggtgtttat caaattgcgg tctattccac gcgggacaaa atcggcgtgg tgcccctccg
    11401 ggtgatgatc acgccgcagg actggctgag gttcgcaaag agccgcggca atccgggcga
    11461 gcaggaggcg tacctgaaga aggcggctgc tcaaaaccgg gaagacgccg gcgtgcgcaa
    11521 gatccttgcc ggcatttatc ttcagcaaaa ccggatcggg gaggccgcga agctgtatga
    11581 agaaattttg cgcatcagcc ccggcgatcc cctcgcgatg aaagaactgg cccgctgcat
    11641 tgcacagctc ggccgggagg accgcgccat cgatcttttg tcccgtctga cggaagtcca
    11701 gacggaggat gccgaagccc atgccatgct cggagccgcc tacgggcgca aaaccatctg
    11761 ggacaaagcg gcggacagct atttccgggc cgtccagatc gatccggaca accatgcggt
    11821 gcggcttttg ctggcccaag cctatgagaa atcccgccgg accgacgcgg cggtggaaca
    11881 gtaccgcttc gtcatccgcc atgcacccga cgcagccccg gcctggcggg cgatgggcga
    11941 catgcacctg aagagcgggc gatacgcgca ggcgattgag agctacacgc gcgtcctcga
    12001 ccttcagccg aaagacgcgg cggcgcatgc caacctggcc acggcctacg ccggtctggg
    12061 caaagcgagg gaggaaatgg agcacctgca gaaggccgcg gcgctgtcgc ccgacgagcc
    12121 ggtgatccgc ttcaatctcg cggccgctta tgaaaaaagg ggcaagacgc ccgacgccgt
    12181 ccgggaatac ggccgggttt tgaaaaacaa gccggacgat gccgacgcgc tcgcgcgcct
    12241 ggcggatctg acctttcaaa acaaacagta tgagcaggcc gccggttatt acgaaaaact
    12301 ctcgcggatt cacccgacgc gcgcgtcgat attcgttagg ctgggatttg cccgcgggga
    12361 actgaagcag tatgccgcat cggccgaaga ttacgaaaag gctctccggc tgggggcaaa
    12421 agaccaggcc ctgtattaca atctggcgtt tacctatacg aagctgggca gggaaaagga
    12481 tgccgtccgc tactacgaaa aaatatcgcc ccagaataaa aaaacgctga gcattctggc
    12541 cgatcattac ctgagagaaa aacagtacgc ccaagccatc cgttattacc agagaattct
    12601 gaaactggag cccaaaaagg cgtcttcctg ggccggcctg gggtatgcct ggcttgcctc
    12661 cggaaacccg gataaagcga ttgagcatta ttcgcaggct ttgaagtacg accgcgagga
    12721 tgacgagatt tacgccaacc tgggcatcgc ctatgaaaaa aaagggcttt acccggaagc
    12781 cctgaaagct tataggagcg cctacgagat caatccggaa actcgggtgg cgccgcggat
    12841 tccgaggctg cgcattcaac ttttgcataa aaaggataac agaggaagcg aaccatgaag
    12901 caagtgaagc tgaattccca cacgatcggc ggcaaggccg ggtttatttt gatagctggc
    12961 ccctgcgttc tggaaaatga agcatcggcg atggagattg ccgtgtatct gaaagagctg
    13021 acggacaggc tggagattcc ctttgtcttt aaggcatcct acgacaaggc caaccggacg
    13081 tcggttgccg cttaccgcgg gccgggcgcc cgcgcggggc ttgccatatt gaaaaacgtc
    13141 cgggaaaaaa tcggcgtgcc ggtcctttcc gacgtgcacc ggtttgaaga aattgaggaa
    13201 gcggcggagg tgctggatgt gatgcaggtg cccgcgtttc tgtgccggca gacggatttt
    13261 gtcgtggagg tcgcgaaaaa agcgaagatc gtgaatgtga aaaaagggca gtttctcgcg
    13321 ccctgggacg tggccaacat catcggaaag attcagtcgg ccggcaatga aaatattctg
    13381 atcaccgaac ggggcgcgtc ctttggatac aacaatctgg tgttcgatgt ccgcgccctg
    13441 ccgatcatga gggcgatggg ataccccgtc atttttgatg ccacgcattc ggtgcagctg
    13501 ccgggcggca tgggaacctc gtccggcggc cagcgggaca tggtgcccta cctggcccgg
    13561 gcggccgtcg cggcgggcgt ggacggcatt ttcatggaag tccatccgga tcctgaaaaa
    13621 gcgctgtgcg acggggccaa ttcgctttat ctgaattcgc ttaaagaact gctgacgacg
    13681 ctgaagaaga tcgatggaat aacgaaggga ataggccatg ggtcataggc gatgggcgat
    13741 agggaaaaag ctcatagctc atagtgaaga gcgatgagtg gcgagtaaat tgttgggcgg
    13801 gtcttcagac ccgacgtgat gaacagtgga tggcggatgg taaaaaaaag caacgagcgg
    13861 aaagcgatga gtgaagagcg aataatgaaa aagaagatac ggggaaagct tgaaaagata
    13921 aagatgctga ttctggatgt ggacggggtg atgacggacg gcagaatcat catggacagc
    13981 gacggacggg agataaaaaa cttttatgtc cgcgacggcc acgggctggt catgcttcag
    14041 cgccacggca ttcgggtcgc gatcctgacc ggacggacat cttcggtggt cgatcatcgg
    14101 gcgcgcgagt tgaaaattgc gcaggtatat cagggggcgc tgaacaagaa ggaagtttat
    14161 gcaaaaattc ttgagaaaaa cggcctggcg ccggaagaaa ccgcttacct gggagacgat
    14221 attgtggata ttcccgttct gaagatggcc ggattttccg ccgcggtggc cgatgcggtg
    14281 gatgaggtga aaaagcatgt cgattatgtc acgctttgcc gcggcgggaa gggagcggtg
    14341 agggagatct gcgagatgct gcttgaggcg ctgggcttct ggccggaagt tgccagacgt
    14401 tatgactttg acggacccga atagctttac agggaagaga gcgagtgata tatcaaaccc
    14461 atgggttttc aaagaaaagt aaaaattgca gcgattacgc tgatgattct tgtacttgcg
    14521 ggcgcggtcc tgtttgccct tctgcgggcg accggcggca aaaaagagac gattgtcaag
    14581 atcctgcctg acgaagcgga tgtccgcatc gcggatttct tttatacgga agtcggcaag
    14641 gacaatctcc gctgggaggt gaaggcgaaa accgcccagt atcagaagaa gaggaatctg
    14701 gccctgtttg atcaggttca gatgaaactg atcacgcaga aaggcaggac ctttgtgatg
    14761 acgggcgacc agggcgagat gcggaccgac agcaaagacg tcgaaatccg gggacatgtc
    14821 gtggcgacgg ccgatacggg agaaaaattc tccacggagg ttctgcgcta cagcgacgct
    14881 cggaaacaaa tttataccga cgcctccgtc cggatggaaa cgagccgcat gaaaattcag
    14941 ggagtcggcc tttccattct gatggataaa ggggagcttg cgctttcctc cggcgtcaaa
    15001 gcgaaaattt actgatggga ccatgttgat gaaaaaaata gtcctgatca cggcggggat
    15061 tctggtcgcc ctgaacctac aggcctgggc gcaaaccggt ttgcttcaga aaaacacgct
    15121 tcggaaaaat gagccgattc agattgtttc cgataaaatg gaagcttttc aggccacccg
    15181 aatggtggtg ttttccggca atgccgtggc gcagcagggg gatgtccgga tccaggcgga
    15241 ccgcctgtcg atttactaca agaactccgc cgcccagagc gaaaaagtcg gaccggacgc
    15301 ggagcagacg ggcgaccttg aaaaaatcgt cgccagcggc cacgtggtga tcacgcaaaa
    15361 agatgtgctg gccacgggca atgaagcggt gtattatcag gacacggccc ggttcgtcat
    15421 gacgggaaac cccgtcctgc ggcaggaaaa aagcgtgatt aagggctgca gggtcatcat
    15481 tcaggtcgat gagaaccgcg ggaaagtgga gcagtgcgca gacgaaaatt cgggaagggt
    15541 cacggccatc atccaccccc ggaagaaaaa ataatcctgc gtgacgggct cccgagaggg
    15601 aaaatgagtc atttatcggc caggggactg gttaaaattt acaacaaaag aaaggtggtc
    15661 aaccacattg acctgacgat ttccccgggg gaagtggtcg ggcttctggg gccgaacggc
    15721 gccggaaaaa ccacgacgtt ctacatgatg gtcggactgg tcaagccgga cggcggtcaa
    15781 atctttctgg acgacgagga gatcggcgcg tatcccatgt accgccgggc gcgaaaggga
    15841 ttgaattacc tgccccagga gccgtcgatt ttccgaaagc tcaccgtcga ggaaaatatt
    15901 ctggcgatcc tggagacgct tcagatcagc gaagaggagc gccggcgaag gctcgaggag
    15961 cttctgggcg agctggacct gacaacgctc gcgaaaaacc gcgcgtattc cctgtccggc
    16021 ggcgaaagaa gaagggtgga aattacccgc gcgctggtga cgtctccgaa atacattttg
    16081 ctggatgagc ctttcgcggg catcgatccg ctggcggtgg ccgacatcca gaatattatc
    16141 gagaagttga aaagcaaggg aatcggaatc atcatatccg atcacaatgt ccgggagacg
    16201 ctggcctgct gcgaccgggc gtacatcatc aatgagggcg cgattctggt ggaaggccgg
    16261 ccggatgtaa tcgcccggag cgaactggcc aaaaaatttt atttcggcga ggatttcagt
    16321 ctgtagcgcc ggacggatac gcgatgttca gccatggctt ttgaattacg gcaaaactta
    16381 aaactttccc agcaactgat catgacgccg cagcttcagc aggcgatcaa gttgctgatc
    16441 ctttcgcgcc aggagctggt ggacacgatc aaccaggaga tggaggaaaa tcccctgctg
    16501 gaagaagtgg cgccggacga tgaaagcgcc gaggaactcc acgccgaagt cgcggaggat
    16561 gtccagtccg ttgaaaaaga ggaaagcaag attcccgagc acacggccga gctgaccggc
    16621 gaaggggacg ggcgggaaga attcgactgg aacaattatc tggaagatta cggtccggtg
    16681 ggcgtttcct atgaccgggc cgaaggagac gccccgacgc tggacaacgt cgtgacggaa
    16741 agcccctcgc tgaccgaaca tctcatgtgg cagatgaagc tctcgccctt caacggggaa
    16801 gaggtgcgcg tcggggagca gatcatcggc aatcttgatt ccaacggtta tcttcgcgcc
    16861 accgtggagg aaatcgcccg tctggaaaac gtcgccgaag agttcgtgga aaaagtcctg
    16921 aaaaaggtgc aggaatttga tcccgcgggc gtcgccgccc gggatcttca ggaatgcctc
    16981 ctgattcagg ccggcatgtt cgcccgggaa aacccgctga ttgagcccat tatccgggag
    17041 cacctcaagg agctggagct gaaaaattac ctgcagatcg cccgcaggct caaagttccc
    17101 ctgaaggaag tggaaacagc cgttttgctg atcagcaaaa tggaccccaa gccgggcgcc
    17161 gtctattcgg aagagaaggc ccaatccatc attccggacg tgtatgtggt caaatcgggg
    17221 gatgactata aaattattct caacgatgac gggctgccgc gtttgcggat cagcaatttt
    17281 taccgtgaaa tcatgggcgg cgtcggcgga ccggactccg cggaaaagga aaacggccgg
    17341 aaatacattc gggaaaaggt gcagtcggcc acctggctga tcaaaagcat tcagcagcgg
    17401 cagaaaacca tttaccgtgt tgccgaaagc atcgtgaagc accagaagga attttttgac
    17461 aaaggcatcg actatttgaa gcccctggtg ctgcggaaca tcgccgacga tgtggaaatg
    17521 cacgagtcga cgatcagccg ggtggtgacg aacaaataca tgcattctca ccgcggcatc
    17581 tttgaaatga aatatttttt cggcagctcc attcagagaa cctccggtga gaatatcgcc
    17641 tccaaaagcg taaaggagga gattcggcag atcatcggca aagaagacca gaaaaaaccc
    17701 tacagcgatt gtgaaatcgt gcggctgctg aaggcccgga atatcgacat cgcgaggaga
    17761 actgtggcaa aatatcgcga aatgactggt attttgccgt cgtccaagcg gaaaaaatat
    17821 ttttgacgca ggcgttttgc agcgggaaac catcaaggca aaaagtaaaa ttccggttga
    17881 catttggttt cgtcgccact atacgactaa cctttgttgc cggccgtgaa ggcgtgtttt
    17941 gaatgttttt aaacggccta aactcaagag ggaggaaaaa ggatgaatat ttcggtgact
    18001 ttcagaacgg gcgaagggga gaattggcaa aaggaatacg cggaggaaaa aattgccaga
    18061 ctgaaaaaat atctcgatgt gcctgccgac gtccatatga tcctttccac ggaaaaattc
    18121 cgcaacatgg tggaaattaa tctgaccgcc aacggatgga atgtaaacgc gaaagaagaa
    18181 gccaaggaca tgcgcctggc cgtggaccgc tgcatcgtga aaattgaaaa ccagctcaaa
    18241 aaacaaaaag aaaaaagcag gcagcacaag cccaaaagca tccgtcacgc cggcgacaaa
    18301 gccctggaag gcgcggagga ggaagatgcc gcctccaaag tggtcgaaac ccgcaaaatc
    18361 attttaaaac ccatgtctct ggaggaggcg gttctcgaac tggagagcgg caaggcgcgt
    18421 ttcctgatct atcgggacac ggcttcggag aaagtgagcc tggtgcaccg cctggatgac
    18481 ggccagctcg cgcatattga aacaaacagc tagttgccaa aagccgggtc cgcggcatga
    18541 aaatgcgccg gcccgtcggg atcatggatc tatgagacgt caggacagcg tcccggattt
    18601 caaatacagg tgtggcgcat gcgactggat caaatattta aaatggaatt tctcagcgaa
    18661 aatctcctgt ccgacaccaa agaaggagtg cttgccgagc tgatcagtct tctgatcgga
    18721 agcggcttga agattgaccg ggcaaaggcg cttgatgtgc tgcagcagcg ggaaaagctg
    18781 ggctcgacgg gcatcggcga cggcgtggcc attccgcacg gaaaggtttc cgaactgcag
    18841 gacatggtca ttgccttcgg gagaagccgc cggggcgttg cctttgacgc cattgatgga
    18901 aagccggtcc agcttttttt tctgctgctg gcgccggagc agtccacggg gcagcacctg
    18961 aaggctctgg ccagaatttc caagatgttg aaaaccccca actttcggaa aacgcttgtc
    19021 gatgcgaaaa acagagccga tttgtacaaa gccatcgttg agcaggatgc gacctgtccc
    19081 ctctgaacgg gatgtgcctc atccggaggc ttccgcacgc gaatggtttc gggactatct
    19141 gccgtatctg ggcattcgaa caatttccgg cccgcgcaat cacaaaaatc cttttcccgc
    19201 cggcgcggtc cagtgtctgc ggagtccgac cgcattcccc cggcgggaag ccgcgattct
    19261 tctttttccg cccgagacgg caaataaact tctggcgctt tccgggccgt cccgcgagga
    19321 atatttttcc cgcatggccg ggcgccgtcc ggttttgctg attttttcgc aaagcccgtc
    19381 cctgccggcc gtgatccgca atcaactgcg gaaccactgt ctttgggcgg ccgcttcccc
    19441 gcttcacgag aatctgctgg ccagccgcat ccgttcaatc ctcctggaga aaattcatca
    19501 atgcatggcg atgcacggcg tggcactggc ttgcggggac cggggcgtgc tgcttaccgg
    19561 acccagcggc atcggcaaaa ccacggccgc gctggaaacc gcccgcgaag gctgtttctg
    19621 gatcgccgac gatctggccg tgattaaaaa tgttcgggga cggctgatga tttccggcca
    19681 cccgagaatt caaaaatatg tccatgcggg ggagaaggga atcgagtttg caaatcgggt
    19741 gctgccggcg cggcggacaa aacaaagggc ggagcttttc ggcattgtcg atgtctgccg
    19801 gagtgccggg aagaaaatct cccggcccat agagatccgg attttggaaa agcgcctgcc
    19861 gctcgttcgg gtgtgcattt cccggactgg atatttgcgt caaaatctgt taaccagagc
    19921 catgcaaaaa ctgaacgagg tcggataatt gaaaaatctc caggtggtga tcatcacggg
    19981 attgnnnnnn nnnnattgtc cggttccgga aaaagcaccg cgctccgggc gctggaagac
    20041 atcggctttt tttgcgtgga caaccttccg gttgtgctgc tgccgaagtt tctttccatc
    20101 acgctgcagt cttctccgaa gatcaggcag gtggcgatgg ttatggattt gcggcagaaa
    20161 agctttctgg agaagtacca gcgcatcttt gaacgcctga aggaaaaggg ctaccggatc
    20221 aatattctgt ttctggaagc cggggaagag tctctgctgc gccgcttcag tgaaacccgg
    20281 cgcagccatc cgctctccga aaagcgctcc atcatggaag gcattcagga agaagtgaaa
    20341 aagctttcca ccctgcggca gatggccgac accgtcatcg acaccaccac ctccaacgtc
    20401 caccagctca aggacaccgt gcaaaagcag tttctgcctt cttccacccg gaaaaaaatg
    20461 atgatcagcg tcatgtcttt cggataccgc tacggcctgc cggcggacgc ggacctggcg
    20521 atcgacgtgc ggtttctgcc aaatccctat tacgtggaaa agctgaaaaa cctaaacggt
    20581 catcaccgcg atgtggagaa gtacgttttg aacaacgagg aatgcaaaac cttcatgaac
    20641 aaagcggccg acatgctgaa cttcatcatt ccgctgtatg aaaaagaagg aaaggtgcgg
    20701 ctgaatgtcg cgttcggctg cacgggcggc aagcatcggt ccgtcgtcat ggccaatcag
    20761 ttcagccttt tttttcaaaa gcagaagttc atcgtccaca ccacgcatcg cgacatcaat
    20821 aagagttagg caagcgcgct tcacgccgcc tgtggcggcg actgatgagg gagtccgttc
    20881 ggggaacttg ctcagaagcg tgcaggcaac gggcgacagg ctataggaat ccgttttatc
    20941 ccgcctatca ccgattgccc ctcgcccata gccttataga ttcatctttt tcatcacgtt
    21001 gtcttcaatc cagtgcgggt cccaccattc ctgcgggttg acaaactggc cgccgaccag
    21061 aatgctgaaa tgcagatgat cgccgccggc cagtccgctc aggccggaga ggccgatctt
    21121 ttcctctttc tttaccggct tgccgaccgc ggtttcaatt gacgagagat ggccgtataa
    21181 actgaacagc cccaggccgt gatcgataat cacggcattg ccgtaaatgc ccagcgcgcc
    21241 cgcgaagaca acgattccgt tgttggcggc ttcgatgggg gcgtgtgcgg ttgaggcgag
    21301 atccacgcct agatgcacgg agttgccaaa gggtttgccg tccaccaggt aggtgcgctc
    21361 gtcgccgaac agggccatgg gcgaggcatt gcgcatccgg tgaaacgcgc cttcccataa
    21421 ttttttattg acggacttgc ggcagacgga ttgaatggtt tttgagtttt cctcccgcag
    21481 cgtggtgttg acatagccga agacctccac cggcgttttc ccctgcagct gcggaatcga
    21541 tccctggaat tcgggcatga ttttctggag aaaggtttcg ctcaagttca ctttatccgc
    21601 gcggaatttc ttcggcttga tcgtaaaggg catggacgtt ctggcttcgt tgccggcgct
    21661 gtcccgggcg aagatgacga ttttggttga ggcggatgcg ccggtgccga cggcgaaggt
    21721 tgccaccgac gtgggttggc cgccgatcac gatgttgtat cccgggtaaa accggtcgtc
    21781 cacgaagacg ccggtcagcg ccgcgggctt ggacgtccgg tacgggatga agccggcgcc
    21841 tccctggttg atgtagttga cggggctcat tacgtaaacc tgaggcggca tcatgtcaat
    21901 ccggacctgc tcggagatga cggtttcatt tttaaagagg gaggcatccc cggccgtgat
    21961 gcggacgacg gccggtccgt tgttgagctg gagcaagagg ggctccaccg taaggcgaag
    22021 aaccttttgc ctggtgctcc gggacggaaa agattcctgt gccagaagac gcggcttgtt
    22081 gtcctgaaca atttccacct tcaggcccga caggccgctt cgctcgtcgg aaaaggcgat
    22141 gctgatgtcc ttttttttgc cgatggccgt gatgtccgaa tccagccgaa tcgcgggttt
    22201 gtcccgatcc agccagccgg caaaataaag ccagcccaat gcccccgccg ccagaacgat
    22261 gacgaccgcc gccgcaataa tccccgtctt tttcatttcc gaagctcctt gattaaatcc
    22321 tgatattgaa ttggcggttt tcataatggc ttttgccgcg gtttgcaagg aaaatatact
    22381 ttttgcgcgg gcgcatggaa agactttata ccttgatctt gctgggaaac gctgttatat
    22441 tcgccgaaaa aacagcgaag tgaaagacaa atgctgaaaa ataagaaaat tgttctgggc
    22501 atcaccggcg gtattgccgc ctacaaatca gcggagctga cccgcgcttt catcaaagcc
    22561 ggcgccgagg tcaaagtcat catgacccaa agcgcgacgg agttcatcgc gccgctgacg
    22621 cttcgaaccc tttcccggaa ccctgtctat gccgacatgt ttgaggacgc ggagccatac
    22681 gacatggccc atatcgcgct tgcggaatgg gcggacgcgt tcgtcgttgc gccggccacg
    22741 ggaaacgtga tcggcaaaat cgcctccggc atcgccgacg accttctgac cacaaccctg
    22801 atggctgaaa acaggcccac gctgatctgc ccggccatga acgacaaaat gctggccaat
    22861 gccttcgtgc aggagaacct tcggaaactc gccggggccg gctatcgcgt gatgcaaagc
    22921 ggcgtgggcg agctggcctg caacgcagcg ggcgccggac gcctgccgga gattccggag
    22981 attctggacg agatggaagc gcttttaacc gtccaggatt ttgccggaga aaaagtattg
    23041 gtgacggccg ggccgacgca ggagccgctg gatccggtga ggtacatcac caatcattca
    23101 tcgggcaaaa tgggctacgc gctggcgaag gccgctcatc agaggggcgc gcaggtgacg
    23161 ctgatcagcg ggccgaccag gatcgttccc ccgaaagttg aaacggtcgt ttttgtgcgc
    23221 accgcgcagg aaatgcaccg ggccgtcatg gacgcctgga ccaaagcgtc ggtcattatc
    23281 aaagccgccg cggtggccga ttaccggccc cgggttgcgg ccggcgagaa aatcaagaaa
    23341 gaccgggggc tgtccggcat cgagcttgtg cgcaatcccg atatcatcgc cgacatcggc
    23401 gcgaaaaaga agaaaggcgt cctggtggga tttgccatgg aaaccgagga cctgctggac
    23461 aacgcgcgcc ggaaaatgat ccggaaaaac atggacatga tcgtcgccaa caatctgcgc
    23521 gacgatggcg ccggttttga aggcgacacc aacatcatca cgattcttga ccggctcggt
    23581 cataccgtcg ctctggataa aatgaccaaa caggatgcgg cgcaccgaat cctggatcat
    23641 atccgctctt ttaaatccaa gaaaggaccc gccggaaaaa aatgacgcaa tcctttccgc
    23701 catcttcatc cgacgagatt cggaatgaac tgctgtccgt ggtgaaatcc gtcagaggac
    23761 aggtgctgga cgcagagcgc ctgtatgtcg ccctggactg caaaagacct cagccggccc
    23821 ggaaaaacac gccggacggc agtgatcgcg gcgaggcgct tcactccctt tccctggaag
    23881 ccgcgaactg ccggcagtgt ccgctcgcgc aaacgagaaa aaatgtcgta tttggcgccg
    23941 ggagtttatc ggctcaggtg gtttttgtgg gcgaagcgcc gggcgcggac gaagatgaac
    24001 agggccagcc ttttgtcggc aaggcgggac aattgctgac ggacattatt gtgaaaggaa
    24061 tgaaactgac gcgcaaagac gtttatatct gcaatat
//
LOCUS       DAOSIM010000006        23563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_2397_length_23563_cov_8.321210, whole genome shotgun sequence.
ACCESSION   DAOSIM010000006 DAOSIM010000000
VERSION     DAOSIM010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 23563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23563
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2397_length_23563_cov_8.321210"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLR47_00600"
     CDS             <1..298
                     /locus_tag="PLR47_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11459.1"
                     /translation="ELLYKHDKVRDACVIGVPDEAQVQAVKAFVVLKNPADAGPAAEK
                     ELIHYCRENLIKWSCPRSIEFRTDLPKTLVGKIAFNTLEKEEVEKLKKEGKFAG"
     gene            complement(395..805)
                     /locus_tag="PLR47_00605"
     CDS             complement(395..805)
                     /locus_tag="PLR47_00605"
                     /inference="COORDINATES: protein motif:HMM:NF009806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine synthase"
                     /protein_id="HOG11460.1"
                     /translation="MLVRTINDITGTDREVWAENKNWVSRRLLLKKDGMGFSFHETII
                     FAGTQTHIWYKNHLEAVFCVEGEGEVETPADGKIHHIVPGTIYALNNHDEHLLRASKD
                     MRLLCVFNPPVTGAEVHLEDGSYPPAENQGKVPS"
     gene            complement(821..2074)
                     /gene="ectB"
                     /locus_tag="PLR47_00610"
     CDS             complement(821..2074)
                     /gene="ectB"
                     /locus_tag="PLR47_00610"
                     /EC_number="2.6.1.76"
                     /inference="COORDINATES: protein motif:HMM:NF006733.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HOG11461.1"
                     /translation="MKTIRRLESEVRSYSRSFPVLFEKAKDAFLFDADGNRYIDFFAG
                     AGTLNYGHNNDHMKKKLIEYLETDGIVHGLDMATVAKEELLLKMESIVFKPRNLDYKI
                     QFTGPTGTNAVEAGLKLARMVKKRSNVISFTNGYHGLTMGSMSVTANAYYRDEAFINR
                     SDVSFLPYDGYLGKDVNTAQYMRRFLEDNSSGVDIPSAVILETVQGEGGIRVASDEWL
                     REVESICREFDILLMVDDIQVGNGRTGHFFSFERSGIKPDIVTVSKSFSGYGLPMSLV
                     LFRRELDIWKAGQHTGTFRGNNLAFVSASVALDYWKDDRFSKDIFRKGTILSDRLHAI
                     CARYPQLNAEVRGRGFIYGMAVDPPEIAREISMECFKNKLIMELAGARNNVIKFLAPL
                     IIPDEVLMEGLDILDASIGSVLKNR"
     gene            complement(2092..2592)
                     /gene="ectA"
                     /locus_tag="PLR47_00615"
     CDS             complement(2092..2592)
                     /gene="ectA"
                     /locus_tag="PLR47_00615"
                     /EC_number="2.3.1.178"
                     /inference="COORDINATES: protein motif:HMM:TIGR02406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="HOG11462.1"
                     /translation="MKKDPTDMYFRRPEVADAPFIEKLVKDSPPLDLNSLYCYLILCA
                     HFSRTSVVACDADAVCGFISAYLRPDEEDTLFVWQVAVDKKYRGKGVARSMLSSILNR
                     PTPCPPRYLETTVSPSNIPSKALFSSLAASLGVPLVQSVLFSADDFGDQRHEEEVLFR
                     IGPFDL"
     gene            2889..3239
                     /locus_tag="PLR47_00620"
     CDS             2889..3239
                     /locus_tag="PLR47_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11463.1"
                     /translation="MKKLFYKISIYLSRRLGLWFFRVFSWFIATGYFFLFPPRVRDSL
                     QFYRALFPGRSFGYHLYCVWKQFHNFTGVYVHRFIPWAAEEAKFTREGWEHLDEAVAK
                     KTGAIVVMSHIGNW"
     gene            3336..3911
                     /locus_tag="PLR47_00625"
     CDS             3336..3911
                     /locus_tag="PLR47_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOG11464.1"
                     /translation="MTEQNAQPQPLKTIADPACGTGGFFLAVYDCISDPQNFILNKEQ
                     KLFLKNSTYYGNEIVAAARRLALMNMFLHNIGDIDAETFILPTDSLVADTGLRVDYVL
                     TNPPFGKKSSMTFTNEEGEQEKDDLTYNRQDFWATTSNKQLNFVQHIRTMLKTTGKAS
                     VVVPDNVLFEGGAGEIVRKKLLETTDLHTIL"
     gene            3979..4203
                     /locus_tag="PLR47_00630"
     CDS             3979..4203
                     /locus_tag="PLR47_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11465.1"
                     /translation="MASGKNPEGRRRKFTYDEIVARDKTSLDIAWLKDKSLADLNNLP
                     HPDALANDIIENLESAVASFKEIMETINGK"
     gene            complement(4243..5829)
                     /locus_tag="PLR47_00635"
     CDS             complement(4243..5829)
                     /locus_tag="PLR47_00635"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOG11466.1"
                     /translation="MENVKKPSGEMILSFDAGTQSIRAALIDASGNIAQIVKRRIEPY
                     FSPEPGWAEQHADYYWKMFCEACRELLDKSDKSSREAIVAVTLTTQRATMINLGEDGR
                     PLRPACLWLDQRKANAEEIVPSFAKPLLKSIGLWDRLNNAVQSCPSNWIQQHQPEIWR
                     QTHKYLLLSGFFTFMLTGEYADSAACNVGYLPFDNKTYQWAGRFDLKWRLFPIEKEKL
                     PRLVKPCEVLGRITARAGEETGIPAGLPVIAAGTDKGCEMLGAGCLTPDTGCLSFGTT
                     ATFNTTTTKYIELLSMIPPYPASVPDTYYTEVMIYRGFWMVSWFRDEFGMLERKIAEK
                     NNIAPEKLFDQLIEKVPPGSMGLILQPYWSPGTNTDPYAKGSIIGFGDVHNRAYLYRA
                     ILEGLVYGLREGAELTQKRTGTAVSRLRVSGGGSQSDMAMRIAADIFGLPAERPHTYE
                     TSALGAAIDAAVGLGLYPDFPSAVKAMTRVKEVFEPVAENHRIYDRLYREVYLAMYGR
                     LRPLMEKIQDITGYPSPSRQ"
     gene            complement(5817..7529)
                     /locus_tag="PLR47_00640"
     CDS             complement(5817..7529)
                     /locus_tag="PLR47_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOG11467.1"
                     /translation="MTATNFRPDWTEKAPPAGTYRSIFKYGNPNHFKHPSDAWYAMLK
                     QDFHLTDEDFSAKSGEGLNLVSLNRPSALKPGQIEALRAIAGVENVACDDYSRLKYSS
                     GKTTEEMLELREGIVREVADVVVHPRDKHDVRKIVEYCDRELIPVTVFSGGSSVNFGC
                     RPSRGGVSLAMSTHMNKLLEINEINQTARMQPGMFGPACEDALNRAPQLWQTKHRYTC
                     GHFPQSFEYSTVGGWVATLGSGQASTYYGDACDIVFSQEYVTPAGTFKTLDYPATATG
                     PKVNDIMKGSEGAFGILVEVTMKIFRYLPENRTYFSFMYPSWEKAVEASREIMQSEFG
                     RPAVYRISDPEETDRGLKLYGIPDLADRFLTLRGFKPLCRCLCLGNCEGDKDYSRLVA
                     KKIKSTARRHGAVSLSGYAARKWEQSRYTEPYMREDLGDYGILIDTLEAAVTWDNLHR
                     LHQGVRAYIKSRPDTMCMTHASHFYPQGTNLYFIFIIRMNDPGEFRKFQRGIIDSIQK
                     YGGSLSHHHGIGRMMAPWMETHLGKEQMAVLRALKNHFDPRGIMNPGGLLELKSSGEK
                     RWKT"
     gene            complement(7559..9205)
                     /locus_tag="PLR47_00645"
     CDS             complement(7559..9205)
                     /locus_tag="PLR47_00645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOG11468.1"
                     /translation="MLRFIENYTNEKFDVLVVGGGITGASVAYEAAARGLKVALLEKK
                     DFSWATSSVTSKLIHGGLRYLANGEIGLVRESLRERRVLENIAPNFVYPIPILMTHYK
                     KPLKNSKWVVKAGMLMYDALSYDKKYTWDASKKIPRHKTISRRTVLESEKNVRPAGLT
                     GASLFYDCMSIFPERLTLAFIKSAAACGAKVANYAKVESFLTDAQKNVAGVRVKDLLT
                     GKTMAISSSVTINCAGPWADLLLGLAESNSAGGAALRRSEGIHIITKKKLVSGKYVVA
                     CMTPKGRHFFLIPWRGHTIIGTTDKPFVGNPDAYHVTKAGILELIDEVNGSFGDGTLS
                     YADVVHTYGGLRPLVEGQTEETYTSSRRYEILDNKENGLNGLITAEGGKFTTSRNLAE
                     KCVDLVEAKLGRPRAKSKTARHHLAGCRIKNLNFFMEEIHQHNTNGFCRNTLDYLGRC
                     YGTDYRAITKLAHENKELAEQINEDGEIFAEVVYAVREEMARTLSDIVLRRTGIATLG
                     NPGEKILRKVAVVAAKELQWTPERMEREIAGTINTLRLPQ"
     gene            complement(9208..9930)
                     /locus_tag="PLR47_00650"
     CDS             complement(9208..9930)
                     /locus_tag="PLR47_00650"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOG11469.1"
                     /translation="MTAKKVLAQPVSRARLNEEIVTVLQNQILSGKLAPGDKIPPERE
                     LAETFQVNRATLREALRKLEILELVEIRHGNGLYVKNYLDSGNLDLIRAAANLDGREE
                     VVLDILEARRYLMPQIAWLAAARRSNEDLLELERVTGSEELTMLERDIKVHQIIARST
                     HNLLCTIGLNFFNQIFRDYGPLYFNDEKNAQRSRKFHRDIYEAIKSQKPIEARKIMRE
                     IMIYAEEVVKAGLSGNKKQKES"
     gene            10148..11029
                     /locus_tag="PLR47_00655"
     CDS             10148..11029
                     /locus_tag="PLR47_00655"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG11470.1"
                     /translation="MKKLFYKISIYLSRRLGLWFFRVFSWFIATGYFFLFPPRVRDSL
                     QFYRALFPGRSFGYHLYCVWKQFHNFTGVYVHRFIPWAAEEAKFTREGWEHLDEAVAK
                     KTGAIVVMSHIGNWELAAQRLQEKGLPVMLYLGAKHKEQIEKMQKEKLAQTGIRIVTT
                     DEKEKSPFALLEGVGFLREGGIVSMAGDRLWGDQTSVEVDFLEHKVRLPDTPHLFALM
                     SGAPLMTFFVHEKAPGHYHVQVSKGRKVAAAARAERKKAVQESVQAYADDLARFAAAH
                     PFEWHHFEPFLGEKIIP"
     gene            11114..11512
                     /locus_tag="PLR47_00660"
     CDS             11114..11512
                     /locus_tag="PLR47_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11471.1"
                     /translation="MNVREVPQDITYYEGKKRACWALNDEGKYVIVPSSGWEVEAVVN
                     GLAVEEMAASLEATRQAVLAGVKSPLCYYMELRQMTPEILGKTARVAAFRVRRHFRPD
                     VFSKLGPSVLDRYARALAVTLDELKTVPQT"
     gene            11532..12566
                     /locus_tag="PLR47_00665"
     CDS             11532..12566
                     /locus_tag="PLR47_00665"
                     /inference="COORDINATES: protein motif:HMM:NF027495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HOG11472.1"
                     /translation="MTTEVTAAGKPFVHVHSAHCESGVTAALFRDKGVEISEPMIFGI
                     GSGIFFGHLPFVKMGGVTISTFRSQPGAVFRKSAKRLGGKFKTARFRNPKKGMDELRR
                     VLGTGQIVGLTTNIYWLSYFPNRFRFQFNGHNIVVLHEIENGFRVSDPMLEEPVDCPA
                     EDLERARFARGPLESRGFMYYPQSVNPNPDLRAAGLQGMKDTCKMMLKIPLPIFGIRG
                     MRYLAKKIIRSPEKLGFMGACRELSSVVRMQEEVGTGGAGFRYMYAAFLQEAAELFAS
                     KELARLSGEMTAIGDIWREFAVVSARIIKKRGQTEETFEKAGGLMLICAGREEQLFKD
                     LAKAAGKLKA"
     gene            12563..13282
                     /locus_tag="PLR47_00670"
     CDS             12563..13282
                     /locus_tag="PLR47_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG11473.1"
                     /translation="MNALDISSVTFCYDGSPAPALRDFSMAVADGRTHALLGPNGAGK
                     STLMRIITGQLQGQTGGVSVYGLRMPDSKALSFIGCAPQPISLYPSLTARENLRFFGA
                     MAGMSKDQIASRSDWALEKTGLAESSEAAVASFSGGMQRRLNLAAALLHSPKLLLLDE
                     PSAGVDPQSRNHMYDLLMELNAGGVTILLCTHMMEEAQRLCSGLTLLDRGERIYDGRM
                     SDIGNLEEFFLKKTGRGLRDL"
     gene            13272..14393
                     /locus_tag="PLR47_00675"
     CDS             13272..14393
                     /locus_tag="PLR47_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG11474.1"
                     /translation="MIFKQLIELVRKEMLVLVRDRQTILLLFLMPVALIFFMSLALEG
                     VWADKLVGRKISMVVDNQSRSYQANLLAGKIKAHALIVPVERPAGADVDRIFAEGTAQ
                     AVVTIPPGFEEGDRPVEIFFDPVMDASYRIAARSLITSLVVEVVMGVDHLEAVVAGLV
                     VERSGPNRELPSPLQQTVPAYAIFAMFFIAIPMSVGFLREKKDGTLQRLFTYPVSANL
                     VTLGKITPYFLINVVQFVLMMLVGVFVMSRFITPAFFAGQHPWHMLPVTLVVAAATTG
                     FGVLVAATAKTPEQSSTLAATGAILMGVFGGIMVPHAVMPPVMKKLAMISPMFWAHQA
                     YLDIFLRDAAFGIILPRLIILTLFAGICFYIAGRKVRWI"
     gene            14384..14695
                     /locus_tag="PLR47_00680"
     CDS             14384..14695
                     /locus_tag="PLR47_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11475.1"
                     /translation="MDLEQAYRDLNVTSREDLLFKLKELVIRACEIRDVRTEDVPTDV
                     PFIGGPGPLKLDSLDAMEIAMELRYTFGVELKNASTAAKAMQNFDTLADFVIAAPKVK
                     K"
     gene            14692..15867
                     /locus_tag="PLR47_00685"
     CDS             14692..15867
                     /locus_tag="PLR47_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOG11476.1"
                     /translation="MRNGNIHIRGWGAVTALGWGADQGARRLIAGDVHPAGVCGSAHL
                     VNREFKGFEVACDGNPLEKAMKMLDASIAEAFERGGLSAPEIAESALLVGTTGGIFIR
                     NEHEFTESVRRNPGKESPPIVCRNRGPGEVADVIARRYGIRGLPMTFSMACVSSSHAL
                     VVAADLIAQKRIRRAVVVGFEPLLNLTLQGFASLLLYDPDECRPFCNRRMGMQIGEAA
                     SCVILEESDSAAAGKSGFAFAGGHLYNDNKSLTSAGTDGEMVRGVVSRALLKAGTEPG
                     EIVAVKAHGTGTRDNDLSEGRGLAKVFEKHLPPVVSLKATIGHTLGAAGTVETALWLS
                     CLEAGVIPKSFGFAETDPEIGFAPSQENLPAAAGAYLFTYFGFGGTCTAYVIRKMAS"
     gene            15871..16992
                     /locus_tag="PLR47_00690"
     CDS             15871..16992
                     /locus_tag="PLR47_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOG11477.1"
                     /translation="MTEKLKHSSLPVIAAGYLGPALVPNADGAWEEAKLPANPDDLVQ
                     KMMGQPMRRAGRFIKLVCCGALACMSRVPEETLRGRSIGIFLATGLGNIDEILPFITQ
                     VFKHDGGFPSPNQFANSVSNAAAFFLAKACGIKGVVLTVSQEELSFESALWLAQGYLA
                     SGEIDLALVGGCDVFTPSVEDYRERLNLVPGRCRRAPLGEGSAWFLLGGDGEAKIGDI
                     LSVEISSGLADAESLPSDDLLKQCEKLFEEFRNNMESPDNGANSALLSFPRKRESRPS
                     DGLVDSRLRGNDREEGFFEDDKISKVFLPGFRLANLDEASWKKSGWDMDDYLIRCGIS
                     STASACGLARALLSRQSSFLVHGNLSSSGRPALMSAVTK"
     gene            17170..18615
                     /locus_tag="PLR47_00695"
     CDS             17170..18615
                     /locus_tag="PLR47_00695"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG11478.1"
                     /translation="MVLRQQNKRIVLVHPRGSNWFPGRRDITDIANRMVPQGMLSIAA
                     WLTRDGHDVFVYDCLGPGAPVDTNRQAHDILAFDPQIVGFSATTSSFPDAADLAQKIK
                     DSRPDTVTVCGGVHVSALREILLAAYPAFDYLAAGEGELTLGELAAGVDPSEIAGLIR
                     RENGIAVANEPRKHITNLDDLPFPAYEKLRGFPRDYHLPLFSYKQTPGATMITSRGCM
                     YSCSYCDRSVFQKGFRYNSAAYIYDHMKYLREKFGARHVNIYDDLFTANRKRIVELCE
                     KLARQPLGMNFNCAVRVGYTDDDLLGMLKEAGCLMVSLGIESADPDMLARHKSGVSLD
                     DVRETVSRIQSAGLRAKGLFMMGLPGETEASIRRTSDFIISLGLDDMNMAKFTPFPGA
                     PLWGAIREEGTFTEDWRLMNCLNFVFIPRGIESKEKLDHLYNEHVKRFYSDPAWRRRF
                     RGRIWEHRHSMLYLLRHLPSFWSAKNQFEPD"
     assembly_gap    18688..18787
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18926..23527)
                     /locus_tag="PLR47_00700"
     CDS             complement(18926..23527)
                     /locus_tag="PLR47_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11479.1"
                     /translation="MMDGGLYPEEPVVTGIGIAPENLYTSTAQTMDYSGMNLAATLGT
                     GGSIWTDNQSLETLFIVKEGQGQPWGIYRQGFAGRFNPDESGQTTWSGQTGGIGTFGY
                     YYDSNYGNMGDTGYWLADASGNWWGDGPVDGALNNGWFITGTKSGNLSGNFLGVYEGQ
                     GNTWQATGLGTWSGKPLAYSAKVLSGSWGYYNTTPGSEGLVNDGYPIAGLTGGFSPLF
                     GELNADVMGLGTYTRNQNSPLFNMLLTNADGGPETNPFHVYYGGSWFPAGIDYPEWFE
                     GKMMGFYGRNWDGTKYNEIGLITSGQLIDENGEDSGFSVWGDLYSNAGMWKIPDDEDQ
                     GTLTHTVKPGTASSFSIVESEDWSPGAKVDGDLQGTMRTKSAHIDGIEDWGVWMSAMG
                     GSYPDTLPERWASYTGWKETNESGKPSYSIASVDGYAYNDGALWGGVFGRFLNPDNMG
                     VFTGDMVGITNNGNWQAMGIGSFEVTKALAFSSVFSGQTFKTAKGTLNINIYAMPGVN
                     SNMGIFQGVDYETAYFRAEGDTSGSLVKYGWQWENARNRYNNQRTDLIYYPNDSLQSL
                     RVVNGVPVEVQGSTSASSSAWGFWPDLHQSPPVSPIVANSNFIIVNDAFPFTGQLAVN
                     PANMEFAKIFSQQVDNWYTTVRLIPDPEVPLAPTFEGIMGGYAKDGDAGRTLWTSTED
                     NPMGLALMGEHAEFEAPVTMATGFGSSYNGTSTTPDGGAYFGFLTNRVSANRSTRGVF
                     YGFYISPESEAGFLKSTNIAGMSFAGLNMWEADGDIYSEMLGTISMPASDLMTNIQLG
                     AMSGNIQYGGAGGNFVSGGYLKHWAMGNGYTASIKDHSSNGIFINASSAGEYSNPEGS
                     TNWQADFAGYGTFGNFGDAGQPGAFSDYGIWHMNAGGADASWAANEIYAPVSGEFLTY
                     TKKGNLSGTFLGNYSGTTSGIWGGTASGVYQKTANVAFSSGMAGKVYANNLTKWISGT
                     NSGTQYWINGDPANFPNERLYVQKTSCMEGACTAVQYADYGYVDEANPVAYTKTTWQY
                     NATEGSITGQTLAESASMSYENYLAEVNAMRAGAQEYTGWSLNNKNFQGVFAGFNSDS
                     NNLWYNMANGQPTSIELAGLYSDAKGSQAPGIMTTGLASFNPFNAMDPLVNSESPIGG
                     AYYAYMGAAFGKHQVASDLFDGLISGLYLNPDGKAGILYGSIGGYNQPDTGYFHGSGA
                     INGGCQLGEGFNGLSAQNFAYNVIQSSQQWNYGTNDVTVIGDNVKLTTINRNNAWINQ
                     NYLSDNGFWGVVQTVRGGTKDSGLAPTAWAWAAHQMNASTPRSDYTGIEITKTENGIS
                     TGNYVGAAAIYSGGPVSDPSFTAVSAGSIKGLFDPTATTWQAVSQGSFMETKNFLAKA
                     AAMSGDAAAQEAFMNATKIPAIQVGQATLSQAPNTVVNNLSNVTMNNVAFFAYSTGAV
                     PKIWATNDVRGNYEVIGGYSVAPSTSGAAINNVPLSGNGLTATFNVNHWNGSTWGANV
                     AGSGNLSGGSYAGPVQFQGGAAGTIQSGSFSGTGAGIAK"
BASE COUNT         5004 a         6428 c         6567 g         5464 t
ORIGIN      
        1 agagctgctc tacaagcatg acaaggtgcg cgacgcctgc gtcatcggcg tgcccgacga
       61 agcccaggtg caggcggtga aggcgttcgt cgtattgaaa aaccccgccg acgcgggtcc
      121 ggcagcggaa aaggaactca tccactattg ccgcgagaat ctgatcaagt ggagctgtcc
      181 gcgttccatc gaattccgca ctgatctgcc caaaaccctg gtcggcaaaa tcgccttcaa
      241 cacgctggag aaggaggaag tcgaaaagct gaaaaaggaa ggcaaatttg ccggatgaat
      301 ggagggcaaa ttttcattct ttccctttcc aacggaacaa tccttcaacc ccgggaaggc
      361 aagggaatgg ttccatttgt tttgtgccgc tccatcaaga tggaactttc ccttgatttt
      421 ccgccggcgg ataagatccg tcttcaagat gcacttccgc gccggtgacg ggcgggttga
      481 aaacgcacag cagacgcatg tctttggacg cccgcaaaag atgctcgtcg tggttgttca
      541 gcgcgtatat tgttcccggc acgatgtggt gaatttttcc gtcggccggc gtttccacct
      601 cgccttcgcc ttcaacgcag aaaacggctt ccagatgatt cttgtaccag atgtgcgttt
      661 gtgttcccgc gaagatgatg gtttcatgaa acgaaaaacc cattccgtct tttttcaaca
      721 gaagccggcg gctgacccag tttttatttt cagcccagac ctcgcgatct gttcccgtga
      781 tatcattgat cgttcgaacc agcatgcctt gtcctttcct ttaacggttt ttcaacacgc
      841 tgccgatgga cgcatccaga atatccaggc cctccatgag cacctcatcg ggaataatca
      901 gcggggcaag gaatttgatg acattgtttc tggcgccggc caattccatg atcagtttgt
      961 tcttgaagca ttccatggaa atttctctgg cgatttcggg cgggtcgacg gccatcccgt
     1021 aaatgaatcc tcgcccccgt acttccgcgt tcaactgagg atatctggcg cagatggcat
     1081 ggaggcggtc ggaaaggatt gtccccttgc ggaaaatatc tttggagaaa cggtcgtcct
     1141 tccagtagtc cagcgccacg gaagcggaaa caaaggcgag attgtttccg cggaaggttc
     1201 cggtatgctg accggctttc cagatgtcaa gttcccggcg gaacaaaacc agcgacatcg
     1261 gcaggccgta gccggaaaag gatttggaaa ccgtgacgat atccggtttg atgccggagc
     1321 gttcaaaact gaaaaaatgt ccggtgcggc cgttgccgac ctgaatgtca tcaaccatga
     1381 gcaggatgtc gaactcgcgg cagatggatt caacttcgcg cagccattcg tcggatgcca
     1441 cgcggattcc gccctcgccc tgaacggttt caagaatgac ggccgacgga atatcgacgc
     1501 cgctgctgtt atcttcaaga aagcgtcgca tatactgggc cgtattgacg tcttttccca
     1561 gatagccgtc gtagggcaga aaggaaacgt ccgaacggtt gatgaaggcc tcgtcgcggt
     1621 agtaggcatt ggccgtgacc gacatcgatc ccatcgtgag accgtgatag ccgttggtaa
     1681 atgaaatcac gtttgaccgt tttttcacca tccgcgcgag tttaagcccc gcttccacgg
     1741 cgttggtgcc ggtgggaccg gtgaattgga ttttgtaatc gaggttgcgc ggtttgaaca
     1801 caatgctctc cattttaaga agcagctctt ccttggcaac ggtggccatg tccagaccat
     1861 gaacgatccc gtccgtttcc aaatactcga tgagcttctt tttcatgtgg tcattgttgt
     1921 gcccgtaatt gagcgtgccc gctcccgcaa aaaagtcaat gtagcggttg ccgtccgcgt
     1981 cgaataaaaa agcgtctttt gccttttcaa acaagacggg gaacgaccgg gaatagctgc
     2041 ggacttcaga ctccaaacgt ctaatggttt tcatcagatt tcctcctttg atcacaggtc
     2101 aaacggcccg attcggaaca agacttcctc ttcatgacgc tgatctccga aatcatcggc
     2161 ggaaaacaga acgctttgca ccagcgggac gccaagcgaa gcggccagtg aagaaaaaag
     2221 ggcctttgac ggaatattgg acggactgac cgtcgtttcc aggtaacgcg ggggacaggg
     2281 ggtgggccgg ttcaaaatgg aagacagcat ggagcgggcg acgcctttcc cccgatactt
     2341 tttatccacc gcgacctgcc agacaaacaa agtgtcctcc tcatcgggac ggaggtaggc
     2401 ggaaataaag ccgcaaacgg cgtctgcatc gcaggcgacg acggaagtcc gggaaaaatg
     2461 cgcgcacagg ataagataac aataaagcga attgagatcc agcggcggag aatctttaac
     2521 aagcttttca atgaacggcg catcagcaac ttccggccgg cgaaaataca tatccgttgg
     2581 atcttttttc aagtccatct cctgaacatt gattgtttat gttgtgccgt tatgagaacg
     2641 acgggagcct ctgggtgatc aaaaaatgac aagtgtcttt tttgaatgat tctttggtag
     2701 cacaaaacca atgaaaatgc aacctggtgg gacaaccgtt ccattcggct atattgcggt
     2761 tccattgaaa tatggccgaa aatgctttgt tccgtggaat gttttatagg ttgctaaatc
     2821 agggaaattg catgtaaagg aagattgcgg caaagagaat tacatttggt ctgcgattcg
     2881 ggaattcgat gaagaaatta ttttataaaa tttcgatcta tctgtcccgc cgtctggggc
     2941 tgtggttttt ccgtgtgttt tcctggttta tcgcaacggg gtactttttc ttgtttccgc
     3001 cccgcgtccg ggacagcctt cagttttacc gggcgctttt cccgggacga agctttggat
     3061 atcacctcta ctgcgtctgg aagcagttcc acaatttcac gggcgtctat gttcaccgct
     3121 tcattccctg ggcggccgag gaggcgaagt tcacccgcga aggctgggag catctggatg
     3181 aggccgtggc caaaaaaacc ggcgcgatcg tggtgatgtc gcacatcgga aactggtaga
     3241 tgctattgta gccgataaat ccacgctcgc accgttgaat gtctggcgag cctatgaact
     3301 tattggatgc ttcgacaagc tcaggatccg aatcgttgac tgagcaaaat gcgcaaccgc
     3361 agcccctgaa aaccattgcc gatccggcct gcgggaccgg cggctttttt ctggcggttt
     3421 atgattgcat ttccgatccg caaaacttta tcctgaacaa agaacaaaaa ttattcttaa
     3481 agaattcaac gtattacggc aacgagatcg tcgcggcagc gcgccggctg gcgctcatga
     3541 acatgtttct gcacaatatc ggcgatattg atgccgagac gttcatcctg cctactgatt
     3601 cactggttgc cgatacgggt ttgcgtgtgg attatgtgct gaccaatccg cccttcggga
     3661 agaaaagttc catgaccttc accaatgaag aaggcgaaca ggagaaagac gatcttactt
     3721 acaaccggca ggatttctgg gcgacaacca gcaacaagca actcaatttt gtccagcaca
     3781 ttcgcacaat gctgaaaacc acaggcaaag cttcggttgt tgtgccggat aatgttttgt
     3841 tcgagggcgg cgcgggtgaa atcgttcgca aaaaattgct ggaaacaacc gatctgcata
     3901 caatcctgtg aagtatgatg accttcagga ttttattgaa tgctacaagc cgtccaaccg
     3961 ccataaacgc aaggaaacgt ggcatccgga aaaaacccag aaggccgccg gcgcaaattt
     4021 acctatgatg aaattgtcgc ccgcgataaa accagcctgg acattgcctg gctaaaagac
     4081 aaaagccttg ccgatctcaa caatctgccc cacccggatg cgctggccaa cgatatcata
     4141 gagaaccttg agtcggcagt tgcaagtttt aaagaaatca tggaaacgat taacggtaaa
     4201 tagttcaacc ggtcgaaaaa acagatttgc agcccgtgaa ggctattgcc tcgaaggcga
     4261 aggatagccc gtaatatctt gaattttttc catgagcggc cttaagcggc cgtacatggc
     4321 gagatacacc tcccggtaaa gacggtcgta gatccggtga ttttcggcaa cgggctcaaa
     4381 tacttccttt acccgcgtca tggccttgac cgccgacggg aaatccggat aaagcccaag
     4441 tcccacagcc gcgtcaatgg cggcgcccag ggccgaagtt tcataggtat gagggcgctc
     4501 cgcgggcagg ccgaagatat ccgccgcaat gcgcatggcc atatcgcttt gcgatcctcc
     4561 gccggaaacc cgaaggcggg aaacggccgt tcccgttcgc ttttgagtga gttccgcgcc
     4621 ttctctcagt ccgtacacca gcccctcgag aatggcccgg tacagataag cccggttgtg
     4681 cacatccccg aaaccgataa tcgatccttt ggcataggga tccgtattgg ttcccggcga
     4741 ccagtagggc tgaagaatca ggcccatcga accgggcgga actttttcaa tcaactgatc
     4801 aaaaagcttt tccggagcga tattgttttt ttcggctatc ttccgctcca gcattccgaa
     4861 ttcatcgcga aaccagctca ccatccagaa accgcgatag atcatcactt ccgtgtaata
     4921 ggtatccggc acggaggcgg ggtagggcgg aatcatggac agcagttcga tgtatttcgt
     4981 cgtcgtcgta ttgaaagtgg cggtcgttcc aaaactcaaa cagccggtgt ccggcgtcag
     5041 acagcccgcg cccagcatct cgcatccctt gtccgttcct gcggcaatga cgggcagccc
     5101 cgcgggaatt ccggtttcct ctccggcccg ggcggtgatt ctccccagaa cctcgcaggg
     5161 tttgaccagg cggggcagct tctccttttc gatgggaaaa agccgccatt tcaaatcgaa
     5221 tctgcccgcc cattgatacg ttttgttgtc gaagggaaga tagccgacat tgcaggccgc
     5281 cgaatccgca tattcccccg tcagcataaa ggtgaaaaat ccggacagaa gaaggtattt
     5341 atgggtttgc cgccagattt ccggctgatg ctgctgtatc cagtttgacg gacagctctg
     5401 gaccgcatta ttgagacggt cccataagcc tatcgatttc agaagcggtt tggcaaagga
     5461 gggaacgatc tcttcggcgt ttgcttttct ctggtcaagc cacagacagg cgggccgcag
     5521 aggccgtccg tcttctccca gattgatcat ggtggcgcgc tgcgtggtca gggtcacggc
     5581 gacaatcgcc tctctggatg atttgtccga cttgtccagc agctcccggc aggcctcgca
     5641 aaacattttc cagtaataat ccgcatgctg ctccgcccag cccggctcag gcgaaaaata
     5701 cggttcaatc cggcgtttga cgatctgcgc gatatttccg gaagcgtcaa tcagggccgc
     5761 ccgaatggac tgggttcccg cgtcaaacga caaaatcatt tctccgcttg gtttcttcac
     5821 gttttccatc gtttttcccc tgatgatttc aattccaaaa gtccgcccgg attcataatg
     5881 ccgcgcgggt caaaatgatt cttcagcgcc cgcagaaccg ccatctgctc ttttcccaga
     5941 tgcgtttcca tccagggagc catcatgcgc ccgatgccgt gatgatggct caaggatcct
     6001 ccgtatttct gaatgctgtc gataatgccc cgctggaatt tccggaattc cccgggatcg
     6061 ttcatccgga tgatgaagat gaaataaaga ttggttccct gagggtaaaa gtgcgaggcg
     6121 tgcgtcatgc acatggtgtc cggccggctc ttgatatagg cgcgcactcc ctgatgcagg
     6181 cggtgaagat tgtcccaggt taccgccgcc tccagcgtgt cgatcagaat gccgtaatcg
     6241 cccagatctt cgcgcatgta aggttcggtg tagcggctct gctcccattt gcgcgcggcg
     6301 tagccgctaa gcgatacggc gccgtggcgc ctggctgtcg acttgatctt ttttgccacc
     6361 aggcgcgaat aatctttgtc tccttcgcag ttgcccaggc aaaggcagcg gcaaagcggc
     6421 ttaaaacccc ggagggtcag aaaccgatcc gccagatccg gaatgccgta gagcttcaat
     6481 ccccggtcgg tttcttcggg atcggaaatg cgatagacgg ccggacggcc gaattccgac
     6541 tgcatgattt cgcggctggc ttcgacggct ttttcccatg aagggtacat aaaactgaag
     6601 tacgttctgt tttccggcag gtaacggaaa attttcatgg tcacttccac cagaatgccg
     6661 aaagcgccct cggaaccctt cataatgtcg tttaccttgg ggccggtggc cgttgccgga
     6721 taatccagcg ttttgaatgt gccggccgga gtgacgtatt cctggctgaa cacaatgtcg
     6781 caggcgtcgc cgtagtaagt ggaagcctgt ccggagccca gtgtcgccac ccagccgccc
     6841 actgtcgaat attcaaagga ttgcggaaaa tggccgcagg tgtagcgatg cttcgtctgc
     6901 cagagttgag gcgcgcggtt aagggcgtcc tcgcaggccg gtccgaacat gcccggctgc
     6961 atacgcgctg tctggtttat ttcgttgatt tcaagtagtt tgttcatgtg cgtgctcatc
     7021 gccagcgaca cgccgccccg ggacgggcgg cagccgaaat tgacggagga gccgccgctg
     7081 aaaaccgtca ccggaatcag ctcccgatcg cagtattcga caattttgcg cacatcgtgc
     7141 ttgtcgcgcg gatgcaccac cacatccgcc acctcccgga caatgccttc ccgaagctcc
     7201 agcatctctt ccgtggtttt tcccgaggaa tattttaagc ggctgtaatc gtcgcaggcg
     7261 acgttttcca cgcccgcgat ggcgcgcaat gcttcgattt ggcccggctt cagcgcggac
     7321 ggccggttga gtgaaaccag attgaggcct tcgccggatt tggcggaaaa atcctcgtcg
     7381 gtgagatgaa aatcctgctt gagcattgca taccaggcat cgctggggtg cttgaagtgg
     7441 tttggatttc catatttaaa gatcgatcgg taggttcccg cgggcggtgc tttttccgtc
     7501 cagtccggtc ggaaattcgt ggctgtcatc cggctgtcct ccctgtaatc cggtcttgtt
     7561 attgcggcag ccggagcgtg ttgatggtcc cggcaatttc ccgttccatt ctttccggcg
     7621 tccattgcag ttctttggcc gccaccaccg ccaccttgcg caaaatcttc tcgccgggat
     7681 tgcccagcgt ggcgattccc gtgcgcctga gcactatatc ggaaagagtg cgcgccattt
     7741 cctcgcgcac ggcataaacc acctcggcaa aaatctcgcc gtcttcgttg atttgttcag
     7801 ccagctcctt gttttcatgg gctagtttgg taattgcccg gtagtccgtg ccgtagcagc
     7861 gccccaggta atccagtgtg tttcggcaaa agccgtttgt gttgtgctgg tgaatttctt
     7921 ccatgaagaa attcaaattt ttgatccggc agcccgcaag atgatgccgg gctgtcttgc
     7981 ttttcgccct gggcctgccc agtttggcct cgacaagatc cacgcatttt tcggccaggt
     8041 tgcggctggt ggtgaatttt ccgccctcag cggtaatcag tccgttgagt ccgttttcct
     8101 tattgtccaa tatctcataa cgccttgaac tggtataggt ttcttcggtt tgtccttcca
     8161 ccagcgggcg cagaccgcca taggtgtgca caacatccgc gtaagaaagc gttccgtcgc
     8221 cgaatgaccc gttgacttcg tcaatcaatt ccagaatgcc ggccttggtg acatggtagg
     8281 cgtccggatt gccgacaaac ggcttatccg ttgtgccgat gatcgtatgg ccgcgccagg
     8341 gaatgaggaa aaaatggcgg cctttgggcg tcatgcaggc caccacgtat ttgccggaaa
     8401 ccagcttctt tttcgtgatg atgtgaattc cctcggaacg ccgcagcgcc gcgccgccgg
     8461 ccgaattcga ttcggccaga cccagcagaa gatcagccca gggaccggcg cagttgatcg
     8521 ttacggatga ggaaatggcc atggttttgc cggttaaaag gtctttcacc cgcacgccgg
     8581 cgacgttttt ttgcgcatcg gttaaaaagc tttccacctt tgcataattg gccaccttgg
     8641 ccccgcaggc cgccgccgac ttgataaagg ccagggtcag ccgctccgga aaaatgctca
     8701 tgcagtcata aaacagcgat gcccccgtca aaccggcggg gcgcacgttt ttttcacttt
     8761 ccagaaccgt ccgtctggaa atcgtcttgt gccgcgggat ttttttgctg gcatcccagg
     8821 tatatttttt gtcgtaggaa agcgcgtcat acatcagcat gccggctttt accacccatt
     8881 tgctgttttt gaggggcttt ttataatgcg tcatcaggat ggggatcgga tagacaaaat
     8941 tgggggcgat gttttccagc acgcggcgtt cgcgcaagga ttcgcgcacc agaccgattt
     9001 cgccgttggc cagataccgc aaaccgccat ggatcaattt ggaggtgacc gatgacgtgg
     9061 cccaggaaaa atctttcttt tccaaaagcg ccactttcaa tccgcgcgcg gcggcttcat
     9121 aagcaaccga tgcgccggta attcctccgc ccacgaccag cacgtcaaat ttttcgtttg
     9181 tgtaattctc tataaatctt aacattttca ggattccttc tgctttttgt ttccggacaa
     9241 gcccgctttg acgacctctt cggcgtaaat cattatttct ctcatgattt tgcgcgcttc
     9301 tatcggcttt tggcttttga tggcttcata aatatcccgg tgaaacttcc gggaacgctg
     9361 tgcgtttttt tcatcgttaa aatagagagg tccgtaatcg cgaaagatct ggttgaagaa
     9421 attcagcccg atggtgcaaa gcagattatg cgtgctgcgg gcgatgatct ggtgcacttt
     9481 gatatcccgt tcgagcatgg tcagctcctc gctcccggtc acccgctcca gttcaagaag
     9541 atcctcattc gaacgccttg cggcggcgag ccaggctatc tgcggcatca ggtaacgccg
     9601 cgcttcgaga atatccagca ccacttcttc gcgcccgtcc agattggcgg cggctctgat
     9661 cagatccaga ttgccgctgt ccagataatt tttgacatac aggccgttgc cgtgccggat
     9721 ttccacaagt tccagaatct ccagcttccg cagggcctcg cgcagcgtgg cgcggttcac
     9781 ctgaaaggtt tccgccagct cccgctccgg cggtattttg tctcccggag caagcttgcc
     9841 cgacaaaatc tgattttgta aaacggtgac aatctcttca ttcaggcgcg cacgggaaac
     9901 cggctgcgcc aaaacctttt ttgccgtcat taactttttc ctttcctgca ccagcccgta
     9961 taattggtct gatggttgaa ccaatatacc aattgcaaag gatagtcaac aggtgttttt
    10021 gttattgaaa tatattcaat aatttgaaat atttacaacg cattcgcaaa cccagccaaa
    10081 acgacgggtt gcatttaaag ggcgcctgtg gcaaaacaat gacgttgctt tacgatttgg
    10141 aaatccgatg aagaaattat tttataaaat ttcgatttat ctgtcccgcc gtctggggct
    10201 gtggtttttc cgtgtgtttt cctggtttat cgcaacgggg tactttttct tgtttccgcc
    10261 ccgcgtccgg gacagccttc agttttaccg ggcgcttttc ccgggacgaa gctttggata
    10321 tcacctctac tgcgtctgga agcagttcca caactttacg ggcgtctatg tccaccgctt
    10381 cattccctgg gcggccgagg aggcgaagtt cacccgagaa ggctgggagc atctggatga
    10441 ggccgtagcc aaaaaaaccg gtgcgatcgt ggtgatgtcg catatcggaa actgggagct
    10501 tgccgctcag aggttgcagg aaaagggcct gccggtcatg ctttaccttg gggccaagca
    10561 caaggagcag atcgaaaaga tgcaaaagga aaaactcgcc caaaccggaa ttcgcatcgt
    10621 caccacggac gaaaaggaaa agtcgccttt tgcgcttctg gagggtgtcg gcttccttcg
    10681 tgaaggcggc atcgtctcga tggcgggcga caggctctgg ggcgaccaga cctcggtgga
    10741 agttgatttt ctggagcaca aagtccgtct gccggatacg ccgcatcttt ttgcgctgat
    10801 gagcggtgcg ccgctgatga cgttctttgt ccatgaaaaa gcgccgggcc actaccacgt
    10861 gcaggtttca aaaggccgga aggtggcggc ggccgcccgc gccgaaagaa aaaaagcggt
    10921 gcaggaatcc gtgcaggcct atgcggatga tctggcgcgc ttcgccgccg ctcatccttt
    10981 tgaatggcat catttcgagc cgtttctcgg tgaaaagatc atcccgtaga ggagcgccgc
    11041 cgggcagaat ttgctttgcc cacgcggcaa attagtatag aaaggcattg aaatctttaa
    11101 aagaggacgt gaaatgaacg ttcgggaagt gccgcaggac atcacttatt acgagggaaa
    11161 aaagcgagcc tgctgggcgc tcaatgacga ggggaaatat gtcattgtgc ccagctccgg
    11221 ctgggaggtg gaagcggtgg tcaacggcct ggccgttgaa gaaatggcgg caagtctgga
    11281 ggcgacgagg caggcggtgc tggcgggcgt caaaagtccg ctttgctatt acatggagct
    11341 tcgccagatg acgccggaga ttctgggaaa aacggcgcgc gtcgcggctt ttcgggtcag
    11401 acggcatttc cgtcccgacg tgttttccaa actgggtccg tcggttctgg accgatacgc
    11461 cagggcgctg gccgttaccc tagatgagct caaaaccgtt ccgcaaacct gattctggag
    11521 aggcagcctg catgaccacg gaagtcaccg cagccgggaa accctttgtc cacgttcatt
    11581 ccgcgcactg tgaaagcggc gtcaccgccg cgctgtttcg ggacaaaggc gtggaaatct
    11641 ccgagccgat gattttcggc atcgggagcg gcatcttttt cggccatctg ccgtttgtga
    11701 aaatgggcgg cgtgacgatc agcacgtttc gctcccagcc gggggccgtt ttccgtaaat
    11761 ccgccaagcg cctcggcggc aaattcaaaa ccgcgcggtt tcgcaacccg aagaagggaa
    11821 tggacgaact gaggcgcgtc ctcggcaccg gccagatcgt cgggctgacc accaatatct
    11881 actggctttc ctattttccc aaccgtttcc gctttcagtt caacgggcat aatatcgtcg
    11941 ttctgcatga gatcgagaac ggttttcgcg tgagcgaccc gatgctggag gagccggtgg
    12001 attgtccggc ggaagacctg gaaagggcga ggtttgcgcg tggtccgctc gaatcgagag
    12061 ggttcatgta ctatccacaa tccgtcaacc ccaaccccga cctgcgcgcg gccggccttc
    12121 agggcatgaa agacacctgc aaaatgatgc tgaaaatccc cctgcccatt ttcggcattc
    12181 ggggaatgcg ctatctggct aaaaaaatca ttcgcagccc cgagaagctg ggcttcatgg
    12241 gggcctgccg cgagttgtcc agcgtggtcc gaatgcagga ggaagtgggc acgggcggcg
    12301 cagggttccg ctacatgtac gcggcgtttc ttcaggaagc cgccgagctt ttcgcatcga
    12361 aggagcttgc ccgtttatcc ggcgaaatga cggccatcgg cgacatctgg agggaatttg
    12421 ccgtcgtctc cgcgcgcatc attaaaaagc gcggacagac ggaagagacg tttgaaaaag
    12481 cggggggcct gatgctgatc tgcgccgggc gcgaagaaca gctcttcaag gatctggcta
    12541 aggcggcagg gaagctcaag gcatgaacgc gctcgacatt tcttctgtga ccttttgcta
    12601 tgacggatcg cccgctccgg cgcttcggga tttttccatg gccgtcgcgg acgggcggac
    12661 gcacgcgctt ctggggccaa acggcgccgg gaagtccacg ctgatgcgga tcatcaccgg
    12721 ccagttacag ggccaaaccg gcggcgtgtc cgtgtacggc cttcgcatgc ccgactcaaa
    12781 agcgctttcg ttcatcgggt gcgcgccgca gccgatatct ctttacccgt ctttgaccgc
    12841 gcgggagaat ctgcgctttt tcggcgcgat ggcgggaatg tcaaaggatc agatcgcttc
    12901 gcgctcggat tgggcgctcg aaaaaacggg cctcgcggag tcttccgaag cggccgtggc
    12961 gtctttttcc ggcggcatgc aaaggcgact caacctggcg gccgcgcttc ttcattcccc
    13021 gaagcttctg ctgttggatg aaccatcggc gggcgtcgat ccgcaaagcc gaaaccatat
    13081 gtacgacctg ctcatggagc taaacgcggg tggcgtcacg attctgcttt gcacgcacat
    13141 gatggaagag gcccagcgcc tgtgctccgg cctgacgctt ctggaccggg gcgaacggat
    13201 ttatgacggc cgaatgtccg atatcggcaa cctggaagaa ttttttctga agaagacggg
    13261 cagggggctt cgtgatcttt aaacaactaa tagaactggt ccgcaaggaa atgctggtcc
    13321 tcgttcgcga ccggcagacc atcctgcttc ttttcctgat gccggtggct ctgattttct
    13381 tcatgtcgct ggccctggaa ggcgtctggg cggacaaact ggtcgggcga aagatttcga
    13441 tggtggtgga caaccagagc cggtcttacc aggccaacct tcttgcggga aaaatcaagg
    13501 cgcatgcgct gatcgtgccg gtggaaaggc cggcgggagc ggatgttgat cgaatttttg
    13561 ccgaaggcac agcgcaggcc gtcgtgacga ttccccccgg tttcgaagaa ggagaccggc
    13621 cggtggaaat tttctttgat cccgtgatgg acgcaagcta caggattgcc gcccgctcgc
    13681 tgatcacgtc gcttgtcgtg gaagtggtca tgggcgtcga ccatctggag gcggtggtgg
    13741 cgggcctggt cgtcgagcgg agcggaccca accgggagct gccgagtccc ttgcagcaga
    13801 cggtcccggc ctatgccatc ttcgcgatgt tctttatcgc cattcccatg tccgtcggtt
    13861 tcctgagaga aaagaaagat gggacgctcc agcgtctctt cacgtacccg gtgtccgcga
    13921 atctggtgac gctgggcaaa attactccct acttcctgat caacgttgtt cagtttgttc
    13981 tgatgatgct ggtgggggtt tttgtcatgt cccgctttat taccccggcg tttttcgcag
    14041 ggcagcatcc ctggcacatg ctaccggtga cgctggtggt cgcggccgcc acgacggggt
    14101 tcggcgtact ggtggcggcg acggccaaaa cgcccgagca gtcttccacg ctggctgcga
    14161 cgggagcgat tttgatgggc gttttcggcg ggatcatggt gccgcacgca gtgatgccgc
    14221 cggtgatgaa aaagctggcc atgatttcgc cgatgttctg ggcgcatcag gcttatctgg
    14281 atatttttct gcgggatgcc gcgtttggga tcattctgcc caggttaatc atcttgaccc
    14341 tgtttgcggg aatttgcttt tatattgcgg ggaggaaggt tcgatggatt tagaacaggc
    14401 gtacagggat ctgaacgtga cgtcgcggga ggatctcctt ttcaagctca aggaactggt
    14461 catccgggcc tgcgaaatcc gggatgtccg gacggaggac gtgcccaccg acgtgccgtt
    14521 tatcggcgga cccgggccgc tcaagctcga ctcgctcgac gcgatggaaa tcgcgatgga
    14581 actgcgctac acgttcggcg tggagctgaa aaacgcgtcc acggccgcga aggcgatgca
    14641 gaacttcgat acgctggctg attttgtgat cgccgcgccg aaggtgaaaa agtgagaaac
    14701 ggcaatatcc atattcgcgg atggggcgcc gtcacggcgc tgggctgggg cgcggaccag
    14761 ggcgcccgcc gcctgatcgc gggcgatgtc catcccgcgg gcgtctgcgg gtctgcccac
    14821 ctggtcaacc gggagttcaa gggatttgaa gtcgcctgtg acggcaatcc cctggaaaag
    14881 gcgatgaaaa tgctggatgc gtcgatagcc gaggcctttg agcgaggagg cctttccgcg
    14941 cccgaaattg ccgagtccgc tctgctcgtg ggcaccaccg gcggtatctt cattcgcaac
    15001 gagcatgagt ttaccgaatc cgtgcgccgg aatcccggca aggaatcgcc gccgattgtc
    15061 tgccgcaacc gggggccggg cgaagtggcc gacgtgatcg cccgaagata cggcattcgc
    15121 ggcctgccca tgaccttcag catggcctgc gtttcgagtt cgcacgcgct tgtcgtggcg
    15181 gcggatctga tcgcgcagaa aagaatcaga cgcgccgtcg tggtaggttt tgaaccgctt
    15241 ttgaatctga ccctgcaggg ttttgcaagc ctgctcctgt atgatccgga cgagtgccgc
    15301 cccttctgca accgtcgcat gggcatgcag atcggcgagg cggcctcctg cgtgatcctg
    15361 gaagaaagcg atagcgccgc cgcgggcaaa agcggctttg catttgccgg cggccatctg
    15421 tataacgaca acaaaagcct gacctccgcc ggcacggacg gcgaaatggt gaggggcgtc
    15481 gtttcccggg cgcttttgaa agccggaacg gagccagggg aaattgtcgc cgtcaaagcg
    15541 cacggtacgg gcacgagaga caatgatctt tccgagggaa gggggctggc gaaggtcttt
    15601 gagaagcatc tgccgcccgt cgtctcactc aaggccacca tcggtcacac gctgggtgcg
    15661 gccgggacgg tcgagacggc tctgtggctt tcctgcctgg aggcaggtgt gattccgaag
    15721 agcttcggct ttgccgaaac ggaccccgaa atcggttttg cgccgtcgca ggaaaatctt
    15781 ccggcagccg ccggggcgta tctgtttacc tatttcggct tcggcggaac ctgcacggct
    15841 tatgttatca ggaaaatggc atcttaaaat atgactgaaa aattaaaaca ttcttccctg
    15901 cccgtcatcg cggccggcta tcttgggccc gcgctggttc ccaatgcgga cggcgcgtgg
    15961 gaggaggcga aactgccggc gaatcccgac gatctggtgc agaaaatgat ggggcagccc
    16021 atgcggcgcg cgggacggtt catcaagctg gtctgctgcg gcgcgctggc ctgcatgtca
    16081 cgcgttcccg aggaaacgct gcgggggcgg agcatcggca tttttctagc caccggtctg
    16141 ggcaacatcg atgaaatcct gccgttcatc acgcaggtat tcaaacacga cggcgggttt
    16201 cccagcccca accagtttgc caattccgtc agcaacgccg ccgccttttt cctggcgaag
    16261 gcctgtggga tcaagggcgt tgttctcacc gtctcgcagg aagagctttc ttttgaatcc
    16321 gccctgtggc tggcgcaggg ctacctggcg tcaggcgaga tcgacctggc gctggtcgga
    16381 ggctgcgacg tcttcacgcc gtccgtggaa gactaccggg agcggttgaa cctggttccc
    16441 ggccgctgtc ggcgggctcc cctgggcgaa ggcagtgcct ggtttttgct gggtggtgac
    16501 ggagaagcga aaataggaga tattctgtct gttgaaattt cgagcgggtt agcggacgca
    16561 gaaagtctgc catcagacga tctcctgaaa caatgtgaaa aattgtttga agaatttcgg
    16621 aacaacatgg aatccccgga caatggcgcc aactcagcct tgttgtcatt cccgcgaaag
    16681 cgggaatcca ggccctctga tggtctggta gattcccgcc tacgcgggaa tgacagagaa
    16741 gaagggtttt tcgaagacga taaaatcagc aaagtgttcc tgcccggatt tcgccttgcg
    16801 aacctcgacg aagcgtcgtg gaaaaagagc ggctgggaca tggatgatta tttgatccgc
    16861 tgcggcattt cctccaccgc ttccgcctgc ggactggcgc gggcgctgct ttcccgccaa
    16921 agctcgtttc tggttcatgg aaatttgtcc tcgtccggaa ggccggcgct gatgtcagcg
    16981 gtaacaaaat agccagtcat tcctacgata gtaggaccaa ggcaaagaag caagcgcacc
    17041 taataacctg aaggtcacgc gaggtacgca ctacataaaa aaatgtaggg cgggtcttta
    17101 gacccgctaa taacctcggc gcactgatta ttttcatgcc cgtgtggcgt cgcgagggac
    17161 aaataaatca tggtgttacg tcaacaaaac aaacgcatcg ttctggttca tccccgcggg
    17221 tccaactggt ttcccggcag gcgcgacatc accgacattg ccaaccgtat ggtgccgcag
    17281 ggaatgctct ccatcgcggc ctggctgacg cgcgatgggc acgatgtttt tgtctatgac
    17341 tgcctggggc ccggagcgcc ggtggacacg aaccggcagg ctcacgatat tctggcgttc
    17401 gacccgcaaa tagtcggttt ttccgccacc acgtcgtcgt tccccgacgc ggccgacctt
    17461 gcgcaaaaaa ttaaagacag ccggcccgac actgtcacgg tctgcggcgg cgtgcatgta
    17521 tcggccctgc gcgaaattct tctggcggct tatcccgctt tcgattatct ggcggcgggt
    17581 gaaggcgaac tgaccctggg ggaactggca gcaggtgttg atccctcgga aattgcggga
    17641 ctgatccgcc gggaaaacgg cattgccgtc gccaacgaac cgagaaagca tattacgaac
    17701 ctcgatgatc tgccgtttcc cgcctatgaa aaactccgcg ggtttccccg cgactaccac
    17761 ctgccgcttt tcagctacaa acaaaccccc ggcgcgacga tgatcacttc gcgcggctgc
    17821 atgtattcct gctcctactg cgaccgctcc gtttttcaaa agggtttccg ctacaactcg
    17881 gcggcctata tttacgacca catgaaatac ctgcgcgaaa aattcggcgc ccgccacgtc
    17941 aatatttacg acgatctctt cactgccaac cgcaagcgga ttgtggaact ctgcgaaaag
    18001 cttgcgcgac agccgctggg catgaacttc aactgcgccg tgcgcgtggg ctacacggat
    18061 gatgatctgc tcggaatgct caaagaggcg ggctgcctga tggtgtcctt gggaatcgaa
    18121 tcggccgatc cggacatgtt ggcccgccac aagtccggcg tatcgcttga cgatgtgcgc
    18181 gaaaccgtaa gccggattca gtcggccgga ctgcgcgcca aagggctttt catgatgggg
    18241 ctcccggggg agaccgaagc gtcgattcga cgcacgtcgg atttcattat ttcgctgggg
    18301 ctcgatgaca tgaacatggc caagttcacg ccctttcccg gcgcgccgct gtggggggcg
    18361 atccgtgagg aaggaacctt cacggaggat tggcggctga tgaactgcct gaattttgtt
    18421 tttattccgc gcggaatcga atccaaggaa aaactcgatc acctgtacaa cgagcacgtc
    18481 aaacgctttt attccgatcc ggcctggcgc aggcggttcc ggggccgcat ctgggagcac
    18541 cgccacagca tgctgtatct gcttaggcat cttccctcct tctggtcggc caaaaaccag
    18601 tttgaaccag actagagcct ttttctgtgt catggtgagc ctgtcgaacc gtgacccttc
    18661 gaactagaag gtaatgaacg aaaaaagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18781 nnnnnnntgg tgagcctgtc gaaccgtgac ccttcgaact agaaggtaat gaacgaaaaa
    18841 agggaggcgc gtgaatcacg cgcctccctt tttggttgat gtttaaataa atttacaaag
    18901 tcatgccttt ttcatacacg ccgctttatt tggctatccc cgcgcctgtg ccggagaatg
    18961 atccagattg aatagttccc gctgcgcctc cctggaactg aaccggtccc gcataggaac
    19021 cgccgctcag atttccgctt cccgcaacat tggcgcccca ggtgctgccg ttccagtgat
    19081 tgacgttgaa ggttgcggta aggccgttgc cgctcaatgg cacgttgttt atggccgcgc
    19141 cgctggttga cggcgcgacc gaataaccgc cgattacttc ataattgcct cttacgtcgt
    19201 tggtcgccca gattttcggg acggcgcccg tcgaataggc aaagaaggcc acattgttca
    19261 tggttacatt gctcaggttg tttaccacgg tgttgggggc ctggctcaaa gttgcctgac
    19321 ccacctgaat tgccggaatc tttgtggcgt tcatgaacgc ctcctgggct gccgcgtcac
    19381 cgctcatggc ggcggctttt gccaggaagt tcttcgtttc catgaagctg ccctgggata
    19441 ccgcctgcca ggtggtggcg gtcgggtcga agagcccctt gatgcttccg gcgctgacgg
    19501 ccgtaaagct cggatcggag actggaccgc cggaataaat cgcggccgcg cccacgtagt
    19561 tgccggtgct gatgccgttt tccgttttgg tgatttcaat gcccgtatag tctgatcgcg
    19621 gtgtagaggc attcatctgg tgagccgccc atgcccaggc ggtcggagca agacccgaat
    19681 ccttggtccc cccccttacg gtttggacga cgccccagaa gccgttgtcg gagagataat
    19741 tctgattaat ccaggcgttg tttctgttga tggtcgtcag ctttacgttg tctccaatta
    19801 cggttacgtc gtttgttccg taattccact gttgggaaga ttggattaca ttgtacgcaa
    19861 agttctgagc gctcaggccg ttgaatcctt cgccaagctg gcagcctccg ttgatggcgc
    19921 cggagccatg gaagtagccc gtatcgggct ggttataacc gcctatgctt ccgtaaagaa
    19981 ttccggcctt tccatccgga ttgagataga gaccggaaat caggccatcg aacagatcgg
    20041 acgcaacttg atgcttgcca aaggccgctc ccatgtaggc ataataagcg ccgccgatcg
    20101 gggattccga attaaccagc ggatccatgg cattgaaagg attgaaactg gccaaaccgg
    20161 ttgtcataat accgggggct tggctgccct ttgcgtcact gtaaagaccg gccagttcga
    20221 tcgatgtggg ctgcccgttg gccatattat accagaggtt attgctgtcc gaattgaaac
    20281 cggcaaagac gccctggaag ttcttgttgt tcaaagacca gccggtatat tcctgggcgc
    20341 ctgcgcgcat ggcgttaact tccgcaagat aattctcata agacatggat gccgattccg
    20401 caagcgtttg gccggtgatc gaaccttccg tggcattgta ttgccaggtt gtttttgtgt
    20461 aggccaccgg attggcttcg tcaacgtagc cgtaatcagc atattgcaca gccgtgcatg
    20521 ctccttccat gcatgacgtt ttctggacat acaggcgctc attcgggaaa ttggccggat
    20581 cgccgttgat ccagtactga gtaccgctat tggtgcctga tatccacttg gtgagattgt
    20641 tggcgtacac ttttcccgcc attccgctgc tgaaagcgac attggcggtt ttctgataga
    20701 cgccgctggc ggttcctccc cagatgcctg atgtcgttcc ggaatagttg ccgaggaacg
    20761 ttccggacag attgcccttt ttggtatagg tcagaaattc tcccgagacg ggggcgtaaa
    20821 tttcgttcgc ggcccaactg gcgtccgcgc cgcccgcgtt catgtgccat atgccgtagt
    20881 cggagaaagc gccgggctgg ccggcatcgc cgaagttgcc gaaagtgccg tagcccgcaa
    20941 agtcggcctg ccagtttgtg gagccttccg gattgctgta ttctccggcg gaagatgcat
    21001 tgatgaagat gccgttggac gaatggtctt taatggaggc tgtatagcca ttgcccattg
    21061 cccaatgctt caaatatccg cccgagacaa aattgccgcc tgcaccgccg tattggatgt
    21121 tgccggacat ggcgccgagc tgtatgttcg tcatcaggtc agaagccggc atactgatag
    21181 tgccaagcat ttcgctgtaa atgtcaccgt ccgcttccca catattaagt ccggcaaagg
    21241 acattcccgc gatattggtc gatttcagga aaccggcttc gctttccgga ctgatgtaaa
    21301 aaccgtaaaa cacgccacgg gtcgagcggt tggcggaaac tctgttggtc aaaaacccga
    21361 aataagcgcc gccgtccgga gtggtgcttg ttccgttata ggaacttccg aagccggttg
    21421 ccatcgtgac cggagcttca aattccgcat gctctcccat gagggcaaga cccatgggat
    21481 tgtcttcggt tgatgtccag agcgttcttc ccgcatctcc gtctttggcg tatccgccca
    21541 tgatgccttc aaaggtgggc gccagaggaa cttcaggatc gggaatcagg cgcacggttg
    21601 tgtaccagtt atctacctgt tgcgaaaaga tttttgcgaa ttccatgttt gccggattta
    21661 cagccagctg accggtgaac ggaaacgcgt cgttgacgat gataaaatta ctgtttgcaa
    21721 cgatcggtga taccggcggc gactggtgga gatccggcca gaaaccccat gctgaacttg
    21781 acgctgatgt cgaaccctgg acttctacag ggactccatt gacaaccctc aagctttgca
    21841 atgaatcatt gggataatag atcaaatccg tgcgctggtt attgtaacgg ttgcgagcat
    21901 tttcccactg ccagccgtat ttgaccaacg agccgctggt gtcgccctcc gcccggaaat
    21961 acgctgtttc ataatccacg ccctggaaaa tccccatatt ggaattaacg cccggcatgg
    22021 catagatgtt gatattgagc gtgccttttg cggttttaaa agtctggccg gagaaaacgc
    22081 tgctgaacgc aagcgccttg gtgacttcaa aactgccgat gcccatggcc tgccagttgc
    22141 cgttattcgt gattcccacc atgtctccgg tgaagacccc catattgtcg gggttcagga
    22201 accgcccgaa gacgccgccc cagagagccc cgtcattgta agcatatccg tccacggatg
    22261 cgatggaata gcttggtttc ccggattcgt tggtttcctt ccagcccgtg tagcttgccc
    22321 atctttcggg aagcgtatcc gggtaggaac cgcccatggc cgacatccag acaccccaat
    22381 cttcgattcc atcaatgtgc gcgctcttcg tgcgcattgt gccctggaga tcgccatcca
    22441 ctttggcccc gggcgaccag tcttcggact cgacaatcga aaacgaagag gcagttcccg
    22501 gtttgacggt atgcgtcaag gttccctgat cttcgtcgtc gggaatcttc cacatgccgg
    22561 cgttggaata gagatcgccc cagacggaga atccgctgtc ttctccattc tcatcgatca
    22621 actgaccgga ggtgatcagg ccgatttcgt tgtattttgt gccgtcccag ttgcggccgt
    22681 agaagcccat catcttgcct tcgaaccatt cggggtaatc tatgcccgcc gggaaccagc
    22741 tgccgccata atagacatgg aagggattcg tctccggccc gccgtccgcg ttcgtgagca
    22801 acatattgaa cagcggtgaa ttttgattcc gcgtgtaggt tcccaatccc atcacatcag
    22861 cattcaattc gccaaacagc ggggagaacc cgcctgtcag gcctgcaatc ggataaccgt
    22921 cattaaccag tccttccgag cctggagttg tattgtaata tccccagctt ccggataaga
    22981 ctttggcgct gtaggccagc ggctttcctg accaggttcc gagtcccgtg gcctgccagg
    23041 tgtttccctg tccttcgtag acgcccagga agttgccgga cagattgccc gattttgtgc
    23101 cggttatgaa ccagccatta ttcagcgcgc cgtcaaccgg gccgtcgccc caccagtttc
    23161 cggaggcatc ggcgagccag tagccggtat cgcccatatt gccgtaattg gaatcatagt
    23221 aatatccaaa tgttccaatg ccgccggtct gcccggacca tgtggtttga ccgctctcat
    23281 ccgggttgaa acggccggca aatccctgac ggtaaatgcc ccagggctga ccctgacctt
    23341 ctttgacgat gaacagggtc tccagggact gattgtccgt ccatatgctg ccgcctgtcc
    23401 ccagcgtcgc agccagattc atgccgctat aatccattgt ctgcgcggtt gaagtataaa
    23461 ggttttctgg ggcaatcccg atgccggtca ccaccggctc ttccggatag aggccgccgt
    23521 ccatcacaag cagtccggtc tgtggataga aaggaccgga cag
//
LOCUS       DAOSIM010000007        22206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_2593_length_22206_cov_7.516862, whole genome shotgun sequence.
ACCESSION   DAOSIM010000007 DAOSIM010000000
VERSION     DAOSIM010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 22206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22206
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2593_length_22206_cov_7.516862"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..560
                     /locus_tag="PLR47_00705"
     CDS             201..560
                     /locus_tag="PLR47_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11480.1"
                     /translation="MKKHLLIFLLIFLWMAAAWLPAEPAFADPPQDLSLSYDLKTQTL
                     SASITHKSTFTGFHYIKQVQIKKNQEPLETADYDSQAGKATFVYTYKVPAKPNDLLEV
                     TAVCNLQGKKTATLKVE"
     gene            587..2392
                     /locus_tag="PLR47_00710"
     CDS             587..2392
                     /locus_tag="PLR47_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOG11481.1"
                     /translation="MTLSHPASTASPSAFRTWCCVFLLLGLLSATGPAVVVLAAGPVE
                     IVVEGVDGEARKNVLEALTLPPGLVKDGKADRLWLERFVRQAGRKVPAALEPFGYYSA
                     RAVVLIETPEADEYRLRVQIEPGQPAVVSGVSVTVAGSGASETTLAALAADFPVRKGD
                     ALSHPLYEKAKQALKSRAEELGYLDAAYTVHEIRVDEAGLSAVITLVLETGEKYFFST
                     TTIEGAPDYPDAFLRRYLAFQPGEAFSYTRMAESQLQLNNSERFRRVSVQADKEQSRE
                     GKVPVRVLLTPVPRITLRPGIGYGTDTGARFALRCRDLNMLHRGHELDSHLYISQRLQ
                     GLATSYIVPDGRDIRTFTSAQINLQQEEITTYDSRIIAMELARTHSFTPRRQGSAYIK
                     FQHEEFTIGSQEATSRYVLPGVRFSDNHYDNLIRPDRGFRYALDLRGTHQALGSTTRL
                     LQFIGEASHILPLPWRLSFLTSAKAGFTVFTDEFSDLPPSLRFFAGGDQSVRGYAYQS
                     LGPRDAEGNVKGGKHLLTGCVELQRDILEDWAVSVFYNAGNAFDTFSDLTLYQAGGFG
                     IHYHTPVGALNLYVARQIRVDDPEFRFHFTVGFEF"
     gene            2398..6366
                     /locus_tag="PLR47_00715"
     CDS             2398..6366
                     /locus_tag="PLR47_00715"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOG11482.1"
                     /translation="MLKYLILVVVLGVPAVLLAAAGWLFGTEAGGKKLLETVSSFTEV
                     NIRAETVEGNLADSLRLTGTVIEWPQGSLRIGKLRLSMRPQDLMAGHLRFSLLSADQV
                     SVFDNSPDKQPELVWPRAKGWITFVSGSIGRLAVRNLTYRRPNEEPVQITALAAAAVW
                     KNSSLSVSDLRLTSSFGVLEGSVLAGFHRPLLKAKLAATPAHPAAGVDSIRLDAAFGP
                     GKKPGEMKGSLQIHGLQNTRPVLRISSDIGMTPAGLTFAKILLRRGNRPGSIAGEGFL
                     AFKGPTPHAALKVQAENMDLSDELKVPAKFNGSLLLDGTGRQYQGRVVLSNTADTRHA
                     FRLAGDYSGNAEGATLSPIQATALGGSLNGRLKIGWTDGLKISGALSGKNLNPAKWNT
                     DWAGIVHFDLEGDLSAPAEAPPSGSFNLTLKESRLHGRQLTGDLRATFADNDIHIQRL
                     ALRGKGFQFAARGSVADQLDFSAEISDLARLIPHTSGSLTASGWARRRHDRFSGVVAA
                     RGRSLAADGWSIESGNFSASLEDREKSPFALTADFSRLRFRSLTTDTLRVRGGGTALS
                     HTLAAVMRRGPHETRLDLSGARREKIWKGKIIRLEGTDGVGPWRLSSPSALEISAERL
                     ALGPMEITGRGAEAFRASAVLSGNPLTGPVDLTWNDVDLARANAWTDHGIATGACGGI
                     IHATLLPSNRIDLALEATLSGTLSAQGQTVEVRQGKITLNANKRGTRLDVKFEPSDGG
                     WLQGSFFSSQPAKQSLPDTGQWDVRWRELDLKRFSASLPRRTKLEGRTSGEMEGKLLS
                     GGRFSCAGRAGLDHSKIHRRGQRGDVSIDFRRASAEWVWRKDTLACDVAVAMADHGEL
                     KGKIRLPLAARFGWTLNERGPLQGSLAGQVQEKGALGVLFPGLVQESRGTLDLDISVS
                     GTPADPLFEGRIRLSDAGGYLPSAGITLQDVRIEARPSKDGLVIDSFRAASGPGYVDG
                     SARIHLKGMQIAGFEGRIDGERFQTVHVPELHVQSSPRLTFWGTPEKISVRGEVLLPL
                     LEVNGSQSQGPAASSSDVIREGKTKSPGRKPSVGLDVQVKMALGDEAHFNASGIDAKL
                     GGEMDISFSDPERISGRGEIRVVKGRFRTYGVNLEIVRGHLFYAGGPVNQPALDILAL
                     RKIGDVKAGVAVSGSLPKPVIKLYSEPQMQDMDILAYIVLGHPLGADTKQAGLLATAA
                     SAVLTSQQAEDLLEQIQGYLGLASLEISSDVLEKSNRMGYRRLKVTPEGTGGSAQSAS
                     ETILVVGKYLTPQLYISYGRSLFSGGNLFFLRYDISKNWQVESQMGQESGVDLYYKLE
                     FH"
     gene            6382..7740
                     /locus_tag="PLR47_00720"
     CDS             6382..7740
                     /locus_tag="PLR47_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOG11483.1"
                     /translation="MPSPHFDLVITGGTLVTMSAKMEIVENAVIGIADGRIARVGSHL
                     SLSADRVIDAGGGLILPGLINTHTHLPMVCFRGLADDLPLMAWLNHHIFPMEARFVNR
                     QMAYDGALLAIAEMILSGTTTCCDGYFFENQIAQAALDCGMRAVAAQGFADFATPDHP
                     QYEKMMAAAERFVERWLPHAPMITPAFFCHSPYTCSPRTLVNVKAAAREAGILYLTHL
                     LENRDEIGMIEKRYGRKPVQHLHHLGVLDDRTVAVHCNWTTPEDIELFADLGVRVSHN
                     PSSSMKLAAGIAPVPLMLEKGIAVGLGTDGAASNNDLDMFQEMDLSAKLHKAALLDPT
                     VMSAETVLKMATIDGARVLGLDRLIGSIEAGKQADIIILDLNQPHLTPLYNPYSQIVY
                     AARGADVKASIIGGKPVMENRRLLTIDLQDAMRNVGEIAAHIQAENQSTIRTSPVDVM
                     TP"
     gene            complement(7778..8782)
                     /gene="msrB"
                     /locus_tag="PLR47_00725"
     CDS             complement(7778..8782)
                     /gene="msrB"
                     /locus_tag="PLR47_00725"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOG11484.1"
                     /translation="MTQTEKEAVATLAGGCFWCVASDMKKLPGVTRVVSGYAGGSGKN
                     PTYGNYMAQGHLEAVQVHYDPGVVTYGQILHYFLKHIDPTDAGGQFADRGSGYRSAVF
                     YNNEAERKTAAGALQSLAASGKFGKPIVTELKLFTGFYPAEEYHQDYDAKNPLRYRAY
                     RSGSGREGFLREVWKKDRDNGPATAPPYRKPDDKILKKTLSPMQYDVTQKNATEPPFQ
                     NEYVRNKKEGIYVDVVSGEPLFSSRDKFDSGTGWPSFTRPLELSNITTKEDRSFFATR
                     TEVRSRHADSHLGHVFPDGPGPAGLRYCMNSAALRFIPREDLEKEGYGRYQKLFSQDK
                     "
     gene            complement(9058..9342)
                     /locus_tag="PLR47_00730"
     CDS             complement(9058..9342)
                     /locus_tag="PLR47_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11485.1"
                     /translation="MKKMMVFVCAFLLFAAAAYAAGPRTYQVTGPVLDVKGDVIVVKK
                     GSDNWEVAKNAATRVAGDLKVGSRVTIEYTMTATKIDVKEDKKADAKKKK"
     gene            complement(9380..9703)
                     /locus_tag="PLR47_00735"
     CDS             complement(9380..9703)
                     /locus_tag="PLR47_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOG11486.1"
                     /translation="MLKKKRFLFLKNGFTLAALMLCAALIAQTAAFAQAAEKSAGEKL
                     PPAVRVNINKADQATLEKLLGIGPVKAKAIIDGRPYRNIEDVMNIKGIKGKTYDAIKE
                     HIVVK"
     gene            9863..10126
                     /locus_tag="PLR47_00740"
     CDS             9863..10126
                     /locus_tag="PLR47_00740"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HOG11487.1"
                     /translation="MTDPKKQKLRLDYPCLWTYKIIGRNPDEMQSAVHEIIRDRPCRV
                     SLSRQSETARYVCLNVELTVESESHRLVLYEELKAHRAIQVIL"
     gene            complement(10261..11553)
                     /locus_tag="PLR47_00745"
     CDS             complement(10261..11553)
                     /locus_tag="PLR47_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HOG11488.1"
                     /translation="MQKEYDVIVAGTGTGGSTVAREMTRRGKKVLLLEAGGRWEWMGS
                     TMTIAMILKHCGLVRSREGRTVTFADNYGGLSNLAGGCAAPPPKSVFDPVGIDLAEEA
                     GEAKKDLWIQKQPDELVGRENLRLVEVANSLGYHWGKMDKFVDPKRCRASGDCMLGCR
                     TGAKWTARVYGDEAVSQGADLHLHTRITEVIVENNRVVGVKGTRRGVPVKYFGKFVVL
                     STGIDNAHILRRAGISRAGKGFCCDWLQFVGAEIPGMNTVGATPMSVGSLEFYESDGI
                     AIIPVFPNWSMYAATLAMAGPKSLLNFPRFWKLSGIMVKVRDETAGELFQGVSFSKKV
                     SEADRMKLDKGVDIIKKVFRKAGAKDSTILPLNPQGAHPSATCRIGDVVDRNLETEID
                     HLYCCDASVLPSSLGLPVMWTVVALGKRLGGHLAARIG"
     gene            complement(11537..12481)
                     /locus_tag="PLR47_00750"
     CDS             complement(11537..12481)
                     /locus_tag="PLR47_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG11489.1"
                     /translation="MRASTKALMAKHGWRIDRAIHNYLYFSFYYPYVRVVYHFFAFLA
                     RHLSWFKPLNLLIRAAFARYHAKVISGEDAVKILELDEDVTAVSEKNKQIVPFDHAYK
                     ILLQEPDFIAVMDCPCKKSLNAPEWTINSCISVGKKTSQFWLDRCGAKYHARKISQKE
                     AVDLVKKFREKGYVTQAFFKVATGGSTGIICNCHIDTCVSLQATRFARRFDPGLTMNA
                     DSGYSMHRDEALCRQCGACARICQFEAIVQSGDQQTYHKEICLGCGLCMEHCPEQAIR
                     IYRDPQKTVPLDMDIVKNEYASVQKIRDSSARSDAKRI"
     gene            complement(12592..13149)
                     /locus_tag="PLR47_00755"
     CDS             complement(12592..13149)
                     /locus_tag="PLR47_00755"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOG11490.1"
                     /translation="MINVIWICCAAYLAGSVNFAILFFKIAGRADPRLSFSGNAGTTN
                     VYRLAGMPWAVLVFALDIGRAMAVAALAAYFLDGGWLIWAGFFLVLGNSFPCFHGFRG
                     GKGVANYLGFTVLTVPVAALSAAWLWVLAYWLKRAPFIASFFMTAALSAGHVVSSSGD
                     WLAAAGAAAIFALIVFNHRKNIREY"
     gene            complement(13146..13985)
                     /locus_tag="PLR47_00760"
     CDS             complement(13146..13985)
                     /locus_tag="PLR47_00760"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase dehydratase
                     domain-containing protein"
                     /protein_id="HOG11491.1"
                     /translation="MKQIFSFKLPIPPYLRDHCVEGQAVFPAVESLALLAREVQNRRP
                     GALVNRLADADFPKMLALPEEADELPVRIEIEERENGIHASLQTTVSAKTASIRRALV
                     HARVTFVRKDAPPEAPMPFQASGTTGDDSIPVAADSVYRKWIPFGPSYRNLVGTLAVS
                     REGVSAEISGGSGEADDTPLGSPFAPDAAMQAACVWGQRFAGIAAFPTGFDHRIIYRP
                     VKNGETCFARVIPVNGNRGFLVFDVWIFDRREMLCESIRGLRMSDIFRGRLKTPRRIG
                     EGE"
     gene            complement(13982..14467)
                     /locus_tag="PLR47_00765"
     CDS             complement(13982..14467)
                     /locus_tag="PLR47_00765"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOG11492.1"
                     /translation="MKPKPFLPETCKMDERFVRDRTTGLVWHRTPYRVLYADTDRSQV
                     VYHSNYLRYFEVGRTSLMRDLAYPYREIEESGYVYPIYDLGISFYQPLVYDDVMHIHT
                     RPVNLERVRLQFDYVVTRADAGRLVCTGFTRHCAANAAGRPVAVDPKTIRLWQTFPDP
                     V"
     gene            complement(14469..15734)
                     /locus_tag="PLR47_00770"
     CDS             complement(14469..15734)
                     /locus_tag="PLR47_00770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOG11493.1"
                     /translation="MKPPQNRRVFIVGYGAATPLGSTFAKTWERAVQGDAGFRKVTRC
                     KVESACDIVGEIPDWSPRNLDFSDAREVYNWNAAFVLLTMAVCKEALQNAGIVMDETI
                     APRMACLIGSALNGTDAFRAAMHDLEHRGPLRVSPYLLPNLCANLPSGKAGMLLKFTG
                     PIFSPQGACASGNHAIGIGARMIRDGDCDFVLAGGADAPILPELIHGFTNMNATIKVH
                     AGDRAAADPAQASRPFSIDRRGFVLSEGAGVVVLAAEEVIRAHGLEPRAEVLGVGWTS
                     DAYHYTSPNPATIVRALRETIADAGLQPADVQYVNAHGTSTAKGDRTEIQCLREVFGG
                     LMEMIPVSSNKSQLGHTLGATAAIEAALAVEGMRRGILLPTINHLPDPEFADIDVVPN
                     TARKQTHEIVLSNAFGFGGTNCCVIFRGV"
     gene            complement(15753..17000)
                     /locus_tag="PLR47_00775"
     CDS             complement(15753..17000)
                     /locus_tag="PLR47_00775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOG11494.1"
                     /translation="MSGKTLILGYDAVSSLGTDLEIQWRRAAAGESGIGPLTRFPYGD
                     DFPVRVAGEVEDVDVRPYPFLQPREMAHWTSPIFKYALLVVHRALAKSGIDITPDVAP
                     RVAVTFSSAVGGQDAVLKADRQMIAENKLPHPFANPNSCINMVGGKVSILTGATGPIC
                     ATITACATGVTSMIIGGMMIQQNLADVVIAGAVDFPLVEPILAGFATMNGAYRPKEGR
                     PHEAPEKVSRPFSANRRGFVVSEGAGAVILASPDFARAHGLAARIEIAGFGMTSDAGH
                     FVAPNPATVERCIAVAIADAGLTSAEVDAVNAHATSTRIGDKVEADALTQIFGAKAPP
                     TSANKSQLGHAMGASSAIETILAIEGMLRDTVLPTVNYEPDPAIHLDCVPGTARKLCQ
                     EFVLKNAFGFGGCNSCLLLRRIA"
     gene            17292..20009
                     /locus_tag="PLR47_00780"
     CDS             17292..20009
                     /locus_tag="PLR47_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HOG11495.1"
                     /translation="MESIICKHWHHCPGSEVTTLLNTDPEKGLSPFEAGHRLKKYGPN
                     AVTVKKQQGPLMRFLLQFHQPLIYILMAAGAVTLFLQEWVDSSVILGVVIINAVIGFV
                     QESKAQSAIESLKKMMTTQTTVLRDGRWQEMDAVNLVIGDIVQIQSGNKVPADVRILR
                     CRDLQVDESALTGESVPVIKKEETLDPETILADRRNMAYAGTLVTYGQARAVVVATGD
                     KTETGRISELISSAADLSTPLTIKIASFSKILLIAILVLAAATFAVGMWRGESAVDMF
                     MAAVALAVGAIPEGLPAAVTITLAIGVNRMAKKRAIIRKLPAVETLGSTTVICSDKTG
                     TLTENQMTVKKIFTGGASYDVEGNGYAPEGHLSANGKELTGDLPPGLRQTLTAGLLCN
                     DSRLIQEEDAWRIEGDPTEGALITAAGKAGLTPEKTGADMPRLDEIPFESERQFMATL
                     HRSTAGGKNILYIKGAVEKILEACADAMASEGGKTAFDKENLPARVDAMASEGLRVLA
                     LACKETDKDHLEVEDCLSGLTFLGLQGMIDPPRKEAIEAVRSCRRAGIAVKMITGDHA
                     VTATEIARQLNIGSLKGKDEAAPRTLTGKELEKLTDEELIAAAEDTSVFARVAPEQKL
                     RLVEALQARSHIVAMTGDGVNDAPALKQANIGIAMGITGTDVSKEAADMVLTDDNFAS
                     ITRAVEEGRRIFDNLVKFITWTLPTNGGEGLVILAAIVTGVTLPILPVQILWINMTTA
                     VLLGLMLAFEPGESDVMNYPPRDPQSPILNATVITRILLVTAIMLAAVFGIFVWQKQQ
                     GYSLAEARTVAVNLFVMIELAYLFNCRSLTKSMFRIGFFSNPWVLAGSAAMIVLQLLY
                     TYTPAMNRVFQSEPISGRDWLMIIAISIIVYVVVGAEKWLRRGAFAKTH"
     gene            20074..20880
                     /locus_tag="PLR47_00785"
     CDS             20074..20880
                     /locus_tag="PLR47_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HOG11496.1"
                     /translation="MDAQKIPGKVIIGAVIGFAAILLLLSTYFSIEAGDRGIVLRFGQ
                     VNRVVEPGARFKIPFAEEVVRMSVRVQKTTTKTEAASRDLQTVQTTMVLNYSLDPART
                     GEMYANIGLNFNERVIDPAVKESFKAAAARYTAEELISKRETLKTEVRNYLRERLHVF
                     GINIVELSVTDFEFSAEFNRAIESKQTAEQNALRAKRDLERIKVEAEQKVTSAKAEAE
                     ALRLQRQVISPELIQLRQIEAQIKAIEKWDGKLPNVTGGAVPFIQVEKPK"
     gene            complement(20912..21907)
                     /locus_tag="PLR47_00790"
     CDS             complement(20912..21907)
                     /locus_tag="PLR47_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HOG11497.1"
                     /translation="MKTVSTLLDAEWRERYRRISSLNIRSSIYDLTNRCNLRCKGCFF
                     FSSGEHHVQEEMDIRRWEAFIDREKIRGVNLAILIGGEPTLCMDRLEAFYRRVPTYCA
                     TNGLIKVPRDRFPDMMVGISLWGGGEDEKILRGRDTFAVSSKNYEGDPCAYYLYTLTP
                     RQVGKTEPVIRRIRDAGLKVHLQLLSNDEGVDGFSWRPEELSDLRFELDEMLDHYPRT
                     VISCRYYHEIITTGLMQGRRFGWRECPSVTESLDHRQPPVRRLIHFYRWAADLQTVHR
                     CCTSATRDCSTCKDGAAHMSWVMVNKRDHMNATRDLHNWIEVYEMFAKLYKFIPW"
     gene            complement(21954..>22206)
                     /locus_tag="PLR47_00795"
     CDS             complement(21954..>22206)
                     /locus_tag="PLR47_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11498.1"
                     /translation="RTDLNWDRSAACCVPDTDCADCRHYAAGSAVVTARLYRHVGDED
                     AFKSWLDYVDTYLAVWVMGYEKGENLCNKIIHPPRAAAG"
BASE COUNT         4626 a         6730 c         6421 g         4429 t
ORIGIN      
        1 cggaaagaga aaatggaatc cgatggtaaa ggcgaattgc aggcgggcaa ggagcaacgc
       61 gtccatagct tcctccggga ttgtagtgga aggaagaata tcgaattgtc cctccttttt
      121 caatccctat tttcctgttg tcatttccgg accaattgta ttactgtagg accaacaaaa
      181 tcctttcagg agaatgcgac atgaaaaaac atcttttgat ctttttgctg attttcctct
      241 ggatggccgc cgcctggctc ccggctgagc ccgccttcgc cgatcctccc caggaccttt
      301 cgctgagtta cgacctgaag acccagacgc tttcggcatc catcacgcac aaatcaacgt
      361 tcaccggctt ccactacatc aagcaggttc agatcaaaaa aaatcaggag ccgcttgaaa
      421 ccgccgatta tgattcacag gccggcaaag ccaccttcgt ctatacctac aaagtgccgg
      481 cgaagccaaa cgatcttctg gaagtgacgg ccgtctgcaa ccttcagggc aaaaaaacgg
      541 cgacgctgaa agtggaatag aaaaccatca ggaatccatc gagatcatga ccctttccca
      601 tccggcgagt acggcgtccc cttcggcctt ccgaacctgg tgctgcgttt ttctcctttt
      661 gggccttttg tccgcgacgg ggccggccgt cgtcgtcctg gccgccggcc cggtggaaat
      721 cgttgtggaa ggcgttgacg gagaggcccg gaaaaatgtt ctggaagcgc tcaccctgcc
      781 tcccggcctg gtaaaggatg gaaaagcgga ccggctctgg ctggagcggt ttgtccgcca
      841 ggccggacgg aaagtgccgg cggcccttga acccttcgga tattattccg ccagggccgt
      901 cgtcctgatc gaaacccccg aagcggacga ataccggctg cgggtgcaaa tcgaaccggg
      961 tcagccggcc gtcgtttccg gggtcagcgt caccgtcgcc ggaagcggcg cgtcggagac
     1021 aacgctggcg gcgcttgccg ccgacttccc tgtgcgaaaa ggcgacgcgc tgtcacatcc
     1081 tctttacgaa aaagccaagc aggcgctgaa atcccgggcg gaagagctgg ggtatctgga
     1141 cgccgcctac accgttcacg aaatccgggt agatgaggcc ggcctgtcgg ccgtcatcac
     1201 gctggtgctg gaaaccggcg agaaatattt tttctccacg acaaccatcg agggcgcccc
     1261 cgattatccc gacgcctttc tgcggcgcta cctcgccttt cagcccggag aggctttctc
     1321 gtacacccga atggccgagt cccagcttca gttgaacaac tcggagcggt ttcgccgagt
     1381 gtcggtccag gccgacaagg agcagtcccg ggaaggaaaa gtgccggtgc gggtgctcct
     1441 gacgcccgtg ccgcgcatca cgctgaggcc gggcatcgga tacggaacgg atacgggagc
     1501 gagattcgct ttgcgctgcc gggacctcaa catgcttcac cgggggcacg aactggattc
     1561 gcacctgtac atctcccagc ggctgcaggg actcgcgacg agctacatcg ttccggacgg
     1621 cagggacatc aggactttca cctccgcgca aatcaacctt cagcaggagg aaatcaccac
     1681 ctacgacagc cggatcatcg cgatggaact ggcccggacg cacagcttca ctcccagaag
     1741 gcagggttcg gcgtacatca aatttcagca cgaagaattc accatcggca gccaggaggc
     1801 aacctcgaga tacgtccttc cgggcgtccg gttctccgac aaccactacg acaacctgat
     1861 tcggcccgac cgcgggttcc ggtatgcgct cgatctgcgc ggcacgcacc aggccctggg
     1921 ctccacaacc cgcctgcttc agttcatcgg cgaggcgagc cacattcttc ccctgccgtg
     1981 gcgcctgtct ttcctgacca gcgccaaagc gggcttcacg gtttttaccg acgagttttc
     2041 cgacctcccc ccgtctttgc ggttttttgc gggcggcgat caaagcgttc gcggctacgc
     2101 ctaccagtcg ctgggtcccc gcgacgccga gggaaatgtc aagggcggca agcatctgct
     2161 gaccggctgc gtggaactgc agcgcgacat tctggaggac tgggcagtct ccgtgtttta
     2221 caacgcggga aacgcttttg acactttttc cgatctgacg ctgtatcagg ccggaggctt
     2281 cggcattcac taccacacgc cggtcggcgc cctgaattta tacgtcgccc ggcagatccg
     2341 cgtggatgac ccggaattcc gctttcactt taccgtgggg tttgaatttt gaagcgcgtg
     2401 ctgaaatatc tcatcctcgt cgtcgttctg ggcgtgccgg cggtccttct cgcggccgcg
     2461 ggctggcttt ttggcaccga ggcgggtgga aaaaaactgc tggaaaccgt ttcgagcttc
     2521 acggaagtga acatcagggc ggaaaccgtc gagggaaatc tggcggattc cctccgcctg
     2581 accggcacgg tcatcgagtg gccgcaggga agccttcgaa taggaaagct gcggctttcc
     2641 atgcggccgc aagacctgat ggcgggccat ctgaggtttt ctctgctttc cgccgaccag
     2701 gtatccgttt tcgacaactc tccggacaaa cagccggagc tggtctggcc gcgggccaaa
     2761 ggctggatca cgttcgtgag cggcagcatc gggcgcctgg ccgtcaggaa tctgacctat
     2821 cgccgcccga atgaagaacc ggttcaaatc acggctcttg ccgcggccgc cgtctggaag
     2881 aattccagtc tgtccgtaag cgatcttcgc ctcacctcgt ctttcggcgt tttggaggga
     2941 agcgttctgg ccggtttcca tcgtcccctg ctgaaagcca aacttgccgc aactccggcg
     3001 cacccggccg ccggcgttga ttccattcgg ctggacgcag ccttcggacc gggaaagaag
     3061 ccgggagaaa tgaagggaag ccttcagatt cacggccttc aaaatacccg gccggttttg
     3121 cggatatcat cggacatcgg catgacgccc gccgggctga cctttgccaa aatccttctg
     3181 cggcgcggga atcgtccggg ctccattgcc ggcgagggct ttctggcctt caaaggcccg
     3241 acgccgcatg cagccctgaa ggttcaggcg gaaaacatgg atctttccga cgagctgaaa
     3301 gttcctgcta aattcaacgg ctctctcctc cttgacggaa ccggccggca atatcagggg
     3361 cgcgtcgttc tgtccaatac ggcggacacc cggcatgctt ttcgtctggc gggcgattat
     3421 tcagggaacg ctgaaggcgc aaccctgagc ccgattcagg caacggcgct ggggggctcc
     3481 ctgaacggac ggctgaagat cggatggacg gacggcctga aaataagcgg cgcgctttcg
     3541 ggaaaaaatc tcaatccggc aaaatggaat accgactggg cgggcattgt ccatttcgat
     3601 ctcgagggcg acctgtccgc tccggcggaa gcgccgccca gcggctcttt caatctcacg
     3661 ctgaaagaaa gccgcctgca cggccggcag ttgacgggag atttgagggc aacctttgcg
     3721 gataacgata ttcacattca gcgcctggcg ctcaggggaa agggctttca gttcgctgcc
     3781 aggggaagcg tcgcggacca attggatttc agcgctgaaa tcagcgatct ggcgaggctg
     3841 attccccaca catcaggatc gcttacagct tcagggtggg cgcgccggcg acatgaccgg
     3901 ttcagcggcg tcgttgccgc ccggggccgc tctctcgcgg cggacggctg gtccattgag
     3961 tccgggaatt tcagcgcctc cctggaagac cgggaaaagt ccccctttgc gctgaccgct
     4021 gatttcagcc ggcttcgctt ccggagcctg acgacggata cgctgagagt gcggggcgga
     4081 ggaaccgccc tttcccatac gctggccgcc gtgatgcgcc gcggccccca cgaaacgcgc
     4141 ctggacctgt cgggcgcccg gcgggaaaaa atctggaagg gaaaaataat tcgtctggaa
     4201 ggcacggacg gagtcggccc ctggaggctg tcctctccct ccgccctgga gatatccgcc
     4261 gaacgactgg cgctggggcc gatggaaatc accggccggg gggcggaagc gtttcgggcg
     4321 tccgcggttc tttccggaaa ccccctgacc ggtcctgtcg atctgacctg gaacgacgtc
     4381 gatttggcca gggccaacgc ctggaccgat cacggcattg ccaccggcgc gtgcggcgga
     4441 atcattcacg caacgctgct gccctcaaac cgcatcgacc tagccttgga ggcaaccctg
     4501 tccggaaccc tgagcgcgca aggacagacc gttgaagtcc ggcaggggaa aataaccctg
     4561 aacgcaaaca agcggggaac ccggctggac gtgaagttcg agccgtctga cggcggatgg
     4621 ctgcagggct ccttcttctc atcccagccc gcaaaacagt cgcttcccga cacgggccaa
     4681 tgggatgtgc gctggcggga gctggacctg aaaaggtttt cggcatcgct cccccgccga
     4741 acgaaactgg aaggcagaac gtcgggggag atggaaggca aacttctgtc cggcgggcgc
     4801 ttttcctgcg cgggccgggc cgggctggac cactcaaaaa tccaccggcg ggggcagagg
     4861 ggcgacgtca gcatcgattt tcgccgggca tccgcagaat gggtctggcg gaaagacact
     4921 ctggcgtgcg acgtcgccgt ggcgatggcg gaccacggag agctgaaggg aaaaatccgc
     4981 ctgcccctcg ccgcgcgctt tgggtggacc ctgaatgagc ggggaccgct tcagggttcg
     5041 cttgccggac aggttcagga aaaaggagcg ctgggcgttc ttttccccgg gctggttcag
     5101 gagagccgcg gaacgctcga tctggatatc agcgtgagcg gcaccccggc ggacccgctg
     5161 tttgaaggcc gcatccgcct gtccgacgcc gggggctatc tgccttcggc cggcatcacg
     5221 cttcaggacg tccggatcga agcccgtccg tcgaaagacg gcctggtcat tgattccttc
     5281 cgggcggcct ccggccccgg ctatgtggac ggttccgcgc ggattcatct gaaagggatg
     5341 cagatcgcgg gttttgaagg acgcatcgac ggcgagcggt ttcagaccgt tcacgttccc
     5401 gaacttcatg tgcagtcttc tccccggctg accttttggg gaacgcctga aaaaatatcc
     5461 gtccggggag aagtcctttt gcccctgctt gaggtcaacg gatcgcaatc gcagggcccc
     5521 gcggcatcca gttccgatgt gatccgggaa ggaaaaacaa aatcccccgg ccgcaagccg
     5581 tcggtcggcc tcgatgttca ggtcaaaatg gcgctgggcg acgaggcgca tttcaacgca
     5641 tccggaatcg acgcgaagct gggcggcgaa atggacattt ccttcagcga tccggagcgc
     5701 atttcgggcc ggggcgaaat ccgcgtggtc aaaggccggt tccgaaccta cggcgtcaat
     5761 ctggaaatcg tccgcggcca tcttttctat gcgggaggtc cggtcaatca gccggcgctg
     5821 gacatcctgg cgcttagaaa aatcggagac gtgaaggcgg gcgtcgcggt cagcggttcg
     5881 cttcccaagc ccgtcattaa actgtactcc gaaccgcaaa tgcaggacat ggacatactg
     5941 gcctacatcg tgctggggca tccgctcggc gccgacacaa agcaggccgg tcttctggcc
     6001 acggcggcct ccgcggtttt gacctcgcaa caggccgaag atctgctgga acagattcag
     6061 ggctatcttg ggctggcctc gctggaaatc tcttcggatg tactggagaa aagcaaccgg
     6121 atgggataca ggcgtctgaa agtgacgccc gaggggacgg gcggttcggc gcaaagcgct
     6181 tcggaaacca tcctggtcgt aggcaaatat ctgacgcccc agttgtacat cagctacggc
     6241 cgctcgctgt tttcgggggg caatttattt ttcctgcgct acgacatttc caaaaactgg
     6301 caggtggaaa gccagatggg gcaggaaagc ggcgttgacc tctactacaa actggaattt
     6361 cactgacgga gagagaaaat catgccatcg cctcattttg acctggtgat tacgggcggc
     6421 actctggtga ccatgtccgc caaaatggag attgttgaaa acgccgtcat cggcatcgcg
     6481 gacggccgca tcgcccgggt gggctcacac ctttcccttt cggccgacag ggtcatcgac
     6541 gccggcggcg gcctgattct gccggggctc atcaacacgc acacgcatct gccgatggtc
     6601 tgcttccggg gcctggcgga cgaccttccg ctcatggcat ggctcaacca tcacatcttt
     6661 cccatggaag cgcgcttcgt gaaccggcag atggcgtacg acggcgccct gctggcgatc
     6721 gcggaaatga tcctgtccgg aaccaccacc tgctgcgacg ggtatttttt tgaaaatcaa
     6781 atcgcccaag ccgcgctgga ttgcggcatg cgcgcggtgg cggctcaggg attcgccgat
     6841 tttgccacgc cggaccaccc ccaatacgaa aaaatgatgg cggccgccga acggtttgtc
     6901 gagcgctggc ttccccacgc gccgatgatc acgcctgcgt tcttctgcca ttccccctac
     6961 acctgttcgc cccggacgct ggtcaacgta aaggcggccg cgcgcgaagc cggaatcctt
     7021 tacctgaccc accttctgga aaaccgggac gagatcggca tgattgaaaa acgctacggc
     7081 cggaagccgg tgcagcatct gcaccatctg ggcgtgctgg acgaccggac cgtcgcggtg
     7141 cactgcaact ggaccacacc cgaggacatc gagctttttg cggatctggg cgtccgcgta
     7201 tcccacaatc cgtccagctc catgaaactg gccgccggca tcgcgccggt gccgctgatg
     7261 ctggaaaaag gcatcgcggt cggcctgggc acggacgggg ccgccagcaa caatgatctg
     7321 gacatgtttc aggaaatgga tctttcggca aaactccaca aagccgctct gctcgatcca
     7381 acggtcatga gcgccgaaac cgtcctgaag atggcgacga tcgacggcgc ccgtgttctc
     7441 ggactggatc ggctgatcgg ctccatagag gcgggaaaac aggcggacat catcattctg
     7501 gatctgaacc agccgcacct gacgccgctt tacaacccct attcccagat tgtgtatgcc
     7561 gcaagaggag cggatgtaaa agccagcatc atcggcggaa agccggtcat ggagaacagg
     7621 cgcctgctga cgatcgatct gcaggacgcc atgcgtaatg tcggcgaaat cgccgcgcac
     7681 attcaagcgg agaatcaatc cacgatcagg acgtcaccgg ttgatgtcat gacgccatga
     7741 atattgccgg atggagagca acggtttgaa accgttgcta tttgtcctgt gaaaataatt
     7801 tctgataacg gccgtagccc tctttttcca gatcttctct gggaatgaaa cgcagggcgg
     7861 ctgaattcat gcagtagcga agacctgcag gccccgggcc gtccggaaac acatggccca
     7921 gatgggaatc cgcgtgccgg ctgcgaactt ccgtgcgcgt cgcaaagaag ctgcggtcct
     7981 ctttcgtcgt gatattggac aactccagcg gccgagtgaa actgggccag ccggtgccgg
     8041 aatcaaactt gtctcgcgaa ctgaagagcg gctccccgga aacgacatca acataaatgc
     8101 cctctttttt gttgcggaca tattcgtttt gaaagggcgg ctccgtcgcg tttttctgcg
     8161 taacgtcgta ctgcatcggg gaaagagttt ttttcaggat tttgtcgtcc ggttttcggt
     8221 agggcggcgc cgtagccggc ccattatccc gatccttctt ccaaacttcc cgaagaaatc
     8281 cttcccggcc cgacccgctg cggtaagccc tgtagcgcag cggattcttg gcgtcatagt
     8341 cctgatgata ctcctccgcc ggatagaatc ccgtaaaaag cttcagctcc gttacaatcg
     8401 gcttgccgaa cttgcccgat gcggccagag attgcagagc cccggcggcc gtctttcttt
     8461 ccgcctcgtt gttgtaaaag acggcgctgc ggtatccgga accgcgatcg gcaaactgac
     8521 ctcccgcgtc cgtgggatca atatgcttca gaaagtaatg gaggatctgc ccataggtca
     8581 ctacgcccgg atcgtaatga acctgcacgg cttccaggtg cccctgggcc atatagttcc
     8641 cgtaagtcgg attttttccc gaaccgcccg catagcccga cacgacacgg gttacgcccg
     8701 gcagtttctt catatcggaa gcgacacacc agaagcaccc gccggccagc gtcgcgactg
     8761 cctctttttc tgtttgggtc ataccgttca ttttttccgc ccccttcacg ggctgtgtgt
     8821 tgccgccctg acaggccagc gcaaaccaca gcaatccagc ggcaatccat gcctttttca
     8881 ttttgtccgt cccgtctttt gaatcctgtt ttccttaaat aagagtttaa ggacgattgc
     8941 gggaatgtca aaccggcaaa aaaaagaggc cattcatgac ctcttttttt gcgccaccgc
     9001 ggcaaccgct ttcattaatc gggctaccga agcggctttg tgtgtctgtt gaaatcgcta
     9061 tttcttcttt ttggcgtccg ccttcttgtc ttccttcacg tcaatcttcg tcgcggtcat
     9121 cgtgtattca atcgtgaccc gggagccgac ctttaggtcg cctgcgaccc tggtggcggc
     9181 atttttcgcc acttcccagt tatcgcttcc tttcttgacg acaatcacat cccctttcac
     9241 atccagcacc ggcccggtca cctgataggt tctggggccc gcggcatagg ccgccgccgc
     9301 aaacagcaaa aacgcacaaa cgaaaaccat cattttcttc atcaatccat cctcctttgc
     9361 agtttttgaa acgaaacgct tatttgacga cgatgtgttc cttgatcgca tcatacgtct
     9421 ttcccttgat gcccttgatg ttcatgacat cttcaatgtt ccggtagggc cggccgtcga
     9481 taatcgcttt ggccttgacg gggccgatcc cgagcagttt ctccagcgtc gcctggtcgg
     9541 ccttgttgat gttgacccgc accgccggag gcagtttttc cccggctgat ttttcggcgg
     9601 cctgcgcgaa tgcggccgtt tgcgcaatca gggcggcgca cagcatcaag gcggcaagcg
     9661 taaaaccatt tttcaaaaac aaaaaacgtt ttttcttcag catgagacct cctcgtgtga
     9721 tgttttttgt tgaaggatcc ggtgcaaaaa cttctagcag agaccggcgg acttgtcaaa
     9781 gggaatgtcg ggcaacaaga gggatgaacc tcttgacttt atttgctttt ccggatagtt
     9841 tctcccggaa agcaggcaga ccatgacaga tccaaaaaaa cagaagcttc gattggacta
     9901 tccgtgcctc tggacctata aaatcatcgg ccggaatccg gacgagatgc aaagcgcggt
     9961 tcacgaaatt atccgggacc gtccctgccg ggtgtctctg tcgcgtcaaa gtgaaaccgc
    10021 ccggtacgtc tgcctcaacg tggagctgac cgtggagagc gaatctcacc ggctcgttct
    10081 gtacgaggaa ctcaaagccc accgggccat tcaagtgatt ctctgaaaaa accgttttgc
    10141 cccggcacgc tctgaaagcg atgccggctg gtcttgagcc gcgctttgcg ttcgcgaccc
    10201 tgcatcaaaa cggcggggac attgcccgtg ccccgctgaa tgccctgcgc ggattgccgc
    10261 tcagccgatc cgggccgcga gatggcctcc aaggcgtttc cccagggcca ctaccgtcca
    10321 catcaccggc aaccccagcg atgaaggcag gacgcttgcg tcgcagcagt aaagatgatc
    10381 gatctccgtt tcaaggttgc ggtccaccac atcgccgatt ctgcaggttg ccgacggatg
    10441 agctccctgc ggattgagag gaaggatcgt cgaatctttc gcgccggcct tgcgaaacac
    10501 ctttttgatg atgtcaactc ccttatcgag cttcatccgg tccgcctcgc tgaccttctt
    10561 ggaaaaggat acaccctgaa aaagctcgcc cgccgtttca tcccgcactt tcaccatgat
    10621 gcccgagagc ttccagaatc ggggaaagtt cagcaaactc ttcggccccg ccattgcaag
    10681 cgtcgccgca tacatggacc agttcggaaa taccgggata atggcgatgc cgtcggattc
    10741 atagaactca agggaaccga cggacatggg cgtcgcgccc acggtattca tgccgggaat
    10801 ctccgccccc acgaattgaa gccagtcgca gcagaaaccc tttccggcgc gagaaatccc
    10861 ggcacggcgg agtatatgag cattgtcgat ccccgtggag agcacaacga attttccgaa
    10921 atatttgacg gggacacccc gtcgcgtgcc ctttacgccg acgacccgat tgttttcaac
    10981 gataacttcc gtgatcctgg tatggagatg gaggtccgcg ccctgactga ccgcctcgtc
    11041 gccatagacg cgagcggtcc acttggcgcc ggtcctgcag cccagcatgc agtccccgct
    11101 ggccctgcac cgcttcggat cgacgaattt gtccattttt ccccagtggt accccaggct
    11161 gttggcgact tccacgaggc gcagattctc cctgcccaca agctcgtcag gctgcttttg
    11221 tatccacaga tccttcttcg cctccccggc ctcctcggca aggtcgattc ccaccggatc
    11281 gaaaacactt ttgggcggag gcgccgcgca tccgcccgcc aggttcgaca aaccgccgta
    11341 attgtcggca aaggtaaccg tgcggccttc cctgctgcgc accagcccgc aatgctttag
    11401 aatcatggcg atggtcatgg tgctgcccat ccattcccat cgcccgcccg cctcgagcag
    11461 caagactttt tttccgcgac gggtcatttc ccgcgcgacg gttgaaccgc ccgtcccggt
    11521 cccggcgacg atcacatcat attctttttg catcactcct cgctgatgaa tcacggattt
    11581 tttgaacaga cgcatattca tttttcacga tatccatatc caggggaacc gtcttttggg
    11641 ggtcccgata gatgcggatc gcctgctcgg gacaatgctc catgcacaga ccgcatccca
    11701 ggcagatttc tttatgatag gtttgttgat ccccgctctg cacaatcgct tcgaactggc
    11761 agattcgggc gcaggcgccg cattgccggc agagagcctc atcccggtgc atggagtagc
    11821 ccgagtcggc gttcatcgtc aggcccggat cgaagcgccg ggcgaagcgg gtcgcctgaa
    11881 ggctcacgca ggtgtcgatg tgacagttgc agataatccc cgtgctgccc cccgtcgcca
    11941 ccttgaagaa ggcctgcgtg acataccctt tttccctgaa ctttttgacg aggtcgacgg
    12001 cctctttttg agaaattttc cttgcatgat atttcgctcc gcaacggtcc agccagaact
    12061 gcgaggtctt tttccccacg ctgatgcagg agtttatggt ccattccggc gcgttcagag
    12121 attttttgca gggacaatcc atgacggcaa tgaaatccgg ctcctgaaga agaattttgt
    12181 acgcatgatc gaagggaacg atctgcttgt ttttttcaga aaccgccgtg acgtcttcat
    12241 cgagttcaag aatttttacg gcatcttctc ccgatataac cttggcatgg taacgggcga
    12301 aagccgccct gatcagcagg ttgaggggtt taaaccagga cagatgcctg gccaggaagg
    12361 caaaaaaatg atagacaacc cttacgtaag gataataaaa cgagaaataa agatagttgt
    12421 ggatggcccg gtcgatccgc cagccgtgct tcgccatcag agctttcgtt gatgcccgca
    12481 tgcctgctcc ccttcggaaa tcattttata aaagattgga aatcccatca agagaagtct
    12541 cccgcagcat cggccgcttc ctttggtttg cctcgccaaa agaagttttt ttcaatactc
    12601 ccgaatgttc ttccggtgat tgaagacaat cagcgcaaag atcgcggcgg cgccggccgc
    12661 ggcaagccag tctccgctgg aagaaacgac gtgccccgcc gaaagcgcgg ccgtcatgaa
    12721 aaaagaagcg ataaatggcg cccgcttcag ccagtaagcc agaacccaga gccacgcggc
    12781 cgaaagcgcg gcgacgggaa cggtcagaac cgtaaacccc aggtagttgg ccacgccctt
    12841 gccgccccgg aatccgtgaa agcagggaaa gctgttgccg agaaccagaa aaaacccggc
    12901 ccagatcagc cacccgccat caagaaaata agcggccagt gccgccacgg ccatcgcccg
    12961 gccgatgtcc agggcaaaaa ccagcaccgc ccagggcatg cccgccagac gatagacatt
    13021 cgtcgttccg gcattgccgc tgaaactcag gcgcggatcg gcgcggcctg caattttgaa
    13081 aaacagaatc gcaaaattga cggagccggc cagatacgcc gcgcaacaaa tccaaatgac
    13141 gttgatcatt ccccttcccc gatccggcgc ggcgtcttca ggcgcccccg gaaaatatcg
    13201 ctcattctca aaccgcgaat gctttcacaa agcatttccc tccggtcaaa gatccacacg
    13261 tcaaacacca ggaatccgcg gtttccattc accgggatca ccctggcaaa gcaggtttcg
    13321 ccgtttttca ccggccggta aatgattctg tggtcaaaac cggtcggaaa ggccgcaatg
    13381 ccggcaaacc gctgtcccca gacgcaggcg gcctgcattg ccgcatccgg cgcaaaagga
    13441 gatcccagcg gcgtgtcgtc cgcttcgccg ctccctcctg aaatttccgc ggacacgcct
    13501 tcccgcgaaa cggccagagt tccgacaaga ttccggtagg acggaccaaa gggaatccat
    13561 tttcggtaaa ccgaatccgc cgcgactgga atgctgtcgt ctcccgtcgt tcctgacgcc
    13621 tgaaaaggca tgggcgcttc gggcggagca tccttccgga caaaggtcac ccgcgcatgc
    13681 accagcgccc ttcgaatgga ggcggttttg gcgctcaccg tcgtctgcag ggacgcatga
    13741 atcccgttct ccctttcctc aatttcaatc cgcacaggca gctcatccgc ctcctccggc
    13801 agcgccagca ttttgggaaa atccgcgtcc gccaggcggt tcacaagagc gccggggcgg
    13861 cggttttgca cctcgcgggc caggagcgcg agggactcca cggcggggaa aacggcctgc
    13921 ccctccacgc aatggtcgcg caggtacggc gggatcggaa gcttgaagga aaagatctgt
    13981 ttcataccgg atcgggaaag gtctgccaca ggcgaatcgt cttcggatcc acggccacgg
    14041 ggcggccggc ggcgttggcc gcgcagtgtc tggtgaatcc cgtgcagacg aggcgccccg
    14101 catccgcgcg ggtgacgaca taatcgaact ggagcctcac gcgctccagg ttgaccggcc
    14161 gggtgtggat gtgcatgacg tcgtcgtaaa ccagaggctg ataaaacgaa atccccagat
    14221 cgtaaatcgg atacacatac ccgctttctt caatttccct gtagggatag gccaggtccc
    14281 gcatcagcga ggtgcgcccg acttcgaaat agcgcagata gttggagtgg taaacgacct
    14341 gcgagcggtc cgtgtccgca tacagcaccc ggtacggggt ccggtgccag accagtcccg
    14401 tggtccggtc gcggacgaat cgttcatcca tcttgcaggt ttcaggcaaa aacggcttgg
    14461 gtttcatatc acactccccg gaagatcacg cagcagttcg tcccgccgaa tccaaacgca
    14521 ttggacagga cgatttcatg ggtttgtttg cgggccgtgt tcggcacgac atcgatatcg
    14581 gcaaattccg gatccggcag gtggttgatg gtcggcagca gaatcccccg ccgcatgcct
    14641 tccacggcca gggccgcttc aatggcggcc gtcgctccca gcgtgtggcc aagctgggat
    14701 ttgttggagg agacgggaat catttccatc aggccgccga agacttcgcg cagacattga
    14761 atttccgtcc ggtcgccttt ggcggtggag gtgccgtgcg cgttgacata ctggacatca
    14821 gcaggctgga gccccgcgtc cgcgatggtt tcccgcaggg cccggacaat ggttgccgga
    14881 ttggggctgg tgtaatggta ggcgtcggac gtccagccca cgcccagcac ctccgcccgc
    14941 ggttcaagcc cgtgcgcccg gataacctcc tcggcggcca gcacgacgac gcccgctcct
    15001 tccgaaagga caaatccacg gcggtcgatg ctgaacggac gggaggcctg cgcgggatcc
    15061 gcggccgcgc ggtcgccggc gtgcaccttg atcgtagcgt tcatgttggt aaagccgtgg
    15121 atgagctccg gaagaatcgg cgcatccgcc ccgcccgcca gaacaaaatc gcagtccccg
    15181 tcgcggatca tgcgcgcgcc gatgccgatg gcatggttgc ccgaggcgca ggcgccctgc
    15241 ggcgaaaaga tgggtccggt gaatttcagg agcatacccg cctttcccga cggcagattg
    15301 gcgcacaggt tcggcagcag gtaaggactt acccgcaaag gcccgcgatg ttccaggtcg
    15361 tgcatggccg cgcgaaacgc atccgtcccg ttgagcgccg atccgatcag acaggccatt
    15421 cggggggcaa tggtttcatc catcacgatt cccgcgtttt gaagcgcttc cttgcagacc
    15481 gccatggtca ggagaacgaa cgccgcgttc cagttataga cttccctggc gtcggagaaa
    15541 tccaggttcc ggggagacca gtccggaatc tctcccacga tgtcgcaggc cgattccacc
    15601 ttgcagcggg tgaccttgcg gaatcccgca tcgccctgaa cggcgcgctc ccaggttttg
    15661 gcaaacgtgc tccccagcgg cgtggccgct ccatacccga cgataaagac tcttcggttt
    15721 tgcggaggtt tcatcctttg cctttcaggg ccttatgcga tccggcgcaa aagcaggcag
    15781 gaattgcacc cgccgaagcc gaacgcgttt ttgagcacaa actcctgaca gagttttctg
    15841 gcggtcccgg gcacacagtc gagatgaatg gccgggtccg gttcgtagtt gacggttggc
    15901 aggactgtgt cgcgcagcat gccctcgatc gccagaatcg tttcaatggc ggaagacgcg
    15961 cccatggcgt ggccgagctg cgacttgttg gccgaagtgg gcggcgcctt tgcgccgaag
    16021 atttgagtga gcgcgtccgc ttccaccttg tcgccgattc tggtcgatgt cgcatgggcg
    16081 ttgacggcgt ctacctccgc ggacgtgagt cccgcatccg cgattgcgac ggctatgcag
    16141 cgctcgaccg tcgccggatt aggcgccaca aaatggccgg cgtcggacgt catgccgaac
    16201 ccggcgattt cgatgcgcgc cgcaagcccg tgcgcccgcg caaaatccgg tgacgccaga
    16261 atgaccgcgc ccgccccctc ggaaacgaca aatccccggc ggttggccga aaacggccgg
    16321 cttactttct ccggcgcttc gtggggccgg ccctctttcg gacgatacgc gccgttcatc
    16381 gtcgcaaagc cggccaggat cggctcgacc agcggaaaat cgacggcccc ggcgatcacc
    16441 acgtccgcca gattctgttg aatcatcatg ccgccgatga tcatggacgt gacgccggtg
    16501 gcgcacgccg taatcgtggc gcagatcgga ccggtggcgc ccgtcaggat ggacaccttg
    16561 ccgcccacca tgttgatgca ggaattggga ttggcaaaag gatgcggcag cttgttttcg
    16621 gcgatcatct ggcggtccgc cttcagcacc gcgtcctgtc cgccgacggc cgaactgaag
    16681 gtgacggcca cgcgcggcgc gacatcgggc gtgatgtcga tgccgctctt cgccagcgcc
    16741 cggtggacga ccaggagcgc gtatttgaag atcggcgacg tccagtgcgc catttcgcgc
    16801 ggctgcaaaa aaggataggg ccggacatcc acgtcctcga cctcgcccgc gacgcgaacg
    16861 ggaaaatcat cgccgtaggg gaaacgcgtc agagggccga ttccgctttc gccggcggcg
    16921 gcccgccgcc actggatttc cagatccgtc cccagagacg aaacggcgtc atacccgaga
    16981 atgagtgttt ttccggacaa gcgcaaactt tccctttcca gagatttctt tttacgagac
    17041 gacatcatgc aaaagctttt caaaaatatc aatgacaatt ttattccccg cggaaaaccc
    17101 gcagtccgca aaaacaaagc ctgcggccgc ggccgccgct gaagcttcag ccggtttgtc
    17161 ttgacaatct tcaacggatt gcgataaggt ggcgccgttt ttaaattgcc gttcaccgtc
    17221 gccgcctgat accccttcac cgccccttcc ctttcccggg ggaagcaggg ccaaccaccc
    17281 ggaggtcttg gatggaatcc attatctgca aacactggca ccactgcccc ggaagcgaag
    17341 tgaccacctt gctcaacacc gatccggaaa aaggcctgtc cccttttgaa gccggacacc
    17401 ggctgaaaaa atacggcccc aacgccgtca ccgtaaaaaa gcaacaggga ccgctgatgc
    17461 ggtttctgct gcagtttcat cagccgctga tttacattct gatggccgcc ggggccgtca
    17521 ccctgttcct tcaggaatgg gtggattcgt cggtcattct gggcgtggtg atcatcaatg
    17581 ccgtcatcgg ttttgtgcag gaatccaagg cccagagcgc cattgagtcg ctcaaaaaaa
    17641 tgatgacgac gcaaaccacc gtgctgcgcg acggccggtg gcaggaaatg gacgcggtga
    17701 atctggtgat cggcgacatc gtccagattc agtccggcaa caaggtgccc gccgatgtcc
    17761 gaatcctgcg ctgtcgggat ctgcaggtcg atgaatccgc cctcactggg gaatccgtgc
    17821 ccgtcatcaa aaaagaagag acccttgatc cggaaaccat cctggccgac cgccgcaata
    17881 tggcctatgc cggcacgctg gtcacgtacg gacaggcgcg ggcggttgtc gtggcaaccg
    17941 gcgacaagac ggagaccggc cgcatttccg agctgatctc ctcggccgcc gatctttcca
    18001 cccccttaac gataaaaatc gcctcgttca gcaaaattct tctgatcgcg attctggttc
    18061 tcgcggcggc gacgtttgcc gtgggcatgt ggcgcggtga aagcgccgtg gatatgttta
    18121 tggcggcggt cgccctggcc gtcggagcca ttcccgaagg actgccggcc gccgtaacga
    18181 ttacgctggc catcggcgtc aaccgaatgg cgaagaagag ggccatcatc cgcaagctgc
    18241 ccgccgtgga aaccctgggc agcactacgg tcatctgctc ggataaaacc ggaacgctga
    18301 cggaaaacca gatgaccgta aaaaagattt tcacgggcgg ggcttcctac gacgtggaag
    18361 gaaacggtta cgcgccggaa ggccatcttt cggcgaacgg gaaggaatta accggcgatt
    18421 tgcccccggg attgcgccag acgctgacgg cggggcttct gtgcaacgat tctcgcctga
    18481 ttcaggaaga agatgcgtgg cgcatcgaag gcgaccccac ggaaggtgct ttaatcaccg
    18541 cggcgggaaa ggccggcctg acaccggaga aaaccggcgc cgacatgccc cgtcttgacg
    18601 aaatcccctt tgaatcggag cggcagttca tggccacgct gcaccgttcc acagcaggcg
    18661 gcaaaaacat cctctacatc aaaggcgcgg tggaaaaaat tctggaggcc tgcgccgacg
    18721 ccatggcaag cgagggcggt aaaaccgctt tcgacaaaga gaacctgccg gcccgggtgg
    18781 acgccatggc aagcgagggc ctgcgcgtgc tggcgctggc ctgcaaagag acggacaagg
    18841 atcatctgga ggttgaagat tgcctgtccg gtctgacgtt tctggggctc cagggaatga
    18901 tcgatcctcc ccgaaaggaa gccatcgagg ccgtccgatc ctgccgtcgg gccggaatcg
    18961 ccgtcaagat gattacggga gaccatgcgg tcacggcaac ggaaatcgcc cggcagctga
    19021 acatcggaag cctgaagggc aaagacgagg ccgcaccgcg aaccctgacc ggcaaagaac
    19081 tggaaaagct gacggatgaa gaactcatcg cggcggcgga ggacacctcc gtttttgccc
    19141 gcgtcgctcc ggaacagaaa ctgcggctgg tggaagcgct tcaggcgcgc agccatatcg
    19201 tcgccatgac cggcgacggc gtcaatgacg ctcccgctct caagcaggca aacatcggca
    19261 tcgccatggg gattaccggc acggacgttt ccaaggaagc cgccgacatg gtgctgacgg
    19321 acgataattt tgccagcatt acgcgcgcgg tggaagaagg ccgtcggatt ttcgacaacc
    19381 tggtcaagtt cattacctgg acgctgccga ccaacggcgg cgaaggtctg gtcatcctgg
    19441 cagccattgt aaccggagtc acgctgccga ttctgcccgt gcagattctc tggatcaaca
    19501 tgaccacagc cgtgctcttg ggtctgatgc tggctttcga accgggcgaa tcggatgtaa
    19561 tgaattaccc gccgcgcgat ccccagagcc ccattctcaa cgcgacggtc attaccagaa
    19621 ttctgctggt aacggccatc atgctcgccg ccgtcttcgg catattcgtc tggcagaagc
    19681 agcagggcta ttctctggcg gaagcccgca ccgtggcggt gaatcttttc gtcatgattg
    19741 aactggccta tctgtttaac tgccggtcgt tgaccaaatc gatgttccgg atcggctttt
    19801 tttccaatcc ctgggttttg gcgggcagcg ccgcgatgat cgttcttcag ctgctctaca
    19861 cctacacccc ggccatgaac cgcgtttttc agagcgagcc gatttccggc cgggactggc
    19921 tgatgatcat tgcgatttcc attatcgttt acgtcgtggt cggcgcggaa aaatggctgc
    19981 ggcgcggcgc ttttgccaaa acgcactgag aacggaccgg cgctgggcga aagcaaataa
    20041 atatcagaaa attcaccgta tggaggttga accatggatg cacaaaaaat tccgggaaag
    20101 gtgatcatcg gcgccgtcat tggttttgcc gccatccttc ttttgctgtc cacgtatttc
    20161 agcatcgagg cgggcgaccg cggcatcgtg ctgcgcttcg ggcaggtcaa ccgggtggtt
    20221 gagccgggag cacgattcaa aatccccttt gccgaggagg tggtccgcat gagcgtccgg
    20281 gtgcagaaga ccaccaccaa gaccgaagcg gcaagccgcg atctgcaaac cgttcaaacg
    20341 acgatggtgc tcaactacag cctggacccg gcccgaaccg gcgagatgta cgccaacatc
    20401 ggcctgaatt tcaatgaacg cgtcatcgac ccggccgtca aggaaagctt taaggcggcc
    20461 gcggcccgtt acacggcgga ggagctgatc tccaagcggg aaaccctcaa aacggaagtg
    20521 cgcaattatc tgcgggagag gctgcacgtc ttcgggatca acatcgtcga gctttccgtg
    20581 acggattttg aattctcggc ggaattcaac cgggcgatcg aatccaagca gaccgccgag
    20641 caaaacgcgc tcagggccaa gcgcgatttg gaaagaatca aggtggaagc cgaacagaag
    20701 gtcacctcgg ccaaggccga agccgaagcg ctccggctgc agcggcaggt gatctcgccg
    20761 gagctgatcc agctgcgcca gatcgaagcc cagatcaaag ccattgaaaa atgggacgga
    20821 aaactgccga acgtcacggg cggcgcggtc cccttcattc aggtagaaaa gcccaagtag
    20881 ggaacggctt cagcccgttc cccgcagttt tttaccacgg aataaactta tagagtttgg
    20941 cgaacatttc atagacttcg atccagttgt gcagatcccg ggtcgcgttc atgtggtcgc
    21001 gcttgttgac catgacccag ctcatgtgcg ccgcgccgtc cttgcaggtg gagcagtcgc
    21061 gcgtggcgga cgtgcagcag cgatggacgg tctgcaggtc cgccgcccag cggtaaaaat
    21121 gaatgagccg gcgcaccggc ggctgccgat ggtccaggga ttcggtcacg gacggacatt
    21181 cccgccagcc gaaacggcgg ccctgcatca ggccggtggt gatgatttcg tggtaatacc
    21241 ggcaggagat gacggttcgc ggataatgat ccagcatttc gtccagttca aaccgcagat
    21301 cgctgagctc ttcgggccgc caggaaaatc cgtccacgcc ttcgtcgttg gatagcagct
    21361 gcaggtgaac tttcaggccg gcatctcgaa tgcgccgaat gaccggttcg gtttttccca
    21421 cctgtcttgg cgtcagcgtg taaagatagt aggcgcaggg atcgccctcg tagttcttgc
    21481 tggagacggc aaacgtgtcc ctgccccgca gaattttttc atcttcgccg ccgccccaca
    21541 gggaaatgcc caccatcata tcgggaaaac ggtcacgggg aaccttgatg aggccgttgg
    21601 tcgcgcagta cgtcggaacg cgccggtaaa aggcttccag acggtccatg cacagcgtcg
    21661 gctcgccgcc gatgagaatc gccagattca cgccgcggat cttttcccgg tcgataaagg
    21721 cctcccacct gcgaatatcc atttcttcct gaacgtggtg ctcacctgag gaaaagaaga
    21781 aacatccctt gcagcgcaga ttgcagcggt ttgtgagatc ataaatcgag ctgcggatgt
    21841 tgaggctgga tatccggcga tagcgttcgc gccattcggc atccagcagt gtgcttaccg
    21901 tcttcattct tcaggacctt ctccctttcc attcccgaga cacgccgcaa aggctaaccg
    21961 gcggctgctc gcggaggatg gatgatcttg ttgcagagat tctcgccttt ttcatatccc
    22021 atgacccaga ccgccagata cgtgtccacg tagtccagcc aggacttgaa agcgtcctcg
    22081 tcgccgacat gacggtagag ccttgccgtc acgacggcgg aaccggcggc gtaatggcgg
    22141 cagtcggcgc aatccgtatc cggcacgcag caggccgccg accggtccca gttcaaatcc
    22201 gtccgg
//
LOCUS       DAOSIM010000008        21958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_2629_length_21958_cov_9.066566, whole genome shotgun sequence.
ACCESSION   DAOSIM010000008 DAOSIM010000000
VERSION     DAOSIM010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 21958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21958
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2629_length_21958_cov_9.066566"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLR47_00800"
     CDS             <1..406
                     /locus_tag="PLR47_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG11499.1"
                     /translation="GIGIGCEKVGHIFIDRSNHEKALASLRAAKEKIVNGTSVIFFPE
                     GTRSRDGSLGVFKKGAFKMAVDLRLPILPITIVGTRDILPTDSVDLFPGRARMIIHKP
                     IDTGGYKDDNLDELVDRARGIIGSGLWKGTPE"
     gene            complement(446..1807)
                     /locus_tag="PLR47_00805"
     CDS             complement(446..1807)
                     /locus_tag="PLR47_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG11500.1"
                     /translation="MRKLNILIVEDGQSQREMLRDFLISEGHAVTEAENGEAAVSAVS
                     NRHFDLLLLDYKMPGMSGLEVLEEVKKINHEIDVVIITAYGTIETAVEAIKAGAVDYI
                     TKPIELDELLILVDRVAERRGLIRENDLLKQELGKTGVTAEKIIYKSSRMVELINLAG
                     RVAASRASVLIQGESGTGKELLARLIHQLSPRASRPIVVVNCGALQENLLESELFGHE
                     KGAYTGADARRIGRFEEADGGTLFLDEIGELSPAIQVKLLRFLQEREISRLGSNASIS
                     VDVRIISATNQDLDARVSDGAFREDLFYRLNVVAVSLPPLRERKEDLTALIDHYLEKF
                     TRENGKNIQGLTAEARDLLLKYDYPGNIRELVNIMERAVVIAREDYITVGDLPFKNES
                     FNNFSGRNISGSLLEMREELEKNLIIEAMAKEDNNQTRAAASLGMTRRMLRYKLKKYD
                     LKN"
     gene            complement(1825..3627)
                     /locus_tag="PLR47_00810"
     CDS             complement(1825..3627)
                     /locus_tag="PLR47_00810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG11501.1"
                     /translation="MRIFQKKETIVQRGKRPYKGKFLQPLAVGIVSAVLVALILVMGY
                     LDIRRNETNLVGFMEDQALTTISVLLRQTEDNLKSIAAAKGKMPSHLKTAKEAESAYS
                     IQLVYDAITKLGQQIDEQWKKKLMSNAYLQKFAEDHYFWYVAILNMRGDILYQSGPLK
                     TDMLSKSDLFINGRKATTVELLEKIRIKEDIGYVGVKLKRGGGIAVFCLDRKGRLYWS
                     LKVAVERAMSKMGEGHGIAYLQVFNDQKILLGNIGELPAGLTSRDLNDQEMMKGKKGI
                     LSRKVDPQGIKILDVAAPFILENPYLPKEKIAGIVRIGLDRGSMDKIIAENRQNIFIF
                     MLFVAGIALLSMWLLYHDQNRHLAGIIEMERRLEKAERLSSLGQLAAGVAHEIRNPLN
                     AISMATQRLKRDYVPADKLKAHEFNNLSTVIRDEIRRLNAIIEEFLSFSKSRRLELRD
                     FSVTEVLQKIVSLLAEEAGSRGIALETKWQLSPAVIPMDINKLQQAFLNFIKNAMESI
                     SKKGVITITVVREGKNNILVSITDTGCGMTSEEIEKIFSPEYTTKEKGLGLGIPLATE
                     IVRGHGGDVRVVSRLGEGTTFEIVLPIKKKCEKT"
     gene            complement(4086..>5133)
                     /locus_tag="PLR47_00815"
     CDS             complement(4086..>5133)
                     /locus_tag="PLR47_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOG11502.1"
                     /translation="WEAKKQKTILFVDEIHRFNKAQQDAFLPHVESGLVTLIGATTEN
                     PSFEVIAPLLSRTRVMLLKQFAEEDLTGILKRALSDPQRGLGQFAIQIDDEAIKFIVG
                     ISDGDARTALNNLEAAASMVQSLPEEERMISAKSAEEALLKKSLLYDKDGEEHYNLIS
                     ALHKSMRGSDPDATLYWLGRMLAAGEDPLYILRRMVRFASEDVGNADPHALVLTMAAQ
                     QAFHFIGLPEGELAIAQAAVYLAVAPKSNSLYTGYGKTKDLIHKTGYLPVPLHIRNAP
                     TKLMKELEYGKDYKYAHDYQDAFVPQEYFPEKLQGKVLYSPTDAGYEKIIRERVEEWR
                     RRKNKAKKESEKKG"
     assembly_gap    5134..5143
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5144..5425)
                     /locus_tag="PLR47_00820"
     CDS             complement(<5144..5425)
                     /locus_tag="PLR47_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG11503.1"
                     /translation="MKNLDLFSQGTPADALETAPLAERMRPLTLADYIGQPHLVGEGT
                     LLRRSIAEDKLFSMIFWGPPGSGKTTLARILANETKANFVSFSAVLSGVK"
     gene            complement(5422..5679)
                     /locus_tag="PLR47_00825"
     CDS             complement(5422..5679)
                     /locus_tag="PLR47_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11504.1"
                     /translation="MKTPHDHKITRCPKLGDEMTFAYCLREAGNLPCARILFCWQAAF
                     DAASLLQENLTQEELKRFAEILPKDKVVSLIEILEKTKRQT"
     gene            complement(5782..7146)
                     /locus_tag="PLR47_00830"
     CDS             complement(5782..7146)
                     /locus_tag="PLR47_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOG11505.1"
                     /translation="MEQVKTLLEDQEIPRQWYNIMADLPTPMKPPLHPGTGQPVTPDD
                     LAAIFPMNIIEHEVSTQRWIDIPDEVLQKYLLWRPSPLCRARNFEKLLGCPVKIYYKN
                     EGVSPAGSHKPNSALPMAYYNKVAGTKRITTETGAGQWGSALAMACQMFGLECRVYMV
                     KISYGQKPYRRLMMNTWGAECIPSPSTLTQSGRDFLAKYPDSPGSLGVAISEAIEDAV
                     TSKVKSHYALGSVLNHVLIYQSIIGLEAQKQMQKLGIYPDVVMGCCGGGSNFAGIAAP
                     YVCDKIHGKQVKIVGAEPSSCPSMTKGPFAYDFGDLAQKTPLLPMYTLGHDYVPAPIH
                     AGGLRYHGMAPIVSHLVREKFIEPRAYDQLKTFDAGVKWARSEGFIPAPETNHVLAAV
                     VDEAMRAKEEGKEKVILFNWSGHGLVDMASYDAYLSGKLEAYELPDEEIQRALLAIKD
                     FPKP"
     gene            7602..8057
                     /locus_tag="PLR47_00835"
     CDS             7602..8057
                     /locus_tag="PLR47_00835"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOG11506.1"
                     /translation="MTQSEFKKIIANAIKAEVAANKFYSGVAKKAKDANLKKLFKELA
                     DEEAQHRLTLKGYAARGAEKFTFTESADYKVADELPTPPLTADLKPVDGLVIAIKNEL
                     AAMQMYTQLAKASADKAQKKVFQELASMERGHKKKLEDLYTNMAFPEVW"
     gene            complement(8158..8661)
                     /locus_tag="PLR47_00840"
     CDS             complement(8158..8661)
                     /locus_tag="PLR47_00840"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOG11507.1"
                     /translation="MRKVWLTVLGGLLSVVFCMSVLAQTPPLSDGAQDSLIKSQVETA
                     VSLLAAIHARQQKGEMSADKAKELAAGLLRELRYGKDGYFWADTTEGVNVVLYGRKDT
                     EGRNRLNDRDAKGVFYVKEFLAKGKTGGYVEYRFTKQGQAASLPKRSYVLLFKPFGWV
                     VGTGYYR"
     gene            8833..10407
                     /locus_tag="PLR47_00845"
     CDS             8833..10407
                     /locus_tag="PLR47_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG11508.1"
                     /translation="MNQPSSPVEIDFNSEFQRAISLMEDTQKHILITGRAGTGKSTLL
                     KYFRDTTSKKAVILAPTGVAAVNVEGQTIHSFFHFKPNVTPASIKRRKKAEKDRLTLY
                     QKLTTIVIDEISMVRADLLDCVDKFLRFNGPSEKKPFGGVQMIFIGDLYQLPPVVSSA
                     EREIFKNHYPTPYFFSAKVFEALDIEYVELEKVYRQKDDEFVRLLNTIRNRSVTDDDL
                     AKFNSRCDPFFEAPSGSFYLALTSTNDLADTINEKRLAELPGKIWKAKGQIEGDFGKE
                     YLPTAVDLKLKKNAQIMMLNNDSFGQWINGTIGKIRKFEKDDEGDDVIVAELDNGDTA
                     RISPYTWKIYRFFIKDEELRSEEVGSFRQYPVRLAFAVTIHKSQGKTFENVVIDVGRG
                     TFAHGQMYVALSRCTTLDGIVLKKPLQKSHILMDWRVVKFLTNLQYARAAKTLSREDK
                     IKWIEEAIAEKKDIEILYLKSQDEKSRRTVRPLFVGKMEYKGYPYLGLEAICLERREK
                     RIFNVDKILEIGAEKR"
     gene            10456..12906
                     /locus_tag="PLR47_00850"
     CDS             10456..12906
                     /locus_tag="PLR47_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG11509.1"
                     /translation="MAKQIFIVDDNPNNLYLLASILKSRGWSVREAGNGKDALDAIRV
                     QPPDLIVSDILMPVMDGYALCRECKADPKLKSIPFVFYTATYTEPKDEDFALSLGADR
                     FLLKPQEPEILTGILEEFLHEGKAAPPEASSPLGEDMEFFRRHNEALFRKLEKKMQDL
                     EAANRELRMLEEIYRQSFEHVTDVIFTVDAGLRIISISPSVEKALGYKPREFIGRSIR
                     DLGYLFAADSMETALEHIGLVLQGTALPATIYEFRARDGAAKFGEVSGSPITRDGKVA
                     GLVAVARDITERRLAEQKLRENEKRYKLLTEKMTDIVWISDMNLKTLYVTPSVQSVLG
                     FSQEERMHQAVEQQITPESLSRGLEAMAAELALEKQALGDPTRLATLLLEYYHKDGST
                     RWMETIISGLRDDQGVLTGLHGVSRDVTKRKKAEEEKQKLEERLHRAEKMEALGKLAG
                     GVAHDLNNVLGVLSGYSELLLLELPEDHKARKNVEKILQSTEKGAVIIQDLLTLARRG
                     VAVSGIVNLNRIVDGFLKTPFFEKIKNDHPRITFRAECDPRLLNIKGSAVHLEKALIN
                     LVSNAAEAIAEAGEIVIRTENRYVDRILISHEEVQEGDYVLLIVSDTGMGIPAEHREK
                     IFEPFFTKKTMGWSGTGLGLPIVWGTVKDQNGYIDLQTKEGKGTTFILYFPVTREEAA
                     LPQQRKPLEDYRGGGERILVVDDIAEQREIASSLLMKLGYEVETASGGEEAVKFLQEK
                     EVDLVLLDMIMSPGPDGLETYRKIRDIDPRQKTILVSGFSETERVLTAQRLGAGAYVK
                     KPYVMETIGLAIRHELKR"
     gene            complement(12943..13170)
                     /locus_tag="PLR47_00855"
     CDS             complement(12943..13170)
                     /locus_tag="PLR47_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11510.1"
                     /translation="MRAIISVSGWPCGGNGHRSVALSRKSFAAAMRKTPKTGRGPTMA
                     EIDRLISAAGAVALILSAFYFAPIVGSIFMK"
     gene            complement(13199..13786)
                     /gene="lexA"
                     /locus_tag="PLR47_00860"
     CDS             complement(13199..13786)
                     /gene="lexA"
                     /locus_tag="PLR47_00860"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOG11511.1"
                     /translation="MKKVRDIKSVEQSLVSFYRRQKRMPTYAEMMDLFEVRSKSVVSY
                     WIEKLIEKGILDKDAQGFLKLSGVSFGIPLVGNVAAGIPASAEEIPRDVLSMDEYLVT
                     RPESSFLLRVTGDSMIGAGIMEGDLVVVERERTPRNGDIVLAEVDGQWTMKYFRRRGG
                     KVVLEAANPAYAAICPKEELKIGGVITASVRKYLM"
     gene            complement(13907..15634)
                     /locus_tag="PLR47_00865"
     CDS             complement(13907..15634)
                     /locus_tag="PLR47_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HOG11512.1"
                     /translation="MKTNASFSRFDRGVELHSLDRFGLRIKDARPSAARIFHFVLVLL
                     LLAAAACDRTPQTGSAGSKGDKPPAYGDILVRGDIGDASNLIPLLASDSASHAVGGMV
                     FNGLVKYDKNMNIVGDLAESWEISPDGLSITFHLRKGVRWHDGRPFTAADVLYTYQVT
                     VDPKTPTAYAGDFQKVKKALVLDDHTFRVTYDKPFAPALISWSSAVMPRHLLAGKDIA
                     QSPLSRHPVGTGPYRFKEWVPGQKIVLASNEDYFEGRPCLDGRITRIIPDTATMFLEL
                     RAQNIGMMGLTPLQYTRQTENNLFRQNFNKYRYLAFAYTYLGYNLKNPLFADRRVRQA
                     ISHAINKDEIISGVLLGLGKPATGPYKPGTWACNDKVKIYHYDPAKARELLKQAGWAD
                     ANSDGVLDRDGRSFEFEILTNQGNETRQKCAEIIQRQLKEVGIAAKIRVLEWSAFVTH
                     FINKRRFDAVILGWTIPPDPDAYDVWHSGKTAPEELNFVSYGNPEADEMLEKGRSTFN
                     QAERKKYYDRFQEILAEDQPYTFLYVPDDLIIISNRVRGIEPAPAGIGHNFIRWYVPK
                     DEQKYTMTR"
     gene            15814..16461
                     /locus_tag="PLR47_00870"
     CDS             15814..16461
                     /locus_tag="PLR47_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOG11513.1"
                     /translation="MKVKTQELFNFYSHFAGAVAAVVGTVYLAMAASDSASGLVTALI
                     YGISVVFLFSASTLYHAFKKEENELSFWRKMDRLAIFFMIAGTFTPICYFCLEDPYRW
                     WMIAFQWGLVGVGVISQIFFPGAPRKIYAIIYLSMGWSALFTIKQMLSNMSSSQAVLL
                     VSGGIAFTLGAVIYAVKKPRMIPGVFSFHELFHIMVLIGGVLHYAMIYGVYSRPV"
     gene            16596..17363
                     /locus_tag="PLR47_00875"
     CDS             16596..17363
                     /locus_tag="PLR47_00875"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOG11514.1"
                     /translation="MPFFLRAPGSDTGVLLIHGLMAAPAEVREWAEFLHARGMTVYAP
                     RLAGHGTSAKDLLVRDYSDWLNSVDRGHAILKTCCRRILVAGFSTGAGLALQSAIGKP
                     HDFEAVIAVSAPLRFKSFSSRFAELLHGFNLCCSHVGLNRLAVEFVKNDADNPHINYL
                     LCPVSSFVQVKKLMRSVRRRLPDIQIPALILQGRHDPKVAPESGPAIFQRLGAHQKRF
                     AWIDFNRHGIVLGPAAREVFHEVESFLNDCRLTGSSK"
     gene            17487..19019
                     /locus_tag="PLR47_00880"
     CDS             17487..19019
                     /locus_tag="PLR47_00880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOG11515.1"
                     /translation="MTEKKEDSRARRKTRKESMQMKAAGAWDIAFDAVNNFSLNGDAN
                     QAAAIALYAILSAIPLFILTLIAAGSFFSSNTTLQTDIIGAVQGVHPYFSEALLQQVG
                     QIEGKSKVLGWIGLLGLVWLSAAIFNAIESALNIIFRSRRKRNYFVSKLMAVAMIPAA
                     WLIGGLSVVITYLAALLAKEPSGWAGGVLFSLTMPAQIALRYLIPFLMVVLLVTFVYR
                     IIPTAKIRFSVVLAGSAIFAFLVEIAKQLFTWYLANYTRYNVIFGSLETVVILVIWVF
                     YVALIFLFCAEIMSSFERRDMLLLEKALLKPHDSLLKVDARLFQKFGRVYKRDEVVFR
                     ENDSGCEMFYVLSGRIRLERSDGHVKKTLAEMGPGQYFGEMAALIDVRRTATARALED
                     SHLAVIDDRTFQSLIGESREAALAMLREFAARLKNSNTTLEEFTHRRTQMLILMQILE
                     HPEDAVDDHIRQISRLTRKEPDQIGEIMRDLSDRGILTGEGDQAPRINHDKMWSMLDS
                     GT"
     gene            19058..20818
                     /locus_tag="PLR47_00885"
     CDS             19058..20818
                     /locus_tag="PLR47_00885"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG11516.1"
                     /translation="MILQPRTDHRLWPFILCLVLLWALTAQAREAPPVRVGNAVPAVE
                     IAPRAEYLLDYSDHLRYRDIAEGRLPFQRHEKYSFQFSFKRATLWFKCRIAPADSGDD
                     EPLASRRSFLVFDNAALGAITLYVPVIKDGRPDFIEMDGGWQQGKKSREFPFLYPTFV
                     LPENLDASRPVVVRVATPYALQFRATLYTIDAFRKTSFVLFLIVGFFAGILVAMILYN
                     LALYLFIRDRQYLYYITYIGFLLLWQCVLVGLFRYIWPPLGEFLMSGIALFAALMMIF
                     AVSFGIVFLNTPKTAPRHDKLLKGLAVFMGSIILMVLLRQLWLSNLLSYLSGQVVTVV
                     LFTAALSSLRSGFRPALFYLIAFGVFLLAAFVFFFKFYGLIPNNTFTMHIVIFGSAAE
                     AILLSFALGYRIRIMQEEEEQLRERERDLEAISVTDELTGLFNRRFLNPALVKKTAAA
                     RRSGTRLSLLMLDVDHFKDFNDTYGHPEGDKVLAALGRLLVQTLREEDIACRYGGEEF
                     VVILHNTDIRAALEVAERIRAGFEKISFQPGLSREIHATISIGAAELLTDENPEGLIS
                     RADQAMYQAKQTGRNRICRA"
     gene            21031..>21958
                     /locus_tag="PLR47_00890"
     CDS             21031..>21958
                     /locus_tag="PLR47_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOG11517.1"
                     /translation="MKAAVLHGVRDIRIQTVPDPVCAPHGVIVKVNACGVCGSDLHWY
                     KENGHEGAIFGHEFSGDIVEVGKNVQGIAAGTRCTAVGFSPCGKCFWCKQGKMHRCSD
                     MALLGYQFPGAMAEYVHVPFAIPGRSVFPLPEELSYEDGASVEPLSISYFSVNRGQPK
                     PSDIVAVIGLGVIGLYAVQILKALGVEKVLAAGRRPSRLAAAKRFGAHPVIDAAAEDT
                     LQAVMQATDQLGVNTVLECAGNQTTFDQAVAMARGGGKILLVGIYEEALHWQPLSVIG
                     KNLTLVGCLGGNFPAVIELLKTKKVSTENIVSH"
BASE COUNT         4819 a         6146 c         5858 g         5125 t
ORIGIN      
        1 cggcatcggg atcggctgcg aaaaagtcgg gcatattttc atcgaccggt ccaatcatga
       61 aaaggccctt gcctcgctgc gggcggcgaa agaaaagatc gtcaacggca cgtcggtgat
      121 ctttttcccg gagggcaccc gaagcaggga cggctcgctg ggcgtcttta aaaaaggggc
      181 cttcaagatg gccgtggatc tccgcctgcc gatcctgccc atcaccatcg tcggcacgcg
      241 cgatattctg cccacggact ccgtcgatct ctttcccggg cgggcgcgca tgatcatcca
      301 caagcccatc gacaccggcg gctacaagga tgacaacctg gacgaactgg tggaccgggc
      361 gcgcggcatt atcggatcgg ggctttggaa agggacgccc gaataacact ctgcatccgt
      421 cggccggcgc ggcccgcggg ccctctcaat tcttcaaatc gtatttcttc agcttgtagc
      481 gcagcattct ccgcgtcatt ccaaggcttg cggcggctct cgtctggttg ttgtcttcct
      541 tggccattgc ctcaataatc agattttttt ccagttcttc ccgcatttcc aaaagagatc
      601 cggagatgtt cctgcctgaa aaattattaa aggattcatt tttgaaaggc agatcgccta
      661 cggttatata gtcttcccgt gcgatgacca ccgcgcgctc cataatattg accagttccc
      721 gaatattgcc cggatagtcg tatttcaaga gcaaatctct ggcctccgcg gtcagcccct
      781 gaatgttttt gccgttttcc cgcgtaaatt tctccagata atgatcgatc agcgccgtca
      841 aatcttcttt tctttcgcga agcggaggca gggacacggc aacgacattc agccggtaaa
      901 acagatcttc ccgaaaagct ccgtcactga cacgggcatc cagatcctga ttggtggcgc
      961 tgatgattcg aacatctacg gaaatgctgg cattgctgcc gagccgtgaa atctcccgct
     1021 cctgcagaaa tcttaataat ttgacctgaa tggcggggct cagttcgccg atttcatcca
     1081 gaaaaagagt gccgccgtcc gcttcttcaa agcggccgat gcgcctggcg tcggcgccgg
     1141 tgtaagcgcc cttttcatgg ccgaacagct cgctctccag cagattttcc tgcagggcgc
     1201 cgcaattcac cacgacaatg ggcctggaag cgcgcgggct caactgatga atcagcctgg
     1261 ccagcagttc cttgccggtg ccgctttccc cctggatcag cacggatgcg cggctggccg
     1321 cgacgcggcc ggccaggttg atcagttcca ccattcggga acttttataa attatttttt
     1381 ccgcggtgac gccggttttt cccagctcct gcttcagaag gtcattttca cggatcagac
     1441 cgcgccgctc cgccacccgg tccacgagaa tcagcagttc atccagttcg atcggcttgg
     1501 tgatgtaatc cacggccccc gccttgatcg cctccacggc ggtttcaatg gtcccgtagg
     1561 cggtaataat cacgacatcg atttcatgat tgattttttt gacttcctcg aggacttcca
     1621 gtccgctcat gcccggcatt ttatagtcca gcagaagcaa atcaaaatga cggttcgaaa
     1681 ccgctgaaac ggccgcttcg ccattttccg cttccgtgac cgcatgtcct tcggaaatga
     1741 gaaaatcccg aagcatttcg cgctgcgatt gtccgtcttc cacaatcaga atattcaatt
     1801 tgcgcatttt ttccgtcccc ggcgtcaggt tttttcacac ttttttttga tcggcagcac
     1861 aatttcaaac gtcgtcccct cacctaggcg gctgacaacg cgaacatcgc cgccgtggcc
     1921 gcggacaatc tccgtggcca gtggaatgcc caaacccagt cctttttcct tggtggtgta
     1981 ctccggactg aatatttttt cgatttcttc cgacgtcatc ccgcagccgg tgtccgtgat
     2041 gctgaccaag atattatttt tgccttccct gacgacggta atcgtgatga cgcctttctt
     2101 tgaaatcgac tccatggcgt tcttgatgaa attcaggaat gcctgctgca gtttgttgat
     2161 atccatggga atgacagccg gactcaactg ccatttcgtt tccagcgcaa tcccccgtga
     2221 gccggcctcc tccgcaagca aactgacaat tttttgcaga acctccgtta cggaaaaatc
     2281 cctcagctcg aggcggcggc ttttggaaaa ggaaagaaat tcttcgataa tggcgttgag
     2341 ccgccggatt tcatcgcgga tgacggtgga aagattattg aattcatgcg ccttgagctt
     2401 atcggccggg acataatccc ttttcaggcg ctgcgtggcc atgctgatgg cgttgagcgg
     2461 attgcggatt tcgtgggcca cccccgcggc gagctgcccc agggaagaaa gccgctccgc
     2521 cttttccagc cttcgttcca tttcgataat cccggccaga tgccggttct ggtcgtggta
     2581 gagaagccac atggacaaaa gagcgattcc ggcgacaaac aacatgaaaa taaaaatatt
     2641 ctggcggttt tcggcaatga ttttatccat ggaaccgcgg tccagaccga tgcggacgat
     2701 cccggcaatc ttttctttcg gcagataggg gttttccaga atgaagggag cggctacatc
     2761 cagaatcttg attccctgcg ggtccacttt cctgctcaag attccttttt tccctttcat
     2821 catttcctga tcgttgagat cccggctggt caaaccggcc ggcaattctc cgatatttcc
     2881 cagcaggatt ttttgatcat taaagacctg caggtaggcg atgccgtggc cttcgcccat
     2941 tttgctcatg gccctttcaa cggcgacttt cagactccag tacaacctgc cttttcggtc
     3001 cagacagaag acggcgattc caccgcctct ttttagtttt actccgacgt atccgatgtc
     3061 ctccttgatg cggatttttt ccagcagttc aacggtcgtt gccttgcgcc cgttgatgaa
     3121 caaatcgctt ttcgacagca tgtcggtttt aaggggaccg ctttgataga ggatatcgcc
     3181 tcgcatattc agaatggcca cgtaccaaaa ataatgatct tccgcaaact tttgaaggta
     3241 ggcgttgctc attaatttct ttttccactg ttcgtcgatc tgttgcccca gtttggtgat
     3301 ggcatcgtaa accagctgga tggaataggc ggactccgcc tctttggccg ttttgagatg
     3361 ggacggcatt ttccctttgg cggcggcaat gcttttcaga ttatcttccg tctggcgcaa
     3421 aaggacgctg atggtggtca gggcctgatc ttccataaat ccgaccaggt tggtttcgtt
     3481 gcggcggata tccagatagc ccatcaccag aatcagagcg accaggactg ccgacacgat
     3541 gccgacggcc agcggctgga gaaattttcc cttgtaggga cgcttgccac gttggacaat
     3601 agtttctttt ttttgaaaaa ttctcatgtt gtccttattt tgtgcgcaca tgaaaaacgc
     3661 ttttgaaatc taatttgtac gttttccagt gatcattgtg cttttcaaga aaaaagtcaa
     3721 catggaatac tgctcaggtg gacatttttg tccaaatgtg gacaaaaatt gtctttcgtt
     3781 catctatcta atattacttg ataaattttt ttatcgatcg tcaattggac atttgttgtc
     3841 aaaggttttg tcttgaaatg gaagcaaaga aggtggttta tttattaatc tttttacaaa
     3901 tcaataactt acaatataaa tccctctgtg gcatggtatc tgctagataa ccaatcagaa
     3961 aaagatataa gtgtcgacta aaatatcacg ggcatgatta gccgacagca aaaataaccc
     4021 gggaaggtaa ccaggccttc actcttctct cctgacggga ggcttccaac aagcctcccg
     4081 tttttttatc cttttttctc cgattccttt tttgctttgt ttttccggcg ccgccactct
     4141 tcgactcttt ccctgatgat tttttcatac ccggcgtcgg tcggggagta aagcactttt
     4201 ccctgaagct tttccggaaa atactcctgg ggaacgaagg catcctgata gtcgtgcgcg
     4261 tatttgtagt ccttgccgta ctccagttcc ttcatgagtt tcgtcggtgc gttgcggata
     4321 tgcaggggaa cgggaagata gccggtttta tgaattaaat cctttgtttt tccgtagccg
     4381 gtgtacaggg aattactttt gggcgccacg gccagataga cggccgcctg cgcgatggcc
     4441 agttctcctt ccggcaggcc gatgaaatga aacgcctgct gggcggccat cgtgagcacc
     4501 agggcatggg gatcggcgtt gccgacatct tcggaggcaa agcgcaccat gcgccgcaga
     4561 atgtaaagcg gatcctctcc ggcggccagc atgcggccca gccagtagag cgttgcgtcc
     4621 ggatcgcttc cgcgcatgct tttgtgaagc gcggagatca gattgtaatg ttcctctccg
     4681 tccttgtcgt acaggagaga cttcttcagc agcgcttctt cggcggattt cgccgagatc
     4741 atcctttctt cctcaggcag cgactggacc atcgaggccg ccgcctccag gttgttgagc
     4801 gccgtccggg catcgccgtc ggaaatcccg acgataaact taatcgcttc gtcgtcgatt
     4861 tgaatggcaa attggcccag acccctttgc ggatcggaaa gcgcgcgctt cagaatgccc
     4921 gtcaggtctt cttccgcaaa ctgtttgagc agcatcacgc gggttctgga cagaagcggc
     4981 gcgatgactt cgaaagaagg gttctccgtg gtcgcgccga tcagcgtgac gagcccgctt
     5041 tccacgtgcg gcaaaaaagc gtcctgctgg gccttgttga agcggtggat ttcatcgaca
     5101 aacaaaatgg ttttctgctt tttggcctcc cagnnnnnnn nnncttgacg ccggaaagga
     5161 cggcggaaaa actgacaaag ttggctttgg tttcattggc cagaatccgc gcgagcgtgg
     5221 ttttgccgga acccggcggc ccccagaaaa tcatcgagaa aagcttgtcc tccgcgatgg
     5281 atcgccgaag caaggttcct tcgccgacca ggtgcggctg gccgatgtaa tcggcgagcg
     5341 taagcggcct cattctttcg gccagcggcg ccgtctccag cgcgtccgcc ggagttccct
     5401 ggctgaataa atccaggttc ttcatgtttg cctctttgtt ttttcgagaa tttcaatcaa
     5461 gctgacaacc ttgtctttgg gcaggatttc cgcgaagcgc tttagctcct cctgcgtcag
     5521 gttttcctgg agcagcgaag ccgcgtcaaa cgcggcctgc cagcagaaaa gaatccgcgc
     5581 gcagggcaga ttccccgctt cccgcaggca gtaggcaaac gtcatttcgt cgcccagttt
     5641 ggggcacctg gtgattttat gatcatgcgg tgttttcatt gcctgccacc ttggcgaaaa
     5701 aaagaaaagc cgtcaagaaa ttttatttcc ctgacggcct tcgcagggaa cggccgcgac
     5761 cgttcacgca aaatgaagtc cttatggttt cgggaaatct ttaatggcca gcaaggctcg
     5821 ctggatttcc tcatccggca gctcataggc ctccagtttt ccggaaagat aggcgtcata
     5881 agaggccata tccaccaggc cgtggccgct ccaattaaag aggatcactt tttccttgcc
     5941 ttcttctttg gcgcgcattg cttcatcgac aacggcggcc aatacgtggt tggtttccgg
     6001 cgcgggaatg aagccctccg agcgcgccca cttgacgccg gcgtcaaatg tttttaactg
     6061 gtcataggcg cggggttcga taaatttttc gcgaaccaga tggctgacga tcggggccat
     6121 gccgtggtag cgcaaaccgc ctgcgtgaat cggtgcgggg acataatcgt gccccagcgt
     6181 atacatgggt aaaagcggcg ttttctgcgc cagatcgccg aagtcatacg cgaacgggcc
     6241 tttggtcatg ctgggacagg aggacggctc cgctccgaca attttcacct gcttgccatg
     6301 gattttatcg cagacatacg gcgctgcgat accggcaaaa ttactgccgc cgccgcaaca
     6361 acccatgacg acatcgggat agatgccgag cttctgcatt tgcttttgag cttccagacc
     6421 gataatcgac tgataaatca acacatgatt aagtacgctg cccagggcat agtgggactt
     6481 tactttgctg gtgactgcat cctcgatagc ttcgctgatg gcaacaccca gcgagccggg
     6541 ggaatcgggg tacttggcca ggaaatctct gccggattgc gtcaaagtgc tggggctggg
     6601 aatgcattcg gccccccagg tgttcatcat caggcgccga tagggttttt gtccatagct
     6661 gatttttacc atatagacac ggcactccag gccaaacatc tggcaggcca tggccaacgc
     6721 gctgccccat tggccggcgc cggtttccgt ggtgattcgc ttggttccgg ccaccttgtt
     6781 gtaataggcc atcggaagag cggaattggg tttatgcgaa ccggcggggc tgacaccttc
     6841 atttttataa taaatcttta ccggacagcc cagcagtttt tcgaaattgc gcgcacggca
     6901 gagaggagat ggacgccaca gcaggtactt ttgcagcacc tcatcgggaa tatcaatcca
     6961 gcgctgcgtg gagacttcat gttcaataat attcatgggg aaaatggcgg ccagatcgtc
     7021 gggtgtgacc ggctggccgg tgccgggatg cagcggcggt ttcatgggtg tgggcaaatc
     7081 cgccatgatg ttgtaccatt gtcttggtat ttcttgatct tccaataatg ttttgacctg
     7141 ttccatattt tttctctcct ttgccagaat gacccttgct tttttcagca gactcttttt
     7201 gcgcccggcc gcaccggggc caaaaaaaat gccatggggc ttttcttccc atggcattct
     7261 tcatatatcg acgagtcacg ggaaggagcc tccctgccca tgctcggtga ttttcaggag
     7321 cttacgggca gacattcatc gcctgccacc accagtttct gtttaaaatg tttgtcatca
     7381 catctaccat cctctttcgt cctgcggttt tccactaaca taaacaaaaa atgaatgtca
     7441 agccgaattt tgctcgccag tcgtttgaca acaaaaatca gctatgctag aggacatagc
     7501 cgtgaccggc gtatttttaa gcggattcac gaaacttcga ataaccaact tgaaaaataa
     7561 ggcagacaga gcacctacaa aacgttaagg agagtgaaac catgacccag agtgaattca
     7621 aaaagatcat tgccaacgcc attaaagccg aagtagcagc caataaattt tacagcggcg
     7681 tagccaaaaa agcgaaggac gcaaatctca aaaagctttt caaagaactg gccgacgaag
     7741 aagcacagca caggctcacg ttaaaaggtt atgcggccag gggggctgaa aaatttactt
     7801 ttaccgaatc ggcggattac aaggtggcgg acgagcttcc cacgcctccg ttgacggccg
     7861 atctgaaacc ggttgacggt ttggtcatcg ccatcaagaa cgaacttgcc gccatgcaga
     7921 tgtacacgca attggccaaa gcaagcgcgg acaaggcgca gaaaaaggtt ttccaggagc
     7981 ttgcgtcgat ggaacgcggc cacaagaaaa aactcgagga tttgtacacc aacatggctt
     8041 tcccggaagt ctggtaatac gaccgttgca ttcattcgga ttcccgacag aacccggtaa
     8101 aacaaaaaag tcagggcgtt gcggccctga cttttttttg cggatcacct gtgcgtttta
     8161 ccggtaatat cccgtcccga ccacccagcc gaagggttta aagagcagaa cataggaccg
     8221 cttgggcaga gacgcggcct ggccctgctt ggtaaaccgg tattcgacat agccgccggt
     8281 tttgcctttg gccagaaatt ccttcacgta gaaaacgcct ttggcgtccc ggtcgttcag
     8341 gcggtttctc ccttccgtat ccttgcggcc gtaaagcacc acgttgacgc cctccgtcgt
     8401 gtcggcccag aaatatccat cctttccata gcgcagttcc cgcagcaggc cggcggccag
     8461 ttcctttgct ttatccgcgc tcatctcccc tttctgctgc cgggcgtgaa tcgccgccag
     8521 caggctgacc gccgtttcca cctggctctt gatcagcgag tcttgagcgc cgtccgaaag
     8581 cggcggcgtc tgcgccaaaa ccgacatgca aaaaaccaca ctcagcaacc ctcccaaaac
     8641 cgtcaaccag accttgcgca tatttcctcc ctgatgagat gattttcgtt tcaaagccta
     8701 aaacgccttc cttgcgcctg attttgccaa ccggctgttt tttataccag cggcccggaa
     8761 aaagcaaata atcctgattg aataccggac caaattgtgt ttagaagcat caacgccata
     8821 aaggaacgtg tcatgaacca gccatccagt ccggtcgaga ttgatttcaa ttccgaattc
     8881 cagcgggcga tttcgctcat ggaggatacg cagaagcata tcctgatcac cggccgggcg
     8941 ggcacgggaa agtcaaccct gcttaagtat ttccgcgaca ccacgtcaaa aaaggcggtg
     9001 attctggcgc cgaccggcgt ggcggcggtc aatgtcgaag gccagacgat ccattccttt
     9061 tttcatttca agccgaatgt gacgccggcg tccatcaagc gcaggaaaaa ggcggaaaag
     9121 gacaggctta cgctctatca aaagctgacc accatcgtca ttgacgaaat ttcgatggtg
     9181 cgcgccgatc tgctggactg cgtcgataaa tttctgcgct tcaacggccc cagcgaaaaa
     9241 aagccttttg gcggcgtgca gatgattttt atcggcgatc tgtatcagtt gccgccggtc
     9301 gtctcatcgg cggagcgcga gattttcaaa aaccattacc cgacgcccta ttttttcagc
     9361 gcaaaagtct ttgaggcgct ggacatcgag tatgtcgagc tggaaaaagt ttaccggcag
     9421 aaggacgacg agtttgtccg tctgctcaac accatccgca accgctccgt gacggatgac
     9481 gatctggcca aattcaattc tcgctgcgac ccgtttttcg aagcgccttc aggatccttt
     9541 tacctggccc tcaccagcac caacgacctg gccgacacga tcaatgaaaa gcggctggcc
     9601 gaactgccgg gaaaaatctg gaaagccaaa ggacaaatcg aaggcgattt cggaaaggaa
     9661 tatctgccga ccgcggtgga tttgaaactg aaaaaaaacg cgcagatcat gatgctcaac
     9721 aacgactctt tcggccagtg gatcaacggc accatcggaa aaatccgcaa attcgaaaaa
     9781 gacgacgaag gagacgacgt gatcgtggct gaactggaca acggcgatac ggcgcgcatc
     9841 tcgccctata cctggaaaat ctaccggttt ttcatcaaag atgaagagct tcgctcggag
     9901 gaggtcggat cgttccggca gtacccggtg cgcctggcct ttgccgtcac cattcacaaa
     9961 agtcagggaa aaacctttga aaacgtggtg atcgacgtgg ggcggggcac ttttgcccac
    10021 gggcagatgt atgtggcgct gtcgcgctgc acgaccctcg acggcattgt cctcaagaag
    10081 cctcttcaaa aaagccacat tctgatggac tggcgcgtgg tgaaatttct caccaatctc
    10141 cagtacgccc gggccgccaa aaccctcagc cgcgaggata aaataaaatg gatcgaagag
    10201 gcgattgctg aaaagaaaga catcgaaatc ctttacctca agagtcagga cgaaaaatcg
    10261 cgccggacgg tgaggcccct gttcgtgggc aaaatggagt acaagggcta tccctacctg
    10321 ggcctcgaag cgatctgcct ggagcggcgg gaaaaaagaa ttttcaacgt ggataaaatt
    10381 ctggaaatcg gagcggaaaa gcggtagcat ccggcacttt tttttcaaga cggccgacgt
    10441 cccggggaac gcaccatggc aaaacagatt ttcatcgtcg atgacaaccc caacaacctt
    10501 tatctgctgg ccagcatttt gaagagccgg ggatggagcg tccgtgaggc gggaaacggc
    10561 aaagacgccc tggacgccat ccgggttcag ccgccggacc tgatcgtctc ggatattctg
    10621 atgcccgtca tggacggcta cgccctgtgc cgggaatgca aggcggaccc gaagctgaaa
    10681 tccatccctt tcgtctttta taccgccacc tataccgaac cgaaggacga ggactttgcc
    10741 ctgagcctcg gcgcggaccg atttctgctc aagccccagg agccggaaat cctgaccggc
    10801 atcctggagg agttcctgca cgagggaaaa gccgccccgc ccgaagcatc ctcacccctt
    10861 ggggaagaca tggagttttt ccgtcggcac aatgaggctc ttttccgcaa gctcgaaaag
    10921 aaaatgcagg acctggaagc cgccaaccgg gagctccgca tgctggaaga aatctaccgg
    10981 cagagttttg agcatgtcac cgatgtcatt tttaccgtgg acgccggcct caggatcatc
    11041 agcatttctc cgagcgtgga gaaagcgctg ggatacaaac cgcgggaatt catcggccgg
    11101 tccattcggg accttggata tctcttcgcg gcggattcga tggaaacggc cctggaacac
    11161 atcggcctgg ttctgcaggg gacagccctt ccggcgacca tttacgaatt cagggccaga
    11221 gacggggcgg caaagttcgg cgaagtcagc ggttctccca ttacccgcga tggaaaagtg
    11281 gccggtctgg ttgcggttgc ccgggacatc accgagcgca gactggcgga gcagaagctg
    11341 cgggaaaatg aaaagcgata caaactcctg acggaaaaaa tgacggatat cgtctggatt
    11401 tcggacatga atctgaagac gctctacgtg acgccctccg tccagagcgt gttgggattc
    11461 agccaggaag aacggatgca ccaggcggtc gaacagcaaa tcacgccgga gtctctctcc
    11521 cgcggactgg aagccatggc ggcggagctc gccctggaaa aacaggccct gggggatccg
    11581 acccggctgg cgacgctgct cctggaatat tatcacaagg acggctccac ccgctggatg
    11641 gagaccatca tcagcgggct tcgcgacgat cagggagtcc tgaccggact tcacggcgtt
    11701 tcgagggatg tcaccaaacg caaaaaagcg gaagaggaaa agcaaaagct ggaggagcgg
    11761 ctgcaccggg cggaaaagat ggaggctctg ggaaagctgg ccggaggcgt ggcgcatgat
    11821 ctgaacaatg ttttgggggt tctgtccggc tattcggaac tgcttctgct ggagcttccc
    11881 gaagatcaca aagccagaaa aaacgtggag aagatcctcc agtccacgga aaagggagcg
    11941 gtgatcatac aggatctgct cacgctggcc cggcggggag ttgccgtgtc gggcatcgtg
    12001 aacctcaatc ggatcgttga cggttttctt aaaaccccct tttttgaaaa aataaaaaac
    12061 gaccatccgc ggataacctt ccgggcggag tgcgaccccc gtcttctgaa catcaaaggc
    12121 tcagccgttc atctggaaaa agccctgatc aacctggtgt ccaacgcggc ggaggccatt
    12181 gccgaggcgg gcgagattgt tatccgcacg gaaaaccgtt acgtggacag aatcctgatc
    12241 agccatgaag aagttcagga gggggattac gtcctgctga tcgtttcaga tacgggcatg
    12301 ggaattccgg cagagcatcg ggagaagatc tttgagccgt ttttcaccaa aaagacgatg
    12361 ggatggagcg gaacggggct cggcctgccg attgtctggg gaaccgtaaa ggaccagaac
    12421 gggtatattg accttcaaac gaaggagggg aaaggaacaa ccttcatcct ctacttcccc
    12481 gtaaccagag aggaggcggc gctgccgcag caaaggaagc cgctggagga ctaccggggc
    12541 ggcggggaac ggattctggt tgtggatgac attgccgagc agcgggaaat cgcttccagt
    12601 ctgctgatga aactgggcta tgaggtcgag acggcttccg gcggagaaga ggcggtaaaa
    12661 ttccttcagg agaaggaggt ggatctggtt ctgctggata tgatcatgtc tccggggccg
    12721 gacggtctgg aaacctaccg gaaaatccgc gacatcgacc cgcggcagaa aacgattctg
    12781 gtcagcggat tttccgaaac cgaacgggtg ctgacggcgc agcggctggg cgcgggcgcc
    12841 tacgtaaaaa aaccctacgt catggaaacc atcggcctcg cgatccggca tgagctgaag
    12901 cggtaagacg gcaaaaggcg gtcttcaccc tttgctgcgc agctatttca taaaaattga
    12961 tccgacaatc ggcgcgaaat aaaaggcgga caggatcaac gccacggcgc ccgccgcgga
    13021 aatcagccgg tcgatttccg ccatggtcgg gccgcggcct gttttcggtg ttttgcgcat
    13081 tgccgccgca aacgatttcc gactcaacgc gacgcttcga tggccgttcc cgccgcaggg
    13141 ccatccggaa acactgatga tcgcacgcat cctgattgct ccattttttt gatccgggtt
    13201 acatcagata ttttctgacg gaggcggtga tcacgccgcc tattttgagt tcttctttgg
    13261 gacaaatcgc agcataagcc gggttggcgg cctccagaac aacttttccg ccccgccggc
    13321 ggaaatattt catggtccac tggccgtcga cttcggccag aacgatgtcg ccgttccggg
    13381 gcgttcgctc ccgctcgacc acgaccaggt ctccctccat aatgcccgcg ccgatcatcg
    13441 agtctcccgt gactctgagc agaaaagaag attcgggtct ggtcacgaga tactcgtcca
    13501 tggacagaac atcccgaggg atttcctccg ccgacgcggg aatcccggcg gccacattgc
    13561 cgaccagggg aatgccgaag gaaacgcccg acagcttgag aaatccctgc gcgtctttat
    13621 ccagaatgcc tttttcgatc agcttctcga tccagtaaga aacgacgctc ttggaccgca
    13681 cctcaaagag atccatcatc tcggcgtacg tgggcatgcg cttctgccgg cggtaaaacg
    13741 acaccaggct ctgttcgacg gattttatgt cgcggacttt tttcatggcc cgtcttatat
    13801 agaacgcacg ttctattgtc aagtgttttt ttaaccgtag aaaacggttt gcatcaccct
    13861 tgcgggcggc aagaccgttc tctgctaaag gctcgaatat gccagcttat ctcgtcatcg
    13921 tgtatttctg ttcgtctttg ggaacgtacc accggatgaa gttgtggccg atgccggcag
    13981 gcgccggctc tatgccgcga acgcggttcg aaatgatgat taaatcgtcg ggaacataca
    14041 aaaaagtgta aggctgatcc tcggccagga tttcctgaaa acggtcgtag tatttcttcc
    14101 gttcggcctg gttgaaggtg cttctgcctt tctccagcat ctcgtcggct tcgggattcc
    14161 cataggaaac aaagttgagc tcttccggcg cggttttgcc ggagtgccac acgtcatagg
    14221 cgtccgggtc cggcggaatg gtccagccaa gaatcaccgc gtcaaagcgc cgcttgttga
    14281 tgaaatgagt gacaaacgcc gaccattcca gaacgcggat tttcgcagca atgccgactt
    14341 ctttgagctg ccgctgaatg atctccgcgc atttctggcg ggtctcattg ccctgattgg
    14401 tcagaatttc aaattcaaag gaccggccgt ctctgtcgag taccccgtcg ctgttcgcgt
    14461 ccgcccatcc ggcctgcttc aggagttcgc gcgctttggc cgggtcatag tggtagatct
    14521 tcactttgtc gttgcatgcc caggtgcccg gcttgtaagg ccccgtcgcc ggcttgccca
    14581 gtcccagcag cactccgctg atgatttcgt ctttattgat ggcgtgggat atggcctgcc
    14641 gcacgcgccg gtccgcaaaa agcgggtttt tcaaattgta gcccaggtac gtgtaggcaa
    14701 acgccagata gcggtatttg ttgaaattct gccgaaacag gttgttttcc gtctgccgcg
    14761 tgtactgcag cggcgtgagc cccatcatgc cgatattctg cgcgcgaagc tccagaaaca
    14821 tggtggcggt atcaggaatg atccgcgtaa tccggccgtc cagacagggg cgcccctcga
    14881 aataatcctc gttggacgcc agaacgattt tctgacccgg cacccattcc ttgaagcggt
    14941 agggaccggt cccgacgggg tggcgcgaaa gagggctttg cgcaatgtct tttcccgcca
    15001 gcaggtgccg gggcatcacc gcagaagacc aactgatcaa agcgggcgcg aaaggcttgt
    15061 cgtaggtgac gcgaaaggtg tggtcgtcca gaaccagagc ctttttgact ttctgaaaat
    15121 ccccggcata cgcggtgggt gttttgggat ccaccgtcac ctgataggtg tacagaacgt
    15181 cggcggcggt aaagggccgg ccgtcgtgcc accggacgcc ttttcgcagg tggaaggtga
    15241 tcgacagacc atccggcgaa atctcccagg attcggccag atcaccgacg atattcatat
    15301 ttttatcata cttgaccaaa ccgttgaaaa ccatgccgcc cacggcgtgc gacgccgagt
    15361 cggaagccag aagcggaatc aggttgctgg catccccgat atcgccccgt accaggatat
    15421 cgccgtaggc gggcggcttg tcccctttgg aaccggcgct tcccgtctgc ggcgtccggt
    15481 cgcacgcggc ggctgccagc agcaaaagga ccagtacgaa atgaaaaatc ctggcggcag
    15541 aaggcctggc atctttgatt ctgaggccga agcggtccag ggaatgaagt tcaacgcccc
    15601 tgtcaaaacg ggagaaagag gcgttcgttt tcatattcga tatcctgcgc atggcgcaat
    15661 acatagccca ggaagcaagt ctttgcaata tcatttactc tttcgcgcct cccccggccg
    15721 ccggttcatc cgccgcaatt aatcctcgct ttttcagaaa atctgtgaac agacttttcg
    15781 tgaagacaaa gaaaccatcg agggcagagg atcatgaaag tcaaaacgca ggagctgttt
    15841 aatttttatt cacactttgc cggagcggtg gccgccgtcg tcgggaccgt ctatctggcc
    15901 atggccgcca gtgattcggc ctccggcctg gtcaccgcgc tgatttacgg aatatccgtt
    15961 gtttttcttt tttccgcgag cacgctctac catgccttca aaaaagagga aaatgaactg
    16021 tccttctgga gaaaaatgga ccggctggcc atttttttca tgattgccgg aacctttaca
    16081 cccatctgtt atttttgtct ggaggatccc tacagatggt ggatgatagc cttccagtgg
    16141 gggctggtgg gtgtcggcgt catttcgcag attttttttc cgggggcgcc cagaaaaatc
    16201 tacgcgatta tttatctgtc catgggctgg tccgcgcttt tcacgatcaa gcaaatgctg
    16261 tccaatatgt cctcttccca ggccgtcctc ctcgtttcag gcggcattgc ctttacactg
    16321 ggcgccgtta tctatgccgt caaaaagcca agaatgattc cgggtgtttt cagttttcat
    16381 gaactttttc acatcatggt cctgatcggc ggcgttttgc attacgccat gatctacggc
    16441 gtttattccc gtcccgttta accatgagca cggttccttc tcccgcgcaa cccggacgtc
    16501 aaacggatct gctcctggcg gaggaactga aattatttct tcaggattac gaaaaaaatt
    16561 ataaggcggg cgagagcaaa cctccggata tcggaatgcc tttttttctg cgcgcgcccg
    16621 ggtccgacac cggcgtactg ctcattcacg gactgatggc ggcgccggcg gaagtccgcg
    16681 agtgggcgga atttttgcac gccaggggta tgacggttta cgcgccccgg cttgccggcc
    16741 acggaacatc cgcaaaagac ctcttggttc gcgattattc ggactggctg aattcggttg
    16801 atcgcggcca cgccattctc aaaacctgct gccgtagaat cctggtggcc ggtttctcca
    16861 ccggcgccgg cctcgcgctg caatccgcca tcgggaaacc ccatgatttt gaagcggtca
    16921 ttgccgtcag cgcgccgctc cggttcaaga gtttttcatc aaggtttgcc gagcttctgc
    16981 atggctttaa tctttgctgc tctcatgtcg gcctgaaccg ccttgcggtg gaattcgtca
    17041 aaaacgacgc ggacaatccc cacatcaatt atctcctctg tccggtcagc tcttttgttc
    17101 aggtcaaaaa gctgatgcgg agcgtccggc ggaggctgcc tgacattcaa attccggcgc
    17161 tgatcctcca gggccggcac gaccccaagg ttgctccgga aagcgggccc gccatctttc
    17221 agcgcctggg cgcgcatcag aagcgtttcg cctggattga tttcaaccgg cacggcattg
    17281 tcctcggccc cgccgcacgg gaagttttcc atgaagtgga gtcgttcctg aacgactgcc
    17341 gcctgacggg ttcctcgaag taatccgcgt tttgcttccg tggcggtcgt ttgacgcaag
    17401 cttcttttaa ataatcattt tgaccggatg cttattccgt tgtaagatgc gcctcaaaaa
    17461 cacgatagtg tcagtcgaat ccatttatga cggaaaaaaa agaagactcc cgggcgcggc
    17521 gcaaaacgcg caaagaatcg atgcagatga aggccgccgg cgcgtgggat atcgccttcg
    17581 acgcggtcaa caacttcagc ctgaacggcg acgccaatca ggcggcggcg atcgcgctct
    17641 atgccatcct gtccgccatt ccgcttttta ttctaaccct catcgccgcc ggatccttct
    17701 tcagttccaa caccactctt cagactgaca tcatcggcgc cgttcagggg gttcatccct
    17761 atttttccga agcgctcctt cagcaggtgg ggcaaatcga gggcaagagc aaggtgctgg
    17821 gctggatcgg acttttgggc ctcgtctggc tgtcggccgc gatcttcaac gccatcgaat
    17881 cggcgctgaa catcattttc cggtcccggc gcaaacgaaa ttattttgtc tccaaactga
    17941 tggccgtcgc catgattccg gccgcctggc tgatcggcgg tttaagcgtc gtcattacct
    18001 atctggcggc gctgctcgca aaggagccct ccggctgggc cggcggtgtt ctgttttccc
    18061 tgacaatgcc ggcgcaaatc gctctgcgtt atctcattcc ctttctgatg gtcgttctcc
    18121 tggtgacctt cgtgtaccgg atcattccca cggcaaaaat ccgtttttcc gtggttctgg
    18181 ccggcagcgc gatttttgcg tttctggtgg aaatcgccaa gcagcttttt acctggtacc
    18241 tggccaacta cacccgctat aatgtcatct tcggctccct cgaaaccgtg gtcatcctgg
    18301 tcatctgggt tttttacgtg gcgctgattt ttctgttttg cgcggaaatc atgtcttcct
    18361 tcgagcggcg cgacatgctg cttttggaaa aagccctgct taaacctcat gattctctcc
    18421 tgaaggtgga tgcgcgcctt ttccagaaat tcggccgcgt ttacaaacgg gacgaagtgg
    18481 ttttccggga aaatgattcc ggctgcgaga tgttttacgt gctttcaggg cgcatccgcc
    18541 tggagcgttc cgacggccat gttaaaaaaa cgctggcgga aatggggccg gggcagtact
    18601 tcggtgaaat ggcggcgctc atcgacgtcc gaagaaccgc gacggcgcgg gcgctggaag
    18661 acagccacct ggccgtgatc gacgaccgga ccttccagag cctgattggc gaaagccggg
    18721 aggcggcgct ggccatgctg cgcgaatttg ccgcgcgcct gaaaaattcc aacacaacgc
    18781 tggaagaatt tacgcatcga agaacgcaga tgctcatcct catgcaaatc ctcgagcacc
    18841 cggaagacgc cgtcgacgat catattcgtc agatttcccg gctgacccga aaagagccgg
    18901 atcaaatcgg agaaatcatg cgggacctat ccgaccgggg cattctgacg ggcgaaggcg
    18961 atcaggcgcc caggatcaac cacgacaaaa tgtggtccat gctggactcc ggaacctgaa
    19021 aaatctcgtt tccgggcgca tcaaaggagg aatgaaaatg atcctgcagc cgcggacaga
    19081 ccatcgactc tggcctttca tcctttgcct tgtcctcctg tgggcgctca ccgcgcaggc
    19141 ccgggaagcg ccgcccgtcc gagtcggcaa cgccgtcccc gccgtcgaaa tcgccccgcg
    19201 ggccgaatat ctgctcgatt actccgacca cctgcggtat cgcgacatcg cagagggcag
    19261 gctgcccttt cagcgccacg agaaatattc ttttcagttc agctttaaaa gagcgacgct
    19321 ttggttcaaa tgccgcatcg ctcccgccga ttccggggat gacgaaccgc tggccagccg
    19381 ccgctccttt ctggttttcg acaatgccgc cctgggcgcc atcacgctct atgttccggt
    19441 catcaaagac ggccggcccg atttcatcga aatggacggc ggatggcagc aggggaaaaa
    19501 gagccgggaa tttcctttcc tttatccgac cttcgtcctg cccgaaaacc tggatgcctc
    19561 ccgtccggtc gttgttcgag tcgccacccc ctacgcgctg cagtttcgcg caacgcttta
    19621 taccatcgac gctttccgga aaaccagttt tgtcctcttt ctgatcgtcg gatttttcgc
    19681 cggcatcctc gtcgccatga tcctctacaa tctggccctg tatctgttca tccgcgaccg
    19741 tcagtacctc tactacatca cctatatcgg tttcttgctg ctttggcagt gcgtgctcgt
    19801 cggacttttc cggtatatct ggccgcccct gggcgaattt ctgatgtccg gcatcgcgct
    19861 gttcgcggcc ctgatgatga tttttgccgt ttctttcggc attgtctttc tcaacacacc
    19921 gaaaacggct ccccgccacg acaaactgct caagggcctg gcggttttca tggggtcgat
    19981 cattttgatg gttttgttga gacaattatg gctcagcaat cttctgtcct acctttcggg
    20041 acaggtggtc accgttgtgc ttttcacggc cgccctttcg tctttgcgct ccggctttag
    20101 gccggccctg ttttacctca tcgccttcgg cgtctttctg ctggccgctt ttgtcttctt
    20161 tttcaaattt tacggcctga tccccaacaa caccttcacc atgcacatcg tcattttcgg
    20221 cagcgccgcg gaagccatcc tcctgtcgtt tgcgctgggc taccgcatcc gcatcatgca
    20281 ggaggaagaa gaacagctgc gtgagcggga acgagacctc gaggcgatca gcgtgacgga
    20341 tgaactgacg gggcttttca accggcgttt tttgaatccg gccttggtga aaaaaacggc
    20401 ggcggcgcgg cgcagcggca cgcgactgtc cctgttgatg ctggatgtcg atcatttcaa
    20461 ggatttcaac gatacctacg ggcacccgga aggcgacaag gtcctggcgg ccctgggccg
    20521 gctgctggtt cagaccctgc gggaagaaga tatcgcctgc cgttacggcg gagaggagtt
    20581 cgtggtcatt ctccacaaca ccgacattcg ggccgcgttg gaagtcgccg agcgcattcg
    20641 ggccggtttt gaaaaaatat cctttcagcc cggactgagc agggagattc acgccaccat
    20701 cagcatcggc gcagctgaat tgctgaccga cgaaaatccc gaagggctta tctcccgcgc
    20761 cgatcaggcc atgtaccagg ccaagcagac cggccgcaac cggatttgcc gcgcctgaaa
    20821 caaagcgcgg taaagaagct cgcagggaac ggtcgcgacc gttccctgct gactcgtcac
    20881 tcatcgatca ttgcttgctt ctttgccatc caccattcac cattcaccat tcaccatcaa
    20941 ctatttatat ggtccctcat tttctaacgg cttgacacgg aagccattgt ttgaaatata
    21001 ctttcaaaac tcaaatctcc ggagggcaac atgaaagcag cagtcctgca cggcgtgagg
    21061 gatattcgaa tccaaaccgt tcccgatccc gtctgcgctc cccacggcgt cattgtcaaa
    21121 gtcaacgcct gcggcgtatg cggttccgac ctgcactggt acaaagagaa tggccatgaa
    21181 ggcgccatct tcggccatga attcagcggc gatattgtag aagtcggcaa gaacgttcag
    21241 ggaatcgccg ccggcacgcg ctgcacggca gtcggctttt ctccttgcgg caaatgcttc
    21301 tggtgcaaac agggaaagat gcaccgctgc tcggacatgg cgcttctggg ataccagttt
    21361 cccggcgcga tggccgaata tgttcacgtg ccgttcgcga tcccgggccg cagcgttttc
    21421 ccgctacccg aagagctctc gtatgaagac ggggcatcgg tcgagcctct ttccatttcc
    21481 tacttttccg tcaaccgcgg ccagccaaaa ccatcggaca tcgtggcggt catcggcctg
    21541 ggcgtcatcg gactctacgc cgttcagatc ctcaaggctc tgggcgtaga aaaggttctg
    21601 gccgccggac gaaggccgtc gcgtctggcc gcggcaaagc gcttcggcgc acatccggtc
    21661 atcgacgcgg ctgccgaaga cacgctgcag gccgtcatgc aggcgacgga tcaactgggc
    21721 gtcaataccg tcctcgaatg cgctggaaat cagaccactt tcgatcaggc cgtcgccatg
    21781 gcgcgcggcg gcggcaaaat tctgctggtc ggcatttacg aagaggcgct ccactggcag
    21841 cccctgtccg tcatcggaaa aaacctgacg ctggtgggct gcctgggcgg caatttcccg
    21901 gcggtcatcg aactgctgaa aaccaaaaaa gtcagcaccg agaacattgt ctcgcacc
//
LOCUS       DAOSIM010000009        21894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_2640_length_21894_cov_6.920692, whole genome shotgun sequence.
ACCESSION   DAOSIM010000009 DAOSIM010000000
VERSION     DAOSIM010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 21894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21894
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2640_length_21894_cov_6.920692"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(405..8576)
                     /locus_tag="PLR47_00895"
     CDS             complement(405..8576)
                     /locus_tag="PLR47_00895"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HOG11518.1"
                     /translation="MNKIYRLIWSKAKEMWLAVSEKVAAGWYRRPLTVGGLALAALLA
                     AGAPARALDPNALPTGGQVVAGQAAIAQAGNTMTVSQQTDRLIAQWNSFNIGRDASVA
                     FQQPGVSSVALNRILDQNPSQIFGSLAANGQVFLLNPAGVFFGPTARVDVGGLVASSL
                     HLANEDFLAGKYSFAGSSTAGAIANQGQIRTAEGGYVAFLSPRIVQEGTITAPRGTVA
                     MAAGEKVDLDFTGDRLVHFTVTQGAIDAQIANKGLIRADDGLVMLTAKAADALTQAVV
                     NNDGVIEAAGLHERGGRILLDAEGGQTTVAGTLDASSAEGRGGRIVATGDRVLIASGA
                     HLTASGATGGGEVLVGGSWQNSDPAVRQAVGTVVETGALLEANATDRGDGGTVVAWSD
                     VTNPGSVTRAYGAFEAKGGPAGGDGGRIETSGHWLDASGAQGGASAPLGRAGEWLFDP
                     YNVTITGATANGAWGGGNPDLWTPSASGSTILNTDINNKLENGTSVTVTTNGGGAEIG
                     DITVGAAVTKASGNADVTLTLQAANSISVDEAISNTGGTGKLHVVLDADNDNGTRDGG
                     GVILLSNDISTGGGNLSFGTGATLNINGVTTLVGGDVYVAGAGARSISTSGGSVDVKG
                     EMIVANTNGLTISTDNGNVRFHGLLNSGNQYTFVDKTGSAGTGTWDDARTEAKNGTGG
                     GSAVNDSYLVTITSRLENAIAVAAAGYKGSWIGAYRPNVASLNWYWADGPEGGAHFFT
                     QISGGGGSATGGYYANFGAGEPNGSTAPGGETRGQFFGTGGQWNDLVNTTTYKASQGG
                     VYDVLGFVRETNLPASPVTVNAGAGTVTFSGAVGTSKALASLNVTAATTAINGGAVIT
                     DSAGVAGGQSYSGNVTLGSAATTLTQRDTPSAFTVAAGKSITNLTGADATLTVKNTGA
                     IVLDAGSAISSAAGKLNTVLWSDTDANGGYIKLNSGSSITTNGGHLWMGGGSGSTAWN
                     GLTVGDGRATGNATQYEGIYIDRGTLSTGGGHAALLGKGHSAASVGGNYSTGVAIGGA
                     TGDTDGTASVNTGAGDLLIDGYAQAAAGAGAGVELAYYGTATTVQSTSGDITIQGDSE
                     SNRGIIFQDNATVAATGGGAVTLEGTARSGNNFGINLDGGNARVLANSGLIRLTGTGS
                     GSGEDLRLGGPIGSVAGGLVENSTSDIEINADSLTAFGANTRFQSTGALTLQPRTAGT
                     TIGIAGGAGTLQLPAGYFTTNFTDGFSGITVGKSDAGLISLGGSALSYNDPLTLKTGN
                     SIFFGTASAVAGNGNALTLWTRAGGNDAADDGVNGSVWMPVGSTVDTGGGDVTIGGGS
                     DPTVGYALGDIGAGSDENNARYRGVTINGTLDAGGGNVSILGRGGNMGSTRGVSIGGA
                     VSTSGDGNITVKGVAKGVSDGLALGDTALAGSSLGTLTAQNGHISLYGTKDTGSNGIN
                     LSTADSRITTNGTGSLTLTATGNIGGTGAVAVGGATTLTAGVSHITLANAANDFTGAV
                     SVVSGNNVSLRDSNALDLGASTIGGNLTLQTGGALTQSGALAVTGTTGITAGADNDIT
                     LNNGANDFTGAVAVVSGNNVSLRDSNALGLGAVSMAGHLTAEALGGDLTIGGNISKTS
                     GTDATAALKATGSIVQNAAASITSTSNKLHTILWSDADNSGSGHILVTDGNTIDTNGG
                     NLVMAGGLDDGANGGTSGDGIPDGYAVSNTANRAGVGIGRPDNTATAATTLQSGGGDI
                     ILRGRSTGAGGEMGVYFAHSGTLAADDGTLTILGESAATHGVELSAWVSGLLNISGRT
                     VSIQGKTSAAGSHGLATSELGGKYASITAGAGGITLYGEHTGAETGGIQMAVNLTATG
                     GGAILIESPDTFWFYNAHNAHSYSAGTGDITIRANGVTNSGSAGTTAFSGTGDLTIEP
                     YSAGTTIGIAGGAGTLQLPAGYFTTNFTDGFSGITVGRSDGTGKITAGAFAVNDALTL
                     RTDSGGIELTGLLNAGANTVTVNSTGSVTDSGSGAIAAGGLALLGAGGNFTLDGTGND
                     VDTLAADTGSVTYVDGDALTIGTVNPTGITATGPVNIATLTGDLTVSQNIVTSDTSNA
                     AIVLNAGKSVAAGTAAGGNIVLSGSPAFTTGAGGRTTLYTGGISGSTGLTALLGSGSG
                     RFRYHSDETATNYTTALGAGSYAIYREQPTVSVTPGSQTITYGDAVPGFTPSYTGFVN
                     GDTGAVLTGSPVWTVGGANSTGGHKVAGIHDVAYASGLGNGLGYAIADAPASTGELTV
                     NKYNLAVSGLSASDKVYDATTTAVLTGTATITPLAGDAVVLGGTPTGAFADKNAGTAK
                     AVTVSGSTISGADAGNYNLLQQAGFTASIAAKTVQLSATKTYDGTTDLTGAVTLATGI
                     AGESLSYTGATALSRNATDNATNCINAITLADGAGGQAGNYRLPALTHDDAPVTITPA
                     ALTVTAKDAAKTYGQTVTFGGTEFTSAGLLNGETIGSVTLASAGASAAAGVTGSPYAI
                     TASNADGGTFTAGNYAITYQDGKLTVRANSQGEAAEAAVSSPQRGMNGTGQESEGTPG
                     ASSTPSLGPGAGSGNIVAASVIAPHTTIGAVLATTTFSTPVTLSATGTTMTLSVAGTT
                     ASGPMTEVGALPIFTQNGGAPPALQGNFLVRESGSALSLTPAGAAGVTVPLPAAGAGE
                     KSAPFTLTQENGLTLQMTASVTADGVLVVSAPDTAGVDVQQAILIGAQVARQALRVEL
                     SSLNAALFVSN"
     gene            complement(8758..10497)
                     /locus_tag="PLR47_00900"
     CDS             complement(8758..10497)
                     /locus_tag="PLR47_00900"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HOG11519.1"
                     /translation="MNPFLHRVFILLALFFHFLFLCGAVGAQPDAGTLLQEQRQLLPT
                     LPDRLPGEGPREEVKAPLADTGSKVIVKAFRFTGRYEGMASEAELLALVARHIGQELG
                     FAELQQVAGRVTQYLREQKGFLLARAYLPRQDVTDGIVEIAILAGRLDGKVRIDLKEP
                     SRISPSLLQGIADRAVPEGSPIRMERIERAVLLMNDLPGVNAQASLERGAVPGSTRLV
                     VNAAEGPLVQGLLSGDNYGDRFTGDLRATGQLSLYDPSGRGDQFSLSLTGAERLFQGR
                     AAYAIPLGASGWTWNAAYTGLDYRLGRELEDLDSRGSADTYATGLSYPLLRSRQASLW
                     SGIGFEYLHLVDKANGDKIRDRKLPVGNLSLSGSFFDGFGGGGLTSANAVVTGGSLDL
                     SGHEVNKANDERGPKTHGGFLRGAYSLARLQRLSRDRSLFGSLRGQFASGNLDSSQKF
                     ILGGPTGVRAYPVGEAAGDEGHVLTVETRYDLPLLPSWAATQLVAFFDGGWVKLHRSP
                     WPGAVASATGKNDYWLSGGGLGLNVGKAGLYSLRFSYAHKIGPNDGKSAAGKDADNRS
                     DDGRFWLQLLLWL"
     gene            complement(10958..11296)
                     /locus_tag="PLR47_00905"
     CDS             complement(10958..11296)
                     /locus_tag="PLR47_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG11520.1"
                     /translation="MGNQEEGNLDRLMRNTVESIKTSLEEAIAHRKGKKTDIKVYTPA
                     KVDVKKVREKTGLTQEKFAATFGISLGTLRHWERGDRAPHGPGLVLLNAIGKEPEAIL
                     RILGSKKRTP"
     gene            complement(11260..11577)
                     /locus_tag="PLR47_00910"
     CDS             complement(11260..11577)
                     /locus_tag="PLR47_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOG11521.1"
                     /translation="MAQIDDSIKGTLENYLAKLERAGIRVEAAYLFGSFAANAQRNES
                     DIDIAIVSPDLTGNRYEERVRLMEFSSDIDTRIEPVPFRPENFVDEDPLAWEIKKKGI
                     LIV"
     gene            complement(11553..11966)
                     /locus_tag="PLR47_00915"
     CDS             complement(11553..11966)
                     /locus_tag="PLR47_00915"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOG11522.1"
                     /translation="MEGVIQLSFDSRKVIAYWRDEAEEDLKVAGHLLEKGDYSYSLFF
                     GHLATEKILKAIYVLKKGEHAPFVHNLVRLATEAGIELSEENKKDLIRITAFHLEARY
                     PDENRSFREKCSKDFSEHELAQIKVIYRWLKSMIP"
     gene            complement(12233..14263)
                     /locus_tag="PLR47_00920"
     CDS             complement(12233..14263)
                     /locus_tag="PLR47_00920"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOG11523.1"
                     /translation="MTTILAIFLIALLISIVLTPLAGKMGLAFGAVDAPGERKVHRLP
                     LPRIGGLAVALSFLAALAIADLVFRTRASDFLALNRQTTFALIGGLVVFGIGFADDFR
                     SIKAPVKLVFQIAGASIAFWGGVRIGSFALLGTGMMFGMVESYILTVFWFLLFINAVN
                     LIDGLDGLAAGISFFASLVMVVLAVMKEDILTAMMFAALGGAVLGFLRYNFNPATVFL
                     GDGGSYGLGYAIACLSIMGSVKSSVGAAMLIPLLAMGVPIFDTILSPLRRFFRGRAMF
                     RPDDGHIHHRLVGMGLSARNAVLIIYGISFGLCLLAIAIVNVRNEVAGLFFVIVGAAA
                     LIVVRKLGYVGYFALDKVYGWFRDVSDVAGLTQERRTFLSLQLDVDKAKTMDELWEDV
                     CKAMEMLHFDRAELRLWKDGQEPDPGLSVKPHSMDDQRAGSPPARRRQEGTLDSRFRG
                     NDKSSEPSVAPAHPPVTPASSVAPAPSSVTPAKAGDQRSGYAPDAAGEDTGPWIPAFA
                     GMTGSTLDSRFRGNDKEKSGNDKEGLDGALPVNQGSEDRQGAGSPPTRGRQEGDGYTT
                     YAGQERRAAKAAPAGRNANIWKKAVEGGSAREWTWVRGFYRRRTDITREDLLKIEFPL
                     FARNGGRELGLLILIKDVRRNPLDYYTFRRLENLRRSLTSALTRLTQNPPVH"
     gene            complement(14609..14830)
                     /locus_tag="PLR47_00925"
     CDS             complement(14609..14830)
                     /locus_tag="PLR47_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11524.1"
                     /translation="MKNRLDYDGEPFLLLEAKLANAEPATALLYFRDRLRIPAVQLTG
                     AGESYRLFGGSEEAKVLVAPAAAWLSLLP"
     gene            complement(14881..15996)
                     /locus_tag="PLR47_00930"
     CDS             complement(14881..15996)
                     /locus_tag="PLR47_00930"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG11525.1"
                     /translation="MIVTGARQAGKTSILRRMFPQASYLSLDLPANAEAANTAPEELL
                     EQYPAPVIIDEIQYAPSLLRHLKYRIDRNRAPGQYLLTGSQVFQLMQGVSESLAGRCG
                     ILNLHTLSRAELLSAGRPIEETSYIFLGGYPELHVGAEAALWFPSYVATYLERDVRNV
                     LRVVDLQDFNRFIRACALRTSQVLNYSDIARDAGISPNTARKWLSLLQASAVVALVEP
                     YWGNRTKRLIKAPKLVFLDTGLAAFLAGFRSAEALFSSPLAGAFWDSHVFGQILRHSA
                     SAGEASVLHYWRTTNGAEVDLVMEEAGGAAIAVECKWKEHPGDADAAGLRALEAAEKD
                     RITGKFILCRTQAAYKLADGTWVMNLPQFLERKTHKR"
     gene            complement(16107..16250)
                     /locus_tag="PLR47_00935"
     CDS             complement(16107..16250)
                     /locus_tag="PLR47_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11526.1"
                     /translation="MKREKYKSRLLGALHETACDLHKLGFIDKRRMREYDLLCVQQRM
                     RQS"
     gene            complement(16240..16524)
                     /locus_tag="PLR47_00940"
     CDS             complement(16240..16524)
                     /locus_tag="PLR47_00940"
                     /inference="COORDINATES: protein motif:HMM:NF018050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOG11527.1"
                     /translation="MTLDFPCASIQSTELAVLPCLGKGKLGSTRALLATNRDNRWFFL
                     FGFEKNERDNITESELDTLKKLAKDLLGLTAGQIAAAVKEGSLVEVEHEA"
     gene            complement(16574..17347)
                     /locus_tag="PLR47_00945"
     CDS             complement(16574..17347)
                     /locus_tag="PLR47_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOG11528.1"
                     /translation="MKHENILGYPVAAVSSEVCVDGILKRIGATVEGSAPLPFWLACL
                     NPHSARMAVTDPAAEKALKSADILIPDGIGVVIASQILGGIIRERITGSDIFRDLSRK
                     LNDQGNTSYFLLGSTEETLAVIKARLTMDFPRIRLAGSYSPPFKEVFGEAENQLMIEA
                     VNRAAPDILWVGMTAPKQEKWIHRNKGKLNVKFIGAVGAVFDFYAGNVKRSSPWFLEH
                     GLEWLPRLLQEPRRLWQRTFVSAPLFLLRVLRQRITGKV"
     gene            complement(17347..18567)
                     /locus_tag="PLR47_00950"
     CDS             complement(17347..18567)
                     /locus_tag="PLR47_00950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG11529.1"
                     /translation="MKILLAHNFYGSSAPSGENTVFQAEAALLRQEGHDVIEFTRHSD
                     EIRERGAVGTLQGALSTPWNPFSAAKLRQVLQHMRPDVLHVHNFFPLLSPAILHATAG
                     LETATVMTLHNYRLFCAAGIPMRAGKPCTLCLNRRSVIPALRFGCYRGSRAATAPMAL
                     MITLHRGIHTWSRHVDAFIALSGFQKSLMNQAGLPETSIHVKPNFYPDPPAALPWDSR
                     EDKIVFIGRLGEEKGVHLLVQAWKQWGADAPPLEIIGDGPLRETLTETAAKEGLEGKI
                     LFLGQLPFAEAQQRLSRVRLLVLPSLCFETFGLVACEAFAFGVPVAASRLGPLPDIVP
                     EGRAGALFEAGNVEDLLRVVRGLWSNQTLLSQMAVGARKEFEEKYTADVNYRTLMQIY
                     EAAIHRRERRHSYN"
     gene            complement(18545..19726)
                     /locus_tag="PLR47_00955"
     CDS             complement(18545..19726)
                     /locus_tag="PLR47_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11530.1"
                     /translation="MYEDNPAASSCIVPPDAFIGLPPSDPRLLVPLASSRIQCLSFRL
                     YSPGSPLGKLFKWCGWLAARTGLLNFAGRLRFAPIDSFQTTNNIQPILDNALFGELKC
                     KWVRMLGKGPVAVALSLGTPDNCRKIAALVFDREATPLAFAKVGCTPQARSLIAHERK
                     TLDVVRTLPLQHATIPMLLGQGTTGPATWLLQSALLTGRPSPVVLQKVHIDFLADFAQ
                     ATTRVVSFHTNGIWKQLQRILKKPVLPIKSGFEVEKPFISELVRRFQASGTEETTKSW
                     PVSAAHGDFAPWNMRLAEGKIALFDWEYFLPEAPVGWDLLYFLFRVDNLIKRKALEQI
                     WSEFHSGAYRNMLDKWEKATGFQVPDRQFLATFVLLSFALDLVPRWVCGQRIDEDSTG
                     T"
     gene            complement(19713..20954)
                     /locus_tag="PLR47_00960"
     CDS             complement(19713..20954)
                     /locus_tag="PLR47_00960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG11531.1"
                     /translation="MKVLVSAYACEPGKGSEPEVGWQWVQQIARFHETWVITRENNRL
                     LIEQNFKSKSNPNLHFVYIDLPPWMRFWKKGRRGVHLYYYLWQIIAYRHAFALSKRVH
                     FDLTHHITFINMYMGPWIALLPVPFVWGPIGANPDIPKQFYQLIGSSGIKDNRLRYAI
                     RKISPLTDPMISLARKKAKLILTINEEVGSRLEETYATKSLSISQNAIDKEILAEKAK
                     SFHYPLKILSVGQLVSIKGYRLSLAAFAKHLSVHPKSTLEIIGTGPQKNELVKFASEL
                     GVFKSVIFLGQISRDKVFQAMQEESNVFFFPSFEGAGMVVIEAMAKGLPVVCLDFGGP
                     GEYVTEECGIKVALTKPEAVIQGLADALSRLASDPALYERLSAGAIKRVREHYLWDRV
                     GDRLNALYEEVRAEEAGRVRG"
     gene            complement(20951..21571)
                     /locus_tag="PLR47_00965"
     CDS             complement(20951..21571)
                     /locus_tag="PLR47_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11532.1"
                     /translation="MIQTMKSALDKANHNATHVLHLRPGLLPPLARLKGKAAIQEGPV
                     LEPHGATPSGPVGSLIRLVYYTFDYILGYWLKIRPMIVTQPTIVIFDRYAYDMALDPR
                     RFRIGLSGCVAGWFAALAPKPDLILCLYAPPEVIMARKRELPMDEIYRQVEALKAFAA
                     QESRAVLISNSGSVDEVRTRVLQVIFDFLSRRWVGSPFASGGVIKQ"
     gene            complement(21668..>21894)
                     /locus_tag="PLR47_00970"
     CDS             complement(21668..>21894)
                     /locus_tag="PLR47_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11533.1"
                     /translation="PKPGIAIIVYEIFTSPRIASLRNTLESLKIPVVLIQSSDMGEKE
                     LALRIVGAVSNAKHNEFLRQKKLPTFRSAL"
BASE COUNT         4015 a         6563 c         6887 g         4429 t
ORIGIN      
        1 gtatcgtcgt catttcaaca aggtcattcc gcgctgccga aggtaaccga tcacgaaaca
       61 gaataaatcg ctcgctgtcc ggaccgaatg cgccggcact tgtggaaagg ttttccaagc
      121 gatcggcgtt gcgatcccgc ccactatcag ggagatgtca tggcagttga tcggctcgtc
      181 aaggagcaaa tgtggtgtca agaacttttt ataggtagtt atcttcccaa gatcattgaa
      241 tatatttttt gcaactgtca aagatccgtt tgacgtctgc tgtggggaat gtctgcgccc
      301 ctgcggggcg ctggcagcag cgctggacga aagtgccgaa cacggtcttt tcgcagacac
      361 gggcgctccc cgggacctct ccagcgatcc cggggcggca gcggtcagtt gctcacgaac
      421 agcgccgcgt tcagggagct cagctcgacc cggagcgcct gtctggccac ctgggcgcct
      481 atgaggatcg cctgctggac gtcgactccg gccgtgtcgg gcgccgagac gaccaggacc
      541 ccgtcggcgg tgaccgaggc agtcatctga agcgtcaggc cgttctcctg ggtcagcgtg
      601 aagggggccg atttttcgcc ggcccctgcg gccggcaggg gcacggtcac tcccgcggcg
      661 cctgcgggcg tcagggagag ggcgctgccg ctctcccgaa ccaggaagtt gccctgcagg
      721 gcgggcgggg cgccgccgtt ctgggtaaag atgggcaagg ctcccacctc ggtcatcggc
      781 ccggaagcgg tcgttcctgc gacagacagg gtcatggtcg tgcccgtggc gctgagcgtg
      841 accggtgtgg agaaggtcgt tgtcgcgagg actgcgccga tcgtcgtgtg cggggcgatg
      901 accgatgccg cgacgatgtt gccactgcct gcgccgggac ccagggaggg ggtggaggag
      961 gcgccgggcg ttccctccga ctcctggcca gtgccgttca tcccccgctg gggactggaa
     1021 acggcagctt cggcggcctc tccctggctg ttggcgcgaa cggtcagttt gccgtcctgg
     1081 taggtgatgg cgtagtttcc ggcggtgaag gtaccgccgt cggcgttgct cgcggtgatc
     1141 gcgtaggggg agccggtcac gccggcggcg gccgaagcgc cggcgctggc gagggtgacg
     1201 gaaccgatgg tctccccgtt cagaaggccg gcggaggtga actccgtgcc gccgaaggtg
     1261 acggtctggc cgtaggtctt ggccgcgtcc ttggccgtca ccgtcagcgc tgcgggagtg
     1321 atggtcaccg gggcgtcgtc atgggtcagt gcgggcaacc gatagttgcc ggcctgtccg
     1381 cccgcgccgt cggccagggt gatggcgttg atgcagttcg tcgcgttgtc ggtggcgttc
     1441 cgggaaaggg ccgtcgcacc cgtgtagctc agggactcgc cggcgattcc ggtggcgagc
     1501 gtcacagcgc cggtgagatc ggtcgtgccg tcgtaggtct tggttgccga taactgcacc
     1561 gttttggcgg cgatgctggc ggtgaacccg gcctgctgga ggaggttgta gtttccggca
     1621 tcggcgccgg agatcgtgct tccggagacg gtgacggcct tagcggtgcc cgcgttcttg
     1681 tcggcaaagg cgcctgtggg ggtgccgccg aggacgaccg catcgccggc caggggggtg
     1741 atggttgcgg tgccggtcag gacggccgtc gtggtggcgt cgtagacctt gtcggaggcg
     1801 ctcaaaccgc tgaccgccag gttgtacttg ttgaccgtca gttcgccggt gctggccggg
     1861 gcgtcggcga tggcataccc gagaccgttg cccaggccgg aggcgtaggc cacgtcatgg
     1921 atgccggcga ccttgtggcc cccggtggag ttcgcacctc ccaccgtcca gacggggctg
     1981 cccgtcaaca ccgcgccggt gtcgccgttg acgaagccgg tataggaggg ggtgaagccc
     2041 ggcacggcat cgccgtaggt gatcgtttgg cttcccgggg tgacggagac ggtcggctgt
     2101 tcccggtaga tggcgtagct gcccgcaccc agggccgtgg tgtagttcgt cgccgtctcg
     2161 tcgctgtggt agcggaagcg gccgctgccc gagccgagca gggccgtcag gcccgtgctc
     2221 ccggagatgc cgccggtgta gagggtggtt cggccaccgg cgccggtggt gaaggcgggc
     2281 gaaccggaaa ggacgatgtt gccgcccgcg gcggtgccgg cggcgacgct cttgcccgcg
     2341 ttgaggacga tggctgcgtt ggaagtgtcg cttgtcacga tattctgcga gacggtcagg
     2401 tcgcccgtca gggtggcgat gttcaccggt cccgtggcgg tgatgcccgt ggggttcacg
     2461 gtgccgatgg tcagggcgtc gccgtccacg taggtgacgc tccccgtgtc ggcggccagg
     2521 gtgtccacgt cgttgcccgt tccgtcgagg gtgaagttcc cgcccgcccc caggagggcg
     2581 aggcccccgg cggcgatcgc gccgctgccg ctgtcggtca ccgacccggt gctgttgacg
     2641 gtcacggtgt tggcgcccgc gttgagcaga cccgtcaact cgatcccgcc gctgtcggtc
     2701 cgcagggtca gggcgtcgtt caccgcgaag gccccggcgg tgatcttgcc ggtgccgtcg
     2761 ctgcgtccca cggtgatgcc ggagaagccg tcggtgaagt tggtcgtgaa gtagccggcc
     2821 ggcagttgca gcgtcccggc gccgcccgcg atgccgatgg tggtgccggc gctgtagggc
     2881 tcgatggtca ggtcgcccgt tccgctgaat gcggtcgtgc cggcgctgcc cgagttggtc
     2941 accccgttgg cgcgaatggt gatgtcgccg gttcctgcgc tgtagctgtg cgcgttgtgg
     3001 gcgttgtaga accagaaggt gtcgggcgat tcgatgagga tggctccccc gcccgtggcg
     3061 gtgaggttga ccgccatctg gatgccgccg gtctctgccc cggtgtgctc gccataaagg
     3121 gtgatgcccc cggctcccgc tgtaatcgat gcgtatttgc cgcccagttc ggaggtggcc
     3181 aggccgtgag agcccgcggc agacgttttg ccctggatac tgactgtgcg tcccgaaata
     3241 ttcagcagtc cgctcaccca ggccgacagt tccacgccgt gggttgccgc cgattctccg
     3301 agaatcgtca gggtgccgtc gtccgcagcg agggtgccgc tgtgggcgaa gtagacgccc
     3361 atctcgccgc ccgctcccgt ggaacggccg cgaaggatga tatccccgcc gccggactgc
     3421 agcgtggtgg cagccgtcgc ggtgttgtcg gggcggccga tgccgacgcc cgcccggttg
     3481 gccgtgttcg agacggcata gccgtcggga atgccgtcgc ctgacgtccc gccgttggcg
     3541 ccgtcgtcca gaccgccggc catcacaaga ttcccgccgt tggtgtcgat ggtgttgccg
     3601 tccgtcacca ggatgtggcc gctgccggag ttgtcggcgt cggaccagag gatcgtatga
     3661 agcttgttgc tcgtcgaggt gatggaggcg gcggcgttct ggacgatgct gccggtcgcc
     3721 ttcagagcgg ccgtggcatc cgttccgctg gttttgctga tgtttccgcc aatggtcaaa
     3781 tcgccgccga gcgcttcggc cgtcaggtgg cccgccatgc tcacggcccc caggccgagg
     3841 gcgttgctgt cccgcaggct gacgttgttg ccggagacca cggcaacggc gccggtgaag
     3901 tcgttggccc cgttgttcag ggtgatgtcg ttgtccgcgc ccgccgtgat gccggtggtt
     3961 cccgtgaccg ccagggcgcc gctctgggtg agcgccccgc cggtctggag ggtcaggttg
     4021 ccgccgatgg tcgaggcgcc caggtccagg gcgttgctgt cccgcaggct gacgttgttg
     4081 ccggaaacca cggagaccgc accggtgaaa tcgttggccg cgttggcgag cgtgatgtgg
     4141 gaaacgcctg cggtgagcgt ggtggcgccg ccgacggcga ccgcgccggt cccgccgatg
     4201 ttgccggttg cggtcagggt cagcgacccc gtaccgttgg tggtgatccg gctgtccgcc
     4261 gtggacaggt tgatcccgtt gctccccgtg tccttggtgc cgtagaggga aatgtggccg
     4321 ttttgggcgg tcagggtgcc gagcgatgac ccggcaagcg cggtgtctcc cagcgccaga
     4381 ccgtctgaaa cgcccttcgc cacgcccttg acggtgatgt tcccgtcgcc gctggtgctg
     4441 acggcgccgc cgatgctcac cccgcgggta ctgcccatat tcccgccccg tcccaggatg
     4501 gacacgttgc ccccgccggc gtcgagcgtc ccgttgatgg tgacgccccg gtaccgggca
     4561 ttgttttcgt cggagccggc gccgatatcc ccgagggcgt aaccgaccgt cgggtccgat
     4621 cccccgccga tggtgacatc gccgccgccg gtatccacgg tggagcccac cggcatccag
     4681 acggaaccgt tcaccccgtc atcggcggcg tcgtttcctc cggcccgggt ccagagggtc
     4741 agggcattgc cgttgccggc cacggcggag gcggtgccga agaagatgct gttgccggtc
     4801 ttcagagtca gcggatcgtt gtaggagagg gcggaaccgc cgaggctgat caaaccggcg
     4861 tccgatttgc ccacggtgat cccggagaag ccgtcggtga agttggtcgt aaagtagccg
     4921 gccggcagct gcagcgtccc ggcgccgccg gcgatgccga tggtggttcc ggcggttcgg
     4981 ggctggaggg tcagggcgcc cgtactttgg aatcgcgtat tggcgccgaa agcggtcagg
     5041 gaatcggcgt tgatttcgat gtcgctggtc gaattctcca ccaggccgcc ggcgacggat
     5101 ccgatcggac ccccgaggcg caggtcctcc ccggagcccg atccggtgcc ggtcagccgg
     5161 atcagtccgc tgttcgccag gaccctggcg ttgccgccgt ccaggttaat cccgaaatta
     5221 ttcccggacc ttgccgtgcc ctccagcgtc acggcgccgc ctccggtcgc cgcgacggtc
     5281 gcgttgtcct ggaagatgat cccgcgattg ctctcggagt cgccctggat ggtgatgtcg
     5341 ccgctggtgc tctgcacggt ggtggccgtg ccgtagtagg ccagctcgac gccggcgccg
     5401 gccccggcgg ccgcctgggc gtagccgtcg atcagaaggt cgccggcgcc ggtgttgacc
     5461 gatgccgtgc cgtccgtatc cccggtggcc ccgccgatgg cgaccccggt ggaatagttg
     5521 ccgccgaccg aggccgccga atggcccttc cccagcaggg cggcatgtcc gccgccggtc
     5581 gacagggtgc cgcggtcgat gtagatcccc tcgtactggg tggcgttccc ggtggcgcgg
     5641 ccgtcgccca cggtcaggcc gttccaggcg gtcgacccgg agccgcctcc catccagagg
     5701 tggccgccgt tggtggtgat gctgctgccg ctgttcagct tgatgtagcc cccgttggcg
     5761 tccgtgtcgg accaaaggac cgtgttgagc ttgcccgcgg cggagctgat ggcgctgcct
     5821 gcgtccaaaa cgatggcgcc cgtgttcttg acggtcaggg tggcgtcggc gccggtcaag
     5881 ttggtgatgc ttttcccggc ggccaccgtg aaggccgagg gggtatcgcg ctgggtcagg
     5941 gtcgtggcgg cgctgcccag ggtgacgttg ccggaatagc tctggccgcc ggcgacgccc
     6001 gccgagtcgg taatgaccgc gccgccgttg atcgccgtgg tggcggcggt gacgttcagg
     6061 gaggcgaggg ccttgctggt ccccacggcg ccggagaatg tgaccgtgcc ggcgccggcg
     6121 ttgaccgtga ccggcgaggc gggcaggttg gtctcgcgga cgaagccgag gacgtcatag
     6181 acacctccct ggctcgcttt ataagtggtg gtattgacga ggtcgttcca ctggccgccc
     6241 gtcccgaaaa actgcccccg cgtctcgccg ccgggggcgg tgctgccgtt cggctctccg
     6301 gcgccgaagt tggcgtagta gcctccggtc gcgcttcccc cgccgcccga gatctgcgtg
     6361 aagaaatgag ccccgccttc ggggccatcg gcccaatacc agttcagaga agccacgttc
     6421 ggccggtagg cgccgatcca ggaccccttg tacccggcag cggccacggc aatcgcgttt
     6481 tccagacgcg aggtgatggt gaccaggtag ctgtcgttga cggcggagcc gcccccggtg
     6541 ccgttcttgg cctccgttcg ggcgtcatcc catgtccctg tccccgcact gccggtcttg
     6601 tcgacaaagg tatactggtt cccggagttg agcagtccgt ggaaccggac attgccgttg
     6661 tcggtggaaa tcgtgaggcc gttcgtattg gcgacgatca tctcgccctt gacatcgacg
     6721 ctgccgccgc tggtggaaat ggatcgcgct ccggccccgg ccacgtagac gtcgccgccg
     6781 accagggtgg tgacgccgtt gatgttcagg gtggcgccgg tgccgaagct gaggttgccg
     6841 ccgccggtgc tgatgtcgtt gctcaggagg atgacgccgc cgccgtcgcg cgtgccgttg
     6901 tcgttgtcgg catcgaggac cacgtggagc ttgcctgtgc cgcccgtgtt gctgatcgcc
     6961 tcgtccaccg agatggagtt ggctgcctgg agggtgaggg tcacgtcggc attgccgctc
     7021 gccttggtga cggcggcccc gacggtgatg tccccgatct cggcgccccc gccgttggtc
     7081 gtgacggtga cgctcgtccc gttttccagc ttgttgttga tgtccgtgtt gaggatcgtc
     7141 gagccgctgg cgctcggggt ccagaggtcg gggttgccgc cgccccaggc gccgttggcc
     7201 gtggcgccgg tgatggtcac gttgtagggg tcgaagagcc actcgcctgc ccggccgagg
     7261 ggggcggagg cgccgccctg cgccccgctg gcgtcgagcc aatggcccga ggtctcgatc
     7321 cggccgccgt cgccccctgc ggggccgccc tttgcctcga aggcgccata ggcccgggtg
     7381 acggagccgg ggttcgtcac atcggaccag gcgaccacgg tgccgccgtc gccgcggtcg
     7441 gttgcgttgg cttccagcaa agctccggtt tcgacgaccg tcccgacggc ctggcgaacg
     7501 gcgggatccg agttctgcca actccctccc accaggacct cgccgccgcc ggtcgcgccg
     7561 gaggcggtca ggtgtgcgcc tgaagcaatc agcacccggt cccccgtggc cacgatccgg
     7621 ccgccccggc cttccgctga ggacgcgtcc agggtgccgg cgacggtggt ttgcccgccc
     7681 tcggcatcga gcaggatccg gccgccccgt tcgtgcaggc ctgcggcctc gatcacgccg
     7741 tcgttgttga cgaccgcctg ggtcagggca tcggccgctt tcgcggtcag catcaccagt
     7801 ccatcgtcgg cccggatgag ccctttgttg gcaatctggg cgtcgatcgc cccctgggtg
     7861 acggtgaaat ggacgagccg gtctccggtg aagtcgagat ccaccttctc gcctgcggcc
     7921 atggcgaccg tgccgcgcgg ggccgtgatc gtcccctcct ggacgatcct gggcgacagg
     7981 aaggccacat aaccgccctc cgccgtgcgg atctgcccct ggttggcgat cgcgccggcg
     8041 gtgctgctgc cggcaaagct gtattttccg gccaggaagt cctcattggc gagatggagc
     8101 gacgatgcga cgaggcctcc cacgtccacc cgggccgtgg ggccgaaaaa gacgccggcg
     8161 gggttgagga ggaagacctg gccgttggcc gcgagagacc cgaagatctg cgacgggttc
     8221 tggtccagaa tgcggttcag ggccacgctg gagacccccg gctgctggaa ggccaccgag
     8281 gcatcgcggc cgatattgaa gctgttccac tgggcgatca gtcggtcggt ctgctggctg
     8341 accgtcatcg tgttgccggc ctgggcgatg gcggcctggc cggcgacgac ctggccgccg
     8401 gtgggcaggg cgttcgggtc cagggcccgg gccggcgccc ctgccgcgag cagtgcggcc
     8461 agggccaggc cgcccacggt cagggggcgg cggtaccagc cggcggccac cttctcggaa
     8521 acggccagcc acatctcttt cgctttggac cagatcaggc ggtagatctt gttcatcgtg
     8581 cattcctctt gttatctcta tggagcggtg aaaggggcgt tccttcgtcc cttgtttcag
     8641 ctttttccgc tttccgggtc cgggatgacc cggcgtgacg ctctgcctgc cgccggtcgg
     8701 ggcgaggata ccggggaatt gacggcgaga cgtcaaaaat gggcttgcga tccccggtca
     8761 cagccagagc aggagttgaa gccagaagcg gccgtcgtcg ctgcgattgt ctgcgtcctt
     8821 gccggcagcg cttttgccgt cgttgggacc gatcttgtgg gcataggaga accgcaggct
     8881 gtagagtccc gccttgccca cattgaggcc cagaccgccc cccgaaagcc agtagtcgtt
     8941 tttccccgtg gcgctggcga cggcccccgg ccagggactg cggtggagtt tgacccagcc
     9001 gccgtcgaag aaagccacca gttgggtcgc cgcccaggag gggaggaggg gaaggtcgta
     9061 gcgggtctcc acggtgagga cgtgcccctc gtcgccggcg gcttcgccca cgggataggc
     9121 tcggacgccc gtggggcctc cgagaatgaa tttctgagac gaatcgaggt tgcccgaggc
     9181 gaactggccg cggagcgagc cgaagagcga gcggtcgcgg ctgaggcgct ggaggcgggc
     9241 cagggaataa gcaccacgca ggaacccgcc gtgggtcttg gggccgcgct cgtcgttcgc
     9301 cttgttgacc tcgtggccgg agagatcgag gctgccgccg gtgacgacgg cgttcgcgct
     9361 ggtcaaccct ccgcctccga agccgtcgaa gaaactgccg ctgagggaga gattccccac
     9421 cgggagcttc cggtcgcgga tcttgtcgcc gttggccttg tccacgagat gcaggtattc
     9481 gaagccgatg ccggaccaga ggctggcctg gcgggaacgg agcagaggat aggagaggcc
     9541 ggtggcgtag gtgtcggcgc tgccccggga gtcgaggtcc tccagttccc tgcccagcct
     9601 gtagtcgagg ccggtatagg cggcgttcca ggtccagccg ctggcgccca gggggatggc
     9661 gtaggccgcc cggccctgga agagccgctc ggcgccggtc agggagaggc tgaactgatc
     9721 gccccgaccg ctggggtcgt agagggaaag ctgacccgtg gcccgcaggt ccccggtgaa
     9781 gcgatcgccg tagttgtcgc cggagaggag tccttggacc agcggcccct cggcggcatt
     9841 gaccaccagc cgcgtcgaac cgggaacggc cccccgttcc agggatgcct gggcgttgac
     9901 gccgggcagg tcgttcatca gcaggaccgc ccgctcgatc cgctccatgc ggatcgggct
     9961 gccttccgga acggcccggt cagcgatgcc ctggaggagg gaaggactga tccgggaggg
    10021 ctccttgaga tcgatgcgca ccttgccgtc gagccggccg gcgaggatgg cgatctcgac
    10081 gatcccatcc gtcacgtcct gccggggcag ataggcccgg gcgaggagaa agcccttttg
    10141 ttcccgcagg tattgggtaa cccggcccgc cacctgctgc aactcggcaa aaccgagttc
    10201 ctggccgata tggcgagcca ccagggccag cagctccgcc tcgctggcca tcccctcata
    10261 gcggccggtg aagcggaatg ccttgacgat caccttgctc cccgtgtcgg cgagaggggc
    10321 cttgacctcc tcgcgcggcc cctcccccgg gagacgatcg gggagggtgg gcagcaattg
    10381 ccgctgttcc tggaggaggg taccggcgtc cggctgggca ccgacggcgc cgcagaggaa
    10441 caggaaatgg aagaagagag ccaggaggat aaagacgcgg tgcaggaatg gattcatctc
    10501 cttgtaacct caaattatgc ggcagggccg cctttcaaac cctttgcaac ccgaaaatcc
    10561 gacttgaaaa acgtgatcaa gcaagttttt gcctgccaaa gcagtcatga acaggtccat
    10621 cgggaccggt tttcgccggc gatcctctgc aattcatcca cccgtttccc tcacgctggt
    10681 aaatggcgga ggaagatatc aaatagatcg tagtatgtcg atgaattttc gatatactat
    10741 gcgagaacca taatctccgc ccttctcctc aatatccgtc cggtcgaagt gaggcggtcg
    10801 gcaagttccc aaagtcatcc tcgccaagga tattcgcttg acttcggctg tcaggaaaga
    10861 tcgccgtccc ccgcgctcga cgaggagatc cacttccata tgcataataa taccccgcag
    10921 caagctgcgg ggtaagcgat tgtaggcgtg ggataattta aggagtcctt tttttactcc
    10981 ccaagattct caaaattgct tccggttctt tcccgattgc gttgagcaga acaagccccg
    11041 gaccgtgcgg tgcgcggtcg ccgcgctccc agtgacgcag tgttccaaga ctgatcccga
    11101 aggttgcggc gaatttttcc tgggtgaggc cggttttttc tcgcaccttc ttcacgtcga
    11161 ccttggcggg cgtgtaaacc ttgatgtcgg tcttttttcc tttccggtgg gcaatggctt
    11221 cttccaggct cgtcttgata ctttcaacgg tgttgcgcat cagacgatca agattccctt
    11281 cttcttgatt tcccatgcga gggggtcttc atcgacaaaa ttctcggggc ggaagggcac
    11341 cggttcaatt ctcgtatcta tatccgagga gaattccatt aagcggaccc tctcctcgta
    11401 acgattacct gtcaggtccg gcgatacgat cgcgatatcg atatcactct cattcctctg
    11461 tgcattggct gcaaaagacc cgaaaagata agccgcctca actcgaatgc ccgctctttc
    11521 cagcttcgcc aggtagtttt cgagcgtgcc ttttatggaa tcatcgattt gagccatcga
    11581 tagatcacct tgatttgcgc cagttcatgt tccgaaaaat ctttgctgca tttttctcga
    11641 aaagacctgt tctcatccgg ataccttgct tcaaggtgaa aggcggtaat cctgatcaag
    11701 tcttttttgt tttcttcaga caactcgatc ccggcctctg ttgccaacct taccagattg
    11761 tgaacaaacg gcgcatgctc tccttttttc aaaacgtaaa tcgcctttag aattttttct
    11821 gtcgcgagat gcccgaaaaa aagagagtat gaatagtctc ccttttccaa gaggtgacct
    11881 gcaactttca ggtcctcttc ggcttcatcc ctccagtacg cgattacctt gcgagaatca
    11941 aacgacaatt ggattacccc ttccaaatct actcgtatgc ttccacaatg cagtcacgcg
    12001 ggtaaatata atccaatgga ttacgtgaag tcaagatttt taagatagtc atccttgact
    12061 tcggctgtca ggaaagatcg ttttgcaggg cttgccagcc catgacccgg atccctttgt
    12121 ggcgatagga ttcagcgcta caccaagaag ggattcacat tccggatact tcttcctgtc
    12181 attcctgcga aagcgggaat ccagtgtctt tgagaatttg tttctttgcg gatcagtgaa
    12241 caggcgggtt ttgcgtgagg cgggtgaggg cggaggtgag gctgcggcgg aggttctcca
    12301 gccggcggaa ggtgtagtag tccagggggt tcctgcggac gtccttgatc aggatgagga
    12361 gacccagttc gcggccgccg ttcctggcga agagggggaa ttcgattttg aggaggtctt
    12421 ctctcgtgat atcggtgcgg cggcggtaga aaccgcgcac ccacgtccat tcccgggccg
    12481 atcctccttc gacggccttc ttccagatat tcgcattccg gccggccggc gcggcttttg
    12541 cggcccgccg ctcctgcccg gcataagtcg tgtacccgtc cccttcctgt cgtccccgcg
    12601 taggcgggga cccagcgccc tgcctatcct cggaaccctg gtttacaggc agcgcgccat
    12661 caagtccctc tttgtcattc ccgcttttct ctttgtcatt cccgcggaag cgggaatcca
    12721 gtgtacttcc tgtcattccc gcgaaagcgg gaatccaggg tcctgtgtct tcgcctgcag
    12781 catccggcgc gtaaccagag cgctggtccc ccgctttcgc gggggtgaca gaagaaggcg
    12841 cgggggcgac agaagacgct ggggtgacag gagggtgtgc cggtgcgacg gagggttcgc
    12901 tgcttttgtc attcccgcga aagcgggaat ccagtgtccc ttcttgtcgt ctccgcgcgg
    12961 gcggggaccc agctctttga tcatccatgg aatgcggctt cacggagagg ccgggatccg
    13021 gttcttgccc gtccttccac agcctcagct ccgcccggtc gaagtgcagc atctccatgg
    13081 ccttgcagac gtcctcccac agctcgtcca tggtcttggc cttgtccacg tccaactgaa
    13141 ggctgaggaa cgtccgccgc tcctgcgtca ggccggccac gtcggacacg tcccggaacc
    13201 agccgtagac cttgtccagg gcgaaatagc cgacgtagcc cagcttccgc acgacgatga
    13261 gggccgccgc accgacaatc acgaagaaga ggccggccac ctcgttccgg acgttcacga
    13321 tggcgatcgc caggagacac aagccgaagg agatgccgta gatgatcagg acggcgttgc
    13381 gcgccgagag acccatgccc accagacggt ggtggatgtg tccgtcgtcg ggacggaaca
    13441 tcgcccggcc gcggaagaag cgccgcaagg gggagaggat cgtgtcgaag atgggtacgc
    13501 ccatcgcaag cagcgggatc agcatcgccg cgccgacgga gctcttcacg gaacccatga
    13561 tcgacaggca cgcgatggcg tagcccaggc cgtagctccc gccgtcgccc aggaagaccg
    13621 tcgccgggtt gaaattgtaa cgcaggaagc ccaggaccgc cccacccagg gcggcgaaca
    13681 tcatggccgt caggatatct tccttcatga cggccaggac caccatgacc aggctggcaa
    13741 agaaggaaat cccggcggcc agcccgtcca ggccgtcgat gagattgacg gcgttgatga
    13801 agaggaggaa ccagaagacg gtgaggatgt agctctccac catgccgaac atcatgccgg
    13861 ttcccagaag ggcaaagctg ccgatccgga ctccacccca gaaggcgatc gatgcgccgg
    13921 cgatctggaa gacaagtttg accggcgcct tgatgctgcg gaagtcgtcg gcaaagccga
    13981 tgccgaaaac gacaagaccg ccgatcaggg caaaggtcgt ctggcggttc agtgcaagga
    14041 aatcagaggc gcgagtgcgg aagacgagat cggcgatcgc cagtgctgca aggaaagaca
    14101 gtgccaccgc gaggccgccg atgcgcggga ggggaagcct gtggaccttc cgctcgccgg
    14161 gggcatcgac ggcaccgaaa gcgagaccca tctttcccgc aagcggtgtg agcacgatcg
    14221 aaatcaggag ggcgatcagg aatatggcca gtatcgtcgt catttattct ctgtcatgcc
    14281 cgcgcaggcg ggcatccagt gtcccgagtc tttgtctgcc gcgtccagcg agtaaccagg
    14341 gcgctgggtc cccgcgttcg cggggacgac agaaggtttt ttgtcctcgt cattcccgct
    14401 tccctgttgc gcgggccccg gcaacctctc tctttgtcgc ccccgcgtag gcggggaccc
    14461 agccagcgaa gatctcggat ctgcatctca gtacacattc ttaacaattt tttaagaaaa
    14521 caggaacagc ccctctatgg gtcagcatgg cttgtcaaag cgccttgggg ttgcaagtgg
    14581 atcggcaccg taatggtgaa aagctgtact aaggcaggag ggacagccac gccgcggccg
    14641 gggccacgag gactttggcc tcctccgacc cgccgaacag ccggtatgat tcccccgcgc
    14701 ccgtaagttg caccgccgga atacggagcc ggtcccggaa ataaagcaaa gcggtcgccg
    14761 gttccgcgtt cgccagcttg gcttcgagga gcaggaaggg ctcgccgtcg tagtcaagac
    14821 gatttttcaa tgaggtgaaa aatacgacga agcagagata aagcaggatc aggtctattt
    14881 ttagcgttta tgggtctttc tctcgaggaa ctgcgggaga ttcatcaccc acgtgccatc
    14941 ggcgagcttg taggccgcct gcgttcggca caggatgaat ttccccgtga tgcgatcctt
    15001 ttccgcggct tccagagccc gcaggccggc tgcatccgcg tcgccggggt gctccttcca
    15061 tttgcactcg accgcgatgg ccgcgccccc cgcctcctcc atgaccaggt cgacttccgc
    15121 gccgttggtg gtacgccagt aatgcaagac cgatgcttct cccgccgatg cactatgccg
    15181 gaggatctgt ccgaagacat gggaatccca gaaagcgccc gccaggggag aggaaaacaa
    15241 cgcctccgcg gaacgaaaac cggccagaaa ggctgcgagg ccggtgtcca ggaaaaccag
    15301 tttcggggcc ttgatcaatc gcttggtccg gtttccccaa tagggctcga caagagcgac
    15361 aacagccgac gcctgcagga ggctcagcca cttgcgggcg gtgttgggcg agatccccgc
    15421 gtccctcgcg atgtcggagt agttgaggac ttgagacgtc cggagcgcgc aggcacggat
    15481 gaaccggttg aaatcctgaa ggtcgacgac gcgcaaaaca ttccgcacgt cccgttccag
    15541 ataggtggcc acgtaggagg ggaaccacag cgcggcctct gcccccacat gcaactccgg
    15601 atatccaccc agaaagatat aggatgtctc ttcgatcggc cggccggcgg acagcaattc
    15661 ggccctggaa agggtatgca gattcaggat gccgcaccgc ccggcaaggc tctccgaaac
    15721 gccctgcatg agctggaaca cctgggaccc cgtcaggagg tactgtccgg gagcccgatt
    15781 gcggtctatg cggtatttga ggtgcctcag cagggaaggg gcgtattgga tttcgtcgat
    15841 gatgaccggc gcgggatact gctccagcag ctcttccggt gcggtgttcg ccgcctccgc
    15901 attcgccgga aggtcgaggc tgagataaga cgcttgcggg aacattcggc gcaggatcga
    15961 tgtctttcct gcctggcggg cgcctgtgac gatcacggcc gggaattgcc ctgccagccg
    16021 ccggagcagt tcttcagaat gtcgcggaat ccacatgcag ataatatgca atagcgatgc
    16081 aaatcatgca agagaaaagt agtcagtcag ctttggcgca tccgctgctg gacacacagc
    16141 aggtcatact cgcgcattct tcgcttatcg atgaatccca gtttatgcaa gtcacaggcg
    16201 gtttcatgaa gagcccccaa aaggcggctc ttgtactttt cacgcttcat gttcgacctc
    16261 caccaaagaa ccttctttca cggcggcggc aatttgtccc gccgttaaac caagaaggtc
    16321 ctttgcaagc ttcttcaaag tgtccaattc actttccgtt atgttatccc gttcattttt
    16381 ctcaaagccg aagaggaaaa accagcggtt atctcgattc gttgccagca atgctctcgt
    16441 gctgccaagt tttccctttc ccaggcaagg caatacggct aactctgtac tctggattga
    16501 tgcacacggg aaatcgagtg tcattcttgc cgtcatcgcg aggaggccgg caggccgacg
    16561 cggcgatctc tccttatacc ttccccgtaa tcctctgcct cagaaccctc aacaaaaaca
    16621 gcggcgccga aacgaaggtc cgttgccaaa gccgccgcgg ttcctgcaac agcctcggca
    16681 gccactccag gccgtgttcc agaaaccatg gactcgaccg tttcacattg cctgcataga
    16741 aatcgaaaac ggcgcctacg gcgccgataa acttcacatt cagcttgccc ttgttccgat
    16801 ggatccactt ctcctgcttg ggggcagtca ttcccaccca gagaatatcc ggtgcggccc
    16861 gattgactgc ctcgatcatc agttggttct ctgcttcgcc gaacacttct ttgaacggcg
    16921 gggaataaga acctgcgagg cgaatgcgag gaaagtccat ggtgagcctg gccttgatta
    16981 ccgccagtgt ctcctccgtg gaccctagga ggaaatagga agtattccct tggtcgttca
    17041 gtttgcgact caggtcccgg aagatgtcgc tgcctgtaat cctttcacgg attattccgc
    17101 caaggatctg ggaagcaatg acaacgccaa ttccgtcggg gatgaggatg tcagctgatt
    17161 tcagtgcctt ttcagctgca ggatcggtaa cagccattct agccgagtgc ggattcaagc
    17221 aggccagcca aaagggcaat ggcgcagatc cctcgactgt tgcaccgatc ctcttcagga
    17281 tgccgtcgac gcagacttcc gatgacacgg ccgcaaccgg ataacccagg atgttctcgt
    17341 gcttcatcaa ttatagctgt gccgtctttc ccgtctatgg atggccgctt cgtagatctg
    17401 cattagtgtc cggtaattga cgtctgccgt atacttttcc tcgaattctt ttcgtgctcc
    17461 aacagccatc tgggaaagca gggtctgatt tgaccagagg ccgcggacta cgcgaagcag
    17521 gtcctccaca ttgcccgcct cgaaaagcgc cccggcgcgc ccctcgggca cgatatcggg
    17581 cagcggaccg agtcgagacg ccgccaccgg aacgccgaag gcaaaggcct cgcacgccac
    17641 cagcccgaaa gtttcaaaac atagtgaagg caaaacgagc aggcgtacgc gggagagccg
    17701 ctgctgcgct tctgcaaaag gcaattgccc caggaagaga atcttgcctt ccaatccttc
    17761 tttcgccgct gtttctgtca acgtttccct gagggggcca tcgccgatga tctccagagg
    17821 cggcgcatca gcgccccatt gtttccaggc ttggaccagc agatggacgc ctttttcctc
    17881 gcccagacgg ccgataaaaa cgattttgtc ctcccgacta tcccagggga gtgcggctgg
    17941 aggatcggga tagaaattag gtttgacgtg aattgaggtt tcgggcagac ccgcttggtt
    18001 catcaagctt ttctgaaagc cgctcaaggc gatgaaggca tccacatggc ggctccaggt
    18061 atgaatgccc cggtgcagtg ttatcatcaa tgccatcggc gccgtagcag ccctgctgcc
    18121 acggtaacac ccgaagcgca gagccgggat cacacttctt cgattcagac acagtgtgca
    18181 gggcttaccg gcgcgcatag gaatgcccgc agcacagaag aggcggtagt tgtgcagtgt
    18241 catcaccgtg gccgtttcca aaccggcagt cgcgtgaagg atggctgggg acagcaaggg
    18301 aaagaagttg tgcacgtgaa gcacgtccgg ccgcatatgc tgcagtactt gtcgcagctt
    18361 tgcggcactg aaggggttcc aaggcgtgga cagggcgccc tgaagagttc caaccgcccc
    18421 ccgttcccgg atctcgtcgc tgtgcctggt gaactcgata acgtcatgcc cctcttgtct
    18481 cagcagcgcc gcctcggcct gaaacactgt attttcgcca ctgggggccg aagagccgta
    18541 aaagttatgt gccagtagaa tcttcatcaa tcctttgccc gcagacccac ctcgggacga
    18601 gatcgagagc gaaggaaagc aggacaaatg tagccaggaa ctgccggtcc ggcacctgaa
    18661 agccggtcgc tttttcccat ttatccagca tattccggta ggcgccggag tgaaattcag
    18721 accatatttg ttcaagagct tttcttttta tcagattgtc aacccgaaaa agaaaataga
    18781 gtaaatccca gccgacgggt gcctcaggca gaaaatattc ccagtcaaac agggcgattt
    18841 taccctccgc cagtctcata ttccagggtg cgaagtcgcc gtgagcggct gaaacaggcc
    18901 acgatttcgt cgtctcttcg gtccctgacg cctgaaacct acgaaccagt tcggagatga
    18961 aaggcttttc gacttcaaac ccggatttga tcggaagaac cggctttttc aagatgcgct
    19021 gtagttgttt ccaaataccg tttgtatgga aagacaccac acgtgttgtc gcttgagcga
    19081 agtcggccag gaaatcgata tgcactttct gcaaaacgac aggcgatggt cttcccgtca
    19141 gtagggccga ttgcagtagc caggtggccg gtcctgttgt gccctgtcca agcagcatcg
    19201 ggatagtggc gtgttgcagt ggcagcgttc tgaccacgtc gagcgtcttc cgttcgtgtg
    19261 caatcaaact tctggcctgc ggtgtgcacc cgactttggc aaaggcaagc ggcgtcgcct
    19321 ctcggtcaaa cacaagtgcc gcgattttcc ggcaattatc gggtgtcccc agcgacaggg
    19381 cgacagcaac cggccccttg ccgagcatcc gtacccattt gcatttcaac tctccaaaca
    19441 gcgcattatc caaaatcggt tgaatattgt tcgttgtctg gaaagagtcg atcggcgcaa
    19501 accttaatcg tcctgcgaaa ttcaacagac cggtccgcgc tgcgagccag ccgcaccatt
    19561 tgaacagctt tccaaggggc gatccggggc tgtaaagccg aaaagacaaa cactggatgc
    19621 gagacgaagc gaggggtacg agaaggcggg gatccgatgg aggcaaaccg ataaaggcgt
    19681 cgggaggtac aatgcaagag gaagcagcag ggttatcctc gtacacgccc tgcttcttcc
    19741 gctctgactt cttcatacag ggcattcaat cgatcgccga cacgatccca aagatagtgt
    19801 tcccgcaccc tcttgatggc tccggctgac agacgctcat acaacgccgg gtcggaggcc
    19861 agccgggaaa gcgcgtccgc cagaccctga atgacggcct ccggtttcgt cagcgccacc
    19921 ttgatcccgc attcttcagt cacatactcg ccagggccgc cgaaatccag gcatacgacc
    19981 ggcagcccct ttgccatcgc ctcaatcacc accatgccgg cgccttcgaa ggaagggaag
    20041 aaaaacacat tactttcctc ctgcatagct tggaagactt tgtcgcgact gatttgacct
    20101 aaaaaaataa cactcttaaa aactccaagt tcagatgcga attttactaa ttcatttttt
    20161 tgaggaccgg taccaataat ttcaagtgta gattttgggt gaacagataa atgtttcgca
    20221 aaggcagcaa gagataaacg gtatcctttt atggaaacca actgaccaac tgatagaatt
    20281 tttaatggat agtgaaaaga tttcgccttt tcagcaagaa tctccttgtc gatagcattt
    20341 tgagaaatag ataggctttt cgtagcatac gtctcttcta acctacttcc cacttcttcg
    20401 ttaatcgtca gtatcagctt agcctttttc ctggcgagag aaatcattgg atcggtgagg
    20461 gggctaattt ttcttatggc atagcgtagt cgattgtctt taatacctga agatccaatt
    20521 aactgataaa attgttttgg tatgtcaggg ttagcgccaa tcggacccca tacaaaaggg
    20581 acaggcaata atgcaatcca tggccccatg tacatattta tgaacgtaat gtggtgggtc
    20641 aaatcgaaat ggacgcgttt cgacagagca aaggcatgtc gataagctat aatttgccac
    20701 aggtagtaat agaggtgaac gccacgtctt ccttttttcc agaaacgcat ccaaggtggc
    20761 agatcgatgt aaacgaaatg caggttggga ttggacttgc ttttgaagtt ttgctcaata
    20821 aggagacggt tattttccct cgtgatcacc catgtttcgt gaaacctggc tatctgctgc
    20881 acccactgcc agccgacctc cggttcggat cctttgccgg gttcgcaggc ataggcggaa
    20941 acaagaacct tcactgtttg ataacaccac cagaagcaaa tggactgcct acccatcgcc
    21001 gtgaaaggaa atcgaagatc acctgcaaca ccctcgtacg cacttcatcc acgcttcccg
    21061 aattggaaat cagcaccgcc cttgattctt gagcagcaaa ggccttcagt gcctcaacct
    21121 ggcgataaat ctcatccatc ggcaattccc gcttgcgggc cataataacc tccggtggcg
    21181 cgtaaagaca aagaatcaaa tctggtttcg gtgcaagagc ggcaaaccat cccgccacgc
    21241 acccggagag tccgatgcgg aaacgacgcg gatcgagggc catgtcgtag gcgtagcggt
    21301 cgaaaataac gattgtgggc tgcgttacga tcatgggacg gatcttcagc cagtagccca
    21361 ggatgtagtc aaaggtgtaa taaacaagtc ttatcagcga tccgacaggt ccggaaggcg
    21421 tggccccatg aggctctagg accggtccct cctggatcgc ggctttacct ttcaaccgcg
    21481 ccaacggggg aagcaggccg gggcgcagat gcagtacatg tgttgcgtta tggttagcct
    21541 tatccaaagc cgatttcatc gtctgaatca cggttgactt ccctgaaccg tcaacgccga
    21601 gtacagctat gataagaccc gtgttgtgca ttttgataaa acaggttgtt acctctgggt
    21661 gtcatgctca taatgccgat ctgaaagtgg gcagtttttt ttgtcttaga aattcattgt
    21721 gtttggcatt ggatactgca ccaacgatac gtaaagctaa ctccttttct cccatgtcac
    21781 tggattggat taacacgaca ggaatcttca gcgattcaag ggtgtttctc aaagaagcaa
    21841 ttctcggtga ggtaaatatt tcataaacga taatggcgat tccgggtttc ggta
//
LOCUS       DAOSIM010000010        21291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_2729_length_21291_cov_7.498399, whole genome shotgun sequence.
ACCESSION   DAOSIM010000010 DAOSIM010000000
VERSION     DAOSIM010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 21291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21291
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2729_length_21291_cov_7.498399"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLR47_00975"
     CDS             complement(<1..255)
                     /locus_tag="PLR47_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOG11534.1"
                     /translation="MTAPNSIEELYRERGSRARELHDAGEKVIGYFCCFVPDEILAAF
                     DLVPYRIQGSQSESIDEADALLEPMACPFARSCFNLALKGR"
     gene            416..1222
                     /locus_tag="PLR47_00980"
     CDS             416..1222
                     /locus_tag="PLR47_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11535.1"
                     /translation="MIQIYEVQKPAEALALIELGVDRIGSVLPDPAKWKSASVRKTVQ
                     AAKAGGAASVLIPIMKDPAAIFAALDYYEPDCVHLCDVLSPWPDERQVAVRDFDALLS
                     LHIDLRDRFPRLDIMRSLSVPRPGLPNASAVEKNILEIAERFAPVTDFFLIDTLLLNA
                     DQPVAGFVGITGETCDWSIAKALIDRSPIPVILAGGLAADNVYDAMTGLRPAGVDSCT
                     RTNATDRSGRPVRFKKDLKKVRRFIEEVRRADLLLEGVPRGAKSIDRGRI"
     gene            1188..1523
                     /locus_tag="PLR47_00985"
     CDS             1188..1523
                     /locus_tag="PLR47_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HOG11536.1"
                     /translation="MGQNPSIADEYKTITAMISLFCRERHQTGGKELCPSCAELLAYA
                     KTRLDKCPFAADKPTCAACPVHCYRPGRRRQIQEVMRCAGPRMLRSHPILAIRHLLKK
                     FKKPGALKP"
     gene            1520..2635
                     /locus_tag="PLR47_00990"
     CDS             1520..2635
                     /locus_tag="PLR47_00990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG11537.1"
                     /translation="MKIAVIGAGPGGLYAALAAARKNITVDLFEKRKVGEGIVCGECI
                     FDSLGIMTRPGRGLLHPVKGLVLKAGGEYRFPLGSHKPLWMLDRRIWQQDLSRQAKTL
                     GVRLHEQAGVTVSDLVRMKKSYDWILDASGAPSVASRLCGFAREYVQECLAAHQVRLA
                     GDFSSLVPCIRVVFFPDLPVENQPGYAWVFPRDERSANVGVVCTVRKTQGPGRPDMKK
                     MLADFLRREGLAGASVLARGGGIAPARMLSRLVYDNVLLVGDAAGLTSALHGGGIDMA
                     CLSGVLAVKALSGGQAGVARYAGWLERYMREKNALEKVTIGKMRSLTFGEFDRLLSGV
                     TSASLRVRFWTALQNPLMFWTTIRWFKTKKKIPDWPV"
     gene            2830..3960
                     /locus_tag="PLR47_00995"
     CDS             2830..3960
                     /locus_tag="PLR47_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11538.1"
                     /translation="MKKQSPVKNHRVSLTLPAIIFAILFIFAAASCTHLPRPVENIGQ
                     SSEFLDGGKNQPGAKDRARHTLTGKRPFEWWYFDGHLDTGETFVGVFFDPNFTNGKPG
                     ATFSLYSPDWKKMSVLQSLQAEEMKSSTEDVQIACPLGFVRRMDDKQYHVRWNMEGVT
                     ADFKLTMTAPGWMLDGQDESRDFFWSIHQARNRIEGAITQNGQTRQVKGEGYADHNWG
                     RKPLNEITRDWVWGRIFSDEYTIVYADVDYIDPAMNRARPLYIARGDKIIVGAASPTV
                     RQRDFATHPVLKRHYPKQVLIDFEEGGVGAHLNIRFKALVEEVDLLTVAGLNPFSQWI
                     ARTFIARPVYFRIIADYQGDIIEHGKATPIAGECLYEIMGFE"
     gene            4104..6629
                     /locus_tag="PLR47_01000"
     CDS             4104..6629
                     /locus_tag="PLR47_01000"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOG11539.1"
                     /translation="MKICCIAAVCLLSASCATLMDRHFKDKLDATQGTAVLTGLRDTA
                     TVSRDAYGIPFVKANNMEDLAMTVGYVHASDRLAQMIGLKLIAEGRLAEMAGPALLNL
                     DIYMRTMNLKRAAESLYGNVSAENRRLLERYSDGVNAYLDQHKDKLPPGLALSGYKPE
                     PWKPMDSITICVLLSFGLSFNLPEEIAALNIAQAVGAEKTAWLLPIYPDEPIPLGEAA
                     KLRGVDLKKAAESVAGIDELQPFLSSLGLSGAAASNNWAVRGEKTKNGASIFCSDMHL
                     PLSMPSMWNMIHLRCGAYDVAGMSVAGAPMIVAGYNGHIAWGATMVMADNQDLFLEQL
                     RNVNGKLHFLYKGEWLPVVEHQEIFNIKGKPPVARTFRETVHGPLLNEALQAEPIHVI
                     LPASVDLPYGVALSRTVPAADDNSMNAFFQLSFAHSVEDAIPLIKQIRAIPLNMVFAD
                     KDHIAWQVIGNYPVRAKGRGLMPSPGWTGVYDWTGFLDADGLPNAQNPPAGFIGTANH
                     RTVPKDYPHILSSSWYWPERAERIVQMASATDQHTTQTNREMQLDTYSLFVPKLKDAV
                     LKGDLAKEIAREISRWKDEKKAAGARLALAMLRNFSGDMRADSKEAALAGAFLQSATR
                     DIFQDELGPDDSRAWKSFLIADNESYNATCDHLLVRGDESPFWDDIHTPEKETKAQII
                     ALSLADAVAFLESTLGDDTGRWTWGALHTSTWETDTSQMAPHFGFIERTALRSLWSYF
                     NRGPYPAGGDMFTLNVSMYMMGRNFNTWLIPSMRMIVDFSKEEPMLAVNSSGQSDNPS
                     SPHYDDGIGAWRDGKYIPFPFRDAAVRDQYREVMQFRPSAIVR"
     gene            6679..7680
                     /locus_tag="PLR47_01005"
     CDS             6679..7680
                     /locus_tag="PLR47_01005"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HOG11540.1"
                     /translation="MRKILTFLLIAILLSGCAAGKSAHIPLEDAGHKDAAYEWAPHDD
                     GGEWWYLTGYAPDQNNKLYFYQFTIFHGYAARLLEAYALHLTFTDCSTGQHVFSESIQ
                     FSSDEVYADSQCVVFQDSRICLDDRRIKIVGASENLKMEIEGTSTKKAAWHGKDGVIV
                     MGHPEEPKERSYYYSFTNIATRGVIEFKDEKGRWVKIDGNGKAWFDRQWGRFSELGWE
                     WFSFRFFDDEEIMLFPFPKTGVKEGTYIDKQGNVAAFDNYAYSVKKFKTFNGKKIGLG
                     WNVTVPFKEKEYEVIPIVEDQYNPSKLQEYWEGICRLQNKKGELVGYTIIETTGSAY"
     gene            complement(7718..9766)
                     /locus_tag="PLR47_01010"
     CDS             complement(7718..9766)
                     /locus_tag="PLR47_01010"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOG11541.1"
                     /translation="MNPFGIALILLISALLSGSLAVYAWRRRGKPGAGIFFFLMLGTT
                     IAVFAYGMELLSATLSGKLAWVRIRYAGTALIYPMYLLLALWYTNKKKWFAPGRVALL
                     FVLPVFSLIVLFTNSLHHLHYASISLNTSGPFPMIAKTYGPLFAFYFITSFGYVLLAL
                     AILGSNYLVTSPFFRRQTAALMLGTIAPLAAAWLYNTGMVKPLGGLNILPFAYIFSSL
                     TAAWVILGRKLFDLRPFAHGMIFENMTDGFIVLNKQLEILDANPPALRLLDLLPDHMG
                     QFGNKAFVNHPALIRLIHNEPHAHVQIEQDGQVFDAFRTSLFDQQQNLAGHLISLRDV
                     TSIKKAEEALRKSENQYRFLAENMNDVLWMMDLDLKTVYVSPSIEAVLGFTQEERLRQ
                     DVQQQLTPQSLRIARETLAREKVLEKQTGDPKRKATLTLEFCHRDGSTRWLETIISGI
                     RDDQGTLTGLYGASRDVSGRKEAEASILRFSEELREKNKELQCLYSVSEMVRKGQTLQ
                     EDILSACVSLIPQAYDCPEIAGCRIPWEDREYKTENFARTSWMQTSDIRVRGNSIGKI
                     EVCYLEPRPEKDEGPFSKEDRKFLDGIADLLGKSYERRLAAEEREKLIEDLKNALQEV
                     KTLTGMLPICASCKKIRNDQGYWERIERYISERSSVQFSHGICPECMKKLYPEMADHS
                     "
     gene            10097..10399
                     /locus_tag="PLR47_01015"
     CDS             10097..10399
                     /locus_tag="PLR47_01015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HOG11542.1"
                     /translation="MITVIAKLPVKEGKMDEAMAAFQVLISKVASEEEGTVLYSLNRD
                     KANPDVLVVVEQYRDKAALEFHSSTPHFKEFFEASTAFIGGRPEMSVMKEIQRAKP"
     gene            complement(10678..11469)
                     /locus_tag="PLR47_01020"
     CDS             complement(10678..11469)
                     /locus_tag="PLR47_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG11543.1"
                     /translation="MFLTRFFRRVINFCLTLGLWVYFMLGYLVLMFVLFIPCYSRLKA
                     VDAVLQKSFHLHLKVFFALVNRLSPGVRFAVSEDVRNLRSSIIVCNHLSYLDPILLVS
                     LFPRQTTIVKNTFFSVPIFGWFLRKAGYVPSSPTEMYGPAMINHLEDIKRQLAAGGNL
                     FVFPEGTRGRRGRIAPFNRGVFSIARYCSTGVKMVLIRNTERLFRPGSFSFDTRGKFT
                     VSMELIGSLTPDQLKNASISASADQARSLFERKIREAKTGGDGRA"
     gene            complement(11475..12716)
                     /locus_tag="PLR47_01025"
     CDS             complement(11475..12716)
                     /locus_tag="PLR47_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOG11544.1"
                     /translation="MPQRRVVITAASAISPIGHGRKPIVESLVNGKSGVAALREDHLI
                     SRFIHSRVYGTVGYPIAYDFSRQYRKTMGPVSYYACQVAREVLEQAGLPPDFVTGGRL
                     GIAFGSIHGSPTVQRDIYRIFFEAKDKATFQGIGAVDYLKSMVHTTAVNISKMFGITG
                     RIISSGTACTTASQSIGFGYEAVKYGLQDAMICGGADEYDTITVAVFDNLLACSTAFN
                     DRPGETPRPFDRARDGLVVAEGAGAVMLEEYEFARKRGATILAEVIGFACNSNGGDLI
                     LPNLDGITKTLRLGLENAQINASDVDLVSAHATATKMGDIIEAQATGRVFGTQPRVVA
                     LKSYMGHTMAACGAVETVLTIYMMQEGFIAPTLHLEEIDERCAMIRHVQKLEEIPVKT
                     AAIQNFAFGGVNTCLMIRKLT"
     gene            complement(12717..12989)
                     /locus_tag="PLR47_01030"
     CDS             complement(12717..12989)
                     /locus_tag="PLR47_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOG11545.1"
                     /translation="MTRQEIEKEIRAVLLREFEVENPEPDVNLREAYGFDSIDAIELL
                     LEIERFLGTELTQAEKKKAMDIRTLNQIIDYVEMLAHHRKNEAEKA"
     gene            complement(13052..14524)
                     /locus_tag="PLR47_01035"
     CDS             complement(13052..14524)
                     /locus_tag="PLR47_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG11546.1"
                     /translation="MRKPAEGKNTVIIGSGVGGLSAGILLSLLGFRVTVVEKNAEPGG
                     LMRSYSRRGIDCPVGVHYVGALGPSEPLGKMFRVLGIDPDSLFSRMKNRAVIDRYLFD
                     DFVFDLPEGLDAYEKNLREACRADGAALDALMKGLRGTAGVMAASPFFFEGGSPFANM
                     DSFRPMGELLCELGASSKLRAVLAAPCQLVGVELKDCPVIIHQMVLAGYLFSSWRPKS
                     GRDLADAFAARFSQLGGRLRLSSAVRHIRTKQGRVAGVDLEEGDSLPADSVVAAVHPK
                     VLIGLLDPEALRESQRERIAGLEETGGVLAVQAGVDAEAHPAVDYNLYRLRAGGSGEI
                     ENGFFCQVLKGDGRNTNLLSIITGSHYRDWLPWEDTLTRRRGEAYEQKKQALAEALVA
                     ESSAVFGPLKNLQLLDVFTPLSLRDHVSCPEGSCYGVLRSSRHLLKIASLGNLPIGGL
                     YLAGQNAMAPGVMGCILGSLGVARKMAGNERFGKELERLL"
     gene            complement(14543..15205)
                     /locus_tag="PLR47_01040"
     CDS             complement(14543..15205)
                     /locus_tag="PLR47_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11547.1"
                     /translation="MNLRLLLILPLLLASCSSLPEIRPSAVVAGKVIACPSPFVARKT
                     LFIHAVEAAAAGETRAVMIGVTVADPASRTLSCALMSPEGMVLFEASRNPSGTQISRA
                     LPPFDEEEFARGLLDDIELIFFEPPEPVDRAGFSDTGRFVCRRRLQGGGWLDVGKDGD
                     GRVQIRRYSKGGTLRRSVALAAGANPYAAIELQASEWIGYSLSMTLVEPEAVLDEALS
                     GK"
     gene            complement(15202..15828)
                     /locus_tag="PLR47_01045"
     CDS             complement(15202..15828)
                     /locus_tag="PLR47_01045"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOG11548.1"
                     /translation="MRGQAGRRLLILLFLLLTAFPDAAGADDFDRLRRDASGISSLSA
                     DFVQKKSMKILSAPLVSKGKFYYAAPDSIRWEYVKPIRSIVISNQGSTKRYIASGGKM
                     IEDKTGGALAMKIVLDEVAGWMKGKFTANPSFAARVQEGPETQIVLTPTGKNTAGLIE
                     KIEITVSRKDAVVKTVRIVEGAAAETRIDFLHTVVNQTLPPGTFQDIP"
     gene            complement(15828..16562)
                     /gene="fabG"
                     /locus_tag="PLR47_01050"
     CDS             complement(15828..16562)
                     /gene="fabG"
                     /locus_tag="PLR47_01050"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOG11549.1"
                     /translation="MEERKIALVTGASRGIGRATAVELARTGCHVIINYNASEAAAAE
                     TLAQVQAAGGSGEMRKFDVSDPAAVKEAMAAILQEHKRIDVLVNNAGVTADGLFMMMG
                     EEEWHRVIQTTLNGFFHVTKPVLREMVRKKRGSIVSVSSISSMVPNRGQANYAAAKAG
                     INAATSSLAKEVARFGIRVNAVAPGPIATDMLKSMPMDESIIKSVIPMARVGKPEEVA
                     RVIRFLCSEDASYITGQVISVNGGMF"
     gene            complement(16596..17012)
                     /locus_tag="PLR47_01055"
     CDS             complement(16596..17012)
                     /locus_tag="PLR47_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11550.1"
                     /translation="MIDIESVIPHREPIKIITEAIELQDDAGTAAAVVNERWPLFDGR
                     TVHSLVLVEAVAQTAALVEGYKRKKRGQDSIKGWLVGIKSAEFHRELLALQTRITVSI
                     KSLYSFDDYAVINGIVTAENETLLTAVLQVVRLNQD"
     gene            17168..18550
                     /locus_tag="PLR47_01060"
     CDS             17168..18550
                     /locus_tag="PLR47_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HOG11551.1"
                     /translation="MSAELKDPFMTACRKLLSVPKALCRKEFTCSGDTYGEVFSLAAG
                     LKKTLSGRGAEKSVCLCTENKAVFAASLLAALAGGCNLILPYSFSTHALLEMYEATGF
                     DAAIADRPEEMPGVEMITPTAGDPADLNPGGLIDPDRSFLKLFTGGSTGQPRVWSKTP
                     RNLFAEAFYLRDHFGLTGDDVFLATVPPYHIYGLLFSILLPLVARARVLPEIYTFPQE
                     ILSTVNRHRATVLVSVPISYRSLRVDNLSVPSLRLAFSSSGPLDRSDARYFLKKTGLG
                     IHEIYGSTETGGVASRSVSENTESWQPAGPVSWRMFGRRLAVRSDFVSSEMERDAEGF
                     CVTGDEVQPDRDGRFLLLGRADGIVKVAGKRVDLQEVQNKIQSLPTVRDAVVIALPAE
                     KGRESEIAALVACDLTEIQIKKMILEKLEPYAMPRRFHIVSSIRRTATGKIDYRRVEQ
                     IFLREKGKSQ"
     gene            18864..20186
                     /locus_tag="PLR47_01065"
     CDS             18864..20186
                     /locus_tag="PLR47_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOG11552.1"
                     /translation="MARFREYENYDGLGLAELVRKKEVTPPELCEEAVLRIDQLNPKL
                     NAVIYRMDDAARAAAAGPIPEGPFSGVPFLCKDLVSAIAGVPMTKGSKSCRNYVPSYD
                     SEMARRFRAAGLIVLGKTNTPEFGLIGLKPTRGRNPLGPESGEVWQGAEVEGALTRSV
                     RDAAAMLDATNGADSGAPYILPRPARPYLEETRQDPSRLKIGFTVESPLKNAVHPECV
                     EAVKNAAKLLERLGHKVEEARPDIDGEALGRSYMMLYFGEVAADIRELASVVGRPLTH
                     SDTEEATWALNLLGKTFSAGDFVLAKRQWNTFARQMAKFHETYDLYLTPTIASLPPRI
                     GEMQQKPAELAFLKIINGLNLGGLLKATGIADMLATKGLSKMPFTQLANLTGQPAMSV
                     PLHWSREGLPCGVQFVGRFGEESLLFQLAGQLERAQPWFDRRPALTKS"
     gene            20523..>21291
                     /locus_tag="PLR47_01070"
     CDS             20523..>21291
                     /locus_tag="PLR47_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG11553.1"
                     /translation="MSGFVNKTMGDVIEETAGRFPDHPALIAPQFQVRLNYRELYEQC
                     RKTARGLMAMGVRRGDHVSVWTTNVPEWVYLQFALGMVGGILVTINTNYQSHELEYIL
                     RQSDSTTLFLIDQYRDTSFYETVRKVVPELGSCEPGKTASAKLPVLKNVVYIGNRESA
                     PGMFKFSDLIAMGESVSEQELGERMSSLTDDDVINMQYTSGTTGFPKGVMLTHHNVIN
                     NARMVGDVMGLTDKDTLLIQVPVFHCFGCVMSTLNSVY"
BASE COUNT         4526 a         6046 c         6278 g         4441 t
ORIGIN      
        1 ccggcccttg agcgccaggt tgaagcagct gcgggcaaac gggcaggcca tcggctccag
       61 gagcgcgtcc gcctcgtcga tggattcgct ttgcgagccc tggatgcggt agggcaccag
      121 gtcgaaggcc gcgaggattt catccggcac aaagcagcag aagtagccga tgactttttc
      181 ccccgcgtcg tgcagttcgc gcgcgcggga gcctctttcc ctgtagagtt cctcgatgga
      241 gttgggtgcc gtcatgcaaa accccctgta ttcgtttttg aatgggcccg attattcagt
      301 gaaacgcgga taaaagcaag ttgctttttg accttgcccg gcgggcggaa atcactccgt
      361 cgcagatctt gattgggcgc ggcggtcatg ataagtcatt accatgcgga aaacgatgat
      421 tcagatttat gaagtgcaaa agcccgcgga agccctcgca ttgatcgaac tcggcgtgga
      481 ccggatcggc agcgtgcttc ccgatccggc aaaatggaaa agcgcgtccg tccgcaaaac
      541 cgtgcaggcg gcgaaggcag gcggcgcggc cagcgttctg attccgatca tgaaagaccc
      601 ggcggccatc ttcgcggcgc tggactacta tgagccggat tgcgttcacc tgtgcgatgt
      661 cctttcgccc tggcccgacg agaggcaggt cgcggtgcgg gacttcgacg cgcttttgag
      721 cctgcacatc gaccttcggg accggtttcc gcgcctcgac atcatgcggt ctctgtccgt
      781 tccccggccc gggttgccca acgcctccgc cgtcgaaaaa aatattctgg agattgcgga
      841 gcgcttcgcg ccggtgacgg atttctttct gatcgacacg ctgcttttga acgcggacca
      901 gccggtggcg ggctttgtcg gcatcacggg ggaaacctgt gactggtcta ttgccaaagc
      961 cttaatcgac cgaagcccca ttccggtgat tctggccgga ggactggccg ccgacaatgt
     1021 ttacgacgcg atgacgggcc tccggccggc cggtgtggac agctgcacga ggaccaacgc
     1081 gaccgaccgg agcggaaggc ccgtgcgctt taaaaaagac ctgaagaaag ttcggcggtt
     1141 catcgaagaa gttcgccgcg ccgaccttct tcttgaagga gttccccgtg gggcaaaatc
     1201 catcgatcgc ggacgaatat aaaaccatca cggcgatgat ttccctgttc tgccgggagc
     1261 ggcatcagac cggcgggaaa gagttgtgcc cgtcgtgcgc cgagctcctc gcctacgcga
     1321 aaacgcgcct ggacaagtgc ccgtttgccg cggataagcc gacctgcgcg gcctgcccgg
     1381 tgcattgcta caggcccggg cgcagacggc agattcagga ggtgatgcgc tgcgccggtc
     1441 cgcgcatgct gcgctcccat ccgattctgg cgattcgcca ccttctgaaa aaattcaaaa
     1501 agcccggagc gctaaagcca tgaagattgc cgtcatcggc gccggacccg gcggccttta
     1561 cgccgcgctg gcggcggccc gcaaaaacat taccgtggac ctgtttgaga agaggaaggt
     1621 cggggaaggc atcgtctgcg gcgaatgcat cttcgattcc ctgggaatta tgacccggcc
     1681 gggccgggga ctgctgcatc ccgtaaaagg gcttgttttg aaagccggcg gcgagtaccg
     1741 ctttcccctg gggagtcaca agcccctgtg gatgctcgat cgccggatct ggcagcagga
     1801 tctttcccgt caggccaaga cgcttggcgt tcgcctgcac gaacaggcgg gcgtgacggt
     1861 gtcggacctg gttcggatga agaaatcgta tgactggatt ctggacgcca gcggcgctcc
     1921 gtccgtggcg tcccggcttt gcgggtttgc gcgcgagtat gtccaggagt gcctcgcggc
     1981 gcatcaggtg aggctggccg gcgatttcag ctcgcttgtg ccctgcattc gggtcgtctt
     2041 tttcccggat cttccggtcg aaaatcagcc cggctacgcc tgggtttttc ccagggatga
     2101 gagaagcgcc aacgtcggcg tggtctgcac ggtgcgcaaa acgcagggac ccggacggcc
     2161 ggatatgaag aaaatgctgg ccgacttctt gcgccgggaa ggacttgccg gcgcatccgt
     2221 tctggcaaga ggcggcggca tcgctcctgc ccgaatgctt tcccgtctcg tttacgacaa
     2281 cgttcttctg gtgggcgacg cggcggggct tacctccgcg ctgcacggcg gcggcatcga
     2341 catggcctgc ttgagcggag tgctggccgt gaaggcttta agcggggggc aggccggcgt
     2401 ggcccgctat gcgggatggc tcgaacgcta catgcgcgag aaaaatgccc tggagaaagt
     2461 caccatcggc aaaatgcgct ccctgacctt cggcgagttc gaccgtctgc tctccggcgt
     2521 gacctccgcg agcctgcggg tccgcttttg gaccgctttg caaaatccgc tgatgttctg
     2581 gacgacaatc cggtggttca aaacgaagaa gaaaataccc gactggccgg tttgaacacc
     2641 cgttttgccc cgtatggctt gcggaagcaa aagcagcctg tccagggatc aggaacgctt
     2701 ttcctgccgc ggtgttttga ttttcaaaag tcatgcggca tttcaattgg tcatgcgcag
     2761 cattcgcgca ggatgcatgg cgagcaagat tttcttgaca gccgggttcg aaattcttat
     2821 aggacgcata tgaaaaaaca atcccctgtc aaaaaccacc gcgtatcgtt gacgcttccg
     2881 gcaatcattt tcgccatcct gttcatcttt gccgccgctt cctgcaccca tctgcccagg
     2941 cccgtcgaga acatcggcca aagcagtgag tttctggacg ggggcaagaa ccagcccggc
     3001 gcaaaggaca gggcgcgcca taccctgacc ggcaagcgtc cctttgagtg gtggtacttt
     3061 gacgggcatt tggataccgg tgaaaccttt gtcggggtat tcttcgaccc gaactttacc
     3121 aacggaaaac ccggggccac cttttcgctc tacagcccgg actggaagaa aatgtccgtt
     3181 ctgcaatccc tgcaggcgga agagatgaaa tcctcgacgg aggatgtgca gattgcctgc
     3241 cccctggggt ttgttcgaag aatggacgat aagcaatacc atgtccggtg gaacatggaa
     3301 ggcgtgacgg cggatttcaa gctgaccatg acggcgccgg gatggatgct cgacggtcag
     3361 gacgaatccc gggacttttt ctggagcatc catcaggccc gcaaccgcat cgagggcgcc
     3421 atcacgcaaa acgggcaaac caggcaggta aaaggagagg gatacgccga ccacaactgg
     3481 ggaagaaaac ccctcaacga gatcacacgg gactgggtat ggggaaggat cttctcggac
     3541 gagtacacca ttgtttatgc ggacgttgat tatatcgatc ccgccatgaa cagggcaagg
     3601 ccgctgtata tcgccagggg ggacaagatc atcgtgggcg ctgccagccc tacggtccgg
     3661 cagcgggatt tcgccaccca ccccgttctg aagcgccatt atccgaagca ggtcctgatc
     3721 gactttgagg agggaggcgt tggggcgcat ctgaatatcc ggttcaaggc tctggtggaa
     3781 gaggttgatc ttctcaccgt tgcgggattg aatccctttt ctcagtggat cgccagaacg
     3841 tttatcgcaa ggcccgtcta ttttcggatt attgccgatt accagggcga catcatcgag
     3901 cacggcaagg caacccccat agcaggtgaa tgcctctatg agatcatggg cttcgagtag
     3961 cgctccgccg tgaacggggc tgatctctcc gccggattca tcaacggatc gcaaaaacat
     4021 tttggaaaat gatttgagga ggtccgatgc agaaccctac cgcacatcct ggaatgagac
     4081 ggggtcatcc ggggaagcaa ttcttgaaaa tctgctgtat tgccgccgtc tgtcttctct
     4141 ctgcaagctg cgccacgctg atggaccggc atttcaaaga caaactggac gccactcagg
     4201 gaacggcggt cctgacgggg ctcagggata ccgcaacggt aagccgcgac gcttacggca
     4261 ttcccttcgt gaaagcaaac aacatggaag acttggccat gaccgtaggc tatgtgcatg
     4321 cctcggaccg gctcgcccaa atgatcggct tgaaactgat agcggagggc cggctggccg
     4381 agatggccgg accagccttg ctgaatctgg atatttacat gcgcaccatg aatctcaaaa
     4441 gagcggcgga gagtctttac ggaaatgttt ccgccgagaa ccgccgcctt ttggagcgat
     4501 acagcgacgg cgtaaatgct tatctggatc agcacaaaga caaactgccg ccgggtcttg
     4561 cgctgtcagg gtataagcct gaaccctgga agcccatgga ttccataacg atatgcgttt
     4621 tgctgagctt cggtctttcc tttaatcttc cggaggaaat tgccgccttg aacatcgctc
     4681 aagccgtggg cgccgagaaa acagcctggc tgctgcccat ctatcccgat gagcccattc
     4741 cactcgggga ggccgccaag ctcaggggcg ttgacttgaa gaaggccgcc gaaagtgtgg
     4801 cgggaataga tgaactgcag ccgtttttga gctccctggg cttgagcggc gccgccgctt
     4861 ccaataattg ggccgtccgc ggggaaaaaa caaaaaacgg cgcgagcatt ttctgcagcg
     4921 atatgcatct tcctttaagc atgccgtcca tgtggaacat gatccatctc cggtgcggcg
     4981 cctatgacgt ggccggaatg agtgttgcgg gcgccccgat gatcgttgcc ggatacaacg
     5041 gccatattgc gtggggcgcg accatggtta tggcggataa ccaggacttg ttcctggagc
     5101 agctcaggaa cgtgaacggg aaactgcatt ttttatacaa aggagagtgg ctgccggttg
     5161 tcgaacatca ggaaatattc aacatcaaag gcaagccgcc cgttgcccgg acttttcgcg
     5221 aaaccgtgca cgggccgctt ttaaacgaag cgctgcaggc ggagccgatt catgtcattt
     5281 tgccggcctc cgtcgatctg ccttacggcg tggcgttgag ccgaacggtt cccgccgcag
     5341 atgacaacag catgaatgct ttctttcaac tcagtttcgc ccactcggtg gaggatgcga
     5401 ttccgctgat aaaacagatc agagccatac cgctgaacat ggtatttgcg gacaaggatc
     5461 acatcgcctg gcaggtgatc ggcaattacc ccgtccgggc caaaggccgc gggctgatgc
     5521 cttcgccggg ctggaccggc gtttacgact ggaccggctt tttggatgcc gacggattgc
     5581 cgaatgccca aaaccctccg gccggattta tcggaaccgc caaccaccgc acggtgccga
     5641 aggattatcc ccatatcctt tcctcttcct ggtactggcc cgaaagggcc gagcgcatcg
     5701 tccagatggc gtcggcgacg gatcagcaca ccacccaaac caaccgggag atgcagttgg
     5761 acacctactc cctgtttgtg ccgaagctca aggacgctgt gttgaaagga gatctggcca
     5821 aagagattgc aagggaaatc agccggtgga aagatgaaaa aaaggcggca ggcgcgcgtc
     5881 ttgcccttgc catgctgcgg aacttcagcg gcgatatgag ggcggattcc aaagaggctg
     5941 ccctggcggg agcgttcctg caaagcgcca ccagagacat ttttcaagac gagctcggcc
     6001 cggatgattc cagggcgtgg aagtcgtttc tgatcgccga taatgagagc tacaacgcca
     6061 cctgcgatca tctcctggtc cggggcgatg aaagcccctt ctgggacgat atccatacgc
     6121 cagaaaaaga gaccaaagcg caaatcatcg ccctttcgct cgcggatgcc gtcgcatttc
     6181 tggaatcaac tttgggagat gacaccggcc ggtggacatg gggagcgctg cataccagca
     6241 cctgggaaac cgatacttct caaatggcgc cccattttgg ctttattgaa cggacggcgc
     6301 tgaggagttt gtggtcatac ttcaaccggg gtccgtatcc cgccggggga gacatgttca
     6361 cgctgaatgt atccatgtac atgatgggca gaaatttcaa tacctggctg attccttcca
     6421 tgcgcatgat cgtggatttc agtaaggaag agcctatgct ggcggtgaac agctccggcc
     6481 agtcggacaa tccttccagc cctcactatg atgacggcat cggggcctgg cgggatggaa
     6541 agtatattcc attccccttc cgggacgccg ccgtcaggga tcaatatcga gaggtgatgc
     6601 aattcaggcc atccgcgatc gtgcggtagc ttcccctgca aaacgaagcg gcaagactca
     6661 atgcaaaagg aggatcctgt gagaaaaata ctgacattcc tgttgatcgc gattttgctg
     6721 tcgggttgcg ctgcgggcaa atcagcccat attccattgg aggatgcggg tcacaaagac
     6781 gcggcttatg aatgggcccc ccatgacgac ggaggagagt ggtggtatct caccgggtat
     6841 gcgccggatc agaacaacaa attgtacttt tatcaattta caatattcca cggatatgcg
     6901 gcacgtctcc tggaagcgta tgcgctccat ttgacgttca cggattgctc aaccggacag
     6961 catgtttttt cagaatccat tcaattcagc tctgatgagg tctatgcgga ttcacaatgc
     7021 gttgtctttc aggacagccg gatctgcctg gatgaccggc ggatcaaaat cgtgggcgca
     7081 agcgaaaatc tcaaaatgga gattgaaggc acgtccacga agaaggctgc gtggcatggg
     7141 aaagacggcg tcatcgtcat ggggcatccc gaagaaccaa aagagagaag ctactactat
     7201 tcctttacca atattgccac aaggggagtg atcgaattca aggatgaaaa gggccgttgg
     7261 gtcaagatcg acgggaacgg caaggcgtgg ttcgatcggc agtgggggag gttcagcgag
     7321 ttgggctggg aatggttttc cttccgattc tttgatgacg aggagatcat gctctttcct
     7381 tttcccaaaa caggcgtcaa agaaggaacc tacatagaca aacaaggaaa cgttgccgcc
     7441 tttgacaatt atgcctacag cgtaaagaag tttaaaacgt tcaacggcaa aaagatcgga
     7501 ctgggatgga acgtgaccgt tccctttaag gaaaaggaat acgaggtaat tcccattgtt
     7561 gaggaccagt ataatccgtc aaagctgcag gaatactggg aagggatctg cagactgcaa
     7621 aacaaaaaag gcgagcttgt cgggtacacg attattgaaa ccaccgggag cgcctactga
     7681 cgggcaggca ggatcggtcc ggaagcattc cccgcgctca ggaatggtcc gccatttccg
     7741 gatacagttt tttcatgcac tccgggcaga ttccgtggct gaactggacc gatgaacgct
     7801 cggagatgta gcgctcgatt ctttcccagt atccctgatc gtttcgtatt tttttgcacg
     7861 acgcgcagat cggaagcatg ccggtcaggg tcttgacctc ctgcagggca tttttaagat
     7921 cctcgatgag cttttcccgc tcttcggcgg ccagccgccg ttcgtatgat tttcccagca
     7981 ggtcggcgat gccgtcgagg aacttgcgat cttcctttga aaaagggcct tcgtcttttt
     8041 ccggccgcgg ctcaaggtag cagacttcga tctttccaat cgaattgccg cgcacacgga
     8101 tgtcgctcgt ttgcatccag gaggtccggg cgaagttttc cgtcttgtat tcccggtctt
     8161 cccacggaat ccggcagccg gcgatctccg ggcaatcata ggcctgcggg atgagggaga
     8221 cgcaagctga aagaatgtct tcctgcaagg tttgtccttt tcgaaccatc tccgacacgg
     8281 aatagagaca ctgaagctcc ttgttttttt ctctgagctc ctccgaaaac cggaggatgc
     8341 tggcctccgc ttccttgcgc ccggaaacgt cccgggatgc gccgtacagg ccggtcaacg
     8401 tgccctgatc atcacggatg ccgctgatga tcgtctccag ccagcgggtg gagccgtccc
     8461 ggtgacagaa ttccagcgtc agggttgctt ttcgtttggg atcgcccgtc tgtttttcga
     8521 gcactttttc ccgggccagc gtctcccgtg caatcctgag agattgcggc gtcaactgct
     8581 gctgaacgtc ctgacgaagg cgttcctcct gcgtgaagcc gaggacggcc tcgatagagg
     8641 gagaaacata gacggttttg agatccagat ccatcatcca caggacgtcg ttcatgtttt
     8701 cggccagaaa ccggtactgg ttttcgcttt ttctgagggc ctcctccgcc ttcttgatgg
     8761 aggtgacatc gcgcaggctg atcaaatggc cggccagatt ctgctgctgg tcgaagaggg
     8821 aagttcggaa tgcgtcgaag acctgtccgt cctgttcaat ctgcacgtgg gcgtgaggtt
     8881 cgttatgaat cagccggatc agcgccgggt gattcacaaa cgccttattc ccgaattgac
     8941 ccatgtgatc cggtaaaagg tccagcaggc gcaatgcggg gggattggcg tccagaattt
     9001 cgagctgctt gttcaggaca ataaatccat ccgtcatgtt ttcaaaaatc attccgtggg
     9061 cgaaggggcg cagatcgaaa agcttgcggc ccaggatgac ccatgcggcc gtcaggctgg
     9121 aaaagatata ggcaaaagga aggatattca atccgccaag gggtttcacc atgccggtgt
     9181 tatacagcca ggcggccgcc aggggggcaa tggttcccag catcagcgcc gccgtttgcc
     9241 gccgaaaaaa aggcgacgtg accaaatagt tcgatcccag gatggccagc gccagcaaaa
     9301 cataaccaaa ggaggtgatg aagtaaaacg caaacagggg cccgtatgtc ttggcgatca
     9361 tggggaaggg gccgcttgta ttcaggctga tcgaggcata gtgcagatgg tggagtgaat
     9421 tggtgaaaag cacgatcagg gagaagaccg gcaggacaaa cagcagggcg acacggccgg
     9481 gagcaaacca ttttttcttg ttggtgtacc aaagcgcgag cagcaggtac atcggataga
     9541 tcagggcggt tcccgcataa cggattctca cccaggccag tttgcccgaa agggtggcgg
     9601 aaaggagttc catcccgtag gcaaagaccg cgatcgtcgt ccccagcatt aaaaaaaaga
     9661 aaatgccggc tcccggtttg ccccgtcggc gccaggcata gaccgcgagg ctgccggaga
     9721 ggagggcgga aatcaataaa atcaatgcga ttccaaaggg attcatgaaa atgaatttcc
     9781 ttaagtcatt tcagggcgac ggattttgct gcccgcgcat cgatcaaccc ctgcaacaat
     9841 ttcaagtcgt ttgggcaaat gcaatcatga gttgatggta tccattgcat attttatctg
     9901 ttttttcaat tctttcgagc agaaaatcat ttttttaacc atgccgtccg gttcgcggaa
     9961 accagccggc tgcaggggaa cggcctcacc gccgccgttt tggccttccg aagggcttgc
    10021 cactttgagc gtgtttgtgc tatgggggcg aatcgagtcg aaaatgcttc aaaaaaacca
    10081 ttcgggaggt gttacgatga ttaccgttat tgccaagctg cctgtgaaag aggggaagat
    10141 ggatgaagcc atggccgcct tccaggtgct gatctccaaa gtggcttccg aggaagaggg
    10201 aacggttctt tattcgctca accgcgacaa agccaatccc gacgtgctgg ttgtggtcga
    10261 acagtacagg gacaaagccg cactggagtt tcattcctcc acgccgcact tcaaggaatt
    10321 tttcgaggcc agcaccgctt ttatcggcgg ccgcccggaa atgtcggtga tgaaggaaat
    10381 ccagcgggcg aagccgtagg aaacggccgc catccgcagg caggccgcga ccatttgcca
    10441 ttgattggaa aatagaatca tagcggagtc aaaggcgcac ctgaaggtgc gccctgcatt
    10501 aaaacgtagg gcgggtcttt agacccgccg aaaccgggat gcaagttgtc gagcccgctc
    10561 tgattttcag acgttcatgc aattcgatcg ccggtgcggg ccgcggccgc gatccattcc
    10621 tccgccattc ggtgggtgat tggcttcatg cttcctccct gatgcccccg caggggctta
    10681 tgcccgcccg tccccgcctg tttttgcttc tctgattttc cgctcaaaaa gcgatcgggc
    10741 ctgatcggcg gacgcgctga tcgaagcatt tttcagttga tccggcgtca gcgatccgat
    10801 cagctccatc gaaacggtga attttccccg tgtgtcaaag gagaacgatc cggggcgaaa
    10861 cagccgctcg gtgttgcgga tcagcaccat ttttacaccc gtgctgcagt accgggcaat
    10921 gctgaacacg ccccggttga acggcgcgat cctgccccgg cggccgcgcg tgccttcggg
    10981 aaagacgaac agattgcctc ccgccgccag ttggcgtttg atgtcttcca gatggttgat
    11041 catcgccgga ccgtacattt ccgttgggga ggagggcaca taacccgcct tgcgcagaaa
    11101 ccagccgaaa atggggacgc tgaaaaaggt gttcttgacg atggtggtct gccgggggaa
    11161 aagggacacc agcaggatgg gatccagata ggaaaggtgg ttgcagacga tgatggagga
    11221 gcgaagattc cggacgtctt cggaaacggc gaatcggacg ccgggcgaca gccggttcac
    11281 cagggcgaaa aaaactttca gatgaaggtg aaacgatttt tgcaagacgg cgtccacggc
    11341 cttcagcctg gaatagcagg ggatgaacaa aacaaacatc agcaccagat agcccagcat
    11401 gaaatagacc cagagcccca acgtgagaca gaaattgatg acgcggcgga aaaatcgtgt
    11461 gaggaacatc gcggtcaggt caactttcga atcatgaggc aggtgttcac gccgccaaag
    11521 gcaaaattct gaatggccgc ggttttgacg gggatttcct ccagcttctg gacatgccgg
    11581 atcatcgcgc agcgttcgtc gatttcttcc aggtgcagcg tcggggcgat aaagccctcc
    11641 tgcatcatat agatcgtcaa aaccgtttcg accgcgccgc aggccgccat ggtgtgcccc
    11701 atgtagctct tgagggccac cacccgcggt tgtgtgccga aaaccctgcc cgtggcctgc
    11761 gcctctatga tgtcgcccat cttggtggcc gtggcgtggg cgctgacgag atcgacgtcg
    11821 gaggcgttga tctgtgcgtt ttccaggccc agccgcaggg ttttggtgat gccgtcgaga
    11881 ttgggcagaa tcaaatcgcc gccgttggag ttgcaggcaa aaccgatgac ctccgccaga
    11941 atcgtcgcgc cgcgtttcct ggcgaactca tattcctcca gcatcacggc gcccgccccc
    12001 tcggcgacca ccagaccgtc gcgcgcacgg tcaaagggac ggggcgtctc accgggccgg
    12061 tcgttgaagg cggtggaaca ggcgagcaga ttgtcgaata ccgccaccgt gatcgtgtcg
    12121 tattcgtccg cgccgccgca gatcatggcg tcctgcaggc cgtacttgac cgcttcgtat
    12181 ccgaagccga tggactggct tgccgtggtg caggccgtcc cggaggaaat gatgcggccg
    12241 gtgatgccga acatcttgga aatattgacg gcggttgtgt gcaccatgga tttcaggtaa
    12301 tcaacggccc cgatgccctg aaacgtcgcc ttgtccttcg cctcaaagaa aatcctgtat
    12361 atgtcccttt gcacggtggg agaaccgtgt atcgacccga acgcgatgcc cagccggccg
    12421 cccgtgacaa aatccggcgg caggccggcc tgttccagga cttccctggc aacctggcag
    12481 gcgtagtagg aaacgggccc catggtcttg cggtactgcc ggctgaaatc ataggcgatc
    12541 ggatagccga cggtcccgta aacgcgggaa tgaataaaac ggctgataag atggtcttcg
    12601 cgcagtgccg caacccccga ttttccgttc acgaggcttt cgacgatggg ctttcggccg
    12661 tgcccgatgg gggagatggc cgaagccgcc gtgatgacca ctctgcgctg gggcatttat
    12721 gctttctccg cttcgttctt ccggtgatgg gcgagcattt ccacgtagtc gataatctga
    12781 ttcagcgtcc gaatgtccat ggctttcttt ttttccgcct gcgtcagttc cgttcccaga
    12841 aatctttcga tttccagaag cagttcaatg gcgtcgatgc tgtcaaaccc gtaggcttcc
    12901 cgcaggttca cgtccggttc ggggttttcc acctcgaatt ctctaagcag gacggcgcgg
    12961 atttcctttt caatttcctg gcgtgtcata gatctccttt agcggcaaaa gtgcccggaa
    13021 agctagcata aatcggcgag gtcgtccagc attagagtaa ccgttcgagt tccttgccga
    13081 aacgctcatt ccccgccatt ttccgcgcca cgccgagaga ccccagaatg caccccatca
    13141 ccccgggagc catggcgttt tgtccggcga ggtagagccc gcctatggga aggttgccga
    13201 gcgaggcgat tttcagcagg tgccgcgagg agcgcagaac accgtagcag gacccttccg
    13261 gacagctcac atggtcgcgc agcgagagag gcgtgaagac gtccagcagt tgaaggtttt
    13321 tcagggggcc gaagacggcg gacgattcgg caacgagcgc ttcggccagc gcctgttttt
    13381 tctgttcgta ggcctcgccg cgccgtctcg tcagggtgtc ttcccacgga agccagtccc
    13441 ggtaatgcga gcccgtgatg atggacagca ggttcgtatt ccgtccgtcg cctttgagca
    13501 cctggcaaaa aaagccgttt tcgatttctc cgctgccgcc ggcccgaagc cggtacaggt
    13561 tgtaatcgac ggcgggatgg gcctcggcat ccacgcccgc ctgaacggca aggacgccgc
    13621 cggtttcctc gaggcccgcg attctctccc gctgggactc tctgagcgcc tccggatcca
    13681 gcagtccgat gagaaccttg ggatgaacgg ccgccaccac cgagtccgcg ggcagtgaat
    13741 ccccctcttc aagatcaact ccggccacgc gtccctgttt ggtgcggata tgccggacgg
    13801 ccgaggagag cctgagcctt ccgcccagtt gtgaaaaccg cgccgcgaag gcgtccgcca
    13861 gatcccgccc gcttttcgga cgccacgatg aaaacaggta tccggccaga accatttgat
    13921 gaatgatgac cgggcaatct ttgagttcga cgccgacgag ctggcagggg gccgccagaa
    13981 cggcccgcag tttcgaagac gcacccagtt cgcaaagaag ctctcccatc ggacggaagg
    14041 aatccatgtt ggcaaaggga ctgccgcctt caaagaagaa gggcgatgcc gccatgacgc
    14101 cggccgtccc gcgcagacct ttcatcagcg cgtccagggc tgcgccgtcg gcccgacagg
    14161 cttcccgaag atttttttcg taggcgtcca gaccctcggg caggtcgaaa acgaaatcat
    14221 cgaaaagata gcggtcgatg acggccctgt ttttcatgcg ggaaaacagg ctgtccggat
    14281 cgatgcccag cacgcgaaac attttgccca gcggttcgga aggccccagc gcgccgacgt
    14341 agtgcacgcc cacggggcag tcgatgcccc ggcgggaata cgagcgcatc aggccgcccg
    14401 gctcggcgtt tttctccacg accgtcacgc gaaagcccag aagcgaaagc agaatgcccg
    14461 cggaaaggcc gccgacgccc gagccgatga tcaccgtatt ttttccctct gccggttttc
    14521 gcatgccgtc ctttcctgaa aattatttcc ccgaaagcgc ttcgtccaaa acggcttccg
    14581 gttcgaccag cgtcatcgac agagagtagc cgatccattc cgacgcctga agctcaatgg
    14641 cggcgtaagg attggcgcct gccgccagcg ccacgctgcg cctgagcgtg ccgccttttg
    14701 agtagcgacg gatttgaacg cgcccgtcgc cgtcttttcc gacatccagc cacccgccgc
    14761 cctgcaggcg gcgccggcac acaaaccggc ccgtgtccga aaaacccgcc cggtcgaccg
    14821 gctccggcgg ctcgaaaaaa atcagttcaa tatcgtcgag caggccgcgg gcgaattctt
    14881 cctcgtcaaa gggcggcagc gcccggctga tttgggtgcc ggaggggttt cgcgaggctt
    14941 caaagaggac catgccctcc gggctcatca gggcgcagga aagagtcctc gatgccgggt
    15001 ccgccaccgt cacgccgatc atcacggccc ttgtttcgcc ggccgcggcc gcttcaaccg
    15061 catggatgaa gagggttttc cgggcgacga acggcgaagg gcaggcgatc accttccccg
    15121 caacaacggc ggacggccgg atttccggca gggacgaaca ggacgccagc agcaggggaa
    15181 ggatcagaag aagacggagg ttcatggaat gtcctgaaag gtgccggggg gcagggtctg
    15241 gttcacgacc gtgtggagaa agtcaatcct tgtttcggcc gcggcgccct ccacaatcct
    15301 cacggttttc accacggcgt ctttccggga aacggtgatt tcgatttttt caatcaggcc
    15361 ggccgtgttt ttccccgtcg gcgtcagaac aatctgcgtt tccggccctt cctgaacacg
    15421 ggcggcaaag gacggattgg cggtgaattt ccccttcatc cagcccgcca cttcgtcgag
    15481 cacgattttc atcgccaggg cgccgcccgt tttgtcctcg atcattttgc cgccggaggc
    15541 aatgtagcgc ttcgtgctcc cttgattcga gatcacgatg ctccggatcg gctttacata
    15601 ttcccagcgg atggaatccg gggccgcata atagaatttc cccttcgaga ccaggggcgc
    15661 ggacaggatc ttcatggatt tcttctgaac aaagtctgcg gaaagagacg agatgccgga
    15721 cgcgtccctg cgcagccggt caaagtcatc cgccccggcg gcgtcgggaa acgccgtcag
    15781 caacaaaaac agcaaaatca agagccgtct gccagcctgc ccgcgcatca aaacattcct
    15841 ccgttgacgg aaatgacctg cccggtgatg taggaggcgt cctcggagca cagaaagcgg
    15901 atcacgcgcg cgacttcttc gggtttgcca acccttgcca tcggaatgac gcttttaatg
    15961 atgctttcgt ccatcggcat gctcttcagc atatcggtgg cgatggggcc cggcgcgacc
    16021 gcgtttacgc ggatgccgaa gcgggcgact tccttggcca gcgagctggt ggcggcgttg
    16081 atgccggcct tggccgccgc gtagttggcc tgcccgcggt tggggaccat ggaggaaatg
    16141 gaagaaacgg acacgatgga gccgcgtttt ttccggacca tctcgcgcag gacgggtttc
    16201 gtgacgtgaa aaaacccgtt gagcgttgtc tggattacgc gatgccactc ctcttctccc
    16261 atcatcatga aaaggccgtc cgccgtgacg ccggcgttgt tgaccagcac gtcaatccgt
    16321 ttgtgctcct gcagaatcgc ggccatggct tccttcaccg ccgccgggtc ggacacgtcg
    16381 aattttctca tttcgccgga cccgccggcc gcctgcacct gcgcgagggt ttcagccgcc
    16441 gcggcttcgc tggcgttgta gttgatgatc acgtgacagc cggtccgggc cagttccacg
    16501 gccgtggccc gtccgatgcc ccggctggcg cccgtcacca gcgcgatttt tctttcttcc
    16561 atgatgtttt tttctccttt ggtgaatttt acccgtcaat cctggttgag acgaacgacc
    16621 tggagaacag ccgtaagcag tgtttcattt tccgccgtga cgatgccgtt gatgacggca
    16681 taatcgtcaa aggaatacag gcttttgatc gaaaccgtga tccgggtttg cagggcgaga
    16741 agctcccggt gaaattccgc ggacttgatg cccaccagcc agcctttgat gctgtcctgt
    16801 ccccgtttct tccgtttgta gccttccacc agcgcggcgg tctgggcgac ggcttcgacc
    16861 aggacgagag agtgaacggt tcttccgtcg aaaaggggcc agcgctcatt gaccacggcg
    16921 gccgcggtgc cggcatcatc ctgcagctct atggcttccg tgatgatttt gatcggttca
    16981 cgatgcggaa tcacggattc gatgtcgatc atcggcggcc cccttttcct gctttaaatt
    17041 aaagagaaaa tccatcgaaa gtcaatcaaa aacattgcgc gccgcccgcc gtatttcgtg
    17101 taaaacgttc attgcttttc ggctttgttt cttatagaac atccaaaatg gtttcgcgca
    17161 ggtacccatg tccgcagagc tgaaagaccc ttttatgacc gcctgccgga agctcctgtc
    17221 tgtgccgaag gcgctgtgcc ggaaggaatt cacctgttcg ggagacacct acggagaagt
    17281 tttttccctg gcggccggtc tgaagaaaac gctttccgga cgcggcgcgg aaaaatcagt
    17341 ctgcctgtgc acggaaaaca aggccgtgtt cgccgcctcg cttctggccg cgctggcggg
    17401 cggctgcaat ctgatcctgc cctattcgtt ttcgacccac gcgctcctgg agatgtacga
    17461 ggcgacgggc tttgacgccg ccattgccga tcgcccggaa gaaatgccgg gcgtggaaat
    17521 gatcacgccg acggcggggg acccagcgga tctgaatccc gggggcctga tcgatcccga
    17581 ccggtctttc ctgaagcttt ttacgggcgg atccaccggc caaccaaggg tatggtccaa
    17641 aaccccgcgc aatctgttcg ccgaagcttt ttatctgcgc gatcattttg gcctgaccgg
    17701 cgacgacgtg tttctggcca ctgttccccc ttaccacatt tacggtcttc tgttttccat
    17761 cctgctgccg ctggttgcgc gcgcccgcgt gctcccggaa atctacacct tcccgcagga
    17821 aatcctctcg accgtcaacc gccacagggc caccgttctg gtcagtgtcc cgatttccta
    17881 ccggtcgttg cgggtggaca acctgtcggt cccctcgctg aggctcgcgt tttcgtcctc
    17941 cggcccgctg gatcggtccg acgcgcggta ttttttgaaa aaaacgggcc tggggatcca
    18001 cgagatctac ggttccaccg agacgggcgg cgtcgcgtcg cgcagcgtga gcgaaaacac
    18061 ggaaagctgg cagcccgccg gccccgtttc ctggcggatg ttcgggcggc ggctggcggt
    18121 gcgctctgat ttcgtgtcga gcgagatgga aagggacgcc gaaggcttct gcgtgacggg
    18181 cgatgaagtc cagccggacc gggacggccg gtttcttctc ctggggagag ccgacgggat
    18241 tgtcaaggtc gccggaaagc gcgtggacct gcaggaagtc cagaacaaaa tccagtccct
    18301 gccgaccgtg cgggacgcgg tggtcattgc gcttccggcc gaaaagggcc gagaaagcga
    18361 aattgcagcg ctggtcgcct gcgacctgac ggaaatccag atcaaaaaaa tgattctgga
    18421 aaagctggaa ccgtacgcca tgccaaggcg ctttcacatc gtttcttcca tccggcggac
    18481 cgccaccggc aagatcgatt atcgccgcgt cgaacagatc tttcttcggg aaaaaggaaa
    18541 aagccaatag cggaaatggt gaatagtgga tagtggatag tggatggtgt ccctcccttc
    18601 ggcaggctca ggacaggcta ggctcgggat gacatccttc tttgtcatgg tgagcctgtc
    18661 gaaccatgac actttccact ttccactcgt tgctcgtcac ttttcactgt cctctctgcg
    18721 cggaaaaacc ttgacttcac cggcgaacgt tcattatact tccggccgat tcaggattca
    18781 tgcattcgca gcggcggccc gtcatcactt caaccacacg aacaactttt gtcttttctg
    18841 atcatgggga aagggaggaa aaaatggcgc gttttcgtga gtatgaaaac tacgatggtc
    18901 ttgggctggc cgagctggtc agaaaaaagg aagtgacccc gccggagctg tgcgaagaag
    18961 ccgttttacg catcgaccaa ctcaatccga agctcaacgc ggtcatttac cggatggatg
    19021 acgctgcgcg cgccgcggcc gcgggcccga ttccggaagg ccctttttcc ggcgtgccgt
    19081 ttttgtgcaa ggatctggtt tccgccatcg cgggcgtgcc catgaccaaa ggcagcaaat
    19141 cctgccgcaa ttacgtgcct tcgtacgaca gtgaaatggc caggcgcttc cgggcggcgg
    19201 ggctgatcgt tctgggaaaa accaacacgc cggaattcgg cctgatcggc ctcaagccca
    19261 cccgcggccg caatccgctg gggcccgaat cgggcgaagt ctggcagggt gcggaggtgg
    19321 agggcgcgct gacccgctcc gtgcgcgatg cggccgcgat gctggacgcc acaaacggcg
    19381 cagattccgg cgctccctat attttgcccc ggccggcaag gccgtatctg gaagagacgc
    19441 gccaggatcc ttcgcggctg aaaatcggat tcacggtgga atcgccgctg aaaaacgccg
    19501 ttcatcccga atgtgtcgag gccgtgaaaa atgcggccaa gcttctggag cgcctcgggc
    19561 acaaggtgga agaagccagg ccggatattg acggcgaggc gctgggcaga agctacatga
    19621 tgctgtattt cggcgaggtt gcagcggaca ttcgggagct cgcaagcgtc gtgggccggc
    19681 cgctcaccca ttccgacacg gaggaagcca cctgggccct gaatctgctg gggaagactt
    19741 tttccgcggg cgacttcgtt ctggccaaac ggcagtggaa tacttttgcc cggcaaatgg
    19801 ccaaatttca cgaaacctac gatctgtatc tgacgccgac gattgcttcg ctgcccccgc
    19861 gcatcggcga aatgcagcaa aagccggccg aactggcttt cctgaaaatc atcaacggcc
    19921 tgaacctggg gggactgctc aaagcgacgg gcatcgccga tatgctggcg accaagggcc
    19981 tgtccaaaat gcctttcacg caactggcca acctcaccgg ccagccggcc atgtcggtgc
    20041 ctctccactg gagcagggaa ggactgccct gcggcgtcca gtttgtcggc cgcttcggcg
    20101 aggagtcgct tttgtttcaa ctggccggcc agctcgagcg ggcgcagccc tggtttgacc
    20161 gcagaccggc cctgacgaaa agctgacggg gaagttcaaa cacccttctt ctcaaaagcg
    20221 gaaaagcaac gccgcagacg ggcaggcgct cctgcatgaa cggaattttc gcctgcccgg
    20281 ccacgaaaag ccggtaactc agtattattc catatttgtc tggtccggac cgtcaagatt
    20341 ctcagccaaa aaagaatttc gccaattttc aatgatttta ttgacatttt ctataaatca
    20401 cgtatagtcg gcccagttta cgttaagtga tccgacgaag gatattcgcg cgacattggc
    20461 gctaagccgg agccgggcgt ggttccgcgt gacgctgaaa aatattacag gtgaggtggg
    20521 ttatgtcagg ttttgtgaac aaaacgatgg gagacgtgat tgaggaaacg gccggccgtt
    20581 ttcccgatca tccggcactg attgctccgc agtttcaggt ccggctcaat taccgcgaac
    20641 tttatgaaca atgccgcaaa accgcccggg ggctgatggc gatgggcgtc cgtcgcgggg
    20701 accatgtttc cgtctggacg accaacgtgc cggaatgggt gtatctgcaa ttcgcgctgg
    20761 gcatggtggg cggcattctg gtgacgatca ataccaatta ccagtctcac gagctggaat
    20821 acattctcag gcagtccgat tccaccaccc tgttcctgat cgaccagtac cgggacacca
    20881 gcttttacga aacagtccga aaggtcgttc cggagctggg ttcctgcgag cccggaaaga
    20941 cggcgtccgc caagctcccg gtgttgaaaa acgtcgtgta tatcgggaac cgggaaagcg
    21001 cgccgggcat gttcaaattt tccgatctga tcgccatggg ggagagcgtt tcggagcagg
    21061 agctgggcga gcggatgagt tcgctcaccg acgacgatgt catcaacatg cagtacacgt
    21121 cgggaacgac ggggttcccc aaaggcgtga tgctgaccca ccacaatgtc atcaacaacg
    21181 cccgcatggt cggcgacgtc atggggctga cggacaagga cacgctgctc attcaggtgc
    21241 ccgtctttca ttgcttcggg tgcgtcatga gcacgctcaa cagcgtttac c
//
LOCUS       DAOSIM010000011        21006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_2783_length_21006_cov_8.046537, whole genome shotgun sequence.
ACCESSION   DAOSIM010000011 DAOSIM010000000
VERSION     DAOSIM010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 21006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21006
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2783_length_21006_cov_8.046537"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="PLR47_01075"
     CDS             complement(<1..909)
                     /locus_tag="PLR47_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOG11554.1"
                     /translation="MKIGILTIGNELMNGRIADTNSSFIAREINLQGWTVETMMSVGD
                     DFEIIDNRLHYLLSFTDAVICTGGLGPTSDDITTAAVARAFGRQLVLDEKVLTYLKDI
                     FTRFNLRWTGNNAKQALFPEGAEVIPNPVGTAAGFALPVGEKLVMVIPGVPSETRRMV
                     PEGVIPVLRRRFPQAVLQTVKQTIKTFGLGEAMVDQKLADVDFDALGVSVGFYPVFPE
                     NHLVLITHSDSLEQAQKNMQQAVDKVTVRLSDCIFSFGEESLEERIAAELTAKKLTIA
                     VAESCTGGLIADRLTDVSGSSVYFERG"
     gene            complement(919..1383)
                     /locus_tag="PLR47_01080"
     CDS             complement(919..1383)
                     /locus_tag="PLR47_01080"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOG11555.1"
                     /translation="MTTSQKDKWIRILATGFGTGLSPVAPGTAGTLAGVLICLASSPL
                     PWLFRLFFVVAISAVSIYVAGEAEKLYGNTDDQRIVIDEIAGYQVAMLPVAVTGLHLL
                     LAFLLFRIFDIWKPFPLKHFQKFPGGWGVVVDDLGAGVYAGILMLIFVFLTP"
     gene            1556..2047
                     /gene="mobB"
                     /locus_tag="PLR47_01085"
     CDS             1556..2047
                     /gene="mobB"
                     /locus_tag="PLR47_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HOG11556.1"
                     /translation="MIPIVSIVGKSNSGKTTLLEKIIADLTHRGYRIATIKHNRHGFD
                     IDHEGKDSYRHKKAGAHTTVVSSPHQLALVQDVDHDHSFEEIRDKFISGADIILTEGF
                     KVNDYPKIEVFRTELKRDLISSRKDGLVAVAADVKLDIDVPCLDINDPKTVADFIEIH
                     FLK"
     gene            complement(2181..3089)
                     /locus_tag="PLR47_01090"
     CDS             complement(2181..3089)
                     /locus_tag="PLR47_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOG11557.1"
                     /translation="MKKMMWLKAVVLAVLLLLPLALTAPVQAARSIDPIVSTEWLQNN
                     LNNANLVIVDVRKVEEYKAGHIPGAVNVVYGTWAVKKGDLLNELPAVDDLADAIGSAG
                     IDQNSLVVIVGKTDKVPDQFDMTRVAWTLKYLGVPNVAILSGGQNQWLKESKPVLQDM
                     TRPKEKEFAASVNKGLFVDKASLAGKIGKALVIDTRAPAFYEGKEKLPFVPKLGRIKG
                     AVNLPVGQLYTPEGLYKDKAALAALAQKAAGSDLDKEIIVYCDTGKTCTGWAFIMTDL
                     LGYKDVKVYDGSSMEWLADPNVPTEP"
     gene            complement(3155..3874)
                     /locus_tag="PLR47_01095"
     CDS             complement(3155..3874)
                     /locus_tag="PLR47_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOG11558.1"
                     /translation="MTEKILSEKNRVVRHSRLELIEHWAIAISGLILLATGIFELPVA
                     KRYYIVTVPGLRWTADFITSLYLHYAAALVFTAAGIFHLAYHGMRGETGLLPKKGDLS
                     ASVTVIKSLFGFGQEPPMHKYLPEQRLAYAGMAWIIILLIVSGLIKTYKNLYAPDLSL
                     AVVLTATWIHNICFVLFFLAFLAHMAAILLRPNWPMVRGIFTGKVRLDYARHRHALWI
                     ADLEKTKEPLIPDRDLRCGKV"
     gene            complement(3867..4871)
                     /locus_tag="PLR47_01100"
     CDS             complement(3867..4871)
                     /locus_tag="PLR47_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG11559.1"
                     /translation="MDSGKASKITRRSFLRISAGAAVLTGVHVPKTARAESAGSLSTL
                     IDLSLCDGCADRNMPACVQACRSINANKIPQVAENIPEPWPRKTIEDWSKKQGVFNRL
                     TPYNYLYVHRADVMVDGRSQTLFVPRRCMHCDNPACATICPFSANHKNKNGAVVIDPD
                     QCFGGAKCRDVCPWEIPQRQSGVGIYLHVLPDFMGNGVMYKCDLCNDRLKEGKKPGCI
                     EACPRGAMLIGPRKEIEAEAQARAHQINGHIYGQKENGGTATLYVSPVSFELLNQTMT
                     KKPGQPDMKPGVKRRMAGTDPLGKAVLASPVLGLALAGAAGWFSRRKERQARQEEDRD
                     "
     gene            complement(4884..5177)
                     /locus_tag="PLR47_01105"
     CDS             complement(4884..5177)
                     /locus_tag="PLR47_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOG11560.1"
                     /translation="MFIYTPTGYIMDEMRIKVLNRLKRIEGQIRGLERMVEQEAPCAD
                     VLTQLNAASAAMKKTGVVIIQANMRRCIQEAADDQEKSIEEFQNALTRFIGMS"
     gene            5288..6631
                     /locus_tag="PLR47_01110"
     CDS             5288..6631
                     /locus_tag="PLR47_01110"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOG11561.1"
                     /translation="MDPACDLKNIYRDAVREVDPAGLIVSRVRKEGRKLALTGQEAEW
                     SEDLSSYRQVMVLGIGKASARMASAMESLLDEDLTSGFVITKYGHGLDLRKVKVHEAG
                     HPVPDENSLKGARILTDMARAADEHTLIIHLISGGGSSLFCLPADGISLDHKRRTTQA
                     LLASGAGIDEVNCVRKHLSKVKGGGLARIASPARLINLILSDVIGDRIDTIASGITAP
                     DPTTFQDALSIVHRYGLDGRLPEAVMDRLTRGARGEVAETPKAGNPAFCNSINLILGN
                     NALACGAAREAAQKLGYDAQILSTTLSGEAVEAGKCFAQLAKAIKEGKSLKEKPAIII
                     AGGETTVTLRGKGKGGRNQEMALAFALELHRILPDSSNIFFLSAGTDGGDGPTDAAGA
                     LVTPALMEKMKNISLEAASSLQDNDSYSFFQKEGGLFITGPTYTNVCDIQILAVT"
     gene            6787..7956
                     /locus_tag="PLR47_01115"
     CDS             6787..7956
                     /locus_tag="PLR47_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOG11562.1"
                     /translation="MNTYIDYSKRIRKIQEEMKKLDIDLFVGTRTVSLSYVAGAFVPW
                     RSAVIVSADGYAGLITMLLDCERLKNESWLSSISPYAPLPGMDLLDLIVHFVTQNKAQ
                     KGRIGVELGHSPRGNTGYLFATEYEFLKKALPDATFVNAVNVVDRACYIKEPGEIKLL
                     RQAAAIADSGIKCVRDALDVGMMETEIAGIGEMELRRLGSEYHWAVTGSSEVASGYRA
                     TYAMNGTTQPTQKMVQEGENIIVDLHPLYQLYPSDLAHNFILGKPSSEQRKLADAYLK
                     TAETIVRNFKAGNKVGDISKTVMDTVTELGYAPYTIPSFGHGLGVFGHEWYPAIVNND
                     EFRDVVLEENVVEVAFLAMTVPGVCGMRLECPVLVTKDGGEMLCKTPLALTVLEV"
     gene            complement(8218..8553)
                     /gene="trxA"
                     /locus_tag="PLR47_01120"
     CDS             complement(8218..8553)
                     /gene="trxA"
                     /locus_tag="PLR47_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOG11563.1"
                     /translation="MGDKANVKHSDDQHFSSTVLKQDGPFLVDFWAPWCGPCRALSPV
                     LEELADLYEGKIDIVKVNVDENPETASKYGVRSIPTLLLIQNGNVRETQIGLLSRQQL
                     AAMIDRNLN"
     gene            8683..9360
                     /locus_tag="PLR47_01125"
     CDS             8683..9360
                     /locus_tag="PLR47_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOG11564.1"
                     /translation="MDILERMKKIDLFQGVSFESLRVLAAQALYKKYAPQEMVIGEAD
                     FIRSFYIVLEGRLKLYKSSPEGREQTIQLLEPGDPFGLCTAFATDAFPASAMAMEPSG
                     VLLIPGTLVEAVALKEPRLLLNIIQILSRRLQHSMALIESLALKEIPARLASFVLHSL
                     LQRPEGAARLDLVITQRELAKILGATPEALSRALRKMTDEGILRASGRTIHVLNRSAL
                     QRLAEGE"
     gene            9528..10424
                     /locus_tag="PLR47_01130"
     CDS             9528..10424
                     /locus_tag="PLR47_01130"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HOG11565.1"
                     /translation="MKRIFLPQFVILALFIVLLPAAGLARDITPIVSTDWLLTNLKNP
                     KLVVLDIRRVEDYREGHIPGALNAFYGAWAYMKDGLYASLPEKDEIDDTISYMGINFD
                     RWVVVTGCMDTPRLSYQSARVACTLQYAGIKNVALLDGGTNKWIAEKKPLSTRVEKRK
                     AGKFKGKYTSEKFADKNYVKERLGKLILLDLREREFFTGEKKMDCVPMRGHIPGAFNM
                     PTSCAFNEDRTFKTKEELTAIAEAAAGKDLNAEIVTYCDAGQCCPTWSYLLTEVLGYT
                     NVRLYIGSMQEWMQDPKAPVER"
     gene            10505..10681
                     /locus_tag="PLR47_01135"
     CDS             10505..10681
                     /locus_tag="PLR47_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11566.1"
                     /translation="MKNRRTFLLAFLAVLIAALYGTAVFAATPNKCIQCHTNDALMKS
                     LHKPPVLPKSDGEG"
     gene            10715..11605
                     /locus_tag="PLR47_01140"
     CDS             10715..11605
                     /locus_tag="PLR47_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11567.1"
                     /translation="MYKGYVVDKALLDKDPHFKAGCVLCHAGNDGELGKEKAHLGMLK
                     KPSDNLRSCGVCHKKVAANYAKSLHYTTIGQRTGVMSRFSAAELKTFDEKVFEKSCRS
                     CHASCGDCHVKGAPVGGISICHKKAEFHGDGTAYKSKNEIPQRPACKSCHPNGKEAKM
                     ETQVAHRQHEGKVSCYGCHSGSAYRNCNDCHGGHAAASPGMILGRSPRDKKVLTTLRL
                     IPTVRDTFAPAGIKMEHFDAVPNYWDTPAHNIKKRTDRTRKCDACHVDRKNFLTKETM
                     IKNGAKANEDLIFVPKAIKN"
     gene            complement(11773..12000)
                     /locus_tag="PLR47_01145"
     CDS             complement(11773..12000)
                     /locus_tag="PLR47_01145"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOG11568.1"
                     /translation="MTTGNDFAVVLFESVSHALGAEKIVKGSGIGCKLIHVPRHLSSD
                     CGICLRFHAADRERVEALLRGKLHFFEIKLL"
     gene            complement(11997..13172)
                     /locus_tag="PLR47_01150"
     CDS             complement(11997..13172)
                     /locus_tag="PLR47_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOG11569.1"
                     /translation="MKTIYLDNAATSFPKPEAMLSAMTAYQRNVGANPGRSGHGLSID
                     AGRIICEARESLALLFHVEDPLRIVLTKNSTEALNIALQGGLRPGDHVITSGMEHNSV
                     MRPLRFLEGRGVELSVVPCSPRGEMDPEDLKKVLRKNTRIVVVTHASNVTGTILPVEA
                     IGELLQDRPDVMFCVDAAQTAGALPIDVEKMNIDLLAFTGHKSLFGPQGTGGLYIREG
                     LDQRIAPLMMGGTGSRSEFEEQPEFMPDRYESGTPNTIGIAGLGAGVSFVLEQTVEAV
                     RAREEELASRFLAGLQDLRDEVILYGPQDASRQTAVVSFNIKNVTSSDAADYFEEQRG
                     ILCRPGLHCAPSAHRTIGTFPRGTVRFSFGFFNTQQEVDDACEALRELVKRKGKPGS"
     gene            complement(13200..13412)
                     /locus_tag="PLR47_01155"
     CDS             complement(13200..13412)
                     /locus_tag="PLR47_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOG11570.1"
                     /translation="MSTKKVDARGLSCPQPVVLVDRAIADGNKDLEILVDNEVARENV
                     SRLLSRRGLKADIRQDGSDIIIRAAK"
     gene            complement(13434..14513)
                     /gene="yedE"
                     /locus_tag="PLR47_01160"
     CDS             complement(13434..14513)
                     /gene="yedE"
                     /locus_tag="PLR47_01160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HOG11571.1"
                     /translation="MKNIFSTRWGIILVGAVIGIGAALLQYFGNPPNMGICVACFERD
                     IAGALGLHRADVVQYLRPEIMGFVLGAFVAALLAGEYKPRGGSSPLIRFFLGVFAMIG
                     ALVFLGCPWRTLLRLAGGDGNAILGLAGLIAGIFIGVGFLKNGYSLGRSYAQKKAAGW
                     VFPALMIGLLLLLVFQVSFAPGGPIFFSAKGPGSQHAPLLISLIAGLVIGGLAQRSRF
                     CTMGAFRDVILIRDFHLISGVAALLVFAFIANMLLGQFKPGFEGQPVAHTDHLWNFLG
                     MTLAGLSFVLAGGCPGRQLFLSGEGDMDAAIFATGMIVGAAIAHNFAIASSPKGIAAF
                     GPAAVLIGFLFCLVVGFTQRDKVSA"
     gene            14772..16670
                     /locus_tag="PLR47_01165"
     CDS             14772..16670
                     /locus_tag="PLR47_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG11572.1"
                     /translation="MALIWVNHISISFGGPLLLDGASLQIEAGERIGLLGRNGSGKST
                     LMKLLSGEIPPDDGEISRDKNIHVSVMPQEVPDLRGTVYDVVAGGCQEHLDLLHEYHD
                     LTLRLHSGGDHQLLKKIEQVQHRLEAVGAWQYHQKVEEVISRVSLDESSEFLLLSAGL
                     KRRVLLARALVADPDVLLLDEPTNHLDIDAILWLEEFLKSFEKTLMFVTHDRAFLQKI
                     ATRIVEIDRGKLFSFSCDYATYLERRQAMQEAEEKQWETFDKKLAREEVWVRQGIKAR
                     RTRNEGRVRVLLVLREERAQRREREGSVRLSVSEAQASGKLVVEAEKISFAYENKKII
                     DSFSTRILRGDRVGIIGPNGSGKTTLLKVLLGELPATGGKIRLGANIAMAYFDQLRAQ
                     FDETKSLRDNIADGNDTVFINGVPRHVVGYLQDFLFPPAQILAPVGSLSGGEKNRLLL
                     AKLFCTPSNVLILDEPTNDLDAETLELLEDRLLEYKGTILLVSHDRVFLNNVVTSTIV
                     LEGEGRLQEYVGGYDDWLRQRGEAKEKPAAAKEVKTKKEKQQREKTKLSFKETKELEE
                     LPPRIGKMEKEKAELLELLNSPDLYKTHNPARALDVNNRLAELETDLTAAYDRWDALE
                     ELAAKFSA"
     gene            16773..18020
                     /locus_tag="PLR47_01170"
     CDS             16773..18020
                     /locus_tag="PLR47_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOG11573.1"
                     /translation="MKYSYKTACIISFSVIILTFTSISAFGSEASGLFNIGNMSHDIV
                     MLIAYVLLALLFSFLCSIAEAVLLSITPSYIEEQKEKNPKRAAHLKRLKQDNVDRSLA
                     AILTLNTIAHTVGAIGAGAKATVVFGSAWFGVFSAVMTLMILFLSEIVPKTIGAIYWP
                     KLVGPTVIFVRTLIVTLFPIVWISERMTKFISHGKNLHIFSRDEFVAMARIGEQSGHI
                     DDNEARIIKNLFRLGSINVTDIMTPRIVITALSDDKSVADASEYIAKIPFSRIPIYEK
                     DIDNITGFVLRDDVLLRDNQERGNELLKTLRREILIVPESVVLTGLLENFLKGRQHIA
                     IVVDEYGGTMGLVTLEDLVETLMGLEIVDEMDTVVDMRVLARKKWEERARALGINSDI
                     DERDPFEKLNVLPNDSAKSDTRE"
     gene            18088..18600
                     /locus_tag="PLR47_01175"
     CDS             18088..18600
                     /locus_tag="PLR47_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11574.1"
                     /translation="MNLKNVNFPRQLEAAGLAEKIRLALAERQTKLWPEGKMEVPVLS
                     ISESLIATIRNARLQRRIRFGFDDILDKLAAEKKGIDEMLQKNKSQQNNRVSRLLLFS
                     NDGAERLYRHIEQALVEHRLRILGCQLDIDGKELGRLIAGRSAGIKVILVEHKDAVSD
                     VLRALAEGRQ"
     gene            18712..>21006
                     /locus_tag="PLR47_01180"
     CDS             18712..>21006
                     /locus_tag="PLR47_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOG11575.1"
                     /translation="MSNRIEVGFREGIRDALGEKTKKRIIDNLGLPVAGAATIEVYTI
                     EGELTAGELRRAAAGPLSDPVIQAYAINKPLAKNFDWLIEVGFRPGVTDNVGKTAREA
                     ITLLLGDSLGERKISVYTSRQYLVSGPISRADAQNIASGLLANDLIERHQVVGKAEFD
                     FTGGMPVHIPQVTGRDEPRVETINLTKLAAAALLKISQEKLLALNLEEMQAIARYFAD
                     PLIAKARGKFGLDENTTDVELECLAQTWSEHCKHKIFNSRIEYNDGRTKKIINSLFKT
                     YIKGSTAKIRKAKGKKDFCLSVFVDNAGVIKFNKDHNLVFKVETHNSPSALDPYGGAL
                     TGIVGVNRDPFGTGLGAKLIFNTDVFCFASPFHNKKLPPRILHPRRIYEGVREGVEHG
                     GNKSGIPTVNGSIVFDERYLGKPLVYCGTAGIMPARQNGKPTHGKEIKSGDVIIMTGG
                     RIGKDGIHGATFSSEELHEGSPVTAVQIGDPITQKKMTDFLLKARDRGLYRALTDNGA
                     GGLSSSIGEMATYSGGCEIDLSLAPLKYAGLQPWEILVSEAQERMSLAVDSKKVGKFM
                     DLAAKMGVEATVLGRFTNSGKFHVRYGEKTVAYLDMDFLHDGVPQMKLQARWKAPKHP
                     EPRFSAPADLGKALAQMLARLNICSKESVVRQYDHEVQGGSVVKPLVGAQNDGPSDAG
                     VVRPVLDSMEGVVVAHGICPRLSDIDAYHMTACAIDEAVRNAVAVGVDIDHLAGLDNF
                     CWCDPVKSAKTPDGDYKLAQLVRSN"
BASE COUNT         4956 a         5736 c         5811 g         4503 t
ORIGIN      
        1 gccgcgctca aaataaacgg aactgccgga aacgtcggtg aggcggtcgg caatgagccc
       61 tcccgtgcag gattccgcca cggcgatcgt caatttcttt gccgtcaact ccgccgcgat
      121 tctctcttca agagactctt caccgaagga aaagatgcag tcggaaagtc gaaccgttac
      181 cttgtccacg gcctgttgca tatttttctg cgcctgctcc agggaatcgg agtgtgtgat
      241 cagtaccaga tgattttccg gaaagacggg gtaaaagccg acgctcacgc cgagggcgtc
      301 aaaatcaaca tcggccagct tttgatccac catggcctct ccgaggccga aggttttaat
      361 ggtctgtttg accgtctgaa ggacggcctg cggaaagcgg cggcgcaaaa cgggaatgac
      421 gccttccggg accattcgtc gcgtttccga cggaacgccg ggaatgacca tcaccagctt
      481 ttccccgacc ggcagcgcaa atcccgcggc ggtgccgaca ggattgggaa tgacttcagc
      541 gccttcggga aacagcgcct gcttggcgtt gtttcccgtc cagcgcaggt tgaatcgcgt
      601 aaagatatcc ttaagatagg tcaggacttt ttcatccaga accagttgcc ggccgaacgc
      661 ccgcgcgacc gcggcggtgg tgatgtcgtc ggaagtcggt cccaggcctc cggtgcagat
      721 caccgcgtcc gtaaaagaca aaaggtaatg cagccggttg tcgatgattt caaagtcgtc
      781 gccgaccgac atcatggttt caaccgtcca gccctgcaga tttatctccc gcgcgatgaa
      841 agacgaattc gtatcggcga tgcgcccgtt cattaattcg ttcccgatgg tcaaaatgcc
      901 gattttcata aaaccgcctt atggtgtaag aaaaacaaaa atcaacatca gaattcccgc
      961 gtaaacgccc gcgccgagat catctaccac cacgccccag ccgcccggaa acttctgaaa
     1021 atgcttcagc ggaaaaggct tccagatatc aaaaatccga aaaagaagaa acgccagcaa
     1081 aagatgcagg ccggtaacgg ccacaggcag catggcaacc tggtagccgg caatttcatc
     1141 aatcacaatc cgctggtcgt cggtgtttcc gtaaagtttt tccgcctccc cggcaacata
     1201 aatagagacg gccgatattg caaccacaaa gaaaaggcga aaaagccagg gcagagggct
     1261 ggaagccagg cagatcaaaa cacccgccag cgtgcccgcc gttcccggcg cgacggggga
     1321 aaggcccgtc ccgaatccgg ttgccaggat cctgatccat ttgtcctttt gagaggttgt
     1381 cacggacgtc ctttttaatc ccgttgtttg cgtgtagaac gattgttcta tactacaggc
     1441 ctttgcggga tgtcaagcaa tcttatgcac ggcctaggcc tttggccgac attgttggcc
     1501 aaagccggca aaatgtggta tgcaacggcc aagtaaagga atttcaggat aaaaaatgat
     1561 tcccattgtt tccattgtcg gcaagtccaa ttccggcaag acaacgcttc tggagaaaat
     1621 cattgccgat ttaacccatc ggggctatcg catcgccacc atcaagcaca accgccacgg
     1681 cttcgacatc gaccacgagg gaaaggacag ctaccgccac aaaaaggcgg gagcgcacac
     1741 caccgtcgtt tcatcgcccc atcagctcgc gctggttcag gacgtggacc atgatcactc
     1801 ctttgaggaa attcgcgaca agttcatttc cggcgcggat atcatcctca ccgaaggatt
     1861 caaggtgaat gactatccga agattgaagt gttccgaact gaactcaaac gggacttgat
     1921 ctcgagcaga aaagacggtc tggtcgcggt ggccgcggac gtgaaactgg atatcgatgt
     1981 tccctgtctg gacatcaacg accccaaaac tgtcgccgat ttcattgaaa ttcattttct
     2041 gaaatagcca taaagcttgc agaacttaca aacaatgctc tcaatcaatc tattgcggcg
     2101 ttttgtcttt gccgaaaaaa agcccggcgg tttgacggcc gggctttcgt ttgcttcagg
     2161 ggatgcagcg tgaggatgtt ctagggttcg gtaggaacgt tcgggtccgc cagccattcc
     2221 atcgaggaac cgtcatacac tttgacgtcc ttgtatccca gcaggtcggt catgataaag
     2281 gcccagccgg tgcaggtctt gcccgtgtca cagtaaacga ttatttcctt gtccaaatcg
     2341 gagccggccg ctttttgcgc aagcgccgca agcgccgcct tgtccttgta cagaccttcc
     2401 ggcgtgtaga gctggccgac cggcaaattg accgctccct tgatgcggcc taatttcgga
     2461 acaaagggca gcttttcctt gccttcgtaa aacgccggag cgcgggtgtc gataacgagc
     2521 gccttgccga ttttacccgc gagcgaagcc ttgtccacga acagaccctt gttgacggac
     2581 gccgcaaatt ctttttcttt ggggcgggtc atgtcctgca aaaccggttt gctttccttc
     2641 agccactgat tctgcccgcc gctgagaatg gcgacattcg gcacgcccag atacttcagg
     2701 gtccaggcca cgcgggtcat gtcgaactga tcgggaacct tatccgtttt cccgacaatg
     2761 accaccagag agttctgatc gatgcccgcg cttccgatcg cgtcggccag gtcgtccaca
     2821 gccggcagtt cgttaagcag gtcgcctttt ttaacggccc aggttccgta cacaacgttg
     2881 acggctcccg gaatatggcc tgccttgtat tcttccacct tccgcacatc aacgatgacc
     2941 aaattggcat tgttcaggtt attctgcagc cactccgtgg agacgatggg atcaatgctc
     3001 ctcgccgcct gtacaggagc ggtgagcgcc aacggcagca acaacagtac cgccagaacc
     3061 actgctttca accacatcat ttttttcata aatactacct cctctgtttc atttgaattt
     3121 ttgtctgtcg ctattttgaa gcctgtgacg gccctcaaac cttcccacag cgaagatccc
     3181 ggtcagggat caacggctct ttcgtttttt caagatcggc gatccacaaa gcatggcggt
     3241 gccgggcata gtccaaacgg actttgccgg tgaagatgcc gcggaccatc ggccagttcg
     3301 gcctaagcag aatcgccgcc atgtgcgcga gaaaagccag aaaaaagaga acgaaacaaa
     3361 tattatgaat ccaggtggcc gtcaggacga ccgcaagcga aagatccggc gcatacaggt
     3421 tcttgtaggt tttgatcagg ccggagacga tgagcaggat gatgatccag gccattcccg
     3481 cgtaagccag ccgctgctcc ggcaaatact tgtgcatcgg cggctcctgg ccgaagccga
     3541 acaggctctt gatgactgtg acggacgccg acaggtcgcc ttttttcggc agcaggccgg
     3601 tttcgccgcg cattccatgg tacgccaggt gaaagattcc cgcggccgta aaaaccagcg
     3661 ccgccgcgta gtgtagataa agggatgtga tgaaatccgc cgtccagcgc aagcccggaa
     3721 cggtgacgat gtaatagcgt ttggctacgg gcagttcaaa aattccggtg gccagcagaa
     3781 tcaggccgga aatcgcgatg gcccagtgtt cgatcagctc cagccggctg tggcggacga
     3841 cccggttttt ttccgaaagg atcttttcag tcacggtctt cctcctgacg ggcttggcgt
     3901 tctttgcggc gtgaaaacca gcccgccgcg ccggcgagcg ccaatccaag aacgggggaa
     3961 gccagcacgg cttttcccag cggatccgtt cccgccatgc gccgcttgac gcccggcttc
     4021 atgtccggct gcccgggttt tttggtcatg gtttgattca acagctcgaa cgatacagga
     4081 gacacataga gcgttgccgt gccgccgttt tctttctgac cgtaaatgtg gccgttaatc
     4141 tggtgggctc tggcctgcgc ctcagcttca atctcttttc tggggccgat cagcatggcc
     4201 ccgcgcggac aggcttcgat gcatcccggc ttttttcctt cctttaacct gtcattgcag
     4261 aggtcgcatt tatacatgac gccgttgccc atgaaatcgg gcaggacatg aaggtaaatg
     4321 ccgacgccgg actggcgctg cggaatttcc cacgggcaga cgtccctgca tttggcgccg
     4381 ccgaaacact ggtctgggtc gataaccacc gcgccgttct tatttttgtg attggcggaa
     4441 aacggacaga tggtcgcgca ggcgggattg tcgcagtgca tgcagcgcct cgggacaaac
     4501 agggtttggc tgcggccgtc caccattaca tccgcccgat gaacgtaaag gtagttgtag
     4561 ggggtaaggc ggttgaagac gccctgcttt ttggaccagt cttcgatggt ttttctgggc
     4621 cacggctccg gaatgttttc cgctacctgt gggattttgt tggcgttgat gcttcggcag
     4681 gcctggacgc aggccggcat gttgcggtcc gcgcagccgt cgcaaaggct caaatcgata
     4741 agcgtcgaga gagatccggc cgattcagcc cgcgcggtct ttgggacatg aactcccgtc
     4801 agaaccgcgg ctccggccga aatccgcaaa aaagaacggc gggtgatttt agaagctttt
     4861 cccgaatcca atttttcccc cctctaggac atgccgataa agcgggtcag cgcgttttga
     4921 aattcttcaa tgctcttttc ctgatcatcg gccgcctcct gaatgcagcg cctcatgttg
     4981 gcctgaatga tcaccacgcc ggtttttttc atggcggcgc tggccgcgtt gagctgggtc
     5041 aggacatcgg cgcagggcgc ttcctgctcc accatccgct ccagcccgcg gatttgcccc
     5101 tcaattcgct ttaatcggtt taataccttg attctcattt cgtccataat ataccccgtg
     5161 ggggtatata taaacactcc cgggtttcct gtcaaatggt ttttttacac tgatttttga
     5221 aatcacagct tgacaatgga agcccccttc atggtagctg tttgaaaaat atcgcgtgga
     5281 gaaagccgtg gatcctgctt gcgacctgaa aaacatttac cgcgacgccg ttcgggaggt
     5341 ggaccccgcg ggtcttattg tatcccgcgt ccggaaagaa ggacggaagc ttgcgctcac
     5401 gggccaggaa gcggaatggt cggaagacct ttcctcctac cggcaggtga tggtgctcgg
     5461 catcggcaag gcttcagccc gcatggcatc cgccatggaa agcctcctgg acgaagatct
     5521 cacttcggga ttcgtcatca caaaatacgg ccacgggctc gatcttcgga aagtgaaagt
     5581 ccatgaagcg ggccaccccg tgccggatga aaacagcctg aaaggagccc gcattctgac
     5641 ggatatggcc cgggcggcgg atgaacacac gctgatcatc catctgattt ccggcggagg
     5701 gtcatccttg ttctgcctgc cagcggacgg catcagcctg gaccacaagc gcagaaccac
     5761 gcaagccctg ctggcgtccg gcgccggcat cgacgaggtc aactgcgttc ggaagcatct
     5821 gtcgaaagtc aagggcgggg ggctcgcccg aatcgcgagt cccgccaggc tcatcaacct
     5881 gattttgtcg gatgtgatcg gcgaccggat cgataccatc gcgtccggca ttaccgctcc
     5941 tgatcccacc acgtttcagg acgccctctc gatcgttcat cgatacggac tggacggcag
     6001 gcttccggaa gcggtgatgg accgtctgac ccggggggcg aggggcgaag tggccgagac
     6061 gcccaaagcg ggcaacccgg cattttgcaa cagcatcaat ctgatccttg gcaacaacgc
     6121 gctggcctgc ggtgcggcga gggaagccgc gcagaagctg ggatatgacg cgcaaattct
     6181 cagcacaacg ctgtccggcg aggcggtcga agcgggaaag tgcttcgcgc agcttgccaa
     6241 ggccataaag gaaggaaaat ccctgaagga aaaaccggcc atcataatcg ccggcggaga
     6301 aacaaccgtc acgctccggg gcaaagggaa aggagggcga aaccaggaaa tggcactcgc
     6361 ctttgctttg gagctgcacc gaatccttcc ggattcttcc aatatctttt ttctgtccgc
     6421 cggcacggac ggcggcgacg ggcctacgga cgcggcgggc gcgctggtga cgcccgcgct
     6481 gatggaaaaa atgaaaaaca tctccctcga ggccgcgtcg agccttcagg ataacgactc
     6541 gtattctttc tttcagaagg aaggcggtct ttttatcacg ggtccgacct ataccaacgt
     6601 ctgcgatatc cagattctcg ccgtgacatg atgccgggcg cgcggcaaaa ggcgcggacg
     6661 gacggcgcag gcgaatgctg atccttcctg ccctacccgg gtttgcttga cttgacccgg
     6721 caaccggcct atactttttt ggaacgaaag ctgatttcaa tcagcggaca aagaaacgga
     6781 ggttctatga atacgtacat cgattattcc aagcgcatca gaaaaataca ggaagaaatg
     6841 aaaaaattgg atattgacct tttcgtgggc acgcgcacgg tcagcctgag ctatgtcgca
     6901 ggggccttcg ttccctggag aagcgctgtg atcgtgtccg cggatggata tgccggtctg
     6961 attaccatgc tgctcgactg cgagcgtctg aagaatgagt cctggctttc cagcatttct
     7021 ccctatgctc ccctgcccgg catggatctg ctcgacctga tcgtccattt tgtcacacaa
     7081 aacaaggcac aaaagggcag gatcggcgtg gagctcggtc attccccccg cggcaacacg
     7141 gggtatctct ttgcgacgga atacgaattt ttgaaaaaag ccctgccgga cgccacgttt
     7201 gtcaacgcgg tcaacgttgt ggatcgggcc tgctacatca aggaaccggg cgaaatcaag
     7261 ctgctgcgac aggccgccgc catcgcggat tccggaatca agtgcgtgcg cgacgccctg
     7321 gacgtcggca tgatggaaac ggaaatcgcc ggcatcggcg aaatggagct gaggcgcctc
     7381 ggcagcgaat accactgggc cgtcacggga tcgtcggagg tggcctccgg ataccgggcg
     7441 acatacgcca tgaacggaac gacgcagccc acgcagaaaa tggtgcagga gggcgaaaac
     7501 attatcgtcg atctgcaccc gctctaccag ctctatccct cggacctggc tcacaatttc
     7561 attttgggaa agccctcgtc cgagcagagg aagctggccg acgcgtatct caagacggcg
     7621 gaaacgatcg tccgcaattt caaggccgga aataaagtcg gcgatatcag caagaccgtg
     7681 atggataccg tgaccgaact gggttacgcc ccatacacga ttccctcgtt cggccacggc
     7741 ctgggcgtgt tcggccatga atggtatccg gccatcgtca ataatgacga attccgtgat
     7801 gtcgttctgg aggagaatgt ggtcgaagtg gcttttctgg ccatgaccgt tcccggcgtg
     7861 tgcggaatga ggctggagtg cccggttctg gtcacgaagg acggtggcga gatgctgtgc
     7921 aaaacgccgc ttgcgctgac ggttctggaa gtttgaggtc cgcaaagaga tgacggaaaa
     7981 gccccggcct ttttacccgc gaaggccggg gcttttgttt gaaagaatcc gctaccgcgg
     8041 agacgttttc ttttttctgt ccggagccgg acaggaaggc cgcattcagg tggatatgcc
     8101 gaatttcggc aacagccacg cctggagaaa aacccatact cctacaatca gcagtaaaat
     8161 caaccagtca ttcatgtttt tcctcctttc gccgggttgg cggccggttc caaccggcta
     8221 attcaggttg cgatcgatca tagccgcaag ctgctgcctg gacaacaggc cgatctgcgt
     8281 ttcccgaacg tttccgtttt ggatcaagag cagcgtcgga atgctccgca cgccgtactt
     8341 tgacgccgtt tccggatttt catccacatt caccttgacg atgtcgattt ttccttcata
     8401 caggtccgcc agttcttcca gtaccggact caacgcccgg cacggaccgc accagggcgc
     8461 ccagaagtcc accaggaacg gcccgtcctg ctttaacacc gttgatgaaa aatgctgatc
     8521 atccgaatgt ttcacgtttg ctttatctcc catcgttttt cccccgctaa tttattttgt
     8581 ttcatctttg gtctgtggta aaccattctt gcgttttgca caaaccatag catcggccgg
     8641 tcagcgcgtc attgatctga atcaatgccg ggggagcatc gaatggatat tttggagcgc
     8701 atgaaaaaaa tcgatctttt tcagggcgtt tctttcgaaa gcttgcgggt cctggccgcg
     8761 caggccctgt acaaaaaata cgccccccag gaaatggtga tcggcgaggc ggatttcatc
     8821 aggtctttct acattgtcct tgagggcaga ctgaagcttt acaagagttc gccggaaggt
     8881 cgggagcaga cgattcagct tctggagccg ggggaccctt tcggcctgtg caccgcgttt
     8941 gccacggatg ctttcccggc cagcgccatg gcgatggaac ctagcggcgt tctgctgatt
     9001 cccggaacgc tggtcgaggc cgtggccctg aaggagccca gactccttct caacatcatt
     9061 cagattctgt cccgccggct gcagcattcc atggcgctca tcgaatcgct ggcgttgaag
     9121 gaaattcccg cccggctcgc ctcgtttgtg ctgcattccc tgttgcagcg tccggaaggc
     9181 gcagcccggc tggatctggt gatcacccag cgcgagctgg ccaagatcct cggggccacg
     9241 ccggaagcgc tttcacgcgc cctgcgcaag atgacggatg aaggcattct ccgcgcgtcg
     9301 ggacggacga ttcacgttct gaatcgaagt gccctgcaac gtctggccga aggagaatag
     9361 cgacacgctg atgccaaaga cggcttttct gtaagggata agagccctga agagaacatt
     9421 gaactggcaa ttttgaaccc ggacatggta tactttacac gacgtaaaga cgaaacggtt
     9481 ttgacgatac gctcatcccg agcgcgggtt gacaaggagg aaatcgcatg aaaagaatat
     9541 ttttgcccca attcgtcatt cttgcattgt tcatcgtttt gctcccggcc gcgggtctgg
     9601 cgagggacat taccccgatt gtttccaccg actggctttt gaccaatctg aaaaacccca
     9661 aactcgttgt tctggatatc cgccgcgtgg aagactaccg cgaaggccac attcccgggg
     9721 cgctgaacgc tttttatggc gcctgggcct acatgaagga cggcctgtac gcgtcgctgc
     9781 cggagaaaga cgaaattgac gatacgatca gttacatggg tatcaatttt gaccgttggg
     9841 tcgttgtcac aggatgtatg gacacgccac gcctcagcta tcagagcgcc cgcgtcgcct
     9901 gtacgctgca atacgccggc atcaaaaatg tcgcgcttct ggacggaggc accaacaagt
     9961 ggattgcgga aaaaaagccc ctctccaccc gcgtggaaaa gcgcaaggcc ggcaaattca
    10021 agggaaaata tacctcggaa aaatttgccg acaaaaacta tgtcaaagag aggctgggca
    10081 aactcatcct cctggacctt cgggaacgcg aattttttac gggggagaaa aagatggact
    10141 gcgttcccat gagggggcac attcccggcg cattcaatat gccgacctcc tgcgctttca
    10201 atgaggacag gacctttaaa acgaaagaag aattgaccgc catcgccgaa gcggccgcgg
    10261 gcaaggatct aaacgccgag attgtcactt actgcgacgc gggccagtgc tgcccgacct
    10321 ggtcgtatct tctgacggaa gtgctggggt acacaaacgt ccggctttac atcggatcga
    10381 tgcaggagtg gatgcaggat ccgaaggcgc cggtggagcg ctgatccacg cccccgatga
    10441 atccgtttga caggctgaag ctgatttgtt acatcttgct ccactcttct cttgaggagg
    10501 tgttgtgaaa aaccgacgaa cattcctgct ggctttcctt gccgttctga tcgcagcgct
    10561 ttacggaacc gcggtctttg ccgccacccc caataaatgc attcagtgcc atacgaatga
    10621 cgcgctgatg aagtctctgc acaagccgcc cgttttgccc aaaagcgatg gcgaggggtg
    10681 agcgggtgcc cctccgccag ttcggccgga agaaatgtac aaaggatatg tggtggacaa
    10741 ggccctgctg gacaaagatc cccatttcaa ggcaggctgc gtcctttgcc acgcgggaaa
    10801 cgacggggag ctcggcaagg aaaaagccca cctcggaatg ctgaaaaagc cctcggacaa
    10861 tctgcgttcc tgcggggttt gccataaaaa ggttgccgcc aactacgcca agtcgctgca
    10921 ctacacgacg atcggccagc ggacgggcgt catgtcgcgg ttctccgccg cggagttgaa
    10981 aacgtttgac gaaaaggttt ttgaaaaatc ctgccgaagc tgccatgctt cctgcggcga
    11041 ctgccacgtg aaaggcgcgc cggtcggcgg gatcagcatc tgccacaaga aggcggagtt
    11101 ccacggcgac ggcacggcct acaagtccaa aaatgaaatt ccgcagcgcc cggcctgcaa
    11161 gagctgccac ccgaacggca aggaagccaa aatggaaacg caggttgcgc atcgtcagca
    11221 tgagggcaaa gtgtcctgct acggctgcca ttccgggtcg gcttaccgca actgcaacga
    11281 ctgccacggc ggtcatgccg cggccagccc cggcatgatt ctgggtcgca gcccgcggga
    11341 caaaaaagtc ctgacgacac tgcgattgat tcccaccgtc cgcgatacgt tcgctccggc
    11401 gggcatcaaa atggagcatt tcgacgcggt gcccaactac tgggatacgc ccgcgcataa
    11461 catcaaaaag cggacggacc ggacccgcaa atgcgacgcc tgccacgtgg atcgcaagaa
    11521 cttcctgacc aaagaaacga tgatcaagaa cggcgccaaa gccaatgaag atttgatttt
    11581 tgtgccgaaa gccattaaaa attaggagga gcgcactcta tttttagccc acaacaaaag
    11641 aaaaaaatgt agggcgtacc ttcaggtgcg ccaaatgaac cttgcaaaaa agcagggcgg
    11701 gtcttttgac ccgctctgct ttttttcgtt gctcattgcc cgtcgcttga aaatgggtct
    11761 cgtttttaat attcagagaa gcttgatttc aaaaaaatga agttttcccc gcaggagggc
    11821 ttccacgcgc tcccggtcgg ctgcgtgaaa gcgcaggcag atgccgcaat cggaactgag
    11881 gtggcgaggc acgtggatca atttgcagcc gatgcctgag cctttgacga ttttttccgc
    11941 gcccagcgcg tggcttacgg attcaaaaag aactacggcg aaatcgtttc ctgtcgtcac
    12001 gagccgggtt tccccttccg cttgacaagt tcccgcagcg cctcgcaggc atcgtccacc
    12061 tcctgctgcg tattgaaaaa tccgaagctg aagcggaccg ttccccgagg aaaggtgccg
    12121 atcgtccggt gggccgacgg cgcacagtga agccccggcc ggcagaggat tccccgctgt
    12181 tcctcgaaat aatcggcggc gtccgaagac gtgacgtttt taatattgaa cgatacgacg
    12241 gcggtttgcc gggatgcgtc ctgcgggccg taaagaatca cttcatcccg caagtcctga
    12301 agtccggcga ggaaacggga ggcaagctcc tcttccctgg cgcgcacggc ctctacggtt
    12361 tgttccagaa caaaggaaac acccgcaccc aaaccggcga ttccgatcgt gttgggcgtg
    12421 ccgctctcat accggtccgg cataaattcc ggctgctcct caaattcgga gcggctgccc
    12481 gttccgccca tcatcagcgg cgcgattctt tgatccagcc cctcgcggat atagagcccg
    12541 cccgtgccct gcgggccgaa cagagattta tgcccggtaa acgccagcag atcgatgttc
    12601 attttttcaa catcgatcgg caaagcgccg gccgtctggg cggcatcgac gcaaaacatc
    12661 acgtccggtc tgtcctgcag gagctcgcct atggcctcaa ccggcagaat cgtccccgtc
    12721 acattggatg cgtgcgtcac cacgactatt cgggtgtttt tccgcaggac tttttttaga
    12781 tcttccgggt ccatttcccc gcggggcgaa caggggacga ccgacaattc gacgccgcgc
    12841 ccttcaagaa accgcagcgg acgcatgacg gaattatgct ccatgccgga cgtgattacg
    12901 tgatcgcccg gccgaagccc tccctgcaag gcgatgttga gcgcttccgt ggagtttttc
    12961 gtcaaaacga tgcgcagcgg gtcttcaaca tggaaaagaa gcgccaggct ttcgcgcgcc
    13021 tcgcaaataa tgcggccggc gtcaattgaa agcccgtggc cggagcgtcc cggattggcg
    13081 ccgacgttcc gctgataggc cgtcatcgcc gacagcatcg cttccggttt cggaaacgat
    13141 gttgcggcgt tgtccagata aatggttttc attaaacttt ccgtcagacg gtgttttgat
    13201 tacttggccg cgcggatgat gatgtccgaa ccatcctgcc gaatatccgc cttcaagccg
    13261 cgccggctga gaagacggga aacgttttcc cgcgcgactt cgttgtccac cagaatttcc
    13321 aaatctttat tgccgtctgc aatcgcccgg tcaaccagaa ccaccggctg cgggcaggac
    13381 agccctcgag cgtcaacttt ttttgtactc atatcttttc tccttcctga aaatcaagcg
    13441 gatactttat ctcgctgcgt aaagccgaca accagacaga acaaaaaccc gatcaggacg
    13501 gcggccggcc caaaggcggc gatgcctttg ggggatgagg cgatcgcaaa gttatgggca
    13561 attgccgctc ccacgatcat gcccgttgca aaaattgcag cgtccatgtc gccttcgccg
    13621 gacaggaaga gctggcgtcc ggggcagccg ccggccagaa caaacgacag tcctgccagc
    13681 gtcatgccga ggaagttcca gaggtgatcg gtgtgggcga cgggctggcc ttcaaaacca
    13741 ggcttgaatt gccccagaag catattggct ataaacgcaa aaaccagaag cgccgccacg
    13801 ccgctgatga gatgaaagtc ccgaatcaga attacatcgc gaaacgcccc catggtgcaa
    13861 aaccggctgc gctgcgccag gccgccgatc acgagcccgg cgatcaggct gatgaggagg
    13921 ggagcatgct gcgagcccgg cccttttgcg ctgaagaaaa tcggaccgcc cggcgcaaag
    13981 gaaacctgaa acaccaacag cagcaacaaa ccgatcatca aagccggaaa tacccagccg
    14041 gccgctttct tttgcgcata cgacctcccc aggctgtagc cgtttttcag aaacccaacg
    14101 ccgataaaaa tgccggcaat cagccccgcg agccccaaaa tggcgttgcc gtctccgccg
    14161 gccagcctaa ggagtgttcg ccagggacag cccagaaata ccagagcgcc aatcatcgcg
    14221 aagacgccga gaaagaaacg aatcagaggc gaagaaccgc cgcgaggctt atactcaccg
    14281 gcaagaaggg cggcgacaaa agcgcccaga acaaatccca tgatttccgg tcgcagatat
    14341 tgaacgacat cagcgcgatg caatcccagg gcgccggcaa tatcacgctc aaaacaggcg
    14401 acgcaaatcc ccatattggg cgggttcccg aaatactgca gaagggccgc gccgattcct
    14461 attaccgctc ccactagaat gatgccccag cgggttgaaa aaatattttt catttcgatg
    14521 cctcctttat aagatgcgga atttctagca gaagcgccga tcattatcca attcataatg
    14581 ggaatattat catgattgtt ataactgaat ggaatgtgct acaaaacaaa gcaaaagaat
    14641 gcgggtccgc tctttgatcc agtaagggtc gttttgaatt tgtccactcg ctgcctgccg
    14701 cctgcgccat attgcaaagg cagcaacaga aaggcgaacg attgtttcgg ccgaaagcgt
    14761 ctttaccgat tatggcgtta atttgggtta atcatatttc aataagcttc ggcgggccgc
    14821 tcctgctgga cggggcttcg ctgcaaatcg aagccgggga gcgcatcggc cttctggggc
    14881 gaaacggttc ggggaaatca accctgatga aactgctctc cggcgagatt ccgccggatg
    14941 acggtgaaat ttcccgcgat aaaaatattc atgtttccgt catgcctcag gaggtgcccg
    15001 atcttcgcgg aaccgtctat gacgttgtag ccggtggctg tcaggaacat cttgatttac
    15061 ttcatgaata tcatgacttg accctgcggc tgcattcagg cggcgatcat cagcttttga
    15121 aaaaaatcga acaggtccag caccggttgg aagcggtcgg cgcctggcag tatcatcaaa
    15181 aggtggaaga agtgatttct cgggtaagcc tcgatgaaag ttccgaattt cttctgcttt
    15241 cggccgggtt gaagcgccgg gtgcttctgg cgcgggcgct tgttgccgat cccgacgtgc
    15301 tgctgctgga tgaacccacc aaccatctgg atattgatgc catcctctgg ctggaagaat
    15361 ttttaaaaag ttttgagaag acgctgatgt tcgtcaccca tgaccgggcg ttcctgcaaa
    15421 aaatcgccac gcgcattgtg gaaatcgatc ggggcaagct tttttcattt tcctgcgact
    15481 atgcgaccta tctggaacgc cgtcaggcca tgcaggaagc cgaggaaaag cagtgggaaa
    15541 cgttcgataa aaaacttgcc agggaagagg tctgggtccg gcagggcatc aaggcaaggc
    15601 gcacgcgcaa cgaaggccgt gtacgtgtgc tgctggtgct gcgcgaagag cgggcgcaaa
    15661 ggcgcgaacg cgaaggaagc gtccgtctgt ccgtctcgga ggcccaggcg agcgggaaac
    15721 tggtcgtgga agccgagaaa atcagttttg cctatgagaa taaaaagatt atcgattctt
    15781 tttccaccag aattttgcga ggcgaccgtg tggggatcat cgggcccaac ggttccggca
    15841 agaccacgct gctcaaggtg cttttaggag agctgcccgc gaccggcgga aagatccggc
    15901 ttggcgccaa tattgcgatg gcctactttg atcaactgcg cgcgcagttt gatgaaacaa
    15961 aatcgttgcg ggacaacatt gcggatggaa acgacacggt tttcatcaac ggtgttccgc
    16021 gccatgtggt cggttatttg caggattttt tgtttccgcc cgcgcagatt ctcgctccgg
    16081 tggggtctct ttccggcggc gagaaaaacc ggcttctttt ggcgaagctc ttttgcacac
    16141 cttcgaatgt cctgatattg gacgaaccca ccaatgatct ggacgcggaa actttggagc
    16201 ttctggaaga tcgcctgctg gaatacaaag ggacgatcct gctggtcagc catgaccggg
    16261 tgtttctcaa caatgttgtt acctccacca ttgtcctgga aggggaaggg cgtctgcagg
    16321 aatatgttgg cggctatgac gactggctca ggcagcgcgg cgaagcaaag gaaaaaccgg
    16381 cagcggcaaa agaagtaaag accaaaaaag aaaagcagca gagagagaag acaaaattgt
    16441 ctttcaagga aacaaaagaa ctggaagaac tgccgcccag aatcggaaag atggaaaaag
    16501 aaaaggcgga acttttggaa ttgttgaatt ctccggattt gtataaaacc cataatcccg
    16561 cccgcgcgct ggatgtgaac aaccggctgg cagagctgga gacggatttg accgcggcct
    16621 acgaccgctg ggacgcattg gaagaactgg cggcaaagtt ttcagcttag agggtatgaa
    16681 atgatcatcc ggttgatgat ttcttcttta ttgattagaa gattgaaagc tatataattt
    16741 taccttacga ttaagacagt aggacaaccc atatgaaata ttcatataag accgcatgca
    16801 tcatttcatt ttctgtcatc atcttaacat ttacctcgat ctctgctttt ggctctgagg
    16861 cttcaggcct gttcaatatc ggcaatatga gtcatgatat tgtgatgtta atagcctatg
    16921 ttcttctggc tttgttgttt tcatttctat gttcgatagc ggaagctgtt ttattaagta
    16981 ttacgccttc atatatcgag gaacagaaag aaaaaaatcc caagcgggcg gctcacttga
    17041 agcgactgaa acaggataat gtggatcgat cgcttgccgc aattttaaca ttgaacacga
    17101 tcgcacatac tgttggcgca atcggggcgg gtgccaaggc tacagtcgtg tttggaagcg
    17161 catggtttgg agtcttctcg gcggttatga cattgatgat actttttctt tcagaaatcg
    17221 ttcccaaaac gattggtgcg atttactggc caaagctcgt tggccctacg gttattttcg
    17281 tgcgaaccct catcgtgacg ctgttcccga tcgtctggat ttcggaaagg atgacaaaat
    17341 ttatttcaca cggaaagaat ctgcacattt tcagcagaga cgagtttgtc gccatggcaa
    17401 ggataggcga acaatccgga catatagatg ataacgaggc gcggattatc aagaatctct
    17461 tccggttggg gtcaatcaat gtaacagaca tcatgactcc gcgtattgtg attacagcgc
    17521 tttccgatga taaatcggta gcggacgctt cggaatatat tgcaaaaata ccattttcgc
    17581 gaatcccgat atatgagaag gatatagaca atataaccgg ctttgttctg agagatgatg
    17641 tgcttctgcg ggataaccag gaaagaggga atgaattact caagacatta cgaagagaaa
    17701 tactgattgt tccggagtcg gtggtgctta caggtttact ggaaaatttt cttaaaggac
    17761 gtcagcacat cgccattgtc gttgatgaat atggcggaac aatgggtctt gtcacactcg
    17821 aagatttagt tgaaacattg atgggtttag aaattgtaga tgaaatggac acggttgtag
    17881 atatgcgtgt tcttgccaga aaaaaatggg aagaacgcgc aagagcgctt ggcatcaata
    17941 gtgatatcga cgaaagagac ccattcgaaa aattaaatgt tctgccaaac gattcagcga
    18001 agtctgatac ccgggagtga tgatcgttgg tgcgtatcgt atagtaatcg gtaagagaaa
    18061 aggacgcatc aaccggatga aattgaaatg aatctaaaaa acgtaaactt tccccggcag
    18121 cttgaagcgg caggcctcgc ggaaaaaatc cgtctggctt tagcggagcg tcaaacaaaa
    18181 ctctggccgg aaggaaaaat ggaagtgccc gtgttgagca tcagtgaatc cctgatcgcg
    18241 acgatcagaa atgcccgcct gcaaagacgc atccgcttcg ggtttgatga tattttagac
    18301 aagcttgccg ccgagaaaaa aggcatcgac gaaatgctgc aaaagaataa atcccagcaa
    18361 aataacaggg tttcgcgtct acttttgttt tccaacgacg gggcggaacg cctctatcgc
    18421 catatcgagc aggctttagt cgagcaccgt ctccgcattc tgggctgcca gttggacatc
    18481 gacggcaaag agttggggcg gttgattgcc ggaagaagcg ccggcatcaa ggtgattctg
    18541 gtggagcaca aggacgcggt ctccgatgtc ctgcgggcgc ttgcggaagg aaggcaatga
    18601 ccggaaaaga ttggatcctg atttgccggt tgcataatca aggtcttttg ggtataggcc
    18661 atctgcaagg tttcaacgga tcaaaaacag taaactggtc aaggcaagtt catgtccaat
    18721 cgaattgaag tcggttttag ggaaggcatt cgcgacgcgc tgggcgaaaa gacgaaaaaa
    18781 cgcatcatcg acaacctggg actgccggtt gcaggcgccg cgaccatcga agtgtatacc
    18841 attgaaggcg aactgacggc gggtgagctt cgccgcgccg ccgccgggcc tctttccgac
    18901 ccggtgattc aggcgtatgc gatcaataag ccgcttgcca aaaatttcga ctggctcatt
    18961 gaagtgggct ttcgtcccgg cgtcaccgac aatgtcggca aaaccgcgcg cgaagcgatc
    19021 acgcttttgc tgggcgatag cctcggtgaa agaaaaatca gcgtctacac ctcccgccag
    19081 tatctggtct ccggcccgat ttccagggcc gatgcgcaaa acattgcctc cggcctgctc
    19141 gccaacgacc tcatcgagcg ccaccaggtg gtcggcaaag cggagtttga ttttacaggc
    19201 ggcatgcccg tgcatattcc gcaggtcacg ggcagggacg agccgcgggt ggagacgatc
    19261 aatctgacaa agctcgccgc cgcggcgctt ttgaaaatca gccaggaaaa gctccttgct
    19321 ctcaatcttg aagagatgca ggcgatcgcc cggtactttg ccgatccttt gatcgccaaa
    19381 gcccgtggaa aattcggcct ggacgaaaac acaaccgacg tggagctgga gtgcctcgcg
    19441 cagacctggt cggagcactg caagcataaa atctttaaca gccgcatcga gtacaacgac
    19501 gggcgcacga aaaaaatcat caactcgctt tttaaaacct atatcaaagg ctcgacggcg
    19561 aaaatccgca aagccaaggg gaagaaggac ttttgcctgt cggtgttcgt cgacaacgcg
    19621 ggcgtcatca agttcaacaa agaccacaat ctggtcttta aagtggagac gcacaactct
    19681 ccctccgcac tcgatcccta cggcggggcg ctcaccggca ttgtcggcgt caaccgcgat
    19741 ccgttcggca cgggcctcgg cgcaaaactc attttcaaca cggatgtgtt ttgctttgcc
    19801 tcgccctttc acaacaagaa actgccgccc cgcatcctgc accctaggcg catttacgag
    19861 ggcgtgcgcg aaggcgtgga gcacggcggc aacaaaagcg gcattcccac ggtgaacggc
    19921 agcatcgtct ttgacgagcg ctacttgggc aagcccctgg tgtattgcgg cacagccggc
    19981 atcatgcccg cccggcaaaa cggcaaaccg acgcacggca aggaaatcaa atcaggagac
    20041 gtcatcatca tgacgggcgg ccggatcggc aaggacggca ttcacggcgc gacgttttca
    20101 tcggaagagc tgcacgaagg ctcgccggta acggccgtgc agattggcga cccgatcacg
    20161 caaaagaaaa tgacggactt tctgctcaaa gcccgcgatc gggggctgta ccgtgcgctc
    20221 acggacaacg gcgcgggcgg gctgtcgtcc tccatcggcg agatggcgac ttactccggc
    20281 ggctgtgaaa ttgacctttc gctggctccc ttgaaatacg cgggcttgca gccctgggaa
    20341 attctggtgt cggaggcgca ggagcgcatg tcgctggccg tcgattcgaa aaaagtcggc
    20401 aaattcatgg atctggccgc gaagatgggt gtggaagcca cggtgctggg ccggtttacg
    20461 aattccggaa agtttcacgt gcgctacggc gaaaagaccg tggcgtatct cgacatggat
    20521 tttctgcacg acggcgttcc gcagatgaag cttcaggccc gctggaaagc gccgaagcat
    20581 cccgaaccca ggttttccgc accggccgat ttagggaagg ccctggcaca gatgctggct
    20641 cgtttaaata tctgttcgaa agaatccgtt gtacgtcagt acgatcatga agttcagggc
    20701 ggttccgtgg tgaaaccgct ggtgggcgcg caaaacgacg ggccgtcgga tgcgggcgtc
    20761 gtccggcccg tgctcgattc catggaaggc gtggtcgtgg cgcacggcat ctgcccgcgc
    20821 ctgagcgaca tcgacgccta tcacatgacc gcctgcgcga tcgacgaagc ggtgagaaac
    20881 gccgtcgccg tgggagtgga tattgaccat ctggcgggcc tggacaattt ctgctggtgc
    20941 gatcccgtga aatccgccaa gacgcccgac ggggattaca agctcgcgca gctcgtccgc
    21001 tcgaac
//
LOCUS       DAOSIM010000012        20481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_2854_length_20481_cov_8.401841, whole genome shotgun sequence.
ACCESSION   DAOSIM010000012 DAOSIM010000000
VERSION     DAOSIM010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 20481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20481
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2854_length_20481_cov_8.401841"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PLR47_01185"
     CDS             complement(<1..564)
                     /locus_tag="PLR47_01185"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HOG11576.1"
                     /translation="MPAGREDHLEKFKKRYPEKFVPEKDLFGRIRRGNSIFIGTACGE
                     PQSLLNSLIRYVENTPHTIYGTQIIHIWTLGVAPYANKKFKINFRHNSFFISDSSRQL
                     VNEGLADYTPTFLSEIPSLFRRGIITIDVALVQLSPPDEEGLMSLGVSVDIVKSAVEQ
                     ARLVIAQVNHHMPRVRGDAMVDIRDVDF"
     gene            901..1278
                     /locus_tag="PLR47_01190"
     CDS             901..1278
                     /locus_tag="PLR47_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOG11577.1"
                     /translation="MFQIVKYETFNDRQVIFKEGTFGDWMYVIEEGAVEISRVVEGKK
                     IVIAVLKTGEIIGEVAYISKEGRSATATAVGKTRIGMIDREYFDREFNGISSDFQMIL
                     KSMARRLRETTDHLIQLQNQAGK"
     gene            complement(1296..2237)
                     /gene="fmt"
                     /locus_tag="PLR47_01195"
     CDS             complement(1296..2237)
                     /gene="fmt"
                     /locus_tag="PLR47_01195"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOG11578.1"
                     /translation="MTKKPRILFMGTPAFALPALETLCQRGFPISAVVTQPDRPAGRG
                     KKDAAPPVKQMAQKFGLTVLQPASVKDPSFLEALDAVRPDMAVVAAFGQILPKAVLDF
                     PPQGCLNIHPSLLPAYRGAAPIHWSIIRGEKQTGVTIMLMDEGMDSGDILAQEKTPVG
                     ETETYGELHDRLALLGAALLVETIERLASGAVQRQKQDSSRVTFAPRLSRETGRICWR
                     NPAEHIINLVRGLNPSPAAYTLLEGQDLKILAAEARPGPVEEPPGAVAAATPEGLPVA
                     ASNGHVILKEVQLAGKKRMPVSDFLRGFPLKPGIRLE"
     gene            2415..2873
                     /locus_tag="PLR47_01200"
     CDS             2415..2873
                     /locus_tag="PLR47_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11579.1"
                     /translation="MKTYAIKYVELAEQHAGRMAKRWAMDVQNNARTKRYKELDEESI
                     VNQGVKFYHNFSKMFAEDKISEATMKYFRTYAQESFAMRIPMDEAVYALILLRRHTWL
                     YAEFQTIFSSGIDQRQALDTLSRTILLFDYAIYEVTKEYQELMKKGKGKK"
     gene            2921..3379
                     /locus_tag="PLR47_01205"
     CDS             2921..3379
                     /locus_tag="PLR47_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11580.1"
                     /translation="MVESIKLLDVLEANIDEIAVHWAADVRKNKRTVHYKDIPEEKLV
                     LQAVKFYSQLRNMLMAPNRLDKAQEFFMHYARTCYEMGIPLHESIYALNMMRRHMWLY
                     AEFQAIFINAIEHNQAIDSVMRIMLLIDYAVYEITQYYQDRIRLETKQQF"
     gene            complement(3458..5866)
                     /gene="priA"
                     /locus_tag="PLR47_01210"
     CDS             complement(3458..5866)
                     /gene="priA"
                     /locus_tag="PLR47_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOG11581.1"
                     /translation="MFISVAVNIPSDKLFTYEVPVHLQNAVEVGKRVFVPFGIRKRSG
                     FIVEILKNCDLKDTKKIVAVLDEEALFDAEDLKFYRWISGYFLYPLGKTLAELIPSGP
                     ERRELRWIIPAPAARDARPTAAQKKLLDILHEHSGGLAFQDWIRKSNMKNAASIARSL
                     QVAGWVHVMEKEKKQLGIRMKKIIRLADELSGPMKLTPRQQIVADCLRREGPMELRAL
                     AEKTAASAAVIKRLFEKGAVEVREEEHIRRAPIESALAAPRQNIALNDEQKKALETIG
                     QFIDSEVFHPVLLHGVTGSGKTEVYLCAIDRVLKSGGSAIYLVPEISLTPQLISRITG
                     RFDPDRIAVLHSGIAESVRYDQWRQIRRGLIHLVIGARSALFAPLPNLKIIIVDEEHD
                     PSYKQDERLCYNARDLAVVKARSANAVCVLGSATPAVRTFFNARGGKYAHLELARRVN
                     RQPLPQIDIVDMKTQRETGGKSPVLSEALIAALRNVLDRREQALLFLNKRGFDTFLVC
                     ADCGYTFSCPNCAVSLKSHPAENLVKCHYCDYSRRAVPLCPRCGGSRILNYGAGTQKL
                     EAELQSLFPLARISRMDSDTTARRGSQEKILASLERGEIDILVGTQMVSKGHDFPSIT
                     LVGVVSADTSLNMPDFRAAEKTFQMLTQVAGRGGRGEATGRVIIQTFNPLHYALRHAS
                     AHDYLSFYEEEIEFRKALKYPPFAQIINLRLSSARKAALDETARELGRDARALCAGHE
                     NILEIIGPAESPLAKIRGEHRQQMMIKGRDNRRMHSMAAKLLKKHATSTVKITADVDP
                     ENFM"
     gene            complement(5955..6209)
                     /locus_tag="PLR47_01215"
     CDS             complement(5955..6209)
                     /locus_tag="PLR47_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11582.1"
                     /translation="MEKQTTLKPEKLEAFRKLLTQKINELLSEAGKTVSEMTSGKENF
                     PDPNDRASLESDRNFELRIRDRERKLCKTKQEKMEKLKGE"
     gene            complement(6343..6828)
                     /gene="rfaE2"
                     /locus_tag="PLR47_01220"
     CDS             complement(6343..6828)
                     /gene="rfaE2"
                     /locus_tag="PLR47_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOG11583.1"
                     /translation="MNKILNRTELKEKLDALRQEGKKIAFTNGCFDLLHVGHVRYLKE
                     AGKTADVLVLALNSDSSVRAIKGLKRPLVPEQERAEILAELECIDFITIFSEPTPLEL
                     ICLLKPDVLIKGGDWAEEQVVGRDEIRRWGGRVEIIAEVAGKSTTNIVDKVMAVYGPD
                     K"
     gene            complement(6987..7715)
                     /locus_tag="PLR47_01225"
     CDS             complement(6987..7715)
                     /locus_tag="PLR47_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOG11584.1"
                     /translation="MILYEFLSQISDAFFPRICLACSRILPSGRPEFFCSDCFGQITF
                     LHGSLCPVCGILFPDSSSEDHLCPSCMENAHWFEFARAAVAYEGIIADVIRRFKYGRD
                     LTAGEALAGLLADFDFDGVDFSAFDAIIPVPLHIRRLRERRFNQSLVLARALGKKHGI
                     AVDFSLLKRRRFTLTQTGLDKSERDKNISGAFRASHSEKIPGGSFLLVDDVYTTGATI
                     NECAKTLAEAGAKRVAALTAARVL"
     gene            complement(7712..8107)
                     /gene="rsfS"
                     /locus_tag="PLR47_01230"
     CDS             complement(7712..8107)
                     /gene="rsfS"
                     /locus_tag="PLR47_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOG11585.1"
                     /translation="MPVKKNESARRLLLCINAVLQKKAKNIVVLNVREISSFTDYMLI
                     CSGTTDRQVQAVASTVQEYLKKEGIRPLGVEGKANAEWILLDYDDVVISIFQEAARSF
                     YDLENLWDAPRMEIDENATEIKKLHKDMT"
     gene            8488..9285
                     /locus_tag="PLR47_01235"
     CDS             8488..9285
                     /locus_tag="PLR47_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOG11586.1"
                     /translation="MPDMIRKVHVHMPYLLLGRHLPMVLAKKLNLEIYFSHDALQSLD
                     RNQCAKVSRTLENAGLKVTFHAPFMDLRPGALDDGIRRASLDRIRQVFDLIPIFKPLK
                     IVCHPSFDQRYYVSCDDLWLENSVNFWRQLIVQAEDGGAVIALENVYEREPGILRRLL
                     EALHSRQVCFCFDTGHFNVFARAPLEDWMEQLGRFIGHLHLHDNFGKFDEHLPVGGAT
                     FPFGLFFAALKKLDVRSTVTLEAHAEEHLWQSLANLRNSSYLDGLNL"
     gene            9300..10274
                     /locus_tag="PLR47_01240"
     CDS             9300..10274
                     /locus_tag="PLR47_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG11587.1"
                     /translation="MFPYLVKRILFMIPLLLGITIICFFVMRLAPGSPTDLQTQMNPK
                     ASAEMRQRLMSLYELDKPIHVQYVSWLTKLIRGDLGTSFSSDHRPVADKILERLPITI
                     TINLLSLVLIIAVAVPIGVLSAVHQDSLFDKVMSVIVFIGFAVPTFWLALLMMIFFGI
                     HLGWLPISGLRSLNYEYLSTWGQWTDLAKHLILPVFISAFGGLAGLSRYMRANMLEVI
                     RQDYILTARAKGLSERQVIYKHALRNALLPAITILGLSIPGLIGGSVIFETIFAIPGM
                     GQLFYMSVMARDYPTVMGILLIGAVLTLAGNLIADVSYAAADPRIRIS"
     gene            10299..11111
                     /locus_tag="PLR47_01245"
     CDS             10299..11111
                     /locus_tag="PLR47_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG11588.1"
                     /translation="MFYKNKLALAGLAIVVLLFVISLAAPLISPCDPGAIDLQNVLEP
                     PSVEHWFGTDQLGRDVLSRMIWGARISLKVGFVATGLAILIGMVLGAVAGYYGGWVDA
                     VIMRFVDVMLCFPAFFLILAVIAFLEPSIWNIMIVIGLTGWMGVTRLVRADFISLRER
                     DFVWAARAIGANDARIIFLHILPNALASILVAATLGIAGAILTESALSFLGIGVQPPT
                     PSWGNILTAGKDNIDIAWWLSLYPGLAILITVVGYNLLGEGIRDASDPRLKR"
     gene            11234..11410
                     /locus_tag="PLR47_01250"
     CDS             11234..11410
                     /locus_tag="PLR47_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11589.1"
                     /translation="MSKRVNPELLKIDRRIIARKLLIGELSEKDLSSLLKKLPDVSEN
                     AEEIQIQPGEKPSC"
     gene            11404..12180
                     /locus_tag="PLR47_01255"
     CDS             11404..12180
                     /locus_tag="PLR47_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOG11590.1"
                     /translation="MLIDSHAHLEMKEFDPDRSDVIERARAAGVDCIVTVGTNPALSR
                     KALSIARRHENIYSTVGIHPHDAAAADDSSLDELKKLAQDPKVVAYGEIGLDYFRDLS
                     PREKQAEMFARQLDLAGELRLPVIIHDREAHEQTLRMVKASGIRSGVFHCFSGDWSMA
                     RQCLDLGFYLSVPGVVTFDRSKVLQEVVRQAPMDWLLLETDCPYLAPVPHRGKRNEPS
                     FLVHTARKVAQIKGLAWEDVAQTAARNACRLFCIPPPALK"
     gene            12220..12942
                     /gene="queC"
                     /locus_tag="PLR47_01260"
     CDS             12220..12942
                     /gene="queC"
                     /locus_tag="PLR47_01260"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOG11591.1"
                     /translation="MNDPSIKTRPRAVVLLSGGVDSATTLAIARNMKFDVHALTFRYG
                     QRHMVELEAAARVARENGVVEHRTVDIDLRCIGGSALTADIAVPKHGSVEELGKNIPM
                     TYVPARNTIFLSYALAWAEVIGSSNIFIGVNALDYSGYPDCRPEYIEAYERLANLATR
                     AGVEGTEKLRIHAPLIHWSKAQIVKRGMELGVDYSLTHSCYDPDADGASCGRCDSCLL
                     RLKGFKEAGIKDPGRYRLKTEG"
     gene            12993..13631
                     /gene="queE"
                     /locus_tag="PLR47_01265"
     CDS             12993..13631
                     /gene="queE"
                     /locus_tag="PLR47_01265"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR04508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HOG11592.1"
                     /translation="MYSIKEIFYSIQGEGYHTGRPAVFCRFAGCNLWSGREEDRSRSI
                     CKFCDTDFVGTDGINGGVYRSSAELAHRISLAWPVKKNKLTRPFVVCTGGEPLLQLDA
                     ALISALHKENFEIAVETNGTLPAPAGLDWICVSPKADAPLKQAAGDELKLVFPQEGAE
                     PKKYERLAFQHFFLQPMDSPHGGEAVRLSLAYVLAHPRWRISLQMHKVLGVR"
     gene            13634..14014
                     /gene="queD"
                     /locus_tag="PLR47_01270"
     CDS             13634..14014
                     /gene="queD"
                     /locus_tag="PLR47_01270"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOG11593.1"
                     /translation="MMEIFKVFHFDAAHSLPRVPEGHKCAKMHGHSFRVEIHLRGKAG
                     SDSGWVTDFADIAAAFEPILEQLDHKNLNAVEGLENPTSENLCRWIWLRLKPVLPQLS
                     RIVLQESPESGCIYEGEDEPEAAI"
     regulatory      14046..14157
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            14218..14889
                     /locus_tag="PLR47_01275"
     CDS             14218..14889
                     /locus_tag="PLR47_01275"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOG11594.1"
                     /translation="MNRCIVIISGGRLGDMDFFRKKMSEAGSDLLIGCDGGARHLAAA
                     GVTPDVLVGDMDSIESSRLADYVRRGVKIIQHPADKDFTDTALALDYALGLKPERIQI
                     WGALGGRIDHALANLFLLVRAKEAGVAACLLDEYCEVFVPVDDAVFENRKGCLVSLLA
                     LSPRVEGVTLEGFAYPLEEGTFELSESRGVSNIIAAERARIRFRSGHLLVIRYRQKDF
                     FPEAD"
     gene            14890..15714
                     /gene="thiD"
                     /locus_tag="PLR47_01280"
     CDS             14890..15714
                     /gene="thiD"
                     /locus_tag="PLR47_01280"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOG11595.1"
                     /translation="MGKPIRILCIGGSDSGGGAGIQADLKAVHACGGYAVCAVTAVTV
                     QNTRGVQSVLPVPPKWIAAQLDSVLEDIGADAVKTGMLMTPEAVETVAGKIKQYRLKN
                     IVVDPVMIAKGGRSLMTDRARRALVKTLLPLADVLTPNIPEAEFLSRMKIRSVEDMKK
                     AAAVLSDLGVKRVVVKGGHLPGRKTRGCVDILYDGLRYDMFAAKWTQTKNTHGTGCTF
                     ASVLASCLAQGRGTQEAVRQAKRMTERAIQHSLSLGRGHGPVNLWGDFGVKKEKKS"
     gene            15711..16982
                     /gene="thiC"
                     /locus_tag="PLR47_01285"
     CDS             15711..16982
                     /gene="thiC"
                     /locus_tag="PLR47_01285"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOG11596.1"
                     /translation="MTQLEKARKGVLTEEMKQSAAREGVSPEAIRQGIVDGTIVICRN
                     INHTAIEPLAIGLGLRTKVNANIGTSRDNQDLKLELEKLSVAIAAGADAVMDLSTGGN
                     LADIRKAVMAKSSVAIGTVPIYQAAVKTLEEKKAISEMTPDDLFGVIEENGRDGVDFI
                     TVHCGVNRESVAAVEAQGRVLGIVSRGGSMTANWMRCNDRENPLYEEYDRLLDIARRY
                     DMVLSLGDGLRPGAIHDATDQGQLMELIILGQLAARARAAGVQVMIEGPGHVPITEVE
                     ANIKLQKKLCQGAPFYVLGPLPTDIAPGYDHITSAIGGAIAGAAGADFLCYVTPAEHL
                     RLPTLADVRDGVMAARIAAHIADIAKGIPGAFERDRKMSECRRKFDWQGQVKASLDPD
                     KTSALLEKSKSANEEGCTMCGEFCAIRLGKK"
     gene            17008..18357
                     /locus_tag="PLR47_01290"
     CDS             17008..18357
                     /locus_tag="PLR47_01290"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOG11597.1"
                     /translation="MIRKADDHSIEKRLGAKLVVEGLRRAGFEAYFVGGCVRDFVRGV
                     SPGDYDIATSAPPDQVIRLFERTVAVGAKFGVVVVCEGGFSYEVATFRSDEAYEDGRR
                     PTQVRFSSAREDVLRRDFTINGLLMDPETNDIVDYVGGREDIGKKIIRTIGDPAVRFS
                     EDYLRMLRAIRFAAHLDFHLEDGVHEAMVRHTAKIREISAERVQEELNKILTRSGARR
                     GLELMADTGLLKHILPEVDRTRGVAQPPRFHPEGDVWRHTLIMMEILSNENRPEAEAA
                     LAWGALLHDVGKPVSRTEDEKGVHFYGHSVTGVSIAEDVMRRLRFSRALRETVLDLIR
                     QHMAFMNVQKMRPARLKRFLRMPNFHLHLQLHRLDCLASHKILDHYAFCVEQLQKTEP
                     EDLHPPRLLTGDDLLAMGFAPGKQMGEILRALEEEQLENRIHEKEEAVAFVQTRWKF"
     gene            18365..19147
                     /locus_tag="PLR47_01295"
     CDS             18365..19147
                     /locus_tag="PLR47_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOG11598.1"
                     /translation="MRAKGKMKPILLRLAVLFMMGLLLQACAGREAKVYPGSHRPQKR
                     QLAEMGYTIQAGAFARVENAVRLTQRLKGQGLPATYFRADDGLFKVRFGNFSTKEPAR
                     LRAESLAQAGVIEEYYIVLPEDSPAAKRKQYGTDYLRESLVKTAHDFIGVPYLWGGVS
                     ADTGFDCSGLAMTVYQLNGLDLPRHSRTQFDAGDSIARQELQKGDLVFFDTRGGGKVS
                     HVGIYIGAGRFIHAPSSGKKIRIDSLASDYYARRFIGAKTYL"
     gene            19396..19566
                     /locus_tag="PLR47_01300"
     CDS             19396..19566
                     /locus_tag="PLR47_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11599.1"
                     /translation="MAATAKKVVKKAAAKKPAAKKKVVKKVAAKKPAAKKKVAAKKPV
                     AKKKVVKKAAKK"
     gene            19689..19883
                     /locus_tag="PLR47_01305"
     CDS             19689..19883
                     /locus_tag="PLR47_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11600.1"
                     /translation="MMLRPCRSPGVKAPVPEVKGAVTGFGDVHRRAHLCRAAGEGPAY
                     AHRKAKERQEKRGGRKRNRR"
     gene            19993..>20481
                     /locus_tag="PLR47_01310"
     CDS             19993..>20481
                     /locus_tag="PLR47_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG11601.1"
                     /translation="MLELQKVYFTVQDTEGSDGGPRNIIDGIDYVFEDGKFYVITGPN
                     GSGKTTLAKLIMGVNPLSGGKILFNGQNISTLTITQRAKEGIAYSFQQPARFKGITFR
                     DLLTIATGIEEEPRLLEILGRVGICPLSFLDKEVDARLSGGEIKKIELATTIAANPKL
                     AIY"
BASE COUNT         4381 a         5415 c         6095 g         4590 t
ORIGIN      
        1 gaaatccacg tcgcggatat ccaccatggc atcgccgcga acgcgcggca tgtgatggtt
       61 gacctgcgcg atgaccagcc gggcctgttc cacggcggat ttgacgatgt ccacgctcac
      121 gcccaggctc atcagccctt cttcgtccgg cggcgagagc tgcaccagcg cgacatcgat
      181 ggtgatgatt ccgcggcgaa agagagacgg gatttccgaa aggaacgtcg gcgtatagtc
      241 ggccagccct tcattgacca gctgccggga gctgtcgctg atgaaaaacg aattgtggcg
      301 aaagttgatc ttgaactttt tgttggcata gggagccacg ccaagggtcc atatgtgaat
      361 gatctgcgtc ccgtaaatgg tgtggggcgt gttttccacg tagcgaatga gcgagttcag
      421 caggctctgc ggttcgccgc aggccgtgcc gatgaagatg gaattgccgc ggcggatgcg
      481 tccgaaaagg tctttttcgg ggacgaattt ttccgggtag cgctttttga atttttcaag
      541 atggtcttcc cgccctgcag gcatggccgt tcctccgttg ccttcccgtc tccagacccc
      601 gctggaccgg ccggcgttgc cttcggccgt tcgtttcgcg cccggaatcg gcctttgccg
      661 ccttgccgaa acacgatgag atcacacgag agcttttctt tgcctcggaa ctatagaaag
      721 tcccgttttg taagtcaaga agaacttgat cccctttccg gtgagggcct cggctggatg
      781 gaaagtgcga atttccgggg gccggcccat tgggattggt ggttgagttc atgggaaatt
      841 catgttagaa aaaatccggt caatgaatcc ggcacaccaa aaggcgaaag ggggcgcccc
      901 atgttccaga ttgtaaaata cgagaccttc aacgaccggc aggtgatctt taaagaagga
      961 acctttggcg actggatgta cgttattgaa gagggggccg tggaaatttc ccgagtcgtc
     1021 gaagggaaaa agatcgtcat cgcggttctg aaaacggggg aaatcatcgg ggaagtggcc
     1081 tacatttcca aagaagggcg ctccgcgacg gcaacggcgg tgggcaaaac ccgaatcgga
     1141 atgatcgacc gggaatactt cgaccgggag tttaatggaa tctccagtga ttttcagatg
     1201 atcctgaaat ccatggcccg gcgcctgagg gaaacaaccg accatctgat acaacttcaa
     1261 aaccaggccg ggaaatagac ggcgcgcgtc gggcctcatt ccagccggat tccgggcttc
     1321 agtggaaagc cgcgcagaaa gtcggaaacc ggcattcttt ttttgccggc cagctggacc
     1381 tccttcaaga tcacatggcc gttcgaggcc gccacgggca gcccctcggg ggtggctgcg
     1441 gcgacggcgc cgggcggttc ttccacaggg cccgggcggg cctcggcagc aagaattttc
     1501 aggtcctgcc cctccaggag cgtgtaagcc gcgggcgacg gattcagacc gcgaaccaga
     1561 ttgatgatgt gttcggccgg attgcgccag cagattctgc cggtttcccg gctcaggcgg
     1621 ggcgcgaagg tcacgcggga agaatcctgc ttttgtcttt gaacggcgcc ggatgcgagc
     1681 ctttcgatcg tttcgaccag cagcgccgcg cccagcagcg cgagccggtc atgcagttca
     1741 ccgtaagtct ccgtttcgcc gacgggcgtt ttctcctgcg ccagaatatc gccggaatcc
     1801 attccttcat ccatcagcat gatggtaacg ccggtttgtt tttcgccccg gatgatgctc
     1861 cagtggatag gcgccgcgcc ccggtaggcg ggcagaaggg aagggtgaat gttcaggcag
     1921 ccctgcggcg ggaagtcaag gacggctttg ggcagaatct gtccgaaggc cgccacgacc
     1981 gccatgtcgg gacggacggc gtccagggcc tccagaaaag acgggtcttt tacgcttgcc
     2041 ggctgcaaaa ccgtgaggcc gaatttttgc gccatttgct tcacgggggg agcggcatcc
     2101 tttttgcccc ttcccgcggg gcggtccggc tgggtgacga cggcggagat tggaaagcct
     2161 cgttggcaga gcgtttccag ggccggcagg gcaaaagccg gagtgcccat gaagagaatg
     2221 cgtggtttct tcgtcatttc ccgtcctttt ctttttcacg cttctatatg gaattgtgaa
     2281 gtttgtgtca agggccgctc ctgaccggcg gttttccggc attttcctgt tgtcaatttc
     2341 ggtttcatgc tttatattga agacaacaaa acgaatctca tgacaggtgg atctttcacg
     2401 aaaggagacg aagcatgaag acatacgcca ttaagtatgt ggagctggcc gagcagcatg
     2461 cgggaagaat ggcaaaacgg tgggcgatgg acgtccagaa caatgcccgg acaaaaagat
     2521 acaaggagct ggacgaagaa agcatcgtga atcagggtgt aaagttttat cacaatttca
     2581 gcaaaatgtt cgccgaagac aaaatcagtg aagccaccat gaaatatttt cgaacctacg
     2641 cacaggaaag tttcgccatg cggattccca tggatgaagc cgtctacgcg ttgattcttc
     2701 tgcgaaggca tacctggctg tacgcggaat tccagaccat cttcagctcc ggcatcgatc
     2761 agcggcaggc cctggacacc ctgagccgga cgatcctgct ttttgactac gcgatttatg
     2821 aagtcacgaa ggaatatcag gaattgatga agaagggaaa aggcaagaaa taatttccgt
     2881 atcccctgca cgcataggat ggaagagagg agaatgcgat atggtagaat cgattaagct
     2941 gctggatgtg ctggaagcca atattgacga aattgcggtg cactgggccg ccgacgtcag
     3001 gaaaaataaa cgaaccgttc actacaagga cattcctgaa gagaagctcg tcctgcaggc
     3061 cgttaaattt tacagccagc ttcggaacat gctcatggcc cccaatcgtc tggacaaggc
     3121 gcaggaattt ttcatgcact acgcgagaac ctgctacgaa atgggcattc cgcttcatga
     3181 atccatctac gccctgaaca tgatgcgccg ccacatgtgg ctctacgcgg agttccaggc
     3241 gattttcatc aacgccatcg agcacaatca ggccatcgac tccgtcatgc ggatcatgct
     3301 gctcatcgat tatgctgtgt atgaaatcac gcagtactat caggaccgca tccggctgga
     3361 gaccaagcag cagttttagc gttcctgggg gcggctggat tcaaacagac ccaaccggcg
     3421 gcagcggatt ttccgctgcc gtttttgccg gcattcctta catgaaattt tccggatcca
     3481 cgtccgccgt gattttgaca gtgctggtgg cgtgcttctt taaaagcttt gcggccatgg
     3541 aatgcatccg ccggttgtcc cttcctttga tcatcatctg ctggcggtgt tctccccgga
     3601 ttttcgccag cggcgattcg gccgggccga tgatttccag aatgttttca tgcccggcgc
     3661 aaagcgcccg ggcgtctcgt cccagctccc gcgccgtttc gtcgagcgcc gcttttctgg
     3721 ccgaggacag ccgcaggttg atgatttgtg cgaacggggg gtatttcagc gccttgcgga
     3781 attcgatttc ctcttcataa aaggataagt agtcgtgggc gctcgcgtgg cgcagcgcgt
     3841 aatgcagggg gttgaaggtc tggatgatga cgcgccccgt tgcttcgcct cttccgccgc
     3901 gtcccgccac ctgcgtcagc atctgaaacg ttttttccgc ggcgcggaaa tccggcatat
     3961 tgagcgacgt gtcggcggaa acgacgccca ccagcgtgat ggacggaaaa tcgtgccctt
     4021 tggagaccat ttgcgttccc accagaatgt cgatttcgcc tcgttccagc gaagccagaa
     4081 tcttttcctg cgatccgcgg cgcgccgtag tgtcggaatc catccggctg atgcgcgcca
     4141 gcgggaaaag gctctgcagt tcagcctcca gtttctgcgt gcctgctccg tagttgagga
     4201 ttcggcttcc gccgcaccgg gggcaaagcg gcacggcgcg gcggctgtaa tcgcagtagt
     4261 ggcatttcac cagattttcc gccggatggc ttttcaggga aacggcgcag ttggggcagg
     4321 aaaacgtgta gccgcagtcg gcgcagacca gaaacgtgtc gaagccgcgc ttgttgagaa
     4381 aaagaagcgc ctgctcccgg cgatcgagaa cgttccgaag cgccgcgatg agcgcttcgg
     4441 aaagaacggg tgatttgccg ccggtttccc tttgcgtttt catgtcgaca atgtcgattt
     4501 gcggcagtgg ctgacggttg acccttcggg ccaactccag gtgagcgtat tttccgccgc
     4561 gggcgttgaa gaaggtgcgc acggccggcg tcgccgagcc gagcacgcag acggcgttag
     4621 cgctccgggc tttgacgacg gccaggtcgc gggcgttgta gcagagccgt tcatcctgct
     4681 tgtacgacgg atcgtgctcc tcatccacga tgatgatttt caggttcggc agcggggcaa
     4741 acagtgccga gcgcgcgccg atgaccagat ggatcaggcc ccgccggatc tggcgccact
     4801 ggtcgtagcg gacgctctcg gcgatgccgc tgtgcaggac ggcgatcctg tccgggtcga
     4861 agcgcccggt gatccgggag atcagctgcg gtgtgaggga aatttccggg accaggtaaa
     4921 tggccgagcc gcccgatttc agaacccggt cgatggcgca caggtacact tccgttttgc
     4981 cgctgccggt cacgccgtgc agcaggacgg ggtggaatac ctcgctgtcg atgaattgcc
     5041 caatggtttc cagggccttt ttttgttcgt cgttgagcgc gatattctgt ctgggggcgg
     5101 cgagagcgga ttcgatcggc gccctgcgga tgtgttcttc ttcgcggacc tctacggcgc
     5161 ctttttcaaa aagccttttg atgacggcgg cacttgccgc ggttttctcc gccagggcgc
     5221 gaagctccat cggcccttcc cgccgaaggc agtcggcgac aatctgctgc cggggcgtca
     5281 gtttcatcgg gccggagagc tcatcagcaa gccggataat ttttttcatg cggatgccca
     5341 gttgcttttt ttccttttcc atgacgtgaa cccaaccggc cacctggagg ctgcgggcga
     5401 tggaagccgc gtttttcata ttggatttcc ggatccaatc ctgaaaggcc aggccgcctg
     5461 aatgctcatg gaggatgtcc agaagctttt tttgcgcggc agtcggcctc gcatcgcggg
     5521 cggcgggtgc gggaatgatc cagcgcagtt cccttctttc cggtcccgag ggaatcagct
     5581 cggccagcgt tttccccagc ggatagagaa aatacccgga aatccaccgg taaaatttca
     5641 gatcctccgc gtcgaacagc gcctcttcgt caaggacggc gacaatcttt ttggtgtcct
     5701 tcaaatcgca gttcttcagt atttcgacaa taaatccgct gcgctttctg atgccgaagg
     5761 gcacaaaaac cctcttgccg acctcaacgg cgttctggag atggaccgga acttcatagg
     5821 taaagagctt atcggaagga atgttgacgg cgacgcttat gaacatgagt cttgttacca
     5881 cttttgacgc aaaaagaaaa tatcggacgg gaaacgggcc aaaaaaaagc cccgactcgc
     5941 ggggcttccg gtctttattc gcctttgagc ttttccattt tttcctgctt ggttttgcag
     6001 agtttgcgct cgcggtcgcg gatgcgcagc tcaaaattgc ggtcggattc cagggaggcg
     6061 cggtcgttgg gatcggggaa attttccttg ccgctggtca tttccgaaac ggttttgccc
     6121 gcctcgctga gcaattcatt gattttttgt gtcagcagtt tgcgaaatgc ttcgagcttt
     6181 tccggcttta aagtcgtttg tttttccaat gccgccactc ctttaccgta atgtttgaag
     6241 ggacctttta cctaatggcg actggtttgt aaagcaaaaa aatcatgtat aaaaatccga
     6301 aaaaaatcca taatcccgca tatccacccg cctgttccgc cttcatttgt ccggtccgta
     6361 caccgccatg accttgtcca cgatattggt cgtggatttg ccggcaactt ccgcaataat
     6421 ctcgacccgt ccgccccacc gccggatttc atcgcgtccg acgacctgct cttccgccca
     6481 gtccccgcct ttgatcaaaa catcgggctt gagcaggcaa atcagctcca gcggtgtcgg
     6541 ctcggaaaat attgttataa aatcgatgca ttccagctcg gcaaggattt cggccctctc
     6601 ctgctcggga accagcggtc ttttgagtcc cttgatggcc cgcacggagg aatcgctgtt
     6661 gagcgccaga accagaacat cagccgtttt tccggcttcc ttcaggtatc ggacatggcc
     6721 cacgtgaaga agatcaaagc aaccgttggt gaaggcgatc tttttcccct cttggcgcag
     6781 cgcatccagt ttttccttta attcggttct gtttagaatt ttgttcatag cgactccttt
     6841 tgattcgtcg gcaacccggg cttttttatt gaaaccgttt ggccgtcccg gccaagcaaa
     6901 cgttttgacg cattttccga agacgatccc gatggttccg ttggtgatcg gctgtccctt
     6961 cgggctgccg tgaggccggc cggcgtttaa aggacacggg ccgccgtcag cgccgccact
     7021 cgtttcgcgc ccgcctccgc cagcgttttg gcgcattcat tgatcgtggc tcccgtcgtg
     7081 taaacatcat ccaccagcag gaaactcccg ccgggaatct tttctgaatg acttgccctg
     7141 aaagcgccgg aaatgttttt atcccgctcg cttttgtcca gtcctgtttg cgttaaagta
     7201 aatctgcgcc gttttagcaa ggaaaaatcg acggcgattc cgtgtttttt gcccagagcg
     7261 cgcgccagga caagcgactg gttgaatcgt ctttcccgca gccgcctgat gtgcagcggg
     7321 acgggaatga tcgcgtcaaa agcgctgaaa tcgacgccgt caaaatcaaa gtcggccaga
     7381 aggcccgcca gcgcctcgcc ggccgtcagg tcccgtccgt acttgaagcg gcggatcaca
     7441 tctgcgatga ttccctcata ggcaacggcg gcgcgggcga actcgaacca gtgtgcgttt
     7501 tccatgcagg aagggcacag gtggtcttcg gaagatgagt cgggaaacag gattccgcag
     7561 accgggcaga ggcttccgtg aagaaaggtg atttgaccaa agcagtcgga acagaagaac
     7621 tccgggcggc cggagggaag gatgcgggag caggcaaggc agattctggg gaaaaaagca
     7681 tcggagatct gggataaaaa ctcataaaga atcaagtcat gtccttgtgc aatttcttga
     7741 tttccgttgc gttttcgtct atctccattc ggggcgcgtc ccacaggttt tcaagatcgt
     7801 aaaaagaacg ggcggcttcc tgaaaaatgg agatcaccac atcgtcataa tccagcagga
     7861 tccattcggc gttggccttt ccttccacac ccagcggccg gatgccctct tttttcagat
     7921 attcctgaac cgtggaggca acggcctgaa cctgccggtc ggttgttccc gagcagataa
     7981 gcatgtagtc cgtaaaggaa gaaatctccc gaacattcaa aacaacaata ttttttgcct
     8041 ttttctgcag aaccgcgttg atgcacaaca gcagccgcct ggccgattca ttttttttaa
     8101 ctggcaaaat tcctccttga ataggttttt aggctgaccc cctgcaaggt ggcacgatgc
     8161 cgacacccct gcgggcgcgc gcggctttaa ggataaaatg aaagacgcta aacgcgccgt
     8221 cattcagaaa acttataacg ccaggagtgc gcaaaatcaa ccttcagtct tcagccttca
     8281 atcttaattt tcttatgtca caaatgcaaa aagttgacaa taaggcattt tggtccctgc
     8341 cgggtgatca cggccgtttc cctgaatgca gggagctttt gcctccttca tcaatgtcgc
     8401 agaaggaaga gcagggatgg gaggttgtga aaaaaaaacg actgtggtaa atcaacggtc
     8461 cctgtcgatg aagaaaggaa tctctgaatg cccgacatga ttcggaaagt tcatgttcac
     8521 atgccgtatc tgctgctggg gcggcatctg cccatggttc tggcaaagaa gctcaatctg
     8581 gagatctact tcagccacga tgcgcttcag tcgctggatc ggaatcagtg cgccaaggtg
     8641 tcccgaacgc tggagaatgc cgggttaaaa gtgacgtttc atgccccttt tatggatttg
     8701 cggcccggcg cgctggacga cggaatccgc cgggccagcc tggacaggat tcggcaggtg
     8761 ttcgatctga tcccgatttt taagccgctg aaaattgtct gtcatccctc ctttgatcaa
     8821 cgctactatg tgtcctgcga tgatctttgg ctggaaaaca gcgtcaattt ctggcggcag
     8881 ctgattgtgc aggccgaaga cggaggagcc gtcattgcgc tggaaaacgt ttacgaaagg
     8941 gagccgggca tcctgcgccg cctgctggaa gcgctccatt cccgtcaggt gtgtttttgt
     9001 ttcgacacgg gccacttcaa tgtttttgcc cgcgcgcccc tggaggactg gatggagcag
     9061 ctgggccgct tcatcggcca tctgcatctt catgataatt tcggcaaatt cgatgagcat
     9121 ctgccggtgg gaggcgccac ctttcctttc ggcctgtttt ttgccgcgct gaagaagctg
     9181 gacgtgaggt cgacggttac gctggaggcg catgccgagg agcacctctg gcagtctctg
     9241 gcgaatttgc gcaacagttc ctatctggac ggtttgaacc tctgaggggg gagtggggga
     9301 tgttcccgta cctggtcaag agaattcttt ttatgatccc gctgctcctg ggcatcacga
     9361 tcatctgttt ttttgtgatg cgcctcgctc ccggctcgcc cacggatctg cagacgcaga
     9421 tgaacccgaa agcatcggcg gaaatgcgcc agcgcctgat gagcctctac gaactcgata
     9481 agcccatcca cgttcaatac gtatcctggc tcacaaagct gattcgcggc gacctgggaa
     9541 catccttttc atccgaccac cggccggtgg ccgacaagat tctggagcgt ctgccgatca
     9601 cgatcacgat caatcttctt tcgctggttc tgatcatcgc ggtggccgtg ccgatcggcg
     9661 ttctgtctgc ggtccatcag gactcgcttt tcgacaaggt gatgtcggtg attgttttta
     9721 tcggttttgc cgtgccgacc ttctggctgg cgctcctcat gatgattttt ttcggcatac
     9781 acctgggttg gctgccgatt tcaggattgc ggtccctcaa ttatgaatat ctgagcactt
     9841 gggggcagtg gacggatctg gccaaacatc tgatcctgcc ggtttttatc tctgctttcg
     9901 gagggctggc cggattgtcc cgctacatga gggccaatat gctggaggtg attcggcagg
     9961 actacattct gacggcgcgc gccaagggat tgagcgagcg ccaggtgatt tacaagcatg
    10021 ccctgcgcaa cgcgctcctg ccggcgatca ccattctggg gctgtcgatt ccggggctca
    10081 tcggcggcag cgtcatcttt gaaacgattt tcgcgattcc gggcatggga cagttgtttt
    10141 acatgtcggt tatggcgcgc gattatccga cggtgatggg gattttgctg atcggcgcgg
    10201 ttttgacgct ggcggggaat ctgatcgcgg atgtgtcgta cgcggcggcg gatccccgca
    10261 ttaggatttc ttaagggttt aagatgggct tccgggaaat gttttataaa aacaagctgg
    10321 cgctggcggg cctggcgatt gtggttctgc tttttgtgat ttccctggcg gctcccctga
    10381 tttcgccctg cgatccgggc gcaatcgatc tgcaaaacgt tctggagccg ccgtcggtag
    10441 agcattggtt cggcacggac cagctgggcc gcgatgttct ttcccgaatg atctggggcg
    10501 cgcgcatttc cctgaaagtc ggttttgtcg ccacggggct ggccattctc atcggaatgg
    10561 tcctgggggc cgtcgcgggc tattacggcg gctgggtcga tgcggtgatc atgcgctttg
    10621 tggacgtcat gctgtgcttt cccgctttct ttctgattct ggcggtgatt gccttcctgg
    10681 aaccctccat ctggaacatc atgatcgtga tcggactgac gggctggatg ggggtcaccc
    10741 gtctggtgcg ggcggatttt atttcgcttc gcgagcggga tttcgtatgg gcggccaggg
    10801 ccatcggcgc caatgatgcg cggattattt tcctgcacat tctgcccaac gcgctggcgt
    10861 ccattctggt cgcggccacg ctgggcattg ccggggcgat cctgacggag tcggcgctca
    10921 gtttcctggg catcggcgtg cagccgccaa cgccgagctg gggaaatatt ctgacggccg
    10981 gcaaggacaa catcgacatt gcctggtggc tgtcgctgta tcccggcctg gccattctca
    11041 tcacggtggt cgggtacaat ctgctgggcg aggggattcg tgacgcgtcc gatccgcgcc
    11101 tgaaaagatg aaaagccgat gcgcccgtcc aaacgtccgg ggcttgatta tgtcggcaaa
    11161 atgtaatact agagcgccac ggttgcagag ggtgcagccg gggaactttg tcgaggaatt
    11221 caggaggatc ccaatgtcca aaagagtaaa cccggaattg ttgaagatag accgcagaat
    11281 cattgcgcgc aagctgctga tcggagaatt gtcggaaaaa gatctgtcca gccttttaaa
    11341 aaagcttccg gacgtttcgg aaaacgcaga agaaattcag attcaacccg gcgaaaagcc
    11401 ctcatgctga tcgattccca tgcccacctg gagatgaagg agttcgatcc cgatcgatcc
    11461 gacgtgattg aacgcgcccg ggcggcgggg gttgactgca tcgtcacggt ggggaccaat
    11521 ccggctttga gccgcaaagc gctgtcgatt gcccgccgtc atgaaaacat ttattcgacc
    11581 gtcggcatcc atccccatga tgcggccgcg gcggacgact cttcgctcga cgagctgaaa
    11641 aagcttgcgc aggacccgaa ggttgtcgct tacggagaaa tcggacttga ttatttccgt
    11701 gatctttccc cgcgtgaaaa gcaggcggag atgtttgccc ggcaattgga tctggccggg
    11761 gagctgcgcc tgccggtcat cattcacgat cgcgaagcgc acgaacagac gctgcgaatg
    11821 gtaaaagcct ccggcatccg ctcgggtgtc tttcactgtt tttccggcga ctggtccatg
    11881 gccaggcagt gcctcgattt gggattttat ctttccgtgc ccggcgtcgt tacttttgac
    11941 cggtccaaag tgctgcagga agttgtccgg caggcgccga tggactggct tcttctggaa
    12001 acagactgcc cctatctcgc gcccgtgccc catcgcggca aaagaaacga gccttccttc
    12061 cttgtccata ccgcgaggaa agtcgcccaa atcaaaggtc ttgcctggga ggacgtggcg
    12121 cagaccgcgg cacggaatgc ctgcaggctt ttttgcattc cccctccggc cttgaaatag
    12181 cgcgggcgaa attcttcaaa atctgctaca ggtttttcca tgaacgatcc gtccatcaag
    12241 acccgcccca gagcggtggt gctcctatcc ggcggtgtgg attccgccac gacgctggcg
    12301 attgcgcgga atatgaaatt tgacgtgcat gccctgacct tccgttatgg ccagcggcac
    12361 atggttgaac tggaagcggc cgcgcgcgtc gcccgggaaa acggcgtggt cgagcaccgg
    12421 acggtcgata ttgatttgcg gtgtattggc gggtcggctc tgacggcgga catcgccgtt
    12481 cccaaacacg gctcggtgga agaattgggg aaaaacattc ccatgaccta tgttccggcc
    12541 agaaacacga ttttcctgtc ttacgccctg gcctgggcgg aggtgatcgg gtcctccaat
    12601 attttcattg gcgtcaacgc tctggactac agcggatacc cggactgccg tcctgaatat
    12661 atcgaagctt atgaacgcct ggccaacctg gcgacccggg ccggcgtgga aggtacagag
    12721 aaattacgga ttcacgcgcc gctgattcat tggagcaaag cgcaaattgt caaaaggggg
    12781 atggagctcg gcgtcgatta ttcgctgaca cacagctgct acgacccgga tgcggacgga
    12841 gcgtcctgcg gccgttgcga ttcctgcctg ctcaggctga aaggatttaa ggaagcaggc
    12901 attaaagatc cgggaagata cagattgaag accgaaggct gaaggtttga aggcttttgc
    12961 cttcggtctt cagtcttcag cctaaaacgc ttatgtattc tattaaggaa atcttttact
    13021 cgatacaggg agaaggctac cacaccggcc gtccggcggt tttttgccgt tttgccggct
    13081 gcaatctttg gtctggcagg gaagaagacc gttccaggtc gatttgcaaa ttctgcgaca
    13141 ccgattttgt cgggacggac ggcatcaacg gaggcgtcta tcgatcctct gccgaactgg
    13201 cgcacaggat ctccctggcc tggccggtca aaaaaaataa gctcaccagg ccttttgtgg
    13261 tctgcacggg cggggagccg cttttgcagc tggatgccgc gttgatttcc gccctgcata
    13321 aagaaaactt tgaaatagcc gtggaaacca acggcactct tcccgcgccc gccggtttgg
    13381 actggatctg cgtcagcccg aaagccgacg ccccgctgaa acaggccgcg ggagacgaat
    13441 tgaagctggt tttcccgcag gagggcgccg agccgaaaaa gtacgaacgc ctggcgtttc
    13501 aacatttttt cctgcagccg atggattctc cgcacggtgg agaagccgtg aggctttcgc
    13561 ttgcgtacgt tctggcgcat ccccggtggc gaatcagtct gcagatgcac aaagttttag
    13621 gggttcgata acgatgatgg aaatcttcaa ggtttttcat ttcgacgcgg cgcactcgct
    13681 tccccgcgta cccgagggac acaaatgcgc gaagatgcat ggtcattctt tccgcgtcga
    13741 aattcacctt cgcgggaaag ccggttcaga cagcggctgg gtgacggatt ttgccgatat
    13801 tgccgccgct tttgagccta tcctggagca actggatcac aaaaatttga acgccgtcga
    13861 gggtctggaa aatcccactt cggaaaatct ctgccgctgg atctggctgc gcctgaaacc
    13921 cgtcctgccg cagttgtcca ggatcgttct tcaggaaagc cctgaatccg gctgcatcta
    13981 cgaaggagaa gatgagccgg aagcggccat ttaatccgtt gctttttggc ggttaatgta
    14041 ctacttttcg tccacggggt gccgctcctt tttcaggccg gaaaagaaac ggctgagaat
    14101 ataccctttg aacctgaccc gggtaatgcc ggcgtaggga gtggcaatca ttctaaagac
    14161 aattccagcc atatcctttt caggtgacag ggtatggctt attttttttg gtaatccatg
    14221 aatcggtgca ttgtcattat cagcggcggg cgtcttggcg acatggactt tttccggaag
    14281 aaaatgtcgg aggcaggaag cgatttgttg attggctgtg acggcggcgc ccgtcatctg
    14341 gcggcagccg gggttacacc ggatgtgctg gtcggcgata tggattctat tgaatcttcc
    14401 cgtctggcgg attatgtgcg caggggtgtg aaaatcattc agcatccggc cgacaaagat
    14461 tttaccgata cggctctggc cctggattac gctctgggtc tgaagcccga aaggattcag
    14521 atctgggggg cgctgggcgg aagaatcgac catgctctgg ccaatttgtt tttgctggtc
    14581 cgggcgaagg aagcgggtgt tgccgcctgt ctgctggatg aatactgtga agtttttgtg
    14641 cccgttgatg atgcggtctt tgaaaacagg aaaggctgcc tcgtttccct cctggcgctt
    14701 tcgccccgcg tggaaggcgt tacgctggag ggatttgctt atccgctcga ggaaggaacc
    14761 tttgaacttt ccgaatcgcg cggcgtgagc aacatcattg ccgcagagcg cgcccgcatt
    14821 cgttttcgct cgggtcatct gctggtcatt cgctaccggc aaaaagattt ttttccggag
    14881 gcggactgaa tgggaaagcc catccgaatt ttatgcatcg gcgggtcgga ctccggcggc
    14941 ggggccggga ttcaggcgga cctgaaagcg gttcatgcct gcggcggtta cgccgtgtgc
    15001 gccgttaccg cggtgaccgt gcaaaataca cggggggtgc agtccgtttt gcccgttccg
    15061 cccaaatgga tagcggccca actggattcc gtgctggagg atatcggcgc ggacgctgtc
    15121 aaaaccggaa tgctcatgac gccggaagcg gtggagacgg tggccggaaa gatcaaacaa
    15181 taccggctga aaaatatcgt ggtcgatccg gtgatgatcg ccaagggcgg gcgctctttg
    15241 atgaccgaca gggccaggcg ggcgctcgtg aaaacgctgc tgccgctggc ggacgttctg
    15301 acgccgaata ttccggaggc cgaatttctg tcgcgcatga aaatccgttc cgtggaggat
    15361 atgaaaaaag ccgccgccgt cctttcggat ttaggcgtaa agcgggtggt ggtcaagggg
    15421 gggcaccttc ccggaagaaa gacccggggc tgcgtggata ttctttatga cggccttcgg
    15481 tatgatatgt ttgcggcaaa atggacgcaa acgaaaaaca cccacggaac cggctgcacc
    15541 tttgcttcgg tgctggcgtc ctgtctcgcg caggggcgcg ggacgcagga ggccgtccgg
    15601 caggcgaaga ggatgaccga aagggccatt cagcattcgc tgtcactggg caggggccac
    15661 ggtccggtga atctgtgggg ggatttcggg gtaaaaaagg agaagaagtc atgacgcagt
    15721 tggaaaaagc ccgaaagggc gttctcaccg aggaaatgaa acagagtgcg gcccgggaag
    15781 gcgtttcgcc ggaagccatc cgacagggca tcgtcgatgg caccatcgtg atctgccgca
    15841 atataaacca cacggccata gagccgctgg cgatcggtct ggggctgcgc accaaggtca
    15901 acgccaatat cggaacgtcc agggacaatc aggatttgaa gctggagctg gagaagttgt
    15961 ccgtggcgat cgcggcgggg gcggatgccg tgatggatct ttcgaccggc gggaatcttg
    16021 cggacattcg caaggccgta atggccaagt ccagcgtcgc catcggcacg gtgccgattt
    16081 atcaggcggc ggtaaaaaca ctggaagaga aaaaagcgat ttcagaaatg acgccggatg
    16141 atctgtttgg ggtgatcgaa gagaacggac gcgatggcgt tgatttcatc accgtgcatt
    16201 gcggcgtcaa tcgtgaaagc gtggctgccg tggaagcaca gggaagagta ttgggcatcg
    16261 tcagccgggg cggttcgatg acggccaact ggatgcgatg caacgaccgg gaaaatccgc
    16321 tgtacgagga gtatgaccgg cttctggaca ttgcccgccg ctacgatatg gttttgagcc
    16381 tgggggacgg cctgcggcca ggcgccatcc atgacgcgac cgaccagggg cagctgatgg
    16441 agttaatcat tttagggcaa ttggccgccc gcgcccgcgc cgccggtgtg caggtgatga
    16501 ttgaagggcc cggtcatgtg ccgattaccg aagtggaagc caacatcaaa cttcagaaga
    16561 aattatgtca gggcgctccg ttttatgttc tggggccgct tccgacggac attgcgcccg
    16621 gctacgacca tatcacttcc gccatcggcg gcgcgattgc cggcgccgca ggcgcggatt
    16681 ttctgtgcta cgtgacgccg gccgagcatt tgcgtctgcc cacgctggcc gatgtccgcg
    16741 acggcgtgat ggctgcgcgg attgccgcgc atattgccga cattgccaaa ggcatcccgg
    16801 gcgcttttga gcgggaccgg aaaatgtccg agtgccgcag gaaatttgac tggcaggggc
    16861 aggtgaaagc ctcccttgac ccggacaaaa cgtccgcgct ccttgaaaag agcaaaagcg
    16921 cgaatgagga aggctgcacc atgtgcggcg aattctgcgc gatccggctg ggaaagaaat
    16981 aaatccaatc gccacgagga aagaagattg atccgaaagg cggatgatca ttccatcgaa
    17041 aaacggctgg gcgcaaagct ggtcgtcgaa ggcctgcggc gggcggggtt tgaagcctat
    17101 tttgtgggcg gctgcgttcg ggattttgtt cgcggggttt ctccgggtga ctatgacatc
    17161 gccacgtccg cgccgccgga tcaggtgatc cgtctttttg aacgcaccgt cgccgtgggc
    17221 gccaaattcg gcgtggtggt ggtttgcgag ggcggatttt cctatgaggt ggccacgttt
    17281 cgcagcgacg aagcatatga ggacggacgc cgccccacgc aggttcgctt ttcatcggcc
    17341 cgggaagacg tcctcaggcg ggattttacg atcaacggcc tgctgatgga tccggaaacg
    17401 aacgacattg tcgattacgt cggcggccgg gaggacatcg gaaaaaaaat catccgcacg
    17461 atcggcgacc cggccgttcg tttcagtgaa gattatttaa gaatgctgcg ggcgatccgt
    17521 tttgccgccc atctggattt tcatctggag gacggcgtcc acgaagcgat ggtgcggcac
    17581 accgcgaaaa tccgggagat cagcgcggag cgcgtgcagg aggaattgaa taaaattctc
    17641 acgcggagcg gagcgcggcg gggtctggaa ttaatggccg atacgggtct gctgaagcac
    17701 atcctgccgg aagtggaccg gacgcggggg gtggcgcaac cgccgcgttt tcatccggag
    17761 ggcgatgtct ggcggcacac gctgatcatg atggagattc tgtcgaacga aaaccggccg
    17821 gaggcggagg cggcgctggc ctggggagcg cttttgcacg acgtcggcaa gcccgtctca
    17881 cggacggaag atgaaaaagg cgttcatttt tacgggcact cggtgacggg tgtatccatc
    17941 gcggaagacg tgatgcgccg cctgcggttt tcacgcgctc tgcgggaaac cgttctggac
    18001 ctgattcgtc agcacatggc tttcatgaac gtgcagaaaa tgcgtcctgc ccgcttgaag
    18061 cggtttctgc ggatgccgaa ttttcacctg catctgcagt tgcaccggct ggattgcctg
    18121 gccagccaca aaattctgga ccattatgcg ttctgtgtgg agcagctgca aaagacggag
    18181 ccggaagatc tgcatccgcc ccgtctgctg accggcgatg atctgctggc gatgggtttt
    18241 gccccgggaa aacaaatggg ggaaatcctg cgggcgctgg aagaagaaca gctggaaaac
    18301 aggattcatg aaaaagaaga ggcggtggcg tttgttcaga cacgatggaa attctagcgc
    18361 ggcgatgcgt gcaaaaggga agatgaagcc catcctcttg cggctggcgg tgcttttcat
    18421 gatgggcttg ctccttcagg cctgcgcggg tcgcgaagca aaagtatatc ccggttcgca
    18481 ccggccgcag aagcgtcagc tcgccgagat ggggtacacg attcaggcgg gcgcgtttgc
    18541 ccgggtggaa aatgccgtgc gcctgacgca aaggctcaaa ggccagggac tccctgccac
    18601 ctatttcagg gcggacgacg ggctgtttaa ggtgcgattc ggaaacttct cgacgaaaga
    18661 gccggcgcgg ctccgcgcgg agtctctcgc gcaggcgggc gtgatcgaag aatactacat
    18721 cgttttgccg gaagattccc cggcggccaa acgaaagcaa tacgggacgg attatttgcg
    18781 cgagtcgctg gtcaaaacag ctcacgattt tatcggcgtg ccgtatctgt ggggtggtgt
    18841 gtccgcggac accggctttg actgcagcgg cctcgccatg acggtctatc agctcaacgg
    18901 cctggatctg ccgcgccatt cgcgaacgca gttcgacgcg ggagattcga tcgcccggca
    18961 ggaactgcag aaaggcgatc tggttttttt tgacacgcgc ggaggtggaa aagtgtctca
    19021 cgtggggatt tacatcggcg ccggccggtt tattcatgcg ccgtccagcg gaaaaaaaat
    19081 ccgcatcgac tcgctcgcct ccgattacta tgcccggcgc ttcatcggag caaaaacgta
    19141 tctctgatcg gaaaactctg aaagtcttgc tgccaaagga ttacattgat tcattccgtt
    19201 gtgcttcgac cggatttctt ttgacggact tttcaattgc aaaaaaattt cttttttgaa
    19261 aaaaagaaaa aaattaaaaa tattttatag tttttgttga ccgaaagaaa aaaataaagt
    19321 aatattgcac cacatccgta agattacggg tttttgaaaa gatgaagtaa actcaattaa
    19381 aggaggctaa acccaatggc agcaacagca aagaaagtcg tcaagaaggc cgcagcgaag
    19441 aaaccggcag cgaaaaagaa agtcgtgaag aaggtcgcag caaagaaacc ggcggcaaaa
    19501 aagaaagttg cagcgaagaa gcccgtagcg aaaaagaagg tcgtgaaaaa agcagccaag
    19561 aagtaatatt gatcagtcat ctacatttaa agagcaggcg gacgcaagtt ccctgctctt
    19621 taagtatccg ctccatctct ttcttttccg acgatccgcc gcttccagag gtgattcatg
    19681 atgaaaatat gatgctccgg ccctgccggt cgcctggcgt caaggcgccg gtgccggagg
    19741 tcaagggggc ggtgaccggc ttcggcgatg tgcaccgccg cgcgcatctg tgccgggccg
    19801 ctggggaagg tccggcgtat gcgcaccgga aggccaaaga gcgtcaggaa aagcgcggcg
    19861 gccggaagcg gaacaggcgg taaacggtgg atagtggatg gggaagaaac ggaggatgag
    19921 ctgtctattg acagaatccg caaagcgaca tatatccata acgcacaaat cttgagagaa
    19981 aaggcaaatt cgatgctgga actacagaaa gtgtatttca ccgtacagga cactgaagga
    20041 agcgacggcg gtccgcgcaa catcattgac gggattgatt acgtttttga agacgggaag
    20101 ttttacgtca tcaccggccc gaacggcagc ggcaagacca cgctggccaa gttaatcatg
    20161 ggcgtcaacc cgttatccgg cggcaagatt cttttcaacg gacagaatat ctccaccctg
    20221 acgattaccc aaagggccaa agaaggcatc gcctacagct ttcagcagcc cgcccgcttt
    20281 aaaggcatta cgtttcgcga cctgctgacg attgccaccg gcatcgaaga ggaacccagg
    20341 cttctggaga ttctgggccg ggtgggcatt tgccccctgt cttttctgga caaggaagtg
    20401 gatgccaggc tgtccggggg ggaaattaaa aaaattgagc tggcgacgac cattgccgcc
    20461 aatcccaaac tggcgattta c
//
LOCUS       DAOSIM010000013        19672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_3017_length_19672_cov_11.086048, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000013 DAOSIM010000000
VERSION     DAOSIM010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 19672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19672
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3017_length_19672_cov_11.086048"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PLR47_01315"
     CDS             complement(<1..468)
                     /locus_tag="PLR47_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HOG11602.1"
                     /translation="MADKSKVGMVFPPYTFEVEKGKIAEFAMAVSQKESASQVKQIYA
                     DSGAAKAAGYKDIVPPSTFQTCFLLWGGGGLMPMLQAVGINLVRLLHGEEEYEYLLPI
                     HPGDVMTGVIKVVEMFDKEKKGKPGKFMEFTILEAEIRNQRGELAMRARTTLVE"
     gene            complement(514..1695)
                     /locus_tag="PLR47_01320"
     CDS             complement(514..1695)
                     /locus_tag="PLR47_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOG11603.1"
                     /translation="MAGPLEGLKILDFTTLLPGPYATMTLADLGAEVLSVVSGSRPDL
                     AAITPPFITGTDLSVASAYLGRGKKSITLNLKDARGVEVVHRLLAKYDILIEQFRPGV
                     MAKFGLGFSQIKEKYPSLIYCSLTGYGQTGPLKDRAGHDINYLSVAGLMSYAGRRDGG
                     PVPMPMQIADVASGSNSAIIAVLAAVIHRLKTGKGQHLDISMTDGVFAFNALAAAACL
                     AAGEIPEREGFILNGGSLYDFYETADHQYIGFGGMEPQFFAAFCRAIGRPDLIDGTFL
                     PPNLSEVKKEVRAVISGKTRKEWESIFDKVEACVEPVLSLSEAINSSLAGQRNWIVDV
                     TLPDGTTVRQPACPLKFSATPPVYGKTGVKAGSHNREVLLEVGYTEAEINEFGKTGLF
                     S"
     gene            complement(1706..2848)
                     /locus_tag="PLR47_01325"
     CDS             complement(1706..2848)
                     /locus_tag="PLR47_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG11604.1"
                     /translation="MDILQYTDEHRMFRDTFRKFVTNELIPHIEEWEEAGIVPKSVWK
                     KMGEHGFLGMSVPVEYGGAGADFLYSVIVAEEMAYANFSGLAASLHTDIVVPYITSFG
                     SEEQKEKYLPGCVTGDIITAVAMTEPNTGSDLAAMKTTAVEAGDDIILNGQKTFISNG
                     INCDLCVVAAKDPTCDDPHKAVDLYLVEAGTPGFDKGKQIKKAGWHSQDTSELYFTDC
                     RIPKSNRLGEKGTGFMKLMTKLQQERLVVAIGVIAQAEYLLDITTKYCRERTVFGKPV
                     SKFQVNQFTLVECATEIAIGRTFVDKLIVDHMEEKQIIIETSMAKYWITDMACNVADR
                     CLQLHGGYGYCDEYGISRAWRDLRVTRIFAGTNEIMKVIIARFMGL"
     gene            complement(2990..4204)
                     /locus_tag="PLR47_01330"
     CDS             complement(2990..4204)
                     /locus_tag="PLR47_01330"
                     /inference="COORDINATES: protein motif:HMM:NF004810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HOG11605.1"
                     /translation="MSSGIRDKVAIIGMGCSRFGERWDIGAEGLMVEAFQEAIADAGI
                     EKKDIQAAWYGVCMDEVNVGKGAPSMGLALRLPNIPISRMENYCATGTEAFRGAVYAV
                     ASGAYDICLALGVEKLKDTGYGGLPAWGSTAGTLVWQFFPNATAPGLFAQLASAYAAK
                     FKIKNDDIKQAIAHVSAKSHANGALNPKAHLQKAVTIDQVLAAPIIAWPLGLFDCCGV
                     SDGSACAIVTTPEIAQKLGKKKKDLVTVKAVQLAVSNGYEGGFNEWDGANFVTTDKCA
                     AATYKEAGITNPREEISMFECHDCFSITELVTYEDLHLSERGQAWKDALNGMYDRDGK
                     IPAQIDGGLKCFGHPIGASGLRMIYEMYLQLQGRADKRQLENPRYGLTHNLGGFPHQN
                     VCAISIVGKYGK"
     gene            complement(4220..6940)
                     /locus_tag="PLR47_01335"
     CDS             complement(4220..6940)
                     /locus_tag="PLR47_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG11606.1"
                     /translation="MIGITSYGAYIPRLRLDRMAIYQSMGWFAPALISVAQGERSMCN
                     YDEDSLTMAVAAAMDCLIGKDKTKVDAGYLCSTSLPFLDRQNAGILATALNLRSDINT
                     ADFTSSQRAGTTGLLTALDVVKSGSRKSILVTASDKRQTRPAGFYEMWFGDGAASVLV
                     GDEDVVAEFLGSYSLTYDFVDHYRGSKSEFDYTWEERWLREEGYSKIIPEAVNGLFKK
                     LNISMNDVQKLVFPCLFKAEHRAIGKKLGAAPEKLADTMHEVTGETGVAHALLMLVGA
                     LETAKPGDGILLAGFGQGCDALYFKVTENIGKLAERAGLKGSLANKKTTDNYMKFLKF
                     RDLIDPEMGIRAEAPVQTAMTALWRNRKMVLGFVGGKCRDCGTAQFPLADLCVNPACG
                     HLGTLEDYEFANVKARVRSFTGDLLAVSVDPPAIYGMVQFEGGGRLMMDFTDSELNDI
                     KVGMPVKMSFRRRVVDTVRGFSQYFWKAVPQTDWIPEIDFQGRVAIVTGAGGGLGRAY
                     ALELGRRGAKVVVNDFGGARDGSGSGTAGPADVVVADIKAMGGEAVANYDNVATPEGG
                     QNIVKTALDAFGKVDILINNAGILRDKGILKMEPENWSAVLSVHLNGAYNVTRPAFEA
                     MRNNGYGRIIMTTSAAGLYGNFGQTNYSSAKLGLVGFMNTLKLEGAKYNIKVNTIAPI
                     AASRLTEDVMPPEMLEKMNPDYVSGIVLYLSSEDCKDSGNIFNAAAGFYSRAAILTGP
                     GVMLGDDKHVPTPEMIRDNWEKINSLEGAKPYTELNMAVMDFLTPPKANGGEATGGGG
                     GGIDVKGVFEKMPSVFNAAAAAGKDVTFQFNISGPAGGDWFVAVKDGACSVTAGKGEN
                     PTTTIGMADTDFVDLITGKLDGMKAFSAGKLKVGGDMMKSQLIGKLFKFN"
     gene            complement(7012..8289)
                     /locus_tag="PLR47_01340"
     CDS             complement(7012..8289)
                     /locus_tag="PLR47_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG11607.1"
                     /translation="MMGASKEIRFDGRVAIVTGAGGGLGRVYALEFGRRGAKVVVNDF
                     GGARDGSGEGAAGPADKVVDEIKALGGEAVANYANVATPDGGESIVKSAVDAFGKVDI
                     LVNNAGILRDKGILKMEPENWQAVLDVHLNGAYHVTRPAFSVMRDNGYGRIIMTTSAA
                     GLYGNFGQSNYSSAKLGLVGFMNTLKLEGVKYNIKVNTVAPIAASRLTEDILPPDLLA
                     KMNPAYVAPIVLYLCSEECNETGRIYNCGAGFYNRAAMMTSPGIVVGDGKKVPSVEDV
                     SVNWAKIMDLKGAKEYGQLNDLVLQDLLSALEGKKEPVQSGGGFTAVKEVFEKMPGAF
                     QASAAGGAAVVFQYCILGDGGGDWSADIKNGACAVVPGVHASPTCTLKIQAGDFLDLM
                     NGKVNAMKAYTSGKLKIEGDIMKSQLLEKIFKF"
     gene            complement(8367..9350)
                     /locus_tag="PLR47_01345"
     CDS             complement(8367..9350)
                     /locus_tag="PLR47_01345"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HOG11608.1"
                     /translation="MKTAITEMFGIQYPIICGAMMWLCKPTLCAAISNAGGMGNLTAA
                     NYETEEDFRNAIRETRKLTDKPFMVGLTILPSVRITPGHHQMYLRVCAEEKVAGIEVS
                     GAPVDKAAGPEYIDMLKKAGVKLFHKVGAVRHALHAEKAGYDGIYAAGIEEGGHPLDD
                     DVTTMVLTPRIAESVKIPVVTVGGIANGKSLAAALSLGAQGVMMASRFIATKECVVHD
                     NIKEEIVKRQEYETTLICKSLKLQGRALKNQVVREICEIEEKGGGFEALYPLIAGDRM
                     KEAWEDGDVEVAPLMVGQSIGLIKDVPTCKELLERMVQEAQDTLAGVKNMF"
     gene            complement(9439..11307)
                     /locus_tag="PLR47_01350"
     CDS             complement(9439..11307)
                     /locus_tag="PLR47_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG11609.1"
                     /translation="MQKYLVDHRDLQFALFEFLEVDKMNRFRRFEDFNRDVYEETIRL
                     AEKIASECVFPANVNGHKEGCHYDPQTRSVRLPAGYQAAAKAIIDAGFLSIADDPEIG
                     GAGMPLAIQSCAWEIFCGAGGALMLFFMLGHGAAHLIHTFGTSRQKSIYVHKILSGEW
                     GGTMCLTEPEAGSDVGALKTKAVPQPDGTYKITGQKCFITNGDQDITRQIIYPVLARI
                     EGDPPGVKGISIFIVPKYLVNEDGSLGERNDVFCTGLEHKMGAHGAATCSMSFGENEN
                     CTGYLLGERGRGMNIMFQMLNETRLEVGIMGLGGSSAALQFAVDYARTRLQGVHFSKM
                     FETNAPKVPIIEHPDVTRMLMGMKALVEGQRMLSYYAAMQIDLAKLLEGEESREARSL
                     VDVLIPLVKAGNAENTWHITAEAIQVHGGYGYCSEYPVEQLAVDCKSLSIVEGTNGIQ
                     SLDLVMRKILLNADQKNYKVLKSRIFQTIEKANGIVEDVYRNMLLTGLERMNSTLETM
                     KKNIDEGRYHTLLLNVDPVRRAFFDLMLGWMHLWCLALVRPKLSSLTAGAKGEALTEI
                     LERDAEAAFYYGKACVSEFWLATEFPKYFGKLEGISLGEKVDFLPGNTMFLSHPCA"
     gene            complement(11337..12140)
                     /locus_tag="PLR47_01355"
     CDS             complement(11337..12140)
                     /locus_tag="PLR47_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOG11610.1"
                     /translation="MSTLMPEFKTLLFEEKEPHIGVVTMNRPGQLNAINLDMLNDFER
                     LFQALSGEDSIRVLILTGTGKGYSSGADLNDAVIHKESEAFADPEKFLKLVQERYAAL
                     ILGMRRIPQPIISAVNGPAAGGGFSMTLASDIRVACKEAYFVASFINIGLSGGELGCS
                     FLLPRLVGLARASDILFTGRKVGAEEAERIGLVNKLVPREELLEAALSYARSMTGKSV
                     GGLKLTKRVLNENLHAPSLETAVNLENRNQTIMVFSGEVFKLVQKFYKT"
     gene            complement(12137..13816)
                     /locus_tag="PLR47_01360"
     CDS             complement(12137..13816)
                     /locus_tag="PLR47_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOG11611.1"
                     /translation="MSFERIWHKSYAPGVPAEITVEKITMAEVLTRTAATYPDRVAMI
                     YMGKKITYRQLEKLVNRFTRALIDIGVREGDKVAMLLPNIPQIIIADHAAYRAGAVTA
                     MNNPLYTERELIHQLYDSDATVLVTLDLLLPRALKIKGETKIQKIITCHINDYLPFPK
                     KQLFPYVKKTMFYKVDPHPGVYEFMDLIAKYPDTPVENKAKWNSLAALIYTGGTTGVS
                     KGAMLTHANISSVVQQFSVWFPDLKGQYQNLTGIYPVFHSAGYSVSQNLPIWNGWGCT
                     LIPKPEPGVIVEMLEKFHPTFLTGVPTIYTSLLDNEKFRKMNLSYVRGYFGGAAPLPE
                     NTLKQLKDLHGAIIYDVYGATENTAFATTTPWGGKVKVGTVGLPLPNTDIKIVDVDTG
                     TRELPVGEHGEICIKGPQVMTGYYNKPEETANSLRDGWFYTGDIGYFDEEGYLTISDR
                     KKDVIVASGFNIFPKEIDEILFEHPKIHEACCIGIPDGYRGETVKAYIVAKPGETLTK
                     QEVVAFCKERMTAYKVPTEVEFIAELPKSAIGKIMRRELRDMDREKRGETS"
     gene            complement(13969..14775)
                     /locus_tag="PLR47_01365"
     CDS             complement(13969..14775)
                     /locus_tag="PLR47_01365"
                     /inference="COORDINATES: protein motif:HMM:NF004794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOG11612.1"
                     /translation="MENESSFKVEKTGHVAWLILNRPKQRNTMTLEFFQELPRLFKEF
                     DDDNDVRVVVIRAEGKSFTVGLDLVAAQSLLGDGSAVYREELRRKIYDLQESMSAIEK
                     CRKPVIVAIHGHCIGGGVDLTSACDIRLASKDAIFSIRETRIGIIADIGTLQRVPYII
                     GQGWFRELALTGRDWTAEESLKMGYITRLCETPQVLFEEARTLAEEIANLPPLAIQGI
                     KEVANYSRDHGIRQGLEYVAQRNAALVPNDDMVEAVMAFLEKRTPKFHGR"
     gene            15143..15487
                     /locus_tag="PLR47_01370"
     CDS             15143..15487
                     /locus_tag="PLR47_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11613.1"
                     /translation="MEEYVHQTIGEEVRFIAGHYQIVEEGRLTHDGREFLYWMGIATV
                     DNACCGHTGCRFLLIPGYIHSWKTKTNAQGKPVSEVKPIVDERERAAIRTFLEACYPY
                     AQINFSIKSQLV"
     gene            15670..16377
                     /locus_tag="PLR47_01375"
     CDS             15670..16377
                     /locus_tag="PLR47_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG11614.1"
                     /translation="MKSPLEKARKNPKSMKAKILHAARRIFGEYGFHGTTTRMIAKEV
                     GIDISTLHYHWGDKNDLYEAVVVDVNNDLGRELRLVENIVHGSSLEKRLNRSIDHMMD
                     YLFDHPEISNLILLRYFSKTRNEANVDLKVPEFSGDIARAMDLTKDAKNPPPRAMMEV
                     LGIMNGIHSFVSGENFFRPMLNLNRENYIRAVKETLKFLLVPAFVLREKQTKKAKNGS
                     LSKKTEEKKKNQIKKSK"
     gene            complement(16829..18748)
                     /locus_tag="PLR47_01380"
     CDS             complement(16829..18748)
                     /locus_tag="PLR47_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG11615.1"
                     /translation="MKLLVGGVLLVLIPLLVVGVFSAVRSSQALQQNAREESEQIASS
                     LAEMAQMVLLEELKIVKQLSAYPAVVAVADKQATGAVPVSELEAMNATLTEIMKKIGN
                     DYDQLYLVDKNGKIFCDDVGGKLKGLDVSARDYYLAGKGGEVFVGNVTKSKATGNTVS
                     SVSAPIYSQGGEFLGVAAAAMKVDFLVERITDTKVGKTGYPFMIDRAGMVIAHPKKEY
                     ILTLDLTKEAEMTEIVGKMTGKQKGSESYVFKGDKKICGFAPVEITGWSVGFTQNTDE
                     FLAAAHGIRNVIAIISVIFLGVAVFAIIVFARRISVPIARVANELNDAAAQLTSASSE
                     VSSASQSLAEGASEQAAAVEETSSSLEEISSMTRQNANSAQLANDMMLKEAAPNFQMI
                     GERLEIMEKAMQASVSASEETAKVIKTIDEIAFQTNLLALNAAVEAARAGEAGAGFAV
                     VADEVRNLAMRATEAAKNTQDLIGNSTGRIKEATSFYGQVSEAMAKNSDIAKKVTELS
                     GEIAAASREQAQGITQINSAVVEMDKVVQQSAANAEETASAAEEMNAQAEQIRHVAER
                     LLEVVEGGEEKGGGGMVAALNRRRGAVVPPDRTAATTTRKNIPVPRKRTEAERLIPFG
                     DKEKGTHEGGEFHDF"
     gene            complement(19232..>19672)
                     /locus_tag="PLR47_01385"
     CDS             complement(19232..>19672)
                     /locus_tag="PLR47_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11616.1"
                     /translation="QDLRDGTPPETAMQQLLNTYPLFGYCRQAVTAEDKKILMEIASS
                     RQYELPMRRFALKLLQQFVGDQDVKEYLRALWDKPCDYEVRLEVLWGLLNYVEASEEL
                     YAEIGMHFDAANWDKWLPLIVEKLEGDAEEKVHVRELMKRFFNL"
BASE COUNT         4351 a         5789 c         4776 g         4756 t
ORIGIN      
        1 ctccactaaa gttgtgcggg cgcgcatcgc cagttccccg cgctgattcc tgatttccgc
       61 ttccagaatg gtgaactcca tgaatttacc cggcttgcct tttttctcct tgtcaaacat
      121 ttcgacaacc ttgatgactc cggtcatcac atcgcccgga tgaatgggaa gcagatactc
      181 atactcttct tccccgtgaa gcagccggac caggttgatc cctacagcct gcagcatggg
      241 catcaacccc ccgccgcccc agagcaaaaa gcacgtttga aacgtcgaag gcggaacaat
      301 atccttatac cccgccgcct tggctgctcc ggaatcggca taaatttgtt tcacctggct
      361 tgcgctctcc ttctgcgaaa cggccatagc gaactcggca atctttccct tttccacctc
      421 gaaagtataa gggggaaaca ccatccctac ttttgatttg tcggccatgc ttacctccta
      481 ttatgatggt taatcatgat cggaaccttc tcttcatgag aacagtcccg tctttccgaa
      541 ctcatttatt tccgcctcgg tgtaacccac ttcgaggagg acttcccgat tgtgcgatcc
      601 cgccttgacg cccgttttcc cgtataccgg gggagttgcc gaaaacttca aaggacaggc
      661 cggctgacgc acggtggtgc catccggcaa agtaacatca acaatccagt tccgctggcc
      721 ggccagagag ctgttgatcg cctccgacag agaaagcacc ggctcaacac aggcttccac
      781 cttgtcgaat atactttccc attccttcct ggttttcccg gaaatcacgg cccgcacctc
      841 tttcttaact tcggacaggt taggcggcag aaaggtgccg tctatcagat cggggcgtcc
      901 gatcgcccgg cagaaggcgg caaagaactg cggttccata cctccgaaac caatatattg
      961 atgatccgcc gtctcataaa aatcataaag cgaaccgccg ttaagaatga acccttctct
     1021 ttccggtatt tcgcctgccg ccagacacgc ggccgccgcc aaggcgttaa aagcgaaaac
     1081 cccatccgtc atggagatgt cgagatgctg ccctttgccg gtcttgagcc tgtgaatgac
     1141 ggcggctaaa acggcgatga tcgcgctatt ggaccctgac gccacatcgg caatctgcat
     1201 gggcatgggg acaggtccgc catccctgcg tccggcgtag ctcatcagtc ccgccacaga
     1261 caaataattg atatcgtgcc ccgctctgtc tttaagcggc cccgtctggc cataacccgt
     1321 cagagaacaa tagatcagag acggatattt ctccttgatc tgggaaaatc ccagaccaaa
     1381 tttcgccatt acccccggac gaaattgttc aatgagaata tcgtatttgg ccaaaagacg
     1441 atggacgacc tcgacgcccc tggcgtcttt caggttgagt gtaatggatt ttttcccgcg
     1501 gcccagataa gccgaggcca cggacaggtc tgttcccgtg ataaacggcg gcgtgatggc
     1561 cgccagatcg ggccggcttc cggaaacgac ggacaggacc tccgctccca gatcggcaag
     1621 cgtcatcgtt gcatacggcc cgggtaaaag agtcgtaaaa tcgagaattt ttaaaccttc
     1681 cagtggtccg gccatgttcc ctcccttata atcccatgaa ccgggcaata ataactttca
     1741 tgatttcatt ggttccggcg aagatccggg tgacccgaag atcgcgccaa gccctggaaa
     1801 ttccgtattc atcgcagtac ccgtagcccc catgaagctg gagacaacgg tcagcaacat
     1861 tgcaggccat atccgtgatc cagtatttgg ccatcgatgt ctctataatg atttgcttct
     1921 cctccatgtg atccacgatc agcttgtcca caaaggtccg gcctatcgca atttccgtgg
     1981 cacattccac caacgtgaac tggttgacct gaaacttgga aaccggcttt ccaaaaaccg
     2041 tccgctcccg gcaatacttg gtggttatat ccagcaggta ttcggcctgc gcgatgacac
     2101 ctatggcgac taccagacgc tcctgttgaa gcttcgtcat gagtttcatg aatccggttc
     2161 ccttttcgcc caggcgattg cttttgggaa tcctgcagtc cgtgaaatac agctcggagg
     2221 tgtcctggct gtgccaaccg gcttttttga tctgcttgcc cttatcaaag ccgggagttc
     2281 ctgcctccac caaatacaga tccaccgctt tgtggggatc gtcgcacgtc gggtctttgg
     2341 cggccacgac acagagatca cagttgatcc cgttgctgat aaaggttttc tggccgttta
     2401 ggatgatatc gtccccggct tccacagccg ttgttttcat ggcggccaga tcgctcccgg
     2461 tattgggttc cgtcatggcc accgccgtga taatgtcgcc cgtgacgcaa ccgggaagat
     2521 atttttcctt ctgttcttct gaaccaaagg aggtaatgta aggaacaacg atatcagtgt
     2581 gcagcgacgc ggccagtccg gaaaagttgg cataggccat ctcctcagcg acaatgaccg
     2641 aatacaggaa atccgccccc gcgccgccgt attccaccgg aacggacatg cccaaaaacc
     2701 cgtgctcccc cattttcttc cagacgctct tgggaacgat accggcctct tcccattctt
     2761 cgatatgggg aattaattca tttgtaacaa acttccgaaa agtatcccgg aacattcggt
     2821 gttcatccgt atattgaaga atatccattt attcgtctcc tcatcaaaat gcattgtccg
     2881 gacgcttccg acccctctgc aaaaaccgat cggatcggag gaggccgcga aggtggagaa
     2941 agctgctccg ccacctccgc ggctcgagcg tcatccgcat ccaacagaat tattttccat
     3001 actttcctac aatggaaatc gcgcacacgt tctggtgagg gaaaccgccc aggttgtgtg
     3061 tcaaaccgta tcgcggattt tccagctgac gcttgtccgc ccgtccctga agttgcaggt
     3121 acatttcata aatcatccgc agccccgagg cgccgatggg atgaccgaaa cacttgagac
     3181 cgccgtcaat ctgggccgga atcttaccgt cccggtcgta catgccgttt aaggcgtcct
     3241 tccaggcctg accgcgctcc gagagatgga gatcctcata cgttacgagt tccgtgatgg
     3301 aaaaacagtc atgacattca aacatgctga tctcctcgcg cgggttcgtg atcccggctt
     3361 ccttgtaagt agctgccgcg catttgtccg tcgtaacgaa gttcgctccg tcccactcat
     3421 tgaagccgcc ttcataaccg ttgctgaccg ccagctggac cgccttcacg gtaaccaggt
     3481 cctttttctt tttgcccagt ttctgggcaa tctccggcgt cgtgactatg gcgcaggccg
     3541 agccgtcact gaccccgcaa cagtcgaaca atcccagagg ccaggcgatg atcggagccg
     3601 ccagaacctg atcgatggtc accgctttct gcaggtgcgc tttgggattc aaggcgccgt
     3661 tggcgtggct tttcgcagaa acatgagcga tggcctgttt gatgtcgtca tttttgattt
     3721 tgaacttggc agcataggcg gaagccagct gggcaaacag ccccggcgcg gttgcattgg
     3781 gaaaaaactg ccacacgagg gttcccgccg tcgaacccca ggcggggagt ccgccatacc
     3841 ccgtatcctt caatttctcg acgccgaggg ccagacaaat atcgtatgcg cccgacgcga
     3901 cggcgtaaac tgcgccgcgg aacgcctctg tgcccgtggc gcaatagttt tccattctgg
     3961 aaatgggaat attgggcaga cgaagagcca gacccatcga cggagcaccc ttgcccacat
     4021 tgacctcatc catacaaacc ccgtaccagg ctgcctgaat atccttcttt tcaatgcctg
     4081 catcggcaat cgcttcctga aaggcctcga ccatcagccc ctcggcgcct atatcccatc
     4141 gctctccgaa ccggctgcat cccatgccga taatggcgac tttatctctg attcctgaag
     4201 acataacaca ccctcctcat tagttgaatt tgaacagctt tccgatcaac tgggatttca
     4261 tcatgtcgcc gccgaccttt aatttcccag cgctgaaagc cttcataccg tcgagcttcc
     4321 cggtaattag gtcaacgaaa tccgtatcgg ccatgccgat ggttgtcgtc ggattttctc
     4381 ccttcccggc cgtgacgctg cacgctccat cctttacggc gacaaaccaa tccccgcctg
     4441 cggggccgga aatgttgaac tggaaggtca catccttacc ggcggcggcc gccgcattga
     4501 atacggaggg cattttttca aagacaccct tgacatcgat gccgccccct ccacctccgg
     4561 ttgcttcacc accgtttgcc ttcggaggcg tcagaaaatc cataacggcc atattgagct
     4621 ccgtataggg cttcgcccct tccaggctgt tgatcttctc ccagttgtcg cgaatcattt
     4681 ccggcgtcgg cacatgcttg tcgtccccca acatgactcc ggggccggtc agaatagccg
     4741 cccgactgta gaatccggcc gccgcgttga agatgttgcc ggaatctttg caatcttcag
     4801 agctcagata caggacgatg cccgagacgt aatccgggtt cattttctcg agcatttccg
     4861 gcggcatcac gtcttccgtc aatcgggacg cggcgatggg ggcgatcgta ttgactttga
     4921 tattgtattt cgcgccttcc agcttcaacg tgttcatgaa accgacaaga cccagtttgg
     4981 ccgaggaata atttgtctgg ccgaagttgc cgtatagacc ggctgcggaa gtcgtcatga
     5041 taatgcgacc gtacccgttg ttgcgcatag cctcgaaagc cggccgcgtc acgttatagg
     5101 cgccgttgag atggacggag agaacggccg accagttttc cggctccatt ttcaagatgc
     5161 ctttatccct gaggatgccg gcattgttga tgagaatatc gactttgccg aatgcatcca
     5221 gcgccgtctt gacgatattc tgaccacctt cgggggtggc gacgttgtcg taattggcga
     5281 cggcttcacc gcccatggcc ttgatatcgg caaccaccac gtccgcggga ccggcggtcc
     5341 ctgaccccga tccgtcacgc gcgcctccga aatcgttcac gacaaccttt gctccgcggc
     5401 gacccagctc cagggcatac gcccggccca aaccaccacc tgcgcctgtt acaatggcca
     5461 ctcgcccctg aaaatcgatt tccggtatcc agtccgtctg gggaacggcc ttccagaagt
     5521 attgcgagaa tccccggacc gtatccacga cccgtctgcg gaaagacatc ttgacgggca
     5581 tgccgacttt gatgtcgttc aactcactgt cggtgaaatc catcatgagc cggccgccgc
     5641 cctcaaactg caccatgccg taaatcgccg gtggatccac cgatacagcc agcagatctc
     5701 ccgtaaacga gcggactctg gccttgacat tggcgaattc gtaatcctcc agggttccca
     5761 ggtggccgca ggccggattc acgcaaagat ccgccagggg aaactgagcc gtcccgcaat
     5821 cccggcattt tccaccgaca aacccgagga ccattttccg gttccgccac agagccgtca
     5881 tggccgtctg cacgggcgct tccgcccgga ttcccatctc cggatcgatc agatcccgga
     5941 atttcaggaa cttcatgtag ttgtccgtgg tcttcttatt ggccagcgat cctttgagtc
     6001 ccgcgcgctc ggccagtttt ccgatattct ccgtaacctt gaaataaaga gcgtcacagc
     6061 cctgtccgaa accggccaga agaatgccgt ctcccggctt tgccgtttcg agcgctccga
     6121 ccagcatcag gagagcatgc gcaacgcctg tctctccggt gacttcgtgc atcgtatcgg
     6181 ccagtttttc cggagcagcc cccagctttt tcccgatcgc ccggtgttcc gccttgaaca
     6241 aacaggggaa aaccagtttt tgcacgtcat tcatcgaaat attcaatttc ttgaacaggc
     6301 cgttcaccgc ttcgggaatg attttggaat agccttcttc cctgagccac ctctcttccc
     6361 aggtgtagtc gaactcggat ttggatcccc ggtaatgatc cacgaaatcg taggtgaggg
     6421 agtaggaacc caggaactcc gccacaacgt cttcatcccc gaccagaacg gatgcggcgc
     6481 cgtcgccgaa ccacatttcg taaaaaccgg ccggacgggt ctgccgtttg tcggacgcgg
     6541 tcaccaggat ggatttgcgg ctccccgatt tgaccacatc gagagcggtc agaagtccgg
     6601 tcgtgccggc ccgctgggaa gacgtgaaat ccgccgtatt gatgtcgctc cgcagattca
     6661 gagccgtagc cagaatgccg gcattctggc gatccagaaa cggcaggctc gtggagcaga
     6721 gatatcctgc atcgaccttc gttttatctt taccaatcaa acaatccatg gccgcggcga
     6781 cagccatcgt cagactgtcc tcgtcgtaat tgcacatgga acgttcgccc tgggccacac
     6841 tgattagcgc cggagcgaac cagcccatcg attgatagat tgccattctg tccaatcgca
     6901 ggcggggaat ataggctccg tatgaagtga ttccgatcat cgttctacct ccttcgttca
     6961 caagacattg cattcaaatt atttaaaccg atgcttcaga aaatttccgc tctagaactt
     7021 gaagatcttc tccaaaagct gggatttcat gatatctccc tcgattttca gctttcccga
     7081 ggtgtaggcc ttcatggcgt tcaccttgcc gttcatcagg tcgaggaaat cccccgcctg
     7141 gattttcagc gtgcaggtcg ggctggcatg gacgccgggc accacggcgc acgcgccgtt
     7201 tttgatgtcg gcagaccaat ctccgccacc gtcacccagg atgcagtact ggaaaacaac
     7261 cgccgctcct cccgccgcgc tggcctgaaa ggctccgggc attttttcaa agacctcctt
     7321 caccgcggtg aatccgccgc cgctttgcac cggttctttc ttcccttcca aagccgagag
     7381 gagatcctgc aggaccagat cgttcaattg gccgtattct ttggccccct tgagatccat
     7441 gattttcgcc cagttgacgg aaacatcttc gacggacgga acctttttgc cgtccccgac
     7501 gacaatgccg gggctggtca tcatggcggc gcgattgtag aatccggcgc cgcaattgta
     7561 aattctgccg gtctcattgc attcttccga gcacaggtac agcacgatgg gagcgacata
     7621 cgccggattc attttggcca gcagatcggg cggcagaatg tcctccgtga gacgggacgc
     7681 ggcaataggc gccaccgtgt tcactttaat gttatacttg accccctcga gcttcagggt
     7741 gttcatgaaa ccgacaaggc ccaacttggc cgaggagtag ttgctctggc cgaaatttcc
     7801 gtaaagcccc gcggcggacg tcgtcatgat gatccgtccg taaccgttgt cccgcatgac
     7861 gctgaaagcg gggcgcgtca catggtaggc cccgttcaga tgaacatcaa gaaccgcctg
     7921 ccagttttcc ggctccattt tgagaatgcc cttatcccgc agaatgccgg cattgttgac
     7981 caaaatgtct actttcccaa aggcatccac ggccgacttg acaatgcttt cgcctccgtc
     8041 cggagtcgcc acattggcgt aattcgcaac cgcctctcct cccagagctt tgatctcatc
     8101 aacgactttg tcggccggac cggccgctcc ttcgccggaa ccgtctctgg cgccgccgaa
     8161 atcgttgacg actacttttg cgccccgccg gccaaactca agagcgtaca cacgccccag
     8221 tccgccgcct gcgccggtaa caatggccac ccggccatca aaacggattt ccttgcttgc
     8281 tcccatcatc gttctccttt tcgtaaatgt cttttgaaag caatcgagtt gcgtctttgg
     8341 acaaaagtcc catctccgcc ggaaagttaa aacatgttct tcactcccgc cagcgtgtcc
     8401 tgagcctctt gcaccatcct ctccaacagt tctttgcacg tgggcacatc tttgatgagt
     8461 ccgatggact gccccaccat cagaggggca acctccacat cgccgtcttc ccatgcttct
     8521 ttcatgcgat caccggcaat gagagggtac agagcctcga atcctcctcc tttctcctca
     8581 atttcacata tttcccgaac cacctggttt ttaagagcgc gaccctgcag tttaagcgac
     8641 ttgcaaatca gcgtggtttc gtattcctga cgtttcacaa tctcttcttt gatgttgtcg
     8701 tgaaccacac attccttggt ggcaatgaaa cggctggcca tcatgactcc ctgcgccccc
     8761 agcgaaagag ctgccgcgag ggacttgccg ttggcgatgc cacccaccgt caccaccggg
     8821 attttcacgg attcggcaat acggggcgtc agcaccatgg tcgtgacatc atcgtccagc
     8881 gggtgaccgc cctcttcgat gccggcggcg tagatgccgt cgtatccggc cttctccgcg
     8941 tgcagggcat ggcgcaccgc gcccactttg tgaaaaagct taactcccgc ttttttcaac
     9001 atgtcaatgt actcgggtcc cgccgccttg tccaccggag cgccggacac ttcaatgccc
     9061 gccacttttt cttccgcgca gacccgcagg tacatttgat gatgtcccgg cgtgatgcgc
     9121 acggagggaa ggatggtgag tcccaccata aatggcttgt cggtaagttt gcgcgtctcc
     9181 cggatggcat tgcggaaatc ctcttctgtt tcataatttg ccgcggtgag atttcccatc
     9241 cctcccgcgt tggaaatcgc cgcgcacagc gtcggtttgc aaagccacat cattgccccg
     9301 caaataatgg ggtactggat cccaaacatc tcagttattg ccgttttcat ttgaattctc
     9361 cttatcattt tttaacttgc tgtaacggat tcgtccgctt gccacggcga acgatccgta
     9421 tttttgcctt gtctgacgtc acgcgcaggg atgagacaaa aacatagtat tccccggcag
     9481 gaaatcaacc ttttcgccaa gggaaatccc ctccaatttt ccaaaatatt tcgggaactc
     9541 tgtcgccagc cagaattccg acacgcaggc cttgccgtaa tagaaagccg cctccgcatc
     9601 cctttcaaga atttctgtca gggcctcccc ttttgcgccg gccgtcagcg aggaaagctt
     9661 cggacgcacc agcgccaggc accacagatg catccagccg agcatcaggt cgaagaaggc
     9721 tctccgcacg ggatcaacgt ttaagagcag cgtgtggtag cgcccctcgt caatattctt
     9781 tttcatggtc tctaaagtgg agttcatccg ctcgagtcct gtaaggagca tgttccggta
     9841 aacatcctcg acaatgccgt ttgctttttc gatcgtctgg aatattctcg atttcagcac
     9901 tttgtaattc ttttgatcgg cattgagcag aatctttctc atcaccaggt ccagagactg
     9961 aataccgttt gttccttcga caatgctcag tgatttgcag tccaccgcaa gctgttccac
    10021 gggatactcc gagcaatacc catatcctcc atgaacctga atcgcttcag cggtgatgtg
    10081 ccaggtattt tcagcattgc ccgccttgac cagagggatg agaacatcaa ccagcgaccg
    10141 ggcttcccgg ctttcttctc cttccagcag cttcgcaaga tcaatctgca tggctgcgta
    10201 ataagagagc atacgctgcc cctcgacgag agccttcatt cccatcagca ttcttgtgac
    10261 gtcgggatgt tcaatgatcg gcactttggg agcgttcgtt tcgaacattt tactgaagtg
    10321 aaccccctgc agcctggtgc gcgcataatc cacagcaaat tgtaaagccg cggaggaacc
    10381 tcccagcccc ataatcccga cttcgagtct tgtttcattg agcatctgaa acatgatatt
    10441 catcccgcgg ccccgctcgc ccagcagata ccccgtgcag ttttcatttt caccgaaaga
    10501 catggagcag gttgctgctc cgtgagcgcc catcttgtgc tccaagccgg tgcaaaacac
    10561 atcgtttctt tcccccaggg agccgtcttc attgaccaga tacttgggaa caataaaaat
    10621 ggaaatcccc ttgacgccgg gcgggtctcc ttcaatgcgg gccagaaccg gatagatgat
    10681 ttggcgggtg atatcctggt ctccgttggt aataaaacac ttctgcccgg taattttgta
    10741 ggttccgtcg ggctgcggaa cggcttttgt tttcaaagcc cccacgtcgg atcctgcttc
    10801 cggttccgtg aggcacatgg ttccgcccca ttcccccgaa agaatcttgt gcacatatat
    10861 ggatttctgc cgtgaagtcc cgaaagtatg gattaaatgg gcagccccgt gccccagcat
    10921 gaaaaaaagc atcagcgctc cgcctgcccc acagaagatt tcccacgcgc acgactggat
    10981 ggcgagaggc atccctgccc ctccgatctc cgggtcatcg gcaatgctta aaaaccccgc
    11041 atcgatgatc gctttggccg cggcctgata cccggcaggc agcctgacgg atctggtttg
    11101 cgggtcatag tggcacccct ctttatgccc gttcacgttc gccgggaaga cgcattccga
    11161 cgcaattttt tctgccagcc ggatagtttc ctcataaacg tcacggttaa aatcttcaaa
    11221 acgccggaag cgattcattt tgtccacttc cagaaattcg aacaatgcga attgtaaatc
    11281 acgatggtcc actagatatt tctgcattgc agattctcct ctacagaaaa cgttggttac
    11341 gttttataaa atttttgtac cagtttaaaa acctcgcctg aaaaaaccat gatggtctgg
    11401 ttccggtttt caaggttcac ggccgtctcc agcgagggag catggaggtt ttcattcaga
    11461 acccgcttcg tcagtttcaa tccccccacg cttttacccg tcatggacct tgcgtaggaa
    11521 agcgccgctt ccaacagctc ttcccgcggg accagcttgt tgacgagccc tattctttca
    11581 gcttcctcgg cgccgacctt cctgccggta aaaagaatat cggaggcgcg agcgagtccc
    11641 accagccggg gaagcaaaaa ggaacagccc agttcgccac cggaaagccc gatattaatg
    11701 aaagaggcca caaaataggc ttccttgcac gccacccgga tatcgctggc gagcgtcatg
    11761 gaaaagcctc ccccggcggc cggtccgttc acggccgata taatcggctg gggaatcctt
    11821 ctcatgccca gaatcagggc ggcgtaacgc tcctgcacga gtttcagaaa tttttccgga
    11881 tccgcaaacg cttcggactc cttgtggatc acagcgtcat tgaggtcggc gccggaagag
    11941 taccccttgc cggtacccgt aagaatgaga acccggatgg aatcctcccc ggacaaggcc
    12001 tgaaacagcc tttcgaagtc attgagcata tcgaggttga tggcattgag ctgcccgggg
    12061 cgattcatcg tcactacgcc gatgtgcggc tctttttctt caaaaagcag cgtcttgaat
    12121 tccggcatta acgtactcat gaggtttccc cccttttttc cctgtccata tccctcaatt
    12181 cccgccgcat gatcttgccg atcgcgcttt tgggaagctc agcaatgaat tccacttccg
    12241 taggaacctt ataggccgtc attctctctt tgcagaaggc aactacttcc tgtttcgtca
    12301 gcgtctctcc gggcttggcc acaatgtagg ctttgacggt ttccccccga tacccgtctg
    12361 ggattccgat gcaacaggcc tcgtggatct tgggatgttc aaaaaggatt tcgtcgattt
    12421 ctttggggaa gatattgaag ccgctggcaa cgatgacgtc ttttttgcgg tctgaaatgg
    12481 tcaggtatcc ctcttcgtca aaatacccga tgtctcctgt gtaaaaccat ccatcccgaa
    12541 gggaatttgc cgtttcttcc ggtttgttat agtacccggt catcacctgt ggacctttga
    12601 tacaaatctc accgtgctca ccaacgggga gttctctggt tccggtatcg acatccacga
    12661 ttttaatatc cgtgttggga agcggaagtc ctacggtccc gacttttact ttcccgcccc
    12721 agggggtggt ggtggcaaat gccgtattct cggttgcgcc atagacgtca taaatgatgg
    12781 ccccatggag gtctttcaac tgcttcaggg tgttctccgg caggggcgcc gccccaccaa
    12841 aatatcccct gacataggaa aggttcatct ttcggaattt ttcattgtcc agaagggacg
    12901 tataaatcgt gggcacaccg gtcagaaaag tcggatggaa cttttcaagc atttcgacga
    12961 tcacccccgg ctccggctta ggtatcagcg tacagcccca gccgttccag atgggaaggt
    13021 tctggcttac ggaatatccg gcagaatgaa agacaggata aatacccgtg agattctggt
    13081 attggccctt caggtcggga aaccatacgg agaattgctg gacaaccgac gagatgttgg
    13141 catgcgtcag catggcgccc ttgctgacgc cggtcgttcc cccggtatag atgagcgcgg
    13201 ccagcgaatt ccacttggcc ttgttttcga ccggggtatc cggatacttg gcaatcaaat
    13261 ccatgaattc gtaaacgcca ggatgcggat caaccttgta gaacattgtt tttttaacat
    13321 aggggaaaag ctgcttcttg ggaaacggca ggtagtcgtt gatgtggcag gtgataattt
    13381 tctgaatctt ggtctcgccc ttgattttga gggctcgtgg aagcaacaga tccaatgtca
    13441 ccaggacggt ggcatccgaa tcgtagagct ggtggatcaa ctcccgctcc gtataaagcg
    13501 gattgttcat ggcggtcacg gcccccgccc ggtaagcggc atggtcggca ataattatct
    13561 gcggaatgtt gggcagcagc atcgccacct tgtccccctc ccgcacaccg atgtcgatca
    13621 atgcccttgt gaaccggttg accaatttct cgagctgccg gtaggtgatt tttttaccca
    13681 tgtaaatcat ggcgacccgg tcgggatagg tggcggctgt tctcgtcagg acttccgcca
    13741 tggtgatctt ttctaccgta atttcagctg gaactcctgg agcgtaggat ttgtgccata
    13801 ttctttcaaa agacatatta cccctcctta cttcacttca tgtttgattt accgatatct
    13861 gagcttatcc tgctttgccc atcatcgggt ttttccatca ctcctttgca tctccgtcgt
    13921 gtccttctgc ggcaaattaa accgaagaga ttctatttat tggggtttct atcgtccgtg
    13981 aaatttcggc gtccgtttct ccagaaacgc catgactgct tcaaccatat cgtcgttggg
    14041 gaccaaggcg gcgttgcgct gagccacata ttccaggccc tgccgaatac cgtggtcacg
    14101 gctgtaattg gcgacttctt tgatgccctg aatagcgagg gggggcaaat tggcaatttc
    14161 ttcggccaac gtccgggctt cctcgaagag gacctgaggg gtttcacaaa gtcgggtgat
    14221 gtaacccatc ttcagagatt cctcggccgt ccagtctctg ccggtcaaag ccagctcccg
    14281 aaaccagccc tgcccgataa tgtaaggaac gcgctgaagt gtcccgatat ccgcaatgat
    14341 gccaatgcgt gtttcgcgga tggaaaagat ggcgtctttc gatgccagcc ggatatcaca
    14401 ggcactggta agatcgacgc ctccgccgat acagtgtccg tgaatcgcaa caataacggg
    14461 cttcctgcac ttctctatgg cggacatact ctcctggaga tcatagattt ttcggcgaag
    14521 ttcttcccgg tacacggccg atccgtcccc aagcagcgac tgggccgcca caaggtccaa
    14581 gcctacggta aagctctttc cctccgcccg gatgacaaca acccgcacgt cgttgtcatc
    14641 gtcaaactcc ttgaaaagcc ggggcaactc ctgaaagaat tcaagggtca tcgtgttgcg
    14701 ctgtttcggc ctattcagaa tcagccaggc cacgtggccg gttttttcaa ctttgaacga
    14761 cgattcgttt tccatcctaa tccctccttc tcatggcttt ttcaagcccc ctattctttt
    14821 tcggattccc tgcgcgcctc ctcactccag cctcccatct tttaattcca tcatttgatg
    14881 gattcatatt tgaatatttc cgctttgtca agcaatttgt aaaataaaat atttaaaaaa
    14941 tatcggccac gatccagact cctgtattta ttgccgatta ccgacagact tcggtttgcg
    15001 gccgacaggg gtcttgcgga gcctcgctac tcatataaaa cgacccggaa aaaccatctt
    15061 gacttttcgt gtcatttaca atatcagcac cttcagccgg cggcaaaaag aggcaggcca
    15121 agcaagaccg gagacaccta caatggaaga atatgtacac caaacaattg gagaagaagt
    15181 gcgcttcatc gccgggcatt accagatcgt cgaagaagga cgcctgacgc acgacggaag
    15241 agagtttctc tactggatgg gtattgcgac cgttgacaat gcgtgctgcg gacacaccgg
    15301 ctgccggttt ttgctgatcc ccggttacat tcattcatgg aaaaccaaaa caaatgccca
    15361 gggaaagcca gtcagcgaag tgaaaccgat cgtcgatgaa cgtgaacggg cggccatccg
    15421 aacatttctg gaggcttgct acccttatgc gcagatcaat ttttccataa aatcacagct
    15481 cgtgtaaaat ctgtacggcg gcaattcaaa ggatgtccaa agcgacaaac cactctgatt
    15541 tatcttcggc cacatcctga gatcattccc gatattgagc ccagatcaga aattgtcttg
    15601 acgacggggg aggtaatgtc gtaggtcatc aaccaaatta cgtattcaat caaataatgg
    15661 agccaaaaaa tgaaatcgcc ccttgaaaaa gcccgtaaaa accctaaatc catgaaggca
    15721 aagatccttc acgccgcccg ccgtattttt ggcgaatacg gcttccacgg gacgacaacc
    15781 cgcatgattg ctaaggaagt gggaatcgac atctccaccc ttcactacca ctggggcgat
    15841 aaaaacgacc tgtatgaagc cgtggtcgtg gacgtgaaca acgacctggg cagagaactg
    15901 cgcctagtgg aaaatatcgt ccacggcagc tctcttgaaa aacggctgaa ccgctccatc
    15961 gaccatatga tggattatct gtttgaccat ccggagatat cgaatcttat tctgcttcgc
    16021 tacttcagca aaacacgcaa cgaagcgaac gtagatctca aggtgccgga attcagcggc
    16081 gatatcgccc gcgccatgga tctgacgaaa gacgcaaaaa atccaccgcc aagggctatg
    16141 atggaagtgc tgggcatcat gaacggaata cacagctttg tctccggaga aaattttttc
    16201 cgtcccatgt tgaatcttaa tcgggaaaat tacatccgtg ccgtcaagga aaccctgaaa
    16261 tttttactcg ttcccgcatt tgtcctccgc gaaaagcaaa caaaaaaagc gaagaacggt
    16321 tcattatcca aaaagactga agaaaaaaag aaaaaccaaa tcaagaaatc aaaataatga
    16381 aatgaataca aaagagcgct ttcagatcat tcctgctcct gagcaaaaag gtcagggcgg
    16441 gattcatcaa ggatctggaa gtattccatg aaggtcattt caaaatatca tgcaggatcg
    16501 taccgccccc taatcaatat agcttctgct ggcagggagg agaaccggct tccaactctg
    16561 acgaacaacg cccggcgatc tgcctttgcc cgtctcaagt ccgactggca ggaggaatgc
    16621 aaagaccgct ccattgtttc gtttcgtatg ggggactgca caaggtttcg attccgcaga
    16681 gattcggggt atcaaaatgt caatgaatat tgcaccgccg tgaaaacggt ttatcgccac
    16741 attaagcggc atcttttccg gcccggagag ggacggaggc aaaggccgac cctgctccgg
    16801 accgccgggg ttcatggatc atcatcagtt aaaaatcatg aaattcccct ccctcatggg
    16861 tgcccttctc tttgtcgcca aaggggatca gacgctccgc ctccgtcctc ttccggggaa
    16921 ccgggatatt cttcctggtt gtcgttgcgg ccgtccggtc ggggggaacg acggcgcccc
    16981 gtcgtctatt caaagccgct accattcccc ctccgccttt ttcttcgccg ccttccacga
    17041 cttcgaggag tctttcggcc acatgtctga tctgctcggc ctgggcgttc atctcttccg
    17101 cggcactggc cgtttcctcc gcgttggcgg cgctctgctg gacaaccttg tccatctcca
    17161 cgaccgccga attgatctgg gtgataccct gggcttgctc gcgggacgcc gccgcaatct
    17221 ctccgctcag ttccgtcacc ttcttcgcga tgtcactgtt tttagccatc gcttcggaaa
    17281 cctgtccgta aaaagaggtt gcctccttga tccggcccgt tgaattgccg atcagatcct
    17341 gggtattctt ggccgcttcc gtggcccgca tggccagatt cctgacttca tcggcgacga
    17401 cggcaaatcc ggcgccggcc tcaccggccc gggccgcctc cacggcggcg ttcaaggcca
    17461 gcaggttggt ctggaacgca atttcgtcaa tggtcttaat gaccttcgcc gtttcctcgc
    17521 tggcgctgac ggaggcctgc atggcttttt ccatgatctc cagccgctcc ccgatcatct
    17581 ggaaattggg ggccgcctcc ttcaacatca tgtcgttggc cagttgcgcg ctattcgcgt
    17641 tctgacgggt catggaggat atctcctcca aagaggaaga ggtctcctcc accgccgctg
    17701 cctgttccga cgccccttcc gccaaggatt ggctggcgga tgagacctcc gaggacgccg
    17761 aggtcaactg cgccgccgca tcgttgagtt cgttggccac cctggcgatg ggaacgctga
    17821 tccggcgggc aaagacaatg atggcaaaga cggccacgcc aaggaagatc acgctgatga
    17881 tcgcgatgac gttgcggatc ccatgggcgg ccgccaggaa ttcatcggta ttctgagtga
    17941 aacccacgct ccagccggtg atttccacgg gggcgaagcc gcagattttt ttgtccccct
    18001 tgaacacata ggattctgat cccttctgtt ttcccgtcat cttgccgaca atctccgtca
    18061 tctccgcctc tttcgtaagg tccagcgtca ggatgtattc tttcttcgga tgggcgatga
    18121 ccattccggc ccggtcgatc atgaagggat agccggtctt ccccaccttg gtgtcggtga
    18181 ttctttccac gaggaaatcg accttcatgg ccgcggcggc aaccccgaga aattccccgc
    18241 cctgactgta aataggggcc gaaacactgg aaacggtgtt ccccgtagcc ttggatttgg
    18301 tcacattgcc tacgaaaacc tcgccgcctt tccccgccag atagtaatcc cgcgccgata
    18361 catcgagccc cttgagcttt ccccccacat catcgcagaa tattttgcca tttttgtcga
    18421 ccagatacaa ctgatcataa tcattgccta tcttcttcat aatctccgta agggtcgcgt
    18481 tcatcgcttc cagctccgac accggcacgg cccctgttgc ctgcttgtcg gccacggcca
    18541 cgaccgccgg ataggcggat aattgcttca caatcttcaa ttcttctaat aataccatct
    18601 gcgccatctc ggcaagactg ctggcaatct gctccgactc ttcccgggcg ttctgctgca
    18661 gggcttggga ggacctgacc gcggaaaaga cccccaccac cagcaacgga atgaggacca
    18721 acaacactcc gcctacgagc agcttcatgt tcaaattcat cttcttcata ctcttcatac
    18781 ctccctattg attttgatta agaacttgct ttctgcgaaa ggtatttggc agcatgtgta
    18841 ctattgagaa aacttagcac ctttatgatc tgttctgatg ccaggttaac tgcaattcag
    18901 acaaaccttt ctgtcacgtc ctgtttctgt attcatccgg gcgtcagttt gcgcatgtgg
    18961 aagatgagcg agataatcag cagctgggaa caagatcctc acgggcttct ttcttctgtt
    19021 tactccgcga gacgttgctg taccagccgt aatatctcac catctgctcg acccggttcg
    19081 ggatatggat gcacatggcg accatccccc ttgtgatctt accctccttc agaagtaact
    19141 tgaaaacctt gtgccgaaaa tcatctccag ctttttcagc tccaaggacg gggcgatggg
    19201 aacagaagtc gaggttcgga aattaacgcc tctacaggtt gaaaaatctc ttcatcagtt
    19261 cccgaacgtg aactttttct tccgcgtccc cttcgagttt ctcgacaatc agcggcagcc
    19321 acttatccca gttggccgcg tcgaaatgca tgccgatctc ggcgtaaagc tcttcggaag
    19381 cttcgacata atttaaaagc ccccagagaa cttcaagtct tacttcatag tcgcatggtt
    19441 tgtcccacag agcgcggagg tattctttta catcctgatc acccacaaat tgctgcagca
    19501 gtttcagcgc gaaacgcctc ataggaagtt cgtactgccg ggaagaagca atttccatca
    19561 gaatcttctt atcttccgca gtaactgcct gccggcaata gccgaaaaga ggataggtgt
    19621 ttagaagctg ctgcatggct gtttccggcg gcgtgccgtc cctgaggtcc tg
//
LOCUS       DAOSIM010000014        18687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_3196_length_18687_cov_7.935487, whole genome shotgun sequence.
ACCESSION   DAOSIM010000014 DAOSIM010000000
VERSION     DAOSIM010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 18687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18687
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3196_length_18687_cov_7.935487"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PLR47_01390"
     CDS             complement(<1..432)
                     /locus_tag="PLR47_01390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG11617.1"
                     /translation="MPEAKKTGGFYGWVMMPLLCLVYSIPIGFALYGPPVIYRFMQKD
                     LSWQRGEINLGYTIIGMMLGLGAFIIPWLMDRFGPRKTLAIGASLTAGSVLLMAAAGQ
                     SYPVYLALCVCTGLGISFGSVVPVQALVLLWFNVHRALVMGL"
     gene            660..1175
                     /locus_tag="PLR47_01395"
     CDS             660..1175
                     /locus_tag="PLR47_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11618.1"
                     /translation="MKNTKPIKVKNCFIREAGNPKGEGGCARIVSFFLALLVLFLPAA
                     PAAAQWIEYLRDEDGTVFSYHKKLNIDKKNEKHIVQVLGKENISDAGREKYLQSLKQK
                     GLPAAAYKQLSRKMFLCEIDCREKRIRKASLTAYDTNGKVIFSGENPSPEWEPILSDS
                     TGEALQKSVCK"
     gene            1342..1752
                     /locus_tag="PLR47_01400"
     CDS             1342..1752
                     /locus_tag="PLR47_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOG11619.1"
                     /translation="MISYQDVILFILSKANQKVYGTFKSRTQLYGLTPIQGLVLHALY
                     EEEGLSAGELGKRLSLDSATLSGVLDRMAESGWIIKNVKDDRRVLNIRLTDKALECRD
                     KFINDTEDLNREIFSRYTLEERLLLIRMLKDLRR"
     gene            1807..3309
                     /locus_tag="PLR47_01405"
     CDS             1807..3309
                     /locus_tag="PLR47_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOG11620.1"
                     /translation="MSSKTRRTGKTGKAHYPPTDYERIFHPKAIAILGVSADENGVGF
                     GTGMLRAIMAMGYEGTIYPVNPKGGTIYGLEIHQRVEDIPGPVDFAVVAVNARYVPES
                     LEACLKIGVAGAEILSSGFSELGTEEGKELERRIQDIAARGIRVVGPNCFGIYCPASG
                     LTLLPGPDLSRETGPVAFVAQSGGMSIDFAHAGKWLGVRFSKMISFGNGADLREAELL
                     RYLADDAETRVISMYIEGIKDGERFFSELKAAAAKKPVIVYKGGLSVAGARSVASHTA
                     SMGGSRVIWQSILKQAGAIQVNDTEEMAQANLAFAMLPPKPFRGISIVGGGGAIGVTA
                     CDTAETFGIEIPALPPQLQERIGSVLPKPGSSAINPIDVANPFVGPQALKEVLLGAAE
                     DERIELQIIVSLLFHYKGLARAFGRPVAEVTPYRELAQCVRDAIDRTGKPVIAVLPNM
                     TRGVESMDITELIALTRQEYLSRGIPVFDELHDAIRAISHINNYYGGKIK"
     gene            3306..3992
                     /locus_tag="PLR47_01410"
     CDS             3306..3992
                     /locus_tag="PLR47_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOG11621.1"
                     /translation="MKNEKAAKIIDKALKQGRKTLSEYEAKQVLAAYGIPVTKEVLAR
                     DKTALEKGLKKIGFPLVMKGCSADIAHKTEKGLIHVDIRTATEAKKAFREILAGMEGY
                     EGGVLMQEMIKGRRELVMGLTRDPQFGPCVMFGLGGIFTEILRDVAFRRAPLEIRDAK
                     EMMQEIKGRKILDAVRGMEAADQKMLVDMLISVGRIGLDFDDIMEIDLNPVILSGSKP
                     VVVDALIVLK"
     assembly_gap    4168..4267
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4268..4507
                     /locus_tag="PLR47_01415"
     CDS             <4268..4507
                     /locus_tag="PLR47_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11622.1"
                     /translation="GFGGPNNLKATSGLPYVVMVSLSNHNERYELQKRKEPENLRMHL
                     RTLQPQGNLLRLRSLSPAEKTGAGLLFSAGRRKNL"
     gene            4714..5883
                     /locus_tag="PLR47_01420"
     CDS             4714..5883
                     /locus_tag="PLR47_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HOG11623.1"
                     /translation="MTTPRNIDGQDGYQVSFQKENESLLISLKGEFGLKNLTAFTREI
                     RQAVSGHAIPTLTIDFSQVKYLDSAAALAFVQMEQEAASRKTNCHLANLSEEARGIFS
                     VIHPDVLTRRPFKPEHTGEGVVQQIGQASLSIGRDFVNFITFIGDLLSALAYTVTHPA
                     TLRGKDVLFYVQRAGVDGLPIVILIGALMGLIMAFMSYLQFKQFGANIYVPTLVSFAM
                     IKELGPIMTAILVAGRSGSAFAAEIGTMQVNEEVDALETMGFDPVRFLAVPKVLAAVI
                     VVPILTVYADVSGILGGLIIGVTTLDLTFKTYISQSIKTIQVFDVVTSLIKAAVFAGL
                     ISAIGCQRGFQVRAGAQDVGKFTTSAVVAAIFLIVLTDSIFAVMLYYVKRYSAFA"
     gene            5903..6700
                     /locus_tag="PLR47_01425"
     CDS             5903..6700
                     /locus_tag="PLR47_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG11624.1"
                     /translation="MTEPSEKKTVLSVKNLTQRFDDNIVFEDVSFDVYQGEVLVIVGG
                     SGCGKSTLLKIMIGLQKPYAGQVLYQGTDITRASDEALNEYRKNIGVLFQSGALFSSM
                     SIKENIALPLQEYTDLDADTIDRVIRMKLGMVNLAGYENHNPSELSGGMKKRAGIARA
                     MALDPRVLFLDELSAGLDPITAVELDDLIIKTNEALGTTMVIVTHELESIYKIAHRVL
                     MLDKEARGMIAEGRPLELKENATDPRVRSFFLRQLPENQDEQRFYAD"
     gene            6690..7622
                     /locus_tag="PLR47_01430"
     CDS             6690..7622
                     /locus_tag="PLR47_01430"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOG11625.1"
                     /translation="MPTRSSKFSIGLFVIVGALICAGVIIWVGAAKFFTKGSFYNVYF
                     DESVQGLQVDSAIKYRGVEIGKVQKIDVAPDYRLIEVTMKIDLEGDLQKQTIATLKTA
                     GITGIVFIELDRIKPGELADSPKLTFKADYPVIPSRRSEISRLIGDTNMIMQNIKKID
                     FEGISSQLKNTAQAIENFVEGKRITSLMTHLENTSANLDETIAGINKTVAEGKVDRAV
                     TEALGVLTDARKLVGRAREEIGALNLREKSAQTDAILQNIDKKTKIITTGLADTSENL
                     RVTSENLQRLAESLNRDPSDLIYTKPAPPRKPME"
     gene            7638..8306
                     /locus_tag="PLR47_01435"
     CDS             7638..8306
                     /locus_tag="PLR47_01435"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HOG11626.1"
                     /translation="MEKSLQRQARFHKPSSVMPLFVIIALLLCAAGCAGGGKPQYDVQ
                     KYLLAYPSPTWDGSAKLPVSVKVNRFSIAAAYNSTGMIFRDDAYGLDAFNYSRWAVNP
                     ADMIGDLLLADMRAGNLFQAVLSRYDLEEGRFILSGGIEEFYLQTEKKNRAARIVIAI
                     SLQDSREKETGKKMMYQKTYTREAPLQEASPKGYCAAASQAMQALSREIIGDVYSAVR
                     ARTP"
     gene            8322..8714
                     /locus_tag="PLR47_01440"
     CDS             8322..8714
                     /locus_tag="PLR47_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOG11627.1"
                     /translation="MKEISVRSSSRFEMIDITEAVRKTAREEKIESGIVLVYTPHTTA
                     AVTINENADPDVPRDLLAALDRAVPLSANYRHTEGNSAAHVKSSMVGASEMVIIENGR
                     LVLGTWQSIFFCEFDGPRSRKVIVKFLT"
     gene            complement(8861..9664)
                     /locus_tag="PLR47_01445"
     CDS             complement(8861..9664)
                     /locus_tag="PLR47_01445"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOG11628.1"
                     /translation="MRELTDFAVYLARKAGRMLAEKFTRHNPVYYKGAIDLVTEADRM
                     SEEIILAEIGRRYPGHGILSEESKEKNARAAMRWIVDPLDGTTNYAHGFPFFCVSVAL
                     EKDGSVVLGVVYDPLRDDLFEASLGGGSRLNGKELRVSSVEDLSRSLLATGFPYDIRE
                     SPDNNLNFFNAMAKKAQAIRRAGAAALDLAYLAAGRFDGFWELKLKPWDTAAGCLLVE
                     EAGGVLSDMAGEKWHLFSPGLVASNGLIQEQMLEVMSAVVNGGKTGEGQ"
     gene            9804..10700
                     /locus_tag="PLR47_01450"
     CDS             9804..10700
                     /locus_tag="PLR47_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HOG11629.1"
                     /translation="MTTEKNADSPIPDLKTARQNSGQTLKEMFERTRISVVNLEAIEN
                     GQFHLLPVPTYTRNFIKIYAQALGVDSSPLLGRYEEYLQALKNKEKEQAAVKPPPAPL
                     AEMLSRYKIFLWITLIVILIAAVSIFASLYNKPLSESQQGIQRTGEAEGRAAPAQVAA
                     GADALPVGIGLPKPEEIAGVQPPESQQVMASPSIPSSAVSQTPAAQKAPEKDKAAQAA
                     AGAEETSLLIIRATEQTWIRMQVDDKEPYEVLLEAGETLSQKASRFNMDVGNAGGIRI
                     QLDGKTIENLGKSGQVIHLRLP"
     gene            10727..10906
                     /gene="tatA"
                     /locus_tag="PLR47_01455"
     CDS             10727..10906
                     /gene="tatA"
                     /locus_tag="PLR47_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOG11630.1"
                     /translation="MFGIGMPELLIILVIILIIFGVGKLPEIGRGLGEGIRNFKKSVS
                     DKNDANPPAKKEGDK"
     gene            complement(10948..11355)
                     /locus_tag="PLR47_01460"
     CDS             complement(10948..11355)
                     /locus_tag="PLR47_01460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOG11631.1"
                     /translation="MVNKALLIGRLGRDPEVRYTPDGTMVTNFTMATDETYKDKNGER
                     VQKTEWHRIVAFGKLAEICGNYLVKGKLVFIEGRIQTRAWEDKEGVKRNTTEIIASNM
                     QMLDSKGQAKSGDATPEAPSAQNSGTPMDDVPF"
     gene            11526..13007
                     /gene="lysS"
                     /locus_tag="PLR47_01465"
     CDS             11526..13007
                     /gene="lysS"
                     /locus_tag="PLR47_01465"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOG11632.1"
                     /translation="MEDNDLLKVRLEKIAALKAAGTDLYPNDAKPQNTTDRILQEYGG
                     MDSDGLAKLTQKFSLAGRLMAIRSFGKAAFVKIQDRKGQLQCYIAKNILSEQDFSIFK
                     KLDIGDIIYVSGKLFRTKTNELTLEAESVRLTAKSIRPLPEKWHGLTDIETRYRQRHL
                     DLISNPAVKEIFIRRSLIIRLIRQFMDRHDFLEVETPMMQPKAGGAVARPFKTHHNAL
                     DQDLYLRIAPELYLKRLVTGGLERVYEINRNFRNEGISTFHNPEFTMMEFYASYATYE
                     DLMSFTEELFAFIAREIFGKLAFTYQGTEIDLTPPWRRLAVKDALVEIAGMTPDEMAD
                     PAKAIDFARNAGCDIKETDPPGKILMAIFDERVEKKLIQPTFVTHYPVAVSPLSRRSN
                     TDPDLVDRFELYISGREIANAFSELNDPADQRERFAAQLKEREAGDDEAHEMDEDYIR
                     TLEYGMPPTAGEGIGIDRLIMLLTDSASIRDVILFPLLRSRTE"
     gene            13069..14355
                     /locus_tag="PLR47_01470"
     CDS             13069..14355
                     /locus_tag="PLR47_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HOG11633.1"
                     /translation="MSYEFFISRRYMRAKRKQVFVSIITFISIFGIFLGVAALIIVLA
                     VMNGFEEDLRSKILGIRSHIEVTADMSGPMKNYPAVRREIEQIPGVVASTPFIYTEAM
                     IQSPNGVTGVVIRGMETQSAPRVINLGKIRQGSVDHLDGIPEAVRQKLPAADRELPGI
                     LIGREMARNMGIFLHDPITIISPAGGIATPMGMVPRMKKFVVTGIYESGFYEYDSKLA
                     FLSLASCQDFLEMGDAVSGIDIVVRDIYQADVIARKIQSRLGFPFYAQNWMQMNKNLF
                     SALRLEKRVMFIILSLIVLVAAFNIISALIMIVMEKNKDIAILKSMGATSATIMKIFI
                     YQGLIVGMVGTFFGSVAGLAIALNLQKISLLVEKVFNFKILPGDVYYLSELPSKVNYG
                     DVALIVAGTLAICFLSTIYPSWRASRLDPAEALRYE"
     assembly_gap    14501..14510
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14511..14973
                     /locus_tag="PLR47_01475"
     CDS             <14511..14973
                     /locus_tag="PLR47_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG11634.1"
                     /translation="FNWNEKKIAAFRNRTIGFVFQFNNLLPEFTALENAMMPALIGGL
                     NKKEANDRAARLLTEVGLEHRLQHKPGELSGGEQQRVAIARALVMEPEILLADEPTGN
                     LDTETGKKIEDILIDLNAEKKITLVVVTHNRLLAERMSGRIGLRDGKIYDF"
     gene            14963..17608
                     /gene="bamA"
                     /locus_tag="PLR47_01480"
     CDS             14963..17608
                     /gene="bamA"
                     /locus_tag="PLR47_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOG11635.1"
                     /translation="MIFNFKTVRLFIAVVLLLFAMEARADAPKKICILPFEVHASTGS
                     AELQASVHNSLLTEFRREKKVEVIAAGAFEPGGPAPGEAEAGAAGKELGADYVVFGSV
                     MRFGETLNIDAQIIDVAGEKILSAVSVQGKETEGAGALAGALKREILGRAGLTDKIAR
                     VEIAGNRKIGADAVVQKLKSRPGKVLDEDDIRDDIKTIFKMGYFLDVSADVTAEAGGK
                     VLTFNVVEKGLISEIRLIGNKKLDRDDILEAMSVKVRQSVNPENVKQDLQKIKALYDA
                     KGYYNAEISDRLEKDGEKDFVLILDIRENDRVYIRSITFEGNESFTAKELAGIMTTNE
                     RTLLGFITDTGILQPQQLKQDVQRLTAFYFNNGFVNAQVGEPEIRHDDKGIYIHFTVR
                     EGKRFKMGKVEISGDYLEKTRDDLYASLKTKAGSHYSREAIMKDIEMITTTANDEGYA
                     NADVYPQVATHEKEQLVDLNFVVKKGELIHIARIGISGNTTTRDKVIRRSLFIAEGDL
                     YSSSRLKKSYENLNQLRYFEEVDIQTEKAPDNQMDINIRVKEKNTGMFMIGAGYSAVD
                     QAVIMAQIVQQNFLGYGQTMSLKASLGSTTNNYELSFTEPYLFDLPLWFKADVWKYKK
                     EYDSYSLDTKGVGVTVGHPLFWKIVGYVGYKFSMDNIQDINAATASTYIKAQAGERTS
                     SALSFTMVYDTTNDVMFPSKGIKASASVQHAGMPLGGNTNFTKYATTATGFVPLFWDM
                     VFSLKGQIGYLQNNDDSDDKLPIYERYVLGGINSLRGLRYVGPTNPGTSDVIGGTTML
                     TMTAEIVFPLIKNAGMKGVVFYDTGNTWNGGYHLNDLRSTCGAGIRWYSPIGPLRLEY
                     GYVLDNTGLNDDSVGRWEFTIGMFM"
     gene            17675..18205
                     /locus_tag="PLR47_01485"
     CDS             17675..18205
                     /locus_tag="PLR47_01485"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOG11636.1"
                     /translation="MKKNMLWIAGLMLLLFAWTNTSLAADRIGFINMQTIIQNSNAGK
                     KAAEDFKKLFARKQESIKTMENEVKKLKDELDKQGAVMTAGVRGDKEAAYQRKMRDYQ
                     ILVDDTNKELQKRDQEYSQQLIPEILKVVRTIAEKEKYTLILDVSTMPLPYFDKAGDI
                     SRKVIEDFNKTQVGKK"
     gene            18220..>18687
                     /locus_tag="PLR47_01490"
     CDS             18220..>18687
                     /locus_tag="PLR47_01490"
                     /inference="COORDINATES: protein motif:HMM:NF016494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HOG11637.1"
                     /translation="MKKSIAEIAALVGGMVTGDDRKRISDIRPIEEAGADDLSFVANP
                     KYFKWLKTTLAGAILVPPGTVAPDKNLIAVADPYAAFGKLLTVFYPVDHGPAGISPDA
                     FIEEGAFVSPEATVMPRAFIGRGARVEKGAVIYPGVFIGRDASVGEDSVLYANV"
BASE COUNT         4549 a         5219 c         5116 g         3693 t
ORIGIN      
        1 caggcccatg accagcgccc ggtgaacatt aaaccacagc agaaccagcg cctgaacggg
       61 cacgaccgac ccgaaggaaa tccccaggcc ggtgcagacg caaagcgcca ggtaaacggg
      121 ataggattgc cccgcagccg ccatcagcag aaccgatccc gccgtcagag acgcgccgat
      181 ggccagcgtt ttgcgggggc cgaagcggtc catcagccac ggaatgatga acgcgcccag
      241 accaagcatc attccgataa tcgtatagcc gaggttgatc tctccgcgct gccaggacag
      301 gtccttctgc atgaaccggt aaatgacggg cggcccatag agcgcaaagc cgatcgggat
      361 cgaatagacg aggcacagaa gcggcatcat cacccaaccg tagaaaccgc cggttttttt
      421 cgcttcaggc attgcgaggc cctcccgaac attcggcagc ctcgttttcg ctttctttac
      481 gccccgattt gaccattcct tgctccggat tcgtttgttt cagcgtaacc gactcttttt
      541 gcgttggcca tctttttaaa gaacagccct ttgcctaaac ccgagcgaaa gagccatgaa
      601 aattcgatgc aagatttcct gttgattcct gatgtgcttt tccgtataca ggaaacgcta
      661 tgaaaaacac aaagccgatc aaagtcaaga actgtttcat ccgggaagcc ggaaacccga
      721 agggcgaagg aggatgcgca aggatcgtct ctttcttcct ggcgcttctg gtgctgtttc
      781 tgccggcggc gcccgcggcc gcgcaatgga tcgaatacct tcgggatgaa gacggaactg
      841 ttttttccta tcacaaaaag ctcaacattg acaaaaagaa cgaaaaacac atcgtccagg
      901 ttctgggaaa agaaaacatt tccgatgcag gcagggaaaa atatcttcaa tccctgaagc
      961 aaaagggatt gccggcggcg gcatacaagc agctgtccag aaaaatgttc ttgtgcgaaa
     1021 tcgactgccg ggaaaaacgg attcgaaaag cttcgttgac cgcctatgac acgaacggca
     1081 aagtgatctt ttccggggaa aatccctccc cggaatggga gcccatcctt tccgattcaa
     1141 cgggagaagc actccaaaaa tccgtctgca aatgaattcc cttcaccccg gaatcaacag
     1201 gcccaaaact ctgggggcgg gaagtacggg gatttcttcg gccaatcaac tttttaccgg
     1261 tcaacgccgg tcaacatttt tcttgacttg acctctccct ttatttatct tgcacactaa
     1321 ataatgaccc gggtgtcacc gatgatcagt taccaggacg tgattctgtt tatcttaagc
     1381 aaggccaatc agaaagtgta cggaactttc aagtcccgca cgcagttgta cggccttaca
     1441 cccattcagg gactcgtcct gcacgctctt tacgaggaag aagggctttc cgccggtgaa
     1501 ctgggaaagc gattgtccct ggacagcgcc acgctgtccg gcgttctgga ccgaatggcg
     1561 gaaagcggct ggatcatcaa gaacgtgaaa gacgaccggc gggttttgaa catccggctg
     1621 acggataagg cgctggagtg ccgtgacaaa tttatcaatg ataccgaaga tttgaaccgg
     1681 gaaattttct ccagatacac cctggaggaa agactgcttt tgatccggat gctcaaggac
     1741 ctgcgcaggt gacgatccct gttgctgttt agcatacaag gtaaaatcgg caagcatgga
     1801 ggcagaatgt ccagcaaaac cagaagaaca ggaaaaaccg gaaaagcaca ttacccgccg
     1861 accgactacg aaaggatttt tcatccgaag gcgattgcca ttctgggcgt ctccgcggac
     1921 gaaaacggcg tcggcttcgg aaccggcatg cttcgcgcca tcatggcaat gggatacgaa
     1981 gggaccatat accccgtcaa cccgaaggga ggaacgattt acgggctgga aattcaccaa
     2041 agggtggagg atattcccgg cccggttgat tttgccgttg tcgcggtgaa cgcgcggtat
     2101 gttccggaat ccctggaagc gtgcctgaag atcggcgtgg ccggcgcgga aattctctcc
     2161 tccggcttca gcgaactggg caccgaagag ggaaaagaac tggagcggcg gattcaggat
     2221 atcgccgcac ggggcattcg cgtggtgggg cccaactgct tcggcatcta ctgtccggcc
     2281 agcggcctca cgcttttgcc ggggccggat ctgtcgcgtg aaaccggacc ggtggcgttt
     2341 gtcgcgcaaa gcggcggcat gtcgatcgat ttcgcgcatg cgggaaaatg gctgggagtc
     2401 cgcttcagca aaatgatcag cttcggcaac ggcgcggatt tgcgcgaagc ggagcttctg
     2461 cgctatctgg ccgatgatgc ggaaacccgg gtcatctcga tgtacattga aggcatcaag
     2521 gacggagagc gttttttctc cgagcttaaa gccgccgcgg ccaaaaaacc ggtcatcgtt
     2581 tacaaaggcg gcctttccgt ggccggcgcc cgcagcgtgg ccagccacac cgcgtcgatg
     2641 ggcggaagcc gcgtcatctg gcagtcaatc ttaaagcagg cgggagccat tcaggtaaac
     2701 gatacggaag agatggcgca ggccaacctg gcctttgcga tgctgccgcc gaaacccttt
     2761 cgggggattt ccatcgtagg cggcggaggc gcaatcggcg tgaccgcctg cgacacggcc
     2821 gaaacgttcg gcatcgagat tccggcgctg ccgccgcagc tccaggagcg catcggaagc
     2881 gttttgccca agcccggctc gagcgccatc aatccgattg acgtcgccaa tccttttgtc
     2941 ggaccgcagg cgctgaagga agtgcttctc ggcgcggccg aagatgagcg gatcgaactg
     3001 caaatcatcg tatcgctcct gtttcactac aagggtcttg cccgcgcctt cggcaggccc
     3061 gtcgcggaag tcaccccata ccgggagctg gcccagtgcg tccgcgacgc gattgaccgg
     3121 accggcaaac cggtgattgc cgtgctgccc aacatgaccc gtggcgtgga aagcatggat
     3181 attacggagc tgatcgccct gacgcggcag gaataccttt cccgcggcat tccggttttc
     3241 gacgagctgc acgacgccat tcgggcgatt tcccacatca acaattatta tggaggaaaa
     3301 ataaaatgaa aaacgaaaaa gccgcgaaga tcatcgacaa ggccctgaag cagggacgca
     3361 aaacgctttc ggaatacgaa gccaagcagg ttctggccgc gtacggcatt ccggtcacga
     3421 aagaggttct ggccagggac aaaaccgcgc tggagaaagg cctgaaaaaa atcggcttcc
     3481 cgctggtcat gaagggctgc tcggccgaca tcgcccacaa aacggaaaaa ggcctgattc
     3541 atgtggacat ccgcacggca acggaggcaa agaaagcgtt ccgggaaatt ctcgccggca
     3601 tggaaggcta tgaaggcggc gtgctgatgc aggaaatgat caaaggcagg cgtgaactgg
     3661 tcatgggcct gacccgcgat ccgcagttcg gaccctgcgt gatgttcggc ctgggcggca
     3721 tttttacgga aattctccgc gacgtcgctt tccgccgcgc gcctctggaa atccgggacg
     3781 cgaaagagat gatgcaggaa atcaaaggcc gcaagattct cgacgcggtg cgcggcatgg
     3841 aagcggcgga ccagaaaatg cttgtcgata tgctcatcag cgtcggccgc atcggcctgg
     3901 actttgacga catcatggaa atcgacctca acccggtgat tctttccgga tcaaagccgg
     3961 tggtcgtgga cgcgctgatc gttttgaaat agactgaagg ctaaagaccg aaggctgaag
     4021 gcggaagaac aaattaaata gagacagcgc ctctttattt ttgctttatg tagggcgcac
     4081 ctttaggtgc gccgcacgtt gattgacatt ccggttttgg cgggcctaat aacctaaagg
     4141 ccacaagcgg cctgccctac gttgtcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnggt tttggcgggc ctaataacct aaaggccaca agcggcctgc cctacgttgt
     4321 catggtgagc ctgtcgaacc ataatgaacg ttatgaactg caaaaaagaa aggaacctga
     4381 aaatctgcgg atgcacctac gaaccctgca gccgcaaggg aatctgctgc gactgcgtag
     4441 cctatcacct gcagaaaaga caggtgccgg gctgcttttt tccgcaggac gccgaaagaa
     4501 cctatgaccg ctcctttgag aattttgcgg acctggtcgc aaaaaaacgc atatgatccg
     4561 gcggcgcgca tccgccgcgg acaagccttc gcgcaaacgt cgatcccttc gcaacgacat
     4621 tgattttcat tgacaagcac cggtcaaacg gcttaaatac gcctgcaaac acgtcatttc
     4681 caatatcgaa ttcggggcgg gaaaaacatt gccatgacga ctccgcgaaa cattgacggt
     4741 caggacggtt atcaggtttc ttttcaaaag gagaacgaaa gccttctcat cagcctgaaa
     4801 ggcgagttcg gcctgaaaaa tctcacggcc ttcacccgcg aaatccggca ggctgtttcc
     4861 ggacatgcga ttccaaccct gaccatcgat ttttcacagg tcaaatacct ggacagcgcg
     4921 gcggctctgg cttttgttca gatggagcag gaagcggcaa gccggaagac aaactgccat
     4981 ctggccaacc tgagcgaaga agccaggggc attttcagcg tgatccaccc cgatgttctg
     5041 acccggcgcc ctttcaagcc ggaacacacc ggcgagggag tggtgcagca gatcggacag
     5101 gcctctttgt cgatcggccg ggattttgtg aatttcataa cctttatcgg cgacttgctc
     5161 tccgcgctgg cctataccgt gacgcatccc gcgacccttc ggggaaagga tgttcttttt
     5221 tacgtgcagc gcgcgggtgt ggacggtctg ccgattgtca tcctgatcgg cgcgctgatg
     5281 ggtctgatca tggccttcat gtcttatctg cagttcaagc agttcggcgc gaatatttat
     5341 gttcccacac tggtcagctt cgccatgatc aaggaactgg ggccgatcat gacggcgatc
     5401 ctggtcgcgg gacggtccgg ctccgccttt gccgcggaaa tcggcaccat gcaggtcaat
     5461 gaagaggtgg acgcgcttga aacgatggga tttgacccgg tccgttttct ggccgtgccg
     5521 aaggttctgg ccgccgtgat cgtcgtgccg attctgaccg tttacgcgga tgtctccggc
     5581 attctgggcg gcctgatcat cggcgtcacg accctggacc tgactttcaa aacctatatc
     5641 tcgcagtcga ttaaaaccat tcaggtgttt gatgtggtca ccagcctgat caaggccgcg
     5701 gtatttgccg gactcatttc cgccatcggc tgccagcgcg gctttcaggt gcgcgccggc
     5761 gcccaggatg tcggcaagtt tacgacctcc gccgtggtcg cggccatctt cctgatcgtg
     5821 ctgaccgact ccatttttgc cgttatgctc tactatgtca aacgatacag tgctttcgca
     5881 taaggaagga accgggattt ccatgacgga accatcggaa aaaaagaccg ttctttcggt
     5941 aaaaaacctg acgcagcgct tcgacgacaa catcgtcttt gaagacgtga gcttcgatgt
     6001 gtatcaggga gaggttctgg tcatcgtggg cggcagcggc tgcggcaaat caacgctgct
     6061 caaaatcatg atcggtctgc aaaagccata tgcgggccag gtcctgtatc agggaaccga
     6121 cattacccgg gcgtcggatg aagccctgaa cgaataccgg aaaaacatcg gcgttctctt
     6181 tcagtccggg gcgctgttca gctccatgtc catcaaggaa aacatcgcgc tgcccctgca
     6241 ggagtacacc gacctcgacg cggacaccat cgaccgcgtc attcgaatga aactgggcat
     6301 ggtcaacctg gccggctatg aaaatcacaa cccgtccgag ctttccggcg gcatgaaaaa
     6361 aagggcgggc atcgcccgcg caatggccct cgatccgcgt gtgctttttc tggacgaact
     6421 gtccgccggg ctcgatccga tcacggcggt ggagcttgac gacctgatca tcaaaacaaa
     6481 cgaggctctg ggaaccacca tggtcattgt cacccatgag ctggaatcta tttacaaaat
     6541 agcccatcgg gtgctaatgt tggacaagga ggccagggga atgatcgcgg aaggcagacc
     6601 tctggaatta aaagagaacg cgacggaccc gcgggtcaga agcttctttt tgagacagtt
     6661 gcccgaaaat caggacgaac agaggtttta tgccgactag aagctcaaaa ttttcaatcg
     6721 gcctttttgt cattgtgggc gcgctgatct gcgccggcgt gatcatctgg gtcggcgccg
     6781 ccaaattttt caccaagggc tctttctaca acgtttactt tgacgagtcc gtccagggat
     6841 tgcaggtgga ctcggcgatt aaataccgcg gcgtcgaaat cgggaaagtg cagaagatcg
     6901 acgtggcgcc cgactaccgc ctgatcgaag tgaccatgaa aatcgacctg gaaggcgatc
     6961 tgcagaagca gacgatcgcg acgctgaaga ccgccggcat caccggcatt gtcttcatcg
     7021 agctggaccg gatcaaaccg ggcgaactgg ccgattctcc aaagctcacc ttcaaggcgg
     7081 actacccggt catcccgtcg cgccgttcgg aaatcagccg gctgatcggc gacacgaaca
     7141 tgatcatgca aaacattaaa aaaatagatt tcgagggaat ttccagccag ctcaaaaaca
     7201 ccgcgcaggc gatcgaaaac ttcgtggagg gcaagcgcat cacgagcctg atgactcatc
     7261 tggaaaacac ctcggcaaat ctggacgaga caatcgccgg aatcaacaag accgtcgccg
     7321 aaggcaaggt ggaccgggcc gtcacggaag ccctgggcgt gctgaccgac gcccgcaaac
     7381 tggtcggccg ggccagggag gaaatcggag cgctgaacct gcgcgaaaag tcggctcaaa
     7441 ccgacgccat tcttcagaac atcgacaaaa agaccaagat cattacgacc ggccttgccg
     7501 acacgtcgga aaacctgcgg gtcacctcgg aaaacctcca gaggcttgcc gaaagcctga
     7561 accgggatcc gtcggacctg atttacacca aaccggcgcc gccgcgaaag cccatggaat
     7621 aaagaaagga acgagcaatg gaaaaatccc tacaaaggca ggcccgcttc cacaaaccat
     7681 catccgtcat gccgctcttc gtcatcattg cactcctgtt atgcgcagcc gggtgcgcag
     7741 gcggcggcaa gccgcaatac gacgttcaaa aatacctgct cgcctatccg tcgcccacct
     7801 gggatggctc tgcaaaactt cccgtgtcgg tcaaggtgaa ccggttttcc attgccgccg
     7861 cgtataattc gaccggcatg attttccgag acgacgccta cggtctggac gcattcaact
     7921 actcccgctg ggcggtcaac ccggccgaca tgatcggcga tctccttttg gccgacatga
     7981 gagccggaaa tctttttcag gcggtcctct cgcggtacga cctggaagag gggcggttta
     8041 tcctctcggg cggcatcgaa gaattttatc tgcaaacgga aaagaaaaac agagccgccc
     8101 gcatcgtcat cgccatttcg cttcaggact cccgggaaaa agaaaccggc aaaaaaatga
     8161 tgtatcagaa aacctacacc cgtgaagcgc ccctgcagga agcctcgccc aaaggatact
     8221 gcgccgccgc aagccaggcc atgcaggccc tgtcccgaga gatcatcggc gacgtctatt
     8281 cggcggtcag ggccagaaca ccgtaaagga acaggtgttt catgaaggaa atttccgtcc
     8341 ggagctcatc ccgttttgaa atgatcgaca tcaccgaagc cgttcggaag acggcgcgcg
     8401 aggaaaagat cgaatcgggc atcgtcctag tctatacgcc ccacaccacc gcagcggtca
     8461 cgatcaatga aaacgcggac ccggacgtgc cccgcgatct tctggcggcg ctggaccggg
     8521 ccgttcccct gtccgcgaat taccggcaca cggagggcaa ttccgctgcg cacgtcaaat
     8581 cgtcgatggt cggcgcgtcc gaaatggtca tcatcgaaaa cggccgcctg gtgctgggca
     8641 cctggcaatc catcttcttt tgcgaattcg acggcccgcg atcccgaaaa gttatcgtca
     8701 aatttctcac gtgattagtg aatggtgtat ggtgtatcgc tcttagccac catccgctat
     8761 ccactataaa ccaaagcggg gctgaagccc cgccctacat ttcactcatt gatcgtcact
     8821 tttaactaca agccacggtc ttttacctat cgcccattgc ctattgcccc tcgcctgttt
     8881 ttccaccatt caccactgct gacatgacct ccagcatctg ctcctgaatc agtccgttgc
     8941 tggcgacaag gcccggagaa aaaagatgcc atttttcgcc ggccatatcg gaaaggacgc
     9001 cgcccgcttc ttccaccagc aggcatccgg cggccgtgtc ccagggtttg agcttgagct
     9061 cccagaaacc gtcaaaccgt ccggcggcaa gataggccag gtccagggcc gccgcgcccg
     9121 cgcgccggat cgcctgcgct tttttggcca ttgcgttgaa aaagttcagg ttgttgtccg
     9181 gactttcacg gatgtcatag ggaaagccgg tggccagaag gctgcgggac agatcttcca
     9241 cggaagaaac gcgaagctcc ttcccgttga ggcgcgaccc gcctcccagc gaagcctcaa
     9301 acaggtcgtc ccgaagcgga tcataaacga cgcccaaaac aacgctgccg tctttctcca
     9361 gggcaacgga gacgcagaaa aagggaaagc cgtgggcgta attcgttgtt ccgtccagag
     9421 gatccacaat ccagcgcatc gcggcccgcg cgtttttctc ctttgattct tcggataaaa
     9481 taccatgacc aggataccgg cggccgattt ccgcaaggat gatctcctcc gacattcggt
     9541 ccgcttccgt caccagatcg atggctccct tgtagtaaac cggattgtgc cgggtgaatt
     9601 tttcagcaag cattcttccc gcctttcgcg ccaggtaaac agcaaaatcc gttagttccc
     9661 tcatgaccgg ccattcctcc atatcatcag caatcaacgg tgtttaaacc acaaagccgg
     9721 caaaaaaggt agagcattct taaacattgc gctggtgaat atttaatgct aaacagacca
     9781 aaaatgttgt tggtttgcgg ttcatgacga cagaaaaaaa tgcggattcc cccatccctg
     9841 atttgaaaac ggcgcggcaa aattcaggac agaccctgaa ggaaatgttt gagcgcacac
     9901 ggatcagtgt ggtcaatctg gaagccatcg aaaacggtca gttccatctg ctgcccgtcc
     9961 cgacctacac gcgaaatttc atcaaaatat acgcgcaggc cctgggggtg gacagctccc
    10021 cgctgctcgg gcgctacgaa gaatatctgc aggcgctgaa aaacaaagaa aaggaacagg
    10081 ccgccgtaaa gccgcccccc gcgcccctgg ccgaaatgct gagccgttac aaaatcttcc
    10141 tgtggatcac cttgatcgtc attctcattg cggccgtgtc cattttcgcg agcctttaca
    10201 ataaaccttt gtccgaatcg cagcagggaa tccagcgaac cggggaagcg gaaggccggg
    10261 cagccccggc gcaggtcgca gcgggggctg acgcgctccc cgtcgggatc ggtttgccga
    10321 agccggagga aatcgcggga gtccaaccgc cggagagtca acaggtcatg gcgtcgccgt
    10381 ccattccgtc ctccgccgtt tcacaaactc ccgccgcaca gaaggcgccg gaaaaggaca
    10441 aagccgctca agcggcggca ggcgcggaag aaacgtctct tttgatcatc cgcgccacgg
    10501 agcaaacctg gattcgaatg caggttgacg acaaggaacc ttacgaagtt ttgctggaag
    10561 cgggagaaac gctctcgcaa aaagcgtccc gcttcaacat ggacgtcggc aatgccggcg
    10621 gaatccgaat ccagttggat ggaaaaacca tagagaattt aggcaagtcg ggtcaagtga
    10681 tccacttgcg cttaccctga agccattaaa gaaaagaggg agttttatgt ttggaatagg
    10741 catgccggag ttgctgatca tcctggtcat cattttgatt attttcggcg tcggaaagct
    10801 gccggaaatc ggcagggggc tcggcgaggg aatccggaat ttcaagaagt ccgtctccga
    10861 caagaatgat gcgaacccgc cggcaaaaaa agagggagac aagtaattct gcctccccgt
    10921 tttggctcat gaattcattc gtgaaggcta gaacggaacg tcgtccatcg gcgtgccgct
    10981 gttttgcgcg gagggcgcct cgggcgttgc atcgccggat ttggcctgcc ccttggaatc
    11041 cagcatttgc atattgctgg caataatttc cgttgtgttg cgtttcacgc cttccttgtc
    11101 ttcccaggcc ctggtctgaa tccgtccttc aatgaaaacc agctttccct tgaccaagta
    11161 attgccacag atttccgcca gtttgccgaa cgcaacgatc cggtgccact cggttttctg
    11221 taccctctcc ccgtttttat ctttgtaggt ttcatccgtc gccatggtga agttcgtcac
    11281 catcgtgcca tccggcgtgt aacggacctc cggatctctg cccagtctgc cgatcaatag
    11341 cgccttgttg accatgattg cctccccgtg aaatgagttg gcggcatcat acataacaac
    11401 cccctgggaa gcaaacacat tttttgattt taaagccctt gggccaatgg gttgcggcca
    11461 gaatcgggct tgactaatcc ccaaaaaaaa tatagtcaaa cagactttta aaaagcggag
    11521 aaattatgga agacaatgat cttttaaagg ttcgtctgga aaaaattgcg gcattgaaag
    11581 cagccgggac ggacctgtac cccaacgacg caaaacctca aaatacaacc gaccggatcc
    11641 ttcaggaata cggcggtatg gacagcgatg gtctggcaaa gctcacacag aaattttcgc
    11701 tcgccggacg cctgatggcc atccgcagct tcggcaaggc ggcttttgtc aaaatccagg
    11761 accggaaagg ccaactgcag tgctacattg ccaaaaacat cctcagcgag caggattttt
    11821 ccatctttaa aaagctggac attggcgata ttatttatgt ttccggaaag cttttccgga
    11881 ccaagaccaa cgagctgacg ctggaggccg agtcggtgcg cctgacggcc aaatccattc
    11941 gccccctgcc tgaaaagtgg cacggtctga cggacatcga aacccgctac cgccagcggc
    12001 atctggatct gatctccaac ccggcggtga aggaaatctt catccggcgc agcctgatca
    12061 tcaggctgat ccggcagttc atggaccggc acgattttct ggaagtggaa acccccatga
    12121 tgcagccgaa agccggcggc gccgtcgccc ggccgttcaa aacacatcac aacgcgctgg
    12181 atcaggattt gtatctgcgg atcgcccccg aactttatct gaagcgcctg gtcaccggcg
    12241 gcctggaaag agtgtatgaa atcaaccgca acttccgcaa cgaaggcatc tccacgtttc
    12301 acaacccgga atttacgatg atggagtttt acgcaagcta cgcgacctat gaagatttaa
    12361 tgtccttcac ggaagagttg ttcgccttca tcgcccggga aatcttcgga aaacttgcat
    12421 ttacctacca gggcacggag attgatctga cgccgccgtg gcgtcgtctg gccgtgaaag
    12481 acgcgcttgt ggagatcgcc ggcatgacgc ccgacgaaat ggccgatccg gcgaaagcca
    12541 ttgattttgc gcgcaatgcc gggtgcgaca tcaaggaaac ggacccgccg gggaaaattc
    12601 tgatggcgat cttcgatgaa cgggtcgaaa aaaagctgat ccagccgacc ttcgtcactc
    12661 attatccggt cgccgtttcg ccgctttccc gaaggagcaa caccgacccg gacctggtgg
    12721 accgtttcga gctttacatt tccggacggg agatcgccaa cgccttttcc gaactgaacg
    12781 atccggccga tcagcgggaa aggtttgccg cgcagctcaa agagcgggaa gcgggcgacg
    12841 acgaagccca cgaaatggat gaagactaca tccgaacact ggagtacggc atgcccccga
    12901 cggcaggaga agggatcggc atcgaccgcc tgatcatgct tctcaccgat tccgcgtcca
    12961 ttcgggacgt gattttattt ccgcttctga ggtccaggac ggaatgacga acccgcggtt
    13021 tttcgcgcgg cggcccgaat caacggctta cccacaggac agagcttgat gtcttacgaa
    13081 tttttcatca gcagacggta catgagggcg aaacgcaagc aggtttttgt ttccatcatt
    13141 accttcatat cgattttcgg cattttcctg ggcgtggccg cgctcatcat cgtgctggcc
    13201 gtcatgaacg gctttgaaga agatctgcgc agcaagattc tgggcatccg ctcccacatc
    13261 gaggtgacgg cggacatgag cggcccgatg aaaaattacc cggcggtccg ccgcgaaatc
    13321 gaacaaatcc cgggagtggt cgcttccacg ccgttcattt acacggaagc catgatccaa
    13381 agcccaaacg gcgtcaccgg cgtggtcatc cgcggcatgg agacccaaag cgcgccccgg
    13441 gtcatcaacc tgggaaaaat ccgccagggc agcgtcgatc atctcgacgg gatccccgaa
    13501 gccgtccgtc aaaaactgcc cgcggccgac cgggagctgc cgggcattct gatcggcagg
    13561 gaaatggcca gaaacatggg gatttttctg cacgatccga tcactatcat ctctccggcg
    13621 ggcggaatcg ccacgccgat gggcatggtt ccccgcatga agaaattcgt cgtgaccgga
    13681 atctacgaat cgggattcta cgaatacgat tccaagctgg ctttcctgtc gctggcgagc
    13741 tgccaggatt ttctggaaat gggcgacgcc gtctcgggca ttgacattgt cgtgcgggac
    13801 atctaccagg cggacgtcat cgcccgcaaa atccaaagcc ggctgggctt ccccttttac
    13861 gcccagaact ggatgcagat gaacaaaaac ctgttttcgg ccctcaggct ggaaaagcga
    13921 gtcatgttca ttatcctgag cctgatcgtg ctggtggccg ccttcaacat catcagcgcg
    13981 ctgatcatga tcgtcatgga aaagaacaaa gacatcgcca tcctgaaatc catgggcgcc
    14041 acctccgcaa ccattatgaa aatcttcatc tatcagggat tgattgtcgg aatggtggga
    14101 acttttttcg gctctgtcgc cggccttgcc attgcgctga atctgcagaa aatctctctt
    14161 cttgtggaga aagttttcaa tttcaaaatt ctgccgggcg acgtgtatta cttaagcgaa
    14221 ctgccgtcga aagtgaatta cggcgacgtg gccctgatcg tcgcgggcac gctcgcaatc
    14281 tgttttctgt ctacgattta tccatcctgg agggcgtcgc ggctggaccc ggcggaagcc
    14341 cttcggtacg aataacgcga gggtcttgca aagcatatga tcagactgga aaatttatcc
    14401 aaaaccttcg tgaaggacgg caacaagatt gaagttctga aagggctgaa cctggaaatc
    14461 gccagaggcg actccctggc cgtcgtcggc gtgtccggcg nnnnnnnnnn tttcaactgg
    14521 aatgaaaaga aaatcgcggc gttccgcaac cgaaccatcg gctttgtgtt tcagttcaac
    14581 aaccttctgc cggagttcac cgcactggaa aacgccatga tgccggcgct gatcggtgga
    14641 ctgaacaaaa aagaggccaa tgaccgggcc gcgcgtcttt tgacggaggt cggtctggag
    14701 catcgccttc aacacaaacc gggggagctc tccggaggag aacagcagcg cgtggccatc
    14761 gcccgcgccc tggtgatgga gccggaaatt ctgctggcgg acgaacctac aggaaacctt
    14821 gacacggaaa ccggaaaaaa gatagaagac atcttaattg acttgaatgc cgaaaaaaaa
    14881 ataacgctgg ttgttgtcac gcacaacaga ctgctggcgg aaaggatgtc cggcagaatc
    14941 ggcctgcgcg acgggaagat ttatgatttt taatttcaaa acggtccggc tcttcatcgc
    15001 cgttgtgctt cttttgtttg cgatggaagc ccgagcggac gccccaaaaa aaatatgcat
    15061 tctgcccttt gaggttcacg cgagcaccgg cagcgccgag ctgcaggcgt cggtgcacaa
    15121 cagcctgctc acggaattcc ggcgggaaaa gaaagtggaa gtgatcgccg ccggcgcttt
    15181 cgaaccgggc ggccccgcgc cgggcgaggc ggaagccggt gcggcgggaa aagaactggg
    15241 ggcggactac gtcgtattcg gcagcgttat gcggttcggt gaaaccctga acatcgacgc
    15301 gcaaattatc gatgtcgccg gagaaaaaat cctgtccgcc gtttcggttc agggaaagga
    15361 aacggagggc gccggggcgc tggccggagc cctgaagcgg gaaattctgg gccgggcggg
    15421 gctcacggac aaaatcgcgc gcgtggagat cgcgggcaac cgcaagatcg gcgcggacgc
    15481 cgtcgtgcag aagctcaaaa gcaggcccgg caaggtcctc gacgaagacg acatccgcga
    15541 cgacatcaaa accatcttca aaatggggta ttttctggac gtcagcgccg acgtcacggc
    15601 ggaggccggg ggaaaagtgc tgaccttcaa cgtggtcgaa aaaggcctga tttcggaaat
    15661 ccggctgatc ggcaacaaga agctggaccg cgacgacatc ctggaagcca tgagcgtcaa
    15721 agtgcggcaa agcgtcaatc cggaaaatgt caagcaggac ctgcagaaaa tcaaggcgct
    15781 gtacgacgcc aagggctact acaacgccga aatctccgac cgcctggaaa aagacggcga
    15841 gaaggatttc gttctgatcc tggatatcag ggaaaacgac cgggtctata tccgctccat
    15901 cacgtttgag gggaacgaat cctttaccgc caaggaactg gccggcatta tgaccacaaa
    15961 cgagcgaacc cttctgggct tcataacgga cacgggcatc ctgcagcccc agcagctcaa
    16021 gcaggacgtt cagcgtctga ccgcttttta tttcaacaac ggcttcgtga acgcccaggt
    16081 cggggagccg gaaatccggc acgacgacaa gggcatctat atccatttta ccgtccgtga
    16141 aggaaagcgc ttcaaaatgg gcaaggtgga aatctcgggg gattacctgg aaaaaaccag
    16201 ggatgacctg tacgcttcct taaaaaccaa agccggaagc cactacagcc gggaagcgat
    16261 catgaaggac atcgaaatga tcacgacgac ggccaacgac gaggggtatg ccaacgccga
    16321 cgtctatccc caggtcgcca cccatgaaaa ggagcagctt gtggatttga acttcgtcgt
    16381 gaagaaaggc gaactcatcc acatcgcccg catcggcatt tccggcaaca ccaccacccg
    16441 cgacaaagtg atccgccggt ccctctttat cgcggaggga gacctgtatt cctccagccg
    16501 gctgaaaaaa agctacgaga atctgaacca gcttcgctat tttgaagaag tggacattca
    16561 gacggaaaag gcgccggaca atcagatgga catcaacatc cgggtgaagg aaaaaaacac
    16621 cgggatgttc atgatcggcg cgggttacag cgccgtggac caggcggtga tcatggcgca
    16681 gatcgttcag cagaattttc tgggctacgg gcagaccatg agcctgaagg cctcgctcgg
    16741 ttccacaacc aacaactatg aactgtcgtt taccgagccg tacctgtttg acctgccgct
    16801 gtggttcaag gccgacgtgt ggaaatacaa aaaggaatac gattcctact cgctggacac
    16861 gaaaggcgtg ggggtgaccg tgggccaccc gctgttctgg aaaatcgtgg gctacgtcgg
    16921 ctacaagttc agcatggaca acatccagga catcaatgcc gctactgcgt ccacctacat
    16981 caaggcgcag gcgggcgaac ggacgagcag cgcgctttcc ttcacgatgg tctatgacac
    17041 caccaacgac gtcatgttcc ccagcaaggg catcaaggcc agcgcgtccg tgcagcatgc
    17101 cggcatgccg ctgggcggaa acaccaattt caccaagtac gccaccaccg cgaccggctt
    17161 tgtcccgctc ttctgggaca tggtcttctc cctcaagggc cagatcgggt atctgcagaa
    17221 caatgacgac agcgacgaca agcttccgat ctacgaacgg tatgtgctgg gcggcatcaa
    17281 ctccctgcgc ggcctgcgct acgtcgggcc gaccaatccg ggaaccagcg acgtgatcgg
    17341 cggcaccacc atgctgacca tgaccgcgga aattgttttt ccgctcatca aaaacgcggg
    17401 tatgaagggc gtcgtgtttt acgacacggg caacacgtgg aacggaggct atcacttaaa
    17461 tgacctgaga agcacctgcg gcgcgggcat ccgctggtat tcccccatcg gcccgcttcg
    17521 cctggaatac ggctacgtgc ttgacaacac gggcctgaac gatgacagtg tgggccgctg
    17581 ggaatttacc atcggcatgt tcatgtaaac cggagccagg acgccaaacg tcagggttca
    17641 atcataaacc aatcaaccga gaggagattt tgaaatgaaa aaaaacatgc tgtggatcgc
    17701 aggactgatg ctgcttcttt ttgcctggac aaacacgtcg ctggccgccg accggatagg
    17761 cttcatcaat atgcagacga tcattcagaa ttccaacgca ggcaaaaaag cggccgaaga
    17821 ctttaaaaag ctttttgccc gaaagcagga aagcatcaag accatggaaa acgaagtcaa
    17881 gaaactcaag gacgaactgg ataagcaggg cgccgtgatg accgccggcg tgcgcggcga
    17941 caaggaagcc gcctaccagc gcaagatgcg cgactaccag atcctggtcg acgacaccaa
    18001 caaggagctc cagaagcgcg accaggaata ttcgcagcag ttgatccccg aaatcctgaa
    18061 agtggtccgg accatagccg aaaaggaaaa atacaccctg attctggacg tgagcaccat
    18121 gccgctgccc tatttcgaca aggccggcga catcagcagg aaagtcattg aagacttcaa
    18181 caagacgcag gtcggcaaaa aataattttg tcgcgaaata tgaaaaaaag cattgcggaa
    18241 atagccgccc tggtgggcgg catggtcacg ggcgatgacc ggaagcggat cagcgatatt
    18301 cggccgatcg aggaggcggg cgcggacgat ctttcgtttg tcgcaaatcc aaagtatttt
    18361 aaatggctca aaaccacact cgcgggagcg atcctcgtcc ccccgggaac cgtcgcgccg
    18421 gataaaaacc tgattgccgt agccgatccc tacgcggcgt tcggcaagct gctcacggtc
    18481 ttttatcccg tcgatcacgg ccccgcgggt ataagccctg atgccttcat cgaagaaggg
    18541 gctttcgtct ccccggaagc aaccgtcatg ccccgggcgt tcatcggccg cggcgcccgc
    18601 gttgaaaaag gcgcggtgat ttatcccggc gtgtttatcg gccgcgacgc ttcggtcgga
    18661 gaagattccg tcctgtacgc caacgtc
//
LOCUS       DAOSIM010000015        18441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_3247_length_18441_cov_8.413195, whole genome shotgun sequence.
ACCESSION   DAOSIM010000015 DAOSIM010000000
VERSION     DAOSIM010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 18441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18441
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3247_length_18441_cov_8.413195"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLR47_01495"
     CDS             <1..405
                     /locus_tag="PLR47_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11638.1"
                     /translation="AFAFIRTHVLTKTGFTGNHQRDIALVYAALLHGCCYFSLEYFRK
                     AKGFRFTLEHDQKVFQMGDQVPLAENIRLSVFCPAKALIRVIRNGCEECRATGDRLNL
                     TIDKTGVYRVEAHLKAAGITRPWIFSNPIFVT"
     gene            complement(469..2661)
                     /locus_tag="PLR47_01500"
     CDS             complement(469..2661)
                     /locus_tag="PLR47_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOG11639.1"
                     /translation="MNPAEIIAKAIRESRNALTEAEAKVVLKHYRVPVVEEKIVNNIN
                     ELSVFAREMGYPVVLKGLGSRLTHKTERGLVKLNLKTPEDLQAAAIYIKDAAGDDLEG
                     FLLQPMLEGRREFVAGLFFDAQFGPVIMFGLGGVFTEAIGDVIFRLAPLDEEEANRMI
                     SELRAHKLLGDFRGEKAPNRDALIRVLTGLSEIAMNIPEIREIDINPLLVSPDGRVTA
                     VDALIVLGERGLRNITHAPVEPMAIASLFYPKSIAFIGASADLSKWGQLMFTNVVAGR
                     YAGKVYLVNPRGGEIAGRKVFKTVTEIPDPVDLAVITIPAGKVLPVIDEMRAKGIKNV
                     LLISSGFGETGAEGKALEEELVQKAGKAGILILGPNTMGICNPHCSLYCTGTHVRPKP
                     GDTVLVAQSGNLGTQLLAFAEKEGIGIRAFSGSGNEAMITIEDYLDGFEIDPMTRTVV
                     MYIESVKNGRRFFESARRVGMKKPVIALKGGRTQAGAHAAASHTGAMASNIKVFDAAC
                     KQAGIIQARQSMELLDLSAAFSSLPLPHGNRVAILTLGGGWGVVASDLCAENGLLVPK
                     LSKEIVGKINGMLPPFWSHANPVDLVAEMNTDIHMAIIEELLRWEECDAIIHMGIIGR
                     KIMIQSVLESTVAVDKSYDPKMVADNLELLKIYEQGLVEKTVRIMGKYHKPVIGVYLL
                     TDETTRTVIEIEGQKYKGIVFASPERAVKSLSRMYQYSRWQKVNGSED"
     gene            2763..3269
                     /locus_tag="PLR47_01505"
     CDS             2763..3269
                     /locus_tag="PLR47_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG11640.1"
                     /translation="MSNPSVNQVMVGGRFVGLIGLEEAVRKAVAVCVGKSDSEISDFL
                     VREVSGRNYIPPSILPAYARALLREYKTALNLPVDPEPATGLTILALGAGCARCDQLQ
                     SDIRDVLSEMQIAADVRHVTDLREMSRFGVLGAPALVINNRVVAVGEVPPKSLIRQWI
                     TAALKNMD"
     gene            complement(3677..4453)
                     /gene="mutM"
                     /locus_tag="PLR47_01510"
     CDS             complement(3677..4453)
                     /gene="mutM"
                     /locus_tag="PLR47_01510"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOG11641.1"
                     /translation="MPELPEVETLCRQLQTRIAGKKILSTKVCDHKLDGIGNVRGLRV
                     LGVCRRGKTVEMRLEGGQCIIIHLRMSGRLFWRTGRCRESHTRWRITLEGGGIDLVDP
                     RRFATVRVISDPPEALTNDLMGSFDFRKFLQSQAGRRVPVKTLLMDPKAIAGIGNIYA
                     CEILHASGISPLRPAAALDETEWKKIFRQARRILRRAIAGRGTSISDWRDLDGCPGEN
                     QKALKVYGREGEACVRCGGIIVRIRQSGRSTYCCPRCQKE"
     gene            4638..>5150
                     /locus_tag="PLR47_01515"
     CDS             4638..>5150
                     /locus_tag="PLR47_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11642.1"
                     /translation="MENETLFNEEAEPPGHATGLVVVKKKTADFFSLTTDHQQPSLAK
                     SKPERIYFPFKDSPRSRTFRKKHYPDISRIEWFDWRWQFQNAIRSVQALDAILHLSDN
                     ERQAMMHPSGDLPVAITPYYASLISPDDPSSPLRRCVVPVVDECLHTKGEEEDPLGED
                     ADSPVPGIVHR"
     assembly_gap    5151..5234
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5235..5922
                     /locus_tag="PLR47_01520"
     CDS             <5235..5922
                     /locus_tag="PLR47_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOG11643.1"
                     /translation="KWEKALDYIASNPNVRDVLLSGGDPLTLSDDRLEWLISRLREIP
                     HVELVRIGTKVPVVMPQRITPSLVSMLKKYHPLWMSIHVTHPDELTPEMSAACIRLAD
                     AGIPLGSQTVLLAGINDSVSTMTRLVHGLLQIRVRPYYLYQCDPIPGSSHFRTPISKG
                     LEIIKGLRGHTTGYAVPTFVVDAPGGGGKIPLLPEYAIGWDKGDLMMRNYEGSLFRYP
                     DNHAESKDIH"
     gene            5940..7001
                     /locus_tag="PLR47_01525"
     CDS             5940..7001
                     /locus_tag="PLR47_01525"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11644.1"
                     /translation="MSGNPVCPATARRRTLSIGLTYDLREDYLREGYSLEETAEFDKP
                     DTISAIENVLKKNGYATDRIGHVKALTRRLAAGDRWDLVFNIAEGMHGFGREAQVPAL
                     LDAYRIPYTFSDALGQALTLHKGMTKHIVRDLGIPTPDFAVVSSPEDIGSVRLPYPLF
                     AKPVAEGTGKGITARSRIDNADSLRSACLNLLQTFRQPVLVETYLPGREFTVGIVGTG
                     RQARAIGAMEVLLNPDAEPHAYSYDNKEHYEKLVRYAAVSDSEALQAMEVSLAAWRGL
                     DLKDGGRVDLRSDASGRPHFIEVNSLAGLNPVRSDLPILCGLVGVSYDRLLCDILNSA
                     LDRAGLCGQQLPHPPARKR"
     gene            7009..7995
                     /locus_tag="PLR47_01530"
     CDS             7009..7995
                     /locus_tag="PLR47_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOG11645.1"
                     /translation="MKKILILHSEVPADAGEDELDCLTQAAAVGEAVLALGCRPETLP
                     FGMDLRANIDRIRERQPDVVFNLVETLAGQGSLNYVATALLDVLRMPYTGCRTEAMFI
                     TSNKPLAKKILQQNGIATPPWMTADVDERGSEPHGTYLLKPCWEDASVGLDSGAIVQM
                     QNPQTLRDALRKRQIALGLDCFAEAYIDGREFNIALLASAQEVLVLPPAEILFVDYPQ
                     DKPKVLDYRAKWVENSFEYDHTVRSLTLAPQDGSLAERLRDIALRCWRLFGLRGYARV
                     DFRVDEQGVPWVLEINANPCLSPDAGFAAALDFAGMEYAVAIDAILQDAFKP"
     gene            8014..8499
                     /locus_tag="PLR47_01535"
     CDS             8014..8499
                     /locus_tag="PLR47_01535"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG11646.1"
                     /translation="MKTTAYRQNIRPSDPDAISEIVKGSGFFSAAEEALARELAEEKL
                     EQGEKSSYQFLFAEEDGLVVGYTCCGLIPATAGSYDLYWIAVAQSLRGGGTGSRLLEK
                     TEEMVRNQGGRKIYAETSSRPQYKPTRHFYEKCGYVPEAFLRDFYSPGDSKIIYAKTL
                     I"
     gene            8631..9251
                     /locus_tag="PLR47_01540"
     CDS             8631..9251
                     /locus_tag="PLR47_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOG11647.1"
                     /translation="MRLFPKEENFFEYFEELANKIEEGGQFFLEMTKNRDYTEAKVAR
                     LKELEHEADVITHRTYERMHKTFLTPIDREDIYALVNKMDSIMDVIEATAIRIHMYKV
                     KKPDDEIIKQAEILFQAIKKIKEIVHGLRDMKNSKMILDGCVEINTLENAGDVVLRTI
                     ITNLFIKEQDAIELIKWKEIFERIEEALDVCEDVSNTVEGIVLKHA"
     gene            9244..10248
                     /locus_tag="PLR47_01545"
     CDS             9244..10248
                     /locus_tag="PLR47_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOG11648.1"
                     /translation="MLDSMAFVIFLVAVALVFDFINGFHDSSNSISTIVSTRVLTPKQ
                     AVIWAAFFNFSAAFFIGTAVAHTIGKGIININVIDNLIILSALGGAIAWNLITWYFGL
                     PSSSSHALIGGLIGAGIAKGGIGTLVWSGILKTTAFIVLSPSIGLILGFIFMVLSMNL
                     NRNSNIAKTDKLYRKLQLASSAIYSLGHGMNDAQKTIGIIAIVLYSKGLISSSFNIPY
                     WIIIMCYSVIALGTMFGGWRIVKTMGTKITKLQPIGGFSAEAAAACAIIGSSIAGIPV
                     STTHTIAGAIVGVGATKRLSAVRWGVAGNIIWAWVLTIPIAAAISATLYLSLQYFIFQ
                     "
     gene            10415..11680
                     /locus_tag="PLR47_01550"
     CDS             10415..11680
                     /locus_tag="PLR47_01550"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOG11649.1"
                     /translation="MPEKQLPFTHKQLSAILEKYPTPFHLYDEKAIRNNARAFAKAFS
                     WNRGFKEFFAVKACPNPYIMKILHAEGFGADCSSMAELLLAQKTGITGEDIMFSSNDT
                     PAAEFVQARKLKATINLDDITHIDFLEKHAGLPDLICCRYNPGPLKKGNVIIGHPEEA
                     KYGFTREQLFEGYRICRDKGIRRFGIHTMVASNELDPDYFVETAEILFHLSVELSHAL
                     NIRFEFANLSGGFGIPYRPEQKAIDLKAMTRGIREKYEKIIVTGGLAPLKIFTESGRF
                     ITGPHGYLVSKVLHIKDTYKKFAGLDACMANLMRPALYGAYHHITVVGKERRPHDAMY
                     DVTGSLCENNDKFAIDRMLPRLAPGDIVVIHDAGAHGHSMGFNYNGKLRSAELLLRED
                     GSVVQIRRAETVEDYFATLDFRGLKKFEG"
     gene            11808..12290
                     /locus_tag="PLR47_01555"
     CDS             11808..12290
                     /locus_tag="PLR47_01555"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HOG11650.1"
                     /translation="MAKEKIPSTPAVLALKKSAAIFTLHTYAYEEHGGTRVSSQKLGV
                     DEHSVIKTLVMEDENANPFIILMHGDREVSTKALARAMGVKSVKPCTPETAHRHTGYF
                     VGGTSPFGIKKRLNLYMEKTISDLPEILINAGSRGLLARMPSAELVRILSPVEVSVAI
                     "
     gene            12346..12903
                     /gene="trxC"
                     /locus_tag="PLR47_01560"
     CDS             12346..12903
                     /gene="trxC"
                     /locus_tag="PLR47_01560"
                     /inference="COORDINATES: protein motif:HMM:NF008229.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HOG11651.1"
                     /translation="MPDNVIIRCLNCGTKNRIPQQKFQRRPTCGHCHAPLDELIIRCL
                     KCGTKNRLPEERLGSKPLCGKCGEALVIARQNIQPVEVTDDSFAREVLSAETSVLVDC
                     WAPWCGPCKSLAPTIDELATDYANGVKVAKLNVDENPATATQYGIRSIPTLLLFRDGR
                     LVERLVGALPKQEIEQHLLAIIKTN"
     gene            12924..13445
                     /locus_tag="PLR47_01565"
     CDS             12924..13445
                     /locus_tag="PLR47_01565"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOG11652.1"
                     /translation="MEKNRTLTYACKIFSVWEEDLLLQNGKKTRQSWIEHKPTVAVVP
                     VNEKKELLLIRQFRIPAGKNLLEIPAGSMDHGEESPDACASRELAEETGWRAKKLTPL
                     FAGYLLPGYCNEFMYFFLAQDLVFEPLQPDEDECIEVIPTSFERAATLLKSGELIDAK
                     TALGIMLAENYLK"
     gene            complement(13448..13984)
                     /locus_tag="PLR47_01570"
     CDS             complement(13448..13984)
                     /locus_tag="PLR47_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11653.1"
                     /translation="MLKYLKDLIFLIILSLILTGCATKAVRDGAQEPMGQVKKANGLF
                     SSDPSDRELFDEALTFLSGSGQEADYSEAKIRLGNLVAQFPESKWAPGAKALLSTMER
                     IAALQAELKDQKIEAQQEQAKLKKEIEGLKNSVRQAEEKNSAELIRLQQENEQLKNDI
                     RQLKNLEIRLEQREKQLR"
     gene            complement(13990..15363)
                     /locus_tag="PLR47_01575"
     CDS             complement(13990..15363)
                     /locus_tag="PLR47_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOG11654.1"
                     /translation="MSLTISRKLLLGYLFMAFLTVLASAFALFNLQKLSGGAYKITHH
                     HFVLMENSKQLMDILLAQESAEKKYLILKDPELEQIFWKRSVEFQEKLAETGRLKLDR
                     RSKGALSEMAVLHRRLKDLFSEEALLVQTGKLQEAQAISDTTGRAVIDAMAMQLRDIQ
                     KNVNQDINNEMNRIASQGRGAVHMTLGLSLLSLVLGITLALVITRNISKPLKQLQAAT
                     RLIAEGNLDHRIEARRNDEIGALANSFAHMTKRLKVLEALNLDASPLTGLPGNLAIEN
                     RIEQLLAKGKSFSLCQVDLDNFKPFADQYGYAWGSEVIKEVAGLLQGYIDEMKLKDMF
                     LGHIGGDDYVLIGDPGQVESICKKLVVDFAGRILSYYRPEDAQKGFFIGRNRQGNVQK
                     FPLITITAAIVTDDGSRFKNPLEMARLVAELKEYAKKMPGSNYVTEEEVERQKAVAPQ
                     NSQSTLE"
     gene            15652..16950
                     /locus_tag="PLR47_01580"
     CDS             15652..16950
                     /locus_tag="PLR47_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOG11655.1"
                     /translation="MIYNEEFETLPREALKALQVKRLQQVLQRVYHTVGYYKKSMDAA
                     KIAPEHIRSLDDLKKLPFITRADLRNNYPFGLFAVPMSNIVRLHASSGTSGRSSVFSY
                     TRRDIETWTDLIARSLVAAGLNKNDIVHNALSYGLLPGGLGLHYGIEKIGASVIPMSD
                     GNTKRQILLLQDFGPTVLCATPSYALHLAEEGKAMGVDMKSLQLRVGIFGAEIWSDAT
                     RDAIENAFNIKALNIFGLSEMMGPGIAMECLEGRRGMHIFEDHFIVETIHPETGDVLP
                     EGEEGELVFTSLTREAFPLVRYRSGDISRLIPEPCRCGRTHIRMDRVLKRSDDMLTIR
                     GINIFPVQVEAILKEIEGVEPDYQLIIDKVGALDAVELRVEVGEKFFSESGGVKELQK
                     IEKRIVKDMKDYLSISPRVKLVAPQTLKESGKKVIDKRTI"
     gene            16973..17404
                     /locus_tag="PLR47_01585"
     CDS             16973..17404
                     /locus_tag="PLR47_01585"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOG11656.1"
                     /translation="MKVEQISVFLENKPGTLEHATRILKENNINIRTLSLAETADFGI
                     LRLIVNDVEKTNKVLKEAGFRVSKTVVVAVEVPDQPGGLHSIMDVLNKEAINVEYLYA
                     FVEKSGQNAVIIFRFDAPEKAIEVLLKHKFTVVPGAKLYEF"
     gene            17558..18085
                     /locus_tag="PLR47_01590"
     CDS             17558..18085
                     /locus_tag="PLR47_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11657.1"
                     /translation="MVGDYIERKFRNVNLWVPSRDTGIDLLVTDSNNKNSLSLQVKFS
                     RDFLTTHMSAIFQKPLRACGWWSLNREKIEKSKADYWVFVLIGFEHRTTDFVIIKPSD
                     LLTRLLAIHNKSKTIQSYLWVTEKNRCWESRSLKRQEQLAIAQGQYSNIERDFSRYLN
                     NWSPIQALNGKKITL"
     gene            18162..>18441
                     /locus_tag="PLR47_01595"
     CDS             18162..>18441
                     /locus_tag="PLR47_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11658.1"
                     /translation="MSLFFQHILELYSNAEYATAREELRNFEKANKPFPDCIYKEIDY
                     AESNKYRKIRDENVFPIRFLWLFELKEQIVSGVVMPLERSYYNEVLLAN"
BASE COUNT         4257 a         5334 c         4515 g         4251 t
ORIGIN      
        1 gccttcgcgt tcatccgaac gcatgtttta accaaaaccg gtttcaccgg aaaccatcaa
       61 cgggacatcg ccctggtgta tgcggcgctc cttcatggat gctgctattt ttccctggaa
      121 tatttccgca aggcaaaagg ttttcgattc accctggagc atgaccagaa ggttttccag
      181 atgggagacc aggttcctct ggccgaaaac attcgattat ccgtcttctg ccccgccaaa
      241 gccctgatcc gcgtcatcag aaacggctgt gaagaatgcc gggcaaccgg cgatcgatta
      301 aatttaacaa tcgacaaaac aggggtttac cgcgtcgaag cacacctgaa ggcggccgga
      361 ataacccggc catggatttt ttccaacccg atttttgtaa cttgaggcgt tgggggcgtc
      421 ttcattcgga ttgttccggg tgaaaacatg aaaatacata ttgagttgtt agtcttcaga
      481 tccattcacc ttttgccagc gggaatactg gtacattctg gacagggact tcaccgctct
      541 ttccggactg gcaaaaacaa tgcccttgta tttctgacct tcgatttcga tgaccgttcg
      601 ggttgtttca tcagtgagca gatagacgcc gatcaccggc ttatgatatt tccccataat
      661 ccggacggtt ttctccacta gcccctgttc gtaaattttg agcagctcca gattatcggc
      721 caccattttc gggtcatagg acttatccac cgcgaccgtc gattccaaaa ccgattgaat
      781 cattattttc ctgccgataa tgcccatgtg aataatggcg tcgcattctt cccaccgcag
      841 cagctcttca atgatcgcca tgtgaatgtc ggtgttcatt tccgccacca gatccacggg
      901 attggcatgg ctccagaacg gcggcagcat accgttgatt tttcctacaa tttctttgga
      961 cagcttcgga acgagcaggc cgttttccgc acacaaatcc gaggcgacaa cgccccatcc
     1021 tccccccagc gtcaaaatgg caacccggtt tccgtgaggc aacggaagag atgaaaaagc
     1081 cgctgataaa tcaagcagtt ccatcgactg cctggcctga atgatgcccg cctgtttgca
     1141 ggctgcgtca aaaaccttga tgttggatgc catggccccg gtatggcttg ccgccgcgtg
     1201 cgcccccgcc tgcgtgcgtc cgccctttag tgcaatgacc ggtttcttca ttcctacccg
     1261 tctggccgat tcaaaaaatc ttcggccgtt tttgacgctt tcgatataca tgaccaccgt
     1321 cctggtcatg gggtcgattt cgaagccatc cagatagtcc tcaattgtga tcatcgcctc
     1381 attgccggag ccgctgaacg cgcggatgcc gatgccttcc ttttcggcaa acgcgagaag
     1441 ctgcgttccc agattgccgg attgcgccac cagaaccgtg tctcccggct ttggccgaac
     1501 gtgagttccc gtgcagtaaa gagagcagtg cggattacat attcccatcg tattggggcc
     1561 aagaatcaaa attcccgcct tgcccgcttt ttgaaccagt tcctcctcaa gggctttgcc
     1621 ttcagccccc gtttccccga atcctgacga gatgagcaat acgtttttaa tccccttggc
     1681 ccgcatttca tcaatcaccg gcagaacttt tcccgctgga atggtgatga cggccaggtc
     1741 gaccgggtcg ggaatttccg taacggtttt aaatactttt cttccggcaa tctccccgcc
     1801 tctgggattg accaaatata cctttccggc ataacggccc gcgaccacat tggtaaacat
     1861 caactgtccc cacttgctga gatccgcgga agctccgata aatgcaatgg attttgggta
     1921 aaacaggctt gcgatggcca tcggttccac gggtgcatgc gtgatatttc gcaggccccg
     1981 ctccccaagt acaatcaatg catccactgc tgttacccgg ccgtcgggcg ataccagaag
     2041 gggattgatg tcgatttccc tgatttcggg gatattcatg gcgatttcag acagacccgt
     2101 caatacccga atgagcgcgt ccctattcgg agccttttct ccgcggaaat cccccaggag
     2161 cttgtgggcc cgcaactccg aaatcatccg gttggcctct tcttcgtcca ggggagccag
     2221 ccggaagatc acatctccga tggcctccgt aaaaacccct cccaaaccga acatgatgac
     2281 cggaccgaac tgcgcgtcaa aaaacaggcc ggccacaaat tcccttcttc cctccagcat
     2341 gggctggagc agaaatcctt ccaaatcatc tccggccgca tcctttatat atatggcggc
     2401 cgcctgaaga tcttccggag tcttgagatt gagcttgacc aggcctcttt cggtcttatg
     2461 agtaagcctg ctgcccaagc cctttaaaac gaccggatag cccatttccc gggcaaaaac
     2521 acttaattca ttgatattat tgactatttt ttcttcaacc acgggaacgc ggtagtgctt
     2581 caagacaacc tttgcctcgg cttcggtcaa tgcgtttctg ctttccctga tcgctttcgc
     2641 tatgatttct gccggattca tgaatctccc tgtcaatatt tgcttgacgt atccttattg
     2701 tattataatc ttgggccgaa tctgccgctg tgattttgcg gctcccgttg atgaggaatc
     2761 atatgtccaa tccatccgta aatcaagtaa tggttggcgg ccgcttcgtc ggcctgatcg
     2821 gtttggaaga agccgtccgg aaagccgtcg ccgtctgcgt tggtaaaagc gattcggaaa
     2881 tctccgactt tctcgtccgg gaggtctccg gccggaatta cattcctccg tcaattctcc
     2941 cggcctatgc ccgcgccctg cttcgcgaat acaaaacggc gctgaacctt cccgtcgatc
     3001 ccgagccggc aaccggtttg accattcttg ccctgggagc gggctgcgcc cgctgcgatc
     3061 aattgcaatc ggacatccgt gatgttttat cggagatgca gattgccgcc gatgtccggc
     3121 atgtgaccga ccttcgggaa atgtccaggt tcggcgttct gggcgcccct gcgctggtca
     3181 tcaacaacag ggtcgtggcc gtgggcgaag tcccgcccaa aagcctcatc cgccaatgga
     3241 tcaccgccgc tttgaaaaac atggattaat gcctcgtccg cgcatttggc ttcggaaaaa
     3301 ctattctccc aatctttttt cgagaacatc ccgccttacg ttttcctcgt tacactcacc
     3361 gcattgctcg ttttcccatt accatctctt tttgtcagtc tgatcctgtc gaaggacgct
     3421 gcccatccac tattcatcag tcaccatcca ccgtggcggt cacgaagacc cgccctacga
     3481 ttccctcgtc actcgccgct ttccactcac cgctcattgc tctgcgtttt ttactacaag
     3541 ccatgagcta tgagctttcc cctctttagc cttccgctag cgcaaccctt tgtcaccctg
     3601 agcctgtcga agggtgacgc ctatccacta ttcaccagtc accatccact gtctcctatc
     3661 gcccatggcc tatcgcctat tccttctgac aacgcgggca gcaatacgta ctgcgcccac
     3721 tctgccggat ccggacaatg atcccgccgc atcgcacaca ggcctcccct tcccgtccat
     3781 ataccttcag cgccttctgg ttttcacccg gacagccgtc aagatccctc cagtcggaaa
     3841 tggacgttcc ccgcccggca atggctctcc tcagaattcg ccgggcctgt cgaaaaatct
     3901 ttttccactc cgtttcgtcc agcgcggccg ccggccgcag cggggaaatg ccggaagcgt
     3961 gcaggatctc gcaggcgtaa atgttgccga tgccggcaat cgccttcggg tccatgagca
     4021 gtgttttgac gggaaccctg cgcccggcct ggctttgcag gaattttcgg aaatcaaagc
     4081 ttcccatcag atcattggtc aaagcctccg gcggatcaga gattacccga acggtcgcga
     4141 agcgacgggg atcgaccaga tcgatgcctc ctccttccag cgtgatccgc caccgcgtat
     4201 gggactcgcg gcagcggccc gtcctccaaa acaaacggcc cgacatccgc agatggatga
     4261 tgatgcattg gcctccctcc aggcgcatct cgacggtctt gccccggcgg caaaccccga
     4321 gaacccgcag cccgcggaca tttccgattc cgtccagttt gtggtcgcag actttcgttg
     4381 acagaatttt ctttccggca atcctcgtct gcaactggcg gcacagcgtt tcaacttccg
     4441 gcagttccgg cattcactcg tcctcttcgc ggcgtaaaaa aattcgccgc tcacttttta
     4501 aaaatttttt tcattttttc tttcaaaatg caaaataaat gttgacaaat ttttttgctt
     4561 gcgtaaattc aaaaatcaat aaagtttatt tttccggata attttttagc ggaagaacgc
     4621 ttccggaagg aggatgaatg gagaacgaga ccctgtttaa tgaggaggcg gaacctcctg
     4681 gtcatgcgac cggtcttgtt gtggtaaaga aaaaaactgc cgattttttt tcgttgacca
     4741 cggatcatca gcaaccctcc ctggcaaaat caaagccgga acggatttat tttcccttta
     4801 aagattctcc ccgctcacgc actttccgca agaaacacta cccggacatc agccgcatcg
     4861 aatggtttga ctggcggtgg cagtttcaaa acgccattcg cagcgtccag gcgctggacg
     4921 ccatccttca tctatccgac aacgaacgcc aggccatgat gcacccctcg ggcgacctcc
     4981 ccgttgccat cacgccttac tacgccagcc tgatttcccc ggacgacccg tcttctccgc
     5041 ttcgccgctg cgtcgtcccg gtggtggacg aatgcctgca caccaagggc gaagaggaag
     5101 accctctcgg cgaggacgca gattctccgg tccccgggat tgtgcatcgc nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnncaagtg ggaaaaagcg ctggactaca tcgcctccaa tccgaatgtc
     5281 cgcgacgtgc tcctttccgg cggtgatccc ctgacccttt ccgacgaccg gctggaatgg
     5341 ctgatttcga ggcttcggga gattccccat gtggagctgg tccgcatcgg cacgaaggtc
     5401 cccgtggtga tgccccagcg catcacaccg tctcttgttt ccatgctgaa gaaataccat
     5461 cccctctgga tgagcattca tgtcacgcac cccgacgagc tgacccccga gatgagcgcc
     5521 gcctgcatcc ggctggccga cgccggcatt ccactgggca gccagacggt cctgctggcc
     5581 ggcatcaacg actccgtctc caccatgacc cgcctggttc acggacttct gcagatccgc
     5641 gtccgtccct actatctgta ccagtgcgat ccgattccgg gctcgtccca tttccgcacg
     5701 cccatcagca aaggactgga aatcatcaag ggcttgcgcg gacacaccac cggctacgcc
     5761 gtgccgacct tcgtcgtgga cgcccccggc ggcggcggaa aaattccgct ccttcccgag
     5821 tatgccatcg ggtgggacaa aggcgacctg atgatgcgca attacgaagg cagcctcttc
     5881 cgctacccgg acaatcacgc cgaatcaaag gatatccact aggatattga agatagctta
     5941 tgtccggtaa tccagtctgt ccagcgaccg cccgccgccg aacgttatcg atcggcctga
     6001 cctacgacct gcgcgaagat taccttcgcg aaggatacag cctcgaagaa accgccgagt
     6061 tcgacaagcc cgacactatt tccgccatag aaaacgtcct caaaaaaaac ggctacgcca
     6121 ccgaccgcat cggacacgtc aaggcgctca cccgccgtct ggccgcgggc gaccgctggg
     6181 accttgtctt caacatcgcc gaaggaatgc acggtttcgg ccgggaggcg caggttcccg
     6241 cgctcctgga cgcctaccgg atcccctata ccttttcgga cgccctcggc caggcgctga
     6301 cgctccataa gggcatgacc aagcatatcg taagagacct tggcattccc acgccggact
     6361 tcgccgtggt ttcctcaccg gaagatatcg ggagcgtccg gctgccctat cccctgtttg
     6421 ccaagcccgt ggcggaagga acgggcaaag gcattaccgc ccgctccaga atcgacaacg
     6481 cggacagcct gcgctcggcg tgcttgaacc ttctgcagac tttccgccag ccggtgctgg
     6541 tggaaaccta tctgcccggg cgggaattta ccgtcggcat cgtcggaacg ggcaggcagg
     6601 cccgcgccat cggcgccatg gaagtgctcc tcaatccgga tgccgaaccg catgcctatt
     6661 cctacgacaa caaggagcac tatgaaaaac tggtgcgcta tgccgccgtt tccgattcgg
     6721 aagccctgca ggccatggag gtctccctgg cggcatggcg aggattggat ctgaaggacg
     6781 gaggacgggt ggacctgcgc tcggacgctt cgggcaggcc tcattttata gaagtgaatt
     6841 ccctggccgg cctgaatccg gttcgctccg acctgccgat tttgtgcgga ctggtgggcg
     6901 tatcctatga ccggctcctc tgcgatatcc tgaactcagc cctggaccgg gcggggcttt
     6961 gcggccagca gctcccccat ccgcctgcgc ggaaacggta aacgccatat gaaaaaaatc
     7021 ctgattctgc acagcgaagt tccggcggac gcaggcgaag acgaactcga ttgcctgacg
     7081 caggcggcgg ccgtcggcga agcagtcctc gcgctgggct gccggccgga aacgctcccg
     7141 ttcggaatgg atctgcgcgc caacatcgac cgcattcgcg agcggcagcc ggacgtggtc
     7201 ttcaacctgg tggagacgct tgccgggcaa ggcagtctga attatgtcgc gaccgcgctt
     7261 ctggacgttt tgcgcatgcc ctacacggga tgccggaccg aagccatgtt catcacctcc
     7321 aacaagccgc tcgccaaaaa aatcctgcag caaaacggca ttgccacacc gccctggatg
     7381 acggcggacg tggacgaacg aggatcagag ccacatggca catatcttct caagccctgc
     7441 tgggaagacg cctcggtcgg tctcgacagc ggggccatcg tgcagatgca aaatcctcaa
     7501 acccttcggg acgcgctgcg gaagcgccag atcgcgctcg gcctggactg ctttgccgaa
     7561 gcctacattg acgggcgtga gttcaatatt gcgcttttgg cttccgctca ggaagttctg
     7621 gtgctgccgc ccgcggaaat tctttttgtg gactatccgc aggataaacc aaaagtgctt
     7681 gattaccgcg ccaagtgggt tgagaattcc tttgaatacg accacaccgt ccgctcgctc
     7741 accctcgcgc cgcaggatgg aagcctcgct gaacgactgc gggacatcgc ccttcggtgc
     7801 tggcgcctgt tcgggcttcg cgggtatgcg cgcgtggatt tccgcgtgga tgaacagggc
     7861 gttccctggg tgctggaaat caacgccaac ccctgcctct cgccggatgc aggctttgcc
     7921 gccgcgctgg attttgcggg catggaatac gccgtcgcca tcgacgccat ccttcaagac
     7981 gcctttaaac cgtaaacagg ggggaacgat tttttgaaaa cgaccgccta ccggcagaac
     8041 attcggccgt ccgacccgga cgcgatttca gaaattgtga agggaagcgg ctttttttcc
     8101 gcagcggaag aggcgctggc ccgtgaactg gccgaggaga agctggagca gggtgaaaag
     8161 agctcctatc agtttttgtt tgccgaagaa gacggactgg tcgtcgggta cacctgctgc
     8221 ggactgatcc cggccaccgc cggcagttac gacctttact ggatcgccgt ggcgcaatcc
     8281 ctgaggggcg gcggaaccgg ctcacgtctt ttggagaaaa ccgaagagat ggtccggaat
     8341 cagggaggaa ggaaaattta cgcggaaaca tcttcccgcc cgcaatacaa gccaacccgt
     8401 catttttatg aaaaatgcgg ctatgttccg gaagcgtttc tcagggattt ctattccccc
     8461 ggcgacagta aaattattta cgctaaaacc cttatataac aggtcttaac gcctttcccg
     8521 ccccgcgtcc gtacgataaa atcttgcaat ttccacgata attcgctaga gcacttctgg
     8581 cttttggatc accccaaccg cccatcgcag gaaaaaaggg gaatttgttt atgcgcctgt
     8641 ttccgaagga agaaaatttc tttgaatatt ttgaagaact ggccaataaa attgaagaag
     8701 gcggtcagtt tttcctcgaa atgaccaaaa accgggacta taccgaagcc aaagtcgccc
     8761 ggcttaagga actggagcat gaagcggatg tcattacgca cagaacatac gaaagaatgc
     8821 acaaaacctt cctgacgccc atcgaccgtg aagatattta cgcgctggta aacaagatgg
     8881 acagcattat ggacgtcatc gaggccaccg ccatccgcat ccacatgtat aaagtaaaga
     8941 aaccggatga cgaaatcatc aaacaggcgg aaattctctt tcaggccatc aaaaaaatca
     9001 aagaaatcgt tcatggtctg cgagacatga aaaactccaa aatgattctg gacggctgtg
     9061 tggagatcaa cacccttgaa aatgccggcg acgtcgtttt aaggaccatc atcaccaatc
     9121 tcttcatcaa agaacaggac gccattgagc tgattaaatg gaaggaaatc tttgagcgca
     9181 ttgaagaagc gcttgatgtt tgtgaagacg tctccaacac cgtggaaggg atcgtcctga
     9241 aacatgcttg attcaatggc atttgttatt tttcttgttg ctgttgccct tgttttcgat
     9301 ttcattaatg gttttcatga ttcctccaat tccatttcaa ccattgtttc caccagagtc
     9361 cttaccccca aacaggccgt catatgggcc gcatttttta attttagcgc cgcatttttc
     9421 ataggcaccg cagtcgctca cacgattggc aagggcatta tcaatatcaa tgttattgac
     9481 aatctaatta ttctttccgc gctgggcggc gccattgcct ggaatctcat tacatggtat
     9541 tttggtttgc cgagcagttc ttctcacgct ctgattggcg gtctcatcgg cgcaggcatc
     9601 gccaaaggcg gcatcggcac actggtgtgg tccggtatcc tcaaaacaac cgctttcatt
     9661 gtcttatcac cttccattgg tttgattctt ggttttatct tcatggtttt gtccatgaat
     9721 cttaaccgga attccaacat tgccaaaaca gacaagctct accgaaaatt gcagctggcc
     9781 tcatcggcaa tttattcgct gggtcacggc atgaatgatg cgcaaaaaac aattggtatc
     9841 attgccattg ttctttatag caaaggtttg atcagttcga gttttaacat tccctactgg
     9901 atcattatta tgtgctactc cgtcatcgcg ctggggacta tgttcggcgg ttggcggatt
     9961 gtgaaaacca tgggaaccaa aattacgaaa cttcagccga ttggcggttt cagcgcagaa
    10021 gccgcggctg cctgcgcgat tatcggttct tccattgcgg gtattccggt cagcacaaca
    10081 cacacgatcg cgggagccat tgtcggcgtc ggcgccacga aaagactttc tgccgttcgt
    10141 tggggtgtgg ctggaaatat catctgggcc tgggtattga ccatccccat tgccgctgcc
    10201 atctccgcca ccttgtatct ttctctgcaa tatttcattt ttcaataatc aaccacctcg
    10261 cggcaagctg cgcagaatga acgcccgttc cgcaacaagg gattccccta tcctgggtta
    10321 tccgccccca gggaaaatag tattgataat ttttgttttt tcccgtaaat cttccacaat
    10381 aatttaaaaa tgttttattt tgggagaatc gccgatgccg gaaaaacaac tgccttttac
    10441 ccataaacaa ctgtccgcca ttcttgaaaa atatccgacg ccctttcatc tttacgacga
    10501 aaaggccatt cggaacaacg cccgcgcctt tgccaaagcc ttttcctgga accggggctt
    10561 caaggaattt tttgccgtca aggcctgtcc caatccctac ataatgaaaa tccttcacgc
    10621 cgagggattc ggcgcggact gcagctccat ggcggaactg ctgctggcgc aaaaaaccgg
    10681 catcaccggt gaagacatca tgttttcttc caacgacacg ccggccgccg aatttgtcca
    10741 ggcccggaaa ctcaaggcaa ccatcaacct ggacgacatc acacacattg attttctgga
    10801 aaaacacgcc ggacttccgg atctgatctg ctgccgctac aatccgggcc ccctgaagaa
    10861 agggaacgtc atcatcggcc atccggaaga ggccaaatac ggattcacgc gcgaacagct
    10921 ttttgaaggc tatcgcatct gcagggacaa aggcatccgt cgcttcggca ttcataccat
    10981 ggtcgcctcc aatgagctgg acccggatta ctttgtcgaa acggcggaaa ttctttttca
    11041 tttgagcgtt gaattatccc acgccctgaa catccgcttt gaattcgcca acttgagcgg
    11101 aggattcggc attccttacc ggcccgaaca aaaagccatc gatctgaagg ccatgacccg
    11161 cggcattcgc gaaaagtatg aaaagatcat cgtgaccggc gggttagccc ccctgaagat
    11221 ttttacggaa agcggccgct tcatcaccgg ccctcacggc tacctggtat caaaggtcct
    11281 gcacatcaag gacacgtaca aaaaattcgc cggcctcgat gcctgcatgg ccaatctgat
    11341 gcggcccgcg ctttacggcg cctaccatca catcaccgtc gtcggcaagg aaaggcggcc
    11401 ccacgacgcc atgtatgacg tcaccggctc cctttgcgaa aacaacgaca aattcgccat
    11461 cgaccgcatg ctcccccgcc tggcccccgg agacatcgtc gtgattcatg acgcgggcgc
    11521 gcacggccat tccatgggct tcaattacaa cggcaagctc cgctccgccg aactgcttct
    11581 tcgggaagac ggctccgtcg tccagatccg gcgcgccgaa acggtggagg attatttcgc
    11641 gacgctggac ttcagggggt tgaaaaagtt cgaaggataa aaacggcaag gggttcgggg
    11701 tcagaggtca ggggctaaat acgcactacc cccttcttgc ctcttaccca ttacctctga
    11761 cctgtctttc ccttgccttt tcccatccac gtcattatat tgaccgcatg gcaaaggaaa
    11821 aaatcccatc aacgcccgcg gtcctggccc tgaaaaaatc ggcggcaatc tttacactgc
    11881 atacctatgc ctacgaagag cacggcggca ccagagtctc ttcgcaaaag ctgggcgtag
    11941 atgagcatag cgtgattaaa acgctggtga tggaagacga aaatgcgaac cccttcatta
    12001 tcctgatgca cggggaccgg gaggtttcca ccaaggccct ggcccgcgcg atgggcgtaa
    12061 aatccgtcaa accctgcacg ccggagaccg cgcatcggca caccggttat tttgtcggcg
    12121 gaacgtcccc gttcggcatc aaaaagcgtt tgaatcttta catggaaaaa acgatttccg
    12181 atcttccgga aattctcatc aatgccggct cgcgcggact gctggccaga atgccgtctg
    12241 cggaactggt ccgcatcctg agccctgtcg aggtcagcgt tgcgatataa tttgccattt
    12301 gagcggaaaa aagagataat gcctcaaaat ttcgggagat gatgtatgcc ggacaacgtg
    12361 atcatccgct gcctgaactg cggaacaaaa aaccgcatac cgcagcaaaa atttcagcgc
    12421 cgacccacct gcggtcattg ccacgcgccg ctggatgaac tgattatccg ctgcctgaaa
    12481 tgcggcacca aaaatcgtct gcccgaagag cgcctcggct caaaacccct ttgcggcaaa
    12541 tgcggcgaag cgctggtgat tgccaggcaa aacatccaac ccgtcgaggt caccgatgac
    12601 tcctttgccc gcgaggttct gtccgcggaa acctccgtgc tggtggactg ctgggcgccc
    12661 tggtgcggcc cctgcaaaag ccttgcgccg accattgacg agctggccac ggattacgcc
    12721 aacggcgtca aagtcgccaa attgaacgtg gacgaaaatc cggcaacggc cacccaatac
    12781 ggcatccgca gcattcccac gctgctgctt ttccgggacg gccggctggt cgagcgcctt
    12841 gtcggcgcac tgccgaaaca ggaaatcgaa cagcatcttc tcgccattat taaaaccaac
    12901 tgaaacggaa accgataccc ttcatggaaa aaaacaggac cctgacgtat gcatgtaaaa
    12961 tcttctctgt ctgggaagaa gacctgctgc tgcaaaacgg gaaaaaaacc aggcaaagct
    13021 ggattgaaca caaacccacc gtggccgttg tccccgtcaa cgagaaaaaa gaactgctgc
    13081 tgattcggca gttccgcatc cccgccggga aaaacctgct ggaaattcca gccggttcca
    13141 tggaccacgg ggaagaatca ccggacgcct gcgcgagccg ggaactggcc gaagaaacag
    13201 gctggcgggc taaaaagctt acgccccttt tcgcgggcta cctgcttccg ggatactgca
    13261 atgaattcat gtattttttt ctggcgcagg acctggtttt tgaaccgctg cagccggatg
    13321 aagacgaatg catcgaagtc attcccacaa gcttcgaaag ggccgccacc cttttgaaaa
    13381 gcggtgaatt gatcgacgcg aaaacggcat tgggaatcat gctggctgaa aactatttaa
    13441 aataaggtca gcgcagctgc ttttctctct gctccagccg aatttccaga tttttcaact
    13501 gccggatgtc gtttttcagc tgctcgtttt cctgctgcag ccggatcagc tcggccgaat
    13561 tcttttcctc tgcctgacga acgctgtttt tcaaaccttc gatttctttt ttgagcttcg
    13621 cctgctcctg ttgggcttcg atcttctgat ccttcaattc agcctgcaac gcggcaatcc
    13681 tctccatggt tgaaagcagg gccttcgcgc ccggcgccca tttgctctcc ggaaattgcg
    13741 caaccagatt ccccagccgg atttttgcct cgctgtaatc cgcctcttgc ccgcttccgg
    13801 acaaaaaagt cagcgcttca tcgaacagtt cccggtccga aggatcgctg ctgaacaggc
    13861 cgtttgcttt tttcacctgg cccatgggtt cctgagcgcc gtcccggacg gcctttgtcg
    13921 cgcagccggt caggatcaaa gacagaatga tcaaaaaaat aagatctttc aaatacttca
    13981 acacttgaat tactccaggg tgctttgcga attttgcggt gcaaccgctt tttgtctttc
    14041 cacctcttct tccgtcacgt aattgctgcc gggcattttt ttggcgtact ctttgagttc
    14101 cgccaccagc ctggccatct ccagcggatt tttaaagcgc gagccgtcat ccgtaacaat
    14161 cgcggcggtg atcgtaatca aaggaaattt ctggacgttc ccctggcggt tccggccgat
    14221 gaagaatccc ttttgggcat cttccggccg gtagtagctc aggatgcgcc cggcaaaatc
    14281 aaccaccagc tttttgcaga tgctctcgac ctgcccgggg tcgccgatga ggacataatc
    14341 atcgcctccg atgtggccca gaaacatgtc cttcagtttc atttcatcga tatagccctg
    14401 gagaagaccg gccacttctt taatgacctc gcttccccat gcatacccgt actgatcggc
    14461 aaacggcttg aaattatcca ggtccacctg gcacagtgaa aaagatttcc ccttcgccag
    14521 caattgctca atccggtttt caatcgccag atttcccggc agaccggtga gcggactggc
    14581 atccaggttg agcgcttcca gcactttgag ccttttggtc atgtgggcga aagaattcgc
    14641 cagcgctccg atttcatcgt tgcgtctggc ctcgatccgg tgatccaaat tcccctcggc
    14701 aatcaggcgg gtggctgcct gcaactgttt caaaggcttg gaaatattgc gcgtaatgac
    14761 cagtgccagc gtaatgccca gaaccagact gagcaggctg agccccagcg tcatgtgcac
    14821 ggccccccgc ccctggctgg cgatccggtt catttcatta ttgatgtcct ggttcacgtt
    14881 tttttgaatg tcccgaagct gcatcgccat ggcatcgatc acagcccttc ccgtcgtatc
    14941 ggaaatcgcc tgggcttcct gcagctttcc ggtctgcacc agcagggctt cctccgaaaa
    15001 caagtccttc aggcgacggt gtaaaactgc catttcggaa agcgcgccct tcgagcgacg
    15061 gtccagcttc agccgccccg tctcggccag tttttcctgg aattcaacgc tgcgcttcca
    15121 gaaaatctgc tccagctccg gatccttcag aatcagatat ttcttttcgg cgctttcctg
    15181 ggccagcaga atgtccatga gctgcttgga attttccatc agcacaaagt ggtgatgggt
    15241 gattttgtac gcgccgccgc tcagcttctg cagattgaac aacgcgaagg cactggccag
    15301 gacggtcaga aacgccataa acagataacc aagcaacaat ttgcggctga tcgtgagact
    15361 cattttacca aaaacccgag aaggacattg gccgcgcgga ataaagcatc cggcaaaaac
    15421 cagttttctg caagtagcac aaacaaccgg ttttcgtaaa gaattattta tttttccggg
    15481 gcgtttgtct ctttttttcc gccccccgga catgccgaaa gctccttgcc acgggcggaa
    15541 agttttgtta aactgccgcc aaaacaggcc gccctttacg tcatctcagt acacccgcga
    15601 aggcaggctt cggcctggcc ttgaccatct gcaagaaagg aaaacgccgc catgatttac
    15661 aatgaggagt ttgaaaccct gccgcgcgaa gccttgaagg ccctgcaagt caaaaggctc
    15721 cagcaggttc tgcagcgcgt gtatcacacg gtcggatatt acaaaaagtc gatggacgcc
    15781 gctaaaatcg cgcccgagca tatccgttcc ctggacgatt tgaaaaagct ccccttcatc
    15841 acccgcgcag accttcgcaa caattatccc ttcggcctgt ttgccgtccc gatgagcaac
    15901 atcgtccgcc tgcacgcatc ctccggcacc tccggccgct cctccgtatt cagctatacc
    15961 cggcgcgaca tcgaaacctg gacggacctg atcgcccgat cgctggttgc cgccggactg
    16021 aacaagaacg atatcgtcca taacgccttg agctacggcc tgcttccggg cggactcggc
    16081 ctgcattacg gcattgaaaa aatcggcgcc agcgtcattc ccatgtccga cggcaacacc
    16141 aagcggcaaa tccttctcct gcaggatttt ggaccgaccg ttctgtgcgc cacgccgtcc
    16201 tatgccctgc atctggccga ggagggaaag gcaatgggcg tggatatgaa atcgctgcag
    16261 ctgcgcgtgg ggatcttcgg agcggaaatc tggagcgacg ccacccgcga cgccatcgaa
    16321 aacgccttca acatcaaagc cctcaacatc ttcggcctgt cggaaatgat gggtcccggc
    16381 atcgccatgg aatgtcttga aggccgccgc ggcatgcaca tctttgaaga tcattttatc
    16441 gtggaaacca tccatcctga aaccggcgac gtcctgcccg aaggggaaga aggcgagctg
    16501 gtctttacca gcctgactag ggaagcgttt ccgctggtcc gctaccgttc cggcgacatc
    16561 tcgcgcctga ttcccgagcc ctgccgctgc ggccgcacgc atatccgcat ggatcgcgtg
    16621 ttgaaaagaa gcgacgacat gctgaccatc cgcggcatca acatcttccc cgttcaggtg
    16681 gaagcgattc tcaaggagat tgagggcgtc gagccggatt accagctgat tattgacaag
    16741 gtcggagcgt tggacgccgt cgagctgcgc gtggaagtcg gggaaaaatt cttcagcgaa
    16801 tcgggcggcg tcaaagaact gcaaaaaatt gagaaacgaa tcgtcaagga catgaaggat
    16861 tacctgagca tctcgccgcg cgtcaaactg gtggccccgc agacgctgaa agaaagcggt
    16921 aaaaaggtaa tcgacaagcg caccatttga taaacacttg gaggaatcat acatgaaggt
    16981 agaacaaatc tcggtttttc tggaaaacaa acccggcaca ctggagcacg ccacacgcat
    17041 cctgaaggaa aacaacatca acatccgcac cctgtcactg gcggaaacag ccgatttcgg
    17101 cattttgcgc ctgatcgtca atgacgtgga aaaaaccaac aaggttctca aagaagccgg
    17161 cttccgcgtc agcaaaaccg tcgtcgtggc cgtggaagtc cccgatcagc cgggcgggct
    17221 gcacagcatc atggacgtct tgaacaaaga agccatcaac gtggaatatc tttacgcctt
    17281 tgtggaaaaa agcggccaga acgccgtcat catcttccgc ttcgacgccc cggaaaaagc
    17341 catcgaggtg ctcttgaaac acaaattcac cgtcgtgccg ggagcaaagc tgtacgagtt
    17401 ttgagaacgc agatggggga tgaacgacgg cgggattgat cccaagttca taaattaaca
    17461 gcagcatcct ttatctgtaa ccgcttaagc ttctgctgat cctttcgtaa gaggacatta
    17521 aatgcgacca ctattcacta ttcacgccgg ggagtttgtg gtaggcgact acatcgagcg
    17581 aaaatttcga aacgtaaatc tttgggtgcc atcccgtgat acaggcattg atcttttggt
    17641 tactgattca aataataaaa actctttatc cttgcaggtc aagttctcgc gtgatttcct
    17701 caccacacat atgtccgcta tttttcaaaa gcctttgagg gcatgcggtt ggtggtccct
    17761 gaatcgcgaa aaaatcgaaa aatcaaaagc agattattgg gtgtttgtat tgattggttt
    17821 cgagcacaga acaacagatt tcgtaatcat aaagccatcc gaccttctta ctcggctcct
    17881 tgcaattcac aataagagca aaactattca gagttatctt tgggttacag agaaaaatcg
    17941 ttgttgggag tcgcgttcct taaaacgtca agaacagcta gccattgcac aaggtcagta
    18001 ctcaaacatt gagcgtgact tttcaagata cctcaacaat tggagcccca tacaggcact
    18061 taatggaaag aaaataaccc tttaaccagt ctttgcaccc gatcacagcc caatgggatg
    18121 ctctgtgagc ttttcgttag tatgtttaca aagggggtct tttgagcctt ttttttcaac
    18181 acattttaga gctttatagt aatgcggaat atgccaccgc aagagaagaa ctgcgcaatt
    18241 ttgaaaaggc taataagcct ttcccggact gcatttataa agagattgac tatgcagaat
    18301 cgaataaata cagaaaaatc agggatgaga atgtatttcc catacgcttt ctatggttgt
    18361 ttgaacttaa agaacaaata gtgagtgggg tagttatgcc ccttgaaagg tcttattata
    18421 acgaagtctt attagcaaac t
//
LOCUS       DAOSIM010000016        17504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_3458_length_17504_cov_6.879764, whole genome shotgun sequence.
ACCESSION   DAOSIM010000016 DAOSIM010000000
VERSION     DAOSIM010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 17504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17504
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3458_length_17504_cov_6.879764"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PLR47_01600"
     CDS             complement(<1..841)
                     /locus_tag="PLR47_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11659.1"
                     /translation="MTANPPTSVRPDRPKFRDLLARPDIRARWEKVRKYFFLRESTYD
                     MTNRCNIRCDGCYYYEGDKPFAAENLSVPDWRKLMRAEKARGITYVVLAGAEPSLVPQ
                     LLEVCYAEMPLGCIATNGFKKIPEPVGYKIHISVWGNDETSEKTRGAKHLLQKQIENY
                     RNDPRAVFVYTFTRENIEEAQGVAAQLVAAGCKMTFNVFSSPVGYAGSLRHDADTLLK
                     TREMMLALLNRYPRQVLFSAYNAVAHTSDRGLHDLYGCSYPRRNPSQDIGLGRSFRQY
                     RTDL"
     gene            complement(862..1134)
                     /locus_tag="PLR47_01605"
     CDS             complement(862..1134)
                     /locus_tag="PLR47_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOG11660.1"
                     /translation="MPVNIELKAIHRRVAEIIIDELKLEDVTPDTFNPEMDLVDELGI
                     DSMDLATIALVLQDEYKIVIDEDDYPRLKTVRLICEYIEDKIATRH"
     gene            complement(1152..>1398)
                     /locus_tag="PLR47_01610"
     CDS             complement(1152..>1398)
                     /locus_tag="PLR47_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11661.1"
                     /translation="LPGIALLSAVYEAVLKDASRRGEPLRISGLKRVRFTGPVRPGEK
                     LSLVLAREIQNNEILFHFKVAVRENTVCSGLMSVAKV"
     assembly_gap    1399..1498
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1528..2352)
                     /locus_tag="PLR47_01615"
     CDS             complement(1528..2352)
                     /locus_tag="PLR47_01615"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOG11662.1"
                     /translation="MEEGKKRLSPALIAGGAALMAAFAVFFVNPLLSASVLSVYVGLC
                     VASCFLPQAHFLGPVISRGRTGQRVVALTFDDGPSEATTPKVLDLLDRHGAKAAFFVS
                     GANAARFPHLVGEMVRRGHSIGNHSMNHDPFLMLRGSRTLRREIVQAGEVLQGMGIDA
                     LAFRPPVGIVNPKLFPVLEALGLFCVTFNCRARDAGNWRIRNLSDRILKKVKSDDIIL
                     LHDRPPRGFEDPAVFWDELEKTLAGIKDKRLRIVPLSYLAGRDIMRLKSPGDPAFF"
     gene            complement(2487..3839)
                     /locus_tag="PLR47_01620"
     CDS             complement(2487..3839)
                     /locus_tag="PLR47_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid biosynthesis B12-binding/radical SAM
                     protein"
                     /protein_id="HOG11663.1"
                     /translation="MKKVLLISANRMKKPYPVYPLGLDYVAGALHSRYQTKILDMNHC
                     PSLGSLGEQLRAYEPDYIGFSIRNIDNTDLMNARGFLSDYRDMIGVIRRHSKAPLILG
                     GSGYTVFPLEFFHALGADYGIAGEGERLPVLLDALEQGRDASSVPGVITPDTPSIRYL
                     PWKGELHRVFDPDAEYAKFYVSYGGMLNLQTKRGCPFRCSYCTYPHIEGGKMRLLDPP
                     EVADQARRLQEAGARYIFVTDSAFNASVEHSLQVARAFRDRGITIPWGGFFAPTALPD
                     GYFRELAQAGLTHVEFGTESLSDTMLSNLRKPFAAADVFDAHRQAQTAGLYIAHYFLL
                     GGPGENRETLRETLEGADRLSRAVFFFFCGIRIYPHTALYDTAVREGQIQASQNLIEP
                     VFYRSPFLSGPEIIRTVEAHAAGRINWLIGAGEGKATRILPRLYERGHTGPLWEYLIA
                     "
     gene            complement(3898..4170)
                     /locus_tag="PLR47_01625"
     CDS             complement(3898..4170)
                     /locus_tag="PLR47_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOG11664.1"
                     /translation="MSTVEEVISELKGKIIATLGLLDVTPGDIKDDDPLVGGETGIDS
                     IDVLELVMMIEKDYGVKIDSKELGARVFASVRTLATFILQAKASKA"
     gene            complement(4232..4648)
                     /locus_tag="PLR47_01630"
     CDS             complement(4232..4648)
                     /locus_tag="PLR47_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11665.1"
                     /translation="MRLVLSILKKTALYFARKEYCRSAIEEKADLSAIKEKPTPKILV
                     GLVLIAFSYVIGLPAVVALGVIAVWIRKPLVVIIGGPLIYAVSTIVFIVGIRMAGDKY
                     FRVFSRWLVRVVLEKILGKELQTPSGNGPDNPCPGD"
     gene            4801..5232
                     /locus_tag="PLR47_01635"
                     /pseudo
     CDS             4801..5232
                     /locus_tag="PLR47_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            5347..5496
                     /locus_tag="PLR47_01640"
     CDS             5347..5496
                     /locus_tag="PLR47_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOG11666.1"
                     /translation="MCSICGYVYDPAEGDPDNGIAAGTRFEDVPDSWTCPVCGAEKKM
                     FEVDR"
     gene            5663..7786
                     /locus_tag="PLR47_01645"
     CDS             5663..7786
                     /locus_tag="PLR47_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOG11667.1"
                     /translation="MTDAIKQISLDVLVAMNTVVKNVRFYPLSSALVMNGMENLHQVF
                     LNYFKAYPSFILSESEKTLLVGDEPLTQKDHEKSYVASTLEILLKFNIKSMSLHRGLE
                     KRELLTLLSYLAKDPEKILDEGGLPELIEKNHLSHIRLDEKIFVAKGKNQQIVAGLEL
                     TDDQIMRYLGESHPELKGDRQKLLELTDSPEWLLRSFEESLSNLAAEKDVLPAVQFAE
                     KLTGILKRMDEVSDQMDPDTQKKIRENIDRAAAGIGEAVASEILLQHGEESASGALMR
                     HLVGRLLPDAGAAASEISKDADASGGNEAPEADSLPQIRENIERLLGDSAKGVLDQAV
                     MSSLTRTVKTLAAHKEQATVDTIMNRFLANMFAEDARVRDQAARALIEIIENLPDKEK
                     QAVLGGIAGKLLDWIRLETLATPAYRKICNFLRDHVNDLIWQDDFHAAIPILDFFNDV
                     RTGVTEKNDTIHEISVDFIDACASAERIAILFNFFKEGEPDKKRDAGEMLIRLGDAAL
                     NQLLDILKDHRDSNERVRIMRLIISAGRRAIPPVRDRLGKDQPWFYLRNIAYILSHIG
                     DESSAEAMQPLLLHENIKVRMEALKSISRTGKSQRCTLLLSVLPQVEHKFMINLVDAL
                     GNAKCAEAEPTLLILLENNPIRDKDLRATLQERICAALGSIGLPNSIPALSKIVGTKS
                     FLGISRYPENVKAAAARALASIQRR"
     gene            complement(7799..8089)
                     /locus_tag="PLR47_01650"
     CDS             complement(7799..8089)
                     /locus_tag="PLR47_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11668.1"
                     /translation="MKMFFMIYTEAMDERVTEAFKNAGFRSYTKMHGATGEGEESEPK
                     LGTHYWPGRNNTLLLGVPDEEVQKLVDLVRKFKQENPRAGLRAFTFPLEECV"
     gene            complement(8114..11239)
                     /locus_tag="PLR47_01655"
     CDS             complement(8114..11239)
                     /locus_tag="PLR47_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG11669.1"
                     /translation="MWLADTSVKRPVFATMVIMALVVLGVVSYPSIGVDLFPKIDFPI
                     VTITTTLKGASPDVMDVDVTDRIEGAVNTINGVKTITSTSYESMSRTTIEFVLERDID
                     QAVQDVREKVSGIRNKLPQDIEEPRIAKVDPDAQPILFLNLSGNRSVRDLSMYADEVL
                     KEQLQRISGVGDVIFYGLRLRQVRIWLDAAKMQAYQVAPSDVVLALKRENIELPGGRI
                     ETRTKEYTVRIKGEFAKIPDFNDLIISYYKGAPVRVRDIGRAEDGMEEKRSLSRFNRI
                     PAVGMAIQKQSGTNTVEVADRVKAEVAKINKTLPPGMKVNTAVDQSVFIVRSIDEVQF
                     HLIIGSIFAILAVFIFLKNIRTTLISAVALPVSVISTFALINAFGFTFNNMTMLALSL
                     SVGLLIDDAIIVIENIYRHVEEGMEPREAATFATSEIGLAVMATTFAIVGIFLPVAFM
                     KGIIGRFFLQFALTIIFAIMVSLLVSFTLTPMMASIFLKPHKKSIPNPLAAGGGGGGN
                     VQKPIWTRIGDRMEYFYAKLESSYRTVLEWTLGWRKTVLVCALMIFLLSLGLTAFIGK
                     EFNPPEDQGVFLIRMEAPIDYSVLQIEQYLGKTEAMMQEIPEIKSVFYVQGYGGYPNR
                     AMMMLNLTPKAERKRSQEDIKKIARTKLRTIPGLKVSAEDISVVGGGIRNVPIQYSIR
                     GQDLYALQKYAKKITAEFSKLPGIVDVDTSLEVGKPEFKVYIDRDRAAALGVDVASVA
                     EAINLLISGELDIARYKDELKGKRYDIRVRLNPEDRASAEGMQRIAVRARDGKLVELA
                     NVVKVEEGTGPSVINRADRQRAITVFASLEGKPLGQAKEELDAIAGRILPVDYMPKYY
                     GMADVMTESFGYLLFALLLGVIMAYMILAAQFESFIHPITVLLSLPLSFVGAFGALFL
                     TGKTLNIFSFIGLILLMGLVKKNAILLVDYTNVLRGRGLSRRDAILQAGPVRMRPILM
                     TTFAMVFGMLPIALGVGEGAETRAPMGISVIGGLLTSLVLTLIVVPSAYDIFDDWQEK
                     FKNRTRKTAEK"
     gene            complement(11226..12332)
                     /locus_tag="PLR47_01660"
     CDS             complement(11226..12332)
                     /locus_tag="PLR47_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG11670.1"
                     /translation="MRFLQYQNSGGQAARRLSALILLTLAVLTLASCQREKAAEPERL
                     VNVRIWPAETKRVEPYLETTGTLKAFEEVIVSSEVDGILRRIEVDEGSPVHAGMLLAE
                     INDTDYRLDRKRSDAALKQGRASLDNARAEYKRKEALYREELITKQQFDDVSTRVALA
                     EAELERIRATLSISQERLARTKIHSPLSGAVKEKKVSAGDYVRNGTPLFQLIRIHPLK
                     LNFTVSEKDVAALKIGQEVSFTVDAFSGKTFRGRVNLLYPNVEERTRTLQAEAVVPNA
                     DHLLKPGYFARVQIYTQAARDAVLIPVTALLYDGPVIRVFVVRGNQAQERIIKTGNKY
                     GEGVEILEGLKEKEQVVVVGQNNLSDGVKVHVAR"
     gene            complement(12371..13663)
                     /locus_tag="PLR47_01665"
     CDS             complement(12371..13663)
                     /locus_tag="PLR47_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG11671.1"
                     /translation="MMILWTALLLCSPTAFAQEYSLRDLYAEAVKNSEKIKYAEENLY
                     IAKMGKEKAWAVLMPRVTAFGTYNRFSEKKYGDPPAYGNVLIQPNESGNWGVRADESF
                     SLSIRELDALKISGQLISKSEFDLESTKSDFIFFVATAYYDALKAKKALEIAAANVER
                     LTQYRYFVEKRLKVGEVTRTALLRADGELSGAQSDYLRATNAFQLARAALVRVTGIEE
                     NFRLREEAPDFSAVDAFDRLRQTAFQMRPDLKSYDVQIQVSERQVKYARGAFWPYVGF
                     FAIYNGADQNPATSTLNRESVLAGVSLNFPFFEGGLRVAELKEARSRERQARLAYDDL
                     KKNVDIEIRGAWLDLETQRGTLKFLEDQLVFAHDNYNAVLRQYQNGLATSLDVMDANT
                     LLLTSERNTVDALYGCQLAHLRVRKSSGTLLRFVQEGY"
     gene            complement(13723..15060)
                     /locus_tag="PLR47_01670"
     CDS             complement(13723..15060)
                     /locus_tag="PLR47_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG11672.1"
                     /translation="MKTTCRGLRVFIFALSLMLCPAAVSAQALTLEDSIAVALQNSHV
                     LNIAKEGVKGAEAQKKEALTGFLPKFSTTYSYTRLNEDPNFYFPGFAGPPVIQGGPMT
                     TGTINNYNWGIEARQPLFAGGGILANYQAFKIAEDTALVEQTAKSLDVVQDVKIAYFN
                     ILRTQRLMETARQQVDMLAAHCDVAENFYRVGLIPKNDMLHAEVELANGRQTLVKAKN
                     GVESTKARLNTILKREVWTPLEVVDILDYQPLRQSFEECLAVARQRRPELKMSELRVE
                     QAGKMVRAAQSEFFPAVNLVGNYGRFGDTASLSGTQYKDTESWQVMAVASWNFWEWGK
                     TKYRVDAGKARENQALDQSRELNDQIALEIKNAFLALQEAESQIAVSKKVIEQAEENF
                     RIAEARYKERVARSTEVLDAQTLLTRAKAEYANALADYNIAHARLQRAMGVVR"
     gene            complement(15153..16220)
                     /gene="mnmA"
                     /locus_tag="PLR47_01675"
     CDS             complement(15153..16220)
                     /gene="mnmA"
                     /locus_tag="PLR47_01675"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOG11673.1"
                     /translation="MQGGAGRKILVAMSGGVDSSVAALLLREAGHEIAGATMLLGVHR
                     PGDAPGRFTDESAADAQRVCDQLGIPHRTFDFADLMENRVIARFIREYQSGRTPNPCV
                     DCNRHLKFGALFAKARSLGFDCLATGHYARIVKRQSRWALMRPKDESKDQTYFLYPIP
                     AGDLPSILFPLGALTKTEVRGMARRAALQTALREDSQDLCFVTAGNYRQLFEDRRLPV
                     VPGEIVNPAGQVLGRHRGIVSYTIGQRSGLGISAKTPLYVLGFDPAKNRVVVGKREEL
                     RSSGCIAGDLNMLADRFPEDVEAKIRYRKRPVRCRVIREGDRMKVVFEQAQEAVTPGQ
                     SVVFYAADEVLGGGVIEEVIR"
     gene            16435..>17504
                     /locus_tag="PLR47_01680"
     CDS             16435..>17504
                     /locus_tag="PLR47_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG11674.1"
                     /translation="MEIHFLLFVLLLAATAVISFAYAIWRGRNDQQILRSILQSSPIP
                     TFVISTNRKILYWNRALEGLSGIRAEEVLGTDQQWRAFYSSPRSCMADLIISKKIDHS
                     YSLYAGKVAKSKILKYAYEVTEFFPDLGEKGKWFRITATSIRDSSGKTFGAMETLEDI
                     TDQKTAEEELLKRTKLESLGTFADGVAKDFDSLLTAILRNVFLAKISASEEDKILENG
                     LAVAERAGLQAKELAHRLITFAQGGYPVKKIERIGGLIQETVEKTLAGSKVTCKPAIA
                     ADLWPVEVDALQIKQVIENMVTNAKEAMPEGGSMEVIAENTTTGTNIRTLGEGNYVRI
                     VIRDRGVGIPRENLSKIFDPYFT"
BASE COUNT         3861 a         5193 c         4679 g         3671 t
ORIGIN      
        1 tcaaatccgt ccggtactgg cggaaggagc ggcccaggcc gatatcctgc gacgggttgc
       61 ggcggggata cgaacagccg taaagatcgt gcagcccccg atcgctcgtg tgggccacgg
      121 cgttgtacgc ggaaaagagc acctgccggg gatagcggtt taaaagcgcc agcatcatct
      181 cccgggtttt caacagcgta tccgcgtcgt ggcgcaggga cccggcgtag ccgaccgggg
      241 aggaaaaaac attaaacgtc attttgcagc cggcagcgac cagttgggcg gccacaccct
      301 gcgcttcctc gatattttcc ctcgtgaagg tatagacgaa gacggcgcgg gggtcgtttc
      361 ggtaattctc gatctgcttt tggagaagat gttttgcgcc gcgcgttttt tcgctcgtct
      421 cgtcgttgcc ccagacggaa atatggattt tatagccgac cggctccggt atttttttaa
      481 atccgttggt ggcgatgcag cccagcggca tttcggcata gcagacctca agcagttggg
      541 ggaccaggga cggctccgcc cccgccagca ctacataggt gatgccgcgc gccttttccg
      601 cccgcatcag tttgcgccag tccgggacgg aaagattttc cgcggcaaag ggcttgtccc
      661 cttcgtaata gtagcatccg tcgcaccgga tgttgcagcg gttggtcatg tcgtaggtgg
      721 attcccgcag aaaaaaatat ttccgcacct tctcccatcg cgccctgatg tccggccggg
      781 ccagcaggtc tctaaatttc ggccggtcgg gtcgaacgga tgtcggggga ttcgcggtca
      841 tggaatatcg gtcttttgcc ttcaatgccg ggtggctatt ttatcctcga tatattcaca
      901 gatcagccgt acggttttca atcggggata atcgtcttcg tcgatgacaa ttttgtattc
      961 atcctgcaaa accagcgcga tcgttgccag atccatgctg tcaatgccca gttcgtccac
     1021 cagatccatc tccggattga aggtgtccgg cgtcacgtct tccagtttga gttcgtctat
     1081 gatgatttcg gccacacgcc gatgaatggc tttcaattca atgtttacgg gcatttcagc
     1141 ctcctttatc cctatacttt tgcaaccgac atcaggccgc tgcaaaccgt gttttcccga
     1201 accgccactt tgaagtgaaa cagaatttcg ttgttctgaa tttcacgggc aagcaccagg
     1261 gacagttttt cccccggccg gaccgggccg gtaaacctta cccgcttcaa cccggaaatc
     1321 ctgaggggct ctccccggcg ggacgcgtcc tttaaaaccg cctcgtagac ggcgctgagc
     1381 agagcgattc ccggcaggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat
     1501 tcccggcagg cgcaagcgaa aggaatttca gaaaaaagcc ggatcgccgg gggatttcag
     1561 ccgcatgatg tctctgcccg ccagatatga aagcggaacg atccgcagcc ttttgtcctt
     1621 gatgcctgcc agcgtttttt ccaattcgtc ccaaaaaacg gccgggtctt cgaaaccccg
     1681 gggcggccgg tcgtgcagaa ggataatatc gtcgcttttt acttttttga gaatcctgtc
     1741 ggaaagattc cgaatgcgcc aattacccgc atcccgcgcc cggcagttga aggtgacgca
     1801 aaacagcccc agcgcctcca gaacgggaaa aagcttcgga ttgacgatgc cgaccggcgg
     1861 gcggaatgcc aaggcgtcga tgcccatccc ttgaagaact tctccggcct gaacaatttc
     1921 acggcggagc gtgcggctcc ccctcagcat caaaaaagga tcatggttca tggaatgatt
     1981 gccgatgctg tgcccgcgcc ggaccatctc gcctaccaga tgcggaaagc gcgcggcgtt
     2041 ggcgccggac acgaaaaaag cggccttcgc gccgtggcgg tccagcagat ccagaacctt
     2101 cggcgtcgtg gcttcggaag gaccgtcgtc aaaagtaagg gcgacgaccc tttgccccgt
     2161 gcgtccccgg ctgatgaccg gccccagaaa gtgtgcttga ggtaaaaaac aggacgctac
     2221 gcacaacccc acatacacgg acaacacgga agccgaaagc aggggattga cgaaaaaaac
     2281 cgcaaacgcg gccatcaacg ccgcaccccc cgcaatcagg gcggggctta accgtttttt
     2341 tccttcttcc atgagaagcc tctccgggcg cgccgcttgg aacccgcgca aaacgggttg
     2401 aatgccccga cggcctgatc acctgaaggt tgcgcgagat ggcgcgaggt caaaccgacc
     2461 ctccccgcga aaaaaccgga ccgctgtcat gcgatgagat attcccacag cggccccgta
     2521 tgcccccgct cgtagagcct gggcaggatg cgtgtcgcct tgccctcacc agccccgatc
     2581 agccaattga tccttccggc ggcatgcgct tcaaccgtcc gaatgatttc aggcccggaa
     2641 aggaagggcg agcggtaaaa aaccggttcg atcagattct gcgacgcctg aatctggcct
     2701 tcgcgcacgg ccgtgtcgta cagggccgta tgcgggtaaa tgcggatgcc gcagaaaaag
     2761 aaaaaaaccg cccggctcag ccgatcggcg ccttcgaggg tctcccgcag cgtctcccgg
     2821 ttttcgccgg ggccgcccag caaaaaatag tgggcgatgt acagaccggc cgtttgcgcc
     2881 tgccggtggg catcaaagac atcggcggcg gcaaaaggct tcctcaggtt cgacagcatc
     2941 gtgtcggaca acgattccgt gccgaattcc acatgcgtga gtccggcttg cgccagttcg
     3001 cgaaaatagc cgtccggcaa ggcggtgggg gcaaaaaatc ctccccaggg aatggttatc
     3061 ccccgatccc tgaaggcccg ggcaacctga aggctgtgct cgacgctggc gttgaacgcg
     3121 gaatcggtca caaagatgta ccgggcgccg gcctcctgca gacggcgggc ctggtccgcg
     3181 acctccggcg ggtccaggag cctcatttta ccgccttcga tgtgggggta ggtgcagtag
     3241 ctgcatcgaa acgggcatcc cctcttcgtc tgcaaattca gcatgccgcc gtacgaaaca
     3301 taaaatttcg cgtattcggc atcggggtca aagacccggt gcagctctcc tttccaggga
     3361 agatagcgga tggacggagt atcgggagtg atcactcccg ggacagacga agcgtcccgg
     3421 ccctgctcca gcgcatcgag cagcacgggg agcctctccc cttctccggc gatgccgtag
     3481 tccgcgccga gcgcgtggaa gaattcaagg ggaaaaaccg tatagccgct cccccccaga
     3541 ataagcggcg ccttcgaatg ccgccggatg acgccgatca tgtcccggta gtcggaaagg
     3601 aaaccgcggg cattcatcag gtccgtgttg tcgatattgc ggatcgaaaa tccgatgtag
     3661 tccggttcgt acgcccgaag ctgttccccc aggcttccaa gcgacggaca gtgattcatg
     3721 tccagaattt tcgtttgata ccgggaatgc agggcgcccg ccacatagtc aagccccagc
     3781 ggataaaccg gatagggctt tttcatcctg ttggcggaaa tcagcaatac tttcttcatg
     3841 aaaacattct gctttgaatg gttgaaagaa cagggccgtt tccggccccg gcaggaatca
     3901 ggctttggaa gctttggcct gcagaatgaa cgtcgccagc gtgcggaccg aagcaaacac
     3961 cctggcgccc agttccttgc tgtcaatctt gacgccgtaa tccttctcaa tcatcatcac
     4021 cagctccagc acatcgatgg aatcaatgcc cgtctcgccg ccgaccagcg gatcatcatc
     4081 ctttatatcc ccgggggtta cgtccagcag ccccagcgtt gcaatgattt tccctttcag
     4141 ttcactgata acttcttcta ccgtactcat gcttcacctc gacaaattaa tgaaaacttt
     4201 ttcacggcgc caagccgcag ccccgcgcgc ttcaatctcc cgggcaaggg ttgtccgggc
     4261 cattcccgga cggcgtttgc agttccttcc ccagaatttt ttccagcacg acccggacaa
     4321 gccagcggga aaacacccgg aaatacttat cccccgccat tcggatcccg acaataaaaa
     4381 cgatcgtgga aacggcgtaa atcagcggcc cgccgatgat caccaccagc ggcttcctga
     4441 tccagaccgc gatgaccccc agggcaacga ccgccggcaa accgatcaca taactgaacg
     4501 cgatcaggac cagcccgacc aggattttag gcgtcggttt ttccttgatg gccgacagat
     4561 cggctttttc ctcgattgcc gacctgcaat attctttgcg ggcaaaataa agggccgttt
     4621 ttttgagaat gctcaaaacc agtctcaacc ggacagctta tttacaaacc tcgggcatca
     4681 tatctgcaat cggcaaagat ttgcaatatt tttttatccc tcataatttc atggcatgaa
     4741 aaggcaaatt cagctataag gtttgacgat aaatttatac ggaaaagagg aataaaaacc
     4801 atgaacagag cagccctcca ccaaatcagc tacggcgttt acatcgtcac gtccggcaaa
     4861 gacggcaaat tcaacggtca gatcgccaac tccatcgtcc aggtcacctc caggcccgcc
     4921 accctggcca tctgcatcaa caaggaaaac tacacgcacg ccctcatacg ggaaagcgga
     4981 aaattcgtcg tttccattct ctcggaggcc gccccgatgg ccttcatcgg tcttttcggc
     5041 tttaaaagcg gccgcgacgt ggataagctc aaaggcgtga aaagcaaaac cggcgcgacc
     5101 ggcgtgccca ttgttctgga tcatgccctg ggattcatcg aagccgaagt ggcaggtgag
     5161 ctggacggcg gcacccacac gattttcctt ggaaatgtgg tcgaagcgga cctggcgggg
     5221 gaaggcacgc cgatgacata cgcctactat cagaacgtca agggaggcaa atcccccaaa
     5281 tccgccccga cgtacgaagc cgacgcggcc gccgccaaac cgaaagctcc ggccgcggcc
     5341 cgctatgtgt gcagcatctg cggctacgtg tacgatcccg ccgagggcga ccccgacaac
     5401 ggcatcgccg ccggcacccg attcgaggac gtccccgatt cctggacatg cccggtatgc
     5461 ggcgcggaga aaaaaatgtt tgaagtggac cggtaaggtt caggcatcag gacaaagaac
     5521 aggcgccggc ccttcgttgc aggtgatgct tgacactttg ccgcattttt tatagggtta
     5581 ttttccttta aaattttatg acatacgatc aaccggatgc tgcccccggt cataaaatga
     5641 tgaacggcag gagaaaacaa tcttgacgga tgcaatcaag caaatcagcc tggatgtcct
     5701 tgtcgccatg aatacggtcg tcaagaacgt gcgcttctat ccgctcagca gcgccctcgt
     5761 catgaatgga atggaaaacc tgcaccaggt ttttctcaac tatttcaagg catatccatc
     5821 gtttatcctg tcggaatcgg aaaaaaccct gcttgtcggc gatgaacccc tgactcagaa
     5881 agaccatgaa aaatcctatg ttgcctcgac ccttgaaatt ctgctgaaat tcaacatcaa
     5941 aagcatgtcg ctgcacaggg ggctggaaaa gcgggaattg ctcactctgc tgtcctacct
     6001 tgcaaaagac ccggaaaaga tcctggacga gggcggcctg ccggagctta tcgagaagaa
     6061 tcatctatcg catatccgcc tggacgaaaa aatattcgtt gccaagggga aaaatcaaca
     6121 gattgtcgcc ggcctggaac tgaccgacga ccagatcatg cggtacctcg gcgaatccca
     6181 cccggaattg aaaggcgacc ggcagaagct tctggagctg acggacagcc cggagtggct
     6241 gcttcggtcc tttgaggaaa gcctttccaa cctggccgcc gagaaagacg ttctgcccgc
     6301 ggttcagttc gcggaaaagc tgaccggaat cctgaagcgg atggatgaag tatcggatca
     6361 gatggacccc gatacacaga aaaaaatcag ggagaatatc gaccgggccg cagcgggcat
     6421 cggagaggct gtcgcctcgg aaattctgct tcaacacggg gaagaatccg caagcggcgc
     6481 gctgatgcgg catctggtcg gcagactcct tccggacgca ggcgcggcgg catccgaaat
     6541 ctccaaggat gcggatgcat ccggcggcaa tgaagctccg gaggcggaca gcctgccgca
     6601 gattcgggaa aacatcgaac ggctgctggg cgacagcgca aaaggcgttc tggatcaggc
     6661 ggtcatgtcg tcgctcaccc ggacggtgaa aaccctggcg gctcacaagg agcaggcaac
     6721 ggtggacacc atcatgaacc gctttctggc caatatgttt gccgaagatg ccagagtgcg
     6781 ggaccaggcg gcgcgtgccc tgatcgaaat cattgaaaac ctgccggaca aggaaaagca
     6841 ggccgtcctc gggggaatcg ccggcaaact gctggattgg atcagactgg aaacgctggc
     6901 aacgcccgcc tatcggaaaa tctgcaattt tctcagggac cacgtaaatg atctgatctg
     6961 gcaggacgac tttcacgcgg cgattccgat tctggacttt ttcaacgatg tccggacggg
     7021 cgtgacagaa aaaaacgata cgatccacga aatatccgtt gacttcatcg acgcctgcgc
     7081 ctccgccgag cgcatcgcca tcctcttcaa ttttttcaag gaaggggagc cggataaaaa
     7141 gcgcgacgcg ggggagatgc tgatccggct cggagacgca gcgctgaacc agcttctgga
     7201 cattctcaag gaccaccggg acagcaatga gcgcgtccgc atcatgcggc tgatcatcag
     7261 cgccggccgg agggcgattc cgccggtccg cgaccgcctc ggcaaggatc agccctggtt
     7321 ttacctgcgg aacatcgcct atatcctgag ccacatcgga gatgaatcca gcgccgaagc
     7381 gatgcagccg ctgcttctgc acgaaaacat caaggtgcgg atggaggcgc tcaaaagcat
     7441 cagccgcacc gggaaaagcc agcgctgcac gctgcttctt tccgtcctgc cgcaggtgga
     7501 gcacaaattc atgatcaacc tcgttgacgc tctcggcaac gcaaagtgcg ccgaggcgga
     7561 gccgaccctg ctgatcctgc tggaaaacaa tcccatccgg gacaaagatc tgcgggcgac
     7621 cctgcaggaa agaatctgcg ccgctctggg cagcatcgga ctgcccaatt ccatccccgc
     7681 cctttccaaa atcgtcggga ccaaatcctt tctgggaatc agccgctacc cggaaaatgt
     7741 gaaagctgcg gccgcccggg cgctggcgtc catccaaagg cggtaaggag ctcccgcctc
     7801 agacacactc ctccagcgga aaggtgaacg ccctgaggcc cgcgcgcgga ttttcctgtt
     7861 tgaacttccg gaccagatcg acgagctttt gaacctcctc gtccggcacg cccagcaaaa
     7921 gggtattgtt ccttccaggc cagtagtgcg tgcccagctt cggctcgctt tcctcgcctt
     7981 cgccggtcgc gccgtgcatt ttggtgtagg atcgaaagcc cgcgttctta aaggcttccg
     8041 tgacccgctc atccatcgcc tcggtgtaaa tcataaaaaa cattttcatg cgccctcccg
     8101 aaacagccga tgtctatttt tccgcggttt ttcttgtccg gtttttgaat ttttcctgcc
     8161 agtcgtcaaa aatatcatag gcggacggaa ccacaatcag cgtcagcacc agagacgtca
     8221 gcagtccgcc aatcaccgat atccccattg gagcgcgagt ttccgccccc tctccgacgc
     8281 ccagcgcgat cggcagcatg ccgaaaacca tggcgaacgt cgtcatgaga atggggcgca
     8341 tccggacagg tcccgcctga aggatggcgt cgcgccgcga caggccccgg ccgcgcaaaa
     8401 cattcgtgta atccacgagt aaaatggcgt ttttcttgac cagacccatc agcagaatca
     8461 ggccgataaa gctgaagata ttgagcgttt tgccggtcag aaacagggcg ccgaaagcgc
     8521 cgacaaaaga caggggcagg gaaagcaaaa ctgtaatggg gtggataaag ctttcgaact
     8581 gcgcggccag aatcatatac gccatgatca cacccaaaag cagggcgaaa agcaggtaac
     8641 cgaaagattc cgtcatgacg tcagccattc cataatattt cggcatgtaa tccaccggca
     8701 ggatgcgtcc ggctatcgca tccagctctt cttttgcctg tcccaggggt tttccttcca
     8761 gcgaggcaaa cacggtgatg gccctttgac ggtcagcgcg gttgatgacg ctgggaccgg
     8821 tgccttcctc tactttaacc acattggcca gctcaacgag tttcccgtcg cgggcgcgca
     8881 cggcaatgcg ctgcattccc tccgcactgg cccgatcctc cggattgagt ctcacgcgga
     8941 tatcgtatcg ctttcccttg agttcatctt tatagcgggc gatatccagc tctccgctga
     9001 tcagcaaatt gatcgcctcg gcaacacttg ccacatcgac gcccagagcg gccgcacggt
     9061 cacggtcgat gtaaaccttg aattccggtt tgccgacctc cagcgacgta tccacatcca
     9121 cgatccccgg cagtttggaa aattccgccg tgatcttctt ggcatacttt tgcagggcat
     9181 agagatcctg tccgcgaata ctgtattgaa tcggcacatt tctgattccg cctccgacca
     9241 ccgaaatgtc ctccgcggat actttcagcc cgggaatcgt ccgcaatttc gttcgcgcga
     9301 ttttcttgat atcttcctga cttctcttcc gttcagcctt tggcgtgaga ttgagcatca
     9361 tcattgcgcg attggggtac ccgccatatc cctgaacata aaaaacagat ttgatttccg
     9421 gaatttcctg catcatggct tccgtttttc ccagatactg ctctatctgc aaaacggaat
     9481 aatcgatcgg cgcttccatg cgaatcagga agacgccctg atcttcaggc gggttgaatt
     9541 ccttcccaat aaaagccgta agccccaggc tcaaaagaaa gatcatcagg gcgcagacca
     9601 gcacggtttt gcgccagccc agcgtccatt ccaggaccgt ccggtaagac gattccagtt
     9661 ttgcataaaa atactccatg cgatcgccga ttcgggtcca gatgggtttc tgaacatttc
     9721 cgccgcctcc cccacccgcg gccaggggat tggggatgga ttttttatgc ggtttgagaa
     9781 agatcgatgc catcatgggg gtcagggtaa aggaaaccag cagcgaaacc atgatcgcaa
     9841 agatgatcgt caaggcgaac tggagaaaaa accggccgat aattcctttc atgaaagcaa
     9901 cgggcagaaa gatgccgaca atggcaaacg tcgtcgccat aacggccaga ccaatctccg
     9961 aggtggcaaa agtcgccgct tcccgcggtt ccatgccctc ttccacgtga cggtaaatat
    10021 tttcaatgac aatgatggcg tcgtcaatga gcaaccccac cgagagcgac agcgccagca
    10081 tggtcatgtt gttgaaggtg aagccgaagg cgttgatcag cgcaaaggtc gagatcacgg
    10141 aaacgggcaa agcgacggcg ctgattaaag tcgtgcggat gttcttcaga aaaataaaaa
    10201 cagccagaat ggcaaaaata ctgccgatga ttaaatggaa ctgcacctcg tcgatcgaac
    10261 gaacaatgaa aaccgactga tccaccgccg tattgacctt catccccgga ggcagcgttt
    10321 tgttgatttt tgccacttcc gctttcacgc ggtcggccac ctccaccgtg ttggtccccg
    10381 actgtttctg aatggccatg ccgacggccg gaatccggtt aaaacgcgac agggaccgtt
    10441 tttcttccat gccgtcttcc gcccggccga tatcccggac acggacaggc gcgcctttgt
    10501 agtaactgat gatcaggtcg ttgaaatcgg ggattttggc aaactcccct ttaatccgga
    10561 cggtatattc cttggtccgg gtttcaatcc gcccccccgg cagctcgata ttttcacgct
    10621 tcagggccag gaccacgtcg ctgggagcaa cctgataggc ctgcattttg gcggcatcaa
    10681 gccagatgcg cacctgccgt aagcgcagtc catagaaaat cacgtcgccc acgccgctga
    10741 tccgctgaag ctgttccttg agcacttcat cggcatacat ggacaaatcg cgaaccgagc
    10801 ggtttccgga aaggttcaga aacaaaatcg gctgagcgtc ggggtccacc ttggcaatcc
    10861 tcggttcctc aatatcctga ggcagtttgt tgcgaatccc ggacactttt tcgcgcacgt
    10921 cctgaacggc ctgatcgata tccctctcca gaacgaattc gatggtcgtg cgcgacatgc
    10981 tttcatagct cgtggacgta atggtcttga cgccgttgat ggtgttgacc gcgccttcaa
    11041 tgcggtccgt gacgtccaca tccatcacgt cggggctggc gcctttcagg gtcgtcgtaa
    11101 tggtgacaat gggaaaatcg attttcggaa aaagatcgac gccgatagac ggataactga
    11161 cgacgcccag gaccaccagg gccatgatca ccatggtggc gaagaccggg cgtttgacgg
    11221 aggtatcagc gagccacatg aaccttcacc ccatccgaaa gattgttctg gccgacgacg
    11281 accacctgct ctttttcctt gaggccttcc agaatttcca ccccttcgcc gtatttgttg
    11341 cccgtcttga tgattctttc ctgcgcttga ttgccgcgca ccacaaaaac gcggatcacg
    11401 gggccgtcat acaggagggc cgttacggga atcaagacgg catcccgcgc cgcctgcgtg
    11461 tagatttgaa cgcgggcgaa atatcccggc ttcaacagat gatcggcatt gggaacaacg
    11521 gcctccgcct gcagcgtgcg ggttctttcc tccacgttcg gatacagcag attcacccgg
    11581 ccgcgaaagg ttttgccgga aaaagcgtcg acggtgaagc tcacttcctg tccgattttc
    11641 aaggcggcaa cgtctttttc gctgacggta aaattgagct tcaatggatg tatcctgatc
    11701 agctgaaaaa gcggcgtgcc gttgcgcacg taatcgcccg cggacacttt cttttccttg
    11761 acggcgccgg aaagcggcga atggattttt gtccgcgcca gcctttcctg cgagatcgac
    11821 agtgttgccc ggattctttc cagctctgct tccgccagcg ccacccgcgt cgagacatcg
    11881 tcaaactgct gtttggtgat gagctcctcc cggtagaggg cttcttttct tttatactcc
    11941 gcacgggcgt tgtccagcga ggcccgcccc tgcttgagcg cggcatcgga ccgtttccgg
    12001 tccagccggt agtcggtatc attgatttcc gcaagcagca tgcccgcgtg gacgggcgac
    12061 ccctcatcca cttcgatccg cctgaggata ccgtccactt cggaactgac gatgacttcc
    12121 tcaaaagctt tgagtgtccc ggtcgtttcc agatagggct ctaccctttt cgtttcggca
    12181 ggccaaatgc gcacattcac cagcctttcc ggttcggccg ccttttccct ctggcaggac
    12241 gcgagcgtca aaaccgcgag cgtgagcagg atcagtgcgg acaaacgtcg cgccgcctgt
    12301 ccgccggaat tttgatattg taaaaaacgc atggttcgct ccccttcccg acccgacgcc
    12361 gccgctttcg tcaatagcct tcctgaacaa accgaagcag cgtgccgctt gattttctga
    12421 ccctcaaatg cgccagctga caaccgtaca aggcatccac cgtgttgcgc tccgacgtga
    12481 gcagcagcgt gttggcatcc atcacgtcga ggctggtcgc cagaccgttt tggtactgcc
    12541 tcaggacggc attgtagttg tcgtgggcaa aaaccagttg atcttccaga aatttcagcg
    12601 tgcctctctg ggtttccaga tccagccagg ctccgcgaat ctcgatgtcg acgtttttct
    12661 tcagatcatc atacgccagc ctcgcctgcc gctccctgga ccttgcctcc ttgagctcgg
    12721 ccactctcaa gcccccttca aaaaacggaa aattcaggga aacgccggcc agaacacttt
    12781 cccggttgag cgttgaagtt gcaggatttt gatcagcgcc gttgtaaatg gcaaaaaaac
    12841 cgacatacgg ccagaaagcg ccccgggcat atttgacctg ccgctcggag acctgaatct
    12901 gcacgtcgta gcttttcagg tccggcctca tttgaaaggc ggtctgccgc agacgatcga
    12961 acgcatcgac ggcggagaag tcgggcgcct cctctctgag ccggaagttt tcctcgattc
    13021 ccgtgacgcg gaccagggcc gcgcgggcga gctggaaggc gttggtggcc cgaagataat
    13081 cggactgcgc cccggacaat tcgccgtcgg cgcgcagcag agccgtacgg gtcacctcgc
    13141 ccaccttcag ccgtttctcg acaaaatagc ggtactgcgt cagacgctcc acgttggcgg
    13201 cggcgatttc cagcgctttt ttcgctttga gggcgtcgta ataggccgtc gccacgaaaa
    13261 aaataaaatc cgacttggtg ctttccagat caaactcgct tttggaaata agctgtccgg
    13321 agattttcaa ggcgtccagt tcgcggatgc tcagcgaaaa actctcgtcg gcgcggacgc
    13381 cccagttgcc tgattcgttt ggctgaatga gaacattgcc ataggcaggg ggatccccgt
    13441 attttttttc cgaaaagcga ttgtacgtgc caaacgcagt aactctgggc attaaaaccg
    13501 cccaagcctt ctccttaccc atcttcgcga tgtagaggtt ttcctcggca tacttgattt
    13561 tttcggaatt tttcacggcc tcggcataca ggtcccgcag gctgtattcc tgcgcgaacg
    13621 ccgtcgggga acacagcagg agcgcggtcc acaaaatcat catgtacaac ggcaattgct
    13681 taactcgtga atatcctttt tcgtcccaaa aacgttttgc ggtcaacgga caactcccat
    13741 ggctctttgc aggcgggcgt gagcaatatt gtaatcggcc agggcattgg catactcggc
    13801 cttggcgcgg gtcaagagcg tctgcgcgtc aagcacctcc gtggagcgcg ccacccgctc
    13861 cttgtagcgg gcttcggcga tgcggaaatt ctcttcggcc tgttcgatca ctttttttga
    13921 aacggcaatc tggctttcgg cttcctgcag cgccagaaag gcgttcttga tctccagggc
    13981 gatctggtcg ttgagctccc gggactgatc cagtgcctgg ttttccctgg ctttcccggc
    14041 gtccacacgg tatttcgttt tgccccactc ccagaaattc cagctggcca ccgccatgac
    14101 ctgccagctt tccgtgtcct tgtactgcgt gccggaaagc gatgcggtat cgccgaaccg
    14161 tccgtaattt ccgaccagat tgaccgccgg gaaaaactcg ctttgcgccg cgcggaccat
    14221 cttgcccgcc tgttcgaccc ggagctccga cattttaagc tccggacggc gctgccgggc
    14281 aacggccagg cactcttcaa acgattgacg caggggctga tagtcgagaa tatcgaccac
    14341 ctcgagaggc gtccaaactt ccctcttcaa gatcgtgttc aatcgcgcct tggtcgattc
    14401 gacgccgttt ttcgccttca ccagcgtctg ccttccgttg gccagctcca cttccgcgtg
    14461 cagcatgtcg tttttgggaa tcaggccgac ccggtaaaaa ttctcggcca catcgcagtg
    14521 ggcggccagc atgtccacct gctgccgggc cgtctccatc agcctttgcg tgcgcaaaat
    14581 attgaaatag gcgatcttga catcctggac gacgtccagg gatttggccg tctgctccac
    14641 cagcgccgta tcctcggcga ttttaaaggc ctggtagttg gccaggattc cgccgccggc
    14701 aaaaagcggc tgacgggcct cgatgcccca gttgtagttg ttgatggtcc cggtagtcat
    14761 gggccctccc tggataacgg gcggcccggc aaatccggga aagtagaagt tgggatcttc
    14821 attgaggcgc gtgtagctgt aagtggtgct gaacttcggc agaaaaccgg tcagggcctc
    14881 cttcttctgc gcctcggccc ccttgacccc ttctttggcg atgttcagca catggctgtt
    14941 ttgcagcgcc acggcgatgc tatcctccag ggtcagcgcc tgggcggata cggcagcggg
    15001 gcaaagcatc agcgagaggg caaaaataaa gacccgcagg ccccggcatg ttgtcttcat
    15061 gtttggctcc attttgacat tggtgcgcac tgtttagtat attttccgca aaaaataaac
    15121 gttaaaataa tccttcggca aacccccggt cattaccgga taacctcttc gatgacgccg
    15181 ccgcccagca cttcatcggc cgcgtaaaaa acgacggact ggcccggagt caccgcttcc
    15241 tgggcctgct cgaagacaac cttcatcctg tcgccttccc tgatgacgcg gcagcgcacc
    15301 ggccttttcc ggtagcggat tttcgcctcc acgtcttcgg gaaaccggtc ggcaagcata
    15361 ttcaggtcac cggcgatgca gcccgaagac cggagttcct cccgcttccc gaccaccacc
    15421 cggtttttcg ccgggtcaaa tcccagaacg taaagcggcg tcttcgcgct gatgcccagg
    15481 ccgctgcgct gtccgatggt gtaggagaca atcccccggt ggcgcccgag aacctgcccc
    15541 gccggattca caatctcgcc gggaaccacc ggcaggcgcc ggtcttcaaa caattgccgg
    15601 taattccccg ccgtcacgaa gcaaaggtcc tggctgtcct cgcggagggc cgtttgcaag
    15661 gcggcccgcc ttgccatccc acgaacctcc gttttggtca gggcgcccag cggaaaaaga
    15721 atcgacggca gatccccggc aggaatcgga tacaaaaagt aggtctggtc cttggactca
    15781 tcctttggcc gcatcagcgc ccagcggctt tgccgcttta cgattcttgc gtaatggccg
    15841 gtcgccaggc aatcaaaacc cagactgcgg gctttcgcga agagcgcgcc gaatttcaga
    15901 tggcggttgc aatccacgca gggattgggc gtccgtccgc tttggtattc gcggatgaat
    15961 ctggcaatca cccggttttc catcaagtcg gcaaaatcaa aggtccggtg gggaatccca
    16021 agctgatcgc agactctttg ggcgtcggcg gcggactcat ccgtaaaacg cccgggggcg
    16081 tcccccggcc ggtgaacgcc cagcagcatc gtcgccccgg caatctcatg gcccgcctcc
    16141 cgaagcagca gcgcagcgac cgacgaatcc actccgccgc tcatggccac cagaattttt
    16201 cttcccgcac cgccctgcat gccgcgcctt tcaaaaagta gtttcccctt ttccacaatc
    16261 ttttgccgct ttgcaacttt tttttccgtg ccggcttttc caatcttgca atttcaaggc
    16321 aaccttgcta ttcagattcc accaaattcc gagggtgacc gtgaggcatt tttcccggcc
    16381 gcagggcggc cggatcgatc ttcacggaaa gattgggaaa accgggtgcc tggaatggag
    16441 atccattttc ttctttttgt cctgcttctg gcggcaaccg ccgtcatcag cttcgcctac
    16501 gcgatctgga ggggacgcaa cgaccagcag atcctccgca gcattctgca gagttcgccg
    16561 atccctacct ttgtcatctc caccaaccgt aaaatcctct actggaaccg tgcgctggag
    16621 gggctctcgg gcatccgcgc ggaagaagtc ctgggcaccg accagcaatg gcgcgcgttt
    16681 tattcttcgc cgcggtcctg catggcggac ctgatcatca gcaaaaagat cgatcactcc
    16741 tactcgcttt acgccggaaa agtcgccaag tccaaaatcc tgaagtatgc ctatgaagtg
    16801 acggagtttt tcccggatct gggggaaaag ggaaaatggt tccggatcac cgcaacgagc
    16861 atccgcgatt ccagcggcaa aacgttcggc gccatggaaa ccctggaaga catcaccgac
    16921 cagaagacgg cggaggaaga actgctcaaa aggaccaagc ttgaatccct gggaacgttt
    16981 gccgacggcg tcgccaagga ctttgacagt cttctgaccg ccattttgcg caatgtcttt
    17041 ctggcgaaaa tttccgcaag cgaagaggat aaaattctgg aaaacggcct ggccgtcgcg
    17101 gagcgcgccg gcctgcaggc caaggaactg gcccatcggc tcatcacctt tgcccagggc
    17161 ggctaccccg tcaagaaaat tgaaagaatc ggcgggttga ttcaagagac cgtcgagaaa
    17221 accctggccg ggtccaaggt aacctgcaag cccgcgatcg ccgccgacct ctggcccgtg
    17281 gaagtggacg ccctgcagat caaacaggtg attgaaaaca tggtcaccaa cgcgaaggag
    17341 gccatgccgg aaggcggctc catggaagtg atcgcagaaa acaccacgac cggcaccaac
    17401 atccggacgc tcggcgaggg caactatgtc cggattgtca ttcgggacag gggcgtcggc
    17461 atcccccggg agaacctctc caaaattttc gatccgtatt ttac
//
LOCUS       DAOSIM010000017        17210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_3524_length_17210_cov_8.099738, whole genome shotgun sequence.
ACCESSION   DAOSIM010000017 DAOSIM010000000
VERSION     DAOSIM010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 17210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17210
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3524_length_17210_cov_8.099738"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2041)
                     /locus_tag="PLR47_01685"
     CDS             complement(<1..2041)
                     /locus_tag="PLR47_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOG11675.1"
                     /translation="MKKRAFLLSVFVCLLFVSGVLAQTASPLLEEGIRQYQDENYEEA
                     IEILEKASLEDPPSALAAYFLGMAYKQTMDYARALIHLEKALKLKPAVKEALVEYVSL
                     LYRQDKLAEARKWIDVGKKENIDPANLFFLEGLILMKEKKPKKAIASFEKAKSLDPKL
                     AQSADYQIGLCYMQDRKYKQAKERFRATVAYDSTTDLAAYARHYQEIAENSQFYTRPL
                     RLTLGLQGGYDSNVITKPKEDALSGDSRDPATGMLSPLLRVEYVPRFETSWLLYAMYS
                     HGQTLHQRFTHARDSLVDSVAVMPGYNFGRVSVSLMGAYTNYLMKTDPDPVPDENAGL
                     KRYLDYFTVGPVVRWMVSPAHIAEGFAGYDKKNYYNQPMLSENNNRDNEGYRAYLSWS
                     WFYMDNGLLNVRYDITREAANGAYWDALLQTQEIVNGAEPGIMPFPQRRTDDNPFKGN
                     EAGFYLSKTRMSRYTACPRSYRLAYQLNIKPIKTPVELLIGKSTHKLIAESFLARAAN
                     MIPDLTGSLDAFWTDEMKAPATRTEMENAKREAGRYACLFIKEVDLKPLAVEKEFSIP
                     VVNYENGDILPVPLVGIIDLVDRPNGSASSPQGGTPRALEIKTRSRKGDDLQPRVSLE
                     LTCYAYWIKSQFLSDINEDVNINKIPVGYLNIIKTKTPSIQKQEGFRTIRDFIDLF"
     gene            complement(2183..3103)
                     /locus_tag="PLR47_01690"
     CDS             complement(2183..3103)
                     /locus_tag="PLR47_01690"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG11676.1"
                     /translation="MKTSLRFPWPIDLARFFWQRKIRGRSIPLLASFKVTYRCNLACL
                     ACPFHLRVDEEGAHMRWETAVKSLDTLSRLGTRIVVFEGGEPFLWQDGDRRLTDLVHH
                     ARRRFLRVAVTTNGTLPLTIASDILWVSLDGLKQTHDELRSGSFDRVLTNLKAANHPR
                     LFVHCTLNRRNWRDIEAIAGLVRGLPAVRGMTVQFFYPYGQGEEDLALSPDERRAAVD
                     RLLALKKQGFPVLNSSSRLRDMAHNRWRCHDDILINVDPDGSITAGCYVKSRGQVNCD
                     ACGFTPVAEASGALDLVPGSIRAGFRLFLK"
     gene            complement(3141..4967)
                     /locus_tag="PLR47_01695"
     CDS             complement(3141..4967)
                     /locus_tag="PLR47_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOG11677.1"
                     /translation="MSVSEHPYPEMYDPPTVTGIFMHQARRFGDKTMSLVKRAGRWDA
                     ITWRQARDLLPKGVMGLFALGFQKGDAVGIASRTRREWSGIDLAVLSAGGVTVALYPS
                     ASLWEMTHIVSHSGLRICFAEDDAMLDRLLKVQAETGLPEKIVLFETARDKLPDGVIT
                     RDAYLERGECLHWSDPSRFEAIWRGVKPSDLATIAYTSGTTGPPKGAMITQANLYYTT
                     IHATKMHHYEENDFGIAFLPLTHMLQRMTVYAALHLGIVGAYAESIDKLIDNFQELHP
                     TVQVSVPQIFERIYNRIHQMTQASSPAKRRIFRWAVDVGMQAARLQREKRPLPFGPAI
                     RLRLADRLVFRKIRAIFGGRVKYLMCGGAPMPLYLLEFFHAVGLLILEGYGLTETVAP
                     VAVNRAECFKFGTVGQLIPGMEGKLAPDGELLLRGKGLFQGYYKDPEATARAIDAEGW
                     FHTGDIAEIDGEGFIRITDRKKDLIVTAGGKNIAPQNIEGLIRMMPLVDHVLVCGDRR
                     KYLTALITLDRLELQAFAGREGLADAPADVLAGHPAVQKAVADHIQAVNVRLAPYEQI
                     KRYKILPHEFSEASGELTPTLKMRRREIMERYARDIETLYQG"
     gene            complement(5259..7088)
                     /locus_tag="PLR47_01700"
     CDS             complement(5259..7088)
                     /locus_tag="PLR47_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11678.1"
                     /translation="MEARNAANPRWTFYALLIFLSVLWGCYGSGEPGDGSVTLDGPSV
                     TLESTYVASVATPGYRERAVMSQGQGEYAGVDEWGNSDPIPAGARLANGATSNLFSLD
                     FSDRSTVAYSNYFTSEATLRDAMSGDWLDAAFFNGRVEVGPWRAEGATGHFTYRRFGA
                     VYEVVRPLPVAYSVCRNNPQMDTAGAGGAPQYFAPFIRQDLIRLGYVRIVDAVDSTEA
                     SRTSTLARYTALYGRTAGADELAALAFADYTAFVALRTPPEQARAEALRVESTALRTE
                     KKRSDSAFSATAFAGDVTIPAGRRIDGFARVSLAAPRDIRLSGTIWGADQVFSSAGGH
                     CSVDEGGLVEVWTDRNRAGDVALSLACGTIEVGGRVQTQAAPWSSWSQGVPGQISLAS
                     ANDEPSETGISIAGSGQVLALLQTAQTDASLSAVRITSPGSGIVIDGPGYGFNVVADK
                     GAVHIENSGANGSVTILGRAAVSADVVRAGAPGDYGTLSISAGANLDANTLVKLFGGL
                     GSGGKVVFRGAGDVILTSGPGESPIVISADAVEVETATRLVTRGWIKTGSVWSRTAVA
                     ADVYCNRCNWSQASGGDSAYGLEGTWTTPPRRAGPPPADRSFD"
     gene            7199..8452
                     /locus_tag="PLR47_01705"
     CDS             7199..8452
                     /locus_tag="PLR47_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOG11679.1"
                     /translation="MAQNMNLVSSASPDDPSPRPAPPAADAFPETTRIQRIRLLKQIG
                     LYPFFVPSSGQVGPRMLMDGHDTIMLGSNNYMGLANHPEMIQAAVEATRKYGTSCTGS
                     RFLNGTLDIHNELEEKLSAFFGKDAAICFSTGMQANLGVISGITDKDDHIVSDEKNHA
                     SIIDGCRLSYAKTHVYKHDDMESLRRTLRALPKNGLRWIVSDGVFSMEGSVANIPEIV
                     ALAKEFNARIIIDDAHGIGYMGDHGEGCCGHFNLMDHIDILTGTFSKSFASLGGFCAG
                     RADLMDHLKHHARSLIFSASMPPAMVACAITALDLFQREKQLRKQVMVNASYLRIGVQ
                     TLGFRTVAHSIPTAIVPIMVGENLATYKLHKTLLACGVYTNPVVPPASAEGLLRLSVM
                     ATHTEDDLTDALNILHRVGREAGLI"
     gene            complement(8463..10568)
                     /locus_tag="PLR47_01710"
     CDS             complement(8463..10568)
                     /locus_tag="PLR47_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG11680.1"
                     /translation="MKAPKTSYRQGHRALIRAAGVLLALACMLWAAGAVRADDAQTIK
                     EQGDRYAREGDFTRAAQAYEDALSGPVDFGDDERLDMATVMAWGGKLGRSRRELTALL
                     EKNPQNAKARVQRGRVFFWMGEMDQASRDAQEALKLAPADRDATLLSADVARVQGDQE
                     KAVDVYQSVLTAGEHFEARVGLAYAYLAQGKVVLARQSAEKLSPELPYQRQEAATLQL
                     ALAEAEKPRTPSPDELARLAAREGNRLAEAGRHQDASEEYAKALSLSGSFTADEQLRM
                     ASVMSWAGNLAAARERLTKILTDHPEMSAARIHLARVLLWSGEYDAALREIDRVLALE
                     PSHREALLVRASGLRMTRNYRPAVALYSQLVLKQDDYDAREGLTYSYLQSGDRVAADQ
                     SLPLLKPAFAYEEKSLGELKELRDITFNPSVAPGFTFYSDSDQNQVWRFFAAGSVWLK
                     NWKTTVDYTRTDGRDASTTGSSDRLFVSTYSRMPFYGGVGGGVGLMDSGRGVAWSLRG
                     DVDIPNGSVGARVGVDSVSDTAGVIEHHVKALTAGLSGVYRATDRITLLAGYHYRDYS
                     DDNSAHDVMGQAGGLIWRRPAAVAAGYRIRYMNFRRQSGHGYFDPQNFIAHVLFVNAS
                     FERGRFYGYAEPYGGYQSFTRNEEGNYNMIIGGAGLLGYRFSKHAALEASAEGGNSAV
                     GASGAYNYYQLGARLILTY"
     gene            complement(10568..11347)
                     /locus_tag="PLR47_01715"
     CDS             complement(10568..11347)
                     /locus_tag="PLR47_01715"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOG11681.1"
                     /translation="MTQDLTRRAFLQFLAAVLILPETPRAAFAAAKTVAIPVLLYHDI
                     SPEHTDDYTLPPALFAAQMEWLCAEGYRAVSFADLASSALPERACIITFDDGYASFIP
                     YALPFLQAYGFKATINIIGQHVASHIKDGIIRPMLAWDEYRYLAATGLVDFGCHTERL
                     HVYRHQGAAGVADSVLLEDLAQFQKNFVREMGKRCDILAWPYGFYTEKSVAAARKAGF
                     RYLLTSKRGVYLRTDAPWDIPRNNITPDDNLLTFPSIFEVS"
     gene            complement(11344..13746)
                     /locus_tag="PLR47_01720"
     CDS             complement(11344..13746)
                     /locus_tag="PLR47_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11682.1"
                     /translation="MQLEKLGRLALEKGDYQEALNIFRRALDDDESVEGWMGLARAFL
                     GLDETPAARWAAHKGLAYSPGHQDILKLARRIEERMKAPRPVHPSQPDVRFRVGKAGF
                     EKRVGGVWRPFFVKGINLGLAAPGTYPGEYPIGKTQYRRWFGQMANAGFNTVRVYAIQ
                     SPSFYEALAEANDGQNALYLIQGIWIEPPDDGDFSSEAFLGYTRRQITEAIDALFGRI
                     TLPERQGQPHGVYTRDVSPQVAGIIFGREWEGCPIGKYNRLRSAEGSVFNGAFLSGAR
                     LSPFERWICRILDELLVYEERKYGVTHPVSTVCWPTLDPLAHPSESLYEDGLRWQGLA
                     VKASECNENEDAETLDTAKIAARKGSGFFATYHAYPYYPDFMNNDYLDREKPYSAYLS
                     QLKAHHKTQPILIAEYGVPSSREVSHWQRQGWHHGGHDEDAQGRINGEMLKAIAEAGL
                     AGGALFSWFDEWFKRNWLFVNYERPAERNALWFNLQDAEQCYGLVGAYPGYPGKKVTL
                     SGDAAQWQDATVLYRKDDQPAHCFGDGADGTRRLRSLAVQHDEGFLYLALTLGDPVDF
                     NRGHYAIGIDTCDPATGEFRLPFRLNAQSPVGLKFLVHLSGVQTSRMLICASYDKYLN
                     DPYQELWPRSSREGAWVSMHHKTNDRRISKNRDRVYPARVFPLSALRHGSLIPGDRLY
                     DSLADFHVSGNLIELRLAWGLLMVTDPSSRAVYWKQGEKWTRQTEGLRFVAYSFKPSG
                     RGWTAEITGKSDNATDRLPGVMEPRLIRPYAWPTWNVPLFHFYEKKSLAVYRRYLKEI
                     VP"
     gene            complement(13736..15154)
                     /locus_tag="PLR47_01725"
     CDS             complement(13736..15154)
                     /locus_tag="PLR47_01725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG11683.1"
                     /translation="MRAMLIAAIVGFNYFIGFFYGFVNLIYTLLIFVAVGSILIHIRK
                     IKYLPLLENRFSPEVPPVSVIIPAYNEGRILFRTIQSILDMNYPNCEVIVVNDGSRDD
                     TLLQLIGQYDLKQVDLVYRSLLKTKPIRGFYYNRNIPNLLVVDKEQGGKSDALNCGIN
                     ASRSPYVCSVDADTLMEPDALLRLMTAVMESPTPVVACGGVVRVINGVTIDGGRITKI
                     GLPSRWLGIFQIVEYLRGFLFGRAGLDRLGGTLILSGAFSMFQKQAVIDAGGFAINNV
                     CEDMEIIVRIHKQHLLAKKPYAVRFITDPICWTEVPETMKQLAGQRRRWHLGLIQTMM
                     KYKSMVFNPRYGKVGMLTMPYYFFAEMLSPFIEVLGYVAMVGAYVFNLINFNLFFLFI
                     FLAIFYGVFLSVVSIFLEEITYHRYPDWEHLFILLVFGVLENFGYRQINSFWRFQAFF
                     QYLFGKRRWEYHRQKETKPHAA"
     gene            complement(15151..16557)
                     /locus_tag="PLR47_01730"
     CDS             complement(15151..16557)
                     /locus_tag="PLR47_01730"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOG11684.1"
                     /translation="MAVTTEIADATLNIGSGGVNAFAADGSARPPAKQNENASSLSGS
                     VLDMFKGMERMEIVLLVLIFIIIGFCLLFFLLAVLRRLYNAHRYAVLDNVRSRQMFEL
                     KHYMQNKASLEQYHKRFAATPESTEWVALEYNLFDLMGAEATRTTAARLFDRLGYVDY
                     YIGVLKGRKVIARAQAVSKLGRMANPRAAKPVLEMLQTDHADIASVAVRALGKIGEGP
                     ELLALLERLPGLLQKNLVTQKTIDASLVAAGPRILPTLLTYARTCTDARLTASLLEVM
                     YDFPATREIYEFAVSHLGNDDAEVRAKALKLIAPCEEKFGTLHDDAPVRLLSDPVWYV
                     RLQAVRTLGRRRDAKNAAAIAVLMRDERWQVRNEAALALTLLGRDAVDAFRTLLMSDD
                     RYARESVCEEMEKSGFVEILLDLLGSGREGESAKAHEILTMMVSCGFVSPLKQYLVDT
                     DDPERMRNIQSFLTGAAR"
     gene            16761..16997
                     /locus_tag="PLR47_01735"
     CDS             16761..16997
                     /locus_tag="PLR47_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11685.1"
                     /translation="MSGKNSSGMMLAVKVSLASNVLLFIIKAITLFIVDSLAVAADLG
                     ISAIALCVSGILYYAVKMSDKPLIRTSFLWAEVY"
BASE COUNT         3666 a         5173 c         4869 g         3502 t
ORIGIN      
        1 caaagaggtc gatgaagtcc cggatggtgc ggaaaccttc ctgcttctgg atggatggtg
       61 tttttgtctt gatgatgttc aggtaaccga cggggatttt gttgatattc acatcttcgt
      121 tgatatcgct taaaaactgg gacttgatcc agtaggcgta acaggtaagt tccagggaca
      181 ccctgggttg aaggtcatct cctttgcggg agcgcgtctt gatctccagg gcgcggggtg
      241 tgccgccttg tggtgagctt gccgaaccat tcggcctgtc aacgaggtcg atgatcccga
      301 ccagcggcac tggcagaatg tcgccatttt cgtaattgac gacggggatg gaaaattcct
      361 tttcgacggc caggggcttc agatcgactt ccttgatgaa caggcacgca tagcgtccgg
      421 cctcgcgttt cgcattttcc atttcggtac gcgttgccgg cgccttcatc tcatccgtcc
      481 agaaagcatc cagcgagccg gtcagatcgg gaatcatgtt ggccgccctg gccaggaacg
      541 attcggcaat gagcttgtgg gtagacttgc cgatcaggag ctcaaccggc gttttgatgg
      601 gtttgatgtt cagttgatag gccagccggt aactgcgagg gcaggctgtg tagcgggaca
      661 tccgggtttt ggagaggtaa aagcctgcct catttccctt gaatgggttg tcatccgtcc
      721 ggcgctgcgg aaaaggcatt atccccggct ctgcaccatt tacaatttcc tgtgtttgca
      781 acagcgcgtc ccagtaggcc ccgttggccg cctcccgggt gatgtcataa cggacattga
      841 gcaatccgtt gtccatgtaa aaccacgacc agctcagata ggcccgataa ccttcattgt
      901 cgcgattgtt gttttccgaa agcatcggct ggttataata attcttcttg tcgtagcccg
      961 caaatccctc ggcgatgtgc gcggggctga ccatccacct aacgaccgga ccgacggtaa
     1021 aataatccag atagcgtttc aatccggcgt tttcatcggg aacggggtcc gggtccgtct
     1081 tcatcaaata attcgtgtag gcgcccatga ggcttacgga cacccggcca aaattgtagc
     1141 cgggcatgac ggccacggaa tcgacaagtg aatcgcgcgc gtgggtaaag cgctgatgca
     1201 gggtttgtcc atgggaatac atggcgtaaa gaagccagga cgtctcgaag cgcggcacgt
     1261 attccaccct caaaagcggc gagagcatgc ccgtcgccgg atcccgggaa tcgccggaca
     1321 aagcgtcttc cttcggtttg gtaatgacgt tggaatcata cccgccttgc agccccagcg
     1381 tcaaacgaag cggccgggtg taaaactgac tattttcagc aatctcctga taatggcgcg
     1441 cataggcggc cagatccgtc gtggaatcgt aggccaccgt cgcccggaaa cgctctttgg
     1501 cctgtttgta ctttctgtcc tgcatgtagc acaaaccgat ctggtagtcg gcgctctgcg
     1561 cgagcttcgg atccagcgac ttcgcttttt caaatgacgc gatggctttt ttgggcttct
     1621 tttccttcat caggatcagc ccttccagaa agaaaagatt ggccggatcg atgttttcct
     1681 ttttcccgac atcaatccac ttgcgggctt cggccagttt gtcctgacgg taaagcagac
     1741 tgacatattc caccagcgct tctttcacgg ccggtttcag tttcagcgct ttttccagat
     1801 ggatcagcgc cctggcataa tccatcgtct gcttgtaggc catacccaga aagtaggccg
     1861 caagggcaga cggggggtcc tccaggctcg ctttttccag gatttcaatg gcttcttcgt
     1921 aattttcgtc ctgatactgc ctgatgcctt cttccagaag cggacttgcc gtctgggcca
     1981 atacaccgga aacaaacagc aggcacacaa aaacgcttaa caaaaaagcc ctttttttca
     2041 tgaaaaagac tccattcttt aagacatatt attaaagata ttgaaaactt actgactacg
     2101 aagcagcgat gtcccatccg ttggcaaaag tcagataaga tatgatttat tatcgtcgaa
     2161 atcggcaaaa acattagccg gattatttca aaaaaagcct aaagcccgcc cggatcgaac
     2221 cgggaacaag gtccagcgca ccggaggctt cggccaccgg cgtgaagccg caggcgtcgc
     2281 agttgacttg tccgcggctt ttcacatagc agccggcggt gatcgagccg tccgggtcca
     2341 cattgatcag gatgtcgtcg tggcagcgcc agcggttgtg cgccatatcg cgcagacgcg
     2401 acgacgaatt taaaaccgga aatccctgtt tcttcagagc cagcaaccgg tccacggcgg
     2461 cgcggcgctc gtcgggtgaa agagccaggt cctcctcgcc ctgaccgtag ggatagaaaa
     2521 actgcacggt catgccccgc acggcaggca ggcctcgcac cagccccgcg atggcctcga
     2581 tgtcgcgcca gttgcggcgg ttgagcgtgc agtgcacaaa cagccgggga tgattggcgg
     2641 ctttgagatt ggtaaggacg cggtcgaacg acccgctgcg caattcatcg tgcgtctgtt
     2701 tcaggccgtc gaggctcacc cagagaatat ccgatgcgat cgtaagaggc agcgtgccgt
     2761 tggtggtcac ggcgacgcgc agaaagcggc ggcgcgcgtg gtggaccaga tcggttagcc
     2821 gccggtcgcc gtcctgccac aaaaacggtt cgccgccttc aaagaccacg atgcgcgttc
     2881 cgaggcggga aagcgtgtcg agcgatttta cggccgtctc ccagcgcatg tgcgcgcctt
     2941 cttcgtccac gcgcaaatga aaggggcagg caaggcaggc gagattgcag cggtaggtca
     3001 ccttgaagct cgccaaaagc ggaatgctcc gtccgcggat tttacgctgc caaaaaaacc
     3061 gcgccagatc gatcggccag ggaaaacgga ggctggtctt catgtgacat ccctcttttt
     3121 catattccac cagcccctta ttatccctga tagagggttt caatgtcgcg ggcgtagcgc
     3181 tccataatct cacgccggcg cattttcaag gtcggcgtca gttcgcccga cgcttcgctg
     3241 aattcatgag gaaggatctt gtagcgtttg atctgctcat agggcgccag acggacattt
     3301 acagcctgta tatggtcggc gacggccttc tgcacggcgg gatgaccggc aagcacatcg
     3361 gctggggcat ccgccagccc ttcgcgaccg gcaaacgcct gaagttcgag ccggtcaagc
     3421 gtgatcagcg cggtgagata tttgcgccgg tcgccgcaca ccagaacatg gtccacaagc
     3481 ggcatcatcc ggatcagacc ttcgatgttc tgcggggcga tgtttttgcc gcccgccgtg
     3541 acgatcaggt ctttcttgcg gtcggtaatg cgaatgaagc cttcaccgtc gatttcggca
     3601 atgtcgcccg tatgaaacca gccttcggcg tcaatggccc gggcggtggc ctccgggtcc
     3661 ttgtaatagc cctgaaacaa gcctttcccc cgcaggagca gttcgccgtc cggggccagt
     3721 tttccctcca tgccgggaat cagctggcct acggttccga atttgaaaca ctccgcgcga
     3781 ttcaccgcca cgggcgcaac ggtttccgta agcccgtaac cttccagaat caaaagcccc
     3841 acggcatgaa aaaactccaa aaggtaaagc ggcatgggcg cgccaccgca catcagatac
     3901 ttgacccggc cgccgaaaat ggcgcggatt ttccgaaaca ccagacggtc ggcaagacgc
     3961 agccggatag ccggcccgaa aggcaggggg cgcttttcgc gttgcagccg ggccgcctgc
     4021 atcccgacat ccaccgccca gcggaaaatt cggcgcttcg cgggggaaga cgcttgcgtc
     4081 atctgatgaa tgcggttgta aatccgttca aaaatctgcg ggacgctgac ttgcaccgtg
     4141 gggtgaagct cctgaaaatt gtcgatcagc ttatcgatgc tctccgcgta agcgccgaca
     4201 atgcccagat gcagggccgc atagacggtc atgcgctgaa gcatgtgtgt aaggggcaga
     4261 aaggcgatac cgaaatcgtt ttcttcataa tgatgcatct ttgtggcgtg aatggtcgtg
     4321 taatagagat tggcctgcgt gatcatcgcg cccttgggcg gtccggtggt gccggacgtg
     4381 taggcgatcg tcgccaggtc ggaaggtttg acgccgcgcc agatggcttc aaagcgcgac
     4441 ggatcggacc agtgcaggca ttcacctcgc tccagataag cgtcccgtgt gatcacaccg
     4501 tcaggaagct tgtcgcgggc ggtttcaaaa agaacgattt tttccggaag ccccgtttcg
     4561 gcctgaacct taagaagcct gtcgagcatc gcgtcgtctt ccgcaaaaca aatccgcaaa
     4621 ccgctgtgcg aaacgatatg cgtcatctcc cagaggctgg cggacggata cagggcaacg
     4681 gtgacgccgc cggcggataa aacggccagg tcgatgccgc tccattcgcg ccgggtgcgg
     4741 ctggcaatgc ccacggcgtc gcctttttga aaccccagcg cgaaaagccc catcacccct
     4801 ttcggcagca aatcccgggc ctgccgccag gtgatggcgt cccagcggcc cgctcttttt
     4861 acaagcgaca tcgttttatc gccaaagcgg cgggcctgat gcataaagat ccccgtcacc
     4921 gtcgggggat cgtacatctc cggatatgga tgttctgaaa cgctcatgat ctgccttgaa
     4981 tcatttaact cttttttttt gcttaccgga cactaaaaac ggtttattat caacagtctt
     5041 ataatagcca cattcgtcat tcccgcgaag gcgggaatcc aggcttattg aactggaccc
     5101 cggccttcgc cggggtgacg accctgtggt cttcccggtc ttgccgtgcc accctctggt
     5161 gtggtgacct tcaggttccg aaagccggga accagtttga taattcctgg aagccggcct
     5221 tcgctgcata accaaatgaa aattcaatta taagaccgct aatcaaagct ccggtccgcc
     5281 ggtggcgggc ccgcccggcg cggtggcgtc gtccatgttc cttccaaacc gtaggcggaa
     5341 tctcctccgg acgcctggct ccagttgcac cggttgcagt aaacatcggc ggcaacggcc
     5401 gtacggctcc acacgctccc cgttttgatc cagccgcgcg tcaccagcct ggttgcagtt
     5461 tccacttcca ccgcgtcggc gctgatgaca atgggagact ctcccggacc cgacgtcaga
     5521 ataacgtcac ccgctcccct aaagaccacc tttccaccgc tgccgaggcc gccaaacagc
     5581 ttgacgagcg tgttggcgtc aaggttcgct ccagcggaaa tcgaaagcgt gccgtaatcg
     5641 cctggcgcgc ccgcccgaac gacatccgcg ctcaccgcgg cgcggcccag aatggttacg
     5701 cttccgtttg cgcccgaatt ttcaatatga acagcgccct tatccgccac aacattaaat
     5761 ccgtagcccg gaccgtcgat gacaatgccg ctgcccggcg aggtgatccg gacggcggat
     5821 aaagacgcat ccgtctgggc ggtctgcaag agggcaagca cctgtcccga cccggcaatg
     5881 gaaatgccgg tttcggatgg ctcatcattt gcggacgcaa gcgatatttg ccccggcacg
     5941 ccctgagacc acgacgacca aggcgcggcc tgcgtctgca cacgcccgcc gacctcgatc
     6001 gtgccgcacg caagcgacag ggccacatcc cccgcccggt tccggtctgt ccatacctca
     6061 accagcccgc cttcgtccac ggagcaatgg ccgccggccg atgaaaacac ctgatccgcc
     6121 ccccagatcg ttccggaaag gcggatatca cggggcgcgg caagactcac gcgggcaaaa
     6181 ccgtcaatgc gccgcccggc gggaatcgtg acgtcgccgg caaaggccgt cgccgaaaaa
     6241 gcgctgtccg atcgcttttt ttccgtgcgc aaagccgttg attccacccg cagagcttcg
     6301 gctcgcgcct gctctggcgg tgttcgcaat gccacaaagg ccgtgtagtc ggcaaacgcc
     6361 agggcggcta attcgtccgc gccggccgtc cggccgtaaa gggccgtata gcgcgcaagc
     6421 gtcgacgtgc ggctggcctc ggtcgaatcg accgcgtcga caatccgcac gtagcccaga
     6481 cggatcaaat cctgccggat aaacggcgcg aaatactggg gcgcgccgcc cgctcccgcc
     6541 gtatccatct gcggattatt gcggcagacc gagtaggcga caggcagcgg ccggaccacc
     6601 tcgtaaacag cgccgaaccg gcggtaggtg aaatgccccg tcgcgccttc ggcccgccag
     6661 ggccccactt ccacccgtcc gttgaaaaac gcggcatcca gccaatcgcc cgacatcgca
     6721 tcgcgcagcg ttgcttccga cgtaaaataa ttggaatacg ccaccgtgga gcggtcgctg
     6781 aagtccagcg aaaacagatt gctcgtcgcg ccgttggcca gtcttgcgcc cgcggggatc
     6841 ggatcggaat ttccccattc atccacgccc gcgtactcgc cttgcccctg cgacatgacc
     6901 gcccgctcgc gataccccgg cgtggcgacg gacgccacat acgtgctttc cagcgtcacg
     6961 gaggggccgt cgagcgtcac cgacccgtcg cccggctctc cggaaccgta acatccccat
     7021 agtaccgaaa ggaaaatcag taaagcgtaa aacgtccatc gcgggttggc ggcgttgcgt
     7081 gcttccataa taaaaaaatc tccttttcca tcttctcatt gaaaaacgaa ttaaatcttg
     7141 ttatgtaaaa tcactctaat tcctacacca cacgatttca accacggagg aactgcttat
     7201 ggcacagaac atgaatctgg tttcatccgc atccccagac gacccgtcac cccgcccggc
     7261 cccgcccgca gccgacgcgt ttcccgaaac gacgcgcatc cagcgcatcc ggcttctgaa
     7321 acaaatcggc ctgtatccct tttttgttcc cagcagcgga caggtcggcc cgcgcatgct
     7381 gatggacggc catgacacga tcatgctggg gtccaacaat tacatggggc tggccaatca
     7441 tccggaaatg attcaggccg ccgtcgaagc gacgcggaaa tacggaacca gttgcaccgg
     7501 ctcgcgtttt ctcaacggaa cgctcgacat ccacaacgag cttgaagaaa agctttcggc
     7561 gtttttcggc aaggacgcgg ccatctgctt cagcacgggc atgcaggcca atctgggcgt
     7621 catttccggc atcacggaca aggacgatca tatcgtctcg gatgagaaaa accacgccag
     7681 cattattgac ggctgccgtc tgtcctacgc caaaacgcat gtctacaagc atgacgacat
     7741 ggaaagcctt cgtcgcacac tgcgcgccct gcccaaaaac ggactgcgct ggatcgtcag
     7801 cgacggcgtc ttttccatgg aaggctccgt cgccaatatt ccggaaattg tggcgctggc
     7861 gaaagaattc aacgcgcgca tcatcattga cgacgcgcac ggcatcggct acatgggcga
     7921 tcacggtgaa ggctgctgcg gccatttcaa tctcatggat cacattgaca tcctgaccgg
     7981 aactttttcc aaatcgtttg cctccctggg cggcttttgc gcgggccgcg cggacctcat
     8041 ggaccattta aaacaccacg cccgcagtct gatcttcagc gcttcgatgc cgccggccat
     8101 ggtcgcctgc gccatcacgg ccctcgatct tttccagcgc gaaaagcagc tgcgcaagca
     8161 agtcatggtc aacgcctcct acctgcgcat cggtgtgcaa acgctcggct tccggactgt
     8221 cgcgcattcc attcccacgg ccatcgtccc cattatggtg ggcgagaacc tggcgacgta
     8281 caaactgcac aaaacgctgc ttgcctgcgg cgtgtatacg aatccggtcg tgccgcccgc
     8341 atccgccgaa gggctgctca ggctctccgt catggccacg cacaccgaag acgacctgac
     8401 ggacgcgctc aacattctgc accgggtcgg acgcgaagcg gggctgatct gaccggctga
     8461 atttaatagg tcagaatcag cctggccccc agctgataat aattgtacgc accggacgcg
     8521 ccgacggcgg aattgccgcc ttccgccgag gcctccagcg ccgcgtgttt tgaaaaccgg
     8581 tatcccagaa ggcccgcgcc gccgataatc atgttgtagt tcccttcttc attgcgtgta
     8641 aacgactgat acccgccgta gggctcggca tagccgtaaa agcgcccgcg ctcaaacgat
     8701 gcgtttacga aaaggacatg agcgataaag ttttgcggat cgaaatagcc gtgcccgctc
     8761 tgtctgcgaa aattcatgta ccggatgcgg tacccggcgg cgacggctgc gggcctgcgc
     8821 cagatcagac ccccggcctg gcccatgacg tcgtgggcgc tgttgtcgtc ggaataatcc
     8881 cggtaatgat agcctgccaa aagcgtgatc cggtccgtgg cgcgatagac gccggacagg
     8941 cccgcggtaa gcgctttgac atgatgctcg atgacgcccg ccgtgtccga aacgctgtcc
     9001 acacccaccc gcgcgccgac gctgccgttg ggaatatcca cgtcgccgcg caggctccag
     9061 gcgacgcccc ggcccgaatc cataagcccg acgccgccgc ccacgccccc gtaaaagggc
     9121 attcgggaat acgtcgacac aaacagacga tccgacgagc ctgtggtgga tgcatcgcgg
     9181 ccatccgtcc gggtataatc cacggtggtc ttccagtttt tcagccacac cgatccggcg
     9241 gcaaaaaagc gccacacctg gttttggtcg ctgtcgctgt aaaacgtgaa ccccggcgcg
     9301 acggagggat tgaacgtgat gtccctcaat tctttcaact cgcccaggct cttttcttca
     9361 taggcaaacg cgggcttcaa aagaggcagg ctctgatccg cagccacgcg atcaccgctt
     9421 tgcagataac tgtaagtcag cccttcgcgc gcatcatagt catcttgctt caagaccagc
     9481 tgcgaataca gagcgacggc cggtcggtag ttgcgcgtca tccgcagtcc gcttgcccgg
     9541 accagcaggg cctcccggtg ggacggctcc aaggccagca cccggtcgat ttccctgagc
     9601 gcggcgtcat attcgccgga ccacaacagc acgcgcgcca ggtgaatccg cgcggcgctc
     9661 atctccggat ggtccgttag gatttttgtg agccgctcgc gagccgcggc aagattgccg
     9721 gcccaggaca tgacgcttgc catccgaagc tgttcgtcgg cggtaaacga tcccgaaagg
     9781 gacagggctt tggcgtattc ctcggaggcg tcctgatgcc gccccgcttc ggccagacgg
     9841 tttccctccc gggcggcgag gcgcgccagt tcatcgggcg acggcgtccg gggtttttcc
     9901 gcctcggcaa gcgccagctg aagcgtcgcc gcttcctggc gctgataggg cagctcgggg
     9961 ctgagcttct cggcgctctg gcgggccagg acgaccttgc cctgggccag ataggcgtaa
    10021 gccagaccga ctctggcctc gaaatgctct ccggccgtaa gcacgctttg gtaaacatcc
    10081 acggcttttt cctgatcgcc ttgcacccgg gcgacatcag cggaaagaag cgtcgcgtcg
    10141 cgatccgccg gcgcaagctt caacgcctct tgagcgtccc ggctcgcctg gtccatctcg
    10201 cccatccaga aaaacacgcg gccgcgctga acgcgggctt tggcgttttg cggatttttc
    10261 tccaaaagtg ccgtaagctc acggcgcgac cggccgagtt ttccgcccca ggccatcacc
    10321 gtagccatgt ccaggcgttc gtcatcgcca aaatcaaccg ggccggacag cgcatcttca
    10381 tacgcctggg cggcgcgcgt aaagtcgccc tcgcgcgcgt agcgatcgcc ttgttccttg
    10441 atcgtctggg cgtcatcggc ccggacggcc ccagccgccc agagcatgca cgccagcgcc
    10501 aaaagcacac cggccgcccg gatcagggcc cggtggccct gtcgataaga tgtcttcggg
    10561 gctttcatca gctcacctca aaaattgatg gaaaggttaa aaggttgtcg tcgggggtta
    10621 tattgttgcg cggaatgtcc cagggggcgt ccgtccgaag gtaaacgccc cgcttcgacg
    10681 tcagcagata ccgaaacccc gccttcctgg cggccgcgac gctcttttcg gtgtaaaagc
    10741 cgtagggcca ggccaggatg tcgcagcgct tccccatctc ccggacaaag tttttctgaa
    10801 attgcgcaag gtcctccaga agaacgctgt cggcgacgcc cgccgcgccc tgatggcggt
    10861 acacatgcaa acgctccgta tggcagccga aatcgacaag gccggttgcg gccagatagc
    10921 ggtattcgtc ccaggcgagc atcgggcgaa tgatcccgtc tttgatgtgc gaagcgacat
    10981 gctgcccgat aatgttgatg gtagccttga atccatacgc ctgcaaaaaa ggcagggcgt
    11041 agggaataaa tgaggcgtag ccgtcgtcaa aggtgatgat gcaggcacgc tccggaagcg
    11101 cggatgacgc cagatcggca aacgagacgg cgcgataacc ttcggcgcaa agccattcca
    11161 tctgggcggc gaaaagggcg ggcggcagcg tatagtcgtc ggtgtgctcc ggggagatgt
    11221 cgtgatacag caaaaccggg atggcaacgg ttttcgcggc ggcaaatgcg gcgcgaggcg
    11281 tttcgggtaa aatcagaacg gccgccagaa actgcaaaaa agcgcgtctg gtcaaatcct
    11341 gcgtcatggg acaatctcct taagatagcg ccggtacacg gcgaggctct ttttttcata
    11401 aaaatgaaac aggggcacat tccacgtcgg ccaggcgtaa ggccggatca aacgcggctc
    11461 catgacaccg ggcagccggt ccgtcgcgtt gtccgacttg cccgtgattt cagccgtcca
    11521 gccgcgtccc gacggcttga aggaatacgc cacaaacctc agtccctccg tctgccgggt
    11581 ccatttttcg ccttgtttcc agtagacggc ccgcgaactc ggatccgtca ccatcaaaag
    11641 cccccaggcc aggcgcagtt cgatgagatt gcccgacaca tggaaatcgg ccagcgaatc
    11701 atagaggcga tctccgggga taagcgagcc gtggcgcagg gcgcttagcg gaaacacgcg
    11761 ggccggatag acgcggtcgc ggtttttgct gatgcgccgg tcgttggtct tgtgatgcat
    11821 gctcacccac gccccctccc ttgaagaccg tggccaaagc tcctgatacg gatcgttgag
    11881 atatttgtcg tagcttgcgc aaatcagcat acggctggtt tgcacgcccg acagatgcac
    11941 caggaatttc agcccgacgg ggctttgcgc gttgaggcga aacggcagcc gaaattcgcc
    12001 tgtggccgga tcgcaggtat cgatgccgat tgcgtaatgc ccccggttga aatcgacggg
    12061 gtcgcccaga gtcagcgcca gatacaaaaa tccttcgtcg tgctggaccg caagcgagcg
    12121 caggcgtcgc gttccgtcgg cgccgtcacc gaagcagtgc gcaggctgat cgtccttgcg
    12181 gtacagcacc gtggcatctt gccactgggc cgcatcgcct gaaagcgtga ccttctttcc
    12241 cggatagccg ggatacgcgc ccaccaagcc gtagcactgc tcggcgtcct ggaggttgaa
    12301 ccaaagcgca ttgcgctcgg cggggcgctc gtaatttaca aaaagccagt tgcgtttgaa
    12361 ccattcgtca aaccaggaaa acagcgcgcc gcccgccagg cccgcctccg caatggcctt
    12421 gagcatctca ccgttgatcc ggccctgcgc gtcttcatca tgcccgccgt gatgccatcc
    12481 ctggcgctgc cagtgcgaca cctcgcggct agacggcacg ccgtattcgg caatcaaaat
    12541 gggctgggtc ttgtggtgcg cctttaactg gctcaaataa gcgctgtagg gtttttcccg
    12601 gtccaggtag tcgttgttca tgaagtccgg atagtagggg taggcgtgat acgtcgcaaa
    12661 aaagccgctc cccttccggg cggcgatctt tgccgtgtcc agggtttcgg cgtcttcgtt
    12721 ttcgttgcac tccgaggctt tcacggccag tccctgccag cgcaggccgt cttcatacag
    12781 ggactccgac ggatgcgcca ggggatcaag cgtcggccag cacacggtcg agacgggatg
    12841 ggttacgccg tattttcttt cttcgtaaac cagaagctcg tcgagtatcc ggcagatcca
    12901 gcgttcaaaa ggcgaaaggc gcgcccccga aaggaaagcg ccgttaaaga cactcccctc
    12961 agcgcttctg aggcggttgt atttgccgat ggggcacccc tcccactccc ggccgaagat
    13021 gatgcctgca acctgtggcg agacatcgcg cgtgtagacg ccgtggggct ggccttgcct
    13081 ctccggaagc gtgatccgtc caaacagggc gtctatggcc tcggtgatct ggcggcgggt
    13141 gtagcccaga aacgcctccg aggaaaaatc gccgtcgtcg ggcggttcga tccagatgcc
    13201 ttgaatcaga taaagggcgt tttgtccgtc atttgcctcc gccagcgctt cataaaaaga
    13261 cggcgactga atggcgtaaa cccggaccgt attgaatccg gcatttgcca tctgcccaaa
    13321 ccagcgccgg tactgcgttt tgccgatggg atactccccc ggatacgtgc cgggcgccgc
    13381 aagccccaga ttaatacctt tgacaaaaaa cggccgccag accccgccaa cgcgtttttc
    13441 aaaccccgcc ttgcccaccc ggaaacgcac atccggctgg gacggatgca cgggacgggg
    13501 ggccttcata cgttcttcaa tgcgccgcgc cagcttcagg atgtcctgat ggccgggcga
    13561 atacgcaagg cccttgtgcg cggcccagcg ggccgcgggc gtctcgtcga gccccagaaa
    13621 ggcccgcgcc aggcccatcc agccctcgac gctttcatcg tcgtcgagcg cgcggcggaa
    13681 gatgttgaga gcctcctgat aatcgccttt ttccagagcc aggcgtccca gtttttcaag
    13741 ctgcatgggg tttggtctcc ttttgacgat ggtattccca gcgccttttg ccgaagagat
    13801 actggaaaaa cgcctgaaag cgccagaatg agttgatctg ccggtagccg aagttctcca
    13861 gaacgccgaa caccagcagg ataaacagat gctcccagtc ggggtagcgg tgataggtga
    13921 tttcttcgag aaagatgctc acgacggaca aaaacacgcc gtaaaaaatc gccaggaaaa
    13981 taaacaggaa gaacaaattg aaattgatca gattaaagac gtaagccccg accatggcca
    14041 catagcccag cacttcaata aaggggctca gcatttccgc gaaaaagtaa tacggcatcg
    14101 tcagcatgcc caccttgccg tagcggggat taaacaccat gctcttgtat ttcatcatgg
    14161 tctggatgag ccccagatgc cagcgcctgc gctgcccggc cagttgtttc atcgtctccg
    14221 gcacttccgt ccagcagatc gggtcggtaa taaagcgcac ggcatacggc tttttggcca
    14281 gaaggtgctg tttgtggatg cggacgataa tttccatgtc ttcgcagacg ttgttgattg
    14341 caaagccgcc cgcatcaatc accgcctgtt tttgaaacat ggaaaaggcg ccggacaaaa
    14401 tgagcgttcc ccccaggcgg tcgagccccg cgcggccgaa caaaaagccg cgcagatatt
    14461 cgacgatctg gaagatgccc agccatcggg acggcaggcc gattttcgtg atccggcccc
    14521 cgtcaatcgt cacgccgttg atgacccgca ccacgccgcc gcaggcgacg accggcgtcg
    14581 ggctttccat cacggcggtc atcagccgca aaagggcgtc gggctccatg agggtgtcgg
    14641 cgtcaaccga acagacataa gggctgcggg aggcgttgat gccgcagttg agcgcgtcgg
    14701 atttcccccc ctgctccttg tcgacaacca gaagattggg gatgtttcga ttgtagtaaa
    14761 aaccccggat cggtttggtt ttgaggaggc tccggtaaac gagatccacc tgttttagat
    14821 cgtattggcc gatcagttgc aaaagcgtat cgtcgcggga cccgtcgttt acgacaatca
    14881 cttcgcagtt gggatagttc atatccagaa tggactggat ggtccgaaag aggatgcgcc
    14941 cttcattgta agccggaatg atgaccgaca ccggaggcac ttcgggagag aaccggtttt
    15001 ccagaagcgg caggtacttg atcttacgga tatgaattag gatggagccc accgcaacga
    15061 aaatcagaag ggtgtagatc aggttgacaa acccgtaaaa gaagccgatg aaataattga
    15121 aaccgacaat ggcggcaatc agcatcgctc tcatcttgcg gctccggtca gaaaggattg
    15181 aatgtttctc atgcgctcgg ggtcatccgt atcgaccagg tattgcttaa gcggcgaaac
    15241 aaaaccacaa gacaccatca tggtcaggat ttcatgcgct ttggcggatt ccccttcccg
    15301 gccgcttccc agcaggtcca gcaatatctc gacaaagccg cttttttcca tctcttcgca
    15361 gacgctctcc cgggcgtagc ggtcgtcgga catcagcagt gtccggaagg catcgacggc
    15421 gtccctgccc agaagcgtca gcgccagcgc ggcctcgttg cggacctgcc agcgttcatc
    15481 gcgcatcagc accgcaatgg cggcggcgtt tttcgcgtcc cttcggcgac caagcgtccg
    15541 caccgcctgc agacggacat accacaccgg gtccgaaagg agacgtaccg gcgcgtcgtc
    15601 atgcagggtg ccgaattttt cctcgcaagg cgcgatcagc ttcagcgcct tggcgcgcac
    15661 ttccgcatca tcgttcccca ggtgagacac ggcaaattca taaatctcgc gggtggcggg
    15721 aaagtcgtac atcacctcca gaagcgacgc ggtcagccgc gcgtccgtgc aggtcctggc
    15781 gtaagtaaga agcgtcggca aaatgcgggg ccccgccgcc accagcgacg catcaatggt
    15841 tttctgggta acgaggttct tctgcaaaag accgggaagg cgctccagaa gcgctaacag
    15901 ctcggggcct tcgccgattt tgccaagcgc gcgaacggcg acggaggcaa tgtcggcatg
    15961 atccgtctga agcatctcca ggacgggttt tgccgcgcgg ggattggcca tgcggccgag
    16021 cttggaaacc gcctgagccc gggcaatgac cttgcggccc ttcaaaacgc caatgtagta
    16081 atccacgtag cccaggcggt caaacaaccg cgcggccgtg gtgcgggtcg cctccgcgcc
    16141 cattaaatca aaaagattgt attccagggc gacccactca gtggactcgg gcgtcgccgc
    16201 gaagcgctta tgatactgct caagcgaggc cttgttctgc atgtaatgct tgagttcaaa
    16261 catctggcgg ctgcgaacat tgtccagaac cgcgtagcgg tgcgcattgt acagacgtct
    16321 caacaccgcc agaaggaaga agagcaggca aaaaccgatg atgatgaaaa tcaggacgag
    16381 caaaacgatt tccatccgtt ccatgccctt gaacatgtca aggaccgaac cggacaggga
    16441 agacgcgttt tcattttgct tggcgggcgg gcgcgcggag ccgtccgcgg cgaaggcatt
    16501 taccccaccc gaaccgatgt ttaacgtggc atccgcaatc tctgtggtta cggccatcgg
    16561 gattctcccg caacgatcaa aacatttcca gacgataatc ttctgacaaa cacatggcgg
    16621 cgacggtaca tgccgcttga taaggtatcg acattttcag gaaattactg aaattggcgc
    16681 ttttttccat tcttgcaaac cttgtttggt tggcgcatgc cttcgtgtta taatgccgcc
    16741 cgcacaacag gaggaaaaag atgagcggga aaaatagcag cggcatgatg ctggcggtga
    16801 aagtcagtct ggcgagcaat gtcttgctct ttatcatcaa ggcgatcacc cttttcattg
    16861 tggattcact ggccgtggcg gcggatctgg gcatttccgc catcgccctt tgcgtctccg
    16921 gcattctgta ttacgccgtc aagatgtcgg acaagccatt gataagaact tcctttttgt
    16981 gggccgaagt atattaagcc gtctcttgtg tgtcaagaag tttttgccac aagatgttgg
    17041 gtgtttcgct gatgatcccg ctgaaggtat ttggcaacat ttgttctatt gagaaatcta
    17101 cacacctttt gatctattcc gatgccagga tggccaattt aaccgaaatt cagacaattg
    17161 ataagaactt cctttttgtg ggccgaagta tattaagccg tctcttgtgt
//
LOCUS       DAOSIM010000018        17035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_3560_length_17035_cov_8.959600, whole genome shotgun sequence.
ACCESSION   DAOSIM010000018 DAOSIM010000000
VERSION     DAOSIM010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 17035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17035
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3560_length_17035_cov_8.959600"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PLR47_01740"
     CDS             complement(<1..267)
                     /locus_tag="PLR47_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOG11686.1"
                     /translation="METDEDSKMSLTEHLVELRKRLTNVLIAVGIGFLACYSFKDYLF
                     AVITKPLTEALPKSSYLIYTGLTQAFFTYMKIAIFSSLVLTSPFI"
     gene            complement(316..594)
                     /locus_tag="PLR47_01745"
     CDS             complement(316..594)
                     /locus_tag="PLR47_01745"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOG11687.1"
                     /translation="MFGIGVQELIIIAIIALLIVGPKKLPDLAKTLGKGFRDFKNATE
                     GVTENLKDALKEEDKPKSDDHLKDSLPFDKTDAEEAKNEAPEDDAGKT"
     gene            764..1279
                     /locus_tag="PLR47_01750"
     CDS             764..1279
                     /locus_tag="PLR47_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11688.1"
                     /translation="MKLEEQIAELTRAGSGTLWEPARLAAVVLAASAALAGAVTRNPF
                     FLGIALALSILAFAVRQTAPHIANAVRGLREGVKQPGTVEISLHQWTDAESNVHESFH
                     GVISMDGQPAWEMEFVTPQNWQPREGGFQAELAFIRGVEWPVAVLTREGLLYPRRKPL
                     RAGRPPSSQSG"
     gene            1360..1851
                     /locus_tag="PLR47_01755"
     CDS             1360..1851
                     /locus_tag="PLR47_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOG11689.1"
                     /translation="MPIYEFYCRKCNTIYNFFSRSVNTDKIPSCPRCKNVKLSRRVSL
                     FSTITGRAKDGEEPDPFRGMDESKMEKAMMQLAAEAENIREDDPRAAADLMRKLSDAA
                     GMKLGDSFQEALRRLEAGEDPDKIDEEMGELLSAEDPFGEKKKAGKSAVRKPRLDETL
                     YDL"
     gene            1879..3129
                     /locus_tag="PLR47_01760"
     CDS             1879..3129
                     /locus_tag="PLR47_01760"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOG11690.1"
                     /translation="MRVLTFRVTLVVLFFLCCGWASAAVRISQLPPPPPTAKLRVFVV
                     AVTTEFKSAKGPVFWPVSPEEFDRIQKKAIGERLRQQGIYEVVADSDLRAALGTQKPA
                     GWEWLADDCRLVREVGRALHADYALVSERSFAIHLQFDTRLMNLHSGKEFAAEGYVPS
                     TMLRAMNKEQKTLAGGEAVKIQFRQIFQEARGDLMRTAVRKGKIATKGSGRDEKTVPP
                     PLEEKAAAGSQPLEKGLQAAQASKDKAPAATLQKTPPPPAAVEKQRAFEKELERALNP
                     GDQKKAGPRLIVYDFEASEPMKVAGLILTEALREALHHSGNFVLIQRENMVKFLEEYK
                     LQQSGLVDKKQAVRAAKWLAASEAVTGNLAVLGNTSLLQAKRMDIETLGTLAAGSIKC
                     PAGREDELLERMPELAAMLTGGKK"
     gene            complement(3150..3431)
                     /locus_tag="PLR47_01765"
     CDS             complement(3150..3431)
                     /locus_tag="PLR47_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11691.1"
                     /translation="MKCCAHCQKEIPEGEPAGRQAQCPFCGADLHICLNCVFYEPGIY
                     NDCRESQAERVVEKSRSNFCDYFQYRTGAAKPGRRPADLQEKLGALFKK"
     gene            3563..4039
                     /locus_tag="PLR47_01770"
     CDS             3563..4039
                     /locus_tag="PLR47_01770"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HOG11692.1"
                     /translation="MADRSKLGMEFPVYSIRVEKNKIAEFVAALEQKEDTEQIHPHYR
                     DADAAKKAGYANIPTPPTFPTSHVFWTGGGLLGTIKAVGADLTKLLHTKEEYEYFAPV
                     CAGDVITRSMKVVEMYERGKKEQKSRYMQVTVLQTDMINQHGVLVIRARTTFMERG"
     gene            4045..4494
                     /locus_tag="PLR47_01775"
     CDS             4045..4494
                     /locus_tag="PLR47_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOG11693.1"
                     /translation="MALVFDGIQIGDIMPPYTSAPITRTHLVRYAGASGDFNPLHHDE
                     TFAKAVGLETVITHGMFIMGIAGQAITSWIDNRWLRKFSVHFLSMTQPVDLNDYSNTK
                     DRATITVTGKVVDKYEENGETRIRCELTARDALGSRKLKGYFVAALP"
     gene            4529..5857
                     /locus_tag="PLR47_01780"
     CDS             4529..5857
                     /locus_tag="PLR47_01780"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG11694.1"
                     /translation="MKARIVVIDDDAVACEFLQEALQREGYDVTAFTSAPAALTLDLS
                     DYDLLMSDIRMPGMDGLQCLSEIQKRWPNLPVILMTAYGSLETTMEAIRLGAWDYISK
                     PFSPEDCRALVKKVLEVRELRKKRLIEPAEDADETKLIGSSPVMVEFYKQIARVADSQ
                     ASVLIEGESGTGKELTARSLHSMSSRRQKPFVVVHCGAIPDNLLESELFGYEKGSFTG
                     ADHQHVGLIESASAGTIFLDEITEMSTALQSKLLRFMQDGEVRRIGGHDVRHVAVRVV
                     AAANKNVDEEVAAGRFRLDLLYRFIVRLRIPPLRERRPDIPLLIETMLKKLGYPDVRV
                     SPEAMETLMAYDWPGNVRELENVLRQTLLLSPFVVILPENLPDRFRTRAAVKAQADGP
                     QWTPLEEAEREQIVQTLKKTDWNQSRAAEILGIDRKTLRTKIRRYGLQEK"
     gene            complement(5861..7408)
                     /locus_tag="PLR47_01785"
     CDS             complement(5861..7408)
                     /locus_tag="PLR47_01785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG11695.1"
                     /translation="MLKRLLSKIIDYNNHLTLRVRLVALTVAGLALTMAVWGLIHLTA
                     LDRILVDQQGKRLEGVAETVSTFYQHFPTRQGIATLDSTLKDHIQTDVRLARIDILEI
                     RRGSVNYVAGASRVAYEWPDHLVAETGAKGKSLSMKLETEDGPSLGLLYPVPGDSKDS
                     RVVVVGIIAFSRANAEIMSRARRLLLVSSAGLLLAILLLLGLSYGWLIGRPLETIINT
                     IDEFQKGRYVERIPAGRRDEWGLLADHFNRMADEIENVMERNDNLTRHLADRVREETL
                     NVIGLQKQVDDLKQLTALGHLTANLAHDLGTPLHSIAGLARLLLEREGWPPDVAHKLN
                     VIVEQTQRLDHVIQNVRKATRLPEPHLERMTVHHILSETLPLVEPLIQKNRIAIEVRL
                     ERSAASLYADRYRIQTALLNILQNSIEAMSGGGVIRLSAEDDRAARTIAISIADNGPG
                     IAADLLKRVCDPFVSSRQGEGMRGLGLAIVRDIIRAHNGRMDIQSTPESGTTVVLYLR
                     MADAAAD"
     gene            complement(7563..8063)
                     /locus_tag="PLR47_01790"
     CDS             complement(7563..8063)
                     /locus_tag="PLR47_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11696.1"
                     /translation="MKRLALLVVAVVFAFATMAMAADKPAVSTVEEVQSGATKISDAD
                     KAAVKDAQKAAKEKRAAKKAGLVKKEKVKAPEAKKGVSAVEEVQSGATKISDADKAAV
                     KDAQKAAKEKRAAKKAGLVKKEKVKAPEAKKGVSTVEEVQSGATKISDEDKALIEAQR
                     KAKKAK"
     gene            complement(8146..8526)
                     /locus_tag="PLR47_01795"
     CDS             complement(8146..8526)
                     /locus_tag="PLR47_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11697.1"
                     /translation="MKKVLVCAIGLVFLLAVFAFAAEKPGAHSAQKVVSKTATMNARG
                     KVVEISDKAVRIERSIKGSAEIMEFALENPAPGIAVNDFVKIDYTIKDGKMTASRIAQ
                     PKAEKNHRKNETKQVKEKSAATAK"
     gene            complement(8711..9766)
                     /locus_tag="PLR47_01800"
     CDS             complement(8711..9766)
                     /locus_tag="PLR47_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraprenyl-beta-curcumene synthase family
                     protein"
                     /protein_id="HOG11698.1"
                     /translation="MQTPTRILSLTARMILQVRPAARTFLQGWKERARRIPDPELRRQ
                     ALASIEAKTFHCEGGSLCGLTAGHFCREAIEFIVAYQTISDYLDNLCDRSTSQDPEDF
                     RALHEAMLHALTPEAPLAAYYRFRPERNDGGYLDELVRTCQMILGGLPEYHRVAPFIR
                     ELAGLYVDLQVHKHVRPDEREERLKKWFDGLRPGLPEMAWYEFAACAGSTLGIFCMVS
                     LLFGKQDAAPAVERIRRAYFPWVQGLHILLDYLIDQEEDRREGDLNFCSYYENHERLT
                     QRLGHFYRRAQAGVADLPDAKFHHLLISGLLSLYLSDHKVSGQKTVRAVAKKLLALGG
                     AETFFFYLQCRMYRRLP"
     gene            9943..11289
                     /locus_tag="PLR47_01805"
     CDS             9943..11289
                     /locus_tag="PLR47_01805"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG11699.1"
                     /translation="MDRTAMEADVVVVGGGASGLMAAGRAAERGARVLLVEKMEGCGK
                     KILVSGKTRCNLTNAADLKSFVSMYGPNGRFLHGAFSRFFRPELLAFFERYGLVTKAE
                     RGGRIFPVSDDARDVLQVFHQYLEEHHVRRQLHARVTSVALRPDSLFVVETTGGSCLS
                     SSVIFATGGASWPATGSEGDGYALSAALGHTIVKPRPALVPLVVREQALARSMQGVSL
                     RNVRATAFQGEAMTIDDALTPTYVSGRGASKTPRPPVIESRFGEMLFTHFGLGGPIIL
                     LLSLAVVDALEQGPVSILIDLKPAVTREQLGKRLQKDFNAFGKRKTATIWKDYLPAKM
                     IGPLAALSGVDENKPAHQVGAAERGKMIDVLKALRFNLQAPLPLAKAVVTAGGVSLRE
                     IDPRTLASKKVPGLFFCGEVMDIDADTGGYNLQAAFSTGYVAGESAAAFAAAKKRP"
     gene            complement(11374..13740)
                     /locus_tag="PLR47_01810"
     CDS             complement(11374..13740)
                     /locus_tag="PLR47_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG11700.1"
                     /translation="MSKPTAKKASPKKTRGSDSKENFFDRTIGNDEKRYRLLAENLTD
                     VIWVLDLQLKHTYVSPSVERLRGYPPEEAVRQSLEEFLTPDSHKKVLDIFSREFSQET
                     QGQRHAREWTQSIEVEMLRKDGSTIWTEVKASLLYDDHGRPEGILGITRDISERKKTE
                     QALKKSEEYYRAIFEHTATANMILAEDTTVLSVNANFEKTMGYSKKEVENKMSWTRFV
                     VDEDLAVMVERHKTRRTNPESVPGTYEFRGPTRSGDIRTFFLSVEMIPGTTNSVASLF
                     DITDRKKAEDDLRQSEERFRDMARLLPETVFEADLNGTVTFVNEPSFDRFGFTRGDVE
                     KGMSLFNVLAPEDRERAGENFQRIIRGESLGLNEYIAQKKDGARFPVMVHTSRIFKEG
                     KPAGFRGFLIDISEKKAMEEQLLRAQKLEAIGTLAGGIAHDFNNLLMGILGNISLMLM
                     NMEQEHPFHERLKNMEEYVQKGSNLTRQLLGFARGGKYEVRTTHLGKFVARSAEVFGR
                     TRKEISIHHQAEKDLWYVDVDRGQMDQVLLNLFVNAWQAMPGGGHLDISVENTALENQ
                     DVAGFGVQPGRFVKLTVKDTGIGMDEATRLRIFEPFFSTKKRGRGTGLGLASVYGIIK
                     NHGGFISAESEKGTGSSFIIHLPASEKKADEEDQQESRPAQGRGTILIIDDEEMIVDV
                     GSQMLEGLGYSVLTAGGGRKGVDVFARNAEIIDLVILDMIMPDLSGRETFEALRSRNP
                     AVNVLLSSGYSLDGQAKELIAEGCRGFIQKPFTMAELSRKVREFIEKS"
     gene            complement(13830..15416)
                     /locus_tag="PLR47_01815"
     CDS             complement(13830..15416)
                     /locus_tag="PLR47_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOG11701.1"
                     /translation="MSKQLQKEIERRRTFGIISHPDAGKTTLTEKLLLFGGAIQMAGA
                     VKARKASKHALSDWMSIEKERGISVTSSVMKFEYADCEINLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDGAKGVETQTQKLMEVCRLRNTPIITFINKMDRDCLAPLDILADIEDK
                     LQIECSPLSWPIGMGKRFRGVYNLYDKKLHLFTPGKARRSEDGVVINDLSDPLLDELL
                     DSQARELREEIALLEGAANPFDIKEYLKAGQTPVFFGSAINNFGVKELLDAFVEIAPP
                     PVPRPTTTRIVDPDEDDFSGFVFKIQANMDPAHRDRIAFLRICSGKFQRGMHVMQHRI
                     GREVTLSNATIFMAQDRTNVEEAYPGDIIGIHNHGTIKVGDTFTQKEPLKFTGIPHFA
                     PEHFCRVRLKDPLRLKHLQKGLTQLSEEGAIQVFKPLWGSDNILGAVGSLQFDVTMSR
                     LKNEYSVYADYEKADYTAARWVTCDDAKKMEEFQKKNLMSLAMDTEGNLVYLALSDWR
                     LAHTMEEWPQIRFHKTMEKS"
     gene            15639..15713
                     /locus_tag="PLR47_01820"
     tRNA            15639..15713
                     /locus_tag="PLR47_01820"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15671..15673,aa:Gly,seq:ccc)
     gene            15867..16352
                     /gene="rimP"
                     /locus_tag="PLR47_01825"
     CDS             15867..16352
                     /gene="rimP"
                     /locus_tag="PLR47_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOG11702.1"
                     /translation="MYDELIDKIWRLAEPVILSEDMELIHVECVKMHSRWVFRFFLDK
                     EGGVTLDDCTAVSNQLGDILDVHDLSRGPYTLEVSSPGPDRPISRDRDFEKFRGAKVK
                     IRTHVKIEGVRNFQGTLMDYAEENGRKLVRIGMADRIFSIPRNDIARANLVDEDTVNA
                     L"
     gene            16415..>17035
                     /locus_tag="PLR47_01830"
     CDS             16415..>17035
                     /locus_tag="PLR47_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HOG11703.1"
                     /translation="MLHDLKRLIEQMGKDRGIDKQIITEALEAAMLTAARKKLGQDVE
                     IEAHYNDDAGEIEVFQFKTVVDKVLDPETQIAHEDARRTLDEGAEPGDSLGMKIETSS
                     FGRIAVQMAKQIIIQRVKDAERDNIYDEYKDRKGELINGFVQRFEGGNIIVNLGRAEG
                     VVPPMEQIHRETYKRGERIRSYLLEVKKNTKGAQIILSRTHPAFLKA"
BASE COUNT         3550 a         4742 c         4901 g         3842 t
ORIGIN      
        1 aatgaagggg ctggtcagca ccagggaaga aaaaatagcg atcttcatgt aggtaaagaa
       61 agcctgcgtt aagcccgtgt aaatcagata actgcttttg gggagagcct ccgtcagcgg
      121 cttggtgatg acggcaaaca ggtagtcttt gaaagaataa caggccagaa aaccgatgcc
      181 cacggcaatg agcacgttgg tcagtctttt gcgcagctcg accagatgtt cggtcaaaga
      241 cattttcgaa tcttcgtcgg tttccatcac gcgcctgtta tgccgggttt gaaatagcgg
      301 aaatggcgtt gaagttcagg ttttcccggc atcatcttcg ggggcttcgt ttttcgcctc
      361 ttccgcatcc gttttgtcaa acggcagaga gtccttcagg tgatcgtcgg atttgggttt
      421 gtcctcttcc ttcaaggcgt ctttcaggtt ctccgtaacg ccttctgtgg catttttgaa
      481 gtcccggaag cccttgccca gcgtcttggc cagatcgggc agctttttgg gcccgacgat
      541 caacagcgcg ataatcgcga tgataatcag ctcctgtact ccaataccga acatggatcg
      601 acggctcctt taccagactg actcaggctt atatatggat tgccgttttt tatcaatgtt
      661 tttcaccagc ggcggacctt tccgccgggt ccgtccatca aaccggagac gaaccctttt
      721 ttgcatcttg acccggtcgg gcctatccgg taatccatgt accatgaaac tggaagaaca
      781 aattgccgaa ttgaccaggg caggctcagg caccctgtgg gaaccggcgc gcctggccgc
      841 cgttgtgctc gccgcgtccg ccgcgctggc cggcgctgtt acccgcaatc cgtttttttt
      901 gggaatcgcg ctggctttgt cgatcctcgc gtttgccgtt cggcagaccg ccccgcacat
      961 tgccaacgcc gtgagaggtt tacgcgaagg cgtgaagcag ccgggtacgg tcgagatttc
     1021 tcttcatcaa tggaccgatg cggaatcgaa tgttcatgag tcctttcatg gggtgatttc
     1081 catggacggc cagccggcgt gggagatgga atttgtgacg cctcaaaact ggcagccccg
     1141 ggaaggcggc tttcaggccg agctggcttt tattcgcggc gtggaatggc cggtcgcggt
     1201 cctgacgcgg gaaggcctgc tgtatccccg acggaaaccg ttgcgcgcag gccgcccgcc
     1261 ttcttctcaa tccggctgaa caacggcccg gtttgtgaac cggttgaata tttgcggaca
     1321 atgttttata aaagcggcaa aagcgaggga gtggcggata tgccgattta tgaattttat
     1381 tgccgaaagt gcaatacgat ctataatttc ttttcccggt cggtcaatac cgacaaaatt
     1441 ccctcctgtc cgaggtgcaa aaacgtgaag ctttcgcgcc gggtgtccct tttttcgacg
     1501 attaccggaa gggccaaaga cggggaagaa ccggatcctt tccggggcat ggatgagagc
     1561 aaaatggaaa aagccatgat gcagcttgcg gcggaagccg aaaatattcg ggaggacgat
     1621 ccgcgcgccg ccgccgacct gatgcgcaaa ttgtcggatg ccgccggcat gaagctgggg
     1681 gacagcttcc aggaagcgct caggcgcctg gaggcgggcg aggatccgga taagatcgat
     1741 gaagagatgg gggaacttct gtcggcggaa gatccgttcg gggaaaaaaa gaaggccgga
     1801 aagtccgcgg tgcgaaagcc ccgcctcgat gaaaccttgt atgatcttta gggcatgaaa
     1861 agcgtcggga ggaaaaccat gagagttctg acattcaggg tgaccttggt ggttctgttt
     1921 tttctctgct gcggttgggc gtccgcggcg gtccggattt cacagctgcc cccgccgcct
     1981 cctacggcga agcttcgggt ctttgtcgtc gcggtgacga cggaattcaa atccgcgaaa
     2041 ggccctgtct tctggccggt ttcgccggag gaatttgatc gaattcaaaa aaaggccatc
     2101 ggtgagcggc tccgtcagca gggcatttac gaagtggtcg ccgacagcga ccttcgggcc
     2161 gctctcggga ctcaaaaacc tgcgggctgg gagtggctgg ccgatgactg ccggctggtc
     2221 cgtgaggtgg gccgtgccct tcatgccgac tacgcgctgg tttccgagcg cagtttcgcc
     2281 atccacctcc agttcgacac gcggctgatg aatttgcatt cgggaaaaga gtttgccgcg
     2341 gaaggctacg ttccgtccac gatgctgcgg gcgatgaaca aagagcaaaa aacgcttgcg
     2401 ggcggagaag cggtaaaaat tcaattccgc cagatctttc aggaagccag gggcgacctg
     2461 atgagaacag ccgtccgcaa aggaaaaatc gcgacgaaag gatctgggcg ggacgaaaag
     2521 acggtcccgc cgcctttgga ggaaaaggca gccgctggat cgcagcccct ggaaaaagga
     2581 ctgcaggccg cgcaggcttc aaaagacaag gccccggccg ctacgcttca aaagacgccc
     2641 ccgccccctg ccgcggtcga aaagcaaagg gcgtttgaaa aggagctgga gcgggcgctc
     2701 aaccccgggg atcagaagaa ggcagggccg cgactgatcg tttacgattt tgaagcctcg
     2761 gagccgatga aggtagcggg cctgattctg acggaggcac tgcgcgaagc attgcaccat
     2821 tccggaaatt tcgtgctgat ccagagggaa aacatggtga aattcctgga ggagtataaa
     2881 ttacaacagt ccggtctggt ggataaaaag caggccgtcc gggccgccaa atggctggcg
     2941 gccagcgagg cggtgacggg aaatctggcc gtgctgggaa acaccagcct cctgcaggcc
     3001 aaacggatgg atattgaaac cctgggaacg ctggccgcgg gatcgatcaa gtgtccggcc
     3061 ggccgcgaag atgaactgct cgaacggatg ccggagctgg ccgcgatgct gaccggggga
     3121 aagaaataaa atcagcggct cttcggcgtt tattttttga acagggcgcc aagcttttcc
     3181 tgaaggtcgg ccggccgccg gccgggtttt gccgcgccgg tccggtattg aaagtagtcg
     3241 cagaaattgg agcggctttt ttccacaacc cgctcggcct ggctttcccg gcagtcgttg
     3301 tagatccccg gttcataaaa gacgcagttc aggcagatgt gcagatccgc tccgcagaag
     3361 gggcattgcg cctgccggcc tgccggttcg ccttcgggga tttctttttg acagtgagcg
     3421 caacatttca tggcgcgcaa aaatacaggc ggagcgtgtg gtttgtcaag agactttccg
     3481 gtgccggtcc atttcaacgt gaaattccgg tccggatgtg ataggacagg cgtgattttt
     3541 gggtgaccag gcggaggcgg ttatggcgga tcggtcaaaa ctcggcatgg agtttcccgt
     3601 ttattccatc cgggtggaaa aaaacaaaat cgcggaattt gtcgcggcgc tcgagcagaa
     3661 ggaagatacg gagcagattc acccccatta ccgggatgcc gatgccgcga aaaaggcggg
     3721 atatgcaaac atccccacgc ctcccacttt tcccaccagc catgtcttct ggacgggcgg
     3781 cggtcttctc ggcacgatca aagccgtggg ggccgatctg accaagctgc tgcacaccaa
     3841 ggaagaatac gagtattttg ctccggtctg cgcgggggat gtcattaccc gcagcatgaa
     3901 agtggtggag atgtacgaaa gagggaaaaa ggagcagaaa agcaggtata tgcaggtgac
     3961 cgtcctgcaa acggacatga tcaatcagca cggcgtgctg gtcatcaggg cgcgcacgac
     4021 cttcatggag agaggctgat cggcatggcg ctggtttttg acggaattca aatcggtgac
     4081 atcatgccgc cctacacgtc cgctcccatc acgcgaacgc acctggtgcg ctacgcaggc
     4141 gcttccgggg acttcaaccc cctgcatcac gatgaaactt tcgcgaaggc ggtagggctg
     4201 gagacggtca ttacccatgg catgtttatc atggggatcg cgggccaggc catcacttcc
     4261 tggatcgaca atcgatggtt aagaaaattc tccgtgcatt tcctcagcat gacccagccg
     4321 gtcgatctga acgattattc caacacaaaa gaccgggcga ccatcacggt gaccggcaaa
     4381 gtagtggaca aatacgaaga aaacggagag acaagaatcc gctgcgagct gacggcccgg
     4441 gacgcgctgg gcagcagaaa gctcaagggc tatttcgtgg cggccctgcc atgagctttt
     4501 cccttgtcaa tggccgcagg tgttttttat gaaagcccgc atagtcgtga ttgatgatga
     4561 tgccgtcgcc tgtgaatttc tccaggaagc gctccagcgc gaaggctacg acgtcacagc
     4621 ttttacttcc gctccggccg cactgacgct ggatctttcc gactatgatc tgctgatgtc
     4681 cgacattcgc atgcccggaa tggacggcct gcaatgttta agcgaaattc agaaaagatg
     4741 gccgaatctg cccgtgattt tgatgaccgc ctacggttct ctggaaacca ccatggaggc
     4801 gatccggctg ggagcgtggg attacatcag caagcctttt tcgccggaag actgccgcgc
     4861 gctggtgaaa aaagtgctgg aggtgcgcga gctgcgcaaa aagcggttga tcgagcccgc
     4921 ggaagacgcc gatgaaacca agctgatcgg ctcttcgccc gtcatggtgg agttctacaa
     4981 gcagattgcc cgcgtcgccg attcccaggc gagcgtgctc atcgaaggcg aaagcggcac
     5041 cggcaaggag ctgaccgccc ggtcgctgca ttccatgtcc tcgcgccgcc agaagccttt
     5101 cgtcgtcgtg cattgcggcg cgatccccga caacctgctc gaatcggagc ttttcggcta
     5161 cgaaaaagga tcttttaccg gcgcggatca tcagcatgtg gggctgattg aatccgcctc
     5221 cgccggcacc atttttctcg atgagatcac ggaaatgtcc acggcgctgc aaagcaagct
     5281 gcttcggttc atgcaggacg gcgaggtccg ccggatcggc ggacatgatg ttcgccacgt
     5341 cgcggtgcgc gtcgttgccg ccgccaataa aaatgtcgat gaagaagtcg ccgccggccg
     5401 gttccgcctg gatcttctgt atcgctttat cgtccgcctg cgcattccgc ccctgcggga
     5461 gcggcggccg gatattcctt tgctgatcga aacgatgctg aagaaactgg gctatcccga
     5521 tgttcgggtt tcaccggaag ccatggaaac cctcatggcc tatgactggc cgggcaatgt
     5581 gcgcgaactg gaaaatgttt tgcggcagac gctgctgctt tcgccgttcg tggtcatttt
     5641 gccggaaaat ctgccggacc ggttccggac ccgggcggcc gtcaaagcgc aggccgacgg
     5701 gccgcaatgg acgcccctgg aagaagccga gcgtgagcag attgttcaga ccctgaaaaa
     5761 gacggactgg aaccagtcca gggccgccga gattcttggg atcgaccgca aaaccctgcg
     5821 cacgaaaatc cgccgctacg gcctgcagga aaaataaaca tcaatccgcg gcggcgtcgg
     5881 ccatccgcag gtacaggacg accgtcgtgc cgctttccgg tgtgctttga atgtccatgc
     5941 ggccgttatg ggcccggatg atgtcccgga caatggccag ccccaggccg cgcatgcctt
     6001 ccccctgacg agagctgacg aaggggtcgc agactctttt cagaagatcc gccgcgatgc
     6061 ccggtccgtt gtcggcaatc gaaatggcga tggtgcgggc cgcccggtca tcttcggccg
     6121 agaggcggat gacgccgccg ccggacatgg cctcgatgga gttttgcagg atgttcaaaa
     6181 gcgcggtttg aatccggtag cggtcggcgt acaaagacgc agcggaccgt tccagccgca
     6241 cctcgatggc gatccggttt ttttggatca ggggctccac cagcggcagc gtttcactca
     6301 gaatgtgatg gacggtcatg cgctccagat gcggttcggg aagacgcgta gccttgcgga
     6361 cattctgaat gacgtggtcc agccgctgcg tctgttcgac gatcacgttc agcttgtgcg
     6421 ccacatccgg cggccagcct tcccgctcca gaagcagccg ggccagtccg gcgatggaat
     6481 gaagcggcgt tcccagatca tgggccagat tggccgtcag gtgccccagg gccgtcaact
     6541 gcttcaagtc atccacctgc ttctgcaggc cgatcacgtt gagcgtctct tcccggacgc
     6601 gatcggccag atggcgggtg aggttgtcat tgcgctccat gacgttttca atttcatcgg
     6661 ccattcgatt gaaatgatcg gccagaagcc cccactcgtc acggcggccg gcgggaatgc
     6721 gttcgacgta ccgccctttc tggaattcat cgatggtgtt gatgatcgtc tccagcggcc
     6781 ggccgatcag ccagccgtag ctcaggccca aaagcaggag aatggccagc agaagccccg
     6841 cgctgctgac cagcaggagc cgccgcgcgc ggctcatgat ttccgcgttg gcccgggaaa
     6901 aagcgatgat gccgaccacg acgacgcggg agtctttgga gtcccccggg acgggataca
     6961 gcaggcccag tgaaggtccg tcctccgttt ccagtttcat ggaaagggat tttccctttg
     7021 cgccggtttc cgccacgaga tggtccggcc attcgtaggc tacgcggctg gcgccggcaa
     7081 cgtaattgac ggaaccgcgc cggatttcca gaatatcgat gcgggccagc cgcacatcgg
     7141 tctggatgtg atctttcagc gtggaatcga gcgtggcgat gccctgccgg gtcggaaagt
     7201 gctgataaaa ggtgctgacg gtttcggcga cgccttccag gcgctttccc tgctggtcca
     7261 ccagaatccg gtcaagggcg gtcaggtgaa tcagccccca tacggccatg gtcagcgcca
     7321 ggccggccac ggtcagcgca accagcctga cgcggagcgt caaatggttg ttgtaatcga
     7381 taatttttga cagcagtctt ttgagcatgg aaaaatgtat aacaaaaagg cgcgaaataa
     7441 accacgtgga gttcgagcga tcccgaaggc ttcccgcggc gggggccggc ataaaaaaag
     7501 gggcgggttt ttcccgtccc tttttttgtc aatgccgcaa aaacgtatat cgcccaggcg
     7561 gcttatttgg ctttcttggc tttgcgctgc gcttcgatca gggctttgtc ttcatcggaa
     7621 atcttggtgg ctcccgactg cacttcttca accgtcgaga cgcctttttt ggcttccggc
     7681 gccttcacct tttctttttt gaccaggccc gcttttttcg cggctctttt ttccttggcg
     7741 gctttctgcg cgtctttcac ggcggcttta tccgcgtcgg aaatcttggt ggctcccgac
     7801 tgcacttctt caaccgcgga gacgcccttt ttggcttccg gcgccttcac cttttctttt
     7861 ttgaccaggc ccgctttttt cgcggctctt ttttccttgg cggctttctg cgcgtctttc
     7921 acggcggctt tatccgcgtc ggaaatcttg gtggctcccg actgaacctc ttccaccgtc
     7981 gatacggcgg gcttgtcggc ggccatcgcc atggtggcga atgcgaaaac aactgccaca
     8041 accagtaatg ctaaacgctt cattgtacat cctccttttt aatagtttta aaagaaccca
     8101 tcaattttca gggactcctc accctctgtc accaggcaat cgaccttatt ttgcagtggc
     8161 tgccgatttc tcctttacct gttttgtttc attttttcta tgattttttt cggccttcgg
     8221 ctgggctatt ctggaagcgg tcatttttcc gtctttgatc gtgtaatcga ttttgacaaa
     8281 atcattcacg gcgatgcccg gagccgggtt ttccagggcg aactccatga tttccgcgct
     8341 tcccttgatg cttcgttcaa tccgtacggc cttgtcggaa atttccacaa ccttgccccg
     8401 ggcgttcatg gtggctgttt tggaaacaac cttttgcgct gaatgggcgc ccggtttctc
     8461 ggccgcgaaa gcgaacacgg ccagaaggaa gacaagcccg attgcacaaa ccaaaacttt
     8521 tttcatagag tgtcggcacc ctggttattt ttgcttttgt ttttttgagc aatgactatg
     8581 ccagtttccg taccgatctg tcaacctcta ttcatttgga atgacggtta aatttttaaa
     8641 agagggtctg ctcctttcat attggaccgg cggatgagga ataacgcccc aatggatggg
     8701 gtaaaatgcc tcagggcagt ctgcggtaca tccggcactg caggtaaaag aaaaaggttt
     8761 cggccccgcc cagcgcgagc agttttttgg cgacggcccg cactgttttt tgtccggaaa
     8821 ccttgtggtc ggacaggtag aggctcagaa gaccgctgat cagcaggtga tgaaatttcg
     8881 cgtcgggcag atccgccact cccgcctgcg ccctgcggta aaaatgaccg agtctctgcg
     8941 tcagccgttc atgattttca tagtaagaac aaaaattcag atcaccctcg cgccggtcct
     9001 cctcctggtc gatcagatag tccagaagaa tatgcaggcc ctggacccag gggaaatagg
     9061 ccctgcggat ccgttcaacg gcgggcgcgg catcctgctt tccgaacagc agcgagacca
     9121 tgcagaaaat ccccagcgtg gagccggcgc aggcggcgaa ttcataccag gccatttcag
     9181 gaagaccggg cctgaggccg tcaaaccact tcttcaggcg ctcctcgcgc tcgtcgggac
     9241 gaacatgctt gtgcacctgc aggtccacgt aaaggccggc cagttcccgg atgaacggcg
     9301 cgaccctgtg gtattccggc aggccgccca gaatcatttg gcaggtccgc accagttcgt
     9361 ccagatagcc tccgtcattc cgctcgggcc ggaaccggta ataggcggca agcggcgcct
     9421 ccggcgtcag ggcgtgcagc atggcttcat gcagcgcgcg aaaatcttcc gggtcctgcg
     9481 aggtgctgcg gtcgcacaga ttgtccaggt aatcgctgat ggtttgatag gcgactataa
     9541 attcaatggc ctcacggcag aagtgtccgg ccgtgaggcc gcagagagaa cctccttcgc
     9601 agtgaaatgt cttggcttca atgctcgcca gagcctggcg ccgcagttca ggatcgggaa
     9661 tccggcgcgc gcgctccttc cagccctgca ggaaagtccg cgctgcggga cggacctgca
     9721 gtatcattct ggcggtcaat gatagaatac gggttggcgt ttgcaaaggt cgctcctgag
     9781 aaggaagttg ggccggtaaa tctttagcac aggccggttt ggttctcaac aggctgttgt
     9841 cccatgcggc tgctcaaagg atatgatttg ttccggaccg ggcaaatcca ttgaccgttt
     9901 tcctgcatct gtgatagcgg tgtcgccaag ggagataccg tgatggatcg gacggcgatg
     9961 gaggccgatg ttgtggtggt gggcggaggc gcttcggggt tgatggccgc cggacgggcg
    10021 gccgaacggg gcgcccgggt gctgcttgtg gaaaaaatgg aggggtgcgg caaaaaaatt
    10081 ctggtcagcg gcaaaacgcg ctgcaatctg accaacgccg cggacctgaa atcctttgtt
    10141 tccatgtacg ggcccaacgg ccgttttctg cacggcgcgt tttcacgctt tttccgcccc
    10201 gaactgctgg cgttttttga acggtacggt ctcgtgacca aagcggagcg gggcggcaga
    10261 atttttcccg tttccgacga cgcccgcgac gtgctgcagg tttttcatca atacctggaa
    10321 gagcatcatg tccgcagaca gctccatgcc cgggtgactt ccgtggcgct tcgcccggat
    10381 tccctctttg tcgttgagac aacgggcggc tcctgtctgt cttcctcggt gattttcgcc
    10441 accggcggcg cctcctggcc ggctaccggt tcggaaggcg acggctatgc cttgagcgcc
    10501 gcgctgggtc acaccatcgt caaaccaaga ccggcgctgg ttccgctcgt cgtccgggaa
    10561 caggcgcttg cccggtcgat gcagggcgta tccctgcgca atgtccgcgc cacggcgttt
    10621 cagggagaag ccatgaccat agacgatgcg ctcactccga cgtacgtttc cggccgcggc
    10681 gcaagcaaaa ccccccgtcc gccggtcatc gaaagccggt tcggcgaaat gctgtttacc
    10741 catttcggcc tgggcgggcc gatcattctg cttttgagtc ttgccgtggt ggacgccctt
    10801 gaacaggggc cggtttccat tttgattgat ttaaaacccg ccgtcacccg ggagcagctc
    10861 ggcaaaagac tgcaaaaaga tttcaacgcg ttcggcaaaa gaaaaacagc gacgatctgg
    10921 aaagactatc ttccggcaaa gatgattggc ccgctcgcgg ctctttccgg cgtggacgaa
    10981 aacaagccgg cgcatcaggt tggcgccgcc gagcggggga aaatgatcga cgtgctcaag
    11041 gcgctgcggt tcaaccttca ggccccgctg ccgctggcca aagccgtcgt gacggcgggc
    11101 ggcgtttctc tcagggagat cgatccgcga acgctggcct cgaagaaagt tcccggcctg
    11161 tttttctgcg gcgaggtgat ggacatcgac gcggacacgg gcggctataa tctgcaggcc
    11221 gcattttcca ccggatacgt ggccggagaa agcgccgccg cctttgccgc cgcaaagaaa
    11281 cgcccctgaa tccggcggcg gatcacctgt cttgcccggg attttcacga ggggacgaag
    11341 attccggctt gccggtcgga ggggaaagga tgcttacgat ttttcaataa attcccggac
    11401 cttgcgggat aattccgcca tggtgaaggg tttctggata aagccccggc agccttcggc
    11461 gatgagttcc ttggcctgac cgtccaggct gtagccgctg gagagcagaa cgttcacggc
    11521 gggattccgg cttcgaagcg cctcaaaagt ttccctgccg cttaaatccg gcatgatcat
    11581 gtccagaata accaagtcga ttatttccgc gttccgtgca aaaacatcaa cgcccttcct
    11641 gccgccgccg gcggtcagga cgctgtatcc cagcccttcc agcatctgac tgccgacatc
    11701 cacaatcatt tcttcgtcgt cgatgatcag gatggttccc cggccctgcg cgggcctgct
    11761 ttcctgctga tcttcctcgt cggctttttt ctcagacgcc ggaagatgga tgatgaagct
    11821 gctgccggtt cctttttcac tctccgcgct gatgaatccg ccgtgatttt tgatgattcc
    11881 gtaaacagag gccagaccaa gccccgtgcc ccgcccccgc ttcttggtgg aaaaaaacgg
    11941 ctcgaaaatg cgcaatcgcg tggcctcatc catgccgatt cccgtgtcct tcaccgtcag
    12001 cttgacaaat cttcccggct ggacgccgaa gcccgcgaca tcctgattct ccagcgcggt
    12061 gttttccact gaaatatcca aatggcctcc gccgggcatc gcctgccatg cgttcacgaa
    12121 aagattgagc aggacctgat ccatttgtcc gcggtccaca tccacatacc ataggtcttt
    12181 ttccgcctga tggtgaatgg atatttcttt tctggttctg ccgaacactt ccgcgcttcg
    12241 ggcgacgaat ttgcccaagt gagtcgtccg gacctcgtac ttgccgcccc gggcaaagcc
    12301 caggagctgc cgggtcagat tggagccttt ctggacatat tcttccatgt tcttgaggcg
    12361 ttcgtggaag ggatgctcct gctccatgtt catgagcatc agggaaatgt ttccgagaat
    12421 gcccatcagc agattgttga aatcatgggc gatgccgccg gccagcgtcc cgatggcctc
    12481 cagcttctgc gcgcgcagaa gctgttcctc catggctttt ttctccgaaa tatcaatgag
    12541 aaacccccgg aaacccgccg gtttcccttc tttgaaaatg cgggaggtgt gcaccatgac
    12601 cggaaaccgc gccccgtctt ttttctgcgc gatgtattcg ttgagtccga gagattcccc
    12661 ccgaatgatt cgttgaaagt tctcccccgc ccgctcccgg tcttccgggg ccagaacatt
    12721 gaagagcgac atgccctttt ccacatcgcc gcgggtgaaa ccgaaccggt cgaaagaagg
    12781 ttcgttgaca aaggtgacgg tcccgttcaa atccgcctca aagacggttt ccggcagcag
    12841 gcgggccatg tcccggaacc gctcttcgga ttgccgcaga tcgtcctccg cctttttccg
    12901 gtcggtgatg tcgaacaggg aggccacgct gttggttgtg cccggaatca tttcgacgct
    12961 gaggaagaaa gtccgaatgt ctcccgatcg ggtcggaccc cggaattcgt acgtgccggg
    13021 aacggattcg gggttggttc ttctggtctt gtgccgctcc accatgaccg ccagatcttc
    13081 gtcaacgaca aatctggtcc aggacatttt gttttcaact tccttcttgg aataccccat
    13141 ggttttttcg aaattggcgt tcaccgacag gacggtcgta tcctcggcca ggatcatgtt
    13201 ggcggtggcc gtgtgttcaa agatcgcccg gtaatactcc tcgctctttt tcaaagcctg
    13261 ttcggttttc ttccgctctg aaatgtcgcg ggtaatgccg aggatgcctt caggacggcc
    13321 gtggtcgtca tagagcagcg acgccttgac ttccgtccag atcgtcgaac cgtccttgcg
    13381 gagcatttcc acttcgatgc tttgcgtcca ctcccgggcg tggcgttgcc cctgcgtttc
    13441 ttgggagaat tccctgctga agatgtccag gaccttctta tgcgaatccg gcgtcagaaa
    13501 ctcttccagg gactgcctga cggcttcttc ggggggatag ccccgcagac gttctacgga
    13561 agggctgaca tacgtatgct tcaactgcag atccagcacc cagatgacgt ccgtcaggtt
    13621 ttccgccagc aggcggtagc gcttttcatc attcccgatg gttcgatcga aaaaattttc
    13681 tttcgaatcc gaacccctgg ttttttttgg cgacgctttc ttcgcagtcg gcttgctcat
    13741 ctttttcccc gaaagaaacc ggacggttga caatattcac gcccgacgga cgctccttca
    13801 ttgaaacctg tcaccattaa actgattttt caactttttt ccattgtttt gtgaaaccgg
    13861 atttggggcc attcttccat ggtgtgggcg agccgccaat cggagagcgc cagataaacc
    13921 aggtttcctt ccgtgtccat cgccaggctc atcagatttt ttttctggaa ttcttccatc
    13981 tttttcgcgt cgtcgcacgt cacccagcgg gcggcggtat aatcggcttt ttcataatcg
    14041 gcgtaaacgc tgtattcgtt tttcaaacgc gacatggtta cgtcaaactg cagtgagccg
    14101 acggcgccca ggatattatc cgacccccac aggggcttaa agacctgaat cgccccttct
    14161 tccgaaagct gggtcaaccc tttttgcaaa tgtttcagtc tcagcgggtc tttgagccgc
    14221 acccggcaga aatgctcagg ggcgaaatga ggaatgccgg taaacttgag cggttctttt
    14281 tgcgtgaaag tatcgccgac tttgatcgta ccgtggttgt ggataccgat aatatcgccc
    14341 ggataggctt cctcgacgtt tgtcctgtcc tgcgccatga atatcgtagc gttggaaagg
    14401 gtgacttccc tgccgatgcg atgctgcatc acatgcatgc cgcgttgaaa ctttccggag
    14461 cagatgcgca aaaaagcaat gcggtcgcgg tgtgccggat ccatgttggc ctgaatttta
    14521 aaaacaaagc ccgaaaaatc atcttcatcc ggatcgacga tccgtgtggt ggtggggcgc
    14581 gggacaggag gcggtgcgat ttcaacaaaa gcatcgagca attctttcac accgaaatta
    14641 ttgatggcgc tgccgaagaa aaccggcgtc tggccggcct ttaaatattc cttgatatca
    14701 aaaggattgg ccgcgccttc caagagagcg atttcctcgc gaagttctcg ggcttggctg
    14761 tccagcaatt catcaagtaa tggatccgac aaatcgttga tcacaacccc gtcttccgag
    14821 cgcctcgctt tacccggcgt gaacagatgc agctttttgt cgtagagatt atagacgcct
    14881 ctgaagcgtt tgcccatgcc gatcggccag gacagaggcg agcattcgat ctgcaatttg
    14941 tcctcaatgt ccgccagaat atccagcggc gccaggcagt cgcggtccat tttattgatg
    15001 aaggtgatga tgggggtgtt gcgcaggcgg cagacttcca tgagcttttg cgtctgcgtt
    15061 tccacgcctt tggcgccgtc gatgaccatc agcgcgctgt ccaccgcagt gaggacgcga
    15121 tacgtgtctt cagagaagtc ctggtgcccc ggggtgtcca gcagattgat ttcgcaatcg
    15181 gcatattcaa acttcataac ggacgaggtg acggaaatgc cgcgttcctt ttcaatggac
    15241 atccagtcac tcaaagcgtg ttttgatgct ttgcgggctt ttaccgcacc ggccatctgg
    15301 atggcgccgc cgaaaagcag cagcttttca gtaagcgtcg tcttccccgc atcgggatgg
    15361 ctgatgatcc cgaaggtgcg ccttcgttca atttcttttt gcagttgctt gctcaaaata
    15421 ccctcatccg tgacgggagc ggttccaacc gttcaccaca aaattccctt cagccttcag
    15481 tctgctttcc ctaaacggtg ccgcctctta acgaataatc ccgggactgt caaccgataa
    15541 gacaccgata acaccgcgaa tccggttgcg gcccgggcag ccgcgcgcaa aaactccttg
    15601 acaaaaaggg actgttgcat caaagacttt tgccgtgagc gggcgtaatt cagtggtaga
    15661 atgtcagctt cccaagctgg acgccgtggg ttcgagcccc atcgcccgct ccattttacc
    15721 catttcttcc ttttcttcct ttaatcagtg ctttaaatag tgcttgcatt ggctgttggt
    15781 gtatggtagt ggcatttcaa ccatcactga aagtcgtttg ctgaagtggg ctcccgccca
    15841 cttttttatt ttcggaagat gcggctatgt acgatgaatt gatagataaa atatggcggc
    15901 tggctgagcc ggtcatcctg tcggaagaca tggagctgat tcacgtcgaa tgcgtcaaaa
    15961 tgcattcccg ctgggttttc cggttttttc tggacaagga aggcggcgtc acgctggacg
    16021 actgcacggc ggtcagcaat cagcttggcg atattctgga cgtgcatgac ctttcccgcg
    16081 ggccttatac gctggaagtg tcgtcgccgg gcccggaccg tccgatctct cgcgatcggg
    16141 attttgagaa attccggggc gccaaagtaa aaatcaggac ccacgtgaaa atagaaggcg
    16201 tcaggaattt tcagggaacg ctcatggatt acgccgaaga aaacggccgg aagcttgtcc
    16261 gcatcggcat ggcggaccgg attttcagca ttccgagaaa cgatatcgcc agggccaatc
    16321 tggtggacga ggataccgtc aatgccttgt gaaaaaaacg ccgacaagct caacagaacc
    16381 gcagccgcaa gagaaataag ggaggattgg atcaatgttg catgacctta aacgtctgat
    16441 cgagcagatg gggaaggaca gggggatcga caagcagatc atcacggaag ctctcgaggc
    16501 cgccatgctg acggcggcgc gcaagaagct ggggcaggac gtggaaatcg aagcccatta
    16561 caacgatgat gccggagaaa ttgaagtttt tcagtttaaa acggtcgtgg acaaagtgct
    16621 tgatcctgaa acgcagattg cccatgagga tgcccggcga accctggatg agggcgcgga
    16681 gccgggggat tccctcggga tgaagataga aaccagttcc ttcgggcgca tcgccgtgca
    16741 gatggccaag cagattatca ttcagcgggt caaggacgcg gaacgcgaca atatttacga
    16801 tgagtacaag gaccgcaagg gcgagctgat caacgggttc gtccagcgtt ttgaaggcgg
    16861 caatattatc gtcaacctgg ggcgagccga gggcgtggtg ccgcccatgg agcagatcca
    16921 ccgcgaaacc tacaagcgcg gcgagcgcat ccgctcctat ctgctggagg taaagaagaa
    16981 cacgaagggc gcgcagatca tcctgtcgcg cacgcacccc gcttttctga aagcc
//
LOCUS       DAOSIM010000019        15485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_3972_length_15485_cov_8.040830, whole genome shotgun sequence.
ACCESSION   DAOSIM010000019 DAOSIM010000000
VERSION     DAOSIM010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 15485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15485
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3972_length_15485_cov_8.040830"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            285..1085
                     /locus_tag="PLR47_01835"
     CDS             285..1085
                     /locus_tag="PLR47_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOG11704.1"
                     /translation="MRKALYPTILVLFLMLCLVSAAFAGPTIDSILKKKELVVGTSAN
                     LPPLSFKAKDGSLKGLDIDLGKMIASAMDVKLRVVELPFNELIPALESGKIDMILSCM
                     TITPERNLRVVYVGPYFVSGQSILTTRDVAMNINSMDDINKSDFSIAVPAGTTTEKIA
                     KALLPKAKMVVAGSTEETLDLLRKKKVKALMADYPYTSVTSFKYQDKGFVANPPFSAE
                     PIGIAIRPDDPLLTNFLENLLSLLKGDGMLDGLNKRWFSDPSWISELP"
     gene            complement(1156..1470)
                     /locus_tag="PLR47_01840"
     CDS             complement(1156..1470)
                     /locus_tag="PLR47_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11705.1"
                     /translation="MTEILFDADEDWEKRVLCSDEACIGTIGPDGKCRECGKPYEGAL
                     PESHGQKTNPPPAPKEEQPAVPEDDASGEDWEKRVLCGDEACIGVIGPDGKCKECGRP
                     LQ"
     gene            complement(1543..2985)
                     /locus_tag="PLR47_01845"
     CDS             complement(1543..2985)
                     /locus_tag="PLR47_01845"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOG11706.1"
                     /translation="MSEIKLEKLDDNRWLLPRTGAMRVPGIIYANEKISGLLTRDESA
                     KQVANVAHLPGIVDFSLAMPDIHWGYGFPIGGVAAFDMDDGVVSPGGVGYDINCGCRL
                     LATELTLHDIKDNVKDLTTALYRRIPTGVGSTGYVRLSGKDQKKVLEEGAGWAIKAGF
                     GSGQDLETTEDGGCIGGADPEQVSARALERGKQQLGTLGSGNHFLEIEVVEEIFDEKA
                     AQAFGLRKGQVVVMIHTGSRGLGYQICDDYLALMVKHQHETGITLPDRQLACTYLSSA
                     RGQNYLAAMACGANYAWANRQILMHLTREVFEKSLRVAPRELGMRLVYDVCHNIAKIE
                     SFPLAGVEKKLCVHRKGATRAYPAGHDAVPARYRSVGQPVLIPGDMGAGSYVLVGQEK
                     AMQETFGSACHGAGRVMSRTQAIKSSRGRSISRELAEQGVLVMAGSKGTLSEEMPEAY
                     KNLNDVVGVVHDAGIAKKAARLKSIACIKG"
     gene            complement(2978..3409)
                     /locus_tag="PLR47_01850"
     CDS             complement(2978..3409)
                     /locus_tag="PLR47_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOG11707.1"
                     /translation="MKKYELFDHTADLGLEIYGRTKRELFANAALALFDVMIQDVDLP
                     TKRGGKERVKTRTVQGADAGDLLVNFLRELLYWFNGMGRVLNRVSILSCGSKKLVVQC
                     VVEPYHSERHLIKTEIKAVTYHGLSVEKTKNGWKARVIFDV"
     gene            complement(3496..6015)
                     /gene="gyrA"
                     /locus_tag="PLR47_01855"
     CDS             complement(3496..6015)
                     /gene="gyrA"
                     /locus_tag="PLR47_01855"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOG11708.1"
                     /translation="MERDIHHRQTLVNIEDEMKKSYLDYSMSVIIGRAIPDVRDGLKP
                     VHRRILFAMSEMGNRYNKPYKKSARIVGDIMGKYHPHGDAAIYDTLVRMAQDFSMRYT
                     LVDGQGNFGSIDGDPPAAMRYTESRLARISDEALADLEKDTVDFVPNYDESLTEPSIL
                     ATRLPLLLLNGTSGIAVGMATNIPPHNLKEIINGTIALIQNPKMIVEELMKYIPGPDF
                     PTAGFINGREGILSAYKTGRGIIRIRARALIEKNARNDRETIVITELPYQVNKATLIE
                     KISELVRDKKISGISDLRDESDREGIRVVVELKRDESSAIILNQLYKFTQMEVSFGII
                     MLAISEGRPQVFTLKAVLEKFIAFRQEVVTRRTQFDLARARERAHILEGYIIALNNID
                     EIIKVIKAAKSPNDAAAALCATFHLSEIQAKAILEMRLQRLTGLERDKIDAEYADVTA
                     LIAKLAGILADPQKVLKVIIEELQEIREKYADERRTEIVASTEDIDIEDMIVEEDMVV
                     TISHTGYIKRNAVSLYKSQHRGGRGKVGMGTKEEDFVEKIFIASTHHYLLVFTNMGKV
                     YWLKVYQIPQAGRAAKGKAMVNLVNLAPGEKVSATLPVKEFVSDKFIVMVTRKGIIKK
                     TDLAAYSNPRAGGIIAISIDDDDELVDVQLTNGDQDIFLATRLGMAIRFKEDDVRDMG
                     RTARGVRGINLDEGDDVIGVDIPAQGTFMLTVSENGFGKCTPIEEYRVQSRGGKGTIN
                     LKTVAKIGNVSGVLQVQGEENIMLISNAGKVIRLKVQEVPVNHRITQGVKLIDLEPEE
                     KLVGVARTTSEGDDKDDDVNGNGGDEANGSGEEKGPPADES"
     gene            complement(6031..8439)
                     /gene="gyrB"
                     /locus_tag="PLR47_01860"
     CDS             complement(6031..8439)
                     /gene="gyrB"
                     /locus_tag="PLR47_01860"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOG11709.1"
                     /translation="MVETYDADSIKVLEGLEAVRKRPAMYIGSTSKEGLHHLVYEVVD
                     NSIDEASAGFCDSITVKIRVDNSIVVEDNGRGIPVDIHKTEGVSAAEVAMTKLHAGGK
                     FNNETYKVSGGLHGVGVSVVNALSRTCELEVRREGIVYRQTYQRGVPDGPLHAVGKSR
                     GRGTTVAFLPDDEIFEETEFSFDVIANRLRELAFLNSGVKIALIDERADKKTEFIYKG
                     GIVSFVEYINRNRKVLHKEPIYVTGAREDCSVEVALQYNDSYLENIFSFANSINTVEG
                     GMHMVGFRAALTRVFNNYASANKIIKDGKESLRGEDLREGLACVISVKVRNPQFEGQT
                     KTKLGNSDVRGLVEGIVYEKIGSYLEENPSVARQILNKCMEAARAREAARRARELTRR
                     KSALEVGALPGKLADCQEKDPALSEIYLVEGDSAGGSAKQGRDRKNQAILPLRGKVLN
                     VEKARFDKMLQNEEIKVIVTALGTGIGTDDYDVSKLRYHKVIIMTDADVDGSHIRTLL
                     LTFFFRQMRELIERGYLFIAQPPLYKVVDKKKEFYIQNEEQMKNYILENGVNKVNLFM
                     EDGSPFPVSGSKLLTVIKKITRIKDLLDRFEKEHRDRNVVRIAAGDPTLTDETFQNEG
                     ELLKIAGRTVVALGSSVVADYRIDEDPDHGGYKMIFDIKTNGTIHTTCIDRETFRTPQ
                     FGEIRNLMGQVSVLGEPPYSDRQEEGAAPVILPTAKDLVDHIINLGKKGLTIQRYKGL
                     GEMNPEQLWETTMNPEKRTLLQVGVEDAVVADEIFITLMGDEVEPRREFIYQNAMYVS
                     NLDI"
     gene            complement(8490..9590)
                     /gene="dnaN"
                     /locus_tag="PLR47_01865"
     CDS             complement(8490..9590)
                     /gene="dnaN"
                     /locus_tag="PLR47_01865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOG11710.1"
                     /translation="MNRNIFLDGIQKTLGIVEKKTTTPILNNVLLKAEASKIKIIATD
                     REISLISDYEADIAENGEITLSAKKLFEMVREIQGDTVHFTKSANNIVKVTCQRATYK
                     IPGLPADDFPSVADSQQVEFFNIPGSVLKELISKTSFAMAFDESRKNLNGVLLETSSD
                     GKNHIWRMVATDGHRLAVARAQTADPCPEMAKGIIIPRKGLMEVKKLTDVHENVSVGI
                     HKNMFVVKTENTILKVNLVDADYPDYKRVIPAEKGVSVTLDKELFLHALRRMNVVSSE
                     RYSGVIISIAPDKMTLNSTNLDVGEATEEIDITYSGEEMEVGFNVNYVIDAVSAISID
                     RVVMEVGVGLKPTLIKPAEGDNYLCIVMPLKI"
     gene            complement(9755..11107)
                     /gene="dnaA"
                     /locus_tag="PLR47_01870"
     CDS             complement(9755..11107)
                     /gene="dnaA"
                     /locus_tag="PLR47_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOG11711.1"
                     /translation="MESVWDKATKIIQEKVSKQNFDTWIKPIKIVSMEDKCVQLSVPN
                     KFFKDWLMDNYLSMIKNTLHSVIGISVDIDFRLSKDKEKKPEVAALPFEQGKNQPPPS
                     PQRSKYFSFLNSNYTFDRFVVGPSNQFAHAASIAVGKQPAKNYNPLFIYGGSGLGKTH
                     LLNAIGLMTINAHPDLNVMYVSAESFMNEMINSIRYDRMQKFREKYRNIGCLLIDDIQ
                     FLAGKDRTQEEFFHTFNTLHDSGKQIVVTSDKFPKDIPNLEGRLRSRFEWGLIADIQP
                     PEIETKVAIIEKKMHEGKMELSPSVTHYIASHVESNIRELEGFLIRISAYSSLTNREI
                     DLDLVKEVLKTLVKHNNKEDVSIEEILKVVAGKMNVKISDMKAHNKNKNLVLARQISM
                     YLARKLTNFSFPDIGQKIGGRDHSTVIYANNKILKNIESDASFKKMIQDIEDSVIHKT
                     "
     gene            complement(11160..12017)
                     /locus_tag="PLR47_01875"
     CDS             complement(11160..12017)
                     /locus_tag="PLR47_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOG11712.1"
                     /translation="MAQKKDALGKGLGAIFSDLLETDAGRKAPVNTCGIEELRPNRWQ
                     PRKNFNDEDQKNLIASVRQSGIIQPIIVRRADSGYEIIAGERRWRAAQAAGLKEVPIV
                     VRKATDAEAAELSLIENIQREELGPLEEADAYVTLMETFHLSQESVSARVGKDRSTIA
                     NAIRLLKLPDRVKAALTEKKITPGHGRCLLSLNAAGEQTAALDVILKKGLSVRETERL
                     VNRLKQKPAEKKTAARDRFMADLEKQLTAKCMARVRLKGSSKKGLIEISYQSMDELNR
                     LARYLLDEL"
     gene            complement(12018..12785)
                     /locus_tag="PLR47_01880"
     CDS             complement(12018..12785)
                     /locus_tag="PLR47_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG11713.1"
                     /translation="MSKIICIANQKGGVGKTTTAVNLSASLAAANRKTLLIDADAQGN
                     STSGLGIDRMLYEKENFYHAMIGKAPLESVIVETALPNLDMVPSNQDLVGMEIEFVYL
                     ENRESRLRTLLQSLERSYEFILIDCPPSLGFMTVNALVAADTLIVPLQCEYYALEGLG
                     HLLNTVKLVKAGMNPNLALGGILLTMFDPRNTLSHRVTEDVRKHLGRKVFQTVIPRNV
                     RLSESPSHGLPIILYDIKSRGAIAYMELAQEILRGER"
     gene            complement(12877..13527)
                     /gene="rsmG"
                     /locus_tag="PLR47_01885"
     CDS             complement(12877..13527)
                     /gene="rsmG"
                     /locus_tag="PLR47_01885"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOG11714.1"
                     /translation="MILLQQGAKLLGVELNDRQLALFDLYRKELLQWNTRINLISKST
                     EREITARHFLDSLTAARFIPSESAGIVDIGTGPGFPGLPLKIAMPSIQLCLLESNRKK
                     VSFLKHIQRLLNLDGVFTLQDRTENIIRSDNWREKFDVAISRASLKLGDLMPLGEYFL
                     APGGLLITLKGREVEAELNAAFKDKKASQIYQLYQNDIDLEFLGPPRKIIILQKAK"
     gene            13576..14265
                     /locus_tag="PLR47_01890"
     CDS             13576..14265
                     /locus_tag="PLR47_01890"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOG11715.1"
                     /translation="MDNVKQPNHYNILFAENIPEMRGLWDGAVWRRAELLDVNCIRPE
                     GSDHRLRTQCKLLFDEQNLYGIFRVEDQYLRCVHTSFQSDVWKDSCVEFFVQPKGAGG
                     YFNFEFNCGGALLASYVTNPVRVNGRLQGCVPLTPNDDGQIRRIASLPSVVEPEIAQP
                     LVWCLEFSLPFALLAKYAGPFGAVTGQIWRANFYKCGNETSHPHWLSWRPLAGRNFHD
                     PSSFGQLVFDP"
     gene            14411..15154
                     /locus_tag="PLR47_01895"
     CDS             14411..15154
                     /locus_tag="PLR47_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOG11716.1"
                     /translation="MSLAAKTKNISIVLYKPKYPGNVGSVARAAKNMGIGKIVVVGET
                     GLDREEMERRSTHLAADVLDQIQYVASIEEALGGFHYIVGTTARLGKARGPFVSPRAA
                     AQKIADLSRKNKIALLFGPEDTGLANDQLRLCHSVVTIPTSREFTSLNLSQAAMILCY
                     EIFIASRAAETAAEAAPKLALSSELEGMYGQMKELLAKIGFLNPENPEYWMLDVRRFF
                     SRAGLLSREVKIIRGVCRQLEWYASNKKT"
     gene            15242..>15485
                     /locus_tag="PLR47_01900"
     CDS             15242..>15485
                     /locus_tag="PLR47_01900"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOG11717.1"
                     /translation="MRKIFSFTGAKKIVFGSGALAALAAHVREHHGQKPLVVIDQNLA
                     KTGLKEKISALLESDGIKFAFFDKVEPEPRIELADEG"
BASE COUNT         3211 a         4237 c         4066 g         3971 t
ORIGIN      
        1 cgccccacac tggatttgaa gtccaggagg cccaccagtg accttggcac ttccttcaag
       61 aaaatccaac gttccattac ctttaatttc ttcagactgt caatatctaa atgacttccc
      121 cgtccgtgaa gccttttttc gcttgacacg aaaccgccgc ccttctatac attccccaaa
      181 atttttcaaa tttcttttgc gggcgctttg cggattttac cgacgcagat gcgtgacggg
      241 aagacggtat cgcttcaaaa aaattcactg ataggagaaa ctacatgaga aaagctctgt
      301 acccaacgat tctggttttg ttcctgatgt tgtgtttggt gtccgccgcc tttgccggtc
      361 cgaccattga ttccatcctg aagaaaaaag aactcgtcgt cggcacgtcc gccaatctgc
      421 ctcctctgtc cttcaaggcc aaggacggaa gccttaaagg tttggatatt gacctcggca
      481 agatgatcgc gtcggcgatg gatgtaaaac tgcgcgtcgt ggagcttccc ttcaatgaac
      541 tgatccccgc gctggaaagc ggcaaaatcg atatgatcct ctcctgcatg accatcacgc
      601 cggagcggaa tctgcgggtg gtctatgtcg gcccctattt cgtgtcgggg cagtccattt
      661 tgaccaccag ggatgttgcc atgaacatca actccatgga tgacatcaat aaatccgatt
      721 tcagcattgc cgtgcccgcc ggcaccacga cggagaagat cgcgaaagcg cttctgccca
      781 aagcgaagat ggtggttgcc ggttcgacgg aagaaacgct tgatctgctc agaaaaaaga
      841 aggtcaaagc gctgatggcc gactaccctt acaccagcgt gacctccttc aagtatcagg
      901 acaaaggctt cgtggccaat cctcccttca gcgcggaacc gatcgggatt gcgatccgtc
      961 cggatgatcc gctcttgaca aactttttgg aaaatctgct gtccctgttg aagggggatg
     1021 gaatgctgga tggtctcaac aaacgctggt tttctgatcc gtcctggatt tccgaactgc
     1081 cgtaatttgc agataggaaa agaatagaaa cgccggaatg agcgcgccgg gttcagagga
     1141 accggcgcgc tatttttatt gcaggggcct cccgcattcc ttgcatttgc cgtccggacc
     1201 gatcacgccg atgcaggcct catcgccgca caggaccctt ttctcccaat cctcgccgga
     1261 tgcatcatct tcgggcacgg ccggctgttc ttcttttggg gcgggcggag gattcgtctt
     1321 ctgtccatgg ctctcgggaa gtgcgccctc atatggtttg ccgcattccc tgcatttgcc
     1381 gtccgggccg atggttccga tgcaggcctc gtcgctgcac aaaacccgtt tttcccagtc
     1441 ttcgtccgca tcaaaaagaa tttccgtcat gccgctctcc ggtttttgaa ttgatagggc
     1501 ttcttatgcc gggagcggca aaaagtcaaa tgccgcggat catcagccct tgatgcaggc
     1561 gatggatttg agacgagccg cttttttcgc gatgccggcg tcgtgaacca ctccaacgac
     1621 gtcatttaag tttttatagg cttcgggcat ttcttcgctc agcgtccctt tgcttccggc
     1681 catcaccagc accccctgct ccgccagctc ccggctgatg gaacggcccc ggctggattt
     1741 gatcgcctgc gtccggctca tcacccgccc cgccccgtgg caggcgctgc cgaaagtttc
     1801 ctgcatggct ttttcctgac cgaccagcac ataagagccg gcgcccatat cgcccggaat
     1861 cagcaccggc tggccgacgc tccgataacg ggcgggaacc gcatcatgac ccgccggata
     1921 ggcccgcgtt gcgcctttgc ggtgcacgca gagtttcttt tccactcccg caagggggaa
     1981 ggattcgatt ttggcaatgt tatgacagac atcatatacc agccgcatgc ccagttcgcg
     2041 cggcgcaact ctcagggatt tttcgaaaac ctcgcgcgtg agatgcatca ggatctgccg
     2101 gttggcccag gcgtagttgg ccccgcaggc catggccgcc agatagtttt gtccgcgcgc
     2161 ggaggataaa taggtgcagg cgagctgacg gtcgggcagc gtaatgccgg tttcgtgctg
     2221 gtgtttgacc atcaaggcca ggtaatcgtc gcaaatctga tagcctaatc cccgtgagcc
     2281 ggtgtggatc atgacgacaa cctgcccttt gcgcaagcca aacgcctgcg ccgctttttc
     2341 atcaaagatt tcctcaacga cttcaatttc cagaaagtga ttgccggaac ccagtgtgcc
     2401 caactgctgc tttccccgct cgagggcgcg cgcggaaacc tgctccggat ccgccccgcc
     2461 gatgcatccc ccgtcttcgg tggtttccag gtcctggccc gatccaaaac cggctttgat
     2521 cgcccatccc gcaccttctt ccagcacttt tttctgatct ttgccggaaa gcctgacata
     2581 gccggtggag ccgacgcctg ttggaatccg ccgatataag gccgtggtca aatctttgac
     2641 gttatctttg atgtcgtgaa gcgtcagttc cgtagccaaa agcctgcagc cgcagttgat
     2701 gtcgtagccc acgccgcccg gcgacaccac accgtcatcc atatcaaaag ccgccacgcc
     2761 gccgatggga aaaccgtaac cccagtgaat atccggcatg gccagtgaaa aatcaacaat
     2821 gcccggcagg tgcgccacat tggcgacctg tttggcgctt tcgtctctgg tcagcaggcc
     2881 ggatattttt tcattggcat agatgatgcc cggcactcgc atggcgcccg tccgcggcag
     2941 cagccaccga ttgtcgtcga gtttttccag cttgatctca gacatcaaag atcaccctcg
     3001 ccttccatcc gttttttgtc ttctccacgc tcaacccgtg atacgtgacg gctttgatct
     3061 ccgttttaat caaatgccgc tcggaatggt acggctcaac gacgcattgc acgacaagct
     3121 ttttgctgcc gcacgacaaa attgaaacgc gattcagcac ccggcccatg ccattgaacc
     3181 aatacagaag ctcccgcaga aagttcacca gcaaatcccc ggcatccgcg ccctgcactg
     3241 ttcttgtttt aacccgttcc tttccgccgc gtttggtcgg gaggtcaaca tcctgaatca
     3301 tcacgtcaaa cagggcaagg gcggcgttgg cgaaaagctc ccttttcgtc cgaccgtaaa
     3361 tttccaggcc cagatcggcg gtatggtcaa aaagttcgta ttttttcacg ggggaattat
     3421 agcgttgaac ggaaggataa acaaggaaga atggaaagcg ggcagcggcg ttggaccgct
     3481 ccccgctttc ggattttacg actcgtccgc cggcggacct ttttcttccc cggagccatt
     3541 ggcctcgtcg ccgccgtttc cgttgacatc gtcatccttg tcgtcgcctt ccgaggttgt
     3601 gcgggcaacc ccgacgagct tttcttccgg ttccaggtcg atcagtttga cgccctgggt
     3661 gatgcgatga ttgactggaa cttcctgaac cttcagccgg atgaccttgc cggcgttgct
     3721 gatcagcatg atgttttctt cgccctgcac ctgcaaaaca ccggaaacat tgccgatttt
     3781 tgctaccgtt ttcaggttga tcgttccttt gccgcctctg gactgcacgc ggtattcttc
     3841 tatcggcgtg cattttccaa agccgttttc cgacacggtc agcatgaacg ttccctgggc
     3901 gggaatatcc acgccgataa cgtcgtcgcc ttcatccagg ttgatgccgc ggacgccgcg
     3961 cgccgtccgt cccatatcgc gaacgtcatc ttccttgaaa cgaatcgcca tgcccagcct
     4021 ggtagccaga aaaatgtcct gatcgccgtt ggtcaactgc acatccacca gttcgtcgtc
     4081 atcatcaatg gatatggcga tgatgccccc ggcgcgcgga ttggagtagg cggccagatc
     4141 tgtttttttg ataatgcctt ttctggtcac catgacgata aatttatccg agacaaattc
     4201 cttcaccggc aaggttgcgc tgactttttc gccgggtgcc agattcacca gattaaccat
     4261 cgccttgcct ttggcggcgc gccccgcttg cggaatctgg taaactttta accaatacac
     4321 ctttcccatg ttggtgaaga ccaggaggta atgatgggtg gaggcgatga atattttttc
     4381 cacaaaatct tcttctttgg tgcccatgcc gaccttgccc cgcccgccgc ggtgctggct
     4441 cttgtagagg ctgacggcgt tgcgcttgat gtagccggta tgcgagatgg tgaccaccat
     4501 gtcttcttcg acgatcatgt cttcaatgtc gatgtcttcc gtgctggcca cgatttccgt
     4561 gcgccgctcg tcggcgtatt tttcccgaat ctcctgaagc tcttcgatga tcactttcag
     4621 gactttttgc ggatcagcca gaatgccggc cagtttggcg atcagcgccg taacgtccgc
     4681 gtattccgcg tcgatcttgt cgcgttccag tccggtcagg cgctgcaggc gcatttccag
     4741 aatggccttg gcctgaatct cggacagatg aaacgtcgcg cacaatgccg cggctgcatc
     4801 gttggggctt ttggccgcct tgatcacctt gatgatttcg tcaatgttgt tgagtgcaat
     4861 gatgtagcct tccaaaatgt gcgcacgctc ccgggcgcgc gccagatcga actgcgtgcg
     4921 gcgggtcacc acttcctggc ggaaggcgat gaatttctcc agcaccgctt taagggtaaa
     4981 aacctgcggc cgtccttcgg aaatcgccag catgatgatg ccgaaggaga cttccatttg
     5041 agtgaatttg tagagctggt tgagaatgat cgcggagctc tcgtcgcgct tgagttcaac
     5101 gaccacgcgg atgccctcac ggtccgattc gtcgcgcaga tcggagatgc cggagatttt
     5161 cttgtcgcgc accagctccg aaattttttc aatcagcgtc gccttgttga cctggtaggg
     5221 caattcggtg atgacgatgg tttcccggtc gttgcgggcg ttcttttcga tgagcgcgcg
     5281 agcgcgaatg cggataatgc ctcgcccggt tttataggcg gaaagaatcc cttcccgtcc
     5341 gttgatgaag ccggccgtgg gaaaatccgg gccgggaatg tatttcatca gctcttccac
     5401 gatcattttg ggattctgga tcagggcgat cgtcccgttg atgatttcct tcaggttgtg
     5461 gggcggaata ttggtggcca tgccgacggc gatgcccgac gttccgttga gcaagagcag
     5521 gggcaggcgc gttgccagaa tcgacggctc ggtgagcgat tcgtcgtagt tcggcacaaa
     5581 gtcgaccgtg tctttttcca gatcggccag cgcttcatcc gagatgcgcg ccaggcggct
     5641 ttccgtgtag cgcatggccg ctggcgggtc gccgtcaatc gatccgaagt tgccctgtcc
     5701 gtccacgagc gtgtagcgca tggagaagtc ctgcgccatg cgcaccagcg tgtcgtaaat
     5761 ggccgcgtcg ccgtgggggt gatatttgcc catgatgtcg ccgacgatgc gggcggattt
     5821 tttatagggc ttgttgtagc ggttgcccat ttccgacatt gcaaacagga tgcggcgatg
     5881 caccggtttg agtccgtcgc gcacgtcggg aatcgcgcgg ccgatgatga cgctcatgga
     5941 gtaatccagg taggatttct tcatttcgtc ttctatgttc accagggttt gccggtggtg
     6001 aatgtctcgt tccattttat ttcttccttc ttaaatatcc agattggata cgtacatggc
     6061 gttttggtaa atgaattcgc gcctcggctc cacttcgtcg cccatcagcg ttataaagat
     6121 ttcgtcggcg accaccgcgt cttcgacgcc gacctgcaga agggtgcgct tttcgggatt
     6181 catggtggtt tcccagagct gttcggggtt catttcgccc aagcccttgt atcgctggat
     6241 ggtcagccct ttcttgccca ggttgatgat gtggtccacc aggtccttgg cggtcgggag
     6301 aatgaccggc gcggcgcctt cttcctggcg gtcgctgtag ggcggctctc ccagcacgga
     6361 aacctgcccc atcaaattgc ggatttcacc aaactgcggc gttcggaaag tttcccggtc
     6421 aatgcaggtg gtgtggatcg ttccgttggt tttgatgtcg aaaatcatct tgtagccgcc
     6481 gtgatcggga tcttcgtcga tccggtaatc ggccacgaca ctgctgccca gggcgaccac
     6541 cgttctgccg gcgattttca gaagctcgcc ttcgttttga aacgtctcat cggtcaacgt
     6601 ggggtccccc gccgcaatcc gaaccacatt ccggtcgcga tgctcttttt caaaacggtc
     6661 cagcaggtct ttgatgcgcg tgattttttt gatgacggtc agcagtttgc ttcctgaaac
     6721 gggaaagggg cttccgtctt ccataaacag attcaccttg ttcacgccgt tttccagaat
     6781 gtaatttttc atttgttctt cgttctggat gtagaattcc ttctttttgt ccaccacctt
     6841 gtacagcggc ggctgggcga tgaacaaata ccccctctcg atcagctctc gcatctgacg
     6901 gaagaaaaaa gtcagcagca gggtgcggat gtgggatccg tcgacgtcgg catccgtcat
     6961 gataatgact ttgtgatacc gaagcttgct gacgtcgtaa tcgtcggtgc cgatgcccgt
     7021 tcccagggcc gtcacaatca ccttgatttc ctcgttctgc agcatcttgt caaagcgcgc
     7081 tttttccaca ttgagcactt tcccgcgcag cggcagaatc gcctggtttt tccggtcgcg
     7141 cccctgcttg gccgacccgc cggccgaatc tccttccact aaataaattt cactgagggc
     7201 tggatctttt tcctggcaat cggccagctt tccgggcagc gcgccgactt ccagcgcgct
     7261 tttgcggcgc gtcagttccc tggcccttcg ggccgcttca cgagcgcggg cggcttccat
     7321 gcatttgttg agaatctgcc gggcgacgga cgggttttcc tccaggtagg acccgatttt
     7381 ctcgtaaacg attccttcca ccaggccgcg gacgtcggaa tttcccagct ttgtcttggt
     7441 ctgtccttca aactgcggat ttctcacctt cacgctgatg acgcaggcca gcccttcgcg
     7501 caggtcttca ccgcgaagcg attcctttcc gtccttgatg atcttgttgg cggaggcgta
     7561 attattgaac actcgggtca gcgccgcgcg gaagccgacc atgtgcatgc cgccctccac
     7621 ggtattgatg ctgttggcga atgaaaagat gttttccaga taactgtcgt tatactgcag
     7681 agcgacttcc accgaacagt cttcccgggc gccggtcacg taaatcggct ccttgtggag
     7741 cactttccgg ttgcggttga tatattccac aaaggaaaca atgccgcctt tataaataaa
     7801 ctctgtcttc ttgtcggccc gttcatcgat cagtgcgatt ttgacgccgg agttgagaaa
     7861 agccagttcc cgaaggcggt tggcaattac atcaaagctg aattccgtct cttcaaaaat
     7921 ttcatcgtcc ggcagaaagg cgacggtggt gccgcgtccc ctgcttttgc cgaccgcatg
     7981 cagcgggccg tccggaacac cccgttgata ggtctggcga tacacaatgc cttcgcgccg
     8041 cacttccagt tcgcaggtgc gggaaagcgc gttgaccacc gacacgccga cgccgtgcag
     8101 accgccggag actttataag tttcgttgtt gaatttacct ccggcgtgca gtttggtcat
     8161 tgcgacttcc gccgccgaaa ctccctcggt tttgtgaata tccaccggga tgcccctgcc
     8221 gttgtcttcc acgacgatgc tgttgtccac ccggatttta acggtaatgg aatcacagaa
     8281 ccccgccgac gcctcgtcaa tgctgttgtc gacgacttca tacaccaggt gatgaaggcc
     8341 ttctttgctg gtggaaccga tatacatggc cggacgcttg cgaaccgctt ccagaccttc
     8401 taaaacttta atgctgtcgg catcatatgt ttctaccata accgcctttg tactccttca
     8461 acttctttga aattccatcc aaaccaattt tagattttca agggcatgac aatgcataaa
     8521 taattgtcgc cctcggccgg tttgatcagg gtcggcttca ggccgacgcc cacttccatc
     8581 accacgcgat cgatggaaat ggcggaaacg gcatcaatga cgtagttgac gttaaacccg
     8641 acttccattt cttcaccgga atacgtgatg tctatttctt ccgtggcttc cccgacgtcc
     8701 agattggtgg aattcagcgt cattttgtcc ggcgcaatcg aaataatcac accgctgtat
     8761 cgttcggaag acaccacgtt cattcttctg agcgcgtgca ggaacaattc cttgtccagc
     8821 gtcacgctta cgcctttttc cgccggaatc acccgcttgt aatcgggata gtccgcatcc
     8881 accaggttga ctttcaaaat agtgttttcc gttttaacca caaacatatt cttgtgaatg
     8941 ccgacgctga cgttttcatg aacatccgtc agttttttca cttccatgag ccctttgcgg
     9001 ggaatgatga ttcccttggc catctccgga cacggatcgg ccgtctgggc gcgggccacc
     9061 gccagccggt gcccgtccgt tgccaccatg cgccagatat gattctttcc atcggaggaa
     9121 gtttccagca gaaccccgtt taaattcttc cggctctcgt caaacgccat cgcaaaggat
     9181 gttttgctga tcaattcctt taaaaccgat cccggaatgt tgaaaaactc cacctgttgg
     9241 ctgtccgcga cgcttggaaa atcatccgcg gggagccccg gtattttgta ggtggcccgc
     9301 tggcacgtga ctttcacgat attgttggcg ctcttggtga aatggacggt gtctccctga
     9361 atttcgcgaa ccatttcaaa aagctttttc gccgacaggg taatctcacc gttttccgcg
     9421 atgtccgcct cataatcgga gatgaggctg atctcccggt ccgtggcgat gattttaatc
     9481 ttgctggctt ccgcctttaa gagaacatta tttaaaatgg gtgttgttgt ctttttttcg
     9541 acaataccta acgttttctg aatcccgtcc agaaaaatat ttctgttcac tttaaattcc
     9601 atcgccgcct cccattaata cttttatttt tttcttacct atacataacc agtattagta
     9661 ataacgcctg ttgatatgtg gatgactgtt gtttctgccc tgatttcaat cccttcaggc
     9721 cgcggcgcct gtggaagagg cgtgcagacc ctggttacgt tttgtggatg acactgtcct
     9781 cgatgtcctg aatcattttt ttaaaggagg catccgattc aatatttttt aaaattttat
     9841 tattggcgta aatcacggtg gaatgatcgc gccctccgat tttctgtccg atgtcgggaa
     9901 aggagaagtt ggtcagcttc cgggcaaggt acattgaaat ctgccgcgcg agaaccagat
     9961 tcttattctt gttgtgcgcc ttcatgtccg agatctttac attcatcttt ccggcgacaa
    10021 ccttcaaaat ctcctcgatg ctcacatcct ctttattgtt gtgcttgacg agtgttttca
    10081 ggacttcctt gaccaggtcc agatcaattt cccggttggt cagtgaagaa taggctgaaa
    10141 tccggattaa aaatccctcc agctcacgga tattggactc cacgtgcgat gcgatgtaat
    10201 gcgtcacgga cggggaaagc tccatcttgc cttcatgcat tttcttttcg atgatcgcaa
    10261 cttttgtttc gatttcggga ggctgaatgt ccgcgatcag cccccactca aagcgcgacc
    10321 gcagacggcc ttccaggttt ggaatatcct tgggaaactt atcggaggtc acgacgatct
    10381 gcttgccgga atcgtgaaga gtgttaaagg tatgaaaaaa ctcttcctgc gtcctgtcct
    10441 ttccggcgag aaactgaatg tcgtcgatca gcagacagcc gatattgcgg tatttttcgc
    10501 ggaatttctg cattcggtca tagcggatgg aattgatcat ttcgttcata aaggattccg
    10561 ccgaaacgta catgacattc aggtccggat gcgcgttgat cgtcatcaag ccgatggcgt
    10621 tgagcaggtg tgtttttccc agccctgaac ccccgtagat aaaaagagga ttataatttt
    10681 tggccggctg tttgccgacg gcaatggaag cggcatgcgc aaactggttg gaggggccga
    10741 cgacaaaccg gtcgaacgtg tagttgctgt tgaggaaaga aaagtatttg ctccgctgcg
    10801 gcgaaggcgg cggctggttt tttccctgtt cgaaaggaag ggcggcgact tccggctttt
    10861 tttccttgtc cttgctcagc ctgaaatcga tgtccacgct gatgccgatc acgctgtgca
    10921 gcgtgttttt gatcatggac agatagttgt ccatcagcca atccttgaaa aatttattgg
    10981 gcacggacaa ctggacgcac ttgtcttcca tggagacgat tttgatgggc ttgatccagg
    11041 tatcaaagtt ctgctttgaa actttttcct gaatgatttt cgtagccttg tcccagacag
    11101 attccatgat caaccttttc caaacagctt atccacagat gtggatacat ttttattgcc
    11161 tataactcat ccagcagata gcgggccagg cgattgagtt cgtccatgga ttgataggaa
    11221 atttcaatca atcctttttt ggacgaacct ttcaaacgga cgcgggccat gcacttggcc
    11281 gtcagttgct tctccagatc cgccatgaag cggtcccggg cagccgtctt tttttcagcc
    11341 ggtttttgtt tcagccggtt gacgagtctt tccgtttccc gaaccgaaag gccttttttt
    11401 aaaattacat ccagggcggc ggtctgttcc cccgccgcgt tcagcgacag aaggcagcgg
    11461 ccgtgtcccg gggtgatctt tttttccgtc agtgcggcct tcacccggtc aggcagcttc
    11521 agaaggcgga tggcgttggc aatcgtggaa cggtctttgc cgacccgggc ggagacggat
    11581 tcctgcgaaa gatgaaaggt ttccatcagc gtaacatagg cgtccgcttc ttccagcggg
    11641 ccgagctctt cacgctgaat attttcgatc agcgagagct cggcggcttc cgcgtcggtg
    11701 gctttgcgaa ccacaatcgg cacttccttg agacctgccg cctgcgccgc gcgccagcgt
    11761 cgctccccgg cgatgatttc atagccggaa tccgccctgc gcacaataat cggttgaatg
    11821 atgccgcttt gccggacgga cgcgatcagg tttttctggt cttcgtcgtt aaaatttttc
    11881 cggggctgcc agcggttcgg ccgcaattcc tcaatgccgc aggtgttgac gggcgccttt
    11941 cggcccgcgt ccgtttcgag caggtccgaa aaaatagcgc ccaggccttt gcccagggcg
    12001 tccttttttt gcgccattta cctttccccc cgaaggattt cctgagccag ctccatatag
    12061 gcaatcgcgc cgcgcgattt gatgtcatac aaaataatgg gcaatccgtg gctggggctt
    12121 tccgaaagcc tcacgttgcg cggaatgacc gtctgaaaaa cttttcggcc cagatgcttg
    12181 cgaacatctt ccgtcacgcg gtgcgaaaga gtgttgcgcg ggtcaaacat cgtgagcaga
    12241 atgccgccca gggccagatt cggattcatg ccggctttga ccagcttcac cgtattgagc
    12301 aggtgtccca ggccttccag cgcgtaatac tcgcactgca ggggaacgat cagcgtgtcc
    12361 gcggcaacga gcgcattgac cgtcatgaac cccagcgacg gcgggcagtc gatcagaatg
    12421 aattcgtagg accgctccag actttgcagc agggtgcgaa gccggctttc cctgttttcc
    12481 aggtaaacaa actcgatttc catgccgacc aggtcctgat ttgacggaac catgtccaga
    12541 ttgggaaggg ccgtctccac aatgactgat tccagaggcg cctttccaat catggcatga
    12601 taaaaattct ctttttcata cagcattcgg tcgatgccaa ggccgcttgt ggagttcccc
    12661 tgcgcgtcgg catcaatgag caaagttttc cggttggcgg cggccagcga ggcggacaaa
    12721 ttgaccgccg tcgtcgtttt gccgacgcct cctttctggt ttgcgataca tatgatttta
    12781 ctcattattt atagtatttc tctcgaaaat tgcttctggg aaaaatctgt gggaaagcta
    12841 gcacaaaaaa ggatttttta gaagggtttt ttgcctttat tttgcctttt gcaggataat
    12901 aattttgcgc ggcgggccca ggaattccaa atctatatca ttttgatata attgatatat
    12961 ttgcgacgcc tttttgtcct tgaaggccgc attcagctcg gcttcgacct cccggccctt
    13021 caaagttatc aacaggccgc ctggcgccag aaaatattct cccaatggca tcaaatcacc
    13081 cagtttcagg gaagctcggg aaatcgccac gtcaaacttt tcccgccaat tatccgagcg
    13141 gataatattc tccgtccggt cctggagcgt gaaaacaccg tccagattca acaggcgctg
    13201 gatatgcttc aggaaagaca cttttttccg gttggattcc agaaggcaaa gttgtataga
    13261 cggcatggcg attttgagcg gaaggccggg aaaaccgggg cccgtgccga tgtccacgat
    13321 gccggcgctt tcagatggga tgaagcgggc agcggtcagg gaatccagaa aatgacgggc
    13381 ggtgatttcc cgctccgtgc tcttagagat caggttgatt ctggtgttcc attggagaag
    13441 ctctttccgg tagagatcaa acagagcgag ctgacggtcg ttgagttcaa cgccgagaag
    13501 ttttgcgccc tgctgaagta agatcatatc cggattcatg ttgggcaaaa taacaaaaaa
    13561 tcaggatcac cgcaaatgga taatgtaaag cagcccaacc attacaacat attgtttgct
    13621 gaaaatattc cggaaatgcg ggggctgtgg gacggcgcag tctggcggcg ggcggaactc
    13681 ctggacgtga attgcatccg tccggaaggc agcgatcatc ggctgcggac acaatgtaag
    13741 cttttatttg atgaacaaaa cctctatgga attttccgcg tggaggatca gtacctgcga
    13801 tgtgttcaca cgtcatttca aagcgatgtc tggaaagaca gttgtgtgga attttttgtt
    13861 cagccgaaag gtgccggagg atactttaat tttgagttta actgcggcgg tgctctgttg
    13921 gcgtcttacg tgacaaatcc ggtgcgcgta aacgggcggc ttcagggatg cgtgcctctg
    13981 acgccgaatg acgacgggca gatccggcgc attgccagtc tgccttctgt tgtcgaaccg
    14041 gagattgccc agccgctggt ctggtgtctg gaattttctc ttccgtttgc tttgcttgca
    14101 aaatatgccg ggccgttcgg cgccgttaca ggacaaatct ggcgcgccaa cttttataaa
    14161 tgcgggaatg aaacctccca tccccactgg ctgtcctgga ggccgctcgc tgggcgaaac
    14221 tttcacgatc cgtcaagctt tggacaactg gtttttgatc cctaatacac ctcctcatga
    14281 ctgccggtat caatcggaat aatcttctca tgatccggca gaaataggat ggcgatgcga
    14341 tataaattct tcgtgcaatt ctttccgggc ggttttataa ggcgattatt ctctcggggg
    14401 tggcctgatc atgtcgcttg cagccaaaac gaaaaacatc agcatcgttc tgtataaacc
    14461 caaatacccc ggcaatgtcg gatcggtggc gcgggcggcc aaaaatatgg ggatcggcaa
    14521 aatcgtagtg gtcggggaaa ccggtcttga ccgggaagaa atggagcggc gatcgacgca
    14581 tctggcggcg gatgtgctgg atcaaattca atatgtcgca tccattgaag aggcgctggg
    14641 cggctttcac tatatcgtgg gcaccaccgc ccgtctgggc aaggcccgcg ggccgtttgt
    14701 ttcgccgcgc gcggcggcgc agaagattgc cgatctgtcc cggaaaaata aaatagcgct
    14761 tttattcggc ccggaagaca cggggctggc caacgatcag ctgcgcctgt gccacagcgt
    14821 ggtgacgatc ccgacctccc gcgaattcac ctcgctgaac ctttcccagg ccgcgatgat
    14881 tctctgctat gaaatattca ttgcgtcgcg tgcagcggaa accgcggcgg aggcagcgcc
    14941 gaaactggct ctgtcttccg agttggaggg catgtacgga caaatgaaag agctgctcgc
    15001 caaaatcgga tttttaaatc cggaaaatcc ggaatactgg atgctggatg tgaggcgctt
    15061 tttttcgcgc gccggactcc tgtcgcggga agtgaagatt attcgcggtg tttgccggca
    15121 gctggaatgg tatgcgtcca acaaaaaaac ttgacattgg ggctgatttg tcgtgaaagg
    15181 ggaaccctga atcatacaaa gcggccgtgc cttaaaacca aaagacggag ggggtttgac
    15241 catgaggaag attttttcat ttaccggcgc caaaaaaatc gtgtttggca gcggagcgtt
    15301 ggcggcgctg gccgcgcatg taagggaaca tcatggccaa aagccgctgg tggtcatcga
    15361 tcaaaacctg gccaagacgg ggcttaagga aaaaatatcg gcccttctgg aatccgacgg
    15421 gatcaagttt gctttttttg acaaggtgga gccggagccc cgcattgaac tggcggatga
    15481 agggg
//
LOCUS       DAOSIM010000020        15432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_3987_length_15432_cov_8.179814, whole genome shotgun sequence.
ACCESSION   DAOSIM010000020 DAOSIM010000000
VERSION     DAOSIM010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 15432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15432
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3987_length_15432_cov_8.179814"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="PLR47_01905"
     CDS             complement(<1..1399)
                     /locus_tag="PLR47_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG11718.1"
                     /translation="MNDHAGTRQALLGELASLREKIAKLERSALDQKRVKEALRESEE
                     KYRLLFEYSPLGNFYFDEKGVIVACNDHFVDIIGSSKEVLIGLDLFALRDQKIVDSIR
                     QVLEGQTVFYEDVYHSVTAKKSTPVRVIFTPVHLEGKGIIGGVAMIEDITERRRAEAA
                     LRESEEHYRSLVENADEAIIVIQDGRIRFVNARAVEAFGYSVQEFMSIPVFELVHPED
                     RDMVIRRYLEKINGDAAPTRHTYRALYKKGLVAWIEISSILINWEGRPATLNLITDIT
                     DRKKSEDALREREEKYRTILESIEDGYFEQDLYGNFIFVNDSMSRIYGYSKEELIGLN
                     YKQYTDFESGRKCFHIYLNIYKTGKPGKVVDFDIVRKDNTRRHVESSVSLIRDPTGKP
                     IAFRGIVRDVTERKQSEEALSQSFVKLRAALAATVQATAMMVETRDPYTAGHQRRVAD
                     LGRAIALEMQLTDEQI"
     gene            complement(1563..2873)
                     /locus_tag="PLR47_01910"
     CDS             complement(1563..2873)
                     /locus_tag="PLR47_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOG11719.1"
                     /translation="MRANSIQPVTPDEAVRAVRSGDHVHISATSNTPACLVEALCRRG
                     RSGELRGVRIHHLHTEGPAPYADPAFEGVFQLDSFFVGPNARRSTQQGYADYIPVFLG
                     ETQRLYREGYLPCDVALIQVSPPDERGRVSLGTSVDASVAAVEKARVVIAVINPHVPH
                     TYGDGLIHASRIHFFVEDDAPLETVHFALPDAVEAAIGKHCASLIEDGATLQMGIGAI
                     PNAVLTQLSGHKNLGIHTEMFADGVIDLVEKGVINGENKAIDRGQMVATFLLGSKRLY
                     DYTHCNDRVLMKDVGYTNDPFIIAKNPKATAINSALQVDLTGQISADSLGTKCYSGVG
                     GQIDFFYGASRSAGGKPILAMPSVTEKGISKIAPILLPGAGVVTTRAHAHWLVTEFGA
                     VNLYGRSLQERARLIIGIAHPDHREALDRAAFERFGPHYRFAPA"
     gene            complement(2984..3784)
                     /locus_tag="PLR47_01915"
     CDS             complement(2984..3784)
                     /locus_tag="PLR47_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG11720.1"
                     /translation="MPHQTVNDIKIYYEEKGTGEPLLLLDGLAFPMDLWFAQIEELSK
                     DFRVIAMDNRGIGRSDKPDDVYSMELMASDSVGLLRALGIQKAHVAGLSMGGFISQEI
                     ALSYPEMVNRLILVATGMGGHRSLTLGKPFWDRTAAAIAGKKPWEIYRIDLTLMTAPG
                     FVEQQPDTLDKAVELRMKNPQPFHAFVRQFTAAGLFDSNERVHKIDQPTLIVLGREDP
                     IFPIALAEDFRQKMPQAKMVIYENCGHAIHLEKPDRLSNDIREFLKGN"
     gene            complement(3865..5148)
                     /locus_tag="PLR47_01920"
     CDS             complement(3865..5148)
                     /locus_tag="PLR47_01920"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOG11721.1"
                     /translation="MSNAVIVSGVRTAVGAFGGSLKGVPAKDLGALVIRESLIKAGFK
                     PALPAHAREDAPDTAKNEGLCPIEQQYGKWDDRLRELAVDEVIMGNVIGAGQGQNVGR
                     QAMIRAGLPKETTAITVNKVCASGMKAIALAAATIKAGDNEVVIAGGMENMSDVPIGL
                     PKARWGHRMDMPFGKTVDLMVFDALYEIFYGYHMGAENIAARYNISREEQDKLSLESH
                     TRARNAIKSGLLKDEIVPVSIPQKKGNPIVFDTDERPMETSMEKLAKLGTAFKKDGTV
                     TAGNASGINDAAVALLVMSEEKAKALGLKPLARIKGYASGGVDPAYMGLGPVPAVRKL
                     LRITGTSIKDYGLFELNEAFASQAISCLRELGIPWDITNVNGSAISIGHPVGCSGARI
                     VLALANEMKRRNVGLGLASLCIGGGQGMAIAIEAV"
     gene            complement(5309..>6575)
                     /locus_tag="PLR47_01925"
     CDS             complement(5309..>6575)
                     /locus_tag="PLR47_01925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG11722.1"
                     /translation="DGATVVLHVGRLFNAVADHLKQKRNLGIVTHVVSDWALDLIESG
                     AISLDRSRFNGGLITASYCYGSKALYEYVHRNPIFEFYPIARLNNPFVVRRIKSLIAI
                     MNVKKIDISGESVIFHSGDNLLTGYESKLNFAVGATMTRRGKAIVALNSVDTEGNSNI
                     VIKFDGRADNTHSTLGVVKYIVTEYGVANLFGKSIRERALAMIDIAHPDHREDLLKQA
                     KALNYVYADQIYFARHAANYPALLETRKSLKDGLEIKVRPIKPSDEDKMRRLFYNFSD
                     ESKYFRYFAPKPVMPHKEMQTYVSIDYDKVLSVVAVAEKGRNERIIAEARYAYDKSAE
                     AYEVAFIVDEDFQGKGVAAFLFKYLVKIARDRGIQCFIAYVLPRNEAMLKVFEKSKLP
                     VRKSYENDSLALRFDLTQAKAEPDAAAKK"
     assembly_gap    6576..6585
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6586..7122)
                     /locus_tag="PLR47_01930"
     CDS             complement(<6586..7122)
                     /locus_tag="PLR47_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11723.1"
                     /translation="MKDSTMKGFFDYRSKVIGTDQALEKLKPGQKIFLSSGVAAPGKV
                     LTALARSDAKNLRDLEIIQLITLRKYLSHQSSDQYRLKTFNVGESISKEVAEGRVDFI
                     PANLMELPYIFLSGAVAVDVAIIQTSVPDDKGFMSLGVAVDVANIVIKQAALVIAEIN
                     PNMPVTLGETTIHISSIDY"
     gene            7269..8717
                     /locus_tag="PLR47_01935"
     CDS             7269..8717
                     /locus_tag="PLR47_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOG11724.1"
                     /translation="MKKIQKLLVANRGEIALRIIRTAKEMGLSTVAVYEKPDSEAYFL
                     RFADQAILIGDGPRRDYLNIDKIIWAARKSGADAIHPGYGFLSENPELAAACENEDIA
                     FIGPPPRVIRDLGNKVVAREIMEKASIPFVPGTGTLSEGDAGLAEAVAFGKTHGYPIM
                     FKALAGGGGRGIRKIASEEELRSQLPQARSEARSAFNDDRIYIEKCVESPRHVEVQIL
                     ADAYGHVMHLGTRDCSIQRRHQKLLEIAPADLPKNVLDAMHTTAIQAARQAGYVNAGT
                     VEFLVDPKTNDFWFMEVNTRLQVEHTVTEILTGIDIIRQQVLLAAGDRLEIPEERIHL
                     VNKAIQVRINAEDPKNNFMPEGGKRVEVYQSPGGPEIRLDGIVYQGYKIPTDYDSLLV
                     KLTVRGYSWQQTIQRLKRALKDFLIVGPKTTIPFYLSICDDADFRDGRFTTDYLDAHP
                     ELFDYPEPEREIARLSKLIAEIHSRKINPYAY"
     gene            8747..10675
                     /locus_tag="PLR47_01940"
     CDS             8747..10675
                     /locus_tag="PLR47_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HOG11725.1"
                     /translation="MKILPEERITPRELQRAGVKAVLEKMRGQKGYYLTNTERDLSQS
                     DFKNRIMPHTQILVAPERNAAGYFSLEITGGASVHVDMLRKQMNPLEKLEVLAVHMPD
                     TLFQTLCRGINLFGYRPYPENVIRLTVETFARHVHVWRVFDFLNYIPNMVPVYEEVQK
                     AGRMLEAAVCFSTGPEHTNRFYVKKVGEILDVTGPDILLAIKNHGGLGSPGRIGELVR
                     AILQKYPDLLIHYHGHNTDGNEIGRIVAAVQNGAKIVDASDHALTGFYGPPPLLTVVN
                     TLEDYGYPAVGLDKQAVIDASSKLRHERPCYRDFESQFFGFDPTVQTHKLPGGATGSS
                     FEQAQKGGFLDRMPEILQNELPRVQIELGNFWSVTPGSQILWTTAANNVVKGARYRDV
                     SDDLKNLLLERYGKFPFYKPAPEIYEAVFGKDWKKIVRREAGYQKIEDVDLDVERRIL
                     EHAIGRKATDDELVLYLQHPNDAVGFFKFEEKFGKTWVLPPKVWFRRGGFAIGDKFEF
                     PDATGKLHTIEIGPSRRTKDGSHMTHLGVDYHPEPIVTPAEEEGEKPKAALALSAKEI
                     GALARMGDIRATLKGVVNQIPVAVGDEVGAGDTLVVLEAMKMLTNIASDVDGKVSEIF
                     SSPGSSVETGDKLMLIEI"
     gene            complement(10672..11463)
                     /locus_tag="PLR47_01945"
     CDS             complement(10672..11463)
                     /locus_tag="PLR47_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOG11726.1"
                     /translation="MKIKKVANQTRIRIGLFGFGKTGRLVAEEFFQDGQFDLEWVVRK
                     THKEHHKYASRLLGYEVDDARVFSVDESTPDFYRRHPVDIIVDFSSAAAHRQYAPAAD
                     FGIRIISAISKYRAEDLEDLKRMSEKTAVLYSPNITLGINFLLVASQILQGIAPHADI
                     EIVEEHFRGKKEESGTALKIAEILGLEKERRVNSIRVGGIVGKHEIIFGLPNQTIRLV
                     HESISRAAFGQGAIFAAKWLIHAPPGLYSMENIIVDMFRKNIPVY"
     gene            complement(11540..11950)
                     /locus_tag="PLR47_01950"
     CDS             complement(11540..11950)
                     /locus_tag="PLR47_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOG11727.1"
                     /translation="MRDFKNILYAMDLDGEHFSSMVHALEFARRFTSRIHILYVNDPQ
                     AGYRHPADHEDAVALKVKEAAPEFLLENLDVVYAVSKGDTAGEIVQYARENLIDLIIV
                     GHKHRGKLYASMFDSTDVHVIDEAMLPILVFPEM"
     gene            complement(12080..12946)
                     /locus_tag="PLR47_01955"
     CDS             complement(12080..12946)
                     /locus_tag="PLR47_01955"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG11728.1"
                     /translation="MSRVSAVLLLMLSLAAMPAESGSSPDVSFSSDAMVRGGLILLSI
                     QGCESDDLQVVWMEKEIPLLRGRDDGAWRGFIAADLNQKPGVYPVAVRVPSLEMERTF
                     EIQVNDRDYGVRKIRLPRAKVDLDEASLKRVKTEARAVSALWKAECRPPEWEGSFWMP
                     VNNKIVGPFGQRSIINRQERSLHTGVDIRGKTGSPVRATNAGTVVLAADHFFTGKSVF
                     VDHGSCIFSMYFHLDQIRVEEGEQVEKGRILGSVGATGRATGPHLHWGVRISGARVNP
                     LTLIELSRDLES"
     gene            13306..13890
                     /locus_tag="PLR47_01960"
     CDS             13306..13890
                     /locus_tag="PLR47_01960"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOG11729.1"
                     /translation="MDIAAFYHSEIFKFVLLPVLIFLARICDVTLDTLRIIYVSRGMK
                     FLAPAIGFVEVLIWLVAITQIFKNLSNPICYVAYAGGFAMGNFIGIIIEEKMAIGTVV
                     IRIITQKDAEALIGCLKENRYGVTHVDAQGAMGQVKIIFTIVKRKSIDSVLEIVRGCN
                     PLAFYTIEDVRSVRKGVFPLFKAAGKGPGPAADG"
     gene            complement(13977..>15432)
                     /locus_tag="PLR47_01965"
     CDS             complement(13977..>15432)
                     /locus_tag="PLR47_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOG11730.1"
                     /translation="QDMTVLFASVREKGKSIAEIARESLGNTGFFLFISFAILMLLLL
                     TSAFLGLTAVSLTSLVPLEMMRLTPDQTIVKTVLVGGVPNAQIGGISSTSLIIITAFA
                     PVVGWLVYKRGVSVKLAVPLAVIVCIGSILYGMKYPVSLDPDVWMVILCFYTLLAAGI
                     PVWIILQPRDFTNSFLLYIGVAALFAGGIAAGFQGLTFTAPAFNLAQGADKLGPVWPF
                     LFITVACGAVSGFHALVAGGTSSKQVSRETHVKKIAFGGMLLEGLLAIGVMIAVGCGI
                     AFADFVNIAFPSAAGVKANPILAFALGVGGLLDKSLGIPPVYGTVFGILMVEGFVVTT
                     LDTAVRLNRYLFEELWQVVFRKVPKIMGSYLFNAFLCVSLMYFLAYTNAFAAIWPIFG
                     SANQLLASLALIAVSAWLVKRKKTAWFTVLPAVFMMITTIYSLVSLLVNKYLPKHNLA
                     LAVTDVALILLSIGVIVLALKSWRDLKAKAGQAA"
BASE COUNT         3324 a         4537 c         4335 g         3226 t
ORIGIN      
        1 cgatctgctc gtcggtcagt tgcatctcca aggcaatggc ccggcccaga tcggccaccc
       61 ggcgctgatg gcccgccgtg tagggatccc tggtttccac catcatggcc gttgcctgca
      121 cggtggcggc aagcgcggcg cgaagtttga caaagctttg gctcagggcc tcctctgact
      181 gcttgcgttc cgtgacgtcc cgcacaatcc cccgaaaggc gatcggtttt cccgtcggat
      241 cacggatgag agaaacggac gattcgacat gtcttctggt gttgtctttc cggacaatat
      301 caaagtcaac gactttgccg ggcttcccgg ttttgtaaat attcaaatag atgtgaaagc
      361 attttcttcc gctttcaaaa tcggtgtact gcttgtagtt caatccgatt aattcttcct
      421 tcgaataccc gtagattctg gacatggaat cgttaacgaa tataaaattg ccgtatagat
      481 cctgctcgaa gtagccgtcc tcgatgcttt ccagaatggt ccggtacttc tcctcccttt
      541 cccgcagggc gtcctccgat tttttgcggt cggtgatatc tgtgatgaga ttcaacgtcg
      601 cgggacggcc ctcccagttg atgaggatgg agctgatctc gatccacgcg acgaggcctt
      661 tcttgtagag cgcccggtag gtatgccgcg tgggggcggc atcgccgttg attttctcaa
      721 gataccgccg gatcaccatg tccctgtcct ccggatgcac cagttcaaag acagggatcg
      781 acataaattc ctgcactgaa tatccaaagg cctcgaccgc ccgggcgttg acaaacctga
      841 tcctgccgtc ctgaatcacg atgatcgctt catcggcatt ttcgaccaga gaccggtaat
      901 gctcttcgct ctcccgcagc gccgcctccg cgcgcctgcg ttcggtgatg tcttcaatca
      961 tcgccacgcc gccgataatg cccttgccct ccagatggac cggcgtgaaa attaccctaa
     1021 ccggcgtgga ctttttcgcg gtgacggaat gatacacgtc ctcataaaaa acggtctggc
     1081 cttcgagcac ctgccgaatg ctgtcaacaa tcttttgatc ccgcagcgca aacaggtcca
     1141 ggccgatcag gacttctttg gaagacccga taatgtcgac gaaatgatca ttgcacgcga
     1201 caatgacgcc cttttcatca aagtaaaaat tgcccagggg ggaatactcg aaaagcagcc
     1261 ggtatttctc ctcgctttcc cgcaacgcct ccttcacgcg tttttgatcc agcgcggagc
     1321 gctccagttt cgcaatcttt tctctcaggg aagccagttc accgagcagg gcctgcctcg
     1381 tcccggcgtg atcattcatg cggacattct cccggccggc caaaagcgcg gatggataaa
     1441 aaaggttttt gaactgcaac catgtcggcc tactccatga tggatatttc atttatgccg
     1501 ggtggatttg tagcagaaaa tggatatttg aacaagactt tgaacagatc cgatccgatt
     1561 ggtcaggcgg gcgcaaaacg gtagtggggg ccgaagcgtt caaacgcggc acgatcgaga
     1621 gcttcccggt ggtccggatg ggcgatgccg atgatcagac gcgcccgctc ctgcagggaa
     1681 cggccgtaca gattcaccgc tccgaattcc gtcaccagcc agtgcgcgtg ggcgcgcgtc
     1741 gtcaccacac ccgcgccggg aagcagaatg ggggcgattt tactgatgcc tttttccgtc
     1801 accgacggca tcgccagaat gggtttgccg cccgccgagc gcgacgcgcc gtaaaaaaag
     1861 tcgatctgcc ccccgacgcc ggagtagcat ttcgtcccca gcgagtcggc gctgatctgg
     1921 ccggtcagat caacctgcag cgcggagttg atcgccgtgg ccttcgggtt tttcgcgatg
     1981 atgaacggat cgttggtgta gccgacgtcc ttcatcagca cgcggtcgtt gcaatgcgtg
     2041 tagtcgtaaa gtcttttgga ccccaaaaga aacgtcgcca ccatttggcc ccggtcgatg
     2101 gccttgttct ctccgttgat gacgcccttt tcaaccagat cgatcacgcc gtcggcgaac
     2161 atctccgtgt ggattccaag atttttatgc ccggaaagct gcgtgagcac cgcgttggga
     2221 atcgcgccga tgcccatctg cagcgtcgct ccgtcttcga tgagcgaagc gcagtgtttg
     2281 ccgatggccg cctcgacagc gtccggcaaa gcgaagtgaa ccgtttccag cggcgcgtcg
     2341 tcttccacga aaaaatggat gcgggaggcg tgaatgaggc cgtcgccgta cgtgtggggc
     2401 acatgcggat tgatcacggc gatcacgacc ctggcctttt cgacggcggc cacggacgcg
     2461 tccaccgatg tccccaggga gacgcgcccc cgttcgtcgg gcggactcac ctgaatcagg
     2521 gcgacgtcgc acggaaggta tccttcgcga tagagacgct gggtttcccc cagaaacacg
     2581 gggatgtaat cggcgtatcc ctgctgcgtc gagcggcggg cattggggcc gacaaaaaag
     2641 gaatcgagct gaaagacgcc ttcaaacgcc ggatcggcgt aaggcgcggg gccttccgtg
     2701 tggagatgat gaatgcggac gccgcgaagt tcgcccgagc ggccgcgccg gcacagcgcc
     2761 tcgaccaggc acgcgggggt gttggacgtc gcgctgatat gaacgtgatc gccggagcgg
     2821 acagccctga cggcttcgtc aggcgtgacg ggttgaatgg aatttgctct catggacacc
     2881 cattttttat gggttgctat ggccccaacg cccttccgat cgtcaaggac acggaaaaag
     2941 actgaagact gaaggccgaa ggtatatttt gcaggccttc ggtctaattt cctttcaaaa
     3001 attctcttat atcgtttgaa agccggtccg gcttttccag atggatcgcg tggccgcagt
     3061 tctcgtaaat taccatcttt gcttgcggca tcttctggcg gaaatcttcc gccagggcaa
     3121 ttggaaaaat cgggtcctcc cggcccagaa cgatcagcgt gggctgatcg attttatgca
     3181 cccgctcgtt gctgtcaaac agaccggcgg ccgtaaactg cctcacaaag gcatggaacg
     3241 gctgcggatt tttcatgcgc agctcaacgg ccttgtccag ggtatccggt tgctgttcca
     3301 caaaacccgg cgcggtcatc agcgtcagat cgatgcgata aatttcccag ggcttctttc
     3361 cggcaatggc ggcggccgtc ctgtcccaga aaggctttcc cagcgtcagc gaccggtggc
     3421 cgcccatgcc ggtcgccacc agaatcagcc ggttgaccat ttccggatag gaaagcgcga
     3481 tttcctggga aatgaagccg cccatcgaga ggccggccac gtgggccttt tgaatgccca
     3541 gcgcccgaag caggcccacc gaatcagaag ccatcaattc catggaatac acatcgtccg
     3601 gcttgtcgct tcggccgatg ccccggttgt ccatggcaat aacccggaag tctttggaaa
     3661 gctcctcgat ctgcgcaaac cacaagtcca tcgggaaagc caggccgtcg aggagcaaga
     3721 gcggctctcc cgttcccttc tcttcgtaat agattttgat gtcgttgacg gtttgatgcg
     3781 gcatgaacaa ctccataaaa atatagggaa cggtctcgtg acctttaggt gattgaaacc
     3841 gttccctacg atataaaata ttgattaaac cgcttcgatg gcaatcgcca tcccctgtcc
     3901 gccgccgatg cacagggaag ccagccccag gcccacgttg cggcgcttca tctcattggc
     3961 cagcgccagc acgatgcgcg cccccgagca gccgaccgga tggccgatgg atatggccga
     4021 accgttgaca ttggtaatat cccagggaat gcccagctcg cgcaggcagg aaatggcctg
     4081 tgaagcaaag gcttcgttga gttcaaacag gccgtaatcc ttaatcgacg tgccggtaat
     4141 ccggagcagt ttgcgcacgg cgggaacggg ccccagtccc atgtacgcgg gatcgacgcc
     4201 gccggaggcg tagcccttga tgcgggccag gggttttaat cccagcgctt tggctttctc
     4261 ctcgctcata accagcagcg cgaccgcggc atcgttgatg ccggaggcgt tgccggccgt
     4321 caccgtgccg tctttcttga aggccgtgcc cagtttggcg agcttctcca tgctggtttc
     4381 catcgggcgt tcgtccgtgt cgaagacgat gggattgccc tttttctggg gaatcgacac
     4441 gggaacaatt tcatctttca aaaggccgct tttgatcgcg ttgcgcgcgc gggtgtggct
     4501 ctccaggctc agtttgtcct gctcctcgcg cgaaatgttg tagcgggcgg cgatgttttc
     4561 cgcgcccatg tgatagccgt agaagatttc atagagggcg tcaaacacca tcagatcaac
     4621 agttttgccg aacggcatat ccatgcggtg gccccagcgg gccttcggca ggccgatggg
     4681 cacgtcgctc atgttttcca tgccgcccgc gatcaccact tcgttgtcgc cggccttgat
     4741 ggtggcggcg gccagcgcga tggctttcat gccggaggcg caaaccttgt tgacggtgat
     4801 ggcggtcgtt tccttgggaa ggccggcgcg gatcatggcc tggcgtccga cgttctgccc
     4861 ctggccggcg ccgatgacgt tgcccatgat gacctcatca acggcaagct cccggagtct
     4921 gtcatcccat ttgccgtact gctgctcgat ggggcaaagg ccttcgtttt tggccgtatc
     4981 cggcgcgtcc tccctggcat gcgccggcaa agccggcttg aaaccggctt taatcaacga
     5041 ttcacgaatc accagcgctc ccaggtcttt ggcgggaacg cctttcagag agccgccgaa
     5101 ggctccaacc gcggtgcgca caccgctgac aatgactgca tttgacatga tctcgctacc
     5161 tccctcgata taattgatat agctgatttt gtttccatcg ccggggcaaa ataaataacc
     5221 cgaaatgcgc tgctaatcgg ccgcaagttg cggcgaagta ttccataatc cggtggttat
     5281 ggcaagaatg aattgcggga ggcagcgcct attttttcgc ggccgcgtcc ggctccgcct
     5341 ttgcctgcgt caggtcaaaa cgcaaagcca gcgagtcgtt ttcatacgat ttccggacgg
     5401 gcagctttga tttttcaaag accttcagca tggcttcgtt gcgcggcagc acataggcaa
     5461 taaagcattg aattccacgg tcgcgcgcga ttttgaccag atacttaaac aaaaaggcgg
     5521 ccacgccctt tccctgaaag tcctcatcga cgatgaaggc aacctcatac gcttcggcgc
     5581 ttttgtcata cgcgtaacgg gcttccgcga tgattctttc gttgcgcccc ttttccgcga
     5641 cggccaccac ggacagaacc ttgtcgtaat cgatgctcac atacgtctgc atttccttgt
     5701 ggggcatgac cggcttcggg gcaaaataac ggaagtattt cgattcgtcg gagaagttgt
     5761 aaaacagccg ccgcatcttg tcctcgtcgg aaggcttgat cggacggacc ttgatttcca
     5821 ggccgtcctt cagggatttg cgcgtttcca gcagcgccgg atagttggcc gcgtggcggg
     5881 cgaaataaat ctggtccgcg taaacataat tgagcgcctt ggcctgtttg agcaggtctt
     5941 cgcgatggtc ggggtgcgcg atgtcgatca tggccagcgc cctttcccga atggactttc
     6001 cgaaaagatt ggccacgccg tattcggtga cgatatactt tacgacgccg agcgtgctgt
     6061 gggtgttgtc ggcccggccg tcaaacttga tgacgatgtt gctgtttccc tccgtatcca
     6121 cggaattgag cgccacgatc gccttgcccc ttcgcgtcat ggtcgcgccg acggcgaagt
     6181 tcagcttgct ttcgtagccg gtcagcaggt tgtcgccgga atggaagatg accgactcgc
     6241 cggaaatatc gatctttttc acattcatga tggcgatgag gcttttgatc cggcgcacca
     6301 cgaaaggatt gttgagtctc gcgatcggat aaaattcaaa gatggggttg cggtgaacgt
     6361 attcgtacag cgccttgctg ccgtagcagt agctggcggt gatcagtccg ccgttgaacc
     6421 ggctgcggtc cagcgagatg gcgccggatt caatcaggtc cagcgcccaa tcggaaacca
     6481 catgcgttac gatgccgagg ttgcgctttt gcttcaggtg gtcggccacg gcgttgaaca
     6541 gtctgcccac gtgcagcacg acggtcgccc cgtcgnnnnn nnnnnatagt cgatgctgct
     6601 gatgtggatg gtcgtttcgc ccagcgtcac cggcatgttc ggattgattt cggcgatcac
     6661 gagcgcggcc tgcttgatga cgatattggc cacgtccacc gcgacgccca gggacatgaa
     6721 gcccttgtca tccggcacgg acgtctgaat gatcgcgacg tccaccgcca ctgcgccgct
     6781 taaaaaaatg tacggcagct ccatcaggtt ggcggggatg aaatcaaccc ggccctcggc
     6841 gacttccttg ctgatgcttt ctccgacgtt gaaggttttc agccggtact ggtcggagct
     6901 ctggtgggac aggtattttc gcagcgtaat gagctgaatg atctccagat cgcgcagatt
     6961 tttcgcatcc gaccgggcca gcgcggtgag gactttcccc ggcgccgcaa cgccgctgga
     7021 taaaaatatc ttctgcccgg gcttcagctt ttccagcgcc tgatccgtac cgatcacctt
     7081 gctgcgataa tcgaagaaac ccttcattgt actgtctttc ataaggacgc cctcaataaa
     7141 cctgcgacac gttaaaatat ttcgtcaaaa taatcaatgc aagactctgt gcttgacagg
     7201 ctttgtgcgg ggcaagtata actccatcaa atggggacgg catcgcagcg aaagaaggaa
     7261 gggcgtttat gaaaaaaatc caaaaattac tggtggccaa ccggggggaa atcgctttgc
     7321 gcattatccg caccgccaag gaaatggggc tttccaccgt cgcggtttat gaaaaaccgg
     7381 acagcgaagc gtatttcctg cgatttgccg atcaggccat tctgatcggc gacgggccgc
     7441 gcagggatta tctgaatatt gataaaatca tctgggcggc gcgaaaatcc ggcgcggacg
     7501 ccattcaccc cggatacggc tttttatcgg aaaatccgga gcttgccgcc gcctgcgaga
     7561 acgaagacat cgcgttcatc ggcccgccgc cccgggtgat ccgcgacttg ggcaacaagg
     7621 tcgtcgcccg cgaaatcatg gaaaaggcct ccattccctt tgttccggga accggcactc
     7681 tttccgaagg cgatgcgggc cttgcggagg ccgtggcctt cggcaaaacg cacggctatc
     7741 cgatcatgtt caaggcgctg gccggcggcg gcggtcgcgg catccgtaaa atcgcaagcg
     7801 aggaagagct gcgctcccag cttccgcagg cgcgctccga ggcgcgctcc gccttcaacg
     7861 atgaccgcat ttacatcgaa aaatgcgtcg agtcgccgcg ccacgtcgaa gtgcagattc
     7921 tggccgatgc ctacggccac gtgatgcatc tgggcacgcg cgactgctcc attcagcgcc
     7981 gccaccagaa gctgctggaa atcgcgccgg ccgacctgcc gaaaaacgtg ctggacgcca
     8041 tgcacacgac cgccatccag gcggcccgcc aggcggggta tgtcaacgcc ggcaccgtgg
     8101 aatttctggt cgatccgaaa acgaacgact tctggttcat ggaggtcaac acccgcctgc
     8161 aggtggagca taccgtgacg gagatcctca ccggcattga catcatccgc cagcaagtgc
     8221 tgctggccgc aggcgaccgc ctggaaattc ccgaagagcg cattcatctg gtcaacaagg
     8281 ccatccaggt ccgcatcaac gcggaggatc cgaaaaacaa cttcatgccc gaaggcggca
     8341 agcgcgtcga agtgtatcaa tcgcccggcg gaccggaaat ccggctcgac ggcatcgtct
     8401 atcagggcta caaaattccc accgattacg attcgctcct ggtcaagctc accgtgcgcg
     8461 gctattcctg gcagcagacg attcagcggc tcaagcgcgc cctcaaggac ttcctgattg
     8521 tcggccccaa aaccacgatt cccttttacc tgtccatctg cgacgatgcg gatttccggg
     8581 acggccgctt tacgaccgac tacctcgatg cccatcccga actgttcgat tatccggagc
     8641 cggagcggga aatcgccagg ctgtccaaac tgatcgcgga aatccactcg cggaaaatca
     8701 atccctacgc ctactgaacg ggcaaagctg caaggagacc tatcgcatga aaatcctccc
     8761 cgaagaaaga atcacccccc gcgaacttca gcgcgccggg gtcaaagccg tcctggagaa
     8821 aatgcgcgga caaaaagggt actatctcac caataccgag agggatcttt cgcagtcgga
     8881 tttcaaaaac cggatcatgc cccacaccca gatcctcgtc gccccggagc gcaacgcggc
     8941 cggctacttt tcgctggaaa tcacgggcgg agcttcggtc catgtcgata tgctgcgcaa
     9001 acagatgaat ccgctggaga aactcgaggt ccttgccgtc cacatgccgg atacgctttt
     9061 ccaaaccctc tgccgcggca tcaacctgtt cggctaccgc ccgtatccgg aaaacgtcat
     9121 ccgcctcacc gtggaaacct ttgcccgcca tgtccacgtc tggcgggtgt ttgacttttt
     9181 aaattacatc cccaacatgg tccccgttta cgaagaagtc caaaaagcgg ggcgcatgct
     9241 cgaggcggcc gtctgctttt cgacggggcc ggagcatacc aaccggttct acgtcaaaaa
     9301 agtgggcgaa attctggacg tgaccggacc ggatattctg ctggccatca aaaaccacgg
     9361 cggccttgga tcgcccgggc gcatcggcga actggtgcgc gccatcctgc agaagtatcc
     9421 cgacctgctg attcactacc acggacacaa caccgacggc aacgaaatcg gccgcatcgt
     9481 ggccgccgtg cagaacggag caaaaatcgt ggacgcgagc gaccacgcgc tgaccggctt
     9541 ctacggaccc ccgccgctcc tgacggtggt caacacgctg gaagattacg gctatcccgc
     9601 cgtcgggctg gacaaacagg ccgtcatcga cgcgtccagc aaactgcggc acgaacgccc
     9661 ctgctaccgc gatttcgaat cgcagttttt cggatttgac ccgacggtgc agacccacaa
     9721 actgcccggc ggcgcgacgg ggtccagctt cgaacaggcg caaaagggcg gcttcctgga
     9781 ccgcatgccg gaaattctgc agaacgagct gccccgcgtg caaatcgagc tgggcaactt
     9841 ctggagcgtc acccccggtt cgcagatcct gtggaccacg gccgccaaca atgtcgtgaa
     9901 gggggcgcgc taccgggacg tgagcgatga tttgaaaaat ctgctgctgg agcgctacgg
     9961 caagtttccg ttttacaagc cggcgccgga gatctacgaa gcggtcttcg gaaaggactg
    10021 gaaaaaaatc gtaaggcgcg aagccggcta ccagaaaatc gaagacgtcg atctggacgt
    10081 ggaaagaaga atcctggagc acgcgatcgg ccggaaggcc accgacgacg aactggtgct
    10141 gtatctgcag catcccaacg acgcggtggg cttcttcaaa ttcgaggaaa aattcggcaa
    10201 aacatgggtg ctgccgccca aagtgtggtt ccggcgcggc ggtttcgcca tcggcgacaa
    10261 gttcgagttc cccgacgcca ccggcaaact gcacaccatt gaaatcggcc cttcccgccg
    10321 caccaaagac ggctcgcaca tgacgcatct gggcgttgat taccaccccg aaccgatcgt
    10381 cacgccggcc gaagaggaag gagaaaagcc taaggccgcc ttggcgcttt ccgccaagga
    10441 aatcggggcg ctggccagaa tgggcgacat tcgcgcaacc ctgaagggcg tcgtcaacca
    10501 gatcccggtg gcggtgggcg acgaggtcgg cgccggcgac acgctggtcg ttctggaagc
    10561 catgaaaatg ctcaccaaca tcgcatcgga cgtggacggc aaggtctcgg aaattttttc
    10621 gtctcccggt tcatccgtgg agaccggcga caaactgatg ctgattgaaa tctagtaaac
    10681 gggaatgttt ttccggaaca tgtccacgat gatgttttcc atggaataaa ggcccggcgg
    10741 agcgtggatc agccatttgg cggcaaaaat ggcgccctgg ccgaatgccg cccggctgat
    10801 gctctcatgc accagacgga tggtctggtt gggcagtccg aaaatgattt cgtgcttgcc
    10861 cacaattcct cccacgcgaa tggaattgac gcgcctttcc ttttcgaggc ccagaatctc
    10921 cgcgattttc aaagcggtgc cggattcctc ctttttgccg cgaaagtgct cttccacaat
    10981 ttcaatatcc gcgtgcggag cgatcccctg aaggatctgc gacgccacca gcagaaaatt
    11041 gatgcccagg gtgatgttcg gcgaatacag aaccgccgtc ttttcggaca tccgctttaa
    11101 atcctccagg tcttccgccc tgtacttcga gatggcggaa atgatcctga tcccaaaatc
    11161 ggcggcggga gcgtactgcc ggtgcgcggc ggcgcttgaa aaatcaacga tgatgtccac
    11221 cgggtggcgc cggtaaaaat caggggtcga ttcatccact gaaaaaaccc tcgcgtcatc
    11281 cacttcatac cccagcaggc gggatgcgta tttgtgatgc tccttgtggg ttttccggac
    11341 cacccactcc aggtcgaact ggccgtcctg aaaaaattct tccgccacca ggcggccggt
    11401 tttgccgaag ccgaaaagac cgatcctgat ccttgtttga ttcgcgactt ttttgatttt
    11461 catgggcaaa atcctcctct gcgcacgcgg gcagcccccc aaggctgccg ctcataagga
    11521 atcttccctg ggagaagccc tacatttccg gaaagactaa gatgggcagc atggcctcat
    11581 cgatgacgtg cacatccgtg ctgtcaaaca tcgatgcata cagcttgccg cggtgtttat
    11641 gcccgacaat aatcaggtcg attagatttt ccctggcgta ttggacgatt tctccggcgg
    11701 tgtcgccttt ggagacggcg taaacgacat ccagattttc cagcaggaat tcgggcgctg
    11761 cttccttgac tttcaaggcc acggcatctt catggtccgc cggatggcgg tagccggcct
    11821 gcggatcatt gacatagagg atatgaatcc ggcttgtgaa acgccgcgca aattccagcg
    11881 cgtgcaccat ggaagaaaaa tgctccccgt ccagatccat cgcatacaga atatttttaa
    11941 aatcgcgcat tcttaccctc cgaaccgccg gaattatata ccatcagcaa aggaagtcaa
    12001 ccttttgggg cacttttgac ggccggagcc ggtgttccga aggacgtcat ctgcagcgcg
    12061 cccgggcgct tccgcccgtt tatgactcca ggtcccggct caattcgatc agcgtcaaag
    12121 gatttaccct ggccccgctg atccgcacgc cccagtgcag atgcgggccg gtcgcgcgcc
    12181 ccgtggcgcc gacgcttccg aggatccgcc ccttttcgac ttgctctccc tcttcaaccc
    12241 gaatctgatc cagatggaaa tacatgctga agatgcagct tccatggtcc acaaaaacgg
    12301 atttgcccgt aaagaagtga tcggcggcca gaacaaccgt gccggcattg gttgcccgaa
    12361 ccggactgcc tgttttgccc cggatatcca cgcccgtgtg caaagagcgc tcctgacggt
    12421 tgatgatgct tctctgtccg aaaggcccga cgattttgtt gttcaccggc atccaaaagg
    12481 aaccttccca ttccggaggg cggcattcgg ccttccacag agcgctgacg gcccgcgctt
    12541 ccgtctttac tcttttcagg gaggcttcgt ccagatcgac tttggcgcgg ggcagccgga
    12601 ttttgcgcac gccgtaatcc cggtcgttga cctgtatttc aaaagttctt tccatctcca
    12661 gagaaggaac gcgcacggcc accggataaa caccgggctt ctgattcaga tcggcggcga
    12721 taaacccccg ccacgcaccg tcgtcccgac cacggagaag gggaatttcc ttttccatcc
    12781 ataccacctg aagatcgtcg gattcacagc cctggatgct gagcaggatc aggccgccgc
    12841 gaaccattgc gtcggacgaa aaagacacgt ccggcgaaga cccggattcg gccggcatcg
    12901 ctgccaggct cagcatcaac agcagcacag cggatacccg actcatgaaa gccgccttgc
    12961 ggcccttcgg tctcgctggc gcgcgaccct cggcaaacgg cagaatcccg cgtcttttcg
    13021 aggcagccgc tccgtttgcc ggcgcgggct tcgaaatctt ccagcccgca gtcggcgtgg
    13081 aagcggcgga aaacccgccg ggggaagggt aaaccggcga atgcggtgag gcagcgtgtc
    13141 gggccatgag attctcctta aaggcgttgt ccacgtcagt gtacgcagga tttgtttttt
    13201 tgcaaccctg aaatggcccg tgtcttgaaa aaatgaaagc ggattgactt gcggcgccgt
    13261 atcgcctata gtgtttgcgc cttcttgaag gaatggagag cttcagtgga tattgccgct
    13321 ttttatcatt cggaaatctt caaatttgtc ctgcttcctg tcttgatttt tcttgcgcgg
    13381 atttgcgacg tcacgctcga tacgctgcgc atcatctatg tgtcgcgggg aatgaaattt
    13441 ctggcgccgg cgatcggctt tgtcgaagtg ctgatctggc tggtcgcgat cacgcagatt
    13501 ttcaagaacc tgtccaaccc gatatgctat gtggcctatg cgggcggttt cgcgatgggc
    13561 aactttatcg gcatcatcat cgaagaaaaa atggcgatcg gcaccgtggt catccgaatc
    13621 atcacgcaaa aggacgcgga ggcgctcatc ggctgcctta aagaaaaccg ttacggcgtc
    13681 acgcacgtgg acgctcaggg ggccatgggg caggtaaaga ttatttttac cattgtcaaa
    13741 agaaagtcca tcgattccgt gctggaaatc gttcgcggct gcaacccgct ggcgttttat
    13801 accattgaag acgttcgttc cgtccgcaag ggggtcttcc ccctcttcaa agccgccggc
    13861 aagggacccg ggccggccgc ggacggatag gcggaccgga gaagtcccgc caactgccgg
    13921 gaaaactccc gtcgccgaat agccggtcgg ggagtcaccc ttattccccg tttccgttat
    13981 gccgcctgcc ccgcttttgc ctttaaatcc cgccagctct tgagcgccag cacaatcacg
    14041 ccgatggaaa ggaggatcag cgcgacgtcc gtcacggcca gcgcgaggtt gtgcttggga
    14101 aggtatttat tgaccagcag cgacaccagc gaataaatcg tggtgatcat catgaaaacc
    14161 gccggcagca cggtgaacca ggccgttttc tttcttttga ccagccaggc ggaaacggca
    14221 atcagcgcca gcgaagccag aagctggttg gcggagccga aaatcggcca gatggccgca
    14281 aacgcgttgg tgtaggccag aaagtacatc agcgatacgc acagaaaggc gttgaaaaga
    14341 taagacccca tgattttcgg aactttccgg aaaacgacct gccagagctc ctcaaacaga
    14401 taccggttca agcggacggc cgtgtccagc gtcgtgacga caaacccttc caccatcaga
    14461 attccaaaga ccgttccgta cacgggcgga atgccgagcg atttgtccag aaggccgccg
    14521 accccgaggg caaacgccag tatgggattg gccttgacgc cggcggcgga tggaaaggcg
    14581 atgttgacga aatcggcaaa agcgatcccg cagccgacgg caatcatgac gccgatggcg
    14641 agcaggcctt cgagcagcat gccgccgaaa gcaatctttt tgacgtgcgt ttccctggaa
    14701 acctgtttgc tggacgttcc tccggccacc agcgcatgaa aaccggaaac cgcgccgcag
    14761 gctacggtaa tgaacagaaa aggccagacc ggccccaact tgtctgctcc ctgcgccaga
    14821 ttgaaggcgg gcgccgtaaa ggtcaggcct tggaagccgg cggcgatgcc tccggcaaaa
    14881 agcgcggcca ctccgatata cagcaaaaag gaattggtga aatcgcgcgg ctgcagaatg
    14941 atccagacgg gaattcccgc cgcgagcagc gtataaaagc agagaataac catccagaca
    15001 tccgggtcca gggaaacagg atatttcatg ccgtacaaaa tggagcctat gcagacgatg
    15061 acggccaggg gtacggccag cttgacgctg acgcctcttt tgtacaccag ccagccgacg
    15121 accggcgcaa aagcggtgat gataataagg gacgtcgagg agatgccgcc gatttgcgcg
    15181 ttgggtacgc cacccaccag cacggttttg acaatcgtct ggtcgggcgt gagcctcatc
    15241 atctccagcg gcaccagcga cgtcagggaa acggccgtca gccccagaaa ggcggaggtc
    15301 agaagcaaaa gcatcaggat ggcaaagctg atgaacagga agaagcctgt gttgcccagg
    15361 gactctctgg cgatctcggc gatggacttg cccttttccc gaacgctggc aaacagcacg
    15421 gtcatgtcct gg
//
LOCUS       DAOSIM010000021        15250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_4023_length_15250_cov_6.370253, whole genome shotgun sequence.
ACCESSION   DAOSIM010000021 DAOSIM010000000
VERSION     DAOSIM010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 15250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15250
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4023_length_15250_cov_6.370253"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..400)
                     /locus_tag="PLR47_01970"
     CDS             complement(2..400)
                     /locus_tag="PLR47_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOG11731.1"
                     /translation="MVKVTEEIKESLKGTKIAFLATASKDGAPNVVPIGAFKFLDNET
                     LLISDQFFNKTLKNLQENPRISLVWWGEKGGFQVKADITIHTEGEVFRQNVEWVRGIK
                     ETLRPKSAVVGKITDVYLIKSGPDAGKKIL"
     gene            complement(549..1442)
                     /locus_tag="PLR47_01975"
     CDS             complement(549..1442)
                     /locus_tag="PLR47_01975"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HOG11732.1"
                     /translation="MNESLIFQETPDLVNPYLFIGYRGWLNAGELGTGSIEYLRRKLN
                     ARKFAAIDPGHFYIGQVPGFDSAQIMRPQTVVEGGLIKSLEEPANEFFYWQSGGENDL
                     ILFTGFEPNVAWPEYTSAILSMAKRCNVCRIYTLGGVFDQVPHTRETSVYAVLSRPEM
                     KREFQSFPLLNYNGPCSFSTLLVNHAGRAGIEAASIVARVPPYIQTFHEKIACDLLQK
                     VFAHTSLHVDLSDLRKIGNTVAELMDNDFRQNKTAMEQLRKLEEMYDAALIQGYGYKE
                     VNVDELMQEMLDTKMSGKKPH"
     gene            1967..3157
                     /locus_tag="PLR47_01980"
     CDS             1967..3157
                     /locus_tag="PLR47_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOG11733.1"
                     /translation="MEYRFELPTKMIIGEGCSKQLGENVKLTGAKHVLCVFDKGVEGA
                     GLVAPLIADMETAGLKVTPFNGVLPDPPMEVIDQCTQLAREAKPDVFVAVGGGSSIDT
                     AKAVNANLSNPGTLRDHAINLNGLKVFPFENPLKPFFALPTTAGTGSEVSPSAVVTDH
                     EAKLKVSVMAPDLVPTMAIIDPALTVGLPVNVTASTGLDAFAHAMEGLMGGLAIFTPS
                     PMRDSFAFTAIELVLESLLPAMKNGKDIKARTDMSYAAFMSILGAGGGLSMGHCLGHA
                     MGEVCGIHNHGFLCASILPYNIEFMAELIPQKLNKLASLFKIDPNGKSVAQIGTAIKL
                     AIQAFYKDCGIPPLKDMGLNLSDTEEIIHHTTSGTWYLLASKKPSEEELTRWIQAVYE
                     GGNA"
     gene            3154..4581
                     /locus_tag="PLR47_01985"
     CDS             3154..4581
                     /locus_tag="PLR47_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOG11734.1"
                     /translation="MKTYDKIYINGQWVSPAGKDKVKVINPCTEEAIASVPLGNEEDV
                     QKAVIAARQAFPSWSKTSVADRIKLISKLSEAIAARQNEIGDTISREMGMPSAWSRMI
                     QVGLPVATLNSFVPILENYSFSYPMGRTEIVKEPIGVCGFITPWNYPLHQLVGKVAPA
                     LAAGCTMVAKPSQLAPLNAYAFTEIMQEIGVPPGVFNLVPGAGSKVGYALSSHPDVDM
                     VSLTGSTKSGIAVAQAAATTIKRVTLELGGKSPNVLLEDADIPMAVTKGVYNCFLNSG
                     QTCIALSRMIVPAHKQKEIVEMAKAIVESMVMGDAFTDGVYLGPMVDDAQRQSVREYI
                     RAGIREGATLVTGGPETPENLPKGFFVKPTLFADVKPDMIIAQEEIFGPVLCIIPYRT
                     EEEAIAIANNSVYGLSGSVWSADSEKARAAARQIRSGQVFINGADFDIDAPAGGYKQS
                     GLGRERSKYGLEEYLEIKALIAQNP"
     gene            4661..5596
                     /locus_tag="PLR47_01990"
     CDS             4661..5596
                     /locus_tag="PLR47_01990"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOG11735.1"
                     /translation="MNKVMTMQKAIAEHVHDGDFLYIGGYICRTPFSAVHELIRQGKK
                     ELTITRGNASDDFDMLIGAGAVKRFISTFISLGVYGLARCYRRSLEKGIPHKIEVEEY
                     TNLSLPMMLMAGAMGMPFIPVKDMVGTDLMNVKGFMGEDKYRMISSPFDGSPVLLVPA
                     LKPDVGIIHVQQADEEGNAQMWGIGGDCKYGANASKKVIVSCERIVSRDVIGKDPSRT
                     IVPDFKVVAVVEEPFASHPGYTPGFYDLDFSFGYLYQQASNTEEGFRAFLQEWIMDIP
                     DRASYIQHYIDRFGYAQFRKLQAKLDYGFPVSYAY"
     gene            5611..6411
                     /locus_tag="PLR47_01995"
     CDS             5611..6411
                     /locus_tag="PLR47_01995"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOG11736.1"
                     /translation="MIEHPKDYIKPELMACCGAREIHDGDVVVVGTGFPAMSANIAKH
                     THAPGAVMMQESGVYDARPGRPALSVGDACLNPGSAMIGGLTDVMGMFLQGGWVDVGF
                     LSGAQVDRYGNLNTTCIGPYDSPRSRLPGSGGANPIGALARRTLIIVQHDPRRLAEKV
                     DFITTPGYLNGPGARERWGLPAATGPWVIITNKGVMRFDAETKEAYLESYHPGCTLEE
                     IRKNTGWDLKISAEVHETEPPRAVELKAIREVLDPHRMIRVYEGKGYV"
     gene            6476..7642
                     /locus_tag="PLR47_02000"
     CDS             6476..7642
                     /locus_tag="PLR47_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG11737.1"
                     /translation="MDFSLNEEQRMLIRTLQTMGEREKLKERAAQIDRSGEFPVDLLE
                     KYGEMGLLGMTLSPDYGGGGQPALHALLAIEEMAKYSPIIAGPIFESNVGPVRVIDLF
                     GTEEQRKTIVPGVCKGELSVSVCMTEPEAGSDLTSLSTKAVDDGDHYILNGRKVFITG
                     GGHASHYFVYTRFGDAPGYKGIGGLLVEKGTQGFTFGKQEDFLGLRGMPSCELIFEDV
                     RVPKKNLVIKAGDFNKLMLTFDIERCGNAAMCLGVAGAALNESIAYAQARQAFGRPIC
                     EFQAIQFMTVDMAMKLDAARLLVYRAAAGAGQGLPSIYEASMAKCFANEMVIEVTNTA
                     MQVFGGYGYSKEFPVERMLRDARAWGVAGGTVQMLRLTMASVLYGRRFDQRRGK"
     gene            complement(7724..8188)
                     /locus_tag="PLR47_02005"
     CDS             complement(7724..8188)
                     /locus_tag="PLR47_02005"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8051..8053),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOG11738.1"
                     /translation="MASPVQKAADFELPDQDGKMWKLSDAVKEYRAVVLAFYPKDDTG
                     VUIRQFVEFQQNIKKYEAGKVKILGLSRDSAESHRKFIAKHGLHNMTLLVDEKGKIAK
                     RYDADHWILPLSKRVYLIVDQNLNVLYRKDMGFGLLPEQTQTLMMEIDRLIK"
     gene            complement(8394..9698)
                     /locus_tag="PLR47_02010"
     CDS             complement(8394..9698)
                     /locus_tag="PLR47_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11739.1"
                     /translation="MKRFGLIFLALLVSAMPSATKAEENYTFDLSEIEKKPYHFSGYI
                     EAKPVLYGLNSNSAFYQLRFFDDPNRQALPEANGRVRLEGSYEKSIFRVFARLNTDLQ
                     YNDYAHGSEQTTFYEAHASAKPMPAVTIDVGKKTLKWGKGYAWNPVAFADRPKDPDDP
                     EQAMEGYIVATADLIRSFDGPLTIVSFSPVLFPVYDHINSNFGQNNKLNTAGRLYFLF
                     YDTDIDVMFMTGGSRPDRYGFDFSRNITTNLEIHGEYAWIQNTQKTVLDATGAAKAVE
                     GQAHTFLIGLRYLTSFDLTAITEFYHNSAGFDRDDMQNFYSLVSRSFDAYQTTGDQTG
                     LERAAQLADSGYARPNAMRNYLYIRLSQKEPFDILYFTPALTCMINTDDSSANVMPEF
                     LYTGVTNLELRFRAGVMIGGQNTEFGEKQNDYRIELRAGYYF"
     gene            complement(9699..10433)
                     /locus_tag="PLR47_02015"
     CDS             complement(9699..10433)
                     /locus_tag="PLR47_02015"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOG11740.1"
                     /translation="MKKTIYLVFALIIMLPFSAFAVDGTKLLQEIDRNLSPESYESYR
                     KLINIEPDGKKKEFTLFTVKKGLDKVASVFLAPASEKGRSTLRLGDNMWLHIPNVGKP
                     IRITSLQSVVGGVFNNADILQLDYAAEYDVLAVKEEGGEYLLLLKAKSKSVAYDQLKI
                     RADKAKRLPAKIECLTETGMLIKTLYFKNVKDFGGGIVRPAVIETDSPLYKGYKSVMI
                     MVQIKKRMLKDEVFSLTFMPNLESLR"
     gene            complement(10448..11686)
                     /locus_tag="PLR47_02020"
     CDS             complement(10448..11686)
                     /locus_tag="PLR47_02020"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG11741.1"
                     /translation="MRNLFKIAIRNLIRYKRRTLLTASLITIGVVFVLVFVSVSGSFK
                     SMITGQITDSMMGHLQIHRKGYVASIDNLPLNLNMKPNAYQTIRQALAADEQVEAFSP
                     RLKFGAMFSNFTETTNIRLNGIDPEAEFRTVPLLPSRILEGKSTVGRGEILIPALLAK
                     GMKVKPGDPVVIVATNADGSVNGMQFTVAGILESATGPGGRDGYVRMEDAAQVLRMTE
                     PEISEVAIRLKDFGRLAAAVQHLKNATAGEVNPQGQPLFEVHSWEDLSPFFNIARMID
                     IMTFFIRIMLIAIVLISIMNVMVMAVYERIREIGTIAAIGTPPGKIRSMFILEGFCMG
                     VVGAGAGVGIGAVLIALLNLIGIRFDFGQQKGLLLKATLDPPDLLVVSVIVITVAVLA
                     SLQPAVKASRMEPIDALRHV"
     gene            complement(11679..12383)
                     /locus_tag="PLR47_02025"
     CDS             complement(11679..12383)
                     /locus_tag="PLR47_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG11742.1"
                     /translation="MALVTAAGITKDYRTEELTVPALRNLNFEIEPASFVSFVGPSGS
                     GKTTLLNLIGCLDKPTAGRLTVAGTDVGTLGRKESAAFRGNHLGFIFQDFNMIPVLTV
                     YENIEYPLIMVQNIPFRERKERISRMLAAVGMDDQGGKYPHQISGGQKQRVAIARALV
                     TNPALVLADEPTANLDSQTSYAVIGLMKKMRDDLGTTFIFSTHDTKIVGEAEVLFRLV
                     DGRLAEKELKEGTAHA"
     gene            complement(12462..13187)
                     /gene="prxU"
                     /locus_tag="PLR47_02030"
     CDS             complement(12462..13187)
                     /gene="prxU"
                     /locus_tag="PLR47_02030"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083760882.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(12933..12935),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HOG11743.1"
                     /translation="MAEEIKAGCARPTGGPVGEKPAAKKGTTEEPVIKEVKSMVTVGK
                     KAPDFTAPAYQKGKFINVKLSDYLGKWVVLCFYPGDFTFVUATEISAVAEKYPEFQKL
                     GVEVLSMSVDSVFVHKMWNDHELSKMVKGGIPFPMLSDGGGKVGDVFGVYLQDAGVEA
                     RGRFIIDPDGIIQGFEVLTPPVGRNVMESIRQIQAFQHVRKTKGAEATPSGWKPGKAT
                     LKPGPDLVGKVWEVWKTKMAFDD"
     gene            complement(13216..14916)
                     /locus_tag="PLR47_02035"
     CDS             complement(13216..14916)
                     /locus_tag="PLR47_02035"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG11744.1"
                     /translation="MANRKRIIVIGGSAAGPKAAAKARRIDNDAEVIILQKDADLSMA
                     SCGYPYYVGGFFNDRNQLLCTPTGVVRNPIFYLNAKDIDAEVNTEVTGIDTKNHSITF
                     KNIVTGKTGELTYDKLVIATGAVPRMPPVPGVELDGITTLQSMKDADFLRKIRDEGTI
                     KKAVVIGGGLIGIETCEALQLAGIEITVIELLPQLLTFLDWELAKIVENHVKSKGANV
                     ITENGIAAFLGEDGKLTAVKLQNGTELPCELAVVAIGVRPNVKLAKEAGLKIGELGGI
                     DVNEYMQTSDPDIYAVGDCVETLNRITGKKVHAPYGDLANLQGRVAGENAASANCVTF
                     PGTIQTGICKVFDYAAGSTGLSETVAKRLGYSDMITVMNASPDKPGFMEGKLLLTKLI
                     AERKSGRILGAQCIGPGNVAKQIAQWAMAIQGKLTVEDLVNADLPYAPPFSLAIDHFV
                     ATAHLMQNKMKGRLKGISCKEVHDKLKAGEKPFIIDTRGPEEFEEMRLGIGETLIPLG
                     AIRKRLQELPKDKNKEIICYCKISLRGYEAAIALEGNGWKNVKVMEGGIMAWPYPREK
                     "
     gene            complement(15045..>15250)
                     /locus_tag="PLR47_02040"
     CDS             complement(15045..>15250)
                     /locus_tag="PLR47_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11745.1"
                     /translation="LCVSLCPVGSIDADDESIITGICIKCCACIKNCPVEAKYFDDEN
                     YLWHKQELEVAFTSPRREPEWFL"
BASE COUNT         3390 a         3986 c         4189 g         3685 t
ORIGIN      
        1 cttacagtat ctttttccct gcgtcgggac cgcttttgat aagataaaca tccgtaattt
       61 ttcccacgac agcggacttg ggacggagcg tctctttgat gccgcggacc cattcgacat
      121 tctgccgaaa gacctctcct tccgtgtgga tcgtgatgtc ggccttgacc tgaaagccgc
      181 ctttttctcc ccaccagaca agggagattc tcggattctc ctgaaggttc ttcagggttt
      241 tgttgaagaa ctgatcggag atgagcagcg tttcattatc cagaaacttg aacgcgccga
      301 tggggacgac attgggggct ccgtcctttg acgcagttgc aagaaacgcg attttcgttc
      361 ctttcaggct ttccttgatt tcctctgtta ctttaaccat ggcaatatcc tccggcattg
      421 gtttacgttg cttcgtcgat caacggaagc gcttgtttcc gggaaacatc ttcgaaaaaa
      481 tcaaaagcgc cttccgaagg cttgccggtc gttgaacagg cgaagccccg cgggatatca
      541 aaagttcatc agtgcggctt tttgccgctc attttcgtat ccagcatttc ctgcatcagc
      601 tcatcgacgt tgacttcctt gtaaccataa ccctgaataa gcgccgcgtc atacatttct
      661 tccagcttcc gtaattgttc catagcggtt ttattttgcc ggaagtcatt gtccatcagc
      721 tcagccacgg tatttccgat ttttctcaaa tcgcttaaat cgacatgaag ggatgtgtgg
      781 gcgaacactt tctgcagaag gtcgcaggct attttttcat gaaaggtctg aatatacggc
      841 gggactctgg ccacgatgct ggccgcctca attcccgccc ggccggcatg attcacaagc
      901 agcgtggaga agctgcacgg tccgttgtag ttcaggagag gaaacgattg aaactcccgc
      961 ttcatctcgg gacggctcag cacggcatag acgcttgttt cccgcgtgtg gggaacctgg
     1021 tcgaaaacgc cgcccagcgt atagatgcgg caaacgttgc agcgtttggc catgctcaaa
     1081 atagccgacg tgtactccgg ccaggcaacg ttcggctcaa aacctgtaaa aaggatcagg
     1141 tcgttttcgc cgccgctttg ccagtaaaaa aattcattgg ccggttcttc gagggacttg
     1201 atcaggcctc cttcaacgac ggtctggggt ctcatgatct gcgccgaatc aaagcccggg
     1261 acctgtccga tatagaaatg cccgggatcg atggcggcga attttcgggc gttgagcttg
     1321 cggcgcaaat attcgatgct tccggtgccg agctcgccgg cattcagcca gccgcgatag
     1381 ccgatgaaca gatagggatt gaccaggtcg ggtgtttcct gaaaaatgag actttcgttc
     1441 atgaactctc cttggaagtt attcgcggct gcggtcatca ttgcgcaccg ccggcatgat
     1501 ttttctaaaa ccaaaaccgc ttgttgatta tggttattgc gttttttgca gtcaagtatt
     1561 ttgtttcagg atgatgggtt tttgcttcaa gttgaatggt atcagccgga agaattttct
     1621 tcggttccga tatttatttt ttttgaccgg ggacagtcgt caaaggaaaa acatcaattt
     1681 tgttaaatag ttgaatcttg actggatggg agaaaaaatg cttgacagta tataaataaa
     1741 atatatatac aatctacata gtcgatgcct gaatgattga ggaaaagttg tccgtaaatg
     1801 acgccgtttg ttgaggtgaa accatgcctc gtgaagaaag gagggtgcgt accgaagaat
     1861 agaatgcgcg acggataatg gtcagcattt taaatgacaa aagcaacgat ggatgtggag
     1921 aagatgcatt tgaaatgtag tcaacgaata ctaaaaagga ggaatcatgg aatatcgatt
     1981 tgaattgcct acaaaaatga tcatcggaga aggatgttcc aagcagttgg gggaaaatgt
     2041 caagctgacc ggtgcaaaac acgtcctgtg tgtttttgat aaaggtgtgg aaggggcggg
     2101 tcttgtcgcg ccgctcatcg cggacatgga aacggcgggc ctgaaggtaa cgcctttcaa
     2161 cggtgtgctg ccggatccgc ccatggaagt gatcgatcag tgcacccaac tggcgcggga
     2221 ggccaagcct gacgtctttg tcgccgtggg cggcggcagc tccatcgaca cagccaaagc
     2281 cgtcaacgcg aatctgtcca atcccggaac gcttcgcgac catgccatca acctcaacgg
     2341 cctcaaggtg tttccttttg aaaatccgct caaacctttt tttgccctgc ccacaaccgc
     2401 cggaaccggc agtgaagtat ccccgtcggc ggtcgttacc gatcacgaag cgaagctgaa
     2461 ggtttcggtc atggcgcccg atctggtgcc caccatggcc atcatcgatc cggctctgac
     2521 cgtcggcttg ccggtgaatg tgaccgcttc caccggattg gacgcctttg ctcatgccat
     2581 ggaaggactc atgggcggtt tggcgatttt cactccgtca cccatgcggg attcttttgc
     2641 ctttaccgcc atcgaactgg tgctggagag tcttctgccg gcgatgaaga acgggaaaga
     2701 catcaaggca aggacggata tgtcctatgc cgcttttatg tccattctgg gagccggcgg
     2761 cggactgagc atgggtcatt gcctgggtca tgccatggga gaagtctgtg gcattcataa
     2821 tcatggcttt ttatgcgctt ccattcttcc ttacaacatc gagttcatgg ccgaactcat
     2881 tccccagaaa ctcaataaac tggcctccct gttcaagatc gatccgaacg gaaaatccgt
     2941 cgcccagatc gggacggcaa tcaagttggc cattcaagcc ttttacaagg attgcggcat
     3001 tccgccgctg aaagatatgg ggctgaatct ttccgacacg gaggagatca ttcaccacac
     3061 gacatcgggc acctggtatc ttctggcatc caaaaaacca tcggaagagg aactgacccg
     3121 ctggattcag gccgtttacg aaggaggaaa cgcatgaaga catacgataa aatctacatc
     3181 aacggccagt gggtttcccc ggccggcaaa gacaaagtca aagtgatcaa cccctgtacg
     3241 gaagaagcga ttgcctcggt gccgctgggc aacgaagaag acgttcagaa ggccgtcatc
     3301 gcagcgcggc aggcttttcc ttcctggtcc aaaaccagtg ttgccgatcg gatcaagctg
     3361 atttcaaaac tttccgaagc catcgccgcc cgccagaacg agatcggcga tacgatttcc
     3421 cgcgagatgg gcatgccttc cgcatggtcc cgaatgattc aggtcggcct gcccgttgcc
     3481 acgctcaaca gcttcgttcc gattctggaa aactattcct tttcgtatcc gatgggtcga
     3541 acggaaattg taaaagaacc gatcggcgtc tgcggtttca ttacgccctg gaattatccc
     3601 cttcaccagc ttgtgggcaa agtggcgccg gcgctggcgg cgggctgcac catggtggca
     3661 aaacccagcc aactggctcc cctgaatgcc tatgcattta cggagatcat gcaggaaatc
     3721 ggcgtgcctc ccggagtttt caatctggtc cccggggccg gatcgaaggt aggctacgcc
     3781 ctgtcgtctc atcccgatgt ggatatggtg tctctcacgg gttccacgaa gtccggcatt
     3841 gccgtggccc aggccgccgc cacaacgatc aagcgggtta cgctggaact gggcgggaag
     3901 tcccccaatg tcctgctcga agacgcggat attcccatgg cggtgacgaa aggggtgtac
     3961 aactgctttt tgaattccgg gcaaacctgc attgccctgt cccgcatgat cgtcccggcg
     4021 cacaaacaga aagaaatcgt cgaaatggca aaggccatcg tcgaatccat ggtgatgggg
     4081 gatgccttta cggacggcgt ctatctgggg ccgatggtgg atgacgccca gcgtcaatcc
     4141 gttcgcgaat atattcgagc cggaatccgc gaaggcgcca cgctggtcac cggcgggccg
     4201 gaaacccccg aaaaccttcc gaagggattt tttgtcaagc cgacgctctt tgccgacgtc
     4261 aaacccgata tgatcattgc gcaggaggaa atattcggtc ccgttttgtg catcattccc
     4321 taccggaccg aagaagaagc catcgccatc gcgaacaaca gcgtttacgg gctttccggc
     4381 tccgtctggt cggcggattc tgaaaaagcc agagccgcgg cgcgtcagat tcgaagcggc
     4441 caggtcttca tcaacggggc cgactttgac atcgatgctc cggccggagg gtataaacag
     4501 tccggactcg gacgcgaacg cagcaaatac ggtctggaag aatatctgga aatcaaggcc
     4561 ctgatcgcac aaaaccctta atcgtttttc aatgaaaaga tccggtccgg ccgggaaggc
     4621 atatcccggc cggacaaccc aaaaccaacg gaggaccttc atgaacaagg ttatgaccat
     4681 gcaaaaagca attgccgaac atgtccatga cggcgatttt ctatatatcg gcggctatat
     4741 ctgccggacg cctttttcgg cggttcacga actgatccgc caggggaaaa aagaactgac
     4801 tattacccgc ggcaacgcct cggatgattt cgatatgctg atcggggcgg gggcggtgaa
     4861 gcgatttatt tccacgttca tttctctcgg cgtttacggc ctggcccgct gctaccggcg
     4921 ttcgctggaa aaaggcattc cccacaagat tgaggtggag gaatatacca atttgtcgct
     4981 gcccatgatg ctgatggcgg gagcgatggg catgcccttt attcccgtca aagacatggt
     5041 ggggacggac cttatgaacg tgaagggttt tatgggagag gacaaatacc ggatgatctc
     5101 ctctcccttt gacggatccc ccgttctgct cgtgccggct ctcaagccgg atgtgggaat
     5161 cattcacgtc cagcaggcgg atgaggaagg caacgcgcag atgtggggaa tcggcggaga
     5221 ttgcaaatac ggcgccaatg cgtcgaagaa ggtcattgtg tcctgcgaac gcatcgtcag
     5281 ccgggacgtc atcgggaagg acccttcccg gacgatcgtg ccggatttca aggtggttgc
     5341 cgttgtggaa gagcctttcg cgtcgcatcc gggctatacg cccggattct atgatctgga
     5401 tttcagtttt ggttatctgt atcagcaggc ctccaatacg gaagaaggct ttcgggcgtt
     5461 tcttcaggag tggatcatgg atattcccga ccgggcttcc tatatccagc actacatcga
     5521 tcgcttcggc tatgcccagt tccggaaact ccaggcgaaa ctcgattatg gttttccggt
     5581 cagttacgct tactgaggag gtgaatacaa atgattgaac atccgaagga ctatatcaag
     5641 cccgaactga tggcctgttg cggcgccaga gaaatccatg acggtgatgt ggttgtcgtc
     5701 ggtacgggat ttcccgccat gtcggccaac atcgccaagc atacccatgc ccccggggcg
     5761 gtgatgatgc aggaatcggg ggtttatgac gccagaccgg gacgtccggc cttatccgtc
     5821 ggcgacgcgt gcctgaatcc cgggtcggcc atgatcggcg ggctgaccga tgtgatggga
     5881 atgtttctgc agggcggttg ggtggacgtg ggatttctgt ccggcgctca ggtggatcgc
     5941 tatggaaatc tgaacacgac ctgcatcggt ccctatgatt ccccgcgcag caggctgccg
     6001 ggaagcggcg gggccaatcc catcggggct ctggccaggc gaacactgat catcgtccag
     6061 catgatcccc ggcgtcttgc cgaaaaagtc gactttatta ccacccccgg ctatctcaac
     6121 ggacccggcg cccgcgaacg atggggactg cctgccgcaa ccggtccatg ggtcattatt
     6181 accaataaag gtgtcatgcg cttcgacgcg gaaaccaagg aagcttatct ggaaagctat
     6241 catccgggct gcaccctgga ggaaataagg aaaaataccg gctgggattt gaaaatctcc
     6301 gcggaggtcc acgagacgga accgcccagg gccgtggagc tgaaggcgat tcgggaagtt
     6361 ctcgatccgc accgcatgat tcgggtttat gaaggcaagg ggtatgttta aactgctctg
     6421 atgtcagagc attcatcggg ctgatggagc gatcaagata aaaggagaat ggtaaatgga
     6481 tttttcattg aatgaagaac aaagaatgtt gattcgaacg cttcagacca tgggggaacg
     6541 ggaaaaactg aaggaacgcg cggcgcagat tgaccggtcg ggggaatttc ccgttgatct
     6601 gcttgaaaaa tacggagaga tgggattgct gggaatgacc ctttcacccg actacggcgg
     6661 cggtggacag ccggcgctcc atgcccttct ggcgatcgag gaaatggcca agtacagccc
     6721 catcattgcg gggccgatct ttgagtccaa tgtagggccg gtgcgggtca tcgacctgtt
     6781 cgggaccgaa gagcaaagaa agaccattgt tccaggggtg tgcaagggag aactcagcgt
     6841 atccgtttgc atgacggagc cggaagccgg ttcggatctg acctctttga gcacaaaggc
     6901 cgttgatgac ggcgaccatt acatcctcaa cggccgaaag gtctttatca cgggcggcgg
     6961 acacgcaagc cattattttg tatatacccg tttcggagac gctcccggct acaagggaat
     7021 cggcggtctg ctggttgaaa agggaacgca gggctttacc tttggcaagc aggaagattt
     7081 tctcggcctt cgggggatgc cctcctgtga gctgatcttt gaggatgttc gcgttcccaa
     7141 gaaaaacctg gtcatcaaag ccggtgactt caataaactg atgctgacct tcgacatcga
     7201 gcggtgcggc aatgcggcca tgtgcctggg cgttgccgga gccgccctca acgagtccat
     7261 cgcctatgcc caggcccgtc aggccttcgg ccggccgatc tgcgaattcc aggcgatcca
     7321 gtttatgacc gtggacatgg ccatgaagct ggatgcggcg cgacttctcg tttaccgggc
     7381 cgccgcgggt gcggggcagg gactgccttc catttacgag gcttccatgg ccaagtgctt
     7441 tgccaacgaa atggtgattg aggtgaccaa cacggccatg caggtcttcg gaggctacgg
     7501 ctacagcaag gaattcccgg tggagcggat gctccgtgac gcccgggcgt ggggcgtggc
     7561 cggcggaacc gtgcagatgc ttcgtttgac catggccagc gttctgtatg gccgtcggtt
     7621 tgatcagcgc cgcgggaagt agagggtagg atttccggaa ccgggcaacg gtatttcacg
     7681 ttttttttcg aaccgctccg ttgtccggtt ctgaaatatt tttttacttt atcaaacgat
     7741 cgatttccat catcagcgtt tgtgtctgtt ccggcagcag gccaaagccc atgtctttcc
     7801 ggtacagaac attcaaattc tgatcgacga tcagatacac ccgtttggac aggggcaata
     7861 tccagtgatc cgcgtcgtac cttttggcga ttttgccctt ttcatcgacc agcagggtca
     7921 tgttgtgcag tccgtgtttg gcgatgaatt tccggtgtga ttcagccgaa tcccgcgaca
     7981 ggcccaatat tttcactttt ccggcttcgt attttttgat gttttgttga aactcaacaa
     8041 actggcgaat tcagaccccg gtatcgtctt tgggataaaa ggccagcacc acggcgcggt
     8101 attctttcac cgcatcgctg agcttccaca tttttccgtc ctgatccggc agctcaaagt
     8161 cggcggcttt ctgaacgggg gaagccatgt cggttttggc gtcggagccc gcgtagacgg
     8221 taaacgccag gaacacaatc acgataaaga tgaattttgt ttgtttcata atcttttcct
     8281 tttcaagttt acggttggta aaaagttaac gttcgatgtc tgccacgcca aaaaatatat
     8341 gatattcctg attcaccgtc aggtcaaacg aggcggataa acaaacgatg tctctaaaaa
     8401 taatacccgg ctcgcaattc aatgcggtag tcgttttgct tttcaccgaa ttcggtattt
     8461 tgtcctccga tcataacgcc tgcccgaaag cgaagctcaa ggttggtgac gccggtgtac
     8521 agaaactccg gcatgacgtt ggcgctagag tcgtccgtgt tgatcatgca ggtgagagcc
     8581 ggcgtgaaat acaaaatatc gaaaggctcc ttctgactca gacggatgta cagatagttg
     8641 cgcatggcgt tgggacgggc gtaaccggag tcggcaagct gagccgcgcg ttctaatccg
     8701 gtctggtctc ccgttgtttg atacgcatca aaactacggg aaaccagact gtagaaattc
     8761 tgcatgtcat cccgatcaaa cccggcgctg ttgtgataaa attcggtgat ggcggtaaga
     8821 tcaaaagagg tgagatagcg caagccgatc aggaaggtgt gcgcctgccc ttcgacagct
     8881 ttcgcagcac ccgtggcatc cagaacggtt ttctgggtat tttgtatcca cgcgtattca
     8941 ccgtgaattt caagattggt ggttatattg cgcgaaaagt cgaagccata gcggtccgga
     9001 cggcttccac cggtcataaa catcacatca atgtccgtgt catagaaaag aaaatacagg
     9061 cggccggcgg tgttgagctt gttgttttgg ccaaagtttg agttgatgtg atcataaacg
     9121 gggaaaagca ccggcgaaaa ggagactatc gtgagcggcc cgtcaaagct gcggattaaa
     9181 tcggccgttg cgacaatgta tccttccatc gcctgttcgg gatcgtccgg atctttgggc
     9241 cggtcggcaa aggcaacggg gttccaggca tagcctttgc cccatttcaa ggttttcttg
     9301 ccgacatcga tcgtaacggc tggcatcggc ttggcggacg cgtgggcttc gtaaaacgtt
     9361 gtctgctccg agccgtgcgc gtaatcgttg tactgcagat cggtattgag ccttgcgaag
     9421 acccggaaga tgctcttttc atagctgcct tccaggcgca cacggccgtt ggcttcaggc
     9481 agagcctgcc ggtttggatc atcaaaaaaa cgcagctgat aaaaagcgga attggaattc
     9541 agaccgtaaa gaacgggttt ggcttcgatg taacctgaaa aatgataggg ctttttttcg
     9601 atttcggaaa gatcaaacgt atagttctct tcggccttgg tcgcggatgg cattgccgaa
     9661 accagcaagg caagaaaaat gagtccgaat cgtttcatct agcgcaaaga ctccagattc
     9721 ggcataaaag tgagcgaaaa aacctcatcc ttgagcatcc gttttttgat ttggaccatg
     9781 atcatgacgg atttgtagcc tttgtaaagc gggctgtccg tttcgatcac cgcgggccgg
     9841 acgatgccgc cgccgaaatc cttcacattc ttgaaataaa gcgttttgat cagcatgcca
     9901 gtttcggtca gacattcgat tttcgctggc agacgtttgg ctttgtccgc cctgatttta
     9961 agctggtcat aggccacgct tttggatttg gccttgagca gcagaagata ttctccacct
    10021 tcttccttca cggccagaac atcgtattcc gcggcgtaat ccagttgcag aatgtcggcg
    10081 ttgttgaaca cgccgcccac aaccgattgc agacttgtaa tgcggatggg tttgccgaca
    10141 ttgggaatgt gaagccacat gttgtcgccc agacgaagcg tgctgcggcc tttttcgctg
    10201 gccggcgcca gaaacacgga ggccaccttg tccaaccctt ttttaactgt aaagagcgtg
    10261 aactctttct ttttcccgtc cggttcgata ttgatgagct tgcggtagga ttcgtaggat
    10321 tccggcgaaa ggttgcggtc aatctcctgt agaagctttg ttccgtccac ggcaaaggcg
    10381 gaaaacggca acatgatgat cagggcaaaa acaaggtaaa tagttttctt catttcattc
    10441 tcctttttta tacatgccgc agggcgtcga tgggctccat ccgggaggcc ttgacggccg
    10501 gctgtaggct tgccaatacg gcgactgtaa tgacaatgac tgaaaccacc agcagatccg
    10561 gtgggtcgag cgttgccttg aggagaaggc ctttttgctg gccgaagtca aagcggatgc
    10621 cgatcaggtt gagaagagcg atgagcacgg ccccgatccc gacgcccgct cccgctccga
    10681 ccacccccat gcagaatcct tccaaaatga acatggaacg aattttcccc ggcggcgttc
    10741 caatcgcggc tatcgtgccg atttcccgaa tgcgttcata aacggccatc accatgacgt
    10801 tcatgatgct gattagcaca atggcaatca gcatgatccg gatgaaaaag gtcatgatgt
    10861 caatcattcg ggcgatgttg aaaaaaggcg agaggtcctc ccacgaatgg acttcaaaca
    10921 gcggctgccc ctgcggattc acctcgccgg ccgtcgcatt ttttaaatgc tggacggcgg
    10981 cggccaggcg tccgaaatct ttgagccgga tcgccacctc gctgatttcc ggttcggtca
    11041 tgcgcagtac ctgagccgcg tcttccatac ggacataccc gtcccgtccg ccgggtcccg
    11101 tggcgctctc caggatgccg gcgacagtga actgcatgcc gttgacggac ccgtcggcgt
    11161 tggtggccac aattacgacc ggatcgccgg gtttaacctt cattcctttg gccagaagcg
    11221 cgggaatcag gatttcacct ctgccgactg ttgatttgcc ttcgagaatg cgcgagggca
    11281 aaagcggtac ggtcctgaat tccgcttcgg gatcgatgcc gttcaggcgg atattggtgg
    11341 tttcggtgaa attgctgaac atggcgccga acttgaggcg gggcgaaaag gcttccacct
    11401 gttcatccgc ggccagcgcc tgcctgattg tctgataggc gttcggtttc atgttcagat
    11461 ttaaaggcag gttgtcgatg gacgcgacat atcctttgcg gtggatctgc aaatggccca
    11521 tcatggaatc ggtgatctgg ccggtgatca tagacttgaa ggagccgctt acggagacaa
    11581 agaccagcac aaagacgacg ccgatcgtaa tgagcgaagc cgtgagcagc gtccggcgct
    11641 tgtagcggat caggttgcgg atggcgattt tgaaaagatt acgcatgcgc tgttccttcc
    11701 ttcaactctt tttcggccag gcggccgtcc acgaggcgga aaagaacttc cgcctcaccc
    11761 acgatctttg tgtcgtgcgt cgaaaaaata aaggtggttc cgagatcgtc gcgcattttt
    11821 ttcatgagcc cgatgaccgc ataggatgtc tgggaatcca gattggccgt gggttcatcg
    11881 gccagaacca gcgcgggatt cgttaccagc gcgcgggcga tggccacccg ctgcttctga
    11941 ccgccggaaa tctggtgggg gtatttgccg ccctgatcat ccattcctac cgcggcaagc
    12001 atgcgggaaa ttctttcctt gcgctcccgg aaggggatat tctggaccat gatcagggga
    12061 tattcgatgt tttcataaac cgtcagcacg gggatcatgt tgaagtcttg aaagataaag
    12121 cccagatgat ttccccgaaa ggcggcgctt tctttccggc ccagtgttcc gacatcggtt
    12181 cccgccacgg tgagcctgcc ggcagtgggt ttgtccaggc aaccgatcag gttcaaaagc
    12241 gttgtcttgc cgctgcctga cggaccgaca aaagaaacaa atgaggcggg ttcgatttca
    12301 aaattaagat tccgcaaggc cggcacggtc agttcttccg ttcggtagtc cttcgtgatg
    12361 cctgccgccg tgacgagtgc cattttcttt ctcccggctg gaatgttttt gcggcgggcg
    12421 gctcaagaac ggggccgccc gccgccggtt gtctaaaaga atcaatcgtc aaacgccatt
    12481 ttggttttcc agacttccca gaccttgccc accaggtcgg gaccgggttt gagcgttgct
    12541 ttgccgggct tccagccgga cggcgtggct tcggcgccct tggttttgcg cacgtgctgg
    12601 aaagcctgaa tctgccggat gctctccatc acgttacggc ctaccggcgg ggtaaggact
    12661 tcaaagccct gaatgatgcc gtcgggatca atgatgaacc ggccgcgggc ttcgacgcct
    12721 gcatcctgaa ggtaaacgcc aaatacatct cccactttac cgcccccgtc ggagagcatc
    12781 gggaagggga tgccgccttt gaccattttg gacagttcat gatcgttcca catcttatgg
    12841 acaaaaacgc tgtccacgct catggacaaa acttcgacgc ccagtttttg aaattcggga
    12901 tatttctctg cgaccgcaga gatttcagtg gctcaaacaa acgtaaaatc tccgggatag
    12961 aagcacagca ccacccactt ccccagatag tcggacaact tcacattgat gaatttcccc
    13021 ttctgatacg cgggcgccgt gaaatcgggc gccttctttc ccactgtgac catgcttttc
    13081 acctccttga tgacgggttc ttcggttgtt cctttttttg ccgccggttt ttctccgacc
    13141 ggtccgcccg tgggacgggc gcagccggcc ttgatttcct cagccataga ttattacctc
    13201 ctttcttgtt ttttgttatt tttctctcgg atacggccag gccatgatgc ctccttccat
    13261 gactttaacg tttttccagc cgttgccttc cagtgcgatt gccgcttcgt agccgcgcag
    13321 ggatatttta cagtagcaga tgatttcttt gttcttatcc ttcggcaatt cctgaaggcg
    13381 cttgcggatc gcgcccagcg ggatcagcgt ctcgccgatg cccagacgca tttcctcgaa
    13441 ttcctccgga cctcgtgtat cgatgataaa cggcttttca cccgctttta gcttgtcatg
    13501 aacttccttg cagctgatgc ccttgagacg tcctttcatt ttgttctgca tcaggtgagc
    13561 cgtggcgaca aaatgatcaa tcgccagcga aaaaggcggc gcgtaaggca ggtcggcatt
    13621 gacaaggtcc tcgacggtta acttgccctg aatggccatg gcccactgag caatttgttt
    13681 ggcgacattg ccgggaccga tacactgggc gcccaatatc cggccgcttt tacgctccgc
    13741 gatgagcttg gtaaggagga gcttgccttc catgaaaccc ggcttgtcgg ggctggcgtt
    13801 catcacggtt atcatgtccg aatagcccag acgtttggcg accgtttccg aaagccccgt
    13861 ggatcccgcc gcatagtcga acactttaca gatgccggtt tgaatggttc cgggaaaggt
    13921 cacacagttg gcggaagcgg cgttttctcc cgctacgcgg ccctggaggt tggccagatc
    13981 gccgtaaggc gcgtgcactt ttttgccggt aatgcggttt aacgtttcca cgcagtcgcc
    14041 cacggcgtaa atgtccggat cggaggtctg catgtactca ttgacatcaa tgccgcccag
    14101 ttcgccgatt ttcaggcccg cctctttggc cagcttgaca ttggggcgca caccgatggc
    14161 cacgaccgcc agttcgcagg gcagttccgt gccgttttgc aatttgaccg cggtgagctt
    14221 tccgtcttcg cctaagaaag cggcgatgcc gttttcggtg atgacgttgg cccctttgct
    14281 tttgacgtgg ttttcaacga ttttcgccag ctcccaatcc agaaaggtca gcagttgagg
    14341 cagaagttct atcacggtga tttcaattcc cgccagttgc agcgcttcgc aggtttcaat
    14401 gccgatcagg cccccgccga tgacgacggc tttcttgatg gtgccttcgt cgcgaatctt
    14461 acgcagaaaa tcagcgtctt tcatcgattg cagcgtcgtg atgccgtcga gctccacacc
    14521 cggcaccggc ggcatgcgcg ggacggcgcc cgtagcgatg accagtttgt cgtaggtcag
    14581 ctcgccggtt ttaccagtaa cgatattttt gaaagtgatg gaatgatttt tagtgtcgat
    14641 gcctgtgact tccgtgttta cttccgcgtc aatgtctttg gcgttgaggt aaaagatcgg
    14701 atttcgcaca acgccggtgg gggtgcacaa aagttgattg cggtcattga aaaagccgcc
    14761 cacgtaatac ggatagccgc aggacgccat cgaaagatcc gcgtcttttt gcagaatgat
    14821 gacttccgcg tcattgtcga tccggcgggc tttggccgcc gccttggggc cggcggccga
    14881 tccgccgatc acgatgattc tttttcggtt tgccatgaat tgctcctgtt gttgaaaatg
    14941 agtaaatttt ttcaaatggt tatagtgaat ggttcaattt tcttgaaaaa tacttaacag
    15001 atggcggaag ccattgcatt gatctatgtc aacgagccgg acgtttataa aaaccattcc
    15061 ggttcccggc gaggggaagt aaaggcaacc tctaattcct gtttgtgcca caggtaattt
    15121 tcgtcgtcaa aatactttgc ctccaccggg cagtttttga tgcaggcgca gcatttgatg
    15181 caaatccccg tgatgatgga ttcatcatcc gcatcaatgg accccacggg acaaagactg
    15241 acgcagaggc
//
LOCUS       DAOSIM010000022        15223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_4028_length_15223_cov_7.668117, whole genome shotgun sequence.
ACCESSION   DAOSIM010000022 DAOSIM010000000
VERSION     DAOSIM010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 15223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15223
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4028_length_15223_cov_7.668117"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLR47_02045"
     CDS             complement(<1..235)
                     /locus_tag="PLR47_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG11746.1"
                     /translation="MSLTISVETKRPGFWFVSLIGRLDQTTAGECDEKITSILLPETR
                     AIVFDMTGLNYISSMGLRIILKTRRFMSGQGGAV"
     gene            complement(279..1352)
                     /gene="hisC"
                     /locus_tag="PLR47_02050"
     CDS             complement(279..1352)
                     /gene="hisC"
                     /locus_tag="PLR47_02050"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOG11747.1"
                     /translation="MSRFWSQTAKSIKPYVPGEQPKDRKYIKLNTNENPYPPSPKVLD
                     AIRRAADETLRLYPDPSADALRDAVASSFGIERENVFIGNGSDELLAFCFPAFFEPAG
                     LPILFADVTYSFYPVYAQLFHIPCRLIPLDEEFRLPVEGYLQENGGILIANPNAPTGM
                     GVPLETIERILRHNENSVVVLDEAYVDFGGESSVGLISRFPNLLVIRTLSKSCSLAGL
                     RVGFALGGKELIEGIIRIKDSINSYTIDRLAQAGAREAIGDAAYFEETKSKIVRTRGR
                     VAQALVNFGFRVLPSQANFLFIGHPQQPARVLFQKLREQGILVRFFDKPRIDRFLRVS
                     IGTDGEMDLFLEVVARICEKPAE"
     gene            complement(1423..2334)
                     /locus_tag="PLR47_02055"
     CDS             complement(1423..2334)
                     /locus_tag="PLR47_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOG11748.1"
                     /translation="MTFKGIITVIRIPFLILALTLGVLGAAVAWYESRLFGSPFYFGY
                     ALLATFGLLVAHAGVNIFNDYFDARSGLDYKTQRTPFSGGSGAAPTGQLTVSRILWLG
                     IACFAILIPIAWFFVVKSGWMLLPLLAAAAALIVFYTPFILKMGYPEWSPGLGLGILP
                     VMGAYFVHTGAYSMSAFIASMPSFFLVHNLLLINEFPDVEADITVGRRTLPIVAGKKN
                     AAYFFSFFTVLVYCWVIGAVLFGSMPAFTLLSVLTFPIAVRVIKRALRYDDRETLLKA
                     MSANVLLVLLTQALIAAGYILSGIFKI"
     gene            complement(2427..3044)
                     /locus_tag="PLR47_02060"
     CDS             complement(2427..3044)
                     /locus_tag="PLR47_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOG11749.1"
                     /translation="MCGRFLLITDLSVVAQSFGAADASLPFLPERNIHPGRHIPAVIR
                     TAGGNRLASFLWGLVPFWAKDPSIGARLINARAETIAEKPSFQNAFRKRRCLIPADGF
                     YEWKKDGTPKKLFQFGLKSKEPFVFAGLYERWAPAGQKPLDTCTIITTRANEVVAPVH
                     DRMPVIVPKAGQDLWLNPEIQNPALLAEVLKPYPAREMTGEPATF"
     gene            complement(3063..>3626)
                     /locus_tag="PLR47_02065"
     CDS             complement(3063..>3626)
                     /locus_tag="PLR47_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOG11750.1"
                     /translation="INEPTLVLLSKILEAGHPTPEEFDAAFKPLMAMAPFELVDYVGI
                     DVAAHGLEYFAQVLSPDYKPSEAMMSYLKINHLGKKTGRGFYDWSAGRPAIDLAKATK
                     EYDINQLIALQVNEATKLLEEGVADDPKEIDLAMANGGGSPFGPFALAQGIGYPELVF
                     KLGEIYKKFPLDIFKPTNTLKEGKIKL"
     assembly_gap    3627..3729
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3730..4194)
                     /locus_tag="PLR47_02070"
     CDS             complement(<3730..4194)
                     /locus_tag="PLR47_02070"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOG11751.1"
                     /translation="MKAEDVKKIAMIGAGDMGHGIAASCLLGGYTVVLRDIDQKFVDK
                     GVAGIIKSLEKFKEKGKITPDAFAHALVRLIPMVDLKEAVKDADFIIEAVPEKLEIKK
                     SVFAEIDQYAPKHAILASNTSNISITEIASATQRPDKVIGYHFFNPAILMKLV"
     gene            complement(4260..5930)
                     /locus_tag="PLR47_02075"
     CDS             complement(4260..5930)
                     /locus_tag="PLR47_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOG11752.1"
                     /translation="MAERPWQKFYDYYVPQTIRYPQFPAQRIFQLTAGVQPNKVCTSF
                     YGTELTFWQVREQVLRLANALAAQGVKKGDRVGIHLPNCPQFIVAYLAVMHLGAIVVN
                     MNPLYTVNELQFIVENTSMETMITFDMFLDNVRKTAAATGLRRVIVTKITDYINGLGV
                     STAKSLDLEEGWLHFSELIENCKDTRPPRVPIVPSDPAMIQFTGGTTGFPKGALLTHG
                     NLVAATFQAGLWASPGSYSPHSMPHQERSALCVLPFFHVYGNVVAMNWSFFGCATQVI
                     VPRFDIDEILDTIVKAGHITYFPAVPTMITAIVNHPKAANLDLGRYIGLLNSGGAPMP
                     VELIERVIDLGITFNEGYGLSETSSITIANPLAYNKAGSIGIPMADNDFRLVDVENGV
                     EDVKPGEPGELLVKGPTVMKEYWNNPEETRNQLRDGWLHTGDIAQVDEDGFIFIVDRK
                     KDMIIAGGFNIYPREIEEVLYEHPKVAEAVAVGIPHDYRGETVKIFVVLKSGEKSSEE
                     EIIDYCKQKMTAYKVPKIVEFREAVPKSAVGKILRKVLRDEELAKLKK"
     gene            complement(6040..7914)
                     /locus_tag="PLR47_02080"
     CDS             complement(6040..7914)
                     /locus_tag="PLR47_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOG11753.1"
                     /translation="MSLNQLVDSRDVRFTLFEMLELEKLNRFDRLKEFDRPVYEDTLE
                     LAEKIALQQFYTSNRDGDTVGLKFDPKTNSVKVPESFHKGFNAYIEAGFHSLAFPQEI
                     GGMGMPSSISMAAAEYFNAGNTALTMYCGAITGAIHIIQSFGSEEVKNTFLPKMFEGK
                     WGGTMCLTEPGAGSDLGALKTKAIRQPDGTFLISGQKIFISNGEHDMTENIIHPVLAR
                     IEGDPEGTKGISIFVVPKFLVNPDLTLGERNDVYCTGIEHKMGLKGNATSTLNFGDNG
                     KCIGYLLGKECEGMKVMFMLMNAARIHTGVQAEAVSSAAYMHAITYARNRVQGVHITQ
                     SMNPKAPRVAIIEHPDVKRMLLYMKSTIEGMRMLCLFLAYHEDIMHYSQSPEEVKTAT
                     GIVEILTPIVKAGISDAAWLVTAEAMQVYGGYGYCQEYPVEQYARDVKVFSIYEGTNA
                     IQSLDLQMRKILMNKDMYNYQMLKKYFADTVARAKGIVDERYVAPVERGIAKFDEIIS
                     MMVKQLQEGKFLALVANATPMQQAMFPLIVAWLHLWSLTITLPKSRELLGDAKGADRK
                     KILETNPEAAYYNGRVLSAQFFIGAEFPKFFGRVECVMNNEGAAIKVESENFTGALKE
                     "
     gene            8029..9375
                     /gene="rsmB"
                     /locus_tag="PLR47_02085"
     CDS             8029..9375
                     /gene="rsmB"
                     /locus_tag="PLR47_02085"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOG11754.1"
                     /translation="MKKTIRHQAVEILTRVSKSGAFAADLLDSRLEKQSLSQTADGRL
                     LTHLVYGVLRMRGRLDWILTRLYHGNYSKMEENIKNILRTGLYQLQFSDRLPAFAVVD
                     EAVKIARLFHPQAGGLVNAVLRSYLRSPDSWSFPDRDRNPAQFIAAFHSHPLWLVEEW
                     LNLFGREETQALCAADNEMPPLTVRVNLLKSGRRELMEKLIQAGFQVEETPISPDGIR
                     VSFSPSPIQKTAFFAEGLLRLQDEASQLIAYLVNPQKGQKILDVCAGSGGKATHLAAL
                     MKNEGRIVALDRNPEKIEQLRNNASKMGIGIVEAARSDGEIPLSSEYHNQFDAVFVDA
                     PCSGTGTLRRNPEIKWRLHAGDIERYAGAQKSILRNAAAAVSRNGSLVYVTCSLLSRE
                     NDEVVRQFLTDCPHYCLCPPNSHVVQNLMDPHGILRTYPHRTSMDGFFGAVFRRRI"
     gene            9437..10066
                     /locus_tag="PLR47_02090"
     CDS             9437..10066
                     /locus_tag="PLR47_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG11755.1"
                     /translation="MFIQQMQVGHMAVFAYLVGDPVTGDALVIDPADNAHEILAEASK
                     NKLRINYIVNTHGHVDHIGGNAQMKKLTGAKIIIHEDDAFMLTSTPASMLRMFGAAQS
                     PPADLLVSDGQIISTGNIELKVLHTPGHSPGGMCLYAPGCVFTGDTLFVEAVGRTDLP
                     GGSWQTMYQAIKTKLFTLPDDTKVLPGHNYGRTPTSTIGHEKLHNPSVR"
     gene            10083..10964
                     /locus_tag="PLR47_02095"
     CDS             10083..10964
                     /locus_tag="PLR47_02095"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOG11756.1"
                     /translation="MHKTIAQTHFPKLKFVKRGKVRDIYAVKDYLLLVATDRISAFDV
                     IMPDPIPGKGELLNKMSLFWFKKMEDIIGNHVVSADAADFPEECAPYAEILQGRSMLV
                     EKATPLPVECIVRGYLSGSGWQEYRRDRSVSGIQLPAGLKESCKLPQPIFTPTTKAPQ
                     GEHDSPITRSEMEDIISAEITERIIQKALAIYERAAAIALAAGIIIADTKMEFGLIGK
                     KLILIDELLTPDSSRFWPADDYEEGRPQKSFDKQFLRDYLLSIVWNQKPPAPELPPDI
                     IGKTRAKYEEAYSRLVK"
     gene            11052..11450
                     /locus_tag="PLR47_02100"
     CDS             11052..11450
                     /locus_tag="PLR47_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11757.1"
                     /translation="MAGKKLPDYRLKQKILYIDKTSPESLISYGDLYWEAGAPSDALD
                     FYARASHSAGLQKIRDAALQNGDVFLFHGAARALGLELRDEDWENIARTAAALQKFSF
                     SVQASKKISNAERTDALAAWIKAEEAKQSA"
     gene            11447..12511
                     /gene="dnaJ"
                     /locus_tag="PLR47_02105"
     CDS             11447..12511
                     /gene="dnaJ"
                     /locus_tag="PLR47_02105"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOG11758.1"
                     /translation="MTKRDYYEILGVGKNATDEELKKCYRKIAMQCHPDRNPGDKQAE
                     ERFKEAAEAYEVLSDRQKRETYDQFGHAGLSNSGFQGFSGFDDVFSGFGDIFEDVFGY
                     GRGRGGRRTRPAARAGADLRYDLKISFLDAAFGITTAIELEKLHTCHECEGTGAAAGT
                     TPSTCPTCHGRGQVVQSSGFFTISSTCPHCNGHGRIIAKPCHACRGTGQEKRRKTVEL
                     KIPAGVETGSRLRLRGEGEAGELGGPSGDLYVFLHVEDHEFFLRSEDDISCRIPISIV
                     QAALGATIEVPTLKDTEKIKIPRGTQTGQTFRLKGKGIPHLQGYGRGDQIIEVFVRIP
                     TELTRKQEDLLREFEKSSRQ"
     gene            12576..12977
                     /locus_tag="PLR47_02110"
     CDS             12576..12977
                     /locus_tag="PLR47_02110"
                     /inference="COORDINATES: protein motif:HMM:NF023514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HOG11759.1"
                     /translation="MKSKIGRLVVCMFALMSLAGCGALVAGGAVVGAGAGTYLYANGE
                     LKTDYAASFDTVWSACEKTVAEMRGIQVQPTREIAKGTISAVINDENVKIDIAYRAKE
                     QTTVGVRVGLIGNKLSSQVIHDKIADQLAKR"
     gene            13018..14004
                     /locus_tag="PLR47_02115"
     CDS             13018..14004
                     /locus_tag="PLR47_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOG11760.1"
                     /translation="MNKKFLFGILLGVVLIYFSIRGIHFNDTLASLKKIHLGWAALSL
                     FFIVFMQALRSYRWGVILEPMEKISQGTLFAVTSVGFLAIAAIPARIGELARPYLISK
                     KSGVKMSSALGTIVVERVLDSLAVLTITIAVLIAQHDLPRWMFTSGILFFLLTLMMIV
                     FIVGLVWRRETAVSVIDWILRRLPGALAQKVNPLIHHFIDGFQVITDVKRLLSLLALS
                     AVVWLVDVAAIYTLLMAFGLNLPVLAAFVVMVILIAGIAIPTAPGFIGNWHYACILGL
                     SLFGIAKPQGLSFALVYHFLSMMVVVVLGAAFLPFNKFSLSEMTKQMKKTED"
     gene            complement(14153..>15223)
                     /locus_tag="PLR47_02120"
     CDS             complement(14153..>15223)
                     /locus_tag="PLR47_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG11761.1"
                     /translation="FIEKPLSLDKVMILVNNALNLVRLEEENILLKQKVSHQYELTGT
                     SPVIVELKEMIGIVAPTNAWILIMGENGTGKELVARSIHHLSRRSHKPIVEVNCAAIP
                     EELIESELFGHEKGAFTGATEKKRGKFDLAHEGTIFLDEVADMSLKAQAKILRILQEK
                     KFERVGGNKLIDMDVRVLAATNKDLEKEMEAGRFRQDLYYRLHVIPLRVPPLRDRKED
                     IIHLTERFLLDFAAKEGQPLKTLTAAALEKLMVHDWPGNVRELKNIIERLIILTPSNE
                     IRAEDIPELSVKADMDAPSFEETAAAGDSLRDARLDFERQFILKKLEEYDGNISKTAE
                     AIGLERSNLHKKLKALKVPVKE"
BASE COUNT         3268 a         4297 c         4211 g         3344 t
ORIGIN      
        1 aaacggcgcc gccctggccg gacatgaatc gcctcgtttt caaaatgatg cgcaggccca
       61 tgctggaaat ataattcagg ccggtcatgt cgaagacgat ggccctggtt tcgggcagca
      121 ggatcgacgt aatcttttcg tcgcattctc cggcggttgt ctggtcaagc cgtccgatga
      181 gcgaaacgaa ccagaacccg ggccttttgg tttccacgct gatcgtgaga gacattgcct
      241 ttctccttat cgtgaaatcg aaacgccgcc cgtgaaaatc attccgcggg cttttcgcaa
      301 attcgtgcga cgacttccag aaagaggtcc atttccccgt cggttccgat gctcacccga
      361 aggaaacgat cgattctggg cttgtcgaaa aagcgcacaa gaatcccctg ctccctcagc
      421 ttttggaaaa gaacgcgggc gggttgctgc gggtggccga taaatagaaa gttggcctgc
      481 gacggaagaa cccggaatcc gaaatttacc agcgcctgcg ccaccctgcc gcgggtccgg
      541 acaatcttcg attttgtttc ctcaaaatac gcggcgtccc cgatggcttc ccttgcgccc
      601 gcctgggcca gacggtcaat cgtgtaggag ttgatggagt ctttaatccg gatgattccc
      661 tcgatcagct cttttccgcc cagggcaaat cccacccgca gtccggccag cgaacaggat
      721 ttggacagcg ttcggatcac cagcagattc ggaaagcggc tgatcaggcc gacggacgat
      781 tcgcctccga aatcgacata ggcttcgtcc agcaccacga cgctgttttc attgtgccgc
      841 aaaatccttt cgatggtttc aagcggcaca cccatgccgg tgggcgcgtt gggattggca
      901 atgaggattc ccccgttttc ctgaagatag ccttcaaccg gcaaacggaa ttcttcatcg
      961 agcggaatca aacgacaggg aatatgaaaa agctgcgcgt acacgggata gaagctgtag
     1021 gtgacgtcgg caaaaagtat gggaaggccc gccggctcga aaaaagccgg aaagcagaag
     1081 gccaggagtt catccgagcc gttgccgata aacacgttct ccctctcgat gccgaaggac
     1141 gaggcgacgg cgtctctcag cgcatcggcc gacggatccg gataaagccg cagcgtctcg
     1201 tcggccgcgc gccggatggc gtcgagaacc ttcggtgaag gcgggtacgg gttttcgttg
     1261 gtgttgagct tgatgtattt ccggtctttt ggctgttctc ccggaacata aggcttgatg
     1321 cttttggctg tctggctcca gaaccggctc atatccgctc atccgttggt tgtcttttta
     1381 cactttgcag atttcatata ggaaacgcgc caaaatttcc attcatatct taaagatgcc
     1441 gctcaaaatg tatcccgccg cgatcagcgc ctgggtaagc aggaccagca gaacattggc
     1501 ggacatggct ttaagcagcg tttccctgtc gtcataccgc agggctcgtt tgatgacacg
     1561 aacggcaatg ggaaaggtca gcacggacaa aagcgtgaag gcgggcatgg aaccgaagag
     1621 gaccgcgcca atcacccagc aataaaccag cacggtgaag aaagagaaaa aataagccgc
     1681 atttttcttt ccggcgacga tcggcagcgt ccgcctgccc acggtgatat ccgcttccac
     1741 gtccggaaac tcattgatca aaagcaggtt atgcaccagg aaaaatgaag gcatcgaggc
     1801 gatgaaggcg ctcatcgaat acgcgccggt gtgaacgaaa taggcgccca tgaccggcag
     1861 gatgcccaat cccaggccgg gcgaccactc ggggtatccc atcttgagga tgaacggggt
     1921 gtaaaaaaca atcagagcgg cggccgccgc aagcagcggt agcagcatcc agcccgattt
     1981 cacaacaaag aaccaggcga tcggaatcag gatggcaaag caggcaatcc ccagccacag
     2041 gatccgggaa accgtcagtt ggcccgtcgg cgccgcgccg cttccgccgc tgaaaggggt
     2101 gcgctgcgtt ttataatcca ggccgcttcg ggcatcgaaa taatcgttga aaatattcac
     2161 gcccgcgtgg gccaccagaa ggccgaacgt ggccagaagc gcgtagccga aataaaacgg
     2221 cgagccgaag agccgggatt cataccaggc gacagcggct cccagaacgc ccagcgtgag
     2281 cgccaggatc agaaaaggga tgcgaataac ggtgatgatg cctttaaatg tcatgagttt
     2341 gtccgttgac aatcctttct gtgaaatcgg actgctcttt agcagaggat gcctcttctt
     2401 ggcaaaccaa ttcttcagga catccgctaa aaagttgccg gctcaccggt catttcccgg
     2461 gccggatacg gtttcaatac ttcggcaaga agggcgggat tttgaatttc cggattcagc
     2521 cacaggtcct gcccggcctt gggcacgatc accggcatgc ggtcgtgaac cggcgctaca
     2581 acctcgttgg ctctcgtcgt gatgatcgtg caggtgtcga gcggtttttg accagcgggc
     2641 gcccaccgct catagaggcc ggcaaaaaca aacggttctt tcgatttcag tccgaattgg
     2701 aaaagttttt tcggcgtccc gtcttttttc cattcgtaaa atccgtcggc gggaatcaga
     2761 catcgtcgct tccggaaggc gttttggaag gagggttttt ccgcgatcgt ctccgcgcgg
     2821 gcattgatga gcctcgcgcc gatggacggg tctttcgccc agaagggaac cagcccccac
     2881 aaaaaagaag ccagcctatt cccgccggcg gtacggatca ccgcgggaat atgccggccc
     2941 ggatggatgt tgcgctcagg caaaaacgga agggaagcat cggccgcgcc aaagctttgc
     3001 gcgacaaccg ataaatccgt gatcaacaaa aaccttccgc acatgggctt tttagatttg
     3061 cctcacagtt tgatcttgcc ctctttcagg gtattcgtgg gtttgaagat gtccaggggg
     3121 aattttttgt aaatttcacc cagcttgaac accagctccg ggtacccgat gccctgggcc
     3181 agcgcaaagg ggccaaaggg cgagccgccg ccgttggcca tcgccagatc gatctccttg
     3241 gggtcgtccg caacgccctc ttccagaagc ttcgtggctt cgttgacctg cagcgcgatg
     3301 agctggttga tgtcgtattc cttggtcgcc ttcgccagat cgatggcggg cctgccggcc
     3361 gaccagtcat aaaatcccct gccggttttc ttgcccagat ggttgatttt caggtaggac
     3421 atcatcgcct cggagggctt gtagtccggg gacagaacct gcgcgaaata ctccagtccg
     3481 tgcgcggcca cgtcgatgcc gacatagtcc accagttcaa acggcgccat cgccatcagg
     3541 ggcttgaacg cggcgtcgaa ctcttccggc gtcggatggc ccgcttccag gattttggac
     3601 agcagaacca gcgtcggttc gttgatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnc accagcttca tcaaaatggc cggattgaaa aaatgatagc cgatgacctt
     3781 gtcgggccgc tgcgtggcgg aggcaatctc ggtgatgctg atgttggaag tattggaggc
     3841 cagaatggcg tgcttcggcg cgtactggtc gatttcggca aaaacgctct ttttgatttc
     3901 cagcttttcg ggcacggcct caataatgaa atccgcgtcc tttacggctt ccttcaggtc
     3961 caccatcggg atcagccgca caagcgcgtg ggcaaaggca tccggtgtga tcttgccctt
     4021 ctctttgaat ttttccaggc tcttgatgat cccggcgacg cctttatcga cgaacttctg
     4081 atcgatgtcg cgaagaacga ccgtatatcc gcccagcagg caggacgccg cgattccgtg
     4141 ccccatatcc cccgcgccga tcatggcaat cttttttaca tcttcagctt tcatgttgca
     4201 tctccttttc tgaaatgaat aatcaggcgg gaaacggttt cgtttcccgc gattgtgcct
     4261 cattttttca acttggccag ctcttcatcc cgcaggacct tgcgcagaat cttgccgacg
     4321 gccgacttgg gaacggcctc acggaattcc acgatcttgg gcaccttgta ggccgtcatc
     4381 ttctgtttgc agtaatcgat gatctcctcc tcgctggatt tttcaccgga cttcaggacg
     4441 acaaatatct tcaccgtctc gccgcggtaa tcatgcggaa tgccgacggc caccgcttcg
     4501 gccaccttgg gatgctcgta gaggacctcc tcgatctccc gcggataaat attgaagccg
     4561 ccggcgatga tcatgtcttt cttgcggtcc acgataaaga tgaagccgtc ttcatccacc
     4621 tgcgcgatgt cgccggtgtg cagccagccg tcgcgaagct ggtttctggt ttcctccgga
     4681 ttgttccagt attccttcat gacggtcggc cccttgacga gcagttcgcc aggctcgccc
     4741 ggtttgacgt cttccacgcc gttttccacg tccaccaggc ggaaatcatt gtccgccatg
     4801 ggaatgccga tggagccggc cttgttgtag gcaagcggat tggcaatcgt aatggagctg
     4861 gtttcgctca ggccgtatcc ctcgttgaag gtgatgccca ggtcgattac gcgttcgatc
     4921 agctccacgg gcatcggcgc gccgccggaa ttcagcaggc cgatgtaccg gcccagatca
     4981 agattggccg ccttgggatg gttgacgatc gcggtgatca tggtgggaac cgccgggaaa
     5041 taggtgatat gcccggcctt gacaatcgtg tccagaattt catcgatgtc aaagcgcgga
     5101 acgatcacct gtgtggcgca cccgaaaaac gaccagttca tggccacgac attgccgtaa
     5161 acatggaaaa agggaagaac gcacagagcg ctgcgttcct gatgcggcat cgagtggggc
     5221 gagtagctcc cgggcgacgc ccacagaccg gcctgaaagg tggcggccac caggttgccg
     5281 tgcgtcaaaa gcgcgccctt gggaaagccc gtcgtgccgc cggtaaactg aatcatcgcc
     5341 ggatccgaag gcacaatcgg cacgcggggc ggacgcgtgt ccttgcagtt ttcgatcagt
     5401 tcggaaaaat gcagccagcc ttcctccaga tcgaggcttt tggcggtgct cacgcccagc
     5461 ccgttgatgt agtccgtgat cttcgtcacg atgacgcgcc tgaggcccgt tgccgcagcc
     5521 gtcttgcgga cgttgtccag aaacatatca aacgtgatca tggtttccat cgaggtgttc
     5581 tccacgatga actggagttc gttcaccgtg taaagcggat tcatgttcac aacgattgcg
     5641 cccagatgca tgacggccag gtaggcaacg ataaactgcg ggcagttggg cagatgaatg
     5701 cccacgcggt cgcctttttt cacgccctgc gcggccagcg cgttggccag ccggagcacc
     5761 tgctcgcgga cctgccagaa ggtcagctcg gttccgtaga aactggtgca gaccttgttc
     5821 ggctgaacgc cggcggtcag ctgaaagatc ctctgcgccg gaaactgggg atagcggatt
     5881 gtctgaggga catagtagtc ataaaatttc tgccagggtc tctctgccat cttgtaaacc
     5941 tcctttgcca gtcgtgatca gacgcgcgga gcggtcccgc cggtcccctc cccgcgacgg
     6001 ttgttgtcag cctctttatt cccggtgttt caggcgtcgc tactccttca gcgcgccggt
     6061 gaagttttcg gattcgacct tgatcgccgc gccctcgttg ttcatcacgc actccacccg
     6121 gccgaagaat tttggaaatt ccgcgccgat aaaaaactgc gccgagagca cccggccgtt
     6181 gtagtaggcg gcttcgggat tggtttccag aatctttttg cggtccgcgc ctttggcgtc
     6241 gcccagaagc tccctggact tcggaagggt aatcgtcagg ctccacaggt gcagccaagc
     6301 cacgatcagc gggaacatcg cctgctgcat cggcgtggcg ttggccacca gcgccaggaa
     6361 cttgccttcc tgaagctgct tcaccatcat gctgatgatt tcatcgaatt tcgcgatgcc
     6421 gcgctccacc ggggcgacgt accgctcgtc cacgatgccc ttggccctgg caacggtgtc
     6481 cgcgaaatat ttcttcagca tctgataatt gtacatatcc ttgttcatca ggatcttgcg
     6541 catctgcaga tccagcgact gaatcgcgtt ggtgccttcg tagatcgaga aaaccttgac
     6601 gtcgcgggca tactgctcga ccgggtattc ctggcagtag ccgtagccgc cgtaaacctg
     6661 catggcttcc gccgtcacca gccaggcggc gtccgaaatg cctgctttca cgatgggggt
     6721 caaaatttca acgatgcccg tcgccgtctt cacttcttcg ggcgactggg agtaatgcat
     6781 gatgtcttca tgataggcaa ggaagaggca gagcatgcgc atgccctcga tcgtgctctt
     6841 catatacagc agcattcttt tcacgtccgg gtgctcgatg atcgcaaccc ggggcgcctt
     6901 gggattcatg gactgcgtga tatggacgcc ctggacgcgg ttgcgggcgt atgtaatggc
     6961 gtgcatgtaa gcggctgagg acacggcctc ggcctgaacg ccggtgtgga tgcgggccgc
     7021 gttcatgagc atgaacatga ccttcatccc ttcgcattcc ttgcccagaa ggtagccgat
     7081 gcacttgccg ttgtcgccga agttgagcgt cgaggtggcg ttgcccttga gccccatctt
     7141 gtgttcgatg ccggtgcagt aaacatcgtt tcgctcgcca agcgtcaggt cggggttgac
     7201 caggaacttg ggcacgacga agatggaaat gccctttgtc ccttccgggt cgccttcgat
     7261 gcgcgccagc acgggatgga tgatgttttc cgtcatgtca tgctcgccgt tggagatgaa
     7321 gattttctgg ccggaaatga gaaacgtgcc gtcgggctgc cgaatggcct tggtcttgag
     7381 cgctcccagg tcggagcccg cgccgggttc ggtcaggcac atcgtgccgc cccatttgcc
     7441 ttcgaacatt ttgggaagaa aagtgttttt cacttcttcg gaaccgaacg actgaataat
     7501 gtggatggcg cccgtaatgg cgccgcagta catggtcagc gccgtgttgc ccgcgttgaa
     7561 atactcggcc gccgccatcg aaatcgacga gggcattccc attccgccga tttcctgcgg
     7621 aaacgccagc gagtgaaaac ccgcctcgat ataggcgttg aatcccttgt ggaaggattc
     7681 cggaaccttg acggaattgg tcttgggatc aaacttcaat ccgactgtgt cgccgtcgcg
     7741 gtttgacgta taaaactgct gcagggcgat tttttcggcc agttccagcg tatcctcgta
     7801 caccgggcga tcgaattctt tcagacgatc aaaccggttg agtttttcca gctccagcat
     7861 ttcaaacagg gtaaaacgga catcacggga atcgactaac tgatttaacg acatacctgc
     7921 ctcctttgat aattttacaa cgagtgtttg ttgtttttct taacagaaca attgcgaaaa
     7981 gcaagaaata tattgtgaat caaaccgggt tttactatac gagacaaaat gaagaaaacc
     8041 attcgacatc aggccgtgga aatcctgacc cgcgtttcaa aaagcggcgc tttcgcagcc
     8101 gatctgctgg attcccgcct tgaaaaacaa agcctctccc aaacggcgga tggccgtctg
     8161 ctcactcatc ttgtctatgg cgttttgaga atgcgggggc gcctggactg gattctgacc
     8221 cgtctgtatc acggcaacta ctcgaaaatg gaagagaata ttaaaaacat cctgcgcacg
     8281 gggctgtatc agcttcaatt cagcgaccgg ctgccggcct ttgcggtcgt ggatgaagcg
     8341 gtaaaaattg cccggctctt tcatcctcag gcgggcggac tggtcaacgc cgtcctgcga
     8401 agctatctgc gcagcccgga ctcctggtcc ttccccgaca gagaccggaa cccggcccaa
     8461 tttatcgcgg cgtttcattc ccatccgctg tggctggtgg aagagtggct gaatcttttc
     8521 ggccgggagg aaacacaggc actgtgcgca gccgacaatg aaatgccgcc tttgacggtt
     8581 cgcgtcaatt tgctgaaatc cggccgccgg gaactgatgg aaaaactgat ccaagccggt
     8641 tttcaggtcg aggaaacccc aatctcaccg gacggcatcc gtgtttcttt ttctccttcg
     8701 cctattcaaa aaaccgcttt tttcgccgag gggcttctgc gccttcagga cgaggcttcc
     8761 caactgattg cctatctggt caatcctcaa aaaggtcaaa agattctaga cgtttgcgcg
     8821 ggaagcggcg gcaaggccac gcatctggcc gccctcatga aaaacgaggg ccggatcgtt
     8881 gccctggacc ggaatccgga aaaaatcgag cagttgcgaa acaatgcctc gaaaatgggg
     8941 atcggcatcg tggaggcggc ccggtcggac ggagagattc ctctatcctc agaatatcat
     9001 aatcaatttg acgctgtttt tgtggatgct ccctgctccg gaaccggcac gctgcggcga
     9061 aatccggaaa tcaaatggcg attgcacgca ggcgacattg agcggtatgc aggcgcgcaa
     9121 aaatccattc tgcgaaacgc ggcggcggcg gtttcccgaa acggctctct ggtgtatgtc
     9181 acctgctccc ttttatcccg ggaaaacgac gaagtggtcc gccaatttct aacggattgt
     9241 ccgcattatt gtctttgtcc gccaaactcc catgtggtgc aaaacctgat ggatcctcac
     9301 ggaatattaa ggacttaccc ccatcgcact tcgatggacg gttttttcgg cgctgttttc
     9361 cggcgccgga tttaatttga cggattccac gcgatcatgc tagcttacca acccgttaca
     9421 gtgaggagag gaaaccatgt ttattcagca aatgcaggtg ggccatatgg cggttttcgc
     9481 ctatctggtg ggggaccccg tcaccgggga tgcgctggtc atcgatccgg ccgacaacgc
     9541 tcatgaaatt ctcgccgaag cgtcaaaaaa caagctgcgc atcaattaca tcgtcaacac
     9601 gcacggccac gtggaccaca tcggcggcaa tgcccagatg aaaaaactca cgggcgccaa
     9661 aatcatcatc catgaagacg acgccttcat gctcacctcg acgccggcct ccatgcttcg
     9721 aatgttcggg gcggcgcaat ctccgccggc ggaccttctc gtttccgacg gtcagatcat
     9781 ttccacgggc aacatcgagc tcaaagttct ccacacgccg ggccattccc ccggcggaat
     9841 gtgcctttat gcgccgggct gcgtttttac gggcgacacg ctgtttgtgg aggccgtcgg
     9901 ccggaccgat ctgcccggcg gctcctggca aaccatgtat caggccatta aaacgaaact
     9961 ctttacgctg ccggacgata ccaaagtgct gcccggccac aactacggcc gcaccccgac
    10021 gtccaccatc ggccatgaaa aattacacaa tccatccgtt cgataaagga aaaaagcgac
    10081 ccatgcacaa aacgattgct cagactcatt ttccaaaact gaaattcgtc aaacgcggca
    10141 aggtgcgcga catctatgcc gtgaaagatt acctgctttt ggtggccacg gaccgaatct
    10201 ccgcttttga cgtgatcatg cccgacccga ttccgggcaa aggcgagctc ctgaacaaaa
    10261 tgtccctgtt ctggtttaag aagatggaag acattatcgg caatcatgtc gtgtccgcgg
    10321 atgccgccga tttccccgag gagtgcgctc cctacgccga aatcctgcag ggacggagca
    10381 tgctggttga aaaagcgacg cccctgccgg tggaatgcat tgtgcggggc tacctgtccg
    10441 gctcgggctg gcaggaatac cgccgcgacc ggtccgtttc cggcatccaa ctgcccgcgg
    10501 gcctgaagga atcctgcaaa ctgccgcagc cgattttcac gccgacgacc aaagcgcccc
    10561 agggcgagca cgactccccg atcacgcggt cggaaatgga ggacatcatc agcgccgaaa
    10621 tcaccgaacg gatcattcaa aaagccctgg ccatatacga acgggcggcg gcgatcgccc
    10681 tggcggccgg gatcatcatt gcggacacca aaatggaatt cggactcatc ggcaaaaaat
    10741 tgattttgat tgatgaattg ctgacgcccg actcgtcgcg tttctggccg gccgacgatt
    10801 acgaagaggg gcgcccgcag aaaagcttcg acaagcagtt tctgcgggat tatctgctgt
    10861 ccattgtctg gaaccagaag ccgcccgcgc ctgaactgcc gccggacatc atcggaaaaa
    10921 cccgggcaaa gtacgaagaa gcctattccc gtctggtaaa ataaaatatc caaacccatt
    10981 gacagacgcc ataccgttat tacattacgc ggtgtttatc ttcgcgtcgt cgtgatttga
    11041 ggaggcatgc cttggctgga aaaaaactgc cggattaccg attaaaacag aaaattttgt
    11101 atatcgacaa aacttctcct gaatcgctca tcagttacgg ggacctgtac tgggaagcgg
    11161 gggcgccctc cgacgcgctg gacttttacg cacgggcgtc gcattcggcc ggcctgcaga
    11221 aaatccggga tgccgcgctg caaaacggcg atgtttttct gttccatggc gcggccagag
    11281 cgctggggct ggagcttcgg gacgaagact gggaaaacat cgcacggacg gccgcggcgc
    11341 ttcaaaaatt ctctttttcc gtacaggcct cgaaaaaaat cagcaacgcc gaacggacgg
    11401 acgcgctcgc ggcatggatc aaggcggagg aagcaaagca aagcgcatga ccaagaggga
    11461 ctactacgaa attctgggcg tcggcaaaaa cgccacggat gaagaactga aaaaatgcta
    11521 ccggaagatc gccatgcaat gtcatcccga ccggaacccg ggcgataagc aggcggaaga
    11581 gcggttcaag gaagcggccg aagcctacga agttttaagc gaccggcaaa agagggaaac
    11641 ctacgaccag ttcggtcatg cgggcctgag caacagcgga tttcagggtt tcagcggatt
    11701 tgacgatgtg ttttccggct tcggcgacat cttcgaggat gtcttcggat acggccgggg
    11761 ccgcggcggc aggcgaacgc gtccggccgc gcgggcgggc gccgatcttc gctatgatct
    11821 gaaaatttct tttctcgacg cggcgttcgg aatcaccacc gcgatcgaac ttgaaaagct
    11881 tcacacctgt catgaatgcg agggaacggg cgcggcggcg ggcacaaccc cttccacctg
    11941 ccccacctgc cacggcagag gccaggtggt tcagtcgagc ggctttttca ccatcagctc
    12001 cacctgcccc cactgcaacg gccacgggag gatcattgcc aagccctgcc atgcctgccg
    12061 gggtacgggc caggagaagc ggcgcaaaac cgtcgaactg aaaattcccg ccggcgtgga
    12121 aaccggttca cgcctgcgtc tgcgcggaga aggcgaagcg ggagaactgg gaggccccag
    12181 cggagacctg tacgtctttc tgcacgtgga agaccacgaa ttcttcttgc gctcggaaga
    12241 cgatatttcg tgccgcatcc cgatctcgat cgtgcaggcg gctctcggcg cgacaattga
    12301 agtgcccacg ctgaaagaca cggaaaaaat taaaattccc aggggtacgc aaaccggcca
    12361 gaccttccgt ctgaaaggca aggggatccc gcatctccag ggatacggcc gcggagacca
    12421 gatcatcgaa gtcttcgtcc gaatcccgac ggagctgacc cgaaagcagg aagatctcct
    12481 gcgcgaattt gaaaaaagtt ctcgacaata aagttgcaat ccattccaca atatgtttac
    12541 aatgccgcaa cttgaatggc ccggaggaat gcgcgatgaa aagcaagata ggtcgtctcg
    12601 tggtttgcat gtttgccctg atgtcccttg cgggatgcgg ggctctggtt gccggcggag
    12661 cggtggtcgg cgcgggcgcg ggtacgtatt tgtacgccaa cggcgagctg aagacggact
    12721 acgcggcctc gttcgacacc gtctggagcg cctgtgaaaa aacggtcgcg gagatgcgcg
    12781 gaatacaggt tcagcccacc agggaaattg ccaagggaac catttcggcc gtcatcaatg
    12841 atgaaaacgt gaagattgac attgcctatc gggcaaaaga acagacgacg gtcggcgtcc
    12901 gtgtgggcct gatcggcaac aagctgtctt cccaggtgat ccacgataaa atagccgatc
    12961 agctggccaa acgataatgt tttgcgacgc cccgtcgggg ccggcgctgg catcttcatg
    13021 aacaagaaat ttcttttcgg catcctgctg ggcgtggtcc tgatttactt ttccattcgg
    13081 ggcattcact tcaacgacac gcttgcttcc ctgaagaaaa tccatctcgg ctgggcggcg
    13141 ttgtcgcttt tcttcatcgt attcatgcag gccctgcgat cctaccgctg gggcgtcatc
    13201 ctcgaaccga tggaaaaaat ttcccaggga acgctctttg ccgtcaccag cgtcggattc
    13261 ctggcgattg ccgcgatacc ggcccggatc ggggagctgg cccggcccta tctcatctcg
    13321 aaaaaaagcg gcgtcaaaat gtcatcggcg ctgggcacca tcgtcgtgga gcgcgtcctc
    13381 gacagcctgg ccgtgctgac catcaccatc gcggttctca ttgcgcagca tgacctgccg
    13441 cgctggatgt ttacctccgg gattcttttc tttcttctga ccctgatgat gatcgttttc
    13501 atcgtggggc tggtctggcg gcgggaaacc gccgtttccg tcatcgactg gatcctgcgg
    13561 cggctgcccg gcgctttggc gcaaaaagtc aatcccctca tccatcattt catcgacggc
    13621 ttccaggtca tcaccgacgt caaacggctg ctgtccctgc tggccttatc cgccgtggtc
    13681 tggctggtgg acgtcgccgc gatttacacg ctgctcatgg ccttcggcct taatctgccg
    13741 gtgctggcgg ctttcgtggt tatggtgatt ttgatcgcgg gcatcgccat tcccaccgcg
    13801 ccgggtttta tcggcaactg gcactacgcc tgcatcctgg ggctgagcct gttcgggatt
    13861 gccaaacccc aaggcctgtc cttcgcgctg gtctatcatt tcctgtcgat gatggtcgtg
    13921 gtcgttctgg gtgcggcgtt tctgccgttc aataaatttt cgctttcgga aatgacgaag
    13981 cagatgaaga agactgaaga ctgaagactg aagactgaag actgaaggct gaaggctgaa
    14041 ggctgaaggt tacaaggatg tgcttcgcac ttgatttgga ataaagaaca taaagggaca
    14101 aggcattgca atcaatgtct tgccctttgc attttgacct tcagtcttca gcctattctt
    14161 ttaccggcac tttcagggct ttgagttttt tgtgcagatt actgcgctcc aggccgatgg
    14221 cttcggcggt tttggagata ttgccgtcgt attcttccag ctttttcaaa ataaactgcc
    14281 gctcgaaatc gagccgggca tcccggagcg aatcaccggc cgccgcggtc tcttcgaaag
    14341 aaggcgcgtc catgtcggct ttgacgctga gttcgggaat atcttccgcc cgtatctcat
    14401 tggacggcgt cagaatgatc agccgttcga tgatattttt cagctcgcgg acgttgccgg
    14461 gccagtcatg caccattaat ttttccaggg cggcggcggt cagggtcttc agcggctgcc
    14521 cctctttggc ggcaaagtcc aggagaaacc gctccgtcag gtgaatgatg tcttctttcc
    14581 ggtcgcgcaa cggcggcacg cgcaggggaa tgacgtgcag gcggtagtac agatcctgcc
    14641 ggaaccggcc cgcctccatt tccttctcca ggtccttgtt cgtcgcagcc agaactctta
    14701 catccatgtc gatcagcttg ttgccgccca ctctttcaaa ttttttctcc tggagaatcc
    14761 gcagaatctt ggcctgggcc ttcaggctca tgtccgccac ttcatccaga aaaatcgtcc
    14821 cctcgtgggc cagatcaaac ttgccgcgct tcttctccgt cgctccggtg aaagcgccct
    14881 tttcgtgccc gaacagctcg ctctcgatga gctcctcggg aatggccgcg cagttgactt
    14941 ccacgatcgg cttgtgggag cggcggctca ggtgatggat ggagcgcgcc accagctcct
    15001 ttcccgtccc gttttcgccc atgatcagaa tccacgcgtt ggtcggcgcg acgatgccga
    15061 tcatttcctt gagttcaacg atgaccggac tggtcccggt cagttcgtac tggtgcgaca
    15121 ctttctgttt gagcagaatg ttttcttctt ccaggcggac aaggttcaac gcgttgttca
    15181 ccaggatcat cactttgtcc agcgacagcg gtttttcaat aaa
//
LOCUS       DAOSIM010000023        15182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_4042_length_15182_cov_9.374562, whole genome shotgun sequence.
ACCESSION   DAOSIM010000023 DAOSIM010000000
VERSION     DAOSIM010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 15182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15182
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4042_length_15182_cov_9.374562"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..1242
                     /locus_tag="PLR47_02125"
     CDS             256..1242
                     /locus_tag="PLR47_02125"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOG11762.1"
                     /translation="MSDETKRSEPVESVDVNEDEIDLLELARTIWKGRKLIIWIVCAF
                     TLATIILSLFMTNIYTARAVIKPSSQSQSGGRLASLASQFGGLASLAGIAMPSSASST
                     EIVALLESNILKKEVIETHQLLPVLFKDQWDENTKSWKKPGVSLNPLAFIAKLRPAPK
                     TVKKEPGVPGTWDGIRVLNGIVKINYDMKEDIITISVDFPDPDMAARIANYFVLTLND
                     HMSMEAKRIATTNREYLEQQLTQTSDPIVQQKIYNLIADKIETTMMAEVKEGFAFKVL
                     DPPMAPDKKSKPKRAQMAVVAFMVSVFIGIFAVLFKEYIRKIKENSAGGHHA"
     gene            1235..4714
                     /locus_tag="PLR47_02130"
     CDS             1235..4714
                     /locus_tag="PLR47_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOG11763.1"
                     /translation="MRSQRFFTFLVLCLFCIAVGLAGAGIGRAQTAGSSSLSLNDQRA
                     IQSSLQKAGINLTPEEIRKGKEAFEKQEGVRTGKMYKMPNAPEEQQMQRPEGYLDSRT
                     AVEAPKEVSVFNRIRKIGEYQDVLTDLKPFGYDFFHGTEVKVLTERQDIPVPLDYMIG
                     PGDEVNILLWGRMSAQLTLVVDRDGKITIPDIGPVQVAGMTFGEMSKHLIAKAEQIVG
                     TNIDISMGSTRTIPVFVLGDVERPGTYTIGALATITDALMLARGPSAIGSMRRVELRR
                     KNRVVTKFDLYDLFLKGDKSKDVVLQAGDVVFVPVTGPLVGITGNVKRPAIYELKDRF
                     DLQHLFELAGGIIPSAYTQQMQVERVVKNEKQIVIDINDKTLAKVRDFVLQDADLIKV
                     FSIMDVDANAVYLRGNVKRPGKYSFAPGMKIRDLINQPEDLQSEAHMDYALIKRENPP
                     NREAVLIPFNLGKLILQNDEAYNYDLAPKDQIFVFHQSLFKDQPFVTLEGEVRGFEPT
                     AQEKALRAGQYAQAAPGPYGQAVPGQFGAPAVPGSVQYGQPASGTYSAPAPAQPGTAA
                     PGQYNVAAPRQPAVSPSGQYGQPTAGSYGSQVSGQPGVALPGQYGQTAPAPYGVQVPG
                     QPGAAFPDQYYPYNENLFEIRQIKDTLRFDYEKDKAREKDEIFYLLMAKIEEIEAEIE
                     KDNRVTPGIINQLQIELEKAGRKDLSLRLGKLDEKLKVAIRIDLVGNMKVKDAILNAG
                     GLTANASMESGEIIRQYPNNEFRTTYFNVARAMEGDPRDNLLLQDKDRIVIHSILEKN
                     PRKNIFVTGDVTKPGTYQFTENMTVRDLIFKAGNVLESAYLDEAEITSVSVVNGRLGD
                     LSHRKVNLKKALEGDAGHNVTLLPNDRLHVKQIADYHNVRFITLSGQMAFPGKYPFRK
                     GERLSDIVERAGGFTPHAYLRGAQFTRVRVKELQQQGLKEMADRMERELLSGGAEQFA
                     TAVSKEEIAAKQGELEQKKKFVDTLRKTRATGRMTVYVADAGKLRNTEYDFELEDGDT
                     LYVPERNSVVNVVGSVMSQSSHLYSENLSHQDYIDASGGYSYYADASNVYILKVDGGA
                     RRLSKNFIGWNSSRSRWEMTAYGAGVKDIEPGDTIVVPEKIERIAWLREIKDITQILM
                     NIAVVAGVTIKLF"
     assembly_gap    5080..5179
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5546..6283
                     /locus_tag="PLR47_02135"
     CDS             5546..6283
                     /locus_tag="PLR47_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG11764.1"
                     /translation="MVDRKFKKLSIVVPVFNEERYLEAVIGKVVAQPLPGGLEKELIL
                     INDASQDNSWNIMQALPEKFPSVTMQLINKTVNEGKGAALRDGFAKITGDIVIVQDAD
                     FEYDPADYPKLLQPILDDKADVVYGSRFIGEPHRVLYFWHQVANNILTTISNMLTNLN
                     LTDMEVCYKVFIREVADRLKLKSPRFGVEPEITAKIARMRLNGRHLRVYETGISYAGR
                     TYEEGKKINWKDAVSAIFQIIRFRFMD"
     gene            6564..9374
                     /gene="ppdK"
                     /locus_tag="PLR47_02140"
     CDS             6564..9374
                     /gene="ppdK"
                     /locus_tag="PLR47_02140"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOG11765.1"
                     /translation="MAKYVYSFSEGYGKSKNLLGGKGVGLAEMAHLKIPIPPGFTITT
                     EVCMEYLRNKGRYPQGLKEQVNKALDKMEKETGMRFGDPKNPLLCSARSGARSSMPGM
                     METVLNIGLTSKTIAGMIEKTNNPRFVLDSYRRLIQMYSDVVMEKAEGIEPEEGMGVR
                     QQLEREMAAMKERRNVKLDVDLTDKDLAQLAEIYKAKVKEVLGKPFPDDAMEQLWGAI
                     GAVFKSWNGKRAVSYRRIEGIPDDWGTAVNVQAMVFGNMGENSATGVAFTRNPATGEN
                     KFYGEWLPNAQGEDVVAGIRTPNPINEETRQSDDVDGHPSLEKAMPKAYTEIKAIRNK
                     LEAHFRDMQDLEFTIQDGRVWMLQTRTGKRNGPAAVRMAVEMYKSKMISAEEAISRVT
                     PSQLDEMLHPMVDPKAEATAEFLVKGLPAGPGGAVGQVAFTSDVAMEWRKEGKKVILV
                     REETNPEDVEGMRAAEGVLTARGGMTSHAALVARGWGKCCIVGAGTLHVDIAQKIMKV
                     DGKAVREGDFVTLNGTKGRAYIGSLPMISGAENDVNFAAFMKLCDSLRRLKIRTNAET
                     PKDAAKAVQFGAEGIGLFRTEHMFYGENSEQPLFYLRKMIIADTEEKRRAALDELFPF
                     VKGFIKKTIEAMGGRPVTIRLLDPPLHEFVPENPEERARLAESLGISVEKLNGRAEGL
                     HETNPMMGHRGVRLGISYPEVSEMQIRAILEGSAELIKEGKKALPEIMIPVVCDVAEF
                     SNQAALVKKVYPEVLAKYGLKKMSYMVGTMIEIPRAALLADKIAEEAEFFSFGTNDLT
                     QMTYGFSRDDVGAFVPGYLAKKIIPVDPFQILDQTGVGQLIQMGVERGRSARPGLKVG
                     ICGEHGGEPSSVMFCHRVGMDYVSCSPYRIPLARLAAAQAAVEEKMSSKPKTKAKAKA
                     KTKAKTTVKAKAKVKKAKKSRK"
     gene            9440..11227
                     /locus_tag="PLR47_02145"
     CDS             9440..11227
                     /locus_tag="PLR47_02145"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOG11766.1"
                     /translation="MELAVKNKKLLSWVKEMKQLCAPKKVYWCDGSKKEYNTLMAQMV
                     KSGAAIKLKKRKNCFLFRSDPSDVARVENRTYISYPDQVDAGPTNNWVAPDALKKTMK
                     NLYKGCMKGRTMYIIPFSMGPINSPIAKIGIEITDSPYVVCNMHTMTRVGKKVLKKLG
                     DTGEFIPCMHSIGAPLEKGQKDVAWPCAPIEKKYISHFPDENLIWSYGSGYGGNALLG
                     KKCLALRIASAMAKREGWMAEHMLILRLTNPKGKQYHIAAAFPSACGKTNLAMLQPTI
                     KGWTCECIGDDIAWMKIGPDGRLYAINPEAGFFGVAPGTSYDSNPVAMDTIKKNVIFT
                     NCALTDNGDVWWEGMDGKPPKHAIDWKGRDWPNDKNEPAAHPNARFTAPAAQCPMICP
                     DWEKPEGVPIDIFIFGGRRAGVVPLVNEAFSFDHGVFLGATASSETTAANIGAVGNLR
                     RDPFAMQPFCGYNMGDYFQHWLDMGDDLGDKAPKIFYVNWFRKTADGRWLWPGFGENS
                     RVLKWMCERVDGKAEAVQTPIGLLPRLGGLDLKGLKIDERDIQELLRVDLDAWKAEIP
                     DIEKHFAQFGDRLPARLRKQFAELKKRLG"
     gene            complement(11342..>12691)
                     /locus_tag="PLR47_02150"
     CDS             complement(11342..>12691)
                     /locus_tag="PLR47_02150"
                     /inference="COORDINATES: protein motif:HMM:NF015831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HOG11767.1"
                     /translation="SGLATLNSAQVTNNLTVNGNTTLGNAITDATTVNGTLSVSGATT
                     THGITNTGNITNSGDISTATLHTSGLATLNSAQINTTLNVDGATTTNGITNTGDIATD
                     TLQTSGLATLNSAQVNTTLNVDGATTLGSTLDVAGAATLNDTLNVAGDTTLASNLAVG
                     GNTTLAGSLDVAGATTTNGITNTGNITTDTLNTTGDTNVGGNLFVSGATTLTGDLVAN
                     GTNNTIGTAGVSTNTITGVDNNIIATGNNFITGDMNTITATTGNTIGVTGGSTITTTA
                     DQVTALTADGSGFVANNGGNVTVLNTTGHGLIVSPTETVVSGGTTSTTLTLNNTGATF
                     RNTVTGGPARVSGVADGVNDNDAVNVRQYNVLKDGVEKANMGIASVSALSAIPAALPG
                     KRVALGAGYGYFEDESAVAIGVKARVLENMSVSAGIGVGISDTSSTYTANAGFSFSF"
     assembly_gap    12692..12791
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12792..14590)
                     /locus_tag="PLR47_02155"
     CDS             complement(<12792..14590)
                     /locus_tag="PLR47_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11768.1"
                     /translation="MKNRFLMILALLFVGLFLSGGFLAGKVMADVVGAYVDSGGISSD
                     IDEPRGGYMFVATPGDTGTSTNVSGYYINAYFAGEVTQDPGANLFSIDYTYDPATFSY
                     TFGNAATLSLDSGSNAYLSYWDSINRIENGLLADATGISVGSIDYNSGLHYGLTVDRS
                     GSFIDGPFFVDTNGVDTPDVAGTGVLYLDDGSMLIGTTNLANNNWNGLSADMTSATMT
                     GGTGTTTWTLSNSGAVLNDSTNGDIFTVGSYGDTTVAGTLTTGDVIYAGGNLNMGNHD
                     INNVRDINNVRDFSVTRNTTLGNGSTDSTTVTGLFNVNGDSSLRSTSGLFGMDINDSL
                     AQLGYDDGVTKRNGFMADSGRAVANSLDYTTGYQATLSLDSTDKAFISFNDGTVESGL
                     AAGPAGLMVGSTSGSTNMGMTVDTFTNDMIVGYMDTSSSEAYGLHANNSEVNLGYNNA
                     GSFRGVTVNNSLISAYSPDATGAAGMGLGQISIPMGPSMSGAYIGYDNGTNYNGLVSA
                     GIGTAVGSINYATGEISGLAADGTNLMVGYLNGTTGATNGLMANSTEIVVNSFDSMTS
                     NEATLSLNSTDKAYLSFWDDANSIENGLLATIS"
     gene            complement(14684..>15182)
                     /locus_tag="PLR47_02160"
     CDS             complement(14684..>15182)
                     /locus_tag="PLR47_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11769.1"
                     /translation="PVSAETSNTPALSKNRATPQPAAQKANTITQAALKNGVRTCAGR
                     INQVTNFLTAGAKGGGFIMIYPTSRPDRHMTSVSMEIPLQDNTAYASASFAPGQENYC
                     EGLYETVVYWPQTCSEVAEKQFGKFKRTGALSRHIYVRDGGATKVFLMPAGAGCVSIK
                     KELIR"
BASE COUNT         3723 a         3657 c         4115 g         3487 t
ORIGIN      
        1 tattgattcc tggttcccgg cctccgccgg gaagacgagg aggtactgga ttccggctaa
       61 gcccacgccg gaatgacgag cggttctgaa tttcagttgc ttacggcatg acttcatgtt
      121 atgcaaagaa gcctttgcgt aattgatttt tatgactttt tgagcaacaa tttcgccgat
      181 ccaaaatttg ccgggattgg caggggcgag aaatctttct ttttaaaata accggaatat
      241 tcaggaaagg tccatatgag cgacgaaaca aaaagatcag aacccgtcga gagcgttgat
      301 gtaaatgaag atgaaatcga tcttctggaa ctggcccgaa caatctggaa ggggcgaaag
      361 ttgatcatct ggattgtctg cgccttcacc ctggcgacga tcattttatc tctttttatg
      421 acaaatattt acacggcccg ggcggtgatc aagccgtcat cccagtcgca aagcggcggg
      481 agacttgcct cgctggccag ccagttcggc ggcctggcca gcctggccgg aattgccatg
      541 ccgtcttcgg catcctctac ggaaattgtc gctcttctgg agtccaatat tttgaaaaaa
      601 gaggtgattg aaacccatca actgctgccg gtattgttta aagaccagtg ggatgaaaat
      661 acgaaatcat ggaaaaagcc gggtgtcagt cttaaccctc tggccttcat tgccaaatta
      721 aggccggcgc caaaaaccgt taaaaaagaa ccgggagttc ccggtacatg ggacggcatc
      781 cgtgttttga atggtatcgt caaaatcaat tatgatatga aggaagacat cattaccatc
      841 tccgtcgatt ttcctgatcc ggacatggcc gcccgcatcg ccaactattt tgttcttaca
      901 ttgaatgacc atatgagcat ggaagcaaag aggattgcca cgacgaaccg ggaatatctg
      961 gagcagcaac tgacccaaac cagcgatccc attgttcaac aaaaaattta caacctcatc
     1021 gccgataaaa ttgaaaccac gatgatggcg gaagtcaaag aagggtttgc gttcaaggtg
     1081 ttggatccgc cgatggcgcc cgacaaaaag agcaaaccca aacgggcgca gatggccgtt
     1141 gtcgctttta tggtgtcggt gtttatcggt atttttgcgg tattgttcaa agaatacata
     1201 agaaaaatca aagaaaattc tgccggaggg catcatgcgt agtcaacgat tttttacatt
     1261 ccttgtgctg tgtttattct gtatcgcagt cggtcttgcg ggggcgggca ttggccgggc
     1321 acagacggcc ggttcatcgt ctctgagtct caatgatcag cgggccattc aatccagtct
     1381 gcagaaagcc ggcatcaatc tgacaccgga agagatccgc aagggcaagg aagcctttga
     1441 aaaacaggaa ggtgtccgga cgggcaaaat gtataaaatg cccaacgcgc ctgaagagca
     1501 gcagatgcag cggccggaag gctacctgga cagccggacg gcggttgaag cgcccaaaga
     1561 agtatcggta ttcaaccgga taagaaagat cggcgaatat caggatgtgt taaccgatct
     1621 gaagcctttc ggttacgatt tttttcacgg tacggaagtc aaggtgttaa cggaacgcca
     1681 ggatattccg gttcctctgg attatatgat cgggccgggc gatgaagtga atatcctttt
     1741 atgggggcgg atgagcgccc aactcacgct cgtggtggac cgggacggca aaatcacgat
     1801 tcccgatatc ggtcccgtgc aggtggcggg catgaccttc ggtgaaatgt ccaaacacct
     1861 cattgcgaaa gccgaacaga ttgtaggaac gaatattgat atttccatgg ggtccacccg
     1921 gacgattccc gtttttgttc tgggcgatgt tgaacgtccc ggcacgtata cgatcggcgc
     1981 attggcaacc attaccgatg cgctcatgct ggcgcgcggt ccttcggcca tcggctcgat
     2041 gagacgcgtg gaactgcgca gaaaaaacag ggtcgtgacc aaattcgacc tgtatgatct
     2101 tttcctgaaa ggcgacaagt ccaaagatgt cgtcctgcag gcgggcgatg tggtttttgt
     2161 acccgtcacc ggtccgctgg tcggcattac cggcaacgtc aaacggccgg cgatttatga
     2221 actcaaagac agattcgact tgcagcatct ttttgaactg gccggcggca tcattcccag
     2281 cgcctacacg cagcagatgc aggtggaacg ggtcgttaaa aatgaaaaac agattgtcat
     2341 cgatatcaac gataagacgc tcgccaaggt gagggatttc gtgcttcagg acgcggatct
     2401 gatcaaggtg ttttccatta tggatgtgga cgccaacgcc gtttatctga ggggcaatgt
     2461 caaacgtccg gggaaatatt cctttgcgcc cgggatgaag atccgggatc tgatcaacca
     2521 gccggaggat ctgcagagcg aagctcacat ggattacgcg ctgatcaaac gggaaaaccc
     2581 gcccaaccgg gaagctgttc tgattccgtt taatctgggc aaactcattt tgcaaaatga
     2641 cgaagcgtac aattatgacc tggcgcccaa agatcagatt tttgtctttc accagtcgtt
     2701 attcaaggat cagcctttcg tcacgctgga aggagaagtt cgcgggttcg aacctacggc
     2761 tcaggaaaaa gctctgagag cgggccaata tgcacaggcc gcacccggtc cctacggcca
     2821 ggccgttccg ggccagttcg gcgctccggc cgttcccggt tccgtccagt acggtcaacc
     2881 tgcttccggt acctacagcg ctccggcgcc cgcgcagccg ggtactgcgg cgcccggtca
     2941 gtacaatgtc gcagcgcccc gtcagcctgc agtttcgcct tcgggccaat atggacagcc
     3001 gacggccggt tcgtacggct cccaggtttc cggtcagccc ggggttgcat tgcccggtca
     3061 atacggccag actgcccccg ctccgtatgg tgttcaggtt cccggtcagc ccggggctgc
     3121 attccccgat cagtattacc cgtataacga aaatctgttc gagatcaggc aaattaaaga
     3181 cacattgcgt ttcgattacg aaaaggacaa agccagggaa aaggatgaaa ttttctatct
     3241 tttaatggcc aaaatagaag aaattgaagc ggagatcgaa aaagataaca gggtaacgcc
     3301 gggtatcatt aatcaactgc aaattgaact ggagaaagcg ggcagaaaag atctgtcgtt
     3361 gaggctcggc aaactggatg agaagttgaa agtggcaatc cggattgatc tggtcggcaa
     3421 catgaaggtg aaagacgcca ttttgaatgc cggcggcctg accgcaaatg ccagcatgga
     3481 aagcggcgaa attatccgcc agtatccgaa caatgaattc agaacaacgt atttcaacgt
     3541 tgccagagcg atggaaggcg atccgagaga taatcttctg ctgcaggata aagacaggat
     3601 cgtcattcat tcgatcctgg aaaagaatcc aaggaaaaat atttttgtca cgggcgatgt
     3661 caccaaaccg ggaacgtatc agtttacgga aaacatgact gtgcgcgatc tgattttcaa
     3721 ggcaggcaat gttctggagt ccgcgtatct tgatgaagcg gaaattacat cggtcagcgt
     3781 tgtgaacgga agactgggcg atctttccca caggaaagtc aatctgaaaa aagcgctgga
     3841 aggcgacgcc gggcacaatg tgacgctgct gcccaacgac cggctgcatg tcaaacagat
     3901 tgccgattat cacaatgtgc ggtttatcac gttatccggc cagatggctt tccccggcaa
     3961 gtatccgttc agaaaaggcg aaagattatc cgatatcgtt gaacgggccg gcggttttac
     4021 accgcatgcg tatttgcgcg gcgctcagtt cacgcgggtg cgggtgaaag agcttcagca
     4081 gcagggtctg aaagaaatgg ccgaccgcat ggagcgtgaa ttattatccg gcggggcgga
     4141 acagtttgcc accgctgttt caaaagagga aattgccgcc aaacagggcg agttggagca
     4201 aaagaaaaaa tttgtcgaca cgctgcgcaa gaccagggcc accggccgca tgacggttta
     4261 tgtggcggat gccggaaaac tccgaaacac ggagtatgat tttgagctgg aagacggcga
     4321 cacgctgtat gttccggaaa gaaacagcgt ggtgaatgtg gtcggctccg tgatgtcgca
     4381 gagcagccat ctgtattccg aaaatctcag ccatcaggat tacatcgatg cgtccggcgg
     4441 atactcctat tatgcggatg cgtccaatgt ctacatcctc aaagtagacg gcggcgcccg
     4501 acggctttcc aagaatttca tcggttggaa ttcttcccgc tcgcgctggg aaatgacggc
     4561 gtacggcgcc ggcgtcaagg atatcgaacc gggcgatacc attgttgttc cggaaaagat
     4621 tgaaaggatt gcctggctgc gggaaatcaa ggatattacg cagatcctga tgaatattgc
     4681 cgtagtcgcc ggtgtcacga tcaaactgtt ctaatgacac cgagttggga cgttaaaatt
     4741 agggttgtca cttcgagcga atgcgagaag tcttgaaaaa ggtttgagac tttgcaaaac
     4801 cacgtcaatt cgtcaatttt gtcataccgg cgcaggccgg tatccagaaa atggatcccc
     4861 gataggacca ctcggggatg acagaggatg gatctaacca gagggcgcca tggcctgtct
     4921 ccacgggaat ggcccaaaga aaaagttatg caaagctctc agtttgtaac tgtgaaatga
     4981 aagcattaag attcctcttc actggcgctc atcggaatga cgcagagttg ggacgttaaa
     5041 attagggttg tcacttcgag cgaatgcgag aagtcttgan nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnng agttgggacg ttaaaattag ggttgtcact tcgagcgaat
     5221 gcgagaagtc ttgaaaaagg tttgagactt tgcaaaacca cgtcaattcg tcaattttgt
     5281 cataccggcg caggccggta tccagaaaat ggatccccga taggaccact cggggatgac
     5341 agaggatgga tctaaccaga gggcgccatg gcctgtctcc acgggaatgg cccaaagaaa
     5401 aagttatgca aagctctcag tttgtaactg tgaaatgaaa gcattaagat tcctattcgc
     5461 ttcggcttat cggaagcgac aagtttctac aggattgtag atttttcgtt tctcgcaacc
     5521 agtgaatgcg ggggaaagtc gccggatggt cgatcgtaaa tttaaaaaac tttcaatagt
     5581 tgttccggtt ttcaacgaag agcgttatct ggaagcggtc atcggcaagg tcgtcgcgca
     5641 accgctgccc ggcggtctcg aaaaagaatt gattctgatc aacgacgcct cgcaggataa
     5701 ttcctggaat atcatgcagg cgctgccgga gaaatttcct tccgttacaa tgcagttgat
     5761 aaacaagacg gttaatgaag gcaagggcgc ggccctccgg gacggctttg ctaaaatcac
     5821 cggggatatc gtcattgttc aggatgccga tttcgagtat gatcccgccg attatcccaa
     5881 attgttgcag ccgatcctgg atgacaaagc ggatgtggtg tacggcagcc gcttcatcgg
     5941 cgagcctcac cgcgtgttgt atttctggca tcaggtggcc aacaatatcc tgacaactat
     6001 ttccaatatg ctgaccaatc ttaacctgac ggacatggag gtctgttaca aggtttttat
     6061 ccgcgaggtt gcggatcgtc tgaaactgaa aagcccccgt ttcggcgttg agcctgaaat
     6121 tacggccaaa atcgcccgta tgcgcttaaa cggcaggcat ttgcgggtat acgagaccgg
     6181 tatttcctat gccggccgca cctacgagga agggaaaaaa ataaactgga aggatgcggt
     6241 atcggccatt ttccagatta tccgtttccg gttcatggat taggtatttt tgacgccgag
     6301 ttatcacggc cgcttcccgt agccagcctc ttggtccggg aggtttttct tttccaaatc
     6361 atttcaataa catcaaattt tgtgttgcgg ctgcttcatt ttccaggccg gtcaatttca
     6421 gcatgatatg aatatgattg attatttaaa tagagcgatt cgcgtcgatt ttctacaatt
     6481 ttatcttgac aatgagcaca atcttatgac gatataacgc attaaaattt attggattaa
     6541 caaggaggtt gagaagcaac gttatggcta aatatgtata ctctttctcg gaaggatacg
     6601 gcaaaagcaa gaatcttttg ggcggcaagg gcgtcggcct tgcagagatg gctcatctta
     6661 aaattcctat ccccccgggt tttacaatta caacagaagt ttgcatggaa tatttgagaa
     6721 ataaaggccg ttatccgcag ggattgaaag aacaggtcaa caaagctctg gacaaaatgg
     6781 aaaaagaaac gggcatgaga ttcggcgatc cgaaaaatcc tctcttgtgt tcagcccgtt
     6841 cgggagcgcg ctccagcatg cccggcatga tggagactgt gctcaatatc ggtctgacgt
     6901 caaagacgat tgccggcatg atcgaaaaaa ccaacaatcc ccgtttcgtt ctggattcgt
     6961 accgtcgtct gattcagatg tattcggatg tcgtgatgga aaaagccgaa ggaatcgagc
     7021 cggaagaggg aatgggcgtc cgtcagcagc tggaaaggga aatggctgcg atgaaagaac
     7081 gccggaatgt caagctggat gtcgatttga cggacaaaga tctggcccaa ctggctgaaa
     7141 tttacaaggc caaagtcaag gaagtgctgg gcaaaccgtt tccggatgat gccatggaac
     7201 agttgtgggg cgcgatcggc gcggttttca agagctggaa cggcaagcgc gccgtttctt
     7261 accgccggat agaaggcatt cccgatgact ggggcacagc agtcaacgtg caggcgatgg
     7321 ttttcggcaa catgggcgaa aattccgcca ccggtgttgc gtttacccgc aatccggcca
     7381 ccggcgaaaa caaattctac ggcgaatggc tgcccaatgc gcagggtgaa gacgttgtcg
     7441 cgggaatccg aacccccaat ccgatcaacg aggaaacccg ccagtcggat gatgtcgacg
     7501 gccatccttc tctggaaaaa gccatgccca aggcttatac cgaaatcaag gcgatccgca
     7561 acaaactgga agcgcacttc cgtgatatgc aggacctgga gttcaccatt caggacggcc
     7621 gcgtctggat gctgcagacc cgtaccggaa agcgcaacgg tcccgccgcg gtgcgcatgg
     7681 ccgtggaaat gtacaaaagc aaaatgatca gcgcagaaga agcgatttcc cgtgttactc
     7741 cgtcacagct tgatgaaatg ctgcatccga tggttgatcc gaaagccgaa gccacagcgg
     7801 aatttctggt caagggtctt ccggccggtc ccggcggcgc cgtaggccag gttgcgttta
     7861 cctccgacgt ggcgatggaa tggaggaagg aaggcaaaaa agtcattctg gttcgcgaag
     7921 aaacgaatcc cgaagatgtg gaaggcatgc gcgcggctga aggcgtgctt acggcccgcg
     7981 gcggcatgac gtcgcatgcg gcgctggttg cccgcggctg gggcaagtgc tgtatcgtcg
     8041 gcgccggcac gctgcatgtg gacattgcgc aaaagatcat gaaggtggac ggcaaggcgg
     8101 tgcgcgaagg cgattttgtc acgctcaatg gaaccaaggg acgcgcttat attggctcgc
     8161 tgccgatgat cagcggcgcg gaaaatgatg tcaattttgc cgcttttatg aagctgtgcg
     8221 attcattacg gcgtttgaag atccgcacca atgcggaaac gccgaaagac gccgccaagg
     8281 ccgtacagtt cggcgccgaa ggcatcggac tgttccgcac ggaacacatg ttctacggcg
     8341 agaacagcga acagccgctg ttctatctgc gcaaaatgat cattgcggac acggaggaaa
     8401 aacgccgcgc ggcgctggat gaattgttcc cgttcgtcaa gggcttcatt aaaaagacca
     8461 tcgaagccat gggcggaaga ccggtcacca tccgcctgct ggatccgccc ctgcatgaat
     8521 ttgtgccgga gaatcctgag gaacgcgcgc gtctcgcgga gagcctcggc atttcggttg
     8581 agaaactcaa cggccgcgcc gaaggtctgc atgaaaccaa tccgatgatg ggccatcgcg
     8641 gcgtccgttt gggcatcagt taccctgaag tcagcgaaat gcagattcgc gccattctgg
     8701 aaggctcggc ggagctgatc aaggaaggca agaaagcgtt accggaaatc atgattccgg
     8761 ttgtctgcga tgtggccgaa ttcagcaatc aggcggctct ggtgaaaaag gtttatccgg
     8821 aagtgctggc caaatacggc cttaagaaaa tgtcctacat ggtggggacg atgatcgaga
     8881 ttccgcgcgc ggctctgctg gcggacaaga ttgccgaaga ggcggaattc ttcagtttcg
     8941 gaacgaacga tctgacgcag atgacgtacg gcttcagccg cgacgatgtc ggcgcgttcg
     9001 tgcccggtta tctggcgaag aaaatcattc ccgttgatcc gttccagatt ctggatcaga
     9061 ccggcgtcgg ccagttgatt cagatgggcg tggaaagagg tcgcagcgcg cgtcccggtc
     9121 tgaaggtcgg gatttgcggc gagcacggcg gcgaaccgtc gagtgtcatg ttctgccatc
     9181 gcgtgggcat ggattatgtc agctgttcgc cttaccgcat tccgctggcg cgtctggctg
     9241 ccgctcaggc tgcggtcgaa gagaaaatga gctcgaagcc aaaaaccaag gctaaagcta
     9301 aagccaaaac aaaggccaaa acgaccgtga aggcaaaagc gaaagtgaaa aaagcgaaaa
     9361 agtcacgcaa gtaattaacc atagagcgcc cggtgacaag atcgggcgct cagattaatt
     9421 caaaaaggaa aaatatttta tggaattagc tgtaaaaaac aaaaaattgc tgagttgggt
     9481 caaagaaatg aaacagctat gcgcacccaa aaaagtgtat tggtgcgatg gttccaaaaa
     9541 ggaatacaac acattgatgg cgcagatggt gaaaagcggt gcggcgatca agctgaagaa
     9601 gagaaaaaac tgttttctgt tccgttccga tccttcggat gtggcgcgtg tggagaaccg
     9661 cacgtatatc agctatcccg atcaggttga cgccggaccc accaataact gggttgctcc
     9721 cgatgcattg aagaaaacga tgaaaaatct ttacaaggga tgcatgaagg gacgcacgat
     9781 gtacatcatc cccttttcca tggggccgat aaattcgccg attgccaaaa tcggcatcga
     9841 aatcaccgac agtccctacg ttgtctgcaa tatgcacacg atgacccgcg tgggtaaaaa
     9901 ggttctcaaa aaactcggcg atacggggga gtttattccc tgcatgcatt ccatcggcgc
     9961 tccgctggaa aaaggacaga aggatgttgc ctggccctgt gcgccgattg agaaaaagta
    10021 tatcagccat ttcccggatg agaacctgat ctggtcgtac ggttccggct acggcggcaa
    10081 cgccctgctg ggcaagaagt gtctggcgct gcgcattgcg tcggcgatgg ccaaacgcga
    10141 gggttggatg gccgaacata tgctgattct gagactgacg aatcccaaag gcaaacagta
    10201 tcacattgcc gcggcttttc cttccgcatg cggcaagacc aatctggcga tgctgcagcc
    10261 gaccatcaaa ggctggacgt gcgaatgcat cggcgacgac attgcctgga tgaaaatcgg
    10321 tcccgatgga aggctctacg cgatcaatcc ggaagccgga ttcttcggcg tggcgccggg
    10381 aacatcctac gattccaatc ccgtggcgat ggataccatc aagaaaaacg ttatttttac
    10441 caactgtgcc ctgacggaca acggcgacgt ctggtgggaa ggcatggacg gcaagcctcc
    10501 caaacatgcc attgactgga aaggaaggga ctggcccaac gacaagaatg aaccggcggc
    10561 gcatcccaat gcgcggttta ccgcccccgc tgcgcagtgc ccgatgattt gtccggactg
    10621 ggaaaaaccg gaaggcgtgc cgattgatat ttttatcttc ggcggacggc gcgcaggcgt
    10681 tgtgcctctg gtgaatgaag ctttcagttt cgatcacggt gtctttctgg gcgcgacggc
    10741 ttcgtcggaa accacggcag ccaacatcgg agcggtcggt aatctgcggc gcgatccttt
    10801 cgcgatgcag ccgttctgcg gctacaacat gggcgactat ttccagcact ggctggatat
    10861 gggcgatgac cttggcgaca aggctccgaa aattttctat gtgaactggt tcaggaaaac
    10921 agcggatggc cgctggctct ggccgggctt cggtgaaaac agccgtgtcc tgaaatggat
    10981 gtgcgagcgc gtggacggca aggccgaagc cgttcaaact ccgatcggac tgcttccccg
    11041 gcttggcggt ctggatttga aaggcctgaa aattgatgag cgggatattc aggaattgct
    11101 ccgcgtcgat ctcgatgcct ggaaggcgga aattcccgac atcgaaaaac acttcgcgca
    11161 gtttggagac agattgcccg caagactccg gaaacagttt gcagaactga aaaaaagact
    11221 gggatagttt acggcaaaaa caggtcaacc gtttgacatt tacataaacg gctcacgcaa
    11281 ttaagaaggg gacagcgaat aatcgctgtc ccctcttttt gtttaagatt gctctttgag
    11341 attagaagct gaaagagaat ccggcatttg ccgtataggt cgaactggtg tcgcttatgc
    11401 cgacgccgat gcctgcggaa acgctcatat tttccagaac tctggccttg acgccgatgg
    11461 cgacggcgct ttcatcttca aagtacccgt aacctgcgcc cagagccact cgctttccgg
    11521 gcaatgcggc ggggatggct gaaagagccg atacggaagc aatacccata ttggcttttt
    11581 ccacaccgtc cttgagcacg ttgtactggc gaacgttgac cgcatcgtta tcattgacac
    11641 cgtccgccac tcccgaaaca cgcgcaggac cgccggttac cgtatttctg aaggtggctc
    11701 ccgtgttgtt caacgtcagc gttgtggagg tggttccgcc ggaaacaact gtttccgtcg
    11761 gagaaacgat caacccgtgt cccgttgtgt tcagcacggt cacattgccg ccgttgttgg
    11821 caacgaatcc gctgccgtca gcggtcagtg cggttacctg atccgccgtc gtggtgatgg
    11881 tcgacccgcc ggtaacgccg atggtgttgc cggttgtcgc cgtgatggtg ttcatgtcgc
    11941 cggttatgaa attgttgccc gtggcgatga tgttattgtc aacgccggtg atcgtattgg
    12001 tcgaaacacc cgcggtcccg atggtgttgt tcgtcccgtt ggcaaccaga tcgccggtta
    12061 aggttgtggc gcccgagaca aacagattgc cgccgacatt cgtatcgccg gtggtattga
    12121 gcgtatcggt tgttatgttg ccggtattgg taatgccgtt ggtggttgtc gctcctgcaa
    12181 catccagaga tccggccagt gttgtatttc ctccgacagc cagattggat gcgagggtcg
    12241 tatcgcccgc aacgttcagc gtgtcgttga gtgttgcggc acccgcgaca tcaagcgtgc
    12301 tgcccagcgt ggtcgctccg tcgacgttca gggtcgtgtt gacctgtgcc gaattgaggg
    12361 tcgccagacc ggatgtctgg agagtatccg ttgcaatgtc gccggtattg gtgatgccgt
    12421 tggtagtcgt ggcgccgtcg acattgagag tcgtgttgat ttgcgccgaa ttgagggtcg
    12481 ccaggccgct ggtatgcaga gttgcggtgc tgatatcgcc cgagttggta atgttgccgg
    12541 tattggtgat gccatgcgtg gttgtcgcac cggaaaccga cagcgttccg ttgacggttg
    12601 tagcatcggt aatcgcattt cccaacgttg tattgccgtt taccgtcaga ttgttggtta
    12661 cctgagcgga attgagcgtt gccagaccgc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn ntcgcttatc gtggccagta agccgttttc tatactattt gcatcatccc
    12841 agaagctgag gtaggctttg tctgtgctgt tcaggcttaa cgttgcctca ttgcttgtca
    12901 tgctgtcaaa tgagtttaca acaatttccg tactgttggc catcaggccg ttggtcgcgc
    12961 cggtcgttcc atttaaataa ccgaccatta aattggttcc gtccgcggca agaccggaaa
    13021 tctcgccggt cgcataatta attgaaccga cggctgtgcc tatgccggct gatacgaggc
    13081 cgttgtaatt ggtgccatta tcatagccga tatatgcgcc ggacattgaa ggtcccatag
    13141 ggatggatat ttggcctaaa cccatacccg cggcacccgt tgcatcaggt gagtatgcgg
    13201 atataagaga attgttcacg gtcacgcctc tgaagctgcc cgcgttattg taaccaaggt
    13261 tgacttccga gttattggca tgaagtccat aagcctcgct tgaagaagta tccatataac
    13321 cgacaatcat gtcgttggta aaagtatcga cggtcattcc catgtttgtc gaaccgcttg
    13381 tcgagccgac catcaatccg gccggtccgg ctgccaggcc gctttcaacc gtaccgtcgt
    13441 tgaagctgat aaatgctttg tcggtgctgt ccagacttaa cgttgcttga tagcctgtgg
    13501 tgtaatctaa tgaatttgcc accgctctgc cgctgtcagc cataaaaccg tttctctttg
    13561 taacgccgtc gtcatagccc agttgcgcca gagaatcgtt tatatccatg ccgaaaagac
    13621 cgctggtgga ccgtaatgaa gaatcgccgt ttacattaaa caaaccggta accgttgtgg
    13681 aatcggtact accattgccc aacgttgtat tgcgtgtcac agagaagtct ctgacattgt
    13741 taatatcacg gacgttatta atgtcatgat tgcccatatt caggttgccg ccagcataga
    13801 tgacatcgcc tgtggtcagt gttccggcga cggtggtgtc gccataactg ccaaccgtaa
    13861 aaatatcgcc gtttgtgctg tcattaagca cggcgccgct gttgctcaat gtccaggtcg
    13921 tcgtgccggt gccaccggtc attgttgcac tggtcatgtc tgcacttaat ccgttccagt
    13981 tgttatttgc cagattagtg gtgccgatga gcatagagcc gtcgtcaaga tacagcaccc
    14041 cggtgccggc aacatcagga gtgtcaacgc cgttggtgtc cacgaaaaag ggaccatcta
    14101 taaaagatcc tgatctgtca acagtcaggc catagtgaag accagaattg tagtcgattg
    14161 atccgacact tatgccggtt gcatcggcta atagtccgtt ttcgattcta tttatagaat
    14221 cccagtagct gagatatgcg ttgctgccgc tgtccaggct taacgttgca gcattgccga
    14281 atgtataaga aaatgtggct ggatcatacg tgtaatctat tgaaaataaa ttggctcccg
    14341 gatcttgcgt gacttcccca gcaaaatagg cgttaatgta ataaccgctt acattggtgc
    14401 ttgtgcctgt atctccgggt gtagcgacaa acatatatcc gccacgcggc tcatctatgt
    14461 cgctagaaat acctcccgag tctacatacg ctccaactac gtctgccatg acctttcccg
    14521 ctaaaaaacc gccggacaaa aaaagtccga caaagagcaa ggctaaaatc attaaaaatc
    14581 tgtttttcat aataagcctc cttttaaaat taacttaaac attgagttat tgcctccttt
    14641 taaaaattct ttagcatctt tttttacgag ttgcctcgtt aatctatcga attaactcct
    14701 ttttaatgga tacgcatccg gcgcccgccg gcatcagaaa aacttttgtt gctcctccgt
    14761 cccggacata aatatgtctg gatagggctc ctgttctttt gaattttccg aattgttttt
    14821 ctgcgacttc cgaacatgtc tgcggccagt aaacgactgt ttcatacagc ccttcgcagt
    14881 aattttcctg ccccggcgca aaactggcgc tggcgtaggc cgtgttgtcc tgcaagggaa
    14941 tttccatgga tacggaagtc atgtgccgat caggccggct ggtcggatat atcatgatga
    15001 aaccgccgcc ctttgctccc gccgtaagaa aatttgtaac ctgattgatt cgccccgcac
    15061 aggtgcggac gccgtttttt aatgcggcct gagtgatggt gtttgccttc tgcgctgcgg
    15121 gttgaggagt ggcccggttt ttggaaaggg ccggagtgtt gctcgtttcg gcgctgaccg
    15181 ga
//
LOCUS       DAOSIM010000024        14982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_4121_length_14982_cov_7.173511, whole genome shotgun sequence.
ACCESSION   DAOSIM010000024 DAOSIM010000000
VERSION     DAOSIM010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 14982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14982
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4121_length_14982_cov_7.173511"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLR47_02165"
     CDS             <1..285
                     /locus_tag="PLR47_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11770.1"
                     /translation="TVKKATTTIDVLVTYADGRKAKTIKLCTQVDAGGAACTAPAVPS
                     YSASAPYTSTGAFTYTNPAGNGKVVSYWFVIVDEYDNEQTSDKKSFTTTN"
     gene            319..1434
                     /locus_tag="PLR47_02170"
     CDS             319..1434
                     /locus_tag="PLR47_02170"
                     /inference="COORDINATES: protein motif:HMM:NF025699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HOG11771.1"
                     /translation="MKNINIYKIMGNEKGMVMVIVLMLVAVLVILGTTGILTVTTDMK
                     ISSNYRENARAFYNAEAGAESVLAVLRTWTKANYPTSVGTPVTINLTVPSGFAFSSAV
                     QLYYVADNLYKFQIQGTGYNNASKVLEIYVKYTDPLPGGALAAVQANSAVENSGNMDI
                     DGRDYDISGNLITPVVGTKGVSSKSTVSTGGAGAIGGVDSAGNVSAPAKTPASGTVEQ
                     NATWTPPLTPDAALKIDEGTLKKIAKSGVNGSQYVTNPDALVLPLSGVTYVEADNWQA
                     VNFGNSSGILIVHNASRSAVLKNTNGGTFKGIMIIDDYVHEHNDMLGCVITLSTSPSS
                     GNVLGNGNGYIHYSTAAIGAALALVAGPSKTSWRDVQ"
     gene            complement(1446..2177)
                     /locus_tag="PLR47_02175"
     CDS             complement(1446..2177)
                     /locus_tag="PLR47_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11772.1"
                     /translation="MIYISVIFLAIWAYDFLYQPVVKRTVIFQSPVIAEVNGNNRQMA
                     SRREETVLLKNPVDHFQYMLAYHTSLVMKTNDIIDVWNNLPWGWISPFHIKAPIYFQT
                     TLSKNVAVTSLGKTIKSSVQTSHPITWTGMGNLFIWLTIWLIAPFCVFRVLTGRGKSL
                     DRLILIWIAVTYLPWFYVSLGVRRVVYSFYFINTVPALCLGIPHLIASVFTSAKTEKI
                     ILSLWLALTIGFFFYYFPVNVFQKW"
     gene            2248..2652
                     /locus_tag="PLR47_02180"
     CDS             2248..2652
                     /locus_tag="PLR47_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11773.1"
                     /translation="MTIAANITPHSLLKVERARARAAKKQRFSKYRYMPDIPKKRGAI
                     SSSRSDEWVKNIFSKARKAAAYKAWYSLRKCLDRPKKIQMVKRPKMTDGIRQANQELP
                     KIAMLNPAQALPTKGCSRLDALPSIRSRQAWT"
     gene            3083..6442
                     /locus_tag="PLR47_02185"
     CDS             3083..6442
                     /locus_tag="PLR47_02185"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HOG11774.1"
                     /translation="MNSEKSAISALETQKPLSTRGVFFVGLAYVALGAVGLTLAIPPG
                     YASPVFPAAGLALACVLLFGRRVLPAVWLASFLLNFSNAWLHGSLNPTTAAVAAVIAV
                     GATTQAWVGSLLIRRSMGEAWRGLRSEQDLFRFFLWGALAACLVSASISVSGLGLFGV
                     IESSEGFFTWWNWYLGDALGVMVFAPLVFCFLNRSDELWADRRRQIVVPMLLVLGLVW
                     LAFYGTARWEKQAMDRDIETDGISISNRIQDRLIAHREVLSSLGHFIKATPDFTFLQF
                     RKFTRLTLRDNPDISALSFNDQVPQEQREAYESKMSGLSPLGSYRIMERDQQQRLVPA
                     AARPEYVPVRYIVPLSINRQVVGYDIYSEPLRRNAIERARRTKAMAVTSPLQLVQDRE
                     KRVGLLGLLPVFAETEETGPQKERLLGFAATVIRVDEMVQIATRSKIPSNLVFRLTDA
                     DAPDGQNLLYRSEALDSASREWNQTPQWKTLLSVGDRSWELSIYVTKQYLREHRPWLA
                     WAVGVVGLIFSALLQMLLLGMTGQAMVIRRKNKEIQGLADSLENRVEERTAQLSEANQ
                     QLTREIAERNAAEEALTKSEEQFRTLVSNIPGIAYRCMNDPQWTMIFMSAEVDSVTGY
                     PASDFIGNAVRSYESIIHRDEIAVVEQAIAEALRQETPWEIAYRILHRDGGERWVYEK
                     GRGITGEDGTVRYLDGIIFDITERKRAEEELLRMNEEVERQAVQARQLALEAQQASRA
                     KSEFLASMSHEIRTPMNAIIGMAEMLQESPLTEEQTKYVRVFKAAGENLLNIINSILD
                     LSKVEAGQLSLESIPFSLRQVVEDVCVMMAVRVDKKQVRIFCRTASENCDSVIGDPTR
                     VQQILTNLIGNAIKFTEQGSVEVKVNTEKASFGGEKDDLCLVHISVKDTGIGIPPEKQ
                     GMIFERFTQADSSVTRKYGGTGLGLSISRRLAELMGGRLWVESTPGQGSTFHFTLLLR
                     IEDKTRQAAPPAKSEKAAVPEQRPLKILLVDDSDDNRLLVKTYLKGDAYQIDEAENGE
                     QAVALFQSGLYDLVLMDMQMPVMDGYTATRAIRRWEEEQGKNRTPVIALTAYALTEDA
                     AKSEEAGCDTHLTKPVKKAVLLEAIRRFAAP"
     gene            6613..8277
                     /gene="argS"
                     /locus_tag="PLR47_02190"
     CDS             6613..8277
                     /gene="argS"
                     /locus_tag="PLR47_02190"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOG11775.1"
                     /translation="MKKKLISLIARSVGACAGKGLLPDVDLSRIEVEVPANPEHGDYA
                     SNIAMVLASQARQNPRKIAQAIQENLDDPEGIVEKTQIAGPGFLNFMLKDDVWRSVLK
                     DILDQKDRYGCLPAGSGKKVQVEFVSANPTGPLHIGHARGAVVGDVLCNLLAATGQQV
                     SREYYINDAGNQMNNLGRSVLYRLRELAGEAVEFPENGYRGDYIREIAGDIFKKEGSR
                     FRDENEEETIQRFNEAAGAVILDGIRKDLRDFGVTFDCYFSERELYRENGVGRLLDEL
                     QKQGFIYSDGETLWFKTTDYGDDKDRVVIRKNGEPTYFAADIAYHANKFSRGFDLLID
                     IWGADHHGYIPRLEAAIQAIGRPREDLKIILVQLVNLLRAGEPVAMSTRSGEFVTMRE
                     VLDEVGRDAARYNFLMRRSDSHLDFDLEVAKKQSNENPVYYVQYAHARICSILRTAQE
                     RGMTLPDFPAIRPSLLAEAEERTLIKMMARYPEMLEGAAKTLEVHRITFYLNELAAVF
                     HGYYNKYKVVTQDAALSNARLVLVAAVREVLKNALGILGVGAPEKM"
     gene            8288..9049
                     /locus_tag="PLR47_02195"
     CDS             8288..9049
                     /locus_tag="PLR47_02195"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOG11776.1"
                     /translation="MGSGNTRKFEIRLGQTGLTVLIVGMVVLLCLSFLFGVGVGKNID
                     TYPEKIASLPQRFLALFWKPAKVAVEQKSVPVAQAGKANVDLAFHQALTSSKTVPIQP
                     LPDAEKTAEVEAFADREEPLPPPSSPSAQKEKAAPLKPEPVSPKKAAGEKPAAEIKSK
                     ESKTDSAAASYQVFVASLKDRQKADQIHRIVARAGYASKIVKVDLKGKGTWYRVIATG
                     FDTRAKAEAAADKLSKRAKTKCIVRPTAAGKAANP"
     gene            9119..10447
                     /gene="ffh"
                     /locus_tag="PLR47_02200"
     CDS             9119..10447
                     /gene="ffh"
                     /locus_tag="PLR47_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOG11777.1"
                     /translation="MFESLSEKLEGIFKKLKGRGVLNEESVNAALREIRMALLEADVN
                     FKVVKDFIEDVRGRAVGKEVLESITPGQQVVKIVHDRLVELMGSTSAAIKFGPRIPAP
                     IMLVGLQGCGKTTTAAKLARLISADKRVYLVSADVYRPAAMEQLAVLGRQVKAGVFEA
                     GSCKDPVKICVDAVEQARREGYEVILIDTAGRLHIDEELMDELRRIREKVNPAEILYV
                     ADAMTGQDAVNVGAKFNELIGIDGVIMTKMDGDARGGAALSLRSVVGKPIKFVGVGEK
                     IDALEVFHPERMAGRILGMGDILSLVEKAQATIDEKTAKQMQESLRKSDFTLEDFRKQ
                     LGQIKKMGPLKDMIGMIPGMGKIKALKDMEPDDGELVKIAAMIDSMTKKERNNYLIID
                     GRRRKRIALGSGTTVQDVNRMLKNYIELRKMMKKINSKGGMKALMRNFPF"
     gene            10512..10766
                     /gene="rpsP"
                     /locus_tag="PLR47_02205"
     CDS             10512..10766
                     /gene="rpsP"
                     /locus_tag="PLR47_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOG11778.1"
                     /translation="MAVKIRLTRMGAKGKPFYRIVAADKEYPRDGRFLEILGNYDPKK
                     NPAEIKLKEDRVRDWLSKGAKPTLTVSHLLKTKGIVAAVK"
     gene            10894..11124
                     /locus_tag="PLR47_02210"
     CDS             10894..11124
                     /locus_tag="PLR47_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOG11779.1"
                     /translation="MKDLIKYISQALVDNPDQVEVTEVIGEQTSVIELRVAKEDLGKV
                     IGKQGRTAKAIRTILSATSAKVHKRAVLEIIE"
     gene            11126..11632
                     /gene="rimM"
                     /locus_tag="PLR47_02215"
     CDS             11126..11632
                     /gene="rimM"
                     /locus_tag="PLR47_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOG11780.1"
                     /translation="MDLFVFGEIVKTRGLHGCLKALSFLESSELLDGLTDVYLETPSG
                     RKSRHEIKKINSSGKFLFLEIKDVSGVDEARRLVGSRLLMPRDRLAALAADEYYWHDI
                     VGLDVYTPRGRHLGRIESVFPTGGNDVYVCRGDAGEILIPATAEVVLQVDLAGKKMII
                     QPLEGLLP"
     gene            11629..12366
                     /gene="trmD"
                     /locus_tag="PLR47_02220"
     CDS             11629..12366
                     /gene="trmD"
                     /locus_tag="PLR47_02220"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOG11781.1"
                     /translation="MIRFDVLSIFPEMLQSPLEFSLLKKARDKGLVEVGLHNIRDWAE
                     DKHRMTDDAPYGGGCGMVMKVEPVARALAAVKRPVGPSLTVLMTPQGEIFQQKIAAEL
                     ASQEQIILICGRYEGFDERIREHLADREISLGDYILTGGELSALVVIDAVSRLIPGVL
                     GNEDSASRESFSQGLLEYPQYTRPASFSGWSVPEVLVSGNHAQIERWQRIEALRRTWQ
                     RRPELLEKAGLGGEDEELLEKIKLGRI"
     gene            12457..12804
                     /gene="rplS"
                     /locus_tag="PLR47_02225"
     CDS             12457..12804
                     /gene="rplS"
                     /locus_tag="PLR47_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOG11782.1"
                     /translation="MNVLEMIEKEQMRGDIPAFKSGDTVKVYVRITEGQKQRIQAFEG
                     VVIRKRRGNSRANFTVRKVSYGIGVERTFPIHSPVIDRIEVVSRGLVRRSRLYYLRSL
                     RGKKARIKEDTGH"
     gene            12824..13447
                     /locus_tag="PLR47_02230"
     CDS             12824..13447
                     /locus_tag="PLR47_02230"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOG11783.1"
                     /translation="MNTFERLAQVEGYKTIAGVDEAGRGPLAGPVVAAAVIFPPEYSN
                     PAINDSKKLTAAKREDLYRVITREAMAVGLGVVEADVIDRINILQASLQAMREAVTEL
                     SVRPDYLLIDGLNSIDFDAPQKAIVKGDSLSVSIAAASIIAKVSRDRIMEMYHRQFPR
                     YNFLKNKGYGTVGHRRVLEEIGMCKIHRRSFHLKKQNLPQTALDWSE"
     gene            13444..13830
                     /locus_tag="PLR47_02235"
     CDS             13444..13830
                     /locus_tag="PLR47_02235"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOG11784.1"
                     /translation="MKDSQDRRKIITGRAGEAMAADFLKRRGYRILEMNYRCSIGEID
                     MVAREKDVLVFVEVKTRKSGAMGYPEQAVGLVKQKKMSQLALWYLRDKNIGDAAVRFD
                     VVAILLLPSGNDIRLIRGAFDFIALE"
     gene            complement(13878..>14501)
                     /locus_tag="PLR47_02240"
     CDS             complement(13878..>14501)
                     /locus_tag="PLR47_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HOG11785.1"
                     /translation="HAVGTDSRIGAKFLKAGIGFGGSCFKKDILNLVYLCRFYGLPEV
                     ASYWESVVRINEYQQDRFIANILRSMFNTLAGKKICVLGFAFKADTGDTRESPAIAIT
                     RKLLAERAEVIITDPRALANAAIDLAHEKGNIRYVEDPYEAARGCHALAVMTDWEEYR
                     KLDYRKIYKSMVKPAFLFDGRNIVDHRKCFEIGFNVFPIGKPALTRF"
     assembly_gap    14502..14517
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14518..>14982)
                     /locus_tag="PLR47_02245"
     CDS             complement(<14518..>14982)
                     /locus_tag="PLR47_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOG11786.1"
                     /translation="FVCVNTPTKTFGAGAGMAADLQYWEKTARQILENAKSPKIVIEK
                     STLPIKTAQAMERILSSGKKNITFDVLSNPEFMAEGTAVSDLENPDRVLIGSRETKSG
                     LAARAALVEIYTRWLPREKIITSNIWSAELSKLVANAFLAQRVSSINSISALC"
BASE COUNT         3680 a         3826 c         4368 g         3092 t
ORIGIN      
        1 acggttaaaa aagcgaccac cacgattgat gttttggtta cctatgctga cgggcgcaag
       61 gcgaaaacga ttaagttgtg cacccaggtt gacgcaggag gcgctgcctg cacagctccg
      121 gccgttccgt cttattccgc gtcagcgcct tatacctcga caggtgcctt tacttacacc
      181 aatcccgccg gaaatggtaa agttgtgtcc tactggtttg tcatcgttga cgaatacgac
      241 aatgaacaga ccagtgacaa gaagtcattc acaacaacaa attaatctcc tgagagcagg
      301 cagggttatg ggagttgtat gaaaaacatc aacatataca agatcatggg caacgaaaag
      361 ggcatggtga tggttattgt gctcatgcta gtggccgtgc tggtgattct gggaacaacc
      421 ggtattctga ccgtaacaac ggatatgaag atttccagca actataggga aaacgccagg
      481 gctttttata acgccgaagc cggtgcagag tcagtgctgg ccgtgctgcg cacgtggact
      541 aaagcaaatt atcccacgtc ggttggcact cctgtaacaa taaatttgac ggtgccttca
      601 ggctttgctt tcagctctgc ggtgcagctt tattatgtgg ctgataatct ttacaaattt
      661 caaattcaag gaacaggata caacaatgct tcaaaagttc tggaaattta cgttaaatat
      721 acagatcccc ttcccggagg cgcgctggca gccgtacagg ccaacagtgc cgtggaaaat
      781 agtggcaata tggatataga cggaagggat tatgatatta gcggcaatct cattacacca
      841 gtagtcggta caaaaggagt cagcagcaaa tccaccgtaa gtactggcgg cgccggtgca
      901 atcggaggtg tagatagcgc aggaaatgtg tctgcaccag ccaagacccc tgcttcagga
      961 accgttgaac aaaacgccac ttggacaccg ccgcttacgc ctgatgcggc tctaaaaatt
     1021 gatgagggaa ctctgaaaaa aatcgctaaa agcggcgtga acggatcaca gtatgtaaca
     1081 aatcccgatg cgcttgttct gcccttatca ggtgttacat acgttgaagc cgataattgg
     1141 caggctgtaa attttgggaa cagcagcggc atattgattg ttcacaacgc atcaagaagt
     1201 gcagtcttaa agaatacaaa cggcgggact tttaagggca tcatgatcat agatgactat
     1261 gttcatgaac ataacgatat gttgggctgc gtcatcactt taagcacttc gccttcatca
     1321 gggaatgtgc tcggcaatgg caatggatat atacattaca gcacagccgc aatcggcgcc
     1381 gctttagcgc ttgtggcggg accgagtaaa acctcctggc gtgatgttca gtgataaaac
     1441 ccaattcacc atttttgaaa aacattgacg gggaaatagt aaaagaaaaa gccgatggtc
     1501 aaagccagcc acagggagag gataattttt tccgtcttgg cggaagtaaa cacagaggca
     1561 atcaggtgag gaatgcccaa acacaaggcc gggacggtgt tgatgaaata aaagctgtaa
     1621 acaacgcgac gaacgccaag cgacacgtaa aaccacggca aataagtaac cgcgatccag
     1681 ataagaatca gacgatccag agatttgccg cgcccggtta aaactctaaa gacgcaaaaa
     1741 ggcgcaatca gccaaatcgt cagccagatg aagaggttgc ccatacccgt ccatgtaata
     1801 ggatgggacg tttgaacgga tgatttgatc gtttttccca aagatgtcac cgccacgttt
     1861 tttgaaaggg tggtttgaaa atagatgggc gccttgatat gaaagggact gatccatccc
     1921 cagggcagat tattccaaac gtcaataatg tcatttgtct tcatgaccag cgatgtatgg
     1981 taggccagca tgtactggaa atggtcaacg ggatttttaa gcagcacggt ttcttcacgg
     2041 cggctggcca tttgcctatt atttccgttt acctcggcaa taacaggtga ttgaaaaata
     2101 accgtccttt ttacaaccgg ctggtaaaga aaatcgtagg cccaaatggc cagaaaaatg
     2161 acgctgatgt aaatcaatag aaattttcct gattgtcgca ggttttcccc gtccatcagt
     2221 cgcctgtttt gaaaaagatg atagataatg acgattgccg caaacattac tccccattct
     2281 ttgcttaaag tggagagggc aagagccaga gccgcaaaaa agcagcgctt ttctaaatac
     2341 agatatatgc ctgatattcc aaagaaaaga ggcgcaatat ccagcagcag aagcgatgaa
     2401 tgggtgaaaa atatattttc aaaagccaga aaggcagccg cgtataaagc ctggtattca
     2461 ttaagaaaat gtctggacag gccaaagaaa atccagatgg tgaaaaggcc gaaaatgacg
     2521 gatggaatac gccaagcaaa ccaggaattg ccgaaaattg caatgcttaa tcctgcccag
     2581 gccttgccga caaagggatg ttccagattg gacgctttgc catcgataag atcaaggcag
     2641 gcctggacat aatttgcttc atcaaaaact ttacctccat cactgggaaa aggaataaca
     2701 aagagatgca gagccagcga aatcaagagc agatttacaa gccagggaga aatcgtcggg
     2761 tatttaaaag ttgaggagtt gattgttttt ttcctttaca atttttctat aaaagcgaac
     2821 ttaattccgg tccaatgcgg acattaagaa aacttccgat gtccgtgccg ataatagaaa
     2881 tgactaccac gaaagtcaaa caaatctttc agaaataatg gcagcagccg caagttcatt
     2941 ctgcaatgac aagcggcaag aaagttccct gcgtatttga ctgtgtaaga gtttgagatt
     3001 attcgattga cacacacggt cattttccat atgatttttt tcagaaaaaa cgtgaaatca
     3061 tccttgcgaa ggaacagcat ttgtgaattc ggaaaaaagc gccatctccg cacttgagac
     3121 tcaaaagcct ctttccacga gaggagtgtt ctttgtcgga ctggcttatg tcgcgctggg
     3181 cgccgtcggc ctcacactgg ccattccgcc cggctatgca agccccgttt ttcccgccgc
     3241 gggcctcgcg ctggcctgtg tgcttctgtt cggccggaga gtgcttcccg ccgtctggct
     3301 ggcttctttt ttgctgaatt tctccaacgc ctggctccac ggctcgctca atccgacgac
     3361 ggcggccgtg gcggcggtga tcgccgtcgg cgcgaccacg caggcctggg ttgggtccct
     3421 gctgatccgg cgctccatgg gtgaagcctg gcgcggcctg agaagcgaac aggatctctt
     3481 tcgttttttt ctttgggggg cgctggcggc ctgcctggtt tcggcctcga tcagcgtgag
     3541 cgggctgggt ttgttcggcg tcattgaatc ttcggaaggg ttcttcacct ggtggaactg
     3601 gtacctgggc gatgccctgg gcgtgatggt ttttgcgccg ctggtctttt gttttctcaa
     3661 caggtccgat gaactctggg cggacaggcg ccgtcagatc gtcgtgccca tgctgctggt
     3721 tctggggctg gtgtggctgg ctttttacgg aacggcccgc tgggaaaaac aggccatgga
     3781 cagagatatc gagacggacg gcatttccat ttccaaccgt attcaggacc ggctgatcgc
     3841 gcaccgggaa gtgctttcct cgctgggcca tttcatcaaa gcgacgccgg atttcacctt
     3901 tcttcagttt agaaagttta cccggctcac gcttcgggac aaccccgaca tttccgcact
     3961 cagcttcaac gatcaggttc cccaggagca gcgggaggcc tacgaaagca aaatgagcgg
     4021 cctgtcgccg ctgggttctt accggatcat ggagcgggac caacagcagc ggctggttcc
     4081 ggccgccgca aggccggaat acgtgccggt gcgctacatt gtccctttga gtataaaccg
     4141 gcaggtggtg ggatatgata tttactccga gccccttcgc cggaatgcga tcgaacgcgc
     4201 ccggcgaaca aaagccatgg ctgtgacgtc tccgctgcag ctggtgcagg atcgtgaaaa
     4261 gcgcgtcggt cttctggggc tgttgccggt ttttgcggaa acggaagaaa ccggaccgca
     4321 aaaggaacgc ctgctaggct ttgccgcgac cgtgatccgg gtcgatgaaa tggtccagat
     4381 cgcgaccagg agcaaaatcc cttcgaacct ggtctttcgg ttgaccgacg cggatgcgcc
     4441 cgatggacaa aatctgctgt accgttccga agctttggat tccgcctcca gggaatggaa
     4501 ccaaacgccg cagtggaaga ccctgctttc tgttggcgac cgcagttggg agctgtcgat
     4561 ttacgtcacg aaacagtacc tgcgagagca ccggccctgg ctggcctggg cggtgggcgt
     4621 tgtggggctg attttctcgg cgcttcttca gatgctgctt ctgggaatga ccgggcaggc
     4681 catggtgatt cgtcgaaaga acaaagagat tcaaggactg gccgactccc tggaaaaccg
     4741 ggtggaggaa cgaacggcgc agctctcaga ggccaatcaa caattgacgc gggagattgc
     4801 cgaacgcaac gcggccgaag aggcgctgac aaaaagcgaa gagcagttca gaacccttgt
     4861 gtcgaatatc cccggcatcg cctaccggtg catgaacgac ccgcaatgga cgatgatctt
     4921 tatgagtgcg gaggtcgatt cggttacagg ttatccggct tccgatttta tcggcaatgc
     4981 cgttcgctcc tatgagagca ttattcaccg ggatgaaatc gccgtagttg aacaagccat
     5041 tgcagaggcg ctgcggcagg agaccccctg ggaaattgca taccggattt tgcaccgcga
     5101 cggcggcgag cgctgggtgt atgaaaaagg ccgcggcatc accggcgagg atgggactgt
     5161 ccgctatctg gacgggatta ttttcgacat tacggagcga aaaagggcgg aggaagagct
     5221 gctccggatg aacgaggagg tagaacggca ggccgttcag gcccgtcagc tggccttgga
     5281 ggcgcagcag gccagccggg cgaaatcgga gtttctggcc agcatgagcc atgaaatccg
     5341 gacgccaatg aatgccatca tcggcatggc ggaaatgctg caggagagtc ctctcacgga
     5401 agagcagacc aagtatgtcc gcgtcttcaa ggcggcgggg gaaaacctcc tcaatatcat
     5461 caattcgatt ctggatctgt ccaaagtgga agccggacaa ctgtcgctgg aatcgattcc
     5521 gttcagcctg cggcaggtag tcgaggatgt ctgcgtcatg atggccgtgc gggtcgataa
     5581 aaagcaggtg cggatttttt gccggacggc ttcggaaaac tgcgattccg tcattggtga
     5641 tccgacccgt gtgcagcaga tcctgacgaa tttgataggc aacgccatca aattcacgga
     5701 gcagggcagc gtggaggtaa aagtaaatac ggaaaaagcg tccttcggcg gcgaaaaaga
     5761 cgatctttgc cttgtgcaca tttccgtaaa ggacacgggc atcggcattc cgccggaaaa
     5821 gcagggcatg atttttgagc gctttaccca ggccgactcc tccgtaacga gaaagtacgg
     5881 aggaaccggt ctgggcctgt ccatttcccg ccgtctggcg gagctgatgg gagggcgcct
     5941 ctgggtggaa agcacgcctg gtcagggaag cacgtttcat tttacgcttt tgctgcgcat
     6001 tgaagacaaa acacggcagg cggcgccgcc tgcaaaaagc gaaaaggcgg ctgttcccga
     6061 acagcgcccg ttgaaaattc tgctggtgga tgattcggac gacaaccgtc ttctggtcaa
     6121 gacgtatctc aagggcgatg cctatcaaat cgatgaggcg gaaaacggcg agcaagcggt
     6181 agcgcttttc cagtccggtc tttatgatct ggtgctcatg gacatgcaga tgccggtgat
     6241 ggacggttat acggccacgc gcgccattcg ccggtgggaa gaggagcagg gcaaaaatcg
     6301 gacgccggtg atcgcgctga ccgcctacgc cctcacggaa gacgcggcca aaagcgaaga
     6361 ggccggctgc gacacgcatc tgaccaaacc cgtcaaaaaa gcggttctcc tcgaggccat
     6421 ccgccggttt gccgctccct gagccccatc cgccgttgac agttgagctt ggggagaaaa
     6481 ggggatttac acggcgttgc acccttcaaa cagcaattat caacagtcag aatctaatag
     6541 ttcatcttgc agtgaggccc ggcaagtgat aaaagcctcc agccattata ggaaaggttt
     6601 gcatagatat tcatgaaaaa gaaactgatt tccctgatcg cgcgttccgt cggggcctgc
     6661 gccggcaagg gactgctgcc ggacgtggat ctttcgcgca tcgaagtcga agtgccggcc
     6721 aatccggagc acggggacta cgcctccaat atcgcgatgg ttctggcctc ccaggccaga
     6781 caaaacccgc gcaaaatcgc ccaggcgatt caggaaaacc tcgatgatcc ggaaggcatc
     6841 gttgaaaaaa cgcaaatcgc cggccccggc tttttgaatt tcatgctcaa agacgatgta
     6901 tggcgaagcg ttttgaaaga tattctggat cagaaggacc ggtacggttg ccttccggca
     6961 ggctccggga aaaaggtcca ggtggaattc gtcagcgcca atccgacggg gccgctgcac
     7021 atcgggcacg cgcggggagc ggtggtgggc gatgtgctct gcaatctgct ggcggcgacc
     7081 ggccaacagg tttcccggga atattacatc aacgacgcgg ggaaccagat gaacaacctg
     7141 ggccggtccg tgctgtatcg tctgcgggag cttgcgggag aagccgtcga atttccggaa
     7201 aacggctacc gcggagacta catcagggaa atcgccggcg atattttcaa aaaggaagga
     7261 agccgtttcc gggatgaaaa cgaggaggaa acgattcagc gcttcaacga agcggcgggc
     7321 gcggtgattc tcgacggaat tcggaaggat ttgcgggatt tcggcgtgac ctttgactgc
     7381 tatttcagcg agagggagct gtaccgggaa aacggcgtcg gacgcctgct ggacgaactg
     7441 caaaagcagg gcttcattta ttccgacggg gagacgctgt ggtttaaaac gaccgattac
     7501 ggcgacgaca aggaccgggt ggtcatccgg aagaacggag agccgaccta cttcgcggcc
     7561 gacatcgctt atcacgccaa caagttttcc cgcgggttcg atctgctcat cgacatctgg
     7621 ggcgccgacc accacggcta cattccccgc cttgaggccg cgattcaggc catcggacgg
     7681 cccagggaag atctgaaaat cattctcgtg cagctggtca acctgctgcg cgcgggcgag
     7741 ccggtggcca tgtccacgcg ctccggcgaa ttcgtcacga tgcgcgaagt gctggacgaa
     7801 gtgggcagag atgcggcccg ctataatttc ctgatgcgcc gctccgacag ccatctggat
     7861 ttcgacctgg aagtcgccaa gaaacagtcc aacgaaaacc cggtttacta tgtccagtac
     7921 gcccacgccc gaatctgcag cattctccgg acggcccagg agcggggaat gaccctgcct
     7981 gattttccgg cgatccgtcc atccctgctt gccgaagcgg aggaaagaac gctgatcaaa
     8041 atgatggcgc gctatcccga aatgctggaa ggggcggcaa agacgctgga ggtccaccgc
     8101 atcacctttt atctgaatga gctggctgcc gtatttcacg gctactacaa caagtacaag
     8161 gtagtcaccc aagacgcggc gctatcgaac gccaggctgg tgctggtcgc ggcggtgcgc
     8221 gaggtgctga aaaacgcgct tggaattctg ggcgtgggcg cgccggaaaa aatgtaggag
     8281 ggaggccatg ggctcgggga ataccaggaa atttgaaatc aggctggggc agacgggatt
     8341 gacggtcctg atcgtcggca tggtcgtcct gctgtgcctt tcctttcttt tcggcgtcgg
     8401 cgtaggaaaa aacatcgaca cctatcccga aaagattgcc tccctgccgc agcggtttct
     8461 ggcgctcttc tggaagccgg ccaaggtcgc cgtagagcag aaatcggttc ccgttgcgca
     8521 ggcgggcaag gccaatgtgg acctggcgtt tcatcaggcg ctgaccagtt ccaaaaccgt
     8581 cccgattcaa cccctgccgg atgccgaaaa gacggccgag gttgaagcct tcgcggacag
     8641 ggaagaacct ttgccgccgc cttcatcgcc atcggctcaa aaggaaaagg ccgccccgtt
     8701 gaaacctgaa ccagtcagcc cgaagaaggc cgccggcgaa aagcccgccg cggaaatcaa
     8761 atcaaaagaa agcaagaccg attccgccgc cgcatcctat caggtgttcg tggcgtcgct
     8821 caaagacagg caaaaggccg accagattca caggatcgtc gcccgggcgg gatacgcgtc
     8881 gaaaatcgta aaggtggatc tgaaagggaa gggcacgtgg tatcgcgtga tcgccacggg
     8941 attcgacacc cgcgccaaag ccgaggcagc cgccgacaag ctttccaaga gggccaaaac
     9001 gaaatgcatc gtgcgcccaa ccgcggccgg aaaggcggcg aatccctgag gaaacaaagc
     9061 cggccccgca cgggcgaggc gcgatgaggg aaacggaaat attgtgaagg tttgaaatat
     9121 gtttgaaagt ctttcggaaa aactggaagg tatttttaaa aagctcaagg gaagaggcgt
     9181 tctcaacgag gaaagcgtca atgccgcgct tcgggaaatc cggatggcgc ttttggaagc
     9241 ggacgtaaac ttcaaggtcg tcaaggattt tatcgaagac gttcgggggc gcgccgtcgg
     9301 caaagaagtg ctggaaagca tcacgcccgg acagcaggtg gtcaagatcg tgcacgaccg
     9361 cctggtggag ctgatgggct ccaccagcgc cgccatcaag ttcggtccga ggatccccgc
     9421 gccgattatg ctggtcggcc ttcagggctg cggaaaaacg accacggccg ctaaactggc
     9481 gcggctcatt tccgcggata aaagagttta cctggtgtcc gccgacgttt accggcccgc
     9541 cgcgatggag cagcttgccg ttttgggccg gcaggtcaag gccggcgttt ttgaagccgg
     9601 ctcttgcaaa gatcccgtaa aaatctgcgt ggacgcggtc gaacaggccc gaagggaagg
     9661 ctatgaagtc atcctgatcg acaccgccgg acggctgcac atcgacgagg agctgatgga
     9721 cgaactccgc cggatcaggg aaaaggtcaa cccggccgaa attctgtatg tggccgacgc
     9781 gatgaccggc caggacgcgg tcaacgtcgg ggccaagttc aacgagctca tcggcattga
     9841 cggcgtgatc atgaccaaga tggacggcga cgcgcgcggc ggcgctgccc tgtcgctgcg
     9901 atccgttgtg ggcaagccga tcaagtttgt cggcgtcggc gaaaagatcg acgcgctgga
     9961 ggtttttcat ccggagcgga tggccggccg gattctcggc atgggggaca ttctgtcgct
    10021 ggtggaaaaa gcgcaggcca ccatcgatga aaaaaccgcg aagcagatgc aggaaagcct
    10081 gcgcaagagc gattttacgc tggaggattt ccgcaaacaa ctggggcaga tcaaaaaaat
    10141 ggggcccctg aaggacatga tcggaatgat cccgggcatg ggcaaaatca aggcgctgaa
    10201 agacatggag ccggacgacg gggagctggt gaaaatcgcg gcgatgattg attccatgac
    10261 gaagaaagaa cgcaacaatt acctgatcat agacggccgc aggcgcaagc ggatcgccct
    10321 gggaagcgga acgacggttc aggacgtaaa ccgcatgctg aaaaactaca tagagcttcg
    10381 gaaaatgatg aaaaaaataa attccaaagg cggcatgaaa gcgttgatga ggaatttccc
    10441 cttttaaaat caaaatattt gtgataaaaa atctgacact tttttttgaa ggaggtttgg
    10501 agtaaaacta catggccgtt aaaatcagat tgacccgcat gggtgcgaaa ggcaaaccgt
    10561 tttaccgtat cgtcgccgcg gataaagaat atccccgcga tggaagattc cttgaaatat
    10621 taggaaatta tgatcccaag aaaaacccgg cggaaatcaa actcaaagag gaccgcgttc
    10681 gcgactggct gtccaaaggc gcaaagccga cgctgaccgt ttctcattta ttgaaaacaa
    10741 aaggcatcgt tgccgccgtc aagtagacgg gccgcggccg atcacgcttg caaaatacag
    10801 gattagaacc tcacaccatg gaatgaaaca agctgtgtga atgttggaat gatttttcag
    10861 acgcattaac caaaaccagt ggaggggctg acgatgaaag acctgattaa gtacatttct
    10921 caagctttgg ttgataatcc tgatcaagtg gaagtcacgg aagtcatcgg agagcagacc
    10981 tccgtcatcg aactgcgcgt ggccaaagag gacctgggca aagtcatcgg caagcaggga
    11041 aggacggcca aagccatccg caccattttg agcgccactt ccgccaaagt ccacaagcgg
    11101 gcggtgctgg aaatcatcga gtaaaatgga tttgtttgtt ttcggggaga tcgtcaaaac
    11161 ccgcggtctc catggctgtt tgaaagccct gtcttttctg gagtcatcgg agcttctcga
    11221 cggactgacg gatgtgtacc tggaaacccc gtccggccgg aaaagccgtc atgaaataaa
    11281 aaaaataaat tcctccggta aattcctgtt tctggaaatc aaggatgtgt ccggcgtgga
    11341 cgaggcccga aggcttgtcg gatcgaggct gctcatgccg cgcgacaggc ttgcggctct
    11401 tgcggccgac gagtattact ggcacgacat cgtcggactt gacgtttaca cgccccgggg
    11461 ccgccatctg ggaaggatcg agtccgtctt tcccaccggc ggcaatgatg tctatgtctg
    11521 ccggggggac gcgggtgaaa tcctgattcc ggcgacggcc gaagtggtcc tgcaggtgga
    11581 ccttgccgga aaaaaaatga tcatccaacc gctggaaggc cttctgccgt gatccgattt
    11641 gacgttttgt ccatttttcc ggaaatgctg caatcgcccc tggagttcag ccttttgaaa
    11701 aaggcgcggg acaagggact tgtcgaagtc ggcctgcaca acattcggga ctgggcggaa
    11761 gacaaacacc gcatgaccga cgacgccccc tacggcggcg gctgcggaat ggtgatgaaa
    11821 gtcgagccgg tggcgcgggc gctggcggcg gtgaagcgcc cggtggggcc gtcgcttacg
    11881 gttctgatga cgccccaggg ggaaatcttt cagcagaaga tcgcggctga gctggcgagc
    11941 caggaacaga tcatcctgat ttgcggacgg tatgaaggat ttgacgaacg gattcgggag
    12001 catctggcgg accgggagat ttccctgggc gactacattc tgacgggagg ggaactttcc
    12061 gcgctcgtgg ttattgatgc cgtctccagg ctgattcccg gcgtgctggg aaacgaagac
    12121 tccgcgtcga gggaatcttt ttcgcagggg cttttggaat atccgcagta cacgcgcccc
    12181 gccagttttt cagggtggag cgttccggaa gtgctggttt ccggaaacca tgcccagatc
    12241 gagcgctggc aacgcatcga ggcgcttcgc cgcacctggc aaagaaggcc cgaactgctc
    12301 gaaaaagccg ggctcggcgg ggaagatgag gaacttttgg aaaaaattaa gctgggccgg
    12361 atataaagtc ttgattttca gacgggtttg gggtaatgaa accagccttt agggcacagc
    12421 gattcgtatt tgaaaagcag caggaggagt gaggacatga atgtattgga aatgattgaa
    12481 aaagaacaga tgagaggcga tattcccgcc ttcaaatcgg gcgacaccgt caaggtatat
    12541 gtgcggatca ctgaaggaca gaagcagaga atccaggctt ttgaaggcgt ggtcatccgc
    12601 aagcgccgcg gcaacagccg ggcaaatttc acggttcgga aggtgtccta cggcatcggc
    12661 gtagaaagaa cctttcccat tcattcgccg gtcattgatc gcatcgaagt ggtgtcccgc
    12721 ggtttggttc gccgttcgcg cctgtattac ctgagaagcc ttcgagggaa aaaggcaagg
    12781 atcaaggaag acaccggcca ctgaggccgt tcagggtttt tcgatgaata cattcgaaag
    12841 acttgcgcaa gtggaaggct acaaaaccat cgcgggtgtt gacgaagcgg gccgcggccc
    12901 gctggccggc cccgtggtgg ccgcagccgt aatttttccg cccgaatatt caaatcccgc
    12961 catcaacgat tccaagaaac tgaccgccgc caaacgcgaa gatctctacc gggtcatcac
    13021 ccgggaggcg atggccgtag gcctcggcgt ggtggaagcg gacgtcatcg accggatcaa
    13081 cattcttcag gcgtcgcttc aggcgatgcg cgaagcggtg acggagcttt ccgtgaggcc
    13141 ggattacctc ctcatcgacg ggctcaactc catcgacttc gacgcgccgc agaaggcgat
    13201 cgtcaaaggg gattcactca gcgtttccat cgccgccgcg tccatcattg ccaaggtttc
    13261 acgcgaccgg atcatggaga tgtatcaccg ccagtttccc cgctacaact ttctgaaaaa
    13321 caaaggatac ggcacggtcg ggcatcgcag ggtccttgag gaaatcggca tgtgcaagat
    13381 tcatcgccgg tcctttcatc tcaaaaaaca gaatctcccg caaacggcgc tggactggtc
    13441 agaatgaagg attcacaaga caggcgaaaa atcatcaccg gcagggccgg ggaagcgatg
    13501 gccgccgatt ttctgaagcg gcggggctac cggatacttg agatgaacta ccgctgttcc
    13561 atcggggaga tcgacatggt cgcccgcgag aaggacgtgc tggtgtttgt ggaggtcaag
    13621 acgcgaaaat ccggcgcgat gggctatccc gagcaggcgg tgggtttggt caaacaaaag
    13681 aaaatgtctc agctcgcgct gtggtatctg cgggataaaa atatcgggga cgcagcggtc
    13741 cgctttgacg tggtggccat tttgctgctt ccctccggaa acgacattcg actgatccgc
    13801 ggcgctttcg acttcattgc cctggaatag cccgcgttgt gcctgcggct ttgccggcag
    13861 gcgccggacg cgagaagcta aaagcgggtg agggccggct ttccgatggg aaacacgttg
    13921 aagccgatct caaagcattt tcggtgatcg acgatgttgc ggccgtcgaa caggaaggcg
    13981 ggtttgacca tcgatttgta gatcttccgg taatccagct tccggtattc ctcccagtcc
    14041 gtcatgacgg ccagcgcgtg gcagccccgc gcggcctcgt aggggtcttc gacataacgg
    14101 atatttccct tttcatgcgc cagatcgatc gcggcattgg ccagcgcccg gggatcggta
    14161 atgatcactt cagcccgctc cgccaggagc tttcgtgtga tggcgatggc cgggctctcc
    14221 cgcgtgtcgc cggtgtcggc tttgaaggca aatcccagaa cgcagatttt ctttccggcc
    14281 agcgtgttga acatggaccg cagaatgttg gcgataaacc ggtcctgctg atactcgttg
    14341 atcctgacga cgctttccca gtacgaggcc acttccggca gaccgtaaaa acggcacagg
    14401 tacacgaggt tcagaatatc tttcttgaag caggagccgc cgaagccgat gcccgccttc
    14461 agaaatttgg cgccgatgcg gctgtccgtg cccacggcgt gnnnnnnnnn nnnnnnnaca
    14521 aagcgccgat atggaattga tggaggatac ccgctgcgcc agaaaggcgt tcgccaccag
    14581 cttggaaagt tcggcgctcc agatgttcga ggtaatgatc ttttctcgcg gcagccaccg
    14641 ggtgtaaatc tccacaagcg ccgcacgggc ggccagtccg cttttggttt cgcgcgagcc
    14701 gatcagaacg cggtccgggt tttccaggtc gctgacggcg gttccttccg ccatgaattc
    14761 cgggttggaa aggacgtcaa acgtgatgtt tttcttcccc gacgacagaa tccgctccat
    14821 cgcctgcgcc gtcttgatcg gcagcgtgct tttttcgatc acgattttcg gggatttggc
    14881 gttttcgaga atctgccggg cggttttttc ccagtactga aggtcggcgg ccatgccggc
    14941 gcccgcgccg aaggttttgg tcggcgtgtt gacgcagacg aa
//
LOCUS       DAOSIM010000025        14523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_4260_length_14523_cov_7.597733, whole genome shotgun sequence.
ACCESSION   DAOSIM010000025 DAOSIM010000000
VERSION     DAOSIM010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 14523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14523
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4260_length_14523_cov_7.597733"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(393..2600)
                     /locus_tag="PLR47_02250"
     CDS             complement(393..2600)
                     /locus_tag="PLR47_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11787.1"
                     /translation="MISGSIFRKSKIICFLLGLMLCSPPGAAFAVDVQAIFPGWSVSG
                     QNSMHLDYYNSHGPDGAYLQEGWRPYNDLNIRFQNIQNPYNTIKGYASGAISGSPYRS
                     PQYGVILERFNINQENGEAPVPYRMDLGDFHGFQTPRVLQRSLKGAQLELQPGQFLGA
                     QNSLLLQGGGASRDFFDNRLADNLFGGASWMMNWSRTRASLNFAYNDQAPQSGTPDLR
                     QYVTGLAVNHNFNFLAQQLSLDAELNYLRGDNAQNGESFKDEGLGAFAQFQGRGKIPL
                     TYSLRYEEYDKAYIPRGGVVSAGRRTIDARAGWQSALNYSITGRGQYYRDGIGSAVTT
                     DTYVCGLGVSGAVPNPWVRGVNAGLDAYRQQAESDPAKTIIHALSANLTSQLPAGWTS
                     RLSANIQDTHVVYGISADNRTQQYGFDLGHGLALAGFSGRANAGIVYSILRQTGQNSD
                     DLGLNFSSLLSRGPLSFSLSYRHLNQDRQTGLVDNLTHGLNAACEYAYKRHQLRLEAD
                     YGYRKPQNSDESADTRIALTWTFSFDRPAAPVKAAPAEAPPAEAAKAAPAPPEIAPDV
                     QPPIRSLADLSPGMPMDRALAGLEKRNIKGGTREGRNLVFETVWFDDVSQRQRLVLTE
                     QMKKLERTAVIIDLNQVGELDNAYQLYERIRTRLNREFGAATTQEVGAFSPSLAQDLR
                     DGKFIRISDWKTPAGVLRLGIPRRLDGIVRIEAQHAQSFPMSGYVSWSVEEIQ"
     gene            complement(2593..3087)
                     /locus_tag="PLR47_02255"
     CDS             complement(2593..3087)
                     /locus_tag="PLR47_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11788.1"
                     /translation="MKKYLMVFILLLIALPGQAQEMRQWSPIRSKAHATAKLPPGAKP
                     VEPSQPLPSGTLARAAQEIAASWNSPDFKKLVSGNFYDQQRLTDAMNTETAKDARLKV
                     LNSHSASMLNQTIVPDPQGGHLRVSNVSVILETRAEFNDPKQGFISAPGTNEMVMEVV
                     EKID"
     gene            complement(3084..4058)
                     /locus_tag="PLR47_02260"
     CDS             complement(3084..4058)
                     /locus_tag="PLR47_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11789.1"
                     /translation="MGQQLMNRRSAFFTSALLMLLLLPCFAVFEAPAAGLGSENVLGR
                     DTQPVFSPEMKRQLQDRQRPAAQMTAEGKLSPGTWTVKILENRVISGHNFVNTAQPLY
                     NFTLNGVTYTFDRTQVADPALLLKTAVTIQTKGAGSLNLNDPETLSAVKQLQQMSNGT
                     LHIVSETPSPGPGPGPGPAPGPPVACMPAIYPLNGNVGTWIFLRLETLPASCAIDQNP
                     AGAPIIGIQNPHTSCSDAGSKVFFDVISGEPITPGAGFSTYTGVCGSGLRAVEASSIN
                     NQFIIQIGATRYQVTFSAGNGGLQPVPYSHFGRYNSITVYDASVRALP"
     gene            complement(4043..6964)
                     /locus_tag="PLR47_02265"
     CDS             complement(4043..6964)
                     /locus_tag="PLR47_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11790.1"
                     /translation="MKQLSVNIKILIALMLIIGSGALMMPPRSLAQQNQAPAQVKEES
                     PQEFVDRNLAAARKRLAAARSRYRQSRGPADLAQSPIDPSVILQSLPRPRKLKFSFRQ
                     PEKIEEIKPAPTVPGTWTFYPGYLSFSGAGNQSGFSANAATDELTSALLRLPRNLPLA
                     ATASIIGVNSQSHPVEKCCLEYKILKNNDGSFAGSQCIRTAQEKYISVQQNIDPRAWS
                     DRIGFRLTTSEGKILGNGAAVRFDQPGPAAVTATLTGQYKAEEFTPQNKQYCQVFDYA
                     TELIDNSKPARYRLNIQNFNVTDRWHNPGDKTVITGDWKIPQNKGEQKQKIDVEVIAV
                     KGLHLDGKDYTKTRSEADGYDLFARENEDFKGVYFRNDKKAPRLILQRAWPFDSVKID
                     GSRGGFREVLEPVSKWPTALTYAIEGGGPFAVSQDGDVQPLGSIGEARLNIQLGQHWS
                     LFRKLTANRWQAVLDNALADGAVSPGGSYSVALQVQGPADMAKYKVKWKGGQWKNPQA
                     KFEKMGNAWQAQNVVAVPGGTRMGTVLQLDVEVTVGDAGDEDVRLSWSRRLNVVPSVA
                     SLELTLAAKCRPASRDGVDLFFPNYHPDGNRFAALPIYRDDKGNELAPETIKRFLTGA
                     SLRLKSDTPAVAMADGNGGQTGRTAGEAWISAELGGIDLDPRGQFETAKGAMLASNQV
                     KVTANQLILSRGKASGGFTPYVLRVIGPAAMDQYQALFHFEGGSTGTPFIQGEDGGYS
                     AGLSAVAAVKKVEILKGGKTVAMLPFSDNVVVAQAGIRLMPVTVPGRVIDTIPLVDAG
                     SLTTQTDCRRQLRTQYPGEYGNLSDAALDEVCDTIREAEKKEIKEQRATQKEQKKILK
                     SLKNEGRQLMVFGDSTQLGAAVTGHFDPDRMHCRWRLDQGGSLKFEQAQSPITRVGGD
                     GACFNDVKGFQGNFDTEATATVELIYAHPAAGQGQFIPGGTQIMRADVDAKQVEEWVN
                     N"
     gene            complement(6961..9972)
                     /locus_tag="PLR47_02270"
     CDS             complement(6961..9972)
                     /locus_tag="PLR47_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11791.1"
                     /translation="MTKLFSRIFFILLLIAFAQFVPSGAAANAELEEIRRLETLLLTP
                     CDKPLAYMTELVRLGQTYQDQERQRLALYRGILDTHRLVLANFLTDNADKAGATAGPF
                     RARYDQLYQEADREGMLREASYEDVPEYMNMADMAVRVWQYDADQDRRYYGSEASVGR
                     WAKKQEDLGNKLFFVEAQRAGAEHLRKEMIRLKEDWKTARAEYFKTMEADPYKDHFSE
                     LDRRAAFMTDTLRREYESNRQALTIAAQSGSYEHCLGEIVESSTYTAFSENSGFSPPP
                     TIYQAGDAGTVEALPGMGFRMPKPAVFDRIKAAQTDIPEEFPLDLPLKFDPLPYIKTE
                     IECQKIKIKAEKDAIIAMGTYLTGLKGTGGLLVGEGFNVFHGVCEGFGSVGNILLGIG
                     YAQLHPIEAYEKLNKTKNDMVYLWESSDAKQALIEKGVGSIADYFRGIGSGFNLLLID
                     DPPDGVYDRQTMEDWRQRAEETQLARKMLTNTEMLVGSILADVATGVILSRGSQAAKL
                     AAEAADTAGDLSQAAARAKKLEEAAARAGKTLDDVPSVKTVEHLADDIRKNQDALMKL
                     ERDLSGKTRDIPYDPKMLRTPEDLLADASTVDTKGGMNAYVRLNEDLGMKVSNQPIIK
                     GDTPAAVLSRKSRDLDDAGRVYLQDITKDSSIGRLPQVKDRYYIVQDASGAAKTVRNL
                     AEIPDGASYKVVDVMEHIPSSMHADKLAGAQKLTADHVRAYEAFMRDMNAKGYVWTDN
                     KLSNFAFETVDEAKGVYRVVPLDTGGFYKVGDLPSGVNMSKADFARKMQRAYDKGVEA
                     NVEGAPGKMLAVSQNMVDEGIPESMLDQVDDIMGVRKINPQAEQMVNTWDSGYRMSSP
                     KNPQGQAYSHAGDYARESGKADDVLTREVSEAAEASQQARKLEYAKTRASLDAQKVRL
                     EQINSQLDAGAQKLVQADIVEKSAAATAARTSVNTAREGVQTVAGIQAAQERILCLEI
                     KEKILSGIQADWITEGWKNCQKLGLTFEGGR"
     gene            complement(9983..10615)
                     /locus_tag="PLR47_02275"
     CDS             complement(9983..10615)
                     /locus_tag="PLR47_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11792.1"
                     /translation="MLIKAPAESAFVELNSSYVPPMTIEGWACGTSGAEAGQKTVDLV
                     YRLRLAEQGFKNASAGLKNSYENRKKTLKAPAVFQGLSLPEVTATMAVSLNRFPKNIG
                     EFNADLNRLQKDLNQSLKEKPDRFFGVDISKRMLSESHLADRLRLETWQDDGAYQVWS
                     AIDCYSVADLQSMQSSISQALSAALTARDELIKTAAQVHDGQGAAFTLPR"
     gene            10987..12285
                     /locus_tag="PLR47_02280"
     CDS             10987..12285
                     /locus_tag="PLR47_02280"
                     /inference="COORDINATES: protein motif:HMM:NF016915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11793.1"
                     /translation="MERLWKGCLLCLAVLLFAGCTAVNHYLGFWQAKQEAREAFSREQ
                     SAELLMQLAPEDAYLLLGKLAFDREPAGPILLLALTDKFKKREIAARSPMQTPLDYYQ
                     IHLPEGVYEIYFFADLDENGYFEAHECVGRLPRPVTIAKQKVKDGLSVIGPDLKLNLA
                     APSKTDLVFEEKVRRHSYVYESLEDEFFDPRYGEIGLYDFKVFMAHTQQYIFSFETMN
                     PRKTQVLFVHGVAGTPRDFQYMVRGIDRKRYQPWFYFYPSGLPLKKLGANLANIIKYA
                     EANNPGRLRRLIIVAHSMGGLVALSTIQHLSADGVPPYLKGCISFNSPYGGVEAAASG
                     IKYAPAVVPSWHDVAAGSPFLNELYTEGKHKAIPFYLFFGYKTGDSSDGTITLQSQLN
                     RNIHLSAHKSYGFNASHVGILRDEQARQVFYRTLSDLDRD"
     gene            complement(12415..>14523)
                     /locus_tag="PLR47_02285"
     CDS             complement(12415..>14523)
                     /locus_tag="PLR47_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11794.1"
                     /translation="EGALQRAANAVLPDWQLSGSNTFRLEYYDYQGDEAQAPYSYEGL
                     TSYDEIGLNFSRRKNPYDLWKGDIFGVINGSPYRSADYGLVPERIHLMREKGDIALPF
                     RVELGDVYSYYTYRTLQTSLKGVQVDLQPFAGSASRHSVLLSSGARLSSWRHLNFRDS
                     YFNGGSYLIENPTFGRYSLNIAYNTRQSDEARNILQRTQMVYSLTGQHTLQAKNQKLL
                     LEGELGLFRGDHDGAADAQSGQNKSDRGIFFQASGSGKHPFTYRLRYEEYGEDYQPTG
                     ASITPALRNLEGHAGWLFARGYQLRLRAQGRRNDLGRANPTDASLFGANLTGPLGSLD
                     VFRQDAKNRTDSIDNLTHNVNLNVNIPKIAGWTGRLGYQYLNFFNRLPDAGDSASHQF
                     SLSADHLLDFLGCRGAVTPGVVLRKINSDTGDSFDVSPTIAFRLTRGPHSLDYTLSRT
                     DQRRYVTDGIDMITTTQALNYRFTLNNHSFGLELSSGYQNPDPGQTIDYYKLAAFWTY
                     SFDKPAKTPGRGKAAEERREIPEMPAKAFDLASFAPGAKMDEAEKQLIAKGIVNPVRQ
                     ENFLLYETRVFDEIDLRQRLVLIQKNNALNRSAVIIDFAYLEQPDDIMQTFQRILSIL
                     LDRYGNPSNFYEKGETGGRLLSDIRNGRFIRLTEWSRPGGVIRFGMPRRLDGQVRMEI
                     QFAESFPPLSETLWSLEEIK"
BASE COUNT         2916 a         4091 c         4146 g         3370 t
ORIGIN      
        1 gcacgtgaat gccgggagac tggatgtccg tatccacgac gccgacgcgc cggccttttc
       61 gggccagaag gcaggccagg ttggccgtgg tgttggtttt tcccgttccg cctcgaaagg
      121 aatgaatcgc gataatttca ggcatggcgt cctctttgcc gaagtcggcc gcagtataga
      181 aaaagcattg tgccaagtca aggatatcat gtttcaaaaa gttcctccgg acggccaagg
      241 gatcggtttg ctcggcgagg atcgtcagaa gccggatctt cgggcggcgg ccgtcgcatc
      301 gtcagtattg cggctgatcc gttgggctct tttgacggag gcgcacgata ggcaatttat
      361 ttcggcctgt caacccgatc cgggagcatc ggtcactgta tttcctctac gctccaggaa
      421 acatagccgg acatcgggaa ggattgagcg tgctgcgctt cgattcggac aatgccgtcc
      481 agacggcgtg gaatgcccag gcgcaatacg ccggcggggg ttttccagtc gctgatgcgg
      541 atgaattttc cgtcgcgcag atcctgggcc agactggggc tgaacgcacc cacttcctgt
      601 gtggtcgcgg cgccgaactc gcggttcagc cgcgttcgta tccgttcata caactggtag
      661 gcgttgtcca gttctcccac ctggttcaga tcaatgatga cggcggtccg ctccagcttc
      721 ttcatctgct cggtcagcac cagcctctgg cgctgggaaa catcgtcaaa ccagacggtt
      781 tcaaaaacga gattccgccc ttcgcgggtt ccccccttga tgtttctttt ttccaggccc
      841 gccaaagccc ggtccatggg catgccgggg ctgagatcag ccagcgaccg gatgggcggc
      901 tggacgtccg gcgcaatttc cggcggagca ggggcggcct tcgccgcttc cgcgggcggg
      961 gcctctgcgg gcgccgcctt tacgggcgcc gccggccggt caaagctgaa ggtccaggtc
     1021 agggcaatcc gggtatccgc gctctcgtcc gagttctgag gtttgcggta gccgtaatcc
     1081 gcttccaggc gaagttgatg ccgtttgtag gcatattcac aggcggcgtt gaggccgtgg
     1141 gtcagattgt ccaccagtcc ggtctggcga tcctgattca gatgccggta gctcaggctg
     1201 aaactcaggg ggccccggct cagtaaggag gaaaaattca atcccagatc atcggagttc
     1261 tggccggtct ggcgcaaaat gctgtaaacg attccggcgt tggcccggcc tgaaaaaccg
     1321 gccagcgcca gaccatgccc caaatcaaat ccgtattgct gggtgcggtt gtcggcggat
     1381 atgccgtaga ccacatgggt gtcctgaata ttggccgaaa gacgggatgt ccagccggcc
     1441 ggcagctgac tggtcagatt ggcggaaagc gcatggatga tggtcttggc cgggtcggat
     1501 tcggcctgct gacggtaggc gtccaggccg gcattgacgc ccctgaccca gggattgggc
     1561 acggcgccgc tcacgcccag gccgcagacg tacgtatccg tggtgaccgc cgaaccgatg
     1621 ccgtcgcgat agtattgacc ccggccggta atcgaatagt tcaaggcgga ttgccacccg
     1681 gcgcgggcgt ctatggtgcg ccgcccggcg ctgacgacgc ccccgcgggg aatataggct
     1741 ttgtcgtatt cttcatagcg caggctgtag gtcaggggaa ttttgccgcg tccctgaaac
     1801 tgggcaaacg cgcccagccc ttcatccttg aaggattcgc cgttttgggc gttgtccccc
     1861 cgaagataat tcagctcggc atccagcgac aactgctggg ccagaaaatt gaaattgtga
     1921 ttgacggcca gcccggtcac atactggcgc aaatcgggag tgccggattg cggcgcctga
     1981 tcgttgtagg caaaattcaa cgacgctctt gtccggctcc agttcatcat ccaggaggcc
     2041 ccgccaaaaa gattgtccgc cagacggttg tcgaaaaaat cccgggacgc gccgccgccc
     2101 tggagcagca gggagttttg cgctcccaaa aactggccgg gctgcagctc aagctgggcg
     2161 cccttgagcg aacgctgaag aacgcggggg gtttgaaagc cgtgaaagtc gcccaggtcc
     2221 atccggtagg gaacgggcgc ttctccattt tcctggttga tgttgaagcg ctccaggatc
     2281 acgccgtact ggggggagcg gtagggcgat ccgctgatgg cgccggacgc gtagcctttg
     2341 atggtattgt aggggttttg aatgttttga aaccggatat tgagatcgtt gtaaggcctc
     2401 cagccttcct gaaggtaggc gccgtccggc ccgtgggaat tgtagtagtc caggtgcatg
     2461 ctgttctggc cggaaacgga ccagccggga aaaatggcct ggacgtcaac ggcaaaagcc
     2521 gccccgggcg ggctgcacag catcaggccc agcagaaaac agatgatttt tgatttccgg
     2581 aagatggatc cgctaatcaa ttttttccac gacctccatc accatttcat tggtgcccgg
     2641 cgcggaaatg aatccctgct tcggatcgtt gaactccgcg cgtgtttcca gaatgaccga
     2701 cacgttgctg acccgcagat gcccgccttg cggatcggga acaatcgttt gattcaacat
     2761 ggaggcgctg tggctgttga gaactttcag ccgggcgtct ttggcggttt cggtgttcat
     2821 ggcgtccgtc aggcgctgct gatcgtagaa gttcccggaa acgagttttt tgaagtccgg
     2881 actgttccag gatgcggcaa tctcctgagc ggcgcgggcc agagttcccg aaggcagcgg
     2941 ctgcgacggt tccaccggct ttgcgccggg cggcagtttt gccgttgcat gggccttgct
     3001 gcggatggga gaccattgcc tcatttcctg ggcctgtccg ggcagggcga tcagcaaaag
     3061 gatgaagacc attaagtatt ttttcatggc agggccctca cggatgcgtc gtaaacggta
     3121 atggaattat agcgtccgaa atgactgtag ggaacgggct gcaatccgcc gttccccgcc
     3181 gaaaatgtca cctgatagcg ggttgcgccg atctggatga tgaactggtt gttgatggag
     3241 gatgcttcca ccgcccgcag accgctgccg cacacgcctg tgtacgtgga aaatccggcg
     3301 ccgggcgtaa tcggttcgcc gctgatcacg tcaaagaaca ccttgctgcc ggcatccgaa
     3361 caggaggtat ggggattctg aatgccgatg atcggcgctc cggcgggatt ctggtcaatg
     3421 gcgcagcttg ccggaagcgt ctccagccgc aggaaaatcc aggttccgac atttccgttc
     3481 agaggataaa tcgccggcat gcaggccacc ggcggcccgg gcgcgggacc ggggccgggc
     3541 ccaggcccgg gagacggtgt ttccgatacg atatggagcg ttccgttgct catctgctgc
     3601 aattgcttca ccgccgacag agtttcgggg tcattgaggt ttagcgaacc tgcgcctttt
     3661 gtctgaatgg tgacggctgt ttttaaaagc agcgccggat cggccacctg tgttctgtcg
     3721 aaagtatacg tgactccgtt gagagtaaaa ttgtaaagcg gctgggccgt attcacgaag
     3781 ttgtggccgg agatcacccg gttttccaga attttgactg tccacgttcc ggggctgagc
     3841 ttgccttcgg ccgtcatctg cgccgcgggc cgctgccggt cctgaagctg ccgtttcatc
     3901 tcgggggaaa agacgggctg cgtgtcccga ccaagaacat tttccgagcc gagtcccgcc
     3961 gccggcgctt cgaaaacggc aaagcacggc aaaagcaaaa gcatcagcag cgccgacgta
     4021 aaaaaagcac tgcgtcgatt catcagttgt tgacccattc ttccacctgt ttggcgtcca
     4081 catcggcgcg catgatctgt gtgccgcccg gtataaactg accctggccc gccgccggat
     4141 gagcgtaaat caattccacc gtagccgtgg cttccgtgtc gaaatttccc tgaaatccct
     4201 ttacgtcatt aaaacatgcg ccgtcgccgc ccacgcgggt gatcggactt tgcgcctgct
     4261 cgaatttgag cgatccgccc tgatccagcc gccagcggca gtgcatccgg tccggatcga
     4321 aatgcccggt aacggcggcg cccaactgcg tggaatcgcc gaacaccatg agctgacggc
     4381 cttcattttt caggcttttg agaatttttt tctgttcttt ctgggttgcc cgctgctctt
     4441 tgatttcttt cttttccgcc tcgcggatcg tatcgcaaac ctcatccagg gcagcatccg
     4501 aaaggttccc gtattcgccg ggatactgag tgcgcagctg cctgcggcag tccgtttgcg
     4561 tggtcaggct tcccgcgtcc acaagcggga tggtgtcgat cacccggccc ggcacggtga
     4621 ccggcatcag ccggatgcct gcctgagcaa caaccacgtt gtcggagaaa ggcagcatgg
     4681 ccactgtttt gccgcctttg agaatttcca cttttttaac ggcggcaacg gcggacaatc
     4741 cggccgaata gcctccatcc tcgccctgaa taaaaggcgt tccggtgctg ccgccttcaa
     4801 aatggaagag cgcctgatat tgatccatgg ccgccggacc gatgactctc agaacataag
     4861 gggtgaagcc gccggaggct tttccccgcg aaaggataag ctgattggcc gtcactttaa
     4921 cttgattgga agccagcatg gcccctttgg cggtttcaaa ctgtccgcgc gggtccagat
     4981 cgatgccgcc caactcggcg ctgatccagg cttctcccgc cgttcgtccc gtctggccgc
     5041 cgtttccgtc cgccatggcc accgccggag tgtcgctctt caaacgcaaa gaagccccgg
     5101 taagaaaacg tttgatggtt tccggcgcca gctcgttgcc tttatcgtcc cgatagatcg
     5161 gcagagccgc aaaccggttg ccgtcgggat gataattggg aaagaacaaa tcgacgccgt
     5221 cgcgggaagc gggtctgcac ttggcggcga gcgtcaactc cagggaggcg acgctgggga
     5281 cgacattcaa ccgccgggac caggaaagcc ggacatcttc atcgccagcg tcaccgaccg
     5341 tcacctccac atccagttga agaaccgttc ccatcctggt tcccccggga acggcgacaa
     5401 cattttgcgc ctgccaggca ttgcccattt tttcaaattt agcttgcgga tttttccact
     5461 ggccgccttt ccatttcact ttgtacttgg ccatatcggc cgggccctgc acctgcagcg
     5521 ccaccgaata gcttccccct ggcgaaaccg cgccgtcggc cagagcatta tccaaaacag
     5581 cctgccagcg attggcggtg agcttgcgga agagagacca atgctgaccc agttgaatgt
     5641 tcaagcgcgc ctcgccgatg ctgcccagcg gctgcacatc accgtcctgg ctgaccgcaa
     5701 acggtccgcc gccttcgatg gcgtaagtca gtgcggttgg ccatttcgag accggttcca
     5761 gaacttctct gaaaccgccc cggctgccgt cgattttgac gctgtcaaac ggccacgccc
     5821 gctgcagaat cagacggggg gcctttttgt cgttcctaaa gtacacccct ttaaaatctt
     5881 cgttttccct ggcaaaaaga tcataaccat ccgcttcgct gcgagttttc gtgtaatcct
     5941 tgccgtccag atggaggccc ttgaccgcga tcacttccac atcaattttc tgtttttgtt
     6001 cgcccttgtt ttgcggaatt ttccagtcgc cggtaatgac cgttttgtcg ccgggattat
     6061 gccagcgatc ggtgacgttg aagttttgaa tgttcaggcg ataacgggcc ggtttggaat
     6121 tgtctatgag ttccgtcgca taatcgaaaa cctgacaata ttgtttgttc tggggcgtga
     6181 attcctccgc cttgtattga ccggtgagcg ttgccgtgac tgcggccggg ccgggttgat
     6241 caaaccgcac ggcagcgccg ttgcccagaa tttttccttc gcttgtggtc agacgaaagc
     6301 cgatccggtc ggaccaggcg cgcggatcaa tgttttgctg aacggagatg tatttttcct
     6361 gagccgtgcg gatgcattgg gaaccggcaa aggagccgtc gttatttttc aaaattttgt
     6421 attccaggca acacttctcg acagggtgcg actggctgtt gacgccgatg atcgaagcgg
     6481 ttgcggccaa aggaagattt cgcggcagac gcagcagggc ggaggtgagc tcatccgtcg
     6541 ccgcgttggc ggaaaagccg gactggttgc ctgcaccgga gaagcttaaa taccccggat
     6601 aaaaggtcca ggtaccgggc acggtaggcg ccggtttgat ttcttcaatt ttttccggct
     6661 gacgaaagct gaacttcagt ttccggggcc tgggcagaga ttggagtatg accgacggat
     6721 cgatggggct ctgagccaga tcggccggtc cgcggctctg ccggtagcgg cttcgggcgg
     6781 cggccaggcg cttgcgggcg gcagccaggt tacgatcgac aaactcctgc ggcgactctt
     6841 cttttacctg ggcgggggcc tgattctgct gggccaaaga gcgcggcggc atcatcagcg
     6901 cgcctgaccc gataatcaac attaaagcaa ttaatatttt gatattgaca gataattgct
     6961 tcatcggcca ccttcaaacg tgagtcccag cttctgacaa tttttccatc cttcggtaat
     7021 ccagtcggcc tgaatgccgg acagaatctt ttccttgatc tccagacaga ggattctttc
     7081 ctgggcggcc tgaattccgg caaccgtctg cacgccttcc cgggccgtat tgacggatgt
     7141 ccgggccgcg gtcgcagcgg cggacttctc gacaatatcc gcctgaacaa gcttttgcgc
     7201 ccctgcatcc agttgactgt tgatttgttc cagccggact ttctgggcat ccagagaagc
     7261 ccgggttttg gcgtattcaa gtttcctggc ctgctggctg gcttcagcgg cctcggaaac
     7321 ctcccgggta aggacatcat cggcttttcc cgattcccgg gcgtagtccc cggcatggga
     7381 ataggcttga ccctggggat ttttgggtga ggacatgcga taaccgctgt cccacgtatt
     7441 gaccatctgc tccgcctgcg gattgatttt tcttacgccc atgatgtcgt cgacctgatc
     7501 cagcatgctt tcaggaattc cttcatccac catattctgg gaaacggcta acatcttgcc
     7561 gggggcgccc tcaacatttg cttcgactcc tttgtcgtat gcgcgctgca tcttccgggc
     7621 gaaatcggct ttggacatgt tgacgcccga cgggagatcc ccgactttat agaagccgcc
     7681 cgtatccagc ggcacgacgc gatagacgcc cttggcctca tccaccgtct caaaggcgaa
     7741 gttgctcagt ttgttgtccg tccagacata gcctttcgcg ttcatgtccc tcataaaggc
     7801 ctcgtaggcc cggacatgat cggcggtcag cttttgcgcc ccggccagct tgtctgcgtg
     7861 catggaggag ggtatgtgtt ccatgacatc cacaaccttg taactggcgc cgtcggggat
     7921 ctcagccagg ttgcgcacgg tttttgccgc accggatgcg tcctgcacga tgtaataccg
     7981 atccttcacc tgcggaaggc gtccgatcga tgaatctttt gttatatcct gaagatagac
     8041 tcgtccggca tcgtcgagat cacgggattt gcggctcaaa accgccgccg gagtgtcgcc
     8101 cttgatgatc ggctggttgc tcactttcat tcccagatct tcgttgagcc gcacgtaggc
     8161 gttcattcct cccttggtgt caactgttga cgcatcggcc agcaggtctt ccggcgtgcg
     8221 cagcattttg gggtcgtagg ggatatccct cgtcttgccg gataaatccc tttccagctt
     8281 catcagcgca tcttgatttt ttctgatgtc gtcggccaga tgttcgacgg tcttgacact
     8341 gggaacatca tctaaagtct tgcccgcccg ggcggccgct tcctcaagct ttttggctct
     8401 ggcggcggcc tgcgacagat cccccgccgt gtccgccgcc tcggcggcca gttttgcggc
     8461 ctgtgaaccc ctggacagga tgacgcccgt ggcgacgtcg gccagaatgc tgcccacgag
     8521 catttccgtg ttggtcagca tcttccgggc caactgcgtt tcttccgctc tctgccgcca
     8581 gtcttccatc gtctggcggt cataaactcc atccggcgga tcatcgataa gcagcaggtt
     8641 gaagccggag ccgattcccc ggaaataatc ggcaatggaa ccgactccct tttcaatcag
     8701 tgcctgcttg gcatccgaac tttcccagag gtaaaccatg tcattcttgg ttttattgag
     8761 tttttcatag gcttcaatcg gatgcagttg ggcgtagccg atgccgagca atatattgcc
     8821 gacagacccg aaaccttcgc agacgccgtg aaatacattg aagccttctc cgacaaggag
     8881 tccgcccgtc cctttcagac cggtcaggta ggttcccatg gcgatgatgg cgtctttttc
     8941 ggcctttatc ttgattttct ggcattcgat ttccgtcttg atgtagggca gcggatcaaa
     9001 tttcagcggc aggtccaggg gaaattcttc ggggatatcg gtctgcgccg ccttgatcct
     9061 gtcgaaaaca gcgggtttgg gcatgcggaa acccatgccg ggcagagcct ccaccgttcc
     9121 ggcgtcgccc gcctgatata ttgtcggcgg gggagaaaaa ccggaatttt cggaaaaggc
     9181 cgtgtatgtg gagctctcca cgatttcgcc caggcagtgc tcatagctgc cgctttgcgc
     9241 cgcgatggtc aaagcctggc ggttgctttc atattccctg cgcagcgtgt cggtcatgaa
     9301 agcagcccgg cggtccagtt ccgaaaagtg atccttgtag ggatcggctt ccatggtttt
     9361 aaagtactcc gcccgcgcgg ttttccaatc ctctttgagc cgaatcattt ctttgcgcag
     9421 atgctcggcg ccggcgcgct gtgcttcaac gaaaaacagc ttattgccga gatcttcctg
     9481 cttttttgcc caacggccga cgctggcctc gctgccgtaa tatcgcctgt cttgatcggc
     9541 gtcatactgc cacacgcgaa cggccatgtc cgccatgttc atatattcgg ggacatcttc
     9601 ataagatgct tccctgagca tcccctcgcg gtcggcctcc tggtaaagct gatcatagcg
     9661 cgcgcgaaag gggccggcgg ttgcgcctgc cttatcggca ttgtccgtca gaaaattggc
     9721 caggaccagg cggtgggtat ccagaatgcc gcggtagagc gccaggcgct gtcgttcctg
     9781 atcctggtaa gtctgtccca ggcgcaccag ctccgtcatg tacgccagcg gcttgtcgca
     9841 gggggtcaac agcagcgttt ccagacggcg gatttcttcc agctcggcgt tcgccgccgc
     9901 ccctgaagga acgaactgcg caaaggcaat caagagcaaa atgaagaaga ttctggaaaa
     9961 aagtttcgtc atgatcagtg ccttatcgcg gcagcgtaaa agccgccccc tgtccgtcgt
    10021 ggacttgtgc cgccgtcttg atcagttcgt cgcgggccgt caaagcggcc gacagcgcct
    10081 ggctgatcga actttgcatg gattgaagat ccgcgacgga atagcagtca atcgcgctcc
    10141 agacctggta ggcgccgtca tcctgccagg tttccagacg cagtctgtcg gccagatggc
    10201 tttcggaaag catccgtttg gaaatatcaa caccgaagaa acgatcgggc ttttctttta
    10261 aagattggtt taggtctttt tgcaggcggt tgaggtccgc gttgaattcg ccgatatttt
    10321 tgggaaaccg gttgagggaa acggccatgg tggcggtaac ttcgggaaga gaaagcccct
    10381 ggaagacggc cggcgccttg agagtttttt tgcggttttc gtaggagttt tttaatccgg
    10441 cggaagcgtt tttaaatccc tgttcggcca gacgcaggcg atacaccaga tcgacggttt
    10501 tttgtcccgc ttcggcgccc gacgttccgc aggcccagcc ctcgatggtc atgggtggaa
    10561 cataggaaga gttgagctcg acaaacgccg attccgccgg cgctttaatc agcaagtctt
    10621 caccggcccc gaccggcgca gcggcggcca gcagaatgag aataggcagg aaaggaaata
    10681 agaccggaaa agttcttttt ccggcccgaa acggaatgga gaaaatttca gcaagcgaag
    10741 tacacatcaa ccgtaagaat gtgcttaaat gccttttatg actttggcaa cctgattgtc
    10801 catgcaaccg tgcaaccgcc ggaaccggga aaaaaccatt ccctcgcttg gcatcgcatt
    10861 aaatcatcgg attttggaaa tcaagtgttt ttttcaaggt gattttgctg aattgacagg
    10921 cggactgatt ttatttatgg ttcattcggt tttcaaaaat catggacatc aacatcagga
    10981 gaatctatgg aacgcttatg gaaaggatgt ttgctctgcc tggccgttct tctttttgcc
    11041 ggctgcacgg cggtgaatca ttatctcggg ttctggcagg ctaagcagga ggccagggag
    11101 gccttcagcc gggagcagag cgcggagctg ctgatgcagc ttgcgccgga ggatgcctat
    11161 cttcttctgg gcaagctcgc ttttgaccgg gaacccgcgg gtcccatcct tctgctggcg
    11221 cttacggaca aatttaaaaa aagagaaatc gccgcccgct ctcccatgca gacgcccctg
    11281 gattactatc agatccattt gcccgaaggt gtttatgaaa tttatttctt cgccgacctc
    11341 gatgaaaacg gttatttcga agcgcatgaa tgcgtgggcc gcctgcctcg gccggtcacg
    11401 attgcaaaac aaaaagtcaa agacggattg tccgtcatcg gaccggattt aaaactcaat
    11461 ctggccgctc cttcgaaaac cgatctggtt ttcgaagaaa aggtccggcg gcattcctat
    11521 gtttacgaat cgctggagga tgaattcttc gatccccgct acggggagat cggcctttat
    11581 gatttcaagg ttttcatggc gcatactcag cagtatattt tttcctttga gacgatgaat
    11641 cctcgaaaga cgcaggtgct ctttgtgcac ggcgtcgcgg gcacgccgag ggattttcaa
    11701 tacatggtcc gcggaatcga ccggaagcgc taccagccct ggttttattt ttatccttcc
    11761 ggcctgccct tgaaaaaact gggcgccaac ctggccaaca tcatcaagta tgccgaagcg
    11821 aacaatcccg gccgcctgcg ccgcctcatc atcgtcgcgc acagcatggg agggctggtg
    11881 gctctatcca ccattcagca tttgtccgct gacggcgttc cgccctatct caagggctgc
    11941 atctccttca actctcccta cggcggcgtc gaggcggcgg cgtccggcat taagtatgcg
    12001 cccgccgtcg tgccttcctg gcatgacgtg gcggcgggaa gcccctttct gaacgagctt
    12061 tacacggaag ggaagcataa ggccattccc ttctacctgt ttttcggtta caagaccggc
    12121 gacagcagcg acggaaccat cacgctgcaa agccaactga acaggaatat ccatttatcc
    12181 gcgcataaaa gttacggttt caatgcctcg catgtgggca tactccgtga tgaacaggcg
    12241 cgtcaggtgt tttacaggac cttgtccgat ctcgaccgtg actgacggca ggggggcatg
    12301 aggcatcgcc ccgggcgctt catgatgttc aggccttccc cgtcttgttc cgcatcggcg
    12361 cagtccggac ccattcccac gctccagcaa caggagccgg gcgcgatcat ccggctattt
    12421 tatttcctcc agactccaca gggtttcgct caaaggcggg aagctttccg caaactgaat
    12481 ctccatgcgt acctggccgt caagccgccg gggcatgccg aaacggatca cgccgccggg
    12541 gcgcgaccac tcggtcagac gaataaaacg gccgtttcga atatcggaca aaagccggcc
    12601 tcccgtctct cccttttcat aaaaattgga cgggttgccg tagcgatcca gaagaatgga
    12661 cagaatcctc tgaaacgtct gcataatatc atcaggctgc tccaggtagg caaaatcgat
    12721 gatgacggcg gaccggttca atgcattgtt tttttgaatc aagaccagcc gctgacgcag
    12781 gtctatttca tcaaacactc tggtttcgta aaggagaaag ttttcctggc gcaccgggtt
    12841 gacaatgcct ttggcgatta attgcttttc ggcctcgtcc atttttgcgc ccggcgcgaa
    12901 ggaagccaaa tcaaatgctt tggccggcat ttcagggatt tctcttcgtt cttccgccgc
    12961 tttgccgcgc cccggagttt ttgccggttt atcaaaagaa taagtccaga atgccgccag
    13021 tttataataa tcgatggtct ggcctggatc gggattttgg tagccggagg aaagttccag
    13081 accaaaggaa tggttgttca gggtaaagcg gtagttcagg gcctgggtgg tagtgatcat
    13141 atcgatgccg tcggtgacat agcggcgctg atccgtccgg ctcagggtgt aatccaggct
    13201 gtgcggcccc cgcgtcagcc ggaaggcaat agtgggagaa acgtcaaagg aatctccggt
    13261 gtcgctgttg atttttctga gcacgacacc cggcgtgacc gcgccccggc aacccaaaaa
    13321 atcaagcaaa tgatccgcgc ttagcgaaaa ttgatgggaa gcgctgtccc ccgcatcggg
    13381 aagacggttg aaaaaattga gatactggta gcccagccgt cccgtccagc cggcaatttt
    13441 gggaatattg acattcagat ttacattgtg tgttagattg tcgattgaat ccgtccggtt
    13501 tttcgcatcc tggcggaaga catccagtga tcccaaaggc ccggtaagat tcgcgccgaa
    13561 aagagacgca tccgtcggat tggcgcggcc gagatcattt cttctgccct gggcccgcag
    13621 ccgcagttga taccccctcg caaaaagcca gcccgcatgt ccttccaggt tgcgcaaggc
    13681 cggtgtaatg gacgcgcctg tcggctggta gtcttcgccg tattcttcat agcgcagccg
    13741 gtaagtgaat ggatgtttgc cggagccgga cgcctgaaaa aaaatccccc tgtcgctttt
    13801 gttctggccg ctttgcgcat ccgctgcgcc gtcatgatct cctcgaaaca gtcccagctc
    13861 gccttccaga agcagtttct ggtttttcgc ctgaagcgta tgctggccgg ttaagctgta
    13921 aaccatttga gtgcgctgga gaatgttgcg ggcttcgtcg ctttgacgcg tgttgtatgc
    13981 aatattcaga ctgtagcggc caaacgtcgg gttttcgatt aaataggagc cgccgttgaa
    14041 atagctgtcg cggaaattca gatggcgcca ggaggaaaga cgggcgccgg aggataaaag
    14101 cacggaatgc ctggaggccg agccggcaaa aggctgcaga tcaacctgaa cgcccttgag
    14161 agacgtctgc agggttctgt aggtgtagta ggaatacaca tcgcccagtt ccacacggaa
    14221 aggcagagca atgtctcctt tttccctcat gagatggatg cgttccggaa ccagtccgta
    14281 atcggcgctg cggtagggag acccgttgat cacgccaaat atatccccct tccagagatc
    14341 ataggggttt ttgcgcctgc tgaaattgag gccgatttca tcataggagg tcagtccttc
    14401 ataactgtaa ggggcctggg cttcatcccc ttgataatcg tagtattcca gccgaaaagt
    14461 gttgctgccg gataattgcc agtccggaag aacggcattt gccgctcttt gcagcgcgcc
    14521 ttc
//
LOCUS       DAOSIM010000026        14395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_4299_length_14395_cov_9.359205, whole genome shotgun sequence.
ACCESSION   DAOSIM010000026 DAOSIM010000000
VERSION     DAOSIM010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 14395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14395
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4299_length_14395_cov_9.359205"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="PLR47_02290"
     CDS             <1..852
                     /locus_tag="PLR47_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG11795.1"
                     /translation="FEGETLGLVGESGCGKSTMARLLIRLEKPDEGSVSFRGRDIISL
                     ASEDVKTYRRRVQMIFQDPYSSLNPRKSVQSIISEPLTIFRAADRTKRKEKVLQLMKK
                     VGLSEEQARRYPHEFSGGQRQRIGIARALALQPELVIADEPVSALDASIQAQILNLLS
                     DLKKDFGLTYLFISHDLNVIHHVSDRIAVMYLGRIVELADKNDLYREPLHPYTRMLLA
                     AMPGGDPQRKKWGTQVRGEAAEARQAGCSFKNRCIYKIAVCDETVPELKPYPGRGLCA
                     CHRTGEI"
     gene            1200..1820
                     /locus_tag="PLR47_02295"
     CDS             1200..1820
                     /locus_tag="PLR47_02295"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG11796.1"
                     /translation="MHHEEKVFWSNGSLTGEGLWHSGAVDAGAVICHPHPLMGGSMHN
                     NVVETVRDVWSAAGFSTLRFNFRGVGKSTGRYDEGRGEKQDLLSACAYAKSRGVKKLF
                     LIGYSFGAWICCRLLRDNPSVATSVLLVSPPLQYFEFDWDGLENTVELMICGDQDTFC
                     DVETLSKKAESISAKLTILSGADHFYFGHEARLAEDLKRYIHEKKA"
     gene            2046..2246
                     /locus_tag="PLR47_02300"
     CDS             2046..2246
                     /locus_tag="PLR47_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOG11797.1"
                     /translation="MAEGKVKWFNEKKGFGFIENDEGGDVFVHYSAIAGTGFKTLYEG
                     QRIRFDIEQGNKGPSAANVQPL"
     gene            2310..3233
                     /gene="miaA"
                     /locus_tag="PLR47_02305"
     CDS             2310..3233
                     /gene="miaA"
                     /locus_tag="PLR47_02305"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOG11798.1"
                     /translation="MKKNLIVILGPTASGKTSLAVRLAAELGGEIISADSRQVYRGMD
                     IGTGKDLSDFHLEGRVIDCHLIDILDPDQEFNVFEFQKRFYEIFSTLRKKQVLPLLVG
                     GTGLYLESVLTRYFMPNAEPDARLREELAGLAMEELQDILLSMKPQLHNQTDLQDRER
                     LIRKIEIEKARQNPSNHPDRFPQIHAAVFGVYWERAELKKRISARLLQRLEEGMIEEV
                     SRLHDEGVSWGRLESFGLEYRFIARCLQKKITREEMAAGLETAIGQFAKRQMTWFRRM
                     EKKGVSIEWIPGGDYSALRGRVLDALQRYSP"
     gene            3230..4585
                     /locus_tag="PLR47_02310"
     CDS             3230..4585
                     /locus_tag="PLR47_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11799.1"
                     /translation="MKKASLINDYLSRYAAGPSWRLRAEDSGSVEQVVVIPAYAERDL
                     IFFTLASLAANPEAMLEKTMVLCVVNNKADSPKEDRENNALTLALLDALAKRRRLDMP
                     DADGNLQEAWQRVASSRLRLGFVDASSPGLEIPRRAGGVGMARKIGMDAALGLLANSK
                     QKLRLILSLDADTLVKPDYLTGVRTAFSSGRAQAGMVDYEHQMPSDRESQEAICRYEI
                     FLRYWVLGLKFAKSPYAFHSIGSTMAATDEGYLAVRGMNRREAGEDFYFLNKLAKAGP
                     LRRIHETRVYPSARLSNRVPFGTGAAVAKLVSASSGDFGLYDPRVFAVLERWLTLMKE
                     SFEGTANEILAQAKEIHSGLGEFLDGRKFLSVRPKIRGNVKDRRAYERQMHGWFDGFE
                     TLKLINYLTLYFYPKVSLPFAVKKLLEMQNPACPSPFSAEGATGLEEGMRMLHALRRL
                     T"
     gene            4690..>5663
                     /locus_tag="PLR47_02315"
     CDS             4690..>5663
                     /locus_tag="PLR47_02315"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG11800.1"
                     /translation="MNKFWFKSVLMIFVLALVVPMLACSKEEGTANTTAPAASQSTAS
                     ESILVPEKPAEEPAAPAPASVQALAADAAVSVDGIVLKKSELDKKVQAQMKLYQDKIP
                     ADRKNEVRSGLKKQLTEDFVMRTLLANEVKRRNITATDKEIQSAMDQIKENLPPDKNL
                     KEFLKENNVSRDDIDLGVRIQKLAAMETGGKIKPSEKEISKFYADNKDKFTTGESVHV
                     RHILVAIDARDDEKIKAEKKAKIEGLRKQVLAGADFAEVAKKNSDCPSKENGGDLGEI
                     KRGQTVKPFEDAAFSQEIKTVGPVVSTEFGHHVLQVLGRTPAKTVGLD"
     assembly_gap    5664..5763
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5764..6106
                     /locus_tag="PLR47_02320"
     CDS             <5764..6106
                     /locus_tag="PLR47_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11801.1"
                     /translation="AIPYRDEYIIAVGSSFEKYLASLDKLAGLDVRMICADHYGCVEG
                     VEAREYIGQTRAAALQMMDRMREALAQEGSVEAAARQLIRLHYERRPDYFVHPDILLR
                     TYTQMLKQLTG"
     gene            complement(6111..6929)
                     /gene="panB"
                     /locus_tag="PLR47_02325"
     CDS             complement(6111..6929)
                     /gene="panB"
                     /locus_tag="PLR47_02325"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOG11802.1"
                     /translation="MSTQTKISRKTILDIKKMKTQGEKITMLTAYDFGMASILDESDI
                     DILLVGDSLGNVVLGYDTTLPVTMEDMIHHTRAVARGSVKALIVADMPFLSYQVSQEA
                     ALANAGRFLKEGNAQAVKLEGGREHAEIVRKMTLSGIPVMAHLGLTPQSVHQLGGYKV
                     QGKKEDAAEKMMKDAVILEEAGAFSVVLECVPEKLAAEITRILSIPTIGIGAGVSCDG
                     QVLVVNDMLGMNDRMTPKFVKRYAHLSQEIKNAVKRYASEVKTGAFPDAEHSFK"
     gene            complement(6913..7812)
                     /locus_tag="PLR47_02330"
     CDS             complement(6913..7812)
                     /locus_tag="PLR47_02330"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOG11803.1"
                     /translation="MKKKSSLTFAVVGTGMVGTAIGFLLQKSGHNVAAVADLSAAHLK
                     RAASYIRAPGFHHPAQAAKLADCILITTPDGRIAAACAQIVSGSDVKGKKVFHMSGAG
                     GLDLLAPAARAGALTASIHPLQSFSSIDGAVSSIPGSYFGVTAAAGAKRLAADIVRDL
                     QGIPIPVSAAQKPLYHVAACIASNYLVSLLSVVEAVYRSIGFSEKDARRAYLPLVYGS
                     LKNIENQGCPNALTGPIARGDSETIQKHIDAMARHLPDFASMYAHLGAIAVKLAREKG
                     TLSAAQAKEILGMLKGAKHEHAD"
     gene            8023..8667
                     /locus_tag="PLR47_02335"
     CDS             8023..8667
                     /locus_tag="PLR47_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOG11804.1"
                     /translation="MKKVELMIFDFDGTLADTGADIAASVNHALRALGLASRPVGEIL
                     SFVGDGVRILMERALGDDHRSRLEEAMEFFLQHYENHLLDHTKLYPAAEEVLNHFRSK
                     KKVILTNKRYYLTVAIAKGLKIDRHFLEIVGADSTPFQKPDGRVISCLLEKYDVAPHR
                     AVMIGDGRNDVEAARNAGTLSCVHLNGLGNREAMLKMGADAYVERLTEIKSLFQ"
     gene            8670..10079
                     /gene="tilS"
                     /locus_tag="PLR47_02340"
     CDS             8670..10079
                     /gene="tilS"
                     /locus_tag="PLR47_02340"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOG11805.1"
                     /translation="MIRKVEQTIRKYSMLEKGDRVVVALSGGADSCALLGALAALAPP
                     WRLELIAAHFNHGLRGEESDGDEAFCRSLAGKTGLVFATQKLAETAIPAGLSPEDYLR
                     RERFRFLDRTAEDYRANKIALGHHRDDQAETFLINMIRGSGLDGLKGFLPVRENRYIR
                     PLIGVSREEILGFLRERGAGFREDASNSEGIYLRNRVRLDLIPFLREKFNPQMEQTLA
                     RTAEIIRRDDSFIDECVRKVLCLPHIRKEENEISFSASSVLSLHDALVYRVLKSLLEG
                     LAAGEGGFSFSHLQALAGLLKESRTGKRLSLPHGIAARREYDRVILTAEPEESSPRYE
                     YPLMIPGEVEVRERRMIFSIRREKEVPVNLKDRSAFFFDGDKIREPLVVRNRREGDRF
                     EPLGTKGSQKIKKLFMDRKIPRRERGRIALLADRESVIWIENMHFSERVKVTPETKNL
                     LVLEVRPKTAGRRPAQENI"
     gene            10138..11952
                     /gene="ftsH"
                     /locus_tag="PLR47_02345"
     CDS             10138..11952
                     /gene="ftsH"
                     /locus_tag="PLR47_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOG11806.1"
                     /translation="MNQAQKNIALVLTVLLVFLLVWQLFNQQNTTTKEITYTDMLTHL
                     ESGEITEVTIQGEAVSGKLANGSVFKTYAPKDDGLVGMFREKNVKITAKPLEENPWYM
                     ILVSWFPMLLLIGVWIFFMRQMQAGGGKAMSFGKSRARLITDKSKKVTFADVAGVNEA
                     KAELEEVIDFLKDPKKYTKLGGRIPKGLLLVGPPGTGKTLLARAIAGEADVPFLSISG
                     SDFVEMFVGVGASRVRDLFSQAKKNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLN
                     QLLVEMDGFESNEGVILVSATNRPDVLDPALLRPGRFDRQVVVPLPDVKGREKIFEVH
                     ARRLPVADDVNYAILARGTPGASGADIENLVNEGALNAARLNKEKVNMADFEFAKDKI
                     LMGTERKSMVISDLEKQHTAYHESGHTLVARLIPGTDPIHKVTIIPRGRALGLTQQLP
                     VDEKHTYPSRYLENNIAVLLGGRAAEELILKDFTTGAGNDIERATNLARKMVCEWGMS
                     KTMGPLSYGKKEEQIFLGREFATHKDYSEETARMIDEEVVSIVKRNYDKAVSLLAEHI
                     DILHRMSRELLEKEVLNASEIDEIIGTALPVEHAAQTS"
     gene            11970..13175
                     /gene="folP"
                     /locus_tag="PLR47_02350"
     CDS             11970..13175
                     /gene="folP"
                     /locus_tag="PLR47_02350"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOG11807.1"
                     /translation="MKIVAIHHDGEALALFQKIGVDPYGIGAMTPKTRHVNILLSARP
                     CKVANILKQEMLSLGADAAVSRGSVSCAVEATDVLLMGTHKQIGALAGKIENQPFGLD
                     VLARDLRVLLGRLEKKPLLLKTVKREIVLGERTLIMGVLNVTPDSFSDGRRYLNFSSA
                     VNRGLQMAEEGADIIDIGGESTRPGAKTVALKEEIARVVPVVEELSAKLTIPLSVDTM
                     KAGVADKALKAGAEIVNDVSALSGDRKMASVVRKASAALVLMHMRGIPADMQEGDLRY
                     ADLTGEIFMFLEKAVQKALAAGISRERLVVDPGIGFGKTHADNCRILNKLCEFRALGL
                     PLLVGTSRKAFIGSVTGDAVDRRMEGTAATVAAAVMNGCHIIRVHDVAAMKKVAAMTD
                     AIVRAGMDS"
     gene            13172..13582
                     /gene="acpS"
                     /locus_tag="PLR47_02355"
     CDS             13172..13582
                     /gene="acpS"
                     /locus_tag="PLR47_02355"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOG11808.1"
                     /translation="MIYGIGIDLVENDRIEKIIRKWGPKFLSRVFTDAEIEYCGRHAQ
                     SSLHYGARFAVKESFLKAIGTGLGGGVKLAEIEVANLKSGKPGLRLSGGARAFVEQAG
                     IQKIHLSITHTKHYASAAVLLEKELPDGGFADKT"
     gene            13579..14058
                     /gene="tsaE"
                     /locus_tag="PLR47_02360"
     CDS             13579..14058
                     /gene="tsaE"
                     /locus_tag="PLR47_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOG11809.1"
                     /translation="MNRAEIELVSENPGQTFEIGLSIGKRAADGDVFALSGELGSGKT
                     CFTGGLARGLGIGEDYAITSPTFTLINEYSGRCRLYHCDVYRLSAAEELADLGFDEYL
                     SGKGVAAIEWAEKIPEALPPDAVFVSFAYVDENRRKIRISGPAQRVRDLLRDISREV"
     gene            14063..>14395
                     /locus_tag="PLR47_02365"
     CDS             14063..>14395
                     /locus_tag="PLR47_02365"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417432.1"
                     /note="catalyzes the formation of 4-phospho-L-aspartate
                     from L-aspartate and ATP, in Bacillus, lysine sensitive;
                     regulated by response to starvation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOG11810.1"
                     /translation="MGLVVQKFGGTSVANVEKIKNVAGKAIREKQAGHDVIVVLSAMA
                     GETNRLIDLAQSAAENPDEREYDSLISTGEQVTISLLSITLNSMGYPARSFLGFQVKI
                     LTDQAHQKA"
BASE COUNT         3326 a         3677 c         4303 g         2989 t
ORIGIN      
        1 ttcgagggag aaacgctggg gctcgtcggc gagtcggggt gcggcaaatc cacgatggcg
       61 cgccttctga tcaggctgga gaagccggac gaggggagtg tttctttcag aggccgggat
      121 attatttcct tggcatcgga ggatgtaaag acgtaccggc ggcgcgtaca gatgattttt
      181 caggatcctt attcttcgtt gaacccgcgg aaatccgtcc aaagcatcat ttccgagccg
      241 ctcacaattt tccgggcggc cgatcgaact aagcggaaag aaaaagtgct tcaactgatg
      301 aagaaggttg gcctcagcga agaacaggcc cgacgctatc cccacgagtt cagcggagga
      361 cagaggcagc gcatcggaat cgccagggcg ctggcgctgc agccggagct ggtcatcgcg
      421 gacgagccgg tttccgcgct cgatgcttcc atccaggcgc agattctgaa tttgcttagc
      481 gatctgaaaa aagatttcgg gctgacctat ctttttatct cccatgacct caatgtcatt
      541 catcatgtat cggacaggat tgccgtgatg tacctgggcc gcatcgttga gctggcggac
      601 aagaatgatc tctaccggga gccgcttcac ccctacaccc gaatgctgct ggcggccatg
      661 ccgggcggtg atccgcaaag gaaaaaatgg ggaacgcagg ttcgcggaga ggcggccgaa
      721 gcccggcagg cgggttgcag ttttaaaaac cgctgcattt ataaaattgc cgtttgcgat
      781 gagacggttc ccgaactgaa gccctatccg ggaaggggcc tgtgcgcctg tcaccgcacg
      841 ggcgagatat gatcccaaac cgtttgtgat tttatagaag agacaatgcg ctgtcattgc
      901 gagcaattta ccaggagcta cgcaccaggt tataaatgga agaaactgta atctcccctc
      961 cctcgatggg aggggagggg gagggtgaaa acaaaggctt ccccctcccc tcaatccccg
     1021 cccaccaggg gcggggaagt atcttaggag cagactcttg gtctgcgtgg caatctaaaa
     1081 aaggagattg cttcactttg ctcgcaatga ccagttcttg ttatcgtgag aatacaaaac
     1141 ttaacctgcg tgccacctgg ttatggcgta atcttaaata ttcaaatgga gcaatgacca
     1201 tgcatcatga agaaaaagtt ttctggtcca acggcagtct caccggcgaa ggtttgtggc
     1261 attcgggcgc agtggatgcg ggcgcggtga tctgccatcc gcatccgctg atgggcgggt
     1321 ccatgcacaa taatgtcgtg gaaaccgtcc gcgacgtatg gagcgccgcc ggcttttcca
     1381 cgctgcgctt taacttccgc ggcgtcggga aaagcaccgg ccgctatgac gagggcaggg
     1441 gagaaaagca ggatcttttg tccgcctgcg catacgcaaa aagccggggc gttaaaaaat
     1501 tatttctgat cggttattcg ttcggcgcgt ggatctgctg tcgcctgctc agggacaatc
     1561 cctccgtcgc gacgtccgtt ttgctggtat cccctccatt gcaatacttc gagttcgact
     1621 gggacggact ggaaaacacc gtggaattga tgatctgcgg ggatcaggac acgttctgcg
     1681 atgtcgagac cctctcaaaa aaagcagaat ccatttcggc aaaattgacg atcctttccg
     1741 gagccgatca tttttatttc ggacacgaag cccggctggc tgaggatttg aagcggtata
     1801 ttcatgaaaa aaaagcttga ttttaaataa attggtgtta tgttctgatc aaatgcatcg
     1861 gttttagagc cggttttaca agggttggaa gcttcgggtc ggtaaataag tagtgttgag
     1921 attgatttac aaaaaaagaa aataaccgca aagagttctt tttgaaataa gaattcttgc
     1981 ggttttttta gtttctgacg ggatgaaccg gaagaaacaa aaatttgaaa gggggggctt
     2041 tagaagtggc agaaggtaaa gtgaagtggt ttaatgagaa gaagggtttt ggcttcattg
     2101 aaaatgatga aggcggagat gtttttgtac attatagcgc cattgcggga acgggtttca
     2161 agactctgta cgagggtcag agaattcgtt ttgacattga gcagggcaac aaagggccca
     2221 gcgcggcgaa tgttcagcca ctctagtcgc tgaaaacgat gaacaaaaaa atgccccgtc
     2281 ttgaggggca ttttttttag ctgaaaatca tgaaaaaaaa tctgatcgtc atcctgggcc
     2341 cgacggcttc gggcaaaacg tcgctggccg tccgtctggc cgccgaactc gggggagaaa
     2401 tcatttccgc ggattcgcgc caggtttatc gcgggatgga tatcggcaca ggcaaggatc
     2461 tgagcgactt tcatttggag ggccgcgtca ttgactgcca tctgatcgac attttggacc
     2521 cggatcagga gttcaatgtt ttcgagtttc agaaacgctt ttacgaaata ttttccacgc
     2581 tcaggaaaaa acaagtcctg ccccttctgg tcggcggcac aggcctgtac ctggaatcgg
     2641 tcctgacccg atacttcatg ccgaatgccg aacccgacgc gcgcctgcgg gaagagctgg
     2701 cggggctggc gatggaagag cttcaggata ttttgttgtc catgaaaccc caactgcaca
     2761 atcaaaccga tcttcaagac cgggaacggc tgatccggaa aatagaaatc gaaaaagcca
     2821 ggcaaaaccc gtcaaaccac ccggaccggt tcccgcaaat ccatgccgct gtcttcggtg
     2881 tttattggga gcgcgccgaa ctgaaaaaaa gaatctcggc gaggcttctc cagcggctgg
     2941 aggaggggat gattgaagaa gtgtcccggc tgcacgacga gggagtgtcc tggggaaggc
     3001 tggagagctt tggtctggaa taccggttca tcgcccggtg cctgcaaaag aaaataacca
     3061 gggaagagat ggcggccggc ctagaaaccg ccatcgggca gtttgccaag cgccagatga
     3121 catggtttcg ccgcatggaa aaaaagggcg tttcgattga gtggattccc ggcggcgatt
     3181 attccgcgct gcgcggccgc gtcctggacg cgcttcagag atattctccg tgaagaaagc
     3241 ttccctgatc aatgattatc tgagccggta cgcggcgggg ccgtcatggc gtctgcgggc
     3301 ggaggattcc gggtccgtcg aacaggttgt cgtcattccg gcttatgcgg aaagagactt
     3361 gatctttttc acgctggctt ctctcgccgc caatcccgag gcgatgctgg aaaaaacaat
     3421 ggtgctgtgc gtggtcaaca acaaagccga ctcgcccaaa gaagacaggg aaaacaacgc
     3481 tctaaccctt gcgcttctcg atgcgctggc gaaaaggcgg cgtttggata tgccggatgc
     3541 ggacggaaat cttcaggagg catggcaaag agtcgcctcc agccgtttgc ggctgggatt
     3601 tgtggatgct tcttctcccg gactggaaat tccccgccgc gcgggcggcg tcggaatggc
     3661 gcgcaagatc ggcatggacg cagctctcgg cctgctggca aattcgaaac aaaagctccg
     3721 tttgattctg agccttgacg cggatacgct ggtcaagccg gattatctga caggcgtccg
     3781 cacagctttt tcctccggcc gggcgcaggc cggcatggtg gattacgaac atcaaatgcc
     3841 ttcggacagg gagagtcagg aggcgatttg ccgctatgag atcttccttc gctactgggt
     3901 tctgggtctc aaatttgcaa aatcacccta cgcgtttcat tccatcgggt ccacgatggc
     3961 ggcaacggat gaaggatacc tcgccgtgcg gggcatgaat cgccgcgagg cgggcgagga
     4021 tttttatttt ctgaacaagc tggccaaggc gggccccctg cgcagaattc acgaaacgcg
     4081 agtttatcct tccgcgcggc tttcaaatcg cgttcccttc gggacgggcg cggcggtggc
     4141 caaactggtt tccgcctcgt ccggtgactt cgggctgtat gatccgcgcg tctttgccgt
     4201 tcttgaaagg tggctcacac tgatgaaaga atcttttgaa ggcacggcga atgagattct
     4261 cgcacaagcg aaagagattc attcgggcct tggggaattt ttggacggac ggaaattttt
     4321 gtcggtccgg ccgaaaattc gcggcaacgt gaaggatcgc agggcctatg aaagacagat
     4381 gcacggctgg tttgacggct tcgaaacatt gaagctgatc aattatctga ctctgtattt
     4441 ttatccgaaa gtttccctgc ctttcgcggt gaagaagctt ctggagatgc aaaacccggc
     4501 atgcccgtcc ccgttctccg ccgaaggagc gaccggcctt gaagagggca tgaggatgct
     4561 ccatgccctt cggcggctga cctgaaagcg ggaattcgac aacgccgggg ataatcttct
     4621 tgactttgca aaagaacttc gcgtatagcg caaaaacatg acgcctgcag agggtccggg
     4681 aggaaattca tgaataaatt ctggttcaaa agcgttctga tgattttcgt gctggcactg
     4741 gttgtaccga tgcttgcctg cagcaaagag gaggggacgg caaacacaac cgcaccggct
     4801 gcttcgcaaa gcaccgcttc cgaatcgatc ctggtgccgg aaaaacccgc cgaagagccg
     4861 gcggcgcctg cacccgcttc cgttcaggcg cttgccgccg acgcggccgt cagtgttgac
     4921 ggcattgtcc tgaagaagag cgagcttgac aaaaaagttc aggcccagat gaaactgtat
     4981 caggacaaga taccggccga ccggaaaaac gaagtgcggt ccggtttgaa aaaacagttg
     5041 acggaagatt ttgtgatgcg gacgcttttg gccaatgaag tcaaacgcag aaacatcacg
     5101 gccacggaca aggaaatcca gtcggccatg gatcagatca aagaaaatct tccacccgat
     5161 aaaaacctta aggaatttct taaggaaaat aacgtatccc gcgatgacat tgatctgggt
     5221 gttcgcatac aaaaactggc ggccatggaa accggcggaa aaatcaagcc gtcggaaaag
     5281 gaaatcagca agttttacgc ggacaacaaa gataagttca ccaccgggga aagcgttcac
     5341 gttcggcata tcctggtggc catagacgcc agggacgacg agaagatcaa ggctgaaaag
     5401 aaggcaaaga tagaaggcct gcgcaagcag gtcctggccg gcgccgattt cgccgaagtg
     5461 gccaaaaaaa attccgactg ccccagcaag gaaaacggcg gggatctggg cgaaatcaag
     5521 agggggcaga cggtgaagcc ctttgaggac gcggcgtttt cgcaggagat caaaacggtc
     5581 ggaccggtgg tgagcacgga attcggccat cacgttcttc aggtgctggg gcgcacaccg
     5641 gccaaaaccg tcggtctgga cgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnggccatt ccctaccgcg acgaatacat catcgccgtc ggttcgagct ttgaaaaata
     5821 cctggccagc ctggataagc ttgccggcct ggacgtccga atgatttgcg ctgaccatta
     5881 cggatgcgtt gagggagttg aggcgaggga atacatcggg caaaccagag ccgcggcgct
     5941 tcagatgatg gaccggatgc gcgaggcgct ggcgcaggag ggaagcgtcg aggccgcggc
     6001 ccggcaattg atcaggcttc actacgaacg aaggccggat tactttgtcc atccggacat
     6061 tctgcttaga acctacacgc aaatgctcaa acaactcacc ggataaaaaa ctatttgaag
     6121 ctgtgctccg cgtccggaaa agcgccggtt ttcacctctg aggcgtagcg cttgacggcg
     6181 tttttgattt cctggctcag atgggcgtat cttttgacga atttgggcgt cattcggtcg
     6241 ttcatgccca gcatgtcgtt tacgaccagg acctgcccgt cgcaggaaac ccccgcgccg
     6301 atgccgatgg tcggaatgga caggatgcgg gtgatttccg cggccagctt ttccggcacg
     6361 cattccagca ccacggaaaa ggcgcccgct tcttcgagaa tcacggcgtc cttcatcatt
     6421 ttttccgccg cgtcctcctt cttgccctgt actttgtagc cgccgagctg gtggaccgat
     6481 tgcggcgtaa gccccagatg cgccatgacc ggaatgccgg agagcgtcat tttacggacg
     6541 atttccgcgt gctcccggcc cccttcgagt ttgacggcct gagcgttgcc ctcttttaaa
     6601 aaacgtccgg cattggccag cgccgcctcc tgcgacacct ggtaggaaag aaaaggcatg
     6661 tccgccacaa tcagcgcctt gaccgatccc cgggccaccg ctcttgtgtg atggatcatg
     6721 tcctccatcg tcacgggaag cgtggtgtcg tagcccagaa cgacattgcc cagagaatcg
     6781 ccgacgagca gaatgtcgat gtcgctttcg tccagaatgg acgccatgcc gaagtcgtag
     6841 gccgtcagca tggtgatttt ctcgccctgc gttttcattt tcttgatatc cagaatcgtt
     6901 ttccggctga tcttagtctg cgtgctcatg ttttgctcct ttcagcattc ccagaatttc
     6961 cttggcctga gcggcgctca gtgtgccttt ttccctggcg agtttcaccg cgatggcgcc
     7021 caagtgagca tacatcgaag cgaagtcggg aagatggcgt gccatggcgt cgatgtgttt
     7081 ctgaatggtt tccgaatcgc cgcgcgcgat cggtccggtg agggcgttcg ggcagccctg
     7141 attctcaata tttttcaaac tgccgtacac cagcggcaga taggccctgc gggcgtcttt
     7201 ctccgaaaaa ccgatggacc tgtaaacggc ttcgacgacg ctcaaaagcg agacgaggta
     7261 attcgatgcg atgcaggcgg ccacgtggta cagaggcttc tgcgcggcgg agacgggaat
     7321 gggaatgccc tgaagatcgc ggacgatgtc cgccgccagt ctctttgcgc ccgcggcggc
     7381 cgtgacgccg aagtagcttc ccggtatgct ggaaacggct ccgtcaatgg aggaaaaact
     7441 ctgcagcgga tgaatgctgg cggtgagagc gccggcccgg gcggcggggg cgagcagatc
     7501 caggcctccg gcgccgctca tgtgaaaaac ttttttcccc ttcacgtccg agccggaaac
     7561 gatctgggcg caggcggccg cgatgcggcc atccggcgtc gtgatcagaa tgcagtcggc
     7621 gagcttagcc gcctgggccg gatgatgaaa ccccggcgcc cgaatgtagg acgcggcgcg
     7681 tttcagatga gcggcggaaa gatcggcaac ggccgcgacg ttatggcctg acttctgcag
     7741 cagaaatccg atggctgtgc cgaccatgcc ggtcccgacc acggcgaagg tgagcgaaga
     7801 cttttttttc attttcacgt ccataaaaaa agggtcttcc gcggcgtaag gaagacccgt
     7861 atttgacagt tttgtggcga ctcccgtctc agtctgcaac aagtgatcca agcggttgga
     7921 agcttcctag cacacggggc ctgagccgtc aaataatttt tccccgccaa aatagcttgt
     7981 ttttgcttga ttttgcggct aaacagccaa caggggaaca aaatgaaaaa agtggagttg
     8041 atgatattcg attttgacgg aacgctcgcg gacacgggag cggacattgc cgcttccgtc
     8101 aatcacgccc tgagagcgct ggggctggca tcaaggccgg ttggggagat tctcagtttt
     8161 gtcggagacg gcgtgcggat tttgatggaa agagcgctgg gtgatgatca tcgctcaagg
     8221 ctggaggagg caatggaatt tttccttcaa cattacgaaa atcatctgct ggatcacacg
     8281 aagctctacc cggcggccga agaggtgttg aatcactttc gttccaaaaa gaaagtcatc
     8341 ctgaccaaca agcgttatta cctgacggtg gcgatcgcca aagggctcaa gatcgaccgg
     8401 cattttctgg aaattgtagg cgcggattcg acgccgtttc aaaagccgga tgggcgagtc
     8461 atttcctgtc ttctggaaaa gtatgatgtc gccccgcaca gggccgtgat gatcggcgac
     8521 ggccgcaacg atgtggaggc ggcccgaaac gccggaacct tgtcctgcgt tcatttgaac
     8581 ggactgggca accgggaggc aatgctcaaa atgggcgccg atgcttatgt ggagcgcctg
     8641 acggaaatca aatccctgtt tcaatgacga tgatccggaa agtagaacag accattcgaa
     8701 aatactccat gctggagaag ggcgaccggg tggtggtcgc tctgtccggg ggggcggatt
     8761 cctgcgcgct gcttggggcg cttgcggctc ttgcgccgcc gtggcgtctg gaattgattg
     8821 ccgcccattt caatcacgga ctgcgcggcg aagagtctga cggggacgaa gcgttttgcc
     8881 gttcgctggc cggaaagacc ggtctggttt ttgcaacgca aaagctggcc gaaaccgcga
     8941 ttcccgcggg cctgtccccg gaagactatc tgcggcggga aaggtttcgc ttcctggacc
     9001 ggacggccga agattacagg gcgaataaaa tcgcgctcgg acatcaccgc gacgaccagg
     9061 cggagacctt tttgatcaac atgatccgcg gaagcggtct ggacggactg aaaggctttc
     9121 tgcccgttcg ggagaaccgg tacatccgac cgctcatcgg cgtatcccgg gaggaaattc
     9181 taggttttct cagggagcgg ggcgcgggct ttcgcgagga cgcatccaat tcggaaggga
     9241 tttatctgcg caatcgggtc cgcctggatc tgatcccctt tcttcgggaa aaattcaatc
     9301 ctcaaatgga gcagacgctg gcgaggacgg cggaaattat ccgccgcgac gactcgttca
     9361 tcgatgaatg cgttcggaag gttctgtgcc tgccgcatat tcgaaaagag gaaaacgaaa
     9421 tttccttttc cgcttcgtcc gttctttccc ttcatgacgc gctcgtttac cgggttctta
     9481 agtctcttct ggagggcctg gcggccgggg aaggcggttt ctccttctcc catcttcagg
     9541 cgctggccgg cctgctgaaa gagagccgga cgggaaaaag gctctctctg ccgcatggaa
     9601 tcgccgcccg cagagaatat gaccgcgtga tcctcacggc tgaacccgaa gaaagcagtc
     9661 cgcgctacga atatcccctg atgatccccg gcgaagtcga agtccgggaa agacggatga
     9721 tcttttccat ccggcgggaa aaggaagtcc ccgtgaattt aaaagaccgc tccgcgtttt
     9781 tctttgacgg agacaaaatc cgggagccgc tggtggtccg aaaccgccgg gaaggcgacc
     9841 ggtttgagcc gctgggaaca aaagggtccc agaaaatcaa aaagctgttc atggaccgca
     9901 agattcccag acgggagcgc ggccgcatcg cgcttctggc cgaccgggaa tccgtgatct
     9961 ggattgaaaa tatgcatttc agcgagaggg tgaaggtgac gcccgaaacg aaaaacctgc
    10021 tggttttgga agtccggccc aaaaccgcgg gccgccgccc cgcacaagaa aatatttgag
    10081 ttaaaccgga tattattata aaggttgccc aacagtttaa tttcaaggag aaagatattg
    10141 aatcaggctc aaaaaaacat agcgcttgtc ctgacagtgc ttctggtatt cctcctggtc
    10201 tggcagttgt tcaatcagca aaacaccacc acgaaagaaa tcacctacac ggatatgctg
    10261 acgcatttgg aaagcggcga aatcacggaa gttaccattc agggagaggc cgtttccggc
    10321 aagctggcca acggcagtgt ttttaaaacc tatgccccga aagacgacgg tctggtcgga
    10381 atgtttcgtg agaaaaatgt caaaatcacc gccaaaccgc tggaagagaa tccctggtac
    10441 atgattcttg tgtcctggtt tccgatgctg ctcttgatcg gcgtgtggat tttcttcatg
    10501 cgccagatgc aggcgggagg cggcaaggcg atgtctttcg gaaaaagccg ggcgcggctg
    10561 atcaccgaca agtccaagaa ggtgaccttt gccgatgtgg ccggcgtgaa tgaagccaag
    10621 gcggagctgg aagaggtaat cgacttttta aaagacccca agaaatacac caaactgggc
    10681 ggccgcatcc ccaaggggct tttgctggtg gggcctcccg gaacggggaa aacccttctg
    10741 gcgcgggcga ttgccgggga agccgacgtg ccttttttga gcatcagcgg ctccgatttc
    10801 gtggaaatgt ttgtcggcgt cggcgcgtcg cgcgtgcggg atttgttcag ccaggccaag
    10861 aagaacgctc cctgcattat ctttatcgat gaaatcgacg cggtcggacg tcaccgcggc
    10921 gcgggactgg gcggcggcca tgatgagcgg gagcagacgc tgaatcagct tctggtcgaa
    10981 atggacggtt ttgagtccaa tgagggcgtg attctggttt cggcgaccaa ccgtcccgac
    11041 gtgctggatc cggcactttt gcgtccgggg cgttttgacc gccaggtggt ggtgccgctg
    11101 ccggatgtga aggggcggga gaaaatcttt gaagtgcacg cacgcaggct tccggtcgcg
    11161 gacgacgtga attacgcgat tctggccagg gggacgcccg gcgcatccgg ggccgacatc
    11221 gaaaatctgg tcaatgaagg cgcgctcaac gccgcgagac tgaacaagga aaaagtcaat
    11281 atggcggact ttgaattcgc caaggacaag atcctgatgg gcacggaacg aaaaagcatg
    11341 gtcatcagcg acctggaaaa gcagcacacg gcctatcacg aatccggcca tacgctggtg
    11401 gcccggctga ttccgggaac cgacccgatc cacaaggtca cgatcattcc ccgcggccgc
    11461 gccctgggat tgacgcagca gcttccggtg gacgaaaagc acacctaccc aagccggtac
    11521 ctggaaaaca atatcgccgt cctgctgggc gggcgcgcag ccgaagaact gattctgaag
    11581 gactttacga ccggtgcggg caacgacatc gagcgggcga ccaacctggc ccggaagatg
    11641 gtctgtgaat ggggcatgag caaaaccatg gggccgctca gctacggcaa gaaggaagag
    11701 cagatctttc tgggaagaga atttgcgacg cacaaggatt acagcgaaga aacggccaga
    11761 atgatcgacg aggaagttgt ctcgatcgtc aagcgcaact acgacaaagc cgtgtccctg
    11821 cttgccgagc acatcgatat tttgcacagg atgtccaggg agcttctgga aaaagaggtc
    11881 ctcaacgcct cggagatcga cgagattatc ggcaccgccc tgcctgtcga gcatgccgcg
    11941 caaacttcat aaacggttga tggcgaattt tgaagatcgt tgcaatccat catgacggcg
    12001 aagcgctcgc cctgtttcag aaaatcggcg tggaccctta cgggatcggc gcgatgacgc
    12061 ctaaaacgcg gcacgtgaac atcctgttgt ccgcccggcc ctgcaaggtc gccaacattc
    12121 tcaagcagga aatgctttct ctgggcgcgg acgcggcggt ttcgcgcgga agcgtttcct
    12181 gcgcggtgga agccaccgat gttctgctca tggggaccca caagcagatc ggcgccctgg
    12241 ccggaaaaat cgaaaatcag cccttcggac tggacgttct tgcccgggac cttcgcgtgc
    12301 ttttgggccg tctggaaaag aagcccttgc tcctgaaaac ggtgaagcgc gaaattgtgc
    12361 tgggagaaag aacgctgatc atgggcgttt taaacgtgac gcccgattcc ttttcggacg
    12421 gcagacgtta tttgaatttc tccagcgccg tcaaccgggg cctgcaaatg gcggaggaag
    12481 gggcggacat catcgacatc ggcggtgaat ccacaaggcc cggagcaaaa acggtcgccc
    12541 tgaaggaaga aatcgcgaga gtggttcccg tggtggagga gttgtccgcg aagctgacga
    12601 tccctctgtc cgtggatacc atgaaagccg gggtcgctga taaagccctg aaggcgggag
    12661 cggaaatcgt aaacgacgtg agcgcgcttt ccggcgatcg gaaaatggct tcggtcgtcc
    12721 ggaaagcctc cgcggcgctc gttttgatgc acatgcgggg aatcccggcc gacatgcagg
    12781 aaggcgatct gcgctatgcc gatctgacgg gcgagatttt catgtttctg gaaaaggccg
    12841 ttcaaaaagc cctggcggcg ggcatcagcc gggagcggct ggtggtcgat ccgggaatcg
    12901 gcttcggcaa aacccacgcg gacaactgcc gaatcctcaa caagctttgc gaattcaggg
    12961 cgctggggct tccgctgctg gtcgggacct cgcgcaaagc gtttatcggc agcgtgacgg
    13021 gggatgccgt ggaccggaga atggaaggca cggcggcgac ggtcgccgcg gccgtgatga
    13081 acggctgtca tataattcgc gtccacgatg tcgcggccat gaaaaaagtg gcggccatga
    13141 cggatgcaat cgtccgcgcc gggatggact catgatttac ggaatcggca tcgatctggt
    13201 ggaaaacgac cgcatcgaaa aaatcattcg gaaatggggc ccgaaatttt tgtcccgggt
    13261 gtttaccgac gcggaaatag aatactgcgg gcggcacgcg cagtcttccc ttcattacgg
    13321 agcgcggttt gccgtcaagg aatcgtttct caaggccatc ggcacgggcc tcggcggcgg
    13381 cgtaaagctg gcggaaatcg aggtggcaaa cctgaaaagc ggaaaacccg ggcttcgcct
    13441 gtccggcggc gctcgggcgt ttgtggagca ggccgggatt caaaaaatcc acctcagcat
    13501 cacccacacg aagcattacg cgtccgcagc ggtcttgctg gaaaaggaat tgccggatgg
    13561 cggatttgcg gataaaacat gaacagggca gaaattgagc tggtgtcgga aaatcccggg
    13621 caaacctttg agattggcct gtccatcggg aaacgggcgg cggacggcga tgttttcgct
    13681 ctgtccggag aactgggctc cggcaagacc tgttttacgg gcgggctggc gagggggctt
    13741 gggatcggcg aggactacgc gatcacgagt ccgaccttta cgctgatcaa cgagtattcc
    13801 gggaggtgca ggctttatca ctgcgacgtt taccggcttt ccgcggcgga agagcttgcg
    13861 gatctggggt ttgacgagta cctgtcggga aaaggcgttg cggccattga atgggcggag
    13921 aagattccgg aggcactgcc gccggatgcc gtttttgtgt cttttgccta tgtggatgaa
    13981 aacaggcgga aaataagaat cagcgggccc gcgcagaggg tccgggattt attgcgggat
    14041 atttcgaggg aggtttaaaa ttatgggtct ggttgttcag aaattcggcg gaacatccgt
    14101 ggcgaatgtt gaaaaaatca aaaatgtggc gggcaaggcg attcgcgaaa agcaggcggg
    14161 gcacgatgtg attgtcgttc tctcggccat ggcgggagaa acgaaccggc tcatcgacct
    14221 ggcccagagc gcggcggaaa atccggacga aagagagtat gattcgttga tttccaccgg
    14281 ggagcaggtg acgatttcgc tgctttccat cacgctcaat tccatggggt atcccgcgcg
    14341 ttcttttctc ggttttcagg tcaaaatcct gacggatcag gcgcatcaga aggcg
//
LOCUS       DAOSIM010000027        14204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_4375_length_14204_cov_5.413669, whole genome shotgun sequence.
ACCESSION   DAOSIM010000027 DAOSIM010000000
VERSION     DAOSIM010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 14204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14204
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4375_length_14204_cov_5.413669"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PLR47_02370"
     CDS             <1..475
                     /locus_tag="PLR47_02370"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOG11811.1"
                     /translation="NRLVHGQILEAWVPFVEARCIMVVDDNSASDFFCETVIRMAVPS
                     DIEVNICSVDDFAANYAYVRGSGKKTIVLFGRIADALKAYEQGFHFEALNIGNIYHED
                     YKVCCAPSVFLCEPEIADIIRLQREKGVAVEVKRVPREKAVDIQDALKASCDSKP"
     gene            488..1183
                     /locus_tag="PLR47_02375"
     CDS             488..1183
                     /locus_tag="PLR47_02375"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HOG11812.1"
                     /translation="MLTQIVILSLVGGLLCLDRIFLQAMLSRPIIIAPVMGLLLGDAY
                     TGLIIGAIFELFWMDRAPIGIYIPPNDSIASAFAAALAILAGQSLGAVTKELTALSVL
                     LAVPFGILAKKMDVRMMAANDLLSDRALEAARSRDLRAIENMTCLALGRAFVLYAGLL
                     FILQVIFIPLVINLYPKLPVQAGGALTLTYYFLPVLGTAVALSTIKLRGAVPVFCAIF
                     LAAVLAMDYFHVF"
     gene            1173..1667
                     /gene="rimI"
                     /locus_tag="PLR47_02380"
     CDS             1173..1667
                     /gene="rimI"
                     /locus_tag="PLR47_02380"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOG11813.1"
                     /translation="MSSDKTGQLFSVDLMREEDLPEILAIEAASFVTPWTRGMFQDEL
                     RLPHAQCLVIRARQAEKTQVAAYIIFWLVADEVHLHNIAVRGEFRRKGLASGLMNLMR
                     DIAGQAGADRQTLEVRESNTGAINLYGRCGFVVKGKRPRYYDDTREDALVMWAEVRQN
                     GHHE"
     gene            1660..2475
                     /locus_tag="PLR47_02385"
     CDS             1660..2475
                     /locus_tag="PLR47_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOG11814.1"
                     /translation="MNKQITIGSVSGNDEIRPGYVLMTIHVHRSFPEPRPGQFVMIRI
                     AGLQEPFLSRPLSIFSFSRGRSHGLVKLLYRVAGKGTQILAGLIRGSQVEIVGPLGKG
                     YTVDPSRENRVLIAGGIGVAPLSLLAQHLCRLSGRGANPAVVYLGAKTADELVGLDQL
                     HKYCNSIRVCTDDGSLGEKSLVTRTFQKDMKKHDPARTSIYACGPKPMLQSLAGILKD
                     RYFCQVSLEERMACGVGACMGCAVAVRDENGDKTYRRVCADGPVFDLQKVIWE"
     gene            2572..3474
                     /locus_tag="PLR47_02390"
     CDS             2572..3474
                     /locus_tag="PLR47_02390"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOG11815.1"
                     /translation="MVVEIAGLKLKNPVMTASGTFGCGEEYAGYVDLNRLGGIVVKGL
                     SLKPRLGNPPPRIMETAGGMLNAVGLQNIGVDAFIEEKLPFLRTYDVAVIANIYGESC
                     AEYAQVARKLSAAPGVHALEVNVSCPNVKKGGLSFGADPKAAAEVTKRVKAETHLPVI
                     VKLTPNVTDITVIARAVEEAGADAVSLINTLTGMSIDLKTRTPHLKNITGGLSGPAIK
                     PVALRMVWQAAQKVTIPVIGMGGIMTAEDALEFMVAGAAAVQVGTANFINPLATSEIV
                     QGMRQYVKHHKLKNITEIVGTLKV"
     gene            3716..4402
                     /locus_tag="PLR47_02395"
     CDS             3716..4402
                     /locus_tag="PLR47_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG11816.1"
                     /translation="MRRGTEEILSGVYLIGGPNVTQADDAAVYLIDFKGELVLIDAGA
                     GGSASQLVRNIEMLGLNPALVSHVILTHCHIDHIGAAPFFREKYKAKIVIHEMDAKAL
                     ETGDSVRTAANWYGTTFPPTKIDRKLTGARETLKFDNDELHCLHTPGHTPGSIAVYLD
                     RSGQRVLFGQDIHGPFHKAFGSNIEAWKKSMQTLLALEADILCEGHFGIFRPKNKARA
                     YIERYLEEYE"
     gene            4450..5184
                     /locus_tag="PLR47_02400"
     CDS             4450..5184
                     /locus_tag="PLR47_02400"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HOG11817.1"
                     /translation="MRKTLFVFFMLFWAAGITGAAQAGIFDDILKKLPAAKSAAPSAG
                     LDQKTTISGLKEALVISSKNAVKAVSKKDGYFGNQVVKILLPDNIQKAADVVAKLGYQ
                     KQVDHLILSMNRAAEAAAPKAIPLFADAIKSMSIDDARKILQGGDAAATEYFKEKTSA
                     KLYEEFKPVVVANMNKVGVAKAYKNMTAPYEALPLVSSKSIDLDRYVTSKALDGLFLI
                     IAEEEKKIRTDPAARVTDLLKTVFGK"
     gene            complement(5370..6179)
                     /locus_tag="PLR47_02405"
     CDS             complement(5370..6179)
                     /locus_tag="PLR47_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG11818.1"
                     /translation="MLILKGKNVLITGAASGIGKALAECFAREGSNLFLGSHPREEDQ
                     LCRWADYLEHQYHIKTKKFPVDLSLASGPELLYRQVEQTGKTIDILVNNAGIMLYGDF
                     ANLPLDGQLEVITVNAIAYLKLMRLFLPGLIEKKSGRILNVVSASAFQPTPHHAVYGA
                     TKAFVQSLSEAVGQEIKSSGIKICTLNPSYTDTPMLRGKGFPKKLWWYSISGLSSPEN
                     IARKGVKAFKKGKPIYIPGIQNWFAASVLTRVFPRRLASYVPSLVLREKGN"
     gene            complement(6320..7558)
                     /locus_tag="PLR47_02410"
     CDS             complement(6320..7558)
                     /locus_tag="PLR47_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOG11819.1"
                     /translation="MGNVYIIGVGMIRFNKYPDKTVISMAHEATDLALKDAGLAREDL
                     DAVFFSNTFWGMFDNQHSIRGEVVMRRMGVGKVPVVNVENACAGGSTALHLAYTGIRS
                     GMYDVAMAIGSEKITNPNKMLSLSAYGTCMDVGNFEQHIQNLMEISKTFTVKIPAGQS
                     APGEGRSIFMDAYAMGARWHMSRFNSTQQQLAVICAKNHLHGSLNPLSQYQQVMTAEE
                     VLADKMVAYPLTRAMCAPVGDGSAATIICSEKFLKKMTSARPVQIRASVLGTGADRDL
                     NAPDIGERLAKQAYHMAGVGPEDIDLAELHDATSWGELHQTESMGFCPMGEGGPFAES
                     GATTLGGKKPINTSGGLECKGHPIGASGLSQIHELVMQLRGEAGKRQVEGAKIGLAEN
                     GGGNIGVEEAAMCIHILEKM"
     gene            complement(7551..8789)
                     /locus_tag="PLR47_02415"
     CDS             complement(7551..8789)
                     /locus_tag="PLR47_02415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG11820.1"
                     /translation="MAYRDLNLDLTDEHIALKESVHKFSMEVLRPASIELDKLSPEEV
                     IAKDSLYWKCMRQMYKNKYHTALISDAYGGMGLDPLSVHIFFEELAYGSAGFTVSLGV
                     SIFSAFYACMVPDDHLVNKFIIPFVECEDASIMSCWSITEPEHGSDILMPGTPFFHDP
                     KITQQVKAKNGDWIINGQKAAWVSNGPIASNAALFVNIDSAQGMSGGGICLIDLTQPG
                     VTRGKPLDKMGQRELPQGEIFFDDAICPGEDMIIDPESYEMMTDITLATANACMGAFF
                     TGVAQAAYDLAVQYCKERVQGGKTISNHQWVQKKIFDMFSKVQAARQLSRAAMIYNMN
                     TTPPAVPYSVAAKVFCTNAAFEVASDAVQLFGGNGVSKEYPIEKLFRDARASMIEDGS
                     NDSLAIGIGTALLKEEYANG"
     gene            complement(8864..10477)
                     /locus_tag="PLR47_02420"
     CDS             complement(8864..10477)
                     /locus_tag="PLR47_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG11821.1"
                     /translation="MASKAHLNQVIASHLIEIRADEMPDKEIYIFENGEHGEDILTYR
                     ILHERSNKIARLFMDKGIGKGDTFAIYMRNHPEFVCGMMAGITLGAIAVPVDPRNKRQ
                     RLKFLLNNCKARAVIVSGECLEDLEAVITDVPTLRFITVAYQPGQNAAVSAKHHALNE
                     VMEKDTWKPVEQQIMDVRHPMQIIYTSGTTGDPKGVMIRNNRTGLFNILNSIVWKYKS
                     DDILYNGLSLTHGNAQAVTLFPALSLGVKAVFSPRFTKSRIWDICRKYGCTSFSLLGG
                     MAAGIYNEPEKHDDGDNPVKVVISAGTPMAIWEGFEKRFNVQILEWYGAVEGGFAYKP
                     TGQGPVGSFGKPVPGVMEFKVVDDHDNPVPAGKTGELISRMIRGDTKVDYLGLPEESQ
                     DKTKGGWLRSGDMVHKDENGWYFFDFRKGTELRRFGDFIQPDFVESIIGRHPDVSEAC
                     VYGIPAASGSPGESDLVAAIAPFEDSVIDPASVYQKCKNELQGNFIPSYLHVVKEIPK
                     TITEKALTRVLREMFEKKEGDIYKYEDFK"
     gene            complement(10494..11162)
                     /locus_tag="PLR47_02425"
     CDS             complement(10494..11162)
                     /locus_tag="PLR47_02425"
                     /inference="COORDINATES: protein motif:HMM:NF021439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HOG11822.1"
                     /translation="MAIRDMNDLVQSGLDRSIELMTVAAHNSFRFNNKNTVKIVSLGK
                     EEIASIGEFCYSLADMSPLAARDGRFVMELIKEPCGLLLLGDKRKSDFNYNCGACGYK
                     TCAELNKAEVVESLTAWGPSCQFKNININIATNAAASMAWKLGLHCRVFSTLGMGAFA
                     LKMIEDVDLIVSVSVSAAKSDPYFDRHKYWTKEHWDETFQKEFPTFTRGFIGAVEDEG
                     TFKI"
     gene            complement(11191..11868)
                     /locus_tag="PLR47_02430"
     CDS             complement(11191..11868)
                     /locus_tag="PLR47_02430"
                     /inference="COORDINATES: protein motif:HMM:NF021439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HOG11823.1"
                     /translation="MSIEKSMKQFEQDRLEAVRIASRLLLASGTTTPRVGGVGECTIH
                     IIDDPCAIEDLCLGMESMSRENKAWDFFKRDAAMLRDADAVLVVTSLRCVNDPADINC
                     NMCGKMTCESLRETERLPDDADVAFKGPLCIFRANNISYAIDGIISQARNLGIDYGVF
                     WSVGAAAMRMKILPHATGFALAVAVSITEKSPFRDIPLKYGEINEHTMNDRIIKRLWP
                     QFRSIYS"
     gene            complement(11898..12284)
                     /locus_tag="PLR47_02435"
     CDS             complement(11898..12284)
                     /locus_tag="PLR47_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11824.1"
                     /translation="MAVFANTEKMYEVLGTLFNTLMNHPSVGPKFIESNITIKFTIND
                     PGGTIWLTPEKGVICGDADLKPTIDMTLSGDTCHQFWLQEVSMPIALAKGLIKAKGPL
                     PKVLKLLPLLKPAYEAYPEISRKYGL"
     gene            complement(12703..13314)
                     /locus_tag="PLR47_02440"
     CDS             complement(12703..13314)
                     /locus_tag="PLR47_02440"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG11825.1"
                     /translation="MSSNAQREVLRRAQIVDAALREISAKGMNNITVESIAKAAGLSK
                     GGVTHYFKSKDDICEAVFQSFFDFIFQRSKHNIDLYSDPLSKLLSFGWLYNWGDPDVS
                     TGYPLLFDFMAEACHKESYRAIFHEWVNNWVALLKDAIIEGNNDGTFNCSDPDATAKA
                     ISAVYHGLAVRWYLDRDSHSTQWTITSFTDAINGLLTGSLNKI"
     gene            complement(13504..>14204)
                     /locus_tag="PLR47_02445"
     CDS             complement(13504..>14204)
                     /locus_tag="PLR47_02445"
                     /inference="COORDINATES: protein motif:HMM:NF018652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11826.1"
                     /translation="IDEARNAGFQNIGLDLIYGVSGQDIRLWLKTLEQALSFAPEHLS
                     CYQLSLEEKTPLYKQYSKESLQLPAENEALDFFMNTSKILTDAGLIHYEVSNFARSDS
                     LKSRHNMKYWRHAPYLGLGPAAHSFLDSRRWWNKADVKAYLNEVGGGKKPVDKSESLS
                     AEQLALEALFLSMRTNDGIHLQQYKNRFGADLLVGKKQVIDELMENGLLELKDGRLQP
                     TLAGMAVADSLALM"
BASE COUNT         3205 a         3713 c         3807 g         3479 t
ORIGIN      
        1 caacaggctg gttcacggtc agatcctgga agcctgggtg ccgtttgtcg aagccaggtg
       61 catcatggtg gtggacgaca attccgccag cgatttcttc tgcgagacgg tgatccgcat
      121 ggccgtcccc agcgacattg aggtcaatat ctgctccgtg gatgattttg ccgccaatta
      181 cgcgtatgtc cggggaagcg gcaaaaagac catcgtcctg tttggcagga ttgccgatgc
      241 cttgaaggcc tacgagcagg gatttcattt tgaagcgctc aacatcggca atatctatca
      301 cgaagattac aaagtctgct gcgcgccgtc cgtgtttctg tgcgagccgg aaattgcgga
      361 cattatcagg ttgcagcggg aaaagggcgt ggccgtcgag gtgaaaaggg ttccccggga
      421 gaaggccgtg gatatacagg acgccctcaa agcgtcctgc gattcgaaac cgtagcgggg
      481 ggatcgcgtg ctgacacaaa tcgtcatcct gtccctggtc ggagggctgc tgtgcctgga
      541 ccggatcttc cttcaggcca tgctgtcgcg gccgatcatc atcgctccgg tcatggggct
      601 tcttctcggc gatgcctaca ccggcctcat catcggagcg attttcgagc tgttctggat
      661 ggaccgcgcc cccatcggca tttacattcc ccccaacgac tccattgcct ccgcgttcgc
      721 cgccgccctg gcgattctgg ccggccagtc cctgggcgcc gtcacgaaag aactgacggc
      781 tctctcggtt ctgctggctg ttcctttcgg tattctggca aaaaaaatgg atgtccgaat
      841 gatggccgca aacgaccttc tttccgaccg ggcgctggaa gcggcccgtt cacgggatct
      901 tcgcgcgata gaaaacatga cctgcctcgc gctgggccgc gcgtttgttc tttatgccgg
      961 cctgctgttt attctccagg tgattttcat tcccctcgtg attaatttgt acccgaagct
     1021 tccggtgcag gcaggcggcg cgctgaccct tacctactat tttctgccgg ttctcgggac
     1081 cgccgttgcg ctcagcacca tcaagctgcg cggagcggtt cccgtttttt gcgcgatctt
     1141 cctggcggcc gtcctggcca tggactattt tcatgtcttc tgacaagacc ggacagctct
     1201 tcagcgttga tctgatgaga gaggaagatc tgccggagat cctcgcgatc gaggccgcgt
     1261 cgtttgtgac gccctggacc aggggcatgt ttcaagacga gcttcgcctg ccccatgccc
     1321 agtgcctggt gatccgggcg cggcaggcgg agaaaacgca ggttgccgcc tacatcattt
     1381 tctggctggt ggccgacgag gttcatttgc ataatatcgc cgtccgcggc gagtttcgca
     1441 ggaaggggct ggcgtccggc ctgatgaacc tcatgcgcga catcgcgggg caggcggggg
     1501 cggaccgtca gacgctggag gtccgggaat ccaataccgg ggcaataaac ctttacggcc
     1561 ggtgcggttt tgtagtaaaa gggaagcgcc cccgttacta tgacgacacc cgggaagacg
     1621 cgctggtgat gtgggcggag gtccggcaga atggccatca tgaataaaca gatcaccatc
     1681 ggcagcgttt ccggaaatga cgaaatccgg cccggctatg ttttgatgac gattcatgtg
     1741 caccggtcgt ttcccgaacc ccggcccgga cagttcgtta tgatccgcat cgccggtttg
     1801 caggagccct ttctgtcccg tcctctcagc atcttctcgt tttcgcgcgg ccgatcgcat
     1861 ggccttgtaa agctgctgta ccgcgtggcc ggcaagggaa cgcagattct cgccgggctg
     1921 attcgtggat cgcaggtgga aatagtaggg ccgctgggca aaggctacac cgtggatcca
     1981 tcacgggaaa accgtgtgct gattgccggg ggcatcggcg tggcgcctct gtcgcttttg
     2041 gcgcagcatc tctgccgact gtcgggccgc ggcgcaaatc ccgctgtcgt gtatctgggc
     2101 gcgaagacgg cggatgagct cgtgggcctt gatcagcttc acaaatattg caacagcatt
     2161 cgggtctgca cggacgacgg ctctttgggc gagaaatccc ttgttacgag gacgtttcag
     2221 aaagacatga aaaaacacga tccggcaagg acgtccatct atgcctgcgg gccgaagccg
     2281 atgcttcagt cgctggcggg cattctgaaa gatcgctact tctgtcaggt gtcgctggaa
     2341 gaacgcatgg cctgcggtgt cggcgcctgc atgggctgcg ccgtggccgt gagagatgaa
     2401 aatggagaca agacttaccg gcgcgtatgc gcggacgggc cggtctttga tcttcagaaa
     2461 gtgatttggg aatagttttt tgccttttgc aatggaaaca ctcattggtt ttcccatgaa
     2521 ccagcattcg ggggtcgtca gttgaaacgt acgcctacaa cagatgcagc aatggttgtg
     2581 gaaatcgccg gcctcaaatt gaaaaatccg gtcatgacgg cctccggcac cttcggctgc
     2641 ggagaagagt atgccggtta tgtggatttg aaccggctgg gcggcatcgt cgtgaaaggt
     2701 ttgtcgctga aaccccgcct gggcaatcct ccgccccgca tcatggaaac cgccggcggg
     2761 atgctcaacg ccgtgggact gcagaatatc ggcgtggatg cgttcatcga ggaaaagctt
     2821 ccctttttgc gaacgtatga cgtggccgtc atcgccaaca tctacggcga gagctgcgcg
     2881 gagtatgcgc aggttgcccg aaaactcagc gccgcgccgg gtgttcacgc gctggaagtc
     2941 aatgtttcct gccccaatgt caaaaaaggc ggcctgagct tcggcgcaga cccgaaagcg
     3001 gcggcggaag tgacaaagcg cgtcaaggcc gaaacgcatc tgcccgtgat cgtgaaactc
     3061 acccccaacg ttacggatat tacggttatt gcgagggctg tggaagaagc gggcgcggat
     3121 gccgtctccc ttatcaacac actgaccggc atgtccattg atcttaaaac cagaacccct
     3181 catttgaaaa acattaccgg aggcttgtcg ggcccggcca tcaagcccgt ggcgcttcgc
     3241 atggtctggc aggcggcgca aaaggtgacg attccggtca tcggcatggg cggcatcatg
     3301 acggccgaag acgctctgga atttatggtg gccggagccg ccgccgtgca ggtcggcaca
     3361 gccaatttca tcaatcccct cgccacaagt gaaatcgtgc aagggatgag gcaatatgtc
     3421 aaacatcaca aactgaaaaa cattacggaa atcgtcggaa cgttgaaagt ttaatttgcc
     3481 gcgacaacct ggggcatgtg gagcagccaa tgcggtttga aaacaccgtt gattgcaggg
     3541 cgggtctata gtaatcttca ggttatcgga ttcgctaaac atcgacttca taggcgaatg
     3601 tagggcgggt cttcagaccc gccgagccat tgcgccgttc tcatttggcg cgcctgaagg
     3661 cacgccctac gtttagtggg gcgccgtcaa aaccagggga aaatggaaat aggcaatgcg
     3721 caggggaact gaagaaatcc tgtctggtgt ttatctgatc ggcgggccga atgtaacgca
     3781 ggcggacgat gcggccgttt atctgatcga ttttaaaggc gagctggtgt tgatcgacgc
     3841 gggggcgggc ggaagtgctt cgcagctcgt ccgcaatatt gaaatgctgg ggctgaatcc
     3901 tgcgctggtc tctcatgtga ttctgacgca ctgccacatc gatcacatcg gcgctgcgcc
     3961 attcttccgc gaaaaataca aagcgaaaat cgtcattcac gagatggatg caaaagcgct
     4021 ggaaacgggc gattccgtcc gaaccgcggc caactggtac ggcacgacat ttccgccgac
     4081 gaagatcgac cggaaattaa ccggcgcacg ggagacgttg aaattcgaca atgatgaact
     4141 gcactgtctg cacacccccg gacatacgcc cggttccatt gccgtctatc tggaccggtc
     4201 gggccaacgg gtcttattcg gtcaggatat tcacggtccg ttccataaag cctttggttc
     4261 gaatattgag gcctggaaga aatccatgca aaccctgctt gcccttgaag ccgacatcct
     4321 gtgcgaaggc catttcggta tttttcggcc taaaaataaa gcaagagcct atatcgaaag
     4381 atatcttgaa gagtacgagt aagtactgtg cgattgacgc tttggcgaaa ttcaaaggtg
     4441 ggtgagaaaa tgagaaagac actgtttgtt ttcttcatgc tgttttgggc tgcaggcatt
     4501 accggtgcgg cacaagctgg aatatttgac gatattctga aaaagcttcc ggcggccaag
     4561 agcgccgcac cttcagccgg tctggatcag aaaacgacga tttccgggtt aaaggaagct
     4621 ctggtcatca gctcaaagaa tgccgtcaaa gccgtgtcaa aaaaagacgg ctatttcggc
     4681 aatcaagtgg tgaaaatact acttcccgat aatattcaga aagctgccga tgtggttgcc
     4741 aaactgggct atcagaaaca ggttgatcat ttgattctca gcatgaatcg cgcagcggag
     4801 gcggctgccc ctaaggccat tcctcttttt gcggatgcca tcaagtccat gtccatcgat
     4861 gatgcaagaa agattcttca ggggggtgat gcggccgcca ccgagtattt caaagaaaag
     4921 acgtcggcaa aactctatga ggagttcaag cctgtggtcg tggcaaatat gaacaaagtc
     4981 ggggtggcga aagcctacaa gaatatgacg gctccctatg aagcgcttcc ccttgtatcc
     5041 agcaagtcaa tcgatcttga tcgctatgtg accagcaaag ccctggacgg cctgtttctg
     5101 atcatcgcag aagaagagaa gaagatacgc accgatcccg cggcgagagt gacggatctt
     5161 ctcaagactg ttttcggcaa atgagggaca gcacccgttt attgacggcg ccccaaaagg
     5221 agcgaagaat ggagagccac tttgcaaggc tgtcatggat ccgtcaattt ccgtgatgag
     5281 ttccctttaa cggatatatc ctgaacagca tcgccgacaa gaatcaaaag gatcctttcc
     5341 ggttcatcga aggttgcttt tgacgcttcc taatttcctt tttctcttaa aaccaaagaa
     5401 ggcacgtagc tcgccaggcg acgcggaaat actctggtca aaacagatgc ggcaaaccag
     5461 ttttgaatgc cgggaatgta gatgggtttc ccctttttga aagcctttac tcccttgcgg
     5521 gcgatattct ccggggaact gaggccgctg atagaatacc accagagttt ttttggaaat
     5581 cctttcccgc gaagcatcgg cgtatccgta taggagggat taagggtgca gattttgatg
     5641 ccggaagatt ttatttcctg gcctaccgcc tcactcaggc tctgcacaaa tgccttggtg
     5701 gcgccgtaaa cggcatggtg cggtgtgggt tgaaaggccg aggcggaaac gacgttaagg
     5761 attctgccgg attttttctc tatcagcccg ggtaaaaata accgcatcag tttaaggtag
     5821 gcaatggcgt ttaccgtgat gacctcaagc tgtccatcca ggggtagatt tgcaaaatct
     5881 ccgtagagca tgattccggc gttgttgacc agaatgtcaa tagtcttgcc ggtttgttca
     5941 acctgtctat aaagcagttc cggtcccgaa gccagggaga ggtcaacggg gaattttttt
     6001 gttttgatat gatattgatg ttccagatag tcggcccaac gacaaagctg atcctcttca
     6061 cggggatggg aaccgagaaa gagattggat ccttcacggg caaaacactc tgcaagagcc
     6121 tttccaatgc cggaggcggc accggtaatc aggacgtttt tacctttgag tatcagcatt
     6181 ttgcctcttt ccattagccg aatcgtaagc cccttttttc tcatcgactg gcgactcgtc
     6241 gtgcaagggg agcaggcggg tttagaggga taaccccgcc tgctcatggg aaaacagcgt
     6301 atgcaacctt gtcgggggct tacatttttt ccaggatgtg aatgcacatg gccgcttctt
     6361 caacgccgat gttgccgccg ccgttttccg ccagaccaat ctttgcccct tcaacctgcc
     6421 tcttccctgc ctcgccgcgg agctgcataa caagctcatg gatctgggag agtcccgagg
     6481 cgccaatggg atgccctttg cattcgagcc ctccgctggt gttgatgggt tttttgccgc
     6541 ccagcgttgt cgctcccgat tcggcgaaag gtcctccctc gcccatggga cagaaaccca
     6601 tggactccgt ttgatgcagt tcgccccagg atgtggcatc gtggagttct gccagatcga
     6661 tatcctcggg tcccactcca gccatatggt atgcctgctt tgccagacgc tcgccgatgt
     6721 ccggagcgtt caaatcgcga tcggcgccgg tgcccaggac ggaagcccgg atttgcacgg
     6781 gccgggcaga cgtcattttt ttcaggaatt tttcggaaca aatgatcgtg gcagcggacc
     6841 cgtccccgac cggtgcacac atggcgcgcg tcaggggata ggccaccatc ttgtcggcca
     6901 gaacctcttc cgccgtcatg acttgctgat actgagaaag gggattgagg gatccgtgca
     6961 agtggttttt cgcgcaaatc acggcaagct gctgctgtgt tgagttgaag cgactcatat
     7021 gccagcgagc ccccatggcg tatgcatcca tgaagatgct ccttccttcc ccgggcgccg
     7081 attgaccggc agggattttc acggtgaagg ttttgctgat ttccatcaga ttttggatat
     7141 gctgctcgaa attgcccacg tccatgcagg ttccgtaagc gctcagtgaa agcattttgt
     7201 tcgggttggt gattttttcc gaaccgattg ccatagccac gtcatacatc cctgaacgga
     7261 ttcccgtgta cgcaaggtgc agcgcggtag aacctccggc acaggcgttt tcaacattga
     7321 cgaccggcac cttgccaaca cccatgcgcc gcatgacaac ctcaccccgg atggaatgct
     7381 gattgtcgaa cattccccag aaagtgttag agaagaagac ggcatcgagg tcttccctcg
     7441 caagccctgc atccttaagc gccaagtccg tggcctcatg ggccatagaa attacggttt
     7501 tgtcgggata tttgttgaac cggatcatcc ccacaccgat gatatagaca ttacccattt
     7561 gcatattcct ccttgagcag cgcagtaccg attccgattg ccagagaatc gttggagcca
     7621 tcttcgatca ttgaggccct tgcatcgcga aagagttttt cgatgggata ttccttgctg
     7681 acgccgttgc cgccgaataa ctgcacggcg tcgcttgcta cctcaaaagc ggcattcgta
     7741 cagaaaacct ttgcggcgac cgagtaggga acagcaggcg gggtggtgtt catattgtaa
     7801 atcatcgccg ctctggataa ttgtctggcc gcctgaacct tcgaaaacat gtcgaagatt
     7861 ttcttttgca cccattggtg attggagatg gttttaccgc cctgtaccct ttctttgcag
     7921 tactgcacgg ccaaatcgta ggcggcctgg gcgacgccgg taaaaaaggc gcccatgcag
     7981 gcattggctg ttgccagcgt gatgtccgtc atcatctcgt agctttcggg gtctataatc
     8041 atgtcttcgc cggggcagat ggcgtcgtcg aagaatatct ctccctgagg aagttctctc
     8101 tgccccattt tgtccaaagg ctttcctctg gtaacccccg gctgtgtcag gtctatgagg
     8161 caaatgccgc ctccgctcat gccctgggcg ctgtctatgt tgacgaagag ggcggcattg
     8221 ctcgcaatgg gaccattgga gacccaggcc gctttctgac cgttgatgat ccagtccccg
     8281 tttttggctt tgacctgctg ggtgatcttg ggatcatgaa agaaaggcgt gcccggcatg
     8341 agaatatcgg aaccgtgttc cggttcagtg atcgaccagc agctcatgat gctggcgtct
     8401 tcgcattcga caaaggggat aatgaattta tttaccagat gatcatcagg caccatgcag
     8461 gcgtaaaagg ccgaaaaaat gctgacgccc aaagatacgg tgaagccggc gcttccatag
     8521 gccagttctt caaaaaagat atgcacggac aggggatcca ggcccatgcc gccgtacgca
     8581 tcggaaatca gggcggtatg gtatttattc ttgtacattt gccgcatgca cttccaatag
     8641 agagaatcct tcgcgatcac ttcctccggc gacagcttgt caagctcaat ggacgccggc
     8701 cgcagcactt ccatggaaaa cttgtgtacg gattctttca gggcaatatg ctcgtctgtt
     8761 aagtccaggt ttaaatctct ataagccatg gttttctcct ttaatgatca taaagcttca
     8821 tggggttcat tgcgtccgca atttcgttgg catgataatg cggctatttg aaatcttcgt
     8881 atttgtagat gtcgccttcc tttttctcga acatctccct caataccctc gtcagcgcct
     8941 tttcggtgat cgtcttcggt atctctttca ccacgtgcag gtacgacggg atgaaattgc
     9001 cctgcagctc gtttttgcat ttctgataga cggaagcagg atcgatgacg ctgtcttcaa
     9061 aaggagcaat ggccgccacc agatcgcttt cgcccgggga gccggaagcg gccgggatgc
     9121 cgtaaacgca ggcttcgctc acatccgggt gccgtccaat gatggactcc acgaaatcgg
     9181 gctggatgaa atcgccaaag cgtcgcagct ccgtaccctt cctgaagtcg aagaagtacc
     9241 atccgttttc gtccttgtgg accatatcgc ccgatctcag ccatcctccc ttggtcttgt
     9301 cctgtgactc ctccggcagt cccaggtaat ccactttcgt atcgccccgg atcatacggg
     9361 aaatgagttc accggtcttt cctgccggaa ccgggttgtc atgatcgtcg acgaccttga
     9421 attccattac gccggggacc ggcttgccga acgatccgac aggaccctgg cctgtgggtt
     9481 tgtaggcaaa ccccccctcc actgcgccgt accactcgag aatctggacg ttgaagcgct
     9541 tttcaaagcc ttcccaaatg gccatgggcg ttccggcact gataacaacc ttgacgggat
     9601 tgtctccgtc atcgtgtttt tccggctcgt tatagatgcc cgccgccatc cctcccagga
     9661 gagagaagga cgtgcatcca tacttgcggc agatgtccca gattctgctt ttggtgaatc
     9721 tcgggctgaa aacggccttg acccccagac tgagggcggg aaaaagagtt acggcctggg
     9781 cgtttccatg ggtgagcgaa agaccattat agaggatgtc gtcggatttg tatttccaga
     9841 cgatactgtt caggatgtta aacagccccg tgcgattgtt cctgatcatg acccccttgg
     9901 gatctccggt tgttccggaa gtgtaaataa tctgcatggg atgacgcaca tccatgatct
     9961 gctgctccac cggtttccag gtgtcctttt ccatcacctc gttcaaggca tgatgctttg
    10021 cggaaacggc tgcattctgc cccggctgat aggcgacggt gatgaaccgc agggtcggca
    10081 catcggtgat gaccgcctcc aggtcttcca gacattcacc ggaaacaatg accgcccggg
    10141 ccttgcagtt gttaaggagg aacttaagcc gctgtcgctt gttgcgggga tccacgggaa
    10201 cggcaatggc gcccagagtg atccccgcca tcattccgca gacgaactcc ggatgatttc
    10261 tcatgtagat ggcgaacgta tcgcctttgc cgattccttt gtccataaaa agccgggcta
    10321 ttttattgga acgctcatga aggatgcgat aggtgaggat gtcttcaccg tgttcgccgt
    10381 tttcaaaaat gtagatctcc ttgtccggca tttcatccgc ccgaatttcg atcaaatggg
    10441 aagcgataac ctgattaaga tgtgcttttg atgccatgat ttattttccc ctgctaaatt
    10501 ttgaatgtgc cttcgtcttc cacggcgcct atgaatcccc tcgtgaaggt gggaaattct
    10561 ttctggaagg tttcatccca gtgttctttt gtccaatact tgtgccggtc aaaataagga
    10621 tcgctcttcg cggcgcttac cgacacactg acgatcaaat ccacatcctc aatcatcttc
    10681 agggcgaagg cgcccatccc cagggtgctg aagacgcggc agtgaagtcc caatttccag
    10741 gccatggagg ccgccgcgtt ggtcgcgatg ttgatattga tgtttttaaa ctgacaactg
    10801 gggccccatg ccgtcagcga ttccactact tcggccttgt tcagttccgc gcaggtttta
    10861 taaccgcagg cgccgcagtt gtagttgaaa tcagatttcc ttttatcccc caggagaagg
    10921 agtccgcagg gctccttgat cagctccatg acaaaacgac cgtcacgagc ggctaaaggt
    10981 gacatatcgg ccagggaata acaaaactcg ccgatggaag cgatctcctc cttccccagg
    11041 gatacgatct ttaccgtatt cttgttgtta aacctgaagc tgttgtgagc ggcgacggtc
    11101 atcaattcta tggacctgtc caagccgctt tgcaccagat cattcatgtc tcgaattgcc
    11161 atttttgcct cctgccattt gttgatatca ttagctatag atggatctga actgcggcca
    11221 cagcctcttg atgatccggt cattcatagt gtgttcgttg atctccccgt acttcagcgg
    11281 gatgtcccgg aagggactct tttccgtgat ggagacggct accgccaaag cgaatccagt
    11341 ggcatgaggc aaaatcttca tcctcatggc ggcggccccg acagaccaga aaacgccata
    11401 gtcgatcccc agatttcttg cctgggaaat gatgccgtcg atggcatagg aaatattgtt
    11461 ggcccggaag atacaaaggg gacctttgaa agcgacatcg gcgtcatcgg gtagcctttc
    11521 cgtttctctc agcgattcac aggtcatctt gccgcacata ttgcagttga tatcggccgg
    11581 atcgttgaca cagcgaaggg atgtaaccac cagcaccgca tcggcgtccc gcagcatggc
    11641 ggcatctctt ttgaaaaaat cccaagcctt gttttcacgg gacatgcttt ccatgcccag
    11701 acaaaggtct tctatggcgc atggatcatc gatgatatgg atcgtacatt caccgacgcc
    11761 tcccacacgg ggtgtcgttg ttccggaagc cagcagaagc cgcgaggcaa ttcttaccgc
    11821 ttccagcctg tcttgctcga attgtttcat gctcttttct atgctcatac cccccccctt
    11881 gcttgagtga aacgttttta tagaccgtac tttcgggaga tttccgggta ggcctcgtag
    11941 gccggtttca aaagtggaag cagcttcaat actttgggaa gaggcccttt agccttgatc
    12001 aaacccttag ccagggcgat gggcatcgat acttcctgca gccagaactg atggcaggta
    12061 tctccgctca gggtcatgtc aatcgtcggt ttaagatcgg catctccgca gataacgcct
    12121 ttttctggcg tcagccagat ggttccgccg ggatcgttga tggtaaattt gatggtgatg
    12181 ttggactcga tgaatttcgg tccgacagaa gggtggttca tgagcgtgtt aaaaagggtg
    12241 ccgagaactt catacatttt ttcggtattt gcgaatactg ccataatact tccctccttc
    12301 atctgtcttt tattggtggt taagtaaaaa caatcgttcg cttcatccgc ctctgaaaca
    12361 actacccctc cttgatttat ttttcagtta tggacgcaca atcaaagatc tgtgatctgt
    12421 tgtgcaatag cctttgctga tagacaaaac aaaaaaacaa tggaactaaa attcttgaga
    12481 tccggcaatg aacttgctga agaaatttat aatccgttta acgtgagagg gaagggagat
    12541 ccagagatcc gaaggagata ggtgttgtct tagaccgtgg aggaaatcag ccctgaggtc
    12601 agagcaatat cgtgtgaaaa atggcagaca ggaactattt tgggcgttct gaatcacgat
    12661 attctccctg gcaaatatta tggcaaacat tgagccgcga agttatattt tgttcagact
    12721 gccggttaat aaaccgttaa ttgcatccgt gaatgatgtt attgtccact gcgttgaatg
    12781 actgtcccgg tcaagatacc agcgaacggc taatccatgg tatacggctg atatggcctt
    12841 tgctgttgca tcgggatccg aacaattgaa ggtgccatca ttattgcctt caatgatggc
    12901 atctttgagg agagccaccc aattatttac ccattcgtgg aatattgccc tgtagctttc
    12961 tttgtggcat gcctcagcca tgaaatcgaa gagcagcggg tagccggtcg aaacatcggg
    13021 atctccccaa ttatagagcc agccaaagga aagcaatttg gagagaggat ctgaatataa
    13081 atctatgtta tgtttgcttc tctgaaaaat gaaatcaaaa aatgattgaa aaacggcttc
    13141 gcagatatca tccttggatt taaagtaatg ggtgacgcct ccctttgaaa gccccgctgc
    13201 tttagcaatg gactcgaccg tgatattatt cattcctttg gccgaaattt cccgtaaagc
    13261 tgcgtcaaca atctgagccc gtctgaggac ttctcgttgt gcattggaag acataaaacc
    13321 tccttccttg ctaaaccaaa aaaccgacca gtcggactaa accaaactga tttagcgaat
    13381 atgccatgca aaatatatag tcaatactac tttatgttca ttttttataa aaaggtgctg
    13441 taccgtcaag ggggtcgatc gacgtctgtc aagctgcaat ccgggtcatg acggaaaaga
    13501 tcgctacata agcgccaggc tgtccgccac ggccatgccg gccaaggtcg gttgaaggcg
    13561 gccgtctttc agctccagaa gaccgttttc catcagttca tcgatgactt gcttcttgcc
    13621 cacaagaagg tccgcgccaa agcgattttt atattgctgg agatggattc cgtcattagt
    13681 gcgcatactc aggaagagcg cctcaagggc gagctgctcc gcagagaggg attctgattt
    13741 gtccacaggc tttttccctc cgccgacttc gttcagataa gccttcacgt ctgccttgtt
    13801 ccaccagcgc ctggaatcga ggaaagaatg cgcggccggc cccaaaccca gataaggcgc
    13861 gtgccgccag tatttcatgt tgtgtctgga ttttaaggaa tcggagcggg cgaagttgga
    13921 tacctcgtaa tgaattaaac cggcatccgt caggattttc gatgtgttca taaagaaatc
    13981 cagtgcttca ttttccgcgg gcagttgcag gctttcttta ctatattgtt tatacagagg
    14041 cgttttctcc tccagggaga gctgatagca ggaaagatgc tccggtgcaa atgacagggc
    14101 ttgctccagg gttttgagcc aaaggcgaat atcctggccg gaaacgccgt agatcagatc
    14161 gaggccgata ttttgaaatc cggcgtttct ggcttcgtcg atcg
//
LOCUS       DAOSIM010000028        14188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_4380_length_14188_cov_8.604967, whole genome shotgun sequence.
ACCESSION   DAOSIM010000028 DAOSIM010000000
VERSION     DAOSIM010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 14188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14188
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4380_length_14188_cov_8.604967"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLR47_02450"
     CDS             complement(<1..399)
                     /locus_tag="PLR47_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG11827.1"
                     /translation="MYEQKPWLKYYGNVPHSIDYPRVTMYEALLQTAREKADALAYDF
                     MGYTSTYNRFVSEIDRCADALAALGLKQGDRITISMPTSPQGIIAFYAANKLGAVASM
                     IHPLSTAKEIEFYLNVAKSRFALTLDAFYAK"
     gene            complement(488..>1108)
                     /locus_tag="PLR47_02455"
     CDS             complement(488..>1108)
                     /locus_tag="PLR47_02455"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG11828.1"
                     /translation="PRVFARFKMKLDPMFSELDRYVQNPRRIMDIGCGFGVPAAWLLE
                     IFPQAEVFGLEPDEERVLIANRAIGARGRVQVGRAPDLPDAEDPVDYVLMLDMLHLID
                     DHELQLVFRRICGKLGPDGTLVIRATVPSAGKVPWKRWIETARLQWTGMPKRFRVERG
                     ITGFMEAAGFDVAVHASPVPGVEEKWFIGKKREEVSSPSWRIPEKK"
     assembly_gap    1109..1158
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1159..3495)
                     /locus_tag="PLR47_02460"
     CDS             complement(<1159..3495)
                     /locus_tag="PLR47_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOG11829.1"
                     /translation="MVLVTALIVGILFCWETSRLKIETDILESMPHHDPVLASARRVI
                     THLPVSDRLFIDLEQTSSDRDQLVEAARLVEDRLSKSGLFERVGIADEAGAFPELMAH
                     VREHLPSLFGTEDLERKIAPLLAPEKIRETMAENRISLGELQGIGRAEMISRDPLGFS
                     PVILERMSSLSPAGSAQFYSGRLLSSDGRHALIIARLAGSGTDTTRAAQISGLMDDCA
                     RMLEARRDSNGRFTLTPVGAYRAALDNETVARRDTRLAVLLTTLGIALLLILSFPRPL
                     IGLLALLPSAVGAIAALFACSFLFKSMSMLAVGFGGAIMAFTVDLGLTYLLFLDQPRA
                     TFGREAAREVWSAEFTAALTTICAFLLLLISDFDILAQIGVFAALGITFAFLFVHFVF
                     PIIFPEMPPARRASNRLLTGAVQKIAAPASWKLIAALVFGLTMLLFAKPVFNVDLQAM
                     NSVSSATLAAEKKLQDTWGNLSGRLYVLLEAPDLERLRKKNDQLEALMAREVKGGRLA
                     PAFLPTVLFPTPESAARHFAAWRSFWTPERVGALRTNLEKAAAENGFAPDAFDPFWTV
                     FQAQRPGVFTIPPKHLEMLGIARTSEGFAQLTPMAAGKHYNASEFFDRLAAAGEIDLF
                     DADFFNRRMGEFLKSIFIEIALIVSLGLIAVVFLFFLDWRLSLAVLAPVAFALISTLG
                     TLKLIGRPLDIPGIMLWVVILGMGVDYAIYYTCTYQRHPEEASPAMNTVKLSIFLSAA
                     TTFIGFGVLTLANHSLLRSIGLVSLLGIGYSLLGAYFIL"
     gene            complement(3644..4417)
                     /locus_tag="PLR47_02465"
     CDS             complement(3644..4417)
                     /locus_tag="PLR47_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG11830.1"
                     /translation="MSHKKRTANEQKDAKNRRRAARNLGRFAFIIPAYNHGATVAQVA
                     GSARDMGFPVFVVDDGSTDDTAMRLAEVQGIEVLRHEKNEGKGAAILTGFMAASHKAD
                     YAITIDADGQHDPEDAIHLIDAIPLKKRPIVVGAREGMDAVQGAHIPWTSRFGRKFSN
                     FWVRMSGGPKISDTQSGMRIYPLPESLSLPVRARRFQFEVEILVQARRIGIPIIEAPI
                     RVLYLPDGARISHFRPFVDFLRNAKTFTRLMFTRVFGRH"
     gene            complement(4438..6150)
                     /locus_tag="PLR47_02470"
     CDS             complement(4438..6150)
                     /locus_tag="PLR47_02470"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOG11831.1"
                     /translation="MENLFKQSFVAGVERVAAWAELLDAINVYPVADGDTGRNLSISL
                     SPLRRADGEKDKIVRRLLLAARGNSGNIASQFFSVFYAADFSGSLKETVRQANEKAWK
                     AVSDPRPGTMLSVFDALEEALGGEEGVPGKEGVDRIMSRLEKAVRETRETLPKLRKAG
                     VVDAGALGMYIFFEGFFRTLAGQDHAYRPVTEIFPGFLAISPSFHENLEAGYCIDFVV
                     KADAPSEDLAKIAAGQESAVILRDGDLYKIHLHTDDREKIRSRMGALGSVMAWEDDNL
                     ALQIRDFMNTPADAALHIMTDAAGSLTRDDARKHGFTLLNSYLNVGDQSMPETYFHPD
                     ELYRAMSAGVKVSTSQASVFERHECYASVLARFEKVLYLCVGSVFTGNYSVAMEWKKE
                     NDPENRLTVIDTGAASGRLGVMVLAAQRRLLRTKDMGRTFAFARDAVARCEEYVFLEK
                     LQYLAAGGRLSKTGAFFGDMLKMKPVISPQPEGAKKMGVVRNRADQIKMALDKMAAVL
                     SPDSLALIMLEYSDNIEQVSEFRKQVQKLYPRAEIILQPLSLTSGAHMGPGTWGIAFL
                     KMEE"
     gene            complement(6151..6567)
                     /locus_tag="PLR47_02475"
     CDS             complement(6151..6567)
                     /locus_tag="PLR47_02475"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOG11832.1"
                     /translation="MSTGRNSFEAEICVPFFDLDPMQIVWHGNYLNYFEIARAGLFEH
                     YGVDLYAYYEREKIIFPIIRTATKHIFPLRHRDEIIVKATLADANIKLVVDFEIRMAT
                     DGSVCARGRTEQVAVKVPEMETLFSIPRDIRNLLGF"
     gene            complement(6564..8117)
                     /locus_tag="PLR47_02480"
     CDS             complement(6564..8117)
                     /locus_tag="PLR47_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HOG11833.1"
                     /translation="MKKIGAKAALVIDGGPVRFEEIIAVAIDGLPVRISRSRAFLKRM
                     SATEKALMTSLENGVAVYGVNTGYGKSCGNRISLAAAMKNGPNILKFHGCGTGEPIGI
                     EETRAAMLARILCLARGYSGASVGLLEQMAAFLNKGITPVVPCEGSVGASGDLTPMSY
                     IGAALAGRRDVFYEGDVMPAAKAIKKAGLKPYVYRPKEPLSMVNGTTTMTGIAAVCIE
                     RSRRILDAVVYATALSVHAMKGNAHHYHPVISEAKPFPGQAAVAREIARLLTSKVSAR
                     RLEDDALETLQDPYSLRCAPQALGVLADALSWIIPWVEIEANSSNDNPIFDPATGQVL
                     MGGNFYGGHIAFAMDGLKAALASAADMCDRQIMLLVNPNVNRGLPGDLVRKNGASQGL
                     HHGFKAMSIASSALAAEALKATMPAASFSRSTESHNQDKVSMGTIAARDAERVCTLAE
                     RVAAIHLLAAAQACDIRAHVKVRPLLDKAVRNIRQVSDPVIEDRPLDGDIERMCAAIR
                     YTDFFLGET"
     gene            complement(8150..9379)
                     /locus_tag="PLR47_02485"
     CDS             complement(8150..9379)
                     /locus_tag="PLR47_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11834.1"
                     /translation="MKFLLIYPKWEKLDRQTRFILPPHGPVVMAAALPSDAEVAFVDE
                     NVEPVDLGADADFVGVSMMLTAQVKRGWEIADAFRARGIPVICGGIAVMLHAEECAKH
                     ADAVFLGEAEGRMEQVFADFQAGKLQKVYNYMQNFPPIENVGTARRGILKREMYNYRG
                     VQMVDLVHASRGCRFNCYPCAVRFLGGRSFRPRPYDRVMEEMASIDNNRLFIVDNSLA
                     QDRDWEIGLFKEMIPLKKKWCCHPIEDDDEVLDLAAQAGAWFVYQTIFDSSDYIRDRI
                     KRYHDHGIAVEGSILLGLDYHTEDYIKRWMDFLLEIELDMAEFTILTPFVHTRTFDDL
                     HGEGRILSCDWNDYTCGKVVFQPKMMTPEKLQEMYYYCWDRFYEEEPANYKMFKMLKQ
                     VMEKEKADGTYKPRVRA"
     gene            complement(9393..10640)
                     /locus_tag="PLR47_02490"
     CDS             complement(9393..10640)
                     /locus_tag="PLR47_02490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOG11835.1"
                     /translation="MSEHRDFPDNRRVVITGLGMATPLGTGREEFSRRLFQGESAVDA
                     VQAFDTGAAASHLGAEVRDFQPRDFISIKNLRRMDRLSMLATASARLALSDAGIAVTA
                     ENRDRVGILFGTAFGATDVTVQFEDALLAQGPGAANPILVPNTVMNAPAGYASIELGF
                     RGVNVTVTHFAVSAETAVAYAASEIRRGTADVMLAGGADILSKFYYEALSKFRGLSPD
                     DGGPEGAHPFDERRNGYVAGEGCGLLCLESLACARERGAKICCEITGAGLGSSPAPPT
                     AWPRDPEGFCRAFLRALKQARIDVADVSAVMAAANGSRVLDAVEAAAYEGLFASRAVR
                     PWVTSIKGALGESFSGGGIRACALALSVERGCLPLTVGLERPAAPLNFVAGKKVKTHL
                     KHTVLSGISFGGACACLVFSRCE"
     gene            complement(10633..11877)
                     /locus_tag="PLR47_02495"
     CDS             complement(10633..11877)
                     /locus_tag="PLR47_02495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOG11836.1"
                     /translation="MKKRIVITGMGTKNAIGGNVSEFARSLREGVCGISQIDLFDTTD
                     FRTHKGGQVKDFDPRRSIPEEFSLKRISRADALSMAAALEALTDAGLYPLQENIKENM
                     GIAIGGGAGGLLEAESFYADLLRKGKERSKYSQLSSLFCASSADRIASSLEFSGPRAT
                     FMTACSAGATALGYARDLILNGHAGIMLAGGVEPMCRLTYAAFNALKSVDEDVCRPFD
                     RNRSGLSLGEASAVLVLEPLETAQNRGAKIYGELMGYGVTCDSFHMTSPDEKASGAAR
                     SMRAALADGGLTVEDIDYINAHGTATPVNDLTETKAIKEVFGSRAYQIPVSSTKSMHA
                     HTLGASGALEGIVSLLALSKRFVPPTIHWRERDPECDLDYVTQGARRAELRAVLSNSF
                     AFGGNNTTVIFGRYEGQGAIHE"
     gene            12075..13124
                     /locus_tag="PLR47_02500"
     CDS             12075..13124
                     /locus_tag="PLR47_02500"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOG11837.1"
                     /translation="MFKTRMTEMFGIRHPIMLAGMNWITEPKLVSAVCNAGGLGILAI
                     ARCNPKETRENIKQIQDLTDKPFGVNQILVGPGAKENIAVAIEEKVPIINYSLGKPWF
                     VNDVHAYGGKVVGTIAIARHAAKAVQLGCDALVITGHEAAAHGAAATSMVLIPIVASM
                     VKVPLIAAGGFYDGRGLAAALALGADGISMGSRFMVVKESMVHENFKRLCMEATEQDT
                     LYDNVFDGMDGRVLKTKEALAMMKRGFPIVEAFKGALLVKRLMNLSFAKFLGLSLEML
                     RAEEGHPLMVLARQAVGNMKHLKALNDGDVEGGILFAGQCCGGITDNPTTKELIDRII
                     REAEEAQARLNSCRT"
     gene            13185..13796
                     /locus_tag="PLR47_02505"
     CDS             13185..13796
                     /locus_tag="PLR47_02505"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOG11838.1"
                     /translation="MQLTLIRHGETVWNREKRVQGLTDVELSETGIDQARRLALSLRD
                     FPIDVIYSSPLKRAFQTAELINRYHRKAINVEPGLMEMDQGDFEGLTFQELLAREQDF
                     IRSWIADPESVRMPGGESLAELQSRAWPPVEKLMAEGRNALIVSHNFTIATILCRLRN
                     IRLSEFRSTCVDNASRTHIRIEEGAARIEAINDVRHLKTETDV"
     gene            complement(13980..>14188)
                     /locus_tag="PLR47_02510"
     CDS             complement(13980..>14188)
                     /locus_tag="PLR47_02510"
                     /inference="COORDINATES: protein motif:HMM:NF020839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family DNA-binding protein"
                     /protein_id="HOG11839.1"
                     /translation="KIMGMTLSEMQELESMWSYEKSNEKLLRRLLELLNNHLKRLDDR
                     IADLDILRHEILEYQERISAKINK"
BASE COUNT         3006 a         4301 c         4087 g         2744 t
ORIGIN      
        1 cttggcgtaa aaggcgtcca gcgtgagcgc gaagcggctt ttggcgacgt tgagataaaa
       61 ttcaatttcc ttggccgtgg aaagaggatg gatcatgctg gccaccgcgc ccagtttgtt
      121 ggcggcgtaa aaagcgatga tgccctgcgg ggaggtgggc atggaaatgg tgatgcggtc
      181 gccctgcttc agacccagcg cggccagggc gtcggcgcac cggtcgattt cggaaacgaa
      241 cctgttgtag gtggatgtat atcccatgaa atcgtaggcg agggcgtcgg ctttttcacg
      301 cgccgtctgc aaaagcgcct catacatggt gacccgcgga taatcgatgg aatgcggaac
      361 gttcccgtaa tacttcagcc agggtttctg ctcgtacatg gtcgaacctc cttagaattt
      421 tatggactgc tgcttcgtcg gcggccctgc cgcggcaagg ccgccgctca tccagtatcc
      481 ttgtccgtta tttcttttcc gggattcgcc aagaaggact ggaaacttct tcccgctttt
      541 tcccgatgaa ccacttttct tccacgccgg gaaccggcga ggcgtgcacc gcaacgtcaa
      601 agcccgccgc ctccatgaag cccgtgattc ctctttccac gcggaagcgc ttcggcattc
      661 ccgtccactg caaacgggcc gtttcgatcc atcttttcca cgggactttt ccggccgagg
      721 gaaccgtcgc ccgaatcacc agcgtcccgt ccggcccgag cttaccgcaa atgcgccgaa
      781 aaacaagctg caattcatga tcatcgatca ggtgcagcat gtccagcatc agaacataat
      841 cgacggggtc ttccgcgtcc ggcagatccg gcgcccgccc aacctgcacc cggccccgcg
      901 cgccgattgc gcggttggca atcagcactc tttcctcgtc cggctccagg ccaaacactt
      961 ctgcctgcgg gaatatttcc agaagccagg cggccggcac gccgaagccg caaccgatat
     1021 ccatgatccg gcgggggttt tgcacatatc gatccagttc ggaaaacatc ggatccagct
     1081 tcatcttgaa ccgggcaaaa accctcggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnca aaatgaaata cgcgcccagc agggaatacc cgatgcccag
     1201 aagcgaaacc aggccgatgc tgcgcaggag ggagtgattc gccagcgtca gaacgccgaa
     1261 accgataaac gtcgtagcgg cggaaagaaa gatggagagc ttgacggtgt tcatcgcggg
     1321 agaggcctcc tccggatgac gctgataggt gcaggtgtag tatatggcgt aatccacgcc
     1381 catgcccaga atgacgaccc acagcatgat gccgggaatg tccagcggac ggccgatgag
     1441 cttgagcgtt cccagcgtgg agatgagcgc aaaagccacg ggggccagaa cggccagcga
     1501 caggcgccag tccagaaaaa acaaaaatac caccgcgatc aggccgaggc tgacgatcag
     1561 ggcaatttcg ataaaaatgc tcttgagaaa ttcccccatg cggcgattga agaaatccgc
     1621 gtcgaacaga tcgatctcgc cggctgcggc cagacggtca aaaaattccg aagcgttgta
     1681 atgcttcccg gcagccatcg gcgtgagctg cgcgaagcct tcggacgtcc gggcaatgcc
     1741 cagcatttcc agatgtttcg gtggaatcgt aaagacgccg ggccgctgtg cctgaaagac
     1801 tgtccagaag ggatcgaacg cgtccggcgc aaagccgttt tccgcggccg ccttttccag
     1861 atttgtcctg agcgccccga cgcgctccgg cgtccagaag gagcgccagg cggcaaaatg
     1921 acgggcggcc gactccggcg tgggaaacag aacggtcggc agaaacgccg gggccagtct
     1981 gccgcctttc acttccctgg ccatcagcgc ttcgagctga tcgtttttct tccgcagccg
     2041 ctccaggtcc ggcgcctcca gaaggacata caggcggccg ctgagatttc cccaggtgtc
     2101 ctgcaatttc ttttcggcgg caagcgttgc ggagctgacg gaattcatgg cctgcagatc
     2161 cacgttgaag acgggtttgg cgaaaagcag catcgtcagt ccgaagacca gcgcggcgat
     2221 cagtttccat gaagccggcg cggctatttt ttggacggcg cccgtcagca ggcggttgga
     2281 tgcgcgacgg gcgggcggca tctccggaaa gataatggga aaaacgaagt gaacaaacag
     2341 aaaagcaaag gtgatgccca gcgcggcaaa aacgccgatt tgggccagaa tgtcaaaatc
     2401 gctgatcagc agcagcaaaa aggcgcagat cgtggtcagg gcggccgtga attcggccga
     2461 ccagacttcc cgcgccgcct cgcgcccgaa cgtggcacgc ggctgatcca gaaacaacag
     2521 ataggtgagg cccagatcga cggtaaaagc catgatcgcg ccgccgaagc ccaccgccag
     2581 catcgacatc gacttgaata aaaaagagca ggcaaacagg gcggcaatgg ccccgacggc
     2641 tgaaggaagc agcgccagga ggccgatgag cgggcgggga aaagacaaaa tcagcagaag
     2701 cgcaatgccc agcgtagtga gcaaaaccgc cagccgcgtg tcgcgcctgg cgacggtttc
     2761 gttgtccagc gccgcgcggt aagcgcccac cggcgtcagc gtgaatcggc cgttcgaatc
     2821 ccggcgcgcc tccagcatcc tcgcgcaatc gtccatcagc ccggagattt gcgccgcccg
     2881 ggttgtatcc gttccggagc cggccagccg cgcgatgatg agcgcgtggc ggccgtcgga
     2941 agacagaagc cggccggaat aaaactgggc ggaaccggcc ggcgagagcg aggacattcg
     3001 ctccagaatc accggggaaa atcccagcgg atcccgggag atcatttccg cacggccgat
     3061 gccctgaagc tcaccgaggc tgatccggtt ttccgccatc gtctcacgga ttttttccgg
     3121 cgcgagaagc ggcgcgattt tccgctcgag atcctccgtg ccgaacagcg acggcagatg
     3181 ctcccggaca tgcgccatca gctccggaaa cgcacccgcc tcatcggcga tcccgactcg
     3241 ttcaaacaag ccgcttttgc ttaaccggtc ttcaacgagc cttgccgcct cgaccagttg
     3301 gtcgcggtcg gaggaggtct gttcaagatc gatgaaaagc cggtcggaaa cgggcagatg
     3361 agtgatgacc cgccgggcgg aggccagcac cgggtcatgg tgcggcatgg attcgagaat
     3421 gtcggtttca atttttaaac ggctggtttc ccagcagaac agaatcccca cgatcagagc
     3481 ggtgaccaga accatcgacc atcttatggg atatcggcga ggcggcatga agaccttcag
     3541 attgtcggga ttattttcct gctttctttg ctgtttttaa accacatttc ccaaaggctg
     3601 gcaagaaaaa accgggcatg gaatcaggga ttggcctgtg cggtcaatga cggccgaaaa
     3661 cacgggtaaa catcaggcgg gtaaaggttt tggcattgcg aaggaaatcg acgaatggac
     3721 gaaaatgaga gattcgcgcc ccatccggca ggtaaaggac ccgaatcggc gcttcgatga
     3781 tcggaatgcc gattcttctg gcctgcacca gaatttccac ttcaaactgg aaccgcctcg
     3841 cccggacggg aaggctcagg gattccggaa gcggatagat gcgcatgccg ctctgggtat
     3901 ccgaaatttt aggcccgccg gacattcgaa cccagaaatt ggagaatttc cggccgaagc
     3961 ggctggtcca gggaatgtgg gccccctgca ccgcatccat cccttcgcgc gctcccacca
     4021 caatgggtct ttttttgaga ggaatggcat cgatcagatg gattgcgtct tcgggatcgt
     4081 gctgaccgtc cgcgtcgatc gtaatcgcgt aatcggcttt gtgcgaggcg gccatgaaac
     4141 cggtcaggat ggccgcgccc tttccttcgt ttttttcatg gcgaagcact tctatgccct
     4201 gaacttccgc cagccgcatg gccgtatcat cggttgatcc gtcatccacg acaaaaacgg
     4261 ggaatcccat gtcccgggcg cttccggcca cctgggccac cgtcgcgcca tgattgtagg
     4321 cgggaatgat gaaagcgaac cggccgagat ttcttgccgc cctccgcctg tttttcgcat
     4381 ccttctgttc gttcgccgtc cgttttttat gactcatcac tttccactcg tcacttttca
     4441 ctcttccatc ttcaagaacg cgatgcccca cgttccgggg cccatgtgcg cgccggacgt
     4501 gagcgacagg ggctgaagga taatctccgc acgcggatac agcttctgca cctgctttcg
     4561 gaattcggaa acctgttcga tgttgtccga atactccagc atgatgagcg ccaaagagtc
     4621 cggagaaagg accgcggcca ttttgtcgag cgccattttg atctgatcgg cgcggttgcg
     4681 gacgacaccc attttttttg cgccttccgg ctgcggggaa atcaccggct tcattttcag
     4741 catgtcgccg aaaaaagcgc cggttttgga aagccggccg cccgcggcga ggtactgcag
     4801 tttctcgagg aacacatact cttcgcagcg cgcaaccgca tcccgggcaa aagcgaacgt
     4861 ccggcccatg tctttcgtcc gcagcaggcg cctctgcgcc gcgagcacca ttacgcccag
     4921 ccggccggag gcggcgcccg tgtcgatcac cgtcagtcga ttttccggat cattctcctt
     4981 tttccactcc atcgccacgc tgtaattgcc ggtaaaaaca gagccgacgc acaggtagag
     5041 aactttttca aaccgggcaa gcacgctcgc gtaacactcg tgtctttcaa acacggaggc
     5101 ctgtgacgtg gacaccttca cgcccgcgct catagccctg tagagttcgt cggggtgaaa
     5161 atacgtctcg ggcatcgatt gatcgcccac gttgagatag ctgttcaaaa gggtaaaacc
     5221 gtgcttcctg gcgtcgtcgc gggtgagcga ccccgccgcg tccgtcatga tgtgcagggc
     5281 ggcatccgcc ggagtgttca tgaaatcgcg gatctgcagc gcgaggttgt cgtcctccca
     5341 ggccatcacc gaacccagcg cgcccatccg cgagcggatc ttttcccggt cgtccgtgtg
     5401 cagatgaatt ttatacaaat cgccgtcgcg caaaatgacg gcgctttcct gtccggcggc
     5461 aattttcgcc aggtcttccg aaggcgcatc ggccttcacc acaaagtcga tgcaataccc
     5521 cgcttcgagg ttttcgtgaa acgagggcga aatggcaaga aagcccggaa agatttccgt
     5581 caccggacga taggcgtgat cctgtccggc cagtgtccgg aaaaagcctt caaaaaaaat
     5641 atacatgccc agggcgcccg cgtccactac gccggccttt ctcaatttgg gcagggtttc
     5701 cctcgtctcg cgaacggctt tttccaggcg cgacatgatc cgatccacgc cttctttgcc
     5761 gggaacaccc tcctcgccgc caagagcctc ttccagcgcg tcaaagacgg acagcatggt
     5821 tcccggccgc gggtcgctga ccgccttcca ggctttttcg ttcgcctgcc gaaccgtttc
     5881 cttaaggctt cccgaaaaat cggcggcata aaacaccgaa aagaactgcg aagcgatatt
     5941 gccggaattg ccgcgcgccg ccagcagcag ccgccggacg attttatctt tttcgccgtc
     6001 ggcccggcga agaggggaaa ggctgatgga aagattccgc cccgtatccc cgtccgccac
     6061 cggatacaca ttgatcgcat cgagcagttc cgcccaggcc gccacccgct ccacgcccgc
     6121 cacaaaggat tgcttgaaca gattctccat ttaaaatccc aataaattcc gaatatcccg
     6181 cggaatgctg aagagcgttt ccatctccgg cactttcacc gccacctgct ccgtgcgccc
     6241 gcgcgcgcag acgctgccgt ccgtggccat gcggatttcg aaatccacca ccagcttgat
     6301 gttggcgtcc gcgagcgtcg ccttgacgat gatttcgtcc cggtgtctca gaggaaaaat
     6361 gtgctttgtc gccgtccgga tgatgggaaa aataattttt tcccgctcat aatacgcata
     6421 gagatcgacg ccgtaatgtt caaagagacc cgcgcgggca atctcgaagt agttgaggta
     6481 attgccgtgc cacacgatct gcatgggatc cagatcgaaa aacggcacgc aaatttcagc
     6541 ctcgaagctg ttcctgcctg tgctcatgtt tcccccagaa agaaatccgt atagcggatg
     6601 gccgcgcaca tccgctcaat gtccccgtcc agcggccggt cctcgatcac cggatcgctg
     6661 acctgccgga tgttgcgcac ggctttgtcc agcagcggcc ggactttgac gtgggcgcgg
     6721 atatcgcagg cctgcgccgc cgccagcaga tgtatggccg ccacccgttc ggcaagcgtg
     6781 cagacgcgct ccgcgtcccg ggccgcgatg gtgcccatgc tgaccttgtc ctggttgtgc
     6841 gattccgtcg accgcgaaaa agaagcggcg ggcatcgtgg ctttgagcgc ctcggcggcc
     6901 agcgccgacg acgcgatcga catggccttg aagccgtgat gcagaccttg agaagcgccg
     6961 tttttccgca ccaggtcgcc gggcaagccg cggttgacgt tcggattgac gagcagcatg
     7021 atctggcggt cgcacatatc ggcggcggac gcgagcgccg ccttgaggcc gtccatggca
     7081 aaggcgatgt gcccgccgta gaaattgccg cccatcagca cctgccccgt ggcgggatca
     7141 aagatgggat tgtcgttgga cgaattggcc tcgatttcca cccacggaat gatccaggaa
     7201 agggcgtcgg caagcacgcc cagcgcctgc ggggcgcagc gcagcgagta gggatcctgc
     7261 agggtttcca gcgcgtcgtc ctcgagccgg cgcgcggaca ctttcgaagt gagcaggcgg
     7321 gcgatttccc gggcgacggc tgcctggccg ggaaaaggct tggcctctga gatcaccgga
     7381 tggtaatgat gggcgttgcc tttcatggca tgcaccgaaa gcgcggtggc gtaaaccacg
     7441 gcatccagaa tccgccggga ccgctcgatg cagaccgcgg cgatgcccgt catggtcgtc
     7501 gtgccgttca ccatcgaaag gggttccttg ggccgataga catagggctt caatccggct
     7561 tttttgatcg cctttgcggc cggcatcacg tcgccttcgt aaaacacgtc gcgccggccg
     7621 gccagcgccg ctccgatata ggacatcggc gtcagatcgc cggaagcgcc gaccgatccc
     7681 tcgcacggca cgacaggcgt gatgcctttg ttcaaaaagg cggccatctg ctccagcagg
     7741 ccgaccgatg cgccggagta gcctcgcgcc aggcagagaa tgcgcgcaag catcgcggct
     7801 cgcgtttcct cgatgccgat gggctcgccg gtgccgcacc cgtgaaattt cagaatattg
     7861 gggccgtttt tcatggccgc cgcaagactg atccggtttc cgcaggattt tccatacccc
     7921 gtgttcacgc cgtaaaccgc gacgccgttc tccagagacg tcatcagcgc cttctcggtc
     7981 gccgacatcc gcttcagaaa cgcgcgcgaa cggctgatcc tgacgggcag gccgtctatg
     8041 gcgacggcaa tgatttcctc aaaacggacc gggccgccgt cgatgacaag ggcggccttt
     8101 gcgccgattt tcttcatgat cgtttggtct ccttcacaaa ccctgctctt caagccctta
     8161 cccgcggctt gtaggtgccg tccgctttct ccttttccat gacttgcttg agcatcttga
     8221 acatcttgta attggccggc tcttcctcgt aaaaccggtc ccagcaatag tagtacattt
     8281 cctgcagctt ttcgggcgtc atcattttgg gctgaaaaac caccttgccg caggtgtagt
     8341 cgttccagtc acaggagagg attcgaccct cgccgtgcag atcgtcaaac gtgcgcgtgt
     8401 ggacaaaagg cgtgagaatc gtgaactccg ccatgtccag ctcgatttcc agcagaaagt
     8461 ccatccagcg cttaatgtaa tcttccgtgt ggtagtccag gcccagcaga atgctccctt
     8521 ccacggcgat gccgtgatcg tggtagcgct tgatccggtc gcggatgtag tcggaggagt
     8581 cgaaaatggt ctgatagaca aaccacgcgc ccgcctgcgc cgccagatcc agaacctcgt
     8641 cgtcgtcttc gatcggatgg cagcaccact tctttttgag cggaatcatt tccttgaaga
     8701 gaccgatttc ccagtcgcgg tcctgcgcca gcgagttgtc cacaatgaac aggcggttgt
     8761 tgtcgatgga ggccatttcc tccatgaccc ggtcgtaggg gcgggggcgg aagcttcggc
     8821 cgcccagaaa gcgcaccgcg cagggatagc agttgaagcg gcagccgcgg gaggcgtgaa
     8881 ccagatcgac catctggacg ccgcggtagt tgtacatctc tcgtttgagg atgccgcgcc
     8941 gtgccgttcc cacgttttca atcggcggga agttctgcat gtagttgtaa actttttgca
     9001 gcttgcccgc ctggaaatcg gcaaagacct gctccatgcg gccttcggct tcgcccaaaa
     9061 acaccgcgtc ggcgtgcttg gcgcattcct cagcgtgcag catgacggcg atgcccccgc
     9121 agatgacggg aatcccccgg gcgcgaaacg catccgcgat ttcccagccg cgcttcacct
     9181 gcgcggtcag catcatcgag acgccgacaa aatcggcatc cgcgccgagg tccaccggct
     9241 cgacattttc atcgacaaac gccacctccg cgtcggaagg caaagcggct gccatcacca
     9301 ccggcccgtg cggcggaaga atgaaccgcg tctggcggtc cagcttttcc catttcggat
     9361 agatcagcaa aaatttcatg cattcccctt tatcattcac atcggctgaa aaccaggcag
     9421 gcacaggcgc cgccaaagga tatgccggat aaaaccgtgt gcttcaggtg ggttttcact
     9481 tttttgcccg cgacaaaatt caacggtgcg gccggccgtt ccaggccgac cgtcaaaggc
     9541 aggcatcctc tttccacaga taacgccaga gcgcaagccc gaattccgcc tccggaaaaa
     9601 ctctctccca gagcgccctt gatggaggtc acccagggac ggacggcgcg ggatgcaaat
     9661 aatccttcgt aagcggccgc ctccacggca tccagaaccc tgcttccgtt ggccgccgcc
     9721 atcacggccg acacgtccgc aacgtcaatc cgcgcctgct tgagcgcccg cagaaacgcc
     9781 cggcaaaacc cttccggatc ccgcggccag gccgtgggcg gcgcgggcga ggaccccagg
     9841 ccggcgccgg tgatttcgca gcatattttt gctccgcgct cacgggcgca agccagggac
     9901 tccagacaga ggaggccgca cccttctccc gccacatacc cgtttcgccg ctcgtcaaag
     9961 ggatgggcgc cttccggccc cccgtcatcc ggcgaaagcc cgcggaattt cgacagggct
    10021 tcatagtaaa atttcgacag aatatccgca ccgccggcca gcatcacatc cgccgtcccg
    10081 cgccggattt ctgaggcggc atacgccacg gccgtctccg ccgacaccgc gaaatgcgtg
    10141 accgtgacat tgacgccgcg aaaccccagc tctatggagg cgtacccggc gggcgcgttc
    10201 atgacggtgt tgggcaccag gatcggattg gccgcccccg gcccctgcgc caggagcgcg
    10261 tcttcaaact gcacggtgac gtcggtcgcg ccaaaggcgg tcccgaagag aatgcccacg
    10321 cggtcgcggt tttcggccgt gacggcaatg cccgcgtcgg aaagcgccag gcgcgccgat
    10381 gcggtcgcaa gcatcgacag ccggtccatc cgccgcagat tctttataga gataaaatca
    10441 cgcggctgga aatcgcgcac ttccgcgcca agatgcgacg ccgccgcacc ggtgtcaaag
    10501 gcctgcacgg catcgacggc gctctcgccc tgaaaaagcc tgcgggaaaa ttcctcccgg
    10561 cccgtgccga gcggcgttgc catccccagg ccggtgatca ccacccgccg gttgtccgga
    10621 aaatcgcgat gctcactcat ggattgcgcc ctgcccttca taccggccga aaatgaccgt
    10681 cgtgttgttt ccgccgaaag caaaggaatt ggatagaacg gcgcgcagct cggcccggcg
    10741 cgctccctgg gttacataat ccaggtcgca ttccgggtcg cgttcgcgcc agtggatcgt
    10801 cggcggaaca aatcttttgg acagagccag caggctgacg atcccttcca gggcgcctga
    10861 cgcgccgagg gtatgggcat gcatggattt ggtcgaactc accgggattt gataggcgcg
    10921 ggaaccgaac acctctttga tggctttggt ttccgttaaa tcattgaccg gcgtggccgt
    10981 gccgtgggcg ttgatgtaat cgatatcctc caccgtgagg ccgccgtccg ccagagcggc
    11041 ccgcatggag cgcgcggcgc cggacgcctt ttcatcgggc gacgtcatgt gaaaggaatc
    11101 gcaggtgacg ccgtagccca tcagctcccc gtaaattttc gcgccccgat tctgcgccgt
    11161 ctccagaggc tccagaacca gaacggccga tgcctcgccc agggaaagcc ccgaccggtt
    11221 cctgtcaaaa ggccggcaga cgtcctcgtc cacggatttg agggcgttga aggcggcgta
    11281 agtaagccgg cacatcggct ccacgccgcc cgccagcatg atgccggcgt ggccgttcaa
    11341 tatcaggtcc ctggcatagc ccagcgcagt ggcgcccgcc gaacaggccg tcataaaggt
    11401 ggccctgggg cccgaaaact ccaggctgga ggcgatgcgg tccgcggacg aggcgcaaaa
    11461 caaagaagac agttgagaat acttcgaacg ctccttccct tttcgaagca aatccgcgta
    11521 gaagctttcc gcttccagaa gcccccccgc gccgccccca atggctattc ccatgttttc
    11581 tttaatgttt tcctgtaagg ggtaaaggcc tgcatcagtc agagcctcga gggccgccgc
    11641 catggacaga gcatcggcgc gggatattct tttgagagaa aactcttccg gaatcgaccg
    11701 gcggggatca aagtctttga cctgcccccc cttatgcgtg cggaaatccg tggtgtcgaa
    11761 aagatcgatc tgagagatgc cgcagacgcc ttcccgaagc gaccgggcaa actcggaaac
    11821 gtttccgcca atggcgtttt ttgttcccat gcctgtaatg acaatacgtt ttttcactat
    11881 ccatgaatct ccttgttccc gctttcggca agggcatgct tggccttttc gagctttaaa
    11941 tggaacagtc tcaaaagtca aatgcatatt ccggcgccgc gcacggcttg aaaaatttgt
    12001 tttgacatca acccctgcat ctcctatgat tccccgcgca aaaaatttaa aatccgacgc
    12061 tgaaggagaa tgcaatgttc aagacgcgaa tgaccgagat gttcggcatc cggcatccga
    12121 ttatgctcgc cgggatgaac tggatcacgg agcccaaact ggtttcggcc gtctgcaacg
    12181 cgggcgggct gggcattctg gccatcgcgc gctgcaaccc caaagaaaca cgggaaaata
    12241 tcaagcaaat acaagatctt acggataagc cgttcggcgt caatcagatt ctcgtgggac
    12301 cgggcgcaaa ggaaaacatc gccgtcgcca tcgaagaaaa agtgccgatc ataaactatt
    12361 cgctggggaa accctggttt gtcaacgacg tgcacgccta cggcgggaaa gtcgtgggga
    12421 cgatcgccat tgcccggcat gcggccaagg ccgtgcagct cggctgcgac gcgctggtca
    12481 tcaccggcca tgaagcggcg gcgcacggcg cggcggcaac ctccatggtt ttgattccga
    12541 ttgttgcatc catggtaaag gtgccgctca tcgccgcggg cggattttac gacggccggg
    12601 gacttgcggc tgcgctcgcg ctgggcgcgg acggcatttc catgggcagc cgcttcatgg
    12661 tggtcaagga aagcatggtg cacgaaaact tcaagcgcct gtgcatggag gcgaccgaac
    12721 aggatacgct ctacgataat gtttttgacg gcatggacgg ccgcgtgctg aagacgaagg
    12781 aagccctggc gatgatgaag agaggcttcc cgattgttga agcctttaaa ggcgccctgc
    12841 tggtcaaacg gctgatgaat ctgtcgtttg ccaaattcct gggcttgagc cttgaaatgc
    12901 tgcgcgccga ggaaggccac cccctgatgg ttctggcgcg ccaggcggtg ggcaacatga
    12961 agcatctcaa agccctgaac gacggggacg tggaaggcgg cattttgttt gccgggcaat
    13021 gctgcggcgg catcaccgac aacccgacca cgaaggagct gatcgaccgg atcatccgcg
    13081 aggccgaaga ggcgcaggcg agattaaaca gttgccggac ataaagagag aatcaaccgg
    13141 cccgcggccg aagaacatag aaagaacaaa gacttcaaaa gcttatgcaa ctgacactga
    13201 tccgccacgg cgaaaccgtc tggaaccggg agaagcgcgt gcagggtctc accgatgtcg
    13261 aactgagcga aaccggcatc gaccaggccc gaaggctcgc gttgtcgctc agagacttcc
    13321 cgatcgacgt catctactcc agcccgctca agagggcctt tcagacggcc gaactcatca
    13381 accggtatca ccggaaagcc atcaacgtcg aacccggcct gatggaaatg gaccaggggg
    13441 actttgaagg tctgacgttt caggagctgc tggcccgcga gcaagatttt atccgcagtt
    13501 ggattgccga tccggaatcg gtgagaatgc cggggggaga atcgctggcc gaactgcaga
    13561 gccgcgcctg gccgcccgtt gaaaagctca tggccgaagg ccggaacgcc ctgatcgttt
    13621 cgcacaactt cacgatcgcg acgattttgt gccgtttgag aaacattcgg ctttccgaat
    13681 tccgcagcac ctgcgtggac aacgcctccc gaacgcacat ccgcattgag gaaggagcag
    13741 cgcgcatcga agccatcaat gacgtccgcc atttaaaaac agagacggat gtctagaacc
    13801 gtttcagggt gacttgctga tgaactcgta aaaagtctaa ataatggcaa attcaggcgg
    13861 cttcgtaaaa agctccgcag gcaaggcgcg caaatcatga ggaatgaggc gtacttcagc
    13921 gtacgccgcg atgacgaagg atgcagcgca acgcagcatc cgggcttttt acgaggccgt
    13981 cacttgttga tcttcgcgct gatccgctcc tgatactcca ggatttcatg gcgcaggatg
    14041 tccaggtcgg cgatccggtc gtcgagtctc tttaagtgat tattcagcag ttccagaaga
    14101 cgcctcagca gtttctcgtt ggatttttcg tagctccaca tggattcgag ttcctgcatt
    14161 tcggaaagcg tcatgcccat gatcttca
//
LOCUS       DAOSIM010000029        14035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_4440_length_14035_cov_8.118455, whole genome shotgun sequence.
ACCESSION   DAOSIM010000029 DAOSIM010000000
VERSION     DAOSIM010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 14035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14035
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4440_length_14035_cov_8.118455"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PLR47_02515"
     CDS             complement(<1..799)
                     /locus_tag="PLR47_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG11840.1"
                     /translation="MAEVNEQDFYDKVFPVPDRVREKSYIKSRAEYEKLYKESIENPN
                     AFWAKMATERLSWFKPFDKNKVSDWSFDAKNVYSKWFEGGKLNVCYNCLDRHLETKKN
                     KAAIIFEGNDPNDWKVYTYYDMYREVNKFANVLKSLGVKKGDRVTIFLPMIAELSIVM
                     LACARIGAVHAIVFGGFSAEALRDRINNSGSQVVITCDGTYRGAKAVPQKDTCDKALA
                     QCPGVKTLVVVKRTGTAVTMKEGRDVWYEELMAKADRYCKPEEMDAED"
     gene            complement(1221..2045)
                     /locus_tag="PLR47_02520"
     CDS             complement(1221..2045)
                     /locus_tag="PLR47_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG11841.1"
                     /translation="MALFSIDRDKCRSDGICAAQCPAQIIVQADRQSFPSLLNGAEEF
                     CIRCGHCVAVCPHGAFSLSGMTPDECPPVDPGLLPGADALKHLLLARRSIRRYKKTPV
                     SRKVLAELLDVARCAPTGSNKQQVHWIVYSQQEKVRRLAGMVVDFMKIMLPAAADEAA
                     LRRFRRVVDAWDEGQDRITRDAPHLIIAHSPSDLSFPAADCAIALTYLELYACARGLG
                     TCWAGYFTAAANLHEPLIRELNLPAGRQCCGAVMLGYPLCRYYRIPARNEAQIHWR"
     gene            2210..4591
                     /locus_tag="PLR47_02525"
     CDS             2210..4591
                     /locus_tag="PLR47_02525"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG11842.1"
                     /translation="MAKLTLDEWIARLKKNKSFNAGAAAVVDIPARPGDWEDYPSWVS
                     PNLRDVLVKRGMERLYRHQAQAVRFIRGGQDVVLVTPTASGKTLCYNLPVLQSILDEP
                     ETRALYLFPTKALAQDQMHEAHSLISDLQADIKTFTYDGDTPDDARRAIRRQGHIVVT
                     NPDMLHAGILPHHTKWQKLFVNLKYVVIDELHIYRGIFGSHFANVIRRLIRICRFYGA
                     NPVFICCSATVANPREHAEKILERDVALLDKSGAPAAAKTFVLFNPPIVNRELGIRQS
                     AMTPARKIASDLIGNHIQTIVFATSRLNVEVLTKYLKDKFVKSKPLDDHFVTGYRGGY
                     LPNLRRDIENGLRTREVMGVISTNALELGIDIGNLEACIMAGYPGSIASTWQQAGRAG
                     RRTGRSLAILIARSNPLDQFIMENPDYFFSLSPEHCRINPDNLLILLHHIKSAAFELP
                     FEKGEKFGGENLEEFLQYLEEKGVLHRTDDRWHWAAESYPADEVSLRAINPENVVVVD
                     ATDAGNHKVIAEVDWDSAFTAVHDEAIYMLASEQYHVDKLDLERKKAYVRKVDSDYYT
                     DAMTYTNVRVIDSFDNFRTGGTIVEHGEVQVVRKVVGFKKIKFYTSENLGYGDVNLPE
                     KDMHTTSYWFTIPRDRLRGLDFTPAEIIDGLSALAYTLQHLSAMFLMADTHDIDRSLG
                     DKSGEWFVSQGKTGRVITSFSGGRPQAINSSDAARFDEFDPTIFIFDSYPGGIGFSEL
                     LYREHANLLAAAKSLIEKCPCAFGCPSCVGPTLEVGKTAKEIVPKIIDLVLNQ"
     gene            4588..5916
                     /locus_tag="PLR47_02530"
     CDS             4588..5916
                     /locus_tag="PLR47_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOG11843.1"
                     /translation="MKSIDKLKRLTGEDKAPKKTEPVQPDSASSSERQSQLADLRGRI
                     ENVVTRGALRTRAAQEEARARGNRALCELLEGEEIENRHGRFFLAGGFLSGVSRHGHR
                     SICEAFNFDMTAAGMLANNPVLGAYHPSDALFLDTETTGLSGGTGTMAFLIGLGWFEE
                     GRFHIRQILARDFGEEPAALACLQEIAAQKKFLVTFNGKAFDVNLLATRFILNRMQSS
                     LHELPHLDLLHPSRRLLGHRLENCRLATLEADILGVEREGDIPGWEIPQRYFDWLRRR
                     DPRLLADIFEHNRLDVISMATLTAHLVDLLNAQALQQHAHPDDYLAAARLLLKKAGTA
                     GVYKILEILGENACAELSLSSKKNLANLCKRTGRMDEAVRIWRRMLDCDPNDFYAVTE
                     LAKHLEHRARDYGQAKALIEKAMAGDNFFSEEEKDSLEHRLNRLNARKTL"
     gene            complement(6048..7418)
                     /locus_tag="PLR47_02535"
     CDS             complement(6048..7418)
                     /locus_tag="PLR47_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG11844.1"
                     /translation="MKDTLEILIVDDDAVHRRAVRLNMAGWGYSVTEADDGEEAVRQA
                     RLRSFDLILMDIRMTRMSGLEALEQIKKIQPAVPVIIMTAYASVETAVSAMKMGADDY
                     LTKPLNYDELQIKIARTAERRQLKKENEYLKERLGEKFDRRNLIGRSEPMIRLLEMVE
                     QVAATQATVLIAGESGTGKEMIANAIHFNSPRREAPFVKINCAALTETLLESELFGHE
                     KGAFTGADRRREGKFVQADSGSIFLDEVSEMSPAMQVKLLRVLQERELTRVGGQDVVK
                     VDVRLIAASNKDLKKEIREGRFREDLFYRLNVVALALPPLRERAEDVPLLAQHFLKLF
                     ARRNARTISGFTPRAMQKLAAYAWPGNVRELMNAVERAVVLSRSDTVDEDELVFPMAD
                     QSLSPDAPRSAAPTAGLPLEEVEKRSILEAVKAAGGNKSEAARRLGITRKTLRKKLTK
                     YEDRHS"
     gene            complement(7415..9103)
                     /locus_tag="PLR47_02540"
     CDS             complement(7415..9103)
                     /locus_tag="PLR47_02540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG11845.1"
                     /translation="MPEKQKRKVRFDISVWVVISAVAVLAVILGMMMFMQFQHAQNQA
                     RLLLIDKGTTLIHSFESGLRLLPEQMEEDFYLQKLLMETAQQPDVDYLMVTDDEGHIL
                     ADSDPSMLGLPYGLDLDVKKIAQSRKIHWRQTANPEGAGTFEVYRGLSPMPGNAGKSR
                     RIVYAGFNMEKIEKASREDALRTIVTALVLVLIGSLAIVSLFLVQAYRLARVSLSRVT
                     VFSEALVKNMPIALLAVDREGKVAACNEQAGGLLAGAPAEAVGKEASLVLPGPFMRLL
                     EDVRGRTGPVEEDIRLVSENGEQTWEVVAAVFADDEAHEGRILLARNVTGVRQMEKEV
                     ARSRHLNAIASLAAGVAHEIRNPLSSIKGFAVYFKKRLAGNREDEETADIMIAETERL
                     NRVISQLIEFARPLVLKREPADLEDLVRQSVRLVKAEAEQSAVAVEIQAEEGLSRAEV
                     DPDKVRQVLLNIFLNALAAMPKGGRLSVALNRRDDDVEIAVSDTGEGIRQENLPRIYD
                     PYYTSKPAGTGLGLAVAQKIMDAHGGAIEVESREGAGTRVALRFPFMEKEGMGQ"
     gene            9311..9823
                     /locus_tag="PLR47_02545"
     CDS             9311..9823
                     /locus_tag="PLR47_02545"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOG11846.1"
                     /translation="MKKITLTLTAVLAGLLLTTSAFAWGPGPGKGRGYGPCGNAGLEK
                     LNLTDDQKAKIEALQDANDKATRPVREKIFDKSVELRRLWLQANPDKGKITTAQKELR
                     TLRNELEDKATSLRFEMNKVLTPEQKDKLANSRWGGKTGFGPRGGMRGDCGFGAGQGP
                     GPCLGMGMYQ"
     gene            10177..>11076
                     /locus_tag="PLR47_02550"
     CDS             10177..>11076
                     /locus_tag="PLR47_02550"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOG11847.1"
                     /translation="MKRDKNHVYIFDTSLRDGEQSPGNSMNTEEKLLLARQLEKLGVD
                     IIEAGFPIASEGDFDAVRQIAREIRKSQVAGLARANKQDIDRAWQAIKGAAHPRIHTF
                     ISSSDIHLKYQLKKSRAQVLREAAAAVKLAKSYTPNVEFSPMDATRSDRNYLAEMVSA
                     VIDAGAVTVNIPDTVGYAVPDEFGSLIAFLFDRVKNMGDTVISVHCHNDLGLAVANSL
                     AAIRAGARQVECTINGIGERAGNTAMEEIVMALATRRDLFGLTTQIKTDQIYKTSRLL
                     TQITGIPVQPNKAIVGANAFAHES"
     assembly_gap    11077..11086
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11087..11701
                     /locus_tag="PLR47_02555"
     CDS             <11087..11701
                     /locus_tag="PLR47_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOG11848.1"
                     /translation="TYEIMTPQSVGISDSHIVLGKHSGRAAISQHLKKMGYILNEDQL
                     NRVAAKVKDLADVKKDIYDEDMEAILYEEIYRGEEKYNLIYLNVVSGNVAIPTATMEM
                     QVGKEVIRDAGFGNGPVDATFAAIRNITKTNYPLLKYAVNAITGGADAQGETMVQLKY
                     NGNSVIGRGAHPDVIVASAKAYINALNRLEFLKDNAKKVKLELS"
     gene            11769..13028
                     /gene="leuC"
                     /locus_tag="PLR47_02560"
     CDS             11769..13028
                     /gene="leuC"
                     /locus_tag="PLR47_02560"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOG11849.1"
                     /translation="MGYTITEKILMAHTDLKEIAPGRLINARVDIALGNDITAPIAIR
                     EFRAAGGKKVFDRDRVALVLDHFTPNKDINSAQQCKTVREFAVEHGITHFYEGGQVGV
                     EHALLPEKGIVLPGDLVIGADSHTCTYGALGAFATGVGSTDLAAVLLTGELWFKVPES
                     IKFVVYGKPQKWVSGKDLILHIIGRIGVDGALYSAMEFTGETIRALPMADRLSMANMA
                     IEAGAKNGIFPPDEITREYVKPRAKRPYAFYTSDPDAVYAQTIEIDASRLEPQVAFPH
                     LPSNVRAISQAGEVKIDQSLIGSCTNGRIEDLRVAAGILKGRKAAPHVRLIVVPATPF
                     IYRQALQEGLLEIFMDAGAVISPPSCGACLGGHLGILAEGEKSVATTNRNFVGRMGHP
                     RSEVYLAGPAVAAASAVLGRIASPEEL"
     gene            13048..13533
                     /locus_tag="PLR47_02565"
     CDS             13048..13533
                     /locus_tag="PLR47_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOG11850.1"
                     /translation="MILKGKIWKFGDNIDTDAIIPARYLNTSDPAELARHCMEDADPA
                     FMTRMTPGDIIVGGENFGCGSSREHAPIAIKAAGISCVVAKSFARIFYRNAFNMGLPI
                     FESRDLADAIAEGAKITLDSAQGSITVEGQNQTFSISPIPPFMEQLIADGGLMKHIAG
                     K"
     gene            13541..>14035
                     /locus_tag="PLR47_02570"
     CDS             13541..>14035
                     /locus_tag="PLR47_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOG11851.1"
                     /translation="MTKQNLKIAVFAGDGIGPEIIREALKVLKLVADRRGISLSLASG
                     LIGGSAYDACGTPFPDESLHLALQSDAVLLGAVGGPKWEGLDYSLRPERALLGLREKL
                     GLYANLRPVTVFDELADASPLKKDLVQGIDLLILRELTGDIYFGKPRGVTKDQSGRRT
                     GVNTM"
BASE COUNT         3084 a         4178 c         3910 g         2853 t
ORIGIN      
        1 gatcttccgc gtccatttct tcgggtttgc agtagcggtc cgctttggcc atcaattctt
       61 cataccagac gtcgcggcct tccttcatgg taacggcggt tccggtacgt ttcacaacca
      121 ccagggtttt cacgccgggg cactgagcca gggctttgtc gcaggtgtct ttctgtggaa
      181 cggctttggc gccgcggtag gttccgtcac aggtgatgac gacctgtgag ccggaattgt
      241 tgatgcggtc tcttaacgct tccgccgaaa aaccgccgaa aacgatggca tgaacggcgc
      301 cgatgcgggc gcaggccagc atgacgatgg acagctccgc gatcatgggc aggaagatgg
      361 tgacacggtc gccctttttg acgcccaggc ttttgagaac gttcgcgaac ttgttgactt
      421 cgcgatacat gtcgtaatac gtgtaaactt tccagtcgtt gggatcgttg ccttcaaaaa
      481 tgatcgcggc cttgttcttc ttggtttcca gatgacggtc caggcagttg tagcagacat
      541 tgagctttcc gccttcaaac catttggaat acacattttt ggcgtcaaac gaccagtccg
      601 aaaccttgtt cttgtcaaaa ggtttgaacc agctcagtct ttccgtcgcc attttcgccc
      661 agaaagcatt ggggttttcg atcgactctt tgtaaagttt ttcgtactcc gcacggctct
      721 tgatgtagga tttctccctt actctgtccg gtaccggaaa aactttgtcg taaaaatctt
      781 gttcgttgac ttcggccata ttattctcct taaaattatt tttatgcctt ccttttccgc
      841 caaaaaggca ttatttctct tttgcaaacc agggacccag tgtcatattc gatgctttaa
      901 gggggagaca cgggacgcca tcggtctttt caagaggggt acgccccgcg attcgggcct
      961 tgttcctgtt tatcagatgc gcgcttctga tccaccgacc gcaaaacctc ggggcgtatt
     1021 acatatggcg gatgcaatac atgtacccct tgaaaagcgc atactgagta cactgtttcg
     1081 ggggcgcttg gtatcagtct ccgtccgatt ctgttgaagg gatatgtcct acaggcgcgt
     1141 ccgtgtttgt ccgacaaagg acaggccgaa ggcgagaggc gatgggctat cggccatgaa
     1201 gtccgagcat cgagcgatca tcagcgccag tggatctgcg cctcattgcg cgcgggaata
     1261 cggtaatagc ggcacaacgg gtatccgagc atcacggcgc cgcagcactg gcggcccgcc
     1321 ggaagattca gctcccgaat cagcggctcg tgcagattcg ccgctgccgt gaaatacccg
     1381 gcccagcagg tgcccagtcc ccgggcgcag gcgtaaagct ccagataggt gagcgcgatc
     1441 gcgcagtcgg ccgcgggaaa ggaaagatcg gaggggctgt gcgcgatgat caggtgcggg
     1501 gcatcccgcg taatgcggtc ttgtccctcg tcccaggcgt ccacgacccg ccggaagcgg
     1561 cggagggccg cctcgtccgc ggccgcggga agcatgatct tcatgaaatc gaccaccatt
     1621 ccggccagcc tgcgcacttt ctcctgctgt gagtaaacaa tccagtgaac ctgctgcttg
     1681 ttgcttcccg tgggcgcaca gcgggcgacg tccagaagct ccgccagcac cttccgggaa
     1741 acgggcgtct ttttgtaccg tcgaatggag cgcctcgcca gaagcagatg tttgagcgca
     1801 tccgcgccgg gaagcaggcc cggatcgacc ggcggacatt cgtcaggggt catgccggaa
     1861 agggaaaaag ccccgtgagg acaaaccgcc acgcagtggc cgcagcggat gcagaattct
     1921 tccgcgccat tgagcaaaga cggaaaagac tgccggtccg cctgaacgat gatttgggcg
     1981 gggcattggg ccgcgcagat tccgtcgctc ctgcatttgt cccggtcgat gctgaaaagc
     2041 gccatggctt ctcccctttt cttcaaaaga ttgattttgt tccgattcgt cccttaccgg
     2101 gcagataaat cattcacctg tttattgcaa tgaatttttg ctaattctct gttgtgatat
     2161 ttcgtttttt tatctatcat gcccgccgga aagaacaagg aaaaatcgca tggccaaatt
     2221 gaccctggac gagtggatcg cccgtctgaa aaaaaacaaa agcttcaacg ccggcgcggc
     2281 ggcggtggtg gatatcccgg cccggccggg ggattgggaa gattacccgt cctgggtgag
     2341 cccgaatctt cgcgatgttc tggtcaagcg cggaatggag aggctctaca ggcatcaggc
     2401 ccaggcggtg cgctttatcc gcggcgggca ggatgtcgtg ctggttacgc ccacggccag
     2461 cggcaaaacg ctttgctaca acctgccggt gctgcagagc attctggacg aaccggaaac
     2521 ccgggcgctt tacctgtttc ccaccaaggc gctggcgcag gaccagatgc acgaagcgca
     2581 cagcctgatt tcggatttgc aggccgacat caaaaccttt acctacgacg gcgacacgcc
     2641 cgatgacgcg cgccgggcca tccgccggca gggacatatc gtcgtcacca atcccgacat
     2701 gctgcacgcg ggcattttgc cgcaccacac caagtggcaa aagctgtttg tcaatctaaa
     2761 atacgtcgtc atcgacgagc tgcacatcta ccgcggcatt ttcggatcgc attttgccaa
     2821 tgtcatccgc aggctgatcc gcatctgccg cttttacggc gcgaatcccg tcttcatctg
     2881 ctgctcggcc acggtggcca atccccgcga acacgcggaa aaaattctgg aacgggacgt
     2941 cgcgcttctg gataaaagcg gcgcgcccgc ggcggccaaa acctttgtgc tgttcaaccc
     3001 gcccatcgtg aaccgcgagc tgggcatccg ccagagcgcc atgacgccgg cacgcaagat
     3061 cgcctccgac ttgatcggca accatattca gacgattgtc tttgccacca gccgccttaa
     3121 cgtcgaagtc ctgaccaagt atctcaaaga caaattcgtt aaaagcaagc ctctcgacga
     3181 ccactttgtc accggctacc gcggcgggta tctgccgaac ctgcggcgcg acattgaaaa
     3241 cggtctgcgc acgcgagaag taatgggcgt catcagcacc aatgcgcttg agctgggcat
     3301 tgacatcggc aacctggaag cctgcatcat ggccggctat cccggctcga tcgcgagcac
     3361 ctggcagcag gcgggccgcg cgggcaggcg aacgggacga tctctggcta ttttgatcgc
     3421 gcgaagcaac ccgctggatc agttcatcat ggaaaatccc gactacttct tttctctatc
     3481 gcccgagcat tgccgcatca atccggacaa cctgctgatc ctgcttcatc acatcaagag
     3541 cgcggccttc gaactgccgt ttgaaaaagg agaaaaattc ggcggcgaaa atctcgaaga
     3601 gtttttgcag tacctggagg aaaaaggcgt cctccaccgg acggacgacc gctggcactg
     3661 ggcggcggaa agctacccgg ccgacgaggt cagccttcgc gccatcaacc cggaaaatgt
     3721 cgtggtcgtc gatgcgaccg acgcgggcaa tcacaaagtc attgccgaag tggactggga
     3781 cagcgcgttt accgccgtcc atgatgaagc gatttacatg ctggcctccg agcagtacca
     3841 tgtggacaaa cttgatctgg agcgaaagaa ggcctacgtc cgcaaagtgg attcggacta
     3901 ctacaccgac gccatgacct ataccaatgt gcgcgtcatc gattcgttcg acaacttccg
     3961 cacgggcggg acaattgtcg agcacggcga ggtgcaggtg gtgcgcaagg tggtgggctt
     4021 caaaaagatc aaattctaca cctcggaaaa tctgggctat ggagacgtga atcttccgga
     4081 aaaggacatg cacaccacaa gctactggtt tacgattccg cgagaccggc tgcgggggct
     4141 tgatttcacg ccggcggaaa tcattgacgg actttcggcg ctggcctata cgctccagca
     4201 tctgtcggcc atgtttctga tggcggatac gcatgacatc gaccgatcgc tcggcgacaa
     4261 aagcggcgag tggtttgtga gccagggaaa aaccggccga gtcattactt cgttttccgg
     4321 cgggcggccg caggcgatta acagttccga cgccgctcgc tttgatgaat tcgacccgac
     4381 catcttcatc ttcgattcct atcccggcgg catcggcttt tcggaactgc tctaccgcga
     4441 gcatgccaat ctgctggccg ccgcgaaaag cctcattgaa aaatgtccct gcgcgtttgg
     4501 ctgcccgtcc tgcgtcggcc ccacgctgga agtgggcaaa acggccaagg aaattgtgcc
     4561 gaagattatt gatctggttt tgaaccaatg aaatctattg ataaattaaa acgcctgacc
     4621 ggcgaagata aggccccaaa gaagacggaa cccgtgcagc cggattcggc atcatccagc
     4681 gaaaggcagt cgcaactggc cgatttgcgc gggaggattg aaaatgtcgt cacccggggg
     4741 gccctgcgca ccagggcggc gcaggaggaa gcaagggcgc gcggcaaccg ggcgctttgc
     4801 gaactgctgg aaggcgagga gatcgaaaac cggcatggcc gtttttttct ggccggaggt
     4861 tttctttccg gcgtgagccg acacggccac cgcagcattt gcgaggcttt caattttgac
     4921 atgaccgcgg cgggcatgct ggccaacaat ccggtcctgg gcgcttatca cccttccgac
     4981 gcgctttttc tggacacgga aaccacgggg ctttccggcg gaacaggcac catggccttt
     5041 ctcatcggcc tgggctggtt tgaggaaggc cggtttcaca tccggcagat tctggcgcgc
     5101 gatttcggcg aggagccggc ggcgctggcc tgtcttcagg aaatcgccgc gcagaaaaaa
     5161 ttcctcgtga ccttcaacgg caaagccttt gacgtcaacc tgctcgccac acgcttcatt
     5221 ctgaaccgta tgcaaagcag ccttcacgaa ctgccccatc tggatctgct gcatccgtcg
     5281 cgcaggctgc tcgggcaccg tttggaaaac tgccgcctgg ccacgctgga ggcggacatc
     5341 ctgggcgttg agcgagaagg cgacattccc ggctgggaaa ttccgcagcg ctatttcgac
     5401 tggctgagaa ggcgcgatcc ccggctgctc gccgatattt ttgaacacaa ccggctggac
     5461 gtgatttcga tggcgacgct gaccgcgcat ctggtcgatc ttctgaatgc gcaggccctc
     5521 caacagcacg cgcatcccga cgactacctt gcggcggcga ggcttctgct caaaaaggcg
     5581 gggacggcgg gcgtttacaa aattctggag attctcgggg aaaacgcctg cgccgagctt
     5641 tccttatcct ccaaaaagaa cctggcgaat ctctgcaaac gcaccggccg catggacgaa
     5701 gccgtccgaa tctggcggcg aatgcttgac tgcgacccga acgattttta cgccgtcacc
     5761 gaacttgcca agcacctgga gcaccgcgcg agggattacg gacaggcaaa agcactcatc
     5821 gaaaaagcaa tggcgggaga taattttttt tccgaagagg agaaggattc tcttgaacat
     5881 cgactgaatc gtctgaacgc cagaaagact ctctgacacc tgcgcaagcc gctattttcc
     5941 cgcgtggaaa ctttttaaag accctcttaa aaaccgcact tcaccggcca acagatttat
     6001 tcaatggaag aatcgcagcg cttcccggcc aaaatggatc aaacttctca ggaatgcctg
     6061 tcttcatact tcgtaagctt cttgcgcagg gttttgcggg tgatgcccag acggcgcgcg
     6121 gcttcgcttt tgttgccgcc cgccgctttg accgcctcca aaatgcttct cttttccacc
     6181 tcctccagcg gcaggcccgc cgtcggcgct gccgagcggg gagcgtccgg ggaaaggctc
     6241 tggtccgcca tcggaaaaac cagttcgtct tcgtccaccg tatcggagcg ggacaggacc
     6301 acggcccgct ccacggcatt catcagctcg cggacgtttc cgggccaggc gtaggccgcg
     6361 agcttttgca ttgcccgggg tgtaaaaccg gagatggtcc tggcgttgcg ccgggcaaac
     6421 agcttcagaa aatgctgcgc cagaagcgga acgtcttcgg ccctctccct gagcggcggc
     6481 agggccagcg caacgacgtt cagccggtaa aacaaatctt cgcgaaaccg cccttcgcgg
     6541 atctctttct tcagatcctt gttggaagcg gcaatcaggc gaacatcgac cttgaccaca
     6601 tcctgcccgc ccacgcgcgt cagctcccgc tcctgcagaa cccgcaaaag cttcacctgc
     6661 atggcgggag acatttcgct cacctcgtcc agaaaaatgc tgccggagtc ggcctggacg
     6721 aactttcctt cccgccggcg gtccgcgccg gtgaacgcgc ccttttcatg tccgaacagc
     6781 tctgactcca gaagcgtttc ggtgagcgcc gcacagttga tcttcacaaa gggcgcttcc
     6841 cggcgggggc tgttgaaatg aatcgcgttg gcgatcattt ccttccccgt tcccgattct
     6901 ccggcaatca gcaccgtggc ctgcgtcgcg gcgacctgtt ccaccatttc cagaagccgg
     6961 atcatgggtt cgcttcggcc gatcagattc cggcggtcga acttctcgcc cagcctttcc
     7021 ttcagatatt cattctcttt tttcagttgg cggcgctccg ccgtccgcgc gattttgatt
     7081 tgcagctcgt catagttcag cggcttggtc agataatcgt cggcgcccat tttcatggcg
     7141 ctgacggcgg tttccaccga cgcgtaggcc gtcatgatga tcaccggaac ggccggctga
     7201 attttcttga tctgctcgag ggcttccagg cccgacattc ttgtcatgcg aatgtccatc
     7261 aggatcaggt caaacgacct caagcgagcc tgccggacgg cctcttcccc gtcgtccgcc
     7321 tccgtgacgg aataccccca gccggccatg ttgagcctga ccgctctgcg gtgaaccgcg
     7381 tcgtcatcga cgatcaaaat ctccagcgta tctttcattg tcccattcct tccttttcca
     7441 taaacggaaa gcgcagcgca acccgggtcc ctgctccttc ccggctttcc acctcaatcg
     7501 cgccgccgtg cgcgtccata atcttttgcg cgaccgcgag gcccaagccg gtcccggccg
     7561 gctttgaggt gtaatacgga tcataaatcc tgggcaggtt ctcctgccga atcccctctc
     7621 cggtgtcgga gaccgcaatt tcgacatcat cgtcacgccg gttcagcgca accgacagcc
     7681 ttccgccttt gggcatcgcc gccagcgcgt tgagaaaaat attgagcagc acctgcctga
     7741 ccttgtcggg atcaacctcg gcccgggaaa ggccctcctc cgcctggatt tccaccgcca
     7801 cggcgctttg ctccgcttca gccttcacca aacgaactga ctgccgcacc aggtcctcca
     7861 ggtctgcagg ctcccttttc aaaaccagcg gacgggcaaa ttcaatgagc tggctgatga
     7921 cgcggttgag cctttcggtc tccgcgatca tgatgtcggc cgtctcttcg tcttcccgat
     7981 tcccggccag cctttttttg aaataaacgg caaagccctt gatggagctc agcggattgc
     8041 ggatttcatg cgccacgccg gcggccagcg aagcgatggc gttcaggtgg cggctgcggg
     8101 caacctcctt ttccatctgc cggacgcccg tcacgttgcg ggcgagcagg attcttcctt
     8161 catgcgcctc atcgtcggca aaaaccgccg cgaccacctc ccaggtctgt tctccgtttt
     8221 cggaaaccag tcgaatgtcc tcttccaccg gaccggtccg tccgcgaacg tcttccagca
     8281 gacgcataaa aggcccgggc agaacgaggg aagcttcctt tcccaccgct tccgcgggtg
     8341 cgccggccag caggccgccg gcctgctcgt tgcaggcggc cactttcccc tcacggtcca
     8401 ccgccagaag cgcgatgggc atattcttga ccagggcttc ggaaaagacg gttacccgcg
     8461 atagagacac gcgcgccaga cgatacgcct gaaccagaaa gagggaaaca atggccagag
     8521 agccgatcag gacaagaacg agcgccgtca ctatcgtccg aagcgcgtct tcgcgggatg
     8581 ctttttcgat tttctccata ttgaatccgg cataaacaat ccggcggctc ttcccggcat
     8641 tccccggcat cggggaaagc ccccggtaaa cctcaaacgt gccggcgcct tcgggattgg
     8701 ccgtctgccg ccagtgaatt ttcctgcttt gcgcaatctt tttcacatcc agatccagtc
     8761 cgtagggcag gcccagcatg gacggatcgc tgtccgcgag aatatgacct tcgtcgtccg
     8821 tgaccatcag gtaatccaca tccggctgct gtgcggtttc catcagcagt ttctgaagat
     8881 agaaatcttc ttccatctgc tccggaagaa ggcgcagccc cgattcaaaa gaatggatca
     8941 gtgttgttcc cttatcgatc aggagcagac gggcctgatt ctgggcgtgc tgaaactgca
     9001 tgaacatcat catccctaaa ataacggcta aaaccgcaac cgcactgatg acaacccaga
     9061 cggaaatatc aaaccggact tttctcttct gtttctcagg catgacccct tctcccgcaa
     9121 atttctggat acaatgaacc cacctggata cccgcattgg atacgcatta tccgtccatt
     9181 gcgggcaaaa ggcaaagcaa tttgcgcgga ttttcataat gctcaatcat tgcaatacat
     9241 taaaagaatt ggcccatgtc ttgctgtaca caacggcaac agaacagaaa caaatgaaag
     9301 gagatgcacc atgaagaaaa tcacattaac gctaacagcc gttttggcgg gcttgctttt
     9361 aacgacttcg gcctttgcct ggggacccgg cccgggaaaa ggaagaggat acggaccctg
     9421 cgggaatgca ggcctggaaa aattaaacct gaccgatgac cagaaagcaa aaatcgaagc
     9481 attgcaggac gcaaacgaca aagccacgag accagtccgg gaaaagattt ttgacaaatc
     9541 cgtggaattg cgaagactgt ggctgcaggc caatccggac aagggaaaaa tcaccacggc
     9601 tcaaaaggag cttcgaacgt tgagaaacga attggaggac aaggcaacct ctttaagatt
     9661 tgaaatgaac aaggttttga cgcctgaaca aaaggataaa ctggccaact cgcgatgggg
     9721 cggaaaaaca ggctttggcc cccgcggcgg aatgagaggc gactgcggat tcggcgccgg
     9781 ccagggtccc ggaccctgtt taggaatggg tatgtatcaa tagccacttt gcccacccct
     9841 ccttttgaaa ggggagcttc ggctcccctt tttttatgaa aaagaaaaaa gttttgacaa
     9901 agcccgcttt ttgcattaac aagcaaaccg aatttttatt ttgaaaagga atttcatgtt
     9961 cagcttcacg acgacaacga ttttggctgg cgctcttttg accctgcttc cgggcctcgc
    10021 cctgttgcgc ctgttggtca aggccgcctg cccgccgttt ttaggtcgtc gctgaacaaa
    10081 agcataacca gtcacctaaa aaggccgcgg gcggaacccg cggccttttt ttatttggca
    10141 gacagtcttt tattttgaaa gcgaaaggag caacgaatga agagggataa aaatcacgtc
    10201 tatatttttg acacgtcact tcgggacggg gaacagtcgc cgggcaacag catgaacacg
    10261 gaggaaaagc tgctgctggc caggcagctc gaaaaactcg gcgtcgatat catcgaggcg
    10321 gggtttccca tcgcctcgga gggagatttt gacgcggtcc ggcagatcgc ccgggaaatc
    10381 cggaagtctc aggtggccgg attggcgcgc gccaacaaac aggacatcga ccgggcctgg
    10441 caggccatca agggcgcggc gcatccgcgc attcacacgt tcatctcttc gtcggacatc
    10501 catctgaaat accagctcaa gaaaagccgc gcgcaggtgc tcagggaagc ggcggcggcc
    10561 gtgaagcttg cgaaatccta cacccccaac gtggaatttt cgccgatgga cgccacccgt
    10621 tcggacagga actatctggc ggaaatggtt tccgccgtga tcgacgcggg cgccgtcacc
    10681 gtcaacattc ccgacacggt cggctatgcc gtccccgacg agttcggaag cctcatcgcc
    10741 ttcctgttcg accgcgtgaa aaatatgggc gatacggtca tctccgtcca ctgccacaac
    10801 gacctggggc tggccgtggc caattcgctt gccgccatcc gcgcgggcgc gcgtcaggtg
    10861 gagtgcacga tcaacggcat cggggagagg gccggcaaca cggcgatgga ggaaatcgtc
    10921 atggcgctgg ccacgcgcag ggatttgttc ggccttacca cccaaatcaa aacagaccag
    10981 atttacaaaa cgtcacgcct gctgacgcag atcaccggca ttcccgtgca gccgaacaag
    11041 gccatcgtcg gcgccaacgc gttcgcgcac gaatccnnnn nnnnnnacct acgagatcat
    11101 gacgccccag tccgtcggga tcagcgacag ccatatcgtt ctgggcaagc actccggacg
    11161 cgcggccatt tcgcagcatc tgaaaaaaat gggctacatc ctgaatgagg atcagctcaa
    11221 ccgcgtcgcg gcaaaggtga aagacctggc cgacgtgaag aaagacatct acgatgagga
    11281 catggaggcg attctctatg aagaaatcta ccggggcgag gaaaagtaca acctgatcta
    11341 cctcaacgtg gtgagcggca atgtggccat tcccacggcg accatggaaa tgcaggtggg
    11401 caaagaggtg atccgcgacg cgggcttcgg caacgggccg gtggacgcaa ccttcgcggc
    11461 catccgcaac atcacgaaga ccaactaccc gcttttgaag tacgcggtca acgccattac
    11521 cggcggagcg gacgcacagg gcgagacgat ggtgcagctc aagtacaacg ggaattccgt
    11581 catcgggcgc ggcgcgcacc cggacgtgat tgtcgcctcg gccaaggcgt acatcaacgc
    11641 gctcaaccgg ctggaatttc tgaaagacaa cgccaaaaag gttaaacttg aattaagctg
    11701 aaaaatggga tagttaaaac aaccttttta actttttaaa aagcattttt tatcagggag
    11761 tccatcgcgt gggatacacg ataacggaaa aaattttaat ggcgcacacg gacctgaagg
    11821 aaattgcgcc cggccggctc atcaacgccc gcgtggacat tgcgctgggc aacgacatta
    11881 cggcgcccat cgccatccgc gaatttcgcg cggccggcgg caagaaggtt tttgaccggg
    11941 accgcgtggc gctggtcctg gatcatttta cgccgaacaa agacatcaac tccgcccagc
    12001 agtgcaaaac cgtccgcgaa ttcgccgtcg agcacgggat cacgcacttt tacgagggcg
    12061 ggcaagtagg cgtcgaacac gcgctgctgc ccgaaaaagg catcgtgctt cccggcgacc
    12121 tggtcatcgg cgcggacagc cacacctgca cctacggagc gctgggcgct tttgccaccg
    12181 gcgtgggcag cacggatctg gccgcggtcc tgctcacggg cgagctgtgg ttcaaggtcc
    12241 cggagtcgat caaattcgtc gtttacggca aaccgcaaaa atgggtttcc ggaaaagacc
    12301 tgattcttca catcatcgga cgcatcggcg tggacggcgc tctctattcc gcgatggagt
    12361 tcacgggcga aaccattcgc gctctgccca tggccgaccg gctgtccatg gccaacatgg
    12421 ccatcgaggc cggcgcgaaa aacggcatct ttccgccgga cgagattacc cgggaatacg
    12481 tcaagccgcg cgccaaaagg ccttacgcct tttacacatc cgacccggac gccgtttacg
    12541 cgcagacgat tgaaatcgac gcctcccgac tggagcctca ggtcgccttt ccgcatctgc
    12601 cttccaacgt cagggccatc agccaggcgg gcgaagtgaa gatcgatcag tccctgatcg
    12661 ggagctgcac caacggccgg attgaagacc tgcgcgttgc cgccgggatt ttgaaaggcc
    12721 gcaaggcagc ccctcatgtc cgcctcatcg tggttccggc cacgccgttt atttacaggc
    12781 aggcccttca ggaagggctt ctggaaatct tcatggacgc gggggcggtc atcagcccgc
    12841 cgtcctgcgg cgcatgcctc ggggggcatc tgggcattct ggcggaaggg gaaaaatccg
    12901 tcgccacgac caaccgcaat ttcgtggggc gcatgggcca tcccagaagc gaagtttatc
    12961 tggccggccc cgccgtggcc gcggcttccg ccgttctggg ccgcatcgct tcaccggaag
    13021 agttataaaa gcaaagggga cggcatcatg atcttgaaag gaaaaatctg gaaattcggg
    13081 gacaacatcg acacagacgc gatcattccc gcgcgctatc tcaacacgtc ggacccggct
    13141 gaactggccc ggcactgcat ggaagacgca gaccccgcct tcatgacccg aatgacgccg
    13201 ggcgacatca tcgtcggcgg agaaaacttc ggctgcggct cttcgcggga gcacgcgccc
    13261 atcgccatca aggcggccgg aatctcctgc gtcgtggcca aaagcttcgc gcgcatcttc
    13321 taccgcaacg ccttcaacat gggcctgccg atcttcgaat cccgcgatct ggcggacgcg
    13381 attgccgaag gcgcaaagat aacgctggac agcgcgcagg gctccatcac cgtggaaggc
    13441 cagaaccaaa cattttcgat cagcccgatt ccgcccttca tggaacagct catcgccgac
    13501 ggcggcctga tgaagcacat tgccggaaaa tgagaaaagc atgacgaaac aaaacctgaa
    13561 aatcgctgtt ttcgccgggg acggaatcgg tccggaaatt attcgggaag ccctgaaagt
    13621 cctgaagctt gtcgccgacc ggcgcggcat ctccctttcg cttgccagtg ggctcatcgg
    13681 cggcagcgcc tatgacgcct gcggaacgcc ctttcccgat gaaagcctcc atctagccct
    13741 gcaaagcgac gccgtgctgc tgggcgcggt gggcggcccg aaatgggaag gactggatta
    13801 cagcctccgg cccgaacggg cgcttctggg cctgcgcgaa aaactggggc tttacgccaa
    13861 cctgcggccc gtgacggtct ttgacgagct ggcggacgcc tcgccgctca aaaaggatct
    13921 ggtgcagggc atcgaccttc tgattttgcg ggagctgacc ggcgatatct atttcggcaa
    13981 gccgcgcggc gtgaccaaag accaaagcgg ccggcgcacc ggcgtcaaca ccatg
//
LOCUS       DAOSIM010000030        13854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_4509_length_13854_cov_6.564824, whole genome shotgun sequence.
ACCESSION   DAOSIM010000030 DAOSIM010000000
VERSION     DAOSIM010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 13854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13854
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4509_length_13854_cov_6.564824"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1549)
                     /locus_tag="PLR47_02575"
     CDS             complement(80..1549)
                     /locus_tag="PLR47_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOG11852.1"
                     /translation="MAKIKWNWIWGIGITVVFLGLLAHRVEFFREKPPESPEPQTIEI
                     ARPPESWMNIYQNRRKIGVIHRTFTALENGRFQTRENVAMQINTMGVVQALNLSTESE
                     LNPDMTFSSFHFEINSSLFRFAARGYASHDKLIVFAGLPHAQKKTVLPLKDLPHIGGN
                     IYEAAFRGSLEKGQTRSFSIFDPSTLGLRKVAVTRHADEIIPIMGKRVLTQKFCADFM
                     GAQNCAWLAKDGETLKETGMMGLSMEKVDAQKAREGIAAEGIADFAQIASIASNRILP
                     EPSTLTEIVIRTGGVRNLPLLLSGGRQKFSSGILTITKEEMPDTAPPDKDPPLFGQYL
                     QPSPLVQSDAPEIQKQAAKIISPKDSPPARLRKIVHWVYRTIEKKPVLSVPNALEVLK
                     NRAGDCNEHAVLTAALLRAAGVPAQMETGLVYLNGRFYYHAWNVAYVGRWVTADAVFD
                     QVPADVTHIRLARGEGGEQLDLLGVMGKITLEVLSFKHD"
     gene            1693..2136
                     /locus_tag="PLR47_02580"
     CDS             1693..2136
                     /locus_tag="PLR47_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HOG11853.1"
                     /translation="MKKRYLIPLGLLYLALSSCAGCLTEEPSVQLRRVSLHPRSLTEI
                     QLILELDVQNPNRFDLTIQSLQYKLLLNEREIGSGRLEKEILAPALSTTRIQAPVTAG
                     FKNWNESLRAVIADKDIPYRIEGEANVRTIFGSIAYPFSRGGKIE"
     gene            2133..2687
                     /locus_tag="PLR47_02585"
     CDS             2133..2687
                     /locus_tag="PLR47_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOG11854.1"
                     /translation="MSSREGKIRTANGSLWYGVRGGGGNRMPVLVLHGGPGFSSMDNG
                     LEPLWKDRPVFFYDQLGCGKSDRAPDKNFYSVENYVAELAEVRAALKLDDVCLMGFSW
                     GCALACSYLLDRKPFGVKGVMLCAPYLSSPAWDADQRRNIALLPKDIRGAIEKGEKTG
                     DFGEAYESAMMAYYDRLRPPDNTP"
     gene            2758..3255
                     /locus_tag="PLR47_02590"
     CDS             2758..3255
                     /locus_tag="PLR47_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11855.1"
                     /translation="MPSREIATTVFGRNALTAGCICHAAALLAGMTTSSGADVFFKMI
                     YPASWVLMTAGAYAEMRRRGGRPLNSWRFYAAATASVFPLLGPPLVLGLIYTFAQIGR
                     EGQGDLSGFMPALGRMRGNLLTVFALILLLFLFFAFIHSRHDPYFQKNRQHGKIQQLC
                     GAGIV"
     gene            3301..7410
                     /locus_tag="PLR47_02595"
     CDS             3301..7410
                     /locus_tag="PLR47_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOG11856.1"
                     /translation="MVSGRRFSRLSTIWFCFLSLWILFPVVVSAGDSPPSPCGTEKKA
                     EIFIPAGHSGSVNAVEWSPDGRFILSGSSDGSIKLWDAQSGRLIRTFPDGEGILYVHF
                     LPGGKSFFSVNEKGGVGIWNIDGGARTGGFSLSGEKEPMIIDPVSFDGSVVRAIVSSR
                     LYAAQLSEGKVLPAEQLPEGAPVKKYFSGYDFGHKNLVTADGKRMISAVPEDPSDTRF
                     KKLILWELSTGNILFTLDGHTDRIDAVALSLDESYAASGGRDKTIRVWDLKTGKAAAV
                     FAGHQKEIESLAFSPDRKYVLSGSRDHTLRLWDIEAGKEVRAFAHRARVTLARFSPDG
                     RFAVSGDADGDIRIWDARTGEEVRALKSQADSGYVLVMSPDGRHVLSGTPDGKIRLWD
                     KTSGKLLETIQAHTERVAAVLFTPDGKYALSAGEDKTLKLWNPKGGTLLRTFAGHREK
                     IVRAAISPDGRRILSLDGSSEVRLWDLDSGAVQKTFPFPGRALSIGFADDSRHLLIAY
                     DDKVLDHRMIKVLTPEGREVKKYMSADFHFSEDGKYFLANEYWELDIAGGGILGRFGQ
                     SGAFLSLSVCAEEGIVLTKSRYDTDIVLWDVRTGLEIRRFSAKHGLLTYDGRKVVAPS
                     GAVLQAFDRASGGPLPPFTGKSSAQISSLALSPDGSVAVTGDVAGEVQLWDIGAGTLK
                     KSLPAMEKEMILATAVSSDRRFAAALARSGVIRIWNLSDGQKVSEVKTDYKPIYADEL
                     NAGDFVDYGNGILAFSPDGLRLACGSGVWETATGKSLLAFAQPNVPYPWVAFSPDGRF
                     LLSRNMIWDASSGQLERVLECGKNAAKSYYSLNGRLILCADLEGGFYTIDAATGRLTR
                     KFADFVPPGPFDVSRDKKMMVAVDKDQNEMTVWDLTSGRKNASIGVEQEVLSVSLTSD
                     RRRAILRHGISISEYDLPAGKEIVQLVSFTDGGWIVMTPEGYYNASSRGDRHINVRVG
                     DSVYGIENYRESFYRPEVVKRALAGDSLKDLPKLTDEKEPPKVRIVDTPASVAADEVH
                     VRLNLDEQGGGIGDIRLYLNGTAVVLDSRSVSEPEKSQRAVFRAYPLKLARGKNVIHA
                     AALNGDNTMRSNKAAWEVVTASYAAAAGPSLAVLAIGVGEYKDPGLKLPFAAASAELV
                     AGALKGASEGIYEKPAVKTMTRPEETTMEAILREIQTHQTLRAEDLFVLYIAAYAVVE
                     DGEYFLLTSNVNSLSAEKLKTDAISLRKLMEALGNIPTAGKLILIDACNPRIQSADAQ
                     AAFQTRAMSEETALKIFGGTLGLTVLASSAPLSEAVAGYQGHSLFVHLVAEGLRGAAD
                     RDKTGSIRSTDLTAYVRVEMPAQSEKIFKHTRYPVVSISGQAFSIGKVK"
     gene            7535..10831
                     /locus_tag="PLR47_02600"
     CDS             7535..10831
                     /locus_tag="PLR47_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOG11857.1"
                     /translation="MTRKLVFVYCFLIIMVFTTEAFCAKPLVPAGSSQGPDIFVQKGH
                     STNVNTIDFSPDGKYLVSGSSDKNIKLWDVETGREIRTFVARDMVDFVAFSPDGQSLL
                     SLESKGGAALWNVKTGREIRRLGKKGKPLLAIAAAFSPDGTSLAVVGGQDEKVPGGDL
                     TFRNIQLLDLNTGRELMIFKGHSSSIHTIAFSPDGKYLASGSETPNLNKGKKDDTVRL
                     WEVKSGKEIHRFAGHTEGVSKVAFSPDGQFVASIGEDRNIFFWNVGSGKQAKALSGFP
                     AGSVKTNGTIAFSPDGRRFVAVSSFPPVMILWDMVTGQVEAGMTDDHKLDDAAVRFSP
                     DGRLIAVSATDKVSLWDAAKHRKIRSFGGDVQGINLAFFSADQEQVKIAGNEELAVFD
                     RRQGRLTGRRTIEPFDNWWAKGQNEWGNRRFFKIIDKGYQVIDSRTDRAIISDAADRY
                     PTAYDSPQQAVTFSPDGRYALLAEKDGGKVSLLDVRSRRSIELVNFPDHVSLSSSDGY
                     AAFSPDGSLVAAASYSHDEERGVLVIWNTATGKQSIRLRANQKREGFSQVAFTADSKQ
                     IVAAEGGNQGILGLWDLTSGREIRSFPGHPGFITSLALSGDGKFVVSGDWIGTVKLWN
                     LRQGGEVKTFKGHTDEIKSLAFSRDGNYILSASKDGTARQWDIASGKEIAQFVSFTNG
                     EWIVITPEGYFNASPGGAKHLNVRIGLNVYSIDNLFEKFFNPVYVASVLQGKKVEATA
                     DIRKGILSPPEVRITSPAAGSVLNTDTLAVTVAARDTGGGIDEIRLYHNGKVVGGDQR
                     AVKIVPRGGETVRQYSVSLVDGANTFRAVGFSRDRTESNPYELMVRLKAPSKEVSLYV
                     LAVGINKYKNPALNLNYAEPDAKSIAGFFSQRGGGLFRKVNIREIYSEQATRENIVSQ
                     LNELQNTNPQDAVLIYLAGHGESLNDKWYFIPHELTYPEREADVRTKGVSSDDLSGYI
                     KNIKAQKILLLVDACKSGAVLIAFRGFEDRKALSQLSRSTGVHVVAASTKDQFAAEVK
                     DLGHGVFTYTLLAGLGGKAAGPGEPVTVRKLMVYVEEQLPEITKKYKQEAQYPVVDSR
                     GMDFPLVLGR"
     gene            10850..11710
                     /locus_tag="PLR47_02605"
     CDS             10850..11710
                     /locus_tag="PLR47_02605"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HOG11858.1"
                     /translation="MRKILFLAAMIILLAAPVYAAQSTIKIAEGSACMGDDKSRKQTE
                     TAALADAKRNAVESVKTYVSSATEVKDFELQKDLVSAYANATVEVLETLEKGWYKDPG
                     QGDCYRIKLKAEVIPDEKAMKKAGAKAADAALDDPSLPLTVKVWTDKTSYAAGEKIRI
                     YLKGNKPFFARVIYRDAVKNNLQLLPNPYRRDNYFQGGVIYEIPSGKDQFELEVNPPF
                     GEENIIVYASVSELGDLKLKDEGSIFAVQTRSKDIGVKTRSVKIKAASDDAGLAAEFS
                     EVKAVVKTRK"
     gene            11749..13542
                     /locus_tag="PLR47_02610"
     CDS             11749..13542
                     /locus_tag="PLR47_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG11859.1"
                     /translation="MKKAWVLFLCWLILLGSPAVVQDAKAQDSSPLKVVLIFDMGGRG
                     DGGFNDSAYSGMEKAVAELGVKAVYVEHKRNLELDYAVSGAAASDAEMIIGVGFAFSE
                     KFDHLAGRHPDKKFVVVDYSANYDERGNLISPPPNLAGLTFREEEGSFLVGAIAALKS
                     RSGKIGFIGGMDSPIIRKFQAGYEAGAKAVRPGIRIYSRYAGITGQAFRDPRKGYRIA
                     VSMYKNGADVIYHAAGETGAGLFQAARKMNRLAIGADIDQSAQAPGRVLTSMLKNIDG
                     AVFEAVQSCAHGNFSGGLKTLGLKERGVGFVYNNQNKKLIPESIHQQVQALRAKIVSG
                     ELAVPEASGHRTALSRQELRDLLSRLQSEIQTTLNRLDNDLRHGAKVLAGKDLRGDFA
                     RGILQGLYRANPYIIDCETVSDSGIMLAVEPPAHRSSEGADISRQSHMVRLFKTHTPV
                     LSGSFQSVEGPRAVVIHHPVFLKDRKFSGSVSALFAPEYLLSSMIGPIAAYMPVSIFL
                     MQTDGLFIYDVDAKQIGLNIFTDPLYQPFPELIALCRRMADTREGSGVYRFYRKVGEA
                     PIFKTVEWRTVELHGTPWRLAAAFAGDGVER"
     gene            complement(13539..>13854)
                     /locus_tag="PLR47_02615"
     CDS             complement(13539..>13854)
                     /locus_tag="PLR47_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11860.1"
                     /translation="MPGSDGKAGMAAVIRDENEPFDLARLAAHLKASLPKYAVPIFIR
                     FVPDFEWTATHKIKKTNLKNEGYDPAQVREELYALLPGSETYVPVSDEIYRNVTAGTY
                     KF"
BASE COUNT         3040 a         3662 c         4160 g         2992 t
ORIGIN      
        1 aatttctccg gcggccacct ccaggttcag gcggttgacg gccagcgtcg ttttgaaatc
       61 cttggtcaga ttgatcagtt taatcatgtt tgaaagagag aacctccaga gttattttgc
      121 ccatgacgcc cagcaggtcc agctgctcgc ctccttcccc gcgtgccagc cgaatatgcg
      181 tgacgtcggc cggaacctgg tcgaaaacgg cgtcggccgt cacccaccgg cccacatagg
      241 ccacgttcca tgcgtggtaa taaaaacggc cgttcagata cacgaggccg gtttccatct
      301 gagcgggcac gcccgcggcg cgaagcagcg cggcggtgag caccgcatgc tcgttgcagt
      361 cgccggccct gtttttcaaa acctccaggg cgttggggac ggacaggacg ggctttttct
      421 cgatggtgcg gtaaacccag tgcacgattt tcctgagcct cgccggcggg ctgtccttcg
      481 gggaaataat ttttgccgcc tgtttttgaa tttccggagc atcggactga accagcggcg
      541 acggttgaag atattgccca aagagcggag gatccttgtc cggcggggcc gtgtcgggca
      601 tttcctcttt agtgatcgtt aaaatgccgg aagaaaattt ctgcctgccg ccggaaagca
      661 gcaggggcag gttgcggacg ccgcccgtcc gaatgacgat ttccgtcagt gtggagggct
      721 cgggaagaat ccggttcgag gcgatcgagg cgatctgggc aaagtcggca atgccttccg
      781 ccgcaatgcc ttcacgcgcc ttttgggcat ccaccttttc catcgaaagg cccatcatgc
      841 ctgtttcctt tagggtttcc ccgtctttgg ccagccaggc gcagttttgg gcgcccatga
      901 agtcggcgca gaatttctgc gtcagaacgc gctttcccat gatcggaatg atttcatcgg
      961 cgtggcgcgt aaccgcaact tttctaaggc cgagcgtcga aggatcaaaa atgctgaagc
     1021 tgcgggtttg gcctttctcc aggctgccgc gaaaagccgc ttcatagatg tttccgccga
     1081 tatggggaag gtctttcagg ggcaggacgg ttttcttttg ggcgtgggga agccccgcga
     1141 aaacgatgag cttgtcatgg cttgcgtagc cccgggcggc aaaacgaaaa agactggagt
     1201 tgatctcaaa atggaaagag gaaaaggtca tgtcgggatt gagctccgat tcggtggaca
     1261 gattcagcgc ctgcacgacg cccatggtgt tgatctgcat ggcgacgttt tcgcgggtct
     1321 ggaagcggcc gttttccagc gcggtgaagg tgcggtggat tacgccgatt tttctcctgt
     1381 tttggtaaat attcatccag gattcgggtg ggcgggcaat ttcaatggtt tgcggctcgg
     1441 gagactcagg cggtttttcc cgaaagaact ccacgcgatg cgccagcagg ccgagaaaca
     1501 ccaccgtgat gccgatgccc cagatccaat tccatttgat ttttgccatg cgcgccaaac
     1561 tttcccagcc gtttcaaccg tcgaaattct gcaagagtat atcgacagga cgcggccggg
     1621 tcaactacca gtattggacc ggtattggac aatcccattg acagggaaag ccatccttcg
     1681 tatagtctcg ccatgaaaaa acgatatctg atcccccttg gattgctgta tctggcgctt
     1741 tcgtcctgcg cgggctgctt gaccgaagag ccttccgtgc agcttcgccg ggtttcgctt
     1801 cacccccgct ccctcacgga aatccaacta atcctcgaac tggacgtgca gaatccgaat
     1861 cgcttcgatc tgaccattca gtctcttcag tacaaacttt tgttgaacga gcgtgaaatc
     1921 ggaagcggac gcctggaaaa ggaaattctg gcgcccgcct tgtccacgac aaggattcag
     1981 gcgccggtca cggccggctt caaaaactgg aacgaaagcc ttcgggccgt gatcgccgac
     2041 aaggacattc cctatcgcat cgaaggggag gcgaacgtca ggacaatttt cggaagcatc
     2101 gcctatcctt tttccagggg agggaagatt gaatgagcag ccgcgaagga aagatccgca
     2161 cggccaacgg cagcctctgg tatggggttc gcggcggcgg cgggaacagg atgcccgttc
     2221 tcgtccttca cggcggcccc ggcttttcca gcatggataa cggtctcgag ccgctgtgga
     2281 aagaccgtcc ggtgtttttt tacgaccagt tgggctgcgg caaaagcgac cgggctccgg
     2341 acaaaaattt ttattcggtg gaaaattatg tcgccgaatt ggcggaagtg cgggccgcct
     2401 taaagcttga cgacgtttgc ctgatgggat tttcctgggg ctgcgcgctg gcctgctcct
     2461 acctgctgga tcgaaagccc ttcggcgtaa agggcgtgat gctgtgcgct ccgtatcttt
     2521 cttctccggc ctgggatgca gaccagcggc ggaatatcgc cctattgccg aaagacatta
     2581 ggggcgccat cgagaaaggc gagaaaacgg gcgacttcgg cgaggcgtac gaatcggcca
     2641 tgatggccta ctacgaccgg ttaaggcctc cggacaatac gccatgaaag tatttgccaa
     2701 acacaggggc gccaatatgt tgctggaaga aacgcctgat tcagcccgga ggcgataatg
     2761 ccgtcgcgcg aaatcgccac taccgttttt ggaagaaacg ctctgaccgc gggatgcatc
     2821 tgtcatgccg cggcgctgct tgccggcatg acgacctcgt ccggcgcgga tgtatttttc
     2881 aagatgattt acccggcttc ctgggttttg atgacggccg gggcttacgc cgaaatgcgg
     2941 cgccggggcg gccgtcctct gaactcctgg cggttctatg ccgcggcgac ggcgtcggtg
     3001 tttcctctgc tggggccgcc gttggttttg ggcctgatct acacgtttgc gcaaatcggc
     3061 agggaagggc agggcgattt gtccggcttc atgccggcct tggggcgaat gagggggaat
     3121 ctgctgacgg tttttgcgct gattctgctt cttttccttt tttttgcttt tattcacagc
     3181 agacacgatc cttactttca gaaaaatcgt cagcatggga aaattcaaca gctttgcggc
     3241 gcgggcatcg tatgaccttt ggcttatgat gtccacacgt tcaacatgaa gggagcatct
     3301 atggtttccg gcaggagatt ttctcgctta tcgacgatat ggttttgctt cctttcttta
     3361 tggattcttt tccctgttgt tgtatcggca ggggatagcc ccccatcacc ctgcggaacc
     3421 gaaaagaagg cggaaatctt cattccggca gggcattcgg gttccgtaaa tgcggtggaa
     3481 tggtcgcccg acggaaggtt cattctttcg ggaagcagcg acggcagcat caagctgtgg
     3541 gacgcccaaa gcggaaggct gatccgcacg tttccagacg gtgaaggaat cttgtacgtt
     3601 cattttctgc ccggcggcaa atcctttttt tccgtaaacg aaaaaggcgg cgtgggaata
     3661 tggaacattg acggcggcgc gcggaccgga ggattttcct taagcggtga aaaagaaccg
     3721 atgatcattg accccgtttc ttttgacggc tctgttgtcc gcgccatcgt ctcttcaagg
     3781 ctttacgccg cgcagctctc ggaggggaag gttctgcccg cggagcaact gcctgaaggg
     3841 gcgcccgtca aaaaatattt ttcgggatac gatttcggac acaaaaacct ggtgaccgcg
     3901 gacggcaaaa ggatgatttc cgccgtcccc gaagatccgt cggatacccg gttcaaaaaa
     3961 ctgatcctgt gggagctttc cacggggaat attcttttca cgctggacgg ccacacagac
     4021 cggatcgatg cggtagccct ctctctggac gaatcctatg ccgcgtcggg cgggcgggat
     4081 aaaaccatcc gcgtgtggga tttgaaaaca ggcaaggccg cggccgtgtt tgccggtcat
     4141 cagaaggaaa tcgaatcact cgccttttca cccgatcgga aatatgttct ttccggaagc
     4201 cgcgaccata ccctgaggct ctgggacatc gaagcgggaa aggaagttcg ggcctttgcc
     4261 catcgggcga gggttacgct tgcccgtttt tcgccggacg gacggtttgc cgtatccgga
     4321 gatgcggacg gcgacatacg gatctgggac gcgcgaaccg gtgaagaggt gagggcgctc
     4381 aaaagccaag ccgacagcgg ctatgtgctg gtgatgtcgc ccgacggccg acatgttttg
     4441 agcgggacgc cggacggaaa aatccgcctg tgggataaaa cttccgggaa actgctggaa
     4501 accattcagg cgcacacgga aagagttgcc gccgttctct tcaccccgga cggcaaatac
     4561 gctctttcgg ccggcgagga caaaaccctg aagttgtgga atccgaaagg cggcacgctg
     4621 ttaaggacgt ttgccggcca ccgggaaaaa atagtcaggg ccgcaatctc gccggacggc
     4681 cgaaggattt taagccttga cggaagcagc gaggtccggc tttgggattt ggattccggc
     4741 gctgttcaaa aaaccttccc gtttcccggc cgcgccctat ccatcggctt tgcggacgac
     4801 agccgccatc tgctgatcgc ctatgacgat aaagtgctgg accaccggat gatcaaagtc
     4861 ctgacgccgg aagggcgtga agtcaaaaaa tacatgagcg cggattttca tttttccgaa
     4921 gacgggaaat attttctggc caatgaatac tgggagctgg acattgccgg cggcggcatt
     4981 ctgggccgct tcgggcagag cggcgctttt ctatcccttt ccgtctgcgc ggaagaaggc
     5041 atcgtcctga cgaagagccg ttatgacaca gacatcgttc tgtgggatgt ccggaccggt
     5101 ctcgagattc ggcgcttctc cgccaagcac ggactgctga cgtacgacgg gagaaaagtc
     5161 gttgcgccgt cgggcgccgt cctgcaggcc tttgacaggg caagcggcgg gccgctgccg
     5221 ccttttacgg gcaagtcctc ggcccagatc agttcgctgg ctttgtcgcc ggacggaagc
     5281 gttgccgtaa cgggagatgt tgccggtgag gttcaacttt gggacatcgg cgcgggcaca
     5341 ctgaaaaaga gtctgccggc gatggagaag gaaatgattc tggccacggc ggtttcttcc
     5401 gatcggcgtt ttgcggcggc gcttgcccgt tccggcgtta tccggatctg gaatctgagc
     5461 gacggacaga aagtgtccga agtgaaaacg gattacaagc ccatctacgc cgacgagttg
     5521 aatgcgggcg atttcgtcga ttacggaaac ggaattctgg ccttctcccc cgacggcctt
     5581 cgccttgcct gcggatcggg cgtctgggaa accgcgacag ggaaaagcct gctggcgttt
     5641 gcccaaccca acgttcccta tccctgggtg gctttttccc ccgacggccg tttcctgctt
     5701 tcccggaata tgatctggga cgcctcctcc ggccagttgg agagagttct ggagtgcggg
     5761 aagaatgcgg caaaatctta ttattccttg aacggaaggc tcatcctctg tgccgatctc
     5821 gaaggaggat tttacacgat cgatgcggcg accggccgcc tgacgaggaa atttgccgat
     5881 tttgtgccgc ccggaccgtt tgacgtttcc cgcgacaaaa aaatgatggt cgctgtggac
     5941 aaagatcaga acgaaatgac ggtgtgggac ctgacgtccg gcaggaaaaa cgcttccatc
     6001 ggagtggaac aggaagtcct cagcgtatcg ctgacgtccg accggcgcag ggccatcctt
     6061 cggcacggga tttccatcag cgaatacgat ctgcccgcag gcaaagagat tgtgcagctt
     6121 gtcagtttca ccgacggcgg atggattgtt atgacgcccg aaggatatta caacgcctcg
     6181 tccagaggag accgccatat caacgtccgc gtcggagatt cggtttacgg catcgaaaac
     6241 taccgggaat ccttttaccg tcccgaagtg gtcaaaaggg cgctggccgg agattccctg
     6301 aaagatttgc cgaagttgac ggacgaaaaa gaaccgccga aagtgaggat tgtcgatacg
     6361 cccgcctccg tcgccgcgga tgaagttcac gtccggctga acctcgacga gcagggcggc
     6421 ggcatcgggg acatccgtct ttatctgaac gggaccgccg tggttctgga ttcccgttcg
     6481 gtttcggagc cggaaaagtc gcagcgggcc gtgttcaggg cctatcccct gaagcttgcc
     6541 cgcgggaaaa atgtcatcca cgccgcagca ttgaacggag acaacaccat gcggagcaac
     6601 aaggcggcat gggaagtagt gacagcctcc tatgccgcgg cggccgggcc gtcgcttgcc
     6661 gttttggcca tcggcgtcgg tgaatacaaa gatccggggc tcaagcttcc ctttgccgcg
     6721 gcgagcgcgg agcttgttgc gggcgcgctc aagggggcat cggaaggaat ctatgaaaag
     6781 ccggccgtca agaccatgac ccggccggaa gaaacaacga tggaggcgat ccttcgggag
     6841 attcaaacac atcagaccct gcgcgcggag gatctgtttg tcctgtatat tgccgcatat
     6901 gcggttgttg aggatgggga atattttctt ctgacatcca atgtgaattc gctgagtgcg
     6961 gaaaaactca aaacggacgc catctccctg cgaaagctga tggaggctct cggcaatatt
     7021 ccgacggccg gaaaactgat tctgattgat gcctgcaatc cccgcattca gagtgcggat
     7081 gcgcaagcgg ctttccagac gagggcaatg agtgaagaga cagctctgaa gatattcggc
     7141 ggaacgctcg ggttaaccgt tctggcttca tccgcgcctc tttcggaggc ggtggcgggc
     7201 tatcaggggc acagcctgtt cgtccacctg gtcgccgaag gcttgcgggg tgcggcagac
     7261 agagataaaa ccggcagcat ccgtagcacg gatctcaccg cctatgtgcg cgttgaaatg
     7321 ccggcacagt cggagaagat tttcaaacat acccggtatc cggtcgtttc catcagcgga
     7381 caggcgtttt ccatcgggaa agtgaagtag tggatggtgt atggtgcatg gtggatggaa
     7441 ggagggagac caaggagttg caaccaccta aaggtggcaa gtccacacct taggttggca
     7501 agattccttg ctccaggcgt atgaaagggt atttatgacc aggaagttag tttttgttta
     7561 ttgttttttg ataattatgg tttttacaac cgaagctttt tgcgccaaac cgcttgtccc
     7621 ggcgggaagt tcgcaggggc cggatatttt tgtgcagaag gggcattcca caaatgtcaa
     7681 caccatcgac ttttcgccgg atgggaagta tctcgtttcg ggaagctctg ataaaaacat
     7741 caagctgtgg gacgtggaaa cgggccgtga gattcggaca ttcgtcgccc gggacatggt
     7801 tgattttgtc gccttcagtc ccgacggcca atccctgctt tccctggaat ccaagggggg
     7861 cgccgcgtta tggaatgtaa agaccggtcg ggaaatcagg aggcttggca aaaaggggaa
     7921 gccgctcctc gcgattgcgg ctgctttttc tcccgacggg acgtctcttg ccgtggtggg
     7981 cggccaggac gagaaggtcc ccggcgggga tcttactttc cgcaacattc agctcctgga
     8041 tctcaatacc ggccgcgagt tgatgatctt caaagggcat tcctccagca ttcacacgat
     8101 cgccttttca ccggacggaa aatatctggc atcgggaagc gagacgccca atctgaacaa
     8161 gggtaaaaag gatgacaccg tcagactctg ggaggtaaag tcgggaaaag aaatacaccg
     8221 ctttgccgga cacaccgaag gagtcagcaa ggtcgcgttc tctcccgacg gtcagtttgt
     8281 agcgtccatc ggcgaggaca gaaatatttt tttctggaat gtcggcagcg gaaaacaggc
     8341 aaaggcgcta agcggctttc cggcaggcag tgttaaaacc aacggcacga tcgccttttc
     8401 tcccgacggg cggcgctttg ttgccgtttc ctccttccct ccggtcatga ttctctggga
     8461 tatggttacg ggacaggtgg aagccggcat gacggatgat cataaacttg acgatgccgc
     8521 ggtgcgtttt tcaccggacg gccggttgat cgccgtttcc gcgaccgata aggtttccct
     8581 ctgggatgcc gcgaagcaca gaaaaatcag aagcttcggc ggcgatgtcc agggaatcaa
     8641 tctggctttc ttttcagcgg accaggagca ggtcaaaatc gccggaaatg aggagctcgc
     8701 ggtgtttgac cgccgccagg gccgtttgac cggccgccgg acaatcgagc cattcgacaa
     8761 ctggtgggcg aaagggcaaa acgaatgggg caatcggcgc tttttcaaaa tcatcgacaa
     8821 gggctatcag gtgatcgaca gccgaacaga ccgggcgatc atcagcgacg ccgccgatcg
     8881 ctatccgacg gcctacgaca gcccgcaaca ggccgttact ttttctcccg acggcaggta
     8941 cgcgcttttg gcggagaaag acggaggaaa agtcagcctg ctcgatgtcc ggagtcgccg
     9001 ttcgatcgaa ctggtaaatt tccctgatca cgtgagtctg tcatcatctg acgggtacgc
     9061 ggctttttct ccggatggga gccttgttgc tgcagcatca tactctcatg atgaagaacg
     9121 gggagtcctg gtcatctgga atacggccac gggaaagcaa tccatcagac tgcgggcgaa
     9181 tcaaaaaagg gaaggcttta gccaggtggc ttttaccgcc gactcaaaac agatcgttgc
     9241 ggccgaaggc ggcaatcagg gcatcctcgg actctgggat ctgacctcgg gccgggaaat
     9301 aagatccttt ccgggtcatc ccggtttcat cacttctctg gcgctttccg gcgacggaaa
     9361 gttcgtcgtt tccggcgact ggatcggcac cgtgaaatta tggaatctcc gtcagggcgg
     9421 cgaggtcaaa acattcaagg gacatacgga cgagattaaa tccctggcct tctcgcggga
     9481 tggaaattac attctttccg caagcaagga cggcacggcg cgccagtggg atatcgcgtc
     9541 cggcaaagaa atcgcccaat tcgtcagctt caccaatggt gaatggattg tgatcacccc
     9601 ggaaggatat ttcaacgcct ctccgggcgg cgcaaaacac ctgaatgttc gcatcggatt
     9661 gaatgtctat tccatcgaca atctcttcga gaagtttttc aatcccgtat atgtggcctc
     9721 cgtgctgcag ggcaagaaag tggaagcgac ggccgacatc cgaaaaggca tcctttcacc
     9781 gccggaggtc cggatcacgt ctcccgcggc gggaagcgta ttgaatacgg atacgctggc
     9841 cgttaccgtg gcggccagag acacgggcgg cggcatcgac gaaattcggc tttatcataa
     9901 cggcaaggtt gtcggcggcg atcaacgcgc cgtcaaaatc gtcccccggg gcggggaaac
     9961 ggtccggcaa tattccgtgt ccctggtcga tggcgcgaat acgtttcggg ccgtgggatt
    10021 cagcagagac aggacggaat ccaatcctta tgaactgatg gtccggctta aggccccttc
    10081 caaggaagtg tctctttatg tcctggcggt cggcatcaac aaatacaaga atcccgcgct
    10141 caacctcaac tacgccgagc cggatgcaaa gagcattgcc ggcttcttca gtcagcgggg
    10201 cggcggattg ttcaggaaag tcaatatccg cgaaatttac agtgagcagg ccacccggga
    10261 gaatatcgtc tctcaattga acgagctgca aaataccaat ccacaggatg cagtgctgat
    10321 ctatctcgcg gggcatggtg agagcctgaa cgataaatgg tacttcatcc cgcatgaatt
    10381 gacctatccc gaacgggaag cggatgtgcg gacgaagggt gtttcatccg atgatctgtc
    10441 cggctatatc aaaaacatca aagcgcaaaa aatcctgctt ttggtggatg cctgcaaatc
    10501 gggcgccgtg ctgattgctt tccgcggatt tgaggaccgc aaagcgcttt cccagctatc
    10561 gcgctccacg ggcgtgcatg tcgtggccgc gtccaccaaa gaccagtttg ccgccgaagt
    10621 gaaagatctg gggcacggcg ttttcaccta tacgctgctt gcgggcttgg gcggcaaggc
    10681 ggcgggtccg ggcgaacccg tcaccgtccg caagctgatg gtttacgtgg aagagcagtt
    10741 gccggaaatc acaaaaaagt ataaacagga agcccagtac cccgtggtgg attcccgggg
    10801 aatggatttt ccgctggtgc ttggcagata aacgatgagg gaggtttcca tgcgtaaaat
    10861 tttatttctt gcggcaatga tcattctttt ggcggcgccg gtttatgccg cgcaatccac
    10921 catcaagatc gccgaaggct ccgcctgcat gggggacgac aagtcccgca agcagacgga
    10981 aaccgcggcg ctggcggatg caaagcgaaa cgccgtcgaa tcggtcaaga cctatgtttc
    11041 atccgcgacg gaggtgaaag acttcgagct gcagaaagac ctggtgtccg cttatgccaa
    11101 cgccaccgtc gaagttctcg agacgctgga aaagggctgg tataaagacc cggggcaggg
    11161 cgactgctac cggatcaagc tcaaagcgga ggtcattccg gatgaaaagg cgatgaaaaa
    11221 ggccggggca aaggctgcgg atgccgcgct ggatgatccg tctctgcctt tgaccgtgaa
    11281 ggtctggacg gacaaaacgt cttatgccgc gggagagaag atcagaattt atctcaaggg
    11341 caacaagcct ttctttgcgc gtgtgattta ccgggacgcg gtgaaaaaca acctgcagct
    11401 cctgcccaat ccctaccgcc gggacaatta cttccagggc ggcgtgattt acgaaatccc
    11461 ttccggcaag gatcagtttg agctggaagt gaacccgccc tttggcgagg aaaacatcat
    11521 cgtctatgcc agcgtctcgg agctgggcga tttgaagctg aaggatgagg gaagcatatt
    11581 cgccgttcaa accagatcaa aagacatcgg cgtgaaaaca aggagcgtca agatcaaggc
    11641 cgcgtcggac gacgcgggac tggccgcgga attttccgaa gtcaaagcgg tggtgaagac
    11701 gaggaagtag gggccgtctt tgcagccaac ggaaaaggag atggaagatt gaaaaaggca
    11761 tgggtgcttt ttttgtgctg gctgatcctg ttgggctctc cggcggttgt tcaggacgcg
    11821 aaagcacagg attcatcgcc gctgaaggtg gtcctgatct ttgacatggg cggcagggga
    11881 gacggcggtt ttaacgattc cgcatacagc ggcatggaaa aagcggttgc ggagctgggc
    11941 gtgaaggccg tctatgtcga gcataaacga aacctcgagc tggattatgc cgtaagcggg
    12001 gccgcggctt ccgacgcgga aatgatcatc ggcgtcggtt ttgccttttc ggaaaaattc
    12061 gatcatctgg ccgggcggca tccggataaa aaattcgtcg tggtggacta cagcgcgaat
    12121 tacgatgaga gaggaaacct catctcgccg ccgcccaatc ttgcggggct gacgtttagg
    12181 gaagaggaag ggtcctttct ggtcggggcg atcgcggctc tgaaaagccg gtccgggaaa
    12241 atcggtttta tcggcggaat ggacagcccg attattcgaa aatttcaggc agggtatgag
    12301 gcgggcgcaa aagccgttcg tccgggcatc cggatttatt cgcgctatgc aggcatcacc
    12361 ggacaggcct tcagggatcc gcggaaaggt taccggatcg cggtgagcat gtataaaaac
    12421 ggagcggacg tcatttatca tgccgcaggt gaaacgggag cgggcctctt tcaggcggca
    12481 agaaagatga atcgtctggc gatcggagcg gatatcgacc agagcgcgca ggcgccgggc
    12541 cgtgtcctga cgagcatgct gaaaaatatc gacggggcgg tgttcgaggc tgttcaatcc
    12601 tgcgctcacg gaaacttttc gggaggtctc aaaacgctgg ggctgaagga acggggcgtc
    12661 ggatttgttt acaataacca gaataagaag ctgattccag aaagcattca tcagcaggtt
    12721 caggcgctgc gggcaaaaat tgtgtcgggc gagctggcgg ttcccgaagc gagcggacac
    12781 cggacggcgc tttcccgaca ggaattgcgg gatcttttgt cccgtctgca atccgagatt
    12841 cagacgacgc tgaaccggtt ggacaatgat ttgcggcacg gcgcaaaggt gctggccggc
    12901 aaagacctgc ggggcgattt tgcgcgcggc attctccagg ggctttacag ggcaaatccc
    12961 tatatcatcg actgtgagac ggtgagcgat tcgggaatca tgctggcggt ggagccgccg
    13021 gcgcaccggt cgtccgaagg cgccgacatc agccgacagt cgcacatggt ccggctgttc
    13081 aaaacccaca cgcctgtatt gagcggctca ttccagtccg tggaaggccc ccgggcggtc
    13141 gtgattcatc atcccgtatt tttaaaagac cgtaaatttt cgggatcggt ctcggctctg
    13201 tttgcgccgg aatacctgct ttcgagcatg atcggaccga tcgccgccta catgccggtt
    13261 tccattttcc tgatgcagac cgacggcctt tttatttatg acgtggatgc gaaacagatc
    13321 ggactcaaca tcttcaccga tccgttatac cagccgtttc cggagctgat tgccctttgc
    13381 cgcagaatgg ccgatacccg cgaaggatcg ggcgtttacc gtttttaccg aaaagtcggt
    13441 gaagcgccga ttttcaaaac ggtggaatgg cgcaccgtgg aactgcacgg cacgccctgg
    13501 cgcctggccg ccgcctttgc cggagacggc gtcgaacgtt aaaatttata cgttccggcc
    13561 gtcacgttgc gatagatttc atcactgacg ggcacatagg tttcgctccc cggcagaagc
    13621 gcgtacagtt cctccctgac ctgcgccgga tcatagccct cattcttgag gttggtcttc
    13681 ttgattttgt gcgtggcggt ccattcaaaa tccggaacga aacggatgaa gatcggcacg
    13741 gcgtatttcg gcaggctcgc cttcaggtgg gctgccagcc gggccagatc gaacggctcg
    13801 ttttcatccc gaatgacggc ggccattccg gcctttccgt cgctgccggg catg
//
LOCUS       DAOSIM010000031        13825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_4524_length_13825_cov_7.086710, whole genome shotgun sequence.
ACCESSION   DAOSIM010000031 DAOSIM010000000
VERSION     DAOSIM010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 13825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13825
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4524_length_13825_cov_7.086710"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1087
                     /locus_tag="PLR47_02620"
     CDS             149..1087
                     /locus_tag="PLR47_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG11861.1"
                     /translation="MTTESSSQPILDEADRLIEEGKPGEAVALLEALHAREPDEEPVL
                     LRLAWASWDIGRKEQSIQYWEELLERELQRRIFTGFAYDELVRIYKQEALFEKLIAVC
                     EKAVAAQPDDAGLLAELGNAQLQSGRALEACRIFEKLTRMEEDNPDFLCRLGEALFAA
                     GRASESEAACLRAGRMDPEQIDRYSFRIADLYSRGKRHAEAIRLLERCIAANPSQPLY
                     YCSLGDELAALGRVDEAEAVYETAAAKSPSGAYYNRLGNTLMKEKMFDRAARAFEKAA
                     AREGLRPFFVNLARAYREMGLPEAAGEALRKAGETS"
     gene            complement(1084..1596)
                     /locus_tag="PLR47_02625"
     CDS             complement(1084..1596)
                     /locus_tag="PLR47_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11862.1"
                     /translation="MSEENAAAASGTAREMSEERPFGFIAPEGNTAMVCEPDAAVREK
                     IVSELKGMGYAVAEAKSAREALQFMRFHIFSAIFVNESFSTAEGQENSVLKFLEGLSM
                     TVRRQTFVVLISASLRTMDNMMAYNRSVNLIVNQNEIAEVGQVFTKALDEHESFYHVY
                     KENLRKAGKA"
     gene            complement(1635..2828)
                     /locus_tag="PLR47_02630"
     CDS             complement(1635..2828)
                     /locus_tag="PLR47_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOG11863.1"
                     /translation="MRKQEIDRILEHMLASHPNISDLNLTVNKPFQVESSGQLTGVDL
                     DPPFARLTPFQTETFALNLVNQDHRLTDDLVSTGSCDLSYELPGIARFRVNVFSQRGH
                     YSIVLRKLETKIPSCADLKLPEAFYNISREKNGIVLVTGSTGSGKSTSLAAVINEINE
                     QKSIHIITLEDPVEFTHQQKKATINQRELGKDFDTFSSGLRAALRQAPKVVLVGEMRD
                     RESVEIALSAAETGHLVLSTLHTVDAGSTINRILGMFPSEEENQIRIRLADTVRWIVC
                     QRLLPKEGGGRVAAFEALGMNLRVKDIIQNGESEGKTFYDVMEQSKAFGMITFDSCIV
                     DLYSKGLISEETAKAYASNKSNVNRGIDAIKNARGEKTTDLGKLEVDKSYGRPQAGAP
                     PWQKK"
     gene            complement(2847..3929)
                     /locus_tag="PLR47_02635"
     CDS             complement(2847..3929)
                     /locus_tag="PLR47_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOG11864.1"
                     /translation="MAKIDAFFQLMHEQGASDLHLVSGQQPVIRMRGDLERIKYDVLT
                     NDGLKALLYEITPEHKIKVFEETGDVDFAYEIPGLARYRANFFQQKYGVAAVFREIPS
                     KILTCEQLALPPVLSKLAQLPRGLVLVTGPTGSGKSTTLAAIIDEANRTRKDHIITVE
                     DPIEFVHQSQSCVVNHREVGVHTKTFSSALRGALREDPDIILVGELRDLETISLAVEA
                     ASTGHLVFGTLHTTSAPKTVDRIIEVFPSSEQAQIRSTLADGLRAVIAQVLFKRIDIK
                     GRCAALEILIATAAVRNLIREAKTFQIPSMMQTGKKYGMQLLDDAIMDYVNKGWISGE
                     EAYNKANDKAKFRPLLKSPPTDFTEA"
     gene            4195..4270
                     /locus_tag="PLR47_02640"
     tRNA            4195..4270
                     /locus_tag="PLR47_02640"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4228..4230,aa:Ala,seq:cgc)
     gene            complement(4350..5630)
                     /locus_tag="PLR47_02645"
     CDS             complement(4350..5630)
                     /locus_tag="PLR47_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOG11865.1"
                     /translation="MKNKNVFFKVFPGIALVVLFLVLIPVFPAAPAGAEKPTAISDAE
                     TDPAVWGKIYPLQYNSYLKSKEMAASPTGFGGSIKVQKSVMQPEMLMNFKGNAFSKDY
                     SEDRGHPYSMDDLKESKRVTPITPGSCMTCKTSNLRNIYKDMGWNYAKTPLAELLPVI
                     KHTIACANCHDPQGMQLRVSNPAFIEAMGKRGIDVAKAPRQDMRSYVCGQCHSEYFLE
                     PKTSRVIFPWDKGLKPQEIYAYYQNLPSGFEMDWQHPDSQARMLKAQHPDFELATNGF
                     HGKMGLACANCHMPSVQEGGVQYSSHWVTSPLKHIQASCRTCHQQDEPLILERVKTKQ
                     NDVFQLLHTAGQTIARAHEALGKAGAAPKVNKAELGKARELLRNAQWLWDFFAAENSM
                     GFHNADEAQGTLKQSNDLAKQAIGMARKAAGIKN"
     gene            complement(5848..6792)
                     /locus_tag="PLR47_02650"
     CDS             complement(5848..6792)
                     /locus_tag="PLR47_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOG11866.1"
                     /translation="MAEENRTRSKMIRLGRILRLFMEKEKITVAWLAGYFRTTPRTIQ
                     RDLMLLKESGFPLHEEKKGTYGFSRDLVKNLEVFDDTELALMVALKNIVGQLGKPFQK
                     AADDVLDRLYDCVTSMPVFVKIDDAIPMDASFINRMVKAIREKRQVGFRYTGNKKTHD
                     VQMEPYRVVYYGGFWYLIGNEPVTGILKRYALDKIKDLRLLTSGFKTIPENLDSVLKE
                     SANIWFAEKTHLTVTVLVDSQVSHYFKRRKMFPTQEIREERPDGSLVVVFRVGHFEAI
                     RDILKSWIPHISILEPEEFRRKLLAEVKDWVAKQTAAA"
     gene            6984..9386
                     /locus_tag="PLR47_02655"
     CDS             6984..9386
                     /locus_tag="PLR47_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOG11867.1"
                     /translation="MNIFQTLLPEGSPAAKSRKNVRVLGIDLGTTNSTIAEIIYNPTA
                     KDNVHIRCLAVEQPAGSRSHWHPLVPSVVALHDGREIIGEGARLLRSRCLEEGLIEKG
                     SLFAAVKNDMGLRRTYNMAPAGYRSAAEISGRIVAYLKNAALKDKAQAPARTSVTVPA
                     SFQTAQREDTLKAAALAKLAVRGGDLLDEPIAAFIGYLAEHSDRQLLEIGQTRHLLVF
                     DFGGGTCDVAIFRLSTDLSGKLMVSSLSVSRYNRLGGGDIDQAILYGVVLPQILEQNG
                     LDRHALDYNTKKTMIEPAFISVAEQLKTGLCEKLAGLGEQKDWPADLEETCHRKFSLT
                     LNDGREITLVNPTLNLKTFNEILAPFLNENMLFVRETEYYQTLSIFVPILDALHRSDL
                     KEDQIDLFLMAGGSCLIPHVKPKLHASFKNAEMLSFATADAMQTAVARGAALNALSLA
                     LSERPVIQPVCQESIAIVTGNGTLDLVSKGAPLPWPQNGGYQEITKLMLPECPADEDL
                     PIRVEVVAREEGGNRTLLSELWKIPSPVKAGDRIRLEYRYDENQVLDVRAYHAGRDDV
                     KPYQGRKEHPLTNISNPQAIRLRIDETEEKLRTGLIGHNERKNVIISLAEDCAELRQY
                     EKAAYLLADLLRQRNEPDAFILNRMAMYCGFLGDEASEEKLYAEASTVDPRMSAPRFN
                     LALLLQKQKRHEEAKAAVEKAILSQDSPPYYVLQASILRDMGKEFAVKEFLEMAWKRF
                     QKPSELNDWELGWYITAAKMRGDEDAAAAARRERNNRRSGCVNPHQETEGLLPLLESA
                     AT"
     gene            9399..9692
                     /locus_tag="PLR47_02660"
     CDS             9399..9692
                     /locus_tag="PLR47_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11868.1"
                     /translation="MKSAPVLKVLSNGWEVPYPEDAFFGDNLLLLKDYRLNSMPEKAW
                     TRSFLYDEYVEWLQAYLFRCDEIDLMTWQSWIPARIATKRYLDCASIGRHAGF"
     gene            9708..10184
                     /locus_tag="PLR47_02665"
     CDS             9708..10184
                     /locus_tag="PLR47_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11869.1"
                     /translation="MYHCYNFPENFEETFDIDHLKGTFDDDPYAEMLKRGLLDDDPPP
                     DRLKFILQEYGGLLLKKKDEEQFQRYWPGFVKLEPLHWAFGLAVDNLEPADSAWRVYL
                     SLVLKRINNKGARIKALDRFFIEYEDTSFTSKADASGKIKICIIDEVPRINQTLTE"
     gene            10391..11374
                     /locus_tag="PLR47_02670"
     CDS             10391..11374
                     /locus_tag="PLR47_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence protein RhuM/Fic/DOC family protein"
                     /protein_id="HOG11870.1"
                     /translation="MKHQISGENIQGKVILYKNTLEVRLDEKTVWLSQKQMSELFQKD
                     VRTINEHIKNIFAESELTSKSVIRKFRITASDGKSYETTHYNLDVIISVGYRVKSLRG
                     TQFRIWATNVLRKHLVDGYTINEKRLKAQQNKINELQNAVRLLGNIALLEGVSDEAKG
                     IVQIISEYWRALNILDDFDHQRLAYPKGTRKSKYKLTYEEARNIIEQMKRKFRDSALV
                     GQEKDKSFQGSIGAIYQTFDGKDVYPATEAKAAHLLYFVTKNHSFVDGNKRIAAALFI
                     CFLQKNEILLTKDGYKRIDDNALVALTLMIAASKPSEKGMMIKVILNLLKE"
     gene            11606..11923
                     /locus_tag="PLR47_02675"
     CDS             11606..11923
                     /locus_tag="PLR47_02675"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOG11871.1"
                     /translation="MLQFFRTYTDSLFLSQAVRELEKKGRKTPNPEELGDKPSIGIIL
                     CAEKDDVEVEYALKTKQNPIGVAEYHLQSKLPAEMKGKLPTARQLADVVRATLPEKKE
                     KRP"
     gene            12146..>13825
                     /locus_tag="PLR47_02680"
     CDS             12146..>13825
                     /locus_tag="PLR47_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11872.1"
                     /translation="MSQENSIDQLKYFLKEMFQFNANDLDFGIYRIYNLKRKEIENFI
                     DGSDEQCLEPIINETLDQVKNIERSVELASLHTYLAKLNQQDLLSHVEENYEQLKLFV
                     QANKDDKEKGELLNILESATKEFNITDEIKDKIYNHILGFFEMYYSNGDFGYNNRSRN
                     LYKIPYEADYDGSDTMFHWKHKGSLYIKTGASFNAVKFKLKHLDQEFELRLETNEESK
                     EEDTARNSNKDTKLKHYKFNRFEVVDRVTRLIFNLSDSSTTKADLFKDIFKCVFQSDV
                     ELDKYLFAADSKGHLKPVFNDLIDDYDKVLEGSVKGIGALRINRKTLLNKINKNFERG
                     GKIEIISTDGQEHFSDATLETLYNIDQKLNSFYIGNDSDYFIHENLQEFLSQEKERYI
                     KNHILGDLTSILDGKLDNTTIIIAKAFDLVSSCIIEFLSTIEEFQKKLFSMKKKVVES
                     EYCLTIDNIDEKYYDEILKNKAQLIAWENLFSFKVTSVAELKAQPTLVLDTKFFRQQD
                     GSNSFKDKILAEIDNLDERINGLLINSENYQALTLLEEKYRGAIKCIYIDPP"
BASE COUNT         3438 a         3570 c         3608 g         3209 t
ORIGIN      
        1 aaaaccgatt aacattgatt tcatcacaac cccgcaggga gcgggcttca aattaacgtc
       61 caacctcccc gccgcgggaa gcggctgcgg ttcctgcagt tgctgaacca gattccgggg
      121 gagaattttc tcccccgttt ttttcgccat gacgaccgaa tcttccagcc agccaatcct
      181 cgatgaagcc gaccgcctga tcgaggaggg aaaacccggc gaagccgtcg cccttctgga
      241 ggcgcttcac gcccgcgaac cggacgagga gcccgtactg cttcgcctgg cctgggcctc
      301 gtgggacatc ggacgcaagg aacaatccat tcaatactgg gaagagctgc tggagcggga
      361 gcttcagcgc aggattttta ccggctttgc ctatgatgaa cttgtgcgga tttacaagca
      421 ggaagccctg ttcgaaaagc tgatcgccgt ctgcgagaaa gccgtcgctg cgcagccgga
      481 cgacgcgggc ctgctggccg aactgggaaa cgcacagctt cagtcgggaa gagcattgga
      541 agcctgccgc atttttgaaa agctcacccg gatggaagaa gacaatcccg attttctgtg
      601 ccgtctgggc gaagcgctct ttgccgccgg gcgcgcatcg gagagcgaag cggcctgcct
      661 gcgggccggc cgcatggatc cggagcagat cgaccgctac agtttcagga tcgcggattt
      721 gtattcccgg ggaaagcgcc acgccgaggc gatacggctt ctggagcggt gcattgccgc
      781 caatccctct cagcctcttt actactgcag cctgggggac gagctcgccg cgctggggcg
      841 cgtcgatgaa gccgaagccg tctatgaaac cgcggctgcg aaaagcccat ccggcgccta
      901 ctacaaccgc ctggggaaca ccctgatgaa agaaaagatg ttcgaccgcg cggcccgcgc
      961 ctttgaaaaa gccgccgcgc gtgaaggcct gcgccccttc ttcgtcaatc tggcgcgggc
     1021 ctaccgggaa atgggtctgc ccgaagcagc cggggaggcg cttcggaaag ccggtgaaac
     1081 ctcttaagcc ttgcccgcct tgcgcaaatt ttccttatag acgtgataaa agctctcgtg
     1141 ctcgtccagc gcttttgtga aaacctggcc gacttccgcg atctcgtttt gattgacaat
     1201 caggttgacg cttcggttgt aggccatcat gttgtccatg gtcctgagcg acgcgctgat
     1261 cagcaccacg aaggtctgcc ggcgcacggt catggacaaa ccttccagaa atttcagaac
     1321 gctgttttcc tgaccttcgg ccgtgctgaa gctctcattt acgaatatgg cgctgaaaat
     1381 atgaaaccgc atgaattgca gggcttcccg ggcggacttc gcttccgcca ccgcgtatcc
     1441 catgcctttg agctcgctta cgattttttc ccggacggcg gcgtccggct cacaaaccat
     1501 ggcggtgttc ccttcgggcg cgatgaagcc gaaaggacgc tcctcggaca tctcccgcgc
     1561 cgtcccggat gccgccgctg cgttttcttc tgacataatc atctccttcg ctcgatattc
     1621 tttggcaaag tcatttattt tttctgccag ggaggggcgc cggcctgggg cctgccgtag
     1681 gatttgtcca cctccagctt cccgagatcc gtggttttct cgccgcgggc gttcttgatg
     1741 gcgtcaatgc cccggttgac gttcgatttg ttggaggcat aggctttggc cgtttcttcg
     1801 gaaatcagcc ccttggagta gagatccacg atgcagctgt cgaacgtgat cattccgaac
     1861 gccttgctct gctccatcac gtcgtaaaag gtcttgcctt cggattcgcc gttttgaatg
     1921 atgtctttca cccgcaaatt cattcccagc gcctcaaaag cggccacccg ccctccgccc
     1981 tccttaggca ggaggcgctg gcagacaatc cagcggaccg tgtcggccaa acgaatgcgg
     2041 atttgatttt cttcctcgga ggggaacatg cccagaatac ggttgatcgt ggagccggca
     2101 tccaccgtgt gcagcgtgct cagcaccagg tggccggttt ccgcggcgga cagggcgatt
     2161 tctacggatt cgcggtcgcg catttcaccg accagaacaa ccttgggcgc ctgacgaagc
     2221 gcggcgcgca gcccgctgga aaacgtgtcg aaatccttgc ccagctcccg ctggttgatg
     2281 gtggctttct tctgctgatg cgtgaattcc accgggtctt ccagcgtgat gatgtggatg
     2341 gatttctgct cgttgatttc gttgatcacg gcggccagcg acgtggattt tccgctgccg
     2401 gtggaaccgg tcaccagaac gatgccgttt ttttcgcgcg agatattgta aaaggcctcc
     2461 ggcagcttca ggtccgcgca ggacggaatc ttggtctcca gtttccgaag gacgatcgag
     2521 taatggccgc gctgggagaa aacattcaca cggaaccggg cgattcccgg cagttcgtag
     2581 gacaagtcgc aggagcccgt ggacaccaga tcgtcggtca ggcggtgatc ctgattcacc
     2641 aggttcaatg caaaggtttc cgtttgaaac ggcgtcaacc gggcaaaggg cgggtccaga
     2701 tcgacgccgg tcaactggcc ggaagactcc acctgaaaag gcttgttgac ggtcaggttc
     2761 aaatcggaaa tattgggatg ggacgccagc atatgctcca aaatacggtc gatttcctgt
     2821 tttctcataa atccacctcg cttgcgttat gcttcggtaa agtccgtggg cggacttttg
     2881 aggagcggac ggaattttgc cttgtcgttg gccttgttgt aggcctcttc gccgctgatc
     2941 caccctttgt tgacgtaatc cataatcgcg tcgtccagaa gctgcatgcc gtactttttg
     3001 ccggtctgca tcatggacgg aatctggaag gttttcgcct cgcggatcag gttgcgcacc
     3061 gccgccgtgg cgatcagaat ttccagcgcg gcgcagcggc ccttgatgtc gattctcttg
     3121 aacagcacct gggcgatgac cgccctgagg ccgtcggcca gcgtggagcg gatctgcgcc
     3181 tgttcgctgg aggggaacac ctcaatgatg cggtccacgg ttttgggcgc gctggtcgtg
     3241 tgcagggtgc cgaataccag atggccggtg gacgccgctt ccacggccag ggagatggtt
     3301 tccagatcgc gcaattcgcc gaccagaatg atgtccgggt cctcgcgcag cgccccgcgc
     3361 agcgccgagc tgaacgtttt ggtatgcacg ccgacttcgc ggtggttgac gacgcagctc
     3421 tggctttggt gaacgaattc aatcggatct tctaccgtta tgatatggtc cttgcgggtg
     3481 cggttggctt catcgatgat ggccgccagc gtcgtcgatt tgccggagcc cgtcggcccg
     3541 gtcaccagaa ccagtccgcg cggcagttgg gccagtttgg acagcaccgg aggaagtgcc
     3601 agctgttcgc aggtcagaat cttgctggga atttcacgga agacggcggc gacgccgtac
     3661 ttctgctgaa aaaagttggc ccggtagcgg gccagccccg gaatttcata ggcaaagtca
     3721 acgtctccgg tttcttcaaa caccttgatc ttgtgctccg gcgtaatttc atacagcaag
     3781 gctttcaagc cgtcgttggt cagaacatcg tacttgattc gttcaaggtc tccgcgcatt
     3841 ctgatgacgg gctgctgccc ggaaaccagg tggaggtcgg acgcaccctg ttcatgcatg
     3901 agctgaaaaa aagcgtcgat ctttgccata aatgtctccc gtcgataatt ttattttatc
     3961 ctatggtttg atggtccgga cggccctgcc cggcggctca tccccccgtc cgcacggcga
     4021 ttccgatgct ccatggaacg gttgcgagta tagggaaaac ggttcggttt tgtcaagaaa
     4081 agtttgggaa aaccgggcgc gttgcaaggc gttcagaacg cctcggcccc gcccttcaac
     4141 taattcttga cacttattca aacaatagat atgctccgcg cgtttttgcg gttcggggat
     4201 gtagctcagc tgggagagcg cggcgttcgc aacgccgagg ccaggagttc gatcctcctc
     4261 atctccacca gaccttaatc caatacaatc caaagaagtg caaaaaaacc cgcgtcaacc
     4321 gcgggttttt gctgcgcgac gcatcaaaat tagtttttta ttcccgccgc ctttctcgcc
     4381 ataccaatgg cctgcttcgc cagatcattg gattgtttca gcgtgccttg cgcctcatcg
     4441 gcattatgaa aacccatgga gttttccgcg gcaaaaaaat cccatagcca ctgagcgttg
     4501 cggaggagtt cacgcgcctt gcccagttcg gccttgttga ccttcggggc ggcgccggct
     4561 ttgccgagcg cctcatgcgc gcgggcaatg gtttgccccg ctgtgtgcag caattgaaaa
     4621 acatcgtttt gctttgtttt gacccgttcc agaatcaatg gttcgtcctg ttgatgacag
     4681 gtgcggcagg aggcttgaat gtgcttcagc gggcttgtca cccagtggga tgagtattga
     4741 actccgcctt cctgcacgga aggcatgtgg caattagcgc atgccaggcc catcttgccg
     4801 tgaaaaccgt tcgtcgccaa ctcgaaatcg ggatgctggg ctttgagcat tctcgcctga
     4861 gaatcgggat gctgccagtc catttcaaat ccggatggaa ggttctggta ataggcgtat
     4921 atttcctggg gcttcaatcc tttatcccag gggaaaatca cgcgcgaggt tttgggttca
     4981 aggaaatact cggaatggca ttgcccgcac acatagctgc gcatatcctg gcgcggcgct
     5041 ttggcgacgt cgatgcctcg cttccccata gcttcaataa aggcaggatt gctgacgcgc
     5101 agttgcatgc cttgcggatc atgacaattg gcgcaggcaa tggtatgctt gatcacgggc
     5161 agcagttcgg caagcggcgt ttttgcgtaa ttccagccca tgtctttata aatattgcgc
     5221 agattggacg ttttgcaggt catgcaggaa cccggcgtga tgggggttac tcttttcgat
     5281 tcctttaaat catccattga ataaggatgt ccgcggtctt ctgaataatc tttgctgaag
     5341 gcatttccct tgaagttcat cagcatctcc ggctgcatga cggatttctg gaccttgatg
     5401 gagccgccga atccggtggg cgaagccgcc atttctttgc ttttcaaata gctgttgtat
     5461 tgcagcggat agattttgcc ccacacggcg ggatcggttt ccgcatcgga gatggccgtc
     5521 ggtttttccg cgccggcggg cgctgccgga aatactggga tcaggacaag gaacagcaca
     5581 accaaagcaa tgcccggaaa aactttaaaa aatacatttt tattcttcac tttcattccc
     5641 ctttctttgc cttgcctccc gttggcaacc ggatcaccgt gacacttcat gcaattcgcg
     5701 ccattccgct gcggaagaat ccgccgttat ttactctgat ttactgatct caatgttgag
     5761 cgcattatga cacagcgaaa gagcctgagc aaattttttt tgccggcggc gatgaaatgt
     5821 cagttcgacc ctccccgccg gacaaaacta agccgccgcg gtttgctttg ccacccagtc
     5881 tttcacttca gccagcagct tccgccgaaa ctcttcgggc tccagaatcg agatgtgcgg
     5941 tatccaggac ttcagaatgt cgcgaatcgc ctcaaagtgg cccacccgaa agacgaccac
     6001 cagcgacccg tccggccttt cctcccggat ttcctgcgtc ggaaacatct tgcggcgctt
     6061 gaaataatgg ctgacctggg aatccaccag gaccgtcacc gtcaaatgcg ttttttcagc
     6121 aaaccagata ttggcgcttt ccttaaggac ggaatccaga ttctcgggaa tggtcttaaa
     6181 gccggaggtc aataacctca gatccttaat cttatcgaga gcatagcgtt tgaggatgcc
     6241 cgtgaccggc tcgttcccga tcaggtacca gaacccgccg taataaacaa cccgataggg
     6301 ctccatctgg acgtcatggg tctttttgtt gcctgtatat cggaatccta cctgtctttt
     6361 ctcccggatc gcttttacca ttcgattgat gaaggaagcg tccatgggaa tggcgtcgtc
     6421 aatcttgacg aacaccggca tggaggtcac gcagtcatag aggcggtcca gcacgtcatc
     6481 ggcggctttc tgaaagggtt tccccagttg tcccacgata ttcttcaggg ccaccatcag
     6541 cgccagttcc gtgtcgtcga agacttccag gttttttacc aggtcccggc tgaagccata
     6601 cgttcccttc ttttcttcat gaagaggaaa gccggattcc tttaaaagca tgaggtcccg
     6661 ctggatggtc cggggcgtgg tccggaaata accggcaagc caggcgacgg tgattttttc
     6721 cttttccatg aacagccgca aaatccgtcc cagacggatc attttcgagc gcgttctatt
     6781 ctcctcggcc atggcatcct cctccctggt attgaaatgc aaatacaagg caaatttgtg
     6841 gggaaaaaac cgtgaaatcg aaaataatac gacacaccgt tgtcgacttg cctttgaaat
     6901 agcagaagga aggccataaa atcaacacca aaaagggagg caaaataaaa acagacctat
     6961 tggaacagtg aaggaggtgc gcgatgaaca tttttcaaac cctgcttccg gaaggaagcc
     7021 ctgccgcaaa atcaagaaag aacgtccggg tgctgggcat tgacctgggc acgaccaact
     7081 cgaccattgc ggaaatcatc tacaatccaa cggcaaaaga caacgtccac attcgctgcc
     7141 ttgcggttga acagcccgcg ggcagcagaa gccactggca tccgctggtt ccctccgttg
     7201 tggcgctcca tgacgggagg gagattatcg gcgaaggcgc gcgtcttttg cgaagccgct
     7261 gccttgaaga gggcctgatt gaaaaaggca gtctttttgc cgcggtcaaa aacgatatgg
     7321 ggctgaggcg cacatacaac atggcgcctg cgggctaccg gtccgcggcc gagatcagcg
     7381 gacgcatcgt ggcgtatctc aaaaatgccg ccctgaagga caaggcgcaa gctccggccc
     7441 gcacttccgt cactgtgccg gcatcgtttc agaccgccca gcgggaggac accctcaagg
     7501 ccgccgcact ggcaaagctt gccgtccggg gcggcgactt gctcgatgaa cccatcgccg
     7561 ctttcatcgg ctacctggcc gaacattcag acaggcaact gctggagatc ggtcagacac
     7621 ggcatctgct cgttttcgat tttggcggcg gaacctgcga tgtcgccatt tttcgcctgt
     7681 ccaccgatct gtcgggaaaa ctgatggtct cttcgctgtc cgtttcccgc tacaaccggc
     7741 ttgggggcgg cgatattgac caggccattc tctacggggt tgtcctgccc cagattcttg
     7801 agcaaaacgg tctggaccgc catgccttgg attacaacac caaaaagaca atgatcgaac
     7861 cggccttcat cagcgtggcc gaacagttga aaaccgggct gtgcgagaaa cttgccgggc
     7921 ttggcgaaca gaaagactgg ccggccgatc tggaagagac atgccaccgg aaattttcac
     7981 tcacattaaa tgacggcagg gaaatcaccc tcgtcaaccc gactctgaat ctgaaaacct
     8041 ttaacgaaat ccttgcgcct ttcctgaacg aaaacatgct ttttgtgaga gagacggaat
     8101 attatcagac gctttccata ttcgtgccga ttctggacgc cctgcaccgc agtgatctta
     8161 aggaagatca gatcgacctg tttttaatgg ccggcggaag ctgcctgatt ccgcatgtca
     8221 agccgaagct gcatgcatcc ttcaagaatg cggagatgct cagctttgcc accgccgatg
     8281 cgatgcagac ggccgtggcg cgaggcgccg cgctgaatgc cctttctctg gcgttgagcg
     8341 aacggcccgt tattcagccg gtctgccagg aatccatcgc catcgttacc ggcaacggca
     8401 cgctggatct ggtcagcaaa ggagcgccgc ttccctggcc gcaaaacggc gggtatcagg
     8461 aaatcacaaa actgatgctg ccggaatgcc ctgcagatga agatctgccg attcgcgtgg
     8521 aggtggttgc gcgggaagaa ggcggcaacc gcacccttct gagcgaattg tggaagattc
     8581 cctccccggt caaagccggc gaccgcattc ggctggaata ccgttatgat gaaaatcagg
     8641 tcctcgatgt gcgggcgtat cacgccgggc gagacgacgt caaaccctat cagggccgca
     8701 aagagcaccc cctgacgaat atttccaatc cccaggccat ccgcctgcgc attgatgaaa
     8761 cagaggaaaa acttcgcacg ggcctcatcg ggcataatga gcgcaagaac gtcatcatca
     8821 gcctggcgga agattgcgcg gagctcagac agtatgaaaa agccgcatac cttcttgccg
     8881 acctcctgcg ccagcgcaat gagccggatg cctttatcct caaccggatg gctatgtact
     8941 gcggctttct gggcgatgag gccagtgaag aaaagctcta tgccgaagcg agcaccgttg
     9001 atcccaggat gagcgccccg cggttcaatt tggccctgct gctgcaaaag cagaaacgtc
     9061 atgaggaagc caaagcggcg gtggaaaagg cgatcctgtc ccaagattcg cctccttatt
     9121 acgtgcttca ggcgagcata ttgcgcgaca tgggaaaaga gtttgccgtg aaggaatttt
     9181 tggagatggc ctggaagcgc tttcaaaagc cttcggagct gaatgactgg gagctgggat
     9241 ggtacatcac cgccgccaaa atgcgcgggg atgaagatgc ggccgctgcc gctcggcggg
     9301 agagaaacaa ccgccggtcg ggttgcgtca acccccatca ggaaaccgag ggcttgctgc
     9361 cgctattgga aagcgcggcc acatgaggtg aaaatgaaat gaaatcggcc cctgttctca
     9421 aagttctttc caacggatgg gaagtgccct atccggaaga cgcattcttc ggggataatc
     9481 ttttgctgtt gaaagattac cggctcaata gcatgcctga aaaggcctgg accagatcct
     9541 ttctctatga cgaatatgtg gaatggcttc aggcttatct tttccggtgt gacgaaatcg
     9601 acctgatgac ctggcaaagc tggataccgg cccgcatagc aacgaaacgc tatcttgatt
     9661 gcgcctcgat cggccggcac gccggattct aagagagcgt gctttcaatg tatcactgct
     9721 acaattttcc ggagaatttt gaagaaactt ttgacattga tcatttaaag ggcacttttg
     9781 atgatgatcc ttatgccgaa atgctcaaaa gagggttgtt ggacgatgat ccgccgcctg
     9841 accggctgaa atttatactt caggaatacg gcgggctgct cctcaagaaa aaagatgagg
     9901 aacagtttca gcgctattgg cccggttttg tgaaactgga accgctgcac tgggcgtttg
     9961 gcttggcggt agataatctg gagccggccg acagcgcctg gcgggtctat ctgtcgcttg
    10021 ttctgaagag aattaacaac aaaggagcgc gcattaaagc attggacaga ttttttatcg
    10081 agtatgaaga cacatcattc acaagtaagg cggatgcatc gggcaagatc aaaatatgca
    10141 ttattgacga ggtgccgcga atcaatcaaa ctttaacgga ataaaataaa cccatgcgcc
    10201 actatccacc gcattttttc ttgcaatctt ttcaagaagt tgtaatattc agcgcgcttc
    10261 atcgggcaat gtcaaaatct catgcgagac agaaagatca acttcaagac gccgagtttt
    10321 acggatgatc ggctgagcaa catttgcaca gaaaaataag ttattgaatg cagacatttc
    10381 aaggagcgcc atgaaacatc aaatatccgg cgaaaatatt caaggcaaag tcattttata
    10441 taagaacact cttgaagtcc gtttggatga aaagaccgtt tggctgtcgc agaaacaaat
    10501 gtcggaactg tttcaaaaag atgttagaac aattaacgag catatcaaaa acatttttgc
    10561 cgaaagtgag cttacgtcaa aatcagttat ccggaaattc cggataactg cctccgacgg
    10621 aaaaagttac gaaacaacgc attataatct cgatgtcatt atttccgttg gttacagagt
    10681 taaatcattg cgcggcacac agttccgcat ctgggcgacc aatgtcctgc gcaaacacct
    10741 ggtggacggc tacaccatca atgaaaaacg cctcaaagcc cagcaaaaca agatcaacga
    10801 attgcaaaat gccgtgcgcc tcttgggcaa tattgcgctt ctggaaggcg tctccgatga
    10861 agcgaaggga atcgttcaaa tcatttccga atactggcgg gcgttgaata ttcttgacga
    10921 ctttgatcac cagcgccttg catacccgaa agggacgaga aaatcaaaat acaaactgac
    10981 gtatgaagaa gcccgaaaca tcattgaaca gatgaaaaga aaattccggg attccgcttt
    11041 ggtcggccag gaaaaagaca aaagctttca gggttccatc ggcgccattt accagacctt
    11101 cgacggcaaa gacgtttatc ccgccacaga ggcaaaggcg gctcatctgc tttactttgt
    11161 aacaaagaat cacagctttg tggacggcaa caaacgcata gccgccgcgc tcttcatctg
    11221 cttccttcaa aaaaacgaaa ttttgctgac caaggacgga tacaagcgaa tagacgacaa
    11281 cgcgcttgtc gctctcacgc tgatgatcgc cgccagcaag ccatcggaaa agggcatgat
    11341 gataaaagtg attttgaatc tgcttaagga atagaggatc ttatttgccc cataagaatt
    11401 ttgtataagt tcttgcagtg catttttaaa attgtaaggc ggcaggtgga attagaaaat
    11461 gaatgtttca agacctgatg gctgcttgag gaattctgga agctggaaca agaagcggaa
    11521 aaactgttgg agggtctggc aagatgacca aagccaaaga gctttccctg gcaccttcgg
    11581 tttctcaccg caaaatgtgt ggcggatgct ccagtttttt cggacgtaca cggacagcct
    11641 gtttctctca caggctgtga gagaattgga aaagaaaggg cggaaaacgc cgaaccccga
    11701 agaactcggc gacaaacctt ccatcggcat catcctctgc gcggagaaag atgatgtgga
    11761 agtcgaatac gcacttaaaa ccaagcaaaa cccgatcggc gtggccgagt atcatctgca
    11821 atcgaaatta cctgctgaaa tgaaaggcaa actgccgacg gcccggcaac tggccgacgt
    11881 cgtccgcgcc accctcccgg aaaagaaaga gaaacggcca tgaatgttgc acaaaatgtt
    11941 cctaaaaccg ccgggattgg cgacattata aaatctgcgc tggcgccgct ggcagctctc
    12001 ttgcttggag aatttcattt gcgtcataca attatgtaga aggtcttgca atgtttttaa
    12061 gaatttgtaa gatgactgac aatttaatat ttgtttgaat aactcgcatg taacagatat
    12121 cagcaaaaaa atattgagag gcgcaatgag tcaggaaaat tcgatagatc agttaaagta
    12181 ttttctaaaa gaaatgttcc agttcaatgc caacgatctg gatttcggaa tttacaggat
    12241 atataacctc aagcgcaagg aaatcgaaaa ctttatcgac ggcagtgatg agcaatgtct
    12301 tgagccgata ataaatgaaa cgctggatca agtaaaaaac attgaaagaa gcgttgaact
    12361 ggccagttta catacatatt tagcaaagct taatcagcaa gatttactca gtcatgttga
    12421 agaaaattat gaacagctaa agctctttgt ccaggcaaac aaggacgaca aggaaaaagg
    12481 cgaactctta aatattcttg agtctgctac caaagaattc aacattacag acgagatcaa
    12541 agacaaaatc tataatcaca ttctcggctt tttcgagatg tattactcca acggtgattt
    12601 tggttataac aaccgcagcc gcaatcttta caaaatacca tacgaagctg actatgacgg
    12661 cagcgacacg atgtttcatt ggaaacataa gggcagctta tacatcaaga ccggcgcttc
    12721 tttcaatgcc gtcaagttta aattaaagca tctcgatcaa gaatttgaat tacgcctgga
    12781 aaccaacgaa gaaagtaaag aagaggacac ggcgcgcaac agcaacaaag ataccaaact
    12841 caagcactac aaatttaatc gttttgaagt tgttgaccgc gtgaccagac ttattttcaa
    12901 tctttctgac agctcgacaa ctaaagctga tttgttcaaa gatattttca agtgtgtatt
    12961 tcaaagtgac gttgaactgg ataaatatct ttttgcggca gatagcaagg ggcatttaaa
    13021 gccagtgttc aatgacctga tcgatgatta tgacaaggtg ctggaaggca gcgtgaaagg
    13081 catcggtgcg ttgcgcatca atcgcaagac gttgctcaat aagatcaaca aaaactttga
    13141 gcggggcggc aagattgaga tcatttctac agatggacag gagcatttct ctgatgcaac
    13201 gctggaaact ctgtacaata ttgaccagaa gctgaatagt ttctacatcg ggaatgacag
    13261 cgattatttt attcatgaaa acctgcagga attcttaagc caggaaaaag aacgctacat
    13321 caaaaaccat attctcggtg atttgacgag tattctggac ggcaaactgg acaacaccac
    13381 tatcattatc gccaaggcgt ttgatctggt cagcagctgc attattgaat ttttaagcac
    13441 gattgaagaa tttcagaaaa agctcttttc gatgaaaaag aaagtagtgg aaagtgaata
    13501 ctgcctgacc atcgacaaca tcgatgagaa atattacgat gaaattctga aaaataaggc
    13561 ccaactgata gcatgggaaa atcttttcag ttttaaggtg acgtccgttg ccgaactaaa
    13621 agcacaacct acattggttc ttgataccaa attctttagg cagcaagatg gcagtaattc
    13681 ttttaaagac aagatactgg ctgaaattga taatcttgac gaaagaataa atgggctttt
    13741 gatcaattca gagaattatc aagcgttgac tttacttgaa gaaaaataca gaggagcaat
    13801 caagtgtatt tatatagatc caccc
//
LOCUS       DAOSIM010000032        13433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_4680_length_13433_cov_6.598296, whole genome shotgun sequence.
ACCESSION   DAOSIM010000032 DAOSIM010000000
VERSION     DAOSIM010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 13433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13433
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4680_length_13433_cov_6.598296"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>432
                     /locus_tag="PLR47_02685"
     CDS             <1..>432
                     /locus_tag="PLR47_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOG11873.1"
                     /translation="RKFSPTVSLYIRYRVGAVDEIPGQSGAAHFLEHMMFKGTTTIGA
                     KNYPAEKKLLSRIEKTGEQLDRERKKQGSADPRKIRRLEERLKKLQNGHLRDFIPNEI
                     DRLYTENGASGMNAATGQDMTTYYINLPANKIELWARIESDR"
     assembly_gap    433..442
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <443..1349
                     /locus_tag="PLR47_02690"
     CDS             <443..1349
                     /locus_tag="PLR47_02690"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOG11874.1"
                     /translation="LEERRQRVEASPDGKLYEAFLGRAYTTHPYGIPIIGLPMDLTFL
                     SPAAIRGIHRKYRSPGRICIAVVGDVDPSKTMDLIRRYFGRIPDAGAPSARIPSEPEQ
                     RAERKVEVEFDASPSMIIGYHKPAPPAQDDYVLDVLEAILGKGRTSRLYDKLVTERQI
                     ADSVSVVNGVPASRYSNLFTIFARPRHPHTNEELQAAIIEEIESIKTHSVTEEELARA
                     KNQLKMDYIKSLDSNSELASILSYYELLLGDYRYFSDYLPRIQKVTAGEVRAAAVRYL
                     TAENRTVAFLNRKPASRKGAETHEK"
     gene            1339..2769
                     /locus_tag="PLR47_02695"
     CDS             1339..2769
                     /locus_tag="PLR47_02695"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOG11875.1"
                     /translation="MKSKKTIFCTAGLLLALIFLAAPQAAHTAPPWIPPDQIRYPALE
                     FRMPQAQRIALKNGMFLFYLQDSELPLVSLTALVRTGTLHDPAGKEGLAEITASVMRT
                     GGTAKLGSGEMDRRLDFLAASPSISMSLDSASIHFIFLKSDLDECLDLLSRMLLEPAF
                     EEKKIQEALALKKEELRRTVDNPQTLAFREFNRLLYPGDPRGRYATSASLDNISRKDL
                     ISFHREYFSPANMLLAVSGDISREEAVKKIEQVFGDAKIPRLVHDIPAAPRQGGRGFF
                     LIRKPIPQSTVVGGELTISKNHPDFYAFEVLDFLIGSGGFSSRIFSAVRNEEGLAYSA
                     GSFYRARSNYGVFGTYAFTKTASTFQAISLIESILRDAAAGSIARAELDWAKRSIINS
                     FIFSFEQPRQIAFEQMKNEYEKLPADYLTTYREKIEAVTLEDIRRIAAAFLHENRRLT
                     LIVGDADRFGAWPENWPAPAQIVPQP"
     gene            2781..4025
                     /locus_tag="PLR47_02700"
     CDS             2781..4025
                     /locus_tag="PLR47_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HOG11876.1"
                     /translation="MMIDEVLFEKVSRRIDAWRGDMIDLQKALTAVPAVGPANGGDGE
                     MPKARTLQKHLSDMGFTIFRHFDAPDPSVSDGLRPNFLASIPGTNNARSVWIITHLDI
                     VPPGEPGLWSADPYHAYVKDDCVYGRGTEDNQQDMAASIFAARALLAEGIVPRSTINL
                     LFVSDEETSSEKGVQHLLALPEKIFGSGDWIVAPDSGDSEGSLIEVAEKGILWLGFKT
                     TGKQCHGSKPQLGINAFSAASSFVTQLTKLREIFDAQDPLFDPPVSTFEPTKKEANVG
                     NINTIPGEDVFCMDCRILPRYKLDDVLFEIEAIKREVEKNHGVTIEISIHQRVDPAPS
                     TPDDAPVVLALQNAVDRVYRVRAAAGGVGAGTVAAYLRKRGLPAAVWSKTNMKAHQPD
                     ENCPVDNMTGNAKVFAHLFLQA"
     gene            4154..5146
                     /locus_tag="PLR47_02705"
     CDS             4154..5146
                     /locus_tag="PLR47_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOG11877.1"
                     /translation="MTKSTKSARAEKISFADSNLLRNLCGEHDRHLKLLEKLEDVKIK
                     PWGNTLSISGEEAAVAVVSGVIRQLCDLMLKGLHVELSDFDYAHRILSQDIDFDLSHV
                     FLDTVFISSKKRVIAPKSVAQRRYIDSMRNADMVLAIGPAGTGKTYLAMAMAVSCLLE
                     RKVQRIILTRPAVEAGERLGFLPGDLAEKVNPYLRPLYDALYDMVDMEKANALIGKGL
                     IEVAPLAFMRGRTLNDSFIILDEAQNTTSEQMKMFLTRLGFCSKAVVTGDITQIDLPT
                     ERSSGLVEAAAVLRNIPAVRIVHFSQVDVVRHPLVADVISAYASWEKENKNQKS"
     gene            5155..7485
                     /locus_tag="PLR47_02710"
     CDS             5155..7485
                     /locus_tag="PLR47_02710"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOG11878.1"
                     /translation="MNNFLDSTSEKILSFFRKWKEKHPFSFSFLKDVIFQRWAIAILL
                     SAGLTALLAPQFYVSKPVYRHGMIVQENIKADREFLVEDIQSTRLKQTEAAAGVRPVY
                     EYDPETAMTIRAKIRQSFAQAAGQPEKPDGKTLLENSLGVALTQEEYAFLKANAFSDE
                     LLQKLSRVIFAFYDKTLVTASAFQNHEREKGITIVNTLTRQEENTKEVSSILHMKDTG
                     PALLRKVHASIRSDQPDARRASFSFLKKMIAPNLIRSPEATAQKTASAKEAVKPVYFQ
                     VQKNEMIVREGDKIGYLELAKLEALHKTAADNNISNLAVIAGIFCIVLFLTLLLYFWR
                     TRNWLKTSPRSNVDFLVFAIVALLQIFVVKAGIFISVAVNRAFPSLPVDACYFAIPFA
                     MGAMIIAVLVNRNVAMIMSVLTSFLISLLFEEKILFPLFSFLGSVAASYHIVNSRQRS
                     TFLKVGIFLGVVNIAAILSLNLLTGRLLEDLVLRLAMGLFGGLITGILVAGFTPVFES
                     LFGFITYIKLLELANLNQPLFQKMIIEAPGTYHHSIIVASLVESAAEAIGANALLAKV
                     SAYYHDIGKLAKPHYYIENQTGYDNRHDRLSPKMSALIIISHVKEGCELALQAKLGKP
                     IIDIIREHHGTSLISYFFDKAKKDKDESIRSLTESDFRYPGPRPQTKEAGLVMLGDVI
                     EASSRTLSNPTPARIRSLVRERIARIYTDGQLDECELTLSNLNTIAETFTRILTGIFH
                     HRVEYPEAPVKPKETNGKRESHENPNRKPAEAVENR"
     gene            7439..7909
                     /gene="ybeY"
                     /locus_tag="PLR47_02715"
     CDS             7439..7909
                     /gene="ybeY"
                     /locus_tag="PLR47_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOG11879.1"
                     /translation="MKIRIANLQKRLKIDNRRIRGRVARLLRLIDCAEKEISITFVDD
                     EAIRLLNRQYLRLDKPTNVLSFSLKEGECGMVNPGLLGDIVISADTALRDARKGSLTL
                     EEELLFLIIHGLLHLTGYDHVRTSRANALKMKKREQELFLLLTHSDIRSGGCHP"
     gene            complement(7900..9564)
                     /locus_tag="PLR47_02720"
     CDS             complement(7900..9564)
                     /locus_tag="PLR47_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOG11880.1"
                     /translation="MVKYKKYFLSNRVWIAFLLMACFGLAFADRAQAQFPLFQDSPTV
                     SAPAQTGLSANPPAKEGQNAAEEEEPADVEEEQDLMENALDILEVADNYWKNGDIENT
                     LNELDKAYALLLDTDGDVEIARQKDDLRLLISQRIMAVYSSRQGPTNGKASEIQLIMN
                     ADVEKEIRSFQGPEREFFTASYMRSGLYRELIVTELKKAGIPEELFWLPLVESGFKVS
                     AYSPARALGLWQFIPSTGYKFGLTRDEWVDERMDVLKSTRAAIAYLKELHAMFGDWLT
                     VLAAYNCGEGRVLRVISSQRINYFDRFWDLYSRLPHETARYVPRFLATLHIVRNPAKY
                     GFELQTSTDTLMNYETVKVGKMMKLSDVAAKMEVSEDYINVLNAELRHKITPDREYAF
                     KIPEGSQQKFQLAYNDIPAAEKPGNHYGSDTGKSSKAGAGRYIRHRVRPGETVASIAK
                     RYRVSRQAIYSCNRINKRKSLSRQTRYVRIPIHMAESPAVRQKASGGKSYAASGSMKT
                     YKVKKGDTLFRIATRFNVPVAKLKQANRLKGNAIKYGMTLKIPDQG"
     gene            complement(9682..10825)
                     /gene="prfB"
                     /locus_tag="PLR47_02725"
     CDS             complement(join(9682..10752,10754..10825))
                     /gene="prfB"
                     /locus_tag="PLR47_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139198277.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOG11881.1"
                     /translation="MSADSLQETIADLKKRIASIGECLDVGELELQVKDLEILCAKKD
                     FWDDPEKARSNLRKKTKLSESLESWQKFSRRIRETGNLWSMAFEENDEGLLADLEADL
                     RKLSAEVREEELKMMLASEQDPMNAIISIHAGAGGTEAQDWVEMLLRMYLRWAEKRNF
                     KTAIIDYLPGDEAGVKSVALTVEGEYAYGYAKAEIGIHRLVRISPFDAGARRHTSFAS
                     VFVYPEVDDEIRIDIRDDDLRIDTFRSGGKGGQHVNKTDSAVRITHLPTGIVVQCQNE
                     RSQHKNKSMAMKYLKSRLYERELRQKNEKLDEENKQKKDIAWGSQIRSYVLHPYKMVK
                     DHRTNLEIGNAQKVLDGDIDDFIQAYLMTASDRKSGAGNTPPVNKG"
     gene            complement(10812..12383)
                     /gene="lnt"
                     /locus_tag="PLR47_02730"
     CDS             complement(10812..12383)
                     /gene="lnt"
                     /locus_tag="PLR47_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOG11882.1"
                     /translation="MKNNGTNINRNRTGKMPASRDLFFALLCGLLYFLSFPKFGFGIV
                     AWIALVPLFIALNNASSLSRALVLGWAAGITAHIGLLYWISHVVVNYGYLPMSLGVIL
                     TILLAGYLSVYFALFAAGIVYFRGRVPLLLAAPVLWVCLEHVKSVLFTGFPWENLGYS
                     QHANLLFIQTADITGVPGLSFLIVLLNVVIFTLLTQKSKKSLAAALAVFLLWSGVYAY
                     GALRLRQIQESMNHAERMDVSLIQGNIDQAVKWNENFQAETIRIYEELSLRKAMAPEG
                     LVVWPETAVPFSFQEPGVLQERVSRLPSKTKSWLLFGSISYAAGSAGTDYFNSAFLLS
                     PKGELRGKYDKVHLVPYGEYVPLRKLFPFVGSLASGIGDFAAGKGFAPLRMDSQKIGV
                     MICYEGILPEAARRYKNAGAGLLLNITNDAWFGRTSAPYQHLSMSVFRAVETRLCLAR
                     AANTGISAIVDPTGRILARTGLFEPDQINGRIPMIQTPTVYAEYGDWFVWACFAGLAG
                     LFIWSLKRREKNVSG"
     gene            complement(12380..13195)
                     /locus_tag="PLR47_02735"
     CDS             complement(12380..13195)
                     /locus_tag="PLR47_02735"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOG11883.1"
                     /translation="MKKKVFNLLSWNSPEQVRRELFSVLAKARKSGVVDDASRKMIEN
                     ILNFTSTLVREIMIPRTDIVSISADDEPQDMMREMVEAHYTRIPIHRGSVDNIIGILN
                     VKDLLAAWSPQMSAAEILSHMTRPYYIPETKNAHLLFYELKNNNKHIAIVIDEYGGTA
                     GLVTLEDLLEEIVGDIRDEHEEHADSEELTQMADGSIVADGRTEIEKIEEQLAIQLDR
                     GRYETVGGLILYAIRRIPHQGENFQIEGMDITIENADERSIRKVKIKKAENTP"
BASE COUNT         2935 a         3930 c         3607 g         2951 t
ORIGIN      
        1 gcgcaaattc agcccgaccg tttccctgta catccgctac cgggtgggcg ccgttgatga
       61 aatcccgggg cagagcggcg cggcccactt ccttgagcac atgatgttca agggaacgac
      121 gaccatcggc gcaaaaaatt acccggcgga aaagaagctg ctttcgcgca ttgaaaaaac
      181 cggcgaacaa ctggaccgcg aacgcaaaaa gcagggttcg gccgatcccc ggaaaattcg
      241 ccgcctggaa gagcgtctca aaaagctgca gaacgggcat ctccgggatt tcatccccaa
      301 tgaaattgac cgcctctaca cggaaaacgg cgcatcgggc atgaacgcgg caacgggtca
      361 ggacatgacc acctactaca tcaaccttcc cgccaacaag atcgaactct gggcgcgcat
      421 tgaatccgac cgnnnnnnnn nntctggagg aaagaaggca gcgcgtggag gccagccccg
      481 acggcaagct ctatgaagcc tttttgggcc gggcctacac gacccatccc tacggcattc
      541 cgatcatagg attgcccatg gatctgacct tcctgagccc ggcggcgatc cgcggcatcc
      601 accggaaata ccggtcaccc gggcggatct gcattgccgt ggtcggggat gtcgaccctt
      661 caaaaacgat ggacctgatt cgccgttatt tcgggcggat tcccgatgcc ggcgcgcctt
      721 ccgcgcggat tccttccgaa ccggagcaaa gagctgagcg aaaggtcgag gttgagttcg
      781 acgcaagccc gtcgatgatc atcggctacc acaaaccggc gccgcccgcg caggatgatt
      841 atgtgctcga tgtcctggag gcgattctcg gcaaaggcag aaccagccgt ctgtacgaca
      901 agctggtgac agagcggcag atcgcggaca gtgtcagcgt ggtgaacggc gtgcccgcct
      961 cgagatactc caacctcttc acgatttttg cgcggccgcg ccacccccac actaacgaag
     1021 aacttcaggc ggccattatt gaggagatcg aatcgatcaa gacccattcc gtgacggaag
     1081 aggaactggc ccgggccaaa aaccagctga agatggacta catcaaaagc ctggattcca
     1141 attccgaact ggcgtccatt ctttcctatt acgaactgct tttgggggat taccggtact
     1201 tttccgatta cctgcccaga atacaaaagg tgacggccgg cgaggttcgg gcggcggccg
     1261 tccggtacct tacggcggaa aaccgcaccg tcgcgttttt aaaccggaag cccgcttcca
     1321 gaaaaggcgc ggaaacccat gaaaagtaaa aaaacgatat tttgcacagc cggactgctg
     1381 ctcgcattga tctttcttgc agcgccccag gccgcacaca ccgcaccccc ctggattcct
     1441 ccggatcaaa tccggtatcc cgcgctggaa ttccgcatgc cgcaggccca gcggattgcg
     1501 cttaaaaacg gcatgttttt gttttatctg caggacagcg aattgccgct ggtttccctg
     1561 acggcactgg tgcggacggg aactttgcac gaccccgcgg gaaaagaagg acttgcggaa
     1621 attaccgcct cggtgatgcg cacaggcgga acggcaaaac tcggaagcgg cgaaatggac
     1681 cggcgcctgg attttctggc cgcttctccg tccatttcca tgtcgctcga ttccgcctcc
     1741 attcacttca tctttttgaa aagcgacctg gatgaatgcc tcgatcttct ctcccggatg
     1801 cttctggagc ctgcctttga agaaaaaaag attcaggagg cgctggcatt aaagaaagag
     1861 gaactgcggc ggacggtgga caatccgcaa acactggcct ttcgcgagtt caaccggctt
     1921 ctctatcccg gcgacccgcg gggccgctac gcgacctccg cctccctgga caacatttcg
     1981 cgcaaggatc tgatttcgtt ccaccgcgaa tacttttcgc cggccaacat gctgctggcg
     2041 gtatccggtg acatctcccg tgaagaagcc gtaaaaaaaa tcgaacaagt tttcggcgat
     2101 gcgaagattc cccgcctcgt tcatgacatt ccggccgccc cccgacaggg aggccggggc
     2161 tttttcctga tccgcaaacc gatcccgcaa tccacggtgg tcggcgggga actgacgatc
     2221 agcaagaacc acccggattt ttacgctttc gaggttctgg attttctgat cggcagcggc
     2281 ggattttcct cccggatttt cagcgccgtg cgcaatgagg aaggcctggc ctacagcgcc
     2341 ggcagcttct accgcgcgcg gtccaactac ggtgttttcg gaacctacgc cttcaccaag
     2401 acggcttcga cctttcaggc gatttccctg atcgaatcca ttctgcgcga cgcggcggcc
     2461 gggtccattg ctcgggccga actggactgg gcgaagcgat cgatcatcaa cagcttcatc
     2521 ttttcctttg aacagccaag acagattgcc ttcgagcaaa tgaaaaacga atacgagaag
     2581 ctgcccgccg attacctgac gacctaccgg gaaaaaatcg aagcggtgac gctggaagac
     2641 attcgccgca tcgccgcggc ctttctccat gaaaatagaa gattgacgct gatcgtcggc
     2701 gacgcggacc gtttcggcgc atggccggaa aactggccgg cgccggcgca gattgttccc
     2761 cagccctgat ttgaggtttg atgatgatcg acgaagttct ttttgaaaaa gtatcccgca
     2821 gaattgatgc ctggcgcggg gacatgatcg acctgcaaaa agcgctgacg gccgttccgg
     2881 ccgtcggtcc ggcaaacggc ggcgacggcg aaatgcccaa agcccggacg ctgcaaaagc
     2941 atctgtccga tatgggcttt acgattttcc ggcattttga cgcgccggat ccctcggttt
     3001 cggacggact gcgcccgaac tttcttgcgt ccattccggg aacgaacaac gcccgcagcg
     3061 tctggatcat tacgcatctg gacatcgtcc ctccgggcga gccgggcctc tggagcgccg
     3121 atccctacca tgcctatgta aaggacgatt gcgtgtacgg ccggggcacg gaagacaatc
     3181 agcaggatat ggcggcttcc atttttgccg ccagggcgct gcttgcggag ggcatcgttc
     3241 cccgctccac gatcaacctg ctgttcgtat ccgacgagga aacctccagc gaaaaaggcg
     3301 tgcagcatct cctcgccctg ccggaaaaga ttttcggttc cggggactgg attgtggcgc
     3361 cggattcagg agattccgaa gggtcactga ttgaagtcgc cgaaaaaggc attctctggc
     3421 tgggctttaa aaccaccgga aagcagtgcc acggcagcaa accgcagctc ggaatcaacg
     3481 ctttttccgc cgcctcttcc ttcgtgacgc aactgacaaa gctgcgcgaa atcttcgacg
     3541 cacaagatcc tctttttgat cctcccgtca gcaccttcga acccacgaag aaggaagcca
     3601 acgtcggcaa catcaacacg attccgggag aagacgtctt ctgcatggat tgccgaatcc
     3661 tgccccggta caaactcgac gacgtgcttt ttgaaattga agccatcaag cgggaagtcg
     3721 aaaaaaacca tggcgtaacg attgaaattt ccattcatca gcgcgttgac cccgctcctt
     3781 ccacgccgga cgacgcgccc gtcgtcctcg ccctgcaaaa cgccgtcgac cgggtttacc
     3841 gcgttcgggc cgcggccggc ggcgtcggtg cgggaaccgt cgccgcctat ctgaggaaac
     3901 ggggcctgcc ggcggccgtc tggtccaaaa ccaacatgaa agcccatcag ccggacgaaa
     3961 actgcccggt ggacaacatg acgggcaacg caaaggtctt cgcccatctg tttttgcagg
     4021 cttaaattgt attgcaatgc cgttatcttg atgatagata gcctcctcgt tcaacggggc
     4081 catggccatc gcaaagatca ccggtaaaac ggttggacaa tggcggagat caacctttta
     4141 gaggcggatt tttttgacaa aatcaacgaa aagcgcgaga gcggaaaaga tttccttcgc
     4201 ggactcgaat ctgcttcgaa atctgtgcgg cgaacacgac cggcacctga agcttctcga
     4261 aaagcttgaa gacgtcaaaa tcaagccctg ggggaacacg ctttctattt ccggcgaaga
     4321 ggcggccgtc gcggttgtgt cgggcgtcat ccggcagctc tgcgacctga tgctcaaagg
     4381 gttgcacgtc gagctctcgg attttgacta cgcccaccgg attctttccc aggacattga
     4441 ttttgacctg tcccatgtat ttctggacac ggttttcatc tcctccaaaa aacgggtcat
     4501 cgcgccgaaa agcgtcgccc agcgccgcta cattgattcc atgagaaacg ccgacatggt
     4561 gctggccatc ggacccgccg gcacgggaaa aacatacctg gccatggcga tggccgtgtc
     4621 ctgccttctg gaaagaaagg tccagcggat cattctgacc cgccccgccg tggaagcggg
     4681 tgagcggctg ggtttcctgc cgggcgatct ggccgaaaaa gtgaacccct acctgcgccc
     4741 cctgtatgac gcgctctatg acatggtgga catggaaaaa gcgaacgcgc tgatcggcaa
     4801 ggggctcatc gaggtggcgc cgctcgcgtt tatgcgcggc cggactctga acgactcttt
     4861 catcatcctg gacgaagccc agaacaccac gtcggaacag atgaaaatgt ttctcacgcg
     4921 gctggggttt tgctccaaag cggtcgtcac cggcgacatc acccagattg atctgccgac
     4981 ggagagatcc tccggcctgg tcgaggcggc ggccgttctc agaaatatac cggccgtccg
     5041 cattgttcat ttttcgcagg tggacgtcgt ccgccacccc ctggtggccg atgtgatctc
     5101 cgcctacgca tcctgggaaa aagaaaacaa aaaccaaaaa agttgaacgt ggccatgaac
     5161 aatttcctgg attcgacatc ggaaaagatc ctgtcctttt ttcggaaatg gaaggaaaag
     5221 catcccttct ctttttcttt cctgaaggat gtcattttcc agcgatgggc gattgccatc
     5281 cttttgagcg cgggcctgac ggcccttctc gctccgcaat tctacgtttc caaaccggtt
     5341 taccgccacg gcatgattgt gcaggagaac atcaaggccg accgcgaatt cctggtggag
     5401 gacattcaat ccacaaggct gaagcaaacc gaagccgcgg ccggcgttcg tccggtctat
     5461 gaatatgatc cggaaaccgc catgacgatc agggcgaaaa tcaggcaatc cttcgcgcag
     5521 gcggccggac agccggagaa acccgacggg aaaactcttc tggaaaactc cctgggcgtc
     5581 gcgctcacgc aggaggaata cgccttcctg aaagccaacg cattttccga tgaactgctg
     5641 cagaaattat cccgggtgat tttcgccttt tacgacaaga cgctcgtcac cgcaagcgcc
     5701 ttccagaatc atgaacggga aaaaggcatt accattgtca acaccctgac gaggcaggag
     5761 gaaaacacca aagaggtgtc gtccattctg cacatgaaag acaccggtcc ggcgctgctc
     5821 aggaaggttc atgcctcgat ccggagcgac cagcccgacg ccagacgggc gtcgttttct
     5881 tttttgaaaa aaatgatcgc gcccaatctc atccgcagcc cggaagcaac ggcccaaaaa
     5941 accgcctccg cgaaagaggc cgtcaagccg gtgtatttcc aggtgcagaa aaacgagatg
     6001 attgtgcggg aaggggataa aatcggctat ctggagctgg ccaagctgga ggcccttcac
     6061 aagaccgccg cggacaacaa catctccaat ctcgcggtca tcgcgggcat tttctgcatt
     6121 gtcctgtttt tgaccctgct tctgtatttc tggcgcaccc ggaactggct gaaaacatcg
     6181 ccccggtcca acgtggactt cctggtgttc gccatcgtcg ccctcctgca aatctttgtc
     6241 gtgaaagcgg gaattttcat ttcggtcgcc gtcaaccggg ctttcccttc ccttccggtg
     6301 gacgcctgct atttcgccat tcccttcgcc atgggcgcca tgattatcgc cgttctggtg
     6361 aaccggaacg tggccatgat catgagcgtt ttaacctcgt ttctgatcag tctgcttttt
     6421 gaagaaaaaa ttctttttcc gcttttctcg tttctggggt cggttgccgc ctcctaccat
     6481 atcgtcaaca gccgtcagcg ctccactttt ttaaaggtcg gcatctttct cggcgtcgtc
     6541 aacattgccg ccattctcag cctgaatctg ctgaccggac gcctgctgga ggatctggtt
     6601 ctgcgtctgg ccatgggcct cttcggcggg ctgatcaccg gaatcctggt ggccggtttt
     6661 acccctgttt ttgaaagcct cttcggattc atcacgtaca tcaagctcct ggagctggcc
     6721 aatctcaacc agcccctttt tcagaaaatg atcattgaag cgccgggcac ctaccaccac
     6781 agcatcattg tcgcctccct ggtggaatcg gcggcagagg ccatcggcgc caacgcgctt
     6841 ctggccaagg tcagcgccta ttaccacgac atcggcaagc tggccaaacc gcattactac
     6901 attgaaaacc agaccggcta tgacaatcgc cacgacaggc tttctccgaa aatgagcgcc
     6961 ctgatcatca tttcccacgt caaggaaggc tgcgaactgg ccctgcaggc caagctgggc
     7021 aagccgatca tcgacatcat ccgggagcac cacggcacca gcctgatcag ctatttcttc
     7081 gacaaagcca aaaaagacaa ggatgaatcc atccgctcgc tgacggaaag cgatttccgc
     7141 tatcccggcc ccaggccgca gacaaaggaa gcggggctcg tcatgctggg cgacgtgatt
     7201 gaagcttcct ctcgcaccct ttccaacccg acgccggccc gaatccgttc gctggtgagg
     7261 gagagaatcg cccgcattta cacggacgga cagctcgatg agtgcgagct gacgcttagc
     7321 aacctgaaca ccatcgcgga aaccttcacg cggattttaa ccggcatttt ccatcatcgc
     7381 gttgaatatc cggaagcgcc cgtaaagccg aaggaaacga acggcaaaag agaatcccat
     7441 gaaaatccga atcgcaaacc tgcagaagcg gttgaaaatc gataaccgcc ggatccgcgg
     7501 ccgggtggcc cggcttttgc gtttgatcga ctgcgcggaa aaagaaatca gcatcacttt
     7561 tgtggatgac gaggccatcc gccttctcaa ccggcagtat cttcgcctcg acaaaccgac
     7621 caatgtgctc tctttttcac ttaaggaagg cgagtgcggc atggtgaatc caggcctgct
     7681 cggagatatc gtgatttccg cggacacggc gctgcgtgac gcacggaagg gttctttgac
     7741 tctggaggag gagcttctct ttctgatcat tcacggcctg ctgcacctga ccggctacga
     7801 ccatgtccgg acatcccggg ccaacgccct gaagatgaaa aaaagagaac aggagctttt
     7861 cctgctgctg acgcattccg acatccggtc cggcggatgt catccctgat cgggaatttt
     7921 cagcgtcatt ccgtatttga tggcgttgcc tttcagacga ttcgcctgtt tcagtttcgc
     7981 gacgggtacg ttaaatcgtg ttgcgatgcg aaacagcgta tcgcccttct tgactttgta
     8041 ggtcttcatg gagccggacg ccgcatagga ttttccgccg gaggctttct ggcggacggc
     8101 gggcgattcc gccatgtgaa tcgggatgcg gacataccgg gtctgccggc tcaaactttt
     8161 gcgtttgttg atccggttgc agctgtaaat cgcctgtctg gaaacccggt agcgcttggc
     8221 aatggaggcc accgtttcac cgggccggac ccggtgccga atatagcggc cggcgcccgc
     8281 cttgctgctt ttgcccgtgt cgctcccgta atggttgccg ggcttctccg cggccggaat
     8341 atcattgtac gccagttgaa atttctgctg ggatccttcg ggaatcttga aggcatattc
     8401 ccggtccggc gtgattttat gccgaagctc cgcgttgagc acgttgatgt agtcctcgga
     8461 cacttccatt ttggcggcga catcggacag cttcatcatc ttgccgacct tcaccgtctc
     8521 ataattcatc agcgtgtccg tcgaagtctg cagctcgaaa ccgtatttgg cagggtttct
     8581 gacaatatga agggtcgcga gaaaacgggg aacgtagcgg gccgtctcgt ggggcagccg
     8641 ggaatacagg tcccagaaac gatcgaagta attgatgcgc tgactggaga tgacgcgcag
     8701 aacccggcct tcgccgcagt tgtaggcggc cagtacggtc agccagtcgc cgaacatcgc
     8761 gtgcagctcc tttaaatagg caatggccgc ccgcgtggat ttcaggacgt ccatgcgttc
     8821 atccacccat tcgtcccgcg tcagaccgaa tttatagccc gtggacggga taaactgcca
     8881 caggcccagg gccctcgccg gggaatacgc gctgaccttg aatccgcttt ccacgagcgg
     8941 cagccagaag agctcctcgg gaatgcccgc ttttttcagt tcggtcacga tgagctcccg
     9001 atacaggcct gaacgcatgt aggaggccgt aaagaattct ctttccggac cctggaagga
     9061 gcggatttcc ttctctacgt ccgcattcat gatgagctga atctcgctgg ctttgccgtt
     9121 ggtgggtccc tgccgggacg aataaaccgc catgatgcgc tgggaaatca gaaggcgcag
     9181 atcatctttc tgccgggcaa tttccacgtc gccgtccgtg tccagaagga gcgcgtaggc
     9241 tttatccagt tcgttgaggg tgttttcgat atcgccgttc ttccagtagt tgtccgccac
     9301 ttccagaatg tccagcgcgt tttccatcag gtcctgctct tcttcaacgt ccgccggttc
     9361 ctcttcttcc gccgcgtttt ggccttcctt ggcgggcgga ttcgcggaaa gacccgtctg
     9421 ggccggcgct gaaacggttg gggaatcttg aaacagcggg aactgcgctt gggccctgtc
     9481 cgcaaaagcc aggccgaagc acgccatcaa gaggaaggcg atccataccc tgttggacaa
     9541 aaagtatttt ttatatttta ccatgcgtca gtatctcccg ttcatttgga tgcaagcccc
     9601 cctgatgcgg cgccgcttgt tttgcgcggg tttatagcac atttcgcccg catccccaaa
     9661 caatatcgac aaacgcaaat gttatccctt atttaccggc ggcgtatttc cggcgccgga
     9721 tttgcgatcc gatgcggtca tcagataggc ctgtatgaaa tcatcaatgt ctccgtcgag
     9781 cactttctgg gcgttgccga tttccaggtt ggtgcggtgg tccttcacca tcttgtaggg
     9841 gtgcagcaca taggagcgga tctgactgcc ccaggcgatg tcttttttct gcttgttttc
     9901 ttcatcgagc ttttcattct tctgccgcag ctccctttcg tagagccggg atttcaggta
     9961 tttcatcgcc attgatttgt ttttgtgctg cgagcgctcg ttctggcact ggacgacaat
    10021 gccggtcggc agatgggtga tgcggacggc ggaatccgtc ttgttgacgt gctgcccgcc
    10081 cttgccgccg gaccggaagg tgtcgatgcg caaatcatca tcccgaatgt cgattcggat
    10141 ttcgtcgtcg acttccggat aaacaaacac cgatgcgaag gacgtgtgcc gcctggcgcc
    10201 cgcgtcgaac ggcgaaatgc gcaccaggcg gtgaatgccg atctccgcct tggcgtaccc
    10261 gtaagcgtat tcgccctcga cggtcaatgc aacgcttttt acgcccgctt cgtcgcccgg
    10321 cagatagtca atgatggcgg ttttaaaatt ccttttttcc gcccatcgca gatacatgcg
    10381 cagcagcatt tccacccagt cctgcgcttc cgtgcccccc gcccccgcgt ggatggagat
    10441 gatcgcgttc atcggatcct gctcgctggc gagcatcatt ttaagctctt cttccctgac
    10501 ctcggcggaa agtttccgca ggtcggcttc caggtccgca agaagaccct cgtcattttc
    10561 ctcgaaggcc atcgaccaga ggtttcccgt ctcccgaatc cgccggctga atttttgcca
    10621 gctctccagc gattcggaga gctttgtttt ttttcgcagg ttggaacggg ctttttcggg
    10681 gtcgtcccag aaatcttttt tggcgcacaa aatctccaga tccttgacct gcaattccag
    10741 ttcgcctacg tcaaagacac tctccgatcg aagcgatcct ttttttcaaa tccgcaatgg
    10801 tttcctgaag gctatccgct gacatttttc tccctccttt ttaggctcca aatgaataaa
    10861 ccggccagac cggcaaagca cgcccacacg aaccaatcgc cgtactccgc gtaaacagtc
    10921 ggcgtctgga tcatcggtat tctgccgttg atttgatcgg gctcaaacag gcccgtcctc
    10981 gccagaatcc ttccggtcgg gtccacgatc gcggagatgc ccgtattggc cgcccgcgcc
    11041 agacaaagcc gggtttccac ggctcgaaaa acggacatgg aaagatgctg atagggagcg
    11101 gacgtccgtc caaaccaggc atcattcgtg atattgagca gaaggccggc ccccgcgttt
    11161 ttatatcgtc tggccgcctc gggcaggatt ccctcgtagc agatcatcac gccgattttt
    11221 tggctgtcca tccgcagggg agcaaacccc ttccccgccg caaaatcgcc gatgccggac
    11281 gcgaggctgc cgacaaaggg aaacagcttt ctcagtggaa catattcccc gtaaggcacc
    11341 aaatgcactt tatcgtactt cccccgaagt tctccctttg gtgaaagcag aaaggcgctg
    11401 ttaaagtaat ccgtgcccgc gctcccggcg gcataactta tggatccgaa cagcagccag
    11461 cttttcgttt tggagggcag ccggctgacc cgttcctgca aaacacccgg ctcctgaaag
    11521 ctgaaaggga cggcggtttc gggccagacc accaggcctt cgggagccat cgctttgcgc
    11581 agggacagct cttcgtaaat gcgaatcgtt tcggcttgaa aattttcatt ccatttaacg
    11641 gcttgatcga tgttgccctg aatcagggaa acgtccatgc gctctgcatg attcatgctc
    11701 tcctggatct gccgaaggcg cagcgcgccg taggcgtaaa ctcccgacca caaaagaaac
    11761 acggccagcg ccgccgccag ggattttttg gacttttgcg tcagcagtgt aaaaatgaca
    11821 acgtttaaaa gaacgatcag aaaagacagt ccgggcaccc ccgtgatgtc ggccgtctga
    11881 atgaacagca gattggcatg ctgggaatat cccaggttct cccagggaaa gcccgtgaac
    11941 aaaacggatt ttacatgctc caggcaaacc cacaaaaccg gcgcggccag aagcagggga
    12001 accctgcccc ggaagtaaac gatccccgct gcaaacagag cgaaatagac gcttaggtag
    12061 cccgccagga ggatcgtgag gatcacgccc agagacatgg gaagataacc gtagttcacc
    12121 acgacatgag aaatccagta cagcaggccg atgtgggccg tgatgcccgc cgcccatccc
    12181 agaacgagag ccctcgaaag cgatgaagcg ttgttcaggg cgataaaaag aggaaccagc
    12241 gcaatccagg cgacgatgcc gaagccgaac ttgggaaagg acaaaaagta cagaagcccg
    12301 caaaggagcg cgaaaaaaag atcgcggctg gccggcattt tgccggtccg attccggttg
    12361 atgtttgtgc cgttattttt catggagtgt tttcggcttt tttgattttc accttccgga
    12421 tgcttcgttc atcggcattc tcgatggtga tgtccattcc ctcgatttga aagttttcgc
    12481 cctgatgagg gattctgcga atcgcgtata aaatcagacc gccgaccgtt tcgtaccggc
    12541 cgcggtccag ttgaatcgcc agttgctcct caattttctc gatttccgtc cgtccgtccg
    12601 cgacgatgga accgtcggcc atttgggtga gttcttcgga atcggcatgc tcttcatgtt
    12661 catcccggat atcgccgaca atttcctcca gcaggtcctc cagcgtgacc agcccggctg
    12721 tccctccgta ttcatcgatg acgatggcga tgtgcttgtt gttgtttttc agttcgtaaa
    12781 acagcaggtg cgcgtttttc gtctccggaa tatagtaggg ccgcgtcata tgggacagga
    12841 tctcggcggc gctcatctgc ggcgaccatg ctgcgagcag gtctttgacg ttcaggatgc
    12901 cgatgatatt gtccacggag ccgcggtgga tgggaatgcg cgtgtaatgc gcctcgacca
    12961 tttcccgcat catgtcctgc ggctcgtcat ccgcgctgat cgacacgata tccgtccggg
    13021 gaatcatgat ctctcgcacc agggtggacg taaaattcag tatgttttcg atcattttcc
    13081 gggatgcgtc gtccactacg ccgctctttc gggctttggc aaggaccgaa aacagttccc
    13141 ggcgaacctg ttcggggctg ttccaggaca aaagattgaa aacctttttc ttcatagtct
    13201 cgattttccc cgcgcgcgac ccgcgtgaat tttcatcggc aattaaccac gcaacatccg
    13261 ttctgtcaat caccccgcgg aaaatcgccg ctcagagatg ccgttcatcg gcgatgggtg
    13321 aacaaagcgc ggcatcgctg ttttccgcag gatggaaagc cgcggcccgg ctttggggcg
    13381 aaataaaaag ggaaccactc aaaaaagctg attccctttt agattttctg gcg
//
LOCUS       DAOSIM010000033        13159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_4781_length_13159_cov_6.327305, whole genome shotgun sequence.
ACCESSION   DAOSIM010000033 DAOSIM010000000
VERSION     DAOSIM010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 13159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13159
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4781_length_13159_cov_6.327305"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1402)
                     /locus_tag="PLR47_02740"
     CDS             complement(137..1402)
                     /locus_tag="PLR47_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOG11884.1"
                     /translation="MSSDIIILLILFLFSGFFSSSEAALLSLSDLHLHKMKSDNYPFL
                     NSVVKLLEHPRRLLITVVTSNEAVNISISILAASLFIGLFGVSGQWISIVVTSVVLFL
                     AGEAIPKTFGVTYPMQVSSAVAPLLLWISRLEFPVVAALEKTPGLILKKLDQRKTRET
                     DVLMEDEFKHLIDAGSREGVVDESQKELIKTIFESGDRPVTDIMIPRVEMFCLALDMP
                     ASDIVREVVRGRYERVPVYREDRDDIIGILFARDLLNDALRRGESASIEKLLARPYFV
                     PESKTINGLLHAFQEKSIQIAIVVDEYGGVSGLVTLEDILEHLFEEAYGDDDAANCEC
                     DILDANTIIVPGKMSIEQVNELLQASLPQDEFDTIGGFVLHLFGKMPERGEKIQFEAY
                     HFRVESVGRTRILKIRIEKEAPAASEADE"
     gene            1699..2208
                     /locus_tag="PLR47_02745"
     CDS             1699..2208
                     /locus_tag="PLR47_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11885.1"
                     /translation="MKQHYLLLVLFVAVVLIVPPDLAAQELRSINLIPAPTRLKSDGK
                     TIEKPIAPRTGLIRDNVQEALKTWNNGDISSSLSDNFYDKSRFGDAMQTSIPRDARLR
                     VMDMGSMQILEQKIVKDPDGSQRRVTLGSVEVNSQIEFNDNKNGFVRVPGKNEIIYEI
                     SEKIKEEAP"
     gene            2205..2951
                     /locus_tag="PLR47_02750"
     CDS             2205..2951
                     /locus_tag="PLR47_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11886.1"
                     /translation="MIQRKTNCHSFSILAAFLLAALISFTGADALGETHTLKAPETYL
                     SINLPAKANAYSYYNPLSELTLEINGWACGHEIPDKVIFRLRNINYTLKLLTALNDSL
                     QNIETSFKKGELGKVGKDEIQHLVWAAIVETLKQTSFSSDRSLLESAENLIKEETENK
                     RRGMNNFFGYSNNYDMFAQKYYGRPGGVWTLAKMQPEEPPWAVFITEQCFSKSDLEMM
                     FNSVFQASQKLQEALKLYQETKARVFAVAQ"
     gene            3118..5844
                     /locus_tag="PLR47_02755"
     CDS             3118..5844
                     /locus_tag="PLR47_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11887.1"
                     /translation="MRPCDKVINLMNDLIRLGDAYQITQQNRENLYIEWIKAYNKGIG
                     DFIVVADAQNVAEPGRFMKEFLELKKIAAENGILAEGKMKDGQVAAVHSPDKDKYFYF
                     ADPDSAEGKSKQDLRYQLEENYEKLIERWNAARNDFYKQFEAEGLMSQHAKRLEEATK
                     EGREYADNYSKSRSELHTMALSGDFEHCLGEMKPLHYTIQNANVDFLQNSTNLQYAKG
                     ETNTPDSLPGMGYRIAPQKEYKPLIRPEISDFPFALHLKLNGEDTLRFERDLLKIKIK
                     AEEDETQIIGFGLGKTGQFLFDVTYKTTEALAGPVQMVQYAAAHPLDTFDKSVTFVKN
                     LDRTLGSIDFINLNRQAGDFIWGKAGNLAVAAVEIAKDVAPVSWDRFAPQPGESVQQE
                     IDRLNGQLVSTQKVKEGMNVASDIASIVLQAMADKGISKGLGATDDIIRGMRVEVKAS
                     EALKKLDTVESQLKLAKNAAALSDETRQTAKAVKELAEEQMKLNQKLQDMLKGNKQLP
                     KGTSVEGGRLIEAVDGQGNKVLLKEVEGDSLGKGGMNTVVKDADDSNMVLRKTNQPLS
                     KEDLAFQDAHDKFGRQVLEHEIKSDSLRVAKMEGEFFVETERGVIRYQKIEKVASDAA
                     GQIKDQGGVMTKGQQLAWEQARRDLNNSGYVWLDNTPKNFSFDKIPGGDDRWKIVVID
                     PDGIYPAAGKNPTVAKALQDAVDKTGKEGLTPMGGMYDLVVNFGTEAKKVGLQIDDVN
                     NAIDWDLMKKADPKMIPPGRELDADLLHWAPGQNRIRAAEDIAKLDDVGLKNSIDEFN
                     TGQISNDPDFKTLMEQQKAGQEKLDEALKKLEPEIKQAEQISDAGPKATVAPDKQGGL
                     DEKAAVGVLLVSQEAAAAIKAEKCALLRKQFLAGSQEKWLAEEVQKCVAAGY"
     gene            5754..7202
                     /locus_tag="PLR47_02760"
     CDS             5754..7202
                     /locus_tag="PLR47_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11888.1"
                     /translation="MRPFAQTVSGRFPGKMARGRGPEMRGRGILKEVCPMKRGFLIRR
                     FRVLQRIRKTYPAEKASLHSWFSNIPLAFLVFAVLAWPATAGSEEKKNPAEQVIEAPR
                     DFVEKNLTAYRKLLDILKNNHQEQFGSYLAATAIDPETIARDLPKGKELRLSIQYPEA
                     AAVKEEKISFPEGGSLLTENFRLAADVSPFYNLTASYSSDLTASGTEMLVAADPVTSQ
                     SLGLPYQVRISLRGDIIWTGISASASGSSCCLNASSRSVCIERNNAGRCKKTSSERYC
                     GKWADLPKTSNYRRSDLLLYANDLNLKAEASPGTKLGEGMKMATFSSPGLKKIRFTSS
                     RKYSNKEPYRIYHKCVPEFSASDKALAGYKAAGEEAAELQFTAVSLKGFGFAGYDISE
                     SYGEPPQLDFFYRDADVKSFTSARGNALLSPLVLLDYGDAGGLKAHPASKLNAPVTLT
                     VTGQGPFFISKKPRKLQRRNWRNQIPDNPGRQ"
     gene            7258..8787
                     /locus_tag="PLR47_02765"
     CDS             7258..8787
                     /locus_tag="PLR47_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11889.1"
                     /translation="MSKHDVVPGKNHKVLVQIKGPADMKRYEVRWSASGGAWRKETTD
                     FSLAGQEWQSEGAFLTPFAADNARQKGQPVKIALQIVDKKGGKALYTYENKLATTDAA
                     ISGLELHAGLNKRSARKVDEPIDLILGSTDPMVLLSPQVVLPDGEVFPLNLKAPSRLV
                     KIDSSNPEVASLRSERYGPDSLVKEIIVARPTGRLGTTQITARMGGSDYDKAGSAYLA
                     GNEEEIKSNTVNVSVNKAVLIADHQAGGRTAYRLIVTGPADMSKYLAIWRGEASRSTR
                     FAPCNNGYTSDLETTLTMEQVSVVKNNTVVASFNIKSGARKVNIKLLPVTPPFTVLEK
                     VALSDAGSLESITDCKKKITSMIDLFGFNPGMPVEDYCRSQREQEKQKIKTEREDQKT
                     FNEMIRNLDRQGQELVVVADSVKIGAAVKGDLAALDSAPFCSWRLERAGAITLAEAVT
                     PVETISANEGGCFNNLVGLKNGFNPDMTLRVELILLANPQTPLVITGESITAAGGILY
                     R"
     gene            8799..9566
                     /locus_tag="PLR47_02770"
     CDS             8799..9566
                     /locus_tag="PLR47_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11890.1"
                     /translation="MKIRFDITHSFLTLFFIFWAAVNGWAQSTVLDADSQQPFAGFGQ
                     YFVAPQEPEKQKETLPSSPSAAEGEAVFKILSITNISGNNPFHTTQPVLNFGGVVVSS
                     VDDLAECIRGGVCQSGKKETTMTCSPSSGISDLASWIESVGCYLKSNATVCRVEPLPS
                     SPDLSYETFFVMNDYRFAKSNVVGTASKSDYKLIIPPSGNTYVWPGGDTVYKIGTGTY
                     TLEVQHKVTGLRYRIEFTVQQLASNDIRLTVNSLVKL"
     gene            9593..11818
                     /locus_tag="PLR47_02775"
     CDS             9593..11818
                     /locus_tag="PLR47_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11891.1"
                     /translation="MRPNKNKRAESNCSIAVILLLFFFVAASPLCAEDPETPGEWIKT
                     SFDAWKINGSNTLRVDYYGNSGDETQATYPFTGLRSYDELNLNFSKSVSKYDTWRGEI
                     TGVLNNSPYRSNYYGIVPERLNLTKTKGDSLIPLRLEMGDIFSYYTYRTLQSSLKGVQ
                     LDLQPFQNSGSRHSFIITSGARQSSWRNFHLQDSYFNGASYMIDNAKFGKFSLNAVQN
                     SRQADEQQASLSRSQLIYSAAGNAIVKAVNQKLLLEGELSRFTGDHDGWDGPESGQKQ
                     SDNGLFFQGSGSSNWPLTYRVRYESYGRDYRPSAASVSPATKNAEGHLGWRLPKGYQM
                     RARAQKTTSNYEMNNPTDTIVYGFNLNGPLGSLDAFGQDISSDDKATDRITGTVSANI
                     NIPAIIGWKSRLGCQIQDLSDRIAGGVDVKTRQISFSADRSVHFGSFSGSVTPGILYR
                     QTESPTAKTDELLPTLIVGLKSGPHSLDYNLSWSDLQRPYDGGVDVGTVNQGFNYRYT
                     TERSVLGVEILYGQRDPDPGQKTGSYKLGAYWTFYFDKPAKTTTGRKPKPEEQALVQD
                     SESAIPALFNVDIAALIPGMKIEKAVELLKAKGIVNPVRDDVYLTYETRVLEEIEQRQ
                     RLVLIQENKILKKALVIIDFGAIDRPEDMLQTYQLVQSILLDKYGNPTSVTSRGELTQ
                     TFFQDVRDGKFVRSNDWERPGGKLRFCIPKRLDGMVRLSIEFAVSFPPPAEMWDDATF
                     R"
     gene            11950..>13159
                     /gene="purD"
                     /locus_tag="PLR47_02780"
     CDS             11950..>13159
                     /gene="purD"
                     /locus_tag="PLR47_02780"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOG11892.1"
                     /translation="MSNILLVGNGAREHAIAEAIARSPQNPRLFSFMKANNPGIASLS
                     EKIQPGSYADLAAITGFATANEIEFAVIGPEDPLNNGVVDALAQIGIPAVGPNQSLAR
                     LETSKSFTRNLVSKYNIPGNPQFKVFTSMDGVEAFLNALEGIVLKPDGLTGGKGVLVQ
                     GDHFATKEEALKLCTQILKDSFSVIVEEKFDGEEFSLQCLCDGKTVVGTPLVQDHKRR
                     FDGDRGPNTGGMGSYSLPDHSMPFLKPSDIAEGLEITRQVAAALLKETGNPYKGVMYG
                     GFIATKNGAKLLEYNARFGDPEAMNILPLLKTDFLEVCRHIIDGTLDKLKIEFEPKAT
                     VCKYIVPKGYGLPADHPDASSSKAKIAVGDVGAARLYYSSVDKREDGLYLSSSRAIGI
                     VGIADTLDAAR"
BASE COUNT         3620 a         3405 c         3297 g         2837 t
ORIGIN      
        1 cgggcggcgg acgatccccg acgcgcataa tatttaatct tgttgatgtc ggaagaaaac
       61 aacgcaactt ctcccccgga atacagagct tccaaaatca gacaaagcac gatgacggcg
      121 atcatgagat agaggctcat tcgtccgcct ccgatgcggc tggtgcttct ttttcaatcc
      181 ggattttcag gatgcgcgtt ctgcccacgc tctcgacgcg aaaatgatag gcttcgaact
      241 ggatcttttc gccgcgttca ggcattttcc cgaataaatg cagaacaaag ccgccgatgg
      301 tgtcaaattc atcctgaggc agggatgcct gcaacagttc gttgacctgt tcgatcgaca
      361 ttttgccggg cacaatgatc gtgttcgcgt ccagaatatc gcactcgcaa ttggccgcat
      421 catcatcacc gtaggcttct tcaaacaaat gctccagaat gtcttccagc gtcaccagac
      481 cggacacgcc gccgtactca tccaccacaa tggcgatctg aatcgatttt tcctgaaagg
      541 catgcagcag gccgttgatg gttttgctct ccggaacaaa atagggtctg gccaggagtt
      601 tctcaatgga ggcggattcc ccgcggcgca aagcatcatt gagcaaatcg cgggcaaaga
      661 gaatgccgat aatgtcgtcg cggtcttccc ggtaaacggg cacccgctca taccggccgc
      721 gaaccacctc ccggacaatg tccgaagccg gcatatccag cgcaagacaa aacatctcca
      781 cgcgggggat catgatatcc gtcaccggcc tgtctcccga ttcaaaaatc gtcttgatga
      841 gttctttttg agattcatcc accacgcctt cacggctgcc cgcgtcaatc aggtgtttga
      901 attcatcctc cattaaaaca tccgtctccc gggttttgcg ctgatccagc ttttttaaaa
      961 tcaaaccggg cgttttctcc agcgcggcga caaccggaaa ttccagacgg gaaatccaga
     1021 gcagcagcgg cgccacagcc gaggaaacct gcatgggata cgtcacgccg aaggttttgg
     1081 gaatggcctc gccagccaga aaaagaacaa cggaggttac gacgattgaa atccattggc
     1141 cgctgacgcc gaacaagccg ataaacagcg aagccgccag aatggaaatg ctgatgttga
     1201 cggcttcatt gctggtcacc acggtaatga gcaggcgacg gggatgctcc aggagcttca
     1261 ccacggaatt gagaaaagga taattgtccg atttcatctt gtgcagatga agatccgaaa
     1321 gcgacaggag ggccgcctcc gaagacgaaa aaaaaccgga aaataaaaaa agaatgagca
     1381 gaataataat atcggaactc aaaatgaatc aattcacctc gctggactgt ttcatgcttt
     1441 ttaatttatc agattccatc aaaaaatgat acaaagcttt ccaaaagcga ttatcttcca
     1501 aaataatgtc aaattgcaaa tcattgtcat gatcaattga tattttttag actctgaaaa
     1561 tattttaaaa tattcttgac aggacaagat atgtttttta taagcaacaa tctagagaaa
     1621 ttatcattta tataacctta acctgagttc cttttttatt tgtatcacat tttaaaatca
     1681 ggagggagtt accagctaat gaagcaacat tatctgctcc ttgtcctttt tgtcgctgtg
     1741 gttcttatag tgccgccgga tctagcggcc caggaactgc gctctataaa tttaattcca
     1801 gctcccacac gcctcaagtc tgacggaaaa acaattgaaa agcccatcgc tccccggacg
     1861 ggccttatcc gcgacaacgt gcaggaggcg ctcaaaacct ggaataacgg cgacatttcc
     1921 tctagtcttt ccgataattt ctacgacaaa tcgcgctttg gcgacgccat gcaaaccagc
     1981 atacctaggg acgcccgcct gcgggttatg gacatgggat ccatgcaaat tctggagcag
     2041 aagattgtta aggatcccga cggttcccaa aggcgcgtta cgctcggttc cgtggaggtc
     2101 aactcccaga tagaattcaa cgacaacaaa aacggctttg tccgggttcc gggaaagaac
     2161 gaaatcattt acgaaatatc tgaaaaaata aaagaggagg cgccatgatc caaagaaaaa
     2221 cgaactgcca ttctttttcc atcctcgccg cctttcttct tgccgccttg atttccttta
     2281 ccggcgccga cgccttgggc gaaacccaca ctctaaaagc tcccgaaact tatctttcca
     2341 taaacctgcc cgcaaaagcc aatgcttact cctattacaa tcccctgtcg gagctcaccc
     2401 tggaaattaa cgggtgggcc tgcggtcatg agattccgga taaagtgatc ttcaggctga
     2461 gaaatatcaa ttacacactg aagcttctga cggcgctaaa cgattctctg cagaatatag
     2521 aaacatcatt caagaaagga gaattgggaa aagttggcaa agacgagatt caacacctgg
     2581 tttgggcggc aattgtggaa actttgaaac aaacctcttt ttcatcggac cgttcccttc
     2641 tggaaagcgc ggaaaatctg attaaggaag aaaccgaaaa caaacgtcgg ggcatgaata
     2701 attttttcgg ctattccaat aattacgaca tgtttgcgca aaaatattac ggtaggccag
     2761 gcggtgtctg gaccctggcc aaaatgcagc ctgaagaacc accctgggcg gtttttatca
     2821 ccgaacaatg cttttccaaa agtgatctgg agatgatgtt caattccgtg tttcaagcgt
     2881 cgcaaaagct tcaggaggcg ctcaaacttt accaggaaac aaaagccaga gttttcgccg
     2941 tggctcaata ggactacatc tatgaaatca tacgcaagaa agacattttt ttcttttgga
     3001 ttacccgctg tgataatcgg attgattctg ttttcaacca tattttgcgc cgccgcggaa
     3061 ccgcaaaaac cggataacgg cctcgccgat agaatccgcc gcatggaagc aaggctgatg
     3121 cgtccctgcg acaaagtgat caatctcatg aacgatttaa ttcgactggg agatgcttac
     3181 cagatcaccc agcaaaaccg ggaaaacctc tacattgaat ggatcaaggc atacaacaaa
     3241 ggcatcggcg attttatcgt tgtcgccgat gcgcaaaatg tggccgaacc gggtcgattt
     3301 atgaaggaat ttctggaact taaaaagatc gcggcggaaa acggcattct ggccgaaggc
     3361 aagatgaaag acggtcaggt tgccgcggtg cattctccag acaaagataa atacttttat
     3421 tttgcggatc cggattcagc cgaggggaaa agcaaacaag acctgcgcta tcaactggaa
     3481 gaaaactacg agaagttgat tgagcgctgg aatgccgccc gcaatgattt ctacaaacag
     3541 tttgaagccg aagggctgat gagccagcac gccaaacgcc ttgaagaagc gaccaaagaa
     3601 ggcagggaat atgctgacaa ctacagcaaa agccgttcgg agctgcacac catggcctta
     3661 agcggcgatt ttgaacactg tctgggcgaa atgaaaccgc tgcattacac gatccagaat
     3721 gcaaatgtcg atttcttgca aaattcaacc aaccttcagt atgccaaagg tgaaactaac
     3781 acgccggact ctctgcccgg catgggctac cggatcgctc cccaaaagga atacaagccg
     3841 ctgatccggc cggaaatcag cgattttccc tttgccctgc accttaagct caacggagaa
     3901 gacacactgc gctttgaaag agatcttcta aaaattaaaa taaaagcgga agaagatgaa
     3961 acacagatca ttggcttcgg cctgggaaaa accggacaat tcttatttga tgtgacctat
     4021 aaaaccacgg aagccctggc cggaccggtg caaatggttc aatatgctgc cgcacatccg
     4081 ctggatacat tcgataaatc cgtcaccttc gtcaaaaacc ttgaccggac cctcggcagc
     4141 atcgatttta ttaatttaaa ccgtcaagct ggagatttta tctggggcaa agcgggaaac
     4201 cttgccgtgg cggcggtgga aatcgccaaa gatgtcgctc ctgtttcatg ggatcgtttt
     4261 gccccccagc cgggtgaatc ggtgcaacag gaaatcgacc gccttaatgg acagcttgtt
     4321 tccacacaaa aagtgaagga aggcatgaat gtcgcctccg acatcgcctc tattgtttta
     4381 caggcgatgg ccgataaggg catctccaag ggattgggcg caaccgacga tatcatccgc
     4441 ggcatgcgcg tggaagttaa ggcctcggaa gcattaaaga aactcgacac cgtcgaaagt
     4501 cagcttaaac tggccaaaaa cgccgccgct ctttccgacg agacccggca aacggctaaa
     4561 gccgtcaagg aactggccga agagcagatg aagctcaatc aaaaacttca ggacatgctg
     4621 aaaggcaaca aacaattgcc caaaggaacg tccgttgaag gaggccggtt aatcgaagcg
     4681 gtggacggtc agggaaataa agttttacta aaagaagtcg aaggcgactc actgggaaaa
     4741 ggcggcatga ataccgttgt aaaagacgcg gatgattcaa atatggtgct cagaaaaacc
     4801 aaccaacccc tgtccaaaga agatctggcc tttcaggatg ctcacgacaa attcggacgc
     4861 caggttctgg agcatgaaat taaaagcgac tcccttcggg ttgccaaaat ggaaggagaa
     4921 ttttttgttg aaactgaacg gggggtaata cgctatcaga aaatcgaaaa ggttgcctcc
     4981 gatgcggcgg gacagattaa agatcaaggg ggcgtcatga ccaaggggca gcagttggct
     5041 tgggagcagg cccggcgcga tctgaacaat tcaggttatg tctggctgga caacacgccg
     5101 aaaaacttca gtttcgacaa gattcccgga ggagatgacc gctggaaaat tgtggtcatt
     5161 gaccccgacg gcatttaccc ggcggcgggc aagaacccga cggtggccaa ggctcttcag
     5221 gacgcggtgg acaagaccgg caaagaaggc ctgacaccca tgggagggat gtatgatctg
     5281 gtcgtcaatt ttggaacaga agccaaaaaa gtcggcctgc aaatcgatga cgtcaacaat
     5341 gctattgact gggacctcat gaagaaggca gaccccaaaa tgattccgcc gggcagagaa
     5401 ctggatgctg atcttttgca ctgggcgccg ggacagaatc gcatccgggc ggcggaggat
     5461 atcgccaaac tcgacgatgt cggcctgaaa aattccatcg atgaattcaa cacagggcaa
     5521 atttccaatg atcctgattt taaaaccctg atggagcagc aaaaagcagg gcaggaaaaa
     5581 ctcgacgagg cactgaaaaa actcgaaccg gaaatcaaac aggccgaaca gatatctgat
     5641 gccggaccga aagccacggt tgctccggat aaacagggcg gtctggacga aaaagcggcg
     5701 gtgggtgtct tactggtcag ccaggaagcg gcggcggcga tcaaggcgga aaaatgcgcc
     5761 cttttgcgca aacagtttct ggccggttcc caggaaaaat ggctcgcgga agaggtccag
     5821 aaatgcgtgg ccgcgggata ttaaaggagg tatgccctat gaaaagaggc tttttgatac
     5881 ggcgctttcg tgttttgcag agaatccgca aaacatatcc tgccgaaaaa gcaagtcttc
     5941 attcctggtt ttcaaatatc ccgctggctt ttcttgtttt tgccgttttg gcatggcccg
     6001 caacggccgg aagcgaagaa aaaaagaatc ccgccgaaca ggttatcgaa gcgcctcgtg
     6061 attttgtgga aaagaacctc accgcatacc gtaaactttt ggatatacta aagaataacc
     6121 accaggagca gtttggttcc tatctggccg ccacggcaat tgacccggaa accattgcga
     6181 gggacctgcc caaaggaaaa gagcttcgcc tctctatcca atatccggaa gcagcggccg
     6241 tcaaagagga aaaaatatct ttccccgagg gcggttcgct tttaacggaa aatttccgat
     6301 tggcggctga cgtatccccc ttttacaatc taaccgccag ctacagctca gacttgacgg
     6361 cttccggaac tgaaatgctg gtcgccgccg atccggtgac gtctcaaagc ctcggcctgc
     6421 cttatcaggt tcgcatatca ctgcggggag atatcatctg gaccgggata agcgcttctg
     6481 catccggatc gtcgtgctgc ctgaacgctt cctcacgttc tgtctgcatc gaaagaaaca
     6541 atgcgggaag atgcaaaaag acgtcatctg aacgctactg cggcaaatgg gcggacttgc
     6601 ccaaaacctc caattaccgc cgctccgatc tgctgcttta cgccaatgat ttgaacctga
     6661 aggcggaagc gtccccgggg acaaagctgg gagaaggaat gaaaatggcc acattttcaa
     6721 gtccgggctt aaaaaaaatc cgttttacct cttccagaaa atattcaaac aaagaacctt
     6781 accgaattta ccataaatgt gttcccgaat tttctgcatc agacaaagcc ctggccggat
     6841 acaaagccgc tggagaagaa gccgccgaac ttcaattcac ggcagtgtct ctcaagggat
     6901 tcggttttgc cggttatgat atcagcgaat cctacggcga accgccgcaa cttgactttt
     6961 tttacagaga cgcagacgtt aaaagcttca cctcagccag aggtaatgcg cttctttcgc
     7021 ctctggtgct ccttgattat ggagatgccg gcggcctgaa ggcccacccc gcaagcaagc
     7081 taaacgctcc ggtgactctg accgtaaccg gtcaggggcc tttctttatc tctaaaaaac
     7141 cgcggaaact acagcggagg aactggagaa accagattcc ggataaccct gggaggcaat
     7201 gaatcggttg aacaccggct gacggccaac cgcgtcaaca tcgttctgga tgcggccatg
     7261 tccaaacacg atgttgtgcc ggggaaaaac cataaagtcc ttgtgcaaat caaaggcccc
     7321 gcggacatga aaagatacga ggtgcgctgg tcggcaagcg gcggcgcctg gcggaaagaa
     7381 accacggatt tttcccttgc ggggcaggaa tggcaatcgg agggagcatt tttaacgccc
     7441 tttgccgcag ataatgcccg tcaaaaaggg caaccggtta aaattgcctt gcaaatcgtc
     7501 gacaaaaaag gcggcaaagc gctttatacc tatgaaaaca agctggcgac cacggatgcg
     7561 gccatcagcg gtcttgagct ccacgccggt ttgaataaaa gatcagcccg caaagttgac
     7621 gaacccattg atctgatcct tggttcaacc gatccaatgg ttctgttatc acctcaggtg
     7681 gttctgccgg acggcgaagt ctttcctctg aacctgaaag cgccctcccg cctggtgaaa
     7741 attgactcca gcaatcccga agtcgcgtct ttgcgcagtg aacgatacgg accggactct
     7801 cttgtaaagg agattatcgt ggcccggcca acgggacgtc tgggcacaac tcaaattacc
     7861 gcccggatgg gaggcagcga ttacgataag gcaggcagtg cttatctggc gggaaatgag
     7921 gaagaaatca aatccaacac ggtcaacgtc agcgtcaaca aggcggttct gattgccgac
     7981 catcaggcgg gcggccgcac tgcttatcgg ctgatcgtca ccggcccggc ggatatgagc
     8041 aaatacctgg ccatctggag gggcgaggcc agcaggagca cacggtttgc tccctgcaac
     8101 aatggatata caagcgatct ggaaacaact cttacaatgg agcaggtctc cgtggtaaaa
     8161 aacaataccg tggtcgcctc gttcaacatc aagtcgggcg cgcgcaaagt caacattaag
     8221 ctccttcccg tcacgccgcc cttcacggtt cttgagaagg ttgcgctcag cgacgcaggc
     8281 agtctggaat ccataacgga ctgcaagaaa aaaatcactt cgatgatcga tcttttcggc
     8341 ttcaaccccg gcatgcccgt ggaagactac tgccgttcgc aaagagagca ggaaaagcaa
     8401 aaaatcaaga ccgaaaggga agatcagaaa accttcaacg aaatgatccg gaatctggac
     8461 cggcaggggc aggagttggt ggtggtcgcg gacagcgtta aaatcggcgc ggccgtgaaa
     8521 ggggatctgg cggcgctgga cagcgcgccc ttttgttcgt ggcgtctgga acgggccgga
     8581 gcaattacgc ttgccgaagc cgtcactccg gtggaaacca tctccgccaa cgagggaggc
     8641 tgcttcaaca atctggtcgg ccttaaaaac gggttcaacc cggatatgac gctgcgcgtg
     8701 gagctgatcc tccttgccaa cccccaaaca ccgctggtga tcaccggcga atccatcaca
     8761 gcggcgggcg gcattttata tcgataacgg agaaaaaaat gaaaataaga tttgacataa
     8821 cgcattcatt tctgactttg tttttcatct tctgggccgc agttaacggc tgggctcaat
     8881 cgacggttct cgatgcggac agccagcagc cctttgccgg ttttggacag tattttgttg
     8941 ctccacaaga accggaaaag caaaaagaaa cattgccttc ttcaccctcc gcagccgaag
     9001 gtgaagcggt tttcaaaatt ctcagcataa ccaacatcag cggcaataat cccttccata
     9061 cgacacagcc ggttctcaat tttggcggtg tggtcgtctc cagcgtggac gaccttgcgg
     9121 aatgcatccg cggcggcgtc tgccaatccg gcaaaaaaga aacaactatg acctgttcgc
     9181 cgtcttcagg aatatccgat ctggcttctt ggatagaaag cgtcggctgc tatttaaaga
     9241 gcaacgccac ggtttgccgg gttgagccgc tgccgtcatc gccggatttg agctatgaaa
     9301 cttttttcgt catgaatgac tatagatttg ccaaaagcaa tgtcgtggga accgcaagca
     9361 aatcggacta caagctgatc atcccgccgt ctgggaatac gtatgtctgg ccgggcggtg
     9421 ataccgtcta taaaatcgga acgggaactt acacgctgga agtgcagcat aaagtgaccg
     9481 gtttgcgcta ccgcatcgag tttaccgtgc agcaactggc cagcaacgat atccgtttaa
     9541 ccgtaaattc tcttgtcaag ctatgaaatt aacggttttg cgtggaggga gaatgagacc
     9601 aaataagaac aagcgggcag aatcaaactg ttcgatcgcc gtgattcttt tgctgttttt
     9661 tttcgtggca gcctcacccc tatgcgcgga agatcctgaa acaccgggag aatggatcaa
     9721 aacctctttt gatgcctgga agatcaacgg aagcaatacc ctcagagtgg attattatgg
     9781 aaattcggga gatgaaaccc aggccactta ccccttcacc gggttgagat cctacgacga
     9841 gctcaatctc aacttcagca aatcggtcag caaatacgac acctggcggg gcgaaattac
     9901 cggcgtgctc aacaactcgc cttaccggag caactactac ggaattgttc ccgagcgact
     9961 gaatctgacc aaaaccaagg gtgacagtct catccccctg cgtctggaaa tgggggacat
    10021 tttctcttat tacacgtacc gtaccttaca gagctccctg aagggagtgc agcttgatct
    10081 tcagccattc caaaactccg gcagcagaca ttctttcatc atcacctcag gcgctcggca
    10141 atcttcctgg cgcaatttcc accttcagga cagctacttt aacggcgctt cgtatatgat
    10201 agacaacgca aaattcggca aattcagctt aaacgccgtt caaaacagca ggcaggctga
    10261 tgaacaacaa gcctctctga gccgttcgca gttgatttat agcgccgccg gaaatgcaat
    10321 tgtgaaagcc gtcaatcaaa aactgcttct ggaaggcgag ttgtccaggt tcaccgggga
    10381 tcacgatggc tgggacgggc cggaaagcgg acagaaacaa agtgacaatg gattgttttt
    10441 tcaaggttcc ggttcgagca actggccact gacgtatcgg gtccgctatg agtcttacgg
    10501 ccgggattac cgaccctcgg cagcttcagt ttctccggcg acaaaaaatg cggaaggaca
    10561 cctgggctgg cgtttgccca agggttatca gatgagggcc cgcgcccaga agaccaccag
    10621 caactacgaa atgaataacc cgacggacac gattgtttac ggcttcaact taaacggccc
    10681 gctgggatct ctggacgctt tcggacagga cattagcagt gatgacaaag ccaccgaccg
    10741 gatcaccggc acagtgagcg ccaacattaa tattccagcc atcatcggat ggaaaagccg
    10801 gctcggctgc cagattcagg acctttccga ccgcattgcg ggaggcgtcg acgtcaaaac
    10861 ccgtcaaatc agtttcagcg cggaccgctc tgtgcatttc ggcagtttct ccggctccgt
    10921 cacccccggc attctctatc gccaaacaga aagcccgacc gccaaaacgg atgaactcct
    10981 tccgacgctg attgtggggt taaaaagcgg cccccacagc ctggactaca acctttcctg
    11041 gtcggacctg cagcggcctt acgatggagg cgtcgatgtg ggaacagtca accagggctt
    11101 caattatcga tacaccacgg aacgctccgt tctgggagtg gagatccttt acggccagag
    11161 agatccggat cccgggcaga agacgggcag ctacaaattg ggcgcctact ggacctttta
    11221 tttcgacaaa cctgccaaaa ccaccacggg aaggaaacca aaaccagagg agcaagcgct
    11281 tgtgcaagac agtgaaagcg ccattcccgc tctgttcaat gtagatatag ccgcactgat
    11341 ccccggcatg aaaatcgaaa aagccgttga actgcttaag gcgaaaggca ttgtaaaccc
    11401 cgtccgcgat gatgtttatc tgacctatga aaccagagtc ctggaagaaa ttgaacagcg
    11461 tcagcgcctt gtcctcatcc aggaaaataa aatcctgaaa aaagctctcg tcattattga
    11521 ttttggcgcc atcgaccgtc cagaggacat gctccagacc tatcagctgg ttcaatccat
    11581 cctgcttgac aaatacggca acccgaccag cgtaaccagc cgcggcgagc tcacacaaac
    11641 cttttttcaa gatgtccgcg atggaaagtt tgtccgctca aacgattggg aaaggcccgg
    11701 cggaaaactc cgcttctgca ttcccaagcg tctggacgga atggtgcgtt tgagcatcga
    11761 gtttgcggtt agttttcctc ccccggcaga gatgtgggat gacgcaacct tccgttagtc
    11821 atacaagcga caacgattcc atcaaaagct ctgtgcgcct gggctgttgt ccttccattt
    11881 gactaaaaga ttcttttccg ttattgaaag aatccttatt gcaaataaac aattcaggag
    11941 aatttgacca tgtctaatat actgcttgtc ggaaacggcg cgcgcgaaca tgcgatagcc
    12001 gaagccattg cgcgatcccc gcaaaacccg cgtttattct ccttcatgaa agccaacaat
    12061 ccgggaattg cttccctgtc ggaaaaaatt caacccggca gctatgcgga tcttgcagcg
    12121 atcacaggct tcgcgacggc aaacgaaatt gaatttgccg tgatcggtcc ggaagacccg
    12181 cttaacaacg gcgtcgtgga tgcactggcg caaatcggaa tcccggccgt cggccccaat
    12241 caaagcctgg cgcggctgga aacttccaaa tcgttcaccc gcaatctggt gagcaaatac
    12301 aatatccccg gcaatccgca gtttaaagtc tttacatcca tggacggcgt cgaagcattt
    12361 ttaaatgctt tggaaggcat cgtcttgaaa ccggacggat tgaccggcgg caaaggcgta
    12421 ctggtacagg gcgatcattt tgccacaaaa gaagaagcgc tcaaattatg cactcagatt
    12481 ttaaaagaca gtttctctgt tattgttgaa gaaaaatttg acggcgaaga attctcgctg
    12541 cagtgcctct gcgacggaaa aaccgttgtc ggcacaccgc tggtgcagga ccacaaaaga
    12601 cgctttgacg gcgaccgcgg ccccaatacc gggggcatgg gctcctattc ccttcccgac
    12661 cattccatgc cttttctaaa accgtcggac atcgcagaag ggctggaaat cacccggcag
    12721 gtggcggcgg cgcttttaaa agaaaccggc aacccttaca agggggtcat gtacggcgga
    12781 ttcatcgcca cgaaaaatgg tgcgaagctt ctggaataca acgcgcgctt cggcgatccc
    12841 gaggccatga acattttgcc gcttttaaaa acggacttcc tggaggtctg ccgtcacatc
    12901 attgacggca cgctcgacaa attgaaaatt gaatttgagc cgaaagcaac cgtctgcaaa
    12961 tacattgtcc cgaaaggcta tggcctcccc gccgatcatc cggatgcgtc atcgtcgaaa
    13021 gcgaagatag ccgtgggcga cgtgggtgcg gccagacttt actattcatc cgtggataaa
    13081 agagaggacg gcttgtatct gagttcgtcg cgcgccatcg gcattgtcgg cattgccgat
    13141 acgctggacg ccgcgcgcc
//
LOCUS       DAOSIM010000034        12936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_4889_length_12936_cov_6.008074, whole genome shotgun sequence.
ACCESSION   DAOSIM010000034 DAOSIM010000000
VERSION     DAOSIM010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 12936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12936
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4889_length_12936_cov_6.008074"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PLR47_02785"
     CDS             complement(<1..307)
                     /locus_tag="PLR47_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11893.1"
                     /translation="MEKTITKVYVDNEGFVVFVCSKCNLIQKQEAKTYGGAKGPIQVQ
                     CTCGNVYDVEIEFRKFYRKGIRVDGLYFGVTNPKIWGKMVLKNVSMQGCGFETMGANP
                     "
     gene            complement(427..642)
                     /locus_tag="PLR47_02790"
     CDS             complement(427..642)
                     /locus_tag="PLR47_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOG11894.1"
                     /translation="MSMDITAPMPGKIASIAVNVGTQVQEEEEVIIMDAMKMEIPVYA
                     PGAGTIKEIKVKVGDSVNEGQVLAVLE"
     gene            complement(798..2027)
                     /locus_tag="PLR47_02795"
     CDS             complement(798..2027)
                     /locus_tag="PLR47_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG11895.1"
                     /translation="MINFLSTAKISLRALRANKMRSALTMLGIIIGVAAVITMLAVGR
                     GASNKISEQIATVGSNLIIVLPGSTTASGIRLGSGAFQNLTRADAEAIRQECSAVATT
                     APILNGSAQIIYGNANWPSVVFGTDENMLEVRDWTLSAGRNFYDQEIRSAAKVCLLGQ
                     TVVDNLFGNDDPLHKTVRIKKVPFVVIGVLAPKGQSMMGQDQDDIVYAPVSAAQRNLF
                     GRSFPGTVRSIMVKAKSTEDLDRAEQQIKELLRQRHRIGAGQEDDFTVRNLTQMMQMA
                     EQASRVMALLLAAIAGVSLLVGGIGIMNIMLVSVTERTREIGIRMAVGAKTWDIRLQF
                     IIEAMILSLIGGLIGVLLGILFAELLSFFAGWTILISTYSILLSFGFSGLVGIFFGFY
                     PAYKASLMNPIDALRHE"
     gene            complement(2024..2713)
                     /locus_tag="PLR47_02800"
     CDS             complement(2024..2713)
                     /locus_tag="PLR47_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG11896.1"
                     /translation="MALIETDHLEKRYEVGDSIVHALDRVSLQIHQGEFVAVMGPSGS
                     GKSTFMNVIGCLDVPTAGTYRLDGIDVGSMSRDALAEIRNEKIGFVFQGFNLLARTTA
                     LENVEVPLLYNKDGSKNRRERALDALRTLGLEDRRHHHPNQLSGGQQQRVAIARALVN
                     NAPLVLADEPTGNLDTKTSIEIMELFVRLNEEKNITIVLVTHEPDISAYSKRIIHFLD
                     GKLVSDSVNKR"
     gene            complement(2719..>3734)
                     /locus_tag="PLR47_02805"
     CDS             complement(2719..>3734)
                     /locus_tag="PLR47_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG11897.1"
                     /translation="NAVTTVLVGTQVSGTIKELYVDYNSTVRQGQLLARIDPASIEAQ
                     VAQAAANLSLARANLEKSKVALRDTVSVFERNKILYGKNFISRHDLDTSETAYLSALA
                     QIKASEAQVEQTRAALNLAETNLRYTRIVSPVKGTVISRSVDVGQTVAASFQTPTLFT
                     IAQDLTKMQIVANIDEADIGRLRVGQPVTFTVDAYPDLTFGGNVSEIRNSPTTVQNVV
                     TYDVIVKVDNSDLKLKPGMTANVSIVIDDKKGILRVPNAALRLKIQDKSSSAPAPKGP
                     GVWVLENKKPKRVPLSIGISDNRFTEVVSGKILQGAPVIVEVKSNDQKNAATQTPRPG
                     PRF"
     assembly_gap    3735..3744
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3873..5153)
                     /locus_tag="PLR47_02810"
     CDS             complement(3873..5153)
                     /locus_tag="PLR47_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG11898.1"
                     /translation="MRKRIFLLFLLLFIPAQSLFAAGDIQKGDVLTLRQCIDIALKNH
                     PALNAAAGAIHQSESKIGKARAGYYPQLSFQSGYSRIGPPASSLTNSSYDYYSNALNL
                     NQTLFDFGKTSTAVDIQKLGKQSAEADFQDTTSLLTYNVQEAYFSLLKAKMSETVAVE
                     TVNQFQQHFEVAQTFFETGKSSKIDVTSAEVNLSNARIQLISAQNALRLWRVNLNKAM
                     GVINAPEYDIQEKFNMDAADITFEAALARAYENRPDIQSIALKKDALERTVDLNKKDY
                     LPVLSGSAGYGYAGQDAALNQSWNVGVALTFPFFTGFSTKYAVEEARAGLEIAKANEE
                     ALRQKAFQEVKSAWLNRHEAFERIEAGKITVRQAEESLELAQGRYATGVGNSLEIVDA
                     MIKLNNAKMTYISALSDFNIAEASLEKAMGAKMP"
     gene            5659..6531
                     /locus_tag="PLR47_02815"
     CDS             5659..6531
                     /locus_tag="PLR47_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOG11899.1"
                     /translation="MRIFVRKWSALVVLLCLVLVAGCASLSSQKSDQFIFLSSTIGPI
                     DSGIVGALEDQFEKETGIRVRHVGAGTGAALKLATKGQIDLVMAHAKSLEENFIADGY
                     GTERIPLMYNDFVIVGPASDPAAVKGEKSATAALKKIMDANGKFISRGDKSGTHVAEM
                     ELWKKTGVEPKGAWYVIYEKGSEGNVATLKYTDQQAAYTVIDRATFLSLKDQIKVVIL
                     VEGDEALLNYISLIPVNPAKFPRVNRKDTLTFVQWLTSPEKGQLIIRDFGKEKYGSPL
                     FFPNSVEWQKLQKK"
     gene            6556..7422
                     /locus_tag="PLR47_02820"
     CDS             6556..7422
                     /locus_tag="PLR47_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOG11900.1"
                     /translation="MKMFKSSKWFIILAVVAALVMLGAAGAFAAPKQKNIILATTTST
                     QDSGLLDTLIPIFEKETGYFVKTIAVGSGQAMKMGEKGEADVMLVHSPDAEKKFVEAG
                     YGINRRLVMHNDFIIVGPPNDPAGIKGVKSSSDALKKIAGANALFISRGDNSGTHAKE
                     KTLWKKANVNPAGQKWFQETGLGMGQTLNVASEKKAYTLADRGTFLAIRKNLGLTILN
                     EGDAALLNIYHVIEVNGAKWPKANTEGAKAFADFMVSKKTQSIIKTFGVDKYGSPLFF
                     PDAGKKVEDLGK"
     gene            7431..8129
                     /locus_tag="PLR47_02825"
     CDS             7431..8129
                     /locus_tag="PLR47_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG11901.1"
                     /translation="MDLIVQGVIKAFQLIISFDPEVMGITWLSLKISGTATFISLFFG
                     VSVGTAVALNNFPGKRLVISIINTGMGLPPVVVGLFVTIMIWRSGPLGFLEILYTPYA
                     MIIAQAIIATPIVMGISLASIQNLPPNLRLQILSLGASRMQMIWVLIKESRLPLLAAV
                     MAGFGGVISEVGASIMVGGNIKGYSRVLTTATVMETGKGNFDIAIALGIILLILAFLV
                     NLLLTQIQQRQHPR"
     gene            8126..9211
                     /locus_tag="PLR47_02830"
     CDS             8126..9211
                     /locus_tag="PLR47_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG11902.1"
                     /translation="MNNIFTIENLIVRRGGVKVLDINELKVHESRILALIGPNGAGKS
                     TLLLTMAGLLKPDQGKMHYKGRPVESSAERSRMRRSVCVVFQEPLLLSTSVYKNVALG
                     LKFRGMSKNDIQKNVGDALSYFGISSLANRSAKTLSGGESKRVSLARAFALKPHLILL
                     DEAFNSLDPPTRESLIDDLRHILEETKITAVLALHDREETLRLAQDVTVMSEGRLIQT
                     GATAQIFNQPQSEFVANFVGTETILEGTVQNIRSGLMEVLVSGKVIEAAGAAPLGQKV
                     FCCIRPENIVVSREAPENTSARNIYEARIQKIVRQAFSYKLVLDCGFPVVAYLTVSSC
                     EDLGFCEGAAVFASFKATAVHVMRREK"
     gene            complement(9298..10296)
                     /locus_tag="PLR47_02835"
     CDS             complement(9298..10296)
                     /locus_tag="PLR47_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HOG11903.1"
                     /translation="MALFEGYERRIKQITPFLAKYGISSLEEAEKVCIGKGVDVRKIV
                     KDIQPISFENAGWAYLVGAAAAIKKGQKNAADIAETLGEGLQSFCIPGSVADDRQVGI
                     GHGNLASMLLREETACFAFCAGHESFAAAEGAIGLAKSANKARKNPLRVILNGLGKDA
                     AHIIARVNGFTHVATAFDYKTGKVSVAREKAYSKGDRAAVRCYGADDVREGVAIMHLE
                     GVDVAITGNSTNPTRFQHPVMGVYKKERLLAGKKFFSVASGGGTGRTLHPDNMAAGPA
                     SYGMTDTMGRMHSDAQFAGSSSVPAHVEMMGLIGMGNNPMVGASVAVAVAVEQAMK"
     gene            complement(10308..11000)
                     /locus_tag="PLR47_02840"
     CDS             complement(10308..11000)
                     /locus_tag="PLR47_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11904.1"
                     /translation="MEHTHEIEQMCVVAKGAKNGPAPIPQEGGWTPVKEIKDISGLTH
                     GVGRCAPQQGACKLTLNVKKGIIEEALVECIGCTGMTHSAAMAAEILPNKTILEALNS
                     DLVCDAINVAMREIFLQIVYGRTQTAFSEGGLPIGAGLEDLGKGLRSVIATMYGTKVK
                     GPRYLEMAEGYVTEIGLDADDEIIGYKFVQLGVMMDNITKGMDPAAALSKASGTYGRF
                     AEAVKVINPRKG"
     gene            11327..11752
                     /locus_tag="PLR47_02845"
     CDS             11327..11752
                     /locus_tag="PLR47_02845"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOG11905.1"
                     /translation="MNYQLYLEKIRAGEKGLNPYLDHFNVEVAGLSSGRAVFRMPIRP
                     EYMQGAGLMQGGLMVALADEAIAHAMMTVLTPEEGLTTIELKSNFLAGVDKGELIAEA
                     GVFKKGRSLIIGDCQVTNGAGKPVCRVSATFMLLRQHQK"
     gene            complement(11810..>12936)
                     /locus_tag="PLR47_02850"
     CDS             complement(11810..>12936)
                     /locus_tag="PLR47_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOG11906.1"
                     /translation="TIDIGLCATPMLYFSIRRLGAGGGVMITGSHNPPEFNGFKICIG
                     YDSIHGEQIQELRRIMESGSYHSGKGSAQAQDITKIYQDYIFDSVRVSKKLKIVLDGG
                     NGVGGKFALPILERMGCDVTALYCEPDGRFPHHFPDPTVEENLAELIRLTAKEKADLG
                     IAFDGDADRIGVISDTGEIIWGDKLLLLFARFILKDHPGATIIGEVKCSQVLYDGIAE
                     YSGRPLMWKAGHSLIKAKMKEEKALLGGEMSGHLFFADRYFGYDDAIYAAVRLLEILS
                     QTGLKLSELLAGVPKTFATPEIRMDCDDDRKKAVVAKIKEHYQNTPGVIDIDGVRVPF
                     GDGWALVRCSNTQPVIVLRFEASSAARLQEIRGEVEGLLA"
BASE COUNT         2743 a         3493 c         3715 g         2975 t
ORIGIN      
        1 acgggttggc gcccatcgtt tcaaagccgc atccctgcat cgagacgttt ttcaaaacca
       61 ttttccccca gattttgggg ttggtcactc cgaagtacag gccgtccacc cggattccct
      121 tgcggtagaa tttccgaaac tcgatttcca cgtcatagac gtttccgcag gtgcattgaa
      181 cttgaatcgg gcctttggcg cctccatagg tttttgcttc ctgcttttgt atcaaattgc
      241 atttggagca gacgaacaca acaaatcctt cgttgtccac gtacactttc gtaatggttt
      301 tttccatacg ccatcccctc atcggtttct tgacttaggt aagcacagta taaaatggcc
      361 cgaaaagaaa aaaacccgtc ttgcgattct gaacatcgca aaacgggttc catcgtccaa
      421 atcgatttat tccagaacgg ccagcacctg gccttcattg acggagtctc ccaccttgac
      481 tttgatttcc ttgatggttc cagcgcccgg ggcatacacg gggatctcca ttttcatggc
      541 gtccatgatg atcacttcct cttcttcctg cacctgcgtg cccacgttca ccgcgatgct
      601 ggcgattttc cccggcatgg gggccgtaat atccatactc ataatttttc cccgcctccc
      661 tttatcgata ttttttgtct cacaaaatga acgccaaagc ctgcgccgtc caagccgccg
      721 ctttgaaatt gttgaagctt ttaccgtaaa tgcgggttca ggtcaataca attattcggc
      781 ggcgggcgcg tgaccgctca ttcatgccgc agagcgtcga tggggttcat caaagaggcc
      841 ttgtaggccg gatagaagcc gaaaaagatg ccgaccaggc cggagaatcc aaaggagaga
      901 aggatggagt aggttgaaat caggattgtc cagccggcga aaaaggatag aagctccgcg
      961 aacagaatgc ccagcaaaac gccgatcaat ccgccgatga gcgagagaat catcgcctcg
     1021 atgatgaatt gcaggcggat gtcccaggtt ttggcgccga cggccatgcg gatgccgatt
     1081 tccctggtgc gctccgtgac cgaaaccagc atgatgttca tgatgccgat gccgcccacc
     1141 agaagcgaaa caccggcgat ggcggccagc agaagcgcca tgacgcggga agcctgctcc
     1201 gccatctgca tcatttgcgt caggttgcgg acggtgaagt cgtcttcctg gcccgcgccg
     1261 atgcggtgcc gctgccgcag cagttccttg atttgctgtt ccgcccggtc cagatcttcc
     1321 gtgctcttag ccttgaccat gatggagcgc accgtgcccg gaaacgacct tccgaaaagg
     1381 tttctctgcg cggcggagac cggcgcatag acgatatcgt cctggtcctg gcccatcatg
     1441 gactggcctt tcggggccag aacgccgatg acgacaaacg ggactttttt gatgcggacc
     1501 gttttgtgga gcgggtcgtc gttgccgaat agattgtcca cgacggtttg tcccagcaga
     1561 cagactttgg ccgcgctgcg gatttcctga tcgtaaaagt tccggccggc cgacagggtc
     1621 cagtcccgga cttccagcat gttttcatcc gttccgaaga cgacgctcgg ccagttggcg
     1681 ttgccgtaga tgatctgggc gctgccgttg aggatcgggg cggttgtggc caccgcggag
     1741 cattcctgcc ggatcgcttc ggcatccgcg cgggtcaggt tctgaaaggc gccgctgccc
     1801 aggcgaatgc cgcttgcggt ggtcgagccg ggaaggacga tgatcaggtt gctgcccacg
     1861 gtggcaatct gctcggatat tttattgctg gcgccccggc cgaccgccag catcgtgatg
     1921 acggcggcca cgccgatgat gatgcccagc atggtcagcg ccgaacgcat cttgttggcg
     1981 cgcagggcgc gcagagaaat tttagcggtg gaaagaaagt tgatcatcgt ttgtttaccg
     2041 agtcgctgac cagttttccg tccaggaaat ggatgatgcg cttgctgtag gcggaaatgt
     2101 ccggttcatg cgtgaccagg acaatggtga tatttttctc ttcgttgagc cggacgaaga
     2161 gctccatgat ttcgatgctg gttttcgtgt ccaggttgcc ggtcggttcg tcggcaagga
     2221 cgagcggcgc gttgttgacc agcgcgcggg cgatggccac ccgctgctgc tgaccgccgg
     2281 aaagctggtt ggggtggtgg tggcgcctgt cttccaggcc cagtgtccgg agcgcatcca
     2341 atgcccgctc cctgcggttt ttgcttccgt ctttgttgta gagcaggggg acttcgacgt
     2401 tttccagcgc cgtcgtgcgc gcgagcaggt tgaatccctg aaagacaaat ccgatttttt
     2461 cattgcggat ttccgcgagc gcgtcgcggc tcatagaacc cacgtcgatg ccgtcgaggc
     2521 ggtacgttcc ggccgtcggc acatccaggc agccgatgac gttcatgaaa gtggatttgc
     2581 cggagcccga gggccccatc accgccacga attctccctg atggatctga agggaaactc
     2641 tgtccagcgc gtgaacgatg ctgtcgccga cttcgtatcg cttttccaga tggtccgtct
     2701 cgatgagcgc catggccgtt agaacctcgg tccgggccgc ggggtctgcg tcgccgcgtt
     2761 tttctggtcg ttgcttttga cttccacgat gaccggcgcg ccctgaagaa tttttccgga
     2821 gacgacttcc gtgaagcggt tgtcgctgat gccgatggaa aggggaacgc gtttgggctt
     2881 tttgttttcc agcacccaga cgccggggcc tttcggcgcc ggagcggagg aggatttgtc
     2941 ctggattttc agcctcagcg cagcgttggg aacgcgcagg attccttttt tgtcgtcaat
     3001 gacgatcgag acgttggccg tcatgcccgg cttgagcttc aaatccgaat tgtccacctt
     3061 gacgatcacg tcataggtga ccacgttctg caccgtggtg ggcgagttgc ggatttcgga
     3121 aacgtttccc ccaaacgtca ggtcggggta ggcgtccacg gtgaaggtga cgggctggcc
     3181 cacgcgcagg cggccgatat ccgcttcgtc gatgttggcc acgatctgca ttttggtcag
     3241 gtcctgggcg atggtaaaca gcgtcggcgt ctgaaagctg gcggcgacgg tttgcccgac
     3301 gtccacgctg cgcgagatga ccgttccttt gaccggcgaa acgattcttg tataacggag
     3361 attcgtctcg gcgaggttca gcgcggcccg ggtctgctcc acctgcgctt cggaggcttt
     3421 gatctgggcg agcgcggaca ggtaggcggt ctccgacgtg tccaggtcgt gtctggaaat
     3481 gaaatttttg ccgtaaagga ttttgttccg ctcaaatacg gaaaccgtgt cgcgcagggc
     3541 gactttggac ttttccaggt tggcgcgggc cagcgacaga ttcgcggcgg cctgggccac
     3601 ctgcgcctcg atcgaagccg gatcgatccg ggccaaaagc tggccctgcc tgaccgtgga
     3661 gttgtaatcc acgtaaagct ccttgatcgt tccggacacc tgcgtgccga ccagaacggt
     3721 cgtgacggca ttgannnnnn nnnncgtcgc gcggatgtcg ccccggtccg ccgtctgggt
     3781 gatatactgc ggcgccggct cgcggcggct taaaaagaaa aacagggcga tcagaatcag
     3841 gattagaatg acgctcgcgg caatgatttt tttcatggca tttttgctcc catggctttc
     3901 tccaggctgg cttcggcgat gttgaaatcg gacagggcgg atatgtaggt cattttggca
     3961 ttgttgagct tgatcatggc atccacgatt tccagggaat tgcccacgcc ggtcgcgtag
     4021 cgcccctgcg caagctccag agattcttcg gcctggcgca cggtgatttt gcccgcctcg
     4081 atccgttcaa aagcctcgtg gcggttgagc caggcggact tgacttcctg aaaagccttc
     4141 tggcgaagcg cttcctcgtt ggctttggca atttccaggc cggcccgggc ctcttcgacg
     4201 gcgtatttgg tggagaagcc cgtgaagaaa ggaaaggtga gggcgacgcc gacgttccag
     4261 gactggttca gggcggcgtc ctgacccgcg tagccgtagc cggcgcttcc ggaaaggacg
     4321 ggcagataat ccttcttgtt gagatcgacg gttctttcca gcgcgtcttt tttcagagcg
     4381 atcgactgga tgtccggccg gttttcatag gcacgggcga gggcggcttc aaacgtgatg
     4441 tccgcggcgt ccatattgaa cttttcctga atgtcgtatt cgggggcgtt aatcacgccc
     4501 atggccttgt tcaggttcac ccgccaaagg cgaagggcgt tctgggcgga aatcaactga
     4561 atgcgggcgt tgctcaaatt gacttccgcg ctggtcacgt cgatcttgga gcttttcccc
     4621 gtttcaaaaa atgtctgggc cacttcaaaa tgctgctgaa actggttgac ggtctccacg
     4681 gcgacggttt cgctcatctt ggccttgaga agggaaaagt aggcttcctg aacattgtag
     4741 gtgagcaaag aggtggtgtc ctgaaaatcc gcttccgccg actgcttgcc cagcttctga
     4801 atgtccacgg cggtggaggt tttgccgaaa tcaaacaggg tctggttcag attcagcgcg
     4861 ttggagtaat agtcgtagga gctgttggtc aaagacgacg cgggcgggcc gatgcgcgag
     4921 tagccggact gaaaggaaag ctggggatag taaccggcgc gggccttgcc gattttgctc
     4981 tccgactggt gaatggctcc ggcggccgcg ttgagggccg gatgattttt cagggcgatg
     5041 tcgatgcact gccggagcgt taggacatct cccttttgaa tgtcacccgc cgcgaacaga
     5101 ctttgggccg ggatgaacaa cagcagaaac agaaggaaaa ttcttttgcg catagaagat
     5161 gtaaaaaacc ctttcaaatc gtaaatccct ctttcccgga tttcatacca caaatcattt
     5221 ctcgttcaca cctatatcaa tttttgtgcc agtaatcatg gtggatagtg aatcgtggat
     5281 ggtggatggt taaatgtagg gaacggtcgc gaccgttccc tacgggtaat aggtcatgct
     5341 tgtatcttcc atcgcttgtg tcatgctgag ccagtcgaaa catgacgctt tccgctcaac
     5401 acgcattgct ttttgccccg cacccatcac ctctaacccc ggccccctta cctctcatcc
     5461 aacaacccgc tgcatcttat ttccccacct ttcgtatcca atcgggtaat ctgttcccat
     5521 taaaaaatct gaattatatt tggccgataa ttggcaataa ggtaattcaa tgtctgattt
     5581 tggaatatta ttcttgatta aattttcaga atgcattacg aaatcaggcg caaaatgaaa
     5641 cgtgaaagga gcatgaatat gaggattttt gtcagaaaat ggtcggcgtt ggttgttttg
     5701 ctttgcctgg ttctggtggc cggatgcgca tcgctgtcgt cccagaaaag cgatcagttc
     5761 atctttttgt ccagtacgat cggcccgatc gattccggca ttgtgggggc gctggaagat
     5821 cagtttgaaa aagagacggg aatccgcgtc cgccacgtcg gcgcggggac cggtgcggcg
     5881 ctcaagctgg ccaccaaggg ccagattgac ctggtgatgg cccatgcaaa atcgctggag
     5941 gagaacttta tcgccgacgg ttacggcacg gagagaatcc cgctcatgta caacgatttt
     6001 gtgattgtcg gccccgcatc cgatccggcc gccgtcaagg gagaaaaatc ggcgacggca
     6061 gccttgaaga aaatcatgga cgcaaacggc aaattcatca gccggggaga caagtcgggc
     6121 acgcatgtgg cggaaatgga actctggaaa aaaacaggtg tcgaacccaa aggcgcatgg
     6181 tatgttattt atgagaaggg gtccgaagga aatgtcgcca cgctgaaata caccgatcag
     6241 caggccgctt acacggtgat cgaccgggcc accttcctgt ccctgaaaga tcagatcaag
     6301 gtggtcattc tggtggaagg cgacgaggcg ctgttgaatt acatttcttt gataccggtc
     6361 aacccggcca aattccccag ggtcaaccgc aaggacaccc tgacgtttgt ccagtggctg
     6421 acgtcgccgg aaaaaggcca gctgatcatt cgggatttcg gcaaagaaaa atacggatcg
     6481 ccgctgtttt tccccaattc cgtggaatgg cagaaactgc agaaaaaata atcatcaatc
     6541 tgaaggagac agaatatgaa aatgttcaaa agcagcaagt ggtttatcat cctggccgtt
     6601 gtcgcggctc ttgtcatgct gggcgcggca ggcgcgtttg cggcacccaa gcagaaaaac
     6661 atcattctgg ccacaacaac cagcacgcag gattccggac tgctcgacac cctgatcccc
     6721 atctttgaaa aggagacggg ctacttcgtg aagacgatcg ccgtcggttc cggacaggcg
     6781 atgaagatgg gagaaaaagg cgaggccgac gtcatgctgg ttcactcacc ggatgccgag
     6841 aagaaatttg tcgaagcggg ctatggaatc aaccgccgcc tcgtaatgca caatgacttc
     6901 attatcgtgg gcccgccgaa cgatccggcc gggatcaaag gcgtcaaatc ctcgtcggat
     6961 gcgctcaaga agattgccgg cgccaatgct ctgttcatct cgcgcgggga caactccgga
     7021 acgcacgcga aagaaaaaac cctgtggaaa aaggcaaacg tcaatccggc cggacagaaa
     7081 tggtttcagg aaacgggtct gggcatgggg cagacgctga atgtcgcctc cgagaaaaaa
     7141 gcttacacgc tggcggaccg gggcacattc ctggctatcc ggaaaaacct gggcctcacg
     7201 atcctgaatg aaggcgacgc ggcgcttctg aatatttatc atgtgattga agtcaacggt
     7261 gcaaagtggc cgaaagccaa cacggaaggc gccaaagcgt ttgcggattt tatggtgtcc
     7321 aagaaaacgc agtctatcat taaaacgttt ggcgtcgata aatacggttc gccgctgttc
     7381 ttcccggatg ccggcaaaaa agtggaagat ttagggaaat agcgttcaca atggatttga
     7441 ttgttcaagg cgtcatcaaa gcctttcaat tgatcatcag ttttgatcct gaagtgatgg
     7501 gaatcacgtg gctgtccctg aaaatatcgg ggacggccac tttcatcagc ctgttcttcg
     7561 gcgtttccgt cgggacggcc gtcgcgttga acaactttcc cggaaaaagg ctggtgatca
     7621 gcattatcaa cacaggcatg gggttgccgc ccgtggtggt gggccttttt gtgaccatca
     7681 tgatctggcg cagcgggcct ctgggttttc tggaaatcct ctacactccg tacgcgatga
     7741 tcattgcgca ggccatcatt gcaacgccga tcgtcatggg catttcgctc gcctcgattc
     7801 agaatctgcc gccgaatctg cggctgcaga ttttgtcgct gggcgccagc cgaatgcaga
     7861 tgatctgggt gctgatcaag gaatcccggc ttccgcttct ggcggccgtc atggcgggtt
     7921 ttggaggggt aatttccgaa gtcggcgctt cgatcatggt cggcggcaat atcaagggat
     7981 attctcgcgt gctcaccacc gcaacggtga tggaaaccgg aaaggggaat tttgacattg
     8041 ccatcgctct gggcattatc cttctgatcc tggcatttct ggtcaatctt ctgctcacgc
     8101 aaattcagca aaggcagcat ccgagatgaa caacattttt accatagaaa atttaattgt
     8161 ccgccgcggc ggcgtcaaag tgctggatat caacgagctg aaggttcacg aaagcagaat
     8221 tctggccctg atcggaccca acggcgcggg caaatccacg cttttgctga ccatggcggg
     8281 cctgctcaaa ccggatcagg ggaaaatgca ttacaaggga cggcccgtgg agtcgagcgc
     8341 ggagcgctcc cgcatgcgca ggagcgtgtg cgttgttttc caggagccgc tgctgctcag
     8401 cacgtccgtt tataaaaacg tggctctggg gctgaagttc cgcggaatgt cgaaaaatga
     8461 cattcagaaa aacgtcggag atgcgctgtc gtacttcggc atcagttcac tggcgaaccg
     8521 gtcggccaaa acgctttcgg gcggcgagtc caaaagggtg tctctggcgc gcgcctttgc
     8581 cctcaaaccg catttaatcc tgctggacga ggccttcaac tccctcgatc cgccgacgcg
     8641 cgagtcgctg atcgatgatc tgcggcatat tctggaagag acgaagatca ccgccgtgct
     8701 ggcgctgcac gaccgggagg agacgctgcg gctggcgcag gatgtcacgg tgatgtctga
     8761 aggacggctc atccagaccg gcgccacggc tcaaatcttc aatcagcccc agtccgagtt
     8821 cgtggccaat tttgtcggca ccgaaaccat tctggagggg accgtccaaa acatccggtc
     8881 gggtctgatg gaagtgctcg tgtcgggaaa agtgatcgag gcggcaggcg cagctccgct
     8941 gggacagaaa gtattttgct gcatccggcc ggaaaacatc gtcgtatcaa gggaagcgcc
     9001 ggaaaatacg agcgcccgaa acatctatga ggccagaatt cagaagattg tccgccaggc
     9061 cttttcctac aaactggtcc tggactgcgg atttcccgtg gttgcctatc ttaccgtttc
     9121 ttcctgcgag gatctggggt tctgcgaagg cgccgcggtt ttcgcgtcgt tcaaggccac
     9181 ggccgttcac gtcatgcgcc gggaaaaata gaccggcgat gcagctgaaa aaaagcggcc
     9241 gcagccgccc ttgcgggcgg catgaaaccg ctcttcatcc attttgaaaa atcccgatta
     9301 tttcatggcc tgttcaaccg cgacggcgac ggcgaccgaa gcgccgacca tcggattgtt
     9361 gcccattccg atcaagccca tcatctccac gtgcgcggga acggaggacg aaccggcgaa
     9421 ctgcgcgtcg gagtgcatgc gccccatcgt gtcggtcatg ccgtaggaag cgggaccggc
     9481 cgccatgttg tcgggatgaa gtgttcttcc cgttccgccg ccggaggcga cggagaaaaa
     9541 cttctttccc gccagcaggc gttctttttt gtaaacgccc atcaccggat gctggaagcg
     9601 cgtcggattc gtggaattcc ccgtgatggc cacgtccacg ccttcgagat gcatgatggc
     9661 cacgccttcg cgcacgtcat cggcgccgta gcagcggacg gccgcgcgat cgcctttgga
     9721 ataagctttt tccctggcca cggagacttt ccctgtttta taatcgaaag ccgtcgcgac
     9781 atgggtaaat ccgttgacgc gggcgataat gtgcgccgca tccttgccca ggccgttcag
     9841 aatgacgcgc agaggattct tgcgagcctt gttggcggac ttggccaggc cgatggcgcc
     9901 ttccgccgcg gcgaatgatt cgtgtcccgc gcagaaggcg aagcaggcgg tttcttcgcg
     9961 cagcagcatg gaggctaaat tcccgtggcc gatgcccacc tgacggtcgt cggcgacgga
    10021 tccgggaatg cagaaggact gaagtccctc gcccagcgtc tcggcgatgt ccgcggcgtt
    10081 tttctggccc tttttaatcg cggctgccgc gcccacaaga tacgcccatc cggcgttttc
    10141 aaatgaaatc ggctgaatat cttttacaat tttccgaaca tccacgcctt ttccgatgca
    10201 cactttttcg gcttcctcca gagaggaaat tccatatttg gcgagaaaag gagtgatctg
    10261 ttttattctt ctttcgtatc cttcaaatag tgccatttta aaactcctta tcctttacgt
    10321 gggttgatga ctttaacggc ttcggcaaag cgtccatacg ttccgcttgc cttagacagc
    10381 gcggcggccg gatccatccc tttggtaatg ttatccatca tgacgcccag ctgaacgaat
    10441 ttgtagccga tgatttcgtc gtcggcgtcg aggccgattt cggtcacata accttccgcc
    10501 atctccagat agcggggccc ttttactttg gtgccgtaca tggtggcaat tacgcttcgg
    10561 aggcccttgc ccagatcttc cagacccgcg ccgatgggaa gtccgccttc ggaaaacgcg
    10621 gtttgcgtgc ggccgtaaac aatctgcaaa aagatttccc gcatggccac gttgatcgcg
    10681 tcgcagacga ggtcggaatt cagcgcttcc agaatggttt tgttgggcag aatttcggcg
    10741 gccatcgcgg cggaatgggt catgccggtg cagccgatgc attcaaccag cgcctcttca
    10801 atgatgcctt ttttcacatt cagggtcagt ttacaggctc cctgctgcgg cgcgcatcgc
    10861 ccgacaccat gcgtcaggcc gctgatgtcc ttaatttcct tcacgggcgt ccagccgccc
    10921 tcctgcggta tgggcgcggg gccgttttta gcgcccttgg cgactacgca catctgttca
    10981 atttcgtgag tgtgctccat aaaaactcca tctccttccg gtaaaatatt ggggataacg
    11041 gttggttatc ctgtttttct tcgcactgaa cgggaaaagt ccctttacgg gtggttcaga
    11101 agccgtcaag acgggaagaa tctattcaaa aagaaaatat tagtcaatat gattattgac
    11161 atggcgccgg atatttttgt ccggacctga tttttttgag ccctccgaat ctcatcgcgc
    11221 aaaggcataa gcaggcggaa aaacattggc aatttcccca aacaggaatt ataagtcttc
    11281 aggattgatg cgtcatggcc gaaaataaaa aaagggaggg gctatcgtga attaccaact
    11341 ttacctggaa aaaattcgcg cgggagagaa agggttgaat ccctatctgg accacttcaa
    11401 tgtagaggtt gccggtttgt cttcgggccg cgccgtattt cgaatgccca tacgtccgga
    11461 atatatgcag ggcgccggat tgatgcaggg aggtttgatg gtcgcgctgg cggacgaggc
    11521 cattgcccat gccatgatga cggtgctcac cccggaagaa ggactgacga caattgagct
    11581 gaagagcaat ttcctggccg gcgtggacaa aggagagctt attgccgaag cgggcgtttt
    11641 caagaagggc cgaagcctga tcatcggaga ttgccaggtg acaaacggcg cggggaaacc
    11701 cgtctgccgc gtttccgcga cgttcatgct gctgaggcag catcagaagt gaggcgggaa
    11761 attatatgca atgcccgtca gttttcggga caccgcggtc cgggactgat caggcgagaa
    11821 gcccttcgac ttcgccgcga atctcctgaa ggcgtgccgc ggatgatgcc tcaaagcgaa
    11881 ggacgatgac cggctgcgtg ttggagcagc ggaccagcgc ccagccgtcc ccgaaaggca
    11941 cccgaacgcc gtcaatgtca atgacgcccg gcgtgttttg gtaatgctcc ttgattttgg
    12001 ccaccaccgc tttcttccgg tcgtcgtcgc aatccatgcg gatttcaggc gtggcaaacg
    12061 ttttcggaac gccggcgagg agctcgctca gcttcagacc tgtctgagag agaatctcca
    12121 gcagtcttac agcggcgtaa atggcgtcgt catatccgaa atagcggtcg gcaaaaaaca
    12181 ggtggccgct catttctcct cccagcaggg ctttttcctc cttcatcttg gccttgatca
    12241 gcgaatgccc ggccttccac atcagcggcc ggccggaata ttccgcaatg ccgtcgtaga
    12301 gaacctgcga gcacttgact tcgccgatga tggtcgcccc gggatggtcc ttgaggatga
    12361 agcgcgcaaa caaaagaagc agcttgtctc cccagatgat ctcgccggtg tcgctgatca
    12421 ctccgatgcg gtccgcgtct ccgtcaaagg cgatgcccag gtcagccttt tctttggccg
    12481 taagccgaat gagttccgca agattttctt cgacggtggg gtccgggaag tgatggggaa
    12541 agcggccgtc cggctcacag tagagggcag tgacgtcgca gcccattctc tccagaatgg
    12601 gaagggcaaa tttccccccg acgccgtttc ctccgtcgag gacaatcttc agcttttttg
    12661 atacgcggac ggagtcgaag atatagtcct gataaatctt cgtgatgtcc tgcgcctgcg
    12721 cggacccttt gccggaatga taacttccgg attccatgat gcgcctcagc tcctgaatct
    12781 gctccccgtg gatggaatcg tagccgatgc agattttaaa gccgttgaac tccggcgggt
    12841 tgtggctgcc ggtgatcatg acgccgcctc ccgctcctaa gcggcggatg gaaaaataca
    12901 gcatgggggt cgcgcacagg ccgatgtcga tggtgt
//
LOCUS       DAOSIM010000035        12787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_4958_length_12787_cov_7.317939, whole genome shotgun sequence.
ACCESSION   DAOSIM010000035 DAOSIM010000000
VERSION     DAOSIM010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 12787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12787
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4958_length_12787_cov_7.317939"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="PLR47_02855"
     CDS             <1..1066
                     /locus_tag="PLR47_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOG11907.1"
                     /translation="GTSRNWRDWRWQLRHRATSLSALARFLERPSGGGEKLRSALSRY
                     PMAITPYYLSLIQPDNDQDPLALQCVPDAREAAFSAGLADDPLREDRSMPVPNLIQRY
                     PDRVLAIVTHRCATYCRHCSRKRFWKTSEDSSLKSRLTAMVRHVSKTPQIREVILSGG
                     DPLMFDDASLELILSAFASIPHVEVLRIGSRAPVVLPMRITGNLCRILKRHRPLWFNT
                     QFNHPSEITPDAARACNRIQEAGIPVSNQSVLLKGVNDSPEVMKSLLNGLQKISVRPY
                     YLFHCEPARGCNHFRTDVRTGLKLMDKLRQSCSGLALPQYVADLPGPEGKAPLWPLSR
                     PIRNALRKHQDFFDNFEELD"
     assembly_gap    1170..1265
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1266..1671
                     /locus_tag="PLR47_02860"
     CDS             <1266..1671
                     /locus_tag="PLR47_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOG11908.1"
                     /translation="FRSVDVFEPADLREKKMQYLYKEGDKFCFMDNETFEQVYLTEGQ
                     VGDAVNFLLDNIEAEILLFEDRPIGISLPNFVELVVTEAEPWAKGDTVSGATKPVKVQ
                     TGYTILVPTFISEGEKIRIDTRTGEYLTRVKG"
     gene            1677..2615
                     /gene="epmA"
                     /locus_tag="PLR47_02865"
     CDS             1677..2615
                     /gene="epmA"
                     /locus_tag="PLR47_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HOG11909.1"
                     /translation="MSTLRQEDSFRLARKSQALRLRAEFTQCLRRRFLEQGFLEVETP
                     LRIPSPAPEEHIEALASDGWFLQTSPELCMKRILAAGYPRIFQFCKCFRAGERGGRHL
                     PEFSMLEWYALHTDYQMLMRQCEALLQSALADTGHSEKIIWRNQIIRLPLPWERITVK
                     DAFSRYAPVSLQNALAGGRFDEVLTEHVEPNLGCDHPVFLCDYPASMAALAKLKEGDP
                     AVAERFELYMGGMEIANGFSELTDAQEQRRRFEEALEARAAKDLARYAMPEPFLAALE
                     GLPPCAGIALGMDRIIMILADAPNIDDVVAFPPETL"
     gene            2722..3417
                     /gene="tolQ"
                     /locus_tag="PLR47_02870"
     CDS             2722..3417
                     /gene="tolQ"
                     /locus_tag="PLR47_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HOG11910.1"
                     /translation="MESHFHGSLLGMILDAGLMVQFVLLILLIFSIISWTIIFIKFRT
                     YRRIRQENEAFDSDYQRSTKLSDLLPASKKYPFSTTAKVFRVGYTEMTKANRLSRDAA
                     RTEDISLSSLDNVERALNRASSTEMTKLESALGFLATTGSASPFIGLFGTVWGIMETF
                     KSIGARGSATLAVVAPGISEALIATAAGLAAAIPAVIFYNYFLNKSRTMVQEMDNFAD
                     EFLNIVERYLVRK"
     gene            3479..3865
                     /gene="tolR"
                     /locus_tag="PLR47_02875"
     CDS             3479..3865
                     /gene="tolR"
                     /locus_tag="PLR47_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HOG11911.1"
                     /translation="MAEINVTPLVDVMLVLLIIFMVAAPMLSMGIDVNLPRVKSKSID
                     LAEEKLILSINENREIYLNKTRLPVQDLNAKLAAIFAARIDREVFLRADKNVPYGFVV
                     EVMSEIRKAGVDKLGMITEPPPEGAK"
     gene            3919..4590
                     /locus_tag="PLR47_02880"
     CDS             3919..4590
                     /locus_tag="PLR47_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOG11912.1"
                     /translation="MFLASLFLHLVLMAAVFATVPGSSKPLTFGAPYSVSLVGPEVIG
                     PGSQEAPDQKSLLKTISSDSSVILKREIGASPSSFVMKKDDSGRSDIEKAIRALEQKE
                     TAAGKNKNPAAASTPSGTAASQEYASQLNAYSRFVWAKVKRNWSLPATLMPKKNVEAV
                     IEVRIAQSGSLEHIGFEKRSGNSYFDESALRAVQKSTPFPPPGGWASGRFVEIGIRFH
                     SAELR"
     gene            4617..5933
                     /gene="tolB"
                     /locus_tag="PLR47_02885"
     CDS             4617..5933
                     /gene="tolB"
                     /locus_tag="PLR47_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HOG11913.1"
                     /translation="MDKKYRLALALILVSLWMVSAAGFAAGKVYIDIDAPGFQQFPVA
                     ILDFQNQKAGTGASPNDPADSIRRYLDLTGIFRVLDKKSFLEGIEPGSPEVIRFSDWS
                     AIGADFLLRGNLRDGGNGLSAESRLYDVTRGQVIFEKKYSAPDGSPQTLGLSIASDIL
                     LALTGDAGDFRTRIAFVIKKKSGSDIFTMNYDASDLKNMTNDHSIVAAPRWSPDGRYF
                     AFTSYKNGRPMVFLRHLKTGKEKTIASFSGLNLCGSFSPDGRKILLTLSKDGNEEIYA
                     LDIETMHLNRLTNSFSIDVSPAWSPDGRKIAFVSNRSGSPQIFVMDADGSNVKRVTFE
                     GNYNTSPSWSPRGGRIAYEGLINRKYQIFSADEEGNNLIQLTFDEADNEYPSWSPSGR
                     QIVFHSRQNGKSRICIMNANGMNLRVLKESRDLLVMPAWSPRPGKQ"
     gene            6025..6657
                     /gene="pal"
                     /locus_tag="PLR47_02890"
     CDS             6025..6657
                     /gene="pal"
                     /locus_tag="PLR47_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HOG11914.1"
                     /translation="MNRHLTVIGLLLIFVFSLTIFSGCAEKQAVVKDEAMQEQKVAAA
                     QEGAPAPVAADDDAALRAKEAADREAALKEQAAKDEAARKAAARAAWAKKNAEALVDL
                     NIQNIYFDYDKSGIRPDAREILKANAEIFTKNSAAKIIVEGHCDERGTAEYNMALGER
                     RAQEAKQYLVNLGIDASRIETISYGKERPLDNRSIDEAWAQNRRAQFLLK"
     gene            6682..7506
                     /gene="ybgF"
                     /locus_tag="PLR47_02895"
     CDS             6682..7506
                     /gene="ybgF"
                     /locus_tag="PLR47_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HOG11915.1"
                     /translation="MKRFWYLILPAMVFSLAACATSSDLKALRTELNQQMEAKFAAAD
                     TRLDDRVSAVQNAQAKNTEDLAALRKATANTGVNISDLRDQVQQLRGQVETLKKETAR
                     DPKKDAEVQEKIDQLLVKVNFIENFLEIGKKDAAGAGNGATPGKDHGKQGKAAVYAAA
                     YSTFKNGQYTKAREAFQDFLTAHPTGEYSDNAQFWIGECYFFEKNYEKAILEYEKVTK
                     KFPSSNKVPYALLKQGLCFQNLGDKVSARLLLQQVIKDYPNTNQARIARSKLQEIK"
     gene            complement(7575..8357)
                     /locus_tag="PLR47_02900"
     CDS             complement(7575..8357)
                     /locus_tag="PLR47_02900"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HOG11916.1"
                     /translation="MRHGYAPAAGIMAMLLLSLLACGCAAGLAFTGPASSPDSAERIR
                     RTPGETDRIVATARMDLSATEGHYPLRAALVLQKPSFLRLEALPVIGTPGFFLSATPK
                     EMRIWIPSQNEFYAGSPSAENMARFLPWALNLEDLVMILSGACPRLDAAGVTYRHSEE
                     SGGLRVDMKTPSGPSQTIWMEKSGPLTRLVRYAPDGKEIYSVRYEGYGPSLALPGKIF
                     IQWADREASVRVEYDDLKIEPSADPSVFALPVPDGVKIIRLD"
     gene            complement(8397..10100)
                     /locus_tag="PLR47_02905"
     CDS             complement(8397..10100)
                     /locus_tag="PLR47_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG11917.1"
                     /translation="MQKKHIPLLICGLLFWALLAGCASFPARTRPAAPDPAPETYSKE
                     AAYHYSLAVLSRLDGRPAESIDHMKRALSFSPDSPYLNTELVSLYVENGDVEPALALG
                     EATLARQPGNIELRSIMGGLYFNLREYDKAVQEYRTVTETDPDNAVAYLYLATIYSQE
                     KKYAEAEKAYRELLALDPDNIIGMFYYAKTLSQMNRPADAESLYQKIIAQRPAFEAAW
                     TGLAALYESRNRYDEAIGVYRRYLAVHPARIGIRVKIAELQMKANREEAAEKELREVL
                     KISPDNREVIMALGLLYFDMRRYDEAAAEFALLLEKTPADDKLRYLLASVLEQKGDRQ
                     KALTEYQKISPKFELYANAQIQAARLTNLEGDSAGAIGIIRRAIGQKKDQAVLHLYLS
                     SLYEEKKDMAAAEKTIREGISLFPGNTDLHYLLGALLEKTGRLEESIQSMRRVLSLDP
                     GNADALNFIGYSYADRGIHLDEAEQMIVQALKIKPDSGYILDSMGWVHFRKSNYESAL
                     TYLKKALEFLPEDPGVMEHLGDVYFETGRRQEALEWYRKAIKADPANSRLKNKMDLLK
                     P"
     gene            complement(10148..11098)
                     /locus_tag="PLR47_02910"
     CDS             complement(10148..11098)
                     /locus_tag="PLR47_02910"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOG11918.1"
                     /translation="MKKKKKIKSAAAVLIVLIVLAVAAIFFMVLSDREETEKVAPPRA
                     AEKRAKPSAAESRAAAKTQPRAAKDAVPPPESGVGTEAAPSRTEGRQVAIIIDDIGQD
                     MKPVRELLDIDANITFAVLPLLPHSREAAETLHRAGREILLHLPMEPLSYPKEKPGSG
                     ALFTDMNNEEILFQMERNMASVPYASGVNNHMGSKFMADEEKLTLVFREIKKRNLFFI
                     DSRTTGNSKTQAAAQRARLRVASRKIFLDNERDYSRIYRILMEAARASDGNSPLIVIG
                     HPYPETIRAVREAGKVFREQGIAIVPVSALLSKSKPEGAS"
     gene            complement(11133..12452)
                     /locus_tag="PLR47_02915"
     CDS             complement(11133..12452)
                     /locus_tag="PLR47_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOG11919.1"
                     /translation="MKNISGKWPVLAVILFGLFVFFGPYSDSRVSARDKNIYKEIKTF
                     NEVLDMVQKNYVEDIDSSVLIQGAINGMIKSLDPHSAFMTPELYRELEVETQGRFGGI
                     GIEITILKDVLTVVSPIEDTPAFKAGVKSGDSIIKIDGKSTKDITIMEAVKKLRGPKD
                     TKVTITIMRENMVQPKDITLTRAVIEVKSVRAKALDDHIGYIRIASFQERTAQDLRKA
                     LTDVSEKTRPMKGLVLDLRNDPGGLLTQAIEVSDMFLKSGVIVSTRGRTKKMETKNTA
                     RDSGDKEVVVPMVVLVNEGTASAAEIVAGAVQDNGRALIVGTQTFGKASVQTVIPLED
                     GSALKLTTARYYTPNGRSIQAEGIQPDIVVKLVRPAEEPENPPGERLREKDLKGHIKP
                     AKEDTAKPDDNQNGDTRETTDLTRDNQLKTAIDILKSWDIMKKNLAK"
     gene            complement(12580..>12787)
                     /gene="cysS"
                     /locus_tag="PLR47_02920"
     CDS             complement(12580..>12787)
                     /gene="cysS"
                     /locus_tag="PLR47_02920"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes a two-step reaction; charges a cysteine
                     by linking its carboxyl group to the alpha-phosphate of
                     ATP then transfers the aminoacyl-adenylate to its tRNA;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOG11920.1"
                     /translation="PDLFFSKDRDIEAEKRGIDIPRVEALIAERQKARDDKDWGRADE
                     IRRELASQSIALKDAAGRTDWLID"
BASE COUNT         2757 a         3747 c         3457 g         2730 t
ORIGIN      
        1 tggaacttcc cgcaactggc gggattggcg ctggcagttg cgccatcgcg caacctccct
       61 gtcggcgttg gccaggtttc tggaaagacc gtccggcggc ggagaaaagc tgcgcagcgc
      121 gctgtcgcgc tatcccatgg ccattacgcc ttactacctg tccctgatcc agccggataa
      181 tgatcaagac cctctggccc tccagtgcgt tccggatgcc agggaagcgg ctttctccgc
      241 aggccttgcg gatgaccccc tgcgcgaaga ccgctccatg cctgttccga acctgattca
      301 acgctatccc gaccgcgttc tggccatcgt cacgcaccgc tgcgccacct actgccggca
      361 ttgcagccgc aagcgcttct ggaaaacttc cgaagactct tctttaaaaa gccgcttgac
      421 cgccatggtc cgccacgtat cgaaaacacc gcagattcgt gaggtcatcc tctccggcgg
      481 cgacccgctg atgtttgacg atgcctctct ggaattgatc ctgtcagcgt ttgcgtccat
      541 accgcacgtc gaggtgctgc gcatcggcag ccgcgccccc gtggtcctgc cgatgagaat
      601 cacgggaaat ctctgccgga tcctgaaacg gcaccggccg ttatggttca atacccagtt
      661 caaccacccc tcggaaatca ccccggacgc cgcgcgggcc tgcaaccgga ttcaggaagc
      721 gggaattccg gtttccaatc agtcggttct gctcaaaggc gtcaacgact cgccggaggt
      781 catgaaaagc ctgctgaacg gtttgcagaa gatatcggtt cgtccttatt atctttttca
      841 ctgcgaaccc gcgcggggct gcaatcattt ccgcacggac gtccggacgg ggctgaagct
      901 catggacaag cttcgtcaaa gctgttcggg tctggcgctg ccgcaatacg tggccgatct
      961 gcccgggccg gagggaaaag cccccctctg gccgctgtcg cggccgatcc gcaatgcttt
     1021 gcgaaaacat caagattttt ttgacaattt tgaagaattg gattaggaag agccccaatg
     1081 ccgttcattc atggccgtca acgaaggaga aagaattttt atgtacaccg ccagtgattt
     1141 gagaaaaggc ctgcgcctga aaatcgacan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnncttcc gctccgtcga tgttttcgag cccgccgacc tgcgcgaaaa gaaaatgcag
     1321 tacctgtaca aggaggggga caaattctgc ttcatggaca acgagacctt cgagcaggtc
     1381 tatctgaccg aggggcaggt cggagacgcc gtaaactttc tgctcgacaa tattgaagct
     1441 gaaatcctgc tctttgagga ccgccccatc ggcatcagcc tgcccaactt cgtagagctg
     1501 gtcgtcaccg aggccgaacc gtgggccaag ggcgacacgg tgtccggcgc gaccaaaccc
     1561 gtcaaggtgc agaccggcta taccattctg gtcccgacct ttatcagcga aggcgaaaaa
     1621 atacgcatcg acacgcgcac gggagaatat ctgacgcggg tcaaaggcta gccgttttga
     1681 gtacgctccg gcaggaagat tcttttcgcc tggcgcgcaa gtcgcaggcc ctgcggctca
     1741 gagccgaatt cacccaatgc cttcgccggc gcttcctgga gcagggcttt ttggaagtgg
     1801 aaacccccct gcgcatcccc tcgcccgctc ccgaagaaca tatcgaagcc cttgcgtcgg
     1861 acggctggtt tttgcagacg tctcccgaac tctgcatgaa aaggattctg gccgccggct
     1921 atccccgaat attccagttt tgcaaatgct ttcgcgcggg cgaaagaggc ggcaggcacc
     1981 tgccggaatt ttcgatgctg gagtggtatg ccctgcatac cgactatcaa atgctcatgc
     2041 gccagtgcga agcgcttctc caatccgccc tcgcggatac gggtcattcc gaaaagatca
     2101 tctggcggaa tcaaatcatc cgtctgccgc ttccctggga gagaatcacc gtgaaggacg
     2161 ctttttcccg atacgcgccc gtcagcctgc aaaacgctct ggccggcggc cggtttgacg
     2221 aagtcctgac cgaacacgtc gaacccaacc tcggctgtga ccatccggtg tttctttgcg
     2281 attatccggc ctccatggcg gcgcttgcta aactgaaaga gggtgatccg gccgtcgcgg
     2341 agcgctttga gctctacatg ggcggcatgg aaatcgccaa cggcttttcg gagctcaccg
     2401 acgcacagga acagagacgg cgctttgagg aagccctgga ggctcgggcg gcaaaggatc
     2461 tggcccgtta tgccatgccg gagcctttcc tggcggcgct cgaaggcctg cccccctgcg
     2521 ccggcatcgc gctgggcatg gaccggataa tcatgattct ggccgacgcg ccgaacatcg
     2581 acgacgtcgt cgcctttccg ccggaaaccc tgtagccgaa aaaaaagatg tattgacaag
     2641 cctttttttt tcctttaggt tacggagcaa acacagacag gatcgcgaca cgagaaagta
     2701 aataattttg gagagttatg catggaaagc cattttcacg gttctctgct gggaatgatt
     2761 ctggatgcgg gattgatggt tcagtttgtg ctgttgatcc tgctgatttt ttccattatt
     2821 tcctggacga tcattttcat caaattccgc acctaccgca ggatccggca ggaaaacgag
     2881 gcctttgact ccgactacca aagaagcacg aaattgtccg atctcctccc tgcatccaaa
     2941 aaatacccct tctccacgac ggcgaaagtg ttccgcgtcg gctacacgga aatgaccaag
     3001 gcaaaccggc tctcccggga tgcggcgcgg accgaagata tcagcctgtc ctccctggac
     3061 aacgtggaac gggcgctcaa ccgcgcctcc agcacggaaa tgaccaaact ggagtccgcg
     3121 ctgggatttc tggccacaac cggttcggcc agccccttca tcggtctgtt cggaacggtc
     3181 tggggaatca tggaaacctt caaatccatc ggcgccagag gttcggcgac gctggccgtc
     3241 gtggcccccg gcatctcgga agccctgatc gccacggccg ctggccttgc cgcggccatt
     3301 cccgccgtta ttttttacaa ttactttttg aacaaatcca gaacgatggt gcaggaaatg
     3361 gataatttcg cggacgaatt tctgaacatc gtcgaacgct atctggtccg caagtaagcg
     3421 aagttcgggt gagacaacga tgcgaaaggc gcgcagaaga gaatctgaaa aaagatccat
     3481 ggcggaaatc aacgtcacgc cgctcgtgga cgtgatgctg gtgctgctga tcatcttcat
     3541 ggtggcggcg ccgatgcttt ccatgggcat tgacgtcaat ctgccgcgcg tcaagtcaaa
     3601 aagcatcgac ctcgccgagg aaaagctgat cctcagcatc aacgaaaacc gggaaattta
     3661 cctcaacaaa accaggctgc cggtgcagga cctgaacgcc aagctggcgg ccattttcgc
     3721 cgcgcgcatt gaccgggagg tttttctgcg cgccgataaa aacgttcctt acggctttgt
     3781 ggtggaggtc atgtccgaaa tacgcaaagc gggcgtcgat aaactcggca tgatcaccga
     3841 gcctcccccc gagggcgcaa aatgatgtcc ccgctgcccc tcaaaaggta tttcggaaaa
     3901 aacggtagcc ccggcaacat gtttctggcc tcattgtttc ttcacctcgt gctcatggcc
     3961 gccgttttcg cgacggttcc cggttcatca aagccgctga cgttcggagc tccttattcc
     4021 gtttcgctgg tcgggcccga ggtcatcgga cccggcagcc aggaggcacc tgatcaaaaa
     4081 agccttttaa aaacaatatc atcggatagc tccgtcattc tgaagcgcga aatcggcgcc
     4141 tccccgtcat cgtttgtcat gaaaaaagac gacagcggca gatccgacat cgaaaaagcg
     4201 atccgcgctc ttgagcagaa ggaaacagcc gccggtaaaa acaaaaatcc ggcggcggct
     4261 tcaaccccct ccggaaccgc cgcatcgcag gaatatgcgt cccagctaaa cgcctacagc
     4321 cgttttgtct gggccaaagt aaaaagaaac tggtcgcttc ccgcaaccct gatgcccaaa
     4381 aagaatgtcg aagccgtaat cgaagtgcga atcgcccaaa gcggctcgct ggaacacatc
     4441 ggcttcgaaa agcgctccgg caacagctat tttgatgaat cggccctccg cgcggttcaa
     4501 aaatcaaccc cctttccgcc gcccggagga tgggcgtccg gccgtttcgt ggaaatcggc
     4561 atccggtttc attccgcgga gctgcgctga cgcacccgca atcaaggacc gcaaccatgg
     4621 ataaaaaata tcgcctggcc ctggcattga tcctcgtttc tttgtggatg gtgagcgcgg
     4681 ccgggtttgc cgccggcaag gtctatatcg acatcgacgc accgggtttt cagcagtttc
     4741 ccgtcgcaat tctggatttc caaaaccaaa aggcggggac gggagcctcc ccgaatgatc
     4801 ccgccgacag cattcggcgc tatctcgacc tgacgggaat ctttcgcgtc ctggataaaa
     4861 aaagcttcct ggaagggatt gagccgggat cacccgaggt catccgcttt tccgactggt
     4921 cggcgatcgg cgctgatttt ctgctgcggg gcaatctgag ggacggagga aacggcctgt
     4981 ccgcggaaag ccgtctgtat gacgtcacgc gcggccaggt gattttcgaa aagaaatatt
     5041 cggccccgga cggcagcccg cagacgctgg ggctctccat cgcttcggac attcttctgg
     5101 cgctgaccgg ggatgcagga gacttccgca cccggatcgc tttcgtgatc aaaaaaaaat
     5161 ccggctccga cattttcacc atgaattatg acgcttccga tctgaaaaac atgacgaatg
     5221 accacagcat cgtcgccgcg ccccgctggt cgccggatgg ccggtatttt gcctttacat
     5281 cctataaaaa cggccggccc atggtgtttc tgcgccattt gaagaccgga aaagaaaaaa
     5341 ccatcgcttc tttttccggc ctcaatctgt gcggttcctt ctcgccggac ggcagaaaaa
     5401 tcctgctgac gctgagcaag gacggcaacg aggaaattta tgccctggat attgaaacga
     5461 tgcatctgaa tcgcctgacg aacagttttt cgatagacgt ttcccccgcc tggtctcccg
     5521 acggccggaa aatcgccttt gtctccaacc gctccggctc cccgcagatc ttcgtcatgg
     5581 acgcggacgg aagcaacgtg aagcgggtca ctttcgaagg caactacaac acgtctccat
     5641 cctggtctcc ccgcggggga aggatcgcct atgaaggcct gatcaaccgg aagtatcaga
     5701 ttttctccgc ggatgaggaa ggcaacaacc tgattcagct gactttcgat gaagcggaca
     5761 acgaataccc ttcctggtct ccctccggca ggcagattgt tttccactcc cggcaaaacg
     5821 gcaaaagccg aatctgcatc atgaacgcca acggaatgaa cctgcgcgtc ttgaaggaaa
     5881 gcagggactt gctggtgatg ccggcatggt ccccgaggcc cggaaaacag taagattcca
     5941 taatttttat tgacgcgaag ccgcaagtta tgttttaatt cgcgcaaatt ccgacatact
     6001 ataaaataac gaaaggagaa catcatgaac agacatttaa ctgtaatcgg attacttctg
     6061 atttttgtct tcagtttaac catattcagc ggttgcgcgg aaaaacaggc ggtagtgaag
     6121 gatgaggcga tgcaggaaca gaaggtggcg gctgcgcagg aaggcgctcc ggcgcctgtt
     6181 gcggccgatg acgatgccgc cttgagggca aaagaggccg cggacaggga agccgcgctg
     6241 aaagaacagg cggccaaaga cgaagcggcc agaaaagcgg ccgcccgcgc ggcctgggcc
     6301 aaaaagaacg cggaggcgct ggtggatctc aacatacaga atatttactt tgattacgac
     6361 aaatccggca tccggcccga tgcacgcgaa atcctgaaag ccaacgccga aatcttcacc
     6421 aaaaacagcg ccgcgaaaat cattgtggaa ggccactgcg atgaaagagg aacggccgaa
     6481 tacaacatgg ccctgggcga aagacgcgct caggaagcca agcagtacct ggtcaatctg
     6541 ggcattgacg cttcccggat tgaaaccatc agctacggca aggaacgtcc gctggacaac
     6601 cgcagcattg atgaagcctg ggcgcagaac agacgggcgc agtttctttt aaaatagccg
     6661 ggtctgccac gggagacatt tttgaaacgc ttttggtatc tcatactgcc ggcaatggtt
     6721 ttttcccttg ccgcatgcgc gacctcttcg gatttaaagg cgctccgcac cgagctgaac
     6781 cagcagatgg aagcaaaatt cgccgcggcg gacacccggt tggatgaccg ggtgtccgcc
     6841 gtgcaaaacg cgcaggcgaa aaacactgaa gacctggccg cgctgcgcaa ggcaaccgcc
     6901 aacaccggcg tcaacatctc tgaccttcga gatcaggtgc agcagctgcg gggtcaggtc
     6961 gagaccctga agaaggaaac cgcccgggac cccaaaaaag acgctgaagt tcaggaaaag
     7021 atcgaccagc tgctggtgaa agtcaacttc atcgagaact ttctggaaat cgggaaaaaa
     7081 gacgcggcag gagccggcaa cggcgcgact cccggcaagg atcatggaaa gcagggcaag
     7141 gcggcggtct atgcggcggc ttactcgacc tttaaaaacg gccagtacac gaaggccagg
     7201 gaggcgtttc aggacttttt gacggcgcat ccgacaggcg agtattccga caatgcccag
     7261 ttctggattg gcgagtgcta tttttttgaa aagaactatg aaaaagccat tctggaatat
     7321 gaaaaagtaa ccaaaaaatt ccccagcagc aataaagtgc cctacgcgct tttaaagcag
     7381 ggactctgct ttcaaaatct tggcgacaag gtcagcgcca ggctcctttt gcagcaggtc
     7441 atcaaggact atcccaacac caaccaggcg agaatcgccc gttccaaact tcaggaaatc
     7501 aaatagggac aaaaccttcc gtctcccgcc gatgatccgt ggaccgggtc cgtcttgcgt
     7561 ccgccgccgc ggcttcaatc caaacggatg atcttcacgc cgtccggaac cggcagggca
     7621 aacaccgacg gatccgctga cggctcaatc ttcaaatcgt cgtattccac gcggacggat
     7681 gcttcccggt ccgcccattg aatgaaaatt tttccgggca gggcaagcga aggcccgtag
     7741 ccctcatagc gcaccgaata tatttccttg ccgtccggag cataccggac cagcctcgtc
     7801 aagggtccgc ttttttccat ccagatcgtt tgcgaaggcc ccgaaggggt tttcatgtcc
     7861 acgcgcagtc cgccgctctc ttcggaatgc cggtacgtca cgccggctgc gtccagacgc
     7921 gggcaggcgc cggaaagaat catcaccagg tcttcaagat tcagcgccca aggcaggaaa
     7981 cgggccatgt tttcagcgga ggggcttccc gcgtaaaatt cattctgcga cgggatccat
     8041 attctcatct ctttcggcgt cgcgctgaga aaaaaaccgg gcgtgccgat caccggaagc
     8101 gcctccaggc ggagaaagga aggcttctgc aaaaccagag cggcccgaag gggataatgc
     8161 ccctccgtcg cggacaggtc catccgtgcc gtggcgacga tcctgtccgt ttccccgggg
     8221 gtccgccgga tcctctccgc ggaatccgga gaagaggcgg gaccggtaaa agccaggccg
     8281 gcggcgcacc cgcacgccag aagcgacagg agcaacattg ccataatccc cgcggcggga
     8341 gcatatccat gacgcatgcg gcatatcctg agcgtgtgat acgcggacaa agggttctag
     8401 ggtttcaaaa ggtccatctt gttcttcagg cggctgttgg ccggatccgc tttgatggcc
     8461 ttccggtacc attccagcgc ctcttgccgt cggccggttt cgaaatacac gtctcccagg
     8521 tgctccatga cgccgggatc ctccggcaga aactccaggg ctttcttcag gtaggtcagc
     8581 gcgctttcgt agttgctttt cctgaaatgc acccatccca tggaatccag aatgtagccg
     8641 ctgtccggtt tgatcttcag cgcctgaacg atcatttgct cggcctcgtc caggtggatc
     8701 ccgcggtcgg catagctgta gccgataaag ttgagggcgt cggcattgcc cggatcgagc
     8761 gaaagcaccc gcctcatgga ttgaatgctc tcctcgagac ggcctgtttt ctccagcagc
     8821 gcccccagaa gataatgcag gtcggtgttg ccgggaaaaa gacttatgcc ttcccggatt
     8881 gttttttccg ccgcggccat gtctttcttc tcttcataca gagacgacag atacaggtgc
     8941 agcacggcct gatctttttt ttggccgatg gcccggcgga taatcccgat cgcgcccgcg
     9001 gaatctcctt ccagattggt cagcctggcc gcctggatct gggcgttggc gtacagctca
     9061 aacttcgggg agatcttctg atactcggtc agggcctttt gccgatcccc tttttgctcc
     9121 agaacgctgg ccagcaggta ccgcagcttg tcgtcggcgg gcgttttctc caaaagcagc
     9181 gcaaactcgg cagccgcctc gtcataccgg cgcatatcga agtacaaaag ccccagcgcc
     9241 atgatcactt cccggttgtc ggggctgatc ttcagcactt cccgcagttc cttttccgcc
     9301 gcttcctccc ggtttgcttt catctgcagc tccgcaatct ttacgcggat gccgatccgg
     9361 gcgggatgga cggccagata gcgccggtaa acaccgatgg cttcatcata ccggttccgg
     9421 ctttcataca gcgcggcaag tcccgtccag gccgcctcaa aggccggcct ttgcgcgata
     9481 attttctgat agaggctctc cgcgtccgcc ggccggttca tctggctcag ggttttggcg
     9541 taataaaaca tcccgatgat gttgtcggga tcgagcgcca gcaattcccg gtaggccttt
     9601 tcggcctcgg catatttctt ttcctggctg taaatggtcg ccagataaag atacgcgacg
     9661 gcattgtccg gatccgtttc cgtgacggtc cggtactcct gaacggcttt gtcgtattca
     9721 cgcagattga aatagaggcc gcccatgatg gagcgaagtt cgatgttgcc cggctgcctg
     9781 gccagggttg cttcgcccag cgccaaagcc ggctcgacat cgccgttttc aacgtaaagc
     9841 gagacgagct cggtattcaa ataaggcgaa tcgggggaaa aggaaagcgc gcgcttcatg
     9901 tgatcgatgg attctgccgg acggccgtcc agcctggaca ggacagccag ggaatagtga
     9961 taggcggcct ctttggaata tgtctccgga gcgggatcgg gcgccgccgg acgcgtccgg
    10021 gcgggaaaag aagcgcaacc ggccaaaagc gcccaaaaca gcaatccgca gatcaggaga
    10081 ggaatatgct ttttttgcat aggagacgtt tgccttcgct tcctgttttc ggagcagcag
    10141 ggaattttca ggatgcccct tccggctttg acttcgaaag cagcgccgaa accggaacga
    10201 tggcgatccc ctgttcccgg aagactttgc cggcctcccg aacggcccga atggtttcag
    10261 gatacggatg gccgatcacg atcagcggcg agtttccgtc cgacgcccgg gcagcctcca
    10321 tgagaatccg gtaaatcctg gaatagtccc gttcattgtc cagaaagatt tttctggacg
    10381 caactctcag acgggccctt tgcgccgccg cctgcgtctt tgaattcccc gtggtccggg
    10441 aatcaataaa aaacagattt ctctttttaa tctcacgaaa aaccagcgtc agcttctcct
    10501 cgtccgccat gaatttggaa cccatgtgat tgttgacgcc ggaggcatac ggcacggacg
    10561 ccatatttcg ctccatctga aagaggatct cctcgttgtt catatccgta aaaagagcgc
    10621 cgctgcccgg cttttccttc ggataggaaa gcggctccat gggcagatgc agaagaattt
    10681 cgcggccggc ccgatgaagc gtttccgccg cttcgcgcga atggggaaga agcggcagaa
    10741 cggcaaaggt gatgttcgcg tcaatgtcca gcagttcgcg caccggcttc atgtcctggc
    10801 cgatgtcgtc gatgataatc gccacctgcc gcccttccgt ccgggacggt gcggcttctg
    10861 tcccgacgcc gctttccggc ggcggcacgg cgtcttttgc cgcacggggc tgtgttttcg
    10921 cggcggcccg gctttccgcg gcggaaggct ttgcccgctt ttccgccgca cgcggcggtg
    10981 cgactttttc cgtttcttca cggtccgaca ggaccatgaa gaagatggcg gcgacggcca
    11041 ggacgatcag aacgatcaga acggctgccg cactcttgat ctttttcttc tttttcatgg
    11101 ccgtcggtgt tgaacccccg gaatccgcat cctcatttgg ccaggttttt cttcatgata
    11161 tcccagcttt tcaaaatatc gatcgccgtc ttgagttgat tgtccctggt cagatcggtc
    11221 gtttccctgg tgtctccgtt ttgattgtcg tcgggcttcg ccgtatcttc tttggccggt
    11281 ttgatgtgtc ccttgagatc cttttcccga agcctttccc ccggcggatt ttccggctct
    11341 tccgccggcc gcactagctt gacgacaatg tccggctgaa tgccctcggc ctgaatggac
    11401 ctgccgttgg gcgtatagta ccgggccgtc gtcaatttca gcgcggaacc gtcctccagg
    11461 ggaatcacgg tctgcacgga cgccttgccg aaggtttgcg tccccacgat cagcgcccgg
    11521 ccgttgtcct gcacggcgcc cgccacgatc tccgccgcgc tggccgtgcc ttcgttcacc
    11581 aggaccacca tgggcacgac gacttccttg tcgccgctgt cgcgcgccgt gtttttggtt
    11641 tccatctttt tcgtccggcc gcgggtggac acaatcacgc ccgacttcag aaacatgtcg
    11701 gacacctcga tggcctgggt cagaagcccg cccggatcat ttcgcaaatc cagcaccagt
    11761 cctttcatgg gcctggtttt ttcggacacg tcggtaagcg ctttgcgcag gtcttgagcg
    11821 gtccgctcct ggaaagaggc gatccggatg tagccgatat ggtcatccag ggccttcgcc
    11881 ctgacgcttt tcacctcgat cacggcgcgg gtcagcgtga tgtccttcgg ctgaaccatg
    11941 ttttcccgca tgatcgtgat ggtcaccttg gtgtctttcg ggccgcgcag ttttttcacg
    12001 gcctccatga ttgtaatgtc ttttgtggat ttgccgtcaa tcttgatgat cgaatcgccg
    12061 gatttcacgc ccgccttgaa cgccggcgtg tcctcaatgg gggacaccac cgtcagcacg
    12121 tccttcagga tggtaatttc aatgccgata ccgccgaagc gcccctgcgt ctccacttcc
    12181 agttccctgt atagttcagg cgtcatgaat gcgctgtgcg gatcgaggga cttgatcatg
    12241 ccgttgatcg cgccctggat cagcaccgaa gaatcgatat cctccacata gttcttctgc
    12301 accatgtcca gcacttcatt gaaggtttta atctccttgt aaatgttctt gtcccgcgcg
    12361 gaaaccctgc tgtcggaata aggtccgaag aagacaaaaa gcccgaagag gatcacggcc
    12421 aacaccggcc acttaccgga aatatttttc atgcttcgcc tcactgaccc gcaaagattt
    12481 tatcgcacgc aaacgcaatg attttcatga ccggcattat ataccactcc tttttttctt
    12541 ttccatctat tttcgtgaaa cagcgaaccg gcccggttct caatcgatga gccagtcggt
    12601 ccggcccgcc gcatccttca aggcaatgct ctgcgaagcc agctcccggc ggatttcatc
    12661 agcgcggccc cagtctttat cgtcgcgggc tttttgccgc tcggcaatca gcgcctctac
    12721 ccggggaata tcaattccgc gtttttcggc ttcgatgtcg cggtccttcg agaaaaagag
    12781 atccggg
//
LOCUS       DAOSIM010000036        12766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_4968_length_12766_cov_8.341830, whole genome shotgun sequence.
ACCESSION   DAOSIM010000036 DAOSIM010000000
VERSION     DAOSIM010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 12766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12766
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4968_length_12766_cov_8.341830"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLR47_02925"
     CDS             <1..282
                     /locus_tag="PLR47_02925"
                     /inference="COORDINATES: protein motif:HMM:NF028226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HOG11921.1"
                     /translation="HKPDIHGYHQLKTRKAGNFRFIEFHIKVDPQMTVEASHGIARDV
                     KHAILTQYPAATVTVHVEPCDGNCTEICVAGCLLPEDRRRGIARCARSL"
     gene            complement(507..1463)
                     /locus_tag="PLR47_02930"
     CDS             complement(507..1463)
                     /locus_tag="PLR47_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG11922.1"
                     /translation="MKTKDLYLQDIRPGDKIASSFLVTEKNMAFSQKGSAYLNVRLKD
                     KTGEIDAKVWDNAVALDRVFKKGDIISIEGRAQSYRNAVQISILTAKTCGREDVDPSD
                     YLPASKLDTAAMFEDLRAYVGTMSSEPLKKLLDAFLNDEKTADLFRRAPAAKGFHHIY
                     LGGLLEHTLSVVRLLDRAAEHYPQLNRDLLIAGGILHDIGKIYEFSYDGIIEYSDEGR
                     MIGHLVMGVEMINQKMAAIDDFPARLALELRHIILSHHGEFEFGSPKRPKTREALVIH
                     FMDDLDAKLNGFESFIEGDAANPGSEWTAYNRFFERYLYKGK"
     gene            complement(1491..3404)
                     /gene="selB"
                     /locus_tag="PLR47_02935"
     CDS             complement(1491..3404)
                     /gene="selB"
                     /locus_tag="PLR47_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOG11923.1"
                     /translation="MQHFVLGTAGHVDHGKTSLIKALTGVDTDRLKEEKERGITIELG
                     FASLSLPSGHTLGIVDVPGHEKFIRNMVAGAAGIDLVLMVIAADEGVMPQTKEHLQIC
                     SLLGITTGMVALTKIDLVEQDWLELVRSEISEYLQGTFLAGAPIVPVSASQGQGIPAL
                     LEEIDKTVRRLGEKADDGIFRLPVDRIFTMKGFGTVVTGTLISDKIKIGEEVQILPEK
                     ISARVRGIQVHNQSTEEAFSGQRTALNLQGIERSTIERGNVLVRPETVWPTQRLDVFF
                     EYLASNARKLKNRSLARLHTGTAEIMVRFVLLDADELSPGDAAFAQLILENEDVVVGG
                     DHFVLRSYSPVTTIGGGRILDPHPGKHKRKNQKILDDLRLLQSGALPEKILVMLDRAG
                     FHGIPIRSLAFRLGASVKKIREALEKLFSDRRAVLLSNEDATALSAGLYAHLEELLIK
                     SLANYHRSHPLKEGISKEELKAALPEAVSGKLFNMVLAGLARKERIASDKDNVRLASH
                     HVQLEGEEDALRQAIASVYAQAGLAPPSLSDVMVRFRDQKARAQNIVRLMIKDGDLVK
                     INEELCFARAAMDKLREDYKGQLIRDGQATPATFKDLTGLSRKYIIPLMEYFDASKLT
                     VRVEDHRILREKP"
     gene            3568..3993
                     /gene="ruvX"
                     /locus_tag="PLR47_02940"
     CDS             3568..3993
                     /gene="ruvX"
                     /locus_tag="PLR47_02940"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOG11924.1"
                     /translation="MRILGVDYGEKRIGLAVSDGLGLTAQGLPTLIRKTKKNDLEILR
                     RRILDYSVERIVIGYPVRIDGSEGIQCEKVNRFAGRLAETFMLPVVKWPETLTTKEAE
                     DILRASGVHWRKRKDKVDRLAACLILQDYLDNAGKREPG"
     gene            4033..5076
                     /gene="mltG"
                     /locus_tag="PLR47_02945"
     CDS             4033..5076
                     /gene="mltG"
                     /locus_tag="PLR47_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOG11925.1"
                     /translation="MKSRTKKTLFYFFSLPAIAAVVFGALLFIYAVTPMDSAKDKSGV
                     VDIPTGTSLIRVINILHNAGLVQNRVLFHALVVVKGGARSVRAGEYEFAADISPSVLV
                     DKLLHGEIKQYRVTIFEDSSISQVASRLREYKLINEKDFFELAQDREFLSFLDIQSAS
                     IEGHLFPDTYFLDRSMTTRQIMRIMVERYHSRVSPEMIRKAAQKGLNANQFVTFASLV
                     GKESGYAAEKPMIAAVFYNRLKKGMPLQCDPTAVYDLKNFKGKVKRFHYKRESPYNTY
                     QIRGLPPGPIASPGLDSFQAVLNPAGVDYLYFVSKGDGTHFFSSSLVAHNDATIRFRN
                     MMNREEKTDEVVR"
     gene            5357..5803
                     /gene="mraZ"
                     /locus_tag="PLR47_02950"
     CDS             5357..5803
                     /gene="mraZ"
                     /locus_tag="PLR47_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOG11926.1"
                     /translation="MSVFRGQYHHTIDEKGRIIFPSRFRELFAEKYDNRMVITNWDGY
                     LIAFPYAEWRLIEEEVSKRRLLDKKFRDFQRFFMSGAVDCNLDSQGRVLVPPTLREYA
                     HLEKDIVLAGMLKNIEIWPRDKFEERNREAGEKIDRDNEIAEQLGI"
     gene            5806..6741
                     /gene="rsmH"
                     /locus_tag="PLR47_02955"
     CDS             5806..6741
                     /gene="rsmH"
                     /locus_tag="PLR47_02955"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOG11927.1"
                     /translation="MSLVFSHEPVMREEVLSWLVRNQSGVYVDGTIGGGGHALAILEN
                     TRASLVGIDLDAEAISAARQRLDSFQPRVKLVKGNFADLASVLDGFNIEKVDGVILDL
                     GVSSHQLDAAHRGFSFSQSAPLDMRMDRDAKLRAYDIVNGFKPSELERVIRLYGEEKM
                     AARIAGAIVRRRDVSPIETTAELAQLITQAMPHSMKHQKIHPATRTFQAIRIAVNREL
                     DTLAPGIEAAVAALSTGGRLGVISFHSLEDRIVKHTFRDLAATCVCPKDVPRCVCHKQ
                     AVVKVLTRKAVTPSLEEIRRNPRARSAKLRVAERI"
     gene            6743..7090
                     /gene="ftsL"
                     /locus_tag="PLR47_02960"
     CDS             6743..7090
                     /gene="ftsL"
                     /locus_tag="PLR47_02960"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOG11928.1"
                     /translation="MAQTAGTETLTQGRPLKEADAASFGVKYPAFIVVALVIMFVAVI
                     YVGSHIRMTQMEYDVAAALHTKEQLMEEQKKLKLELAMLKSPQRIEDIARTQLQMTYP
                     TAAQVIVLKQTRK"
     gene            7093..9081
                     /locus_tag="PLR47_02965"
     CDS             7093..9081
                     /locus_tag="PLR47_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOG11929.1"
                     /translation="MAADSKKWLKFRLASILAIFLILFIALLSRAFQLQVLSGEKLKR
                     LADRQHMTTLSIHAERGMIYDRNGEKLAMSVMAESVCADPSRISNPAKVSREISSILN
                     LDPSSVLKKISEPKSFCWLARKISSQQALMVESANLEGIFLIKEPKRFYPNGPLAAHL
                     IGFSGFDAEGLEGLERKYDRYLKGTPEKLTWARDAKGKKLFVHVEQDPSEKMGSANLV
                     LTIDSRIQYLAETHLKEAVLEKGAKGGVVIVMDPKTGEILAMANEKAFNPNNIKGLTS
                     ENWRNRAITDSFDPGSTFKPFLVAAALEEKVVKESDRFYCENGHYQVANRVIREANHK
                     KHGYLAVRDIIKYSSNIGSAKIAEKLGREKFYAYIKNFGFGSRTGIDLSGEAAGVLRP
                     VKSWTRVDTTNIGFGQGISVTAIQLVTALATIANGGVRMKPYIVKGFTDRNNQPVKMF
                     APETVRRVISPETARRMAVMLTEVVGAEDGTGKRAHIVNVPVAGKTGTAQKFDFARGV
                     YSSEKVRTSFIGFFPSDNPQVVILVMLDEPQRDKWGGVAAAPVFKSIGEQILNCFKTN
                     IRETPAFEPQPVHELQLVSADASLVEDMTAAENEINDESRMPDFKGLTIREALKMARS
                     RSIELRVSGSGWATQQFPQPGSEPGEDRLCKVIFEMKN"
     gene            9085..12051
                     /locus_tag="PLR47_02970"
     CDS             9085..12051
                     /locus_tag="PLR47_02970"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOG11930.1"
                     /translation="MMELRQLLQGIQTLQTETDPKGDVSSICYVAEACGPGSLFVAIP
                     GLAFDGHHFIGRAIERGAHFIVHQKDIVVPDGVKAIRVPDSRRALGTLAKNYFGDPSS
                     RLTLIGITGTSGKTTVSYLLESILTAAGFSCGVLGTINYRYGGKQFPAPNTTPESFDM
                     QRILSEMACAGVTHVIAEVSSHALDLKRVDDCDFDLAVFTNLSPEHLDYHKDMEDYFR
                     AKKRLFAELLPRSRKNLARKAVINADDPRGRQLIREISVPALSYSLEQDGAARVQNPH
                     VTLGGVQADILLDGEKISVTSGLIGGFNLSNILAAAAAASVLAVEPRAIARGIQNLSL
                     VPGRLEKVVPSGGVHVFVDYAHKPDALEQVLRNLHGLKEKRILTVFGCGGNRDRAKRP
                     VMGDIATTWSDLTIITSDNPRREDPLEIIREIEAGIDGGKIRKVPQDRLEFDAGAHGY
                     AILADRRAAIEAAVRIAEPQDIVLIAGKGHEDYQILGTKKVPFDDRLAAMRALRLKFP
                     GWMSPAFSLAEVLQATGGRLAAGERDQAVYGVSTDSRQIHPGNLFVALAGENFDGHAF
                     VPKAVEGGALCVLVADADRIDLKALTRRAAVIETADTLRALGDLAHMHRRRFSIPVIA
                     LTGSSGKTTTREMLTCILRQEKTVLGTEGNLNNLIGLPQTLFRMTDRHDAAVLEMGTN
                     TRGEIKRLTQIAAPDIGLVTNVGPAHLAGFGSVETVREEKGDLWLHMNPAGVAVVNLD
                     DQAVRQAAERWSGRRVTFSMSAGADVGVNDIRKNGARGVSFQLLMNGQAHKVDMKAAG
                     IHNIYNAMAASAAALACGVSVESIRRGLSMFQPVAGRMEILRLQNGAYLINDAYNANP
                     ASVREALLTLRDLASSHNAFVFLGDMLELGDAAQEMHRKIGLLLATIGTAAVFLQGDF
                     ARATAAGALEGGLAEERIVFLCDDGEAVAFLKKKLRKGDWVLIKGSRRMKMDRLAARL
                     GEEIGVEKAEDHAAAT"
     gene            12067..>12766
                     /gene="mraY"
                     /locus_tag="PLR47_02975"
     CDS             12067..>12766
                     /gene="mraY"
                     /locus_tag="PLR47_02975"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOG11931.1"
                     /translation="MIYEFLYPFHTVFSSFNVFRYITFRTIFASITALLICLVFGKWF
                     IDKLQRMQIDQQIREDGPKTHLVKKGTPTMGGMLIIFAVTVSTILWTNLSVVYVWLVL
                     LVTVGFGLIGFLDDYRKLAGKNSRGLSGKTRLTAEMIIALFVGVVLYFKPGFNAQVTI
                     PFFKTVLPNLGWGYILLCVFIIVGAANAVNLTDGLDGLATGPAGICFATYLLFAYFAG
                     NIKVAHYLQIPYVPG"
BASE COUNT         2789 a         3395 c         3773 g         2809 t
ORIGIN      
        1 cacaaacctg atatccacgg ttatcatcag ttaaagacgc gcaaagccgg aaactttcgc
       61 tttattgaat ttcacatcaa ggtcgatcct caaatgacgg tggaggcttc ccacggcatc
      121 gcccgtgatg tgaagcatgc cattttgacg caatatcccg ccgcgacggt tacggtccat
      181 gtcgagccat gcgacgggaa ttgcacggaa atctgcgttg ccggctgcct gctccctgaa
      241 gacagaaggc gcgggatcgc acgatgcgca aggagcctct gaccgggatc tggggcggga
      301 tattcgttaa atcattgaca atccaattcc cgtatcctat ggataaataa ataaaaaaat
      361 tgcagagagg agtagcgcgc tgaaaaagta gaaaaacagc ggacggaaaa taaatcaggg
      421 cttttcggac ggcgtttgcc gcggagggct tttcccgcgc gaggggaagc cctctttgcg
      481 tttcttcagg cgggcagaga tgccccctac tttcccttat acagatagcg ctcaaagaaa
      541 cggttgtagg ctgtccattc ggaacccggg ttggcggcgt cgccttcgat gaaactctcg
      601 aacccgttca gtttggcgtc gaggtcatcc ataaagtgaa tgaccagcgc ttcccgggtt
      661 ttcgggcgtt tgggcgagcc gaattcaaat tcgccgtgat ggctcaggat gatgtggcgc
      721 agttccagcg cgagcctcgc gggaaagtca tcgattgccg ccattttttg attgatcatc
      781 tccacgccca tgaccaggtg accgatcatc cggccttcgt cgctgtattc aatgattcca
      841 tcataggaga attcataaat tttgccgatg tcgtgcagga tgccgcccgc gatcagcaaa
      901 tcccggttta attgaggata atgttcggcg gcgcggtcga gcaggcggac cacggaaagc
      961 gtgtgctcca gcaggccgcc caggtaaatg tgatgaaatc ctttggcggc gggcgcccgg
     1021 cggaacaggt cggccgtttt ttcgtcgttc aaaaaggcgt ccagcagttt tttcagcggc
     1081 tcggaggaca tcgtgccgac gtaagcgcgc aggtcttcga acattgccgc ggtatccagc
     1141 ttgctggccg gcaggtagtc cgatggatcg acgtcctccc ggccgcaggt tttggccgtg
     1201 aggatggaaa tctggacggc attgcggtaa ctttgcgccc ggccttcgat ggaaatgatg
     1261 tctccttttt tgaaaacgcg gtcgagcgcc acggcgttgt cccacacctt ggcgtcgatt
     1321 tcgcccgttt tatccttgag ccggacattc aggtaggccg accctttctg ggaaaaggcc
     1381 atattttttt ccgtgaccag aaacgaagaa gcaattttat cgccgggccg gatgtcctga
     1441 agatagaggt ctttggtttt caaatgcacc gcctttctga aaaatgggtc tcagggtttt
     1501 tcccgcagga ttcggtggtc ttccactcgt accgtcagtt tgctggcgtc aaaatactcc
     1561 atgagcggga taatgtattt gcgggacagt ccggttaaat ccttgaacgt tgccggcgtg
     1621 gcctgaccgt cccggatcag ctgccccttg tagtcttcac gcaatttgtc catggccgcg
     1681 cgcgcgaagc agagctcttc attgattttc accagatcgc cgtctttgat catcagccgg
     1741 acgatgtttt gcgccctggc cttctgatcc ctgaatcgaa ccatcacgtc ggaaagggaa
     1801 ggcggcgcca gtcccgcctg cgcgtaaacg gatgcgatgg cctggcgcag agcgtcttcc
     1861 tccccctcca gctgcacgtg atgggacgcc agccgcacat tgtccttgtc gctggcgatc
     1921 cgttccttcc tggcaagtcc ggcaagcacc atgttgaaaa gctttccgga taccgcctcc
     1981 ggaagcgccg ccttgagctc ctccttggaa atgccttcct tcaagggatg gctccggtga
     2041 taattcgcga gacttttgat caggagctct tccaggtgcg cgtacaggcc cgcggaaagc
     2101 gccgtggcgt cttcgttgga gagcaggacg gcacggcggt cggaaaataa tttttccagc
     2161 gcttcccgga tttttttgac ggacgcgccc aggcgaaacg cgagagagcg gatggggatg
     2221 ccgtgaaagc ccgcgcgatc cagcatcacg agaattttct ccggcaatgc gccgctttgc
     2281 agcagcctca gatcgtccag aattttctga tttttccgtt tgtgcttgcc gggatgcggg
     2341 tcgaggatcc gtccgccgcc gatggtggtg acgggggagt agctgcgcag cacgaaatgg
     2401 tctcccccta ccaccacgtc ctcgttctcc agaatcagtt gggcgaaggc ggcgtcgccg
     2461 ggggaaagtt cgtcggcatc cagcagaacg aaccgcacca tgatttccgc cgtgccggtg
     2521 tgcagacgag ccagggaacg gtttttcagc tttcgggcat tggaggccag gtattcaaaa
     2581 aacacatcca gccgctgggt gggccagacg gtctcgggcc gcaccagaac attgccgcgt
     2641 tcgatcgtcg atctttcaat gccctggaga ttcagcgcgg tgcgctggcc ggagaaggct
     2701 tcttccgtgg attggttgtg cacctgtatg ccgcggacgc gcgcggatat cttttccggc
     2761 agaatctgca cttcttcacc gattttgatt ttatcggaaa tcagggtgcc ggtgacgacc
     2821 gtgccgaagc ccttcatcgt aaagatccgg tcaaccggca gacggaatat gccgtcgtcg
     2881 gctttttccc cgagccggcg gacggttttg tcgatttctt caagcagcgc cgggatgccc
     2941 tgcccctgag acgctgaaac cggcacaatg ggggcgccgg cgagaaacgt gccctgaaga
     3001 tattcgctga tttccgatcg taccagttcc agccagtctt gttccaccag gtcgattttg
     3061 gtgagcgcga ccatccccgt cgtaatgcca agaagagagc agatttgcag gtgctccttc
     3121 gtttgcggca tgacgccttc atccgcggcg atgaccatca aaaccaggtc gatgcccgcc
     3181 gcgccggcca ccatgttccg gatgaacttt tcatggcccg gcacgtcgac gatgcccagc
     3241 gtgtggccgg agggaagcga aagagaggca aatcccagtt cgatggtgat gccccgttcc
     3301 ttttcttcct tcaggcggtc ggtgtcaacg cccgtcaggg ccttgatgag cgatgtcttt
     3361 ccgtggtcta cgtggccggc tgttcccaaa acgaaatgct gcatgttttt tcccgctggt
     3421 tcgcgcttca agatacgttc aaataacaaa ctcccgtcat tttaacaacc tgatatttgc
     3481 cggagcggct gagctacgga aacgggcctt gatttacttc ttgaaaatgc agggtggaat
     3541 tgttagaaaa catccacgag gtgatctttg cgcattttgg gagtggatta cggtgaaaag
     3601 cgaatcggtc tggcggtgag cgacgggctg ggcctgaccg cccagggact gcccacgctg
     3661 attcgcaaaa ccaaaaagaa tgatctggag attttgcgcc ggcggatcct cgattactcg
     3721 gttgagcgca tagtcatcgg ctatccggtg cgcatcgacg gatcggaagg cattcagtgt
     3781 gaaaaagtca accggtttgc cgggcggctt gccgaaacct tcatgctgcc ggtggtgaaa
     3841 tggcccgaga ccctgaccac aaaagaggcg gaagacattc tgagggcctc cggcgtccat
     3901 tggcggaaaa ggaaggataa agtcgatcgg ctggcggcgt gtctcattct gcaggattat
     3961 ctggacaatg ccggaaaacg cgagccgggc tgatccccgc cggactcgcc ttgttcggca
     4021 aatggaatga ccatgaaatc cagaacaaaa aaaacactgt tttatttttt ttccctgccc
     4081 gcgatcgcgg ccgtcgtgtt tggcgcgctg ctcttcatct atgccgtgac gccgatggat
     4141 tcggcgaaag acaaaagcgg tgtggtggat attccgacag gcacaagcct gattcgggtc
     4201 atcaatattc tccacaatgc aggcctggtg caaaaccggg ttttatttca tgcgctggtc
     4261 gtggtgaaag gaggggcccg gtcggttcgc gccggagagt atgaatttgc cgcagacatt
     4321 tccccttccg tgctcgtgga caagcttctt cacggagaaa tcaagcaata ccgcgtcacc
     4381 atttttgaag acagttcgat ttcgcaggtc gcctcgcgtc tcagggaata caaattgatc
     4441 aacgaaaagg atttttttga gctggcgcaa gacagggaat ttttatcttt cctggacatt
     4501 cagagtgctt cgattgaagg gcatctcttt cccgacacat attttctgga ccggtccatg
     4561 accacccggc agatcatgcg cattatggtg gagcgttacc acagcagggt ctcgccggaa
     4621 atgatccgca aggccgcgca aaaaggcttg aatgcaaatc agttcgtgac ttttgcgtcg
     4681 ctggtgggca aggaatcggg gtatgccgct gaaaaaccaa tgattgcggc ggttttctac
     4741 aatcggttga aaaagggaat gccgcttcag tgtgatccca cggcggttta cgacctcaag
     4801 aatttcaagg gaaaagtgaa gagattccac tataagcggg aatctcctta taatacttac
     4861 cagatccgcg gtctgccacc ggggcccatt gccagtcccg ggctggattc ttttcaggcg
     4921 gttctcaatc ccgcgggtgt ggattatctc tattttgttt ccaaagggga cgggacccac
     4981 tttttttcgt catcgcttgt ggcccacaat gacgcaacaa tccgttttcg caatatgatg
     5041 aaccgcgaag agaaaacgga tgaagtcgtc cggtaatcag cgcctttctg cttctaacta
     5101 tttaacataa ttctttttta ttattataat ccggtttgtg atttggagtg tgccgggagg
     5161 atccttttct cttcccggcg gccttgtccg tgctgttttg cagaccgatt ggctccaccg
     5221 gggcgcgttt cccattttct tagtgatatt caattattcg attatcaaaa aagattcatt
     5281 tttttcttga caaagcgatt ttccctctct atagtcggac acgtggggta aagtggttta
     5341 ttgtggaggg ggcgtgatgt ccgtatttcg tgggcaatat caccatacta tcgatgaaaa
     5401 gggaagaata attttcccgt ccaggtttcg cgaactgttt gcggaaaagt acgacaaccg
     5461 catggtaatc accaactggg acgggtattt aattgcattt ccctatgcgg aatggcggct
     5521 tatcgaagaa gaagtctcga aaagacgcct tctggataaa aaatttcgcg attttcaaag
     5581 attcttcatg tccggcgccg tggactgcaa tctggacagt caggggcggg ttttggtgcc
     5641 gccgacgctg cgggaatacg ctcatttgga aaaagacatt gtgctggcgg ggatgcttaa
     5701 aaatattgaa atctggccca gagataaatt tgaagaaaga aatcgagagg ctggcgagaa
     5761 aattgacaga gataatgaga tcgcagagca actggggatc tgaccatgag cttggttttt
     5821 tctcacgaac cggtgatgcg cgaagaagtg ctgtcctggc tggtgagaaa tcagagcggc
     5881 gtttatgtgg acggcacgat cggcggggga ggacacgccc tcgcgatcct ggaaaacacc
     5941 cgggcgagtc tggtcgggat cgatctcgat gcggaagcga tctcggccgc ccggcaaagg
     6001 ctggattcat ttcagccgcg cgtcaaactc gtcaagggga attttgccga tctggcctct
     6061 gtgctggacg ggttcaacat tgaaaaagtg gacggcgtga ttttggatct gggggtttct
     6121 tctcatcagc tggatgcggc gcacagaggg ttcagtttca gccagtcggc gccgctggat
     6181 atgcgaatgg accgggatgc aaagcttcgc gcctatgata ttgtaaacgg tttcaaaccg
     6241 tccgagctgg aaagagtgat ccggttgtac ggggaagaaa aaatggccgc aagaatcgcc
     6301 ggagcgatcg tgagaagaag ggatgtttcg ccgattgaaa ccacggcgga gctggcgcag
     6361 ctcatcacgc aggcaatgcc gcatagcatg aagcatcaga aaattcaccc ggccacaaga
     6421 acctttcagg cgattcggat cgccgtcaac cgcgagctgg acaccctggc tccgggcatt
     6481 gaagctgccg tcgcggctct ttcgaccggc ggccgtctgg gggtgatttc gtttcactcc
     6541 ctggaggacc ggatcgtgaa gcatactttt cgtgaccttg cggcgacctg tgtttgcccc
     6601 aaagatgttc cccgctgtgt ctgtcacaaa caggccgtcg tgaaagtatt aacccgcaaa
     6661 gcggtgacgc cttctttgga ggaaatccgg agaaacccgc gggcccgcag cgcaaaattg
     6721 cgcgtcgcag aaaggatttg aaatggcgca gacggccgga accgagaccc tgacgcaggg
     6781 gcggcctctc aaagaagccg acgccgcttc cttcggcgta aaatatccgg ccttcatcgt
     6841 cgtggcgctg gtcatcatgt ttgtcgcggt gatctatgtg ggatcgcaca tccggatgac
     6901 ccaaatggaa tatgacgttg ccgccgccct gcataccaaa gagcaattga tggaagagca
     6961 gaaaaaattg aagctggagc tggcgatgct caaatcgccg cagagaatcg aagacattgc
     7021 ccgaacgcag ctgcagatga cctatcccac ggcggcgcag gtgattgttt taaagcaaac
     7081 aaggaagtga ccatggcggc agactcaaaa aaatggctga aattccggct ggccagcatt
     7141 ttggcgattt ttttgattct gttcatcgcg ttgctgtcgc gcgcttttca gcttcaggtc
     7201 ctgtccggcg agaaattgaa aagactggcc gaccgccagc acatgaccac gctttccatt
     7261 catgccgaaa gaggcatgat ttatgaccgc aacggcgaaa agctggccat gtcggtgatg
     7321 gcggagtccg tttgcgcgga tccgagccgg atcagcaatc cggcaaaagt gtcccgggaa
     7381 atttcttcca tcctgaacct ggatccctca tccgtgctga agaagatttc ggagccgaag
     7441 agcttctgct ggctggccag aaaaatttct tcccagcagg cgctgatggt ggaatccgcg
     7501 aacctggaag gaatttttct gatcaaggaa ccgaagcgtt tttatccgaa tgggccgttg
     7561 gcggcgcatt tgatcggatt ttccggtttt gacgccgagg gcctggaagg gctggagagg
     7621 aaatatgacc ggtatttgaa aggaacgccc gaaaaactga cctgggccag ggacgccaaa
     7681 gggaaaaagc tctttgtgca cgtcgaacag gatccctccg agaaaatggg aagcgccaat
     7741 cttgtgctga ccattgacag ccggatccag tacctggcgg aaacgcattt gaaggaagca
     7801 gtgctggaga agggagcgaa aggcggcgtc gtgatcgtca tggatccgaa aacgggagaa
     7861 atactggcga tggccaatga aaaagcattc aatcccaaca acatcaaggg gctgacgtcg
     7921 gagaactggc ggaaccgcgc cattaccgat tcctttgatc ccggctccac cttcaagccc
     7981 tttctggtcg ccgccgccct ggaagaaaaa gtcgtcaaag agtcggaccg cttttactgc
     8041 gagaacgggc attatcaggt cgccaaccgg gtgattcgcg aagccaacca caagaagcac
     8101 ggttatctgg ccgtgcggga tattatcaaa tactccagca atatcggctc cgccaaaatt
     8161 gcggaaaagc tcggccggga aaagttttat gcctacataa aaaacttcgg tttcggctcc
     8221 cggaccggaa tcgatctctc gggcgaggcg gcgggtgttt tgcgtcccgt caaaagctgg
     8281 acccgggtgg acacgacaaa catcggtttc ggtcagggca tttcggtcac cgccattcag
     8341 ctcgtcaccg ctctggccac gatcgccaac gggggcgttc gaatgaaacc ctatatcgtg
     8401 aaggggttca ccgatagaaa caatcagccc gtcaagatgt tcgctccgga gaccgtgcgc
     8461 cgggtgattt ctcccgaaac ggccaggcgc atggccgtca tgctgaccga ggtggtcggg
     8521 gccgaggacg gcacggggaa gagagcccat atcgtcaatg ttcccgtggc cggaaaaacc
     8581 ggaaccgcgc agaaatttga ttttgcgcga ggtgtttact cctcggaaaa ggtgcggacg
     8641 tcctttatcg gatttttccc gtcggataat ccgcaggtgg tgattctggt catgctggat
     8701 gagccgcagc gcgacaagtg gggaggcgtc gccgccgcgc cggtttttaa aagcattggc
     8761 gagcagattc tgaactgttt caagacgaac attcgggaaa cccccgcgtt tgaaccccag
     8821 ccggtccatg aactgcagct ggtgtcggcg gatgcgtccc tggtggaaga catgacggca
     8881 gcggagaatg agatcaatga tgaatcccgc atgccggatt tcaaaggcct gaccattcgc
     8941 gaggcgctga aaatggccag gtcccggtcg attgagctgc gggtatccgg aagcggctgg
     9001 gcgacgcagc agtttcccca gcccggttcg gaaccgggcg aggatcgcct gtgcaaagtg
     9061 atttttgaga tgaaaaactg agggatgatg gagcttcgtc aattactgca gggaatccag
     9121 acacttcaga cggaaacgga tccgaaaggc gatgtttcat cgatttgcta tgtcgcagag
     9181 gcgtgcggcc cggggtcgct ctttgttgcg attccgggcc ttgcctttga cggccaccat
     9241 tttatcggcc gggccataga gcggggcgcg catttcatcg tccatcagaa agacatcgtg
     9301 gttcccgacg gggtcaaggc cattcgggtg cccgacagtc gccgcgcact gggtacgctc
     9361 gccaaaaatt atttcgggga cccgtcctcc cgtctgacgc tgatcggcat cacgggaacc
     9421 agcgggaaga ccaccgtgtc ctacctgctg gaatccattc tcacggccgc cggcttttcc
     9481 tgcggcgttc tgggaaccat caattatcgc tacggcggca aacaattccc cgccccgaac
     9541 acgacgccgg aatccttcga catgcagaga attttaagcg agatggcctg cgccggggtc
     9601 acgcacgtga ttgccgaggt ttcgtcgcac gcactggatc tgaagcgggt tgacgactgc
     9661 gactttgatc tggccgtttt taccaacctc agcccggagc acctggatta ccacaaagac
     9721 atggaagact atttccgggc gaaaaaaaga ttgtttgccg aattgcttcc ccgaagccgg
     9781 aagaaccttg cgcgaaaagc cgtgatcaac gcggatgatc cccgggggcg gcagctgatc
     9841 cgcgaaattt ccgttccggc tctttcctac agccttgagc aggacggcgc ggcgagagta
     9901 cagaatccac acgtcacgct gggcggcgtt caggcggaca tcctgctgga cggagaaaaa
     9961 atttccgtga cgtccggtct gataggcgga ttcaacctgt ccaacattct ggccgccgcc
    10021 gccgccgcgt cggtcctggc ggtggagccc cgggcgattg cacggggcat tcagaacctg
    10081 tcgctggttc ccggccgcct ggaaaaggtc gtcccttcgg gcggcgttca cgtttttgtc
    10141 gactacgccc acaagcctga cgccctggag caggtgctcc ggaatctgca cgggttgaag
    10201 gaaaaaagaa tcctgacggt tttcggctgc ggcggcaacc gcgaccgggc caaaaggccg
    10261 gtgatgggag acatcgcgac gacctggagc gacctgacga tcatcacgtc cgacaatccc
    10321 cggcgggaag atccgctgga gatcatccgt gaaattgaag cgggaatcga tgggggcaaa
    10381 atcaggaaag tgcctcagga ccgccttgaa tttgacgccg gcgcgcatgg atacgcgatc
    10441 cttgccgacc ggagagccgc gatcgaagcc gccgtccgga tcgccgaacc gcaggatatc
    10501 gttttgattg ccggcaaagg ccatgaagat tatcagatcc tgggaacgaa aaaagtgccg
    10561 tttgacgacc ggctggccgc catgcgcgct ttgcggctga agtttcccgg gtggatgtcg
    10621 ccggcgtttt cgctggcaga ggtgctgcaa gccaccggcg gacgcctggc ggcgggagag
    10681 cgcgatcaag ccgtttacgg cgtttccacc gattccagac aaatccatcc cggcaatctg
    10741 tttgttgccc ttgccgggga gaattttgac ggacacgcct tcgttccaaa agccgtggag
    10801 ggaggagccc tatgtgttct ggtggccgac gcggacagaa tcgatttgaa ggcgctgacc
    10861 cggcgggccg cggtgattga aacggcagac acgctgcggg cgctgggaga cctggcccac
    10921 atgcaccgtc gtcgtttttc cattcccgtc attgcgctga ccgggtcgtc gggaaagacc
    10981 accacccgtg aaatgctcac ctgcattttg cggcaggaga aaaccgttct cggcacggag
    11041 ggaaacctga ataacctgat cggccttccg cagacgcttt tccgaatgac ggaccggcac
    11101 gatgcggccg tccttgaaat gggcaccaac acgcgcggcg aaatcaaaag gctgacgcag
    11161 atcgccgccc cggacatcgg tcttgtcacc aacgtcggcc ccgcgcatct ggcggggttc
    11221 ggctcggtgg aaaccgtgcg ggaggaaaaa ggcgatttat ggctgcacat gaatccggcc
    11281 ggcgtcgctg tcgtgaatct ggacgaccag gcggtgcgcc aggcagccga gcgctggtcc
    11341 ggacggcgcg tgaccttcag catgagcgcc ggcgcggatg tcggcgtgaa cgacatccgc
    11401 aaaaacggcg cccggggagt gagttttcag cttttgatga acgggcaggc gcacaaggtg
    11461 gacatgaaag ccgcgggcat tcacaacatc tacaacgcca tggccgcgtc cgccgcggca
    11521 ttggcctgcg gcgtcagcgt agagtcgatc cggcggggcc tgtcgatgtt tcagccggtc
    11581 gccggacgca tggagattct ccggctgcaa aacggagcct atctgatcaa tgacgcctat
    11641 aatgccaatc ccgcatccgt gcgcgaggcc ctgctgacgc tcagggacct ggcaagttcg
    11701 cacaacgcgt tcgttttttt aggcgacatg ctggaattgg gcgacgccgc gcaggagatg
    11761 caccgcaaga tcggcctgct tttggcgacc atcggcacgg cggcggtttt tcttcagggc
    11821 gatttcgcgc gcgcgacggc cgcgggagcg ctggagggcg gtctggccga agaacggatt
    11881 gtgtttttat gcgacgacgg ggaagccgtg gctttcttga aaaagaagct gcgcaaaggc
    11941 gactgggttt tgatcaaagg atcgcggcgg atgaaaatgg acaggctggc ggcgcggctg
    12001 ggcgaagaaa tcggcgttga aaaagccgaa gaccatgcgg cggcgacata gtccgggaga
    12061 aaaaacgtga tttacgaatt tttatatcct ttccataccg tgttttcatc cttcaacgtg
    12121 ttccggtaca tcacgttcag aacgatcttc gcctccatca cggcgttgct gatctgcctg
    12181 gttttcggga aatggtttat cgacaagctg cagagaatgc agattgacca gcagatccgc
    12241 gaggacgggc ccaagacgca cctggtcaag aagggtacgc cgacgatggg aggcatgctc
    12301 atcatcttcg cggttacggt ttccacgatc ctgtggacga acctttccgt ggtgtatgtc
    12361 tggctggtgc tgctggtcac cgtcgggttc ggcctcatcg gctttctgga tgattaccgg
    12421 aaactggccg gcaaaaactc ccggggcctg tccgggaaaa cgaggctgac cgcggaaatg
    12481 atcattgccc tgttcgtcgg cgttgttttg tatttcaagc cggggttcaa cgcgcaggtg
    12541 acgatacctt tcttcaagac ggtgttgccc aatctgggct ggggctacat cctgctgtgc
    12601 gttttcatca tcgtgggtgc ggccaacgcc gtaaatctga cggacggcct cgacggtttg
    12661 gccaccgggc ccgcgggcat ctgcttcgcg acgtatctgc tgtttgccta tttcgccggc
    12721 aacatcaagg ttgcgcatta tctgcagatt ccctatgtgc ccggcg
//
LOCUS       DAOSIM010000037        12243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_5213_length_12243_cov_6.557187, whole genome shotgun sequence.
ACCESSION   DAOSIM010000037 DAOSIM010000000
VERSION     DAOSIM010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 12243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12243
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5213_length_12243_cov_6.557187"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLR47_02980"
     CDS             complement(<1..219)
                     /locus_tag="PLR47_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOG11932.1"
                     /translation="MIEKNVKTDFSATVSEPVIGEGTYIHPLAAVIGNVELGKNIMVS
                     PMASVRGDEGQPLHVGDDSNIQDGVIIHA"
     gene            complement(302..916)
                     /gene="coaE"
                     /locus_tag="PLR47_02985"
     CDS             complement(302..916)
                     /gene="coaE"
                     /locus_tag="PLR47_02985"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOG11933.1"
                     /translation="MLNVGLTGGIACGKSTVAEMFVRLGGHLIDFDKLAHEVQEPEKP
                     AWRDVVQCFGTEILLPDRTIDRGKLASIVFCSPEKLRRLNSIVHPRVFEEWWSRLEKI
                     KAQTPHAVVFSDVPLLFEGGMQHLFELTILVVVEPECQIERLMTRNSVCREEAQLRLA
                     SQMPIGEKIRLATIVIDNQCDPKETEKKVAAAWEDLVARERAKP"
     gene            complement(920..1696)
                     /locus_tag="PLR47_02990"
     CDS             complement(920..1696)
                     /locus_tag="PLR47_02990"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOG11934.1"
                     /translation="MLLVIDVGNTNTVLGLFDQDQLLHDWRIRTEIDHTIDEYGVLIY
                     NLYQSIRMKAKEIKSVRSIIISCVVPPMLNILEPLCIKYFNIKPMIVGPGIKTGMPIF
                     YDNPKEVGADRIVNAVAAFEKYRQETIIVDFGTATTFDYVSPRGEYMGGCISPGIVIS
                     SEALFERASKLPRVEFGKPRTIITKDTVSAIQAGIMFGYAGLVDGIVNRMKAEVKSNP
                     LVIATGGLARVVAQEARTIDKIEEMLTLDGLRIIYERNKK"
     gene            complement(1703..2494)
                     /locus_tag="PLR47_02995"
     CDS             complement(1703..2494)
                     /locus_tag="PLR47_02995"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOG11935.1"
                     /translation="MPLNQSSLKQSLAGRFIGHTLYYYEEIGSTNDEVFRLGMQGAPE
                     GTAVIAERQSAGKGRMQRTWHSPPGANIYTSILLRPRFSPGLAPQISLAAGVAVAKTL
                     EPYCPGRVFLKWPNDVQIGGKKVCGILTQMRTAGDAVDFVVAGIGVNVNWKRKEFPED
                     IRGIATSLSMEAGREISRLELIILLYENLAKCYRELAQKGFAPVRKQWLERTAMLGKP
                     VSVQFGKETIRGEAVDLDEDGSLVLLAEGQKMVKVAAGDATVGKE"
     gene            complement(2584..3426)
                     /gene="nadC"
                     /locus_tag="PLR47_03000"
     CDS             complement(2584..3426)
                     /gene="nadC"
                     /locus_tag="PLR47_03000"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOG11936.1"
                     /translation="MVSPQIKIIIESALAEDIGTGDITTQATVSPKKKGRAVAVAKEN
                     FVVAGLEVFEAAFQCLDKNIKVAKLIGDGRRAKKGDVIAEVTGSLSNILQAERVALNY
                     FQRMCGIATLTAAYVNAVGKAKAKIYDTRKTVPGLRVLDKMAVRVGGGTNHRMGLYDA
                     VLIKDNHIEAAGGITAAVAAQRKHLKRSLTIEVETKNLREVREALACGADIIMLDNMT
                     IGAMKKAVALIAGRALTEASGNVTLRRVADIAATGVDIISVGELTHSVRAADISLKVR
                     GEGR"
     gene            complement(3669..6326)
                     /locus_tag="PLR47_03005"
     CDS             complement(3669..6326)
                     /locus_tag="PLR47_03005"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOG11937.1"
                     /translation="MTIKELDKAFEPAEAEKRLYDYWLENKFFHAQDTSEAPPFSIVI
                     PPPNVTGMLHMGHALNNTLQDIIIRFKRMQGYNTLWMPGMDHAGIATQNVVEQQLRKE
                     GLSRHDLGREKFIARVWEWKEKYGGVIINQLKRLGCSCDWDRERFTMDEGLSQAVREV
                     FVRLYNEDMIYQGDYIVNWCPRCHTAISDLEVEFKADQSNLWNIRYPFADGSGEIVVA
                     TTRPETMLGDTAVAVHPDDPRYRDKIGKMVILPLMNKPIPIIADDYVTMDFGSGAVKI
                     TPGSDPNDFAMAQRHNLEIISVIDGEGIINEYGGSYKGQDRYEARDNVIADLEKGGYL
                     VGIEPYAHNVGKCYRCKTDIEPMVSKQWFVKIRPLAAKAIAAVTKKKTRIVPASWEAT
                     YFEWMNNIRDWCISRQLWWGHRIPVWYCGGCGKVIVSTVDPEKCPVCDGTSLKQDEDV
                     LDTWFSSALWPFTTLGWPKRTQALKTFYPTSLLITGFDILFFWVARMMMMGLYVMKDV
                     PFRDVYLHALVRDEKGDKMSKSSGNSIDPLVMIDRYGTDAFRFTLAAYTAQGRDVRMS
                     PERIEGYKFFVNKIWNAAKLTLSNLADYDENGSLNAPDALPEKWIKAKLNEAIDEVTR
                     SLDEYRFNDAAASIYNFIWHEYCDWYLEVSKPALYGKLSPERKQATQRTLKEVFAVML
                     QLLHPFMPFVTEELWQTLLGSDENSIMVSRFPVVNDAWQEKDARQEMELLMEIITSVR
                     NIRGEMRIPPSVKLKVLISAVDEAIKTSVENGAAYIVNLAALESLTVDVNLVEPKGVA
                     TGVIGSTKVFVPLAGIVDIAGEKARLEKELAKVAKDLEQSSRKLANRDFRDKAAPEII
                     VKEEGKLKSFQEKFSALEGALKKLKEVTV"
     gene            complement(6472..7674)
                     /locus_tag="PLR47_03010"
     CDS             complement(6472..7674)
                     /locus_tag="PLR47_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11938.1"
                     /translation="MFCRANMKKVLFAIVLFSAVLLIAACGTKARQPESALDTPEHHV
                     FTGMKLIDQEKYQDAEREFALALELNNQYSKAYAGMSLAKAGQQNYTAAFEQLEKAWD
                     TAKTGEEQLAVHIGYIRVNTQSKLACTKIGVDCEQDTSWLKKCESSYQDAVKLDASSA
                     AAPFFMGLAYKTALDYGKAGAMFAKVLEIRKEYVSQANAQWNLVQKIQRAMPGTVTGK
                     KIALLESISRADAAALFMEELKIDVLYKKRTPRTFDTSFKDPEKARSAQTVQPAAKDI
                     AGHPLKADIDGILAIGVRGLEINPQGNFEPAGALNRAEYALMIEDILIKVTGDNVLAT
                     KFIGSTSPFPDLRSDLPYFNAVMVVTSRGIMEAMDITTGEFAPLAPVPGADALLIIRK
                     IKEELKFD"
     gene            complement(7776..8732)
                     /gene="miaA"
                     /locus_tag="PLR47_03015"
     CDS             complement(7776..8732)
                     /gene="miaA"
                     /locus_tag="PLR47_03015"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOG11939.1"
                     /translation="MKSNECSSANNSHGIPLLVLGSPTATGKTSLALELAQSLEAEVV
                     NADSLQVYRYLDIGTAKPTRAERERVRHHLLDVVDPDEEYNAALYCEQARTMIGELAG
                     EGRRVLVVGGTGLYIRALLQGIIETPPVDEQIRNYYRDIRDRLGKHHLYGLLEKRDPQ
                     AAGRINPNDAVRVIRALEVLDQSGQSILELQQKHRFADCPYAVFKIGLRVERNELKKR
                     IRQRTEQMMEAGLLDEVRGLLDRGYSETLKPLQSLGYKQMISFIRKRCGLDEAKDSIS
                     RETWQYAKRQMTWFGADRAVTWFGPGETRKIEEKAREFLAQY"
     gene            complement(8701..10494)
                     /gene="mutL"
                     /locus_tag="PLR47_03020"
     CDS             complement(8701..10494)
                     /gene="mutL"
                     /locus_tag="PLR47_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOG11940.1"
                     /translation="MSRRIAVLSEEVANQIAAGEVVERPASIVKELVENAVDAGADDI
                     RVELEIGGCRSIRVVDNGSGIEQEDVALVFERHATSKIVRIDDIHDAGSFGFRGEAMA
                     SIASVARVELLTRRPDDLAGTKATAQAGSISEISPAGVPSGTQITVTELFANVPARRK
                     FLKTEATEQSACLDAVMRLALSHPEIRFSVRADGRNMFAAPAAASVADRVAMVLGRDF
                     TDHCLTIAQRKENVELGGMVSTPTWTKSNSKSVYLFVNSRFIRDNSMMHALLAAYRQV
                     MEPRRFPAAVLFLRLPGEEVDINVHPAKMEVRFKNSRIIYDLVSGAVSRALAGAQASR
                     DAVAYRLAPREMAADSSEFQKSRPYQASWGRQRDVFSRANLQRALDEDLFARAGETDV
                     PARVEDASCEERITFDHRTYLGQFAGAYLVFGGPGGLMLLDQHAAHERILLERLKATG
                     KGPVASQPLLMPEVVTLTPAQISLLTEALPLLAGIGLELEVFGRDAVVVKAMPAALPN
                     VDARLLVSDLVDGLGEGQAAPSLAEKKEKILASLACHAAIKAHAELSPAEVAALCRDL
                     EETPFNATCPHGRPIRVRFTLYEIERMFKRK"
     gene            complement(10672..11583)
                     /locus_tag="PLR47_03025"
     CDS             complement(10672..11583)
                     /locus_tag="PLR47_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11941.1"
                     /translation="MKLRFVSITVLTGILLIWASAAFCQNAISMSEWVEKVGQGSVNW
                     SAGFIEAKGIGAPPERYQGKINARPLALRAAKVDAYRNLLEITKGVQVDSHTTVRDFT
                     VESDVINTQVEGLVKGAQIVDQQYLSDGTVEVTLRMPLYGELSKIMVPISIQKRKEIP
                     PPAPVVETPAPAPPAPPVAPPPAAPAPPAAVSYTGLVVDARGIQARPAMSPRIYDEDG
                     KEVYGSANVDREYAVQQGMSGYARDLTAAQSNQRVTASPITVKGLKTGGAGRSDVVIS
                     NADAQLIRASSENASFMKKCRVMIVLD"
     gene            11845..>12243
                     /locus_tag="PLR47_03030"
     CDS             11845..>12243
                     /locus_tag="PLR47_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG11942.1"
                     /translation="MDYKTTLNLPQTDFPMKANLAVREPEMLKKWEHMDIYHKIRKEA
                     KGRKPYILHDGPPYANGNIHLGTALNKIIKDIVIKAKNMTGFDGVYVPGWDCHGLPIE
                     HQVDKELGGKNSGLSQVEKRRACRVYAEKYV"
BASE COUNT         2475 a         3652 c         3295 g         2821 t
ORIGIN      
        1 cgcgtgaatg atcacgccgt cctgaatatt ggaatcatct cccacgtgga gcggctggcc
       61 ttcgtcgccg cgcacggacg ccatcggcga gaccatgatg ttttttccca gttccacatt
      121 gccgatgacc gccgccaggg gatgaatata cgtgccctcc ccgatcaccg gctcggacac
      181 ggtcgcgctg aaatcggttt ttacgttttt ctcaatcatg cttgccctcc gcttctctgt
      241 aataggtccg cgccggcttg tccggaatca tgcggcgcgt gctgatgttt ctcttttttc
      301 ttcaaggctt cgccctttcc ctggccacca gatcttccca ggcggccgcc accttttttt
      361 ccgtctcctt cggatcgcat tggttgtcga tcacgatcgt cgccagccgg atcttttcgc
      421 ctatcggcat ctggctggcc agccgcagtt gagcttcttc ccggcagacg gaattgcgcg
      481 tcatcaggcg ttcaatctgg cattcgggct cgaccacgac cagaatcgtc agctcaaaca
      541 ggtgctgcat gccgccttca aacaggagag gcacgtcgga aaaaacgacg gcgtgagggg
      601 tctgcgcctt gatcttttcg aggcgggacc accattcctc aaacacgcgg ggatgaacga
      661 tggagttgag acgcctgagc ttttccggag agcaaaaaac aatggacgcc agtttgccgc
      721 ggtcaatggt ccggtcgggc aataggattt ccgttccgaa gcactgcacg acatcccgcc
      781 acgcgggttt ttccggctcc tgcacttcgt gggccagctt gtcgaaatca atcagatgac
      841 cgcccaggcg cacaaacatt tccgcaacgg ttgattttcc gcaggcaatt ccgcccgtta
      901 atccgacatt cagcatgcgt tacttcttat tgcgttcgta gatgatccgc aaaccatcca
      961 gcgtcagcat ctcctcaatc ttatcgatcg tcctggcttc ctgcgccact acccgcgcca
     1021 gcccccccgt ggcgatgacc agagggttgc ttttgacttc cgccttcatc cggttgacaa
     1081 tgccgtccac caggcccgcg tagccgaaca tgatgcccgc ctgaatggcg ctgacggtgt
     1141 ccttggtaat gatggtcctg ggtttgccga attcgacgcg gggcagcttg gaagccctct
     1201 caaaaagcgc ctcgctggag atcacgatcc ccggcgaaat gcagccgccc atgtattcgc
     1261 cccggggcga cacatagtca aacgtcgtcg ccgtcccgaa atcgacaatg atcgtttcct
     1321 ggcggtactt ttcaaaagcg gccacggcat tcacgatccg gtccgcgccg acttccttgg
     1381 gattatcata aaagatcggc atgcccgtct taatgccggg gccgacgatc atcggcttga
     1441 tattgaaata cttgatgcac aggggttcca gaatattcag catcggcggg acgacgcagg
     1501 aaatgataat cgaccgaacg gatttaatct ctttcgcctt catgcggata gactggtaca
     1561 gattgtaaat cagcacgccg tactcatcga tggtatgatc gatttccgtc cgaatccgcc
     1621 agtcatgcag cagttgatcc tggtcaaaaa gccccaggac ggtattggta ttgccgacat
     1681 caatcaccag cagcatgcat ccttactcct tcccgacagt tgcgtctccc gccgcaacct
     1741 tcaccatttt ttgtccctcc gccagaagca ccagggaacc gtcttcatcc aggtcaaccg
     1801 cttctccgcg gatcgtttcc ttcccgaatt ggaccgaaac cggcttgcct agcatcgcgg
     1861 tccgctccag ccactgcttt cgaaccggag caaaaccttt ttgagcgagt tccctataac
     1921 attttgccaa attttcatac aggagaatga tcagctccag tcgggaaatt tcccggccgg
     1981 cctccatcga cagggatgtg gcgattcccc gaatatcctc cggaaattct ttccgtttcc
     2041 agttgacgtt cacgccgatg cccgccacga cgaaatcgac ggcgtccccc gccgttctca
     2101 tctgcgtcag aatgccgcac accttctttc cgccgatctg cacatcattt ggccatttca
     2161 ggaaaacgcg gcccggacaa tagggttcaa gcgtttttgc aacggccacg cccgccgcga
     2221 gcgaaatctg cggcgcaagg ccgggtgaaa agcgcgggcg caaaaggatg gacgtgtaaa
     2281 tgttcgcgcc gggaggcgaa tgccaggtcc gctgcatcct tccctttccc gcgctctggc
     2341 gctcggcgat caccgcggtc ccttccggcg ccccctgcat gcccagccgg aacacttcgt
     2401 cgttggtcga gccgatttct tcgtaatagt acagcgtgtg gccgatgaat cgaccggcaa
     2461 ggctttgctt cagggatgac tggttcaaag gcataaagtt aagagtttta agttttaagt
     2521 taagggttgt tcgctgtcgg aacggttgtg actcaattcc tgcttcagcc ttcagcctat
     2581 cgcctatcgc ccctcgcctc ttaccttcaa cgatatatcg gccgcgcgga cggaatgggt
     2641 gagttctcca acggaaatga tgtcgacgcc cgttgcggcg atgtcggcga cgcgccgcag
     2701 ggtaacgttt ccggaagcct cggtgagcgc gcggcccgcg ataagcgcca cggccttttt
     2761 catggcgccg atcgtcatgt tgtccagcat gatgatgtcc gcgccgcagg ccagggcttc
     2821 ccggacttcc cgcaggtttt tggtttccac ctcgatggtg agtgaccgct tcaaatgctt
     2881 tctctgggcg gcaaccgccg cggtgatgcc gcccgcggct tcgatatggt tgtccttgat
     2941 cagaacggcg tcatacaggc ccatgcggtg attggttccg ccccccaccc gaacggccat
     3001 cttgtccagc acccgaagac ccggcacagt ctttcgcgtg tcataaatct tcgccttcgc
     3061 tttgccgacg gcattcacgt aagcggccgt cagcgtcgca atgccgcaca tgcgctggaa
     3121 ataattcagg gcaacgcgtt ccgcctgcag aatattggac agactacccg tgacttcggc
     3181 gatgacatcg cctttcttgg cccgtctccc gtctccgatc aattttgcaa ctttgatatt
     3241 tttgtccagg cattgaaacg ccgcctcgaa aacctccagg cccgcgacga caaaattctc
     3301 cttggccacc gcgaccgccc tgcccttctt tttcgggctg acggtggcct gcgttgtgat
     3361 gtcccccgtg ccgatatctt cggccagcgc agattcaatg attattttaa tttgaggaga
     3421 aaccatttct tataccaaaa caatctgagt gatttgtaat tgacgggagg tgcaatcgct
     3481 tttcaccctc cctcaatccc tcccctcaag ggagggaaga aaggcaaccg cagggagcgg
     3541 cttgtaacct cgtgtgccct tcagggcatc aggctatcac gaccgttccc tgcaaaaccc
     3601 gcaatcgggc agaaatccaa ccacaggaaa cggttttaaa acaccttcgc gggtggagcg
     3661 accgttccct acaccgtcac ctccttcaat ttcttcaacg ccccttccag ggcggaaaat
     3721 ttttcctgga agcttttcag tttgccttct tccttcacaa tgatctcggg cgcggccttg
     3781 tcgcggaaat cccggttggc cagcttccgg gagctttgct ccagatcttt ggccactttg
     3841 gccagctctt tttccagacg cgccttttct ccggcaatgt ccacaatgcc ggccagcgga
     3901 acaaaaacct tcgtcgaacc gatcacgccg gtggcaaccc ctttgggttc aaccagattc
     3961 acgtccaccg tcagggattc cagagcagcc agattgacga tgtaggccgc tccgttttcc
     4021 acggaggttt tgattgcctc atccacggcg gaaatcagga ctttcagctt cacggaaggc
     4081 ggaatccgca tttccccccg aatgttccgg acggaggtga tgatttccat cagcagctcc
     4141 atttcctgcc tggcatcttt ttcctgccag gcgtcgttga ccacgggaaa ccggctgacc
     4201 atgatcgagt tttcgtcgct ccctaagagc gtctgccaga gttcttcggt gacaaacggc
     4261 atgaaggggt gcaaaagttg cagcatgacg gcgaaaacct ctttcagggt cctctgggtc
     4321 gcctgcttgc gctcagggct gagtttgccg tacagagcgg gtttgctcac ctcaagatac
     4381 cagtcgcagt attcatgcca gatgaaattg tagatggaag ccgccgcgtc gttgaaacgg
     4441 tattcatcga ggcttcgggt cacctcgtcg atggcctcgt tgagttttgc cttgatccat
     4501 ttttccggca gcgcgtcggg cgcgttcagt gagccgtttt catcgtaatc cgccagattg
     4561 gaaagcgtca gtttggcggc gttccagatt ttattgacga agaatttata gccttcgatg
     4621 cgctccggcg acatgcggac atcgcgtccc tgggcggtat aggcggccag cgtgaagcgg
     4681 aaagcgtccg tgccgtaccg gtcgatcatt acaagcgggt cgatgctgtt tcccgaggac
     4741 ttgctcatct tgtcgccttt ttcatcgcgc accagcgcgt gaaggtagac gtcccgaaaa
     4801 ggcacatcct tcatcacgta aagacccatc atcatcatgc gggccaccca gaaaaagaga
     4861 atgtcgaacc cggtgatgag cagagaggtc ggataaaatg ttttgagcgc ctgcgttcgc
     4921 ttgggccagc ccagcgtcgt gaatggccac agggccgaac tgaaccaggt atcgagcaca
     4981 tcctcatcct gtttcaggga cgtaccgtca cagacggggc atttttccgg gtccaccgtg
     5041 gaaacgatca ccttgccgca gccgccgcaa taccagaccg ggatgcggtg accccaccac
     5101 agttgccgcg agatgcacca gtcgcggatg ttgttcatcc attcaaaata cgtggcctcc
     5161 caggaggcgg gcacgatgcg cgttttcttt ttcgtgacgg cggcgatggc cttggcggcc
     5221 agcggcctga ttttgacgaa ccactgtttg gacaccatcg gctcgatgtc cgttttgcag
     5281 cggtagcact tcccgacgtt gtgcgcgtaa ggttcgatgc cgaccagata gccgcccttt
     5341 tccaggtcgg cgatgacatt gtcgcgcgcc tcgtagcggt cctgtccctt gtaggatccg
     5401 ccgtattcgt tgatgatccc ctctccgtcg atgacggaga tgatctccag gttgtggcgc
     5461 tgcgccatgg cgaagtcgtt ggggtcggaa ccgggagtga tcttcaccgc tccggagccg
     5521 aaatccatcg tcacgtagtc gtcggcgata atcggaatgg gcttgttcat cagaggcaaa
     5581 atgaccattt tgccgatctt gtccctgtag cgcggatcat cggggtgcac ggccaccgcc
     5641 gtgtcgccca gcatggtttc cggccgcgtc gtggcgacga cgatttcgcc tgagccgtcg
     5701 gcaaacgggt agcggatatt ccacagattg ctctggtcgg ccttgaattc cacttccaga
     5761 tcggaaatgg cggtatggca gcgcggacac cagttgacga tgtagtcgcc ctgatagatc
     5821 atgtcttcgt tgtagaggcg gacaaaaacc tcgcggaccg cctgggaaag accctcgtcc
     5881 atggtgaagc gctcgcggtc ccagtcgcag gaacagccca ggcgcttgag ctggttgatg
     5941 atgacgccgc cgtatttttc cttccattcc cagacgcggg cgatgaattt ttcgcggccc
     6001 agatcgtgac gggagagccc ctccttgcgg agctgctgct ccacgacgtt ttgcgtcgcg
     6061 atgcccgcat ggtccatgcc cggcatccaa agcgtgttgt agccctgcat tcttttgaag
     6121 cggatgatga tgtcctgaag ggtattgttg agcgcgtggc ccatgtgcag cattccggtc
     6181 acgttgggag gcggaatgac gatggaaaaa ggcggcgctt cgctggtgtc ctgcgcgtgg
     6241 aaaaacttat tctcgagcca gtaatcgtac agtctttttt cggcctcggc cggttcaaat
     6301 gccttgtcca actctttgat tgtcatgtaa gtcttactct cctgtctgcg cgcaatcgca
     6361 aagggcctgc gccctttgcg gacggacttt ttcgccacgt ccgtcctcgc gtaaaatttg
     6421 aggcgggaaa aaacccttcc cgcctcgtgg atgtgtcaac atcattttct tttaatcgaa
     6481 tttcagctct tccttgatct tgcggatgat cagaagagcg tccgctccgg gaacgggcgc
     6541 aagcggggcg aactccccgg tcgtgatgtc catcgcctcc atgatgcccc gggaggtcac
     6601 caccatgacc gcgttgaaat acggcaggtc agaccggaga tcaggaaagg gcgaggtcga
     6661 tccgataaac ttggtggcca gcacgttgtc acccgtcact ttaattaaaa tgtcttcgat
     6721 catcagggcg tactcggcgc ggttcagggc gccggccggc tcgaaattgc cctgcggatt
     6781 gatttccagg ccgcgaacgc cgatcgccag gatcccatcg atatccgcct tcaggggatg
     6841 gccggcaatg tctttggcgg caggctgtac cgtctgggcg ctccgggctt tttcgggatc
     6901 cttgaaggac gtgtcaaatg ttctgggcgt tctcttttta tacagaacgt cgatttttaa
     6961 ctcctccatg aacagagccg cggcatcggc gcgggaaatg ctctccagaa gggcaatctt
     7021 tttgcccgtc acggttccgg gcatggcccg ctggattttc tggaccagat tccactgggc
     7081 attggcctgg ctgacatatt ctttgcggat ttcaagaact tttgcaaaca tggcgcctgc
     7141 cttgccgtag tcgagggccg tcttgtacgc cagccccatg aaaaacggag cggcggcgga
     7201 agaagcgtcc agcttcaccg cgtcctgata cgagctttcg cactttttca gccagcttgt
     7261 gtcctgctcg cagtccacgc cgattttcgt gcaggcaagc ttgctctgcg tattcacgcg
     7321 gatataaccg atgtgaacgg caagctgttc ttcgccggtc tttgccgtat cccacgcctt
     7381 ctccagttgt tcaaaggcgg cggtataatt ctgctgaccg gctttcgcca ggctcattcc
     7441 ggcataggct ttggaatatt gattgttcag ttccagcgcc agtgcaaatt cccgttctgc
     7501 gtcctgatat ttttcctgat caatcagttt catgccggta aagacatgat gctccggagt
     7561 gtcgagcgcg ctttccggct gacgggcctt cgtcccgcat gcggcaatca gcaaaacagc
     7621 cgaaaacaaa acgatcgcaa agagaacctt tttcatgttc gcgcggcaaa acatacattc
     7681 gctcctttct gcaaaagggt tattccctga aaattccttc tgtttccttc ccaaaaagct
     7741 ttgtatcatt agcttcagtg ccttgtgaag tcaagtcaat attgcgccag aaattcccgc
     7801 gctttttctt ctatttttcg tgtctcgccg ggtccgaacc acgtgacggc cctgtccgcg
     7861 ccgaaccatg tcatctgccg tttggcatat tgccaggtct cgcggctgat agagtctttt
     7921 gcctcatcca gaccgcatcg tttccgaata aaagaaatca tctgcttgta tccgagggac
     7981 tgaaggggtt tgagcgtttc tgaatagccc cggtccagaa gcccccgcac ctcatcgagc
     8041 agccccgctt ccatcatttg ttcggttctt tggcggattc tctttttcaa ttcgtttcgt
     8101 tccacgcgaa ggccgatctt gaagacggca tacggacaat cggcaaaccg gtgcttttgc
     8161 tgcagttcca gtatggattg gccgctctgg tccagcactt ccagcgcccg aatcacccgt
     8221 acggcatcgt tgggattgat gcggccggcc gcctgcgggt cccgtttttc aagaaggccg
     8281 taaagatgat gctttccaag tctgtcacga atatcccggt aataattgcg aatttgctca
     8341 tcgaccggcg gcgtttcgat aatgccctgc aaaagcgcgc ggatatagag gcccgttccc
     8401 ccgaccacca gcacccgcct gccctcaccc gccagttccc cgatcattgt tctggcctgt
     8461 tcgcaataca gagccgcatt gtattcctcg tccgggtcca ccacgtccag caggtggtgc
     8521 ctgacccttt ccctttccgc ccgtgtcggt ttggccgtgc cgatatccag ataacgatag
     8581 acctgcagcg aatccgcgtt gacaacctcg gcttccaggg actgggccag ctccagcgcg
     8641 aggctggttt tccctgttgc cgtaggcgaa cccaggacca gcaggggaat cccgtgcgaa
     8701 ttatttgcgc ttgaacattc gttcgatttc ataaagcgta aaccggacac gaatcgggcg
     8761 tccgtgcgga caggtggcgt taaagggtgt ttcttccagg tcgcggcaca aagcggcaac
     8821 ctccgcggga gaaagttccg cgtgggcctt gatcgctgca tggcaggcca gggacgccag
     8881 gattttctcc tttttctccg caagcgaagg ggctgcctgt ccttcgccca gcccgtcaac
     8941 cagatcggat accagcaggc gcgcatcaac attcggcagg gcggcgggca tggccttcac
     9001 cacgaccgcg tcgcggccga agacctccag ctccaaaccg atgccggcaa gcagcggcag
     9061 cgcttccgtg agaaggctga tctgcgcggg agtcagcgtt accacttccg gcatcaacag
     9121 gggctggctt gcaactggtc ctttcccggt tgccttcagg cgctccagaa gaattctttc
     9181 atgcgccgcg tgctgatcca gcagcatcag gccgcccggg cctccaaaca cgagataggc
     9241 gccggcaaac tgccccagat aggttcgatg gtcaaacgtg attcgttctt cgcaggaagc
     9301 atcctccacg cgggcgggaa catccgtttc cccggcacgc gcgaacaaat cctcatcaag
     9361 agccctctga aggtttgccc gcgaaaacac gtccctctgc cttccccatg aggcctgata
     9421 aggcctggat ttttgaaatt ccgaagagtc ggcagccatc tcacgcggcg ccagacgata
     9481 ggccacggca tccctggaag cctgcgctcc ggccagggct ctcgaaaccg ccccggacac
     9541 caaatcataa atgatgcggg aatttttgaa gcgaacttcc attttggccg ggtgcacgtt
     9601 gatgtccacc tcctctcccg gaagacgcag aaacaggacc gccgcgggaa agcgcctggg
     9661 ctccatcacc tggcggtagg ccgcgagcag cgcgtgcatc atggaattgt cgcggataaa
     9721 acgggaattg acaaacagat aaacgctttt ggaattggat ttcgtccatg tcggcgtgga
     9781 aaccatgccg ccaagttcga cgttttcctt tcgctgggcg atcgtcaggc agtgatccgt
     9841 aaagtccctg cccagaacca tggcgacgcg atccgcgacg ctcgccgccg ccggcgccgc
     9901 gaacatgttt cgtccgtccg cgcggacgga aaagcggatc tccgggtgtg aaagcgccag
     9961 ccgcatgacc gcatccaggc aggcgctctg ctcggtggct tctgtcttga gaaacttccg
    10021 gcgcgccggc acattggcaa agagctccgt cacggttatt tgcgttccgg agggcacgcc
    10081 cgccggagat atttcggaaa tcgagcctgc ctgcgctgtc gctttcgttc cggccagatc
    10141 gtcgggacgg cgggtcagaa gctccacgcg ggcgacggag gcgatgctgg ccatcgcctc
    10201 gccccgaaag ccgaaggagc cggcatcatg gatatcgtcg atccggacga ttttgctggt
    10261 ggcgtggcgt tcaaaaacca gcgcgacgtc ttcctgttca atgccggatc cgttgtcaac
    10321 gacccgaata gagcggcatc cgcctatttc cagctcgacg cgaatgtcat cagcccccgc
    10381 atcgacggcg ttttccacca gttccttgac gattgacgcc ggcctttcca ccacttctcc
    10441 tgcggcaatc tggttggcga cttcttcgga taaaacggca attcgacggg acaagatcca
    10501 tctcctgcaa attattttgc cgcggccaca tgatgattgg acgcggttca tcaaatccgc
    10561 ggtttgcggt ccatcgcggc ggctgagcct tcaggatggt ttccaagaaa aaagccgcag
    10621 ccaccaaaac cccgtgactg cggccctgtt gcgcaatttt acgcaagtcc tctaatccag
    10681 aacgatcatg acgcggcatt ttttcatgaa agacgcgttt tccgacgaag cgcgaatgag
    10741 ctgggcgtcg gcattgctga tcacgacatc ggaccggccc gcgccgccgg tctttaatcc
    10801 tttaaccgta atcgggctgg ccgtgacgcg ctgattgctt tgcgcggcgg tgaggtcgcg
    10861 ggcatacccg ctcatcccct gctgaacggc gtattcgcgg tccacattgg ccgaaccgta
    10921 cacttccttg ccgtcctcgt cgtaaatgcg cggcgacatg gccggacgcg cctgaatgcc
    10981 gcgtgcgtca accaccagtc cggtatagga aacagcggcc ggaggcgctg gagccgcggg
    11041 cggcggcgca acgggaggcg cgggcggtgc gggagccggc gtttcgacga cgggagccgg
    11101 aggcggaatt tctttgcgtt tctgaatgga aatgggaacc atgattttgg acaattcccc
    11161 gtacagcggc attctcagcg tcacttcgac ggtgccgtca gacaaatact gctggtcaac
    11221 gatctgcgcc cctttgacca acccttcgac ctgcgtgttg atgacgtcgc tctcaacggt
    11281 aaagtccctc accgtcgtgt gcgaatccac ttgaacgccc ttggtgattt ccaggagatt
    11341 ccggtaggcg tccactttcg cggcgcgcag cgccagggga cgggcattga tttttccctg
    11401 gtatctttcc ggcggcgcgc cgatgccttt tgcttcaata aagcccgccg accagtttac
    11461 cgaaccctgg ccgacttttt ccacccactc ggacatgctg atcgcattct gacaaaaagc
    11521 ggcggatgcc catattaaca ggatacccgt caaaacggtg atggaaacga atctcaactt
    11581 catattgtcc tccttgcatg gttggcgtcc tgaaaaattt tcctcttcga aaccggcaca
    11641 aaactaacaa aatattcttt cgcttgcaat aagaattcac agcctgtccg gcaaatgaaa
    11701 acaccgcttc cttcggcaag agttgtgctt gaaactccac ggtttatgtg atacttgagc
    11761 gtgcggataa aggctgcata caagcagatg aaatcattcg cggtttaacc gctatttttt
    11821 tgattggaca ggagcgacga caatatggat tacaaaacga cccttaatct tccccaaacc
    11881 gatttcccca tgaaggctaa cctggccgtg cgcgagccgg aaatgctgaa aaaatgggaa
    11941 catatggaca tttaccacaa gatccggaag gaagcgaaag gcagaaaacc ctatattctg
    12001 catgacggcc ctccctacgc caacggcaat attcacctgg gaacggcgct gaacaaaatc
    12061 atcaaggata tcgtgatcaa agccaaaaac atgaccggct tcgacggcgt gtacgtcccg
    12121 ggctgggatt gccacggcct gccgatcgaa caccaggtgg acaaggaact gggcggcaag
    12181 aactccggcc tgtcccaggt ggaaaaacgc cgcgcctgcc gtgtgtacgc ggaaaagtat
    12241 gtc
//
LOCUS       DAOSIM010000038        12152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_5259_length_12152_cov_7.346284, whole genome shotgun sequence.
ACCESSION   DAOSIM010000038 DAOSIM010000000
VERSION     DAOSIM010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 12152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12152
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5259_length_12152_cov_7.346284"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PLR47_03035"
     CDS             complement(<1..902)
                     /locus_tag="PLR47_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOG11943.1"
                     /translation="MFYRKILQHRLPITVAAALAGIGIMAYYATCNTGCSYLRGDILG
                     IDLKYVGVGYMGVIILLALFRQADLLRMLVAAGIGVEAFLISFQFREEVFCPFCLAFG
                     ALVILMYLINYERLHMTNARHQKWLYVFGDARIPFTGGRRLPLLSMMIAGYIFVVLTF
                     SGSATPAYAADQAAAPSYGSGPWELMIFTDYFCPPCQAAEKDLEPQLERLLAGGDVKI
                     TFVDFPGHKSSSLYAKYFLFSVAANKGHANSLKARNLLFALAVQKKVDQESVLAAVLK
                     SKNIALRTVDPKPVFQQWLAMIKR"
     gene            1127..3514
                     /locus_tag="PLR47_03040"
     CDS             1127..3514
                     /locus_tag="PLR47_03040"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOG11944.1"
                     /translation="MDQDLDNLMALLRKDHPDLSAWIVVCANDRAVDPVRLLIHDRLG
                     GILPRVEGIQSYLNRKIGEKEPLHPVPADEELLYLIQFIAEEFPGEKTPARLAANMLP
                     LITRLAEYQVGKETIAGSERFTSEQWDQLEQYLETAKAFRRWLSRKHLFLAALEASRF
                     DEILPGDREIFIGLPELTPSRERFYRKIRNERLFIDPPLFGPETAAGGPLPFDSAKNL
                     VLSFGKTVRAAQNADIEFLRLAGLHAAADWVTLEVSRFLNDRSESDRLMICLLDESLT
                     PMLWNRSLRWFGNAVNLAVWLPFSVTSAGIRLSAEIHRAGQTGRMPDFRKFAAGQAAE
                     LFANRQRYVREESEALEAAVSISTLLDDWKGRLGRHFAAAAGKFLETKKFRVTGGRSA
                     PVQVVGFGQASGENFSRGLILPVDSGIMPAQPFEGPFINPVHVPFMRKNVFECQDLLF
                     RQILAQCRKAAVAAVDDAIRERTPGYYMSLLSEEFGKPPVKVAFRRPKPDPRDEIRCA
                     VPVDEALRARLKAFTFSYSSLSKILACPFLFYHQYILGVSSPSFMDDDEKINLQMGIF
                     VHRFLQQLSTVGHDRLDDWETFFDELWESRENTELRNTEGVEIYRLNAKLILREIDED
                     ERASGRRVLFAANAISCEEKFTGTLAGRYSITGRSDRLALLSGRAEVLDFKYSKKKSG
                     FALPARETVLERFTEKGILHPAAQLILYSHFIPDVKGARFYFLKETAKDRMMELPKEA
                     LDEAGDLASAIGESLDRIVHGAALAPGDDSRQCEFCRLQSLCGKEGYYRAAGGRR"
     gene            3514..6309
                     /locus_tag="PLR47_03045"
     CDS             3514..6309
                     /locus_tag="PLR47_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOG11945.1"
                     /translation="MENVLLKASAGSGKTTQLTREVFERISAGGKFILALTFTRAATA
                     EMRSRIMDQIASTKGMPLLDRMRLILEAGRVGYSTIDSFFYRLFAALGEAPKMADEKA
                     QIDLSRDIEKRFRDGIARSGREKRLIIASRILNTDLDSLWGSLGEEQTLERCAADMER
                     LDDLEDLMKENVLLLRELESLSRAANGLADLPDGLKTRVVANLGDYDRFMSRALCGYS
                     DLEQSRGLGKNIAWHSSPYSDLNAIFKNYRKTAEKLALNKALLRELALAFLCEIYLDA
                     AKAVKEEKGLIFFADVRKALLALDPIASRERPKLMGGYFSLGLDRTEHLLVDEFQDTS
                     TRDMAILLPLIDDILSGPGERGERSFFAVGDWKQMIYGWRGANREALETAIGGYLQNG
                     VIRERSLSRNYRSTPLLVSFFNRLVENLFEGREKTETQKPPEKSGGHRDALRCVSEVS
                     LVELEKDGASEASWYPAIAEYLQKKKEEYGCAWGEMAVLALTNNHVQKIEAELASRGI
                     GVSSVKGRQLLATEEGVAVMLFLTIALSREGETAFAAAAARSPLWKDWFEDAPGIREE
                     FAAQFPRPFGLSAVSLAVERLRGKLSATVLDIWQDEAHAFFSEGGADVDEFLARMFRV
                     RWSVRVPEGENRDSIKVDTIHGTKGLQFAHVFIFWNEDEKSTPFYLPSQKCHVRFSAA
                     ETKCLQAGESTLAGEILEKRDARLMQLRRERANVFYVAATRAVRTLAVFLPVSKSGEY
                     KAVHQAVLNTFAQFAPEGSKSEMVRFSRLPEEAKGSAVWDAPSRHFGKPDPAGEIDPA
                     LISESIQAGIARGERLHRWLARVLDPGRLPPPGELGREEYGAVVRFVRRKEVFELLFR
                     PGRLYVEQPISDQKDFGVVDRMIVSDHAVTIMDYKSGSLRGLRRKYDEQRERYTRIMK
                     SLYPQRNVEYHVLPIDE"
     gene            6397..8559
                     /locus_tag="PLR47_03050"
     CDS             6397..8559
                     /locus_tag="PLR47_03050"
                     /EC_number="3.1.4.35"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP/cGMP-dependent 3',5'-cyclic-AMP/GMP
                     phosphodiesterase"
                     /protein_id="HOG11946.1"
                     /translation="MAERKPSQIFLSRGGVLIPTSGGNLQLGIPPETIKDTMRIEGGV
                     PRTFIVPKAMFDVQHGVALAEMEFPVYYNFFFRKSKTRIVCSEKQRKRIETVIAEALF
                     GPAEVDPVLEFACGEYTPGFTDLKAEMSYFRKSMIEGRCADLGDLIEFCVPDRRGKAA
                     FGETEVRFDRRGNIRVYDRGKKIASFGGDAPVMVRKTPLARVSLGFQPPIFGVTTLGA
                     GHGFDPGADTSGLILWVNRRGIMVDPPVNSTEKLAALGVSPKRIDKIILTHCHADHDA
                     GTLQKIFQEGKIHLYTTRTIYGSFMKKAAALTGIEAGLLERLVDFYPVCIGKPVIIGG
                     GAFHFHYTLHTIPTIAIQASLSGKSLVYSSDTMNDPNFIQQLYADGVLTKNRRDFLLN
                     FPWDRDLVFHEAGVPPIHTPMSYLCSLPEDVRKRMYLVHVNPDSVPDGCGLRIAPTGL
                     ANTVDLGASPMPREEAVEMLEAFSGVEWFGQMPLTKAREFLLAASVTHHRASDIIFSK
                     GDAGEAFCIVMSGEIDIVLEDRILTTYDAGGYFGEKCLFSDEARTATARAKTDVKLLS
                     IRKEEMLNLIRGTAAESLLRQIADFQNVELRDAVKDNQILDSLSSTQKTRLHGLIRPF
                     PGTLHTGDVIASKGSDARCVYMIREGRVDVYHRRSRIATLSRGDLFGVKKIFRPKEKN
                     FSFVAGEKTALYFIEQDGLRRYMEDNPGVYVKLYHSPY"
     gene            8669..9820
                     /locus_tag="PLR47_03055"
     CDS             8669..9820
                     /locus_tag="PLR47_03055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG11947.1"
                     /translation="MQSSDIQRLFHEHGRKVVLQEGDVLFCQGEESDAVYFVAGGRLG
                     VFAAQSSSEDVQINTIEAGEMVGELGAITRHPRTATLKAASPRVELLSIATPDFRALL
                     SDSLSLVEAMTLTNREHLMDADRARIQFGTSNLQMRKRVASLGQEKAQLQELLQLREE
                     LEAMVVHDLRNPLNTVIMALGLVERMKDEVKDPETFALAMRLAKGAANRMNRLIATLL
                     DIARLEAGKLALSIAQVDLNTLFEEVIEMEKAGVTKSVEIKAAAVPGLTVKADREVLW
                     RVMSNLLGNALKFAPEASTIVVEAQRGDDRKILISVTDAGPGIPEGERERIFEKFTRV
                     KNADGGAYSGTGLGLTFCRMAVEAHGGCIWVEEGPSGVGSRFCFQLPQD"
     gene            complement(9844..10440)
                     /locus_tag="PLR47_03060"
     CDS             complement(9844..10440)
                     /locus_tag="PLR47_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG11948.1"
                     /translation="MKAFAKAGLVLLAVWLALAGCSPKSYVVLMENQDGTTGKLAVSG
                     EKKETLLNQPRTAASLDGKSEKPFLVDEQKIQKDFAVALAAQPPLPVSFMLYFEAGGA
                     LLTNDSKALIPSIVEAAMKYPAPDVSVIGHTDTMGNRDYNEQISLQRARTVAQIIRDA
                     GIQVHDLTIESHGERNLLIPTPDNTPEPRNRRVEITVR"
     gene            complement(10459..10896)
                     /locus_tag="PLR47_03065"
     CDS             complement(10459..10896)
                     /locus_tag="PLR47_03065"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOG11949.1"
                     /translation="MKRFAAVFIALFWTLTPAWAADTIGYVKTASGKAEVIDAGKAVS
                     ARVGTPIQMGNLLKTGPDGSLGITFRDNTVMSFGPDTELTVDEYLYEPGKDRLKFGTS
                     MTKGTLQVISGVIAKLKPEAVQMKTPAAVIGCRGTRFLVKVEP"
     gene            complement(10925..>12152)
                     /locus_tag="PLR47_03070"
     CDS             complement(10925..>12152)
                     /locus_tag="PLR47_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOG11950.1"
                     /translation="VGASAQGLMDIRFSPMGTVMPGVEAHAQALEQILTKTYISRPSW
                     AAAIEGLTILAGGLAVGIIALSAPALVSACITAAILFAAGWGAWIAFVRYHLLLDPVT
                     PGLALLAAFILGSIIHHRTSERRHRWVREAFSRYISPNRVDYLVRHPDQLELGGRRQE
                     CSFVFTDLAGFTGLMEKMDPADAVSVLNAYLDAMVTIAFGRGGTLDRIVGDSVAIMFS
                     APVAQPDHRARALSCALEMHAFAFGYAADCRAKGIELGQTRIGVHSGEVIVGNFGGKT
                     LFDYRALGDAVNTASRLEGANKYLGTLICVSEATLQGCPEAVVRPAASLVLKGKTQPI
                     SVYEPLAAPEANGGKPERDTAYEKAFELLKRRELLALEAFERLAAERPADELVRLHRD
                     RLRSGKKGDTIILEEK"
BASE COUNT         2521 a         3418 c         3743 g         2470 t
ORIGIN      
        1 aaccgcttga tcatggccag ccactgctgg aagaccggtt tggggtccac cgtccgcagt
       61 gcgatatttt ttgatttcag gacggcggcc agaacgcttt cctggtcgac ttttttctgc
      121 acggccagcg caaagagaag gttcctggcc ttcaggctgt tggcgtggcc cttgttcgcg
      181 gcgacggaaa aaagaaagta tttcgcgtac agcgaactgc ttttgtggcc gggaaaatcc
      241 acgaaagtga ttttaacgtc tcctccggcc agcagtcttt ccagctgggg ctccaggtct
      301 ttttccgcgg cctggcaggg aggacaaaag taatccgtaa agatcatcag ctcccacggt
      361 ccactgccgt aggaaggcgc cgcggcctgg tcggccgcat aggcgggcgt tgccgatccg
      421 gaaaaggtaa ggacgacaaa aatatatccg gcgatcatca tggacagcag gggaaggcgc
      481 cggccgccgg tgaatggaat cctggcgtct ccgaaaacat agagccattt ttgatgccgc
      541 gcattggtca tatgaagcct ttcatagttg atcagataca tcaggatcac cagagccccg
      601 aacgcaaggc agaaggggca gaagacctcc tccctgaact ggaaggagat cagaaacgct
      661 tccacgccga ttcccgccgc gaccagcatt ctcagcaggt ccgcctgccg aaacagggcc
      721 agcaaaatga taacgcccat gtatcccacg ccgacgtatt tcaggtcgat gcccagaatg
      781 tctccccgaa gataggagca gcccgtattg cacgtcgcgt aatacgccat gatgccgatg
      841 ccggcaagcg cagcggcaac ggtgatgggc agacgatgct gaagaatctt tcgataaaac
      901 atgggctcga gtctttcggc ttttttaatg ggcggaatta tagagaggga tgatgaggtt
      961 gtcaataaaa tgttcacggc tgttcagggg gggaagtaac gatactgaaa tccggggatt
     1021 caattgggta ttgacaaggc ccgctcgctt cttcatagtc atggccgggc ggaaacgcct
     1081 tcgtcaaaga ctaaagaaca gaccttcaca ttcaaaggaa gaccggatgg atcaggattt
     1141 ggacaatctc atggcgcttc tgcggaagga tcaccccgac ctttcggcct ggatcgtggt
     1201 ttgcgcaaac gacagagcgg tcgatccggt gcgtctactg attcatgacc ggctgggcgg
     1261 cattcttccc cgggtggagg gaatccagtc ctacctcaac cggaagatcg gcgaaaaaga
     1321 gccattgcat ccggtgccgg ccgacgaaga gctcctgtac ctgatccagt tcatcgcgga
     1381 ggaatttccc ggtgaaaaga cgcccgcccg tctcgccgcg aacatgctgc ccctgatcac
     1441 ccggcttgcg gaatatcagg tcggaaaaga gacgatcgcg gggtccgagc ggttcacctc
     1501 cgagcagtgg gatcagcttg agcaatacct cgagacggcg aaggcgtttc gccggtggct
     1561 ttcccggaaa catttgtttc tggcggcgct cgaagcctcc cggtttgacg aaatccttcc
     1621 cggcgaccgg gagatattca tcggtctgcc cgaattgacg ccgtccaggg agcggtttta
     1681 cagaaagatc agaaacgaaa gactgtttat cgatccgccg ctgttcgggc ccgaaacggc
     1741 cgcgggaggc cctctgccgt ttgattccgc gaaaaacctt gttttgtcct ttggcaaaac
     1801 ggtccgggcg gcccaaaacg cggacatcga atttctgcgc ctggccggac ttcatgcagc
     1861 ggcggattgg gtgacgctgg aggtttcccg ctttttgaac gaccgatcgg aatcggaccg
     1921 cctgatgatc tgcctgctgg atgagtcgct gaccccgatg ctgtggaacc gttcgcttcg
     1981 ttggttcgga aatgccgtca accttgccgt ctggctgccg ttttccgtaa cgtcggccgg
     2041 aataaggctt tcagcggaga ttcaccgggc cgggcaaacc ggccggatgc cggatttcag
     2101 gaaatttgcg gccggccagg cggcggaatt gttcgcaaac cggcagcgct acgttcgcga
     2161 ggaaagtgag gcgctggaag ccgccgtatc gatcagcacg ctgttggacg actggaaggg
     2221 aagactgggg cggcattttg ccgcggcagc gggcaaattt ctcgagacga aaaaattccg
     2281 cgtcacgggc ggcaggtccg ccccggtgca ggtggtcgga ttcggccagg catcggggga
     2341 aaacttcagc aggggcctga tacttcccgt ggacagcggc atcatgccgg cgcagccctt
     2401 tgaaggcccc tttatcaatc cggtgcatgt tcccttcatg cgcaaaaacg tatttgaatg
     2461 ccaggatctt cttttccggc agattctggc gcagtgccga aaggccgccg tcgcggcggt
     2521 ggacgacgcc atccgcgaac gaacgcccgg ctattacatg agccttcttt ccgaggagtt
     2581 tggaaaaccg ccggtgaagg tcgccttccg tcggccgaaa ccggacccgc gagatgaaat
     2641 tcgttgtgcc gtcccggtgg acgaggctct gagagccagg ctcaaagcgt tcaccttttc
     2701 gtattcgagc ctctccaaaa ttctggcctg ccccttttta ttctaccatc aatacatcct
     2761 cggagtgtct tccccttcct tcatggacga cgatgaaaaa atcaatctgc agatggggat
     2821 tttcgtccac cggtttctcc agcagctctc gaccgtcggg cacgacaggc tcgacgactg
     2881 ggagacgttt tttgacgaac tgtgggaaag ccgcgaaaac acggagctca gaaatacgga
     2941 aggcgtggag atctatcggc tgaacgcgaa gctcattctg cgggaaatcg atgaagacga
     3001 acgagcgtcc ggaagaaggg tcctgtttgc cgcgaacgcg atctcgtgcg aagaaaaatt
     3061 caccggcacg ctcgccggtc gctacagcat caccggacgt tcggaccggc tggccctgct
     3121 ttccgggcgc gccgaggtgc tggatttcaa atattccaaa aagaaaagcg gctttgccct
     3181 tcccgcgagg gaaaccgttc tggaacgatt tacggaaaaa ggaatcctgc atcccgcggc
     3241 gcagttgatc ctgtacagtc attttattcc ggacgtaaaa ggcgcccgct tttattttct
     3301 caaggagacc gcaaaggacc gcatgatgga gcttccgaag gaggcgcttg acgaagcggg
     3361 cgacctggcg tccgccatcg gggagagtct cgacaggatc gtgcacggcg cggcgcttgc
     3421 gccgggggat gattcccggc agtgcgaatt ctgccggctt cagtctctgt gcgggaaaga
     3481 aggctattac cgggcggccg gagggaggcg ctgatggaaa acgttctgct gaaagcgtcg
     3541 gcaggctccg ggaaaaccac gcaattgacc cgggaggtat tcgaacggat ctccgcggga
     3601 gggaaattca ttctggcgct gacctttacc cgcgccgcca ccgccgagat gcgctcccgc
     3661 atcatggatc agatcgcgtc aacaaaggga atgccgctgc tcgaccggat gaggctgatc
     3721 ctggaggcgg gccgcgtcgg atactcgacg atcgactcgt ttttctaccg gcttttcgcc
     3781 gccctgggcg aagcgccgaa gatggccgac gaaaaagcgc agatcgatct gtcccgggat
     3841 atcgaaaagc gttttcgcga cggcatcgcg cgaagcggcc gggaaaagcg gctgatcatc
     3901 gcgtcccgta tcctgaacac ggatctggat tcgctctggg gctccctcgg ggaagagcag
     3961 acgctggagc gctgcgcggc ggatatggag aggcttgacg atctggagga cctgatgaaa
     4021 gagaacgtcc tcctgcttcg ggagctggag tctctgagcc gcgcggcaaa cggccttgcc
     4081 gatctgccgg acggactgaa aacccgagtc gtcgccaacc tgggggatta cgaccggttt
     4141 atgtccaggg cgctttgcgg ctattccgat ctggaacagt cgcgggggct cggcaaaaat
     4201 attgcgtggc attcgagccc gtactcggat ctcaacgcga ttttcaaaaa ctaccgaaag
     4261 acggccgaaa agcttgcgct aaacaaggcc ctcttgcggg aactggccct tgcgtttctt
     4321 tgcgaaattt atctcgatgc ggcaaaagcc gtcaaggaag agaaagggct gatttttttt
     4381 gccgatgtgc gaaaagcgct tctcgccctc gacccaatcg cgtcgcgcga aaggccaaag
     4441 ctcatgggcg gctacttctc gcttggcctg gaccggacgg agcacctcct ggtggatgaa
     4501 ttccaggaca cctcgactcg cgacatggcg attctcctgc cgctcatcga cgacatttta
     4561 tccggccccg gcgaacgggg cgaacggtcg ttttttgccg tgggcgattg gaagcagatg
     4621 atctacggct ggcgcggcgc gaaccgcgag gcgctggaga cggccatcgg cggctacctt
     4681 caaaacggcg tcatccggga gcggtcgctt tcccgcaact accggagcac gccgcttctg
     4741 gtttcatttt tcaaccggct ggtcgaaaat cttttcgagg gcagggagaa aacggagacg
     4801 caaaagcccc cggagaaaag cggcgggcac cgggacgcgc tccggtgcgt ttcggaagtg
     4861 agtcttgtcg agctggaaaa ggacggcgcg tcggaagctt cctggtatcc ggccatcgcc
     4921 gaatatcttc agaaaaaaaa ggaggagtac ggctgtgcat ggggggagat ggccgtcctt
     4981 gccctcacca acaaccacgt tcagaaaatc gaggcggagc tcgcctcgcg aggcattggc
     5041 gtctcctcgg tcaaaggccg ccagttgctg gcaacggagg aaggggttgc ggtcatgctt
     5101 tttctgacga tcgctctttc ccgcgaaggc gaaaccgcct tcgccgccgc agccgcccgg
     5161 tcgcccctgt ggaaagattg gttcgaagac gcgccgggaa ttcgggaaga attcgccgct
     5221 cagtttcccc ggcccttcgg cctttccgcg gtaagcctcg ccgtcgagcg tttgcgcggg
     5281 aagctgtccg cgaccgttct cgatatatgg caggatgagg cgcacgcgtt tttcagcgaa
     5341 ggcggcgcgg atgtcgatga atttctcgcc cgaatgttca gggttcgctg gagcgtgagg
     5401 gttccggagg gcgaaaaccg ggattcgatc aaggtcgata cgattcacgg gaccaagggg
     5461 cttcagtttg cgcatgtttt cattttctgg aacgaggatg agaaatccac accgttctat
     5521 cttccttcgc agaagtgtca tgtccggttt tcagcggccg agacaaagtg tcttcaggct
     5581 ggagaatcga ccctggccgg ggaaatcctg gagaagcggg acgcccgcct gatgcaactg
     5641 aggcgcgaga gagccaatgt gttttacgtc gccgccacgc gggccgttcg aaccctcgcg
     5701 gtttttctgc cggtcagcaa aagcggcgaa tacaaggcgg ttcatcaggc cgttctgaat
     5761 acgtttgcgc aatttgcgcc ggaagggagc aaatcggaga tggtccgctt ctcccgtttg
     5821 ccggaagagg caaaggggtc cgccgtgtgg gatgcgccgt cgcggcactt tggcaaaccg
     5881 gaccccgcgg gcgagatcga cccggcgctg atttccgaat ccatccaggc cggcatcgcg
     5941 cggggcgagc ggctgcaccg ctggctggcc agggttcttg atcccgggcg cctgcctccc
     6001 cccggggagc tcggcaggga ggagtatggc gcggttgtcc gttttgtccg cagaaaagag
     6061 gtttttgaac tcctgttcag gccgggccgt ctctatgttg aacagcccat atcggatcaa
     6121 aaggatttcg gcgtcgtgga cagaatgatc gtttcggatc atgccgtgac cattatggat
     6181 tacaagtcgg gctccctgcg agggctgagg cggaagtacg acgagcaaag agagcgctat
     6241 acccgcatta tgaaatctct ttatccgcag cgaaacgtgg agtatcatgt cctgcccatt
     6301 gatgaatagg aaacgcggct gggacttctc caggcgcgga agcgccgcgg aggaagtcac
     6361 ccgcatcatt ccggccgcaa agggggagaa aagatcatgg ctgaaaggaa accgtcgcag
     6421 atatttttgt cccggggcgg tgtgctgatt ccgacgtccg gcggaaatct ccagctcggc
     6481 attccgccgg agaccatcaa ggacaccatg cggatcgagg gcggcgtgcc ccgcacgttc
     6541 atcgtgccca aagcgatgtt tgacgtgcag cacggcgtag cactggccga gatggagttt
     6601 cccgtctatt acaatttctt tttccggaag agcaaaacca gaatcgtctg cagcgaaaaa
     6661 cagcgcaaaa ggatcgaaac ggtcatagcc gaggccctgt tcggaccggc ggaggtggac
     6721 ccggttttgg aatttgcctg cggggaatat acgccgggat ttaccgatct caaagcggaa
     6781 atgagctatt tcaggaagtc catgatcgag ggacgctgtg ctgaccttgg cgacctcatc
     6841 gaattctgtg tgccggaccg gcgcgggaaa gcggcgtttg gagaaacgga ggtgcggttc
     6901 gaccggcgcg ggaatatccg cgtttacgat cggggaaaga agattgcctc gtttggcggg
     6961 gacgctcccg tgatggtccg caagacccct ctggcaagag tcagcctcgg ctttcagccg
     7021 cccatattcg gcgtcacgac cctcggcgcc ggacacggct tcgatcccgg cgccgacaca
     7081 tcgggcctga tcctctgggt gaaccgtcgc gggattatgg tggacccgcc cgtcaattcc
     7141 accgaaaaac tggccgctct cggcgtcagc ccgaagcgga tcgacaaaat cattttgacg
     7201 cactgccatg ccgatcacga tgcgggaact cttcagaaaa tattccagga ggggaaaatt
     7261 cacctgtaca ccacacgcac catttacggg agcttcatga agaaggccgc ggccctgacc
     7321 ggcattgagg cgggcctgct cgaacggctg gtggattttt atcccgtctg catcggaaag
     7381 cccgtgatca tcggcggggg agcctttcat ttccattaca ccctccatac gattccgacc
     7441 atcgccattc aggcgtctct gtccggaaaa agcctggtgt acagttcgga tacgatgaac
     7501 gatccgaact ttattcaaca gttgtatgcc gacggcgttt tgacgaaaaa ccgccgtgac
     7561 tttcttctga attttccgtg ggaccgggac ctggtgttcc atgaggcggg ggttccgccc
     7621 attcacacgc cgatgagcta tctgtgcagt ctgccggaag acgttcgcaa gcggatgtat
     7681 ctggtccatg taaatccgga ttccgttccc gatggctgcg gattgaggat cgctcccacg
     7741 gggctcgcga acaccgtgga tctgggcgct tctccgatgc cccgtgagga ggccgtcgaa
     7801 atgctggagg ccttctccgg cgttgaatgg ttcggccaga tgccgctcac gaaggcgaga
     7861 gaatttctgc tggccgccag tgtgacgcac caccgggctt ccgacattat tttcagcaag
     7921 ggagatgcgg gggaggcctt ctgcattgtc atgagcggcg agattgatat tgttttggag
     7981 gacaggatcc tcacgacgta tgatgccggc gggtatttcg gagaaaaatg cctgttttcc
     8041 gatgaagccc ggacggcaac ggccagggcg aaaacggacg tgaagcttct ttccattcgg
     8101 aaggaggaaa tgctgaacct gattcgggga accgccgcgg aaagcctgct tcgtcagatt
     8161 gccgattttc agaatgtgga attgcgcgac gccgtgaaag acaaccagat tctcgattcg
     8221 ctttcctcca cgcaaaaaac ccggcttcac gggctgatcc gtccttttcc cggcaccctg
     8281 cacacagggg acgttatcgc ctccaaaggg tcggacgccc gatgcgtcta tatgatccgg
     8341 gagggccgcg tcgatgttta ccatcgccgg tcacgcatcg cgacattgtc cagaggagac
     8401 ctgttcggcg tgaaaaaaat cttccgcccg aaagagaaaa acttttcatt tgtcgcaggg
     8461 gaaaagaccg ctctgtattt tattgaacaa gacgggctca ggcgctacat ggaagacaat
     8521 ccgggcgtgt acgtcaagct gtaccacagc ccctattgaa gcggcgggcg gcatcgcgaa
     8581 agcaggcggg ccgacggacg ggaaaccgtg gtccggctcc gccggaagac attgttgatg
     8641 tttcaggaat cgaaagggga aaggcttcat gcaatcatcg gacattcagc gtttgttcca
     8701 cgagcacggc cggaaagtcg ttctgcagga gggtgacgtg ttgttttgcc agggagagga
     8761 gagcgacgcg gtgtattttg tggccggagg acgtctggga gtcttcgcgg cgcagagctc
     8821 gtcggaagat gttcagatca acacgatcga agccggagag atggtgggtg agctgggagc
     8881 catcacccgt cacccgcgta ccgccacgct gaaagccgcc tcgccgcggg tggagctttt
     8941 atccatcgcg acgcccgatt tccgggcgct cctgtccgac agcctgtccc tggtcgaggc
     9001 catgaccctg accaaccggg agcatttgat ggacgcggac agggcgcgaa ttcaattcgg
     9061 aacctccaac cttcaaatgc ggaagcgcgt tgccagtctg gggcaggaga aggcgcagct
     9121 gcaggagctc ctgcagctgc gcgaagagct ggaagccatg gtggtgcatg atttgcgaaa
     9181 tccgctcaac accgtcatca tggccctcgg cctcgtggag cgcatgaagg atgaagtgaa
     9241 agatcccgag accttcgcgc tggccatgcg cctggcgaag ggagccgcga accgcatgaa
     9301 caggctgatt gccacgctgc tggacatcgc ccggctggag gccgggaaac tggcgctgag
     9361 catcgcgcag gttgatctga atacgctctt tgaagaagtg attgaaatgg aaaaggcggg
     9421 agtgacgaaa tccgttgaga ttaaggcggc tgcggttccg gggctgacgg taaaagccga
     9481 ccgggaggtg ttgtggcgcg tgatgtccaa tctgctgggc aatgccctga aattcgcgcc
     9541 ggaagcgagc accatcgtgg ttgaggcaca gcgcggagat gaccggaaga ttctcatcag
     9601 cgtgacggac gcggggccgg gcattccgga aggtgagcgc gaaagaatct ttgaaaagtt
     9661 taccagggtc aagaacgcgg acggcggagc ttacagcggg acgggtctgg gcctgacctt
     9721 ttgccgaatg gccgtggaag cccacggcgg ctgcatctgg gttgaggagg ggccttcggg
     9781 cgtcggaagc cgcttctgct ttcagcttcc ccaggattaa tccgcctgcc ccgggcggcc
     9841 gggttaccgg acggtgatct ccacccttcg atttctgggc tccggcgtgt tgtccggcgt
     9901 gggaatgagc agattccttt cgccgtggga ctcgatcgtc aggtcatgca cttgaatgcc
     9961 cgcgtcgcgg atgatttgcg ccacggtcct cgcccgctgc agagagatct gttcattgta
    10021 atctctgttt cccatggtgt cggtatggcc gatgacggat acgtccggcg cgggatattt
    10081 cattgccgcc tccacgatgg acgggatcag ggcctttgag tcgtttgtca gcagcgctcc
    10141 gcccgcttcg aagtacagca tgaaactgac cggcagcggc ggctgggcgg ccagcgccac
    10201 ggcgaagtct ttctgaatct tctgttcatc caccaaaaag ggcttttccg atttgccgtc
    10261 cagcgaggcg gcggttcggg gctggttcag aagcgtttcc tttttttcac cgctgaccgc
    10321 cagctttccc gtcgttccgt cctgattttc catcagaacg acataagact tgggtgaaca
    10381 gccggccagc gccagccaca cggcaagaag caccagacct gcctttgcaa aagccttcat
    10441 gcgatggtcc tcctgatgtt acggctccac tttgaccaga aagcgggttc ctcggcatcc
    10501 gatcacggcg gccggcgtct tcatttggac ggcctccggc ttgagtttgg cgatcacgcc
    10561 cgaaataacc tgcagggtgc ctttcgtcat gctggtgccg aatttcagcc ggtctttccc
    10621 cggttcatag aggtattcat ccaccgtcag ctccgtgtcc ggcccgaacg acattacagt
    10681 attgtccctg aaagtgatgc ccagagatcc gtccggtccg gttttcaata ggttgcccat
    10741 ctgaatcggc gtgccgaccc gggcgctgac ggcctttccg gcgtcgatca cttccgcttt
    10801 gcctgaagcg gttttgacgt agccgatggt gtccgcggcc caggcgggcg tcagagtcca
    10861 aaacaatgca ataaagactg ccgcaaatcg cttcatggta tttcctcctt ggcaacaggt
    10921 tcggttactt ttcttccaga atgatcgtat cacccttttt ccccgagcgc aaccggtccc
    10981 ggtggagacg gaccagttcg tcggccggtc tttccgcggc cagccgctcg aacgcttcga
    11041 gggcgagcag ctcccgcctt ttgaggagtt caaaggcctt ctcgtaagcg gtgtcccgtt
    11101 ccggctttcc gccgtttgcc tctggcgccg cgaggggttc atagacgctg atgggctgtg
    11161 ttttgccttt cagcaccagg gaggctgcgg gccggacgac ggcttcggga cagccttgaa
    11221 gcgtcgcttc cgacacgcag atcagcgtgc ccaggtattt gttcgcgcct tccaggcggg
    11281 aggccgtatt gacggcgtct cccagcgccc ggtagtcaaa cagggttttg ccgccgaaat
    11341 ttccaacaat gacctcgcct gagtggacgc cgatgcgggt ctgtcccaac tcgatgcctt
    11401 ttgcccggca gtccgccgcg tagccgaagg caaaggcatg catttccagt gcgcacgaca
    11461 gggcccgggc gcggtggtcc ggctgcgcga ccggcgccga aaacatgatc gcgacggaat
    11521 ctcccacgat ccggtccagt gtcccgcccc ggccaaaggc gatggtcacc atcgcgtcca
    11581 gataggcgtt gagaaccgaa accgcgtccg cggggtccat cttttccatc aggcctgtaa
    11641 acccggcaag gtcggtgaaa acaaaactgc attcctgccg ccgccctccc agctcgagct
    11701 ggtcggggtg gcggacaaga taatcgacgc ggttggggga aatgtagcgc gaaaaagcct
    11761 cgcgcaccca ccggtggcgt ctttcgctgg tccggtggtg gatgatgctg cccagaatga
    11821 acgcggccag cagcgccaga cccggcgtta cggggtccag aagcaggtgg tatcggacaa
    11881 aagcaatcca cgcgccccat ccggcggcga aaagaatcgc ggcggtgatg caggccgaga
    11941 cgagcgcggg agccgaaagc gcaatgattc cgactgcgag ccctccggcc agaatggtca
    12001 ggccctcgat ggccgccgcc caggaaggcc ttgaaatata cgtttttgtc aaaatctgtt
    12061 ccagggcctg ggcgtgcgct tccacgccgg gcatcaccgt tcccatcgga ctgaaccgga
    12121 tgtccatgag cccctgcgcc gaggcgccga cc
//
LOCUS       DAOSIM010000039        11677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_5494_length_11677_cov_8.478747, whole genome shotgun sequence.
ACCESSION   DAOSIM010000039 DAOSIM010000000
VERSION     DAOSIM010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 11677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11677
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5494_length_11677_cov_8.478747"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="PLR47_03075"
     CDS             <1..1246
                     /locus_tag="PLR47_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOG11951.1"
                     /translation="KANNYQSLHTAVIGPYGERVEIQIRTRAMHEWAEKGIAAHWRYK
                     EGRAFQDHEDDQIKKLREALEIQQDIKNPKEFMSNLKLALFPEEVYVFTPHGDVKSFP
                     KGATPVDFAYSIHTDVGNQCTGAKVNRNIVPLRYQLQNGDMVEIITQTGHHPSKDWLK
                     FVVTSRAISKIRNWINAEEKNRSLVLGKDLLEKEFKRNNLKFSSYLKSDEIRKLLKDY
                     SLLTLDDLISQVGCGRISPRQVVNHFVVQEAPKEAEKPSEKEKKKPAAAPSLGISLTG
                     IDDVMVRFAKCCNPIPGDEITGYISHGRGITVHTQNCPHLQKLDIERIVEVNWDLRQK
                     NAYPVHIKVVCTDRKGVLTEVSSVITSLDVNISYAQIETTDMIANCIFIIDVYDTRQL
                     NAVFSAIRQLKIVRSIERIRKA"
     gene            1353..2033
                     /locus_tag="PLR47_03080"
     CDS             1353..2033
                     /locus_tag="PLR47_03080"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOG11952.1"
                     /translation="MLQTWQKYTVFLFSVLALCLAVFPGTAQTAKHIVVIDAAHGGDD
                     AGVLGVDGAAEKDVTLAIALALQKELAKGGNMEVALTRNTDRTLSLDERKKAISKINP
                     ALVLSLHANGGFGKNATGFELYYPGFSKAGAANKTAKAAAPDYGYLNDTVKLARLVQK
                     NLDSLFPRQRRGLREAGIPLAEGLALPVLVVEIGFITNADGKKKLTSVKTQAEIAGAL
                     AKSIQAFF"
     gene            2044..2775
                     /gene="rph"
                     /locus_tag="PLR47_03085"
     CDS             2044..2775
                     /gene="rph"
                     /locus_tag="PLR47_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOG11953.1"
                     /translation="MFKRSADRKWDELRPIAIANRYLKNVPGSVMVEFGNTKVLCAAS
                     LDEKTAPFLKNTGRGWLTAEYAMLPMSTSTRSARESARGKIGGRTHEIQRLIGRSLRA
                     VTDLAAFGERTIYIDCDVIQADGGTRTASITGAFVALVELFKDMKERGIVREIPVADF
                     VSAVSVGMLENQVLLDLEYEEDSRADVDMNFVMTGSGLFIEVQGTAEHAPFGKDRLDA
                     MTALAVEGIGQIIARQKEITGDWRS"
     gene            2772..3368
                     /locus_tag="PLR47_03090"
     CDS             2772..3368
                     /locus_tag="PLR47_03090"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HOG11954.1"
                     /translation="MRIVFATKNEGKVREISEMMEGTGIEMVSLNHFAALPEIVEDGA
                     TYLENALKKARIVSEFLGETVLADDSGLQVDALQGEPGVYSARYAGEGATDEENNAKL
                     LAKLKEVPAEKRTASFFCALVLYRSGGVFHSFEAEWRGRIIDEGRGANGFGYDPIFLD
                     SNFNRTAAELPSEIKNKVSHRGQAFAKFKAWLEAGRSV"
     gene            3374..3450
                     /locus_tag="PLR47_03095"
     tRNA            3374..3450
                     /locus_tag="PLR47_03095"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3408..3410,aa:Pro,seq:tgg)
     gene            complement(3477..4067)
                     /locus_tag="PLR47_03100"
     CDS             complement(3477..4067)
                     /locus_tag="PLR47_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOG11955.1"
                     /translation="MMDNTSTQMSVPEIRDHLSIIADTCLRFQMISLTRQLEAAQALL
                     SENPPIDIAVLGQFKAGKSSFLNNLIGHKVLPVGAIPVTTAITRLQYGSSERAFVRHF
                     NGQTTEIPLADVVRFTSEAQNPGNEKNVAMVDIELPSMQEYPGLRLVDTPGLGSVYQY
                     HQSTSEEWLPSVGTAILAVSFNRPLRLAELLKSKRL"
     regulatory      complement(4091..4155)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(4245..5075)
                     /locus_tag="PLR47_03105"
     CDS             complement(4245..5075)
                     /locus_tag="PLR47_03105"
                     /inference="COORDINATES: protein motif:HMM:NF024884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HOG11956.1"
                     /translation="MKSLPAILLMLILTTILNIFLEKFIQPSSLVFVYLVPAIAGAIY
                     FGIWASVLSFTAGFLIFDFSFVKPYHTLHIANPQDVYNVIVYFTSAILMTYLISMVHR
                     QNVFLKDRLNRVSLIEDMSRDLLLLTPIGQTYSIQGMTLLLKTAVWSQLGQLALRYTK
                     MILDVPLLVFFREDDGSLKLWAKSNVDLEITEQDHAAASWTLDNGEVSGSGTYTYSDN
                     PFYFIPMKHLEETIGVIGILYDSKDLFPEQRRLLGTVSNLVTIVATIWMSVKAEPEQH
                     "
     gene            complement(5203..7179)
                     /locus_tag="PLR47_03110"
     CDS             complement(5203..7179)
                     /locus_tag="PLR47_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOG11957.1"
                     /translation="MNTLDSRHEKNIRHRLWQKLVGKPRNINEPSIFHKLSLIPLLAW
                     IGLGADGLSSSSYGPEEAYKALGAHTYIAVFIGLATAFTVFIIAYTYSRIIEHFPQGG
                     GGYIVSTKTISNSAGVISGCALIVDYVLTITVSLVACGDAIFSFLPITFQNYKIIFVA
                     FLILMLVTMNLRGVKESIIVLAPIFIVFVLTHVLLITYGIAHHADRFGWVVNQFHGSL
                     RQDIGTIGFIGILAIFLRAFSLGGGTYTGIEAVSNGIQIMREPRVQTGKRTMLYMAVS
                     LAFTAGGLLICYALLQINPQEGKTLNSILADEVFRNWPLSGGIVLITVFSEGALLLVA
                     AQAGFVDGPRVMANMATDFWLPRRFAMLSERLTMQNGILLMGAAAMMLLFYTHGSISA
                     LIVMYSINVFLTFSLSEFGMTLFSFRKRNQDEKWKRHIFIHVVAFILCIIILFVTIYE
                     KFGEGGWITLIITSGLIALCYLIKKHYLYVRGKALKLEDILQKVHPGKKFNNDPLNPK
                     DPTAILLVSGFNGFGLHTWFSINRKFPHFYKNFIFISIAEVDQASFKGSAEMEELRIS
                     VENGLKKYVDLVRSFGVPADYKMDAGTDVIETATQMCQSLAKEFPKSMVFTGKLIFHE
                     EHFYQRALHNETAYAIQRRLQWDGIESVIMPIRV"
     regulatory      complement(7480..7617)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(7828..8892)
                     /locus_tag="PLR47_03115"
     CDS             complement(7828..8892)
                     /locus_tag="PLR47_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOG11958.1"
                     /translation="MKVLALNSSSRTGDVSKTEIILGDLLQGMREAGAEVEVVELRRK
                     KIRYCIGCFTCWTKTPGVCIHKDDMTREIFPKYRDSDLVVLATPLFHFTVNAIMKTFI
                     ERTLPIALPFFEKQDGATRHPYRHKAPPVVVLSVAGFPEEGVFDALKFYARFLFRDHL
                     AAEIYRTSSEMFLHSTGSRKVSEVREALIQGGRELVQSMKISPETLKRIHKPITTFEE
                     MAPLGNLMWQTCIDEGVTLGEAQKKKIIPRPDSIENFLMLMRFAFKARKAADAKMAVQ
                     FNFSGQAAGECYFIVENGTFRTDLGTADHPDLIIESPFEVWMDIMTQKADGQKLFMEQ
                     KYSARGDITALMRFKDLFGN"
     gene            complement(8914..10053)
                     /locus_tag="PLR47_03120"
     CDS             complement(8914..10053)
                     /locus_tag="PLR47_03120"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOG11959.1"
                     /translation="MIRFHGRFLWITLLLIGMVCLLAAHAQAKAPLAAGQAILEAIRI
                     QGPLDFCGERVPLDDPDVRERLERELLVSLDNSDDIILWLKRANRYFPDVEKVLKAQS
                     LPDDLKYITIAESSLRPLAFSNKGAAGYWQFIESTGKRYGLEISGDIDERRNVHRATQ
                     AAIAYLKELHNLFGSWTLAAAAYNMGEDGLQAELLIQKVGNYYQLYLNQETQRYVFRI
                     LSAKIIMSHPERFGYSLSKSDLYAPRRTETVEIRVTEPVPLHIIAQAADTHFKIIKDL
                     NPQIKYYSLPAGTHAVHIPAGQAKGFYERYDKALADWIALKNDSVYVVKKGDTLTGIA
                     KRFNVPYRALTIWNPGGGRKLSPGDKLYIFSDSLKEGQSHEQANP"
     gene            complement(10090..10443)
                     /locus_tag="PLR47_03125"
     CDS             complement(10090..10443)
                     /locus_tag="PLR47_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11960.1"
                     /translation="MKKDVIVLFAVCVIFCFSGGVSFAAGESSSSGKSVTKPIKETAK
                     EVKQGTVKVYKESKDAIVRDAKAMKEEVPKDVKEAKKEAVQKAKDVKKSAASEWKEIR
                     DNMANPTLTTNPEKK"
     gene            complement(10546..>11677)
                     /gene="eutB"
                     /locus_tag="PLR47_03130"
     CDS             complement(10546..>11677)
                     /gene="eutB"
                     /locus_tag="PLR47_03130"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF017768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HOG11961.1"
                     /translation="RCCLEDIVMGKLHGLTIGLDICSTLHMDVSLDDLDWCQDRIMPA
                     NPAYLMALPTKNDPMLGYLTTAFQDHVRLREKFNYKVNDDMQRFFQRLEILDAAGNPA
                     SHFGDPLWVWYAYRLAKGDRRSRAAIYREGKKKMQEVRNRGVYLAQGYDGRPWKMEAK
                     LDREIRELYADSKKCIWVELSPSFIASLPNAVVLKSRSKDRTDYILHPQTGEVLEDAS
                     WQILKEMKAKRGDAVQVQIIVSDGLNAHAVMDPGHLAPYLSHLEKGLKEKHRTLAPEL
                     LVLQGGRVRAGYRIGEVLFGDKPNAREHKAIIHIIGERPGTMHHTFSAYITAPPAAVW
                     TRAGIDHNITRVVSGVADTALRPDLAAAQTVKILDDLWKGDV"
BASE COUNT         2767 a         3018 c         3162 g         2730 t
ORIGIN      
        1 caaggccaac aactatcagt cgctgcatac ggcggtgatc ggcccctacg gcgagcgggt
       61 cgaaatccag atccggaccc gcgcgatgca tgaatgggcc gaaaagggca ttgccgccca
      121 ctggcgctac aaggaagggc gggcttttca ggaccacgag gacgatcaga tcaagaagct
      181 ccgcgaggcg ctggaaattc agcaggacat caagaacccg aaggagttca tgtccaacct
      241 taaactggcg ctcttcccgg aagaagttta cgttttcacg ccgcacggcg acgttaaatc
      301 cttccccaaa ggcgccacgc ccgtggactt cgcctacagc atccacaccg atgtcggcaa
      361 ccagtgcacg ggcgccaagg tgaaccgcaa tatcgttccg ctgcgctatc aactgcagaa
      421 cggcgacatg gtggagatca tcacccagac cggccatcat cccagcaagg actggctgaa
      481 gttcgtcgtt acctcccggg ccatttccaa aatccgcaac tggatcaacg cggaggagaa
      541 gaaccgttcg ctcgtgctcg gcaaggacct gctggaaaag gaattcaaaa gaaacaacct
      601 gaaattctcc agctatctca aatccgacga aatcaggaaa ctcctgaagg actactcgct
      661 cttgacgctg gacgacctga tttcgcaggt gggctgcgga cgcatttcgc cccgccaggt
      721 cgtcaatcat tttgtggttc aggaagcgcc gaaggaagcg gagaagcctt cggaaaagga
      781 aaagaaaaaa ccggccgccg ctccttcgct gggcatcagc cttaccggca tcgacgacgt
      841 catggtgcgg ttcgccaaat gctgcaatcc cattcccggc gatgagatca ccggctacat
      901 ctcgcacgga cgcggcatca ccgttcacac gcagaattgt ccgcacctgc agaagctcga
      961 catcgaaagg atcgtggagg tcaactggga tctccgccag aaaaacgcct atcccgttca
     1021 catcaaagtc gtctgcaccg accgcaaggg cgttttaacg gaagtgagtt cggtcatcac
     1081 ctcactggac gtcaacatca gctatgccca gatcgaaacg acggacatga ttgccaactg
     1141 catcttcatc atcgatgttt acgatacccg gcagctcaac gccgttttct ccgcgattcg
     1201 ccagttgaag atcgtccggt ccatcgaaag gatccgcaag gcctgatggc ctcttcccgt
     1261 tccatttgtt ggttgacaaa ccatcggact ttgctataag caggcccaac tccggtttgc
     1321 gcggggaaga acatcattca caggtgaaaa aaatgcttca aacatggcaa aagtatactg
     1381 tttttctttt ttctgttctg gcgctgtgcc ttgcggtttt tccggggacc gcccagacgg
     1441 caaagcacat cgtggtgatc gacgcggctc acggcggcga cgacgcaggg gtcctcgggg
     1501 ttgacggcgc cgccgaaaag gacgtgacac tggccattgc cctggccctg cagaaggaat
     1561 tggccaaagg gggcaatatg gaagtggcgc tgacccggaa tacggatcga acgctgtcgc
     1621 tggacgaacg caagaaagcc atttcgaaaa tcaatccagc cctggtgctg agcctgcacg
     1681 ccaatggcgg ctttggcaaa aacgccacgg ggtttgagct gtattatccc ggcttttcaa
     1741 aggccggcgc ggcaaacaag acggccaagg ccgccgctcc ggactacggg tatttgaacg
     1801 acaccgtgaa gctggcgcgc ctggttcaga aaaatctcga cagcctcttt ccccgccagc
     1861 gccgtgggct tcgggaagcg gggattccgc tggcggaagg attggccttg cccgtgctgg
     1921 tcgtggagat cggttttatc acgaacgccg atggaaaaaa gaagctgacc tccgtaaaaa
     1981 cgcaggcgga aattgccgga gccctggcga aaagcatcca agcgttcttt taggaggcct
     2041 gtgttgttca aaagaagcgc agacagaaaa tgggacgagc tgcggccgat tgccatcgcc
     2101 aaccggtatc tgaaaaatgt tcccggctcc gtgatggtgg agttcggcaa caccaaagtg
     2161 ctctgcgccg cgtcgctgga cgagaaaacg gcgccctttt taaaaaatac cggcaggggc
     2221 tggctgaccg cggaatatgc catgctgccc atgtccacgt ccacccgctc ggcgcgggaa
     2281 tccgcacgcg gaaaaatcgg cggcaggacg catgaaattc agagactcat cggtcggtcg
     2341 cttcgcgccg tcacggacct tgccgctttc ggcgaaagaa ccatctacat cgattgcgac
     2401 gtcattcagg cggacggcgg aacccgcacc gcatccatta ccggggcgtt cgtcgccctg
     2461 gtggagcttt ttaaggacat gaaggagcgg gggatcgtcc gggaaattcc cgttgcggat
     2521 ttcgtttcgg ccgtgagcgt cggtatgctg gaaaatcagg ttctgctgga cctggaatat
     2581 gaagaagact cgcgggcgga tgtggacatg aattttgtca tgaccggatc cggccttttc
     2641 attgaagttc agggcacggc cgagcacgct ccgttcggaa aagaccggct ggatgccatg
     2701 accgcgctgg ccgtggaggg cattgggcag atcatcgccc gccagaagga aatcacgggc
     2761 gactggcgat catgagaatc gtgtttgcga cgaaaaatga gggcaaagtc cgggaaatct
     2821 cagagatgat ggaagggacc ggcattgaaa tggtttctct caatcatttt gcggcgctgc
     2881 ccgagatcgt ggaagatggg gcgacctatc tggaaaacgc tttgaaaaaa gcaagaatcg
     2941 tttccgaatt tttgggtgaa accgtactgg ccgacgattc cggcctgcag gtggatgccc
     3001 ttcaaggcga acccggcgtt tactccgcgc gctacgcggg cgagggcgcc acggacgaag
     3061 aaaacaacgc caaactcctg gcgaaattga aagaagttcc cgcggagaaa agaacggcct
     3121 cctttttttg cgccctggtt ctataccggt ccgggggcgt cttccattca tttgaagccg
     3181 aatggcgggg ccggatcatt gatgagggcc ggggcgcgaa cggtttcggt tatgatccga
     3241 ttttcctgga ttcaaacttc aacaggacgg cggcggaatt gccttcggag attaaaaaca
     3301 aggtcagcca ccgcggccag gcgtttgcaa agttcaaagc ctggctggag gccggccggt
     3361 ccgtataaaa aaacggggcg tagcgcagcc tggtagcgcg cttgctttgg gagcaagatg
     3421 tcgggtgttc aaatcacccc gccccgacca gtcccttcaa gaggttatgc cttccctcat
     3481 aacctctttg acttcagcag ttcggccaat ctcagcggac ggttgaagct caccgccagg
     3541 atggccgtgc cgacggaggg aagccattct tccgacgtgg actggtggta ttgatacacg
     3601 ctgcccagcc ccggagtgtc cacgagacga aggccgggat attcctgcat ggacggcagt
     3661 tcgatatcca ccatcgccac gtttttttcg ttcccggggt tttgcgcttc ggacgtgaac
     3721 cggacaacat cggcaagcgg gatttccgtg gtctgaccgt tgaaatgccg aacgaaagcc
     3781 cgttcgctgc ttccatattg caggcgggtg atggccgtgg tcacaggaat ggcgcctacg
     3841 ggcagcactt tgtggccgat caggttattt aaaaaagaac ttttccccgc cttgaactgc
     3901 cccaaaaccg cgatgtcgat ggggggattt tctgaaagaa gcgcctgcgc ggcctccagt
     3961 tgccgggtca gggaaatcat ctggaaacgc aggcacgtat ccgcaatgat tgataaatga
     4021 tcgcggattt cgggaaccga catttgggtt gaagtgttgt ccatcataaa aaaaactccc
     4081 acagacttaa gaatctgcag gagttatcag caaaaattgc ggttgttagg ggtgaactcc
     4141 atcacccatt gtcaggacgg ccaatatttg cagcgaacct attttaaaaa taacgggttg
     4201 tcaaccggat tattgaccgg ctcatgatgc ccgctgttcg agattcaatg ctgttccggc
     4261 tcagctttca cgctcatcca tatcgtggcg acgatggtta ccagattgga gacggttccc
     4321 aaaagacggc gttgctccgg aaaaagatct ttagaatcat ataagattcc aatgacgcca
     4381 atggtttcct caagatgctt catcggaata aagtagaatg gattgtcgga gtaggtatag
     4441 gtccctgaac cggaaacctc cccattatcc aatgtccagg atgccgcggc atgatcctgt
     4501 tcggtaattt ccagatccac gttgcttttt gcccaaagct tgagactgcc gtcatcttcc
     4561 cggaagaata caagcagcgg cacatccaaa atcatttttg tatatcgcaa ggcaagctgt
     4621 cccaattggc tccaaacggc tgttttcagc aagagcgtca tgccttggat ggaatacgtt
     4681 tgtccgatag gggtaagcag caataaatct ctgctcatgt cttcgatgag ggacacacgg
     4741 ttcagtctgt ctttcaggaa tacattctga cggtgaacca tgctgatcag atacgtcatc
     4801 agaatggcgc tggtgaaata aacgatgaca ttatagacgt cctgcggatt ggcgatatga
     4861 agagtgtgat acggtttcac aaaagaaaaa tcgaagataa ggaatccggc ggtaaaagat
     4921 aataccgaag cccagattcc aaaataaatc gcgcctgcga tcgcgggaac caggtaaaca
     4981 aaaaccaacg aagacggttg tatgaatttt tccagaaata tattgagaat ggtggtgaga
     5041 atcaacatca ggagaatggc cggcaaggat ttcatttttt ccattgtttc tttgctgagg
     5101 actttgcctt ggtcgaaaaa cggctctgga gcgcgacttt gatctatttc ctgcataatc
     5161 gttgacctcc ttttaataaa ttccgaagtc tccttcataa gatcaaaccc gaatgggcat
     5221 aatgaccgac tctattccat cccattgtaa gcggcgctga atcgcgtaag ctgtttcgtt
     5281 gtggagcgcc ctctggtaaa aatgctcctc gtgaaaaatc agtttcccgg taaaaaccat
     5341 ggactttgga aattcttttg ccagcgattg acacatttga gtcgcggttt caatgacatc
     5401 cgtgccggcg tccattttgt aatcggccgg cacgccgaag gaacggacaa gatcgacata
     5461 ttttttcaaa ccattctcaa cggagattct caattcttcc atttccgctg atcctttgaa
     5521 ggatgcctga tccacttcgg caatggaaat gaaaataaag tttttataga aatgcgggaa
     5581 tttacgattg atcgaaaacc acgtatgcag accgaaaccg ttgaaaccgc tgaccagcaa
     5641 tatcgccgta gggtctttcg gattcaaagg atcgttgttg aatttcttgc cgggatgaac
     5701 tttctgtaaa atgtcttcca acttgagcgc ttttccccgg acataaagat aatgtttctt
     5761 gatcaaatag cataaggcga tcaaaccgga cgtgatgatt agggtgatcc agccgccttc
     5821 gccaaacttt tcgtaaatgg tcacaaagag gatgatgata caaagaataa aagcaacaac
     5881 gtgaataaaa atatgccttt tccatttttc atcctggttc cttttgcgaa aagaaaaaag
     5941 ggtcatgcca aattcagaaa gggaaaaggt tagaaaaaca ttgatggaat acatgacaat
     6001 aagggcggaa atggaaccgt gcgtataaaa caaaagcatc atggccgcag cacccattaa
     6061 aaggatgccg ttttgcatgg tcagtctttc ggaaagcatc gcaaaacggc ggggaagcca
     6121 gaaatcggtc gccatattcg ccatcactcg cgggccgtcg acaaaccccg cctgagccgc
     6181 cacgagaagc aatgctccct cggaaaaaac agtgatcaat acgatgccgc cggataaagg
     6241 ccagttgcgg aagacttcgt cagccagaat cgaattgagc gtcttgccct cctgtggatt
     6301 gatttggaga agagcataac agatcaacaa gccgccggcc gtaaaggcaa gagataccgc
     6361 catgtaaagc atcgttcgtt ttccggtctg aacccgcggt tcacgcataa tctggatgcc
     6421 gttggatacg gcttctattc ccgtgtaggt tccgccaccc agagaaaatg ctctcaaaaa
     6481 tatcgccagg atcccgatga aaccaatggt cccgatgtcc tgccgaagac ttccgtgaaa
     6541 ttgatttacg acccatccaa atcgatcggc atgatgcgct atcccgtatg taatgagcag
     6601 aacgtgggtc aaaacaaaaa caataaagat cggcgccaga acgatgatgg attctttaac
     6661 gccccgcaga ttcatggtca ccagcatcag gatgagaaag gccacaaaga tgattttgta
     6721 gttttgaaat gtgatgggca aaaaactgaa aatagcatcg ccgcaggcaa ccagcgaaac
     6781 ggtaatggtc agcacgtagt ccacaatcaa agcgcagccc gagatcacgc cggcgctgtt
     6841 gctgatggtt ttggtggaaa caatgtatcc cccgccgcct tgcggaaaat gttcaataat
     6901 gcgtgaatac gtgtacgcaa tgataaaaac cgtgaaggcc gtggccaacc cgatgaaaac
     6961 cgcgatgtag gtgtgggctc ccagcgcttt gtaggcttct tccggtccat aggacgaaga
     7021 tgacaaacca tccgcgccca ggccgatcca tgccaggagc ggaatgagag acaatttatg
     7081 aaaaatggat ggttcgttga tgttgcgcgg cttgcccaca agcttttgcc atagcctgtg
     7141 ccttatgttt ttttcatgcc tggaatctaa tgtattcatg atgacgtaat ttctttggtc
     7201 tgcatccggt tcatggtgcg ttgcaggatt gcgcctcaca atacacgaca cgtttataaa
     7261 aaaaccgtta tccacatcgc gtcttggaga gaagaaaagg gaaataaaag ctctcctcgg
     7321 caagttgcgg agaatcttcg atcagggagg tgatattgtt ttgatattgc tcgatgctgg
     7381 attcaaaaac ggcaatggct tgatttttgt ttcagcggtt tcagatgaag gacacagacg
     7441 aagtatcggc aaagccgacg aaagaagagt aaacattctc cttttcctcc cacatgccga
     7501 cgaggttagc tgacgggctt ggacttggaa gtatcctatt ctttagaaat tcgccccgaa
     7561 ataccggttc ccccgtacct atcatttcat tttgatagat ttaggcgtcg ttcaatattt
     7621 gactgttagc tacgcctctt tataacaact gtcaagagat tatttgcgat acaattttgt
     7681 taagtatttg gaacaattat gtatttctta accacgcgcc gatgccttta tccggataat
     7741 ttcggctccg tttggaggaa tcccaatgaa tccatccgtt ttgaattcat tttgactccc
     7801 cgtgcctcaa gtagatcgtt ctggcgctta attgccgaag agatccttga agcgcatgag
     7861 ggccgtgatg tccccccggg cggaatactt ttgttccatg aacaatttct gcccgtccgc
     7921 tttctgagtc atgatgtcca tccagacctc aaagggcgat tcaatgatca ggtcgggatg
     7981 gtccgccgtt cccaggtcgg tccggaaggt tccgttttcg acgataaaat aacattcccc
     8041 cgccgcctgc ccggaaaaat tgaactgtac ggccatcttc gcgtctgcgg ctttgcgggc
     8101 tttgaaggcg aagcgcatca gcatcaggaa attttcgatg gagtcggggc gcggaattat
     8161 ttttttcttc tgggcttcgc ccagggttac gccttcgtca atgcaggtct gccacatcag
     8221 gtttcccagg ggcgccatct cttcaaaagt agtgatcggc ttgtggattc ttttcagggt
     8281 ttccggggag attttcatgg actgaaccag ctcccggccg ccctgaatca gcgcttcccg
     8341 aacctcggag actttcctgc tgccggttga atgcaaaaac atctcggagg atgtccggta
     8401 gatttccgcc gcgaggtgat cgcgaaacag gaatcgcgcg tagaatttca gcgcgtcaaa
     8461 aacgccttct tccggaaatc cggcgacgga aagaacgacg acgggcgggg ccttgtgacg
     8521 gtagggatgc cgggttgccc cgtcttgttt ttcaaaaaaa gggagcgcga tgggaagcgt
     8581 ccgctcgatg aaggttttca tgatggcgtt cacggtgaaa tggaaaagag gggtggccag
     8641 gaccaccaga tcgctgtccc ggtatttcgg gaaaatctcc cgggtcatat cgtctttgtg
     8701 gatgcaaacc ccgggggttt tggtccagca ggtaaagcat ccgatgcagt accggatttt
     8761 tttcctgcgc agttcgacga cctccacctc cgcgcccgct tcgcgcatgc cctggagcag
     8821 atcccccaga atgatttcgg tcttgctgac gtctcccgtc cgggagctgg aattaagagc
     8881 gagaactttc atgggttcct cctttgtcct gatttaagga ttcgcttgtt catggctttg
     8941 gccctctttc agggaatcgg aaaaaatata aagcttgtcg ccgggagaaa gcttcctgcc
     9001 gccgccgggg ttccagatag tcagggcccg atacggcacg ttgaatcgtt tggcaatgcc
     9061 cgtcagggtg tctccctttt tcacgacata tacggagtcg tttttcaggg cgatccagtc
     9121 cgccagcgct ttgtcgtagc gctcgtaaaa acctttcgcc tggcctgcgg gaatatggac
     9181 ggcatgagtc ccggcgggca gggaataata tttgatctgc gggttgaggt ctttgatgat
     9241 tttgaaatgc gtgtcggccg cctgggcgat gatgtggagg ggcacgggct cggttacccg
     9301 gatctccacc gtttcggtcc gccggggagc gtagaggtcc gatttggaca gcgaataccc
     9361 gaaccgttcc ggatgggaca tgatgatttt tgccgagagg attcggaaaa cgtatcgctg
     9421 cgtttcctga ttcaggtaaa gctggtagta gttgccgacc ttctggatga gcaattcggc
     9481 ctggaggccg tcttctccca tgttgtaggc cgccgccgcg agcgtccagg atccgaaaag
     9541 gttatggagc tctttgagat aagctatcgc cgcctgtgtg gccctgtgaa cgtttcttct
     9601 ttcatcgatg tcgccgctga tttccaggcc gtatcttttt cccgtgcttt cgatgaactg
     9661 ccagtatccc gcggcgccct tgttggagaa ggccagcggc cgaagggagc tttccgcgat
     9721 ggtgatatat ttcagatcgt cgggcaggga ttgcgctttc aggacttttt cgacgtcggg
     9781 aaagtagcgg ttggcgcgct tcagccagag gatgatgtcg tcgctgttgt ccagggacac
     9841 cagcagctca cgctccaggc gttcgcgcac atcgggatcg tcgagcggca cccgttcacc
     9901 gcaaaaatcc agcggcccct gaatccgaat ggcttccagt atagcctgtc cggcggcaag
     9961 cggcgctttg gcctgcgcgt gagccgccaa cagacagacc atgccgatca gaagcagggt
    10021 gatccaaaga aatcgtccat gaaatcgtat catcaaaacc agtcctttcc ggaatggatt
    10081 tattccggat tacttttttt cggggtttgt ggtcagtgtc ggattggcca tgttgtcgcg
    10141 gatctccttc cattccgaag cggcgctttt cttgacgtcc ttggctttct ggacagcctc
    10201 cttttttgct tccttcacgt ctttggggac ttcttccttc attgctttgg cgtcccgcac
    10261 aatcgcgtcc ttgctttcct tgtaaacttt aaccgttccc tgcttgacct cttttgctgt
    10321 ttccttgatc ggtttcgtga cgcttttgcc ggatgacgaa gattctcccg cggcgaagga
    10381 cacgccgccg ctgaagcaga agataacgca gacggcaaac aacacaatca catctttttt
    10441 catgacagga tctcctttat caataattta ttctttcgaa aaccttgaaa taactggatt
    10501 cccgcctgcg cgggaatgac aacattactg cttttatggg ttgattcaaa cgtctccttt
    10561 ccaaagatca tcgagaattt ttacggtttg ggcggcggca agatcagggc gaagggccgt
    10621 gtccgccacg ccggaaacca cccgggtgat gttgtggtcg atgccggccc tggtccagac
    10681 ggccgcgggc ggcgccgtga tatacgcgga aaacgtgtga tgcatggtgc cgggtcgctc
    10741 gccgataatg tgaatgatgg ctttgtgttc ccgggcgttt ggcttatccc cgaacaggac
    10801 ctcgccgatt cggtatccgg cccgtacgcg tccgccttga agcaccagga gttcaggggc
    10861 gagcgtccgg tgtttttcct tcaggccttt ttcaagatgg gacagatatg gcgccagatg
    10921 tccggggtcc atcacggcat gggcgttgag gccgtcggac acgatgatct gcacctggac
    10981 ggcatcgcct ctttttgctt tcatctcttt caggatttgc caggatgcat cctccaatac
    11041 ctctcccgtt tgcggatgca gaatgtaatc cgtcctgtcc tttgaacggc ttttgagcac
    11101 gacggcattc ggcagggaag cgatgaagga cggcgaaagc tccacccaga tgcatttttt
    11161 ggaatccgcg tacagctcgc ggatttcccg atccagcttc gcctccatct tccatggccg
    11221 gccgtcatag ccctgggcca gatagacacc gcggttccgt acctcctgca tctttttttt
    11281 gccctcgcgg tagatcgccg cgcgggagcg ccggtcccct ttcgcaaggc ggtatgcgta
    11341 ccagacccac agcgggtcgc cgaagtgcga ggccggattg cccgccgcgt cgaggatttc
    11401 cagacgctgg aagaagcgct gcatgtcatc gttgactttg taattgaatt tttcgcgcag
    11461 gcgcacgtgg tcctgaaagg ccgtggtgag atagcccagc atgggatcgt tcttggtcgg
    11521 cagcgccatg aggtaggccg gattggccgg catgatccgg tcctggcacc agtccagatc
    11581 atcgagagaa acgtccatgt ggagcgtgga gcagatgtcc aggccgatgg tgaggccgtg
    11641 cagcttgccc atgacaatgt cttccaggca gcagcgc
//
LOCUS       DAOSIM010000040        11586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_5548_length_11586_cov_7.463620, whole genome shotgun sequence.
ACCESSION   DAOSIM010000040 DAOSIM010000000
VERSION     DAOSIM010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 11586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11586
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5548_length_11586_cov_7.463620"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="PLR47_03135"
     CDS             <1..823
                     /locus_tag="PLR47_03135"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004396 - hexokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hexokinase family protein"
                     /protein_id="HOG11962.1"
                     /translation="SDALRRKRIEAVSVTALVNDTVGTLATKSYADSSCDMGVILGTG
                     TNACYPEKTGRLAKGLFPLPRQEMILNLEWGNFDKLDTNACDQALDRMTLNPGCQRME
                     KMVSGMYIGELVRLILLDMTDQGILLQEKHRPVLAREHSVSSEQMSLSALGEDFFAAI
                     GIPDVSDRDRDAAAHVCRIVSRRSARIAGAALAAVVTWMDAHLEKKHTIAIDGSLFEK
                     YPGYPENVRCVLEEFFACRADRISLCTTSDGSGIGAAIIAAVASFGQEQKHKPAR"
     gene            972..1814
                     /locus_tag="PLR47_03140"
     CDS             972..1814
                     /locus_tag="PLR47_03140"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOG11963.1"
                     /translation="MKTQSGNFKSQDGTSIYWQGWTPDDASRPKAVVQVIHGYAEHIG
                     RYGNVVGELLPAGYAVFGTDHRGHGKSEGKRGHVMSFQEFIDDEMQFRREVIRTKFSG
                     LPCFVLGHSMGSLIAMNLVEQNANEIQGLVLSGTGSRPGTDIPKILLTATKILSGLLP
                     SIHVKSPLPPEFISRDPEVVRAYVEDPLVYNVITPRLAHEMNRYVVIGAENAGKIQIP
                     VLIQLGSQDTAFSGQKELFEMVGAKDKTFKRYEGLKHEVYNELAHDRTRVLQDLRSWL
                     DAHV"
     gene            1950..3308
                     /locus_tag="PLR47_03145"
     CDS             1950..3308
                     /locus_tag="PLR47_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOG11964.1"
                     /translation="MKIQRIPKPLPFDPESGQNIILDSIADGVFTVDQDWRITSFNRA
                     AQEITRVSREEAVGQMCKDVLKADICEKNCCLRMTMKTGKPIINKKVNIIDADGRKLP
                     ISISTALLRDGKGKLLGAVETFRDISVEEDLRKAIQGKYSFEDIISKNHRMLRLFDIL
                     PDIAASSSTVLIEGESGTGKELVARAIHNLSPRKRQPFVAVNCSALPDTLLESELFGY
                     KAGAFTDAKKDKPGRFHAAENGTLFLDEIGEISPAMQVKLLRVLQEKTYEPLGTTRSV
                     EHNVRILAATNKNLEDLVRQNRFREDLFYRINVFKIHLPPLRERMEDIPLLIDHFIDR
                     FNILQKKNIEEISDDAMALLMAYRYPGNIRELANLIERAFILCKEGRIEKKHLPDFLF
                     AANTSPEGKTAASFRDLEADYLIDALRKNNGNRPQTARQLGIHRSTLYRKIKALKLSP
                     ND"
     gene            3502..3912
                     /locus_tag="PLR47_03150"
     CDS             3502..3912
                     /locus_tag="PLR47_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOG11965.1"
                     /translation="MKLALSVFKDCISTVFDSSDQLLILETEGVNGTKRTTVRIDATD
                     PAGRAARLRDQGVAVLICGAISRPMQMAIASLGIAVYPFIRGTVEEVLAAYQSNRLGQ
                     AVFSLPGCHGRGNGGNGRGRGMRCRWRQTGGHER"
     gene            3940..4488
                     /locus_tag="PLR47_03155"
     CDS             3940..4488
                     /locus_tag="PLR47_03155"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HOG11966.1"
                     /translation="MNSSIPKELFFTKGVGTHREQLHSFELALRNAGIEKCNLVQVSS
                     IMPPGCKIISRTEGLKRLRPGAITFCVMSRCCSNEPKRLISAAVGCAIPVDKKTYGYI
                     SEHHAFGFTEKKTGDYAEDLAAAMLASTLGIDFDVDESWDEKKELFRISGKIVGTRNT
                     TQSAVLKTADYTTVLAAAVFVL"
     gene            4563..4796
                     /locus_tag="PLR47_03160"
     CDS             4563..4796
                     /locus_tag="PLR47_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11967.1"
                     /translation="MNGRNQGGRGTGGGQGQGGGMGQGRGGGGRGRMGGPSAGGAVGT
                     CRCPQCGHTQPHERGVPCTQVKCPQCGTSMFRE"
     gene            4815..5177
                     /locus_tag="PLR47_03165"
     CDS             4815..5177
                     /locus_tag="PLR47_03165"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOG11968.1"
                     /translation="MPRGDATGPMGMGPMTGRAAGFCAGYSAPGYVNNGPGRAFGMGF
                     GRGAGFRRGFHAGGFGRRNRFYAGGAPVGAWAGGWNAPAAAPDPENEKQALKNQAEYL
                     QMQLDAVKKRLEELSAQS"
     gene            5255..5617
                     /locus_tag="PLR47_03170"
     CDS             5255..5617
                     /locus_tag="PLR47_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOG11969.1"
                     /translation="MKIAFTTAGNDVSAPLDSRFGRAPKFLIYDLDTGTFTLADNQQN
                     LNAAQGAGIQSAQNIARLGVSALVSGHCGPKAFRVLQAAGIKIYNSSAATVQEALDLF
                     RAGKLNEASSADVEGHWV"
     gene            5614..6105
                     /gene="tsaA"
                     /locus_tag="PLR47_03175"
     CDS             5614..6105
                     /gene="tsaA"
                     /locus_tag="PLR47_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOG11970.1"
                     /translation="MKNSRIEHRPIGVIHSEHTAAEKTPIQPVYAKGCKGRVEVYPEF
                     AEGLKDLGGFSHLYLLYHFHQCRKVQLTVKPFLQDVERGVFSTRAPCRPNPIGLSVVE
                     LIKMEGNILHVDGLDILDGTPLLDIKPYTEKFDLHAVKKNGWQDEVDEPTARIRGKRG
                     FRS"
     gene            6102..6989
                     /locus_tag="PLR47_03180"
     CDS             6102..6989
                     /locus_tag="PLR47_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOG11971.1"
                     /translation="MNMCLDCIPCFIRQAADTVRLSAPSEEDQNRLMHSVLQWLGNID
                     LRQAPPAAAQMIHRHLRSLLPTEDPYRASKDRHNQLAARLIPSIRRRIEASYDPLTMA
                     VRYAITGNIIDLGAKNNVGFGEIYAELQSAPMQPVFGDLEALKKASRQAKTILYLADN
                     AGEIFFDRLLIEQFTGAQVTLAVRGAPVINDATLLDAKAAGMEEIAEVMDNGSDAPGT
                     LLEDCRPEFRRRFEEADLVIAKGQGNFESLSDRARDIFFLFRAKCPVISLHSGFTAGT
                     YVASNWFKRSKTAPTGEAS"
     gene            6986..7594
                     /locus_tag="PLR47_03185"
     CDS             6986..7594
                     /locus_tag="PLR47_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11972.1"
                     /translation="MKPPFVAAELKAHAPFTAFGTLTGIVIMAAFLQFRVPGEVSAAL
                     FWIFHPLHVLLSALVTTAMYRRHSRSGIGRVFLIGYAGSIGIATLSDSLIPFAGEWLL
                     NLPNRGVHLGFIEKWWLVNPLAFAGIGLGFVVFHTQIPHAGHVLLSTWASLFHIMMSL
                     GSSIDVVTTILIAVFLFLAVWVPCCTSDIIFPLMWAKKEEAA"
     gene            7591..8451
                     /locus_tag="PLR47_03190"
     CDS             7591..8451
                     /locus_tag="PLR47_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG11973.1"
                     /translation="MRISVASGKGGTGKTTVSVNLARVFGSDVLLADCDVEEPNAHLF
                     LKTSGPAEKVVGIPVPVVNETLCDGCGECSKICQFSAIVTFGTTPLVFPEMCHGCGGC
                     TLVCPPRAITEKDRRIGVVETSRAGHITLVTGRLDVGVALAPPLIRAVKAELPAAADA
                     ILDAPPGTSCPVIAVVRDTDMVLLVTEPTPFGMHDLTLAVDMVRELDLPFGVIVNRMG
                     SGDDRVQHFCEKEGIPILLTIPDDRRIAENYSRGILMVDALPEYKEIFKALLQKIRAQ
                     IGCGSAPCGD"
     gene            8454..9407
                     /locus_tag="PLR47_03195"
     CDS             8454..9407
                     /locus_tag="PLR47_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG11974.1"
                     /translation="MHSGPFEYLYGPVPSRRLGRSLGIDLVPHKTCTYDCIYCQLGNT
                     THKTVERRDYVPVEGVLSELKEKLAAGVSCDYITLAGSGEPTLHASLGELIRKIKEMT
                     EIPVSVITNGSLLELPEVREALFRADLVVPSLDAGDPAMFEYVNRPHRDIVFERMIEG
                     LVTFGKRYPGRLWMEVLLVSGVTGLASEVKKIASWIEKFPVEKIQLNTVNRPSYEDFA
                     CAVDPKQMKTLAGLFAGAVEILENAPPTVGASETTSDTTDADICNLLARRPCTLEGVA
                     LGLNLHPHDAAKRIERLIGDKTVTAKRNGGKIYYTSAERTP"
     gene            9404..10291
                     /locus_tag="PLR47_03200"
     CDS             9404..10291
                     /locus_tag="PLR47_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG11975.1"
                     /translation="MKELVIISGKGGTGKTSVTASLAVLAGRPVVCDCDVDAADLHLV
                     LAPEIREEHEFRSGHQAVINRDHCADCGACADLCRFGAIREEEKEDSDTLFVVDAVSC
                     EGCGVCVHFCPEKAIDFPESLCGKWFVSKTRLGPMVHARLGVAAENSGKLVSTVRQEA
                     RRLAEKDHHNLVIVDGPPGLGCPVIASITGASQVLAVTEPTVSGEHDLERVLGLARHF
                     QIPAAVCVNKWDINDEMTGRIERKARDLGASIAGRIRYDPAVTAAQMQKKAVVEIEAP
                     CASDLRQLWLNLNLQEKKP"
     gene            10311..11051
                     /locus_tag="PLR47_03205"
     CDS             10311..11051
                     /locus_tag="PLR47_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOG11976.1"
                     /translation="MEDKKTCDSCGEESCSAKKQGKNENQEEYADRRRLQARLCRIQH
                     KIVVLSGKGGVGKSTVAVNLATALALHGLRVGLLDVDIHGPSVPTMLGLEGESLKGNP
                     GELIDAPPGTGDEPLSLCQLIQPLTGAVIVTTPQKIAAIDVRKSISFCRRVNVPVLGV
                     VENMSGFVCPKCGELTQILPEGGGRKIASDMKVPFLGAIPMDPALAQSGDSGRVFIYH
                     FSQTPTGKIMQDIIDQIQDMNSTKKLDS"
     gene            11064..11402
                     /locus_tag="PLR47_03210"
     CDS             11064..11402
                     /locus_tag="PLR47_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOG11977.1"
                     /translation="MKIAIPLAEGKLSMHFGHCASFALVDVDPQTKKIIKQEEIAAPP
                     HQPGLLPPWLAERGATMIIAGGMGQRAQQLFAQQGIQVVVGAPGETPERIITDYFAGT
                     LQIGENVCDH"
BASE COUNT         2671 a         3277 c         3359 g         2279 t
ORIGIN      
        1 ttccgacgcg ctcaggcgca aacgcatcga agcggtttcg gtcacggccc tggtcaacga
       61 cacggtggga acgctggcga caaaaagcta cgccgattct tcctgcgata tgggcgtcat
      121 cctcggaacg ggcacgaacg cctgctatcc ggaaaaaacc ggccgactgg caaaaggcct
      181 gtttccgctg cctagacagg aaatgatcct caatctggaa tggggcaatt tcgacaaact
      241 cgacaccaac gcctgcgatc aggcgctgga tcgcatgacc ctcaatcccg gctgccagcg
      301 catggaaaag atggtgtccg gaatgtatat cggcgaactg gtccgactga tcctgctgga
      361 tatgacggac cagggaatct tgcttcaaga aaagcaccgg cccgttctgg cccgcgaaca
      421 ctccgtcagc tctgagcaga tgtccttgtc cgccctgggg gaagatttct tcgcggccat
      481 cggcattccg gacgtctccg atcgggaccg ggacgccgcc gcgcacgtct gccggatcgt
      541 ctcgagacgg tcggcccgta ttgccggagc ggcgttggcc gccgtggtta cctggatgga
      601 cgcacatctt gaaaaaaagc ataccatcgc catcgacggc tccctttttg aaaaatatcc
      661 cggctatccg gaaaacgtgc ggtgcgtcct ggaagagttc ttcgcttgcc gtgccgaccg
      721 gatttcgctt tgtacgacgt cggacggctc gggaatcggc gcggccatca tcgccgccgt
      781 cgcgtccttc ggacaggagc aaaagcataa accggcccgt tagcgaatac gcaagaaccg
      841 ctgaaatcat tttccttgtt tccggtccgt tcctggttta tattcctgtc gaaagttctc
      901 cgaaaaataa aaagttgcaa agcacccggc aaccccggaa gccggcggca acccgacgaa
      961 aggagatcct catgaaaacg caatccggaa atttcaagtc gcaggacggg acgtccatct
     1021 actggcaggg atggacgccg gacgacgctt cccgccccaa agcggtggtg caggtcattc
     1081 acggctacgc cgaacacatc ggccggtatg ggaatgtggt gggtgaactt ctcccggcgg
     1141 gctacgcggt tttcggcacc gatcaccgcg ggcacggaaa aagtgaggga aagcgcggac
     1201 acgtcatgag ctttcaggag tttatcgatg atgaaatgca gttccggcgt gaagtcattc
     1261 ggacaaaatt ttccggcctt ccctgctttg tcctcggcca ttccatggga agcctcatcg
     1321 ccatgaacct cgtcgagcaa aacgccaatg aaattcaagg gcttgtgcta tcgggaacgg
     1381 gctcccggcc gggaacggac atcccgaaaa tcctgcttac agcaaccaaa atcctctcgg
     1441 gcctgctgcc gtcgattcac gtaaagtcgc cgctgccgcc ggagtttatt tcccgcgatc
     1501 ccgaggtcgt cagggcatat gtagaggacc ctctcgtcta caatgtgatc acgccgcggc
     1561 ttgcccatga gatgaaccgt tatgtcgtca tcggcgcgga gaatgccgga aaaattcaaa
     1621 tcccggtact gattcaactg ggctcacaag acaccgcctt ttccgggcag aaggaacttt
     1681 ttgaaatggt cggagcaaaa gacaagacat tcaaacgcta cgaaggcctc aagcatgaag
     1741 tgtacaacga gctcgcccac gaccggaccc gtgtgctcca agacctgcga tcctggctgg
     1801 acgctcatgt gtagggcagg tactgcccgg acggacggcc gccggacagt tattgaaggc
     1861 ggaaaagtca ccttgaacga aatctcttca cgccgcctgg ggaaaccccc cggtcccatg
     1921 agagagcatt gatcacaaaa gggtcccgta tgaaaattca gcggattcca aaacctttgc
     1981 ccttcgatcc tgaaagcgga caaaacatca ttctggattc catcgctgac ggtgttttta
     2041 ccgttgatca ggattggcgg atcacctctt tcaaccgggc cgcgcaggag atcacccggg
     2101 tttcccgcga ggaggcggtc ggccagatgt gcaaggatgt tctgaaagcg gacatctgcg
     2161 aaaaaaactg ctgtttgcgc atgaccatga aaaccgggaa gcccattatc aacaagaaag
     2221 tgaacatcat cgacgcggac ggcagaaagc ttccgatcag catttccacg gccctgcttc
     2281 gcgacgggaa gggaaaactt ctgggcgccg tggaaacctt ccgggacatc agcgtcgaag
     2341 aagatctgcg caaagctatt cagggaaaat attcttttga agatatcatt tccaagaacc
     2401 acaggatgct ccggctcttt gacatccttc ccgacatcgc cgccagctcc agcaccgtcc
     2461 tgattgaagg ggagagcggc acgggcaagg agctcgtcgc ccgcgccatt cataatctca
     2521 gccccagaaa aaggcagccc ttcgttgcgg tcaactgcag cgctctgccg gacacgcttt
     2581 tggagtccga gcttttcggc tacaaggccg gcgcgttcac ggacgcgaaa aaagacaagc
     2641 ccggacgctt ccacgcggcg gaaaacggca cgctgtttct cgacgagatc ggcgagatct
     2701 ctcccgccat gcaggtgaaa ctgctgcgag tcctgcagga aaaaacctat gagccgctgg
     2761 gaacaacccg cagcgtagag cacaacgtgc gcatcctcgc ggccaccaac aaaaacctgg
     2821 aagacctggt ccgccaaaac cgcttccgcg aggacctgtt ctaccggatc aatgtcttta
     2881 aaattcatct gccgccgctg cgcgaacgca tggaagacat tcctctactc attgaccatt
     2941 ttatcgaccg tttcaatatc ctgcaaaaaa agaatatcga agaaatcagc gacgatgcga
     3001 tggcgttgct gatggcgtac cgctatcccg gcaacatccg ggaacttgcc aacctgatcg
     3061 aacgcgcctt catcctctgc aaagagggcc ggattgaaaa gaagcattta cccgactttc
     3121 tgtttgcggc aaacacttcc ccggaaggaa aaaccgccgc gtcttttcgg gacctggaag
     3181 ccgactatct catcgacgct ctccgaaaga acaacggaaa ccgtccccaa accgcccggc
     3241 aactgggaat ccatcgaagc accctgtatc gtaagatcaa agcgctcaaa ctttctccga
     3301 acgattgacg attgcttgtc gcagatatgc atcattttgc gacaatgtcg cataaatgca
     3361 caaacggcca tcagggccat tcgccgcgag ctcatccaac cgcttgttat tgtttcattt
     3421 gttatttttc ctttttggca tcggttttgc gttggataaa agccgggtaa agttaggctg
     3481 ccgtttagaa aaggagagcg catgaagttg gccctgtcgg tatttaaaga ttgcatctca
     3541 accgtttttg actcctcgga tcaactgttg attctggaga cggaaggcgt caacggaacc
     3601 aaacggacga cggtgcggat tgacgcgacg gatccggccg ggcgcgcggc aagacttcgg
     3661 gaccagggcg ttgcggtttt gatctgcggc gcgatctccc gacccatgca gatggccatc
     3721 gcctcgctgg gcatcgcggt ttatccgttt attcgcggca cggtggaaga agttctggcc
     3781 gcctatcaaa gcaaccggct ggggcaggct gttttttccc tgccgggctg ccacggccgc
     3841 ggaaacggag gtaacggcag aggacgaggc atgcgctgcc gctggagaca aaccggcggc
     3901 catgaacgat aaagcacaac tcaaaaggag attaaaaaat tgaattcatc catcccgaaa
     3961 gaactgttct ttaccaaagg cgtcggcacg caccgcgaac agcttcactc gtttgaactg
     4021 gcgcttcgca atgcgggcat cgaaaaatgc aatctggtgc aggtctccag cattatgccg
     4081 cccggctgca aaattatttc ccggacggag ggcctgaaaa ggctgcgacc cggtgcgatc
     4141 acgttttgcg tgatgagccg ctgctgctcg aacgagccca aaaggctgat ttcagcggcg
     4201 gtcggctgcg cgattcccgt ggacaagaaa acctacggct acatcagcga gcatcacgcc
     4261 ttcggcttta cggaaaagaa aacgggggac tacgccgaag acctggccgc ggcgatgctt
     4321 gcctccacgc tgggcatcga ctttgacgtc gatgaaagct gggacgaaaa gaaagagtta
     4381 ttcagaatca gcgggaaaat cgtgggaacc cggaacacaa cacagtccgc cgtcctgaaa
     4441 accgccgact acacgacggt gctggccgcg gcggttttcg tgctctgacc gtacgatcat
     4501 ccggatcgag ggccatgaat aaaaacacaa caaaccggta aaaaccggaa ggaggaaaac
     4561 ccatgaacgg aagaaatcag ggaggcagag gaacgggcgg aggccagggc caaggaggcg
     4621 gcatgggcca gggacgcgga ggcggcggac gaggccgtat gggcggtccg tcagccggtg
     4681 gagcggtcgg aacctgccgt tgcccccaat gcggccacac acagccgcac gagcgaggcg
     4741 tgccctgtac gcaggtcaaa tgccctcagt gcggcacgtc catgtttaga gaataagaaa
     4801 ggaggaaaag tattatgcct agaggagatg caacaggccc gatgggtatg ggcccaatga
     4861 cgggaagagc ggctggattt tgcgcgggct attccgcgcc gggttatgta aacaacggtc
     4921 ccggaagagc gttcggcatg ggatttggaa ggggtgcggg ctttcgacgg ggctttcacg
     4981 caggcggctt tggccggcgc aaccgttttt acgcgggcgg cgcgccggta ggagcctggg
     5041 ccggcgggtg gaacgcgccg gcggctgcgc cggatcctga aaatgaaaaa caggcgctca
     5101 aaaaccaggc ggagtatctt cagatgcaac tggacgcggt caaaaagcgc cttgaagaac
     5161 tttcagccca aagttagtat ccatcatccg gatgggcgcc gcccccgggc cgcccgtccg
     5221 gatgatgaaa gatgcaaaaa agaggaagga aaacatgaaa atcgcattca caaccgccgg
     5281 aaatgacgta tcggcgccgc ttgacagccg cttcggccgc gcgccgaaat ttttgattta
     5341 cgaccttgac accggcacat tcactctggc ggacaaccag cagaatctga acgcggcgca
     5401 gggcgcgggc attcagtcgg cgcagaacat cgcacgcctg ggcgtgtcgg cgctggtcag
     5461 cggacactgc ggccctaaag cctttcgcgt tcttcaggcg gcgggcatca aaatctacaa
     5521 ctcctccgcc gcgaccgtac aagaggcgct ggatctgttc agggccggaa agctcaatga
     5581 agcatcctcc gccgacgtgg aaggtcactg ggtatgaaaa attcgcggat agagcaccgg
     5641 ccgatcggcg tcatccacag cgaacatacg gccgcggaga aaacccccat tcagcccgtg
     5701 tatgccaaag gctgcaaggg acgcgtggaa gtgtatcccg aatttgccga aggcttaaaa
     5761 gatctgggag ggttttcgca tctttatctg ctgtatcatt ttcaccaatg ccgcaaggtt
     5821 cagttaaccg ttaagccttt cctgcaggat gtggagcgag gcgtgttttc aacccgcgcg
     5881 ccctgccgcc ccaatcccat cggcttgagc gttgtcgagc ttatcaaaat ggaaggcaac
     5941 attctgcatg tcgatggact ggatatcctt gacggtacgc cgcttttgga catcaagccc
     6001 tacacggaaa agttcgacct gcacgccgtg aagaaaaacg gctggcagga cgaagtggac
     6061 gaacccacag cccgcattcg gggaaagaga ggttttcgat catgaacatg tgtctggatt
     6121 gcattccctg cttcattcgc caggccgctg acacagtccg cctgtcggct ccgtccgagg
     6181 aagatcagaa ccgtttgatg cacagcgtcc tgcagtggct cggaaacatc gacctgcggc
     6241 aggcgccgcc ggccgccgcg caaatgattc acagacacct gcgcagcctt ttgcccacgg
     6301 aggatcccta ccgggcttcc aaggaccgcc acaatcaact ggcggccagg ctgatcccct
     6361 ccatccggcg gcgcatcgaa gcctcttatg atcccctgac catggcggtg cgatatgcca
     6421 tcacgggcaa catcatcgat ctgggcgcca aaaacaacgt gggcttcgga gagatttacg
     6481 cggagctgca aagcgcgccc atgcagcctg ttttcggcga tttggaggcg ttgaagaaag
     6541 cgtcccggca ggcaaaaacc attctttatc tcgccgacaa cgcgggagaa atcttttttg
     6601 accgcctgct gatcgagcaa ttcaccggcg ctcaggtcac gctggccgtg cgcggcgctc
     6661 cggtcattaa tgacgcaacc cttctggacg caaaagcggc gggcatggaa gaaatcgcgg
     6721 aagtcatgga caacggttcg gacgcgccgg gaaccctgct ggaagactgc cgtccggaat
     6781 ttcgccgccg ctttgaagag gcggacctcg tcatcgccaa aggccaggga aattttgaaa
     6841 gcctgtcaga cagggcccgc gatattttct tcctgtttcg ggccaagtgt ccggtgatca
     6901 gccttcattc cggcttcact gcgggaacct atgtggcatc caactggttt aagagaagca
     6961 aaaccgcgcc gaccggggag gcttcatgaa accccctttt gtcgcggctg aactaaaggc
     7021 gcacgcgcct tttacggcct tcggaaccct gacgggcatc gtcatcatgg ccgccttcct
     7081 tcagttccgg gtcccgggtg aagtatcggc cgcgctcttc tggatttttc atccgctgca
     7141 cgtgctgttg agcgcgctcg tgaccaccgc catgtaccgg cgtcattccc ggagcggtat
     7201 cgggcgcgtg ttcctgatcg gttatgccgg ttccatcggc atcgccaccc tgagcgactc
     7261 gctcattccc ttcgccggcg aatggctgtt gaacctgccg aaccgcggcg tgcatttggg
     7321 ttttatcgaa aaatggtggc tggtcaatcc cttggcgttt gcgggcatcg gactgggctt
     7381 cgttgttttc cacacccaga ttccgcatgc cgggcacgtg ctgctcagca cctgggcgtc
     7441 cctttttcac atcatgatgt cgctgggctc atccatcgac gtcgtcacca ccattctcat
     7501 cgccgtcttt ttgtttttgg ccgtctgggt tccctgctgc accagtgata ttattttccc
     7561 gctgatgtgg gcaaagaagg aggaagcggc atgagaatat ccgttgcctc cggaaaaggc
     7621 ggaacgggga aaacgaccgt cagcgtcaat ctggcacgcg tatttggaag cgatgtgctg
     7681 ctggcggact gtgacgtgga agagcccaat gcgcatctgt ttttaaaaac ctccggaccg
     7741 gcggaaaaag tggtcggcat tcccgttccc gttgtgaacg agacgctctg cgacggctgc
     7801 ggcgaatgct cgaaaatctg ccagttctcc gcgatcgtga ccttcggtac aacccccctt
     7861 gtttttccgg aaatgtgcca cggctgcggc ggctgcacgc tggtatgccc gcccagagcg
     7921 attaccgaaa aggaccgtcg catcggcgtc gtcgaaacct cccgcgcggg ccatatcaca
     7981 ctggtcacgg gcagactcga cgtgggcgtg gcgctggccc cgccgctcat ccgcgccgtg
     8041 aaagccgaac ttcccgccgc ggccgacgcc atcctggacg ctccgcccgg cacgtcatgc
     8101 ccggtgatcg ccgtcgtccg ggatacggac atggtgcttc tggttaccga acccacgcct
     8161 ttcggcatgc atgacctgac actggccgtg gatatggtga gggagcttga ccttcccttc
     8221 ggcgtcatcg tcaaccgaat gggcagcgga gacgatcgcg tgcagcattt ttgcgaaaaa
     8281 gaagggattc ccatcctgct tacgattccc gacgaccggc ggatcgcgga aaactattcg
     8341 cggggcatcc tgatggtcga cgccctgccg gagtacaaag aaattttcaa ggcgctgctt
     8401 caaaaaatcc gcgcgcagat cggctgcgga tcagcccctt gcggagatta aaaatgcatt
     8461 ccggtccatt tgagtatctg tatggccccg tcccgtcccg aagactcgga cgttcgctgg
     8521 ggatagacct ggtgccgcat aaaacctgca cctatgactg catctactgc cagctgggca
     8581 acacgacgca taaaaccgtt gaaaggcggg attatgttcc cgtggagggc gttctttccg
     8641 agctgaagga aaagctggcc gccggtgttt cctgcgacta tatcacgctc gccggttcgg
     8701 gtgagccgac gctgcacgcg tctctgggcg aactgatccg caagatcaag gaaatgacgg
     8761 agattcccgt ttcggtgatc accaacggtt ctttgcttga gctgccggag gtgcgtgaag
     8821 cgcttttccg ggcggatctc gtcgtccctt cactcgacgc gggagacccg gccatgtttg
     8881 aatatgtcaa ccgcccgcac cgggatattg tatttgaacg gatgatcgaa ggccttgtaa
     8941 cgttcggaaa acggtacccg ggtcgtctgt ggatggaagt gttgctggtt tccggcgtca
     9001 cggggctggc ttcggaagtc aaaaaaatcg cgtcatggat tgaaaaattc cccgtggaaa
     9061 aaattcagct caataccgtc aaccggccgt cctacgagga tttcgcctgc gccgtggacc
     9121 cgaagcaaat gaaaaccctg gccggtcttt ttgccggcgc ggttgaaatt ctagagaacg
     9181 cgccgccgac cgtcggcgca tcggaaacaa catcggacac aacggatgcg gatatttgca
     9241 atttactggc ccggcggccc tgtacgctgg aaggcgttgc tctgggcctc aatctccatc
     9301 cgcatgacgc ggccaagcgc atcgaacggc tgatcggcga caaaaccgtc acggcaaaac
     9361 gaaacggagg aaagatttat tatacttcgg cggagcgaac accatgaaag aactggtcat
     9421 catcagcggc aagggaggga cgggaaaaac cagcgtcacg gcgtctctgg ccgttctggc
     9481 cggacgtccg gtcgtgtgcg actgcgacgt ggacgcggcg gacctgcacc tggtcctggc
     9541 gcctgaaatc cgggaggagc atgaattccg cagcggccat caggccgtga tcaaccggga
     9601 tcactgtgcg gactgcggcg cctgcgcgga cctgtgccgg ttcggggcga tccgggaaga
     9661 agaaaaggag gacagcgata cgctttttgt cgtcgatgcc gtatcctgcg aaggctgcgg
     9721 cgtgtgcgtt catttttgcc ccgaaaaagc cattgacttt cccgagagtc tctgtggtaa
     9781 atggttcgta tccaaaacgc gcctcgggcc catggtgcac gcgcgtctcg gcgtcgccgc
     9841 cgaaaattcc ggaaagctag tctccacggt tcgtcaggag gctcgacggc tggctgaaaa
     9901 agatcaccat aacctggtga tcgtggacgg accgccgggg ctgggctgtc ccgtcatcgc
     9961 ttcgattacc ggtgcctcgc aggttttggc ggtaaccgaa cccacggtgt cgggtgaaca
    10021 tgaccttgaa agggtgctgg ggctggcccg gcattttcag attccggcgg ccgtctgcgt
    10081 caataagtgg gacatcaatg acgaaatgac gggccgcatc gagcgaaaag cccgtgatct
    10141 gggcgcaagc attgccggac gcattcggta tgacccggcc gtgaccgccg cgcagatgca
    10201 aaaaaaagcg gtggtcgaaa tagaagcgcc ctgcgcctcg gatctccggc aattgtggct
    10261 gaatttgaac cttcaggaaa aaaaaccgta acaggaggaa agtagagaaa gtggaagaca
    10321 aaaaaacatg tgattcgtgc ggcgaggaga gctgctcggc gaaaaaacag gggaaaaatg
    10381 aaaatcagga agagtatgcg gaccgacggc gcctgcaggc caggctgtgc cgcattcagc
    10441 ataaaattgt cgtgctctcc ggcaagggcg gcgtcggcaa aagcacggta gcggtcaatc
    10501 tggcgacagc gctggcgctc catggactgc gagtgggttt gttggacgtg gacatccacg
    10561 gcccgagcgt ccccaccatg ctgggactgg aaggtgaatc gctcaaaggc aatcccggcg
    10621 aacttatcga tgctcctccg ggcaccggcg acgaaccact gtccctgtgc cagttgattc
    10681 agccgcttac cggcgcggtg atcgtgacca caccgcagaa gatcgcggcc atcgacgtgc
    10741 gtaaatccat cagcttttgc cggcgggtca atgtgcccgt tctgggcgtt gtggaaaaca
    10801 tgagcggttt tgtctgcccc aagtgcggag agcttacgca aattttgccg gagggcggag
    10861 ggcgaaagat tgcttccgat atgaaggttc cgtttctggg cgcgattccc atggatccgg
    10921 ccctcgccca gtccggcgat tccggccggg ttttcatcta tcatttttcg caaacgccaa
    10981 ccggaaaaat catgcaggat attatcgatc agattcagga catgaactca acaaagaagc
    11041 tagattccta aggagaaaca accatgaaaa tcgccatacc gttagcggaa ggaaaactgt
    11101 cgatgcactt cggccattgc gcgagctttg cgctggtgga cgtggatccg cagaccaaaa
    11161 aaattatcaa acaggaagaa atcgccgctc cgcctcacca gcctgggctt ttgcccccgt
    11221 ggctggccga acgaggcgcg accatgatta ttgccggcgg catggggcag cgggcccagc
    11281 agcttttcgc ccagcagggc attcaggtgg tcgtgggcgc gccgggcgaa acaccggaaa
    11341 gaatcattac ggattatttc gcgggcacgc tgcagatcgg agaaaacgtc tgcgaccact
    11401 gaacgaaacc tgcaacgttg gatcggaagc aacgacattc catcggggcg ccggaaaact
    11461 atccggtttg cctcaaataa aaggaggggc aaagttatgg cggatgcagc attgaaaatt
    11521 gtggaaaaag tagaaattct gaccctgcag gacaattaca tcgagatgac ggccatggac
    11581 aacaat
//
LOCUS       DAOSIM010000041        11544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_5565_length_11544_cov_7.216903, whole genome shotgun sequence.
ACCESSION   DAOSIM010000041 DAOSIM010000000
VERSION     DAOSIM010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 11544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11544
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5565_length_11544_cov_7.216903"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1848)
                     /gene="aspS"
                     /locus_tag="PLR47_03215"
     CDS             complement(76..1848)
                     /gene="aspS"
                     /locus_tag="PLR47_03215"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOG11978.1"
                     /translation="MSRFIVKEKRTHYCGSVTVADEGNEIVLMGWVHRRRDHGGVIFV
                     DLRDREGIVQVVFQPDAGEAVHGEAHKIRSEYVLAVKGTVRRRPEGMDNPALPTGQVE
                     VVISELEILNESKTPPFSFDDEDISENVRLKYRYLDLRRPAIQKNLFLRSRLAIATRQ
                     YFNDHGFIEVETPFLTKSTPEGARDYLVPSRISKGMFYALPQSPQIFKQLLMVSGFDR
                     YFQIVKCFRDEDLRADRQPEFTQIDVEMSFISEEDILQMMEGLMAAIFKTCMHIDLPL
                     PFPRLTFAEAVSRYGKDNPDVRFGMEITDLTSIVKDSGFKLFAEVVASGGVVKAVKAE
                     QASSLSRKDLDSLSDFVAVYGARGLAWARINAADWTSPIFKFLKPEESAAIARAMNAR
                     EGDILFFVADSPKTACDALGNLRLHLGKKLNRINPEALAFTWVTEFPLMEYSESEKRF
                     VSTHHPFTSPFLEDIPLMSTDPGKVRARAYDLVLNGSEIGGGSIRIHRKNVQAQVFSA
                     LGLSDEEAKLKFGFLLDALEYGAPPHGGLAFGLDRLVMIMTGAESIRDVIAFPKTQKA
                     ADLMSDAPSRVSIEQLMELSLKIV"
     gene            complement(1845..3119)
                     /gene="hisS"
                     /locus_tag="PLR47_03220"
     CDS             complement(1845..3119)
                     /gene="hisS"
                     /locus_tag="PLR47_03220"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOG11979.1"
                     /translation="MEKITAVKGIKDVLPGDSPVWHRLELAARRVFECFGFCEIRVPI
                     MEKTSLFQRSIGEATDIVEKEMYTFRDRDDELLTLRPEATASVIRAYIEHNLSASDPV
                     TRLYTMGPMFRRERPQKGRFRQFHQIDAELFGDDSPAADAEVIFMLMHFLQTLGIGKL
                     SLEINSLGCKACRPVFSGAVVDYLKGRESSLCPDCQRRIHTNPLRVFDCKVESCGEVI
                     DGAPRILDFLCADCSEHFSRVKSYLSDLNLPYEINPRMVRGLDYYTRTAFEVKSGGLG
                     AQNSLAGGGRYDGLVQFLGGPDIAGIGFGIGMERLIACLPPDDRNPYAAEIFIAALGE
                     RAQKIAFTLTQELRRRGICAAMDYSGKSLKSQMKRADKLHSAYTLIFGDREIEENCAE
                     LRDMNTKNQQRLPLDQLLEAVTDILKREVKVL"
     gene            complement(3157..5679)
                     /locus_tag="PLR47_03225"
     CDS             complement(3157..5679)
                     /locus_tag="PLR47_03225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOG11980.1"
                     /translation="MRRPLWYPLAALMAGIWIGEGISPAPAVLPVCMLPVLTLLLVSV
                     SRRWNRAAFLLLLALMLTAGMLNIQRQPFLAGQDRHILHSAGQGKTTLEGIVLSTEQA
                     LPERNTLIVDCRRVLRDSTAVPATGKIRLAIPPDLSFQYGDFIRVHTKIREIQSFHNP
                     GSFDYERVQNRRGIYVAGYVSDRAGIVLIRPGAAGGLRLKLERFRLRLKRLIEARAPS
                     PQREILQALTIGNQKSIPQNVRDDFAKTGTSHILSISGLHVGIVAASAFFLILIALKS
                     SEYLMLKFNIIKAATGAAMVPVLIYALVAGMGTPVLRSALMTLAFLAALLIGRSKDLY
                     NILAGAALLILIVLPEALFEISFQLSFSAVFAILYAVDKLGNRRAPLPSSTPRWISAG
                     IGRLYLFLLVSVAATLGTLPIIVFYFNRVSAVTLIANLVAVPLLGILALIPAMLFILT
                     SLISEALAGFFISVSSFFTGIAVRIIEELASLPWSSFSFVKPNVMEIALFYLFLYFLV
                     QALSSGSGKDHSTRRPILMRTGLMICVALILADAAFLVLKDRYSTHLKITAIDVGQGS
                     STLLQLPRGVNMLIDGGGFHDSSFDMGRAVIAPFLYAKRIRKVDIAVLTHPHPDHLQG
                     LIYILNNFDVREVWCTGLTADDDLYRLWKNTISGRGVKVRNLSAQSPPVNLSGVNFRF
                     LWPPLPPDPVGRALSYEDTNDASLVMKITYGTRRFLITGDISASVEARLIASGEDLKS
                     DVLFVPHHGSATSSSPRFVRAVSCRWAVVSAGKNNVFRHPHTAVLERYRSAGAGIFRM
                     DRDGAVSIDSDGRALSITPWIKRPEPEPAGRRALNISLPMPG"
     gene            5787..7445
                     /locus_tag="PLR47_03230"
     CDS             5787..7445
                     /locus_tag="PLR47_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG11981.1"
                     /translation="MRQPMQYDERTWRRLKLLIVSRVVILSAFLLVTLFLGTVKSIIF
                     ISRDMLHAIYAVIVAIYILSVVYTLLLRKKKYFQINIYFQLAADIVLVSFLVYLTGSI
                     GSNYSLLYTLVIIYAAIFLGRRGALIFASVCSIAYGLVLDLEYFGVIPTFYATGRDYT
                     LEAGDVFIRIAVHILSFYILAFLASFVVEQEKKARSLLAEKESAFDQLDLLFRSIIES
                     VDTGILTTTLQGRIKTFNRSAEKITGHPLRSVENRQIEDVFPAFAPLFSNGDGPQMKP
                     KGEIKINSQKGVPIYLGYALSPLKDKNSGQIGHILIFQDMTEIRRMEENLEKSRRLAL
                     IGEMAAGLAHEMRNPLASITGSIQLMAQTPNLDETNKRLMQIILRGKDRLESFVRDFL
                     LLARPVPEAKQFVRVNDVAEEVVDSMKLSADWREGIQLEKRISGDPQALANEEQVRQI
                     LQNMLTNALQSMEGKGRIDLEIRTVRLEDKRDYAEIKVTDDGCGIEEKDMKKVLEPFF
                     TNKERGTGLGLAIVSRIVDGYGGRITIRSRVNEGTAITVWLPCR"
     gene            7464..8828
                     /locus_tag="PLR47_03235"
     CDS             7464..8828
                     /locus_tag="PLR47_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG11982.1"
                     /translation="MAKILVLDDDQGMREFMEIMLTKEGYDVTCAGKPEKAISLCRKT
                     AFDLVITDLKMPKIDGIEFLKTIKEQRPETVVILITAYASGETAVNAMKEGAYDYVEK
                     SGNIDELKKIVRQALLQQGSPGEAQTPKKNPPAETSFCGMIGASREMAKVFSTIRKVA
                     DTPANILILGESGTGKELVARAIHENSSRAPMPFMAINSGGIPENLLESELFGYMKGS
                     FTGAYGDRAGLFELARGGTVFLDEIGELPPVLQVKLLRVVQQKTFRRIGGDKDITVDV
                     RIISATNQNLKEKVARGSFREDLYFRLNVIPIHMPPLRERKEDIPLLTRHFIEKYARD
                     FGKEVRTISSYAMELLMEYPFPGNIRELENIIERGVAMETSNIILPENLVLTTDADAP
                     GVSIDLSIPDGGIDLNEEMNRIEKRAIEKALQKTRGIKTKAADLLGVTSDSLKYRIEK
                     LGIE"
     gene            complement(8847..10640)
                     /locus_tag="PLR47_03240"
     CDS             complement(8847..10640)
                     /locus_tag="PLR47_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOG11983.1"
                     /translation="MMFLLKQVVHRLRSIFLENIARWLVFGLLVGIVSGLGASAFYYL
                     LQAGRHFCFNLLAGYALPAPAGEQLFEAAGTLQFHRLVFFLLPALGGLISGLLVYHFA
                     PEAEGHGTDAMIDAFHNKKGRIRTRVPLIKGLASIITLSSGGSAGREGPIAQIGAGIG
                     SAIARLLRLSDRERRILLLAGCGGGLSAIFRAPLGGALTAIEILYKEDMETEALVPTV
                     VSSITAYIIFTYFFGNAPIFFFPEYTFSNPRELIFYILLGLICVPAGIFFIKVFYAVR
                     ERIFQPLKIPLYVKPALGGLAVGCIGLFFPQVYGDGWGWIQLAILGKLGIGIMMAIAL
                     AKIIATSFTISSGGSGGVFGPTLFIGGMIGGAVGYSAHYFYPNIVAQPEAFVVVGMAS
                     FFAGVASAPIGTLLMCSEMTRGYGLIAPLMLVSIIAILFTRQHSIYARQVQSKRESPA
                     HTADFTVNLFAETRVRDFYKPEKVTPVRKTTSYGQLKKIFSASRIECFPVYDENDALI
                     GSVNWDHARTIVFEEGLEDLIIAEDMMSPVRTVTPEDNFYDAFMKFMETNAEELLVVS
                     PDDGNIVLGVLRHDDLIAAYSAELNRRKNSG"
     gene            complement(10825..>11544)
                     /locus_tag="PLR47_03245"
     CDS             complement(10825..>11544)
                     /locus_tag="PLR47_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG11984.1"
                     /translation="TYSRLTVGLSAAAYMTRAVREAEKYVSFRDAFGLRLNQFPLVAV
                     QLDRIRKTTRRTTAGAFKLYRDFLKLEGGLKGSRGDDEPLPARRHRFNIRELIMLQKI
                     AASWDCTDVIREAMSLFGGHGVMEDFSCLPRLYRDSAINELWEGPRNVLLTQIHRDLQ
                     KAAGWYKPEEFVAEILQGLDKAQVQEMAEEIKALVAHPHLFGMDEATIDVCRRWDAFC
                     HRFSHAYQDLAVKEMDSPLKG"
BASE COUNT         2694 a         3183 c         3392 g         2275 t
ORIGIN      
        1 gccgaaaacg gtcgtcgagg ccgtggcctt tgccaaaaag gtcgccctgg aaatcaaccg
       61 gtactgcctg gagcattaaa caatcttcag cgacaattcc atcagctgtt caatgctcac
      121 ccgggaaggg gcgtcggaca tcaggtccgc ggccttttgc gttttcggaa aggcgatgac
      181 gtcgcggatc gattccgccc ccgtcatgat catcacaagc cggtccagac cgaacgccag
      241 tcctccgtgc ggcggcgccc cgtactcgag ggcgtcgagc agaaatccga atttcaactt
      301 cgcttcttcg tcgctgaggc ccagcgcgct gaacacctgc gcctgcacgt tcttgcggtg
      361 gatgcggatg ctgcccccgc cgatctcgga gccgttgagc accaggtcat aggcccgggc
      421 ccgcaccttt ccgggatcgg tgctcatgag cgggatgtct tccagaaacg gcgaggtgaa
      481 gggatgatgc gtggagacaa acctcttttc cgactccgaa tattccatta gcggaaactc
      541 cgtgacccag gtaaaggcca gcgcttccgg gtttatccgg ttcagctttt tgcccaggtg
      601 cagacgcaga ttgcccaggg cgtcgcaggc tgttttgggc gagtccgcga caaaaaagag
      661 gatgtcgcct tctctggcat tcatagcccg ggcgatagcg gccgactctt caggctttaa
      721 aaacttgaag atgggagagg tccagtcggc ggcattgatc ctggcccagg ccagtcccct
      781 ggcgccgtaa accgccacga aatccgacag agagtcgagg tctttccggg aaagagaaga
      841 agcctgctcg gctttgacgg ctttgacgac gcctccggag gcgacgacct cggcaaacag
      901 cttgaagccc gaatctttga cgatggacgt gaggtccgtg atctccatgc cgaagcgcac
      961 atcggggttg tctttgccgt aacggctgac ggcctcggca aaggtcagcc gtggaaaagg
     1021 aagaggcagg tcgatgtgca tgcaggtttt gaaaatcgcc gccatcagcc cttccatcat
     1081 ttgcagaatg tcttcttccg aaataaagga catctccaca tcgatctgcg tgaattccgg
     1141 ctggcggtcg gcgcgcaaat cctcatcgcg gaagcatttg acaatctgga aataacggtc
     1201 gaacccggaa accatcagca gttgcttgaa aatctgcgga gactggggga gcgcgtaaaa
     1261 catgcctttg gagatgcggc tgggcacaag gtagtccctc gcgccttccg gcgtgctctt
     1321 ggtcagaaag ggcgtttcca cttcgatgaa tccgtggtca ttgaaatact gccgcgtggc
     1381 aatggccagt ctgcttcgca aaaaaaggtt cttttgaatg gccgggcggc gcaaatccag
     1441 atagcggtat ttcagccgga cattttctga gatgtcttca tcgtcaaacg agaagggcgg
     1501 cgttttggat tcattcagga tttccagttc ggaaatgacg acttccacct gcccggtggg
     1561 cagcgccggg ttatccatgc cctccggcct tctgcgcact gtgcctttca cggccagaac
     1621 gtactcgctg cgtatcttgt gcgcttctcc gtggacggct tctcccgcgt cgggctgaaa
     1681 aacgacctga acgatgcctt cgcggtcgcg caaatcgaca aaaatgacgc cgccgtgatc
     1741 gcgccgccgg tgcacccaac ccattaaaac aatttcgttg ccttcatcgg cgacggttac
     1801 gcttccgcag taatgggttc ttttttcttt cacaataaaa cgactcacaa aacctttacc
     1861 tctcttttta aaatatccgt aaccgcttcc agaagctggt ccagcggcag acgttgctga
     1921 tttttcgtgt tcatgtcgcg cagttcggcg cagttttcct cgatttccct gtccccgaag
     1981 atcagcgtgt aggcgctgtg gagcttgtcc gcgcgcttca tctgactttt caggcttttg
     2041 ccggaataat ccatggccgc gcaaattccc cttcggcgca gttcctgcgt cagagtaaaa
     2101 gcgattttct gcgcgcgctc ccccagggcg gcaataaaga tttccgccgc ataaggattc
     2161 cggtcgtcgg gcggaaggca ggcgatcagc ctttccattc cgatgccgaa cccgatgccg
     2221 gcgatgtcgg ggccccccag aaattggacc aggccgtcat agcggccccc gcccgcgagc
     2281 gaattctgcg cgccaagccc gccggatttc acctcaaaag ccgttcgggt atagtaatcc
     2341 agaccgcgca ccattcgcgg gttgatttca tagggcagat tcaggtcaga cagatagctt
     2401 ttgacccggg aaaaatgctc cgaacagtcc gcgcacagga agtccaaaat gcggggagcg
     2461 ccgtcaatca cctcgccgca ggattccacc ttgcagtcaa agacgcgcag cgggttggtg
     2521 tgaatgcgtc tctggcagtc ggggcacagg gaactttccc ggcctttcag ataatcgacc
     2581 accgccccgg aaaagaccgg acggcaggct ttgcagccca gggaattgat ttccagagac
     2641 agttttccga tgcccagggt ttgcaggaag tgcatcagca tgaagatgac ttccgcgtcg
     2701 gcggcggggc tgtcgtcccc gaagagctcc gcgtcaatct ggtgaaactg gcggaatcgc
     2761 cccttctgcg gacgctcgcg gcggaacatg gggcccatgg tataaagccg ggtgacggga
     2821 tcggaggcag agagattatg ctcgatgtag gcccgaatga cggatgccgt ggcttccggg
     2881 cgcagcgtca aaagttcgtc gtcgcggtcg cgaaacgtgt acatttcctt ttcgacaatg
     2941 tcggttgcct ccccgatgct tcgctgaaac agcgacgttt tttccataat cggaacgcgg
     3001 atttcgcaaa agccgaagca ttcgaaaacc cggcgggccg caagctccaa ccggtgccag
     3061 acgggtgaat cgccggggag gacgtctttt atgcctttaa cggccgtaat tttttccata
     3121 gttatattcc aaaataaatc agatgcttct agcatatcaa ccgggcattg gcaatgaaat
     3181 attcagggcc cggcggccgg cgggttcagg ctccggcctt ttgatccacg gggtgatgga
     3241 aagggcccgc ccgtcggaat cgatggacac ggcgccgtcc cggtccatgc ggaagatccc
     3301 ggcgccggcg gaccggtagc gctccagaac ggcggtgtgg gggtggcgaa agacgttgtt
     3361 tttccccgcg ctgacgacgg cccagcggca ggacacggcg cggacaaagc gaggcgaact
     3421 ggatgtggcg gaaccgtgat gcggcacgaa aaggacgtcg cttttgaggt cttccccgga
     3481 tgcgatcagg cgcgcctcaa cggacgcgga gatgtcgccc gtgatgagaa aacgccgggt
     3541 gccgtaggtg attttcatga ccagggatgc gtcattggta tcctcatagg acagggctcg
     3601 gccgaccggg tccggaggaa gcgggggcca caggaaccgg aagtttacgc cggaaagatt
     3661 cacgggcggc gattgcgccg acaggtttct gacctttacg ccgcgtccgg agatggtgtt
     3721 tttccacagc cggtaaagat catcgtcggc tgtgaggccg gtgcaccaca cttcccggac
     3781 gtcaaagttg ttgagaatat aaatcagacc ctgaaggtga tcggggtgag gatgcgtcag
     3841 cacggcgata tcgacttttc gtatcctttt ggcgtaaagg aaaggggcga tcacggcccg
     3901 gcccatgtcg aacgagctgt cgtgaaagcc tccgccgtca atgagcatgt tgacgccccg
     3961 gggaagctga agaagcgtcg atgagccctg gccgacatcg atcgccgtga ttttcagatg
     4021 ggtggaataa cggtccttca ggacaaggaa agcggcatcg gcaagaatca gtgcaacgca
     4081 gatcataagg ccggttcgca tcaggatcgg ccggcgcgtc gaatggtcct tcccgctgcc
     4141 ggacgacagg gcctggacca ggaagtagag aaaaagataa aaaagcgcta tttccatgac
     4201 gttgggtttt acgaagctga aggaggacca gggcagcgac gccagttctt cgatgatcct
     4261 gacggcgatg ccggtgaaaa aggacgagac ggatatgaaa aaaccggcaa gcgcctccga
     4321 gatgagcgac gtcagaatga acagcatggc gggaatgagc gccaggatgc ccagcagggg
     4381 aaccgcaacc agattggcga tgagcgtcac ggccgaaacg cggttgaagt agaaaacgat
     4441 gatcggcagg gttcccagcg tggccgcaac ggagaccaga aggaagaggt acagccggcc
     4501 gatgcccgcc gaaatccagc ggggcgtcga tgaggggagg ggcgcccgcc ggttgcccag
     4561 cttgtcaacc gcgtaaagaa ttgcgaaaac cgcgctgaaa gaaagttgaa aagaaatctc
     4621 gaaaagcgct tcgggcagaa cgatcaggat gagcagcgcc gcgccggcaa ggatgttgta
     4681 gaggtccttg gaccgcccga tcagaagcgc ggccagaaag gccagcgtca tgagcgcgga
     4741 gcgaaggacg ggggttccca ttccggcgac cagcgcgtag atcaggaccg gaaccatggc
     4801 tgctcccgtc gccgccttga tgatgttgaa tttcagcatc agatattccg aggatttaag
     4861 ggcgataagg atcaggaaaa aagccgatgc cgcgacgatg ccgacatgca ggccggaaat
     4921 ggacagaata tgggaggttc cggttttcgc gaaatcgtcg cgcacattct gcggaatgga
     4981 tttttgattg ccgatcgtca gcgcctgcag aatttccctc tgcggggaag gggcgcgggc
     5041 ttcaatcagg cgcttcagac gcagacggaa gcgctccagc ttcagcctga ggccgccggc
     5101 ggcgcccggg cggatcagaa caatgcctgc ccggtcggag acatatccgg caacataaat
     5161 gccgcggcgg ttctggaccc gctcgtaatc gaagcttccg ggattgtgaa aactctgaat
     5221 ttccctgatt tttgtatgaa cacggatgaa atcaccgtat tgaaacgaaa ggtccggcgg
     5281 tatggccaga cgaattttcc ccgttgcggg tacggcggtg ctgtcgcgaa ggactctccg
     5341 gcaatccacg atcagcgtgt ttctctcggg aagcgcctgt tcggtggaaa gaacgatgcc
     5401 ttccagagtc gtttttccct gaccggccga atggagaata tgccggtcct gaccggcaag
     5461 aaagggctgc cgttggatat tcagcattcc ggccgtcagc atcagggcca gcagcagaag
     5521 aaaggctgct ctattccagc ggcgactgac gctcaccagc aggagcgtca ggaccggcag
     5581 catgcagacc ggcaaaacgg cgggggccgg agagatgccc tcgccgatcc agatgccggc
     5641 catcagggcg gccagcgggt accataaagg tctgcgcatg gggaacagga cctctcagcg
     5701 ggaagcgttc aattgattgg ctaaaaaacg caaaatatta taaaagcagc tcaaaatact
     5761 cacgcggaag aaccacggaa cagtttatgc ggcaaccgat gcaatatgac gagagaacct
     5821 ggcgaaggct caagctgctg atcgtaagca gggtggtcat cctctccgct tttctgctgg
     5881 tgaccctgtt tctgggcacc gtcaaatcga tcattttcat ctcccgggat atgctgcacg
     5941 ccatttacgc cgtcatcgtc gcgatataca tcctgtccgt cgtttataca ctgcttttaa
     6001 gaaaaaaaaa gtattttcag atcaacatct acttccagct tgccgcggat atcgtgctgg
     6061 tttcgtttct ggtgtacctg accggcagca tcggcagcaa ctactcgctt ctgtacacac
     6121 tggtcatcat ttacgccgcc atttttctgg ggcgccgggg cgccctgatc ttcgcgtcgg
     6181 tctgcagcat cgcctacggg ctggtgctgg atcttgaata tttcggcgtc atccccactt
     6241 tttacgcgac cggccgggat tacacgctgg aggcggggga tgttttcatt cggatcgccg
     6301 tccacattct gtcgttttac atcctcgcgt ttctggccag cttcgtagtc gagcaggaaa
     6361 aaaaggcccg gtccctgctt gcggaaaaag aatcggcttt tgaccagttg gaccttcttt
     6421 ttcgcagcat catcgaatcg gtggataccg gcattctgac gaccacgctg cagggccgga
     6481 tcaaaacatt caaccggtcc gccgaaaaaa taaccggcca tccgcttcga tccgtggaga
     6541 accggcagat cgaggatgta tttcccgcct ttgcccctct gttttccaac ggcgacggcc
     6601 cgcagatgaa gcccaagggt gaaataaaaa tcaacagcca aaagggtgtg ccgatctatc
     6661 tgggatacgc gctttccccc ctgaaagaca agaacagcgg gcagatcggc catattctga
     6721 tttttcagga catgacggag attcggcgca tggaggaaaa tctcgagaaa agcagaaggc
     6781 tcgcgctgat cggcgaaatg gccgccggcc tcgcccacga aatgagaaat cctctggcgt
     6841 cgattaccgg ctcgatccag ctgatggcgc agaccccgaa cctggacgag acgaacaaga
     6901 ggctgatgca gatcattctg aggggcaagg accgcctgga aagtttcgtg agggattttc
     6961 ttctgctggc ccgtcccgtg ccggaagcca agcagttcgt ccgggtgaat gatgttgccg
     7021 aagaagtggt ggatagcatg aaattgtccg ccgactggag ggaaggcatt cagctggaga
     7081 aaagaatttc cggagaccct caggcgctgg ccaacgaaga gcaggttcgt cagatcctgc
     7141 aaaacatgct gaccaacgcc ctgcagtcca tggaagggaa gggcaggatc gatcttgaaa
     7201 tccggaccgt gcgcctggaa gacaaaaggg actatgccga aatcaaggta acggacgacg
     7261 gatgcggcat tgaagaaaaa gacatgaaaa aggttttgga accgttcttc accaacaagg
     7321 agcggggcac ggggctgggt ctggcgattg tcagccgcat cgttgacggc tacggcggca
     7381 ggatcacgat acgaagcagg gtcaatgagg gaaccgcaat cacggtatgg ctgccctgcc
     7441 gataagatca aaggagcgca agcatggcga aaattctggt tttggatgat gatcagggga
     7501 tgagagagtt catggaaatt atgctgacca aggaaggcta tgacgtcacc tgcgccggca
     7561 agcccgaaaa ggcgatcagc ctgtgccgca aaaccgcctt tgacctggtg atcaccgacc
     7621 tgaagatgcc gaagatcgac ggcatcgaat ttctgaagac catcaaggag cagcggccgg
     7681 aaaccgttgt gatcctcatc accgcctacg cttccggcga gacggccgtc aatgccatga
     7741 aggaaggcgc gtatgactac gtggaaaaaa gcggcaacat cgacgagctg aagaagatcg
     7801 tccggcaggc cctgttgcag cagggatcgc cgggcgaggc ccagacgccg aaaaaaaatc
     7861 cgccggcgga aacctcgttt tgcggcatga tcggggccag ccgggagatg gccaaggttt
     7921 tttcgaccat tcgcaaggtg gccgatacgc ccgccaatat cctgattctg ggggaaagcg
     7981 ggacgggcaa ggaactggtc gcccgcgcca ttcacgaaaa ctcctcccgc gccccgatgc
     8041 cgttcatggc catcaacagc ggcgggatac cggaaaacct gctggaaagc gagctgttcg
     8101 gctacatgaa ggggtccttc accggcgctt acggcgaccg ggcgggcctg tttgaactgg
     8161 cccggggcgg aaccgttttt ctcgacgaga tcggggagct tccgcccgtt ctgcaggtga
     8221 agcttctgag ggtcgtgcag cagaagacct ttcgccgcat cgggggggac aaggacatca
     8281 cggtggacgt gcgcatcatc tccgccacca atcagaatct gaaggagaag gttgcccgtg
     8341 gatctttccg cgaagatttg tattttcgcc tcaacgtcat tccgattcac atgccgccgc
     8401 ttcgcgaaag gaaggaggac attccgcttc tgacccggca tttcattgaa aaatacgcgc
     8461 gcgatttcgg caaggaagtg cggacgattt cctcttacgc catggagctg ctgatggaat
     8521 atccctttcc cggaaatatc cgcgaactgg aaaacatcat cgaacggggc gtggcgatgg
     8581 agacctccaa tatcatcctg cccgaaaatc tggtgctgac gacggacgcg gacgcgccgg
     8641 gagtctcgat cgatttgtcc attcccgacg ggggaatcga tctgaacgaa gagatgaacc
     8701 gaattgagaa acgggcgatc gaaaaggcgc tgcagaagac caggggcatc aaaaccaaag
     8761 cggcggatct gctgggcgtc accagcgatt ccttaaaata ccgcatcgaa aagctgggca
     8821 ttgagtagaa aaagaacgct cttcgctcac ccgctgtttt tccgccggtt gagttccgcg
     8881 gaataagcgg caatcaaatc gtcgtggcga agcacgccca gcacaatgtt gccgtcgtcg
     8941 gggctcacca ccaggagttc ctcagcgttc gtttccatga acttcatgaa cgcgtcgtaa
     9001 aaattatcct ccggcgtcac cgttcgaacg ggactcatca tatcttcggc gatgatcaga
     9061 tcctccaggc cttcctcaaa gacaatcgtt cgcgcatgat cccagttgac ggagccgatc
     9121 agcgcgtcgt tctcgtcata aacgggaaag cattcgatgc gggaagcgga aaagattttc
     9181 ttgagctggc cgtaggacgt ggttttgcgc accggcgtaa ctttttccgg cttgtaaaag
     9241 tctctcaccc gggtttcggc aaaaagattc accgtgaaat ccgccgtgtg cgcgggcgac
     9301 tcgcgcttgc tttgaacctg ccttgcatag atggaatgct gtcgtgtaaa aagaatggca
     9361 atgatggaca ccagcatcag aggcgcgata aggccatatc cccgggtcat ctccgaacac
     9421 atcaaaagtg ttccgatggg cgcgctggcc acccccgcaa agaacgacgc catgcccaca
     9481 accacaaagg cttccggctg cgccacgata tttggataaa aataatgggc ggaatacccc
     9541 acagccccgc cgatcattcc cccgataaac agtgtcgggc cgaagacacc gccggaaccg
     9601 cccgacgata tcgtaaaact ggtggcgatg attttggcca acgcgatcgc catcatgatt
     9661 ccgatgccga gtttgcccag gatggccagt tgtatccacc cccagccgtc accataaacc
     9721 tgcgggaaaa aaaggccgat gcagcccacc gccaggccgc ccagcgccgg cttgacatag
     9781 agggggatct tcagaggctg aaaaatgcgc tcgcgtaccg cgtaaaatac cttgatgaaa
     9841 aaaatcccgg ccggcacaca aatcagaccc agaaggatat aaaaaatcag ctcgcgggga
     9901 ttggaaaacg tgtattccgg aaagaaaaaa atcggcgcgt tgccgaaaaa ataggtaaag
     9961 atgatatagg cggtaatcga actcaccacc gtgggcacga gggcttccgt ttccatgtct
    10021 tccttgtaga ggatttcaat ggcggtgagt gcgccgccga gaggagcgcg aaaaatcgcc
    10081 gaaagacctc ctccgcagcc cgccagaagc aaaatgcgcc gctcgcggtc gcttagtctt
    10141 aaaagacgcg cgatggccga accgatgccg gcgccgatct gggcaatcgg tccttctctt
    10201 cccgcgctgc cgccgctgga cagcgtgata atggaagcca gtcccttgat gaggggtaca
    10261 cgcgtccgga tgcgcccttt cttgttgtga aatgcgtcaa tcattgcgtc tgtaccgtgg
    10321 ccttcagctt ccggcgcgaa atgatagacg agcaaaccgc tgatcagacc tcccagggcg
    10381 ggcaaaagga aaaaaaccag gcgatggaat tgaagcgtgc ctgcggcttc gaagagctgt
    10441 tcacccgcag gcgccggaag ggcatacccc gccagcaggt taaaacagaa gtggcggccg
    10501 gcctggagca ggtaataaaa ggccgacgcc ccgagtcccg aaacgatgcc gaccaaaagc
    10561 ccgaacacca gccaccgcgc gatgttttcc agaaaaatac tccgcagccg atgaaccacc
    10621 tgtttcaata aaaacatcat ttccttttgt ctgtcccgct tgctgtcgat ccgttgcgaa
    10681 gaaacggtaa aacatttaac ccgatttgcc gggggatgca aatcaaaagt aaaaaactgc
    10741 tgcccgaagg caacagtttt ttaaaacgaa ccggtccggc aaaaccggac cagcggcata
    10801 aaaactttat taaccctgct gagcttaccc cttgagcggg gagtccattt ccttcacggc
    10861 caggtcctga taggcgtggg agaatctgtg gcagaaggcg tcccatcggc ggcagacatc
    10921 gatcgtggcc tcatccattc caaagaggtg cggatgcgcg accagcgctt tgatttcttc
    10981 ggccatctcc tgaacctgcg ccttgtccag tccctgaaga atttcagcga cgaattcctc
    11041 cggtttgtac cagccggctg ccttctgcag gtcgcggtgg atttgcgtca aaagaacgtt
    11101 gcgggggcct tcccacagtt cgttgatggc ggagtcacga tacaggcgcg gcaggcacga
    11161 aaaatcctcc atcacgccgt ggccgccgaa aagcgacatg gcttcacgga tgacatcggt
    11221 gcagtcccag gaggcggcga tcttctgcag catgatcagt tcgcggatat tgaaccggtg
    11281 acggcgcgcg ggaagcggtt cgtcgtcgcc ccggctgccc ttgaggccgc cttccagctt
    11341 caggaaatcg cggtagagct tgaaggcgcc ggcggtggtc cggcgggtgg ttttccggat
    11401 tctgtcgagc tggacggcga ccagaggaaa ttgattcagc cgaaggccga acgcgtcgcg
    11461 gaaggagacg tatttttccg cctcccgcac ggcccgcgtc atgtaagccg cggcggaaag
    11521 ccccaccgtc aggcgcgaat aggt
//
LOCUS       DAOSIM010000042        11462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_5612_length_11462_cov_8.805470, whole genome shotgun sequence.
ACCESSION   DAOSIM010000042 DAOSIM010000000
VERSION     DAOSIM010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 11462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11462
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5612_length_11462_cov_8.805470"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /gene="rpsB"
                     /locus_tag="PLR47_03250"
     CDS             <1..430
                     /gene="rpsB"
                     /locus_tag="PLR47_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOG11985.1"
                     /translation="AFPKKEITKLQKEMDKMDNVLGGIKNMRSLPAAVFIVDPNRERI
                     TVQEAKKLKIPLVAIVDTNCDPTDIDYIIPGNDDAIRAIKLFSSKFADAVIEGRKRFE
                     EKLSAESNKEEGAPAAENKDPEADIPESVEMENGSPAPAS"
     gene            512..1165
                     /gene="tsf"
                     /locus_tag="PLR47_03255"
     CDS             512..1165
                     /gene="tsf"
                     /locus_tag="PLR47_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOG11986.1"
                     /translation="MREKQTQTKEFQEEKIVEITSTMVKELRTKTGAGMMDCKEALAA
                     VDGDFEKAIDFLRKKGLSAATKRSSKAAKDGTIASYIHMGGRIGVMVEINCETDFVAK
                     TADFQTMARDIAMHIAASNPLYVRPDEIAEDALNREKEIYRSQLREEKKPEKIWDKII
                     EGKLKKYYEDVCLVEQKFVKNQDITVGTLISNMIAKTGENIVLRRFARFQLGEEIKQ"
     gene            1165..1890
                     /gene="pyrH"
                     /locus_tag="PLR47_03260"
     CDS             1165..1890
                     /gene="pyrH"
                     /locus_tag="PLR47_03260"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOG11987.1"
                     /translation="MSEKKKAAYQRILLKLSGEALLGKQSTGVDPDVANYIADEIRSL
                     ADLKIQIGIVIGGGNIFRGVQAGARGMERTTADYMGMLATVINSLALQSALEAKGVAT
                     RVQSSIEMHEIAEPFIQRKAVRHLEKGRIVIFAGGTGNPYFSTDTAASLRAMEIRADV
                     ILKATKVDGVYSADPVKDQSAVMFKKISYIDVLTKNLKVMDSTAISLCRDNGLPLIVF
                     NLQKKGNIRGVVCGKKIGTYVGE"
     gene            1893..2450
                     /gene="frr"
                     /locus_tag="PLR47_03265"
     CDS             1893..2450
                     /gene="frr"
                     /locus_tag="PLR47_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOG11988.1"
                     /translation="MKEQIFSKFKDNMEKTISSLEKSFSRVRTGRASVSLLDGIKVDY
                     YGTPTPIAQVANLSVPESRLIVIAPWDVTTIGAIEKAIQKSDLGLMPSTDGKVIRLSI
                     PQLTEERRKDLVKVVKKMAEEGKIKLRNERRDANEALKELKKNNKMSEDEMHGAQDEV
                     QKLTDRYIEKTDKILAAKEKEIMEI"
     gene            2526..3266
                     /locus_tag="PLR47_03270"
     CDS             2526..3266
                     /locus_tag="PLR47_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOG11989.1"
                     /translation="MPEIDQNQLPRHIAIIMDGNGRWAKQHAMGRIRGHKKGAQAVRT
                     TVTACRELGISCLTLFAFSSENWGRPGEEVSALMNLLADYLDKEAPTMQKQEIRLTTI
                     GDIDRLYAPVREKLLEVQKLTRQNNKMVLNLALSYGSRDEILAAVKKMLADSREGKLS
                     PDSVDQDVFERYLYTSGMPDPDLLIRTSGEYRISNFLLWQMAYTELYFTDVLWPDFKK
                     DDLLKAIAAYQSRERRFGLTSDQVSGRK"
     gene            3286..4101
                     /locus_tag="PLR47_03275"
     CDS             3286..4101
                     /locus_tag="PLR47_03275"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOG11990.1"
                     /translation="MATHNSNLQRWMTGLALAAVLLLIIVMGSPVVLTAIVALVIVIG
                     LWEYNGIVFGPGHRAEKTESLLFAFLIPWAVLAGDARWIAAILTFAMLIVFMVFLWQV
                     RKSFPDPSSVMKVMFGLLYIPLLTSHFVLLRKLEGGIYWVLLVLVIGIIGDTVALYVG
                     KSLGKRKLLEAVSPGKTVEGTLGLIAGSTLAACVFGYFWLPGVHWIHFPVLGFVGGII
                     GQLGDLCESAIKRSHGKKDASDLLPGHGGLMDRMDSLIFLAPFVYYYRIFVMG"
     gene            4101..5267
                     /locus_tag="PLR47_03280"
     CDS             4101..5267
                     /locus_tag="PLR47_03280"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOG11991.1"
                     /translation="MKKITILGSTGSIGASALDVVRKNPNKFRVAALAAGRNVKLLAR
                     QALAFHPAAVSVRTPGDAKRLRGLLGPGGKIRVLCGEEGVREIAAHSAADIVLSAISG
                     AAGLKPTLAAIEAGKDIALANKETMVIAGHLVTKKAEQKKIRVLPVDSEHSAIFQCLA
                     GQKPANIKRIILTASGGPFYSFSRADLKKVTPAQALRHPRWKMGAKITVDSASLMNKG
                     LEVIEAKWLFDIAVSRIDVLIHPQSVVHSALELLDGSVLAQMGIADMRIPIAYALSFP
                     DRMTNDLPALDLAKAGPLSFFPPDTKKFPCLRLALEAARIGGTAPAVLNAANEEAVAA
                     FLENKICFHEIPNVIEKTLERHSAIPDPSLEEILLADGEARKVAAGILKKRKRG"
     gene            5272..6363
                     /gene="rseP"
                     /locus_tag="PLR47_03285"
     CDS             5272..6363
                     /gene="rseP"
                     /locus_tag="PLR47_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOG11992.1"
                     /translation="MVTFISFIVLLGILIFAHELGHFLAARAGGVGVLKFSLGFGPKI
                     FGKKIGETEYVLSWIPLGGFVKLLGESGAEELPAEDEKRSFYKQPRWKRMLIVLAGPV
                     FNFLLAVFIFFLVYMHGLPNLLPVIGELSPDSAAASSGILKDDAILSINDREITFWDE
                     IKPVILKAGGADLTIRIRRGGEEKIFAVKPKLTKTKNIFGEEESTYLIGISPAGKTVI
                     EKKNPTAAMASAVEKTWEISKLTVISVVKMLEGVLSPRTLGGPIFIAQVAGNQVREGI
                     IPFVLFMAILSINLGVINLFPIPVLDGGHIFFYLIEMAARREIPVKIKEWASQIGFVF
                     LLMLMLFVIMIDIERLNIGPVNKVLQFFR"
     gene            6365..7057
                     /gene="tsaB"
                     /locus_tag="PLR47_03290"
     CDS             6365..7057
                     /gene="tsaB"
                     /locus_tag="PLR47_03290"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOG11993.1"
                     /translation="MLTLAFDTSSKTVSVALLEDRAVLYNAILHREQNHSEVLLPAID
                     EACRQTGISVRDIGFFACTLGPGSFTGLRIGLSTLKGLMMATGKPAAGVSSLAALALN
                     AEEPDAVIGSMMDAGRGQVYLAYYRRGKDGVLQPVRAPQAQDPRSVEDPFSEPVIYVG
                     DGAVRYAGLIPPLSSPKNKMAPVEQQFISAAAVARLALEKYFRKDFLDPARCAPLYLR
                     SVDALPVAGSSG"
     gene            7141..7965
                     /locus_tag="PLR47_03295"
     CDS             7141..7965
                     /locus_tag="PLR47_03295"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HOG11994.1"
                     /translation="MTRNIPKIFAALLLLSLCVLLPCLTGAERIPPGKNPFSLLMKTK
                     APISAVAVQKIHPHDSDAFTQGLIVMDGVLCESTGLLGRSTLACKDIDTGKVLRQIQL
                     PPEFFGEGIALLKGRIYQVTWQNENALVYDAASLREIRRMKYSGEGWGLTSDGKSLLM
                     SDGSSIITIRDPKSFQVQRRIRVQDGDRPVSRLNELEFVRGEIWANIYMEDLIARISP
                     QTGRITGWIDLSALRSYLPANARVDVLNGIAFDAKQNRIWVTGKYWPHLFEIQITP"
     gene            8045..8692
                     /locus_tag="PLR47_03300"
     CDS             8045..8692
                     /locus_tag="PLR47_03300"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOG11995.1"
                     /translation="MRIILLGAPGAGKGTIAKLLTEYDGSIQISTGDILRSAVKEGSA
                     LGKKAKEYMDRGDLVPDSLIMDIMEARLQEPDCQKGFILDGFPRTIPQAEDLQKLLSK
                     LKIKLDFVANLDVPRDVILDRLTTRRTCCNPACQAIYNVKSNPPREDGTCTLCDHQTI
                     QRDDETEEAITNRLETYNLKTAPLTGFYEKEGLLKTFLSLSSKDTVAEIKKALHA"
     gene            8707..10671
                     /locus_tag="PLR47_03305"
     CDS             8707..10671
                     /locus_tag="PLR47_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOG11996.1"
                     /translation="MIEYAKELNPEQYRVVTEEGGPLLVLAGAGSGKTRTLTYRVAHL
                     LESGIRPENILLATFTNKAARSMLNRVESLVQHYTGKIMGGTFHSIAHRILRSYAFRL
                     GYNSSFSIVDSEDARQVIGSAMAELKIDTKLTKFPKNNIIAEMISLALNTETALEDVI
                     SSRYPFFLHLSAEIHQIACLYDQKKKDLSVMDFDDLLANCRRLLREQPDILQTLSRRF
                     EHVLVDEYQDTNILQADIVDLLASGHRNLMVVGDDSQSIYSFRGANFENIIHFPERYP
                     DCKIFKLETNYRSTPEILHLANLSINNNENQFPKALKAVRNKGMRPVIVSAQNVLKQS
                     DFVAQRISELSRSGVPFCEMAVLYRAHYHSMEAQMEFVRRDIPFDIRSGIRFFEQAHI
                     KDVTSYMRVLVNPHDELAWRRLLMMYPKIGKITFEKIWSRLKKQDHPLETLISESFLQ
                     TAPKSAQNGVGQCRKTLLLLLALDVPDRTPEKIIDLLLNKGDYRTYLQDSYSDAYARE
                     EDLIQLGNFAARFERMDDFLNELALLTNMSKEEEIEERQEDKVILSTIHQAKGLEWSY
                     VFIIGCADGMIPLERALREPGGEEEERRLFYVAVTRAKDQLYLSYPVLDYSRSAGVQT
                     LCPSRFLSEISPLSPQDEHRPFEQWALFDD"
     gene            complement(10702..11202)
                     /locus_tag="PLR47_03310"
     CDS             complement(10702..11202)
                     /locus_tag="PLR47_03310"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOG11997.1"
                     /translation="MKEMEYLNAAGEAMRKIRKGAFLTVRAADAQNTMTIGWAMLGVI
                     WQKPVLMVAVRNTRHTFGLMEKAPDFTVTVPSGDMSRETAFCGTKSGRDLDKFAMCPL
                     KTAKGRHVASPIIQTPGMHFECKKMYQSAMNPAHLDSGLDQAIYPKKDYHTLYFGEIL
                     ACYETE"
BASE COUNT         2710 a         3014 c         3226 g         2512 t
ORIGIN      
        1 ggcttttccc aaaaaggaaa tcaccaagct gcaaaaagag atggacaaga tggacaatgt
       61 tctgggcggc attaaaaaca tgaggtccct tcccgccgcc gttttcattg tcgatcccaa
      121 ccgggagcgc atcacggtcc aggaagcgaa aaaactgaag attcccctgg tcgccatcgt
      181 cgataccaac tgcgatccga cggacatcga ttatatcatt ccgggaaacg atgacgcgat
      241 tcgggccatc aagctgttct cgtcgaagtt cgccgacgcg gtgatcgaag gcaggaaacg
      301 ctttgaagaa aagctgtccg cggaatccaa caaggaagag ggagcgcccg ccgcggaaaa
      361 caaagatccg gaagcggaca ttccggaaag cgtcgagatg gaaaacggct ctcccgcgcc
      421 cgcttcatag aagcatacac cgcatacacg cgaaaagcac accccgcaag gcgatggaag
      481 gtgccgccgt tttcggcgat cagacgctcg catgcgtgaa aagcaaacac agaccaaaga
      541 attccaggag gaaaagatag tggaaatcac gtcaaccatg gtaaaagaat tgagaacaaa
      601 aaccggcgcc ggcatgatgg actgcaagga agcgcttgcg gccgtggacg gcgattttga
      661 aaaagcgatt gactttttga gaaagaaggg gctttcggcg gcaaccaaac gctcgtccaa
      721 ggcggccaag gacggaacca ttgcttccta tatccacatg ggcggccgga tcggcgtgat
      781 ggtggaaatc aactgcgaga cggattttgt ggcgaagacg gcggactttc agaccatggc
      841 gcgggacatc gccatgcaca ttgccgcatc caatcccctg tacgtgcgtc cggatgaaat
      901 cgccgaagac gcgctcaacc gggaaaagga aatttaccgc agccagttgc gcgaagaaaa
      961 gaagccggag aaaatctggg acaaaatcat cgaaggaaaa ctgaagaaat attacgaaga
     1021 cgtttgcctg gtcgaacaga aattcgtcaa gaaccaggac atcaccgttg gaacgctgat
     1081 cagcaacatg attgccaaaa caggcgaaaa tattgttctt cgccgttttg cccgcttcca
     1141 gctcggcgag gaaatcaagc aataatgagc gaaaagaaaa aggccgcata ccaaagaatc
     1201 cttttaaagc tcagcgggga agctcttctg ggcaaacagt ccacgggcgt tgatccggat
     1261 gtggcaaatt acattgcgga tgaaatccgg tctctcgcgg acctgaagat tcagatcggc
     1321 attgtcatcg ggggcggaaa tattttccgg ggcgtgcagg ccggcgcccg gggcatggag
     1381 cgcacgaccg ccgattacat ggggatgctg gcgacggtca tcaacagcct tgcccttcag
     1441 agtgcgctgg aagcaaaggg tgttgcgacg cgcgtccagt cctccattga aatgcatgaa
     1501 atcgcggaac cttttattca gcggaaagcc gtccgtcatc tggaaaaggg caggattgtc
     1561 attttcgccg gcggaacggg aaatccctat ttttccacgg acacggccgc atctctgcgg
     1621 gcgatggaaa tccgggcgga tgtgatcctg aaggcaacga aagtggacgg ggtctacagc
     1681 gcggatcccg tcaaagacca atctgcggta atgtttaaaa aaataagtta tatcgatgta
     1741 ttgaccaaaa accttaaagt aatggattcg acggctattt ccctttgtcg cgacaacggc
     1801 ctgcccctca tcgtctttaa cctgcagaag aagggcaaca ttcgcggcgt tgtctgcggt
     1861 aaaaaaatcg gaacgtacgt aggagaatga tcatgaaaga gcagattttt tcaaagttca
     1921 aagacaatat ggaaaaaacc atctcttccc tggagaaatc attcagcaga gtaagaaccg
     1981 gccgggcgtc ggtttccctg cttgacggca tcaaggtgga ctattacgga acgccgactc
     2041 cgattgccca ggtggccaat ctttccgttc cggaaagccg cctgatcgtc atcgctccct
     2101 gggatgttac gacgatcggc gccatcgaaa aggccattca gaaatccgac ctggggctga
     2161 tgccctcgac ggacggcaaa gtgattcgcc tttccattcc gcagctcacc gaggagcggc
     2221 gaaaggacct ggtcaaggtt gtgaagaaaa tggcggaaga ggggaaaatc aagctgcgca
     2281 acgagcggcg cgacgccaat gaagcgctca aagaattgaa aaagaacaac aaaatgtccg
     2341 aagacgaaat gcacggcgcc caggatgaag ttcagaaact gacggaccgt tatatcgaaa
     2401 agacggacaa gattctggcc gccaaagaaa aagaaatcat ggagatttaa attggtcaga
     2461 cgataaacct gaacaatggg gagtcaaagc tccccatttg tttttaaagg acaaagcaag
     2521 ttcaaatgcc tgagattgat caaaatcaac tgccccgcca catagcgatt attatggacg
     2581 gcaacggccg ctgggccaag cagcacgcca tggggcggat ccgggggcat aaaaagggag
     2641 cgcaggctgt ccggacaacg gttacggcgt gccgtgaact ggggatttcc tgtcttaccc
     2701 tgttcgcttt ctcctcggaa aactggggcc gcccgggcga agaagtcagt gcgctgatga
     2761 atctccttgc ggattacctg gacaaggaag cgccgacgat gcaaaagcag gaaatccgcc
     2821 tgaccactat cggcgacatc gatcggctct acgcgcccgt gcgcgaaaaa cttctggagg
     2881 ttcagaagct gacacggcaa aataacaaga tggttttgaa tttggcgctg agctacggca
     2941 gccgggacga gattctcgcg gccgtcaaaa aaatgctggc ggacagccgg gaaggaaaat
     3001 tgagccccga ttccgtggat caggatgtgt ttgaacgcta tctgtacacg agcggaatgc
     3061 ccgaccccga cctgctgatc cggacgagcg gcgaatatcg catcagcaat tttcttctct
     3121 ggcagatggc ctatacggag ctttacttta cggatgtttt gtggcctgat ttcaaaaaag
     3181 acgatttatt aaaggcgatt gccgcatatc agagccggga aagacgtttc ggcttaacca
     3241 gtgatcaggt ttccggcagg aaataacggt ctaaaggagt gagagatggc gacccataac
     3301 tccaatctgc agcgatggat gaccggtctg gcgctggcgg ccgtgctgct cctgatcatt
     3361 gtcatgggat cgccggtcgt cctgaccgcc atcgttgcgc tggtgatcgt catcggcctt
     3421 tgggaataca acggcattgt gttcggcccc gggcatcgcg cggaaaaaac cgagagcctg
     3481 ctcttcgcct tcctgatccc ctgggccgtg ctcgcagggg atgcgcggtg gatcgccgcg
     3541 attctgacct tcgcgatgct gatcgtgttc atggtttttc tgtggcaggt ccgaaagtct
     3601 ttcccggatc cgtcctccgt catgaaggtc atgttcggcc ttctgtacat tccgcttttg
     3661 acctcgcact tcgtcctgct gagaaagctg gagggcggca tttactgggt tcttctggtg
     3721 ctcgtcatcg gcattatcgg ggacacggtg gctctgtacg tgggcaagtc cctgggaaag
     3781 agaaaactgc tggaggcggt gagtccgggg aaaaccgttg aaggcacgct ggggctcatc
     3841 gcgggctcca ccctggccgc ctgcgtgttt ggctatttct ggcttcccgg tgtccactgg
     3901 attcattttc cggtcctggg ctttgtgggc ggcatcatcg gacaactggg cgatctttgc
     3961 gaatccgcca ttaaaagaag ccacggcaag aaggacgcca gcgacctgct gccggggcac
     4021 ggcggactga tggaccggat ggacagcctg atcttcctgg cgccttttgt gtattactac
     4081 cgcatctttg tgatgggctg atgaaaaaaa taacgatcct gggctcgacc ggatccatcg
     4141 gcgcaagcgc gctggatgtc gtccggaaaa atccgaataa atttcgcgtg gcggcgctcg
     4201 ccgcgggccg caatgtgaag cttctggccc gccaggcgct ggcatttcac cccgcggcgg
     4261 tttccgtgcg gacgcccggg gatgcaaagc gccttcgcgg tcttttggga ccgggcggca
     4321 aaattcgcgt gttgtgcggg gaagagggag tccgggaaat tgcggcgcat tcggcggccg
     4381 acatcgttct ttccgccatt tccggcgcgg cgggcctgaa gcccacgctc gcggcgatcg
     4441 aagcggggaa agacatcgcg ctggccaaca aggaaaccat ggtcattgcc gggcatctgg
     4501 tgacgaaaaa agccgaacaa aaaaagattc gcgtgcttcc cgtggacagc gaacacagtg
     4561 cgatctttca atgcctggcc ggacagaagc cggcaaacat caaaaggatt attctcaccg
     4621 cgtcgggcgg tccgttttat tccttttcgc gggcggatct gaaaaaagtg acgccggcgc
     4681 aggcgctcag gcatccgcgc tggaaaatgg gcgcgaaaat tacggtggat tcggcgtcgc
     4741 tgatgaacaa aggcctggag gtaatcgagg cgaagtggct gttcgacatt gctgtttccc
     4801 gaatcgatgt tttgattcac ccgcagagcg ttgttcattc ggcgctcgag cttctggacg
     4861 gatccgttct ggcgcagatg ggcattgccg acatgaggat tcccatcgcg tacgcgctgt
     4921 catttccgga ccggatgacg aacgaccttc cggcgctgga tctggccaaa gccggcccgc
     4981 tttcgttttt cccgccggac acgaaaaaat ttccctgcct gcgcctggct cttgaggccg
     5041 cgcggattgg aggcacggcg ccggcggttc tgaatgcggc gaatgaagag gcggtggccg
     5101 cttttcttga aaataaaatt tgctttcacg agattcccaa cgtaatagaa aaaacgctgg
     5161 agaggcattc ggccataccg gacccttcgc tggaggaaat tctgctggcg gacggtgaag
     5221 ccagaaaggt cgcggccggc attttgaaga agcgcaagag aggatgatgg tttggttact
     5281 tttatttcgt ttattgtgtt gctggggatt ctgattttcg cgcatgaact ggggcatttt
     5341 ctggccgcgc gggccggcgg cgtcggcgtc ctgaagtttt ccctgggatt cggacccaaa
     5401 attttcggga agaaaatcgg cgaaacggaa tatgtccttt cctggattcc gctgggcgga
     5461 ttcgtcaaac tgctcggcga atcgggcgcc gaggaactgc cggcggaaga tgaaaaaagg
     5521 tctttttaca agcagccgcg gtggaagcga atgctcatcg tgctggccgg ccccgtcttc
     5581 aactttcttc tggccgtctt tatttttttt ctggtttaca tgcacgggct gcccaatctt
     5641 cttccggtca tcggggagct gtcgcccgat tccgccgccg cctcgtcggg cattctcaaa
     5701 gacgatgcca ttttgtccat caatgaccgc gaaatcactt tctgggacga gatcaaaccc
     5761 gtcatcctga aagcgggggg cgccgatctc accattcgaa tccggcgcgg cggggaagag
     5821 aaaatctttg ccgtgaaacc gaagctgacc aagacgaaaa acattttcgg cgaagaggaa
     5881 tccacctacc tgatcggcat ttcaccggcc ggaaagaccg tcattgaaaa gaaaaatccg
     5941 acggcggcca tggcttcagc ggtggagaaa acctgggaaa tcagcaagct caccgttatt
     6001 tccgttgtga aaatgctgga gggggttctg tcgccccgaa cgctgggcgg acccatcttc
     6061 atcgcacagg tggcgggcaa ccaggtccgt gaaggcatta ttccgtttgt cctttttatg
     6121 gcgattcttt cgatcaatct gggggtcatc aatttgtttc ccatccccgt gctggacggg
     6181 ggacacattt tcttttatct tatcgaaatg gcggcccgaa gggaaattcc ggtcaaaatc
     6241 aaggaatggg cctcgcagat cgggtttgtc tttttgctga tgctgatgct tttcgtcatc
     6301 atgatcgaca tcgaaaggct caatatcgga ccggtcaaca aggtcttaca atttttcagg
     6361 taatatgctg accctggctt tcgacacgtc ttccaaaacc gtttcggtcg ccctgctcga
     6421 agaccgggcg gttttataca acgccattct tcatcgggag caaaaccatt cggaagtgct
     6481 gcttccggct attgacgagg cctgccggca gacggggatc tccgtgcggg acattggatt
     6541 ttttgcctgc acgctggggc ccggctcctt taccggactg cgcatcggcc tcagcacact
     6601 caaaggtctg atgatggcga cgggaaaacc ggcggcggga gtttcctcgc ttgcggctct
     6661 ggcgctcaac gctgaggagc cggacgccgt gatcggttcg atgatggatg cgggccgcgg
     6721 ccaggtgtat ctggcctact accggcgcgg aaaggacggc gtcctccaac cggtcagggc
     6781 gccgcaggct caggacccgc gtagcgtgga ggatcctttt tccgagccgg tcatctatgt
     6841 cggagacggc gccgtccgct atgccgggct gattcctccc ctgtcttcgc cgaaaaataa
     6901 aatggcgccg gtcgaacagc agtttatcag cgccgccgcc gtcgcgcggc tggcgctgga
     6961 aaaatatttc cgcaaggatt ttctggatcc ggcccggtgc gcgccccttt atttgcgttc
     7021 ggtggatgcc ctgccggttg caggcagcag cggatgacgg cgcggccggt cgcacagaca
     7081 cgaggatgtc cccgtgagct gagggaaatg cttttccctc agcccggacg gtaattcaag
     7141 atgacccgaa atatccccaa aatatttgcc gcattgctgc tcctttcgct ttgtgttttg
     7201 ctcccgtgcc tgacgggcgc ggagaggatt ccccccggca agaatccgtt ttcccttctg
     7261 atgaaaacga aggcgcccat ctccgccgtc gcggttcaaa aaatccatcc gcacgattcg
     7321 gacgccttca cgcagggact gatcgtcatg gacggtgttc tgtgcgagtc caccggtttg
     7381 ctgggccgat cgacgctggc ctgcaaagac atcgataccg ggaaagttct caggcagatt
     7441 caactgccac cggagttttt cggagagggc atcgcgcttt tgaaaggcag gatttatcag
     7501 gtcacctggc aaaatgaaaa cgccctggtg tacgatgccg cctccctgcg ggaaatcagg
     7561 cgaatgaaat attccgggga aggctgggga ttgacttccg acgggaaaag cctgctcatg
     7621 agcgacggct cttcaatcat caccatccgt gacccgaaat cctttcaagt ccaaagaagg
     7681 attcgtgtcc aggacggcga ccggccggtt tcccgcctca atgaactgga gtttgtccgg
     7741 ggggaaatct gggcgaatat ttacatggaa gatcttattg cccgtatttc tccccaaacc
     7801 ggcaggataa cgggctggat cgatctttcc gcgcttcgct cctatctgcc cgccaatgcc
     7861 cgcgtggatg tgctcaacgg catcgctttt gacgcaaaac aaaaccggat ttgggtgacc
     7921 ggcaaatact ggccgcacct ttttgaaatt caaatcactc cctaaaacct taattgcctt
     7981 gaccgtttga aggttttgca ctataagcgc cgcagcttaa acatttgatg gatggaggat
     8041 tgcaatgaga attattttac tgggcgcgcc gggcgccggc aaaggcacga tcgccaagct
     8101 gctgaccgaa tatgacggat ccatacagat ctccacgggc gatattctgc gctccgccgt
     8161 gaaagagggc agcgccctcg gcaaaaaagc caaagaatac atggatcgtg gcgacctggt
     8221 gccggattcg ctgatcatgg acatcatgga ggcgaggctt caggagccgg attgtcaaaa
     8281 gggatttatt ctggacggtt ttccgcgtac gattccgcag gccgaagacc ttcagaaact
     8341 gctgtccaaa ctgaaaatca agctggattt tgtcgccaat ctggatgtgc cgcgcgacgt
     8401 gattctggat cggctgacga cccgcaggac ctgctgcaac cccgcctgcc aggccattta
     8461 caacgtcaaa agcaatccgc ccagagaaga cggcacctgc acgctttgtg accatcagac
     8521 cattcagcgc gacgacgaaa ccgaagaggc gatcaccaac cgtctggaaa cctacaattt
     8581 gaaaacagcg ccgctcaccg gtttttatga aaaagaaggg ttgttgaaaa cctttttgtc
     8641 gctcagctct aaagatacgg ttgctgaaat taaaaaggcc cttcatgcct gagccctcga
     8701 cagtcgatga ttgagtatgc caaagaactg aatccggagc aataccgggt tgtcacggaa
     8761 gaaggcggtc cgcttcttgt tcttgcggga gccggaagcg gcaagacgcg gacgctgacc
     8821 taccgggtgg cgcatcttct ggaatccggc atacggccgg aaaatattct tctggcgacg
     8881 tttacaaata aggccgcccg atccatgctg aaccgcgtgg aaagcctggt tcagcattac
     8941 accggcaaaa tcatgggcgg cacgtttcat tccattgccc atcgtatttt gagaagctac
     9001 gcttttcgtc tcggctacaa cagcagtttt tccatcgttg acagtgaaga tgcccgacag
     9061 gtcatcggat cggcgatggc cgaattaaag atcgatacca aactgaccaa attccccaaa
     9121 aataatatta ttgcggaaat gatcagtctg gcgctgaaca cagagactgc gctggaagat
     9181 gtgatttcgt cgagataccc cttctttttg cacttaagcg cggaaattca tcagatcgcc
     9241 tgcctctacg atcagaagaa aaaagatcta tcggtaatgg attttgatga tctgctggcc
     9301 aactgccgca ggctgctccg ggagcagccc gatattttac agacgctttc ccggcggttt
     9361 gagcatgtcc tggtggatga atatcaggat accaacattc tgcaggccga catcgtcgat
     9421 ctgctggcgt ccgggcatcg caatctgatg gtggtgggag acgattcaca aagcatttat
     9481 tcttttcggg gcgccaattt cgagaacatc atccattttc ccgagcgcta tcccgactgc
     9541 aaaatattca aactggagac caactatcgc agcacgccgg aaattctgca tctggccaac
     9601 ctgagcatca acaacaatga aaatcagttt cccaaggcgc tgaaagccgt tcgcaacaag
     9661 ggcatgaggc cggttatcgt ttccgctcaa aatgttttga agcagtcgga cttcgtcgcg
     9721 caaagaatct cggagctctc ccgcagcggg gtgccgtttt gcgagatggc cgtgctctac
     9781 cgggcgcact atcattcgat ggaagcgcag atggaatttg tgcggcgcga cattccattc
     9841 gacatccgct ccggcatccg tttttttgag caggctcaca tcaaagacgt gacatcgtat
     9901 atgcgggttc tggtgaatcc ccatgatgaa ctggcctggc gaaggctgct gatgatgtat
     9961 cccaagatcg gcaagattac ctttgagaaa atctggagcc ggcttaaaaa gcaggatcat
    10021 ccattggaga ctttgatttc ggaatcgttt ttacaaaccg cgcccaaatc ggcgcaaaac
    10081 ggcgtaggtc agtgccggaa gaccttgctg ctattgcttg cgctggacgt ccccgacaga
    10141 acaccggaaa agatcattga tctgctttta aacaaaggcg attaccggac ctatctgcag
    10201 gacagctatt ccgatgcgta cgcccgtgaa gaggacttga tccagctggg gaattttgcg
    10261 gccaggtttg agaggatgga tgatttttta aacgaactgg cgctcttaac caatatgtcc
    10321 aaagaagaag aaatagaaga gcggcaggag gacaaggtca tcttaagcac gattcatcag
    10381 gccaaagggc tggaatggtc ttatgttttc atcatcgggt gcgccgacgg catgattccc
    10441 ctggagcggg cgcttcggga gccgggcgga gaagaagaag aaagaagact cttttacgtg
    10501 gcggtgacgc gcgccaaaga ccagctttac ctttcgtatc ccgttttgga ctacagccgc
    10561 tccgcgggcg tgcaaaccct ctgcccgtcg cgttttctaa gcgaaatatc gcctctctcc
    10621 ccgcaggacg aacatcgccc ctttgaacag tgggcgctgt ttgacgatta agggcgtccc
    10681 tgcggccgga aatgccgccg cttattcggt ctcgtagcag gccagaatct cgccgaagta
    10741 cagcgtatga tagtcttttt tcggatagat ggcctggtcc aggcctgaat ccagatgggc
    10801 cggattcatc gcggactggt acattttttt gcactcgaaa tgcattcccg gcgtctgaat
    10861 aatcggggac gcgacgtgcc gcccctttgc cgttttgaga ggacacatcg cgaatttgtc
    10921 caggtcgcgg cccgatttgg tcccgcagaa agccgtttcc cggctcatgt cgcccgaagg
    10981 aacggtgacc gtaaaatccg gcgccttttc cataaggccg aaagtgtggc gggtatttcg
    11041 caccgccacc atcaggaccg gcttctgcca gatgacgccg agcatggccc agccgatggt
    11101 catggtgttt tgcgcatccg ccgcgcgaac ggtcagaaaa gctcctttgc ggattttgcg
    11161 catggcttcg cccgccgcgt ttaaatattc catttctttc atgctgcctc ccttttttct
    11221 cacggcacgg cggctgtctt tcttttcaag cttcaaatct cgaaagcctc cgcgtttttg
    11281 attcaatagc aacctttgag gctcccggca acgttttttt cctcaaatct ttcttgaaac
    11341 gccgggcgct ttggactatg aagattggac aaaagggagg atggttcacg ttgatcgatc
    11401 agattgcggc ccgctgcctg aaatgcaagg ttccccagtg caggaagcac tgccccgtat
    11461 cc
//
LOCUS       DAOSIM010000043        11250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_5722_length_11250_cov_7.542296, whole genome shotgun sequence.
ACCESSION   DAOSIM010000043 DAOSIM010000000
VERSION     DAOSIM010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 11250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11250
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5722_length_11250_cov_7.542296"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLR47_03315"
     CDS             complement(<1..195)
                     /locus_tag="PLR47_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11998.1"
                     /translation="MKRHQNDKRRHAQNGNQQLSFEFHILSDPGVHVLRKNLPMDNHV
                     NDSRHQEKKGSRVMDGHEKVA"
     gene            347..490
                     /locus_tag="PLR47_03320"
     CDS             347..490
                     /locus_tag="PLR47_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG11999.1"
                     /translation="MIRASTMKDFPYGLKLAEAIEPLFGPVIDDPAFCDGLKQVIRGH
                     LQT"
     gene            570..980
                     /locus_tag="PLR47_03325"
     CDS             570..980
                     /locus_tag="PLR47_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12000.1"
                     /translation="MKTNKIRFLLAAMLSFVFSILYNGIVHLVILGEANQQVESLRRV
                     DFSSKVWISILATLAVSFLFTALYSFFVSEKSIRKGLFFGFVFGLLIAVIVDINQYVL
                     YPLPFLLVAKWAWFGMMEFSLMGMIVGSIVKDKT"
     gene            1206..2045
                     /locus_tag="PLR47_03330"
     CDS             1206..2045
                     /locus_tag="PLR47_03330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG12001.1"
                     /translation="MNLTVSSMNSEEVALAVDWAAREGWNPGLHDAACFYAADPTGFF
                     LARFGGKPVGCISAVAYDERFAFAGFYIVETEYRTQGIGHALVEKAQDHVGGRIIGND
                     AVVAQQETYRRLGFAVAHRNIRFQGKATRALLRPSEIVDLSVVPLDDLLAYDRKMFPA
                     PRTSFLRQWIRQPEGAALGHVKKGRLSGYGVIRKCRRGYKIGPLFADDEETAGILFGA
                     LTETAAGHSFCLDIPEPNAGAQRLVTRHGMEKVFETARMYRGPAPILPLEKIFGVTSF
                     ELG"
     gene            complement(2065..2340)
                     /locus_tag="PLR47_03335"
     CDS             complement(2065..2340)
                     /locus_tag="PLR47_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12002.1"
                     /translation="MTPTTTGLIGFAVLIILMFWRMPVGFAMALTEMKKYKYDPALAT
                     GVVAGAARDVPLHIVFKGSLNMLYAMIALAVLLMVFPKIATFLPQLL"
     gene            complement(2347..2670)
                     /locus_tag="PLR47_03340"
     CDS             complement(2347..2670)
                     /locus_tag="PLR47_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12003.1"
                     /translation="MADQNLPHVAGKHCLFNGLKTSIKRRNRHEMHWNNHIIQALRRI
                     KTMTSLGYELLIFLAIAIVIAVAFIVVRPGNKSDHGTNDSENKMVDPELPSPNSKGKT
                     NHQKK"
     gene            complement(2712..3047)
                     /locus_tag="PLR47_03345"
     CDS             complement(2712..3047)
                     /locus_tag="PLR47_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12004.1"
                     /translation="MKIFRSIKNTKAFSLFLAVLLSSAVTHGVCNFGYGPEKVVVIQN
                     GSYSADGNAENNCRMKPHFPGIVFKHYGIFHNARLSDYTHLSVQYPSPKDVFLSRKST
                     RAPPARIVS"
     gene            3269..4672
                     /locus_tag="PLR47_03350"
     CDS             3269..4672
                     /locus_tag="PLR47_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOG12005.1"
                     /translation="MRPIREILEAQSVVVIGASRDQEKPGAQLLKVLMEVGFAGRVAG
                     VNPQGGEVFGLPLYSSVRDVPFDIDLAVLLIPPRFVPDALRDCAARGVKGAVISAEGF
                     AETGTEGAAYQEDVRAILKSSGMRGFGPNTLGLVNTKTKLTTSYFANSRMLAPGSIGF
                     AAQSGIFVGALLRYLSSLDGLHLSKGIGLGNKVDVNESDALSFMADDEQTKIIGLYLE
                     DVKDGNRFAKTLKKAAAKKPVLIIKSGRTRAGAQASASHTASMAVEDAVFDGIVRQAG
                     AIRLHAIDEFVRTLKGFLNMPLPLGDRLAYVSYSGAQAIMSIDTTMEQGLQTAKLADA
                     TLARIGRVIATSSKTKNPVDIFPDWLTHGYEKTITEVVKALLDDRGVDGIVFISFAET
                     NPDVYQSLIDVVQSDRRKPVFVSLLGDSRDKAACEHLLEAGGLPCCDFPEQAVRVFAR
                     MRQYAGKTGNPNSRGSS"
     gene            complement(4669..6447)
                     /locus_tag="PLR47_03355"
     CDS             complement(4669..6447)
                     /locus_tag="PLR47_03355"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOG12006.1"
                     /translation="MTSEMLIVLGVILLAIFLFASEKLPVDLTSILVMAVLIISGILT
                     PTEGLSGFSNVATVTVGAMFILSAALQKTGAVTFLGTLSSKIFRFNFWVGLVGTMVLV
                     GVVSAFVNNTPVVAVFIPIMLGLAATNKLSPSRLLMPMSFASMFGGVCTLIGTSTNIL
                     VSSIAVQHGLPALGMFEFTKLGLVFFAAGTLYMALVGVRLIPPHPAEDDLTDKYRMQN
                     YLTDIVLQDKAKSVGHRVADSPLVKELDIDILEVIRHGHRLLVPVSDIVLAAGDLLRV
                     RCDIRQLQKLKERMGITMKSDCELHDNDFRCEELRLTEVVVAPNSRLIGKTIKSSAFR
                     NIFRATALALRHNGQLFNTGFADTPLKAGDAILVETRRENYEALKSNNDFVIVSDIET
                     PQYRKSKILPAVLIILAVIVTAALGIVPIMVGAIVGAILLVVAGCITLEEAYDAIDWK
                     VIFLLGGVISLGVALDKTGAAMYLSGKMIAFLGGLGPVAIVAALYLTTTLMTEMMSNN
                     ATAVLLAPIAIAAAKAMGVSPTPFLMAIAFAASASFMTPVGYQTNTMIYGVGRYKFSD
                     FIKVGTPLNIIFWILATLLIPVFFPF"
     gene            complement(6819..7559)
                     /locus_tag="PLR47_03360"
     CDS             complement(6819..7559)
                     /locus_tag="PLR47_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG12007.1"
                     /translation="MPTYDGYGLSETTPVISNNSPKRGNKYGTVGKPFKDTNVVIDKS
                     RVGEDSPDGEIVVYGAQVMQGYHNKPAITREAMMPDTWKGFPGVRTGDRGWLDEDGYL
                     HITGRFKDEYKLENGKYVHPESIENEMKLLRYVSNAIVYGDGRLYNVALVVPDFEALK
                     TDPKTSAWAQGSREETIANKECTDFISKEIKDHLRKTFGGYEIPEKFLYITEDFTVDN
                     GMMTQTMKLIRRNIMKTYGDRLIALYSA"
     gene            complement(7706..8596)
                     /locus_tag="PLR47_03365"
                     /pseudo
     CDS             complement(7706..8596)
                     /locus_tag="PLR47_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            complement(8737..9696)
                     /locus_tag="PLR47_03370"
     CDS             complement(8737..9696)
                     /locus_tag="PLR47_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOG12008.1"
                     /translation="MIRGMERKQIFRNNDDRENFLARLEKLLPATQTSCFAWALISNH
                     AHFLFRSGTVPIATLMSRLLTGYAVYFNARHKRSGRLFQNRYKSILCQEDTYLTELVR
                     YIHLNPLRSGLVKSIEELNHYPYCGHSVLMGHKKRNWQEKDYVLSYFGKNRASAIRRY
                     NEFVAAGIEQGRRTDLIGGGLIRSLGGWSPINKRELRDAHIKSDERILGDGDFVNALL
                     ADANEKLERFHIIRHLGYDLDKTARRAAEVCHVALEDIFARSRQKTKVEARSVFCYWA
                     SREAGISYAEIARRLNISLPAVSYSVDRGEKIAKERGYQLLDT"
     assembly_gap    9810..9909
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9924..>11250)
                     /gene="dxs"
                     /locus_tag="PLR47_03375"
     CDS             complement(9924..>11250)
                     /gene="dxs"
                     /locus_tag="PLR47_03375"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOG12009.1"
                     /translation="GALSSYLNRIMTGDRVTRFKSELKSFFKSIPGIGEQILKFSQQI
                     EESVKTFVVPGALFEELGFTYVGPLEGHRLDYLLKNFENVKNLEGPVLVHVITQKGRG
                     YKFAEENSLTYHGIAPFDVETGQTLPSSNPALSYTAIFGRTITQLAADDSRIVAITAA
                     MCEGTGLEKFRAMFPQRFYDVGIAEQLAVTFAAGLAAQGLLPVVAIYSTFLQRAYDQI
                     LHDVCLQNLPVVFAVDRAGIVGEDGATHQGLFDLSYLRNLPHLVMMAPKDENELRHML
                     KTAVGCGSPVSIRYPRGKGVGVPLDGGITTLPLGKGEVLREGSDLAIIAVGCTVHPAL
                     QAAQKLADEGIGAKVINARFIKPLDAELILNTAASIKKILTVEENVLDGGFGSAVLEL
                     LAENGVTGATVKRLGIRNQFVEHAKPSELRSSLGLDEEGILQAAREYGR"
BASE COUNT         2484 a         2922 c         3078 g         2666 t
ORIGIN      
        1 agccactttt tcatgaccat ccataacacg gcttcctttc ttttcttgat ggcggctgtc
       61 attcacgtga ttgtccattg gaaggttctt acgcaatacg tgaacgccag ggtcggagag
      121 tatatgaaat tcaaaagaga gctgttgatt gccgttttgg gcgtgtcggc gtttgtcgtt
      181 ctggtggcgt ttcatgcaat tccttttccg taggttttca gtcggctgcg gatccggcaa
      241 ggcctgcata cggggcattc actgagccgt cgcttaggtc gagcgtgcac cgccgttctg
      301 ccgtgccttt gcggttcttt ggcgcgaaag aagaggaacc gataagatga tacgcgcatc
      361 aacgatgaaa gattttccct acggattgaa attggccgaa gcaatcgagc cgctgttcgg
      421 acccgtgatt gatgatccgg ctttttgcga cggcttgaaa caggtgatcc gtggtcatct
      481 tcagacataa aagaacagtc ttctaaaact taaaattaaa tccggctccc cgtgagcctt
      541 atcattgcgt ccggtggaga aaaattcaga tgaaaacaaa caagattcga ttcctccttg
      601 cggcgatgct ctcctttgtt ttttcaattc tttataatgg gatcgttcat ctggtcattc
      661 ttggcgaagc caaccagcaa gtggaatcat tgaggcgggt ggatttttca tcaaaagtat
      721 ggatatccat cctggcaacc ctcgccgttt cttttctgtt cacggcactc tattcgttct
      781 ttgtttcaga gaagagtatt cgaaaaggat tgtttttcgg ttttgtcttt gggctgttaa
      841 tcgccgtcat cgtggacatc aatcagtatg ttctctatcc gttgccgttc ttgctggttg
      901 caaaatgggc atggtttgga atgatggagt tcagtctgat gggcatgatc gtgggttcta
      961 tcgtcaaaga caagacctaa ccttcgtatc ccgacgggtg ctggttgccg cctgcgcccg
     1021 cgcaaccgct tcgttcgaga ttgagctaag gctcggagtg tggcttggtt tgcaggcaat
     1081 ctttcagggt caggacaaat tgaaagcacg gaaaattcga tccggcaaga tccggccttt
     1141 taaagaaaat tcttttctga tagtctttcc gcgagatgca attcaccacg aatcaggaat
     1201 ggaagatgaa tctgaccgtt tcctccatga attccgaaga agtcgccctg gcggtggatt
     1261 gggccgccag agagggatgg aatccgggcc ttcatgacgc agcctgtttt tatgctgccg
     1321 atccgacggg attttttctg gcccggttcg gcggaaaacc ggtgggatgc atttctgccg
     1381 tagcttacga cgagcgattt gcgtttgccg gcttctacat cgtcgaaacc gaataccgta
     1441 cacagggcat cggacatgcg ctggttgaaa aagcgcagga tcatgtcggc ggccgcatca
     1501 tcggaaatga tgccgtcgtt gcccagcagg aaacttaccg gcgtctggga tttgccgtcg
     1561 cccaccgcaa tatacgtttt cagggcaagg cgactcgcgc tctcctgcgt ccatccgaaa
     1621 tcgttgatct gtccgttgtg ccgttggatg accttttggc ctatgatcga aagatgtttc
     1681 ctgccccgcg cacgtctttt ttgcggcagt ggatccggca gcccgaaggc gcggctctgg
     1741 gccatgtaaa aaaaggaagg ctttccggat acggcgtgat tcggaaatgc cgccgggggt
     1801 ataaaatcgg tcctttgttt gccgatgatg aagaaactgc agggatttta ttcggggcgc
     1861 ttacagaaac cgccgccggt cactccttct gcctcgacat tcctgaaccc aacgcaggcg
     1921 cacaaaggct ggtgacgcgc catggcatgg aaaaggtttt cgaaaccgcg cgcatgtatc
     1981 gcggtccggc cccgatcctt ccgctggaaa aaattttcgg cgtcacgagc tttgaattag
     2041 gctagcctga cccctggaga acccctatag aagctgcgga aggaaggttg cgattttcgg
     2101 aaagaccatc aacaagaccg ccagcgcaat catcgcgtag agcatattca aagaaccctt
     2161 gaagacaata tgaagcggca cgtcgcgggc cgcgcctgcc accacgccgg tggccagcgc
     2221 cgggtcgtac ttgtattttt tcatctcggt aagcgccatg gcaaatccca cgggcatccg
     2281 ccaaaacatc aaaataatca gtacggcaaa gccgatcaaa ccggtcgtgg tgggagtcat
     2341 gcgtccttat ttcttctggt gattggtctt gcccttggaa ttcggtgacg gcagttcagg
     2401 atcaaccatt ttattttcag aatcatttgt cccatgatcc gacttgtttc cggggcgaac
     2461 cacgatgaaa gcaacagcaa taacaattgc tatggcaaga aatattaata actcataacc
     2521 gagactagtc atggttttta tcctccttaa tgcttgaatg atgtgattgt tccaatgcat
     2581 ttcatgacga tttcttcttt tgattgatgt cttcagtccg ttgaaaagac aatgcttgcc
     2641 agcaacgtga ggcaggtttt gatccgccat tcatgttgaa tgagaacaaa agacaaatct
     2701 gaagggcaat gtcaggaaac gattcttgcg ggaggtgctc gcgtggattt tctcgaaagg
     2761 aaaacatctt ttggacttgg atattgtacg ctaaggtgtg tgtagtcaga aagtcgggcg
     2821 ttgtggaaga tgccgtaatg cttaaaaaca attcccggaa aatggggttt catccggcag
     2881 ttgttttcag cgttgccgtc ggcggaataa ctgccatttt gtatgacgac gactttttcc
     2941 gggccgtaac cgaagttgca aacgccatgg gtgacggctg aagacaataa aacagccagg
     3001 aagagagaaa aggctttagt gttcttgatc gacctgaata ttttcattcc actccaccaa
     3061 tgatgcaaaa tgtctttata tggtttcctt aacgaaagca agcccaacct cagagccatt
     3121 gaacgggaat tttgactcgt cattgacaag ttttgctttg tgttatttaa tgcttccatg
     3181 catgtgcaaa gcgtgacact tcatttttat aaaacttttt tagaaaagcg gtaaaaggtt
     3241 ttgtccatcc ggaagaaagg ggcaacacat gcggccgatt cgtgagattc tggaagcgca
     3301 aagcgtcgtc gtaatcggag cgtcgcggga tcaggaaaaa cccggcgcgc agcttctgaa
     3361 ggtattgatg gaagtgggat ttgcgggccg cgtggccggc gtcaacccgc aggggggaga
     3421 ggtgttcggc cttccattat attcaagcgt gcgggacgtg ccgtttgaca tcgatctggc
     3481 ggttttgctc attccgcccc gttttgttcc ggacgctttg cgcgactgcg ccgccagggg
     3541 cgtcaagggg gcggtgatct ccgccgaagg tttcgcggaa acgggaacgg aaggcgccgc
     3601 ctatcaggag gatgtccgcg ccattctgaa atcctccggc atgagaggct tcggccccaa
     3661 caccctggga ctggtcaaca ccaaaaccaa actgaccact tcttatttcg ccaattcccg
     3721 aatgcttgcg cccggcagca tcggttttgc cgcgcagtcc ggcatttttg tgggcgcgct
     3781 gttgcgctac ctgagttcgc tcgacggcct tcatctgtcc aaaggcatcg gcctgggaaa
     3841 caaggtggac gtcaacgaat ccgatgccct ctccttcatg gccgacgatg aacagacgaa
     3901 aatcatcggt ctgtatctgg aagacgtgaa ggacgggaac cggtttgcga aaacgctgaa
     3961 gaaggcggcg gccaaaaagc cggtgctgat tatcaaaagc ggccgcacgc gggccggcgc
     4021 gcaagcgtcc gcgtcgcaca ccgcgagcat ggcggtggaa gacgcagtat ttgacggaat
     4081 cgtgcggcag gcgggcgcga tccgccttca cgccattgac gagtttgtcc ggacgctgaa
     4141 aggttttttg aacatgccgc ttcccttagg cgaccgcctg gcgtatgtct cttacagcgg
     4201 cgcgcaggcg atcatgagca tcgacacgac gatggaacag ggattgcaga cagcgaagct
     4261 cgcggatgcc acactggcca ggatcggccg ggtgatcgca acctcctcca agacaaaaaa
     4321 tcccgttgac atctttcccg actggctgac ccacggttac gagaaaacca tcacggaagt
     4381 ggtgaaagcc cttctggatg accggggcgt tgatggaatt gtttttattt cctttgccga
     4441 aacgaatccc gacgtctatc agtccctgat cgatgtcgtt caatcggacc gccgcaagcc
     4501 cgtctttgtg tcgcttctgg gagacagccg ggataaagcg gcgtgcgagc atctgttaga
     4561 agcaggcggc cttccctgct gtgattttcc ggagcaggcc gtccgtgtct tcgcccgcat
     4621 gcgccaatac gccggaaaaa cgggtaaccc caattcccgc gggtcttctt agaacgggaa
     4681 gaaaacggga atcaggagcg tcgcgagaat ccagaagatg atgttgagcg gcgttcccac
     4741 ctttataaag tctgaaaact tataccggcc gacgccgtaa atcatggtgt tggtctgata
     4801 gccgaccggc gtcatgaagc tcgcggaggc ggcaaaggca attgccatta aaaacggcgt
     4861 cgggcttacc cccatggctt tggcggcggc gatcgcgatc ggcgcgagca ggacggccgt
     4921 cgcgttattg gacatcattt ccgtcatgag cgtcgtcgtc aggtacaggg cggcgacaat
     4981 ggccaccggc cccaagccgc ccagaaaggc aatcattttt ccggacagat acatggccgc
     5041 acctgtctta tccagcgcca cgccgagcga aatcacgccg cccagaagaa aaatgacctt
     5101 ccagtcaatg gcatcgtagg cttcttccag cgtgatgcag cctgccacca ccagcagaat
     5161 cgcgccgacg atcgcgccga ccatgatcgg cacgatgccc agcgcggcgg tgacgatgac
     5221 ggcgagaatg ataagcaccg cgggaaggat cttgcttttg cggtactgcg gggtctcaat
     5281 gtccgacacg atgacgaagt cgttgttgct cttgagggct tcatagttct cccggcgggt
     5341 ttccactaaa atggcgtcgc cggctttgag cggtgtgtcg gcaaagccgg tgttgaaaag
     5401 ctgtccgttg tggcgcagcg ccagcgcggt ggcgcggaaa atattccgga aggcggagga
     5461 cttgatggtt ttgccgatca ggcgggaatt cggcgcgacg accacttccg tcaaacgcag
     5521 ttcttcgcag cggaagtcat tgtcgtgcag ttcgcagtcc gacttcatcg taatgcccat
     5581 gcgctccttg agcttctgaa gctgccggat atcgcagcgc acccggagca gatcgccggc
     5641 cgcgaggaca atgtcggaca cgggcacgag aagccggtgg ccgtggcgga tcacttcgag
     5701 gatgtcgatg tccagctcct tcaccagcgg ggaatccgcc acccggtggc cgaccgattt
     5761 ggccttgtcc tgcaaaacaa tgtccgtgag gtagttctgc atccggtatt tgtccgtcag
     5821 atcgtcctcc gccggatgcg gcggaatcag ccgcacgccg accagcgcca tgtaaagcgt
     5881 gccggccgca aaaaagacaa ggcccagctt ggtgaattca aacatgccca gggcgggcag
     5941 accgtgctgg acggcaatcg aactgactaa aatgttcgtg gacgtgccga tgagcgtgca
     6001 gacgccgccg aacatggaag cgaacgacat cggcatcaaa agcctgctgg ggctgagttt
     6061 gttggtcgcg gccagcccca gcatgatggg aatgaatacg gcgaccaccg gcgtgttgtt
     6121 gacgaaggcc gacacgacgc cgaccagaac catggtgccg acaaggccca cccagaaatt
     6181 gaaccggaag attttcgacg acagagtgcc caaaaaggtt actgcgccgg ttttctgcag
     6241 ggccgcgctc agaatgaaca tggcgccgac cgtcaccgtg gcgacgttgc tgaatccgga
     6301 aaggccctcc gtgggcgtca gaatgccgct gatgatcagg accgccatca ccaggatgga
     6361 ggtcagatcg acgggaagtt tctcggaggc gaaaagaaaa atcgccagca gaatgacgcc
     6421 cagcacgata agcatttctg aagtcatagg gattgcctgc cagcctcacg ggaatgaatt
     6481 tattttacat ctgctacagc actttgagat gttagcatgg tttaaaaggg gcggcaacag
     6541 gaaagcttta tagaacaata gcatgacaca aacccatgtt ccctataccg cactgccgtc
     6601 ggctcaccgt caatggatca aggctctgga tgttttatcc gaaacgcaaa acgatgacgg
     6661 atcctggggg agacaagacg gggaatggaa tacctttctt gctgtccgcg cgctgaaaaa
     6721 catggtctgc ctgtgatgcg gcgaaaaaag gggcgcgggg aagattcccc tgcgcccctt
     6781 ttcggatttg gtcaggatcg aacgattttg ccgatcgatc aggcgctgta aagcgcgatc
     6841 agccgatcac cgtatgtttt catgatgttg cgccggataa gcttcatcgt ctgggtcatc
     6901 atgccgttat cgaccgtgaa atcctcggtg atataaagga acttctcggg aatctcgtac
     6961 ccgccgaacg ttttgcgaag atgatctttg atttccttgg agatgaaatc cgtgcattcc
     7021 ttgttggcga tggtttcctc gcgggagccc tgcgcccatg cggacgtttt gggatcggtc
     7081 ttcagcgcct caaagtccgg aacgaccagc gcgacgttgt ataagcgccc gtccccgtaa
     7141 acgatggcat tggagacata ccgcaggagc ttcatttcgt tttcaatgga ttcgggatgc
     7201 acgtatttgc cgttttccag tttgtattcg tccttgaacc ggccggtgat gtgcagatag
     7261 ccgtcttcat cgagccagcc gcggtctccg gttcgaacac cggggaagcc cttccaggta
     7321 tccggcatca tggcttccct ggtgatggcc ggtttgttgt gatagccctg catgacctgc
     7381 gcgccgtaca ccacgatttc gccgtccggg ctgtcttccc ccacgcgtga tttgtcgatg
     7441 accacattgg tatccttgaa aggcttgccg accgttccgt acttgttgcc ccgtttgggc
     7501 gaattgttgg aaatgaccgg cgtggtttcc gaaagtccgt agccgtcgta ggtgggcacg
     7561 ccgacatcgg cgaagaacag ggcgatttcc gaatcgtgga aaaggccacc gcgtcgtaga
     7621 atttgaattc ttccgttttt tctttcagat cccggttttt gaccgcttcg gctaccgccg
     7681 cgtcaaaaac tttctttttt gccggatcag cggcgacacc ctgctgaatt ttatcgtaaa
     7741 taatattgaa gacgcgcggc acggcggaca tgtgagtggg tttgacttcg ctgaaattga
     7801 cgaggagctt atcggcggat tcggcaaagg caagagccca gccgcccgtg atgtaagtat
     7861 gcaaatccgc ggtcaggccg aaactgtgag cccaggggag aatgcacaaa cccacttcct
     7921 ccgggtctcc ctcatcgaaa gtaacatggc actcgcgggc gttgtgcgtc atgttgccgt
     7981 ggtgcagcag tacgcctttc gggtcgccgg tggtgccaga ggtatagatg ataaaggctg
     8041 tctccgacca gtgcggtttg taggaggggg cggggttttc cttgcccatc ttctccagcg
     8101 ccgccagaga tttttcccct tcgccgaata taataaagac atcctttaag gtcggaatat
     8161 ccttcttgaa gctcatgacg acgtcgtaaa ctttctggtt gctgacaaaa aggtatttaa
     8221 tcgtggcgtc cgaaatgatg tactgccaga ccttctgcag ctctttttcg tacatgggca
     8281 caaaaatgcc gcccagaccg tgggtggcat tggcgcatac aaaccattcc gtgcaattgc
     8341 tgacgatgac gccgactttt tcacctttgc tcaatcccag cttatgaaga gcgccgcgca
     8401 ggttgttcac ccgttcagcg acgtcgctgt agttcaccca ttcgtattga ttggtggtct
     8461 tgttcttggt gccgaacagt ttgtggttgg cgtagcggac cgcgcttcct tcaaaaagat
     8521 cgaccagatt gttcggtttg atgtaatacg cgtccagatt cgaagacgca ccctttcctg
     8581 ctgtcgcttc taccattgct gtacctcctt gtttttcttg atgaaccgat atttttcggc
     8641 aagtcctggc aggcctgacc gtttacattt ccgctgttta tagtcacaac aacccgcacg
     8701 gtgtcaagtt ttaatatagg ggacaccctt aagtatttaa gtatctagga gttgatatcc
     8761 ccgttctttg gcaatctttt ctccacgatc aacagagtag ctaaccgccg gaagactgat
     8821 attcagccga cgggcgatct cggcataaga aatcccggcc tctcgcgacg cccagtaaca
     8881 aaaaacactc cttgcctcga cttttgtttt ttgccggctt ctggcaaaaa tatcttccag
     8941 ggcaacatga cagacctctg ccgcccgtct ggccgtttta tcgagatcgt aacctaaatg
     9001 tcttatgata tgaaaacgtt ctaatttttc gtttgcatca gcaagcagcg catttacaaa
     9061 atcaccatcg ccaagaatcc gctcatcgct cttgatgtgc gcatctcgca actctctttt
     9121 gttgatcggc gaccaccccc caaggctgcg aataagccct cctccgatca agtcggtgcg
     9181 acgcccctgc tcaatgccag ccgcgacaaa ctcattgtag cgtctgattg cgctggcacg
     9241 atttttccca aaatagctca gtacataatc cttttcctgc cagtttcgct ttttatgacc
     9301 cataagaacg ctatggccgc aatagggata gtgattcaat tcctctatgc ttttcaccaa
     9361 tcctgacctg agcggattca gatgaatgta tcgcaccagt tcagtcaaat aggtgtcttc
     9421 ctggcagagg atggatttat agcgattttg aaaaagccgg ccgctacgct tatgacgggc
     9481 attaaaatag actgcgtagc cggtcaggag tctgctcatg agggtggcga tggggacggt
     9541 tcccgaccga aaaagaaaat gggcatgatt ggaaatcagc gcccaggcaa agcaggaggt
     9601 ttgggttgct ggcagaagtt tctccaatcg agccagaaaa ttttcccggt cgtcgttgtt
     9661 tcggaatatc tgtttgcgct ccattcccct gatcataaca tgatgaaggg cgcccggcgc
     9721 gtctattcgt cccgatcttg gcatgagttg tcttttagca aaatcagtct gagccgtcaa
     9781 cttaattact taagggtgtc ccccctaccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnt aagggtgtcc cccctacctc ccgtactccc gggccgcctg aaggatgcct
     9961 tcttcatcaa ggccaagcga agagcgcaat tcggagggtt tggcgtgttc gacaaattga
    10021 ttgcgaatgc ccagtctttt gacggttgcc cccgtcacgc cgttttcggc cagcaattcc
    10081 aggacggcgc ttccgaatcc gccgtccaga acgttttctt cgacggtcag gattttcttg
    10141 atggacgcgg ccgtgttcaa aatcagctcc gcgtcgagcg gcttgatgaa ccgggcgttg
    10201 atgactttgg cgccgatgcc ttcatccgcc agcttttgcg ccgcttgcag ggccggatga
    10261 acggtgcagc cgacggcgat gatcgccagg tcagagcctt cacggagcac ttcgcctttc
    10321 cccaggggca gcgtcgtaat cccgccgtcg aggggaacgc ccacgccttt gccccgcggg
    10381 tagcggatgg aaacgggcga gccgcagccg acggctgttt tgagcatgtg ccgcagttca
    10441 ttttcgtctt tcggggccat catgaccagg tgggggagat tgcgcagata ggacagatca
    10501 aaaagcccct ggtgggttgc gccgtcttcc ccgacaatgc ccgcgcggtc cacggcaaaa
    10561 acgaccggca ggttttgcag gcagacatcg tgcaggatct gatcgtaggc gcgctgcagg
    10621 aaggtggagt aaatcgccac gacgggcagc agaccctggg cggccaggcc cgcggcaaaa
    10681 gtcacggcca gttgttcggc gatgcccacg tcataaaagc gctgcggaaa catggcccgg
    10741 aatttttcca gccccgtgcc ctcgcacatg gccgccgtga tggcgacgat tcgggaatca
    10801 tccgcggcca attgagtaat cgtcctgccg aaaattgccg tgtaggaaag tgcgggattg
    10861 gacgaaggaa gggtctgtcc ggtttccacg tcaaagggcg cgatgccgtg gtaggtgaga
    10921 gaattttctt ccgcgaattt gtagccccgg cccttttgcg taatgacgtg caccagcacg
    10981 gggccttcca gattcttgac gttttcaaag tttttgagga ggtagtccag acggtgcccc
    11041 tccagggggc cgacatacgt gaaccccagc tcctcaaaca gggcgccggg aaccacgaag
    11101 gtttttaccg attcttcgat ctgctgcgag aattttaaaa tctgctcgcc gatgccggga
    11161 atgcttttga agaaactctt cagctcgctt ttgaagcggg tgacccggtc gccggtcatg
    11221 attctgttca aataggacga gagagcgccg
//
LOCUS       DAOSIM010000044        11244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_5726_length_11244_cov_7.494504, whole genome shotgun sequence.
ACCESSION   DAOSIM010000044 DAOSIM010000000
VERSION     DAOSIM010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11244
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5726_length_11244_cov_7.494504"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="PLR47_03380"
     CDS             <1..922
                     /locus_tag="PLR47_03380"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG12010.1"
                     /translation="NTTDDYHLHGLTSLGWELTLCNALFPKNSPCRGALVREASFGEH
                     LFHFLNEKIHLTHLKTVLEIGGGMGYLMKDLLTLAPRLHATMADISPVLLQKQKGSLA
                     GFSAVFLETDFFKIPLTDLRAFDLVILNEVLGDFPTLRAVDGPSSPEAARFAARIADF
                     EADYNLRFDPAENINIGALEALERLCRAGVPCIYLSEHSCESSHSDPAFPGMNFSPSG
                     NPEKISLKGHAEFTVKFSALEKMARAFGYKVFRGPYTDILPISFNDRVTAALRTPSPL
                     SDRQEILRQFLYDLYKYEYLLLASAPAGKG"
     gene            926..1246
                     /locus_tag="PLR47_03385"
     CDS             926..1246
                     /locus_tag="PLR47_03385"
                     /inference="COORDINATES: protein motif:HMM:NF022459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HOG12011.1"
                     /translation="MKKLRIIGLAVILLCANAVPAAAFRCGSGLVSTGDKKVKVQGTC
                     GLPTTKEIICPRKSSVCPATLEIWYYNCGDNDYIYALTFEDGVLTKETTEGGGVGNSY
                     CLGR"
     gene            1272..1625
                     /locus_tag="PLR47_03390"
     CDS             1272..1625
                     /locus_tag="PLR47_03390"
                     /inference="COORDINATES: protein motif:HMM:NF022459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HOG12012.1"
                     /translation="MIRIQTGFIALLILCLAAADASAFRCGSGMVSAGDTKARVLITC
                     GKPTAKERVSEKNKTSATAVKNKTAKKSGSKSEVWYYNCGSQDYIYALTFEDGVLKKE
                     DTEGKGSGKSDCMGK"
     gene            complement(1659..3728)
                     /locus_tag="PLR47_03395"
     CDS             complement(1659..3728)
                     /locus_tag="PLR47_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOG12013.1"
                     /translation="MKDNHLKNEKSPYLLQHAQNPVDWHPWGDEAFTRARLEDKPIFL
                     SIGYSTCHWCHVMERESFEDEDVARMLNETFVNVKVDREERPDIDAVYMEVCKLLTQS
                     GGWPLTIMMTPDRKPFFAATYLPPRSKYGRAGLTDIVGRVGVLWRTRRAELLSSAEEI
                     VNALQKPSAGSARDAGEEELHRAYRLFSMSFDAAFGGFGQAPKFPSPHQLFFLLRYWK
                     RTGEAQALRMAEWTVRNMRRGGIFDQIGFGFHRYSTDERWIVPHFEKMLYDQALMALA
                     CAELYQATGSGEYAAAARETAAYVLRDLTDAGGGFYCAEDADSEAVEGKFYLWTEKEI
                     RAVLDAEEADFFLTHYRHASDRSVPGMREIPEGHFIAHAKPLTDEPGPGQTAFWPVLT
                     EAIREKLFAVREKRVRPHRDDKILTDWNGLMIAALARLAQVLKEPAYAAAAVRAMDFI
                     LRSLRTPDGRLLHRFRGGHSAFAANLDDYAFIIWALLELYEATFDPAYLTRALELHSH
                     LRGFFRDEKDGGFFFTPKDAEPLPVRPKELYDGAIPSGNSVAFLNTLRLSRITGDDAL
                     EKEAREIAGAFGGPAGASPTAFAHFLCGLDFAVGPASEVILAGKEGAPDAQALLGALR
                     RSFTPNKVVIFRPEDTGGADIEAISPRVRSRVCIGGRATAYVCSGFACRLPATDPEQM
                     MALLNGK"
     gene            4181..4453
                     /locus_tag="PLR47_03400"
     CDS             4181..4453
                     /locus_tag="PLR47_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOG12014.1"
                     /translation="MTKAELIGVMADEAEISQAAAAKALDAYIAAVGKELKKTGKLGL
                     VGFGTFSVVKRKAREGRNPQTGKTIKIPAKKVVKFKAGKTLSDKVK"
     gene            4555..5547
                     /gene="trpS"
                     /locus_tag="PLR47_03405"
     CDS             4555..5547
                     /gene="trpS"
                     /locus_tag="PLR47_03405"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOG12015.1"
                     /translation="MTRKRILSGMRPTGRLHLGNLHGALGNWVELQNSGNYDCFYFVA
                     DWHAITSEYASTQDIRQNSLYMVMDWLAAGLDPAKSTLFVQSAVKEHAELFLLLSMIT
                     PLAWLTRNPTYKEMQTELSSKDLSTFGFLGYPVLQAADIVMYKAYGVPVGVDQLPHIE
                     LTREIARRFNYLYQKDVFPVPEPLLTQVPKLLGIDGRKMSKSYDNAVYLSDRGKTLHR
                     KIASMFTDPQRMRKKDPGDPDICNVFTFHGLYSPAPDVAEINRECRTAGIGCTDCKKM
                     LAARVEEALAPVHERMDYYQRNIAEIEEMIQKGNDEAAAIARRTMAEVREAVKI"
     gene            5556..6302
                     /locus_tag="PLR47_03410"
     CDS             5556..6302
                     /locus_tag="PLR47_03410"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOG12016.1"
                     /translation="MNEETSTDYAIKLDIFEGPLDLLLYLIKKNEIDIYNIPIALVTR
                     QYLDYLNLIKSLNLDLAGEYLVMASTLIHIKSRMLLPVPEEPSPEEMEDDPRAELVRQ
                     LLEHQAFKEAASLLTQRPILERDVFKRSAAIVGNERPGAADDEELVEVSIFELIEAFH
                     QIVSRMDKKDLMEIDLEKLSLSDIINDVMERLTREKNLTFDELLGEKRDRRRIVYTFL
                     ALLELIKLKMIKAYQTAAFGVIRIFPAVEL"
     gene            6303..7028
                     /gene="scpB"
                     /locus_tag="PLR47_03415"
     CDS             6303..7028
                     /gene="scpB"
                     /locus_tag="PLR47_03415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOG12017.1"
                     /translation="MENLSAVLEALILSSESPLPLEKICAALGSGDKEEIKSALDRLI
                     TEYDERESGIGIQEVAGGFQFRTRPEMASWIKKLKGVKPASLSPAAMETLAIVAYRQP
                     IVKSEIESIRGVDVSAPLKGLLDKKLVRILGRKDVPGKPIIYGTTKKFLEVFNLRELS
                     DLPTMRELRELTEPQESPEQEAISFEDLLPETPAGEMPKAPETPSGPEAKQAEKTPEE
                     DPAADAEEQEEHGVEPNPGDDAV"
     gene            7028..7852
                     /locus_tag="PLR47_03420"
     CDS             7028..7852
                     /locus_tag="PLR47_03420"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOG12018.1"
                     /translation="MKERLQKIISAAGIDSRRHAEAMITEGRVSVNGEIVRELGSKAD
                     PQKDVIRIDGKIISIENPGCYIALYKPAGYVTTLSDPQKRPTVVDLVRDVPERVYPVG
                     RLDYDSAGLLLLTNDGAFAQKISHPGFRVPKTYRAKIAGRISLEKLKEISKGILLPDG
                     VFKPENLKQEKVNDKSAWISLTLHEGRNRIIRRGFEAAGFTVTRLVRTSIGNITLSGL
                     SEGSWRPLSDREVRELQAPPQGKKGKNILDNHPKINNSRAVRDNRHHKYSGFKQQS"
     gene            7862..8143
                     /locus_tag="PLR47_03425"
     CDS             7862..8143
                     /locus_tag="PLR47_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOG12019.1"
                     /translation="MNKSGLIEALSKKMNLTEKKAIDVVNLVFKGFNDELKKGGRIEI
                     RGFGSFVVKEYGSYTGRNPKTGTKIKVEPKKLPFFKVGKELKDRVDKNK"
     gene            complement(8242..9150)
                     /locus_tag="PLR47_03430"
     CDS             complement(8242..9150)
                     /locus_tag="PLR47_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HOG12020.1"
                     /translation="MRVSPFLYMLAIVAGLLLPQAAPWASLLVLPALTIALTVALLRF
                     PGGFFKNPRTLLPGALWGCLMNYLVLGNLMILGGIFLIKNENFWIGLVILAAVPSALA
                     ALPWSEKLGADHRLTLTGFAGTYLGALILIPLLAAAFLRYIPVCCEKLIIMAAALIFL
                     PLLLSRVAVDRNWDERFHQRMGTVSDVCFFIVFYAAASANSVLIRQGSLDMLFILALA
                     FVSTALIAAALIAAGRVFKLSRPVVSSLLLLGTMKNCGLAAGIALYAVNDEAAVPALI
                     FSIFIFLNGLWLWLWRKGLERRFYRA"
     gene            complement(9173..10003)
                     /locus_tag="PLR47_03435"
     CDS             complement(9173..10003)
                     /locus_tag="PLR47_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOG12021.1"
                     /translation="MDLLDVFTYGFVQRAMLAGSLIAAVSALLGLFLVLRRFSLIGDG
                     LAHTTFGSVAVVLLVGISPFYVTLAALPLVMISSLAIYRLTQSRKIHADAAIGIVSSL
                     GIAAGIILSSLSGGFNVDLFSYLFGNILTVNQTELLLSVIVFALVAAAVVYCYEDLFA
                     VTFDQELARTMGVQTGRINFVLFLLTAVSAVLAMKVAGIMLVSALLILPALTALQLSV
                     SFRKTMMSAVGFAVGAVLLGIVFAFVLNLPAGAMIVLFNIVFLLLVAGVRKFCAGRKA
                     "
     gene            complement(10003..10740)
                     /locus_tag="PLR47_03440"
     CDS             complement(10003..10740)
                     /locus_tag="PLR47_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOG12022.1"
                     /translation="MPVIRVEDLTYRYNGAEVLSGIHFTVERGVYLAIVGPNGSGKTT
                     LVKNILGILKPQQGRVDLFGTRIEDFNQWEKIGYLPQRLSGLNTHFPGTVTEVVQMGL
                     PKKDPGLLSRTLDAMAIGHLARRLIGELSYGEQQRAMLARALIRRPQLLIFDEPTTAL
                     DPETRGIFYGLTQELSRKEGATVVLVTHDSGVVGQYAQHLLYLDKKLIFSGTFRDFCA
                     SVDMEGFFGPASQHIICHQHDLKGSGR"
     gene            complement(10731..>11244)
                     /locus_tag="PLR47_03445"
     CDS             complement(10731..>11244)
                     /locus_tag="PLR47_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOG12023.1"
                     /translation="WLDAANAQKMIDNIAAAFITRDPKNSETYRKNAEGYKRRLMDLD
                     EKYREGLSACSTRTLLHAGHWAFAYLANRYRLHYRSASNSSADAEPSPQQILSLMEQI
                     RKEKLRYIFYEDFTAPKLAAVMADETGVGLLKLNNGHDVGRKDFQSGETYLTLMEKNL
                     AHLREGLRCR"
BASE COUNT         2686 a         3265 c         3152 g         2141 t
ORIGIN      
        1 aaacaccacg gatgactacc accttcacgg gctgacctcc ctgggctggg agcttacgct
       61 gtgcaacgcg cttttcccca aaaacagtcc ctgccgcggc gcgctggtgc gggaggcatc
      121 cttcggcgaa catctttttc actttctgaa tgagaaaatt cacctgacgc atttaaaaac
      181 cgttctggaa atcggcggcg gcatggggta tctgatgaag gatcttttga ccctggcccc
      241 ccgccttcac gcgaccatgg cggacatctc ccccgttctt ctgcaaaaac aaaagggatc
      301 tctcgcggga ttttccgcgg tctttctcga aacggatttt ttcaaaatcc ctctcacgga
      361 tctgcgcgcc tttgacctcg tgatcctcaa cgaagtcctg ggggatttcc ccacgctgag
      421 agccgtggac ggtccgtcat ccccggaagc cgcccgcttc gcggccagaa tcgcggattt
      481 cgaggcagac tacaacctgc gcttcgatcc cgccgagaac atcaacatcg gggcgctcga
      541 agccctggag cggctttgcc gggccggcgt tccctgcatc tatctgagcg aacacagctg
      601 cgaatcgtct cactccgatc cggcgtttcc cggcatgaat ttttcacctt cgggaaaccc
      661 cgaaaagatt tcactcaaag gccatgcgga attcaccgtc aaattctccg cactggaaaa
      721 aatggcccgg gcctttggct acaaggtgtt tcgcggcccc tacaccgata ttctgccgat
      781 ctcgttcaac gaccgggtga cggccgccct tcggacgccg tctccgctgt cggacaggca
      841 ggaaattctc cggcagtttc tttacgatct gtataaatac gagtatcttc tgctggcatc
      901 cgctccggca gggaaaggat agaatatgaa aaagcttcga atcatcgggc tggccgtaat
      961 cctgctttgc gcaaacgccg ttccggcagc ggcctttcgc tgcgggtccg gcctggtttc
     1021 caccggagac aaaaaggtta aagttcaggg aacctgcggc cttccgacca ccaaggaaat
     1081 catctgtccc aggaaaagca gcgtctgccc cgcgactctg gaaatctggt actacaactg
     1141 cggcgacaac gactacattt acgcgctgac gtttgaagac ggcgttctga cgaaagaaac
     1201 cacggagggc ggcggagtgg gcaattccta ctgcctgggc agataaacat cactcgagag
     1261 ggggaagaaa tatgatacgg attcagaccg gcttcatcgc gctgctcatc ctttgcctgg
     1321 cggccgccga cgcttccgct tttcgctgcg gatccggcat ggtttccgcc ggcgacacca
     1381 aggcgcgggt tttgatcact tgcggaaagc cgaccgccaa ggaaagagtc agcgagaaaa
     1441 acaagacttc cgcaaccgcc gtcaagaaca aaacagcaaa gaaaagcggc agcaagtcgg
     1501 aggtctggta ctacaactgc ggcagccagg actacattta cgccctgacc tttgaagacg
     1561 gcgttctgaa aaaagaggac acggaaggaa aagggagcgg taaatccgac tgtatgggaa
     1621 agtagcggcc ggaagcggaa aggaaccgcg gccgttttct attttccatt gagcaaagcc
     1681 atcatctgtt ccgggtccgt tgccggaaga cggcaggcaa agccggagca gacataggcg
     1741 gtcgcccgtc cgccgatgca gacgcgcgac cgaacccggg gcgagatggc ttcaatgtcc
     1801 gcccctcccg tgtcttccgg ccgaaagatg accactttgt tcggtgtaaa gcttcgccgc
     1861 agcgccccaa gcagagcctg cgcatccggc gcgccctcct tccccgcaag aatcacttcg
     1921 cttgccggac ccacggcaaa atcaaggccg caaagaaagt gcgcaaaggc cgtcggggaa
     1981 gccccggccg gcccgccgaa ggccccggca atctcgcgcg cttctttttc cagcgcgtca
     2041 tccccggtga tccgggacag acgcagcgta ttgagaaaag cgacggaatt tccggacggg
     2101 attgcgccgt cgtaaagctc ctttggacgg accggaagcg gctctgcgtc tttcggagta
     2161 aaaaagaagc cgccgtcctt ttcatcgcgg aaaaaacccc tcaggtgcga atgcaactcc
     2221 agcgccctgg tcagatacgc agggtcaaaa gttgcttcgt aaagctccag aagcgcccag
     2281 atgatgaagg catagtcgtc aaggttcgcg gcaaaggcgc tatgcccgcc gcgaaaacgg
     2341 tgcagcaggc ggccgtcggg ggttctgagc gatcgcagga tgaaatccat ggcgcggacc
     2401 gcggccgctg cataggccgg ttccttcagc acctgcgcca ggcgcgccag cgcggcgatc
     2461 atgaggccgt tccagtcggt gagaatcttg tcgtcccggt gagggcgcac ccgtttttcc
     2521 cgaaccgcga aaagcttttc ccggattgct tccgtcagga ccggccaaaa agcagtctgg
     2581 cccggcccgg gctcatccgt cagcggtttt gcgtgcgcaa tgaaatgccc ttcgggaatt
     2641 tcccgcatgc ccggcacgga ccggtcgctt gcatgacggt agtgcgtaag aaagaagtcc
     2701 gcctcttcgg cgtcaaggac ggcgcgaatc tccttttcgg tccacagata gaactttccc
     2761 tccacagcct cgctgtccgc atcttcggcg cagtagaatc ctcctccggc atccgtcagg
     2821 tcccgcaaaa cataggcggc ggtttcccgc gcggccgccg cgtactcccc gctgcctgtc
     2881 gcctgatata gctcggcaca ggccagcgcc atgagcgcct ggtcgtacag catcttttca
     2941 aaatgcggca cgatccagcg ctcgtccgtc gaatatcggt gaaatccgaa gccgatctga
     3001 tcaaagatgc ctccgcggcg catgttccgc accgtccatt ccgccatgcg cagggcctgc
     3061 gcctcgccgg tccgcttcca gtagcgcagc aggaaaaaga gctgatgcgg cgacggaaat
     3121 ttcggcgcct gcccgaatcc gccgaaggct gcatcaaagc tcatggaaaa caatcggtag
     3181 gcccggtgca gctcttcttc ccccgcgtcc cgcgcgctgc ctgcggaagg tttctgaagg
     3241 gcgtttacaa tctcctcagc cgaggaaagc agctccgcgc gccttgtgcg ccacagaacc
     3301 ccgacccggc cgacgatgtc cgtcaggccg gcccgcccgt atttcgatcg ggggggaagg
     3361 taggtggcgg cgaagaaagg ctttcgatcc ggcgtcatca tgatcgtcag cggccagccg
     3421 ccgctttgcg tcaaaagttt gcagacctcc atataaaccg cgtcaatgtc ggggcgctct
     3481 tcgcggtcca ctttgacgtt cacaaacgtt tcattgagca ttcgggcgac gtcttcatcc
     3541 tcaaacgact cccgttccat gacgtggcac cagtggcagg tggaataacc aatggaaaga
     3601 aatatgggtt tatcttcaag gcgcgcccgg gtgaaagcct catcacccca gggatgccag
     3661 tccaccggat tttgcgcgtg ctgcaggagg taggggcttt tttcgttttt caagtggttg
     3721 tctttcatcg cccttgctcc ccttcggctc gccggcattc gttcatctgc ggcgcgtgtg
     3781 gattcgtagc acaggcggga gtgcttgtca aagaagacgg ctcgggccgg agacggcgtt
     3841 cacgcacggc atgcgcctgt ttcaccgccg tctgcaaagc gccctgccgc ccgcgaaatc
     3901 gcccgtgaaa accggccgca aaaatacttt tttcttgatt gttgtttttt tttgtgttag
     3961 ccctagcgcc ggtttgaggc gtgccgcgct tcgtcaacag tacaaacaac agggttccat
     4021 tcattcccat gaagcattcg gccccggtcc tgaagatcgc gccccttgac cttcatcagg
     4081 gcgtcaagca cgggagcccg gatcccagag gcaaatcaca gccgcgccgg gcggctaatg
     4141 aaaatttaac catataaatt caggaaaggg ggactggagc atgacgaaag cagaattgat
     4201 cggagtaatg gcggacgagg cggaaatatc ccaggcggcg gcggccaaag cgctggatgc
     4261 gtacatcgcc gcggtgggga aagaactgaa aaaaaccggc aagctgggat tggtcggttt
     4321 cggcacgttc tccgtcgtca agagaaaagc aagagagggc aggaatcctc agaccggcaa
     4381 gacgatcaag attcctgcga agaaagtggt taaattcaag gccggcaaga ctttgtcgga
     4441 caaagtgaaa tagggctttt ttaagtcatc cttttaacca aggcctccgt cagcttttcg
     4501 aaacaccgga ggccttggtg catttatcca ttttggaaac gaggagcgtc tggcttgaca
     4561 cgaaaaagga ttctcagcgg gatgcggccc acaggaaggc tgcaccttgg aaatttgcac
     4621 ggcgcgttgg ggaactgggt cgaactgcag aacagcggca actacgactg cttttacttc
     4681 gtcgccgatt ggcatgcgat caccagtgaa tacgcatcga cacaggacat tcggcaaaac
     4741 agcctgtata tggtgatgga ctggctggcc gccggcctcg atccggccaa aagcaccctc
     4801 tttgtgcaat cggcggtcaa ggaacacgcc gaattgttcc tgctgctttc catgatcacg
     4861 ccgctggcct ggctcacgcg caatcccacc tacaaggaaa tgcagacgga attgagctcc
     4921 aaagatcttt ccaccttcgg ctttctgggc taccccgttc tgcaggcggc cgacattgtc
     4981 atgtacaagg cctacggcgt tccggtcggc gtggaccagc ttccccacat tgagctgacc
     5041 cgcgaaatcg cccggcgctt caactacctt taccagaaag acgtctttcc cgttccggag
     5101 ccgcttttga cccaggtgcc gaagctgctg ggcatcgacg gcaggaaaat gagcaaatcc
     5161 tacgataatg ccgtttacct gtccgaccgg ggcaaaacgc ttcaccggaa aatcgcctcc
     5221 atgttcaccg atcctcagcg aatgcggaaa aaagacccgg gcgatcccga catctgcaac
     5281 gtattcacct tccacggcct ttactccccc gctccggacg tggccgaaat caaccgggaa
     5341 tgccgcacag ccggcatcgg ctgcaccgac tgcaagaaaa tgctggcggc gcgcgttgag
     5401 gaagcgctgg cgcccgttca tgagcgcatg gattactatc agcgcaacat cgcggaaatt
     5461 gaggagatga tccaaaaagg caacgacgag gccgcggcga tcgcccgccg gaccatggcg
     5521 gaagttcgcg aagccgtcaa aatctgaagc gacctatgaa cgaagagacc tccacagatt
     5581 atgcgatcaa gctggatatt ttcgaaggtc cgctggacct gctgctgtat ctgatcaaaa
     5641 aaaatgagat tgacatttac aacattccca ttgctctggt cacccgccag tacctggatt
     5701 atctgaacct catcaagtcg ctcaatctgg acctggcggg cgaatatctc gtcatggcct
     5761 ccacgctgat tcacatcaag tcccgcatgc ttctgccggt tccggaagag ccgtcgcccg
     5821 aagaaatgga agacgacccg cgcgcggaac tggtccgcca gctcctggag caccaggcct
     5881 tcaaggaagc cgcctccctg ctgacccaga ggcccatcct ggagcgggac gtgttcaagc
     5941 gctccgcggc gattgtcggc aacgaacgcc ccggggcggc agacgatgag gagctggtgg
     6001 aagtcagcat ttttgaattg atcgaagcct ttcaccagat tgtttcccgc atggacaaaa
     6061 aggatctgat ggaaattgac ctggaaaaac tgtcgctttc ggatatcatc aacgacgtga
     6121 tggagcgcct tacgcgcgaa aaaaatctca cctttgacga attgctgggg gaaaaaagag
     6181 accgccgccg gatcgtttac acttttctgg ccctgctgga gctcatcaag ctgaagatga
     6241 tcaaggccta tcagacggcg gccttcggcg tcattcgaat cttcccggcc gtggagttgt
     6301 aaatggaaaa cctttcagcc gttctcgaag ccctgatcct gtcgtcggag tcgcccctgc
     6361 cgctggagaa aatctgcgcc gcgctcggaa gcggggacaa ggaggaaatc aaaagcgccc
     6421 tggatcgtct gatcacggaa tacgacgagc gcgaaagcgg aatcggcatc caggaagtcg
     6481 cgggcggttt tcagtttcgc acccggccgg aaatggcctc ctggatcaaa aaactcaagg
     6541 gagtcaaacc ggcctcgctg tcgcccgcgg caatggaaac actggccatc gtggcctacc
     6601 ggcagccgat cgtcaagtcg gagattgaaa gcattcgcgg cgttgacgtc agcgctccgc
     6661 tcaagggcct gctggacaaa aagctggtgc gcatcctcgg gcgcaaggac gtgccgggaa
     6721 agccgatcat ttacggaacg accaaaaaat ttctggaagt tttcaatctc agggagcttt
     6781 cggacctgcc gacgatgaga gagcttcggg agcttaccga acctcaggaa tcgcccgaac
     6841 aggaagccat ttcctttgaa gaccttctgc cggagacgcc tgcaggggaa atgccaaagg
     6901 ctcctgagac gccttcgggt ccggaagcaa agcaggcgga aaaaaccccc gaagaggacc
     6961 ccgcagccga cgcggaagaa caggaagaac atggggttga gccaaacccc ggggacgatg
     7021 ccgtttgatg aaagaacgcc ttcagaaaat catctccgcc gcgggcatcg attcccgccg
     7081 tcatgccgaa gccatgatca cggagggccg ggtgagcgtc aacggagaga tcgtgcgcga
     7141 actgggctct aaagccgatc cgcaaaaaga cgtgatccgc attgacggca aaatcatctc
     7201 cattgaaaat ccgggctgct acattgcgtt gtacaaaccg gcgggctacg tcaccacgct
     7261 gtccgatccg cagaaaagac cgacggttgt cgatctggtc cgggatgttc cggaaagggt
     7321 atatccggta ggccggctcg attatgattc cgcgggcctg ctgcttctga ccaatgacgg
     7381 agcgtttgct caaaaaattt cccaccctgg cttccgggtc cccaaaacct accgggcaaa
     7441 gattgccggt cgcataagcc ttgagaaatt aaaggaaatc agcaagggga ttttgctgcc
     7501 ggacggtgtt ttcaaaccgg aaaatctgaa gcaggaaaag gtcaacgaca aaagcgcctg
     7561 gatcagcctg accctgcacg agggcagaaa ccggatcatc cgcagagggt ttgaggctgc
     7621 cggtttcacg gttacgcgcc tggtgcgcac ctccatcgga aacataacgc tttcgggcct
     7681 gtcggaaggc tcatggcgcc cgctttcgga ccgggaagtc cgcgagctgc aggcgccgcc
     7741 gcaggggaaa aaaggtaaaa atattcttga caatcatcca aaaataaata atagtcgcgc
     7801 cgtacgggat aatcggcatc ataaatactc gggtttcaag cagcagtctt aaggaggatc
     7861 tatgaataaa tccggtctta ttgaagcttt aagcaagaag atgaatctga cggagaaaaa
     7921 agccattgac gtggtcaatc tggttttcaa aggcttcaac gatgagttga aaaaaggagg
     7981 ccgcatcgaa attcgcggat tcggcagctt tgtggtgaaa gaatacggat cctacacggg
     8041 aagaaatccc aaaaccggaa caaaaatcaa ggttgaacca aaaaaactgc cctttttcaa
     8101 agtgggcaaa gagctgaagg acagggtcga caaaaacaaa tagaaatcct gcaagtgttt
     8161 gttatactta atgaagggga cttggaaaca agcccccttc attttggttt gccaaatgat
     8221 gagctctcct tgcgtttcag ctcaggcccg ataaaatctc ctttccaggc ctttgcgcca
     8281 aagccacagc cacaatccgt tcagaaatat aaaaatggaa aaaatcaaag ccggaacggc
     8341 agcttcgtcg ttcacggcat acagggcgat gccggcggca aggccgcaat ttttcatggt
     8401 tcccagaagc agaagcgatg aaaccacggg gcgggacagt ttgaaaaccc tgccggccgc
     8461 gatgagcgcg gcggcgatca gggccgtgga aacaaaagcc agcgccagaa tgaaaagcat
     8521 gtccaggctc ccctgccgga tcaggacgga attggcggaa gcggcggcgt aaaacacgat
     8581 aaaaaagcag acatcagaaa cggtccccat gcgctggtga aaccgttcat cccagttgcg
     8641 gtcaaccgcg accctggaca gcaacagcgg aaggaatatc agcgcggcgg ccatgatgat
     8701 cagcttttcg cagcacacgg ggatgtaccg gagaaaagcc gccgcgagaa gcggaatcag
     8761 gatgagcgcg cccaggtagg tcccggcaaa accggtcagc gtcagcctgt gatccgcgcc
     8821 gagcttttcg ctccagggaa gagccgccag agcggaagga acggcggcca ggataaccag
     8881 gccgatccag aaattctcat tcttgatcag gaaaattccg cccagaatca tcaggttccc
     8941 cagaaccagg tagttcatca ggcagcccca gagagccccg ggaagcagcg tccgcggatt
     9001 tttgaagaat ccccccggaa agcgaagcag cgcaaccgtg agggcaatcg tcaacgccgg
     9061 cagaacgaga agcgaagccc agggggccgc ctgaggcagc aaaaggcccg ccacaatggc
     9121 aagcatgtaa agaaaagggg ataccctcat ggccgtcctc atcggagcgg gatcatgcct
     9181 tgcgcccggc gcaaaatttc cggacaccgg caaccagcag aagaaaaacg atattgaaca
     9241 ggacaatcat ggcgcctgcc ggcaaattca ggacaaaggc aaacacaatt cccaggagca
     9301 ccgccccgac cgcaaacccc acggcggaca tcatcgtctt tcggaaactg accgagagct
     9361 gcagcgcggt gagagcgggc agaatcaaga gcgccgaaac cagcatgatc ccggcaactt
     9421 tcatggccag aaccgcggat acggccgtca gaagaaaaag aacgaagttg atcctgccgg
     9481 tttgaacgcc catggtgcgg gcaagctcct ggtcaaaggt caccgcgaac agatcttcgt
     9541 agcaataaac aaccgccgcc gcgacaaggg caaagacgat cacggaaagc aacagttcgg
     9601 tctggttcac ggtcagaata tttccaaaaa gataactgaa caaatccacg ttgaagccgc
     9661 ccgaaaggct ggacagaatg atgcccgccg cgattcccag ggaagaaacg atgccgatgg
     9721 cggcatcggc atgaatcttt ctcgattggg tcagccggta gatggcgagc gaggaaatca
     9781 tcaccagcgg cagagccgcc agcgtaacgt aaaacgggct gatgccgacg agcagcacga
     9841 ccgccacgct gccgaaggtg gtatgcgcca ggccgtcccc gatcagggag aagcgcctca
     9901 gaacgagaaa caggcccagc agcgccgaaa ccgcggcaat cagggaaccg gcaagcatcg
     9961 cccgctgaac gaatccatac gtgaaaacat cgagcagatc catcagcggc cgctcccctt
    10021 caaatcatgc tggtggcaga tgatgtgctg actggccggg ccgaaaaatc cctccatgtc
    10081 caccgaagcg cagaagtccc ggaatgttcc ggaaaagatc aatttcttgt ccagatacag
    10141 cagatgctga gcgtattgac cgaccacgcc gctgtcatgg gtcaccagca ccaccgttgc
    10201 gccttctttc cggctcagct cctgggtcag cccgtagaaa atgccccggg tttcggggtc
    10261 cagcgcggtg gtcggttcgt caaagatcag caactgcggc cgccggatca gcgcccgcgc
    10321 cagcatggcc cgctgctgtt cgccatagga cagctcgccg atcaaacgcc gggccaggtg
    10381 gccgatcgcc atcgcgtcca gtgtgcgcga cagcagaccc gggtcttttt tcggcaggcc
    10441 catctgcacg acttccgtca ccgtgccggg aaagtgtgta ttcaaaccgc tgagccgctg
    10501 gggcagatag ccgatttttt cccattggtt gaaatcctcg atccttgtgc cgaacagatc
    10561 cacccttccc tgctgcggct tcaggatccc cagaatattt ttgacgagcg tggttttccc
    10621 cgatccgttg ggacccacga tggccagata gacgcccctt tccacggtaa aatggatccc
    10681 gctcagcact tccgccccgt tgtagcggta ggtcaaatct tctacgcgaa ttaccggcat
    10741 cgcaaacctt ctctgagatg agccagattt ttttccataa gcgtcagata cgtttcgccg
    10801 gactgaaagt ctttcctgcc gacatcgtgt ccgttgttga gcttgagcag acccacgccc
    10861 gtttcgtcag ccatcaccgc cgcgagcttc ggcgccgtga agtcttcgta aaaaatatag
    10921 cgcagcttct ctttcctgat ctgttccatc agcgacaaga tctgctgagg ggaaggctcc
    10981 gcgtccgcgg aactgttgga cgccgaccgg tagtgaagcc ggtaccggtt ggccagatac
    11041 gcgaaagccc agtgccccgc atgcaaaagg gttctggtgc tgcacgcgga aagcccctcg
    11101 cgatattttt catccaggtc catcagccgg cgtttgtagc cttcggcatt tttccgatag
    11161 gtctcgctgt ttttcgggtc ccgggtgatg aaagccgccg cgatgttgtc gatcattttc
    11221 tgcgcgttgg cggcgtccag ccag
//
LOCUS       DAOSIM010000045        11040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_5850_length_11040_cov_6.965498, whole genome shotgun sequence.
ACCESSION   DAOSIM010000045 DAOSIM010000000
VERSION     DAOSIM010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 11040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11040
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5850_length_11040_cov_6.965498"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLR47_03450"
     CDS             complement(<1..312)
                     /locus_tag="PLR47_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12024.1"
                     /translation="MADAGNPFPFNESSVFPWLKTAADMWLNMAKTIPPDSDTTLKTQ
                     TDVQNRFTRQLETSLNLFKSFSRMMNEPESASATANTMNALPEIFLKMAGSGFEAAMQ
                     IQ"
     gene            complement(408..908)
                     /locus_tag="PLR47_03455"
     CDS             complement(408..908)
                     /locus_tag="PLR47_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HOG12025.1"
                     /translation="MKNTLVFIHGLESTSQGNKAQYFRKAFPEMIIEDYTGGFGTRMQ
                     KLQSVLAGKTNLILVGSSYGGLMAARFALDNEPLIQKLILLAPALTIEGFEQAVRQKL
                     QIPTVLYHGTRDDVVPSQAVKAIAEKTFARLEHHFVDDDHPLNAVFPTLPWPLLLEQG
                     PAAETD"
     gene            1011..2240
                     /locus_tag="PLR47_03460"
     CDS             1011..2240
                     /locus_tag="PLR47_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOG12026.1"
                     /translation="MAQVLPFKAVRPQTKYASQVAALPYDVMTREEGQKATAGRPLSF
                     MHVEKSEIDVPDGTRPDDPLIYETAKRNFARLREEGILIQDAAPCYYVYRQKMGPQVQ
                     TGIVGVMSAAEYDEGKIRKHELTRQDKEDDRIRHVDQVNAQTGPVFISYKTRPGLNEI
                     VEKITAGRPEYDFTAEDGVVHTAWVVADQAQVDAIRSQFDQVAALYIADGHHRAAAGA
                     TVARRRRKPSQTQESDSVLAVFFPHDQLKVMDYNRAVKDLRGLSAEAFLEKISSSFTV
                     TKGFAEKSPRRLHDFGMYLGGAWHCLTIKEGAYDANDPVASLDAAILQDHLLAPVLGI
                     ADPRTDDRIKFIGGIRGMAELEKLVDQDGFAVAFSLYATTMEQIMNVADAGAIMPPKS
                     TWFEPKLRSGLFVHSLE"
     gene            complement(2384..3436)
                     /locus_tag="PLR47_03465"
     CDS             complement(2384..3436)
                     /locus_tag="PLR47_03465"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG12027.1"
                     /translation="MYYLSSDEQAVWEEHARQAMDGKLTPRSSLDGIRNALRAHFHFY
                     MGGLLAGLGKRPEAADWFREGALEEDGIFNAIMASYLERHNGRFYMPEKIFADPRDYE
                     HFTTVPAIVGMREKFLRFASSSLPRFADPLTVLDVGCGDGSLLVLLLQRLMKTGRAQN
                     IARAILVDSSASMMEVAEGKLRKAFPELTVDPRHGRIQEHAGELPAGIDIALLSLAYH
                     HMPCDAKAAHLREMSGKVNHLLLMEMDGDNDTPQLGSPELAVSVYQSYGPLIDSIFAH
                     DAPITVTQSCVDNFVMAEVISFLTEPRGRRNDYHMLRGQWERVFRENLDGHHPRGEAT
                     VLASDGCEFFACHWGR"
     gene            3552..4202
                     /locus_tag="PLR47_03470"
     CDS             3552..4202
                     /locus_tag="PLR47_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOG12028.1"
                     /translation="MKLFAVSDIHGATKPIERAAPLIRASDLVVIAGDITHTKTRAEA
                     AEVIACIEQFSGRILAVHGNWDKIEVKDLLEEKGYSLHGKGRMMGEIGFFGLGGSSPT
                     PIKTATTYTEEEIALVLQAGYEQVKDASQVVLISHVPPRGVRDRSFIGLRGGSRSVKA
                     FLEMHPVSLCLCGHIHEASGIERFQNTIVANSGSFKKGRYVSVEIGPNLAARPGRI"
     gene            4337..5212
                     /locus_tag="PLR47_03475"
     CDS             4337..5212
                     /locus_tag="PLR47_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOG12029.1"
                     /translation="MKLTSEFVGAPLKDYTCTVDARRTMNYAAAIGDAHPAYFDDERP
                     GGIIAPPLFPVALTWPILENITDYLQADAFPREVLLTQVHYSEHLSLHRPVTPGMRLK
                     IQGVIAAILPHRAGTYVVIRFDARDAEGRQIFTEHLGAMMRGVKCADAGKGGEKLPSV
                     PSRTNETILRESRILIDPLAPFIYDGCTNIYFPIHTSVQFAHQVGLPGIIHQGTATLA
                     LSVHHLLNQEADGDSRRLTTVFCGFSGMVIPGSSIRVCLTGREETAEATDLFFTVFNA
                     EGKKAIRDGYLRLKN"
     gene            5229..6839
                     /locus_tag="PLR47_03480"
     CDS             5229..6839
                     /locus_tag="PLR47_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG12030.1"
                     /translation="MIWEKLNQTWHYVEKWAHKTPDADALVFGDERLNWREFAAQMNA
                     VALAYLEMGIEKGDRIALLSMARNEFLTTYMAAGKVGAIWLGLSPKYTLEELRYQLND
                     CRPKLLITLREYMGADLSGTVSTLKKECPFLQNILVIGDPFDGAENFAAYIGKIRKHL
                     ADALEQRAADVRETDEALLLYTSGSTGKPKGVVHTHRSIVENIKVEVGKFYFDEKSRG
                     LLHFPINHVAADVEIGFGAIMSGGCIVCMDKFDPAQTLKVIEQEKITHIGQVPVMFLL
                     EFKQPEFWTCDFSHVQAFVWAGAAAPKVMIDLLEPICKKTGAKLITGYGSTEVCGFIT
                     YTEKEDDVETLLKTAGKIAMPFELKIVDGERRELPANEIGEIAVRGPFLMKGYYNKPE
                     ETARVLDQDGWYYTTDLAFKDDRGYIHITGRLSEMFKSGGENVYPREVEEVIESHAAV
                     LFAAVIGVPDDIYQEVGWAFAMPTPGQNVTEEDLRELCKNSLANFKVPKKFFIRPMLP
                     LLASGKVNKLALKEEIGGMLKKQQTGLS"
     gene            7054..9105
                     /locus_tag="PLR47_03485"
     CDS             7054..9105
                     /locus_tag="PLR47_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG12031.1"
                     /translation="METLHRGMACNQDEAALLKSEEMYRALVEGSQDAFLVLDDQFRI
                     VFVNEMVCRASGFSRRDFLGQSFMNFLPEENRAVVVERYQKRQQGEDPPSKYDFTYRM
                     KSGEDRHSELKTSVFRNSAGKVWTICQMSDVTELRRMQQALQKSEERLRRITDNMEDL
                     VLQVNLEGTIEFISPSVKQVLDYDIADLLGSSVISYIHEEDLASAMSVLKKGVKKGQN
                     KTEIRIRHRDGRFFWFEVVGTAIRNETGKITGAVLGGRDVTQRKEIENSLRQSEETFR
                     ALTESTVVAIFVVQGERFRYINKAFTLLSGYTMEDLREFHFWDLIAPEMRDFVRDQGL
                     ARQRGEEVVSNYEIKYLTKTGETRIGDFGATFIHYEGRPAMIASISDTTERKKKKEEL
                     RASEERYRTIIENIEDGYFEVNLKGDLIFASDPCMKITGTDRKTFIGMNFREFSPREN
                     WPAIYQAFYRVFETGEPLKGLSWEVVRIDGVHQYIEISTSLIRDHEGRPVGFRGIVRD
                     VTERKQREDMIQWMAYHDILTGLPNRALFYDRAAMVLAQAKRKQWRFGLMMLDLDRFK
                     EVNDTYGHDAGDKLLIGVARCLEEALREQDTVARIGGDEFVVILPEVSSREDVRMIRE
                     RIAKGFQRPIPIGETEMVAACSIGVALYPDDAGDIESLMHFADEAMYRAKIEGGGLCS
                     N"
     gene            9143..10048
                     /locus_tag="PLR47_03490"
     CDS             9143..10048
                     /locus_tag="PLR47_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG12032.1"
                     /translation="MKQQAKGKIHERITAIPNAWYPVYLVQGEKKNLMIDAGVNLLGP
                     RYLTLIQDILGGATKLHYLFLTHSHYDHVGSAGYLKRRIPGLKIGAHERVGGLLHKPS
                     VLDMMNRLSANHVELLKYNAAGEDLTLKPFDIDILLKQGDELDLGGLTCRVYETPGHT
                     KDSLTFYFPEIGVLFPGESAGVLAGETAGEMQVEFLASYRDYIDSLKLMISLKPGLIC
                     LGHGWVLGGRDAEDFLRRSLAETFRYRELIEDYLNAAGGDVETAIQDMAHAQYDVKGG
                     IFQERISYVTNLSAQVRHIAGLTAP"
     gene            10103..10873
                     /locus_tag="PLR47_03495"
     CDS             10103..10873
                     /locus_tag="PLR47_03495"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOG12033.1"
                     /translation="MSKFIDLSVAIESGLPSDPPMMIPKIMYVDHALGAESMKAFYPG
                     LTASDLPEGLGWALEILEVSTHAGTHMDAPWHYHPTQDRGKPALTIDQFPLEWGVGNG
                     VKLDFSGFPDGYNITVEDVQTKLKEINYSLRSGDVFLAQSGAAPFWGTPEYLVKGCGF
                     GRAATLWILDQGVHVVGTDGWSWDRPLPFQAEEFAKTRDASLIWEGHFAGIDKGYFQI
                     EKLANLEKIPSTGFRFYCFPVKIKGASAGWIRAVAEIL"
BASE COUNT         2537 a         2867 c         3239 g         2397 t
ORIGIN      
        1 ctggatctgc atggccgcct caaagccgga tccggccatc ttcagaaaaa tctccggcag
       61 cgcgttcatt gtattggcgg tggccgatgc cgattccggc tcgttcatca ttcgggaaaa
      121 ggatttgaac agattcaggc tggtttcaag ctgccttgtg aaccggtttt gcacgtctgt
      181 ctgcgttttc agcgtcgtgt ccgaatcggg cggaatggtt ttggccatgt tcagccacat
      241 atccgcggcg gtcttcagcc acgggaaaac ggacgattcg ttgaaaggga aggggttgcc
      301 tgcgtcagcc atgatgacct cttttcggag ccttgctctt gaaggtgttt ttggaatccc
      361 ttcagggccg gcccgcggtt tttttgtaat ctatccgccg gtgtttgtca atcggtttcg
      421 gccgccggcc cctgttccag cagcaaaggc cagggaagcg tcgggaaaac ggcgttgagc
      481 ggatggtcgt cgtccacgaa gtgatgctcc aggcgcgcga atgttttttc ggcaatggct
      541 ttgacggcct gtgaagggac gacgtcgtct cgcgttccgt gatacaggac cgtcgggatt
      601 tgcagttttt gccggaccgc ctgttcaaag ccttcaatgg tcagtgcggg ggcaagcaaa
      661 atcagctttt gaatcagcgg ttcgttatcc agcgcgaagc gggccgccat caggcctccg
      721 tagctggatc ccaccagaat cagattggtt ttgcccgcaa ggacgctttg aagcttttgc
      781 attcgggtcc cgaagccgcc cgtgtaatct tcaatgatca tttccgggaa cgcttttcga
      841 aagtactgag ccttgtttcc ctgggaagtg ctttccaaac cgtgaataaa aaccagcgtg
      901 tttttcatgg acatcctttc cggcggtgtg gcggatttca ttttgacgtt gcccggatta
      961 tgccattgtg ttaaatcgtg cgcaacctta ttttgaaaaa ggagatgtat atggctcagg
     1021 tattgccttt taaagcggtt cggccgcaga cgaaatatgc gtcgcaggtg gcggcgctgc
     1081 cttatgacgt catgacaaga gaagaggggc aaaaagcgac ggccggcagg ccgctcagct
     1141 ttatgcatgt ggagaaatcg gagatcgacg ttccggacgg cacgcgtccg gatgatcctt
     1201 tgatctatga gacggcgaaa cgcaactttg caaggctgcg ggaagaggga atcctgatcc
     1261 aggatgcggc cccctgctat tacgtttatc gccagaagat ggggcctcag gtgcagacgg
     1321 gcatcgtcgg cgtcatgagc gccgcggaat acgacgaggg taaaatcagg aagcacgaac
     1381 tgacccggca ggacaaggag gacgaccgca tccgccacgt cgatcaggtg aacgcgcaga
     1441 ccggcccggt attcatcagc tacaagacgc gacccggctt aaacgagatc gtggaaaaga
     1501 ttaccgccgg ccggccggaa tatgatttca ccgccgaaga cggcgtggtc cacaccgcgt
     1561 gggtggtcgc ggatcaggcg caggtagacg ccatccggtc gcagttcgat caggtggcag
     1621 ccctgtatat tgccgacgga catcaccgcg ccgccgccgg agctacggtt gcccgcagac
     1681 gccgcaagcc ttcgcagacg caggagtcgg acagcgtgct ggcggtgttt tttccccacg
     1741 atcagctcaa ggtcatggat tacaaccggg ccgtgaagga tcttcgcggt ctgtccgccg
     1801 aagcgtttct ggaaaaaatt tcctcctcgt tcaccgttac gaaggggttt gccgaaaaat
     1861 cgccgcggcg cctgcatgat ttcggaatgt atctgggcgg cgcgtggcac tgcctcacga
     1921 ttaaagaagg cgcctatgac gcgaacgatc cggtggccag cctcgatgcg gccatattgc
     1981 aggaccatct gctggcgccg gttctgggca ttgccgatcc gcgaaccgac gaccggatca
     2041 aattcatcgg cgggattcgg gggatggcgg agctcgagaa gctggtggat caggacggct
     2101 tcgccgtggc gttttcgctt tatgccacga cgatggagca gatcatgaac gtggcggatg
     2161 cgggagcgat catgcccccc aagtccacat ggttcgaacc caagctgcgc agcggtttgt
     2221 ttgtccactc cctggagtag gaaaagaatc cgcttcaggg gaatgtccgg atgctttctg
     2281 ccgcggggta atattttgtt tgacaggctt tgccgccgcg catcggcggg catgaaaggg
     2341 ggctcgaaga gcggacgatt ccggttgcct gaagccggac cggttacctg ccccaatggc
     2401 aggcaaagaa ctcgcatccg tccgacgcca gcaccgttgc ttctccccgg ggatggtgcc
     2461 cgtcgagatt ctctctgaaa acccgttccc actggccgcg cagcatgtgg tagtcattgc
     2521 gccggccgcg gggctcagtc agaaacgaga tcacctccgc catgacgaaa ttgtccacgc
     2581 agctctgcgt cacggtgatg ggggcgtcat gggcaaagat ggagtcgatg agcggcccgt
     2641 aggactggta aaccgacacg gccagctccg gagaaccaag ttgaggcgtg tcgttgtctc
     2701 cgtccatttc catcagcagc agatgattca ctttgccgct catttcccga aggtgcgccg
     2761 ctttggcgtc gcagggcata tggtggtagg caagcgacag aagcgcgatg tcgatgcccg
     2821 ccgggagttc cccggcgtgt tcctggattc ggccgtggcg cggatcaacg gtcagctccg
     2881 ggaaagcctt tcgaagcttt ccttccgcga cctccatcat ggacgccgaa gaatccacca
     2941 ggatggcccg ggcgatattt tgcgcgcggc cggttttcat caggcgctgc aacagaagca
     3001 ccagaaggct gccgtcgccg cagcctacgt ccagaaccgt caggggatct gcaaagcgag
     3061 ggagcgagga agaagcaaac cggagaaatt tttcccgcat gccgacgatc gccggcaccg
     3121 tggtaaaatg ctcatagtcg cgcggatcgg caaaaatttt ttccggcatg taaaaccggc
     3181 cgttgtgccg ctccaggtag gaagccatga tggcgttgaa gattccgtct tcttcgagcg
     3241 ctccttcccg gaaccagtcc gctgcttccg gccgctttcc cagaccggcc agcaggccgc
     3301 ccatataaaa atgaaaatgc gcgcgaagag cattgcgaat gccgtccagt gaggagcggg
     3361 gagtgagttt tccgtccatt gcctgccggg catgttcttc ccagaccgct tgttcatcgg
     3421 atgagagata atacatgatt tgagccttgc agtagatgta ttttgggagc aactataaga
     3481 aagacgaaac ggcgtgtcaa gggaatcctg atttatcgac attgaaatga caaagcaaag
     3541 ccgggagggc tatgaaacta tttgctgtaa gcgatatcca tggggcaacc aaacccattg
     3601 aaagggctgc gccgctcatc cgcgcatcgg acctggttgt cattgccggg gacattaccc
     3661 acaccaaaac acgcgccgaa gcagccgagg tcatcgcctg cattgaacaa ttttcgggcc
     3721 gcatcctcgc tgtccatggc aactgggaca aaattgaggt taaagattta cttgaagaaa
     3781 aaggctacag ccttcacggg aagggaagga tgatggggga aatcggtttt ttcggtctgg
     3841 gcggatcttc gcctacgccc atcaaaacgg ccaccaccta cacggaggaa gagatcgctt
     3901 tggtgcttca ggcgggatac gagcaggtaa aagacgcgtc gcaagtcgtc ctgatctcgc
     3961 atgttccgcc acggggagtc cgggacagat ccttcatcgg tctcagaggc ggcagccgca
     4021 gcgtgaaggc gtttcttgaa atgcaccctg tctcgctgtg cctgtgcggc catattcacg
     4081 aggcgtccgg gatcgagcgt tttcagaaca cgatcgtggc caattcgggc agctttaaga
     4141 aaggccgata cgtatccgtc gaaatcggtc cgaaccttgc ggccaggccc ggaagaattt
     4201 aatgcatgaa ttgacgaata ccgattatta tgcaatttta ctttccgcga atgcatcatt
     4261 ttcccgatga accattccac tgtgaattgc gtagctgtcg ggaaaaatga gcaaaaattc
     4321 cgtctggaga gattttatga aattaacctc cgagtttgtc ggcgcgcctt tgaaagacta
     4381 cacctgcacg gtggacgcgc gccggaccat gaattatgcg gcggcgatcg gcgacgccca
     4441 tccggcttac tttgacgatg aacggcccgg gggaattatc gctccgcccc ttttccccgt
     4501 ggccttgacc tggcctatcc tcgagaacat tacggattat ctgcaggcgg atgcgtttcc
     4561 ccgggaggtg cttctcactc aagtccacta tagcgagcat ctcagccttc accgaccggt
     4621 gacgcccggc atgcgcctga agattcaagg cgtcatcgcc gccatcctgc cgcatcgggc
     4681 cgggacctat gtggtgatcc ggtttgatgc gcgcgatgct gaaggcaggc agatattcac
     4741 ggaacatctc ggcgcgatga tgagaggcgt taagtgcgcc gacgccggaa agggcggcga
     4801 aaaacttcct tctgttcctt cacggacaaa cgagacgatc ctgcgggaat cccgcatttt
     4861 gattgatccg ctggccccct ttatttatga cggatgcacc aatatttatt tccccattca
     4921 tacctccgtt caattcgcgc accaggtggg gctgcccgga atcattcacc agggaacggc
     4981 cacgctggcc ctgtccgtcc atcatcttct gaatcaggaa gcggacggcg attcgcggcg
     5041 cctgacgacg gtcttttgcg ggttttccgg aatggtgatc cccggttcat ccatccgggt
     5101 ttgcctgacc ggcagggagg aaacagcgga agcgacggat ctctttttca ccgtttttaa
     5161 cgccgaaggg aaaaaagcga tccgtgacgg gtatctgcga ctgaaaaact aaaaagggag
     5221 gaagcatcat gatctgggaa aaactgaatc aaacatggca ctatgttgaa aaatgggccc
     5281 ataagacgcc ggatgccgat gcgctggttt ttggcgatga gcgcctgaac tggcgcgaat
     5341 ttgcggctca gatgaatgcc gttgcccttg cttatctgga aatggggatt gaaaaaggcg
     5401 accggatcgc gcttttgtcc atggcgcgca atgaatttct cacgacctac atggctgcgg
     5461 gaaaagtcgg cgcgatctgg ctggggcttt ctccgaagta cacactggag gaactccgtt
     5521 atcagctcaa cgactgccgc cccaaattgc tgatcaccct gcgggaatac atgggcgccg
     5581 atctttccgg gacggtcagc acgctcaaaa aagagtgtcc cttcctgcaa aacattctgg
     5641 tgatcggcga tccgtttgac ggcgccgaaa attttgcggc atacattggg aaaattcgga
     5701 agcacctggc tgacgcgctt gagcaaagag cggcggacgt ccgggaaacg gacgaagccc
     5761 tgctcttata cacgtcggga tccaccggca agcccaaagg agttgtgcat acgcatcgca
     5821 gcattgtgga aaatataaag gtggaagtcg ggaaattcta ttttgatgaa aagtcgcggg
     5881 ggcttctcca ctttcccatc aatcatgtgg cggcggacgt cgaaatcgga ttcggggcca
     5941 tcatgtccgg aggctgcatc gtttgcatgg acaagttcga tccggcccag acgttgaagg
     6001 tgatcgagca ggaaaaaatc acccacatcg gacaggtgcc ggtgatgttt ctcttggaat
     6061 tcaaacagcc ggaattctgg acctgcgatt tcagccatgt tcaggcgttt gtctgggcag
     6121 gagcggccgc gccgaaagtc atgatcgatc tgctcgaacc gatctgcaaa aaaacagggg
     6181 caaagctcat caccggctac ggaagcacgg aggtatgcgg cttcatcacc tatacggaaa
     6241 aagaagacga tgtggaaacg ctgctgaaga cggccggcaa aatcgcgatg ccttttgaat
     6301 tgaaaatcgt ggatggggaa cgccgggagc tgcccgcgaa cgaaataggc gaaattgccg
     6361 tgcggggtcc gtttctgatg aaaggatact acaacaagcc ggaagaaacg gccagggtat
     6421 tggatcagga cggctggtat tatacgaccg atctcgcttt taaagacgac aggggataca
     6481 tccacatcac cggaagactt tcagagatgt tcaaaagcgg cggcgagaat gtctatccgc
     6541 gcgaagtgga agaagtgatc gaatcgcatg cggcggtcct gtttgcggcg gtaatcggcg
     6601 ttccggacga catctatcag gaagtcggct gggcctttgc catgccgacg ccggggcaaa
     6661 acgtcacgga agaggatctg cgcgaattgt gcaaaaactc cctggccaac ttcaaggttc
     6721 cgaagaagtt ctttatccgg ccgatgcttc ccctgctggc gagcggcaag gtgaacaaac
     6781 tggcgctgaa agaagaaatc gggggaatgc tgaaaaaaca gcaaacagga ctgtcttaag
     6841 aaacatcaat aaaaacggat ttttcccgtg tcgaggcgca tcggacgctc cttgcggatc
     6901 tttgcgtttc cggcacattt gccttgacag atatgctcaa tttatatacg cattctatgc
     6961 gcgcatacat tgctgaattg aaaaattaac tcgtttttcc ggcatcatca tgcctttatt
     7021 ttgaattcgg cttttgggta agtgatacgc acaatggaaa cattgcaccg tggaatggcc
     7081 tgcaaccagg atgaagccgc acttctgaag agcgaagaaa tgtatcgcgc actcgtcgaa
     7141 ggttctcagg atgcctttct ggttctcgat gatcaattcc gcattgtttt tgtcaatgaa
     7201 atggtctgcc gcgccagcgg attttcccgc cgggatttcc tgggccaaag tttcatgaat
     7261 tttcttcccg aggaaaaccg ggccgttgta gttgaacgct atcagaagcg tcagcagggt
     7321 gaggatccgc catccaaata tgactttacc tatcggatga agagtggaga agatcgccac
     7381 tccgaactga aaacctccgt ttttcgcaac agcgcgggaa aagtgtggac gatctgccag
     7441 atgagcgacg tcacggaact gcgccggatg cagcaagccc ttcagaaaag cgaggagcgg
     7501 ctccgccgga tcacggacaa tatggaggac ctggttctcc aggtcaacct ggaggggacc
     7561 atcgaattca tctctccgtc ggtcaagcag gttctggact acgatattgc cgatctgctc
     7621 ggtagttcgg tgatttccta catccatgaa gaagatctgg ccagcgccat gtcggttttg
     7681 aaaaagggcg taaagaaagg tcagaacaaa accgaaatcc gcatccggca tcgggacggc
     7741 cgctttttct ggtttgaagt ggtcggcacg gccattcgca acgaaacggg gaaaattacc
     7801 ggcgccgtac tgggcgggcg ggatgtcacg caaagaaaag aaatcgaaaa cagcctgcgc
     7861 cagagcgagg aaacgttccg ggcgctgacg gaaagcacgg ttgtcgcgat tttcgttgtc
     7921 cagggcgaga gatttcgcta catcaacaag gcttttacgc ttctttccgg ctacacgatg
     7981 gaagatttgc gggaattcca tttctgggac ctcatcgctc cggagatgag agattttgtc
     8041 cgggatcagg gactggctcg ccagcgcggg gaagaagttg tatcaaatta tgaaatcaaa
     8101 tatctgacaa aaaccggcga gacaagaatc ggcgatttcg gggcgacctt tatccattat
     8161 gaaggccggc cggccatgat cgcttcgatt tccgacacca cggagcggaa aaagaagaaa
     8221 gaagaattgc gggccagcga ggagcgttac cgcacaatta tcgagaatat cgaggatggc
     8281 tatttcgagg tgaatctcaa aggagatctt atttttgcca gcgatccctg catgaaaatt
     8341 accggcaccg accggaaaac cttcatcggc atgaattttc gcgagttctc gccgagggaa
     8401 aactggcccg cgatttacca ggcgttctac agggtttttg aaaccggaga gcctttgaaa
     8461 ggcctgtcct gggaagtggt tcgtatcgat ggggttcatc agtatatcga aatatccaca
     8521 tcgctgattc gggatcacga ggggcggccg gtcgggttcc ggggcatcgt acgggacgtg
     8581 acggagcgca agcagcgcga agacatgatc cagtggatgg cttaccatga cattctgacg
     8641 gggcttccca accgggctct tttttacgac cgcgccgcca tggtgctggc ccaggccaaa
     8701 cggaagcaat ggcgtttcgg actcatgatg ctcgatctcg atcgcttcaa ggaagtcaac
     8761 gacacctacg gacatgatgc cggagacaag ctcctgatcg gggtggcccg ctgcctggaa
     8821 gaggcgctgc gggagcagga tacggtggcc cgcatcggcg gggacgagtt cgtcgtcatt
     8881 cttccggaag tttccagccg cgaggacgta agaatgatcc gagagagaat cgccaaaggt
     8941 tttcaaaggc cgattccgat cggagagacg gaaatggtcg cggcctgcag catcggcgtg
     9001 gccctttatc ctgatgatgc cggggatatt gaaagtctga tgcactttgc ggacgaggcg
     9061 atgtacaggg ccaaaatcga aggaggcggt ctttgcagca attgatgcca gagaggcttc
     9121 cgtccgcccg gtccggttct ttatgaaaca gcaggccaaa ggcaaaatcc acgagcggat
     9181 caccgccatt ccgaatgcct ggtatcccgt ttatctggtt cagggagaaa agaaaaatct
     9241 gatgatcgac gcgggcgtta atcttctggg tccccgctac ctcacgctca ttcaggatat
     9301 tctcggcggc gcaacaaagc tccactatct ttttctaacc cattcgcatt atgatcatgt
     9361 cggctctgcg ggctatttaa aacggcgcat tccgggctta aagatcgggg ctcatgagcg
     9421 ggtcggcggc ctgctgcaca agcccagcgt tctggacatg atgaaccgcc tcagcgccaa
     9481 ccatgtcgag cttctgaagt acaatgccgc cggtgaagat ctgacgctga agccgtttga
     9541 catcgatatt ctgctgaaac agggggacga actggatctg ggcggtctca cctgccgcgt
     9601 ttatgagacg cccggccaca ccaaagactc gctgacgttc tactttccgg agatcggggt
     9661 cttgtttccc ggcgaatccg ccggcgtcct tgcgggtgaa acggccggcg aaatgcaggt
     9721 tgaatttctt gcctcctacc gggactatat cgattcgctg aaactcatga tttctcttaa
     9781 gcccggcttg atctgcctgg ggcatggatg ggtgctgggc ggcagggacg cggaagattt
     9841 tctccggcgc tctcttgcgg aaaccttccg gtaccgggag cttatcgaag actatctgaa
     9901 tgccgcgggc ggggacgtgg agacggccat tcaggatatg gcccatgccc aatacgacgt
     9961 caagggcggc atctttcagg aacggatttc ctacgtgacc aatctgagcg cgcaggtcag
    10021 gcatattgcc ggattgactg caccctgaat ccttccggcg atcaccatca acgaagcaac
    10081 gactgatatg gggaggatga tcatgagcaa gttcattgac ctttcagtgg cgatcgaaag
    10141 cggcctgccc agcgatccgc cgatgatgat tcccaaaatc atgtatgtgg atcatgcgct
    10201 gggggctgaa agcatgaagg cgttctatcc cggcctgacc gcttccgacc tgccggaagg
    10261 attgggttgg gcactcgaaa ttctggaggt ctcgacgcac gcgggtacac atatggacgc
    10321 accctggcat tatcatccca cacaggaccg ggggaaaccg gctttgacca tcgatcagtt
    10381 tcccctggaa tggggagtgg gaaacggcgt gaagcttgac ttttcgggct ttcccgacgg
    10441 atacaatatc acggttgaag atgttcaaac caaactcaag gaaataaatt actcgcttcg
    10501 gtcgggggat gtttttctgg cgcaaagcgg cgccgcgcct ttctggggca cacccgaata
    10561 tcttgtaaag ggatgcggat ttggccgggc ggcgactctc tggattctgg accagggcgt
    10621 tcacgtggtc gggacggacg gctggagctg ggacaggcct ctgccctttc aggccgaaga
    10681 gtttgcgaag accagggacg cttcgctgat ctgggaaggg cattttgcgg ggatcgacaa
    10741 gggctatttc cagattgaaa agctggccaa tctggaaaaa attccgtcaa ccggcttcag
    10801 gttttactgc ttcccggtga aaataaaggg cgcgagcgcc ggatggatca gggcggtcgc
    10861 ggagatttta taaaacggac ccgatgggcc tggttttccc gatggaggcg tgaaatgacc
    10921 ggcgcctatg ttccgtaaac actattagtg agttacggcc tgttgcccaa gcgaaaaggg
    10981 tattcgggca acaagccgcc agcactgggc gcgtggtttt gataaaaaga ccccctcagt
//
LOCUS       DAOSIM010000046        10738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_6032_length_10738_cov_7.052513, whole genome shotgun sequence.
ACCESSION   DAOSIM010000046 DAOSIM010000000
VERSION     DAOSIM010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 10738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10738
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6032_length_10738_cov_7.052513"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1557)
                     /locus_tag="PLR47_03500"
     CDS             complement(<1..1557)
                     /locus_tag="PLR47_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOG12034.1"
                     /translation="MSSEKTFPMSRAVLGAYAQGKEDETLLPLVLVDQKQAPLGRIQP
                     GDSIIFYNIRGEREIELTRSFTEENFREFPVHPALPVHFATMIEYSKGLNADVAFPPQ
                     GVLSDTLSDIIAAHGLAQAKITEAEKAVHVSYFFNGKKSDPLPKEERIVVPTRKDVRL
                     FDEAPEMSIDEITRAAGAKIQDPSCRFIVVNFPNVDVVGHIENEPAILRAVSAVDRHA
                     GLLVEQALRRNMTVIVTADHGTVEKWLYPDGGVDTGHTDSPVPFILLHPEQKPALKPE
                     GELTDIAPTILDLLGLPVPDSMTGSSLIQEKPPSLAPASRLLLLILDGWGLCDSQKGN
                     LIACAETPVMDRLLRDYPHARLAASGLAVGLPAGTVGNSEAGHLHIGAGRRIYSDRLM
                     IDQSIARRDFHRNPAFTRAMNLARQNRRALHLMGIVSFFSSHGSIEHLFALMDMAAEQ
                     GVSELYLHSMLGRRGEQAESGARYIRQVEEKCRSLGIGQVVSVIGRYWSMDREENWDR
                     IEKTYRMLVYG"
     gene            1722..2117
                     /locus_tag="PLR47_03505"
     CDS             1722..2117
                     /locus_tag="PLR47_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12035.1"
                     /translation="MVQVLLKGREKQQENLYEELLRERAAVLARAGFAVEDALEKLEK
                     LDRRIGEMMNRCQSLQTGSPVGAHPREQQSVFEEIDEIIDQFNATCQKAELQYYYLIV
                     TREALGLRRHETVQKFYRIPSKKKKMRAL"
     gene            2117..2770
                     /locus_tag="PLR47_03510"
     CDS             2117..2770
                     /locus_tag="PLR47_03510"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOG12036.1"
                     /translation="MDRYKKQRIRMVEAQIRARAVRDESVLKVMETVPRHLFVDEGLI
                     NQAYSDNPLPIGERQTISQPYIVALMTQALELKGMERVLEIGTGSGYQTAILAKLADR
                     VFSIERVAVLASRARRILDQLNCYNVAIRVGDGSYGWKEEAPFDAIITTAAAPQLPRH
                     LLDQLAVGGRMVVPVGGREAQTLYKITRSSENPSELKREDLGGCRFVSLIGESGWKE"
     gene            2758..3669
                     /locus_tag="PLR47_03515"
     CDS             2758..3669
                     /locus_tag="PLR47_03515"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG12037.1"
                     /translation="MERMSRHILPRRHFAVFRRRYFGAYLILSMLVLLMPAGCANSQV
                     HRSQASGVYHIVKKGETLYGIARAYSASLQDVAEINNIEDVSNIREGAVIFIPNADQV
                     IEDVLVSSGKAAADEKPSVRKPPPVSAEVLPPPGKIPAKPAKPSRPEVQSVPPGPKSE
                     KTKAPVEEKPQISEEKVVMDKGRFAWPARGSVKTRFGIQPNKTYHNWIKIACPAGTRV
                     KAAAFGTVIFSANLKDFGETVIIRHEKDFATVYTHLKKRSVQADQDVGIGQTIGLAGR
                     ADEAGETYFNFEIRLKGKARNPLFYLP"
     gene            3794..4279
                     /locus_tag="PLR47_03520"
     CDS             3794..4279
                     /locus_tag="PLR47_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOG12038.1"
                     /translation="MFKVGDLAVYPAQGVGVIEAIENREVMGKKQPFYVMKIMGNGMK
                     IMIPTNSAKAVGLREVILEKEIPKVYEILRNKDVTIDKQTWNKRYREYLEKIKTGSVF
                     EIASVLRDLFILKSDKNLSFGERKMMDTAKNLLVKEISVATNAQEAKVERDLQMIFTV
                     Q"
     gene            4451..5479
                     /locus_tag="PLR47_03525"
     CDS             4451..5479
                     /locus_tag="PLR47_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOG12039.1"
                     /translation="MIRLIVRALLILICSVSGYFIAYHTMAAAGSYAVFQAIFGLIIG
                     LVVAMLVIRIERDIRRQSVRTIAGGVAGMITGLLIALIIGFGLNMIIRIPPNQQIVPW
                     IYLLMIGILGYLGLVIGSKKAEEISFRTTDSRKLMDHRLLDTSVIIDGRIADICDTGF
                     VDGELIVPRFVLNELQFIADSSDSMKRSRGRRGLDVLNRMQKSTAINIEIVEQDFPKI
                     KGVDGKLVALAQKLNAKLLTNDYNLNKVAELQGVRVLNVNELANAMKPVVLPGEQMTV
                     KIIREGKEQGQGVGYLDDGTMIIVDSAQKMINMTVDVIVTSVLQTTAGRMIFTELKEA
                     ADRKSGNA"
     gene            5575..6765
                     /gene="ispD"
                     /locus_tag="PLR47_03530"
     CDS             5575..6765
                     /gene="ispD"
                     /locus_tag="PLR47_03530"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOG12040.1"
                     /translation="MKTVAIIPAGGSGRRAGASIAKQYLLLHGVPLLARTLRVFQNAE
                     AISAIVLVVPTPDVESVRERIVEKYSLTKVAAVVAGGGERQDSVASGLRAVPPDCDIV
                     MVHDAVRPFVTGEMIDRVAAAAACCGAASLGVPAKDTIKETTDDGIVTRTFPRSRLWQ
                     TQTPQAFACDLLRRAHASAKEDHYYGTDDASLVERIGAKVQMIAGSGENMKITTPEDM
                     KMAESLLCAGRGERIRRPSGLGYDSHRFAKDRKLVLGGVDIPFDRGLAGHSDADALLH
                     AVCDALLGMAGEGDIGRHFPDHDPAYKNISSLILLERVGKMVESRGVSIENIDATLVL
                     EKPRLAPYAQAMAANIARVLKIPETAVNIKAKTNEGMGFTGRGEGVAVWAVASGAERI
                     ADGE"
     gene            6755..8206
                     /gene="gltX"
                     /locus_tag="PLR47_03535"
     CDS             6755..8206
                     /gene="gltX"
                     /locus_tag="PLR47_03535"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOG12041.1"
                     /translation="MENKARVRFAPSPTGELHVGNARTALFNWMFARHEGGRFILRIE
                     DTDESRSDSAYQTSLLEDLKWLGLTWDEGPDIGGGYGPYRQSERLDLYEAHLKKLVDA
                     DLAYPCYCTEEELEEERQTLILAKRMPRYLGKCRNLSAGEREALAASGRKPSCRFKVP
                     RTTIEFNDLIRGPIRFEGEAMGDFIIVRSNGMPAYNFAVVIDDHRMAVTHVIRGEDHL
                     SNTALQIMLYRALGFEPPAFAHHCLILGKDHAKLSKRHGSVSVGEFRRQGFLPEALIN
                     YLGLLGSSFAGGREILSRDEMIDAFSLSRASRSGAVFDEDKLRWLNAAYIRKTSTEDL
                     LRKLEPFLRAAGFRPETLDAAWLKNVLETVKGELTTLAEIGAHIDLFFDERYSLSDEA
                     RRLLAAPGAREVVGAFGDYLERAEDNAGDIYAAAIRHAKEKSGAKGRDLFMPVRAALT
                     GKLKGPELDRVFAVLGVDSARGRFQQVKKTLGI"
     gene            8404..9030
                     /locus_tag="PLR47_03540"
     CDS             8404..9030
                     /locus_tag="PLR47_03540"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG12042.1"
                     /translation="MDSGKRNFDQDAARWDLNPARVKLASEIGSAILREISLLPDSDV
                     LDFGCGTGLLTLMLQPHVRSITGVDSSQGMLDILNRKISERNLTNVITRYADLDQGDV
                     LSGAFHLAVSSMTLHHIRDVAPLLKRFCHLLHPSGLLCIADLDEDGGRFHTSSEGVFH
                     FGFDRQKLARQFEEAGFQNVRAVQAASVEKPVAGTMQRFTVFLMIGRK"
     gene            9197..10555
                     /gene="radA"
                     /locus_tag="PLR47_03545"
     CDS             9197..10555
                     /gene="radA"
                     /locus_tag="PLR47_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOG12043.1"
                     /translation="MKKSKTSFFCQHCGYMSPKWLGKCPSCSGWNCFAEELVTEPDSV
                     SRADRVFGGKPMPIFDIPAVEGERTSTGIVEVDRVLGGGIVSGSAILIGGEPGIGKST
                     LMLQVMKNLAMGGRKVLYVSGEESASQIKLRADRIGAQAKDLLVLVEVSLEKILEQIR
                     KTEPAMVVIDSVQTVYSSDLMSAPGSVGQVREASSRLILAAKKSSIPVFLVGHVTKDG
                     SIAGPKVLEHMVDTVLYFEGDSGHAYRIVRSMKNRFGPANEIGVFEMRDRGLQEVPNP
                     SAFFLAERPENAPGSVVLASLEGTRPILVEIQALVSQSNLGMPRRTAIGVDHNRVSLL
                     VAVLDKICGLHLGGSDIFLNVAGGVRVEEPAVDLAVVSAMASSFLDRPVDARTVILGE
                     VGLTGEVRAVSQIETRVKEAARLGFNRCIVPKTNIASLGKAAKMEIQTITSLKELLDI
                     LF"
BASE COUNT         2418 a         2844 c         3230 g         2246 t
ORIGIN      
        1 tccgtaaacc agcatccggt aggttttttc gatccggtcc cagttttctt cgcggtccat
       61 cgaccagtat cgtccgatca cggacacgac ctgcccgatt cccagcgagc ggcatttttc
      121 ctccacctgc cgaatatagc gcgcgccgct ttcggcctgc tcgccgcgcc gccccagcat
      181 ggaatgaaga tacagctcgg agacgccctg ctctgcagcc atgtccatca gggcaaacag
      241 atgttcgatg gacccgtgcg agctgaaaaa agacacgatg cccatgaggt gcagcgcacg
      301 gcggttttgc cgggccaggt tcatggcgcg cgtgaaagcc gggtttcggt gaaaatccct
      361 gcgggcaatc gactgatcga tcatcaggcg gtcggaatag atccgccggc ccgcgccgat
      421 gtgcagatgc cccgcttcgg agttgcccac ggtcccggcg ggaagcccca cggccaaacc
      481 ggaggcggcc agccgcgcat gggggtaatc ccgaagcagc cggtccatca ccggcgtttc
      541 ggcgcaggcg atcaggttgc ccttttggct gtcgcaaagc ccccagccgt ctaaaataag
      601 cagaagcaga cgggaagcgg gggcgaggga aggaggcttt tcctgaatca aagacgatcc
      661 ggtcatggaa tcggggacgg gcaggcccag caaatccaga atcgtcggcg cgatgtcggt
      721 cagttctcct tcgggtttca gggcgggttt ttgttccggg tgcagaagaa tgaagggcac
      781 ggggctgtcc gtgtgtccgg tatccacgcc gccgtccgga tacagccatt tttccacggt
      841 gccgtgatcc gcggtgacga tgacggtcat gttccggcga agcgcctgct ccacgagcaa
      901 tccggcgtgc cggtccaccg ccgaaaccgc ccgaaggatc gccggttcgt tttcaatatg
      961 gccgacgacg tccacgttcg ggaagttgac gacgatgaaa cggcaggagg ggtcctgaat
     1021 tttggcccct gccgcgcgcg tgatttcatc gatggacatt tcgggcgctt cgtcgaacag
     1081 cctgacgtcc ttgcgcgtcg ggacaacgat tcgttcttct ttcggcaggg gatcgctttt
     1141 cttcccgttg aagaaatagc tgacatgcac ggctttttcc gcttccgtta tttttgcctg
     1201 cgcgaggccg tgcgctgcga tgatgtcgct cagagtgtcg gaaaggacgc cctggggagg
     1261 aaatgcgacg tcggcgttca gccctttgga atactcgatc atggtggcga agtgcacggg
     1321 gagcgccggg tgaacgggga attcccggaa gttttcttcc gtgaagctgc gggtcagttc
     1381 aatttcgcgc tctccgcgaa tattgtagaa aatgatggag tcacccggct ggattcttcc
     1441 cagcggggcc tgcttttgat cgaccagaac cagcggaagg agcgtttcgt cttcctttcc
     1501 ctgcgcgtag gcgccaagga cggcccggga catgggaaat gtcttttcag agctcatcga
     1561 aaattccggc taaacgttga atgatatcgg gcaggctatg taacgaaata atgtcgaact
     1621 gtaaaggaaa aaaatatccg tccggcgggc catgagcgat ttgaacttga aaattaagcg
     1681 cttgaatgat agaggccctc agaaatcgtt aagaaggctc tatggttcag gtactgctca
     1741 aaggaagaga aaaacagcag gaaaatttgt atgaagaact gttgcgggag agggcggcgg
     1801 ttcttgccag ggcgggtttt gccgtcgagg acgcgcttga gaaactggag aagcttgacc
     1861 ggcgcatcgg agaaatgatg aaccgctgcc agtcgctgca aaccggctcg cctgtcggtg
     1921 cgcatccgcg ggaacagcag tcggtttttg aagaaatcga cgaaatcatt gatcaattta
     1981 acgcaacctg ccaaaaagcc gagcttcagt attattattt gatcgtgacc cgtgaagccc
     2041 ttgggttgcg ccgtcacgaa acggtgcaga aattctaccg gattccctct aaaaagaaaa
     2101 agatgcgagc gctttaatgg acaggtataa aaaacagcgc attcgaatgg tggaagcgca
     2161 gattcgcgcc cgtgccgtac gggacgaaag cgttttgaag gtaatggaga cggttccgcg
     2221 tcaccttttt gtggacgaag ggctgattaa ccaggcctac agcgacaatc cgctgcccat
     2281 cggggagcgg cagacgattt ctcaacccta cattgtcgcc ctgatgacgc aggccctgga
     2341 gctcaaaggc atggagcggg ttctggaaat cggcacggga tccggctacc agacggccat
     2401 tctggccaaa ctggccgacc gggtcttttc catcgagcgc gtggccgtgc tggcctcgcg
     2461 ggcccgccga atcctggacc agctgaattg ctacaatgtc gccattcgcg tcggcgacgg
     2521 gtcctacggg tggaaggagg aggcgccttt cgacgcgatc attacgaccg ccgccgcccc
     2581 gcagcttccc cggcatctgc tcgatcagct tgctgtcggg ggcagaatgg tggtgccggt
     2641 tggcggccgg gaggcgcaga cgctttataa aattacccgc tcgtccgaaa atccgtccga
     2701 gctgaaaagg gaagacctgg ggggctgccg ctttgtcagc ctgatcggag agagcggatg
     2761 gaaagaatga gccggcacat tctcccccgc cggcattttg ccgttttcag acgccggtat
     2821 tttggggcgt acttgatttt gtccatgctg gtgcttctga tgcccgccgg gtgcgcgaat
     2881 tcccaggtgc acaggagcca ggccagcggc gtttatcaca tcgtcaaaaa gggggaaacc
     2941 ctgtacggca tcgcccgcgc ttattcagcc agcctgcagg acgtggcgga aatcaataac
     3001 atcgaggatg tctccaacat ccgcgaaggg gccgtcattt ttattcccaa cgccgatcag
     3061 gtgatcgaag acgttttggt atcctcgggg aaagccgccg cggacgagaa accatccgtc
     3121 aggaaaccgc ctcccgtttc cgccgaagtt ttgccgccgc cgggaaaaat ccccgcaaaa
     3181 ccggccaaac catcccgacc ggaggtccaa tccgttccgc ccggaccgaa atcggaaaaa
     3241 acaaaagcgc ccgttgagga aaaaccgcaa atttcggaag aaaaagtggt gatggataaa
     3301 ggccggtttg cgtggcccgc caggggatcc gtgaaaaccc gctttggaat tcagccgaac
     3361 aaaacctacc ataactggat caaaatcgcc tgtccggccg ggacaagggt gaaagcggcg
     3421 gccttcggga ccgttatttt ctcggccaat ttaaaggatt tcggcgaaac cgtgattatc
     3481 cggcatgaga aagacttcgc gacagtctat acgcacctga aaaaaagatc cgtccaggcg
     3541 gaccaggatg tggggattgg ccagaccatt ggactcgccg gaagagccga tgaagcgggg
     3601 gaaacgtact tcaattttga aatccgcctc aagggcaaag cccggaatcc cctgttctat
     3661 ctgccttgaa tgaccgccgt ttaggcctct ccaaaacatt tctgaagtat ttcagtaaat
     3721 tgtattaatc attgactaaa tcaagcaaaa gtgataatgt tcaacgttct taagaaaggc
     3781 agggaaatgt ccaatgttta aagtaggtga tttggccgtt tatccggctc agggcgttgg
     3841 cgtcattgaa gctattgaaa atagagaggt tatgggaaag aaacagccct tttatgtgat
     3901 gaaaatcatg ggcaacggca tgaagatcat gattccgacc aattccgcca aggcggttgg
     3961 gctcagggaa gtcattctgg agaaagaaat acccaaggtt tacgagatcc tgcgcaacaa
     4021 agacgttacc atagacaaac aaacctggaa caagcgatac cgggagtacc tggagaagat
     4081 caaaaccggg tctgttttcg aaattgcaag tgttttaagg gacttgttca ttttaaaaag
     4141 cgacaaaaac ctttctttcg gcgagcggaa gatgatggat accgcaaaaa atctgctcgt
     4201 aaaggaaatt tccgtcgcca cgaacgcgca ggaagccaag gttgaacgcg atttgcagat
     4261 gattttcacg gtgcagtagc aagcgggtgc gtggtctgtc tctttttcgc tctttgaaaa
     4321 ctgaaaatgg acaaacccta tttccctgaa tcgtttacgg cccctccacg gtcaacaagc
     4381 agtacggaag catgattcgc tcatttcatt cctgattcat cattatccat tgaatttatt
     4441 ttgaagggag gtgatacgat tgatcgtaag agctttactg attttgattt gttcggttag
     4501 cggttatttc atcgcctacc acacgatggc ggcagccggc tcctacgctg tttttcaggc
     4561 aattttcggc ttaattatag gcttggtcgt tgcgatgctg gttattcgga tcgaacgaga
     4621 tatccgcaga cagtccgtaa ggaccatcgc aggcggcgta gccggcatga tcaccggcct
     4681 cctgatcgcg ctgatcatcg gctttggatt gaacatgatc atccgaattc cgcccaatca
     4741 gcagattgtt ccatggattt acctgcttat gatcggcatt ctggggtacc tgggactggt
     4801 gatcggctcg aaaaaggcgg aggaaatcag cttccgcact acggattcga ggaagctgat
     4861 ggatcaccgc ctgctggaca ccagcgtgat catcgacgga agaatcgcgg acatctgcga
     4921 tacgggtttc gttgacggag agctgatcgt tccgcgtttt gtcttgaacg agctgcagtt
     4981 tatcgcggat tcttcggatt ccatgaagcg ctccaggggc cgccgcggac tggatgttct
     5041 caaccggatg cagaaaagca cggccatcaa tattgaaatt gtcgagcagg attttcccaa
     5101 aattaaaggc gtggacggga aactggtggc cctggctcag aaattgaacg ccaaactgct
     5161 gaccaacgac tacaacctga acaaggttgc cgaactgcag ggcgtgcgcg tcctcaacgt
     5221 caacgaactg gccaacgcca tgaagccggt ggtcctgccc ggagagcaga tgacggtgaa
     5281 aattatccgg gaaggcaagg aacagggcca gggcgtcggc tatctggacg acgggaccat
     5341 gattatcgtg gacagcgcgc agaaaatgat caacatgacc gtcgatgtca tcgtgaccag
     5401 cgtgctgcag acgacggccg gccgaatgat cttcacggag ctgaaggaag ccgccgacag
     5461 gaaaagcggc aatgcatagg catctttaag cgcaggtttt ttggccggga agaaagatat
     5521 cctgaacagg cccggaaaat ggttttccgg gcctgttttt gcaagagatt cgctatgaaa
     5581 accgtggcca ttattcccgc cggcggctca ggcagaaggg ccggcgcatc aatcgccaag
     5641 cagtacctcc tgctccacgg ggtgcccctg ctggcgcgca cgcttcgcgt gtttcagaac
     5701 gcggaggcaa tttccgccat cgtgctggtc gtgccgacgc cggatgtcga atccgtccgg
     5761 gagcggatcg tggagaagta cagcctcacc aaagtggccg ccgttgtcgc cggaggcggg
     5821 gagcgtcagg attccgtcgc cagcggcctg cgggccgttc cgccggactg cgacatcgtc
     5881 atggttcacg acgccgtaag gccgtttgtc acgggggaaa tgatcgaccg ggttgcggcc
     5941 gcggcagcct gttgcggcgc cgcttcgctg ggcgtcccgg cgaaagatac gatcaaggaa
     6001 acgaccgatg acggcatcgt cacgagaacg tttccccgga gccgtttgtg gcagacgcag
     6061 acgccccagg cgtttgcctg cgacctgttg cgccgcgcgc atgcgtcggc aaaagaggat
     6121 cactattacg gaacggatga tgcgtcactg gtggaaagaa tcggcgccaa agtccaaatg
     6181 atcgcgggat ccggtgaaaa tatgaaaatt acaacgcccg aagatatgaa gatggcggag
     6241 tccctgctct gcgccggaag aggagagcgg atccgccgcc caagcggatt gggttacgac
     6301 agccatcggt tcgcgaaaga ccggaaacta gtcctgggcg gcgtggacat tccctttgac
     6361 cgggggcttg cggggcactc cgatgccgac gcgcttctgc atgccgtctg tgacgcgctt
     6421 ctgggcatgg ccggtgaagg agacatcggc cggcattttc cggatcacga tcccgcttat
     6481 aaaaatattt ccagcctcat ccttttggaa cgggtcggaa agatggtcga gtccagaggt
     6541 gtgagcattg agaacattga cgcgactctc gtgctggaga agcccaggct ggcgccctac
     6601 gcgcaggcca tggcggccaa catcgcccgt gtactgaaga ttccggaaac ggccgtgaac
     6661 atcaaagcca agaccaacga gggaatgggc tttacgggcc gcggagaggg agtggccgtg
     6721 tgggcggtgg cctcaggcgc ggaaaggatc gcggatggag aataaggcca gagtccgttt
     6781 tgctccgtct cccaccggag agctgcatgt gggcaatgcg cgcaccgctt tgttcaactg
     6841 gatgttcgcc cggcacgaag gcggccgctt cattcttcgg attgaagaca cggatgaatc
     6901 ccgcagcgat tcggcctacc agaccagtct gctggaggac ttgaaatggc tgggcctcac
     6961 ctgggacgaa ggcccggaca tcggcggcgg ctatggccct taccgtcaga gtgagcggct
     7021 ggacctctac gaggcacacc ttaaaaaact ggtggacgcg gacctggcgt acccctgcta
     7081 ctgcacggaa gaagagctgg aagaggagcg ccagacgctg attctcgcca agcgcatgcc
     7141 gcgctacctg gggaaatgcc gcaatctcag cgcaggcgaa cgcgaagcgc tggccgcgtc
     7201 cggccggaag ccttcctgcc gtttcaaggt tccccggaca acgatcgaat ttaatgacct
     7261 gatccgcggg ccgatccggt ttgaaggcga agccatgggc gattttatca tcgtgcggtc
     7321 caacggaatg cccgcctaca actttgccgt agtcatcgac gaccaccgga tggcggtcac
     7381 gcatgtcatc cgcggcgaag accatttgtc caatacggcc ctgcagatca tgctgtatcg
     7441 ggccttagga tttgagccgc cggcttttgc ccaccattgt ctgattctgg gcaaggacca
     7501 cgccaaactc agcaagcgcc acggttccgt atccgtgggc gaattccgca gacagggatt
     7561 tctgcccgaa gcgctgatta attatctcgg gcttctggga agttcctttg ccggaggaag
     7621 ggaaatcctt tcgcgcgacg aaatgatcga tgcgttttct ctctctcggg cgtccagaag
     7681 cggcgcggtc tttgatgaag acaaactccg gtggctcaac gccgcctaca tccgcaaaac
     7741 ctcaacggaa gatctgctca ggaagcttga gccttttttg cgcgccgcgg gctttcggcc
     7801 ggaaacgctt gacgccgcct ggcttaaaaa cgttctggaa accgtgaaag gtgaattgac
     7861 gacgctcgcg gagatcggcg ctcacatcga tctgtttttc gatgaacgct attccctgtc
     7921 tgatgaagct cgaaggctgc tggcggcgcc gggcgcccgc gaggtggtcg gggctttcgg
     7981 cgattatctg gagcgggccg aagacaatgc cggggatatt tacgccgcgg cgatccggca
     8041 cgcaaaggaa aaatccggcg caaaaggaag ggatttgttc atgcccgtcc gggcggcgct
     8101 gaccggaaaa ttgaaaggtc cggaactcga ccgggttttt gccgttctgg gcgtggattc
     8161 cgcccgcggc agatttcagc aggtgaaaaa aacattgggc atctgaaagg gcggcgttcg
     8221 cattcgctgg tttgcctttt attgttttct tcgggttgcc ggatcatccc cgaacccgcc
     8281 tcaaagaaag ccgatcaccc gctgaaacag ttcgtctgcc tcctgagctg atgaaccacg
     8341 gcaaatcata accaatgctg atgacgattt cggggcggat ttaccggagc gtggaagaag
     8401 ggcatggata gcgggaagcg gaattttgat caggatgcgg cgcggtggga tttgaatccc
     8461 gcaagggtaa aactggcctc ggagatcggc agcgctattc ttcgggaaat ttctctgttg
     8521 cctgatagcg atgtccttga tttcggctgc ggaacgggcc tcctcacgct gatgcttcag
     8581 ccgcatgtcc gctccatcac cggcgtggac agctcacaag gcatgctgga cattttaaac
     8641 cggaaaatat ccgaaaggaa tctcaccaat gtcatcaccc gttatgcgga tttggatcag
     8701 ggggatgtcc tttccggggc atttcatctg gcggtcagca gcatgaccct tcaccacatc
     8761 cgggatgtgg cgccgctttt aaaacgcttc tgccatcttc tgcatccttc cggcctcttg
     8821 tgcatcgcgg atctggacga agacggcggg cggtttcaca cctccagcga aggcgtcttc
     8881 catttcggct ttgaccgaca aaagcttgcc cggcagttcg aagaggctgg atttcaaaac
     8941 gtccgcgccg tccaggcggc ttcggttgag aagccggttg cgggcacaat gcagcgcttc
     9001 accgtttttt tgatgatcgg aaggaaatag gcgccgcggg cgtttgccgg ttgccggtca
     9061 ggagaaacgg cagggccgtc atttccgtaa gcgcagacgg cgaaggatgg ctcaggcaac
     9121 gcccccgccc aattcctgtt ggcgttttgg aaaaaaatgt gttcagatgt caagccgaat
     9181 caaggagaaa agcattttga aaaagagcaa gaccagcttc ttctgtcagc actgcggata
     9241 catgtcgccc aagtggctgg gaaagtgtcc gtcctgcagc ggctggaact gttttgccga
     9301 ggagctggtg acggaaccgg attcggtcag ccgcgccgat cgggtgttcg ggggcaagcc
     9361 catgcccatt ttcgatattc ccgccgttga gggagagcga acgtccaccg gcattgttga
     9421 agttgaccgg gtgctgggcg gcggcatcgt cagcggttcg gccatcctca tcggcggcga
     9481 gccgggcatc ggcaaatcga cgctgatgct gcaggtaatg aagaatctgg cgatgggcgg
     9541 gcggaaagtg ctgtacgttt ccggcgagga gtccgcctcc cagatcaagc tgcgagccga
     9601 ccggatcggc gcgcaggcga aggatctcct ggtgctggtg gaagtgtccc tggagaaaat
     9661 cctggagcag attcgaaaaa ccgaaccggc catggtggtg atcgattccg ttcagacggt
     9721 ttattccagc gacctgatgt ccgcgccggg aagcgtcggg caggtgcgtg aagcgtcgtc
     9781 gcgcctgatt ctggccgcca agaaaagctc cattccggtg tttctggtcg gccatgtgac
     9841 aaaagacggc tcgattgccg ggccgaaagt gctggagcac atggtggata cggttttgta
     9901 ttttgagggc gattccgggc acgcctaccg gattgtccga agcatgaaaa accggttcgg
     9961 acccgccaat gaaatcggcg tttttgaaat gcgcgaccgc ggacttcagg aagtgcccaa
    10021 cccctctgcc tttttcctgg ccgaacggcc ggaaaacgct cccggctcgg tggtgctggc
    10081 gagcctcgaa ggcacccgtc cgattcttgt ggagatccag gcgctggtga gccagtccaa
    10141 tttgggcatg cccaggcgaa cggccatcgg ggtggatcac aacagggtgt cgctgctggt
    10201 ggccgtgctg gataaaatct gcggcctgca tctgggcgga tcggatattt tcctgaatgt
    10261 cgcgggaggc gtccgtgtgg aagagccggc cgtggatctg gccgtcgtct cggccatggc
    10321 gtccagtttt ctggaccggc cggtggacgc ccggacggtg attctggggg aagtcggcct
    10381 cacgggcgag gtgcgggccg tctcgcagat tgaaacgcgg gtgaaggaag cggcccgtct
    10441 gggttttaac cggtgcatcg tgcccaaaac caatattgcc tcccttggca aagcggcaaa
    10501 aatggaaatc caaaccatca cttccctgaa ggaactgctg gatatactct tctgaggaca
    10561 tcgtaagaat ttccccaaaa atagtgtccc aatgccttga caggggttaa atcgtgcata
    10621 aaatagcgcc gctaattcga tagtttgcaa tttaatattt acaaggaaga aaggagaaga
    10681 aaccgtatga aaatcagacc cttacaggat cggatcattg taaaacgatt ggaggagg
//
LOCUS       DAOSIM010000047        10737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_6035_length_10737_cov_8.211196, whole genome shotgun sequence.
ACCESSION   DAOSIM010000047 DAOSIM010000000
VERSION     DAOSIM010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 10737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10737
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6035_length_10737_cov_8.211196"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="PLR47_03550"
     CDS             complement(<1..1104)
                     /locus_tag="PLR47_03550"
                     /inference="COORDINATES: protein motif:HMM:NF025022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOG12044.1"
                     /translation="MMNGKKGKAQAEKKRATGEANKTPEKYFVVDTSALEYGIDVFEK
                     LRQGGRNCVLMPYATWEELDSHKTGNDFKADIARSVIRNVRNLFRAQDRSVRFFSYDF
                     TAAKDGGLKTDKNDHCIIATALHARQKLPAEKVILVTQDAALETLALNFGMEVQSVKM
                     DETEVKEQHAPRITVNLQKQWIADKTFPLKLPEARLADKLGQIRLNEGIVCYCERDGA
                     WGPAFAAVRKGDHFRIVDKDIHLLGVRPRSNNGEVNWGQYIAMDLLRDASVPLVALTG
                     KAGTAKTFLSLLAAFEQASRYRRILVSRATIQLGNKDRLGYSPGDIDAKMKPWMHPIY
                     DNISAIKELVDDKMKEKINEWLTNGKIEMMDLDK"
     gene            1346..2935
                     /locus_tag="PLR47_03555"
     CDS             1346..2935
                     /locus_tag="PLR47_03555"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOG12045.1"
                     /translation="MNEKNLILAVDVGTTETKAVLVSPEGYIDSRQSTCCLCYPDKSC
                     VEQSPEEISDAVYHSSREVLAAHPDLAGRLAGVVFTSQMQNTVPVDRNGKPLMNMLSW
                     MDERASCFTQEDMFRGWIKIEGYPVLKLLKFMRITGGAPGKTGKDTVCKLAWMKKNKP
                     DLYASVHKFLDAKDYAIFLATGNYVTSYDMANVTWLMDTRNKDIRKWVWSEEIHKMYG
                     LDSRKMPELRASTAIAGSVTSDFAAKTGVPAGLPVVTGCGDLLASAIGSGAIEMGQLH
                     VNIGTAGWVATHHPGTAIDTQHYVGAIVSGIPGKFLLISKQETLGAALDWLKGMLYPS
                     ELLPGKSTAEIYRMMDAEAALSPSGANGVLLTPWLFGERSPINNPDLRGQIFNISMNT
                     TRADLCRSVMEGVALNIRWGMEYVEKMSLKKSRPTTDEVRLIGGASKSDIWCRIFADV
                     LQKPIVQMKNAQMASAQGAAAVAMVAFGIYRDFSEIDRMLQKGKVFKPNPATAALYEK
                     LYRQYQALYKNNKGAFRELNH"
     gene            2948..4555
                     /locus_tag="PLR47_03560"
     CDS             2948..4555
                     /locus_tag="PLR47_03560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOG12046.1"
                     /translation="MNRFIDSCQDETFDMLVIGGGITGACVAYDAACRGLKVALVEKG
                     DFGAATSAATSKVIHGGLRYLKTFEFGLVRESLKERRVMGNIAPNFIYPTAFMFVNPT
                     FVMKIGLCLYDWIAFDKKFTWDKSKKIPNHYSLSREEVIRQAPNARREGLKGAWVYYD
                     SLSIFPERLTLAFLKSAVHYGAKVSNHARMEGFVMEGGRVSGIVVRDLLNNQIHTLKG
                     KLTVNCAGPWADMVLKMAGKGAAQDSIRRSEGIHLIVRNLVGKQAVGTMTRAGHFFLI
                     PWRNYSLIGTTDKEYPGTPDDYHVTGKSIQELLDTVNRDFGNDNPIRYGDVLYAYGGL
                     RPLLGSGGDIHTITRKYEIYDHADEGLPGLITAEGGKYTTSRNLAQNVLKAAEKRLGK
                     LQPCLTHKKRLVGSEIDDMEGFIEAVKRENRDFSEKTVVYLGRNYGTEYDRVLKMARQ
                     DQALADVLNDDGEILAQAVYAAREEMARTLADVVLRRTGICTLGHPGKEILNKVAAAV
                     ARELKWDGERTEREVEEVEKILKVPAA"
     gene            complement(4571..5638)
                     /locus_tag="PLR47_03565"
     CDS             complement(4571..5638)
                     /locus_tag="PLR47_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12047.1"
                     /translation="MNVDLHNDPDLPQAWAPWFSHEYITYHAVRALDGLTFLPPVAVK
                     PRPPFADKVWPVGPKHIDIYKESYQERRRLDPVWSFDYKKRNGDFALAPPSGKTIEPW
                     KVLVIYSTEPDLYLDHDLAVHKNQKITGGSHGWRHMEFKVLTSTFGAAPKSFLVHQDF
                     SGLAFENGNDYWGWRYLSRCGHYLADLGSPFHVKALPSCFLMKNIFSGQTLFRTASAV
                     HQGYEVYVERRFREGFEPFGQALARGASEAKNAAGNVRHRLPAYMEKARKKHNEVFYF
                     FLDQYGRDLLEVFSRVDPGGPTETASQTNQCSADAARVIFRDGALPALSFLDGITAGI
                     LEDVGLMLGMLFQSFAAAKAR"
     gene            complement(5697..7037)
                     /locus_tag="PLR47_03570"
     CDS             complement(5697..7037)
                     /locus_tag="PLR47_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HOG12048.1"
                     /translation="MAKKKVPADCKEIGPFADKPIALPEGIDMENHVIATYWVQADKN
                     MDMAIMGQFLAIEQTTGTWVPVPGETPEIRAGHVAKVIGVYEAPYYEYGVPPEAANRQ
                     YVLQIAFPAANVEDQLPMMLTAVIGNISLVPNFKLLDLRLPKATLDQYNGPKFGADGW
                     WKALGLKKGRPLLNNMIKPCSGYPLEVGAKLFREAAMGGCDVIKDDELIASMRYNDAV
                     KRVRAYMKIEKEVYEETGEHTLYAVNVTDRLPRMFDLARRCVDAGANSIMVNYLSVGP
                     EAMRALADDPGISVPILGHMDLAGAYFMSPVQGIASPLILGKFARLCGADSVVLPFSL
                     GGKAMYMHDRFMSTVRNLTYPMGGLKPSLPMPSGGITPANVADIVNTLGKDTMIGSGG
                     GIHAHPGGPRAGARALRQAIDAAVRGIGLEEYAKEHEELGVALGVWNKKTDFKI"
     gene            7284..8027
                     /locus_tag="PLR47_03575"
     CDS             7284..8027
                     /locus_tag="PLR47_03575"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOG12049.1"
                     /translation="MQNEDVLARIHLNAVLSNLEILAREDKEASAIAGTWNGGILLMV
                     GLQGPKVTLEMREGAVRVATGRTGNPGIVLLFPTARMLNNLFTGAGRGFPVPVKGLFR
                     IKGLKAFAGLAKRMEAVLKGADAPRKIKARLLLNTVGRTVAVLAASEPESQKAAFGLR
                     GVAELRIKDSEAVHIIFTEQGATAGSGRAPNPDLVMEFSSEEVFLDLTEDKVDVFAAI
                     CLEDIRIQGDLHMGDIVNGLLDQVGVYLQ"
     gene            8038..9273
                     /locus_tag="PLR47_03580"
     CDS             8038..9273
                     /locus_tag="PLR47_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG12050.1"
                     /translation="MTYTFTQSKALFQRAAKVIPQGIYGHFNPATTVPGFYPYFISRA
                     KGCRFWDVDGNEYIDYACAYGPMILGYAHDRIDEAFARQARNGTATMPPSTLLVDLAE
                     KMVEVNDCADWAVFAKNGADAISWAVALARGYRKRDKIVLAHGAYHGTQPWAGTNHIG
                     VLPADRADVLMAPWGDANAFRRVVEENKNRIAGALFTPYHHPVFEPQALPAPGFWRDM
                     RRICDENDIVLIIDDVRAGWRLDIKGSAHYFGFRPDLACYCKAIANGYPISALVGTDR
                     MRMVAAKAFQTGSYWNNSPEMAAALACIDEMQKIDAAAICDSRGGELRRGLEERAAAH
                     GLSFRMSGPNAIPFLHFTNESNFKRAQLFCRECSLRGVYFFWHHNMFLSAAHTEADIQ
                     KTIDVADQAFQIVKDQFGE"
     gene            complement(9392..9847)
                     /locus_tag="PLR47_03585"
     CDS             complement(9392..9847)
                     /locus_tag="PLR47_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOG12051.1"
                     /translation="MTRILIVYHSQTGHTKQMADAVCEGAKAIDDVEVVFKKASEATL
                     DDLLACDGLAVGTPENFGYMSGMLKDFFDRTYVGAQDRVFRKPFVVFISAGNDGSGAL
                     RAIERIALGYKFKTVFAPVIARGNITEEVLDQCRELGGTLAGGCAMGIY"
     gene            complement(9980..>10737)
                     /gene="tpiA"
                     /locus_tag="PLR47_03590"
     CDS             complement(9980..>10737)
                     /gene="tpiA"
                     /locus_tag="PLR47_03590"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOG12052.1"
                     /translation="RILMRKWIVAGNWKMHNTIAESIALARAIKEGFSGSANGEVVVA
                     PPFTALSAVGDALKGSAVRLAAQNMYSEDKGAFTGEISPVMLKDAGCACVIIGHSERR
                     KFFSETDDGVNAKVKKALAAGLKPIVCVGETDEERVQGVTQSVVGRQVRGALAGVEKL
                     DDVVIAYEPVWAIGTGKVATSAQAEEVHAFVRGLIRELFGAAAEELRILYGGSVTKDN
                     VAELAAMEDIDGALVGGASLKPDGFLGIIAKMP"
BASE COUNT         2219 a         3075 c         3189 g         2254 t
ORIGIN      
        1 tttgtccagg tccatcatct cgatttttcc gttggtcagc cactcgttga tcttctcctt
       61 cattttgtcg tccaccagtt ccttgatcgc gctgatgttg tcgtagatcg ggtgcatcca
      121 gggcttcatc ttggcgtcga tgtcgcccgg cgaatacccc agccgatcct tgttgcccag
      181 ctggatggtg gcgcggctga ccagaatccg gcggtagcgg gaggcctgtt caaaggcggc
      241 cagaagcgac agaaaggttt tggcggttcc cgccttgccc gtcagcgcga ccagcgggac
      301 ggatgcgtcg cgcagcagat ccatggcgat atactggccc cagttgacct ccccgttgtt
      361 ggacctgggc cggacgccca gcagatgaat gtccttgtcc acgatgcgga agtggtcgcc
      421 tttgcgcacc gcggcaaagg cgggccccca ggccccgtcg cgctcacagt agcagacgat
      481 gccctcgttg agccggattt ggcccagctt atcggccaga cgggcttcgg gaagtttcag
      541 cgggaacgtt ttgtcggcga tccattgctt ttgaagattc acggtgatcc ggggcgcgtg
      601 ctgctccttg acctccgttt catccatttt gacggattgg acctccatgc cgaaattcag
      661 ggccagcgtt tccagcgccg cgtcctgcgt gacgagaatg actttttcgg ccggcagctt
      721 ttgccgggcg tgaagcgcgg tggcgataat gcagtggtcg ttcttgtcgg ttttcaggcc
      781 tccgtctttg gccgcggtga agtcataaga gaaaaaccgg acggaacggt cttgggcgcg
      841 aaacaggttg cggacattgc gaatgacgct tcgggcaatg tccgctttga aatcattgcc
      901 ggttttgtgg ctgtccagtt cctcccaggt tgcgtaaggc atcaaaacgc agttgcgtcc
      961 tccctggcgc agtttttcaa acacatcgat tccgtattcc agggcgctgg tgtccacgac
     1021 aaaatacttt tccggtgttt tattcgcctc tccggtcgct ctcttcttct ccgcctgggc
     1081 tttgcctttt ttcccgttca tcatgatgtg gttccccgct tgaagagttt gaagttatgg
     1141 aatgcagaat gtgtgaaggc gcacttccaa tgcctgcacc ggctgaattg ttgggtgcca
     1201 ttgtaccaaa ttcggaaatt tcgacaaggg aaagttgaag agggcgcttt ttgggcgaca
     1261 aaaagggctg aactctgcgg caacttgtgg tacaagcatc gcggttcaat catcagtcat
     1321 cttcccggga caaggaggag ccagcatgaa tgaaaagaat ctgatccttg cggtggatgt
     1381 cggcaccacg gaaaccaaag cggtgctggt tagccctgaa gggtacatcg attcccgtca
     1441 gtcaacctgc tgtctttgtt atccggacaa gtcgtgcgtc gagcagtcgc ccgaggaaat
     1501 atcggacgcc gtctatcatt ccagccggga ggttctggcc gcgcatcccg atctggccgg
     1561 aaggcttgcc ggcgtcgttt ttaccagcca gatgcaaaac accgttccgg tggacaggaa
     1621 cgggaagccg ctgatgaaca tgctctcctg gatggatgag cgcgcgtcct gcttcacgca
     1681 ggaagacatg ttccggggat ggatcaagat cgaaggctac cccgttctga agctgctgaa
     1741 gttcatgaga atcaccggcg gcgctcccgg aaaaaccgga aaggatacgg tctgcaagct
     1801 ggcctggatg aaaaaaaaca agccggatct gtatgcttcg gtccataaat ttctggacgc
     1861 gaaggactac gccatttttc tggccaccgg caattatgtg acgtcctatg acatggccaa
     1921 cgtcacctgg ctcatggata cgcgcaataa ggacatcagg aagtgggtct ggtcggaaga
     1981 gattcacaaa atgtacgggc tcgattcccg aaaaatgccg gagctcaggg cgtccaccgc
     2041 tattgccggt tccgtcacct cggattttgc cgcaaaaacc ggcgtcccgg caggccttcc
     2101 cgtcgtgacc ggctgcggcg accttcttgc ctcggcaatc ggctccggcg ccatcgaaat
     2161 ggggcaactg catgtgaata tcggcacggc cggctgggtc gccacgcacc atcccgggac
     2221 ggcgatcgac acgcaacact acgtcggcgc catcgtgtcg ggcattccgg gaaaatttct
     2281 tctgatcagc aagcaggaaa ccctgggcgc ggcgctggac tggctgaagg gcatgctgta
     2341 cccttctgag ctcctgccgg gaaaatctac cgccgagatt tacaggatga tggatgcgga
     2401 agcggccctg tcgccttccg gcgcgaatgg cgttcttcta accccgtggc ttttcggcga
     2461 gcggtccccc atcaacaatc cggacctgcg cgggcagatc ttcaatatca gcatgaacac
     2521 gacgcgggcc gatctctgcc ggtcggtcat ggaaggggtt gccctgaata ttcgctgggg
     2581 catggaatac gtggaaaaaa tgtccctgaa aaagagccgg ccgactacag acgaagtccg
     2641 gctgatcggc ggcgcttcga aaagcgacat ctggtgccgg atttttgcgg acgttttgca
     2701 aaagccgatt gtacagatga aaaacgccca gatggcctcc gcgcagggcg cggccgccgt
     2761 agcgatggtg gctttcggca tttacaggga tttcagcgag attgaccgga tgctgcaaaa
     2821 aggcaaagtc tttaagccga atcccgccac ggccgctctg tacgaaaaac tataccggca
     2881 gtatcaggcg ctgtataaga acaacaaagg cgcttttcgg gaactcaatc attgaggagt
     2941 atgcgccatg aatcgtttca tcgatagctg ccaggacgaa actttcgata tgctggtgat
     3001 cgggggcggc atcacgggcg cctgcgtagc gtacgacgcg gcctgccgcg gcctgaaagt
     3061 cgcgctggtg gaaaaaggcg atttcggcgc ggcgacctcc gccgccacgt ccaaggtgat
     3121 ccacggcggc ctgcggtatc tgaaaacctt tgaattcggg ctggtgcgcg agtcgctcaa
     3181 agagcgcaga gtcatgggaa acatcgcccc gaatttcatc taccccacgg ccttcatgtt
     3241 cgtcaatccg accttcgtca tgaagatcgg cctgtgcctg tatgattgga ttgcatttga
     3301 caagaaattc acctgggaca agagcaagaa aatccccaac cactatagcc tttccaggga
     3361 agaagttatc cggcaggcgc ccaacgccag gcgggaaggg cttaagggcg cctgggtgta
     3421 ttatgacagc ctcagcattt tccccgagcg cctgacgctg gcctttttga aatcggccgt
     3481 tcattacggc gccaaagtct ccaatcacgc ccgaatggaa gggtttgtca tggagggcgg
     3541 cagggtttcc ggcattgtag tgcgggacct gctgaataac caaatccata ccctgaaggg
     3601 gaagctgacc gtcaactgcg ccgggccctg ggcggacatg gtgctgaaaa tggccggaaa
     3661 aggggcggcg caggattcca tccgccgctc ggaggggatt catttgattg tcaggaatct
     3721 ggtgggcaaa caggccgtcg gcacgatgac ccgggccggc catttcttcc tgattccctg
     3781 gcggaactat tcgctgatcg gcacgacgga caaggaatat cccggaacgc cggacgatta
     3841 tcatgtgacc ggtaagagca ttcaggaact gctggacacg gtgaacaggg attttggcaa
     3901 cgacaatccc atccgttacg gggatgtcct ttacgcgtac ggggggcttc ggccactgct
     3961 tggttccggg ggcgatatcc ataccatcac gcggaaatac gagatttacg atcatgcgga
     4021 tgaggggctt cccggcctga tcacggccga gggcggcaaa tacacgacca gccggaatct
     4081 cgcgcaaaat gttttaaaag cggcggaaaa aagactgggc aagcttcagc cgtgccttac
     4141 gcacaaaaag cggctggtcg gcagcgagat cgacgacatg gaaggattca tcgaagccgt
     4201 caagcgggaa aaccgggatt tttcagaaaa aaccgtcgtg tatctcggcc ggaattacgg
     4261 caccgagtac gacagggtgc tgaagatggc ccggcaggat caagccctgg cggatgtttt
     4321 gaatgacgac ggcgagatcc tggcccaagc cgtctatgcc gcccgcgagg aaatggcccg
     4381 aacgctggcc gacgtggttt tgcgcagaac gggcatttgc acgctgggcc atccggggaa
     4441 ggagatccta aacaaggtgg ccgccgcagt tgcccgggag ctgaagtggg acggagaaag
     4501 aacggagcgc gaggtcgaag aggtggaaaa aatcctgaag gttccggcgg catgacgctg
     4561 tgtgaacgat ttacctcgct ttggccgccg caaaggattg aaacagcatt cccagcataa
     4621 ggccgacgtc ctccagaatc ccggcagtga tcccgtcgag gaaggaaagc gccggaagcg
     4681 cgccgtctct gaaaatcacc ctggccgcgt cggcggagca ttgattggtc tgtgaggccg
     4741 tctctgtggg gccgccggga tctacccggc tgaaaacctc cagcaggtcc cggccgtact
     4801 gatcgaggaa aaaataaaaa acctcgttgt gcttttttct tgccttttcc atgtaagccg
     4861 gaaggcgatg ccgcacattt cctgccgcat ttttcgcttc gctcgctccc cgcgcaagag
     4921 cttgcccgaa cggctcaaac ccttcacgga agcggcgttc aacatacacc tcatagccct
     4981 gatgcaccgc ggaagccgtc cggaacagcg tctgtccgga gaagatgttt ttcatgagga
     5041 aacaagaggg cagcgccttc acgtgaaagg ggctgcccag atccgccagg taatggccgc
     5101 accgggacag gtagcgccac ccccagtagt cgttgccgtt ttcaaaagca agcccgctga
     5161 aatcctgatg aacgaggaag gatttgggcg ccgcgccgaa ggtcgaggtc agcaccttga
     5221 attccatgtg gcgccagccg tgggacccgc cagtgatttt ctgatttttg tggacggcca
     5281 gatcgtggtc cagataaaga tccggctccg tgctgtaaat caccagcact ttccaaggct
     5341 cgatcgtttt ccccgaaggc ggcgccaggg caaaatcgcc gtttcgtttc ttgtagtcga
     5401 aagaccatac cggatccagt cgccgtcgct cctgatagct ttccttgtaa atatcgatgt
     5461 gcttcggacc gacgggccag accttgtcgg cgaaaggggg acggggcttc acggcgacgg
     5521 gcggcaggaa ggtgaggccg tccagcgccc ggacggcgtg gtacgtgatg tattcgtggg
     5581 agaaccaggg cgcccaggct tggggcaaat ccggatcatt gtgaagatcg acattcaagg
     5641 ccgggcatcc ttaagcgatg cggggaaagg ccggaacctc tccccgcaaa ccatagctag
     5701 attttgaaat cggttttttt gttccagacg cccagcgcga cgcccagctc ttcgtgttct
     5761 ttggcgtatt cttccagccc gataccccga accgccgcgt cgatggcctg acggagcgca
     5821 cgggcgccgg ccctcgggcc gcccggatgg gcgtgaatgc cgccgcccga gccgatcatt
     5881 gtgtctttgc ccagcgtgtt gacgatgtcc gccacgttgg ccggcgtgat gccgcccgac
     5941 ggcatgggca gagagggctt caggccgccc atcggatacg tcagattgcg cacggtgctc
     6001 atgaaacgat cgtgcatgta catggccttg ccgcccagag agaagggcag aacgaccgag
     6061 tctgctccgc acagacgggc aaatttgcct aaaatgagcg gcgaggcgat tccctgaacg
     6121 ggcgacatga agtaggcgcc ggccaggtcc atgtggccca ggatcggaac ggaaatgccc
     6181 ggatcatcgg ccagggcgcg catggcctcc ggaccgacgg acagataatt taccatgatc
     6241 gagttcgcgc ccgcgtcgac gcagcgcctg gccaggtcga acatccgcgg caggcggtcg
     6301 gtgacattga cggcgtaaag ggtgtgctcg ccggtttcct catacacttc cttttcgatt
     6361 ttcatatagg ccctgacgcg tttgaccgcg tcgttgtagc gcatgctggc gatcagctcg
     6421 tcgtccttga tcacgtcgca gcctcccatt gccgcttccc ggaagagctt ggcgccgact
     6481 tccagcggat agccgctgca gggcttgatc atgttgttga gcagcggacg gccctttttg
     6541 agccccagcg ccttccacca tccgtccgcg ccgaatttag gcccgttgta ctggtccagc
     6601 gtggctttcg gtagccgaag gtccaggagt ttgaagttgg gaaccagcga gatgttgccg
     6661 atgacggcgg tcagcatcat gggaagctgg tcttcgacgt tggcggcggg aaaggcgatc
     6721 tgcaggacgt actgccggtt tgccgcctcc ggcggaacgc cgtattcgta gtagggcgct
     6781 tcatagacgc cgatcacttt tgcgacatgt ccggccctga tttccggcgt ttcgccggga
     6841 accggcaccc aggtgccggt ggtctgttcg atggccagaa actgccccat gatcgccatg
     6901 tccatgtttt tgtcggcctg tacccagtaa gtggcaatca cgtggttttc catatcaatg
     6961 ccttcgggca gggcgatggg tttgtccgcg aagggaccga tctctttgca atcggcgggg
     7021 actttctttt tcgccatggg atactcctct cggtgaattt tattttttga cggcgcggat
     7081 gtcgtctgcg tccgcggcag acgacgggat gagccgccgg gaaacggacc ggccgatcaa
     7141 ccggacggca ccggttttcc gggttttcta tcgcacagat cccgaaatag ttcaagcgcc
     7201 tatccggtat tgacagggta tcccgggaag agtaaaaagg aaaacacacg tcatgcttca
     7261 ttggcaaaac cggggaggga tagatgcaaa acgaagatgt tttggcaaga atccacctga
     7321 atgcggtgct ttccaatctt gaaatccttg cacgggaaga caaggaagca tcggcgatcg
     7381 ccgggacgtg gaacggcggc atcctgctca tggtcgggct tcaaggcccc aaggtcacgc
     7441 tggaaatgag agagggtgcc gtgagggttg cgactggccg aaccggaaat cccggcatcg
     7501 ttttgctttt tcccaccgct cgaatgctca acaatctgtt taccggcgcc ggccgcggct
     7561 ttcctgttcc cgtaaaaggt ctgttcagga tcaagggact caaagcgttt gccggacttg
     7621 ccaaaaggat ggaggccgtt ctcaaaggcg ccgacgctcc ccggaaaatc aaagccaggc
     7681 tgctgctcaa cacggtgggc aggacggttg ccgttcttgc cgcatcggaa ccggaatccc
     7741 aaaaagccgc cttcggactt cggggggttg cggagcttcg aatcaaagac agcgaagcgg
     7801 ttcacatcat ctttaccgaa cagggtgcga ccgcaggaag cggccgcgcg ccgaatcccg
     7861 accttgtcat ggaattttcc tcagaggagg tgtttcttga cctcacggaa gacaaggtgg
     7921 atgtgtttgc cgccatctgc ctggaagaca ttcgaatcca gggggatctg cacatggggg
     7981 atatcgtgaa tgggctcctc gaccaggtcg gtgtttatct gcaataggag acagggcatg
     8041 acctatacct tcacgcaatc caaagcgctt tttcagcgcg cggccaaagt cattccccag
     8101 ggcatctacg gccatttcaa tccggccacg acggttccgg gcttctatcc ctacttcatc
     8161 tccagggcca aggggtgccg cttctgggac gtggacggca atgaatacat cgactatgcc
     8221 tgcgcctacg gtcccatgat cctcggctac gcccatgacc ggattgacga agcctttgcc
     8281 cgtcaggccc gaaacggaac ggcgaccatg ccgccttcga cgctgcttgt cgatctggcg
     8341 gaaaaaatgg tggaggtcaa tgactgcgcc gactgggccg tgtttgccaa aaacggcgcg
     8401 gatgcgattt cctgggccgt ggcgcttgcg cgcggctacc gaaagcgcga caagatcgtg
     8461 ctggcccacg gggcgtatca cggaacccag ccgtgggcgg gcaccaacca catcggcgtt
     8521 cttccggccg accgggcgga cgtcctcatg gcgccgtggg gcgatgcgaa cgcctttcgg
     8581 cgggtcgtgg aggaaaacaa aaaccggatc gccggcgccc tgttcacccc ctatcatcat
     8641 cccgtctttg agccgcaggc gcttccggcg cccggattct ggcgggacat gcgccggatt
     8701 tgcgatgaga atgacatcgt cctgatcatc gatgacgtcc gcgccggatg gaggctcgac
     8761 atcaaaggct ccgcccatta tttcggcttc cggcccgatc tggcctgtta ctgcaaggcg
     8821 atcgccaacg gctatcccat ctccgccctg gtcggcacgg accggatgcg catggtcgcc
     8881 gcgaaagctt ttcagaccgg ctcctactgg aacaattctc cggaaatggc ggcggccctg
     8941 gcctgcatcg acgaaatgca aaaaatcgac gccgccgcga tctgcgactc ccgcggcggc
     9001 gagcttcgcc ggggactgga ggaacgagcc gcggcgcacg gcctttcgtt tcgaatgtcc
     9061 ggaccgaacg ccatcccctt tctgcatttt accaatgaaa gcaacttcaa gcgcgcccag
     9121 ctcttctgca gggaatgttc actgcgaggc gtttacttct tctggcatca caacatgttt
     9181 ttgagcgcgg ctcataccga agcggatatt cagaaaacca ttgatgtggc cgaccaggcc
     9241 tttcagatcg tcaaagatca attcggagaa taaccgtcag aatccatcgt gtgatgatgt
     9301 tttgggcagc gacaaaaccc gccttcggct caaaccgttg tcatgactga tctttcgcct
     9361 atggcctatt gcccgtgacc cattgcctca tttaatagat gcccatcgcg cagccgccgg
     9421 ccagcgtgcc tcccagctcg cggcactggt cgagcacttc ttcggtgatg ttcccgcggg
     9481 cgataacggg cgcgaagacg gtcttgaatt tatagcccag agcgatgcgt tcgatggccc
     9541 tgagcgcgcc cgagccgtcg ttcccggcgc tgatgaagac gacaaagggt ttgcggaaaa
     9601 ccctgtcctg cgcgccgaca tacgtgcggt cgaagaaatc cttgagcatg cccgacatgt
     9661 agccgaagtt ttccggcgtg ccgacggcca ggccgtcgca ggccagaagg tcgtctagcg
     9721 tggcctcgga ggcttttttg aaaaccacct cgacgtcgtc aatggccttc gctccttcgc
     9781 aaacggcgtc tgccatttgt ttggtgtgtc ccgtctggga atggtaaacg attaagattc
     9841 tggtcatgat aaaaggcaat gggttatggg tgataggtga taggttatgg gtgatgggtt
     9901 aggggagaaa agcggcgaaa ggacttctgt ttcccggtcc tctcgcctct tacctctcgc
     9961 ccattaccca taacctcttt tacggcattt tggcgatgat gcccagaaag ccgtccggtt
    10021 tgagcgatgc gccgccgacg agcgctccgt cgatgtcttc catagcggcc agttccgcga
    10081 cattgtcctt cgtcacgctg ccgccgtaaa gaatgcggag ctcttcggcc gccgccccga
    10141 acaattcccg aatcagaccg cggacgaaag cgtgcacctc ctcggcctgc gcggaagtgg
    10201 cgactttgcc cgtgccgata gcccagaccg gctcgtaggc gatcaccaca tcgtccagct
    10261 tttcaacacc ggccagagcg ccccgcacct ggcggccgac aaccgactgc gtcacgccct
    10321 gaactctttc ctcatccgtt tcacccacgc agacgatcgg cttgagcccc gcggcgagcg
    10381 cctttttcac tttggcgttg acgccgtcat ccgtttcgga aaaaaacttg cgccgttccg
    10441 aatggccgat gatgacgcaa gcgcagccgg catccttgag catgacgggg gaaatttcgc
    10501 ccgtgaaggc gcccttgtcc tcgctgtaca tgttttgcgc ggccagccgc accgcagaac
    10561 ctttaagtgc gtcgcccaca gcggaaaggg cggtaaacgg cggcgccacc acgacttcgc
    10621 cgtttgccga gccggaaaat ccttccttaa tcgcgcgggc cagggcgatc gattcagcga
    10681 ttgtgttgtg catcttccag ttgcctgcga caatccattt tctcatgagg atcctcc
//
LOCUS       DAOSIM010000048        10500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_6196_length_10500_cov_4.907037, whole genome shotgun sequence.
ACCESSION   DAOSIM010000048 DAOSIM010000000
VERSION     DAOSIM010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 10500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10500
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6196_length_10500_cov_4.907037"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..913
                     /locus_tag="PLR47_03595"
     CDS             218..913
                     /locus_tag="PLR47_03595"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOG12053.1"
                     /translation="MLASDDTQSDAWRRDLIQDYLTRDIPSLGFALPGPVLSRFWKMI
                     AHYHGGIFNASKIGQSMDISHNTVRKYLDILEQTFMVRLLQPLEINLKKRLVKSPKIY
                     LRDSGLLHTLLEIDSATELYGHPVFGASWEGWCIEQIIATLPFWQPFFYRTSSGEEID
                     LVLVKGKKRLIFEIKASLTPHLSKGFADTMKALTPERVWVVCPMTDSGYPIQSDGRVT
                     GISECLGDLAVYE"
     gene            complement(1039..7311)
                     /locus_tag="PLR47_03600"
     CDS             complement(1039..7311)
                     /locus_tag="PLR47_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12054.1"
                     /translation="MLLAFCAAFALALSPPVGTVIPNTATARFTGANGYAFPDVPSNR
                     VETAIGGAPVLAITAVATPDPTAPGGTLTYMITVSNTGNLPASAVTVAATLSPILQFQ
                     GASNGGISSPPLVTWNLGAIGPGGGVTFTLTAVVAAGTLPGTVIPFTASTTSAEGAAN
                     SATLTTTVGAGSNLVFEKSGAPETVAPNGVITYALSYRNIGNQTANGVRIVDSIPSGT
                     AYVAGSASAFGSLTGSVISWNIGDVAAGGAGEVSFRVRVSPVAVSGQQISNTASILST
                     AQTKTSNTVVTNVSTPSLLLLKLDTPDPVRAGNNIVYTLQVENTGSIPLTGIVLSDPI
                     PVGTTFVSAGNGGVAAAGARQVEWAIGALAAGAKQTVTLTVQVDKALVQGQVIENTAT
                     ASSNEMPPRTVRAVSVVSARTQGTIEFFDAAWQPAYAYRSGNAVNLQVLDADQNLNPL
                     VAETVTVVLTDSKTGDTETVIMTETGPDTGIFRGGVTSSLAASAANSGILTVSANSRV
                     TATYSDALDAVVVSTASALIDPLGIVFNSVTGAPVAGTVVTLRNWNGVANSCDLTSWP
                     VLPPGEVNPAVPTGADGKFAFPLVPPGDFCFQVTPSAGYTFPSAVAASDLPAGFTIGN
                     GSRGEKFTLNVGDPPLIRDIPVDPPTGRLTIAKTASKTTAAIGDMLTYVLNLTNNGSS
                     PVNSLTITDIMPHGLAYIAQSTKTNGVASADPQTAGKRTFAWTIASLAPAASVEIAYR
                     VLVGPDSQAGTGINTATAAGLSLGKTVVSNTASVKVKITEGVFTSKGTIIGRVFLDLD
                     ENRLAGKDSGIPDVALYLEDGTRVVTDKRGRFSISGVSAGTHVLRLDETTLPAGAKLI
                     PLNNRFMRDGASQFVDMTPGGLFKANFAVQKGSMILKAPAPEAQKEKQEQENTEQQGR
                     TLVEALSDIFSSVPASRKAVSSKASPAGAATVEGPPVKKPEEMKASVSTRVASDPQEE
                     AKASSAMPDAAATKEAPAVKTSAEPKTDQTPPAESRPAAKEENEADAGAEKVSAEKEA
                     GLEERIKTFSPDLEFLNLRDQEVAARKNIRVVVKAPLDTELTLTVNGDKVDNRQIGKQ
                     IKYAPGKVAIYEFINIPLKTGEENLITAEVRDPFGNVRSSQTIRIKAAGDAARMVIRP
                     ERTEAPADGQSKISVNVSLEDREGQIATFSGSAAVSLSAGEILEKDADPLADGHQILI
                     RNGVAQFTVVSPRETGEAKIEIQVNDVSESVKIYFLPHLRPMLMVGVGEVVLGNGRSS
                     GDLDYIKSRSWFNDGAYVNGRGAFFMKGNVFKDFLLTASYDSNKNKTDELFRESDTRL
                     DSEEKYPIYGDESKTGYEALSREKLYVKLEKGKSYLLYGDYRTDLTETTLAAYTRSFN
                     GLSAEVNTDRFRLRSFGTHTDQSQFVDTLPGKGISGYYYLNNNTIIEGSERVVIETRD
                     RLQPDRVLNREVKSNGSDYDIDYAMGTILFKAPVPSHDADGNPVYIVATYESRQEGSK
                     YYIYGGRGAFKVTDWLQVGATGVIEENAISNYQLFGGDIALKLPGKTLVKAEYVNTRG
                     LFDINNTYAAKDGEGWSVNVESQPLNRLSVNGFYRNMSDYFSNPSATDAVRGTQKFGF
                     DAAYEIQPSLTLRAKYLDEQDRINNSTHRLASVGATKKFVKTKISAELTHETSDNLTV
                     TPAQTPFTPGGLLNGVPFLNAYETPDQATFARVELERELLTDLSLTLSHKQDLGGNSY
                     FVSQGGLNYKINEFNRLYLREEYAKYQQGTQTRTLIGAESSLVKNTTAYSEFRLADGS
                     AGYRNQQVIGLKNKFQIIRGLNANIAGEYLSTLSGQKNPNEPDAYAAAASMDYLAKED
                     LKITGRAEHRHEIVENGTDSYLAEVAAAYKLHPDFSLLVRERYFIEKKGGDQNDTNRL
                     LLGLAYRPLENDRFNALGKVEYKYEKRGTAQPAYGTDSFIASAEGVYQLTQTMQVMGK
                     YAGKLEKDEDFSSYTDLMAARIMIDLTDRFDFGAEYRLLTSHKTNTRQHGGSVEFGCR
                     IVDQIWLSLGYSFDRFDEDLAGDSYHGEGPYLKVRFKFDEKTLRKLNPLADKNSSSAP
                     QAAASIEKALSSYLSK"
     gene            complement(7469..9193)
                     /locus_tag="PLR47_03605"
     CDS             complement(7469..9193)
                     /locus_tag="PLR47_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12055.1"
                     /translation="MKTVKAPLKFVVLLIAGLLLSTMLSAGTAFAAGTPAGTTITNKA
                     TATYTDANNNTYAPVESNTVSVTVSQVAGVTTSPATVSQNGSPGGKTYFSGQIVNSGN
                     GTDTFNLTAAGLPAGYTYAIYKDTNGDGILQPSEEVAGNMLGASVSLDADQVQYAIAV
                     ITAPAGAAAGSTASLNFTATSVLTPAVSSTSTMTVTIQAAVITFSKSATPAAPKPGET
                     VTYSITWNNSGTAPGYLVVLTDAIPAETTYKTGSMTYSGAARTDAADADNADYNVTNP
                     GKVTAAIGTVAAGGSGTFSFQVTVNANIAASTVINNTASATYRTNAGDPGTETTVNTN
                     TSPFTVSQAAGVQVMPPTLTTDELVGNQNLHPFTIKNTGNSTDIYSISSVGLYWTWAV
                     YNDVNQDGKYTAGVDTPVTDSNADGKIDTGAVAPDETKFYVAVVTVAGSNGQQGKHTI
                     TATSVYDTTVTGTSIKFTNIQTPVMGLVKSVSPAGPQPPGTQLTYTITVTNTGAAPAQ
                     TFVVTDVLSSYLNYVPNSITVAGAGQTDAADGDFGHYDAVSGTVMISIPSIAAGATIP
                     IVYRATIK"
     gene            complement(9242..9760)
                     /locus_tag="PLR47_03610"
     CDS             complement(9242..9760)
                     /locus_tag="PLR47_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12056.1"
                     /translation="MTNVKTFRWMVLWGALFLAALLLAAPQARADEPRIALKVSGEKE
                     VTQIRNGKKTVTTAPLDKIKSGDILVYTVMYHNTGKSVARDVTVVDPVPANTVYLPGS
                     AAGKDAKIFFSINGGATFVEPPVLRKSLDAAGKEILQPAPPELFTHIKWIIKAPVPAN
                     ATGTVTFKVKVK"
     gene            complement(9858..>10500)
                     /locus_tag="PLR47_03615"
     CDS             complement(9858..>10500)
                     /locus_tag="PLR47_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12057.1"
                     /translation="TVTNNGPSRVESLTVIDTLPPLLQSPVFKPSQGIYNEATGLWTG
                     LNLMPGQSIALSLTVLVDPAVAGSFTNTATVSPPAGVTDPVPGNNSADDTDTTMPHIT
                     LTKNVDPSTATPGQDVLFTIYYRNVGGSAASNLIIFDTVPPNTTYVTGSLKSGNAAST
                     YATANSLTDIADADVGQINGENVTFIISTVAGDDGADNAGNDEGKVYFKVRIR"
BASE COUNT         2001 a         2946 c         3085 g         2468 t
ORIGIN      
        1 cttcagccgt tatgtgaatg tttacgaagg catggcgctg gccgtccaga aaaacgccat
       61 cctgctcatg tcggaagact tgaaagaagc catgatgtct tttctggaaa gacgcccggc
      121 ggatttcaag ggaaaatgat cttgatgagc ccttcgggtt tcggccaacc ggcttttgag
      181 cccgaagggc tgtcttgaga tcatcagtcc atcgtctttg cttgcatccg atgacaccca
      241 aagcgacgcc tggcgaaggg atttgattca ggattattta acccgcgaca ttccgtcgct
      301 gggatttgcg cttcccggtc ctgttctgtc gcgcttttgg aaaatgatcg cgcactatca
      361 tggcggaatc tttaacgcat cgaaaatcgg ccaatcgatg gacatctcgc acaacacagt
      421 ccgtaaatac cttgatattc ttgaacaaac gtttatggtg cgccttctgc aacctctgga
      481 gatcaatctt aaaaaacgcc tggtcaaatc gcctaagata tatctccgcg acagcgggct
      541 tttgcataca cttctggaga ttgacagcgc cacggaactt tacggccatc cggtattcgg
      601 ggcatcctgg gagggctggt gtattgaaca aatcattgcg accctgccgt tctggcaacc
      661 gtttttttac agaacgtctt ccggtgaaga aattgatctg gttttagtca aagggaaaaa
      721 gcgcctgatc tttgaaatca aagcctctct gacgccccat ttgtccaaag gctttgccga
      781 tacgatgaaa gcgctcacgc ccgagcgcgt ctgggttgtc tgccccatga ctgattccgg
      841 ctacccgatc caaagcgacg gacgcgtaac gggaatcagc gagtgcctcg gtgatctggc
      901 tgtctatgaa taatcggcga gagttatgcc cagaaatcat agggagaaca aggggttgaa
      961 accccttgtt ccagcttgca gggaacggtc gcgaccgttc cctgctttat ttccgtaaac
     1021 gggcgacgcc gaaattcatc atttgctcag ataagatgaa agcgcttttt caatcgatgc
     1081 ggcggcctgc ggcgccgaag aagaattctt gtcggccagc gggttcagct ttcgcagcgt
     1141 tttttcatca aacttgaagc ggaccttcag gtacggacct tcgccgtggt aactgtcgcc
     1201 cgccagatcc tcgtcaaacc gatcgaaaga gtaacccagg gaaagccaga tctgatcgac
     1261 gatgcggcag ccgaattcga ccgaaccgcc gtgctggcgg gtgttcgtct tgtggctggt
     1321 gagcaggcgg tactccgcgc cgaaatcaaa ccgatcggtg aggtcgatca taattcgggc
     1381 tgccatcagg tctgtatagg aagaaaagtc ttcgtctttt tcgagttttc ccgcatattt
     1441 gcccatcacc tgcatggttt gcgtcagctg ataaacgcct tccgcggagg cgatgaagga
     1501 atccgttcca tacgcgggct gggcggttcc ccgtttttcg tatttatact ccaccttgcc
     1561 cagggcgttg aagcggtcat tttcaagagg gcgatacgcc aggcccagaa ggagccgatt
     1621 ggtatcgttc tgatcgccgc ccttcttctc gataaaatat ctttcccgca ccagcaaaga
     1681 gaaatccgga tgcagcttgt aggccgcggc cacttccgcg agatacgaat ccgttccgtt
     1741 ttccacgatt tcatgccggt gttcggcgcg gcctgtgatt ttcaggtctt ccttggccag
     1801 gtagtccatg ctggccgcag ccgcgtaggc atccggttcg ttcggattct tctgaccgct
     1861 gagggtgctc agatactccc cggcgatgtt ggcgttgagc ccccggataa tctggaattt
     1921 gttcttcaat ccgatcacct gctggttgcg gtatccggct gatccgtcgg ccaggcggaa
     1981 ttccgagtag gccgtcgtgt tcttgacgag cgacgattcc gcgccgatca gcgtgcgggt
     2041 ctgggttccc tgctggtatt tcgcgtactc ctccctcaga taaagccggt tgaactcgtt
     2101 gatcttgtaa ttcaaaccgc cctggctgac gaaataggaa ttgcctccca gatcctgctt
     2161 gtgactcagt gtcagggaca aatcggtcaa aagctccctt tccagttcca ccctggcaaa
     2221 ggtcgcctgg tccggcgttt cgtaagcgtt gaggaaggga acaccgttga gcagcccgcc
     2281 cggggtaaaa ggtgtctggg ccggtgtgac ggtaaggtta tcggatgttt catgggtcag
     2341 ctcggcgctg attttggttt tgacgaattt tttcgtcgcg ccgaccgacg ccagccggtg
     2401 cgtcgagttg ttgatcctgt cctgttcgtc caggtacttg gcccgcagcg tcagggacgg
     2461 ctggatttca taggcggcgt cgaagccgaa cttctgcgtg ccgcgcaccg cgtccgtcgc
     2521 cgagggattg ctgaagtagt cgctcatatt gcggtaaaaa ccgttgaccg acaggcgatt
     2581 caggggttgg ctctccacgt tcaccgacca gccttcgccg tctttggccg catacgtgtt
     2641 gttgatgtcg aacagcccgc gagtattgac atattccgcc ttgaccagcg ttttgccggg
     2701 cagcttcaag gcgatatcgc cgccgaagag ctgataattg ctgatcgcgt tttcctcgat
     2761 gacacccgtg gcccccacct gcagccagtc cgtcacttta aaagcgccgc ggccgccgta
     2821 gatgtaatac ttgctgccct cctgccggct ctcgtaggtg gccacaatgt aaacggggtt
     2881 gccgtccgcg tcatggctgg gaaccggggc tttaaacaga atggttccca ttgcgtaatc
     2941 gatgtcgtag tccgatccgt tcgatttgac ttcccggttc agaacgcggt caggctgcag
     3001 ccggtcgcgg gtttcgatga ccaccctttc cgatccttcg atgatcgtat tattgttgag
     3061 atagtagtat ccggaaatcc ctttgccggg cagggtatcg acaaactggc tctggtcggt
     3121 gtgcgtgccg aaagagcgca ggcggaaccg gtcggtgttg acttcggccg aaagcccgtt
     3181 gaaggatcgc gtgtaggcgg caagcgtcgt ctccgtcagg tcggtccggt aatcgccgta
     3241 gagcaaatag gatttgcctt tttccagctt gacgtagagc ttctcgcggg aaagagcctc
     3301 gtagcccgtt ttgctctcat cgccgtaaat gggatacttt tcttccgaat ccagccgcgt
     3361 gtccgattca cggaacagct catcggtctt gttcttgttc gagtcataag aggccgtcag
     3421 caggaaatcc ttaaaaacat ttcccttcat gaagaaagcg ccgcgtccgt tcacatacgc
     3481 gccgtcattg aaccaggacc ggctcttgat gtaatcgaga tcgccggatg accggccgtt
     3541 gcccagcaca acctcgccga cgccgaccat cagcatgggc cggaggtgcg gaagaaaata
     3601 gatctttacg gattcgctga catcattcac ctgaatctcg atttttgctt cgcccgtttc
     3661 ccggggagag acgactgtaa attgagcgac gccgttccgg atcagaatct gatggccatc
     3721 cgccaacgga tcggcgtcct tctccagaat ctcgccggcg gaaagcgaca ccgcggctga
     3781 tccgctgaag gttgcaattt gaccttcacg atcttccagc gatacgttga cgctgatctt
     3841 ggattgtcca tccgccgggg cttcggtgcg ctcgggacgg atgaccatcc gcgccgcatc
     3901 gcccgccgcc ttgatccgga ttgtctgtga gctccggaca ttgccgaaag gatcacgaac
     3961 ttcggcggta atcaggtttt cttccccggt cttgagcgga atgttaataa attcataaat
     4021 ggccactttg ccgggagcat atttgatctg tttgccgatt tgccggttgt ccaccttatc
     4081 gccgttgacg gtcagggtga gttccgtatc caggggcgct ttgacgacca cgcgaatgtt
     4141 tttccgcgcc gcgacctcct gatcccgaag attcagaaac tccaggtccg gtgaaaaagt
     4201 cttgattctc tcttcgagcc cggcttcttt ctctgcagaa actttttccg caccggcgtc
     4261 cgcttcgttt tcttcctttg ctgcgggccg ggactcggcc ggaggcgtct ggtctgtttt
     4321 cggttcggcg gatgtcttta ccgccggcgc ttctttggtt gcagccgcat caggcattgc
     4381 cgacgacgcc ttcgcttctt cctgcgggtc ggaggcaacc cttgttgaaa cggatgcctt
     4441 catttcctct ggctttttga caggcggtcc ctcaacggtt gccgcgcccg caggcgatgc
     4501 ttttgaggag acggcttttc ggcttgcggg aactgaagaa aaaatatccg agagcgcctc
     4561 cacaagcgtt ctgccctgct gttccgtatt ttcctgttcc tgcttttcct tctgcgcttc
     4621 tggggcaggc gccttcaaga tcatggagcc tttttgaacc gcaaaattcg ccttgaagag
     4681 accgccgggc gtcatatcga caaactgcga agcgccgtcg cgcataaaac ggttattaag
     4741 cgggatcagc tttgcgcctg caggcagcgt ggtctcgtca agccgcagca catgggttcc
     4801 cgccgagact ccggaaatgg agaagcggcc gcgcttgtcc gtgaccactc ttgtgccgtc
     4861 ttccaggtag agcgccacgt cgggaattcc cgaatccttt ccggccagcc ggttttcgtc
     4921 aaggtccagg aaaaccctgc cgatgatggt tcctttcgac gtgaagacgc cttcggtgat
     4981 tttcactttg acgctggccg tgttggaaac gaccgtcttg cccaggctca agccggccgc
     5041 cgtcgccgta ttaatccctg ttcccgcctg actgtccggc cccaccagca cgcgataggc
     5101 aatctccacc gacgcggcgg gcgccaggct ggcaatggtc caggcaaagg tccgcttgcc
     5161 cgccgtttgc ggatcggcgg atgccacgcc gttcgttttg gtcgattgcg caatgtaggc
     5221 caggccgtga ggcatgatgt ccgtaattgt aagactgttt accggcgagg atccgttgtt
     5281 cgtcagattg aggacataag ttagcatatc cccgatcgcc gccgtcgttt tgcttgccgt
     5341 cttggcgatg gtcaagcggc ctgtgggggg gtccaccgga atatcgcgga tgagaggagg
     5401 atcacccacg ttcaaggtga acttttcacc ccgtgagccg ttgccgatcg taaagccggc
     5461 aggcagatcg ctcgcggcaa ccgccgaggg gaaggtgtaa cccgcggaag gcgtcacctg
     5521 gaagcagaag tctcccggcg gaaccagcgg aaaggcaaac ttgccgtccg cgcccgtggg
     5581 aacggccgga ttcacttctc ccggcggcag aaccggccag ctggtcaggt cgcaggaatt
     5641 ggcaacgccg ttccagttgc gcagcgtcac cacggttccc gccacgggag cacccgtgac
     5701 ggaattgaaa acgatgccca gcggatcgat cagggccgat gccgtggaaa cgactacggc
     5761 atcgagcgca tccgagtagg ttgccgttac ccgggagttt gccgacaccg tcaagattcc
     5821 gctattggcg gcggatgcgg ccagagaact ggtcacgccg ccccggaaga tgcccgtatc
     5881 cggccccgtt tccgtcatga taacggtttc cgtgtcgccg gtttttgaat ccgtcaatac
     5941 caccgtgacg gtttcggcga ccagcggatt cagattctga tccgcgtcca gaacctgaag
     6001 attgacggca ttgccgcttc gataggcata ggccggctgc caggctgcgt caaagaattc
     6061 aatcgtgccc tgggttctcg cgctgaccac ggagactgca cggaccgtcc gggggggcat
     6121 ttcattggag gacgccgtgg cggtgttttc gatcacctgg ccctggacca gggctttgtc
     6181 cacctgcacc gtcagggtga ccgtttgttt cgcgcccgcc gccagcgctc cgatggccca
     6241 ttccacctgt ctcgcgccgg ccgcggcaac gccgccgttg ccggctgata caaaggttgt
     6301 ccctacggga atcggatcgc taaggacgat gccggtcagg ggaatgctgc ccgtgttttc
     6361 cacctgcagg gtatagacga tgttgtttcc ggcgcggaca gggtcgggcg tgtccaactt
     6421 caaaagcagc agggacgggg tcgagacgtt cgtgaccacc gtgttggagg tctttgtctg
     6481 cgcggtgctg agaatggaag ccgtgttgct gatttgctga ccggaaacag ccaccggaga
     6541 aacacgaacc cggaaggaaa cctcgcccgc gccgcccgcc gcgacatcgc cgatattcca
     6601 ggaaatcacg cttcccgtca aactgccgaa cgcgctcgcc gatccggcaa cataagccgt
     6661 tccggaggga atagagtcca caatccgaac gccgtttgcc gtctggttgc cgatgttgcg
     6721 gtaggaaaga gcataggtga tcacgccgtt gggcgcaacc gtttcgggcg cgccggattt
     6781 ttcaaaaacc agattgcttc ctgcgccgac ggtcgtggtc agcgtcgccg aattggcagc
     6841 cccttcggcg gatgtcgtgg aagcggtgaa cggaatcacg gttcccggaa gcgttccggc
     6901 cgcgacgacg gccgtaaggg tgaaggtcac gccgccgccc ggcccaatgg cgcccagatt
     6961 ccacgtcacg agcgggggtg aggatattcc tccgtttgac gccccctgaa actggagaat
     7021 cggagaaagg gtcgcggcaa ccgtcacagc ggacgccgga aggttgcccg tgttggaaac
     7081 ggtgatcata tatgtgagcg tgccgccggg tgcggtggga tccggagtgg caacggccgt
     7141 gatcgcaaga accggcgctc cgcctatagc ggtttcaacg cgattggaag ggacatccgg
     7201 gaatgcatac ccgtttgccc ccgtaaaacg ggcggttgcc gtgttcggga tgaccgtacc
     7261 cacgggcggc gaaagcgcca gcgcgaaagc agcgcaaaac gccagcagca tcgcggctcc
     7321 aagaaccgcg atggcccatc ggttaaccca tcttttcgac aacaaacctg tcatgacatc
     7381 cgccgattgc ccacgttccg aatctttgct ccggcgggga tttgctgcgc tgctttacaa
     7441 cgaaacaaat cctccgcctt aaaagcgatt atttaatggt cgccctgtac acgatcggaa
     7501 tcgtggcgcc tgcggcaatg ctcgggatcg aaatcataac cgtgccgctg actgcgtcat
     7561 aatgcccgaa atcgccgtcg gccgcgtcgg tttgccccgc gccggctacg gtgatgctgt
     7621 tcggcacgta attcaggtag ctcgacagca cgtcggtgac aacgaaggtt tgcgcgggcg
     7681 ccgcaccggt gttggtcacg gtgatcgtat aggtcaactg cgttcccgga ggctgcgggc
     7741 cggcgggtga aacgctcttg accagcccca tgacgggcgt ctgaatgttg gtgaacttga
     7801 tggatgtccc tgtgactgtt gtatcgtaaa cggaggtcgc ggtgatcgta tgcttgccct
     7861 gctgaccgtt ggaaccggcc acggtgacga cggcgacgta gaacttggtt tcgtcggggg
     7921 ccacggcgcc cgtgtcgatt ttgccgtcgg cgttgctgtc ggtgacgggc gtgtccacgc
     7981 cggccgtgta tttgccgtcc tgattcacgt cattatacac agcccaggtc cagtagagac
     8041 cgacggagct gatgctgtag atatccgtgc tgttgccggt attcttaatg gtgaagggat
     8101 gcaggttctg gtttcccacc agttcgtcgg ttgtcagcgt cgggggcatg acctgcacac
     8161 cggcggcctg ggacaccgta aaaggagacg tattggtatt gactgtggtc tccgtcccag
     8221 gatcgcctgc attggtccgg taggtggccg aggccgtgtt gttgatcacg gttgacgcag
     8281 cgatattggc gttgactgtg acctggaagc tgaaggtccc ggagcctccg gctgctacgg
     8341 tgccgatcgc ggcagtcact ttgcccggat tggtgacgtt gtagtccgcg ttgtccgcgt
     8401 cggccgcatc ggtgcgggcc gcaccgctgt aagtcatgga gccggttttg taggttgttt
     8461 cagccggaat ggcatccgtc agcacgacca ggtaacccgg cgccgtgccg ctgttgttcc
     8521 aggtaatgga gtacgtaacc gtttcaccgg gtttgggggc ggcgggcgtt gccgatttac
     8581 tgaaggtgat cacggccgcc tggatggtaa cggtcatcgt ggaggttgag cttacggccg
     8641 gcgtcaatac ggatgttgcg gtgaaattga gcgaggcggt gcttcccgcc gccgctccgg
     8701 ccggcgccgt aataaccgcg atggcatatt gaacctgatc cgcgtccagc gagacgcttg
     8761 cgcccagcat attgccggcg acctcttccg acggctgcag aatgccgtcg ccgttggtgt
     8821 ccttgtaaat ggcataggta tagccggccg gaagtccggc ggcggtcaga ttgaaggtgt
     8881 ccgtcccgtt gccggagttg acgatctgtc cggaaaagta ggttttgccg cccggagagc
     8941 cgttctggct gaccgtggcg ggtgacgtgg tcacgccggc cacctgactg accgttacgc
     9001 tcacggtgtt ggattcaacc ggcgcgtagg tgttgttgtt ggcgtcggtg taagttgccg
     9061 tggccttgtt ggtaatcgta gtgccggcgg gcgtgcccgc ggcaaatgcc gtgccggcgg
     9121 acagcatcgt ggacagcagc agtcctgcga tcagcaaaac gacgaactta agtggtgctt
     9181 ttactgtttt catgattgtt ctcctccctt gggttttatg tttttcatca gtttcttgtg
     9241 cttatttgac tttaacttta aaggtcaccg tcccggtcgc gttggccgga accggcgcct
     9301 tgatgatcca tttaatgtga gtgaaaagtt ccggcggagc gggttgaagg atttcctttc
     9361 cggctgcgtc caggcttttg cgcaaaacgg ggggctccac aaaagtcgcc ccgccgttga
     9421 tgctgaaaaa gatctttgcg tcctttccgg ctgcgcttcc cggcagatac accgtgtttg
     9481 ccggaacggg atcgacgacc gtgacgtctc ttgccacact cttgccggtg ttgtgataca
     9541 taacggtgta aacgagaata tctccgcttt taattttgtc cagcggcgcc gtggttacgg
     9601 tttttttgcc gttccggatt tgcgtcactt ccttttcgcc actgactttg agagcgatgc
     9661 gcggctcgtc ggcccgtgct tgcggtgccg ctagtaaaag cgccgccaag aacagcgctc
     9721 cccacaggac catccatcga aatgttttta cgttagtcat aatgcctcct tattgcttgc
     9781 gggtatttta aaaacttcct gttatcgtag agaaaagctg ttcatcaatc ttgtccctcc
     9841 ttcgcgaatc cctggcgtta tcggatcctc accttgaaat agacttttcc ttcatcgttg
     9901 cctgcgttgt ccgcaccgtc atcacccgcg actgtcgaga tgataaacgt gacattttcc
     9961 ccgttgatct gaccgacatc cgcatcggcg atatcggtca aactgtttgc cgtggcgtag
    10021 gtggaagcgg cgttcccgct tttaaggctg ccggttacat aagttgtgtt cgggggcacc
    10081 gtatcgaaaa tgatcagatt cgaggcggcg ctgcctccga cattccggta ataaatggta
    10141 aagaggacat cctgacccgg agtagccgtt gacggatcca cgtttttggt caaggtaatg
    10201 tgtggcatgg ttgtatccgt atcgtccgca gaattgttgc ccggcacggg atcggtcaca
    10261 ccggccggcg gggaaacggt tgccgtattg gtaaaagagc ccgcaaccgc cggatccacc
    10321 agaaccgtga gcgacagagc aatgctctgt ccgggcatca gattaagccc cgtccagagt
    10381 ccggttgctt cgttgtaaat tccctgtgac ggtttgaata ccgggctctg caaaagcggc
    10441 ggcagggtgt cgatgacggt gagactctcc acgcggcttg gaccgttatt ggtcacggtg
//
LOCUS       DAOSIM010000049        10391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_6274_length_10391_cov_7.579818, whole genome shotgun sequence.
ACCESSION   DAOSIM010000049 DAOSIM010000000
VERSION     DAOSIM010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 10391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10391
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6274_length_10391_cov_7.579818"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..>1020
                     /locus_tag="PLR47_03620"
     CDS             238..>1020
                     /locus_tag="PLR47_03620"
                     /inference="COORDINATES: protein motif:HMM:NF014027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HOG12058.1"
                     /translation="MSERISSNFPAVKEFIKDNYRHFNAAVVVDAAQAYVEHLQQGGK
                     MMIAMGGAMSTAEIGITLAQMIRADKVHAISCTGANLEEDIFNLVAHKHYRRVPHYRD
                     LTPADEADLLKRKLNRVTDTCIPEEEAMRKVEKKLLGFWKQAEEENKRYFPYEFIFQL
                     LSSGVLKSDYEIDEKDSWLIAACQKNIPIFVPGWEDSTLGNVFVSSVRKGQIQNPAVV
                     KSGLETLNALIDWYLDASRDRGVGFFQIAGGISGDFAISVVPL"
     assembly_gap    1021..1130
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1291..2196
                     /gene="zupT"
                     /locus_tag="PLR47_03625"
     CDS             1291..2196
                     /gene="zupT"
                     /locus_tag="PLR47_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOG12059.1"
                     /translation="MDSVFIALGLTLIAGMATGIGSVIAFTAKRTDYRLLSVATGFSA
                     GVMLYVSFVEIFPESTAMLAARYGESWGSRLVTASFFGGMILMALIDNVIPAAENPHE
                     THSEEETAPLHDPKAALPDFPSAAFRDSTHPKPGVHDHGPFRAGLLRTGLLTALAITI
                     HNFPEGIATFLAAMQEPALGAAIAVAVALHNIPEGISVSVPIFYATGSRKKAFWWSFA
                     SGLAEPLGAVAALAVITLITGGGAWNPPVNLTGFLFGGIAGVMVYISLDELLPTSRAY
                     SKGHDSILGLMAGMMVMAISLLLMR"
     gene            2327..5644
                     /locus_tag="PLR47_03630"
     CDS             2327..5644
                     /locus_tag="PLR47_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOG12060.1"
                     /translation="MMKRKSLKMIGAGFLWAAMFFATGAADCFAQQPKAPASPEKPEI
                     FVQLGHRSTDVKAAAFSSDGKYLVTGSGDKTVKLWHLSTGAEIRTFSGHEEEVTAVAI
                     SPDGSKILSGDKKGMIKLWDALSGNELRTMIVPWKEKEIWTLRFAPDGIRFSMLSGFS
                     LSFWDGGTGQALRKIDNIRGSYLADRGESYLAAKIGYKDDYALINPANAEEIKKFTYD
                     HQWFARASFSQDGKFVLFMTDDYSQKKYFFTLLDLTTGEKISSWQTAIEKDKNIYSIN
                     LLRDGKSALMSGGCGIELWDARKGKRLKVLTTSFTSMVNLSPDGRFFLSTGSRYAPTL
                     WDAASFSSVRSFRQRPLARCNSAQYRPDEKQVLINRTGAPPALLNLSTGGLEKTYSGY
                     SAGYFRAGGRSLLLIGEKGERLFQDLETHKVLKKFQSRYFDLPQDGKYLAELIGERDF
                     KVWETRADKEILNYREPSGSINSFTISSDNRHLVLCVDNRVKLIDIASGKETRSFTVP
                     DTANIHSAQISYDGRYLVAFYRDDVQQPKKLRAIVKIWNLETNQEIAKINNARNMLGF
                     KFSPDGRSLLVGDHTDRADSVWLIDLATGRIQKTLKGNDTIWATAFSADGRFLATGDA
                     DGSIMIWDVAAGRREMFVSGHKNGVRKIDFSSDNQQIVSTGDDGPARIWDIATGKEIA
                     QFFSFADGEWITITPEGYFNASAGGAKYLNVRLGNQIYSIDNFYETFFNPVYVASVLQ
                     GKKAEGLADIRTGIQSPPDVRILSPAPGAVLNTDTITIKVGAKDTGGGIDEIRLYHNG
                     KALGDDRRGVKLVGAGGETVRDFQVTLVDGTNIFRAVGFSSDRTESNPYEMTVSLSAP
                     SKDVSLYVFAVGVNQYRNPALNLNYAVPDAKGIAGFFRQKGQLFKKVNIIEIYDEKAT
                     KDGILAGLRELEKTNPQDAVLIYLAGHGENVKDVWYFIPHELAYPEREEYVQTKGISS
                     AELSSAMKNIRAQKILLLVDACKSGAVLVAFRGFEDRKALSQLSRSTGTHVVAASTKD
                     QFAAEVKDLGHGVFTYTLLEGLKGKAAGGSDTVTVRKLMGYIEEQLPEITKKYKQEAQ
                     FPVVDSRGMDFPLVIVK"
     gene            complement(5760..6188)
                     /locus_tag="PLR47_03635"
     CDS             complement(5760..6188)
                     /locus_tag="PLR47_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12061.1"
                     /translation="MKRKMFFAFLVMLVLLPVQLPAADFDWMPNFNIQAQADPSGFHA
                     RLATRFNIGDAQVNAVLSNFPKPADAYVALRLGEMSGKPIDDVTGQYKANKGKGWGAL
                     AKSLGIKPGSKEFHALKRGDDLYGPKGKGKSKAKKDKKNK"
     gene            complement(6221..6613)
                     /locus_tag="PLR47_03640"
     CDS             complement(6221..6613)
                     /locus_tag="PLR47_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12062.1"
                     /translation="MASKSREVRMEQRRILEKKLELRLQKLEALGAAKEKDPMVKNLR
                     SQIRETDIRIAAFDKNIAKIEILKQAKARKLAEKDAPREAPAAPEKEGKAKKKAAPAE
                     KESKKKAAPAEGEAPKKPKKKKEDAPAE"
     gene            complement(6734..7891)
                     /locus_tag="PLR47_03645"
     CDS             complement(6734..7891)
                     /locus_tag="PLR47_03645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOG12063.1"
                     /translation="MSIGERCRLNIQSVAFGGSGVGRAGGLVIFVPFTAAGDIADIEI
                     VERRKNFARGRLLGIVAPSPQRAEPLCRCFGRCGGCSYQHIAYHHQLNIKQAQVREAF
                     VKIGKLPDPPVEKVIASPRAYAYRGKAELHAAKSREGFRLGFMDITGGKLVDIERCEI
                     MHESINRQIIEARSRGRFESDGGDLIFWANPSSRAGDAIVRTVKDREFLIPLSGFFQT
                     NLYLTDAMVDEVRRLAGLEKRASILDACCGSGLFSIFLAQSAERLTGVEIHEKSVQCA
                     RENAERNGIRNAEFICADIGEHLNRMAERLDAVDLIVLDPPRAGLDSKALNAICGIKC
                     RDIIYVSCNPATQARDVKYLTANGYVLQSLQPLDMFSQTEHVETVGLLRRK"
     gene            8019..8858
                     /locus_tag="PLR47_03650"
     CDS             8019..8858
                     /locus_tag="PLR47_03650"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOG12064.1"
                     /translation="MSAQRNSILCPNCRKLISLDEPACPYCGLVRPGLHETAGKLRGI
                     LFAFEPVQTILYVNIAFFVLALLLDLRGIFTGGSPLAFLSPSQQSLLALGASGAIPVI
                     YYHNLWSIVSASFLHGGILHIVFNMMALYQLGPFVLREFGLHRFACIYILSGVAGFAV
                     SVLAGVQFTIGASASVCGLIGAIIYFGKSRGGSYGEAIFKQALGWVVGLIIFGFLFSG
                     INNWAHGGGLLTGLLLSFALGYSDQKPESAWSKILAYACVLITAAVLLWAAGSSLFYK
                     FLT"
     gene            8922..9317
                     /locus_tag="PLR47_03655"
     CDS             8922..9317
                     /locus_tag="PLR47_03655"
                     /inference="COORDINATES: protein motif:HMM:NF023514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HOG12065.1"
                     /translation="MKRNLCALIVLVFLLSGCDAAMVMGGKVMGVSSGRFIYQDGYLS
                     TQYKAGVDQVWQACEKTVVELKGGKIEKDRKIAGGSIKAVISDEKVHILVEYVEKNVT
                     NVSVMCGIGGSNIASRMLQDRIAANIVKP"
     gene            complement(9362..>10391)
                     /locus_tag="PLR47_03660"
     CDS             complement(9362..>10391)
                     /locus_tag="PLR47_03660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOG12066.1"
                     /translation="LGFFNSRTDISFRVVSRDTAAAVDASFWNRRVLDALELRRRIIG
                     EGTSCYRLINAEGDGFPGLIVDVYNNTLVLSVTTAGMELQKHAILDALLFHLKPERIY
                     EQSAGRSRGLEGLSERRGFLHGPGQESAARVTENGYRFDVDFICGQKTGFFLDQRVNR
                     ERIGSLARGRTVLNCFCYTGAFSVYAAGGGAKKVVSLDISKSACAAAAEHLRLNNCNP
                     EDHPVIEGDVFDYLRNLRECFDLIILDPPAFAKTKRDVTKASRGYKEINMQAIRHLSP
                     GGMLATFSCSNFIEEELFGKIVLGAARDAQADLQLLARLEAGPDHPLAAGHPEGRYLK
                     GLLLRKAA"
BASE COUNT         2240 a         2838 c         2795 g         2408 t
ORIGIN      
        1 attgttaatc tgttgatcaa ttctttaacg tttgggcttc atctgtttta catcgtcgtt
       61 catgagcaac ggcacaccgc aacattgata tatcgaaagg gagaacgctt tggtaaaaca
      121 gccccttcat attctgtttt ggaaatctgc tttcccgaag cgcggttctt ccctgaaacc
      181 gcggccggtc gtctgcggcg gacgcggctc tgccaaacgc atatcaggag cattcaaatg
      241 agcgaacgca tttcttccaa ttttccggcg gtcaaggaat tcatcaaaga caattatcgc
      301 catttcaacg cagccgtggt ggtcgatgcc gcccaggcgt atgtggaaca tcttcagcag
      361 ggcggcaaga tgatgatcgc catgggcggc gccatgagca ccgccgaaat cggcatcacc
      421 cttgcccaaa tgatccgggc ggacaaagtc cacgcgatct cctgcacggg agccaatctg
      481 gaagaggaca tcttcaacct ggtagcccat aagcattatc gccgtgttcc tcactaccgt
      541 gatctcacgc ccgccgatga agcggatttg ctcaaaagaa aattgaaccg cgtcaccgat
      601 acctgcattc ccgaagaaga agccatgaga aaggtcgaaa aaaagcttct ggggttttgg
      661 aagcaggcgg aagaggaaaa caaaagatat ttcccctatg aatttatttt tcagctcctt
      721 tccagcggcg tcctgaaaag cgactatgaa atcgacgaaa aggattcctg gctcattgcc
      781 gcctgccaaa aaaacattcc gatttttgtt cccggctggg aggattcgac gctgggcaat
      841 gtctttgtct cgagtgtccg gaaaggacaa attcaaaacc ccgccgtcgt caagtcaggc
      901 ctggaaacct tgaatgccct gatcgactgg tatctggacg cctcgcgcga ccggggcgtc
      961 ggcttttttc aaatcgcggg cggcatatcc ggagacttcg cgatcagcgt cgtgccgctg
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgctgttccc
     1141 aatgaaaaaa tcacctggga aaagctgtcg gtggatacgc cccgttttat catcgaaagc
     1201 gacgcgacta ttgttgctcc gctgattttc gcctacgttc tggcggaatg aaatgatttc
     1261 catcattgcg gaactgagag agactggaaa atggattctg ttttcattgc cctggggctg
     1321 acgctgatag ccggcatggc caccggcatc ggcagcgtta tcgcctttac agcgaagcgc
     1381 acagattacc gtctgctttc cgtggcgacg ggcttctcgg caggggtaat gctgtatgtc
     1441 tcctttgtcg aaatctttcc cgaaagcacg gcaatgctgg ccgcacgcta cggcgagtca
     1501 tggggaagcc ggcttgtgac cgcgtcgttt tttggcggca tgattttgat ggcgctcatc
     1561 gacaacgtga ttcccgccgc cgaaaacccc catgaaacgc attccgaaga agagacggcg
     1621 ccgcttcacg atccaaaggc ggcgcttccg gattttccgt ccgccgcatt cagggattct
     1681 actcatccta agccgggagt tcacgatcac ggcccgttcc gcgcggggct tttgcgcacg
     1741 ggactgctga cggcgctggc cattaccatt cacaattttc ccgaaggcat tgccaccttt
     1801 ctggccgcga tgcaggagcc ggcgctgggc gcagccattg ccgtggccgt cgccctgcac
     1861 aacattcccg aaggcatcag cgtttccgtc cccatatttt atgcaacagg cagccgcaaa
     1921 aaggcctttt ggtggtcttt cgccagcggt cttgccgaac cgctgggcgc cgtggccgct
     1981 ctggcggtga tcacgctgat caccgggggc ggcgcgtgga accctccggt gaacctgacg
     2041 gggtttctgt tcggcggcat tgccggggta atggtctata tcagcctcga tgaactcctg
     2101 cccaccagcc gcgcttacag caaggggcat gacagtattc tggggctgat ggccggcatg
     2161 atggtgatgg cgatcagtct gcttctgatg cgctagatcg gctcgtcatg aatacggcac
     2221 cttcgtcggc agcagccttg caaaatagcg cagagccgac taaaatagaa accggctttc
     2281 aataaagggc tgttttgaca gcaaccggca attcaggagt gagacgatga tgaaaagaaa
     2341 atcattgaaa atgatcggcg cgggattcct ttgggcagca atgttttttg ccaccggagc
     2401 ggccgactgt tttgcccagc agccgaaagc accggcaagt cccgaaaaac cggaaatttt
     2461 cgtgcagttg ggacatcgca gcaccgatgt gaaagccgcc gctttttctt ccgatggaaa
     2521 atatctcgtg acgggaagcg gcgacaagac cgttaagctc tggcatcttt ccacgggcgc
     2581 cgaaatccgg acgttttccg gccacgagga agaagtgacg gccgtggcga tttcgccgga
     2641 cggctcgaaa atactctccg gcgataaaaa gggaatgatc aaactttggg acgccttgtc
     2701 cggcaacgag ttgcgcacca tgattgttcc ctggaaagaa aaagaaatct ggacgctgcg
     2761 ctttgcgcct gacggaattc gttttagtat gctgtcggga ttttcgttgt ccttctggga
     2821 cggcggaaca gggcaggcgc tgagaaaaat cgataatatc cgcggcagtt atcttgccga
     2881 ccgcggcgaa tcgtatctgg cggcaaagat cggctacaaa gatgattatg cgttgatcaa
     2941 tccggccaat gcggaggaga ttaagaaatt tacttatgat catcaatggt ttgcaagggc
     3001 atccttttcg caggacggta aatttgttct ttttatgaca gatgactaca gtcagaaaaa
     3061 atattttttc acgctgctgg atttgacaac gggtgaaaaa atatcttcct ggcagacggc
     3121 gattgaaaaa gacaaaaaca tctattcaat caatcttctc cgtgacggaa aatcggcctt
     3181 aatgtctggt gggtgcggca tagagctttg ggatgcccgg aagggtaaaa ggctgaaggt
     3241 attgacaacc agttttacct ccatggttaa tctttctccg gacggaagat tttttctttc
     3301 gaccggttcg cgttatgcgc cgacgctttg ggatgcggca tcattttcta gtgtgcgatc
     3361 cttccgccag cggccgctgg cgcggtgcaa tagcgcgcag taccgtccgg atgaaaaaca
     3421 ggtgctgatc aatcgaaccg gcgcgccgcc ggcccttttg aatttgtcaa cgggcggcct
     3481 ggagaaaacc tattccggat attcggcggg atatttccgc gccgggggcc gctctctttt
     3541 gctgatcggc gaaaaaggcg agcgtctctt ccaggatctg gagacccata aggttttgaa
     3601 aaaatttcaa tcccggtact tcgatctgcc gcaagacgga aaatatctgg cggaactcat
     3661 cggcgagcga gacttcaaag tatgggagac cagagcggat aaagaaattt taaactatcg
     3721 ggagccttcc ggatccatca atagcttcac gatttccagc gataaccggc atcttgtgct
     3781 gtgcgtggac aaccgcgtca aactgatcga tattgcctcc gggaaagaaa cgcgctcttt
     3841 tactgttccc gacacggcaa atattcactc ggctcaaata tcgtatgacg gccgttatct
     3901 tgtcgctttt tatcgtgacg atgttcagca accgaaaaag cttcgggcga ttgtgaagat
     3961 atggaatctg gagacaaacc aggagatcgc gaaaatcaat aacgccagaa atatgctggg
     4021 ttttaaattt tcgcctgacg gcagatcgct gcttgtcggt gaccataccg atcgggccga
     4081 cagcgtctgg cttattgatc tggcgaccgg tcgaattcag aaaacattga aaggaaacga
     4141 cacaatctgg gcaacagcct tttccgcgga tggaagattc ctggcaaccg gcgatgccga
     4201 cggaagcatc atgatctggg atgtggcggc gggcaggcgg gaaatgtttg tctccggaca
     4261 taaaaacggc gtccggaaaa ttgatttttc atctgacaat cagcagattg tgtcgaccgg
     4321 tgacgacggc ccggcccgta tatgggatat cgcaaccggc aaggaaattg cgcagttctt
     4381 ttcctttgcc gacggcgaat ggattaccat tacaccggaa ggatatttca acgcttcggc
     4441 cggcggtgcc aagtatctga atgtgcgtct ggggaaccag atttattcga tcgataattt
     4501 ttatgaaaca ttttttaacc cggtttacgt tgcttctgtc ctgcagggga aaaaggcgga
     4561 agggcttgcc gatattcgca cgggcattca atcgccgccg gatgtccgga tcctctcgcc
     4621 cgcgcccggc gccgtcctga acaccgacac catcaccatt aaagtcggag ccaaagacac
     4681 gggcggcggc atcgatgaaa tccgcctcta ccacaacggc aaggcgctgg gcgacgatcg
     4741 tcggggagtg aagctcgtgg gcgccggcgg cgagaccgtc agggattttc aggtgacgct
     4801 ggtggacgga accaacattt tccgcgccgt gggcttcagc tccgaccgca ccgaatccaa
     4861 tccctatgaa atgaccgttt cgctcagtgc gccttccaag gatgtgtcgc tttatgtttt
     4921 tgccgtcggc gtcaaccaat accgcaatcc ggcgctgaac ctcaactatg ccgtgccgga
     4981 cgccaaaggc attgccggct tcttccggca gaagggtcag ctattcaaga aagtgaacat
     5041 catcgagatt tacgatgaaa aagcgacgaa agacggcatt cttgccgggc tgcgcgaact
     5101 ggagaaaacc aatccgcagg acgccgtgct gatctatctg gccggccacg gcgaaaacgt
     5161 gaaggatgtc tggtacttca tcccccatga gctggcctat ccggagcgcg aagaatatgt
     5221 tcagaccaaa ggaatatcgt ccgcggaatt gtcctcggcc atgaaaaata ttcgggcgca
     5281 gaaaatcctg ctgctggtcg atgcctgcaa atccggcgcc gtactggtcg cctttcgggg
     5341 ctttgaagac cgcaaggcgc tctcacagct ttcaagatct acgggaacgc atgtcgtcgc
     5401 ggcatccacc aaagaccagt tcgccgccga ggtgaaggac ctggggcacg gcgtgttcac
     5461 ctatactttg ctggaaggtt tgaagggaaa ggccgcgggc ggcagcgaca cggtcacggt
     5521 gcgcaaactg atgggctata ttgaagagca gcttccggaa ataaccaaaa agtacaaaca
     5581 ggaagcgcag ttcccggtgg tggattcccg cggaatggat ttcccgctgg tgatcgtgaa
     5641 atagaagcac gccgaagcac tgaaaatgaa tttggtttac ccgccttatc cgaaagcggg
     5701 caacctttat tcagtgcaga cgacacctat cgtgcccttc tcttctgccg tccggaatcc
     5761 tatttatttt ttttgtcttt cttagccttg cttttcccct tgcccttcgg accgtaaaga
     5821 tcatctcctc gtttgagggc atgaaattcc tttgagccgg gtttgatgcc caggcttttg
     5881 gccagcgctc cccatccctt ccccttgttt gccttgtact gcccggtgac gtcgtcgatc
     5941 ggctttcccg acatttcccc caggcgcagc gcaacgtagg catccgccgg tttgggaaaa
     6001 ttgctgagaa cggcgttcac ctgcgcatcg ccgatattaa agcgcgtggc aagccgggcg
     6061 tggaatccgg acggatccgc ctgtgcctga atgttgaaat tcggcatcca gtcaaaatcc
     6121 gccgccggaa gctgaacggg aagcagcacc aacatcacca aaaacgcaaa aaacattttt
     6181 cttttcatgt tgccctccca tagattttgt tttggcggca ttattccgcc ggagcgtctt
     6241 ctttcttttt ctttggtttc ttcggggctt ctccttcggc cggagccgcc ttctttttgg
     6301 attccttctc cgccggggcg gctttcttct ttgccttccc ttctttttcc ggcgcggccg
     6361 gggcttccct gggtgcatcc ttttccgcca gtttcctggc cttggcctgt ttcaatattt
     6421 cgatttttgc aatgttttta tcaaaagcgg caatgcggat gtccgtttca cgaatttgcg
     6481 atctcagatt ttttaccata gggtcttttt ctttggcggc cccgagcgct tccaatttct
     6541 gaagccgaag ctccagtttc ttttccagta tccggcgctg ttccattctg acttccctgc
     6601 tttttgatgc catcttatcc tcccttgaaa atagataaat aatctcgatt tgccggggaa
     6661 agtagcaata ttttcaccag aaagccacca ttttttaaag aaccgtcccg gctttcgtct
     6721 cttcccgtcc cggttatttc ctcctgagca ggccgacggt ttcaacatgc tcggtttgcg
     6781 aaaacatatc aaggggttga agactttgca gaacgtatcc gttggcggtc aggtatttga
     6841 cgtcccgagc ctgcgtggcc ggattgcagg aaacataaat gatatcccgg cattttatcc
     6901 cgcatatggc gttcagcgcc tttgaatcca ggccggcgcg cgggggatcc agcacaatga
     6961 gatcaaccgc atccagcctt tcggccatgc ggtttaagtg ctccccgatg tcggcgcaga
     7021 tgaactccgc gtttcttatt ccgtttcttt cggcattttc ccgcgcgcac tgaacggact
     7081 tttcatgaat ttcaacgcct gtcagccgtt cggcgctttg cgccagaaag atggaaaaaa
     7141 gccccgaccc gcagcaggca tccagaatgg acgccctttt ttccaggccg gcaaggcggc
     7201 gcacttcgtc caccatggca tcggtcagat acaaattggt ctgaaagaat cccgagagcg
     7261 ggatcagaaa ttcccggtcc ttcacggttc tcacaatagc atcgcccgcg cggcttgacg
     7321 gattggccca gaaaatcaga tcgccgccat ccgattcaaa gcgtccccgg gatcttgcct
     7381 cgatgatctg ccgattgatg gactcatgca tgatctcgca gcgttcaatg tccaccagct
     7441 ttccgcctgt gatgtccatg aatcccaggc gaaagccttc ccgtgatttg gcagcgtgca
     7501 gttcggcctt tcccctgtaa gcataggcgc gcggcgacgc gatgactttt tcaaccggcg
     7561 ggtccggcaa tttgccgatt tttacaaaag cctcccgcac ctgggcctgc ttgatgttca
     7621 actgatgatg gtaagcgatg tgctgatagg agcagccgcc gcaccggccg aagcaacggc
     7681 agagcggctc cgctctctgc ggcgagggcg ctacgatgcc cagcagacgc ccccgggcga
     7741 aatttttcct gcgctcaacg atttcaatgt ccgcaatgtc ccccgctgct gtaaacggca
     7801 caaaaatcac cagaccgccg gcccgcccga cgccggatcc gccaaaggcg acagattgaa
     7861 tgttcaaacg gcagcgttct ccgatgctca tgatcctcct gacgcccggc actatcctat
     7921 attgcggggc gggagtcaac cgcgcggacg catttttctt tacaactctt ttttttgttg
     7981 ttaaggattc cctccacaaa aacgagaagg aactcacgat gtccgctcaa agaaactcca
     8041 tactctgccc gaactgccgc aagctgatca gtctggatga accggcctgc ccctactgcg
     8101 gactggtgcg tcccggcctg cacgaaacgg ccggaaaact gcggggcatc cttttcgcgt
     8161 ttgagccggt gcagacgatc ctgtacgtaa acatcgcttt ctttgtcctt gcacttctgc
     8221 tggatctgcg gggcattttt acgggcggca gtccgctggc gtttctttcg ccttcccagc
     8281 aaagcctgct ggcgctcggg gccagcggcg ccattcccgt catttattac cacaacctct
     8341 ggagcattgt ttccgcctcg tttctgcacg ggggcattct gcacatcgtt ttcaacatga
     8401 tggcactcta ccagctcggc cccttcgtgc tgcgtgaatt cgggctccac cgtttcgcct
     8461 gcatttacat tctgtccggc gtcgccggtt ttgccgtttc ggtgctggca ggcgtgcagt
     8521 tcaccatcgg cgcgtccgct tccgtctgcg ggctgatcgg cgcgatcatt tatttcggga
     8581 aaagccgcgg aggctcttac ggagaggcca ttttcaaaca ggcgctgggc tgggttgtgg
     8641 ggctgatcat cttcggattt ctcttcagcg gcatcaacaa ctgggcgcac ggcggcggcc
     8701 ttcttacggg cctgcttttg tccttcgcgc tgggctacag cgaccagaag ccggaaagcg
     8761 cctggagcaa aattcttgca tacgcctgcg tgctgatcac cgcggccgtt ctgctctggg
     8821 cggcggggtc gtcgttgttt tacaaattct taacgtaagc tgaaagcgcc atgtcctctg
     8881 cttcatccgg cggacacggc cgatcgccag gagggaaatt catgaaaaga aatctttgcg
     8941 cgctgatcgt tttggttttt ctgctgtccg gttgcgacgc cgccatggtc atgggtggaa
     9001 aagtcatggg cgtcagctcc ggaagattca tctatcagga cggctacctc tccacgcagt
     9061 acaaggccgg cgtggaccag gtatggcagg cttgtgaaaa aaccgtagtg gagctgaaag
     9121 gcggcaagat cgaaaaggac cgcaaaattg ccggcgggtc gatcaaggcc gtcatctcgg
     9181 atgaaaaggt gcacattctg gtcgaatacg tcgaaaagaa tgtgaccaat gtgtccgtga
     9241 tgtgcggcat cggcggcagc aacattgctt cgcggatgct ccaggacagg atcgccgcca
     9301 atatcgtaaa gccctgacga agcgtgaagg catgcaggca aagcggcgcc gtttgcgccc
     9361 gtcatgccgc ttttctcagc agcagtcctt tcagataccg gccttcggga tggcccgcgg
     9421 ccagcggatg atccggcccc gcttccaggc gggccagcag ttgcaggtcg gcctgcgcgt
     9481 cgcgcgcagc gcccagaaca atcttgccga ataattcttc ttcgatgaaa ttggagcagg
     9541 aaaaagttgc cagcattcca cccggcgaca gatgccggat ggcctgcata ttgatttcct
     9601 tgtagccgcg ggaggctttg gtgacatctc ttttggtctt ggcaaacgcg ggcggatcga
     9661 gaatgatcag atcgaaacat tcccgcagat tgcgcagata gtcaaagaca tccccctcga
     9721 tgaccgggtg gtcttccggg ttgcagttgt tgaggcgcag atgctccgcg gccgccgcgc
     9781 aggcggactt ggaaatgtcc agagaaacta cttttttggc cccgcccccg gcggcgtaaa
     9841 cggaaaacgc gccggtgtag caaaagcagt tcagcaccgt cctgccccgg gccagggaac
     9901 cgattctctc ccggttgacg cgctggtcga ggaaaaagcc ggttttctgg ccgcaaataa
     9961 aatccacgtc aaaccggtag ccgttttccg tcacgcgcgc ggcgctttcc tgaccaggtc
    10021 cgtggagaaa ccccctgcgc tccgacaggc cttccagtcc gcgcgagcgc ccggcgcttt
    10081 gttcgtagat gcgttccggt ttcaggtgga aaagcagggc gtccagaatc gcatgcttct
    10141 gcagctccat cccggccgtc gtgacggaca aaaccagcgt gttgttgtaa acatccacaa
    10201 tcaggccggg aaagccgtcg ccttcggcat tgatcaggcg atagcagctc gtcccctcgc
    10261 cgatgattcg cctgcgcaat tccagtgcgt ccagaactct ccggttccag aaggatgcat
    10321 cgacggcggc ggccgtgtcg cggctgacca cacggaagga aatatccgtg cggctgttga
    10381 aaaatcccag g
//
LOCUS       DAOSIM010000050         9839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_6661_length_9839_cov_6.074918, whole genome shotgun sequence.
ACCESSION   DAOSIM010000050 DAOSIM010000000
VERSION     DAOSIM010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9839
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6661_length_9839_cov_6.074918"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLR47_03665"
     CDS             <1..228
                     /locus_tag="PLR47_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12067.1"
                     /translation="ALHDNNHTSPLKKKAMAFAAMALKTEKPWAALIRSTHGCFGFVT
                     LPSPSQRQADRRTVIKPVAKRIAVCVHLSVH"
     gene            complement(291..932)
                     /gene="thiE"
                     /locus_tag="PLR47_03670"
     CDS             complement(291..932)
                     /gene="thiE"
                     /locus_tag="PLR47_03670"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOG12068.1"
                     /translation="MRGIYLVTDRGLCRGRSLLEVVRMAVQGGASCVQLREKDLPTRA
                     FVEEALAVKNILAPHRVPLMINDRIDVALACGADGIHVGQEDMPYETARRLMGPKAII
                     GLSVETWADVEASRDLDVDYLGVSPVFATPTKTDTKEPWGLEGLRKIKAFSRHPLVAI
                     GGISQINAREVAEAGADCLAVVSAICSAGNPAEATARLKHAFDVALRLRDNHK"
     gene            complement(935..1738)
                     /gene="thiM"
                     /locus_tag="PLR47_03675"
     CDS             complement(935..1738)
                     /gene="thiM"
                     /locus_tag="PLR47_03675"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOG12069.1"
                     /translation="MKLSASDIYQSVEEIRAKSPLIHNITNYVVMNNTANALLAIGAS
                     PVMIHAEEEVEDMAAIASALVINIGTISPSWVRGMFKASAKAVSKGVPVVIDPVGAGA
                     TPYRTGTVRDLIRAGRPTIIRGNASEILALTDDRLKTKGVDSTAASDEALHAAQSIAD
                     ASQCAVCVSGAVDYIVEGKRLVKVANGHPMMPKVTGLGCTASALCGAFAAVDQDPLTA
                     AAKAMAVMGIAGEIAAEQSSGPGSLQMHFIDALYRLAKEDIERRLKVEG"
     regulatory      complement(1800..1901)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2044..3180
                     /locus_tag="PLR47_03680"
     CDS             2044..3180
                     /locus_tag="PLR47_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12070.1"
                     /translation="MAARRQILVFFQIIVLLMMGGIISACHHKNTDSAGISVDQFTAE
                     LKMDGFEVAQGYFQLWRIEDCPESFDVMGTCYFNNPTAPYVMSVLPFWPDEYIDPATK
                     NAFGSTKLGCSTSFRFDPNEAIVIFGFLPPKAVYFGLQSYLFTRKDAYKTDNDTYNFI
                     NRIGAKDVFFHTVPGRPAQDRILTFDSLSDSKNNVVIERQSDGSFGQFRYFIITPDRY
                     MDQKIRRILHNLSVADQEIFTEGIPANMRIGLGEDADDFVSGIRYSLPEDGGKEGTPS
                     DIWRNNPPLSILRIRTSQPHPPQPYPAWEDNSPEPRKAVSEADLKDDLNLLVQKVSQA
                     WGQPCIDTAGCSDKARSFIDTQSSPFNGLSRGHAGRNLPIQRRL"
     gene            3356..3586
                     /locus_tag="PLR47_03685"
     CDS             3356..3586
                     /locus_tag="PLR47_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12071.1"
                     /translation="MDKLYIYYFTRNCEDEKVQELTHGFCLSVKDTELVVPPGDKVSL
                     VERDYMAPGTQRGPNSKLTLPSMVLILQKPGQ"
     gene            4037..4312
                     /locus_tag="PLR47_03690"
     CDS             4037..4312
                     /locus_tag="PLR47_03690"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOG12072.1"
                     /translation="MQTNIKYSEDVVPITDLKINPGRIVNQVDKTRRPVLLTSRGRGV
                     AVVQSVKDYEERTEEQGFLRAVVRGLMDIEEGREISLNEVKKRLGLK"
     gene            4315..4617
                     /locus_tag="PLR47_03695"
     CDS             4315..4617
                     /locus_tag="PLR47_03695"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOG12073.1"
                     /translation="MPEKKRTITFAASAFDDLEGIRAWYQERQVPDVGNKLLGEIIFQ
                     MERLADFPESGRIVPEFGIANLREIIHPPFRIVYRLDGHKIRIIRVWRSERLLKIP"
     gene            complement(4716..7775)
                     /locus_tag="PLR47_03700"
     CDS             complement(4716..7775)
                     /locus_tag="PLR47_03700"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HOG12074.1"
                     /translation="MPAPQIILDLIERFERNKEAYKSGAYNETQVRREFIDPFFQALG
                     WDVNNEAGNAEAYKDVIHEDAVKVGGVTKAPDYSFRIGGQRKFFVEAKQPSINIKDTP
                     APAYQVRRYAWSAKLPLSIVTDFDEFAVYDCRVKPDQSDRADKARILYYTYKQYDEKW
                     DEIAGIFSKDAILKGSFDKYAEFNKGKRGTAEVDSAFLAEIESWREMLARNIALRNTL
                     SVRELNDAVQRTIDRIIFLRIAEDRGIEEYGSLRGLCNGNNAYARLRELFYKADDRYN
                     SGLFHFRHEKNEAEPPDNLTTSLTIDDKPLKDILKRLYYPDSPYEFSVLPADILGQVY
                     EQFLGKVIRLTSDHRAVVEDKPEVKKAGGVFYTPTYIVDYIVQNTVGKLLEGKTPKTA
                     EKLRILDPACGSGSFLLGAYQHLLDWHVKWYAENDPEKCARAKKPVLFKGQGGAWHLT
                     TAERKRILLNNIYGVDIDFQACEVTKLSLLLKVLEGETGETLNYQFKLFHERALPNLG
                     GNIKCGNSLIGPDFYDGKQTSLLDTEEMQRVNAFDWQAEFPDIFKAGGFDAVIGNPPY
                     IRIQALKEWAPKEVEFYKEAYQSAQKGNYDIYVVFVEKGLQLLDKTGRLGFILPHKFF
                     NAQYGEPLRKLISDGKHLSEIIHFGDKQIFVNATTYTCLFFLEKQIQRNFNFEKVMDV
                     EMWRSNIGLKKKRNILPSDLISAAEWNFVTGPSRALFERMRTLPNKLDHIADIFVGLQ
                     TSADKVFIMNLIRDNGSTLTLKSLALNQEWELEKELLHPLVSGTDVNRFCPLSNRQYI
                     IFPYNVSDGVKLIDFKFIQMNYPRTAEYLLANKSILEKRENGKVKNAGWYGYIYLKNM
                     SRQSLTKICVPRLVEHLYAAIDHDGSHYLDNVDVGGITLKSDYEDQGLHYLLGLLNSK
                     VLRWFFPFVSAPFRGGWLSANRQFLSQVPFRIIDFKNEADKVSHAKITQLVEQTLTLH
                     KQLHEAKTGHEQTLLQRRIDATDKQIDKLVYELYGLTEDEIKIVEGR"
     gene            7939..8400
                     /locus_tag="PLR47_03705"
     CDS             7939..8400
                     /locus_tag="PLR47_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOG12075.1"
                     /translation="MKKAPLVFGIGQCSLDHLGLVPVYPPPDVKCEFTNLVLQGGGPA
                     ATALVALKRWGARCHIAGVLGDDGFGTQIQALLSAEDIDTTGLQIRKQHQSQFAFIAS
                     EPALARRTIFYQMPTGAPLRPEEINTGILLKSQALHTDGLFTEASLFAWRK"
     gene            8397..8774
                     /locus_tag="PLR47_03710"
     CDS             8397..8774
                     /locus_tag="PLR47_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOG12076.1"
                     /translation="MSDCFVTSEVFSNAFAETPAETCRKLAAMGVRLPGVTLGKRGYV
                     ALVDGRFIEKEAYPAKAIDTTGCGDVFHAGLIHGILHGWSAEKCLDLGAWAAARVSEQ
                     MGGRSGIPDIRTLQSKYPGDHGR"
     gene            8973..>9839
                     /locus_tag="PLR47_03715"
     CDS             8973..>9839
                     /locus_tag="PLR47_03715"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG12077.1"
                     /translation="MSDYEVVVIGAGNGGLTAAAGLAKRGHKTLLLERHNVPGGCATS
                     FVRGRFEFEVALHQLSGMGLPEFPGPLRGLLNDLGVMDKIEFVQQKNLYRVVVPGQLD
                     INLKADRAAVIESLTERFPAEKEGIQKFFDLLYAFCNELVNVLFIHDPEASPEKYPLF
                     FNLALKNSQGILDDYLKDRLLQAAVTIYWSYAGQPPSRLSFMELAMMIWAYIEFKPWH
                     MKGGSQAMSNAICDAFLRCGGDARFNCAARKILVKNGRVAAVVTEDGEEIATKFVVSN
                     AGPIRTLIDMVGA"
BASE COUNT         2324 a         2583 c         2535 g         2397 t
ORIGIN      
        1 gctcttcatg acaataatca tacatccccc ttgaaaaaaa aggccatggc ttttgccgcc
       61 atggccttaa aaacagaaaa gccatgggcg gctctcattc gatccaccca tggctgtttt
      121 ggttttgtta ccttaccaag tccttcccag cgacaggcag accgacgcac cgtaataaaa
      181 ccagtagcca aaagaattgc tgtttgcgtt catctgtctg ttcattgaaa aaaccgtcct
      241 tttctttcgt agccctataa acgagcgctc cgggcttgtc aagaaaattt ttatttatga
      301 ttatccctca ggcgcagcgc cacatcaaag gcatgtttca gtctcgcggt tgcctcagcc
      361 ggattcccgg ccgagcagat ggccgaaaca acagccaggc aatcggcgcc ggcttcggcc
      421 acttcccggg cattgatctg gctgattccc ccgatggcca ccagggggtg acggctgaac
      481 gccttgattt ttctgagccc ttcaagtccc catggttcct ttgtatcggt tttggtcggc
      541 gtggcaaaca cggggctgac ccccaggtaa tcgacatcca gatcgcggct ggcttcgacg
      601 tccgcccagg tttccacgga aaggccgatg atcgctttcg gccccatcag gcggcgggct
      661 gtttcgtacg gcatgtcctc ctgaccgaca tgaatgccgt ccgcgccgca ggccagcgct
      721 acatcgatcc ggtcattgat catcagggga accctgtgag gcgcaaggat gttctttacg
      781 gcaagggctt cctccacgaa agcgcgcgtg ggcaagtcct tttcccgcag ctggacgcag
      841 gacgcgccgc cctgaacagc cattcgaact acctccagca gagatctgcc gcggcataat
      901 ccgcggtccg tcaccagata gatgccgcgc atggctagcc ctccactttc aggcggcgct
      961 caatgtcctc tttggccaaa cggtaaagcg catcgataaa atgcatctgg agtgagccgg
     1021 gtccggagga ttgttcggcg gcgatctctc cggcaatgcc catgaccgcc atggctttgg
     1081 ccgccgctgt cagcggatcc tgatccaccg ccgcaaaagc gccgcaaagc gcgctggctg
     1141 tacagcccag ccccgtgact ttcggcatca tcggatggcc gttggctact tttaccagac
     1201 gcttcccttc tacgatatag tccacggcgc cgctgacgca aaccgcgcac tgagaggcat
     1261 cggcaatgct ttgcgcggcg tggagtgcct cgtcggatgc ggcggtgctg tccaccccct
     1321 tcgttttcag acggtcatcc gtcagcgcaa gaatttccga tgcattgccc cggataatgg
     1381 tcggccggcc ggcgcggatc agatcacgaa cggtgccggt tctgtaaggc gtcgcccctg
     1441 cgccgaccgg atcgatcacc acgggaacac ctttggaaac cgctttcgca gacgccttga
     1501 acatgccgcg cacccaggag gggctgatcg tcccgatgtt gatcaccagc gcggaggcga
     1561 tcgccgccat gtcttccact tcctcctccg catggatcat gaccggcgac gcgccgatcg
     1621 ccaaaagcgc attggccgtg ttgttcatga ccacgtaatt ggtgatgttg tggatcaacg
     1681 ggcttttggc ccggatttct tcaacggatt gataaatgtc cgatgccgac aatttcatac
     1741 ctcatctcct tgtttccatt tcattaaaaa gccccttctc caccgcggga gaaagggcct
     1801 aaagggttgc tcttttctcc ctccgccggc attatccgga tcaggttcaa cgggtataat
     1861 ctcagacaag gccaaagacc ctgacacccc aatgtgtttc gcttaccagc ctctcccgta
     1921 tttgtcaata catcacccat gtcccaaaat ccccttgaca tacactagtt cgtttaatat
     1981 agttttcctc aaaatagaag ttcttatcaa ttctccatat gaaaattttt aggaggcact
     2041 ctgatggctg cacgtcgaca gatcctcgtt ttttttcaga tcatcgtctt actgatgatg
     2101 gggggaataa tttcggcatg tcatcacaaa aacaccgatt ccgccggcat cagcgtcgat
     2161 caattcacgg ctgaattgaa gatggacggc tttgaagttg cccaaggcta tttccagctg
     2221 tggaggatcg aggactgccc ggaatccttt gacgtgatgg gaacctgcta tttcaataat
     2281 cccaccgcgc cttatgtgat gtcggtttta cctttctggc cggacgagta cattgacccg
     2341 gcaaccaaaa acgcttttgg ctcgaccaag ttgggatgca gtacaagctt ccgttttgac
     2401 ccgaatgaag caattgtcat tttcggattc ctgcctccga aggccgtcta tttcgggttg
     2461 cagagctacc tgttcacccg caaagacgcc tataaaacag acaacgatac gtacaatttt
     2521 atcaaccgga tcggcgccaa agacgttttc ttccacacgg tccctggccg tcccgcgcag
     2581 gaccgcattt taactttcga cagcctgagc gacagcaaaa acaacgtggt cattgaaagg
     2641 cagtcagacg gaagcttcgg tcagttccgg tacttcatca tcacgcccga ccggtatatg
     2701 gatcagaaga tccgacgaat cctgcacaat ctttcggttg ctgatcaaga aatctttacc
     2761 gagggcatac ccgcgaacat gaggattggt cttggtgaag acgccgacga tttcgtttcg
     2821 ggaattcgtt actctctccc ggaagacggc ggcaaggagg gcactccctc ggatatctgg
     2881 aggaacaatc caccgctgtc aattctgcgg atccgcacct cacaaccaca cccgcctcag
     2941 ccttatccgg catgggagga caacagtccc gaaccgagaa aggcggtatc cgaagcggac
     3001 ctcaaggacg atctcaactt gctcgtgcag aaagtcagcc aggcatgggg gcagccatgc
     3061 attgatactg ccggttgctc agacaaggcc aggtctttta tcgacacgca gtcgtccccg
     3121 ttcaatggac tgtctcgcgg acacgcagga cgcaacttac caattcagcg gcggctatga
     3181 gtttgacaac ggcgaagtct atgcggtgat tggaaccctg ggtacggcga ccggaaacgc
     3241 aagctacgtc agcctggggg tcaacaactt ccgcctaagg ctgggcgcag agaatgtcga
     3301 cggctcgaag ctcgagggca gcgtgaagcc ggagtggtac ccgggagtca ataacttgga
     3361 taagctctac atctactatt ttacccgcaa ctgtgaagac gagaaagtgc aagagctgac
     3421 ccacggcttc tgcttgtccg tgaaagatac ggagctcgtg gttccgcccg gtgacaaggt
     3481 ttcgctcgtc gagcgcgatt acatggcccc ggggacacag cgggggccta actcgaagct
     3541 tactctacct tccatggtgc tcatacttca gaagcccgga caataaaaaa actcttatgg
     3601 ataacagcta aacaggaaca acagcgtcca tccatttcca ggctgttgcc ttcaatacaa
     3661 ttgaggatct ctttttcagg aaatgattcc tacggtcaag atgcgccttc cacgatggcg
     3721 atttcaatga acctcgccaa gaacgagctt tgcgaagttc gaagcgtaag ttgaattgtc
     3781 ccaggagcga agcgacgcgg ggcttccggt gtgaaatcgt agagttcgta agccagttga
     3841 tctttccgtt gaaccatcct atcgatccgg tgatggttca gttatggcat ataattgatt
     3901 tttcggcccg cagcattctt ataaatgaca atattcatgg aatgagcccc cgaataatct
     3961 tttcatgaaa gtttttgaca ggtcgtttaa ccggtgctat tatcagcatc gttgaaagat
     4021 cgtagaggag ggttttatgc agaccaatat caaatattcg gaagacgtcg ttccgatcac
     4081 cgatctgaag atcaatccgg gacggattgt caatcaggtg gataaaaccc gtcggcctgt
     4141 gctcttgacc agccgtggac ggggtgtcgc cgtggtgcaa tcggtaaaag attatgagga
     4201 aaggacggag gaacagggat tcctgcgcgc cgtcgttcgc ggcctgatgg atattgaaga
     4261 gggtcgggaa atcagcctca atgaagttaa aaaacggctg ggactaaagt agacatgccc
     4321 gaaaaaaaga gaaccattac ttttgcggcg tcggcctttg atgatcttga aggcatccgc
     4381 gcatggtatc aagagcggca agtgccggac gtcggcaaca aactgctggg tgaaattatt
     4441 tttcaaatgg aaaggcttgc cgattttccg gaaagcggac gcattgtgcc ggaattcggc
     4501 attgccaatt taagagaaat tatccatcca ccctttcgga ttgtttaccg ccttgatggt
     4561 cataaaatca gaattatccg cgtctggcgc agtgaaagac tcttaaaaat tccgtaaaag
     4621 aaattgcggt aaagccgtta ttttgcggat tgatcgggga tcaatcccta caacaaattt
     4681 cctgttgggc gggttccccg aacactccgc atatattatc gcccctcgac aattttaatt
     4741 tcgtcttccg tcaggccgta gagttcataa accagtttgt cgatttgctt gtcggtggcg
     4801 tcgatccggc gctggagcag cgtttgctcg tggccggttt tggcttcatg caattgcttg
     4861 tgcaaggtga gcgtttgttc gacaagttga gtaattttag cgtgacttac cttatcggct
     4921 tcgttcttga aatcgataat acgaaaaggt acttgagata aaaactgtcg gttcgcagat
     4981 agccaaccac ccctaaaagg agcgcttaca aaagggaaaa accaacgcaa tacttttgaa
     5041 ttgagcagtc caagcagata gtgcaggccc tgatcctcgt aatcagattt caaagtaata
     5101 ccaccaacat ctacattatc taaataatga ctgccatcat ggtcaatagc agcataaagg
     5161 tgttcgacta agcgtggcac acagatcttt gtaagagatt gcctactcat atttttaaga
     5221 taaatatagc cataccaacc agcgttttta acttttccat tttcacgctt ttccaatatg
     5281 cttttatttg ccaataaata ttccgctgtt ctcggataat tcatctgaat aaacttaaaa
     5341 tcgataagct tcactccatc agatacatta tatggaaaaa ttatgtattg ccggtttgac
     5401 agaggacaga atctatttac atccgtaccg cttaccagag gatgcaacag ttccttctct
     5461 aattcccatt cctgattaag agccaaggat tttagagtca gcgtagaacc attatctctt
     5521 atcaaattca tgataaatac tttatcggcg ctggtctgta accccacaaa tatatcggct
     5581 atatgatcaa gtttatttgg taaggttctc attcgttcaa ataacgctct gctcggacca
     5641 gttacaaagt tccattcagc cgcgctgatc aagtcggaag gtagaatatt tcgtttcttt
     5701 ttaaggccaa tgttagatcg ccacatttca acatccatca ccttttcgaa gttgaaattc
     5761 ctctgaatct gcttttccag aaagaacaaa catgtgtaag ttgtagcatt gacaaatatc
     5821 tgtttgtctc caaaatgaat tatttcagag agatgtttgc catcggaaat caatttgcgt
     5881 aatggttcac cgtattgagc attaaaaaat ttgtgaggca gaataaaccc aagacgaccg
     5941 gttttatcga gtagctgcaa tcctttttct acaaagacaa cataaatatc ataattgcct
     6001 ttctgtgctg attgataagc ttccttataa aactcgacct ctttaggtgc ccattctttc
     6061 aatgcctgaa tccggatgta cggcggattg ccgattactg cgtcgaagcc gcctgctttg
     6121 aagatgtccg gaaactcggc ctgccagtca aaggcgttga cgcgctgcat ctcttccgta
     6181 tccagcaggc tcgtttgctt gccatcgtaa aaatccgggc cgatgagcga attgccgcac
     6241 ttgatgttgc cgcccagatt cggcaacgcc cgttcgtgaa agagcttgaa ctgataattg
     6301 agcgtttcgc cggtttcgcc ttccagaact ttgagcagaa gtgaaagctt ggtgacctcg
     6361 caggcctgaa aatcgatgtc cacaccgtag atgttgttga gcaaaatgcg cttgcgttcg
     6421 gcggtggtca gatgccacgc tccgccttgc cctttgaaga gaaccggctt tttggctctg
     6481 gcgcattttt cgggatcgtt ttccgcatac cactttacat gccagtccag cagatgctga
     6541 tacgcgccca gcagaaagga cccggacccg caggcgggat caagaatgcg cagtttctcc
     6601 gctgtcttgg gtgtttttcc ttcaaggagt ttgcccacgg tgttttgaac gatatagtcc
     6661 acaatgtagg tgggcgtgta gaaaacgccg cccgcctttt tgacttccgg cttgtcttcg
     6721 accacggcgc gatggtcgct ggtgagacgg atgaccttgc ccaggaactg ttcataaacc
     6781 tggcctaaaa tgtccgcggg caaaacggaa aattcataag gcgaatctgg atagtaaagg
     6841 cgtttgagaa tgtccttgag cggtttgtcg tcaatggtaa gcgacgtcgt gagattgtcg
     6901 ggcggttccg cctcgttttt ttcatgacgg aaatgaaaca ggccggagtt atagcggtca
     6961 tcggctttgt agaacagttc ccgcagcctg gcgtaagcat tattgccgtt gcagaggccg
     7021 cgcagactgc cgtattcctc aatgccccgg tcttccgcga tgcgcaggaa aataatcctg
     7081 tcgatggtgc gctgaacggc gtcgttcaat tccctgacgc tcagtgtgtt gcgaagtgcg
     7141 atgttgcggg ccagcatttc gcgccagctt tcgatctccg ccagaaaagc gctgtccact
     7201 tcggcggtgc cccgtttgcc tttgttaaat tcggcgtatt tgtcaaacga gcctttgaga
     7261 atggcgtctt tggaaaagat gccggcgatt tcatcccatt tttcgtcgta ctgtttgtag
     7321 gtatagtaaa ggatgcgggc cttgtccgcc ctgtcggatt ggtccggctt gacgcggcag
     7381 tcgtaaacgg caaattcgtc aaagtcggtg acaatggaaa gcggcagctt tgccgaccag
     7441 gcataacggc gaacctgata agcgggcgcg ggcgtgtcct tgatgttgat ggagggctgc
     7501 ttggcttcga caaagaactt gcgctgtccg ccgatgcgaa aggaataatc cggcgctttg
     7561 gtgacgccgc cgactttcac ggcgtcttca tgaatgacgt ctttataggc ttcggcattg
     7621 cccgcttcat tgttgacatc ccagcccagc gcttggaaaa atggatcgat aaattcccgg
     7681 cgcacctgtg tttcgttgta ggcgccggat ttgtaggctt ccttgtttct ttcaaaccgt
     7741 tctatgagat caagaataat ttgcggagcg ggcatggaag ttccttacat ttcgacaaga
     7801 aatatttgta gaactttctg acgttgcttc atatagccaa ttgaaattct tatcaataat
     7861 tttcttgcgg atgcgctttt tttattttca catttccgac gttgcatgct attaaacgca
     7921 aaaaataagg acattgccat gaagaaagcg cctcttgtgt tcggcatcgg ccagtgctcg
     7981 ctggatcatc ttggcctggt cccggtttat ccccctccgg acgtgaaatg cgaatttacc
     8041 aacctggttc tgcagggcgg cggccccgcc gccacggcgc tggtcgcgct gaaacgctgg
     8101 ggcgcccgct gccacatcgc cggtgtgctg ggtgatgatg gtttcggaac gcagatccag
     8161 gcgctgcttt ccgccgaaga catagacacg acaggcctgc aaatccgcaa acagcatcag
     8221 tcacagttcg cgtttatcgc cagcgagcct gcgctggccc ggcggacgat cttttaccag
     8281 atgcccacgg gcgcgccgct tcggccggag gaaatcaaca ccggcattct gcttaagagc
     8341 caggcgctgc ataccgacgg gctgtttacc gaagcctcgc ttttcgcctg gcgaaaatga
     8401 gcgactgctt tgtcacatcg gaagtattct ccaacgcttt tgcagaaacg cccgccgaaa
     8461 cctgccgaaa gcttgccgcc atgggcgttc gcctgccggg cgtcacgctg ggcaagcggg
     8521 ggtatgtggc gctggttgac ggacgcttca tcgaaaaaga agcctatccg gccaaggcga
     8581 tcgacacgac gggctgcggc gacgttttcc acgcgggctt gatccacgga attcttcatg
     8641 gatggagcgc ggaaaaatgc ctcgacctgg gcgcctgggc ggccgcgcgc gtcagcgaac
     8701 aaatgggcgg acgcagcggc attcccgata tccgcactct gcagtccaaa tatcccggcg
     8761 accacggccg gtaaaagagc ctgaaaatca ggacgagacc gccgcaaacg caaaaatgac
     8821 aagcacggga ttttctttga cagagccgaa gggtttcact atactcgggc gcgaaaataa
     8881 tctgttttct gaacgtgcaa catcgtttgt caaaaggccg gtttaagtta tggccgggac
     8941 atcttgcaat atttccggag aggagaaggc ttatgtcgga ttatgaagtc gtggtaatag
     9001 gagccggcaa tggcggactg acggccgcgg ccgggcttgc caagcgcggg cacaaaacgc
     9061 tgctgcttga aagacacaat gtcccgggcg gatgcgcgac aagcttcgtg agaggcagat
     9121 tcgagttcga agtcgcgctt caccagttga gcggcatggg gctgcccgaa tttcccgggc
     9181 cgctgagggg actgctcaac gatctgggcg tgatggacaa aatcgagttt gtccagcaga
     9241 aaaatcttta ccgcgtggtc gtccccggac aactggacat caatctgaaa gccgaccggg
     9301 cggcggtcat cgaatctctg acggaaagat ttccggccga aaaggagggc atccaaaagt
     9361 tctttgacct tctctacgcg ttctgcaacg aactggtgaa cgtcctgttc atccatgatc
     9421 cggaggccag ccccgaaaaa taccctcttt tcttcaacct cgcgctgaaa aactcgcagg
     9481 gcattctgga cgactacctc aaggaccgac tgctgcaggc cgctgtgacc atctactgga
     9541 gctacgcggg gcagccgccg agccggctga gttttatgga gctggcgatg atgatctggg
     9601 cgtatatcga gtttaagccc tggcacatga agggcggatc gcaggccatg tccaacgcta
     9661 tttgcgacgc ctttctccgc tgcggcgggg atgcgcgatt caactgcgcc gcccgaaaaa
     9721 tcctggtcaa aaatggaaga gtagctgccg tcgtgacgga ggatggagaa gaaattgcga
     9781 caaagttcgt ggtgtccaac gccggtccga ttcgcacgct catcgacatg gtcggggcg
//
LOCUS       DAOSIM010000051         9836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_6663_length_9836_cov_7.516921, whole genome shotgun sequence.
ACCESSION   DAOSIM010000051 DAOSIM010000000
VERSION     DAOSIM010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9836
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6663_length_9836_cov_7.516921"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1980)
                     /locus_tag="PLR47_03720"
     CDS             complement(<1..1980)
                     /locus_tag="PLR47_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOG12078.1"
                     /translation="MKPETIDPSGHKSKNRIWLFSLCVIAFLIAASDLQAGPAVPDGR
                     PRIGLVLSGGGARGLAHVGVIEVLEEMKIPIDCIAGTSMGAIVGGIYASGMSPDELKK
                     FITSMQWNEAFKDKPDPSEMTFRRKRDAADFLIDLDLGFKDGEFAMPRGLLQGQNLNL
                     ILKSLLIHTEGIDDFNRLNIPFRAVATDIETGDAVILEKGELVKAIRASMSIPALFAP
                     VEIDGKLLVDGGVANNLPIDIARQMGADVVIVVDISTELSRRDQLDTSVGITSQLTSI
                     MVQRNTQAQIRTMREGDILIRPELGTIGSSDFFKAADAVAAGRKKAAEMKPELAHLAV
                     REEDFPAYLRAQRKKDQAMPVIDVVEVDNKSSFPDGLIAAQVNIKPGEPLNMPELKKN
                     IDRVYGIDTFERVDFHLRKKDKSTAIVIEPFDKAWGPNYFRFGLGLEDNFKGSGSYAL
                     TAQFTKTALNRLAGEWRTELRIGENPRIYTELYQPLDYSLSYFAAANAAYLMRNINTY
                     NDKGEILTQYRVQSAQFGVDAGRQFGNWGQVRAGLRREHGNVKIAIGDPSVPEETYNR
                     GSVQASVAYSRLDNYVFPLRGTDASLMWNYDLKALGSDIDEQALGFNWMTAFTWGKYT
                     FIPSLDVKTMLDDDSVAVQDTFPLGGFLNLSGFSTN"
     gene            complement(2006..2590)
                     /locus_tag="PLR47_03725"
     CDS             complement(2006..2590)
                     /locus_tag="PLR47_03725"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOG12079.1"
                     /translation="MFYQIIKAGNDEIGLVWEDAGGKTQVECIYLPGREKIIQKIFRD
                     FPAVEKKPRKISGGLDRLIVDLCLGKERRVDLSFLNLSGLTEFSGRVLRQTHRIPRGK
                     VCTYSGLAARVGFPRAARAAGSVMANNPFPLVIPCHRVIRADGTPGRFGGGADMKRRL
                     LEREGIFFDAKGRVPSEYRVTAKRRRRDDAGDHS"
     gene            complement(2747..3304)
                     /locus_tag="PLR47_03730"
     CDS             complement(2747..3304)
                     /locus_tag="PLR47_03730"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOG12080.1"
                     /translation="MSLITIFLLAVGLGLDAFSVAIAIGAANAKKSWAPVLRLSLAFG
                     LFQFFMPLAGWLAGSTVVDRIQAFDHWIAFALLVLVGGKMIREGFEKEKDEEKADQTL
                     GWPLFLLSIATSIDALAVGFSFSLLKTPILFPAAIIGMVCFVMTAVGMVFGKGLARIF
                     GRKVEILGGLVLIAIGVKILIEHMG"
     gene            complement(3332..5179)
                     /gene="uvrC"
                     /locus_tag="PLR47_03735"
     CDS             complement(3332..5179)
                     /gene="uvrC"
                     /locus_tag="PLR47_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOG12081.1"
                     /translation="MDENAAVKEKMASAPRAPGIYIMLDARRKVIYVGKANDLKSRVG
                     SYLTGRDTRPMAPFLMARVQSIEFITTDTEKEALILENNLIKKHRPRYNVILRDDKTY
                     CHLSLNPSEAFPRLRLVRKRQNDAALYFGPYPSGLAARETLRFIQRIFPLRTCRDRDF
                     QLRARPCLEFQIGRCLAPCKNRIDEAAYRTLVENAVSFLRGRRRELISDLKKQMSEAA
                     QSLRYEDAARLRDRIGALEHALERQHVDAGGAADQDVLGVHSDSGTHRVCILFVREGK
                     LLGSKSFTPVKTKADREEILSSALRQYYDEAGLPEEILLPFSLSDEAVLLEWLAEKRQ
                     RSVRITVPQRGAKKALVDMACANARELQASDRKKDEQKTAVLQFLRERLSLAGLPRRI
                     ECFDISNLGGKNAVGSMVVFQDGEPDKSAYRRFRVRGPDEPDDYGMMREVLTRRFQGR
                     DPVPDLLVVDGGKGQLNVALAVLSDLKIKLDVIGLAKEERSFLAAKRRLQGKPAKSED
                     RVYLPRRKDAIYLSSSPAALSLLQRLRDEAHRFAVSYHRKLKQKNDFRSALDDIPRLG
                     SRRKSILLRHFGSLERIRSCSPEELQALPGIGRALAETIHKVLSSGGAA"
     gene            complement(5200..6036)
                     /locus_tag="PLR47_03740"
     CDS             complement(5200..6036)
                     /locus_tag="PLR47_03740"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOG12082.1"
                     /translation="MAIPYHALTYPDDRRTSREAMGCPAFAVIAHRGASACCPENTLS
                     AFRAAIEMKADMVELDVQLTADREVVVFHDAKLSRLTDGRGMVADYPFARLRQLDAGV
                     WFHKKYAGERIPALVEVLALCKGRMAVNIEVKTEAVTDAVSGGIEERCLKMVDQIGMK
                     EHVVYSSFDPRAILHFKQIDREAPVAVLYEKKQHGSRLPSEIVLSLGVDAFNCSRREL
                     NGTWLADLKRHGIPVHIYTVNDEKNMRRLLEMGVDGIFTNRPDVLRNVCEEYKGRTRD
                     EG"
     gene            6188..6763
                     /locus_tag="PLR47_03745"
     CDS             6188..6763
                     /locus_tag="PLR47_03745"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOG12083.1"
                     /translation="MKVLGIMGSARVGGNSDVLLSRALEGAREAGARVQKIVLDRKHI
                     AGCRDCKKCNKTGICVLADDMSGIQELILEADAVLHAVPVYFWSMSSQMKAYLDRWCV
                     FFDAEWRWQKACYPKMRGKRIGLLTVCGDPDPHTADPIVHSFRMTAEMTKMHWLGAVM
                     ASASDKGEIKGNGAALREAFDLGKKSAAPQQ"
     gene            complement(6892..7941)
                     /locus_tag="PLR47_03750"
     CDS             complement(6892..7941)
                     /locus_tag="PLR47_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOG12084.1"
                     /translation="MKKSSASLPVHPKKKKLNLALQGGGSHGAFTWGVLDRLLDEKRL
                     EIEGIVGTSAGAVNATILAYGLAAGGPAKAKELLNRFWMTNSKNSRCSPFQPTVFDKL
                     MGSKGSIEFSPYWQMFDILSRMFSPYQWNPNNKNALKEMLEELIDFSVLQKSRSIRLF
                     ICATNVLTGRLKVFENREITPDMIMASACLPYLTQAPEIDGQYYWDGGYMGNPPIFPV
                     IYDTDCRDVLIVQINPINIPEVPRSTNEIFDRINTLSFNSSLMREMRAIYFITSLIEK
                     GELDSAKHKHTFIHTIDAEEEMAKLSASSKMNLDVEFLQYLFDTGREKADEFLKNHFD
                     KIGRRSSTDLVAKFF"
     gene            complement(8184..8789)
                     /locus_tag="PLR47_03755"
     CDS             complement(8184..8789)
                     /locus_tag="PLR47_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12085.1"
                     /translation="MTKRLSGILVMIVVMTFHSAVSSALDKAQKFKEYASFGNYFQCS
                     IPEDWSVYPPGFGLSEEEKKVYGVILIGPRNTSPVAPLMSIHYYAPGNLLHSTMDIFI
                     RRHSRSNLGIEIEGKSYGKIQEMEVAGRQAKTFERIDIRFIGERVINPTKVPIYEKFV
                     TIPAGSGEGFYVLKLSVPAEMKDAYKGLFEVTVKSFLPKNE"
     gene            complement(8898..>9836)
                     /locus_tag="PLR47_03760"
     CDS             complement(8898..>9836)
                     /locus_tag="PLR47_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG12086.1"
                     /translation="YYFRARISEKNQAMSDYYKILSIEPKDSEAHLIRAWTYKELDQR
                     DPALQEFGKAIAKARGNKEKAVAYFDRGRYYTLFRSRPYVAPSKQDIPGALELSVRDF
                     SSAIALDPADSSYFHNRANSYLGLNQYDLAIEDFNAAIRLDPKSAGLFSGRGQTYQLL
                     QKPELAVADLSRAIELEGPDHLFAAHDYMLRAMSYEKLGKFDLAIQDWTNLLKRKPDD
                     PFYLRERADLYSKIGKYDQAIKDYGRSIALNPREAAAAYYGRALAYALKGDSRKSIQD
                     LRNAIQLDPGYKANVRNEAGFNSLQHHPDFIKLIGK"
BASE COUNT         2163 a         2852 c         2540 g         2281 t
ORIGIN      
        1 attggtggaa aagccggaca gattcaggaa gccgcccagc ggaaacgtgt cctgtacggc
       61 cacgctgtcg tcgtccagca tggttttgac gtcaagggag ggaatgaagg tgtacttccc
      121 ccacgtgaag gcggtcatcc agttgaagcc cagggcctgt tcatcgatgt ccgaacccag
      181 cgctttcaaa tcgtaattcc acatcagact ggcgtccgtt cccctgagcg gaaaaacata
      241 gttgtccagt ctgctgtagg caacggaagc ctgaacgctg ccgcggttgt aggtttcttc
      301 cggcacgctg gggtcgccga tggcaatctt cacgttgccg tgctcccggc gaaggcccgc
      361 gcgcacctgg ccccagttgc cgaactgcct gcccgcatcc acgccgaatt gagcggactg
      421 gacgcggtac tgcgtcagga tttcgccctt gtcgttgtac gtattgatgt tgcgcatgag
      481 ataggcggca ttggcggcgg caaaatagct gagtgaatag tccagcggct ggtaaagttc
      541 ggtatagatg cgcgggttct cgccgattct caattcggtg cgccattctc cggccagtcg
      601 gttcagagcc gtcttggtaa actgcgcggt cagggcataa ctgccggagc ctttgaagtt
      661 gtcctccagg cccaggccga accggaaata attcggaccc catgctttgt cgaacggttc
      721 gatgacaatc gccgtggact tgtctttttt ccgaaggtgg aagtccacac gttcaaaggt
      781 gtcaatgccg tacacccggt cgatattttt tttcaattcg ggcatgttca ggggctcacc
      841 cggtttgatg ttcacttgcg cagctatcag accatcggga aaagacgact tgttgtccac
      901 ctccacaacg tcgatcaccg gcatcgcctg gtcttttttc ctctgcgccc gcagataggc
      961 cggaaaatcc tcttcccgca cggccagatg agcgagctcg ggcttcatct ccgcggcctt
     1021 cttccggccg gcggcgaccg cgtctgccgc cttgaagaaa tcactgctgc cgatcgtacc
     1081 caactccgga cgaatcagga tgtccccttc ccgcatggtg cggatctgcg cctgggtgtt
     1141 gcgctgaacc atgatggagg tcaactgcga ggtgattcca acggaggtgt ccaactgatc
     1201 gcggcggctc aattccgtgc tgatatccac cacaattacg acatccgcgc ccatttgccg
     1261 cgcgatatcg atcggcagat tgttggcgac gccgccgtcc accagaagct tgccgtctat
     1321 ttccaccggg gcgaacaggg cgggaatgga catgctggcg cgaatcgcct tcaccagttc
     1381 ccctttttcc agaatgacgg cgtcgccggt ttcgatatcc gtggccacgg cccgaaacgg
     1441 aatattcagc cggttgaaat cgtcaatgcc ttcggtgtga atcagcaggg atttcagaat
     1501 caggttcaga ttctgcccct gaagcagccc tctgggcatg gcaaattcac cgtctttgaa
     1561 gcccaggtcg aggtcgatca gaaagtcggc cgcatctctc ttccggcgga aggtcatttc
     1621 gctcgggtcc ggcttatcct tgaaggcttc attccactgc atgctggtga tgaatttttt
     1681 cagctcgtcg ggcgacatgc ccgacgcgta aattccgccg acaatcgcgc ccatgctggt
     1741 tcccgcgatg cagtcaatcg gaattttcat ttcttccagg acctcgatca cgcccacgtg
     1801 cgccagcccg cgcgcaccgc ctccgctcag caccaggccg atccggggac gcccgtctgg
     1861 aacggccggc ccggcctgaa ggtcggacgc cgcaatcaga aaagcgatga cgcacaggct
     1921 gaaaagccaa atccggtttt tgctcttatg gccggaagga tcaatggttt ccggtttcat
     1981 atccgttccc tctaatgtcc attgtttaag aatgatcacc tgcgtcatcc cgccgccgcc
     2041 gctttgccgt tacacggtat tccgaaggca ctcttccttt cgcatcaaaa aaaattcctt
     2101 cccgttccag caggcgcctt ttcatgtcgg cgccgccgcc aaaacgcccc ggcgtcccgt
     2161 cggcccgaat cacgcgatgg cagggaatca caagcggaaa aggattgttc gccatcacgg
     2221 aacccgccgc ccgggccgcg cgcggaaatc cgaccctggc cgccagaccg gaatacgtgc
     2281 acactttccc gcgagggatg cgatgagttt gccttaacac ccgccccgaa aattccgtca
     2341 acccggataa attcagaaag gataaatcca cgcgccgttc ttttccaagg cacaaatcaa
     2401 caatcagacg atccaggccg ccggatattt tgcgcggctt tttctcgaca gcgggaaaat
     2461 cccgaaagat tttttggata atcttttccc tgcccggaag ataaatacac tcaacttgtg
     2521 tttttccccc ggcatcttcc cacaccaatc cgatttcgtc attcccggcc ttgatgattt
     2581 ggtaaaacat cagacacctc gtatagattt tcaggctgaa ggctgaagac cgaaggtcaa
     2641 ttttgttgac cttcagcatt tttatttttt ctcgcgcctt tgctgtgaaa caaaaacgtt
     2701 cggtcctttt tccacgtctt ttccttcagt cttcagtctc cggtcttcat cccatatgtt
     2761 cgatcaatat cttcacacca atggcaatca aaaccagacc gcccagaatc tctactttcc
     2821 tgccgaaaat ccgcgccaat cccttgccga aaaccatgcc caccgccgtc atcacaaaac
     2881 aaaccatccc gatgatcgcc gccggaaaca ggatcggggt ctttaaaaga gaaaaactga
     2941 agccgaccgc cagcgcgtca atgctggtcg ctatcgaaag cagaaagagc ggccagccga
     3001 gcgtctggtc ggccttttcc tcatcctttt ccttttcaaa accctcccgg atcattttcc
     3061 cgccgaccaa aaccaaaagc gcaaaagcaa tccagtgatc aaaagcctga atcctatcga
     3121 ccacggttga accggccagc catccggcaa gcggcatgaa gaactgaaaa aggccgaaag
     3181 ccagggaaag ccgcagcacc ggcgcccagg atttttttgc attggccgcg ccgatggcga
     3241 tggccacgga aaacgcgtca agtccaaggc caaccgcgag aagaaaaatc gtaatcaagc
     3301 tcatatcgca ccgaactctc ctggacaggc tttaagctgc tccacccgaa gacaatactt
     3361 tgtgaatagt ttcggccagg gccctgccga tgcccggcaa cgcctgcaac tcctcgggtg
     3421 aacagcttcg aatccgctcc agcgacccga aatgccgcag caaaatactc ttccgacggc
     3481 tgccaaggcg gggaatgtcg tccagcgcgg agcggaagtc atttttttgt tttaatttcc
     3541 ggtgatagct cacggcaaag cggtgcgcct cgtcccgcag ccgctgcaaa agggacagag
     3601 cggcgggtga cgacgaaaga taaattgcgt ccttgcgccg gggcaaatag acgcgatcct
     3661 cggattttgc cggctttccc tgaagtcttc ttttggccgc cagaaaggac ctttcctcct
     3721 tggccaggcc gatgacgtcc agtttgatct tcaagtcgct caaaacggca agagcgacat
     3781 tgagctgtcc tttgccgccg tcaaccacca gcaggtcagg caccgggtcc cgaccctgga
     3841 aacggcgcgt caacacctcc cgcatcatcc cgtaatcgtc cggctcatcc ggaccgcgaa
     3901 cgcgaaagcg ccggtaggct gatttatccg gctccccgtc ctgaaacacc accatcgacc
     3961 ccacggcgtt tttcccgccc aggttggaga tgtcgaaaca ttcgatccgg cgcggcagac
     4021 cggcaagcga aagcctttcc cgcaggaatt gcaaaacggc tgttttctgt tcgtcctttt
     4081 tccggtcgga cgcctgcagc tcgcgggcat tggcgcacgc catgtcaacc agcgcctttt
     4141 tcgcgccccg ctggggcacg gtgatgcgca cgcttctttg ccgtttttcg gccagccatt
     4201 cgagcaaaac agcctcatcg gacaggctga agggaagaag aatctcttcc ggcagaccgg
     4261 cttcgtcata atactgccgc agcgccgatg agagaatttc ttcccggtcc gcctttgttt
     4321 ttaccggcgt aaaggatttg ctgcccagca gctttccttc gcgcacaaac agaatgcaga
     4381 cccggtgcgt tccggaatcg gaatgaacac ccagcacatc ctggtccgcg gcgccccccg
     4441 cgtccacatg ctgccgctcc agcgcgtgct ccagcgcgcc aatgcggtcg cgcagcctcg
     4501 cggcgtcctc gtagcgcaag ctctgcgccg cttcactcat ctgctttttc aaatcggaaa
     4561 tcaattcgcg gcggcgaccc cgcagaaaag atacggcgtt ttccaccagc gtccggtatg
     4621 ccgcttcgtc aatccggttt ttgcagggag ccaggcaacg gccgatttga aattccaggc
     4681 agggtctggc ccggagttgg aaatcccggt cccggcacgt gcgcagagga aagatgcgct
     4741 ggatgaaacg gagcgtctcc ctggcggcca gccccgacgg gtacggaccg aagtaaagcg
     4801 ccgcgtcatt ttgccttttg cgcaccagcc gaaggcgcgg aaaagcctcc gaaggattca
     4861 gcgacagatg gcaataggtt ttgtcatccc gcaggatgac attgtagcgc gggcgatgct
     4921 tcttaatgag gttgttttcc agaatcagcg cttctttttc cgtgtccgtt gtgatgaatt
     4981 cgatcgactg aacgcgcgcc atcaaaaacg gcgccatcgg cctagtgtcc ctgcctgtca
     5041 aataagaacc gacgcggctt ttcagatcgt tggccttgcc cacatagatg actttccgtc
     5101 gcgcatccag catgatatag atgccgggcg cacgtggagc ggaagccatc ttttctttta
     5161 cagcggcatt ctcatccatc aatacggatt cctcacccct cacccctcat cccttgtccg
     5221 ccccttatat tcctcgcaga cgtttcgcag aacatccggc cggtttgtga aaatgccgtc
     5281 cacccccatc tccagcagcc gccgcatgtt cttttcgtcg ttaaccgtat agatatgcac
     5341 cggaatgcca tggcgtttca gatccgccag ccatgttccg tttagttcgc gccgcgaaca
     5401 gttgaaggcg tcaacgccaa gggatagaac aatctccgag ggcagccggg agccatgttg
     5461 ctttttttcg tacagcacgg caacaggcgc ctccctgtcg atctgcttga aatgcaaaat
     5521 ggcgcgggga tcaaagctgg aatacaccac atgctccttc atgccgatct gatcgaccat
     5581 tttcaggcat cgctcctcaa tacctccgga aacagcatcc gtgaccgcct ccgtcttcac
     5641 ctcgatgttg acggccatcc ggcccttgca gagcgccagg acttccacca gcgcaggaat
     5701 gcgctctccc gcgtattttt tgtgaaacca gacgccggca tcgagttggc gcaggcgggc
     5761 aaacgggtag tcggcgacca ttccccggcc gtccgttaag cgcgataatt ttgcatcgtg
     5821 aaagaccacc acttccctgt ccgcggtgag ctgcacatcc agttccacca tgtcggcctt
     5881 catctctatt gccgcccgga aagcggataa cgtgttttcc ggacagcagg ccgacgcccc
     5941 gcggtgggca atgacggcaa acgcggggca tcccatcgct tcccggctgg ttcgccggtc
     6001 atcaggatag gtcagggcat gatagggtat ggccatcggc agcaccgctt agcttttcag
     6061 tgtccggttt tctaacatat ttgaccgcag agttcttcaa ctattttatc gtttacaaaa
     6121 tcccggagaa aaggtacaaa gcgagcaagg attcaggggt cccattccga caaaccggga
     6181 ggaaaacatg aaagtacttg gcattatggg aagtgcgcgc gtcgggggaa acagtgatgt
     6241 tctcctgagc cgcgctctgg agggcgcccg ggaagccgga gcgcgcgtgc aaaaaatcgt
     6301 cctggaccga aaacacattg ccggatgccg agactgcaag aaatgcaaca aaaccggaat
     6361 ctgcgtcctt gcagacgaca tgtccggcat tcaggagctg attctggagg ctgacgcggt
     6421 ccttcacgcc gttcccgttt atttctggtc gatgagctcg cagatgaagg cctacctgga
     6481 ccggtggtgc gttttttttg acgccgagtg gagatggcag aaagcctgct accccaaaat
     6541 gcggggcaag cggatcgggc ttctcactgt ttgcggggac ccggacccgc acacggccga
     6601 ccccattgtc cacagcttcc gaatgaccgc ggaaatgacc aaaatgcact ggctgggcgc
     6661 cgtgatggcc tccgcatccg acaagggcga aatcaagggc aacggggccg ccctgcggga
     6721 ggcgtttgat ctggggaaaa aatccgccgc gccgcagcag tgatttttca agcccgaagc
     6781 ccgcgcgtca gccttcgcat tcggccggaa tgaggcggat gcagaaaaag ctccccttcc
     6841 ccgcgggccg aagattctcc gcttgcggcc tcgaagacct tcccacgcgg atcagaagaa
     6901 tttggccacc agatcggtgg acgaccggcg gccgattttg tcgaaatgat ttttgaggaa
     6961 ttcatcggcc ttttcccggc cggtatcaaa caggtattgc agaaattcca catccagatt
     7021 cattttgctc gacgcggaga gttttgccat ttcttcctcg gcgtcgatgg tatggatgaa
     7081 ggtatgtttg tgtttggccg aatccagctc gcccttttca atcaaagacg taataaaata
     7141 aatagcccgc atttcccgca tcaggctgga attgaagctg agcgtgttga tccggtcgaa
     7201 gatttcgttg gtgctgcggg gaacctcggg aatgttgatc gggttgatct ggacgatcag
     7261 gacgtcgcgg cagtccgtgt cgtaaatcac gggaaaaatc ggcggattcc ccatgtagcc
     7321 gccgtcccag taatactggc cgtcgatctc cggcgcctgc gtcaggtagg gcaggcaggc
     7381 ggaggccatg atcatgtccg gcgtgatttc gcgattttca aaaaccttca ggcggccggt
     7441 caaaacattg gtggcgcaga tgaaaagccg aatgctgcgc gacttctgca gcacggaaaa
     7501 gtcgatcagc tcttcgagca tctccttgag ggcgtttttg ttgttggggt tccactggta
     7561 gggggaaaac atgcgcgaaa gaatgtcgaa catctgccag tacggagaaa attcaatcga
     7621 ccctttgctg cccatgagtt tgtcgaatac ggtcggctgg aacggcgaac accggctgtt
     7681 ctttgagttc gtcatccaga aacggttgag cagctccttc gctttggcgg ggcctcccgc
     7741 cgccaggcca taggcgagaa tggtcgcgtt tacggcgccg gcgctcgtgc cgacaatgcc
     7801 ttcaatctcc aggcgctttt cgtccagcag ccggtccagg accccccagg taaaggcgcc
     7861 gtgggagccg ccgccctgca gggctaaatt cagttttttc tttttgggat gaaccggcaa
     7921 gctcgctgac gatttcttca ttcgatttta cccctgatcc acgatctaaa agaaaagaca
     7981 ttcaagcttt ttaagggcct taccaattcc gcatccgctt ccattgttca aaacagcgca
     8041 aaaacatggt atccgtataa cacattttgg aataagatca atgattattg cggcttaaat
     8101 tattatgaca cgccatccgc cacgcgtttg gtatttcaac attcagttcg ggagtctttg
     8161 ttgatttgca attgtcgggt ctcttattca tttttcggca ggaaggattt taccgtaacc
     8221 tcgaagagtc ctttgtatgc atctttcatc tctgcgggga cagacaactt cagaacataa
     8281 aatccctcgc cgcttccggc gggaatggtg acaaattttt cataaatcgg caccttcgtc
     8341 gggttgataa cccgctctcc gatgaaccgg atgtctattc tctcaaatgt cttcgcctgt
     8401 cttcccgcaa cctccatttc ctgaatttta ccgtaggatt ttccctctat ttcaatgccc
     8461 aaattggatc tggagtgcct ccggataaat atatccattg tgctgtgcaa aagattcccc
     8521 ggagcataat agtgaatgga cattaaggga gcaaccggac ttgtgtttcg cggacctatc
     8581 aggataacgc catatacttt tttttcttcc tcggataagc caaagccagg gggataaacg
     8641 ctccagtctt cgggaatgct gcattggaag taatttccaa aagacgcata ttctttgaat
     8701 ttttgtgctt tatccagcgc gctggaaact gcggaatgaa aggtcatcac gacaatcatt
     8761 acaagaatcc ctgataatcg tttcgtcata aaacctcctt aaatcaataa gcccggtcgt
     8821 tgatcggact tttctttaac attcctgttg ctcttcaccg gctgatattc attcacttaa
     8881 aacaaaaccc ctgcggctca ttttccaatc agcttgataa aatcaggatg atgttgaagg
     8941 gaattgaatc ccgcctcgtt gcgaacattc gccttataac cggggtcgag ttgaatcgca
     9001 tttctcaagt cctgaatgct cttgcgtgaa tcccccttca acgcataggc cagagcgcgg
     9061 ccataatagg ccgccgcggc ctcccgggga ttaagcgcga tggatctgcc gtagtctttt
     9121 atcgcctgat cgtacttgcc gattttgctg taaaggtcgg ctctttctct gaggtaaaac
     9181 ggatcgtctg gttttcgttt aagcaagtta gtccaatcct gaattgccag atcaaacttt
     9241 ccaagcttct catagctcat cgctctcagc atataatcgt gagcggcaaa aagatggtcc
     9301 ggtccttcca gttcgatcgc acggctcaaa tcagcaaccg ccagttccgg tttctgaagc
     9361 agttggtagg tctgtccccg gccggaaaat agtcctgcgc ttttcgggtc gagcctgatc
     9421 gctgcgttaa aatcctcaat cgcaagatca tactgattga ggcccagata agaatttgcg
     9481 cgattatgaa aataagatga gtccgccgga tcgagtgcga tggcgctgct gaaatcccgg
     9541 accgagagct caagcgcgcc ggggatatcc tgtttggacg gtgctacgta aggtctggag
     9601 cgaaacagcg tgtaatatct cccacggtcg aaataagcga ctgccttttc tttgtttcct
     9661 cgcgcctttg cgatcgcttt cccgaactcc tgaagcgcgg gatcacgctg atcaagctcc
     9721 ttgtatgtcc aggcccggat gagatgcgcc tcggaatcct tcggttcgat gctcaatatt
     9781 ttgtagtaat cggacatggc ctgatttttt tcactgatcc gcgcccggaa ataata
//
LOCUS       DAOSIM010000052         9736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_6748_length_9736_cov_9.438591, whole genome shotgun sequence.
ACCESSION   DAOSIM010000052 DAOSIM010000000
VERSION     DAOSIM010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9736
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6748_length_9736_cov_9.438591"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PLR47_03765"
     CDS             complement(<1..665)
                     /locus_tag="PLR47_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12087.1"
                     /translation="MELIFEISKSGRSAVQVPASDVPEVNIGDAIGPKYLRGDLDLPE
                     VAEIDLVRHYTNLSRRNFGVDLGFYPLGSCTMKYNPKINENIAALPGFAGLHPNAPSE
                     FAQGSLGLMYDMQRLLAGLFGMADFTLQPAAGAHGELTGVMMIRKYFEKRGEKRRRIL
                     IPDAAHGTNPASGALCGFETVTLRSNPEGGVDLNHLESLMTQDTAALMLTNPNTLGLF
                     ERN"
     gene            complement(742..2067)
                     /gene="gcvPA"
                     /locus_tag="PLR47_03770"
     CDS             complement(742..2067)
                     /gene="gcvPA"
                     /locus_tag="PLR47_03770"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOG12088.1"
                     /translation="MDYCPHTPDDIAQMQAGIGIGNIEELFADIPQKFRLRKIPGMPA
                     SLSEQEIWALMRTQSEKNRMPRATLTGAGAYHHFIPAVVNHIVGRAEFYTAYTPYQAE
                     ISQGILQAIYEYQTMIAHLTGTEIANASMYDGASAMAEAAVLCAKISNRSKLIAARSV
                     HPQYRRVLQTYAWANGCTLTEIPWLPSGQADKAALAAALDKETAAVIVQSPNFFGCIE
                     DLQPLADTAHDEGAMLIAGFADATSLGLLKPAGECGADFVVGEGQSFGNPLAFGGPYL
                     GIFAAREKYLRRIPGRLAGATVDKNGRRGFVLTLQTREQHIRREKATSNICSNEALCA
                     LAAGVYLAALGKNLGKLASLNVYKTQYLKNKLLQLRGWREIFSAPVYNEFALGCPDAG
                     RVNEKLRGEGIVGGYELSKDYPELDQALLFCATEMLSKDDMDNIVRILN"
     gene            complement(2072..2470)
                     /gene="gcvH"
                     /locus_tag="PLR47_03775"
     CDS             complement(2072..2470)
                     /gene="gcvH"
                     /locus_tag="PLR47_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOG12089.1"
                     /translation="MSKPNPTDRWYSKDHEWILDRGDGTASVGITDYAQEMLTDIVFV
                     ELPPIGKKVAAGEPLAVVESVKSVSDVYSPVSGEVIGVNTALENTPELVNRDAFGEGW
                     IARLKVADPEELKTLLNATAYDALIREEKH"
     gene            complement(2491..3582)
                     /gene="gcvT"
                     /locus_tag="PLR47_03780"
     CDS             complement(2491..3582)
                     /gene="gcvT"
                     /locus_tag="PLR47_03780"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOG12090.1"
                     /translation="MKKTPLYDRHAALNAKIIDFGGWAMPVQYTSVIEEHRATRANAA
                     LFDICHMGEIEVKGPQALDLLQFALTRDLAGQKTGQVKLSALLNDRGGIIDDLTVYKM
                     DREFYMLVTNATTREPDFQRIRSLLEEKKFDASLSDVSNSTGKLDLQGPRSEAILQTL
                     TPDDLKTIRFYWFIPSQVAGIPAIISRSGYTGEDGFEIYAAAEKIGAIWDRLMDAGAG
                     LGMKPAGLGARDTLRLESGMMLNGQDITQETCPLEVPYAWLVDWSKDFAGKTALSAVK
                     QAGVRKKLAGFEMTGRGIARHGYPVTKDGRAIGFVTSGTFSPTLNKAIGLAFIDAECA
                     SPEQAIAIGIRDAVSPAKIVPLPFYKRLK"
     gene            complement(3610..4071)
                     /locus_tag="PLR47_03785"
     CDS             complement(3610..4071)
                     /locus_tag="PLR47_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOG12091.1"
                     /translation="MAPKRVLTLWNDNGVNETDTSVLRKKSVEMKTPLDAAEKAEIKA
                     LVDSFTCRDDASGLAGPQIGVNKRIIIFRNKTGNDQKKKMSPEDYDVLVNPRITQARG
                     EKAAAAEGCLSCPDIQIDVERFPEIKVRALNEKGEKISPKSKQALIDKLFK"
     gene            4214..5473
                     /locus_tag="PLR47_03790"
     CDS             4214..5473
                     /locus_tag="PLR47_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase family protein"
                     /protein_id="HOG12092.1"
                     /translation="MINSIVASTAKKQFARALNFQEDDTTGLPACADNQRRLLYVHIP
                     FCEELCPYCSFHRVRLDEPLARRYHAALRREMALYRRQGYRFSGLYVGGGTPTILMDE
                     LAETLQIARDLFPIESVSVETNPNHLTDAHLSVLKRAGVHRLSVGVQTFDDGLLKKMA
                     RYDKYGSGEDIAARLSAAQGVFDTLNADMIFNFPEQTGQMLEADLAVLLKLRMQQITF
                     YPLMVSEFASRRMQKTLGDVNFRREPSFFRLIRRRLEPDYDSSSAWCFSRKKAAQSLI
                     DEYVVDFDEYAGLGSGAIGYLQGMCYANTFDIEKYIQDLSKDRLPIQASRRFDPPDRM
                     RYDFLMKLFSTRMNVEEMEKKYGGRFLKTLWKEMAAFTLAGSFRYFTPRLHLTPRGRY
                     LWVVMMREFFIAVNNFRDHCRREAGLG"
     gene            5635..7053
                     /gene="selA"
                     /locus_tag="PLR47_03795"
     CDS             5635..7053
                     /gene="selA"
                     /locus_tag="PLR47_03795"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOG12093.1"
                     /translation="MDDKRKALLKGLPKIDDVIGLLEKNGIYSKASKDIVREACREIV
                     QRLRDQILSAADKALPSLSFDAASAARAVEERMADLHRFKLRRLVNATGVILHTNLGR
                     APLCAEAVDRIVEVGRGYSNLEFDLIRGERGLRYDHVTGLICALTGAEDALIVNNNAA
                     AVLLTLNTLAEHKEAIVSRGELIEIGGEFRIPDVMTKSNSILREVGTTNRTRLADYEK
                     AIGPNTGVILKVHTSNYRIVGFTEEAELLPLVALGKHHGIPVFDDLGSGCLVDLAEFR
                     LQHEPTVREVLDSGVDVVTFSGDKLLGGPQAGIIVGKKDIVARIKKNPLNRALRIDKF
                     TLAALEATLMRYLAPGDAPRNLRSLRALTEPLGDVKKRARKLMAKLKRAGLSGLTYEL
                     KDSMAAAGGGSLPTEDIPSAVVTVKSAKMSASRMEASLRRAAVPVIVRVDEDEIQIDL
                     RTVAEDEFGFVVDALRGLADSP"
     gene            7274..8299
                     /locus_tag="PLR47_03800"
     CDS             7274..8299
                     /locus_tag="PLR47_03800"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOG12094.1"
                     /translation="MSIENQESQNLSFTVDSAQAGQRLDVFLSQADPAFSRSQIKYAV
                     EEGNVTVNGREPKASQHLKAGDRVELHLEPAVEAAAAPQDIPLNIVYEDGSIIVVNKP
                     AGMVVHPAPGNPDQTLVNALLFHCRDLSGIGGFLRPGIVHRLDKETSGLIVAAKSDEA
                     HRKLSAQFEKHDVHKKYIALCWGDVKGASGEIVLPVGRHPKDRKKMSTKSRHGKDALT
                     LWKVRERYGTATLLDIEIKTGRTHQIRVHLAERGYPVIGDMVYGGASKGQSVKNAALK
                     AGIKALSRQALHAAELSFLHPQSGERMVFTAPLPRDLEGLLDLFRSASPGGPGQTGLQ
                     TWKDALR"
     gene            8365..9219
                     /gene="pgeF"
                     /locus_tag="PLR47_03805"
     CDS             8365..9219
                     /gene="pgeF"
                     /locus_tag="PLR47_03805"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOG12095.1"
                     /translation="MFSWSNAKGIPYLQSSGLAACGFLEHAFCARKGGVSEDDYQSLN
                     MSFREGDEEFRVLQNWNKLAAAFGIPVENFLVLNQVHRDGILVIPSRGEYFTSREDLN
                     YDAIVTDRVHLAICIKTADCVPVFLADPENQVIAAVHAGWRGTAAGITAKVVRLMQNQ
                     YGSRPVDLLAAIGPSIGRCCYEVDAPAADAFRLHPDAKNFLFPSKRKDRWMLDLVQAN
                     RGQLIDCGIPDASIDASGLCTVCREDDFFSHRGSGGVTGRQINFLMLKGDPICKALLL
                     NDDLRHIH"
     gene            9248..>9736
                     /locus_tag="PLR47_03810"
     CDS             9248..>9736
                     /locus_tag="PLR47_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOG12096.1"
                     /translation="MNLKPLFEPKTMAVIGISASNDRHPANVIYDKNNHRYPVKVYAV
                     NPRGGSLHNETVYPRVAEIPAKIDLAVIATRAELVPDVMADCIAAGVKSATVISGGFS
                     EGGRGDLQDRIVDMAREAGLPFIGPNCLGVYVPFRVDTFFLPIERMIKPEAGGVTFVS
                     QSG"
BASE COUNT         1947 a         2807 c         2865 g         2117 t
ORIGIN      
        1 atgttgcgtt caaacaggcc cagcgtgttg gggttggtga gcatcagcgc ggcggtgtcc
       61 tgcgtcatca gggattccag atggttcagg tcaacgccgc cttccgggtt ggagcgcagc
      121 gtgacggttt caaacccgca aagcgcgccg gaggcgggat tggtcccgtg cgccgcgtcg
      181 gggatcagga ttcggcggcg cttttcgccg cgcttttcaa aatatttccg gatcatcatg
      241 acgccggtca gctcgccgtg cgcgccggcc gccggctgca gcgtgaagtc ggccatgccg
      301 aaaagaccgg cgagcagcct ctgcatgtcg tacatcagcc ccaggcttcc ctgcgcaaac
      361 tcagaaggcg cgttcgggtg caggccggca aagccgggaa gggccgcgat attttcattg
      421 atcttggggt tgtatttcat ggtgcaggag cccagcggat aaaagcccag atcgacgccg
      481 aagttccgcc tcgacagatt ggtgtaatgc cggaccagat cgatttccgc aacttccggc
      541 aggtccaggt cgccgcggag atattttggg ccgatggcgt cgccgatatt gacttccggc
      601 acgtcgcttg cgggaacctg cacggccgaa cgtccggatt tgctgatttc aaaaataagt
      661 tccatgaatg aactccttat tgccctgtca tttccgcgaa ggcgggaatc cagaaataat
      721 tcattttgcg cctgccatgg ctcaattcag tattctgaca atgttgtcca tgtcgtcttt
      781 ggacagcatt tccgtcgcgc agaagagcag cgcctggtcc agctccggat aatccttcga
      841 cagttcgtat ccgccgacaa tgccttcccc gcgcagcttc tcattgaccc ggccggcgtc
      901 aggacagccg agcgcgaatt cgttgtacac cggcgcggaa aaaatttccc gccagcctcg
      961 aagctgcaga agcttgtttt tcaggtactg tgttttgtaa acgttgagcg aagccagttt
     1021 gcccaggttt ttgcccagcg ccgccagata aacccctgcg gccagcgcgc agagcgcttc
     1081 gttggagcag atgttggacg ttgctttttc gcggcggatg tgctgttcgc gggtttgcag
     1141 cgtcagcacg aatccgcgcc ggccgttttt gtccaccgtc gcgccggcga gccggccggg
     1201 aatgcgccgc agatattttt cccgcgccgc aaaaatcccc agataagggc cgccaaaagc
     1261 cagcgggttg ccgaagctct ggccttcgcc cacaacgaaa tccgctccgc attcgcccgc
     1321 gggcttgaga aggcccaggc tggtggcgtc ggcaaatccg gcaatgagca tcgcgccctc
     1381 atcgtgggcc gtgtcggcca gcggctggag atcttcgatg cagccgaaaa aattcgggct
     1441 ttgcacgatg accgccgccg tttccttgtc cagcgcggcc gcaagcgcag ctttatccgc
     1501 ctggccggag ggcagccagg gaatttccgt aagcgtgcag ccgttggccc acgcgtaggt
     1561 ttgcaaaacc cgccggtact ggggatgaac cgaccgcgcc gcgatcagtt tcgatcgatt
     1621 ggatattttg gcgcacagca cggccgcttc cgccatggcc gaagccccgt catacatgga
     1681 ggcgttggcg atttccgtcc cggtgagatg cgcgatcatg gtctgatatt cgtaaatggc
     1741 ctgcagaatg ccctggctga tttccgcctg gtagggcgtg tacgccgtgt agaattccgc
     1801 gcgtccgacg atgtggttca ccaccgccgg aatgaaatgg tgataggcgc cggcgccggt
     1861 aagcgtcgcg cgcggcatcc ggtttttttc gctctgcgtc cgcatcagcg cccagatttc
     1921 ctgctcggaa agagacgccg gcatgccggg aatcttccgc aggcgaaatt tttgcggaat
     1981 atcggcaaac agttcttcga tgtttccaat gcctataccc gcctgcattt gcgcgatgtc
     2041 gtcgggggtg tgggggcagt aatccatgga tttagtgttt ctcctcccga atcagtgcgt
     2101 cgtaagcggt cgcgttcaga agagttttca actcttccgg atcggccact ttcaatcggg
     2161 cgatccaccc ctcgccgaaa gcgtcccggt tgacgagctc cggcgtgttt tcaagggctg
     2221 tgttgacgcc gatgacctcg ccgctcaccg gcgagtaaac gtcggacacg gatttgacgg
     2281 attccaccac cgccagcggc tctcccgccg cgactttttt gccgatcggg ggcagctcca
     2341 cgaacacgat gtcggtcagc atttcctggg cataatcggt gatgccgacc gaggccgtgc
     2401 cgtcgccccg gtccaggatc cattcatggt ctttcgaata ccatctgtcg gtgggatttg
     2461 gtttggacat gccaagtctc cttaagggat ttattttagt cttttgtaaa agggtagcgg
     2521 aacaattttt gccggactga cggcgtcgcg aatgccgatg gcgattgctt gttccggtga
     2581 ggcgcattcg gcgtcgataa acgccagacc gatcgccttg ttgagcgtcg gcgaaaatgt
     2641 gccggaggtg acaaagccga tggctctgcc gtctttcgtg accggatagc cgtgccgtgc
     2701 gatgcctcgt ccggtcatct caaagccggc cagctttttg cggacgccgg cctgcttcac
     2761 ggctgaaagc gcggtctttc cggcaaaatc cttgctccag tccaccagcc aggcgtaggg
     2821 aacctccagg ggacaggtct cctgcgtgat atcctggccg ttgagcatca tgccggattc
     2881 caggcgcagc gtgtcgcgcg cgcccagccc ggccggcttc atgcccagcc ccgctcccgc
     2941 gtccatcagg cggtcccata tcgcgccgat tttttcggcg gcggcgtaaa tttcaaagcc
     3001 gtcctcgccg gtgtagccgc tgcgggaaat gatcgccgga attccggcga cctgcgaggg
     3061 gataaaccaa taaaaacgaa tggtttttaa atcgtccggt gtcaaggtct gcaaaatcgc
     3121 ttccgagcga ggcccctgca aatccagttt gccggtcgag ttgctcacgt cggagagaga
     3181 tgcgtcgaac tttttttctt ccagaaggga ccgaatccgc tgaaaatccg gttcgcgcgt
     3241 tgtggcgttg gtcaccagca tataaaattc ccgatccatc ttgtacacgg tcagatcatc
     3301 gatgatgccg ccccggtcat tgagcagtgc ggagagtttc acctgtccgg ttttttgccc
     3361 cgccaggtcg cgcgtcaggg caaactgcag cagatccagc gcctgcggcc ctttcacttc
     3421 aatttccccc atgtggcaga tatcaaaaag agcggcgttg gcgcgggtcg cgcggtgttc
     3481 ctcgatgacg cttgtgtatt gcaccggcat ggcccagccg ccgaagtcga tgattttggc
     3541 gttcagagcg gcatgacggt cgtacaaagg cgtttttttc atgaaaagcg ctcctcgctg
     3601 agtctgctgc tatttgaaaa gcttgtcgat cagggcctgt ttgcttttcg ggctgatttt
     3661 ttcgcctttt tcgtttaagg cgcgaacctt gatttcggga aaccgctcca cgtcgatctg
     3721 aatgtccggg caggaaaggc atccttcggc cgccgccgcc ttctcgccgc gcgcctgggt
     3781 gattcgagga ttgaccagaa cgtcgtaatc ctccggcgac atttttttct tttggtcgtt
     3841 cccagtttta ttgcggaaaa tgatgattcg tttattcacg ccgatctgag gaccggccag
     3901 ccccgaagca tcgtcccggc aggtaaagga atcgacgagc gccttgattt cggctttttc
     3961 cgcggcgtca agcggcgttt tcatctccac cgactttttt cgcagcacgg aagtgtccgt
     4021 ttcgttgaca ccgttgtcat tccatagggt tagaactctt ttgggggcca tcttgtttgc
     4081 accggttcgc tgatttttca agacctataa caccgatcag tcgttatttc aagcaaacgc
     4141 agcttgcaaa ttggccccat cttttgttaa acaagcacaa cgtgtaccgg aatacagcga
     4201 aagaccttgc attgtgatca attccatcgt cgccagtacg gcgaaaaaac agtttgcccg
     4261 cgccctgaat tttcaagaag acgatacgac ggggctgccc gcctgcgccg acaaccaacg
     4321 gcgtctgctg tatgtccata ttcctttttg cgaagaactt tgcccctact gctcctttca
     4381 ccgcgttcgc ctcgacgaac cgctggcccg gcgctatcat gcggccctgc gcagggaaat
     4441 ggccctttac cgccgtcagg gctaccggtt ttccggcctg tacgtgggcg gcggaacccc
     4501 cacgattctg atggacgaac tggcggaaac gcttcagatt gcccgggatc tgtttcccat
     4561 agaatccgtt tcggtggaaa ccaatccgaa ccacctgacg gacgcccatc tttccgttct
     4621 gaaacgggcg ggagttcatc ggctttccgt gggcgtgcaa acgttcgacg acggtctgct
     4681 gaagaaaatg gcgcgctatg acaaatatgg ttcgggagaa gacatcgcgg cgcgtctcag
     4741 cgccgcgcag ggggtatttg acacgctcaa cgccgacatg atcttcaact ttcccgaaca
     4801 gaccggccaa atgctggagg ccgatctggc ggttttgctg aaactgagaa tgcagcagat
     4861 cacgttttac cctttgatgg tttccgagtt tgccagccgg cggatgcaaa aaacgctggg
     4921 cgacgtgaat ttccggcggg aaccgtcgtt tttccggctg atccggcggc gcctggagcc
     4981 ggattatgat tcatcctcgg cctggtgctt ttcgcgcaag aaagccgccc agtcgcttat
     5041 cgacgagtac gttgttgact ttgacgagta cgcgggcctg ggaagcggcg ccatcggcta
     5101 cctgcagggc atgtgctacg ccaacacatt cgacatcgaa aagtacattc aggatctgtc
     5161 gaaggatcgc ctgccgattc aggcctcgcg ccggttcgat ccgcccgacc ggatgcgcta
     5221 cgacttcctg atgaagcttt tcagcacccg gatgaatgtt gaggagatgg agaaaaaata
     5281 cggcggacgg tttttaaaaa ccctgtggaa ggaaatggcc gcgtttaccc tggccggatc
     5341 gtttcgctac tttacgcccc gtttgcatct gacgccccgc ggacgctatc tctgggtggt
     5401 catgatgcgg gagtttttca tcgcggtgaa caatttccgc gaccactgcc gccgggaggc
     5461 cggattgggc tagtgctgct gatgtatttt agtggtttgt catttcgacc gaagggagaa
     5521 atcttaagat tcctcgcata cgatagggac atggatttct cgatcgcttt gctccctcga
     5581 aatgacattt tatagggtaa gcagatgatg gcgcgttgga atctggagtg agttatggac
     5641 gacaaacgaa aagcattgct caaaggcctt ccaaaaattg acgacgtgat cgggcttttg
     5701 gaaaaaaacg gaatttattc aaaggcgtca aaggacattg tccgggaagc ctgccgggag
     5761 atcgtgcagc gcctgcgcga tcagatcctg tccgcggctg acaaagccct gccgtcactt
     5821 tcctttgacg cggcctcggc cgcccgcgcc gtggaggagc ggatggcgga tctgcatcgc
     5881 ttcaaattac ggcgtctggt caacgccacc ggcgtcattc tgcacaccaa tctggggcgc
     5941 gcgccactct gtgccgaagc ggtggaccgc atcgtggagg tcgggcgcgg ctactccaat
     6001 ctggaatttg atttgattcg cggcgagcgc ggcctgcgct acgatcatgt caccggcctg
     6061 atctgcgcgc tcaccggggc ggaggacgcc ctgatcgtca acaacaacgc ggcggctgtt
     6121 ctgctcacgc tcaacacgct ggccgagcac aaggaagcga ttgtctcgcg cggggagctc
     6181 atcgaaatcg gcggcgaatt ccgcattccg gatgtcatga ccaaaagcaa ttccattctg
     6241 cgcgaggtgg ggaccaccaa ccgcacgcgc ctggctgatt atgaaaaagc catcggtccg
     6301 aataccggcg tgattttgaa ggttcacacc agcaattacc ggattgtggg atttaccgag
     6361 gaggcggaac ttctgcccct ggtggcgctg ggcaagcacc acgggattcc cgtttttgat
     6421 gacctcggca gcggctgcct ggtggatctc gccgagttca ggctgcagca cgaaccgacc
     6481 gtgcgcgaag tgctggattc gggcgtcgat gtggtgacct tcagcggcga caaacttctg
     6541 ggcggtccgc aggcgggcat cattgtcggc aaaaaagaca tcgtggcgcg catcaagaaa
     6601 aatccgctga accgcgcgct gcgcatcgac aaattcacgc tggccgctct cgaggcgacc
     6661 ctgatgcgct atctggcgcc cggggacgcc ccccggaacc tgcgctccct tcgggcgctg
     6721 accgagccgc tgggcgacgt caaaaagcgc gcccgcaaac tgatggccaa actcaaacgc
     6781 gccggccttt ccggtttaac gtatgagctt aaagattcca tggcggccgc gggcggcgga
     6841 tcgcttccga ccgaggacat tccctcggcc gtcgtcacgg tgaagtccgc caagatgtcc
     6901 gccagccgaa tggaagccag tctgcgcagg gcggcggtgc ccgtcattgt ccgagtggat
     6961 gaagacgaaa ttcaaatcga tctgcgcacc gtggccgaag acgaattcgg ctttgtggtg
     7021 gacgccctgc gaggccttgc cgactccccg tgacgcaggc cggcccctgc cgcgcagtcg
     7081 ggaatccgag ggccgaggcc gcttccgaag acacatccac caagtcattt ccgcacaggc
     7141 ggaaatccgg gatatcagga gttgacgggc tgttgccctg gcgaaaagga tatttgggca
     7201 acagcccgat gagttgccgc ctgcgtcttc atgacgcctg attcgtcaag tttgcttttt
     7261 gctggagaat gtcgtgtcca tcgaaaacca ggaatcccaa aacctgtctt ttaccgtcga
     7321 ttccgcgcag gccgggcagc gcctggatgt atttctctcc caggccgatc cggctttctc
     7381 ccgatcgcaa attaaatacg ctgttgaaga aggcaatgta accgtgaacg gaagggagcc
     7441 gaaggcgagc cagcatctta aggcaggcga ccgggtggag ctgcatctgg agccggcggt
     7501 cgaggccgcc gccgccccgc aggacattcc cctgaacatt gtgtacgaag acggctccat
     7561 cattgtcgtc aacaaaccgg cgggcatggt ggtgcacccc gcgccgggca atccggatca
     7621 aacccttgtc aacgcgcttc tttttcactg ccgggatctt tccggcatcg gcggcttttt
     7681 gcggccggga attgtccacc ggctggacaa ggaaacatcc ggcctgatcg ttgccgccaa
     7741 gtccgacgag gcgcaccgca agctctccgc gcagtttgag aagcatgacg tgcacaagaa
     7801 atatatcgcg ctttgctggg gagacgtcaa aggcgcaagc ggcgagattg ttctgccggt
     7861 gggacgccat ccgaaagacc gcaaaaaaat gtccacgaaa agccggcatg gcaaagacgc
     7921 gctgacgctg tggaaagttc gcgagcgcta cggcacggcc acgcttctgg atattgaaat
     7981 caaaaccggc cggacgcatc agattcgcgt gcatctggcc gaacggggct atcccgtgat
     8041 cggtgatatg gtttacggcg gggcgtccaa agggcaaagc gtcaaaaacg cggcgctcaa
     8101 agccggcatt aaagcgctgt cgcgccaggc gctgcatgcc gcggaacttt cctttctgca
     8161 cccgcaaagc ggtgaaagaa tggtttttac cgcgccactg ccgcgggatt tggagggcct
     8221 gctggatctg ttccgctcgg cttcgccggg agggcccgga caaacgggtt tacaaacctg
     8281 gaaggatgct ttacgataaa cggcaaaaag cggcaaaatt cctcccgcgc ctctggcaaa
     8341 aagaggagac aaaggacgga agcaatgttt tcatggtcga acgcaaaagg catcccctat
     8401 ctgcaatcct ccggtcttgc ggcctgtggt tttttggagc acgctttctg cgcacgaaag
     8461 ggaggcgttt ccgaggatga ctaccagagc ctcaatatga gctttcggga aggcgacgag
     8521 gaatttcgcg tgctgcaaaa ctggaacaag ctggctgctg cgttcggcat cccggtggag
     8581 aacttcctgg tgctcaacca ggtgcaccgg gacggcattc tggttatccc gtcacggggc
     8641 gagtatttta cctcgcgcga agacctgaat tatgacgcca tcgtgaccga ccgcgtccat
     8701 ctggccatct gcattaaaac ggcggactgc gtgccggtgt ttctggcgga tcccgagaat
     8761 caggtgatcg ctgccgtgca cgcgggatgg cgcgggaccg ccgccggcat cacggccaaa
     8821 gtcgttcgcc tgatgcaaaa tcaatacggc tcccggccgg tcgatctgct ggcggcgatc
     8881 ggcccgtcca tcggccgctg ctgctatgaa gtggacgcgc ccgccgctga cgcgtttcgg
     8941 cttcatccgg acgcaaaaaa ctttttgttt ccgtcaaagc gcaaggatcg ctggatgctg
     9001 gatctggttc aagccaatcg cggacagctc atagactgcg gcattcccga cgcctccatt
     9061 gacgcatccg gtttgtgcac cgtctgccgt gaggacgatt ttttttcaca ccgcggctcg
     9121 ggcggcgtca ccgggcgaca aatcaatttt ctcatgctga agggagaccc gatctgcaaa
     9181 gccctgttgc tgaacgacga tttgcgtcat atccattgat aaaaaccgta aacaaaaggg
     9241 aggtctcatg aatctcaagc ctttgtttga gcccaaaacc atggcggtca tcggcatttc
     9301 cgcaagcaac gaccgccatc ccgccaatgt gatctatgac aaaaacaacc atcgctatcc
     9361 cgtaaaggtt tacgcggtca atcccagggg cggctcgctg cataacgaaa cggtttatcc
     9421 ccgcgtggcg gagattccgg ccaaaatcga tctggccgtc attgcgacgc gggcggagct
     9481 ggtcccggac gttatggccg actgcattgc ggcgggggtg aaatccgcca ccgtgatttc
     9541 cggagggttc agcgaaggag gacggggcga tctgcaggat cgcatcgtgg acatggccag
     9601 ggaggccggg ctgcctttta tcggccccaa ctgcctgggc gtatatgttc ccttccgcgt
     9661 tgatacgttt ttcctgccga tcgaacgcat gatcaagccg gaggcgggcg gggtgacgtt
     9721 cgtcagccag agcggc
//
LOCUS       DAOSIM010000053         9614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_6849_length_9614_cov_6.919971, whole genome shotgun sequence.
ACCESSION   DAOSIM010000053 DAOSIM010000000
VERSION     DAOSIM010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9614
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6849_length_9614_cov_6.919971"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..727)
                     /locus_tag="PLR47_03815"
     CDS             complement(215..727)
                     /locus_tag="PLR47_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12097.1"
                     /translation="MKTKPISLPRILLAAVLAASISFTPAAAQEFRTINAIPTPGIIP
                     PGAAPVEEFRPIAKSAAADSVKKLMGAWNGGGIQQYLGDDFYDKSGLADAISTKVPKD
                     AAIRIMSVQGVQTLQQFQRNKDGGGHEMISEVSVTADTQIEYNDPTKGFVKFGGVNEY
                     ILRITEEAAQ"
     gene            complement(741..1595)
                     /locus_tag="PLR47_03820"
     CDS             complement(741..1595)
                     /locus_tag="PLR47_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12098.1"
                     /translation="MILNCLFLFVSFQNVLAEEIILGKDSRPLFPAELTERFVGALPS
                     SDKSSQAGKAQDALAESGAVLLGRGKTKITKIEVVRVIPVRGNNPLNQIEPVNNATIN
                     GRLYFASVEELQKMMQGGGDSPAPGPGPSPPPPPPPPPPTPFAGCIGQSAPFKSTSGF
                     QTCLGKQMPTFSAGLFVDFAASACKVVGGDSGWNFLAFDIMVPLLSPDCTYTDVAAFP
                     DFAYAEFIPPTGSGFVRIPLKFDVEGRFYLQSKKAPYAGRKLVVFATVKKISNYKFEL
                     FISNVSPP"
     gene            complement(1617..4493)
                     /locus_tag="PLR47_03825"
     CDS             complement(1617..4493)
                     /locus_tag="PLR47_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12099.1"
                     /translation="MFRGKFYFVILPALVLWTFLLLQGFGNQVLAKDPEKGKKAEQLT
                     ISQPASFIAENLHNTIESLRNLEQTYEKNCGSLLLESISRGGNTQEIAGKLPKPKTLR
                     FNVAYPEEKLPQLSADALSDLRNPRYTPVLGGISYFEKAYPGYVAADSETAALLNLKN
                     RIPPTDIGFSPKIGFSILQDKFSLVSNFCGVMRRAVSGGEANVFSVPADVRGKLGVLV
                     SAAGGATVLDSGTVAFSSPGLPAVTISGKGGLQGINPELCQGRNEWTDYVNAGRDFTR
                     TIQFQSIKFKDFGIPGLDFNLPGAKAPFLDYFIPVNQSYNGVIASTKSLSTSAPERFM
                     LDIWADPYAIGRISPILVIDTGQEKEKPFEISDFPVTVHTDVKSPGPILPDGKGFLQY
                     AGGTGASVIAWNLGKLETEKRMVQHRFTVNSAAVEPAPEMRDGELAQGGEYSVKLMVQ
                     GEADMGGDYTVNWSSAGPAKWKESRTYFKKEGNVWSAVNTLIVDVLDKWPEGVGKKVD
                     FSASASYLPIIGSNPQKKDLLRMLQILSPPPRQDVFSFRGSAKLAMPVAEKLKLLVRY
                     GNRTPREMDEIDLFYPNHLAADVRLMPFLHLRNVKDPLALGDVKVPVQIEFKSTNPGA
                     AAVTTGGRLTSQSVYPMETEISAKISGYANISDPKENYKMLETAQAKLISNTIWVNVN
                     RLILSSTLQNGKTLYKLSASGAGDMSKYKALWKTAGIAGASETATQFTKDGDAFVATL
                     LSGSRVDHVRIVLDDLALAQLDAKSTLAQANIVLLPTRPPAAAVGKDTGDNSNDFKSM
                     TDCVSTVARWADMWGFETGGKPETYCRQKLQQQTQEAQNKRAQNRFAEQLKKRGLDLV
                     VFNDAMKVGAAVTGLSREFWERTWCRWRISSESKLYLEQDMTPVRMSSEMLGGCFNVV
                     KGIQEYFDPAAMIHVELSLDTDMPNYPLFVDPEKYVGSADEFRR"
     gene            complement(4513..7257)
                     /locus_tag="PLR47_03830"
     CDS             complement(4513..7257)
                     /locus_tag="PLR47_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12100.1"
                     /translation="MKNKFAGLMILFLVFFSFFAGISPAANQEGEKTDCANLKIQIND
                     IDKKIKRSCQDVQSLMDGLIDHWQSYYSSENQRLSIYELYNQKQWGILKDFVVSPDAN
                     AKAEPGQFYNLFVSLKNEARETGLLIDGTPPWSDKQRRAGIQISNSEFAFAPDAKSYF
                     YFGNPEKNEAGKAELSKKLNDIKSKWNEHKKKYDEFIKSDLDLANKDLASLSEKMAKA
                     KAGYFNNADQLSKAAYSGDYEHCLGKMAPASSSYTYIDGTEKLFIKGKKDTVEGLPGM
                     GLQFLQPTNLPVLARTNIPDCPFQLHLKRDGDKARAFVEEILKLELEKQERDTIELGI
                     DFVDETLSFAFDAAFHIVEATAPLGDAMIDIASHPIDSTVNGAKAVIEFGDKVLDRGY
                     LYKIKAEGGIGEAIGTALESSADKLGNLGSQLALRAVDSDRFTANPGDPPQKKLEKML
                     EQIESARQVNVGLKEMAKVTGEIAMLWKADAVIAKTGQAVSLAKAGVAGGKFESLANQ
                     LKALKAQNPALTAQVEAKTKSLEKIIEGQKDVNGKLEILQKKVVNEPVVKPFAGQPTK
                     AKATVQVSTAEGQNVSLTLGEQLGKGSTSTAFIDATNDSRIIRQTVLDKKAVAAAKLD
                     DFGRQALEKDVKSDSLRVAKREAVYTTPGDELGIEKAKKIEVVERVAEDAATTIKKQG
                     GILTEGQKMAYEQGLRDINSKGFAWVDNHSGNFAFEKLPGEDRWRVVVFDTGNIVKVT
                     GNTAEELAKNARAMQIRVNELGKTMEGKTFLEQAEIREKILGAYEKPLVDIKAMGIES
                     SDSIGMAAGDIRINTGNIAKMTDQELAVSLQGKTADAVLAAPAEDAIKARKKASPVPE
                     AAEIAESLEQAAGYTLMTNAASRLSDCAECVKYRKLLIAGEDPEKLKKMGNPCANLNH
                     "
     gene            complement(7322..8074)
                     /locus_tag="PLR47_03835"
     CDS             complement(7322..8074)
                     /locus_tag="PLR47_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12101.1"
                     /translation="MKINKVAEVMAGVLILASFAFPHAAFCENLSPDIIKITAPKESA
                     SGSYPHRSFRQFAVEGWACGDNLAPQIIHDIRTANLISEELVDKNQLLREGIQSLGLL
                     MEESRQQGLNSNQLSENASRLSESILSRTAAVDLTLTDYQAHRTISARSMEEIDRYYI
                     QKFHKEIEDFQKAAANGVNRLADTSAKFVGYETTLEQVMDNDETYVIVNCYTKSDLDV
                     FVSNQTQAYQTAIALFDEYQKLRRDVEKYRKR"
     gene            complement(8231..>9614)
                     /locus_tag="PLR47_03840"
     CDS             complement(8231..>9614)
                     /locus_tag="PLR47_03840"
                     /inference="COORDINATES: protein motif:HMM:NF020071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HOG12102.1"
                     /translation="PPPSAWRAAKELLKELGALDANGSITPVGRAMARLPLHPRLGRL
                     LLRAADLDCPSFGADLAALLSERDIIRHSRSSFPHSAGGFDLSDRLGLLGQWRQNKML
                     PKTADAFALRAVDRTSQQLRRLMGKTSEDAGVENGGMIPRLLLFAFPDRIAKRREDDN
                     SRYVLRQGRGVRLSAKIAPGGSPCLVAVVVDKGEKAEGMVHMAEPLTEELIREALQDQ
                     IETVRQVVWDKQEGRIIATREERLGTIILSAKPVNPPKEEVASVLCEAIRSRTVMIAF
                     SPEVLQFQARVRLMQQTFPEEKWPDISSETLISAPQDWLLPRLSGIRNAQQLADLNML
                     PALTERLTWEERRLLAERAPVTIAAPSGHNIPIDYVSGDIPVLAVKLQEMFGLADTPV
                     IAGGRIKLLLQLLSPARRPVSITRDLKQFWNTGYPQVKKELKGRYPRHPWPDDPWNAV
                     PTRKTKARNG"
BASE COUNT         2060 a         2602 c         2479 g         2473 t
ORIGIN      
        1 cgccttccgt ttgggcggtc acgtcggccg gcgcaaggca gataaacatc atcagaaaaa
       61 cacaagccag ccgccggaca gagccggtta ttccgccgcc gcgacgcggg atgctttgaa
      121 gtctcacgga atctctccta ttgcgtccga ccaggacgaa accctgcgcg atagagcgcg
      181 aaattcgggg aagagcgcca aatgcccgcg gcggttattg cgccgcctcc tccgtaattc
      241 tgagaatata ttcattgacc ccgccgaatt taacaaaccc ttttgtcgga tcattatatt
      301 caatctgcgt gtccgccgtc acggaaacct cgctgatcat ttcatggccg cctccatctt
      361 tgtttctctg aaactgctga agcgtctgaa cgccctgcac ggacatgatt cgaatggctg
      421 cgtctttcgg aaccttggtg ctgattgcgt cggcaagccc gctcttgtcg taaaaatcgt
      481 cgcccaggta ttgttgaatc ccgccgccgt tccaggcgcc catcagcttc ttcacggaat
      541 cggcggccgc gcttttggcg atcggacgaa actcctcaac cggggcggcg cccggaggaa
      601 ttattccggg tgtcggaatg gcgttgatgg tccggaattc ctgcgcggcg gccggtgtaa
      661 agcttatgct tgcggcgagg acggcagcca gcagaatccg cggaaggctg atcggttttg
      721 ttttcatgct ttgtttcctc ttacggcggt gaaacattgg aaatgaacag ctcgaattta
      781 taattgctga tttttttcac cgtggcgaaa acaaccagct tccgccccgc gtagggtgct
      841 tttttggact gcaaataaaa gcggccttcc acatcgaatt tcagggggat cctcacaaat
      901 ccggaaccgg tgggcggaat gaattccgca taggcaaaat caggaaaggc ggccacgtcc
      961 gtgtaggtgc agtccggaga cagaagcggc accatgatgt caaaggccag gaagttccag
     1021 ccgctgtctc caccgacgac tttgcaggcg cttgcggcga aatccacaaa aagtcccgcc
     1081 gaaaaagtcg gcatttgctt gcccaggcag gtctggaaac cggaggttga tttgaacggc
     1141 gccgactgtc cgatacaacc ggcaaagggt gtcggaggcg gaggcggagg cggagggggc
     1201 ggggaggggc ctggaccagg cgctggactg tcgccgccac cctgcatcat tttttgcagc
     1261 tcttcaacgg acgcaaaata cagccggccg ttaatcgtgg cattgttgac cggttcgatc
     1321 tgattgagcg gattgttgcc gcgcaccgga ataacccgta caacctcgat tttggtaatt
     1381 ttcgtttttc cccggccaag taaaaccgcg ccggattccg ccagcgcatc ctgcgctttg
     1441 cctgcctgcg aacttttatc ggaagacggc aatgcgccga caaagcgctc cgtcagctcg
     1501 gccggaaaaa gcggccggct gtcttttccc aaaatgatct cttccgccag aacattttga
     1561 aaagagacaa ataaaaacaa acaatttaaa atcatcaaca tccgttgaaa cattttttac
     1621 ctccgaaatt catccgcgct gcccacgtac ttttccgggt caacgaacag cgggtagttc
     1681 ggcatatccg tatccagaga cagctccaca tgaatcatcg cggcgggatc aaaatattcc
     1741 tggattccct tgaccacgtt aaaacagccg cccagcattt ccgaggacat ccggacgggc
     1801 gtcatgtctt gttccagata aagtttgctc tccgaggaga tgcgccaccg gcaccatgtt
     1861 ctctcccaga attcccggga aagtccggta acggccgcgc caactttcat ggcgtcgtta
     1921 aacaccacca gatccagacc ccttttcttc agctgttcgg caaaacggtt ttgcgcccgt
     1981 ttattctgtg cttcctgcgt ctgctgctga agcttttgcc ggcagtaggt ttccggtttt
     2041 ccgcccgttt caaagcccca catatccgcc cagcgggcaa ccgtgctcac gcagtcggtc
     2101 atgctcttga aatcattgga attgtcgccc gtatctttgc cgacggccgc agcaggcgga
     2161 cgagtgggca ataatacgat attggcctgg gccagcgtgc ttttcgcgtc cagctgggcc
     2221 agcgccaaat catccagaac aatccggaca tgatcaactc tgctgccgga caataatgtg
     2281 gccacgaaag cgtcgccgtc tttggtaaat tgcgtggccg tttccgacgc cccggcaatt
     2341 ccggctgtct tccataaggc tttgtacttg ctcatgtctc ctgcgccgct cgcggagagc
     2401 ttgtagagcg ttttcccgtt ttgcaatgtg gaagataaga tcaggcggtt gacattgacc
     2461 cagatggtgt tggagatcag cttggcctga gccgtttcca gcattttgta attttctttt
     2521 ggatcgctga tgttggcata gccgctgatt ttcgcgctga tttcggtttc cataggatat
     2581 acggactggc ttgtcaaacg cccgcccgtc gttacggcgg cggcgccggg attggtggat
     2641 ttaaattcaa tctgcacggg aactttcaca tcgccaagcg ccagcggatc tttgacgttc
     2701 cgaagatgca aaaacggcat caggcgaaca tcggcggcca gatgattggg gtaaaacagg
     2761 tcaatttcat ccatttcccg gggtgtccgg ttaccgtaac gaaccagaag cttcagcttt
     2821 tcggccacgg gcatcgccag ctttgccgat ccccggaagg agaaaacatc ctgccgcggc
     2881 ggaggagaca aaatctgcag catcctcaac agatccttct tttgcggatt gcttccgatg
     2941 atcggcagat aacttgcact ggcggaaaaa tcgacttttt tgcccactcc ctccggccat
     3001 ttgtccaaca catccacaat cagtgtattg acggccgacc agacattgcc ttctttttta
     3061 aagtaggtgc gggattcttt ccattttgcc ggtccggcgg aactccagtt aactgtatag
     3121 tctccaccca tgtcggcttc tccctgaacc atcagtttta cggaatattc tccgccctgt
     3181 gccagttctc catcacgcat ttcgggcgca ggctcaacgg ctgcggaatt gaccgtgaaa
     3241 cggtgttgaa ccatccgctt ttctgtttcc agcttgccca gattccaggc aatgacactt
     3301 gcgccagtgc cgcccgcata ctggagaaat ccttttccgt caggcagaat cgggcctggt
     3361 gacttgacgt ccgtatgcac ggtgacggga aagtcggata tttcaaatgg tttttccttc
     3421 tcctgacctg tgtcgataac taaaatggga ctgattcggc caatcgcata aggatcggcc
     3481 caaatatcga gcatgaaccg ttcaggcgca gaggtggaaa ggcttttagt gctggcaatg
     3541 acgccgttgt aactttggtt gacgggaata aaatagtcca ggaaaggcgc tttggccccc
     3601 ggaagattaa agtcgaggcc cggaatgccg aaatccttga atttgatgga ctgaaattgg
     3661 atggttcttg taaaatcccg gcctgcattg acatagtccg tccattcatt tcggccctgg
     3721 caaagctcgg ggttgattcc ctgcaatccg ccctttccgg aaatcgtcac tgccggcagg
     3781 ccgggcgaag aaaatgcaac cgttccggaa tccagaaccg ttgcgccgcc cgccgcgctc
     3841 accagcacgc ccagcttgcc ccgcacatcg gcaggcacgc taaagacatt ggcttcgccg
     3901 ccggatacag cacgccgcat gacgccgcaa aaattactga ccagagaaaa tttatcctgc
     3961 aagatggaaa aaccgatttt gggcgaaaaa ccaatgtcgg tgggaggaat cctgttttta
     4021 agattgagga gcgcggctgt ttctgagtct gccgccacat agcccggata ggctttctca
     4081 aaataggaga tccctcccag aaccggtgtg tatctagggt tgcgcaaatc gctgagagcg
     4141 tccgcgctca gttgcggcag cttttcttcc gggtaggcaa cgttgaaacg cagcgttttg
     4201 ggttttggca gcttgcctgc aatttcctgc gtattgccac ccctggagat ggactccaaa
     4261 agcagggagc cgcagttctt ttcataagtc tgctctaaat ttcttaatga ttctatggta
     4321 ttatgaaggt tttccgcgat aaacgaggcg ggctgggaaa tcgtcaactg ctcagccttt
     4381 tttccctttt ccggatcttt ggccaaaacc tggtttccga agccttgcag cagcaaaaag
     4441 gtccaaagca caagcgcggg caggatcacg aaataaaatt tacctctgaa catgctttgt
     4501 ctcctcatcg acctaatgat tcagattggc gcagggattg cccattttct taagcttttc
     4561 cggatcttcg ccggcaatca gcagctttct gtatttcacg cattccgcac agtcggacag
     4621 tctggaggct gcattggtca tcaaagtgta tcctgccgcc tgttccagtg actcagctat
     4681 ttccgcggct tccggaaccg gggaagcctt cttccttgct tttattgcgt cctcggccgg
     4741 agcggctaaa acggcatcag ccgttttccc ttgcagggac actgccagtt cctgatcagt
     4801 cattttagca atgtttcccg tgttgatgcg aatgtctccc gccgccatgc cgatgctgtc
     4861 cgagctttca atgcccatgg cttttatatc gaccagcggc ttttcataag cgccgagaat
     4921 cttttcccgt atttccgcct gctccaaaaa agtttttcct tccatggttt tgcccagttc
     4981 gttgacccga atctgcatgg cccgggcgtt tttggccagc tcttccgcag tgtttcctgt
     5041 aactttcacg atgttgcccg tatcaaacac caccacgcgc cagcgatctt cacccggcag
     5101 tttttcaaag gcaaaattgc cgctgtgatt gtccacccag gcaaatccct tgctgttgat
     5161 gtcgcgcaaa ccctgttcat aggccatttt ttgcccttcg gttaaaattc ctccctgttt
     5221 ttttattgtt gtcgccgcgt cttccgccac tctttccact acttcaattt tttttgcttt
     5281 ttcgatgccc aattcgtcgc ccggtgtggt gtaaacagct tctcgtttag ctacgcgaag
     5341 cgagtcgctt ttaacgtctt tttccagagc ctgtcggcca aaatcatcaa gtttggcggc
     5401 agcaacggct tttttgtcca gcacggtttg tctgataatc cgcgaatcat tggtcgcgtc
     5461 gataaacgcc gtgctggtgg aaccttttcc cagttgctca cccagagtta gagaaacatt
     5521 ttgtccctcc gctgttgaga cctgaaccgt cgctttggcc tttgtcggct ggccggcaaa
     5581 aggtttaacc accggttcat tgacaacttt cttttgcaat atttccagct taccgttgac
     5641 gtctttttgt ccttcaataa ttttttccag acttttggtt tttgcttcca cttgcgcggt
     5701 cagggcggga ttttgcgctt tcagggcttt gagctgattg gccagagact cgaatttgcc
     5761 gcccgcaaca ccggctttgg ccagcgacac cgcttgcccc gttttggcga taaccgcgtc
     5821 cgccttccag agcatggcga tttcgccggt aactttggcc atttccttca ggccaacatt
     5881 tacctgccgg gccgattcaa tttgctccag catcttttcc agtttttttt ggggcgggtc
     5941 acccggattg gcggtaaaac gatcagaatc aacggcgcgg agcgccaact gtgaaccgag
     6001 gttgcccagt ttatcggcgg atgattccag ggctgtgcca atggcttctc caatgccgcc
     6061 ttccgccttg attttgtaca ggtagccgcg gtcgagaact ttatcgccga attcgatcac
     6121 cgccttggcg ccgttgacag tgctgtcgat gggatgagaa gcgatatcga tcatcgcgtc
     6181 gcccaggggc gcggttgcct ccacaatgtg aaaggcggcg tcaaatgcga aagacaaagt
     6241 ttcatcgaca aagtcaatgc ccagttcaat cgtgtctctt tcctgttttt ccagctccag
     6301 cttgaggatt tcttccacga acgcccgcgc tttgtctccg tcgcgcttca gatgaagctg
     6361 gaacggacaa tcgggaatat tggtcctggc cagcacgggc agattggtcg gttgcagaaa
     6421 ctgaagaccc attcccggca ggccttccac ggtgtccttc tttcccttga taaacagctt
     6481 ttccgttcca tcaatatatg tgtaggagga ggaggcgggc gccattttcc ccaggcagtg
     6541 ttcgtagtcg ccgctgtagg cggctttgct gagctgatct gcattgttga aatagccggc
     6601 tttggctttc gccattttct cactcagaga tgccagatct ttattggcca aatccagatc
     6661 gcttttgata aactcgtcgt atttcttttt gtgctcattc catttgctct tgatgtcgtt
     6721 cagttttttg ctgagttcgg ctttgccggc ctcatttttt tccggattgc cgaaatagaa
     6781 ataggatttc gcatcggggg caaaggcaaa ttccgagttg ctgatctgga tgccggccct
     6841 tctttgcttg tcggaccagg gcggcgtgcc gtcgatcaaa agtccagtct cccgcgcttc
     6901 gttttttaat gaaacaaaaa ggttataaaa ctgacccggt tctgctttgg cattggcgtc
     6961 gggcgagacc acgaaatctt tcaggatgcc ccactgtttt tgattgtaaa gttcatagat
     7021 ggaaagccgt tggttttcac tgctgtaata ggactgccag tgatcgatca ggccatccat
     7081 caaagattgc acgtcctggc aggacctttt gattttcttg tcaatatcgt tgatctggat
     7141 tttgaggttt gcgcagtccg ttttttctcc ttcctgattt gcggccggag aaatccccgc
     7201 aaaaaaggaa aagaaaacca aaaacaggat catcaatccg gcaaatttgt ttttcatgct
     7261 tcgcactttc gctttttcgg gaaaaaacta acgtttgata ttattcacaa tgagatgctg
     7321 cttaacgctt ccggtatttt tccacgtcgc gtctgagctt ttgatactca tcaaaaagcg
     7381 cgatcgctgt ctgataagcc tgcgtctggt ttgagacaaa cacatccaga tcggattttg
     7441 tataacagtt gacgatgaca taggtttcat cgttgtccat cacctgttcc agggttgttt
     7501 cataacccac gaacttcgcg gaggtatcgg ccaggcggtt gacgccattg gctgctgctt
     7561 tctgaaagtc ttcaatctcc ttatgaaatt tttgaatgta gtaccggtcg atttcttcca
     7621 tgctgcgggc ggaaatcgtt ctgtgtgcct gataatcggt cagggtcaga tcaacggcgg
     7681 ccgtccggga caaaattgat tcgctgaggc gggacgcgtt ttccgaaagc tgattggaat
     7741 tcagcccctg ctgcctgctt tcctccataa gcaaacctag ggattgaatg ccttcccgca
     7801 gaagttggtt tttgtcgacc agttcttcgg aaatcaggtt ggcggtgcgg atgtcgtgaa
     7861 tgatttgcgg agcaaggttg tcgccgcagg cccatccttc tacagcaaac tgccgaaagg
     7921 aacgatgggg ataagaaccg ctggccgatt ctttcggcgc ggtgattttt atgatgtctg
     7981 gcgaaaggtt ttcacaaaag gcggcatgcg gaaaggcaaa agaagctaaa atcaggactc
     8041 ctgccatgac ttccgcaact ttatttattt tcatgtttac tccgcccagg aaacatgatt
     8101 tttgatttgc attatagtct tgccatctgc gcaattcaag aaaatattaa atacagaatg
     8161 acttttttgg cctccgctca aaattaacat caaactcttg tcaagtccgg cctttttgac
     8221 ggatgcattg tcaaccgttc cgggcttttg tctttctggt cggaacggcg ttccaggggt
     8281 cgtcgggcca gggatgcctg gggtagcggc ccttcagctc ttttttcacc tgcggataac
     8341 cggtgttcca gaattgtttc agatcccggg ttatcgaaac gggccttcgg gcgggcgaaa
     8401 gaagttggag aagcaattta attcttccgc cggcaataac cggggtgtcg gcaagaccga
     8461 acatctcctg gagtttgacg gccaaaaccg ggatgtcccc ggaaacataa tcaatcggga
     8521 tgttatggcc gctgggggcg gcgatggtta cgggggcacg ctcagcaaga agacgccgct
     8581 cttcccacgt gagcctttcc gtaagcgcag gcagcatgtt caggtcggca agctgctggg
     8641 cgttgcgaat gccggaaagc cggggcaaaa gccagtcctg tggcgccgaa ataagcgttt
     8701 cggaagagat gtccggccat ttttcttcgg gaaaggtttg ttgcatcaag cgcacccggg
     8761 cctgaaactg caggacctcc gggctgaagg cgatcataac ggtccttgat cggatggcct
     8821 cgcaaaggac tgaagcgact tcctccttgg gtggatttac gggtttggcc gaaaggatga
     8881 tcgtccctaa acgttcttca cgggtggcga taattcgtcc ctcctgtttg tcccaaacga
     8941 cttgccgcac agtttcaatc tgatcttgca gtgcctcacg gatgagctct tccgtcagcg
     9001 gttcagccat atgcaccatg ccttccgcct tttcgccctt gtccaccaca acggcaacca
     9061 aacagggact cccgcccggg gcaatttttg ctgacagccg aacgcctctt ccctgccgca
     9121 aaacataacg gctgttgtcg tcttcgcgac gcttggcgat gcggtcggga aaggcaaaaa
     9181 gaagcagacg gggaatcatg ccgccgtttt caacgcctgc gtcttcagag gtcttgccca
     9241 tcaaacgccg gagctgctgc gatgtcctat ctaccgcacg caaggcaaaa gcgtcggccg
     9301 ttttcggcag catcttgttt tgccgccatt gcccgaggag accgagacga tcgcttagat
     9361 caaacccgcc ggctgaatgc ggaaaagagg accgggaatg acggatgatg tcccgctcgg
     9421 acagcagcgc cgccagatcc gctccaaagg acggacagtc caaatcagcc gctctcaaaa
     9481 gcaggcggcc gaggcgcgga tgaagaggca ggcgcgccat ggcccggcct acgggtgtaa
     9541 tcgaaccgtt tgcatccagg gcgccaagtt ctttgagcaa ttcctttgcc gcgcgccagg
     9601 ccgaaggcgg cggc
//
LOCUS       DAOSIM010000054         9605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_6857_length_9605_cov_7.989948, whole genome shotgun sequence.
ACCESSION   DAOSIM010000054 DAOSIM010000000
VERSION     DAOSIM010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9605
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6857_length_9605_cov_7.989948"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..2070
                     /gene="thrS"
                     /locus_tag="PLR47_03845"
     CDS             157..2070
                     /gene="thrS"
                     /locus_tag="PLR47_03845"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOG12103.1"
                     /translation="MDVKVIFPDQSEQTFPRGVTVSEAVGSWKKEALAAAVAADVNGV
                     SVDLSFALSGDAKVEPIDISSKKGISILRHSVSHVMAQAVQEVFQGAKVTIGPSIEDG
                     FYYDFEYAEPFTLDDLEKIEKKMKEIVAADHPFVREELPREKAAELFAAKGEDYKVEL
                     IRDLPADVSVVSLYKDGDYVDLCRGPHIPRTGMIKAFKLLSVAGAYWRGDERNKMLQR
                     IYGTGFADEQALQDYLTLLEEAKKRDHRKLGRELDLFQVNDEAGAGLVIFHPKGMLLR
                     YLIEEWERKEHLKRGYDMVMGPQILKVDLWKKSGHFDHYRDNMYFTEVDEQTYGIKPM
                     NCLSHMLIYKSKIRSYRDLPLRYFELGTVHRHEKTGVLHGLMRVRQFTQDDAHILCMP
                     EQLNAEIRAIADFVHYAMGIFGFEYEVELSTRPEHSIGSDADWDLATKALENALVDNK
                     IPYEVNEGDGAFYGPKIDFKLKDALKRKWQCATIQCDFTLPERFDLSYIGPDGEKHRP
                     VMLHRVILGAIERFMGVLIEHYAGAFPVWLAPTQCVVLTVTDKQIPYAESVRGRLVEA
                     GIRCEPYFDNDKLGYKIRQAQMQKVPYMLVIGDKEVAACAVAPRARDGRNLGAMPVDE
                     FIALIQEACGSKK"
     gene            2126..2617
                     /gene="infC"
                     /locus_tag="PLR47_03850"
     CDS             2126..2617
                     /gene="infC"
                     /locus_tag="PLR47_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOG12104.1"
                     /translation="MNREIKSANVRVINEEGQPLGVIPLEEAMAQAERAALDLVEVSA
                     NADPPVCKIMDYGKYRYKQSKKIHDARKSQTVIHVKEIRLRPKTEAHDLQVKIKHIKK
                     FLEQRDKVKISMIFRGREIAFTEIGRKIMEQIKAALADECIMDQEPRLEGRSMVMIVS
                     PKK"
     gene            2648..2845
                     /gene="rpmI"
                     /locus_tag="PLR47_03855"
     CDS             2648..2845
                     /gene="rpmI"
                     /locus_tag="PLR47_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOG12105.1"
                     /translation="MPKMKTHRGAAKRFSVTAKGKVKRSKAFASHILTKKTTKRKRTL
                     RKAALVHGANEKAIKRLIPYL"
     gene            2895..3254
                     /gene="rplT"
                     /locus_tag="PLR47_03860"
     CDS             2895..3254
                     /gene="rplT"
                     /locus_tag="PLR47_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOG12106.1"
                     /translation="MARIKRSVTARKKRRKILKMAKGFFGARSRLLRTATEAVNKAMS
                     YAYRDRRGRKREFRALWIARINAAARLNQISYSRLMDGLKKANIGLDRKVLAELAVND
                     PQDFARIVETAKGEQAA"
     gene            3251..4267
                     /gene="pheS"
                     /locus_tag="PLR47_03865"
     CDS             3251..4267
                     /gene="pheS"
                     /locus_tag="PLR47_03865"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOG12107.1"
                     /translation="MTGDLQLLEKDALAELEKAGGEDSILAVRTKYLGRKGLLTGMLR
                     NISSVPAEERRVFGMRCNEVKELLEKNIEEALRRKAEDQKAEWLAAQQIDVTLPGRGI
                     RAGRVHPVIQIRNEICGIFAAFGFSVVEGPEVELDYYNFEALNIPKDHPARDMQDTFY
                     IEDNMVLRTHTSPVQVRIMEKAKPPVRILSPGRVYRPDSDVSHTPMFHQIEGLLVDRG
                     VSFGDLKGILTAFLRKVFGEGTILRFRPSFFPFTEPSAEVDIRCVMCGGKGCRVCGQS
                     GWLEILGSGMVDPAVFQNVGYDPEVYTGFAFGMGLERIAMLKYGISDIRLLFENDIRF
                     LKQF"
     gene            4282..6690
                     /gene="pheT"
                     /locus_tag="PLR47_03870"
     CDS             4282..6690
                     /gene="pheT"
                     /locus_tag="PLR47_03870"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOG12108.1"
                     /translation="MLVSLKWLKDYIDVETAPEELADRLTMAGLEVDEIRTLSPRFSG
                     VVVAKILSVRPHPNADKLSLCDVTDGAQTYPIVCGAKNIHPGDIVALAKIGAIIPGGY
                     TIKSTTLRGEKSEGMLCSEAELEIGDDASGIVQLPAGMTPGTPLEEALDLNDTVLDVG
                     VTPNRSDCLSMIGMAREAAALTGKTLKMPEGKVREAAEDVSLLSSVTIEDPDLCPRYT
                     ARLIRNVTIGDSPVWMKTRLEAAGLRAINNVVDVTNFVMLEMGQPLHAFDFHFLEERR
                     IVVRKSKPGEEFVSLDGKSRILPENTLLICDGKKPVAIAGIMGGLNSEVKEDTRTIFL
                     ESAYFNPSSIRRSSRRLAMPTDAAFRFERGIDPEGVIRALDRAAQLIADLSGGEVCAN
                     RLDEYPAKIKPVENILLRMDRAGQVIGVAVPAREAVRILKSIGMTLKREGRGVYRVTP
                     PSFRVDIEREIDLIEEIARLYGYDRVPSTLPSVSVTELDAIPRLALEERIRLLLTGFG
                     YSEIINYSFTTPFCAEALGLPPEDERRRFVVIRNPLTEDQSVMRTTLTYGLLETLKKN
                     INNASWNLKLFEIGRTFLRRGDGELPEERNILAALAAGKASDDLWGLKAGVDFYDLKG
                     CLENIFQDLKFDSMRFRAEKPQSYLHPGQSCGVYADDVRIGSMGKAHPDVLKKMDIKS
                     DAYVFEIDLDLLGAKIRPRVRCREISKFPAVLRDVAFVLPSSMESEKMLEMVLRQRED
                     LLENVSIFDIYAGKGLEEGTKSLGLRFSYRALDRTLTDAEVSAIHDRIVKDTIRLTGA
                     KIRA"
     gene            6724..6999
                     /locus_tag="PLR47_03875"
     CDS             6724..6999
                     /locus_tag="PLR47_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HOG12109.1"
                     /translation="MTKIDIIQNVYEKLGFSKKESSDIVESVFDIIKDSLAAGERVKI
                     SGFGNFMVKEKRARRGRNPQTGEEISITARRVLTFKSSQVLRKSLNS"
     gene            7116..7454
                     /locus_tag="PLR47_03880"
     CDS             7116..7454
                     /locus_tag="PLR47_03880"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOG12110.1"
                     /translation="MDGIIPDKAYFRIGEVSKILNVEPYVLRYWETEFRTVKPVRTSS
                     AQRLYRKKDVEELLIIRNLLYQQRFTINGAKKQLMKIRSGEEAPPEAASEDKLVLIKK
                     ELQRIKKIMS"
     gene            complement(7494..7772)
                     /locus_tag="PLR47_03885"
     CDS             complement(7494..7772)
                     /locus_tag="PLR47_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxxKW family cysteine-rich protein"
                     /protein_id="HOG12111.1"
                     /translation="MLCETVKKGVECAFMSKKGCGFFGNSCQPVVDKCGECNKIFEFE
                     TGRYCKVYPDPKSKWLTGNCPTASHVKIEIKETQKTNPLKASKRANKK"
     gene            8013..8765
                     /locus_tag="PLR47_03890"
     CDS             8013..8765
                     /locus_tag="PLR47_03890"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HOG12112.1"
                     /translation="MKKGSLLKIFWRSFFIHAALNFRRMQNLGFAYAMISLLRQRKMP
                     DREAEEMLVRHLQRFSTHPYLSAPLLGSILRMEEDRPDGEDAAPITAVKQSLMGPYAA
                     IGDAFFWGSLRPCAGICAVGLAWLGCVFAPLVFLLIYSPAHLLVRGRGFFEGYRGGKQ
                     GIAAIRAMDLPGVTGRIRWLALAALIAGSLLVFQAGFRGASAFGVAAAMLAALLLCWW
                     LVKRGVSQTYILYGAAALFMIFSTRGLWLCWI"
     gene            8747..9022
                     /locus_tag="PLR47_03895"
     CDS             8747..9022
                     /locus_tag="PLR47_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOG12113.1"
                     /translation="MAVLDMKTFTLKNKLGMHARAAALFVRIAQKYRSDIVIERNGQT
                     VNGKSILGILTLACPMGGQITVSARGDDADGALAELEALIENKFGEE"
     gene            9032..>9605
                     /locus_tag="PLR47_03900"
     CDS             9032..>9605
                     /locus_tag="PLR47_03900"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HOG12114.1"
                     /translation="MMSDQGKKTFVLKGIGVSPGVVIGKVYRFDPLDAQISFYKLHSE
                     DMVPREIERFKNALKESSRQLLEIQENLKKTKVTEPLYIIDVHILLLSDKKFISRTIK
                     YIRRMGVNAEWALRMTLDHYKQIFEGLEDVYISGRISDVQYVIQRILRNLSGEKHEIV
                     WDVGQEGVVIVSHDLSPADTAQMKMDKIVGF"
BASE COUNT         2300 a         2480 c         2801 g         2024 t
ORIGIN      
        1 cctgacgcgg tggatgtcca gggttcaaat ccctgcttgc ctaccatttt ttatattcgc
       61 gcatatgccg ttcatgccga ttgaaagaaa agggcatcag caaaatggtg atgccctttt
      121 aaaatgatcc aaaacattaa agattttgat gaactcatgg atgtaaaagt aatttttccc
      181 gaccagtcgg aacagacctt tcccaggggc gtaaccgttt ccgaggccgt cggaagctgg
      241 aagaaagaag cgcttgccgc cgccgtcgca gccgacgtca acggtgtttc cgtcgatctg
      301 agttttgcgc tctcgggcga cgcaaaggtt gaaccaattg atatttcttc aaaaaaaggc
      361 atttccatat tgcgccacag cgtttctcac gtaatggcgc aggccgtgca ggaagtgttt
      421 cagggcgcga aagtcaccat cggcccttcc attgaagacg ggttttatta cgattttgaa
      481 tatgccgaac cgtttacgct ggatgatctt gagaaaatcg aaaaaaagat gaaggaaatt
      541 gtcgcggccg atcatccgtt tgtccgcgag gagctgcccc gcgaaaaggc ggcggagctt
      601 tttgccgcga agggcgaaga ctacaaggtg gagctgattc gggatctgcc ggcggatgtt
      661 tctgtggtca gcctctacaa agacggggat tatgttgatt tatgccgcgg cccgcacatt
      721 ccgcggaccg gcatgatcaa agccttcaag ctgctgagcg tcgcgggcgc ctactggcgc
      781 ggcgacgagc gaaacaagat gcttcagcgc atttacggca cgggctttgc cgacgagcag
      841 gcgcttcagg attatctgac gctgctggaa gaggccaaaa agagagacca ccggaaactg
      901 ggccgcgagc tggatctgtt tcaggtcaac gacgaagccg gggcggggct ggtgattttc
      961 catcccaagg gcatgctgct tcgctatctt attgaggaat gggaaagaaa agagcatttg
     1021 aagcgcggtt acgacatggt gatgggcccc cagattctca aggtggatct gtggaagaaa
     1081 tcgggccatt tcgatcacta ccgggacaat atgtatttca ctgaagtgga tgagcagact
     1141 tacggcatca agccgatgaa ctgcctgtcg cacatgctga tctacaaatc aaaaatccgc
     1201 agctaccgcg atctgccgct tcggtatttt gaactcggca ccgttcatcg ccacgaaaag
     1261 accggcgttc tccacggatt gatgcgcgta cgccagttta cgcaggatga cgcgcacatc
     1321 ctgtgcatgc ccgaacagct gaacgcggaa atccgcgcca tcgccgactt tgtccattac
     1381 gcgatgggca ttttcggctt tgaatatgaa gtggaattaa gcacgcggcc ggagcactcc
     1441 atcggatccg acgcggactg ggatctggcc acaaaggcgc tggaaaacgc gcttgtggac
     1501 aacaaaattc cctacgaagt gaacgagggc gacggcgcgt tttacgggcc gaaaatcgac
     1561 ttcaagctca aggacgccct gaagagaaaa tggcagtgcg cgacgatcca gtgcgatttc
     1621 accctgccgg agaggttcga tctgagctac atcggccccg acggcgaaaa gcaccgcccc
     1681 gtgatgctgc acagggtcat cctgggggcg atcgaacggt tcatgggcgt tctcatcgaa
     1741 cattacgccg gagcgtttcc ggtatggctg gcgcccaccc agtgcgttgt gctgacggtt
     1801 acggacaagc agattcccta tgccgaatcg gtgcgcggcc gattggtcga agccggcatc
     1861 cgttgtgagc cgtatttcga caacgacaag ctggggtaca aaatccgcca ggcgcagatg
     1921 cagaaggttc cctacatgct ggtcatcggc gacaaggaag tggcggcctg tgccgtggcg
     1981 ccgagagcgc gggacggccg gaatctgggc gcaatgcccg tggatgaatt cattgcgctg
     2041 attcaagaag cgtgcgggag caaaaagtag caatcgactt tgggaaggac aatccgggag
     2101 gtgccgtata gctaaggatt taaggatcaa ccgagaaatc aagtcggcga acgtcagagt
     2161 catcaacgaa gagggccagc cgctgggcgt gatcccgctg gaggaagcga tggcacaagc
     2221 ggaaagagcg gccctggatt tggtggaagt gtcggccaac gccgatccgc ccgtctgcaa
     2281 aatcatggac tacggaaagt accgctacaa gcagagcaaa aaaatccatg atgcccggaa
     2341 aagccagacg gtgattcatg tcaaggaaat ccgattgcgg cccaaaacgg aagcgcatga
     2401 tctgcaggtc aagatcaagc acatcaaaaa gtttttggag cagcgcgaca aggtaaaaat
     2461 atccatgatc ttccgggggc gcgaaatcgc ctttaccgag atcggccgca aaatcatgga
     2521 gcagatcaag gcggcgctgg ccgatgaatg catcatggac caggagccca ggctggaagg
     2581 ccgcagcatg gtgatgattg tttcaccgaa aaaataaatc cgaggggatt caaaaagagg
     2641 tgagaagatg ccgaaaatga aaacacatcg aggggcggcc aaacgttttt ccgtgaccgc
     2701 aaaggggaag gtcaagcgca gcaaggcgtt tgccagccac atcctcacga agaaaaccac
     2761 caaaagaaaa agaacgctga ggaaggccgc gctggtgcac ggagccaacg aaaaagccat
     2821 caaaaggctg attccgtatt tgtaggccgc cgggaaaaag gaagttgttt catatcgaag
     2881 gagagtgaaa gaaaatggct aggatcaaaa gaagcgtcac cgccaggaaa aaaagaagaa
     2941 aaatattaaa aatggccaag ggatttttcg gagcgcgcag ccgtctgctt cgaaccgcga
     3001 ccgaagccgt caacaaggcc atgagctatg cctatcgcga ccgcaggggc cgcaagcgcg
     3061 aatttcgcgc gctgtggatt gcgcggatca atgcagccgc ccgcctgaat caaatctcct
     3121 acagccgcct catggacggt ctgaagaagg cgaatatcgg tctggatcgc aaggtccttg
     3181 cggagctggc cgtgaacgac ccgcaggatt tcgcccgcat tgtggaaacg gctaaaggcg
     3241 agcaggcggc atgacagggg accttcaact tctggaaaag gatgctctgg ccgagctgga
     3301 aaaagccggc ggtgaggatt caattctggc ggtgagaacg aaatacctcg gccgcaaagg
     3361 ccttctcacc gggatgctga gaaatatttc ttccgtgccg gccgaagaaa gacgcgtctt
     3421 cggcatgcgc tgcaacgaag tgaaggagct tctggaaaaa aacatcgaag aagccctgcg
     3481 gcgcaaggcc gaggatcaaa aagccgaatg gctggccgcc cagcagatcg acgtcacgct
     3541 gcccggcaga ggcattcgcg cgggccgcgt tcacccggtg attcaaatcc gcaacgagat
     3601 ctgcgggatt ttcgccgctt tcggcttttc agtggtcgaa gggccggaag tcgagctgga
     3661 ctactacaac ttcgaagcgc tgaacattcc caaggatcat cccgcccggg atatgcagga
     3721 caccttttac attgaagaca acatggtcct gcgcacccat acctcgcccg tgcaggtcag
     3781 gatcatggag aaggcaaagc ctcccgttcg cattttgtcc ccgggtcggg tgtaccggcc
     3841 ggattccgat gtttcccaca cgccgatgtt tcatcagatt gaaggcttgc tggtggaccg
     3901 gggcgtcagt tttggcgacc tgaagggcat cctgaccgct ttcttgagga aagtgttcgg
     3961 ggaaggcacg atcctgcgct ttcgtcccag ttttttcccc tttaccgaac cgtccgcgga
     4021 ggtcgatatc cgctgcgtca tgtgcggcgg caaaggatgc cgcgtctgcg gacagtccgg
     4081 gtggctggag attctgggct ccggcatggt cgaccccgcc gtctttcaga acgtcggcta
     4141 cgacccggaa gtttataccg gttttgcttt cggtatgggg ctggagcgca tcgccatgct
     4201 taaatacggc atttccgaca tccgtcttct ttttgaaaac gacattcgat tcctgaaaca
     4261 gttttaggag aatgtgattg catgctggtc agtctcaaat ggcttaagga ttatatcgac
     4321 gtggaaacgg ctcccgagga gcttgcggac cggctgacca tggccggtct ggaggtggat
     4381 gagattcgca cgctctcccc cagattttcc ggcgtcgtgg ttgcgaaaat tttatccgtc
     4441 cggccgcacc ccaacgcgga caagctttcc ctgtgcgatg tcaccgacgg cgcgcagacc
     4501 tatcccatcg tctgcggcgc caaaaacatc catccgggcg acatcgttgc gctggcaaaa
     4561 atcggcgcaa tcatacccgg cggctacacg atcaaatcca ccacgctgcg cggagaaaaa
     4621 tccgaaggca tgctctgctc cgaggcggag ctggaaatcg gcgacgacgc ttcgggaatt
     4681 gtgcagttgc cggccggcat gacgccggga acgccgctgg aagaggccct ggacctgaac
     4741 gacacggttc tggacgtcgg cgtcaccccc aaccgttcgg actgcctgag catgatcggc
     4801 atggcccgcg aagcggccgc cctcaccgga aagaccctga aaatgcccga agggaaggtc
     4861 agggaagcgg ccgaagacgt gtcgctgctc tcgtcggtaa ccattgaaga tccggacctg
     4921 tgcccgcggt acacggcgcg gctgattcgg aacgtgacca tcggcgattc tcccgtctgg
     4981 atgaaaacga ggctcgaggc cgccggcttg cgcgccatca acaacgtggt ggacgtgacc
     5041 aacttcgtca tgctggaaat ggggcagccg ctccatgcgt tcgattttca cttcctggaa
     5101 gagaggcgca tcgtggtgcg caaatcaaag cccggcgagg aattcgtttc actcgacggg
     5161 aaaagccgca tccttcccga aaatacgctg ctgatttgcg acggaaaaaa gcctgtggcg
     5221 attgccggga tcatgggggg actgaattcc gaggtcaaag aagacacccg gacgatattt
     5281 ctggaaagcg cctatttcaa tccctcatcc attcgccgtt cgtcacgccg tctggccatg
     5341 ccgacggacg ccgctttccg ctttgaacgg ggcattgatc ccgaaggcgt tatccgggcc
     5401 ctggacaggg cggcacagct gatcgccgat ctttccggcg gcgaggtctg cgcgaaccgg
     5461 ctggacgaat acccggcgaa aatcaagccg gttgaaaaca tacttctgcg aatggaccgc
     5521 gccgggcagg tgatcggagt tgccgttccg gccagggagg ctgttcgcat tctgaagagc
     5581 atcggcatga cgctgaagcg cgagggcagg ggagtctatc gcgtgacgcc gccctcgttt
     5641 cgggtggata tcgaaagaga aatcgacctg atcgaggaaa tcgcgcgtct ttacggttac
     5701 gaccgggtgc cttcgacgct gccgtccgtc tcagtgacgg agctggacgc cattccccgc
     5761 ctggcgcttg aagagagaat tcgcctcctg ctcacgggct ttggctattc ggaaatcatc
     5821 aactacagtt tcacgacgcc cttttgcgcg gaagcgctcg gcctgccgcc ggaagatgaa
     5881 aggcgacggt tcgttgtcat ccggaacccg ctgacggaag accagtcggt catgcgcacg
     5941 acgctgacat acggtctgct ggaaaccctg aagaagaaca tcaacaatgc ctcttggaat
     6001 ctgaagctct ttgaaatagg acggactttc ctcaggcgcg gcgatggcga gctgccggaa
     6061 gaaagaaata tcctggcggc gctcgccgcg ggcaaggcat cggatgacct ctggggattg
     6121 aaagccggtg ttgactttta tgatctgaaa ggctgcctgg aaaatatttt tcaggatttg
     6181 aagttcgact ccatgcggtt ccgggcggaa aagccccagt cgtatctgca ccccggtcag
     6241 tcctgcggcg tgtatgcgga tgatgtccgg atcggctcca tgggaaaagc gcacccggat
     6301 gttctgaaaa aaatggacat caagagtgac gcttatgtat ttgaaattga tcttgatctg
     6361 ctgggcgcaa agatacggcc gcgggttcgc tgcagggaaa tatccaagtt tcctgcggtt
     6421 ttgcgcgatg ttgcttttgt tcttccctcg tccatggaat ccgaaaaaat gcttgaaatg
     6481 gttttgcgtc agcgtgaaga tttgcttgaa aatgtttcaa tttttgatat atacgcgggc
     6541 aaaggtcttg aagaaggaac caagagcctg gggctcaggt tttcctaccg cgctttggac
     6601 agaacgttga cggatgcgga agtcagtgcc attcacgacc gaattgtgaa ggacacgatt
     6661 cggctgacgg gtgcaaaaat aagagcctaa cagtttacat gacgttaaaa cggagggatg
     6721 gacatgacaa aaattgatat tatacagaat gtttacgaaa aactcggctt ttccaaaaaa
     6781 gaatcttcag atatcgttga gtccgtattt gacatcatta aagacagcct tgccgcgggc
     6841 gaacgggtga aaatatcggg tttcggcaat ttcatggtaa aggaaaagcg cgcgcgccgc
     6901 ggccgcaatc cccagaccgg cgaggaaata tccattacag cgcgccgggt tttgactttc
     6961 aaatcctccc aggtgctgcg taaatcttta aacagttagt gcaacgtttt ttctgcgccg
     7021 ccggcgccgg aaagactgcc ggctgatttg cggcgggaac ctttttgcgg tttttcgcct
     7081 gttcgtgtat ttctccgcaa gagctcagga atcgtatgga cggcatcatt cccgacaaag
     7141 cctatttccg catcggggaa gtgagcaaaa tcctcaatgt tgaaccgtat gtccttcgct
     7201 actgggagac cgaattcagg accgtcaagc cggtgcgcac cagctccgcg caaaggctgt
     7261 accggaaaaa agacgtggaa gaattgctga tcatccgcaa cctgctctac cagcagcgtt
     7321 ttacgatcaa cggcgccaaa aaacagctca tgaaaatccg ctccggagag gaagccccgc
     7381 ccgaggccgc ttccgaggat aagctcgtcc tgatcaaaaa ggagctccag cggattaaaa
     7441 agataatgag ctgaaaaagc cggctttcgg atttcttctg cggaacaaaa agattacttc
     7501 ttgttggctc ttttagaagc tttcagcgga ttggtctttt gcgtttcctt gatttctatt
     7561 ttgacgtgcg acgcggtcgg gcagttgccg gtcagccact tgcttttggg gtccggatag
     7621 accttgcagt accggccggt ttcaaattcg aaaattttgt tgcattctcc gcatttatca
     7681 acgacaggct gacagctgtt cccgaaaaaa ccgcagccct ttttggacat gaaggcgcat
     7741 tccactccct ttttgactgt ttcgcaaagc atgttgcaca aaccccctta cctgaatttt
     7801 ctgtaatgtt gaacgccgcg gaaagcggcg gccgaactgg tcttgaaaac cggcgtgtcc
     7861 ttaacagcaa aaaaaccggc tgtcaagaaa aaataagaaa aattgaagct tgcgggagcc
     7921 ctgattaaat tgacacaacc ctgcatttat gttagttcct tcaccgcgta aaagcaggga
     7981 aataatcaag atatgcgcga cgggggaagg gaatgaaaaa gggctccttg ctgaaaatct
     8041 tctggaggtc ttttttcatt cacgcggcgc tgaactttcg ccgaatgcag aacctcggct
     8101 ttgcgtacgc gatgatttcg ctgctcagac aacggaaaat gcccgatcgc gaagcagagg
     8161 agatgctggt cagacacctg cagaggttca gcacgcatcc ctatctgtcg gcgccgcttc
     8221 tgggctcgat cctgcggatg gaggaagacc ggccggacgg tgaagacgca gcgcccatca
     8281 cggccgtgaa gcagagcctg atggggccgt atgccgcgat cggcgacgct tttttctggg
     8341 gatcgcttcg gccctgcgcg gggatctgcg ccgtcggact ggcctggctg gggtgcgttt
     8401 ttgcgccgct ggtgtttctc ctgatctatt cgccggcgca cctgctcgtg agagggcggg
     8461 ggttctttga aggctaccgc ggcgggaagc agggaatcgc ggcgattcgc gcaatggatc
     8521 ttccaggcgt gacaggccgc attcgctggc tggccctggc ggccctgatc gcgggcagcc
     8581 tcttggtttt ccaggccggt ttccggggtg cgtctgcgtt tggcgttgcg gcggccatgc
     8641 tcgccgcgct tttgctctgc tggtggctgg tgaaacgggg cgtatcgcaa acctacattc
     8701 tgtatggcgc cgcggcgctt tttatgattt tttcgacaag agggttgtgg ctgtgctgga
     8761 tatgaaaacc tttaccttga aaaacaagct gggcatgcat gcgcgcgcgg ccgcgctgtt
     8821 tgttcggatt gcccagaaat accgctcgga tattgtcatt gaacgcaacg ggcagaccgt
     8881 caacgggaag agcattctcg gaattctgac gctggcctgc ccgatgggcg gccagattac
     8941 cgtcagcgcc aggggcgatg acgccgatgg agcgcttgcc gagctggagg cgttgattga
     9001 aaataaattc ggagaagaat aaaaacagca gatgatgtca gaccagggaa agaaaacttt
     9061 tgttctcaag gggataggcg tttccccggg cgtggtgatc ggcaaggttt accggtttga
     9121 cccgctggac gcgcaaatat cattttacaa gctgcacagc gaggatatgg ttccgcggga
     9181 aattgagcgc ttcaaaaacg cgctgaaaga gtcctcgcgg cagcttctgg aaattcagga
     9241 aaacctgaag aagacaaaag tgacggagcc gctctatatc atcgacgtcc acattttgct
     9301 tctctcggat aaaaaattca tcagccgtac gatcaaatac atccgccgca tgggcgtcaa
     9361 cgcggaatgg gcgctgcgca tgaccctgga ccattacaag cagatttttg aaggccttga
     9421 ggatgtttat atcagcggcc gcatcagcga cgtgcagtat gtgatccaga ggattctgcg
     9481 caatctttcc ggcgaaaaac acgaaattgt ctgggatgtc ggacaggaag gcgtcgtgat
     9541 tgtctcccat gacctgtccc ccgccgacac ggcgcagatg aagatggata aaatcgtcgg
     9601 ttttg
//
LOCUS       DAOSIM010000055         9591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_6869_length_9591_cov_8.363674, whole genome shotgun sequence.
ACCESSION   DAOSIM010000055 DAOSIM010000000
VERSION     DAOSIM010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9591
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6869_length_9591_cov_8.363674"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /gene="murI"
                     /locus_tag="PLR47_03905"
     CDS             complement(<1..749)
                     /gene="murI"
                     /locus_tag="PLR47_03905"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOG12115.1"
                     /translation="MNIQANQAIGVFDSGIGGLTVVRSLMERLPFENIIYFGDTARVP
                     YGVKSVETIRRYALQIAEFLLKQDVKLLIVACNTMAAVAYQAIRDLSPVPVLEVIEAS
                     AKIAVARTRSKSIGVIGTPATINSNAYARAIHILDRDAKVFSQACPLFVPLVEEGWFE
                     HPATRLVAEEYLKPVMAERVDTLVLGCTHYPLLKPLFQDITGGQVRLIDSAEAMADIA
                     ADLIRQNNLGSTANRPPEYLFCVSDVPYRFQT"
     gene            993..1394
                     /locus_tag="PLR47_03910"
     CDS             993..1394
                     /locus_tag="PLR47_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12116.1"
                     /translation="MLNKIVIRYTDKRLVKGVTVDFAPNRDIFHVTPAGFSPSDKPLE
                     VRVADLKAIFFVKDFSGNPKHEDRKNFEPGIPVLGRKIRVLFKDGEVLVGTTQGYQLN
                     RQGFFVIPADAQSNVERCFVVTKATREVKFI"
     gene            1456..1794
                     /locus_tag="PLR47_03915"
     CDS             1456..1794
                     /locus_tag="PLR47_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12117.1"
                     /translation="MPVYIQIILWVVGFAVFVLAVMYFAGFGLRRVCFKIIAELEEAG
                     AFKVSRAIRLQDERKNFFRVGTGNLRPRALHLLITDKLVVKTPDGKYYLDREKLAELK
                     SGDGKSSSGS"
     gene            complement(1781..2143)
                     /locus_tag="PLR47_03920"
     CDS             complement(1781..2143)
                     /locus_tag="PLR47_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12118.1"
                     /translation="MKKISLALMAAALLISGLPPAQAQEYGKVRALQERAAYVIRQKN
                     DFVVRVLRSYKIPHEVNDQGVVVRINMNDRWMDVTAIEIVPMLREAEDRSRQVAAHEL
                     FFFTADGILDVVSALTIR"
     gene            complement(2203..3888)
                     /gene="ettA"
                     /locus_tag="PLR47_03925"
     CDS             complement(2203..3888)
                     /gene="ettA"
                     /locus_tag="PLR47_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOG12119.1"
                     /translation="MANEGNKVIYSMIGVSKVYEKKPVLKDIYLSYFYGAKIGVIGLN
                     GSGKSSLLKILAGVDAEFLGKTILSAGYTVGYLEQEPRLDEAKTVRRIVEQGVQATID
                     VVNDYNAVNEMFAQEMSDEEMAKLCDRQAALQEKMDALDAWDIDSRLEMAMDALRCPP
                     GDTPVQVLSGGERRRVALCRLLLQKPDILLLDEPTNHLDAESVAWLEHHLQKYEGTVI
                     AVTHDRYFLDNVAGWILELDRGQGIPWQGNYSSWLEQKQNRLKNEEKTESARVKTLER
                     ELEWIRMSPKGRHAKSKARIKAYEELLDRNLEKESGTRELFIAPGPRLGDLVIEADAV
                     SKGYGDKLLVDAMTFKLPPGGIVGVIGPNGAGKTTLFRMITGREMPDAGVIRIGETVK
                     LAYVDQSRDALDPEKNIWEMISDGQDIIPVGGRQINSRAYVSRFNFSGTDQQKKVGTL
                     SGGERNRVHLARMLKEGGNVLLLDEPTNDLDVNTLRALEDALESFAGCVVVISHDRWF
                     LDRICTHILAFEGDSKVLFFDGNYSEYEEDRKSRLGAAASQPHRIKYRQLTRR"
     gene            complement(3921..4586)
                     /locus_tag="PLR47_03930"
     CDS             complement(3921..4586)
                     /locus_tag="PLR47_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG12120.1"
                     /translation="MKYREDIYVYEWANYFDNNCNSYYIGGNVKALVDPGLTRYLPDL
                     LNQMANDGIRKKDIQYVINTHSHPDHFQGSELFDHEKVKIALHRKEMDFLKGIGGELY
                     GLFGMTVPQMQINMQLEDGRLELGGGLFKVISIPGHTPGSIGLYWPAKKALFSGDVVF
                     QQNVGRTDFPGGNGAHLKKSIVTLSDLDVEMLFPGHMSIIEGHDKVQDNFNIIIQNIF
                     PYI"
     gene            complement(4639..4854)
                     /locus_tag="PLR47_03935"
     CDS             complement(4639..4854)
                     /locus_tag="PLR47_03935"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOG12121.1"
                     /translation="MTTDNWFAEFAKSETDRWIGGVCGGLGEHTPIPSWVWRLLFTLL
                     VLCFGTGFLLYILLWIFVPRRAANRPG"
     gene            complement(4936..5442)
                     /locus_tag="PLR47_03940"
     CDS             complement(4936..5442)
                     /locus_tag="PLR47_03940"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOG12122.1"
                     /translation="MQKIYRVFVALFLILGLSAIPSAGQFIPNAPFKPQYTPAPDFTL
                     KDLKGKIFRMSAQRGKPVLIFFGTTWCPGCRAEIPKYKKVYETYASRGLEVIYINIME
                     PAKKVARFAQANALPYRTLLDEDGSVGALYNAVGVPMMILVDKDGNMIKVGFSSTQMP
                     LDKVTPVQ"
     gene            complement(5454..6167)
                     /locus_tag="PLR47_03945"
     CDS             complement(5454..6167)
                     /locus_tag="PLR47_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOG12123.1"
                     /translation="MIENLINNLALYIQGSVILAFAAAYLGGVVISFTPCTYPLIPVT
                     VGFIGAQKATTKLRGFLLSLFYVLGLSVTYAALGAVAALSGRLFGQMQTTPLVYFLMA
                     NVCLVMGLAMLDVFKISIPIPQKILNYTGGGKKGFLPSFLLGAASGFVIGPCTAPALG
                     VLLGFVALKTHVAMGIGLLFVFAFGMGTLLILVGTFAGLIAALPRSGAWMTVITRVFG
                     VILIGAAEYFLYTAGTLAF"
     gene            6688..8133
                     /locus_tag="PLR47_03950"
     CDS             6688..8133
                     /locus_tag="PLR47_03950"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HOG12124.1"
                     /translation="MGSKQGLFKTEDWWSVWLGLFIFIISLGSLLGLDLLGWAVTPKV
                     WMEISKSIAPASKGYAELHPIISIILTFLAVLGLLMVGARAMGYDLKKFVYGFTIIFW
                     LTYCCNIIGHYAVIAAQTPADLKKFGLDWSLKLTGEAGLILALLVGLFIGNFMPKFAN
                     SLKEAARAEWFIKTAIVIMGVGLGVKSAEQLGLATSMMFRGLCAIVEAYLIYWAVVYL
                     IARKYFKFNREWAAPLASGISICGVSAAIATGGAIRARPIIPVMVSSLVVIFAVVELI
                     ILPFVATELLPNQPLVAGAWMGLAVKTDGAAVASGQIVESLIYAKNAAQGINYQPGWV
                     VGAATTVKIFIDIFIGIWAFILAVIWTTKINPTKGDKARAGEIWDRFPKFVIGYFVTF
                     IIMLIIGLNWPDIKSQIKISASETDVFRKLFFALTFFSIGLASNFKKLWEEGIGKLML
                     VYVIALFGFIIWVGLFISWIFFQGVMPPLVQ"
     gene            8156..8314
                     /locus_tag="PLR47_03955"
     CDS             8156..8314
                     /locus_tag="PLR47_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12125.1"
                     /translation="MDDVKIKSEMEKMEYEPLLPIEKKLITWSLILGVVLLVGLVWVS
                     YTFFPAGQ"
     gene            8645..>9591
                     /gene="dsrA"
                     /locus_tag="PLR47_03960"
     CDS             8645..>9591
                     /gene="dsrA"
                     /locus_tag="PLR47_03960"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - hydrogensulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     alpha"
                     /protein_id="HOG12126.1"
                     /translation="MANSETPLLDELEKGKWPSFVKDLKQAAGKNPMARDLIHQLELS
                     YKDRIGHWKHGGIVGVKGYGSGVIGRYSDRPDLFPNVQAFHTMRVNHPTGWFYKTDTL
                     RKLCDIWDKHGSGLTNMHGSTGDAILLGTTTDHLQSCYDDLSNAGFDLGGSGSALRTP
                     SACVGPARCEWACIDSLDLCYNLTMTYQDQMHRPMFPYKFKIKISACANDCVAAIARS
                     DLSVIGTWKDDIQVDQKEVLAYASSINIQKEVCDLCPTRCMEWNGKELKIYDEDCTRC
                     MHCINLMPKALRPGKERGASILVGGKAPIVRGALLSWVIV"
BASE COUNT         2174 a         2592 c         2603 g         2222 t
ORIGIN      
        1 gtctgaaaac ggtacggcac atcgctgacg caaaaaagat actccggcgg ccggtttgcc
       61 gtgctgccca gattgttctg ccgtatgagg tcggcggcaa tgtccgccat ggcttccgcc
      121 gaatcgatca gccggacctg cccgccggta atatcctgaa agaggggctt gaggagcgga
      181 taatgtgtgc agccgaggac cagcgtgtcc acgcgctcgg ccatgacggg cttcaggtat
      241 tcctcggcga cgagcctggt ggccggatgc tcgaaccagc cttcctccac cagcggaacg
      301 aagagggggc aagcctgcga aaaaaccttc gcgtcccggt ccaaaatatg gatggccctg
      361 gcgtacgcgt tcgagttgat cgtggcgggt gtgccgatca caccgatgga tttgctgcgc
      421 gttcgggcga cggcgatttt cgcgctggcc tcgatcactt ccagcaccgg cacgggcgag
      481 agatcccgaa tggcctgata ggcgaccgcc gccatggtgt tgcaggcgac gatcaggagc
      541 ttgacgtctt gcttcaacag gaattcggcg atctgtagcg cgtagcgccg gatggtttcc
      601 accgatttga cgccgtaagg cacccgcgcc gtatccccga agtaaataat gttttcaaaa
      661 ggcaggcgtt ccatgaggga acgaaccacc gtcagtccgc cgatgccgga atcaaaaacg
      721 ccgattgcct gattggcctg aatgttcatg ttgtccctta tccccgtcat ttcgtcatcg
      781 acattttttg ccgtttcgga taaaagcagt atccggattc ggaaaagtcg acgaaaaagc
      841 gggctttttg taccacaact ttccgaaaca tgccaagagt tcttcaaacg atgcactgga
      901 caaagttcca tcggttggtg taagcaaagc aacgatgtcc ggtaaaaaat cgcgttgaaa
      961 cgcggctcac gccggaaagg aacccagccg ccatgttaaa caagatcgtc attcgctata
     1021 ccgacaagcg tcttgtcaaa ggcgtcacgg ttgactttgc ccccaaccgg gatatttttc
     1081 atgtcacgcc ggcgggattt tcgcccagcg acaagccgct ggaagtgcgc gtcgccgatc
     1141 tgaaagccat ttttttcgtc aaggatttct ccggcaatcc gaaacatgag gaccggaaga
     1201 attttgagcc gggcataccc gttttgggcc gcaagatccg ggtgctcttc aaggacggcg
     1261 aggtcctggt gggaaccacc caggggtatc agttgaaccg acaggggttt ttcgtcattc
     1321 cggcggacgc tcaatccaat gtcgagcgct gctttgtggt cacgaaggcg acgcgtgaag
     1381 tgaagttcat ctagcggcaa tgcaaagaat ccggatgcgc gggatcctcg cttccggcag
     1441 gatgaaaata gtcatatgcc tgtttatata caaattattc tgtgggtcgt gggctttgcg
     1501 gtcttcgtcc tggcggtgat gtatttcgcg ggtttcggcc tgcggcgggt ctgcttcaaa
     1561 atcatcgccg aactggaaga ggcgggcgcc ttcaaggtgt cccgggccat ccggcttcag
     1621 gatgagcgga aaaatttctt tcgggtcgga acgggcaatt tgcgcccccg ggcccttcat
     1681 cttctgataa cggacaaact ggtcgtcaaa acgccggacg ggaaatacta cctggaccgg
     1741 gaaaagctag cggaactgaa aagcggcgac ggcaagtctt ctagcggatc gtgagcgccg
     1801 acaccacgtc cagaatcccg tccgccgtaa agaaaaacag ctcgtgcgcg gccacctgcc
     1861 ggcttcggtc ctcggcttcc ctcaacatgg gaacgatttc gatggccgtg acgtccatcc
     1921 accgatcatt catgttgatg cgaacgacaa caccctgatc attgacctca tgcggaatct
     1981 tgtaggaacg caaaacccgt acgacgaaat catttttctg ccggatcaca taggccgccc
     2041 gctcctggag cgcgcggacc tttccgtatt cctgcgcctg cgccggcggc aaaccggaaa
     2101 tgagcagagc cgccgccatc aatgccaaac ttattttttt catttccacc tccctctcaa
     2161 ggaacagggc gccgcacggc ggcgggtctg cgcgtcccgc ggctaccttc tggtgagctg
     2221 acggtatttg atccggtggg gctggctggc cgccgcgccc agccggcttt tgcggtcttc
     2281 ttcgtattcg gaatagttgc cgtcgaaaaa cagcactttg ctgtcccctt caaaggccag
     2341 gatgtgcgtg cagatgcggt ccaggaacca gcggtcgtgg ctgatcacaa cgacgcagcc
     2401 ggcgaaactc tccagcgcgt cttcgagcgc ccgcagcgtg ttgacgtcga ggtcgttagt
     2461 cggctcgtcc agcagcagaa catttccgcc ttccttgagc atgcgcgcca ggtggacgcg
     2521 gttgcgctcg ccgccggaaa gcgttccgac ctttttctgc tgatccgttc cggagaagtt
     2581 gaaccgcgag acgtaggcgc gggaattgat ctgccggccg ccgacaggga tgatgtcctg
     2641 gccgtccgaa atcatctccc agatgttttt ctccggatcc agcgcgtcgc ggctctgatc
     2701 gacatacgcc agtttgacgg tctcgccgat gcggatcacg cccgcatccg gcatctcccg
     2761 gccggtaatc atgcggaaga gcgtggtctt gcccgcgcca ttggggccga tgacgccgac
     2821 aatgcccccg ggcgggagtt tgaaggtcat ggcatccacc agcagtttgt cgccgtatcc
     2881 tttggagaca gcatcggctt caatcaccag atcgcccagg cggggaccgg gcgcgataaa
     2941 gagttcgcgc gtgccggatt ctttttccag attcctgtcc agcagttcct catacgcctt
     3001 gatgcgcgcc ttggatttgg catgccttcc tttaggcgac attcgaatcc actccagctc
     3061 gcgctccagg gttttgacac gggcgctttc cgtcttttcc tcgtttttca ggcggttttg
     3121 cttttgttcc agccaggagg aataattgcc ctgccagggg atgccctggc cgcggtcgag
     3181 ttcgagaatc cagcccgcga cgttgtccag aaaatagcga tcgtgcgtca cggcaatcac
     3241 cgttccttcg tatttttgca ggtgatgctc cagccaggcc acggactcgg cgtccaggtg
     3301 gttggtcggt tcgtcgagca gcagaatatc gggcttctgc agcagcaggc ggcacagggc
     3361 cacgcggcgg cgttctccgc cggaaagaac ttgcacgggc gtatcgccgg gcggacagcg
     3421 cagcgcatcc atggccatct ccaggcgcga atcgatatcc caggcgtcga gcgcgtccat
     3481 cttttcctgc agggcggcct gccggtcgca gagcttggcc atctcctcgt ccgacatttc
     3541 ctgggcgaac atttcgttga ccgcgttgta gtcgttcacc acgtcgatcg tggcctggac
     3601 gccctgctcc acgatccggc gcacggtttt ggcctcgtcg agccggggct cctgctccag
     3661 gtagccgact gtgtagccgg cggacaaaat ggttttgccc agaaactccg catcgacgcc
     3721 ggccagaatt ttcaaaagcg agcttttgcc cgatccgttg aggccgatca cgccgatttt
     3781 cgcgccgtaa aaataggaca ggtaaatgtc cttgagcacc ggtttctttt cgtaaacctt
     3841 gctcacgccg atcatggaat agatgacttt gttcccttca ttggccatgc gccgccgatc
     3901 ctcccgcgaa ttgagaactc ctaaatgtag gggaatatgt tctggatgat aatgttgaag
     3961 ttgtcctgaa ccttgtcgtg gccctcgatg atgctcatgt ggccggggaa gagcatttca
     4021 acatccagat ccgaaagcgt cacgatgctt tttttaagat gcgcgccgtt gccgcccgga
     4081 aaatcggtgc gcccaacatt ctgctgaaag acgacatcgc cggaaaaaag cgctttcttt
     4141 gcaggccagt aaagtccgat ggagccgggc gtgtggccgg ggatggaaat gaccttgaaa
     4201 agcccgccgc ccagttcgag ccggccgtcc tcgagctgca tattgatctg catctgcgga
     4261 acggtcatgc cgaaaaggcc gtaaagttcg ccgccgatgc ccttgagaaa atccatttcc
     4321 ttgcggtgca gggcaatctt caccttttca tggtcgaaaa gctcggaccc ctggaagtga
     4381 tcggggtgcg aatgcgtgtt gatgacatat tggatgtctt ttttcctgat gccgtcgttg
     4441 gccatctggt tgagcaaatc cggcaggtag cgcgtgagcc ccggatcgac gagcgccttg
     4501 acgttgccgc cgatatagta gctgttgcag ttgttgtcga aataattggc ccattcgtaa
     4561 acataaatgt cttcccggta cttcatttta cacactttcc cctttaggta aatttatgca
     4621 gttgtttttt cccgaacgtc atcccggccg gtttgcggcg cggcgcggaa caaagatcca
     4681 aagcagaata tagagaagaa atcccgtgcc gaaacacagc accagcaggg taaacaacag
     4741 ccgccacacc caggaaggaa tgggcgtatg ctctcccaaa ccgccgcaga cgccgccgat
     4801 ccagcgatcg gtttcggatt tggcaaattc ggcaaaccag ttatccgttg tcatgggtcc
     4861 atccttgatc cggggtaaat gacgtcgggg gctccgcttg cggagtcagg cgccgaagca
     4921 aagcacgcag gtatcttatt gcaccggcgt cactttgtcc agcggcatct gcgtggagct
     4981 gaaaccgacc tttatcatat tgccgtcctt gtccaccagg atcatcatgg gcacgccgac
     5041 cgcgttgtac aaggcgccga cggagccgtc ctcatcgagc agcgtccgat agggcagagc
     5101 gttggcctgg gcgaaccgcg ccactttttt ggccggctcc atgatgttga tgtaaatcac
     5161 ttccagcccc ctggacgcat aggtttcata aacctttttg tatttcggga tctccgcccg
     5221 gcagccgggg caccaggtag tcccgaaaaa aatcaggacg ggtttgccgc gctgggcgct
     5281 catgcggaat attttcccct tcagatcctt cagggtgaag tccggcgcgg gcgtatattg
     5341 cggcttgaaa ggcgcgttgg gaataaattg cccggcagac ggaatggcgg aaaggccgag
     5401 aatcaagaac aaagccacga aaacgcgata tatcttttgc atgatgctcc ttgttaaaat
     5461 gccagtgtgc cggcagtata cagaaaatat tcggccgcgc caattaaaat aacgccgaaa
     5521 acccttgtaa tgactgtcat ccacgcgccg gaccgcggca gcgcggcgat cagtccggca
     5581 aacgtcccga ccaggatcag aagcgttccc atgccgaagg cgaacacaaa caacaggccg
     5641 atgcccatcg ccacgtgggt cttgagggcg acgaatccca gaagcacgcc gagcgccggc
     5701 gcggtgcagg gaccgatgac gaagccggaa gcggcgccca aaagaaaact ggggagaaat
     5761 ccttttttcc cgccgcccgt gtagttcagg attttttgcg gaatcggaat cgatattttg
     5821 aaaacgtcca gcatggcgag ccccatgacg aggcagacat tggccataag gaaataaaca
     5881 agcggcgtcg tctgcatctg accgaagagc cggcctgaca gagcggcaac tgcgccaagc
     5941 gccgcatacg tcactgaaag ccccagaacg taaaacagcg acagcaagaa gccgcgcagc
     6001 ttcgtcgttg ctttctgggc gccgatgaat cccaccgtga cggggatgag cgggtatgtg
     6061 cagggcgtga agctgatcac cacgcctccc aggtaggcgg ccgcaaaagc caggatcacc
     6121 gagccctgta tgtacaaggc taaattgttg atcagatttt caatcatctg ttttccatga
     6181 attgtttttt tagaatgtat agacggacga aaaaaacgtc aagataaaat atcggccacg
     6241 cctgtatttt cacctatatt ttcacctaca ttgtcgccta tattttctgt cgcctatatt
     6301 ttcttgacat gcaagattga gtcttttata ctccgaatcg taaacaacga ctcaaaaagg
     6361 atgctgcgat ccccgaactc aacatccctt tggagatgtc gcatgacgat tcctgtggca
     6421 tcgatattca gcggtaaatt tgaattcgat cttgcccttc ctgcggcttt tcataatcct
     6481 ttcagctcca tcagcaatct ccgcctccat atcttaaaca tcctcagcaa tgaaccggaa
     6541 acgaaaaatt gtcctcgctc aaagttcaat taacaatggt tttaggtctg aatgattaaa
     6601 atgcttcttt aacgataatt tgggaggggg ggtgaaacat taccggtcgt atttcagaac
     6661 taaaaatcaa tgaaaaggga ggtctctatg ggcagcaagc aaggtctttt caagaccgag
     6721 gactggtggt cagtgtggct gggattattt attttcataa tctcacttgg ctcgctttta
     6781 ggcctggatc tcttaggatg ggccgtaacg cctaaagtgt ggatggaaat ttcaaaatcc
     6841 atagcccccg catccaaggg ctatgcagaa ctccatccga tcatatccat cattttgaca
     6901 tttcttgccg ttctgggatt gctcatggtc ggcgcaaggg ccatgggtta tgatttgaaa
     6961 aaattcgttt atggctttac gattattttc tggttgacct attgctgtaa tatcattgga
     7021 cattatgcgg tcatcgccgc gcagacaccc gctgacctga aaaagttcgg actggactgg
     7081 tcattgaagc tgaccggtga agccggattg attctggctt tgctggtcgg tctttttatc
     7141 ggcaacttta tgccgaagtt tgccaactca ctgaaagagg cggcaagggc ggaatggttt
     7201 atcaaaacag ccatcgttat tatgggcgtc ggacttggcg tgaaatccgc cgaacaactg
     7261 gggctggcga catcgatgat gttcagagga ctttgcgcca ttgtcgaagc ctatctgatt
     7321 tattgggctg tcgtctattt aatcgccaga aaatatttca agttcaaccg tgaatgggcg
     7381 gcgccgcttg cttccggcat atccatctgc ggcgtgtcgg cggccattgc cacaggcggc
     7441 gccatccgcg cccgtccgat cattcccgtc atggtttctt ctctggtcgt tatttttgcc
     7501 gtcgttgaac tgatcatcct tccgtttgtc gcaacagaac tgcttcccaa ccagccgctg
     7561 gttgcagggg catggatggg ccttgccgtt aagacggacg gagccgccgt cgccagcggc
     7621 cagatcgtgg aatctctgat ctatgccaaa aacgcggccc agggcatcaa ttatcagccg
     7681 ggctgggttg taggcgcggc cacgactgtt aaaattttca tcgacatttt catcggcata
     7741 tgggcgttca tcctggccgt catctggaca accaaaatca atcccaccaa gggtgacaaa
     7801 gccagggccg gagaaatatg ggaccgcttc cccaaatttg tcatcggcta ttttgtgact
     7861 ttcatcatca tgctcattat cggtttgaac tggccggaca tcaagtcgca aataaaaatt
     7921 tccgcctcgg aaaccgacgt attccgaaaa ctgtttttcg ctttgacttt cttctccatc
     7981 gggctggctt ccaactttaa gaaactctgg gaagaaggca tcggaaaact gatgctggtt
     8041 tatgtgatcg cgctgttcgg tttcatcatc tgggtagggc tgttcatatc atggatattc
     8101 ttccagggtg tgatgccgcc gctggttcaa taattcacac tgaaaggagc ttatcatgga
     8161 tgatgttaaa attaagagcg aaatggaaaa aatggaatat gagcccctcc tgccaattga
     8221 aaagaaattg atcacctgga gtctcattct cggagtggtt ctgcttgtcg gactggtttg
     8281 ggtaagttat acatttttcc ctgccggcca ataaactacc tcgcagcaag ctacgaggta
     8341 tctatagcgg caaggaacga agcaagcttc ggggaatatg acccggcgag attaaaacgt
     8401 aacggttcat cagtatcgag catgactggt ttttgctcat caaatttgag taaaagccag
     8461 tcatgcctat tattttttta tatatttttc agatgcttgg atagtgttta acaattttcc
     8521 gaaaaacata acaagaatac ttgacacatc tcataaaaag agtactattc gaattgtgag
     8581 tagtgctatt gagcagaaag tctatgctcc taagttataa aatgcgttga tgaaggaggc
     8641 aaaaatggca aattcagaaa ctccgctcct ggacgagctg gaaaaaggaa agtggccaag
     8701 ctttgtgaag gatttgaagc aggccgccgg aaaaaatccc atggccaggg acctgatcca
     8761 tcaactggag ctttcctata aagacaggat cgggcattgg aaacacggag gcattgtagg
     8821 cgtcaagggc tacggttcgg gagtcatcgg gcgctattcg gaccgcccgg atctatttcc
     8881 caacgtgcag gcgtttcata ccatgcgcgt caatcatccg acgggctggt tttacaaaac
     8941 ggacaccctg cgcaaactct gcgacatctg ggacaagcac ggcagcgggc taaccaacat
     9001 gcacggctcc acgggggacg ccatactgct cggcaccacc acggatcatc tccaatcctg
     9061 ctatgacgat ctgtcgaatg ccggtttcga tctgggcggc tccggttcgg cgcttcgcac
     9121 ccccagcgcc tgcgtgggtc cggcgcgttg cgagtgggcc tgcatcgaca gcctggatct
     9181 gtgctacaat ctcacgatga cctatcagga tcagatgcac cggccgatgt ttccctacaa
     9241 attcaaaatc aagatttccg cctgcgccaa cgactgcgtg gccgcaatcg ctcgctccga
     9301 cctttcggtc atcggaacct ggaaagatga cattcaggtt gatcagaaag aagtcctggc
     9361 ctacgcctca agcatcaaca tccaaaagga agtctgcgat ctgtgcccca cccggtgcat
     9421 ggaatggaac ggcaaagagc tgaagatata cgatgaagac tgcacccgct gcatgcactg
     9481 catcaatctg atgccgaaag ccctgcggcc ggggaaggaa agaggcgcgt ccattctggt
     9541 cggcggcaaa gcgcccattg tccggggcgc cctgctctcc tgggtgatcg t
//
LOCUS       DAOSIM010000056         9371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_7024_length_9371_cov_8.728210, whole genome shotgun sequence.
ACCESSION   DAOSIM010000056 DAOSIM010000000
VERSION     DAOSIM010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9371
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7024_length_9371_cov_8.728210"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3916
                     /gene="rpoB"
                     /locus_tag="PLR47_03965"
     CDS             <1..3916
                     /gene="rpoB"
                     /locus_tag="PLR47_03965"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOG12127.1"
                     /translation="VSYKIGDVRYDMKECIQKGMTYAAPLKIVVRLVVFDTDRLADGR
                     ISRETSETKPIRDIKEQEIYFGEIPLMTENGTFIVNGSERVIVSQLHRSPGIFFDHDK
                     GKTVASGKLIYSARVIPIRGSWLDLEFDSKDLLYVRIDRRRKMPVTILLKAMGNSTEF
                     ILNYFYQIEKITLEGEKIYFGVDESLAGQKAPEDIKDPKSDEVIVKKGRKLTKLLLKK
                     IMDAGIKRVAIKEADLTGKILSSDVRDPGTGEILFRCNEELPVNGLEIAQEKGVRELT
                     FIHIDEDLDNASIRDTLLMDRIDSEEEAIMEIYRRLRPSNPPTPETATKFFKSLFFEL
                     ETYDLSDVGRAKMNYKLRLNVPTDLTVLRNEDILASVKYLIDLKNGVGECSVDDIDHL
                     GNRRVRSVGELIENQYRIGLVRMERAIKEKMSLQDIETMMPHDLINAKPVSAVVNEFF
                     GSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHSTHYGRICPIETP
                     EGPNIGLIVSLSTYARVNEFGFIESPYRLVENGRVLDEVKYMTAIEEENQLISPADRP
                     LDKKGKFEEELISVRKGGDFVSATPEEIRMMDVAPDQLVSVAAALIPFLEHDDANRAL
                     MGSNMQRQAVPLMCPEVPVVGTGMESVVARDSGAVMVAKRSGVVESVDASRIVVRADK
                     PEEDGLDTGVDIYTMSKYQRSNQDTCFNQKPIISIGDRVRKRDILADGPATDNGELAL
                     GRNVMVAFMSWGGYNYEDSILVSERIVKEDIYTSIHIEEFETMARDTKLGKEEITRDI
                     PNVGEEALRNLDESGIIRLGVSVKPGDILVGKITPKGETQLSAEEKLLRAIFGEKASD
                     VRDTSLRVPPGVEGTVIDAKIFTRKGAEKDLRSQAIEEEAIEKLTKDRDDEIRILAES
                     IKKDIARLVVGKTAAGRIVDVKKKTLLKKGEKITRELLQEIPFMYWKDVTLAEHDGSE
                     EKLANLLSVMERKIDFVKARFEEKLEKIKASDELPPGVIKMVKVYVAIKRKLSVGDKM
                     AGRHGNKGVLSRILPEEDMPYFSDGSTVDIILNPLGVPSRMNVGQILETHLGWAAKSI
                     GEGLNELLEKDHHLQQIKSELRKTYSSPDFDEFLEKASAEELKRFVGRLKKGMLVASP
                     VFDGCPEPQIRKLLDTAGLPENGQAVLFDGRTGEPFDQEVTVGIIYMLKLHHLVDNKI
                     HARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWAMEAYGASYTLQEFLTVKSDDVAGR
                     TRIYESIVKGEHTFEPGLPESFNVLVKELQSLGLDVELVEEE"
     assembly_gap    3982..3991
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3993..8042
                     /gene="rpoC"
                     /locus_tag="PLR47_03970"
     CDS             3993..8042
                     /gene="rpoC"
                     /locus_tag="PLR47_03970"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOG12128.1"
                     /translation="MKISIASPEKILSWSNGEVKKPETINYRTFKPERDGLFCAKIFG
                     PVKDYECLCGRYKRMKHRGVVCEKCGVEVIQSKVRRERMGHITLATPVAHIWFLKSLP
                     SRIGNVMDLTLKELEKVLYFESWIVLDPKETPLKKKELLTDEEFIEAKEQYGETGFVA
                     GIGAEAVRQLLEEIDLEKLYEELRAEVVTSLSDTKKKKIVKRLKVVEALRKSGNKPEW
                     MVLTVLPVIPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLQELNAPEIIIRN
                     EKRMLQEAVDVLFDNGRRGRAITGTNKRPLRSLSDMLKGKQGRFRQNLLGKRVDYSGR
                     SVITVGPDLRLHQCGLPKKMALELFKPFVYNRLLEKGYVTTVKSAKKMVERETSEVWD
                     AVDEVVREYPVMLNRAPTLHRLGIQAFEPVLIEGKAIRLHPLVCTAFNADFDGDQMAV
                     HVPLSIEAQTEARVLMMSTNNILSPANGKPIIIPSQDIVLGIYYLTRAKKDVKGEGMI
                     FSGPDEVRSALDAGAIDLHARIKVRIESELKETTAGRIILYEILPAEIGFDAINKLMN
                     KKELANLIDHCYRVCSDKTTVLLADRIKDLGFKYATISGLSFAVGNMIIPENKKNIVA
                     QADKDVLKIQKQYMDGLITDGERYNKVIDIWAQATEKIASEMLTGISTEEVAGSDGKK
                     RRIESFNPIYMMADSGARGSGQQLRQLAGMRGLMAKPSGEIIETPITANFREGLTVLQ
                     YFISTHGARKGLADTALKTANSGYLTRRLVDVAQGCIITEQDCETVDGLYVSALMEGG
                     EIIETAGERVLGRVALQEIRDPFTEEVIVKANEAIDETLAEKIDRAGIERVNIRSVLT
                     CRAKHGVCAMCYGRDLAHGHLVNIGEAVGIVAAQSIGEPGTQLTMRTFHIGGTAKFDE
                     HSTLEARHDGRVKFVSLNTAKTKENDYVVMNRHGEIHVLDEQNRSRGKYTVPYGAHIK
                     VGDESFVKRGQLIAEWDPFSIPILAEVDGIIKYGDIIEGKTMQEQVDEVTGLSRKVIV
                     EFKSGDLRPRVSIKDSKGKTAKIVGSNATARYFLSVGVNIVVSEGDEVRAGDILAKIP
                     RETTKTKDITGGLPRVAELFEARKPKDFAVISEIDGLVAFGKDAKGKRKIVVTPEVGE
                     EKEYLIPKGKHISVQEGDHVFPGDALMDGVNNPHDLLAIKGEKELARYLVDEVQEVYR
                     LQGVKINDKHMEVIVRQMLRRVRITDPGDSNFIADEQVERYRFQEENDRVVAQGGRPA
                     IGDPLLLGITKASLSTQSFISAASFQETTRVLTEASLAGKTDYLRGLKENVIMGRLIP
                     AGTGLVMYRKLGISVEEEEAETEPQAATE"
     gene            8148..8519
                     /gene="rpsL"
                     /locus_tag="PLR47_03975"
     CDS             8148..8519
                     /gene="rpsL"
                     /locus_tag="PLR47_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOG12129.1"
                     /translation="MPTISQLVRKGRAKIKRKTDAPALAGSPQRRGVCVRVYTTTPKK
                     PNSALRKVARVRLTSGYEVSSYIPGIGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGTL
                     DAVGVADRKQSRSKYGAKKPS"
     gene            8536..9006
                     /gene="rpsG"
                     /locus_tag="PLR47_03980"
     CDS             8536..9006
                     /gene="rpsG"
                     /locus_tag="PLR47_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOG12130.1"
                     /translation="MPRRREIEKREILPDPKYSNKLVAKFVNSLLKRGKKSVAESILY
                     GAFDIIEKRVKEQPVEFFEKAVNNVKPMIEVKSRRVGGSTYQVPTEVVPARRTALAIR
                     WLISNAQERTEKTMREKLAAELIDAANNRGGAIKKRESVHKMAEANKAFAHYRF"
     gene            9064..>9371
                     /locus_tag="PLR47_03985"
     CDS             9064..>9371
                     /locus_tag="PLR47_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOG12131.1"
                     /translation="MAKKKFERTKPHLNIGTIGHVDHGKTTLTAAITKYLAKKGFAEF
                     RAFDSIDNAPEEKERGVTINISHVEYETEKRHYAHVDCPGHADYIKNMISGAAHMDGT
                     "
BASE COUNT         2320 a         2588 c         2791 g         1662 t
ORIGIN      
        1 tgtcagctac aagatcggcg atgtccggta cgacatgaag gagtgcattc aaaagggcat
       61 gacctatgcc gctcctttga aaatcgtggt ccgtctggtg gtgtttgaca ccgaccggct
      121 cgccgacggc cgcatcagcc gggaaacgtc cgagaccaag ccgatccggg acatcaagga
      181 gcaggaaatt tacttcggtg aaatcccgct gatgacggaa aacggcacgt tcatcgtcaa
      241 cggctccgag cgcgtgatcg tcagccagct ccaccgctcg cccggcatct ttttcgacca
      301 tgacaagggc aagaccgtcg cctccggcaa actgatttat tcggcgcgcg ttattcccat
      361 ccgcggatcc tggctggatc tggaattcga ttccaaggac ctgctgtatg tccgcatcga
      421 ccgccgccgc aaaatgcccg tgacgattct gctcaaagcc atgggcaact cgacggaatt
      481 tatcctgaac tatttctacc agattgaaaa gatcaccctc gagggggaaa agatttattt
      541 cggcgtcgat gaatccctgg ccgggcagaa ggcgccggaa gacatcaagg acccgaaaag
      601 cgacgaagtc atcgtcaaga aggggcgcaa gctcaccaag ctcctgctca aaaagatcat
      661 ggacgccggg atcaagcgcg tggcgatcaa ggaagcggac ctgaccggga aaattctttc
      721 ttcggacgtc cgggaccccg gcacggggga aatccttttc cgctgcaacg aagagcttcc
      781 cgtcaacggc cttgaaatcg cccaggagaa gggcgtcagg gaactgacct tcatccatat
      841 cgatgaagac ctggacaacg cctccattcg cgacacgctg ctcatggacc gcatcgacag
      901 cgaggaagag gcgatcatgg agatctaccg caggcttcgc cccagcaatc cgcccacccc
      961 ggaaaccgcg acgaagtttt tcaaaagcct cttttttgaa ctggaaacct acgatttgtc
     1021 ggatgtcggc cgcgccaaga tgaactacaa actgaggctg aacgtgccga cggatttgac
     1081 ggtgctgcgc aacgaagaca ttctggcttc cgtgaaatat ctgatcgatt tgaaaaacgg
     1141 cgtgggcgag tgcagcgtgg acgatatcga tcacctgggg aaccgccgcg ttcgctccgt
     1201 gggcgaactg atcgaaaacc agtaccgcat cggtctggtg cggatggaac gggcgatcaa
     1261 ggaaaaaatg agcctgcagg acatcgaaac catgatgccc catgacctga tcaacgccaa
     1321 gccggtgtcg gccgtggtga atgaattttt cggctccagc cagctttccc agttcatgga
     1381 ccagaccaat ccgctgtcgg aaatcacgca caagcgccgc ctgtcggcgc tggggcccgg
     1441 ggggctcacc cgcgagcgcg ccgggtttga agtccgcgac gttcattcca cgcactacgg
     1501 ccgcatttgt ccgatcgaga cgccggaagg ccccaacatc gggctgattg tgtcgctttc
     1561 cacctacgcc cgcgtcaacg aattcggctt catcgaaagt ccgtaccgcc tggtcgagaa
     1621 cggccgggtg ctcgatgaag tgaaatacat gacggccatc gaagaggaaa accagctcat
     1681 ctccccggcg gaccggccgc tcgacaagaa ggggaaattt gaggaagaac tgatttcggt
     1741 ccgcaaaggc ggcgactttg tttcggccac gccggaagag atccggatga tggacgtagc
     1801 ccccgatcag cttgtttccg tggcggcggc cctgattccg tttctggagc atgacgacgc
     1861 caaccgcgcg ctcatggggt ccaacatgca gcgccaggcc gtgccgctca tgtgccccga
     1921 agtgccggtg gtcggcacgg gcatggaatc ggtcgtggcc cgcgattccg gcgccgtcat
     1981 ggtggccaaa cggtccggcg ttgtcgaaag cgtggacgct tcgcgcatcg tggtgcgcgc
     2041 cgacaagcct gaagaagacg gcctggacac cggggtggac atttacacga tgtccaagta
     2101 tcagcgatcc aatcaggaca cctgctttaa ccagaagccc attatttcca tcggcgaccg
     2161 cgtccggaag agagacattc tggccgacgg tcccgccacc gacaacggcg aactggcgct
     2221 gggccggaac gtgatggtgg ccttcatgtc ctggggaggg tacaactacg aggactcgat
     2281 tctggtcagc gaaagaatcg tcaaagaaga tatttatacc tcgatccata ttgaagaatt
     2341 tgaaaccatg gcccgcgaca ccaagctggg caaggaagag atcacgcgcg acattcccaa
     2401 cgtcggagag gaggcgctcc gcaacctcga cgagagcggc atcatccggc tgggcgtgtc
     2461 cgtcaagccc ggcgacatcc tggtcggcaa gatcacgccc aagggggaga cgcagttgtc
     2521 cgccgaagaa aaacttcttc gggcgatttt cggcgaaaaa gccagcgatg tgcgcgatac
     2581 gtcgctccgc gtgccgcccg gcgtggaagg cacggtcatc gacgccaaga ttttcacgcg
     2641 caagggcgcc gaaaaggatc tgcgctcgca ggcgatcgaa gaagaggcga tcgaaaagct
     2701 gacgaaagac cgcgacgacg aaatccgaat cctggcggaa tccatcaaga aggacatcgc
     2761 caggctggtg gtcggcaaaa cggcggccgg caggatcgtg gacgtgaaaa agaaaaccct
     2821 tctcaaaaaa ggcgaaaaaa ttacccggga actcttgcag gagattcctt tcatgtactg
     2881 gaaggacgtc acgctggcgg agcacgacgg ttcggaggaa aaactggcga acctgctttc
     2941 cgtgatggaa agaaaaatcg atttcgtgaa ggcccgtttt gaggaaaagc tggagaagat
     3001 caaggccagc gatgaactgc ctcccggcgt cattaaaatg gtgaaagttt atgtcgccat
     3061 taaaaggaaa ctgtccgtcg gcgacaaaat ggcgggccgc cacggcaaca agggcgtgtt
     3121 gtccaggatt cttccggaag aggatatgcc ctatttttcg gacggctcca cggtcgatat
     3181 tatcctcaat ccgctgggcg ttccctcccg aatgaacgtc ggccagatcc tcgaaaccca
     3241 tttgggctgg gcggccaaat ccatcggcga gggcctgaat gaactgctgg aaaaggacca
     3301 ccatctccag cagatcaaaa gcgaattgag aaaaacctac tcttctccgg attttgacga
     3361 gtttctggaa aaggcgtccg ccgaagagct caagcggttt gtcggccgcc tgaaaaaagg
     3421 catgctggtg gccagccccg ttttcgacgg ctgtcccgaa ccgcaaatcc gcaagctgct
     3481 agacacggcg gggcttccgg agaatggaca ggcggtcctt ttcgacggcc ggaccggaga
     3541 gccttttgat caggaagtca cggtcggcat catctacatg ctgaagctgc accacctggt
     3601 ggacaataaa attcacgccc gctccatcgg gccctattcg ctggtgaccc agcagccgct
     3661 gggcggcaag gcgcagttcg gcggtcagcg gctgggagaa atggaagtat gggccatgga
     3721 ggcctacggg gcttcctaca ccctgcagga atttttgacg gtcaagtccg acgacgttgc
     3781 cggacgaacg cggatttacg aatccatcgt caaaggggag cataccttcg agccgggcct
     3841 gccggaatcc ttcaacgtgc tggtgaagga gctgcagagc ctcgggctgg acgtggaact
     3901 ggtcgaggaa gagtagcccc gctaagatgt tttaaaaaac aggagaaaaa tctgtggaag
     3961 acgttttcag ctattttgaa annnnnnnnn ncatgaagat ttcgatcgcc tcgccggaaa
     4021 agatcctttc ctggtccaac ggcgaagtca agaagccgga gacgatcaat taccggacct
     4081 tcaagccgga gcgcgacggc ctgttctgcg ccaagatttt cgggccggtc aaggactatg
     4141 agtgtctttg cgggcgctac aagcggatga agcaccgggg cgtggtctgc gaaaaatgcg
     4201 gcgtcgaagt cattcagtcc aaggtgcgcc gcgagcggat gggccacatt accctggcca
     4261 cgccggtcgc gcacatctgg tttttgaaaa gtctgcccag ccgcatcggc aacgtgatgg
     4321 atctgacgct caaggagctg gaaaaagtgc tctactttga gtcctggatt gtcctggacc
     4381 cgaaagagac gccgctgaaa aagaaggagc ttctcaccga cgaggagttt atcgaagcca
     4441 aggagcagta cggggaaacc ggatttgtcg ccggcatcgg cgcggaagcc gttcgtcagc
     4501 ttctggaaga aatcgacctg gaaaaactct acgaagaatt gcgggcggaa gtcgtcacgt
     4561 cgctttccga caccaaaaag aaaaaaatcg tcaaaaggct caaagtcgtc gaagccctgc
     4621 gcaagtccgg caacaagccg gagtggatgg tgctgaccgt cctgccggtc attccgccgg
     4681 atttgcggcc cctggttccg ctggacggcg gccgtttcgc cacgtccgac ctcaacgacc
     4741 tgtaccggcg cgtaatcaac cggaacaacc ggctcaaaag gctccaggag ctcaacgcgc
     4801 cggaaatcat tatccgcaat gaaaagcgca tgctgcagga agccgtggac gttctgtttg
     4861 acaacggccg ccgcggacgc gcgatcaccg ggaccaacaa gcgcccgctg cgctctctct
     4921 cggatatgct caaaggcaag caggggcgct tccgccagaa cctgctgggc aagcgcgtgg
     4981 actactccgg gcgttccgtc atcacggtgg gccccgacct gcggctgcat cagtgcgggc
     5041 tgcccaaaaa gatggcgctc gaactcttca agccgttcgt ctataaccgc cttctggaaa
     5101 aaggctacgt gacgacggtc aaaagcgcca agaagatggt cgaacgggaa acctccgagg
     5161 tctgggacgc cgtggacgaa gtcgtgcggg aataccccgt gatgctcaac cgcgcgccca
     5221 cgctgcaccg cctgggcatc caggcgttcg agccggtgct gatcgaaggc aaggcgatcc
     5281 gcctgcatcc gctggtctgc acggccttca acgcggactt tgacggcgac cagatggccg
     5341 tccacgtgcc gctgtccatc gaggcgcaga ccgaagcgcg cgtgctgatg atgtccacga
     5401 acaacatcct ctccccggcc aacggcaagc cgatcatcat tcccagccag gacatcgttc
     5461 tggggattta ctatctgacc cgggcgaaaa aggacgtgaa gggcgagggc atgatttttt
     5521 cgggcccgga cgaagtgcgc tccgcgctgg acgcgggggc gattgacctg cacgcgcgga
     5581 tcaaggtccg catcgagtcg gagctgaagg aaacgacggc cggccgcatc atcctctacg
     5641 agattcttcc cgcggagatc ggttttgacg cgatcaacaa gctgatgaac aagaaggaac
     5701 tggccaacct gatcgaccac tgctaccggg tctgcagcga taaaaccacg gtgctgctgg
     5761 ccgaccgcat caaggatctg ggattcaagt acgcgacgat ttccggcctg tcgttcgccg
     5821 tcgggaacat gatcattccc gagaacaaga aaaacatcgt ggcgcaggcc gacaaggatg
     5881 tgctgaaaat ccagaagcag tacatggacg gtctgatcac ggacggcgaa cggtacaaca
     5941 aggtgatcga catctgggcg caggcgaccg aaaagatcgc ctccgaaatg ctgaccggca
     6001 tcagcacgga agaagtggcg ggttccgacg gcaaaaagcg cagaatcgag agcttcaacc
     6061 cgatttacat gatggcggat tccggcgccc gcgggtccgg acagcagctt cggcagctgg
     6121 ccggcatgcg cggcctgatg gcgaagccct ccggcgagat catcgaaacg ccgatcacgg
     6181 ccaatttccg tgaaggcctg acggttctgc agtactttat ttccacgcac ggcgcccgca
     6241 agggtctggc ggatacggcc ctgaaaacgg cgaactccgg ctacctgacc cgaagactgg
     6301 tcgatgtggc tcagggctgc atcatcaccg agcaggattg cgaaacggtt gacggactgt
     6361 atgtgtccgc gctcatggaa ggcggtgaaa tcattgaaac cgccggcgag cgtgtgctgg
     6421 gccgcgtcgc cctgcaggaa atccgggacc ccttcaccga agaagtgatc gtcaaggcca
     6481 atgaggccat cgatgaaacc ctggccgaaa aaatcgaccg agcgggcatt gagagagtca
     6541 acatccggtc cgttttgacc tgccgcgcca aacacggcgt ctgcgccatg tgctacgggc
     6601 gcgacctggc ccacggccat ctggtgaaca tcggcgaggc ggtcggcatc gtagccgccc
     6661 agtccatcgg cgaacccggc acgcagctca ccatgagaac gttccacatc ggcggcacgg
     6721 ccaagtttga cgagcactcc accctggagg cgcgtcatga cggccgggtc aaattcgtga
     6781 gcctgaatac ggccaagacg aaagaaaacg attacgtggt catgaaccgc cacggcgaaa
     6841 tccatgtgct ggacgaacag aaccggagcc gcggcaaata taccgttccc tacggcgccc
     6901 acatcaaagt gggagacgaa agctttgtga agcgcgggca gctgatcgcc gagtgggacc
     6961 ccttctccat accgattctg gccgaagtgg atggaattat taaatacggc gatattattg
     7021 aaggaaaaac catgcaggaa caggtcgatg aggttaccgg cctctccaga aaggtgattg
     7081 tcgagtttaa gagcggcgat ttgcggcccc gcgtgtccat caaggacagc aaggggaaga
     7141 ctgccaagat cgtcgggtcg aacgcgacgg cccgctattt cctgtcggtg ggcgtcaata
     7201 tcgtcgtctc cgaaggcgat gaggttcgcg cgggcgacat tctggcgaag attcccaggg
     7261 aaacaacaaa aaccaaggat atcacaggcg gtctgccgag ggtcgcggag ctctttgaag
     7321 ccagaaagcc taaggatttc gccgtgatca gcgaaatcga cggtcttgtc gccttcggca
     7381 aggatgccaa gggcaagcga aaaattgtcg tgacgcctga agtcggcgag gaaaaggaat
     7441 acctgattcc caagggcaag cacatcagcg tccaggaggg cgaccacgtg ttccccggcg
     7501 acgctttgat ggacggcgtc aacaatcccc atgaccttct ggccatcaag ggcgaaaaag
     7561 aactggcccg ttacctggtg gatgaagttc aggaagttta tcgtttgcag ggcgtcaaga
     7621 ttaatgataa acacatggaa gttatcgtcc gccaaatgct gcgccgcgtc cggatcaccg
     7681 atcccggcga ctccaatttc attgccgacg aacaggtaga gcgctatcgt ttccaggagg
     7741 aaaacgaccg ggttgtcgct cagggcggcc gtcccgccat cggcgatccg ctgcttctgg
     7801 gaatcaccaa agcgtcgctt tccacgcaga gcttcatatc ggcggcttcc ttccaggaaa
     7861 ccacccgggt gctgacggag gcttcgctgg cgggcaagac ggactacctg aggggcctca
     7921 aggagaacgt cattatgggg cgtctgattc cggccggaac cggcctggtc atgtaccgca
     7981 agctgggcat cagcgtcgag gaagaggagg cggaaacgga gccgcaggcg gcgacggaat
     8041 aaacgaaaaa aaagcataaa agagcttgac agaaacagct tgaattgata gttaccgcag
     8101 atttgctact cccgtcaaaa acggcgggac agcgttcggg agggaagatg ccgacaatta
     8161 gtcaattggt tcgaaaaggt agagccaaaa tcaagagaaa aacggacgcg ccggcgctgg
     8221 ccggttcgcc gcagcggcgc ggggtttgcg tgagagtgta cacgaccacg cccaaaaagc
     8281 cgaattcggc gctgcgcaag gtggcaaggg tccggctgac cagcgggtat gaagtatcat
     8341 cctacattcc cggcatcggc cacaacctgc aggagcactc tgtagtgctg gttcgcggcg
     8401 gcagagtgaa ggatttgccc ggcgtccggt accatatcgt ccggggtacg ctggatgccg
     8461 tcggggttgc cgaccgcaag cagagccgat caaaatacgg agcgaaaaag cccagttaga
     8521 tgaaggattg aaaaaatgcc acgaagacgg gagattgaaa aaagggagat tctgccggat
     8581 cccaaataca gcaataaact ggttgccaag tttgtgaatt cattgctcaa acgtggtaaa
     8641 aagagcgtcg cggaaagcat tctgtacggc gcttttgaca ttattgaaaa gcgcgtcaag
     8701 gaacagccgg tggaattctt cgaaaaagcg gtcaacaacg tcaagccgat gattgaagtg
     8761 aagtcaaggc gcgtaggcgg gtccacctat caggtgccga cggaagtcgt tcccgcccgg
     8821 cgcacggctc tggcgatccg ctggctcatt tccaacgcgc aggagcgcac cgaaaaaacc
     8881 atgcgggaaa agctggcggc ggagctgatt gacgcggcca ataaccgggg cggcgccatc
     8941 aagaaaagag aatccgtgca taaaatggcg gaggccaaca aagcgttcgc gcattataga
     9001 ttttaaataa acttataaaa cgacaaggaa aacaaccatt caaagttaag gaggaaaggt
     9061 gagatggcaa agaaaaaatt tgaaaggacc aagccgcatt taaatatagg gacgatcggt
     9121 cacgtggatc acggcaagac gacgctgacg gccgccatta ccaaatacct ggcgaaaaaa
     9181 ggcttcgcgg aattccgtgc gtttgattcc atcgacaatg cgcctgaaga aaaagagcgc
     9241 ggcgtcacga tcaatatttc ccatgtggaa tacgagacgg aaaaaaggca ttacgcccac
     9301 gtggactgcc ccggccacgc cgactatatc aaaaacatga tttccggcgc cgcccacatg
     9361 gatggaacga t
//
LOCUS       DAOSIM010000057         9129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_7211_length_9129_cov_7.956910, whole genome shotgun sequence.
ACCESSION   DAOSIM010000057 DAOSIM010000000
VERSION     DAOSIM010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9129
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7211_length_9129_cov_7.956910"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3513)
                     /locus_tag="PLR47_03990"
     CDS             complement(<1..3513)
                     /locus_tag="PLR47_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG12132.1"
                     /translation="MNFVKKSLKYPQVTLSILALTFLVGVYSLMAMPRREDAKIQIRV
                     GQVIALYPGANALQVEEQVTRKLEQHLFQYAEVRKEKTTSVTSDGMAVITVWLNNDVK
                     NFDTFWSKLNHQLNVLKALSLPQGVYGPIVNSEFGDTEALMIAIEMDRPQYAQLREYA
                     QKLEDTFRTMREVSKIKRIGEQKEQIVVYTDSAKLALYGVRLDQVMKILLSQNTIGPA
                     GGLKTSDVRVPLYTQGYYKTEAQIGDQIVGFAKTGEVVRLRDVARLQRQYEEPSSKIF
                     VNGKSSMLLAVQMHEGYNIVAFGKAVDRKLAEASQWLPAGVKLTTIVNQPKIVERNVS
                     HFLFEFVLAIAAVILVIILLLPFRVAAVAATAIPMTVAMTFALLHFFGIELHQVSLSA
                     LIVVLGMVVDDAVVVADNYVELLDNGVPPKTAAWKSATELVVPILTATLTIIAAFLPM
                     VLLSGMVRDFIIALPLTVSIALASSFFVAMILTPILCLSFIKKGLHDQERDLSSGRKK
                     MNLLNLMQSGYNRAIVWSMAHSRLTILVCLGAMGMAVVLYQILPQKFFPEAERNQLIV
                     DLWMPTGTKLEKTEQAIGGIQDLVQRDPDVVSLATFVGASIPRFYYNFTQGNPASNFA
                     QILVNTRLDEDAKRLRHELNLRADEAVPEGRLQARLLQQGVPSNASVEIRIIGDDIAT
                     LKEIGDRVMDILRQTRAVAFLRLDSREDYYGVAVRLKDGAERLGFTTESVAKSIYAGF
                     TGAPVSTLYEGNVAVDIVLRLDEKSRRHFGDVENLYLTSPVTGAGVPLRQIASLEPQW
                     LSGRINHLNGLRTLTVQCEPADGYLASQVLKMAGQRIADLSLPAGYKIEYGGEYENQK
                     ETFSEMLTALVVSLLLIFLVLLFQFRNLKETLIVMLTVPLSMFGAMLGLLMTGNPFGF
                     TAFVGLISLSGIVVRNAIILLDHADELRRGGASVATAALESGKRRLRPIFLTASAAAI
                     GVIPMIISGSPLWSPLASVIAVGIMLSMVTSLFLVPVLYACLMKPVDQDRKTPAGLQA
                     EGALSVGRKLMILVLASVCLLSFPSGAQEPPEALNLQKVIDLAAQNNRLLTIKQLQVQ
                     EKQQKVYEDRVKFFPSLIVGGAYQYNSNIGELSLKRGMLGQVAVGPISVPLPSADTTL
                     DLGHADTYTAGVLMYQPISQIPKIM"
     gene            complement(3510..4553)
                     /locus_tag="PLR47_03995"
     CDS             complement(3510..4553)
                     /locus_tag="PLR47_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG12133.1"
                     /translation="MKMKMSGFFFLWPVLFLMFGCGDREAARPEAAPVFVIAKKIEKQ
                     AAARVIAVSGNIEGSKTARLGFMVAGKINYVAAREGEIIGKGQLLASLDPENYRIAKE
                     IADANVAQIQDEYSRLNLMHERGSISESDYSKISNGLKMARAQQRLHTKNLAETRLYS
                     PFRGILLKRGVEVGEIIGAGLPLFGVSDIRTVHVNASVPETDLRFIKKGVKAKIYVAS
                     IDAAFSGRVIEVGALAEPATRAFSVRMEVQNPGEILRPGMTAEIQIPSGQKEDVVKIP
                     GDAVLRDMDNTAYVFVADDVKHKAFRRKISLGKIDEDGIEVVAGLAPGELVVVGGQHK
                     LQNGSLIALKQVL"
     gene            complement(4582..5205)
                     /locus_tag="PLR47_04000"
     CDS             complement(4582..5205)
                     /locus_tag="PLR47_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG12134.1"
                     /translation="MSEKEKKILDAAIAVISEKGFSAASTSEIARQAGVAEGTIFRYF
                     KTKKDILRGILIHLGNVIAELLVVYGLKKILYQDGREDLRAGIKLVLKDRMAMIDAVF
                     PMLRIVITEAMYHEDIRQILYDKIISRSVEVFEEYHREMVEKGLMKREIDAVVAGRGI
                     LGNLVLFFVYRNLFGPMIQLDDYDRELDQVIDAIMYGIAIPAVGDKK"
     gene            5432..6310
                     /gene="prmC"
                     /locus_tag="PLR47_04005"
     CDS             5432..6310
                     /gene="prmC"
                     /locus_tag="PLR47_04005"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOG12135.1"
                     /translation="MRLAAKGKMTVREIIFEATQTLEKAGIPSARLDAEVLLAFLLRC
                     NRLAFIKNPDEAPSKKQLADFERLIARRANGEPVAYLTGRKAFWSFVLEVNPSVLIPR
                     PDTEVIVEEALSVAQREAWSNPRILDIGTGSGAIALALACEIPGAAITATDISSAALK
                     TAQKNAAALKLDQPVVFREGDLFEPVDGRFDLIVSNPPYIGAEDFESLPAGVKNFEPD
                     EALRAGQTGLEFYQKLIYQAHGYLAENGWLLLEIGATQEQEVRAIFEAHKDFYERVDV
                     REDYAGLPRVIKGRRR"
     gene            6311..7558
                     /gene="murA"
                     /locus_tag="PLR47_04010"
     CDS             6311..7558
                     /gene="murA"
                     /locus_tag="PLR47_04010"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOG12136.1"
                     /translation="MDKIVISGGRPLHGDVRISGAKNAALPVLTAALLTAEPCTFSNI
                     PDLVDIKTACKLLRNMGVEIEGAGTVKISAEKITDSVAPYDLVKTMRASILVLGPLVA
                     RTGKARVSLPGGCAIGARPVNLHIKALQDMGATVDLKEGYIEAKAGRLKGADIYFDLP
                     TVTGTENIMMAATLADGVTVLNNAAREPEIVNLAEVLQGMGANISGAGTDVITITGVS
                     ELSGTEAAVIPDRIEAGTFMIAAGITGGEINVLGCVPRHLEALINKLRDTGMRIRPTE
                     GGLRVAAGSKISSVDVKTLPHPGFPTDLQAQIMAYLCVASGLSVITETVFENRFMHVS
                     ELMRMGADITIQGGSAVVRGVPSLNGAQTMATDLRASASLILAALVAKGTTEISRVYH
                     IDRGYENIEKKFSALGADIKRVK"
     gene            7562..8869
                     /gene="hisD"
                     /locus_tag="PLR47_04015"
     CDS             7562..8869
                     /gene="hisD"
                     /locus_tag="PLR47_04015"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOG12137.1"
                     /translation="MKILRADAKAFHNFFAALRTRGGAFSPELLADVARIVQEVSARG
                     DKALFSYTRKFDGHDLTAATVEATDEEKKEALARVKPRDLEVIRLSAERIEKYHRRQI
                     AQGWSMKDEDGVELGQRILPLARAGIYAPGGKAFYPSTILMAAIPAHLAGVKEILLAS
                     PVKDGRLSPLIAAAAEAARVDRIFKIGGAQAVAALAYGTESVPRVDKIVGPGNAYVAA
                     AKKLVFGQVAIDMIAGPSEVAIIADQTADPVFAAADMLAQAEHDEKAAAVLFTPDPDL
                     AREVEGEIRRQMKTLSRKKIIQKSLSSFGAIIITADIDEAAALTNLFAPEHLELLVEN
                     PTNVLRQIRSAGSVFLGPYTPEALGDYIAGANHILPTEGTARFSSPLGVYDFYKRMSI
                     LSFPQAAFEKLAPQTRHFAEMEGLDAHANSVQVRCKSAKGSIA"
     gene            complement(8916..>9129)
                     /locus_tag="PLR47_04020"
     CDS             complement(8916..>9129)
                     /locus_tag="PLR47_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12138.1"
                     /translation="RCAAQECEEHHIITKACHIDQLMDEVMKFANLQNKRRPVVELIK
                     AEMNKDIVYAIDHEDPPIIASGRFYV"
BASE COUNT         1944 a         2650 c         2623 g         1912 t
ORIGIN      
        1 catgattttc ggaatctgcg aaatgggctg atacatcagc actcccgccg tgtacgtgtc
       61 cgcgtggccc aggtccagcg tcgtgtcggc cgacggcagc ggaacactga tcgggccgac
      121 ggcgacctgg ccaagcattc ccctcttcag cgacagctca ccgatgttgc tgttgtactg
      181 ataggcgccg cccacgatca gcgacggaaa gaatttaacc cggtcttcgt acaccttctg
      241 ctgtttttcc tgcacctgaa gctgcttgat ggttaaaagc cggttgtttt gcgcggccag
      301 gtcgatcacc ttttgcaggt tcaaagcttc gggaggctcc tgcgctccgg aaggaaaaga
      361 cagcaggcaa acggaagcca ggacaaggat catcaacttc ctgcccacgc tcaaagcacc
      421 ttctgcttgc agcccggccg gcgttttccg gtcctgatcg accggcttca tcaggcaggc
      481 gtacagcacc ggcactaaaa acagggacgt caccatggaa agcatgattc ccaccgcgat
      541 cacgctggcg agcgggctcc acagcggtga gccggaaatg atcatgggaa tcacgccgat
      601 ggccgccgcg ctggccgtca ggaagatggg gcgcagtcgc ctttttccgg actcgagcgc
      661 ggctgtcgcg acggacgcgc cgccgcggcg cagttcatcg gcgtggtcca gcaggatgat
      721 cgcgtttctc accacgatgc cggagaggct gatcaggccg acaaacgcgg taaatccgaa
      781 cgggttgccg gtcatcagca gccccagcat ggccccgaac atgcttagcg gaacggtcag
      841 catgacgatc agcgtttcct tcaaattgcg gaactggaaa agcaggacca ggaaaatcaa
      901 aaggaggctg acaaccagcg ccgtcagcat ttcggagaag gtttccttct ggttttcgta
      961 ctcgcccccg tattcgattt tgtagcccgc cggcagggaa aggtctgcga ttcgctgtcc
     1021 ggccattttc agcacctgcg aggccagata gccgtccgcc ggctcgcact gcacggtcag
     1081 cgtgcgcagt ccgttgagat ggttgatccg gccgcttagc cactgcggct ccagggaggc
     1141 gatctggcgc agcggcacac ccgctccggt cacgggagac gtcaggtaaa ggttttccac
     1201 gtcgccgaaa tgcctccggc ttttttcatc cagacgaagc acgatgtcca cggccacatt
     1261 cccttcgtag agcgtggata cgggagcgcc ggtaaatccg gcatagatgg atttggccac
     1321 actttcggtg gtaaatccca gacgttcggc gccgtccttg agccggacgg cgacgccgta
     1381 ataatcctcc cggctgtcca ggcggagaaa ggcgacggcc cgcgtctgcc ggagaatatc
     1441 catgacccgg tccccgatct ccttcagtgt cgcgatatcg tcgccgatga tccggatttc
     1501 cacggaggcg ttcgagggaa ccccctgctg gagcagacgg gcctggagcc tgccttccgg
     1561 gactgcctcg tcggcccgca ggttcaactc atggcgcagc cgctttgcgt cttcatcgag
     1621 gcgcgtattg acgagaatct gcgcgaaatt ggaagcgggg tttccctgcg tgaaattgta
     1681 gtaaaagcgg ggaatgcttg cgccgacaaa cgtcgcgagg gaaaccacgt cggggtcgcg
     1741 ttgaaccaaa tcctgaattc cgccgatcgc ctgctccgtt ttttccagct ttgttccggt
     1801 gggcatccac aaatccacga taagctggtt gcgttcggct tccggaaaaa atttctgcgg
     1861 gaggatctga tacaggacaa ccgccattcc catggccccg aggcacacca ggatggtcag
     1921 ccgtgaatgc gccatgctcc agacgatggc gcggttgtag ccggactgca tcaagttgag
     1981 cagattcatt ttcttcctgc ccgaagataa atcgcgctcc tgatcgtgaa gacctttttt
     2041 gataaaagac aggcacaaaa tcggcgttaa aatcatggcg acaaaaaagg aggacgccag
     2101 ggcaatggag acggtcagcg gcagagcaat gatgaaatct ctcaccatgc ccgacagcag
     2161 gaccatcggc aaaaaggcgg cgatgatggt gagggtcgcc gtcaggatgg gcaccaccag
     2221 ctcggtcgcg cttttccagg cggcggtttt cggcggaacg ccgttgtcta agagctccac
     2281 gtaattgtcg gcgacaacaa ccgcgtcgtc caccaccatg cccagcacca cgatcagagc
     2341 cgaaagagaa acctggtgca gttcaatgcc gaaaaaatgc agcagggcga acgtcatcgc
     2401 gaccgtcatg ggaatcgccg tggcggcgac cgcggcaaca cggaaaggaa gcaggagaat
     2461 gatgaccagg atcaccgcgg caatggccag gacgaactca aacaggaagt ggctgacatt
     2521 ccgctctacg attttgggct ggttgacgat cgtggtcagt ttgaccccgg ccggcagcca
     2581 ttgggacgct tccgcgagct tccggtccac cgcttttccg aaggcgacga tgttgtatcc
     2641 ttcgtgcatt tgaacggcaa gcagcatgct gcttttgccg ttgacgaaga ttttggacga
     2701 cggttcctcg tactgtctct gaaggcgggc cacatcccga aggcggacga cttcgccggt
     2761 tttcgcgaag ccgacgatct gatctccgat ttgggcttcc gttttgtagt atccctgcgt
     2821 gtaaagcggg acgcggacat cggacgtttt caggccgccg gccggaccga tcgtgttttg
     2881 cgagagcagg atcttcatca cctggtccag ccggaccccg tagagcgcca gcttcgcgct
     2941 gtcggtgtaa acgacaatct gctccttttg ctcgccgatg cgcttgattt tcgacacctc
     3001 tctcatcgtc cgaaaggtgt cttccagttt ttgcgcgtat tcccgaagct gggcgtattg
     3061 gggccggtcc atttcaatgg cgatcatcag cgcttccgtg tcgccgaact ccgagttcac
     3121 gatgggacca tacaccccct gcggcaggct caaggctttc aggacgttca gttgatggtt
     3181 cagcttgctc cagaaggtgt caaaattttt gacgtcattg ttcagccaga cggtgatgac
     3241 tgccatgccg tccgaggtca cggaggtcgt tttctccttt cgcacctccg cgtactggaa
     3301 cagatgctgc tccagcttcc gggtgacctg ctcttccacc tgcagggcgt tggcgccggg
     3361 atacagagcg atcacctggc ccacgcggat ctggatcttg gcgtcctcgc ggcgaggcat
     3421 tgccatcagg gaatacacgc cgaccagaaa cgtcagggcc agaatggaca gggtgacttg
     3481 cggatatttg agcgattttt ttacaaaatt cataagacct gcttgagggc gatcaaagat
     3541 ccgttctgca gcttatgttg gccgccgacc acgacaagct cgcctggcgc aagccccgcg
     3601 accacctcga tgccgtcttc atcgattttt cccagcgata ttttgcgccg gaaggccttg
     3661 tgcttgacgt cgtccgcaac aaaaacatag gccgtgttgt ccatgtcgcg caaaaccgcg
     3721 tctccgggaa tcttgacaac atcttctttt tggccggacg ggatttgaat ctcagccgtc
     3781 atgcccggcc ggagaatttc accgggattc tgcacctcca tccggacgga aaatgcccgc
     3841 gtggccggtt ctgcgagggc cccgacttca atcacccggc ccgaaaacgc cgcatcgatc
     3901 gacgcgacgt aaattttggc tttcacgccc tttttgatga agcgcagatc ggtttccggc
     3961 acagaggcgt ttacatggac ggtgcgaata tcggacacgc cgaagagcgg cagaccggcg
     4021 ccgatgatct ctcccacttc cacgccgcgc tttagcaaaa tgccgcggaa gggcgaatac
     4081 aatctggtct cggccagatt tttcgtgtgc agcctctgct gggctctggc cattttcagc
     4141 ccgttggaga ttttggaata gtcgctttcg gagatgctgc cgcgctcgtg catgaggttc
     4201 agccggctgt attcatcctg gatctgggcc acattggcat cggcaatttc cttggcaatc
     4261 cggtaatttt caggatcgag gctcgccagc agctgcccct tgccgatgat ctccccttct
     4321 ctggccgcga catagttgat cttcccggcc accatgaacc ccagccttgc ggtttttgac
     4381 ccttcaatgt tgccgctgac ggcaatgacc ctggccgccg cctgcttttc gatctttttg
     4441 gcgatcacga aaacgggggc ggcctccggc ctggccgcct cccggtcgcc gcagccgaac
     4501 atcagaaaaa gaacaggcca caaaaagaaa aatcccgaca ttttcatttt cataagaccc
     4561 tctcgatcaa aaccgcttcc tctatttttt gtcgccgacc gccggtattg cgattccgta
     4621 catgatcgcg tcaatgacct ggtccagctc ccggtcgtaa tcgtcgagct ggatcatggg
     4681 gccgaacaga ttgcggtaaa cgaaaaaaag aaccagattg cccagaatgc cgcggcccgc
     4741 gacgaccgcg tcaatttccc ttttcatcag ccctttttca accatctccc ggtgatattc
     4801 ctcaaatacc tcgacggacc gggaaatgat tttgtcgtag aggatctgcc ggatgtcttc
     4861 atgatacatc gcctccgtga tcacgatgcg cagcatggga aaaacggcat cgatcatggc
     4921 catgcggtct ttcaaaacca gcttgatgcc ggcgcgaagg tcttccctgc cgtcctgata
     4981 aagaattttt ttgaggccgt acaccaccag cagctccgcg atgacattgc ccaggtgaat
     5041 cagaatgccg cgcagaatgt cctttttggt tttaaagtag cggaaaatgg ttccttccgc
     5101 gactccggcc tggcgcgcga tttcgctcgt gctggccgcg ctgaagcctt tttctgaaat
     5161 gacggcaatc gccgcatcaa gaattttttt ttctttttcc gacattttca aatcaggcgc
     5221 ctgcatgatg aatacctttt ttcattaagc tcagtaatta ctaatacttg caaaaacttt
     5281 tctccgttgc cgttatgttg agtgagtaat cactcatttt tgtgtttttg tcaatatttt
     5341 tttgagcgaa gttccgttcc ataaaaaatg gccgtttgga tattcggtcc aaataagata
     5401 tagattgaaa aatcatcggg gcggccgtgt aatgcgcctc gccgcaaagg gaaaaatgac
     5461 ggtccgggaa atcatttttg aagcgacgca aacgctggag aaagccggga ttccttccgc
     5521 tcgccttgac gctgaagttt tgctggcttt tcttttgcgg tgcaaccgcc tggcgttcat
     5581 caaaaatccg gacgaggcgc caagcaaaaa acaattggcc gactttgagc ggctgatcgc
     5641 cagacgggcg aacggagagc ctgtggctta ccttacgggc cgaaaagctt tctggtcctt
     5701 tgtcctggaa gtaaatcctt ccgtgctgat tccccgcccc gatacggaag taattgtcga
     5761 agaggcgctt tccgttgcgc agcgggaagc ctggagcaat ccgcgcattc tggacatcgg
     5821 cacgggaagc ggcgcaatcg cgctggcgct ggcctgcgaa atccccggcg cggccattac
     5881 cgccacggac atctcgtcgg cggccctcaa aacggcgcaa aaaaatgccg ccgccctgaa
     5941 actggatcag ccggtcgtgt ttcgtgaggg cgatcttttc gagccggttg acggccggtt
     6001 tgacctgatt gtttccaatc ctccctacat cggagcggag gatttcgagt ccctgcccgc
     6061 cggggtgaaa aacttcgagc cggatgaagc gctccgggcg ggccaaacag gtctggaatt
     6121 ttaccaaaag ttgatatatc aggcacacgg atacctcgcg gaaaatggct ggcttttgct
     6181 tgaaatcggc gctacacaag agcaggaagt tcgcgccatc tttgaggccc ataaggattt
     6241 ttacgaacgg gtcgatgtgc gtgaagacta cgcgggattg ccgcgcgtga tcaaaggaag
     6301 gagaaggtaa gtggataaaa ttgtgatttc cgggggcagg ccgcttcacg gagacgttcg
     6361 aatcagcggc gcgaaaaatg ccgccctgcc ggtgctgacc gccgcgcttc tgaccgcaga
     6421 gccctgcacg ttttccaata ttccggacct ggtggacatc aaaacggcct gcaagctctt
     6481 aagaaatatg ggcgtggaaa ttgaaggcgc gggcacggta aaaatcagcg ccgaaaaaat
     6541 caccgacagc gtcgccccgt atgatctggt caaaacgatg cgcgcgtcca tcctggttct
     6601 agggccgctg gtggcccgca cgggcaaagc acgcgtttct ctgcccggcg gctgcgccat
     6661 cggagcgcgc cccgtcaatc tccatatcaa ggcgctccag gacatgggcg cgaccgtgga
     6721 cctgaaggaa ggctatatcg aggccaaagc cggcaggctc aaaggcgccg atatttattt
     6781 tgatctgccc acggtgaccg gcacggaaaa tatcatgatg gcggcaaccc tggcggacgg
     6841 cgtcaccgtg ctcaacaacg ccgcgcggga gccggaaatc gtcaacctgg cggaggtgct
     6901 gcagggcatg ggcgccaaca tcagcggcgc gggaacggac gtgatcacca tcaccggcgt
     6961 atccgaactt tccggaaccg aggcggccgt catccccgac cgcatcgaag ccggcacgtt
     7021 catgatcgcc gccggcatca ccggcgggga aatcaacgtg ctgggatgcg tcccccggca
     7081 cctggaagcg ctgatcaaca aactgcggga caccggcatg agaatccggc ccactgaagg
     7141 agggcttcgc gtcgccgccg gaagcaaaat ctccagcgtg gacgtcaaaa cccttcccca
     7201 tcccggcttt ccgacggatc tgcaggcgca gatcatggcc tacctgtgtg tcgcaagcgg
     7261 cctgtcggtc atcacggaga cggtttttga aaaccgcttc atgcacgtga gcgaactgat
     7321 gcgcatgggc gcggacatca cgattcaggg cggcagcgcc gtggtgcgcg gcgttccctc
     7381 tctcaacggc gcgcagacca tggccacgga cctgcgggcc tccgcctcgc tgattctggc
     7441 ggcgctggtc gcaaaaggca cgacggaaat ttcgcgggtc tatcacatcg accgcggtta
     7501 cgagaacatc gaaaagaaat tttccgcgct gggcgcggac atcaaaaggg taaaataaaa
     7561 gatgaaaatt cttcgagccg acgcgaaagc cttccacaat ttttttgcgg ccctgcggac
     7621 aaggggcggc gcgttttcgc cggagctgct ggcggacgtg gcccgcatcg ttcaggaggt
     7681 gtccgcgcgg ggggacaaag ccctgttttc ctacacccgg aaattcgacg gccacgacct
     7741 gactgcggca accgtcgaag caacggatga ggaaaagaaa gaagcgctgg cccgcgtaaa
     7801 accgcgggac ctggaggtca tccggctttc ggccgaacga attgaaaagt atcaccgccg
     7861 gcagatcgcc cagggatggt cgatgaagga cgaagacggc gtcgaactgg gccagcggat
     7921 cctgccgctt gcgcgggcgg gcatttacgc gccgggcgga aaggcctttt atccttccac
     7981 gatcctgatg gcggcgatcc ccgcgcatct cgcgggcgtc aaagaaattc tgctggcaag
     8041 ccccgtaaag gacggccggc tttccccgct cattgccgcg gcggccgaag ccgcccgggt
     8101 ggaccgcatc ttcaagatcg gcggcgcgca ggccgtcgcc gccctggcgt acgggaccga
     8161 aagcgttccg cgcgtggata aaatcgtggg gccgggcaac gcctatgtcg ccgcggccaa
     8221 gaagctggtg ttcggacagg tggccatcga catgatcgcc ggtcccagcg aggtggcgat
     8281 catcgccgat caaaccgccg accccgtttt cgcggcggcg gacatgctgg cgcaggctga
     8341 acatgacgaa aaggcggcgg ccgtgctgtt cacgcccgat cccgatctgg cgcgcgaggt
     8401 tgaaggagaa atcagacggc agatgaaaac cctgtcccgg aaaaaaatca ttcaaaaatc
     8461 cctgtcttcc ttcggcgcga tcatcatcac ggctgatatc gacgaggcgg cggcgctgac
     8521 caatctcttc gcgccggagc acctggagct tctggtggag aatccgacga atgttctcag
     8581 gcagatccgc agcgccggct ccgtgttcct ggggccctat acgccggaag ccctcggcga
     8641 ttacatcgcc ggcgccaacc acattctgcc cacggaaggc acggcgcggt tctcttcgcc
     8701 cctgggcgtt tatgatttct acaagcggat gagcattctg tctttcccgc aggcggcttt
     8761 cgaaaagctt gccccgcaga cacggcactt cgctgaaatg gaaggactgg acgcgcacgc
     8821 caattccgtg caggttcgat gtaaaagcgc taaaggatca atagcgtagg gaaaggtttt
     8881 aaaccgttcc ctacgctatt gaaatgctta ttggattaaa cgtagaatcg accggacgcg
     8941 atgatcggcg gatcctcatg atcgatcgcg taaacgatgt ctttgttcat ttccgccttg
     9001 atcagttcaa cgaccgggcg tcttttgttc tgaagattgg caaatttcat gacctcgtcc
     9061 attaactgat cgatatggca ggccttggtg atgatgtggt gctcctcgca ttcctgcgcg
     9121 gcgcagcgc
//
LOCUS       DAOSIM010000058         9109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_7231_length_9109_cov_7.231610, whole genome shotgun sequence.
ACCESSION   DAOSIM010000058 DAOSIM010000000
VERSION     DAOSIM010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9109
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7231_length_9109_cov_7.231610"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..518)
                     /locus_tag="PLR47_04025"
     CDS             complement(72..518)
                     /locus_tag="PLR47_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOG12139.1"
                     /translation="MDIQAKLNEDMVTAAKAKDKIRLSALRMLKTALHNREIDLMRPL
                     NETETMQVLSGLVKQRKDSIEQFAKGGRQDLVEKEEAELKILQDFMPAQMSDEEVETL
                     IQKAIADVGAASVKDMGKVMKVLMPQITGRADGKAAGEKVKALLSR"
     gene            complement(535..876)
                     /locus_tag="PLR47_04030"
     CDS             complement(535..876)
                     /locus_tag="PLR47_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOG12140.1"
                     /translation="MAECIFCKIIKGDIPSRKVYEDDAVLAFDDISPMAPAHVVIIPK
                     KHIATLMDVDAKADITGKLIAAAQQVAEIKQVAESGFRLAINCNADGGQVVFHLHMHL
                     LGGRKLEDQLG"
     gene            complement(898..1629)
                     /gene="hisA"
                     /locus_tag="PLR47_04035"
     CDS             complement(898..1629)
                     /gene="hisA"
                     /locus_tag="PLR47_04035"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOG12141.1"
                     /translation="MLIIPAVDVKNGQCVRLAQGDFNRVTVYAENPADVASRWAQKGA
                     KRIHLVDLDGSVAGLPKNAPVIVEIAQSVPVPVEVGGGIRSMETVNYYLENGVSSVIL
                     GTAAIQDESFVRTAARTHRGKIILGIDALGGEVAVRGWTQKTGENAVELARRYARDGI
                     QAIVYTDIARDGMETGVNVEQTRALAKAVDIPVIASGGVATLADIDALLAVKDCSFYG
                     VIVGRALYTGAIALEDAIAKTQRAS"
     gene            complement(1708..2394)
                     /locus_tag="PLR47_04040"
     CDS             complement(1708..2394)
                     /locus_tag="PLR47_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12142.1"
                     /translation="MILHQVHSKSIVCAMWLLGLLILCPGVPQAREAAKADDKTVARV
                     AVIPFQKVLPEETSSTVQCPICGSVNSSGVIFEGAEKIVEEMATDHLRDYKGVEILPA
                     EKAAGVYRRVSTSLLKQSLLQVFLETGRELKADVLMAGFVYRCRERVGYDYSAERPAA
                     VAFEIHLISVSDGRTLWRGTFDKTQKSLMEDVFQVSSFFRGGGKWLTARQLTKLGVDQ
                     IFKTFPGFER"
     gene            complement(2414..3001)
                     /gene="hisB"
                     /locus_tag="PLR47_04045"
     CDS             complement(2414..3001)
                     /gene="hisB"
                     /locus_tag="PLR47_04045"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOG12143.1"
                     /translation="MARKAKIVRKTTETDIALDIDLDKSAASRIDTTIPFLNHMLELF
                     ARHGQFKLVVKSKGDTEIDDHHLVEDLGICLGQAVGQALGKKTGINRYGSASVPMDES
                     LCRVDMDICGRPYLIYKVRYERRKIGGFDPALVKEFFKAFTDHSGITLHITLEYGSNS
                     HHIIESVFKAFARALRQAVTINPEISGVLSTKGTL"
     gene            complement(3016..3888)
                     /gene="hisG"
                     /locus_tag="PLR47_04050"
     CDS             complement(3016..3888)
                     /gene="hisG"
                     /locus_tag="PLR47_04050"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOG12144.1"
                     /translation="MNVLKLGIPKGSLEKNTVDLFQKAGWRITYDSRSYFPDIDDPEL
                     SCSLVRAQEMSRYIEDGHLDLGLTGIDWIMENDSDVVAVQDLIYSKVSTRQARWVLVV
                     RDDSPIRSLADLEGKHISTELVNFTRRYFAEKKIKVGVEFSWGATEAKVVDGLVDAVV
                     EVTETGSTIRANKLRIVHELMKTNTQLIANREAWNDPWKRKKIDQIRTLLTGSLLAMG
                     KVGIKLNVSRANLDRVMSLLPSLKAPTISSLYSEEWFAIESVIDTGVVRDLIPQLLEA
                     GAQGIIEYPLNKVI"
     gene            complement(3885..4259)
                     /gene="hisI"
                     /locus_tag="PLR47_04055"
     CDS             complement(3885..4259)
                     /gene="hisI"
                     /locus_tag="PLR47_04055"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOG12145.1"
                     /translation="MIEPNFNKTDGLIPAIVQDAQTKDILMLAYMNRASWEATLQSGK
                     ATFWSRSRQKLWLKGETSGHVQIVKEIYIDCDEDTLLLQVEQLGGAACHTGHRSCFFR
                     KREGGDFVVVGEKVFDPGEVYK"
     gene            4454..4750
                     /locus_tag="PLR47_04060"
     CDS             4454..4750
                     /locus_tag="PLR47_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12146.1"
                     /translation="MSLLLGGVVAAVIGLISLIFWWSDFLAILRGALPIFLFLGGALA
                     VYVGFDDIQDKIREERQRQNEELERAREEIESAKAQAERYRDELDRLKDEKKQS"
     gene            5033..6064
                     /locus_tag="PLR47_04065"
     CDS             5033..6064
                     /locus_tag="PLR47_04065"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOG12147.1"
                     /translation="MKKNSLLTVISIICAAVFFVFGWTCGVQAQTTLTYSIFFPATHA
                     QAKAGEAWAKEIEKRTGDKVKINIFAGGSLTPADQCFDGVVKGISDIGMSCFAYTRGR
                     LPLLEALDLPMGYPSGMVATRVANDFAKKMKPKELDNVKVLYLHAHGPGLLHTQKPVR
                     TMADVKGMKIRSTGLSAKIVEMLGGVSVAMPQGGTYEALQKGVVQGTLAPMETLKGWK
                     QAQVVKYTTDCTNIGYTTAMFVVMNRNKWNALPKDVQKVFDDVSEEWIAVAGKAWDDA
                     DIEGYKFSAELGNKVIKLSAAEQARWKKAVKPVAEGYIKSAESKGLPGRQAVAEVELL
                     IKKHAKAKK"
     gene            6077..6562
                     /locus_tag="PLR47_04070"
     CDS             6077..6562
                     /locus_tag="PLR47_04070"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOG12148.1"
                     /translation="MFSPAKYLTRAGKAFNILACAAVIAMMLLSTADVFLRLLGKPIP
                     GTYELVGFLGTIVVSFALAFTSMEKGHIAVEILVEKLPQRAQLAIESFCNLIGALLFG
                     VIACQAVLYALDIRKSGEVSLTLQMPPYPFIFGIAAGCALLCLLLVADFIKSVRRTID
                     A"
     gene            6559..7860
                     /locus_tag="PLR47_04075"
     CDS             6559..7860
                     /locus_tag="PLR47_04075"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOG12149.1"
                     /translation="MTPTTTGLIGFAVLIILMFLRMPVGFVMALVGYLGFGVLVSWSA
                     ASSLVIRDFYSTFSSYSLTVIPLFVLMGQIAFHSGISGRLFNAAHKFFGHLPGGLAIA
                     TIGACAGFSAVCGSTSATAATMASVALPEMKKYKYDPALATGVVAAGGSLGILIPPST
                     IFIIYGIMTEQSIGELFMAGVVPGILLSCFFVLSVLIWAYFRPEIGPRGPKSTWTEKF
                     KSLSGLIETLLLFVLVMGGLFAGFFTPTEAAGIGAAGTILIALIGRHLTWKGFKNALY
                     DTTRITCMIFVVVAGATVFGHFLAVTRIPADIGTFVSGLELPPMVIMGIIIVIYLLLG
                     CLMDSLAMIMLTIPIFYPVVLTLGFDPIWFGVIIVVVSGMGVITPPVGINAYVVAGVA
                     RDVPLHVIFKGTLHMLYAMIALAVLLMVFPKIATFLPQMLK"
     gene            complement(8021..>9109)
                     /locus_tag="PLR47_04080"
     CDS             complement(8021..>9109)
                     /locus_tag="PLR47_04080"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF013019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HOG12150.1"
                     /translation="RVTYFTGAFPSACGKTSTAMVPGQQIVGDDIAYIRHGEDGRAYA
                     VNIEQGIFGIIEDVNPIDDPVIYEALTTPRELIFSNVLVKDDKPYWLGMGRILPEEGH
                     NFSGEWKKGKKDEKGNEILLAHKNARYTIRLNELKNVDPKLYDPEGVPVSGIIYGGRD
                     SDTSVPVYQSFDWAHGMFIGASLESETTAATIGAVGVRELSPMANLDFLVVPLGTYLS
                     NHLKFGNRLIVKPLVFATNYFLKEDGKFLNEKVDKKVWLMWMEGRVHGEYKAIETPIG
                     YIPHYEDLAKLFVQIFGKKFSREMYDKLFAVRVEKFLEKLVRVEKAYRDEENIPDEFH
                     AILDMLRNRLTAAKAKAGQSVITPGEFE"
BASE COUNT         1886 a         2670 c         2413 g         2140 t
ORIGIN      
        1 tgtcgtcaaa gcgcacgaag gatcgcctgc cctgttttga tggtttgagt ccgtttcgcg
       61 aataacctgc cttaccgcga cagaagcgct tttacctttt caccggcggc tttcccgtcc
      121 gccctgccgg taatctgcgg catcagcacc ttcatcacct tgcccatgtc cttgacggaa
      181 gcggcgccca cgtcggcaat cgccttttgg atgagcgttt ccacttcttc atccgacatt
      241 tgcgcgggca tgaaatcctg cagaattttc agctccgctt cttctttctc cacaagatcc
      301 tgccggcccc ccttggcaaa ctgttcgatg gaatcttttc tctgcttgac gagcccggaa
      361 aggacctgca tcgtttccgt ctcgttgagc ggccgcatca ggtcgatttc cctgttgtgg
      421 agcgccgttt tcagcatgcg aagcgcggag agccggattt tgtctttggc cttcgcagcc
      481 gtcaccatgt cttcattcaa tttggcctga atatccataa gcactttcct cgttttaccc
      541 caactgatct tccagttttc tgcctcccag cagatgcatg tgcagatgaa ataccacctg
      601 ccccccgtcg gcgttgcaat tgatcgccag ccggaatccg ctctcggcca cctgctttat
      661 ttcagctacc tgctgcgcgg ccgcaatcaa ttttcccgtg atgtccgctt tcgcatcaac
      721 atccatcagc gtcgcaatgt gttttttggg aatgatgacg acgtgcgccg gcgccatggg
      781 actgatatcg tcaaacgcca gcacagcatc gtcttcgtaa actttcctgg aaggaatgtc
      841 tcccttgatg atcttgcaga atatgcactc tgccatatgc ttttcctcct tccggcttta
      901 tgaagcccgc tgtgtcttcg caatcgcgtc ttccagggcg atggcgcccg tgtaaagcgc
      961 gcggccgaca atgacgccgt aaaacgaaca gtccttgacg gccagcaggg catcgatgtc
     1021 ggccagcgtc gccacgcccc ccgacgcgat caccggaata tcgacggctt tcgccagagc
     1081 cctggtttgc tccacattga cgcccgtctc catgccgtcg cgagcaatgt ccgtatagac
     1141 aatggcctgg atcccgtcgc gggcatagcg ccgcgccagt tcgaccgcgt tctcgcccgt
     1201 cttttgcgtc cagccgcgga ccgcgacctc ccctccgagc gcgtcgatcc ccaggatgat
     1261 ttttccccga tgggttcggg ccgccgtccg gacgaacgat tcgtcctgaa tggccgccgt
     1321 ccccaggatc acgctcgata cgccgttttc caggtagtag ttgaccgttt ccatgctgcg
     1381 gatgccgccg ccgacctcaa ccggcaccgg caccgactgc gcgatttcga cgatgaccgg
     1441 cgcgttcttc ggcaatccgg cgacggagcc gtccagatcc acgaggtgaa ttcttttggc
     1501 ccctttttga gcccagcgac tggccacgtc cgcgggattt tcagcgtaaa cggtcacccg
     1561 gttgaaatcg ccctgcgcca gccgcacgca ttgtccgttt ttaacatcga ccgccggaat
     1621 aatcagcacc ttgagtgact ccttgaagga tccgtttcat gaaacatcgg ccggagcatt
     1681 tccgcgggca ttcacgcccc ggtccggtca gcgctcaaaa ccgggaaagg ttttaaaaat
     1741 ctggtcgact cccagcttcg tcaactggcg cgccgtcagc cacttcccgc cgcccctgaa
     1801 gaaagaagat acctgaaaaa catcttccat cagagatttt tgcgttttgt cgaaagttcc
     1861 ccgccacagg gtgcggccgt cgctcacgga aatcagatga atttcaaagg caaccgccgc
     1921 ggggcgctcc gcggaataat cgtagcccac tctttcccgg cagcggtaca caaaccccgc
     1981 catcaggaca tccgctttca gttcccgtcc ggtttccagg aaaacctgca acaaagactg
     2041 cttgagaaga ctcgtcgata cccgccggta aacgcctgcc gccttttccg cgggaaggat
     2101 ctccacgccc ttataatccc tcaaatgatc ggtcgccatt tcctcaacga ttttttccgc
     2161 gccttcgaag atgacgccgc tggaattgac gctgccgcaa atcgggcatt gaacggtgga
     2221 ggacgtctct tccggcagca ccttctgaaa agggatgacc gcgacgcgcg ccacggtttt
     2281 atcgtcggcc ttcgccgctt cccgcgcctg cgggacgccg gggcaaagaa tgagcaaccc
     2341 cagaagccac atggcgcaga cgatgctttt gctatgaacc tggtgcagga tcacgagacg
     2401 cctgatcttt ctttcaaagc gttcccttgg tggacagaac gcctgaaatt tccggattga
     2461 tagtcaccgc ctgcctgagc gcccgggcaa aggctttgaa gacggactcg atgatgtgat
     2521 gactgttgct gccgtattcc agggtaatgt gcagggtaat gccgctgtga tccgtgaacg
     2581 ccttgaaaaa ttccttgacc agcgcgggat caaacccgcc tattttcctg cgttcatacc
     2641 ggaccttgta aatcagatag gggcggccgc agatgtccat gtccacgcgg cacaggctct
     2701 cgtccatcgg cacggacgcc gatccgtagc gattgatgcc cgtctttttg cccagcgcct
     2761 gccccacggc ctgccccagg caaatcccca ggtcttccac caaatgatga tcgtcgattt
     2821 ccgtgtcgcc cttgcttttt acaaccagtt tgaactgccc gtgacgggcg aacagctcca
     2881 gcatgtgatt caggaaggga atggttgtat cgatcctgct ggcggcggat ttatccaaat
     2941 caatgtccag cgcgatatcc gtttccgttg tcttacggac gatctttgcc tttctggcca
     3001 tggctgccct ccggattaga tgactttatt caggggatac tcgatgatgc cctgcgctcc
     3061 cgcctccaga agctggggaa tcagatcccg cacgaccccc gtatcgatga cggattcaat
     3121 ggcgaaccat tcctcggaat agaggctgga aatggtcggg gctttcagcg acggcagaag
     3181 gctcatcacg cggtccagat tggctctgga aacattcaac ttgattccaa ccttgcccat
     3241 cgccagcagc gacccggtca gcagcgtccg gatctgatcg attttctttc tcttccaggg
     3301 atcgttccag gcctcgcggt tggcgatgag ctgcgtgttg gttttcatca gttcatgcac
     3361 gatcctgagc ttgttggccc ggatggtcga tcccgtttcc gtgacttcca cgacggcgtc
     3421 caccagaccg tcaaccactt tcgcctcggt cgcgccccag gaaaactcca cgccgacctt
     3481 gatcttcttt tcagcaaaat atctccgggt aaagttgacc agctccgtcg agatgtgttt
     3541 gccctccaaa tccgcaagcg aacggatggg cgaatcgtcc cgcaccacca gcacccacct
     3601 ggcctggcgc gtggaaacct tggaataaat cagatcctgc acggccacta cgtcggagtc
     3661 gttttccatg atccagtcaa tgccggtcag ccccaggtcc agatgaccgt cttcgatgta
     3721 gcgggacatt tcctgcgccc tgaccagcga gcaggaaagc tccggatcgt caatatccgg
     3781 gaaatagctg cggctgtcat aggtgatccg ccagccggct ttttgaaata aatcaaccgt
     3841 attcttttcc aggctccctt tcgggattcc caacttcaaa acattcattt atatacttct
     3901 ccgggatcaa aaaccttctc gccgacgacc acgaaatcgc cgccctcgcg tttgcggaaa
     3961 aaacaggaac gatgccccgt gtggcaggcc gccccgccga gctgctccac ctgaagcaaa
     4021 agggtgtcct cgtcgcaatc gatataaatc tctttgacaa tctggacgtg gccggaggtt
     4081 tcccccttca gccacagctt ttgccgggaa cgactccaga acgtggcctt gcccgactgc
     4141 agcgtcgcct cccaggaggc cctgttcatg taggccagca tgaggatgtc tttggtttgg
     4201 gcatcctgca cgatggccgg aatcaacccg tccgttttat taaagtttgg ttcgatcatg
     4261 ggtcccttcc gcaaattcaa ttatttaccg ccttataaaa ataagatgtt taatggatga
     4321 accccattaa atcaagagaa attttagctt tcgtttcgtt gatttccggc ttgcctgcgt
     4381 cttccaatca tgataaaaaa accgtcagca tcgtattaaa aggatttaac tttattggcg
     4441 gaggattggg cttatgtctt tgctgctcgg aggcgtcgtt gccgcggtca tcggactgat
     4501 cagtttgatt ttctggtggt ctgatttcct ggccattctc cggggtgcgc tgcccatctt
     4561 cctttttctg ggaggcgctc tggccgtcta tgtcggtttt gacgatattc aggacaaaat
     4621 acgggaagaa agacagcggc aaaatgaaga attggagcga gccagggaag aaattgaatc
     4681 cgccaaagcc caggccgaac gctaccgcga tgaactggac cggttaaagg atgaaaagaa
     4741 gcaaagctga aacacggcgg cgcaccgcac aaatacatct tgacaccgcc ggtaaaatca
     4801 cataagagca acattcaata catcgttatg gacttaacat gagtgcattt tttacagtaa
     4861 atccgttctc gccttattat acctacatgt attgatgcct gcttgcccga ttccttcagg
     4921 taagcaggct tttccaaaag caccagcgca aatccaaaac cagcatcaac caaagaacgc
     4981 ggatatgtat ctattgatgg aaacattggg gatatcggag gaaacgggtt atatgaaaaa
     5041 gaatagctta ttgacagtca tttcaatcat ctgtgcggca gtgttttttg tttttgggtg
     5101 gacttgcggc gttcaggcgc agacaaccct gacatacagc atcttttttc cggccacgca
     5161 tgcgcaggca aaagccggcg aagcctgggc aaaggaaatt gaaaaacgca ccggggacaa
     5221 ggtcaaaatc aacatcttcg ccggcggttc gctgaccccg gccgatcagt gttttgacgg
     5281 cgtcgtcaag ggaatttccg acatcggcat gtcctgcttc gcctatacgc ggggccgcct
     5341 gccgcttctg gaagcactcg atctgccgat gggctacccc agcggcatgg ttgccacccg
     5401 cgtggccaat gacttcgcga aaaagatgaa gcccaaagaa ctggacaacg tcaaggttct
     5461 gtatctgcac gcacacggcc cggggcttct gcatacacag aagcccgtcc ggacgatggc
     5521 ggatgtcaaa ggcatgaaaa tcaggtccac ggggctttcc gctaaaatcg tggagatgct
     5581 gggcggcgtc tccgtggcca tgccgcaggg cggaacctat gaagcgctgc aaaaaggcgt
     5641 tgtgcagggc acactggccc ccatggaaac cctgaaggga tggaagcagg cgcaggtcgt
     5701 caaatacacg actgattgca ccaacattgg ctacaccacc gccatgttcg tcgtcatgaa
     5761 cagaaataaa tggaatgctc tgcccaaaga cgtgcagaag gtgtttgacg acgtcagtga
     5821 ggagtggatt gccgtggccg gcaaggcctg ggacgatgcg gatattgaag gatataaatt
     5881 ctccgccgaa ctgggcaaca aagtaatcaa actgtccgcg gcggagcagg cccgatggaa
     5941 gaaggccgtc aagccggtcg ccgaaggcta catcaaaagc gccgagagca aaggtttgcc
     6001 cggacggcag gccgtcgccg aagtggagct cctgatcaag aaacacgcaa aggcaaaaaa
     6061 ataaaaggac cgattcatgt tcagtccggc aaaatacctg acgcgcgcgg gcaaagcgtt
     6121 caacattctg gcctgcgcgg ccgtcatcgc catgatgctg ctgtccacgg cggatgtttt
     6181 tttgcgcctg ttgggcaagc ccatccccgg aacctatgaa ctggtgggat tcctgggaac
     6241 catcgtcgta tccttcgccc tggctttcac ctccatggaa aaagggcaca tcgccgtgga
     6301 gatcctggtg gaaaaattgc cgcagcgcgc gcagttggcc atcgaaagct tctgcaacct
     6361 catcggcgcg ctgctttttg gcgtcatcgc ctgccaggcg gttctctacg cgctggatat
     6421 ccggaaaagc ggcgaagtat ccctgacgct gcagatgccg ccctatccct tcattttcgg
     6481 catcgccgcg ggctgcgctc ttttatgcct gctcttggtc gccgatttca tcaaatccgt
     6541 gcggaggacg attgacgcat gactcccacc acgaccggtt tgatcggctt tgccgtactg
     6601 attattttga tgtttttgcg gatgcccgtg ggatttgtca tggccctggt cggctacctg
     6661 ggattcggcg tgctggtttc ctggagcgcc gcctcgagcc tggtcattcg cgacttctat
     6721 tccaccttca gctcctatag cttaaccgtc attcccctgt ttgtgctgat gggtcaaatc
     6781 gcctttcatt cgggcatcag cggccgcctc ttcaacgcgg cccacaagtt tttcggtcat
     6841 ctgccggggg ggctcgccat cgccaccatc ggcgcctgtg ccggcttctc ggccgtgtgc
     6901 ggctcgacca gcgccacggc cgccaccatg gcttccgtgg cgcttcccga gatgaaaaaa
     6961 tacaagtacg acccggcgct ggccaccggc gtggtcgcgg ccggcggaag cctgggaatc
     7021 ctcatacctc ccagcaccat tttcatcatt tacgggatta tgaccgagca gtctatcggc
     7081 gagctgttca tggccggcgt ggtcccgggc attctcctgt cctgcttctt tgttctgtcc
     7141 gttttgatct gggcatattt ccgtccggaa atcgggccgc gcggtcccaa aagcacctgg
     7201 accgaaaaat tcaaatcctt atccggcctc atcgaaaccc tgcttctatt tgttctggtc
     7261 atgggcggcc tgtttgccgg tttctttacg ccaacggagg ccgccggcat cggggctgcg
     7321 ggaacgattc tgatcgcttt gatcggacgc catttgacct ggaaggggtt caagaacgct
     7381 ctgtacgata ccacccggat cacctgcatg atcttcgtcg tggtcgccgg tgcgacggtc
     7441 ttcggacatt ttctggcggt gacaaggatc ccggcggaca tcggaacatt cgtttccggt
     7501 ctagagcttc ctcccatggt gatcatgggc atcatcatcg tcatctacct gcttctgggc
     7561 tgtctgatgg attcgctggc catgatcatg ctgacgattc ccatttttta tccggtcgta
     7621 ctcacgctgg gttttgaccc gatctggttc ggcgtgatca tcgtcgtcgt ttcggggatg
     7681 ggcgtcatca cgccgcccgt cggcatcaat gcctacgtgg tggcgggcgt ggcccgcgat
     7741 gtgcctttgc atgtcatctt taaaggcacg ctgcacatgc tctacgcgat gatcgcgctg
     7801 gcggtcttgc tgatggtctt tccgaaaatc gcaaccttcc ttccgcagat gcttaaatag
     7861 gctgaagact gaaggctgaa gggtaaaggc tgaaagctgc agacggaagg gaaaagattc
     7921 agagatttca ggtttaccgt gcaacagctg tcatgagatg caaaacacaa aaaaagcccc
     7981 accggaaagg acggggcttt tttgttttac cggcttccgt ttattcgaat tcgccgggcg
     8041 tgatgacgga ctgtccggct ttcgctttgg cggcggtcag acggttgcgc agcatgtcga
     8101 ggatggcgtg gaattcatcg ggaatgtttt cctcgtcccg gtaggccttt tccacgcgga
     8161 ccagcttctc cagaaacttt tccacccgaa cggcaaacag cttgtcgtac atttcccgcg
     8221 aaaacttttt gccgaaaatc tgaacaaaca gcttggcaag gtcttcgtaa tgcggaatat
     8281 agccgatcgg cgtttcgatc gccttgtatt cgccgtgcac ccggccttcc atccacatga
     8341 gccagacttt cttgtccact ttctcattca ggaatttgcc gtcttccttc agaaagtagt
     8401 tggtggcaaa aaccagcggc ttgacgatca ggcggttgcc gaacttcaga tggttggaaa
     8461 gatacgtccc cagcggaacc accaggaaat ccaggttggc catgggagaa agctcccgga
     8521 cgcccaccgc gccgatcgtc gcggcggtcg tctccgactc cagcgacgcg ccgataaaca
     8581 tgccgtgggc ccagtcaaag ctctggtaca cgggcacgga cgtgtcggaa tcgcgcccgc
     8641 cgtaaatgat gccgctcacg ggaacgcctt ccggatcgta gagtttgggg tccacgtttt
     8701 tcagttcgtt gaggcggatc gtgtagcggg cgttcttgtg ggccagcaga atctcgttgc
     8761 ccttttcatc cttcttgcct tttttccact cgccggaaaa attatggccc tcctccggaa
     8821 gaatccggcc catgcccagc cagtagggtt tgtcgtcttt caccaggacg ttggagaaga
     8881 taagctcgcg cggcgtcgtc agcgcttcgt aaatcaccgg gtcgtcgatg ggattgacgt
     8941 cttcgataat gccgaagatg ccctgttcga tattgaccgc atacgcccgg ccgtcctcgc
     9001 cgtggcggat gtaggcgatg tcgtcgccga caatctgctg gcccggcacc atggccgtcg
     9061 aggttttgcc gcaggcgctg ggaaacgcgc cggtaaagta ggtcacgcg
//
LOCUS       DAOSIM010000059         9090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_7245_length_9090_cov_7.511566, whole genome shotgun sequence.
ACCESSION   DAOSIM010000059 DAOSIM010000000
VERSION     DAOSIM010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9090
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7245_length_9090_cov_7.511566"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..529)
                     /gene="tadA"
                     /locus_tag="PLR47_04085"
     CDS             complement(56..529)
                     /gene="tadA"
                     /locus_tag="PLR47_04085"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOG12151.1"
                     /translation="MLTDEDCMNMALEEARKAFGQDEVPVGAVVANGDQILAVAHNAP
                     VSAGDATAHAEMIAIREACRASGNYRLPAARLYVTLEPCLMCAGAILQARLARVVFGA
                     RDPKAGAVFSLYRVLHDSRLNHKVEVTEGILAQACGEILSRFFKEKRIEAASRNA"
     gene            623..1183
                     /locus_tag="PLR47_04090"
     CDS             623..1183
                     /locus_tag="PLR47_04090"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOG12152.1"
                     /translation="MDAKKIFLLPVIVSLMGHAALIAASSMVDLRDRDKASQLFMVQI
                     APPPAAADPERDHSPTGEKVAKDSQDAKPVPSGDREDTVDIGSADVKYAAYLSGVKQK
                     IMRIWKYPEAAYQQEEEGVVVVKMSIEASGRLSQLALMTSSGFIHLDSGTLEVVRAAA
                     PFQPLPRQYDLSRLHIIASFNYRVEP"
     gene            1241..1873
                     /gene="lepB"
                     /locus_tag="PLR47_04095"
     CDS             1241..1873
                     /gene="lepB"
                     /locus_tag="PLR47_04095"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOG12153.1"
                     /translation="MKENKSKPAAAKTKIREFIEAILTALLIAAFIISFVVQAFKIPS
                     GSMIPTLLVGDHLFVNKFIYGVKLPYFRTTIIPVTDPKREDVIVFIYPEDRSKDFIKR
                     VIGVAGDKIEMKNKKLFINDRPYADPNGHYTDQAIFPAEAQPRDNFGPLVVPQDSFFV
                     MGDNRDHSLDSRFWGFVDLKDVQGKAFILYWSWNHEERNVRWNRIARLIQ"
     gene            1880..2956
                     /gene="mutY"
                     /locus_tag="PLR47_04100"
     CDS             1880..2956
                     /gene="mutY"
                     /locus_tag="PLR47_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOG12154.1"
                     /translation="MLKNPHETGGLPARRLLAWYKRCGRTLPWRETADPYRIWISEIM
                     LQQTRVDTVIPYYERFLKQFPDVFALARAPLQDVLKAWENLGYYSRARHLHEAAKIIT
                     GRFGGRIPNTLEAIRTLPGIGDYTAGAILSIAFERAVPAVDGNVRRILSRVFAVAKPI
                     NDPGEHRHFFELAASLVPKKRASGFNQALMDLGATVCRAKNPDCGACPLASLCLAHHR
                     HLQDRLPVMRKAPAIPRRQSVAAVLRNDKGRLLVVQRPAEGLLASFWKLPGGFLKDHE
                     NPEAGLRRLVREELGLSIRVGKKIASVDHAYTHFRVTLHVYEGFLFNGKPAAKRCPNR
                     QWAAAADLKKLPLSKIDRMAAKLI"
     gene            complement(3031..3465)
                     /locus_tag="PLR47_04105"
     CDS             complement(3031..3465)
                     /locus_tag="PLR47_04105"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOG12155.1"
                     /translation="MRRDMLEMIESNRLCVLATVSESGPHCSLMSYAASEDGTEFYMA
                     TDKNTKKYRNLAANPSVSLLIDSRADSAEGRGAATRALTVTGTVRRNLDEPGKKAALE
                     ALCRRHPDLKDFLAGSSVEVIAVKINSLQLLDGIADSHFEEV"
     gene            3608..5686
                     /locus_tag="PLR47_04110"
     CDS             3608..5686
                     /locus_tag="PLR47_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOG12156.1"
                     /translation="MKKNLITLNEFEGASLLRQSGIPVIDGIPARTEEEAAAAAEKLG
                     FPVALKICSEAVVHKTERGGVALNIQDTAALDGAVREMKNKFADIPFAVLVQKMAPSG
                     AELILGCRRDPVFGPIVLAGIGGIFTEILRDTVIDLAPVDEKAALAMLRRLKGAPLLE
                     GYRSREPLDIAAAAKALSALSCLAAERADLLEIDVNPFIVYPRGAVAVDALVRLDAGP
                     AAVPRKRTSPATVAPFFQPSSMAVIGASRSPGKGGNIILRNLQKAGYRGALYPINPTV
                     KEILGLPSYARVSDVPKPVETAMVVIPKTAVPEALADCAAKGVTNIILSTGGYSDTGE
                     EGAKEQKIIIDMARKAGIRVMGPNSIGTINPSAGMSTSIVGLDPIRPGGVSIIGQSGV
                     FSSGWARWIADTKPFGLAKVACIGNKGDINESDLLEYLTGDSATRTVGMYLEGVVEGK
                     RFIRAAAAASKTKPVVVMKAGRSEAGAAAVASHTGSLAGSDAVFDAVCRRAGLLRVQN
                     SEAFFDALAAFEKLPLPRGNRMGALSITGMGCVAATDAAEECGVDLPALQKETLKKLG
                     EVMPVWAPVRNPIDTWSAIEQHGSKKATSHIARCLMEQPDVDALLITFVLMPESMFDI
                     SEAFTEIFRRHPHKPVFASYYGGTAREIAHIHEGFAALGVPCYPTPERAVFAFGRMVE
                     YARFRGVIRK"
     gene            5785..6564
                     /locus_tag="PLR47_04115"
     CDS             5785..6564
                     /locus_tag="PLR47_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12157.1"
                     /translation="MINHGRKLFFALVVALSLTVVAEANAFELGARAYLWFPDLKKAD
                     VQTIANGIEGSDISATDMLGVGNKITYSVEAYGGFGKNHVSLMYTPFGYTTDAILATA
                     LTYNGIPYKAGDTVHTDMGYSMFDLKYQRDLINLENVLAGFSLGGIAQIKYSTGSFKL
                     NSAGKLEQDRSFNSIIPMIGVGAHIGLLANLLEARAQVTAGGYSSENYSYEALADVSL
                     TPFPFLDIHAGYRLLQQKMDVNDYKMDTLYSGPYIGLSVGF"
     gene            6786..8225
                     /locus_tag="PLR47_04120"
     CDS             6786..8225
                     /locus_tag="PLR47_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOG12158.1"
                     /translation="MERFFDPQSVVVVGASNTPFNLGSTICNMLKDYLHYDGNVYAVN
                     SKGEAVNGYPGYSSIPDLPQTPDLAVIIVAARHVPGIIRDCARKGIRHVVIESAGFSE
                     GGEIGEAMQREIDAIARQNGIRMLGPNCLGTLSSRNKFCCFYGVNPSLVEMNQIFESP
                     GSISYIIQSGGVVVLTLESLYYDLVGVNKVVSIGNKCDVDEADLIEYFQKDETEVIGI
                     YLENIADGRKLMEAARKSRKPVLVYKVGKTKEGAQAAMSHTAGMANNDRIFDAACRQA
                     GIIRLQSIDELHSLPKIFTEMPLLKGKRIAAFTNSGAFGGISADLLVQAGLQMVRLSP
                     QTQEKLKKVGQVFNIKNPVDIGPAPPQTYLDIFEILLSAEEVDGLLPLLSVWQPFVID
                     CLLELMKICKRYEKPAAIYTPNAISKSVAIRARHRLPIFETTEQAVRALSVSQTYFAS
                     LLKRNLTGDRPLWGAGLNTDHRSTGEALS"
     gene            8222..8926
                     /locus_tag="PLR47_04125"
     CDS             8222..8926
                     /locus_tag="PLR47_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOG12159.1"
                     /translation="MTGKKEKEIKDMLLKAIAGSQLALSEFESKKAVAAAGVPVTREA
                     LVQSRDEAMSKAEKIGFPVVLKGSSPELTHKTEMGMVRVNLKNKDEAAAAYDELTGKG
                     IALEGVLIQEMVKGNREFVIGLIRDPHFGPCVMFGLGGIFTEAMKDVSFRVAPLTEED
                     AREMIGEIRAAKLLDAFRGEKAVDREILVRALVGIGNLGMKHGEIAEIDINPLIVKDG
                     KPVAVDALVILQAPRA"
BASE COUNT         1958 a         2750 c         2540 g         1842 t
ORIGIN      
        1 cgtacacacg atttccagtc gtgctccttc ggcctctcag acatctctcc gcgcctcatg
       61 cattcctcga ggcggcttct atcctctttt ccttaaaaaa tctactcaaa atttcgccgc
      121 aggcctgggc gaggattccc tccgtcacct ccaccttgtg attcaggcgg ctgtcgtgca
      181 aaacacggta gagggaaaag accgcgccgg ccttcggatc gcgcgcgccg aacaccaccc
      241 gcgccagccg cgcctgcagg atcgcgcccg cgcacatcag gcagggctcg agggtcacat
      301 aaagccgcgc ggcaggcagg cggtaattgc ccgacgcccg gcaggcctcg cgaatcgcga
      361 tcatctccgc gtgcgccgtt gcatcgcccg cggaaaccgg cgcgttatgc gccacggcca
      421 gaatttggtc gccgtttgcc accacggcgc ctaccggaac ttcgtcttgc ccgaaggcct
      481 ttcgggcctc ttcgagcgcc atgttcatgc agtcttcatc cgtcagcatc ggcttcgcct
      541 tgttttaaag tcgttttttg attatcatac tgttgatttt gaggcaaatg acatatatga
      601 tcctgaacgc gaacgaaagg ccatggacgc gaaaaaaata ttccttttgc cggtgatcgt
      661 atccctgatg ggacatgccg cgctgattgc cgcgagcagc atggtggatc tgcgcgaccg
      721 ggacaaagcc tcgcagctct tcatggttca gatcgcgccg cctccggcgg cggccgatcc
      781 ggaaagggat cattccccga ccggcgaaaa agttgcaaag gacagccagg acgcgaaacc
      841 ggtcccgtcg ggagacaggg aggacacggt ggacatcggc tccgccgacg tcaaatacgc
      901 ggcctacctg tcgggcgtca agcaaaagat catgcgcatc tggaaatatc ccgaagccgc
      961 gtaccagcag gaggaggaag gagtggtggt ggttaaaatg tcgattgagg ccagcggccg
     1021 gctttcgcaa ctggccctga tgacttcctc cggcttcatc cacctcgatt ccggcacgct
     1081 ggaagttgtc cgggcggccg cgcctttcca gccgctgccc cggcagtacg acctgtcgcg
     1141 tctgcatatc atcgcctctt tcaactaccg cgtggaacca tagtagatac ctcgggaatt
     1201 cgcgcaaaac gggaaaagca cacacctctt ggggagaaac atgaaagaaa acaaatcaaa
     1261 accggcggcc gccaaaacca aaatccggga gtttatcgaa gcgatcctga ccgcgcttct
     1321 gatcgccgct tttatcattt ccttcgtcgt tcaggcgttc aagattccct ccggctcgat
     1381 gattccgacg ctgctcgtcg gcgatcacct gttcgtgaat aaattcattt acggcgtcaa
     1441 gctcccgtac ttccgcacca ccatcatccc ggtcacggat ccgaagcggg aggacgtcat
     1501 tgtgttcatt tatccggaag accgctccaa ggatttcatc aagcgcgtca tcggcgtcgc
     1561 gggcgacaaa attgaaatga aaaacaagaa acttttcatc aatgaccggc cctacgcgga
     1621 ccccaacggt cattacaccg accaggcgat ttttccggcc gaggcacagc cgcgcgacaa
     1681 tttcggcccg ctggtcgttc cccaagattc gtttttcgtg atgggcgaca accgcgatca
     1741 cagcctggac agccgcttct ggggattcgt cgatctcaag gacgtccagg gaaaagcctt
     1801 cattctctac tggtcgtgga accacgagga gcgaaacgtc cgatggaacc gcatcgcccg
     1861 gctgatacaa tagaaaacca tgttgaaaaa tccgcatgaa accggtggcc ttccggcccg
     1921 ccgcctgctc gcctggtaca aacgctgcgg gcggactctg ccctggcggg agacggccga
     1981 tccctaccgc atctggattt ccgaaatcat gctgcagcaa acccgtgtgg acacggtcat
     2041 cccctactac gagcgttttc tgaaacaatt ccccgacgtc ttcgcgctgg cccgcgcccc
     2101 cctgcaggac gtgctgaaag cctgggaaaa tctgggctac tattcccgcg cccggcatct
     2161 gcacgaggcg gcaaagatca tcaccggcag gttcggcggg cgaattccca acacgctcga
     2221 agccatccgg acgctgcccg gcatcggcga ctacacggcc ggggctatct taagcatcgc
     2281 tttcgaaagg gccgttccgg ccgtggacgg caatgtccgc cggattttga gccgcgtttt
     2341 cgccgtcgcc aaacccatca acgatcccgg ggaacacagg cattttttcg agctggccgc
     2401 atccctggtt ccgaagaaac gcgcctccgg cttcaaccag gccctgatgg acctgggggc
     2461 gacggtctgc cgcgcgaaaa atccggactg cggcgcctgc cccctggcgt cgctctgcct
     2521 ggcccatcac cgtcatctgc aggacaggct gcccgtcatg cgcaaggccc ccgcaatccc
     2581 gcgccgtcag tcagtcgccg ccgtcctcag aaacgacaaa ggacggcttc tggtggtgca
     2641 gcggccggcc gagggcctgc tggcatcgtt ttggaaactg ccgggcggtt ttttgaaaga
     2701 tcatgaaaac ccggaagccg gtctgcgccg tcttgtccgg gaagaactgg gtctttccat
     2761 ccgcgtcggg aaaaaaatcg cttccgtgga tcatgcctac acgcatttcc gcgtgacgct
     2821 tcatgtttac gagggatttc tgttcaatgg aaaaccggcg gcaaaacgtt gccccaaccg
     2881 gcaatgggcc gccgcggcgg atttgaaaaa actgcccctg tccaaaatcg accggatggc
     2941 ggcaaagctg atctgagcac cccgtcagcc ttccggcata agcggaaaac tccggccggc
     3001 gtttaccgct tcggctttcg gttttctgac tcaaacttct tcaaaatggg aatccgcaat
     3061 cccgtcgagc aattgaagcg aattgatttt caccgcaatc acttccacgg aggaaccggc
     3121 aagaaaatcc ttcaggtcgg gatggcgccg gcacagggct tccagagccg ctttttttcc
     3181 gggctcatcc aaattgcgcc gaaccgttcc cgtgacggtc agcgccctgg tagccgcccc
     3241 ccggccttcg gcgctgtctg ctctggaatc gatcagaaga ctgacggacg gattggccgc
     3301 caggttgcgg tatttttttg tgttcttgtc cgtggccatg taaaattccg tgccgtcttc
     3361 acttgcggca tacgacatca gcgagcagtg aggcccgctt tccgacacgg tggccagaac
     3421 gcacaaccgg ttggactcga tcatttccag catatcccgt cgcatctttt tcaccccttc
     3481 gacccgcaga atgatgcaga ttcaatacag gaccggccgg aatatgcaag ttgaattatt
     3541 tcaaaatcat gatagggtca ccgcgtaaaa ttcggaaata cgcatgccgc ggggaggtat
     3601 ttgcattttg aagaagaatc tgatcaccct caatgaattt gaaggagcgt ctctgctcag
     3661 gcaatcgggg attcccgtca ttgacggcat tccggcccgc acggaggagg aagcggcggc
     3721 ggcggccgaa aagctcggat ttccggttgc cttgaaaatt tgttccgagg ccgttgtgca
     3781 caaaaccgaa aggggaggcg tcgctctcaa cattcaggat actgccgccc tggacggggc
     3841 cgtgagggaa atgaaaaata aatttgccga tattcccttc gcggttctgg tgcagaagat
     3901 ggccccttcg ggcgccgaac tcattctggg gtgccgccgc gatccagttt tcggcccgat
     3961 tgttctggcg ggcatcggcg gcatttttac ggaaatcctg cgcgacacgg tcatcgacct
     4021 tgcccccgtg gatgagaagg cggcgctggc catgcttcgg cgcctcaaag gcgctcccct
     4081 gctggaaggg taccggtccc gggagcctct cgatatcgcc gccgccgcaa aagccctgtc
     4141 cgcactttcc tgtcttgccg cggagcgcgc ggatcttctt gaaatcgacg tcaacccctt
     4201 catcgtttat ccgcggggcg ccgtggccgt cgacgccctg gtgagactgg atgccggccc
     4261 cgccgccgtt ccccgcaagc gcaccagccc ggccaccgtc gcgccttttt tccagccttc
     4321 gtcgatggcc gtgatcgggg cctcgcgcag cccgggaaaa ggcggcaata tcattttgcg
     4381 aaacctgcag aaggccggct acaggggcgc gctttatccc atcaatccga ccgtcaagga
     4441 aatcctcggc cttccctcct atgcgagagt gagcgacgtg ccaaagcccg tcgaaaccgc
     4501 catggtcgtg attcccaaga cggccgtgcc cgaagcgctg gcggactgcg ccgcaaaggg
     4561 cgtgaccaat atcatcctga gcacgggcgg ctactcggac acgggagagg aaggggccaa
     4621 agaacaaaaa atcatcatcg atatggcccg caaggccggc attcgggtca tgggacccaa
     4681 cagcatcggc acgatcaacc cgtcggccgg catgtccacc tccatcgtcg gcctcgatcc
     4741 gattcgtccg ggcggtgttt ccatcatcgg ccagtccggc gttttctcgt ccgggtgggc
     4801 ccgctggatc gcggacacca agcccttcgg tctggccaaa gtggcctgca tcggcaacaa
     4861 gggagacatc aacgaaagcg acctgctgga gtacctgacc ggggattcgg ccacccgcac
     4921 cgtcggcatg tacctcgaag gcgtggtgga agggaaacgg ttcattcgcg ccgcggccgc
     4981 ggcatccaaa acaaaacccg tggtggtgat gaaagccggt cgcagcgaag ccggggccgc
     5041 cgccgtggcg tcgcataccg gatcgctggc cggatcggac gcggtatttg acgcggtctg
     5101 ccgcagagca ggtcttctgc gggtgcaaaa ctccgaagct ttttttgacg ctctcgcggc
     5161 ctttgaaaaa ctccccctgc cgcgcggaaa ccgcatgggc gcgctctcga tcaccggcat
     5221 gggctgcgtc gccgccacgg acgccgccga ggaatgcggc gtcgatctcc ccgcgctgca
     5281 gaaggagact ctcaaaaagc tgggcgaggt catgccggtt tgggcgccgg tgcgcaatcc
     5341 catcgacacc tggtccgcca tcgagcagca cgggtcaaag aaggccacca gccacatcgc
     5401 ccgctgcctg atggagcagc cggatgtcga cgcccttctg atcaccttcg tgctcatgcc
     5461 ggaaagcatg ttcgatatct ccgaagcctt caccgagatt ttccggcgcc acccgcacaa
     5521 gccggtcttt gccagctact acggcggcac ggcaagggag atcgcccata tccacgaggg
     5581 cttcgccgcg ctgggcgttc cctgctatcc tacgccggag cgcgccgtct tcgcctttgg
     5641 ccgcatggtg gaatacgccc ggttccgggg cgtcattcgg aaataagcgc cgggattccg
     5701 ggcaaatttc gccgacactt ccgattgacg aatgccggga aatatgcgag attgcaaacc
     5761 ataattcatc aaatggagga tactatgatt aaccatggaa gaaagctgtt tttcgcttta
     5821 gtggttgctt tatcgttgac ggttgttgcg gaggcgaacg cttttgaact gggcgcaaga
     5881 gcctacctgt ggtttcccga tcttaaaaaa gccgatgtcc agaccatcgc caacggcata
     5941 gagggctcgg acatcagcgc gacggacatg ctgggggtcg gaaataaaat tacgtattcc
     6001 gtagaggcat acgggggctt cggaaaaaat cacgtaagcc tgatgtacac gcctttcggt
     6061 tatacgacgg atgccattct tgcaacggct ctgacataca acggcattcc ttacaaggcc
     6121 ggcgacaccg ttcatacgga tatgggatat tccatgttcg atctcaaata ccagcgcgat
     6181 ctgatcaatc tggaaaacgt cctggcgggg ttttcactgg gaggaatcgc ccagatcaag
     6241 tattccacgg gcagctttaa gctgaactcg gccggaaaat tggaacagga ccgatccttc
     6301 aattccatca ttccgatgat cggcgtcggc gcccacatcg ggcttttggc caacctgctg
     6361 gaagcccgcg cccaggtgac cgcaggcggg tacagctctg aaaactattc ctatgaagcc
     6421 ttggcggatg tgtcccttac cccctttccg tttctcgaca tccacgcggg ctacaggctc
     6481 ctccagcaga aaatggatgt caacgattac aaaatggaca ccctctactc agggccctac
     6541 atcggcctgt cggttgggtt ctaaagacat taagacgcat cagcccggcc tgagcggcaa
     6601 agcattgccg tgcggccggg cggatgcaac aaaccattcc ggacaaaaca ccgtaagtcc
     6661 gcccaatccc ttattcctgc ccgttttcca gctgcctttt tcctgctgcc tttttcctgc
     6721 ttgacaaaag ccgccttttt gcgttgtatg gcggcgcgaa aattcgactt caggcaggta
     6781 ttcttatgga acgcttcttc gatccgcaat cggttgttgt cgttggcgcc tcaaacaccc
     6841 ccttcaatct gggctccacc atctgcaaca tgctcaaaga ttatctccat tatgacggaa
     6901 acgtctatgc cgtgaactcc aaaggagagg cggtaaacgg ctatccgggc tattcatcga
     6961 tcccggattt gccccaaacc cccgatctgg cggtcattat cgtggcggcc aggcacgttc
     7021 ccggcatcat tcgggactgc gcccgcaaag gcatccggca cgtcgtgatt gaaagcgccg
     7081 gtttttccga aggcggggaa atcggcgagg cgatgcagcg cgaaatcgat gccattgccc
     7141 ggcaaaacgg catccgcatg ctggggccca actgcctggg gaccttgagc tcgcgcaaca
     7201 agttctgctg tttttacggc gtcaacccga gcctggtcga gatgaatcaa attttcgagt
     7261 ctccgggcag catctcctac atcatccaga gcggcggcgt ggtcgtcctg acgctggagt
     7321 ctctgtacta cgacctggtc ggcgtgaaca aagtggtcag catcggcaac aagtgcgacg
     7381 tggacgaagc ggatctgatc gaatattttc aaaaggacga aacggaagtc atcggcatct
     7441 atctggaaaa tatcgcggac ggccgcaagc tcatggaggc ggcccggaaa tcccgcaagc
     7501 ccgtcctggt ttacaaagtc ggcaaaacca aagaaggcgc gcaggccgcc atgtcgcaca
     7561 cggctggcat ggccaataac gaccggatct tcgatgccgc ctgccgacag gcggggatca
     7621 tccgcctcca gtccatcgac gaactccatt cgctccccaa gatattcacc gaaatgccgc
     7681 tgctgaaagg aaagcggatc gcggcgttta ccaattccgg cgccttcggc gggatttccg
     7741 cggacctgct ggtgcaggcg ggtctgcaga tggttcgcct gtccccgcaa acccaggaga
     7801 agctgaaaaa agtcggtcag gtcttcaaca taaaaaatcc ggtggacatc ggccccgcgc
     7861 cgcctcagac gtacctcgac attttcgaga tactgctctc cgcggaggaa gtggacggcc
     7921 ttctgcccct gctcagcgtc tggcagcctt ttgtgatcga ctgccttctg gagctgatga
     7981 agatctgcaa gcgctacgaa aagcccgccg ccatctacac gccgaatgcc atctccaaat
     8041 ccgttgccat tcgggccagg caccgccttc ccatctttga gacaacggaa caggccgtgc
     8101 gggccctgtc cgtttcccaa acgtacttcg cctctctttt gaaaaggaat ttgaccggcg
     8161 acagaccttt gtggggcgcc ggcttgaaca cggatcatcg ctcaaccggg gaggccttgt
     8221 catgaccggc aaaaaagaaa aagaaataaa agacatgctg ctgaaagcta ttgcgggcag
     8281 ccagctggcc ctttcagagt ttgaatccaa gaaagctgtc gccgccgccg gcgtgcccgt
     8341 aacccgtgaa gcgctggttc aatcgcgcga cgaagccatg tcaaaagcgg agaaaatcgg
     8401 tttccccgta gtcctcaagg gatcatcacc ggagctgacg cacaagaccg agatgggaat
     8461 ggtccgcgtg aatcttaaaa acaaggacga ggcggccgcg gcctatgatg aactgacggg
     8521 aaagggaatt gccctggaag gcgttttgat ccaggaaatg gtgaagggca accgggaatt
     8581 cgtcatcggt ctgatccgcg acccgcattt cggcccctgc gtcatgttcg gcctgggcgg
     8641 catttttacg gaagccatga aggacgtcag tttccgtgtc gcgcctctca ccgaagagga
     8701 cgcccgcgaa atgatcggcg aaatccgcgc ggcaaagctt ctggatgcct tccggggtga
     8761 aaaagccgtt gaccgcgaaa tactggttcg cgctttggtg gggataggca atctgggaat
     8821 gaagcacggg gagattgccg aaatcgacat caaccccctg attgtgaagg acggcaagcc
     8881 ggtggccgtg gacgccctgg tgattctgca ggccccccgc gcctgatgaa catccatgaa
     8941 ccttgaactt tgatgcacct gtaaaaagtc ataatttacc cacattgtca tggtgaactc
     9001 gtcgaaccat gcagtagcat cgaatgctta agatcaccct tcgacgggct cagggtgaca
     9061 aatgtgactt tttacgagtt tgtcaacttt
//
LOCUS       DAOSIM010000060         8969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_7355_length_8969_cov_8.358874, whole genome shotgun sequence.
ACCESSION   DAOSIM010000060 DAOSIM010000000
VERSION     DAOSIM010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8969
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7355_length_8969_cov_8.358874"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PLR47_04130"
     CDS             <1..235
                     /locus_tag="PLR47_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12160.1"
                     /translation="LRLLRGEISARIPISLMSQYTPVPKVRSHPLLGRRVTASEYHEI
                     VEFALRLGFENLFIQEVDDRALTPDFKREHPFN"
     gene            complement(319..1287)
                     /locus_tag="PLR47_04135"
     CDS             complement(319..1287)
                     /locus_tag="PLR47_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOG12161.1"
                     /translation="MARGIESGKIFDSDADRDNFLYRLGDIFPDTATTCFAWSLMPNH
                     FHLLCRTGNIPISTVMRRLLTGYATWFNRSRKRHGHLFQNRFKSILCQEDAYLLELVR
                     YIHLNPLRAGLVQNLNKLDSYPYSGHSALMGREHRSWQETQGILMLFGNTQDAARQSY
                     RQFVGQGIEQGRRHDLTGGGLIRSAGGWEGLKRKCEEGTYQRSDERILGDGDFVSQVL
                     SKTEEKIKKSYALRNSGVDLDQVALRVSALTGVKPGDVRTAGKQPHIVNARSLFCFWA
                     VRDLGETMAGLARRLGLSIPAISKSVVRGRQIAESNGFELMQNNHN"
     gene            complement(1401..2093)
                     /locus_tag="PLR47_04140"
     CDS             complement(1401..2093)
                     /locus_tag="PLR47_04140"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOG12162.1"
                     /translation="MKAKLNKIELFFPAGVLAFCLALFWAVVFPGASTAAPPLEEVVD
                     RLQRIYEKTQDFQAGFIQETTVKSIRKTDTESGTVYFKNPGQMLWDYKKPKAKKLIIN
                     AQKAWLYLPQDKTVYVQDSGKIMKSEALIKFLSGLGKLNNDFTIQYASPAEDPDGNCL
                     LLLYPREKGASYSFLRMTVAKNNFYILQVGFDDVMGNSTLLRFSNIKMNSGLSSKLFQ
                     FQPPPGVSIFKM"
     gene            complement(2404..3474)
                     /locus_tag="PLR47_04145"
     CDS             complement(2404..3474)
                     /locus_tag="PLR47_04145"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOG12163.1"
                     /translation="MKIKIIKALPKYLKPKPDEKKLGFGQHFTDHMFTMKYREGDGWQ
                     DPVIEPYHYLQLDPTAMCLHYGQEIFEGLKAYRGANNEIYLFRPMENIRRMNVSAERL
                     CMPPMDETVVLNALRQFVVLEKEWIPREKGSSLYIRPTMIATEPALGVHPASEYLFFI
                     IAGPVGAYYAHGFGPTKIYVSDDCVRAAPGGTGHVKAAGNYAASLYASRIASTMGYTQ
                     VLWLDARENRYVEEVGTSNIFFLIEDRLITPPLGGTILPGVTRDSVIKLAAHLGVKVE
                     ESRLTITEVISAIENGTLRDAFATGTAAVISPVGQIFYHNREYLINDGKTGELAEKLY
                     NEILQIQYGTREDPFGWRMQID"
     gene            complement(3534..5063)
                     /locus_tag="PLR47_04150"
     CDS             complement(3534..5063)
                     /locus_tag="PLR47_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOG12164.1"
                     /translation="MFIKVQSMSVIGMESYPVTVEVDVSRGLPHFATVGLPDASVKES
                     RDRIKAAIKNSGYAFPRDHVTVNLAPADIRKEGTGFDLPIAVGILAAEALIEEAALDG
                     CFFIGELSLDGSIKGVRGVLPAAFKARESGIRSVFVPEENAAEAAMVEGIQVYGVRTL
                     PDVVEFLADRKIIEPLSVNADRLFQVHRHYEIDFSEIKGQKQALRALEIAAAGSHNVL
                     MIGPPGSGKSMLARRLPTILPELSFEEAIEVTKIFSVAGLLNAGETLVAARPFRAPHH
                     TISDAGLVGGGQMPHPGEISLSHLGVLFLDELPEFKRNALEALRQPLEEGAITITRSA
                     VTAAFPARFMLVAAMNPCPCGYFGDRVRRCRCSPQQIRQYQAKISGPLLDRIDLHVEV
                     PSIQYRALSGKQENESSAHVRERVVRARRRQKERFGGAGIPTNASMTEKQIRSFCPID
                     EESHQLMEMAVDKLGLSARALDRILKVSRTIADLEGAAKIEAAHVAEAIQYRSLDRNI
                     V"
     gene            5245..5730
                     /gene="moaC"
                     /locus_tag="PLR47_04155"
     CDS             5245..5730
                     /gene="moaC"
                     /locus_tag="PLR47_04155"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOG12165.1"
                     /translation="MKKLSHINDEGQVRMVDVTAKTATHRQAKARGQVTMKPATMKLL
                     AEGRIAKGNVLTAAKIAGILAAKNTGGIIPLCHPLGLTGMDITFVLDRKKSRVVIESQ
                     VRTVGQTGVEMEALTAVSVAALTIYDMCKAVDREMVIGEILLLEKSGGKSGAFVRKAA
                     K"
     gene            5745..6257
                     /locus_tag="PLR47_04160"
     CDS             5745..6257
                     /locus_tag="PLR47_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12166.1"
                     /translation="MNAVYKIRKAFRYPFVTLTVLLFVLFLTGLARGECWEKILTGAL
                     FAAALVVAVESSEREFGISENGLRIRKFFRTRHFTWAEITHLGTVILRSKAYFLLTTT
                     KGFYLFSNLLQDHTRLLGQLAEKLEDERVETEIKNYIEAPKERTSLIVLTWVALVLLV
                     AVVLTRWLAA"
     gene            6339..6641
                     /locus_tag="PLR47_04165"
     CDS             6339..6641
                     /locus_tag="PLR47_04165"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG12167.1"
                     /translation="MKAKKQINPAAGIDVQEALNKAVEEILEKKLDDLATVLSGSDNG
                     RSKLHAEVMAMVETGLFKIALRRSGNVKSSAAAFLGIHRNTFTDKMAKLGLDGEKE"
     gene            7332..>8969
                     /locus_tag="PLR47_04170"
     CDS             7332..>8969
                     /locus_tag="PLR47_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12168.1"
                     /translation="MNGPETIQSLADCVNRLKREDVQECLFFQKDRTADLTRVAGGMK
                     AFLAAEEKILEDRAAGLSSAGLETVNRRLLSLKDICWMLESDILANLQKVLSLSGAEK
                     YSSLQPAAFRKYRKLNLLLNALDRLEVRGRDSAGIQLAFFLKDAKVLEELDRRIEENG
                     WGEDYRRRTQKGDLINRSIFISGGAQNPGGGIHIAFTYKTFSVVGELGRNVADLRQWI
                     SRDAILQWFAARASADESAIAHTRWASVGSITEENCHPVNNATLRPELSDFPAYPGRE
                     AHISVVLNGDIDNYPALRRDLEQDGEQIAPDVSTDTKIIPLQIEKYLRKGQNLTDAFR
                     LAVNDFEGSHAIAMQSNLEPGKIFLALQGNGQSIYVGVGEDAYMISSELYGLVEIAPR
                     FIRMNGEEAGGQLFILDQNRGTGAKGIAACCYDGAPIHLEERNLQTAEITTRDIDRGC
                     FPHFFLKEISESVRSVQGTLRGKYRITGAASAPSGILFNLGEDMVPASIRNGLQSGLT
                     RNIFVIGHGTAAVAGQAVADALAHCLGMSRLNIQSRVA"
BASE COUNT         2049 a         2505 c         2410 g         2005 t
ORIGIN      
        1 gctgaggctt ttgcgcggcg agatctccgc gcgtattccg atcagcctca tgtctcaata
       61 cacgcccgtt ccaaaagtgc gctcgcatcc gcttttgggc cgcagggtta cggcctcgga
      121 atatcatgaa attgtggaat ttgcccttag gctcgggttt gaaaatctat tcattcagga
      181 agtcgatgac agagccctga cgccggattt caagcgggag catcccttca attaatgtca
      241 attaatggga cgctgttaac ttattaactt tctattcaat atatttgtat ttattacgta
      301 tttaataaat tcaaacgttt aattatggtt gttttgcatc agttcaaacc cgttggattc
      361 ggcaatctgc cggcctcgaa caactgattt gctgatggcc ggaatggata agccgagacg
      421 acgggccaaa ccggccatcg tctctccaag atcacgaacc gcccaaaagc aaaacaggct
      481 tcgggcgttg acgatatgtg gttgcttacc cgccgtacgg acgtcaccgg gtttgacccc
      541 cgtcaatgcc gaaacgcgca aggccacttg atccagatcc acgccgctgt ttcgaagcgc
      601 ataacttttc ttgatcttct cttccgtctt tgacaaaacc tgactgacga aatcaccgtc
      661 acccagtatc ctttcatcgc ttcgttggta agtcccttcc tcgcatttcc gcttgagacc
      721 ctcccagccg ccagcgctgc ggatcaagcc cccgcctgtc aaatcatgcc gtctgccttg
      781 ttcaattccc tgcccgacaa actgtcgata ggactgacga gctgcatcct gcgtgttgcc
      841 aaaaagcatc agaattcctt gcgtttcctg ccaggaacga tgctctcttc ccatcagggc
      901 gctatggccg gaatacggat agctgtccag cttattcaaa ttctgaacca gtccggcccg
      961 cagcgggttg aggtgaatgt agcgtaccag ttcaagaaga taggcatcct cctggcataa
     1021 aatggacttg aatcgattct gaaaaaggtg gccgtgccgc tttcggctcc ggttaaacca
     1081 agtggcgtat ccggtcaaca gtcgcctcat cacggtcgaa atcggaatat tgcccgtgcg
     1141 gcaaagcaga tgaaaatgat tgggcatcaa tgaccaagca aaacaagtcg ttgcggtatc
     1201 cggaaaaata tctcctaacc gataaaggaa attatcccga tcggcatcag aatcgaatat
     1261 cttgccgctc tcgatgccgc gtgccatgat gtggtggacg gcgtcggctg catctatacg
     1321 cgctcctctt ggcatggcgt tttcaatatt ccgaattttc cgcgttgtca acgagaaagt
     1381 taataagtta acagcgtccc ctacatttta aaaatgctga cgccgggcgg aggctggaac
     1441 tggaatagtt tcgaggaaag cccgctgttc attttgatgt tggagaatct caggagagtg
     1501 gagttgccca tcacgtcgtc aaaaccgacc tggagaatgt aaaaattgtt ttttgccacc
     1561 gtcattctca gaaaggagta ggacgctcct ttttcccggg gataaaggag cagcaggcag
     1621 ttgccgtccg gatcctccgc gggagaggcg tattgaatcg tgaagtcatt attcagcttc
     1681 cccagaccgg acagaaattt gattaaggct tcggatttca taatctttcc cgaatcctgc
     1741 acgtaaacgg ttttatcctg gggcaggtag agccatgcct tctgagcgtt gatgatcagt
     1801 tttttcgcct tcggcttttt gtaatcccag agcatctgac cgggattctt gaaataaacc
     1861 gttcccgatt ccgtgtccgt tttccgaatg gacttcaccg tggtttcctg gataaaaccg
     1921 gcttgaaaat cctgcgtttt ttcataaatc cgctgaagcc ggtcaaccac ttcctcaagg
     1981 ggcggggccg ctgtggaagc accgggaaaa acaaccgccc aaaagagagc caggcaaaac
     2041 gccaggacgc cggccgggaa aaacaattca attttattga gctttgcctt catgtttttt
     2101 tatttcccgc aacagagaaa accctctcgg taatggcaca tttttttcaa tgcagtttat
     2161 atcttgggcc cgagaaagcc ataagtcgtt atgattaaaa ggtatatggg taactattga
     2221 atattactca tataaattat tgcttaaatc ttaagcaatc cggtccaaaa tccgaagcaa
     2281 tgcggacttg accggattat ccacacggtt gtcaacaggc ctttccacag ctttgtggaa
     2341 tccggttttc cttcagggcc aaaagcgcct aaatggttta aaacttgatt ttttacgcca
     2401 tcttcagtca atctgcattc tccagccaaa gggatcttct ctcgttccgt actgaatttg
     2461 cagtatttca ttgtatagct tttcggccag ctccccggtc tttccatcgt taatgagata
     2521 ttcccggttg tgataaaaaa tctgtccgac cggtgaaatc acggcagccg ttcccgtggc
     2581 aaacgcatcg cgcagcgttc cattttcaat cgcggagatc acctccgtga tggtcagccg
     2641 gctttcctcc accttcacgc ccaggtgcgc cgccagtttg atgacggaat cgcgcgtcac
     2701 gcccggaaga atggttcctc ccagcggagg cgtaatcaac cggtcttcga tcaggaagaa
     2761 gatattgctg gtgcccactt cctccacgta ccggttttcc ctggcgtcca gccacaggac
     2821 ctgcgtatag cccatcgtgc tggcaatgcg gctcgcgtag aggcttgcgg cgtaattccc
     2881 cgccgccttg acatgcccag tgccgcccgg cgcggcccgc acgcagtcgt cggacacata
     2941 aattttggtc ggcccgaagc cgtgcgcgta gtaggcgccc acaggcccgg caatgatgaa
     3001 gaacagatat tcgctcgccg gatgcacgcc gagagccggc tccgtggcaa tcatcgtcgg
     3061 ccggatatac agggacgacc ccttctcgcg gggaatccat tccttttcca gcacaacgaa
     3121 ctgccgaagc gcgttcagca cgaccgtttc gtccatgggc ggcatgcaca gcctttcggc
     3181 ggacacattc attctgcgga tattctccat cggacggaaa agataaattt cgttgtttgc
     3241 cccccggtac gccttcagac cttcaaagat ttcctggccg taatgaaggc acatggcggt
     3301 tggatccaac tgcaggtaat ggtagggttc gatgaccgga tcctgccatc cgtctccttc
     3361 cctgtacttc atggtaaaca tgtggtcggt gaaatgctga ccgaatccca gttttttttc
     3421 gtccggcttg ggcttcaggt acttgggaag cgctttgata attttaattt tcataggatc
     3481 ctcttggcaa aaagattccg gccattcggg gccggcttcg tttttttata gcactaaacg
     3541 atgttccgat caaggcttcg atactgaatc gcctcggcga cgtgcgcggc ttcgatcttc
     3601 gcggcgccct ccagatcggc aatggtgcgc gacactttca ggatgcggtc cagggcgcgg
     3661 gcggaaagtc ccagcttatc gacggccatt tccatgagct gatgcgactc ttcatcgatg
     3721 gggcagaaag aacggatctg cttttccgtc atggaggcgt tggtcggaat gcccgcgccg
     3781 ccgaagcgct ccttctggcg gcggcgagcc cggaccacgc gctctctgac atgggcggaa
     3841 gattcatttt cctgttttcc cgagagcgcc ctgtactgga tggacggcac ctcgacatga
     3901 aggtcgatgc ggtccagcag ggggccggag atttttgcct gatactgccg gatctgctgg
     3961 ggcgagcagc ggcagcggcg gacccggtct ccaaaatatc cgcacgggca ggggttcatg
     4021 gcggccacca gcatgaatct cgccggaaag gccgccgtta cggccgaacg ggtaatcgtg
     4081 atcgcgcctt cctcaagcgg ctgacggagc gcctccagcg cgtttctttt gaactccgga
     4141 agttcatcca aaaacaatac gcccagatgg gacagcgata tttccccggg atgcggcatc
     4201 tgaccgccgc cgaccaggcc cgcgtctgaa atggtgtgat gcggcgctcg aaagggacga
     4261 gccgccacca gcgtttcccc cgcattcaaa agtcccgcca cggaaaaaat cttggttacc
     4321 tcgatcgctt cttcaaagga aagctccggg aggatcgtgg gcagtcttcg ggccagcatg
     4381 cttttgccgg agcccggcgg accgatcatc agaacgttgt gagagcctgc cgcggcgatc
     4441 tccagcgcgc ggagcgcctg cttctggccc ttgatttcgg aaaaatcgat ttcgtaatga
     4501 cggtggactt gaaaaagccg gtcggcattc acgctgagcg gttcgatgat tttccggtca
     4561 gccagaaact cgacgacgtc gggaagcgtc cggacgccgt aaacctgaat gccttccacc
     4621 atcgccgctt ccgcggcgtt ttcttcggga acaaaaacgg aacggattcc agattcccgg
     4681 gccttgaagg cggcaggcag tacgccccgc actcccttga tgctgccgtc cagcgacagc
     4741 tccccgataa agaaacaacc gtccagggcc gcttcttcaa tcagcgcctc cgcggccaga
     4801 atgcccaccg cgatcggcag atcaaagccg gttccctctt tgcggatgtc ggcaggcgcc
     4861 aggttcaccg tcacgtgatc gcgcggaaaa gcgtagccgg aattcttgat ggcggccttg
     4921 atccggtcgc ggctttcctt gaccgaggcg tccggcaggc cgacggtggc aaaatgcggc
     4981 aacccccggg agacatccac ttccaccgtc acaggatagg attccatgcc gatcacgctc
     5041 atgctctgca ctttgatgaa cacttttatt ctccggggaa gaattcgcgg cgcaaatact
     5101 agcgaatcca ttcatttata tcaagatttt aatttgcaaa aattcttctt cgccattctt
     5161 gactcggtaa agacgctatg ctagggttcg ctgaaattga agaaatccca ataaccgtca
     5221 aacaccgaaa agttccgggt caccatgaaa aaattatctc atatcaacga tgaaggtcag
     5281 gttcggatgg tggatgtgac ggcaaaaacc gcaacgcacc ggcaggcgaa agcccgcggc
     5341 caggtaacca tgaagccggc cacgatgaag ctgctggccg agggacgcat cgccaagggc
     5401 aacgtgctga ccgccgctaa aatcgcaggc attctggcag ccaaaaatac cggcggcatc
     5461 attccgctgt gccatcccct ggggctgacc ggcatggaca tcacctttgt cctcgaccga
     5521 aaaaaatcac gggtggtcat tgaatcgcag gtgcggacgg tcgggcaaac cggggtggaa
     5581 atggaagcgc tgaccgcggt gagcgtcgcg gcgctcacca tttatgacat gtgcaaagcc
     5641 gtggaccggg aaatggtcat cggcgagatt ctgcttctgg aaaaatccgg cggcaagagc
     5701 ggcgccttcg tccgaaaagc cgcaaaataa ttcggagaca acgcgtgaac gctgtttaca
     5761 aaatcaggaa agctttccgt tatcctttcg tcacgctgac ggtcctgctg ttcgttctgt
     5821 ttctgacggg gctggcgcgc ggcgaatgct gggaaaaaat cctgacgggc gctttgtttg
     5881 ccgccgcgct ggtcgtggcc gttgaatcct cggagcggga attcggcatt tccgaaaacg
     5941 gcctgcggat tcgaaaattt ttccggacca ggcatttcac ctgggcggaa atcacgcacc
     6001 tgggcacggt catcctgcgc agcaaggcct atttcctgct cacgacgacc aagggatttt
     6061 atctcttctc caacctcctg caggaccaca cgcgcctgct cgggcagctc gcggagaagc
     6121 tggaagatga aagagtggaa acggaaatca aaaattacat cgaagccccc aaagaacgga
     6181 catcgctcat cgtgctgacc tgggtcgccc tggttctttt ggttgccgtg gtgctgaccc
     6241 gatggctcgc ggcctgatgt tttcacgcgg atcgtccgga attctgaagg gaacggttgg
     6301 aatgaaacaa tgttttgaaa cgcaggagct gcatgaacat gaaagcgaaa aagcagatta
     6361 acccggccgc gggaatagac gttcaggagg ccctcaacaa ggccgtcgaa gaaattctgg
     6421 aaaaaaagct ggatgatctg gcgacggtcc tgtcgggctc cgacaacggc agaagcaaac
     6481 ttcacgcgga ggtcatggcg atggtggaaa cgggcctttt caaaatcgct ttaaggcgct
     6541 ccggcaacgt caaaagctcg gcggcggcat ttctcggcat ccaccgcaat acctttaccg
     6601 ataaaatggc caagctgggc ctcgacggcg aaaaagaata gagattctct tcccatccat
     6661 ccctgaaatg atttaccccg catcggagcc tctccgcccc ggggaatgct ccgccctgaa
     6721 actctcccgc acaggatctt cagggtcgca tcccttatcc gctttcccaa tccaaaagag
     6781 aaaggcctcg gctttttcat cttcggcgtc gcaccaggcg gaatcggcct gagaaattca
     6841 ggaatggttt ttgcgtaatg catccggcaa agccccctga aaagcaggga gcggaagaat
     6901 tggacggcgc caaatccatc atcgcaatcc ccaggggcgg cgcgaatgac tcagccgcgg
     6961 catggggact gatgttcttg tcgacaaaac cgccgcggcc ggtttcgttt tacaaccgcc
     7021 ccgcaacagt tcaagaggaa atgatgttct atacaaaatt gtctcttctg ataaaaagat
     7081 ggctcttttt tgtaacacat tgccgggttt ttttcggcat tcggccggaa tcagttggcg
     7141 gacctgcaat cattttttct cccatcctcc ccggcagatt gaattgcgga ttcgcaggcc
     7201 tgctgtcctt tcaaccggcg ccggagaatc ccggcgatcc ggcggatcaa acccttcgga
     7261 agctttggga aaaagcaaat aagaacgggc tggaacaggt ctttggcgga aaaaccgacc
     7321 cgggcggtta tttgaacggc ccggaaacga tccagtctct cgcggattgc gtcaaccggc
     7381 tgaagcggga agatgtccag gaatgtcttt tttttcagaa agaccggacg gcggatttga
     7441 cccgcgtcgc cggcgggatg aaagcctttc tggccgcgga agagaaaatt ctggaggacc
     7501 gggcggcagg gctgagctcc gccggccttg aaaccgtcaa ccgccggctg ctctcgctca
     7561 aggacatttg ctggatgctg gagagcgaca tcctggccaa cctgcaaaaa gttctctcgc
     7621 tttccggagc ggaaaaatat tcttcgcttc aaccggccgc ctttcgaaaa taccggaagc
     7681 tgaatctgct gttaaacgcg ctggatcgtc tggaggtccg gggacgggac tcggcgggca
     7741 tccagttggc ttttttcctc aaagacgcaa aggtccttga ggaactggac cggcggatcg
     7801 aagaaaacgg atggggggag gattaccggc gccggacgca aaaaggcgat ctcataaacc
     7861 ggtccatttt tatttccggc ggcgctcaaa atcccggcgg cggaattcat attgccttta
     7921 cctacaaaac cttttccgtg gtcggcgaac tgggccgcaa cgtcgccgac ctgcggcaat
     7981 ggatctcccg cgacgccatt cttcaatggt ttgccgcacg ggcgtcggcg gacgaatccg
     8041 ccatcgccca cacccggtgg gcctccgtgg gctccatcac cgaagaaaat tgtcatccgg
     8101 tcaacaacgc aacccttcgt ccggaattgt ccgatttccc cgcgtatccg gggcgggaag
     8161 cgcacatcag cgtggtgctc aacggcgaca tcgacaatta tccggcgctt cgcagggacc
     8221 tggagcaaga cggggagcaa atcgcgccgg acgtctcgac ggacaccaaa atcattcccc
     8281 tgcaaatcga aaaatatctg agaaaagggc aaaacctgac agacgccttc aggctggccg
     8341 taaacgattt cgaaggttcg cacgccattg ccatgcaaag caatctggag ccgggcaaaa
     8401 tatttctggc gcttcagggg aacggccagt cgatttacgt cggcgtcgga gaagacgcgt
     8461 acatgatttc ctcggagctc tatggactgg tggagattgc accccgcttc atccggatga
     8521 acggcgagga ggcgggcgga caactgttca ttctggatca aaaccgaggc acgggcgcaa
     8581 agggcattgc cgcctgctgc tacgacggag ccccgattca tctggaagag cggaatctgc
     8641 aaaccgccga aatcacgacc cgggacattg accgcggctg tttcccccat ttctttctca
     8701 aagaaatttc agaatccgtc cgttccgtcc agggaacgct gcgggggaaa taccggatca
     8761 ccggcgccgc ctccgctccg tccgggattc ttttcaatct gggcgaggat atggttcccg
     8821 cctcgattcg aaacggcctg cagagcggct tgacgcggaa catcttcgtc atcggccacg
     8881 gcacggcggc ggtggccggc caggcggtgg cggatgcgct ggcgcactgc ctcggcatga
     8941 gccgtctcaa tatccagtcg cgcgtcgcg
//
LOCUS       DAOSIM010000061         8817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_7493_length_8817_cov_7.294910, whole genome shotgun sequence.
ACCESSION   DAOSIM010000061 DAOSIM010000000
VERSION     DAOSIM010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8817
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7493_length_8817_cov_7.294910"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            306..932
                     /locus_tag="PLR47_04175"
     CDS             306..932
                     /locus_tag="PLR47_04175"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOG12169.1"
                     /translation="MPGPDSIQKRQEELAQRQWPIADIEARFNRLAQNGIPHKTPDRE
                     KMLRDREEILDRVQRRAEDYCYLMRNCAKSSATALFEEFGLGSLDIIRGLGPFPGLAM
                     SGGICGPVAGGLIALGLFFSHPDITKQDDTGAYLYGRLFIHRFESAFGSLSCPDIQKR
                     LLGKYYDPMSSMENLKAFNASGARKQCVIAPGMGARIAAEIMLESMAK"
     gene            1012..2046
                     /locus_tag="PLR47_04180"
     CDS             1012..2046
                     /locus_tag="PLR47_04180"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOG12170.1"
                     /translation="MIQLKFDQKVCASCRSADCLLKCQYMTFSGHKEAHGEMMKVMKG
                     KDSRVLTECVTCYACEEYCTRGNHPFYLISEKREEKGMFTAPRPITTQWKNMTQMQGK
                     CLVGNVKDTALSCCYLPELGQLGTGEIFKDVASAGVFGAEFMCPAVHSHFARMSVIKE
                     RLPIVIENYRKLGVKEVICMHDECYGTLTSIASAYGMEVPFKPVYYLDFVLQRLKELK
                     DKIKPLGITVAYQRSCSNRLIPDKLPVVKKIFKLIGVKAPKRLYQDENALCCAEILRS
                     VSGYKLADDVQKRNIDDMLAVGAEYCVFNCPACQTSLSEKVVKQGLKPVHIIDLCKMA
                     IGEKEREAVS"
     gene            2043..3425
                     /locus_tag="PLR47_04185"
     CDS             2043..3425
                     /locus_tag="PLR47_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOG12171.1"
                     /translation="MSDIRKSLVDIVGERGVSDAPEELWFYSRDPGVLAPHRPDYVVA
                     PKTTEEVQKIVQLAGRENIPVVPMGNGMSLAGLVIPLKGGIVLDMKRMNKILEVNHMA
                     RYVVVEGGTSQGALKAYLQKNHPTLRHSIPDAPSTTTIAANVSLHGQGRLTNQYGFNS
                     DMVTGLEVVLPTGEICRIGSPSIGPYWISKGPTLPDLSGLFLGWLGCTGVITKVGLKL
                     YPNKKIRDAELFVTDRVDLIPHILYKLTHLEMLEDINVWFQPKPLMFMGNYHITVFFT
                     GDTEEEVEFKRKMVWDCLQEYMDSKDGGFMSIQYMKSMLLEMPGRNIADFADVPKGGG
                     FEYSGPIAPIEHFPQYAAKVVELAAKYNVLYASTARLISGGHSMMYSISFAFNRSDTA
                     MMERVEQALTEATAFALDMGGIPWKPNFKEQKMTLEKMEPNARKLLLMIKKNLDPQGI
                     MNPGNWEVNS"
     gene            3422..4597
                     /locus_tag="PLR47_04190"
     CDS             3422..4597
                     /locus_tag="PLR47_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOG12172.1"
                     /translation="MTYIRHIEHENIVHRCFRCGYCKFPSDYSDFNCPSYKAFGWDTF
                     SPGGRMWLTRAWLSGEIETSARFAEIMFSCVACDNCKDQCVFPRFKDFLPDIFQETRA
                     ELVGEGRIPPGVRDYFKAVAISGNPYKLPQSERGDWARGTGLKPFTNQKHLFYAGCVG
                     SYDEVGKRMAQSVGKMLVDAGLSVGILGSEETCDGNDVRVLGEMGLFAELAQANIEKF
                     KAKGVKEIITLDPHALNTFKKDYPALGGVFQVRHYSEVLAELVKKKKIKLKAYPVKIT
                     YHDPCYLGRHQSIYQAPREILKAIPKAEFVEMHRSGPDAFCCGGGGGNFFTDILGTAE
                     DSAARVRVREAAETGARILAVACPNCAKMLSDAVKMENLEDRLELLGIAEILERVRK"
     gene            4813..5337
                     /locus_tag="PLR47_04195"
     CDS             4813..5337
                     /locus_tag="PLR47_04195"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOG12173.1"
                     /translation="MNKEEMLEEVFKRAKACELRGGNCAQCSLNAIFEVLGVEDENVV
                     RSATGFADGVGLSGDGHCGALSGGTMAISYFFGRKKEDLGRMGKQIRALLLAKKFHEQ
                     FVKEFSTCRCHDLQVRQFGRFFNLYDMQDMKAALAAGMPEGCSTLVGKAARMALAIIL
                     DEQARGDLGSNLYS"
     gene            complement(5445..6413)
                     /gene="queG"
                     /locus_tag="PLR47_04200"
     CDS             complement(5445..6413)
                     /gene="queG"
                     /locus_tag="PLR47_04200"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOG12174.1"
                     /translation="MKAASTGKNFQSTDRGDPATLAAAIKEEARRLGFDGCGISRAEA
                     LPEDALRLCSWLERGHHAGMAYMERQPEKRVDPTLLFQGARSVVSVLLNYYPSGTQTD
                     PEAPVLAKYAYGKDYHVVVREKLNRLLKKISSGIAPVRGRAFVDTAPLLEKAWAARAG
                     LGWIGKNTCLISPGKGSFCFIGTLLIDIPLQYDRPVADLCGDCTLCIDACPAKAIVAP
                     RTLDAGHCISYLTIESRPENSGMPNHRLAGRVFGCDICQDVCPWNRSAAPHHEKDFDA
                     PPGLIEMTREDWFNLNEEKYKRILQHSAVQRIGFEGLKRNLERIAG"
     gene            complement(6461..7300)
                     /gene="mtgA"
                     /locus_tag="PLR47_04205"
     CDS             complement(6461..7300)
                     /gene="mtgA"
                     /locus_tag="PLR47_04205"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOG12175.1"
                     /translation="MQKWKKIILWSFLAVAVFITLDVGRYFFYPDVAGLQKDRPGKTA
                     FMEYREEIWKEKGINKKISVSWVPLSAVSPYVMKAVIIAEDDKFWSHEGFDFDAMQKA
                     LEKDIKKKKFKAGGSTISQQLAKNLYLTPAKNPVRKIKEAILTWRIERQLSKRRILEL
                     YLNVAEWGDGLFGVGTAARRYYGKSAAGLGPREAAHLAAALPNPNRFRPGGSSRYAAN
                     RAERIYQIMVRRGIVIEEYDEVLKDEGGEEEKAPEPVSAEQEASVPVEPAQTEAPEPA
                     VLP"
     gene            7555..8496
                     /locus_tag="PLR47_04210"
     CDS             7555..8496
                     /locus_tag="PLR47_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOG12176.1"
                     /translation="MMQRRTIVSWGLFALFLLVCFHIDAHAVQYTVKKGETLSLISKR
                     TGVSVAEIKKANHLTGNTIKRGQILSIKEKKSPSPVTAKSKIQPTPSFYKVKRGDTLP
                     LIAQKTGVPVTQIAKLNHVTTRTLRAGQKLMLPKEEPIANALPEEEDDLLDEEDDGLT
                     DDLSGAIACEDKAGEELLGKWGSAEERKLFIRVATGFLGAPYRLGGATVKGIDCSAFV
                     RKMYEFFDITLPRTAREQSTVGMQVKRDELVEGDLVFFRTKKPIGHVGIYIGNNEFVH
                     ASYKAKAVRIDSLDRPYFQKRFQRAVRLKGLDENGGT"
BASE COUNT         2071 a         2510 c         2361 g         1875 t
ORIGIN      
        1 tccggctggt acgacgtcaa aaaatggatt ctttcctttg gagcggatgc cgagcttttg
       61 gagcctgccg acaaaagaaa ggaagtcctt caagagatca gccggacggc ctcgctatac
      121 cgatcaggta taaaaccggc agggcgacgc attgaaagat gatgattcga accctcgtgc
      181 acatcagttc ttattcctca ctggcaatga aaaccaccac ccctttactt tgaactggct
      241 ctttggaaag aaagtgtgct atgttttctt ccatccaaag cgacacatga agaggagagc
      301 caaccatgcc gggtcctgat tccattcaaa aacgccagga agagctggcc caacgccaat
      361 ggccgatcgc cgacattgaa gcacgattca accgattggc gcaaaacggc attccacaca
      421 aaacgccgga ccgggaaaaa atgctccgcg acagggaaga gattctggat cgcgtgcagc
      481 gccgggcgga agattactgt tacctgatgc gcaactgcgc caagagcagc gccacggccc
      541 tgttcgaaga gttcggtctg ggcagcctgg acatcatccg cggattgggt ccgtttccag
      601 gcctggccat gtccggcgga atctgcggac cggtggctgg agggctcatc gccctgggtc
      661 tttttttctc ccatcccgat atcaccaaac aggacgacac cggcgcgtat ctttacggac
      721 ggctgttcat ccaccggttt gaatcggcat tcgggagcct ctcctgcccg gatattcaaa
      781 aaaggctgct gggaaaatac tatgatccca tgagcagcat ggaaaatctc aaggcgttca
      841 acgccagcgg cgcccgaaaa cagtgcgtga tcgcgccggg catgggcgcc aggattgccg
      901 cggaaatcat gctggaaagc atggctaaat aaagcgtcca gcccctgccg gacaggagcg
      961 ggcgtcaaat cgggttttat ggatcccatc attcatcgag ggaggtgctt catgattcaa
     1021 ctgaaattcg atcagaaggt ttgcgcaagc tgccgctcgg ccgactgcct gctcaaatgc
     1081 cagtacatga cctttagcgg gcacaaggaa gcgcacgggg aaatgatgaa agtcatgaag
     1141 ggaaaggatt cccgcgtgct gaccgaatgc gtcacctgct acgcctgcga ggaatactgt
     1201 acgcgcggca accacccctt ttacctcatt tcggagaagc gcgaggagaa aggcatgttc
     1261 accgcgccgc gccccatcac gacgcagtgg aaaaacatga cgcagatgca gggaaagtgc
     1321 ctggtgggca acgtgaagga cacggcgctt tcctgctgct acctccccga actggggcag
     1381 ttgggaacgg gggaaatttt caaggacgtc gcctcggcgg gcgtcttcgg cgcggaattc
     1441 atgtgccccg ccgtgcactc gcactttgcc cgcatgtccg tcatcaagga gcggctcccc
     1501 attgtcatcg aaaactaccg caagctgggc gtcaaagaag tcatctgcat gcatgacgaa
     1561 tgctacggca cgctgacctc gattgcctcc gcctacggca tggaggtgcc cttcaagccg
     1621 gtttactatc tggatttcgt tttgcagcgc ctgaaagaac tcaaggataa aatcaaaccg
     1681 ctcggcatca ccgtcgcgta ccagcgctcc tgctccaacc ggctcattcc ggacaagctg
     1741 ccggtggtga aaaaaatctt caagctcatc ggcgtgaaag ctcccaagcg tctctatcag
     1801 gacgagaacg cgctttgctg cgcggaaatt ctccgctccg tttccggcta caaactggcc
     1861 gacgacgtgc aaaagcgcaa catcgacgac atgctggccg ttggcgccga atactgcgtt
     1921 ttcaactgtc cggcctgcca gacctctttg tcggaaaaag tcgtcaaaca gggattgaag
     1981 ccggtacaca tcatcgatct gtgcaagatg gcgatcggcg aaaaagaaag ggaggccgtc
     2041 tcatgagcga tattcgaaaa tccctcgtgg atattgtcgg cgagcggggc gtctccgacg
     2101 ctccggaaga actctggttt tattcgcgcg atcccggggt gctcgcgcct cacagacccg
     2161 actatgtggt cgcgcccaaa accacggaag aagtccagaa gattgttcaa ctggcaggcc
     2221 gtgaaaatat ccccgtcgtt ccaatgggga acggcatgtc gctcgcgggc ctggtgattc
     2281 cgctcaaggg cggcatcgtt ctggatatga agcggatgaa caaaatcctg gaagtgaatc
     2341 atatggcgcg ctacgtggtc gtagaaggcg gcacttccca gggcgcgctc aaagcctatc
     2401 tgcagaaaaa ccatccgacg ctccggcaca gcattcccga cgccccttcg accaccacca
     2461 tcgccgccaa cgtttcgctg cacggccagg gaaggctcac caaccagtac ggtttcaact
     2521 ccgacatggt cacggggctg gaagtggtcc tgcccacggg cgagatctgc cgaatcggtt
     2581 cgccgtcgat cggcccctac tggatttcca aagggcctac gcttcccgat ttgtcggggc
     2641 tcttcctggg ctggctcggc tgcacaggcg tcattaccaa ggtgggcctg aagctctacc
     2701 ccaacaaaaa gatccgggac gcggaactct tcgtcacgga ccgggtcgat ctgattccgc
     2761 acatcctcta caagcttacc cacctggaaa tgctggagga catcaacgtc tggttccagc
     2821 ccaaaccgct catgtttatg gggaattacc acatcacggt tttcttcacg ggtgacacgg
     2881 aagaagaagt cgaattcaaa aggaagatgg tctgggactg ccttcaggaa tacatggatt
     2941 ccaaagatgg cggcttcatg tcgattcaat acatgaagtc catgctcctg gaaatgccgg
     3001 gccgcaacat cgccgatttt gccgatgtgc cgaagggcgg cgggtttgaa tactcggggc
     3061 ccatcgcgcc gatcgagcat tttccgcaat acgccgcaaa ggtggtggag ctggccgcca
     3121 aatacaatgt cctctatgcc agcacggccc ggctcatttc cggcgggcac tccatgatgt
     3181 acagcatctc ttttgccttc aaccgcagcg acaccgccat gatggagcgc gtggagcaag
     3241 cgcttaccga agcgacggct tttgccctgg acatgggcgg cattccctgg aagcccaatt
     3301 tcaaggagca gaaaatgacg cttgaaaaaa tggaacccaa cgcgcgcaag ctcctgttga
     3361 tgatcaagaa aaatctcgat ccgcagggga tcatgaaccc cggcaactgg gaggtgaact
     3421 catgacctac attcggcaca tcgaacatga aaacatcgtc catcgctgct tccgctgcgg
     3481 ctactgcaag ttcccttcgg attacagcga cttcaactgc ccttcctaca aggcctttgg
     3541 ctgggatacc ttctcgcccg gcggccgaat gtggctcacc cgcgcgtggc tgtccggcga
     3601 aattgaaacc tccgcgcgct ttgcggaaat tatgttttcc tgcgtggcct gcgacaactg
     3661 caaggatcag tgcgtgtttc ccaggttcaa ggatttcctg ccggacatct ttcaggaaac
     3721 ccgcgccgaa ctggtgggcg aaggacggat tccgccgggt gtgcgcgact acttcaaggc
     3781 cgtcgcgata agcggcaatc cctacaagct cccgcagagc gagcggggcg actgggcccg
     3841 cgggacggga ctgaagcctt ttaccaacca gaagcacctc ttttacgcgg gctgcgtcgg
     3901 ctcctacgat gaagtcggaa agcgcatggc ccaaagcgtg ggaaaaatgc tggtggatgc
     3961 gggcctgtcg gtcggcattc tcggcagcga agagacctgc gacggcaacg acgtgcgggt
     4021 tctgggcgag atgggactgt ttgccgagct cgcccaggcc aacattgaaa aattcaaggc
     4081 aaaaggcgtc aaagaaatca tcacgctcga tccgcatgcg ctcaatacgt tcaaaaaaga
     4141 ctaccccgcc cttggcggag tcttccaggt ccgtcactac agcgaggttt tagccgagct
     4201 ggtgaaaaag aaaaaaatca agctcaaagc gtatccggtc aagatcacct accacgatcc
     4261 ctgctaccta ggaaggcatc agtccattta ccaggcgccg cgcgaaatcc tgaaggctat
     4321 tcccaaagcc gagtttgtcg aaatgcatcg cagcggtccc gatgctttct gctgcggcgg
     4381 aggaggcgga aatttcttca cggacattct gggaacggcc gaagacagcg ccgcccgcgt
     4441 gcgtgtgcgc gaagccgccg agacgggagc tcggattctg gcggtcgcct gccccaactg
     4501 cgccaagatg ctctccgacg cggtgaagat ggaaaatctg gaagaccgac tcgagctgct
     4561 gggcatcgct gaaattctgg agcgggtccg gaaatgactt tggttccctc tcccttcgat
     4621 gggagttgcg gaaaaacggc aactctgcgc ctgtatgtca ggaactgcct ttggtaccct
     4681 ctcccttcga tgggagaggg ttagggtgag ggtgaaatca aatccccctc cccccagtcc
     4741 ccgcccacca ggggcgggga agcatctaag gaatattaag cctgaaaaaa taataacgga
     4801 gatcataatc aaatgaacaa agaagaaatg ctcgaagaag tctttaagag ggcgaaagcc
     4861 tgtgaacttc gcggcggcaa ctgcgcgcaa tgctcgctga atgctatttt tgaagtcttg
     4921 ggcgtggagg atgaaaacgt cgtgcgctcc gccacgggat ttgccgacgg cgtgggcctt
     4981 tccggcgacg gccactgcgg cgcgctttcc ggcggcacga tggccatcag ctactttttc
     5041 ggccggaaaa aagaagacct gggccggatg ggcaagcaga tcagagcgct gctgctcgcc
     5101 aaaaaattcc atgaacagtt cgtaaaggaa ttctccacct gccgctgcca cgatcttcag
     5161 gtcaggcagt tcggccgctt tttcaacctc tacgacatgc aagacatgaa ggcggcgctg
     5221 gccgcgggaa tgccggaagg ctgctccaca ctcgtgggca aggcggcgcg catggcgctg
     5281 gccatcatcc tcgacgaaca ggcccgcggg gacttggggt caaatcttta ttcttgacat
     5341 ttgaccgtca cgcttccgtt ttcaccagtc gtcgactggc tttgcgtaaa tatttcaaaa
     5401 atatcccccg cgctggaaaa aaacacagtc ttcccgtgac cggattatcc cgctatgcgc
     5461 tcgaggttcc ttttcagccc ctcaaatccg attctttgca ccgcggagtg ctgcaatatt
     5521 ctcttgtatt tttcctcgtt gaggttgaac cagtcctcgc gggtcatttc aatcaagccg
     5581 ggcggtgcgt caaaatcttt ttcatggtgc ggggcggcgc tgcggttcca ggggcagaca
     5641 tcctggcaga tatcgcagcc gaacacgcgg ccggccaacc gatggtttgg catcccactg
     5701 ttttccgggc ggctctcgat cgtcaggtag gaaatgcagt ggccggcatc cagcgtccgc
     5761 ggggcgacaa tggccttcgc ggggcaggca tcgatgcaga gcgtgcagtc gccgcagaga
     5821 tcggcaacgg gacggtcgta ttgcagggga atatcgatca gaagcgttcc aatgaaacaa
     5881 aaagagcctt tccccggtga aatcaggcag gtgtttttgc cgatccaccc cagccccgcc
     5941 cgggcggccc acgccttttc gagcagcggc gccgtatcca caaacgcccg gccgcgaacc
     6001 ggcgcgatgc cggatgaaat tttcttcagc agacggttca gtttttccct gacaacgacg
     6061 tgataatctt tcccgtaggc gtactttgcc aaaaccggcg cttccggatc ggtttgcgtt
     6121 cctgacggat agtaattcaa aagaacggaa acaaccgagc gagcaccttg aaacagcagg
     6181 gtcggatcaa cacgcttctc cggttgcctt tccatgtagg ccattccggc gtgatgcccg
     6241 cgttcgagcc aagagcacag gcggagagcg tcttccggaa gcgcttcggc gcgggaaatc
     6301 ccgcagccgt caaacccgag ccggcgggcc tcctccttga tcgcggcggc cagcgttgcc
     6361 ggatcacccc ggtcagtgga ttgaaaattt tttcctgtgg acgctgcttt cattgatttt
     6421 atcccgtttt ccaatcctga cgcccgcggt ttcaagactg tcagggaaga accgcaggtt
     6481 cgggcgcttc cgtctgcgcc ggctcaacgg gaacagaggc ttcctgctcc gccgataccg
     6541 gctcaggggc tttttcttct tctccgcctt catccttcag aacttcatca tactcttcaa
     6601 tgacaatgcc gcgccgcacc atgatctgat aaattctctc cgcccggttg gccgcatatc
     6661 ttgaactgcc tcccggccga aaacggttcg gattgggcag agccgccgca agatgggccg
     6721 cttcacgcgg tccgaggccg gcggcggatt tgccgtaata tctgcgcgcg gcggtcccca
     6781 caccaaacag gccatccccc cattcggcca cgttcaaata gagctccaga attcttctct
     6841 tgctcaactg cctttcgatt cgccaggtta aaatggcttc cttgattttc cgcaccggat
     6901 ttttcgccgg cgtcagatat aaattctttg ccagctgctg ggaaatcgta ctgcctcccg
     6961 ccttgaattt cttctttttg atgtcttttt ccaacgcctt ctgcatcgcg tcaaagtcaa
     7021 atccctcgtg agaccagaat ttatcatcct cggcaataat caccgccttc attacataag
     7081 gcgacacggc cgacaggggc acccaagata cggatatttt tttgttaatg cctttttcct
     7141 tccatatctc ttcccggtat tccatgaagg cggttttgcc gggacgatcc ttctgcagac
     7201 cggccacgtc cggataaaaa aaatagcggc cgacatccag cgtgataaaa acggccacag
     7261 ccaaaaaact ccataaaatt atctttttcc acttttgcat gagcgcactt tcctcttgtt
     7321 tttcgtttgc cgcttcctat acctcattga aaaagattgc aaaataaatt tgacatgccc
     7381 ttccattttt agtaaggggg actcgtacga agttgataag ctgtcgttta attgtaaact
     7441 ttcgttttct gtgtgcagta aaatatcttt gatatacaaa tagtagcttc accgatccac
     7501 aatctgtgca ccgttaacaa tatggggctc cccccgctaa acaggaggta cttcatgatg
     7561 caacggcgaa ccatagtatc ttggggatta tttgctcttt tcctgctggt ctgttttcat
     7621 atcgacgccc atgcggtcca gtataccgtt aaaaaaggtg aaaccctctc cctcatttcc
     7681 aagcgaaccg gcgtcagtgt ggcggaaatc aaaaaagcca accatttgac cggcaatacc
     7741 ataaaacgcg gccagatttt aagcatcaaa gaaaaaaaat ctccatcccc cgtcaccgca
     7801 aaatcaaaaa ttcaacccac tccatccttc tataaagtta aaagaggcga cacgcttccc
     7861 ctgattgccc aaaaaaccgg cgttcccgtt acgcagatcg caaagctgaa tcatgtcacc
     7921 accagaaccc tgcgcgccgg acagaagctg atgctgccca aagaggaacc gatcgcgaat
     7981 gcgctccccg aggaagagga cgatttgctg gacgaagaag atgacggatt gacggatgac
     8041 ctctccggcg caatcgcctg tgaagataaa gcaggcgagg aacttttggg caaatggggc
     8101 agcgccgaag aaagaaaact gttcatccga gtggcaacag gctttctggg cgcaccttac
     8161 cggctgggag gcgccaccgt caagggaatt gactgttcgg cctttgtccg gaaaatgtac
     8221 gaatttttcg atatcactct gccccgcacc gcccgggaac aatccacagt cggcatgcag
     8281 gtgaagcgtg atgagctggt ggaaggggac ctggttttct tccggaccaa aaagccgatc
     8341 ggccacgtcg gcatttacat cggcaacaac gagttcgttc acgcctctta caaggcaaag
     8401 gccgtccgca tcgacagcct ggatcgaccc tattttcaga agagatttca gcgggccgta
     8461 cgtctcaaag gcctggatga aaatggcggc acctgattgt gtttcgtgac tcaaaaggta
     8521 ttcaaaaaga ggcttgaacg cctctttttt ttattggccg ttttgccggt tgcttatccc
     8581 ggccgtttat tatatgagga caatcctgcc ggttcccgta tcgaatgggt gaaagactgc
     8641 tgaatcaact ggaataatcg caatgtgccg caaggaagaa ccaatggaaa aaaacatcgt
     8701 ggaaaaaatt ctggccgaac atctggtgga cggcaacctc gcgcccggcg gggaaatcgg
     8761 catccgcatc gaccagaccc tcacccagga cgccacgggc accatggctt atctgca
//
LOCUS       DAOSIM010000062         8761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_7534_length_8761_cov_7.965886, whole genome shotgun sequence.
ACCESSION   DAOSIM010000062 DAOSIM010000000
VERSION     DAOSIM010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8761
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7534_length_8761_cov_7.965886"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..>552
                     /locus_tag="PLR47_04215"
     CDS             103..>552
                     /locus_tag="PLR47_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOG12177.1"
                     /translation="MLFDYILGRFSNDLAIDLGTANTLVYVKGKGIVLNEPSVVAVHK
                     NHHGVKKVLAVGNEAKSMLGRTPGNIVAIRPMRDGVIADFDITEAMLKHFILCVHDRK
                     SLVRPRIIVSVPSGITQVERRAVRETVESAGAREVYLIEEPMAAAIGS"
     assembly_gap    553..562
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <563..1109
                     /locus_tag="PLR47_04220"
     CDS             <563..1109
                     /locus_tag="PLR47_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOG12178.1"
                     /translation="IGGGTTEVAVISLSGIVYAKSVRMAGDKIDDEIVQYMKRKYSLL
                     IGERTAELIKTEVGCAYPMAEIKTIDVKGRDLISGIPKTIEVSSDEIREAIIEPVTMI
                     VDTIKDALENAPPELAGDIVDRGIVLTGGGALLKNLDILVREETGLPVTVADDPLSAV
                     ARGAGMALDQLDVLKEVTIQV"
     gene            1160..2005
                     /gene="mreC"
                     /locus_tag="PLR47_04225"
     CDS             1160..2005
                     /gene="mreC"
                     /locus_tag="PLR47_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOG12179.1"
                     /translation="MFFKNYRAVLFVGAVILAALFLFSYGLKYDVGQSLVQRLALGAA
                     APVQKLFTLCVESVGNAWTRYIRLVGLEEENRKLKTEIGELEARMTLYRQGHLEAQRL
                     QALLSLAEREPYRFIAARVIGREQAALAKTLWIDKGEAQGLKPGMPVMALPGLIGRLT
                     NVSLHSARVQLLIDEGSSADVLVERTRQQGMLRGAGSRGCVIRYISKIQDVREGDVIL
                     TSGLSNIFPKGLLVGKVVHTDRADAGMFLNIRVAPFADFTSLEEVLVLAGEKKDPFRE
                     RKAAK"
     gene            2002..2490
                     /locus_tag="PLR47_04230"
     CDS             2002..2490
                     /locus_tag="PLR47_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12180.1"
                     /translation="MMYYILLPFMAVVLVVLQSKLAEILFSGLVTLELSLIVVIYAGF
                     RLELWKGAALSLVVGLAMDCLSGSLLGLFTLLYVFIFLLAWFVSSQIDSQRLYLIAGF
                     SLVCSAAESLALVLLYQWVFGFDMVDKILPVLAPQTLLVSVLSVCFFYAMRRVERLLA
                     YG"
     gene            2483..4387
                     /gene="mrdA"
                     /locus_tag="PLR47_04235"
     CDS             2483..4387
                     /gene="mrdA"
                     /locus_tag="PLR47_04235"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOG12181.1"
                     /translation="MDKPGRLQNGPEPVDFRRKLAVVMILVCAALSVLMIRLWYLQIV
                     KGDELKQRSENNAVRFRRIQPLRGLIMDRNGFVIVDNRPSFDVLYLPARGVDHEALLA
                     KLQDLYAVKSLEFPEGQTLPRTLKSYLPVRLEKNVGMEKVALVETNSLDLPGVYVDVT
                     PVRLYLDGEVIAPIIGYTGEVSREELEKPDGDYASGDITGKSGIEKALDSYVRGRRGN
                     ELVEVNVTGKVIKNLGRIDPVPGSNVVLTIDAALQKSAWEAFEGRPGAAVAMDPQTGA
                     ILAMVSSPSFDPNLFNSGIAREEWEKLQKDPLKPMSNRAIAGQYPPGSTYKLIVAAAA
                     LEEGVITPETRIHCNGSFVLGNRTYRCWRKNGHGPVNLHRALVESCDVYFYTVGKKLG
                     VDRIADYAKRFGLGDATGVELAHERKGLVPTKSWKLARVKEPWQMGETISISIGQGFN
                     LTTPLQLAQAYAALANGGTLWRPHLVQRIEAPQGGVVREYPPEKKGEIGLDGKIAALL
                     RQALWGVVNENGGTGYAARIAGADVCGKTGTSQVVGLPQDEQARRRKKIAAFHKDHAL
                     FVCYAPARRPEIVVSVVAEHAGGGGAVAAPIARRILKAYFDGKKNERPRQAAAGSQTP
                     PPSGNGKPQG"
     gene            4403..5515
                     /gene="rodA"
                     /locus_tag="PLR47_04240"
     CDS             4403..5515
                     /gene="rodA"
                     /locus_tag="PLR47_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOG12182.1"
                     /translation="MFFKYDRRIFQNFDKTLFALVMSICAIGVVNIYSTGFSLGENAT
                     PLYLKQIQWIAFGLFFMMVAFLIDYRYFNRAAYGLYAFSMASLVAVALFGHTANGAQR
                     WISFGVFLFQPSELMKISIILALARYFDDHKSHEPYRLRELLVPFGMVMLPCALILKQ
                     PDLGTALMIVLVSASIVLFIGMNWKSIVIAVASVMVLMPPAWHFLKDYQKNRLLTFLS
                     PESDPLGTGYHIIQSMIAIGSGGFLGKGFLKGSQTQLKFLPEQQTDFVFSVFAEEWGF
                     LGGLALMVIFTSLIIWGLKIALHSKDLLGTILAFGITALIAWQVLINMGMVLGVLPVV
                     GIPLPFISYGGSAVVSLMAAIGLLMNVSVRRFILQR"
     gene            5549..5926
                     /locus_tag="PLR47_04245"
     CDS             5549..5926
                     /locus_tag="PLR47_04245"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOG12183.1"
                     /translation="MWEKKKRLDDTKAFLGKGAEFIGKLIFTGHVRIDGDFQGEIFGQ
                     GAVDVGEGASVKADIEVGSIFIAGEVQGNIAVKEKINIHSTGKLAGDVKAPVFVMEEG
                     AVFDGQSRMSEAKEQKRIALAGK"
     gene            6217..6651
                     /locus_tag="PLR47_04250"
     CDS             6217..6651
                     /locus_tag="PLR47_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HOG12184.1"
                     /translation="MVTVIPDYTLLVQMATFIALIFILNFLLYKPLLSILERRKKQLD
                     ELGNEIKLFNDSVNKKAAEYDEKLSKAKTSASDLKKEIIGEGAAEAKRVVDAVRSEIP
                     LMTQEFQKKMDAEMQAARQILDGQSRRLSLEIAEKVLGRRVQ"
     gene            6648..7241
                     /locus_tag="PLR47_04255"
     CDS             6648..7241
                     /locus_tag="PLR47_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HOG12185.1"
                     /translation="MKSSFRSFRLSILLSLAFVLLSAALAFASGGGEGGHGGTQWKDF
                     AWKTLNAAMIIGFLVYLLGPKIKGFFAGRRQEIKESLESATAQKAEAEKQYREYAEKI
                     DKASQEIDGIFEMIKAQGVVEKDKIIEDAHKVAQKMKEDAQARIEQELKKASGQLRAE
                     AVALSVRMAEEILKKNITAQDHEAMVREYMDKVVNKH"
     gene            7238..7780
                     /gene="atpH"
                     /locus_tag="PLR47_04260"
     CDS             7238..7780
                     /gene="atpH"
                     /locus_tag="PLR47_04260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOG12186.1"
                     /translation="MISNISKRYARAFFEIAGEEKKLEQYYNELHQFSSVIAANKDLE
                     GFLANPIFEQESKKKVLEKIIGKLSLSPMTINFLKLLIDKKRIDVLPDIETCYRQLMD
                     ETLQKVRVTVKTAFPLSGEMQSYIASSLKQMTGKSVDVTVEDDKNLLGGIVIGVGDTL
                     YDGSIKSQLNNMRNLLGEAR"
     gene            7784..>8761
                     /gene="atpA"
                     /locus_tag="PLR47_04265"
     CDS             7784..>8761
                     /gene="atpA"
                     /locus_tag="PLR47_04265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004723422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOG12187.1"
                     /translation="METIKAEEISQIISEQIKSYEKKLDISETGTVLSVGDGIARVYG
                     VQNAMAMELLEFPGGILGMVLNLEADNVGVAVLGEVTHIKEGDIVKRTGKIAQVPVGE
                     ALLGRVIDATGAPLDGKGPIEASEFRRIEMVAPGVIQRQPVNQPMYTGLKAVDAMTPI
                     GRGQRELVIGDRQIGKTAICVDAIIRQKDTGIKCIYVAIGQKKSTVAQVVERLKQHDA
                     LSYTCVVAGCASDPATLQYIAAYAGCSIGEYFRDRGQDALIIYDDLSKQAVAYRQISL
                     LLRRPPGREAFPGDIFYNHSRLLERAARVSKELGGGSLTALPIIETQAGD"
BASE COUNT         1900 a         2312 c         2702 g         1837 t
ORIGIN      
        1 gcaaatacat tttaaaaagg cgttgcttag gccgttgaaa tacgatatag acgcgaaact
       61 atagataaat acgatatatt caaggagtat tcaggaggat tcatgctgtt cgattatatt
      121 ctgggaaggt tttccaatga tctggcgatt gatctcggaa ccgccaatac gctcgtttac
      181 gtgaagggaa agggaatcgt gctgaacgaa ccttccgtgg ttgccgtgca taagaatcat
      241 cacggcgtca agaaggtgct ggccgtcggc aacgaggcca agagcatgct gggccgcacc
      301 cccggcaata tcgtggcgat ccgtcccatg cgcgacggcg tcatcgcgga tttcgacatc
      361 acggaggcga tgctcaagca tttcattctg tgcgttcacg accgcaaatc cctggtgcgc
      421 ccccgcatca ttgtttccgt gccctcgggg attacgcagg tggagcgccg cgcggtgcgc
      481 gagacggtcg aatcggccgg cgcccgcgaa gtctatctga tcgaagaacc gatggcggcg
      541 gcgatcgggt cgnnnnnnnn nncatcggcg gcggcaccac ggaagtggcg gtcatctcgc
      601 tgtccggcat cgtgtatgcc aagtcggtgc gcatggccgg cgacaagatc gatgatgaaa
      661 ttgttcagta catgaagcgg aaatacagtc tgctcatcgg cgagcggacg gcggaattga
      721 tcaagacgga agtcggctgc gcctatccga tggcggagat caaaacgatc gacgtgaagg
      781 ggcgcgatct catctccggc attcccaaga cgatcgaagt cagctccgat gaaatccggg
      841 aagcgatcat cgagccggtg acgatgatcg tggacacaat caaggatgcg ctggaaaacg
      901 cgccgccgga gctggccgga gacatcgtgg accgcggcat cgtgctgacc ggcggcggag
      961 cgcttttgaa gaatctcgat atcctggtgc gggaggaaac cggtcttccc gtcacggtgg
     1021 ccgacgatcc gctcagcgcc gtggctcgcg gcgccggcat ggcgctggat cagctcgatg
     1081 tgctcaaaga ggtgacgatt caggtctgac gccgcctggc cgcccggtct tccaaggcgg
     1141 cggatagttt gggtagatca tgttttttaa aaattatcga gccgtcctct ttgtcggagc
     1201 ggtgattctc gccgcgcttt tccttttttc gtacggcctg aaatacgatg tcggccagag
     1261 cttggtgcag aggcttgcgc tgggggcggc ggcgccggtt caaaaactgt tcacgctgtg
     1321 cgtcgaaagc gtcggaaacg cctggacgcg ctacatccgc ctggtgggtc tggaagaaga
     1381 aaaccggaaa ctgaagacgg aaatcggcga gctcgaggcg cggatgacgc tctaccggca
     1441 gggacatctg gaggcccagc ggctgcaggc gcttctgtcg ctcgcggagc gggagcccta
     1501 ccgctttatc gcggcccgcg tgatcggcag ggagcaggca gctctggcca agaccctctg
     1561 gatcgacaag ggagaagcgc aggggctgaa gccgggcatg ccggtgatgg cgcttcccgg
     1621 actcatcggg cggctgacga atgtttccct gcacagcgcc agggttcagc ttctgattga
     1681 cgagggctcc agcgcggatg tgctcgtcga aaggacgcgt cagcagggaa tgcttcgcgg
     1741 agcgggatcg cgcgggtgcg tcatccggta catctccaag attcaggacg ttcgggaagg
     1801 ggatgtgatt ctgacgtccg gcctgagcaa catttttccc aaggggctgc tggtcggaaa
     1861 ggtggtccat acggaccggg cggacgccgg aatgtttctg aatatccggg tggctccttt
     1921 tgccgatttt acctcgctgg aggaggtgct ggttctggcg ggggagaaaa aggatccgtt
     1981 ccgggaaagg aaggccgcaa aatgatgtat tacattctgc tgcctttcat ggccgtcgtg
     2041 ctggtggttc tgcagtccaa gctggccgaa attctttttt ccggcctcgt cacgctggag
     2101 ttgtcgctga tcgtggtgat ctacgccggc tttcgcctgg agctgtggaa gggagcggcc
     2161 ctttcccttg tcgtggggct ggcgatggat tgcctgtccg ggtcccttct cgggctgttc
     2221 acgctgctgt atgtcttcat tttcctgctc gcctggtttg tttcctcgca gattgattcg
     2281 caaaggcttt acctgatcgc cggcttcagc ctggtctgtt cggcggcgga atcgctggcg
     2341 ctggttctgc tctaccagtg ggtgtttggc ttcgacatgg tcgataaaat cctccccgtc
     2401 ctggcgccgc agaccctgct ggtcagcgtt ctgtcggtgt gcttctttta cgcgatgcgc
     2461 agggtggaaa gattgttggc gtatggataa accgggcaga cttcaaaacg ggccggagcc
     2521 ggtggacttt cggcgcaagc ttgccgtggt gatgatcctg gtttgcgcgg ccctgtccgt
     2581 cctgatgatc cgcctgtggt atctgcagat cgtcaagggg gacgagctca agcagcgctc
     2641 ggaaaacaac gccgtccgct ttcgcaggat acagcccctg cgcggcctga tcatggaccg
     2701 caacggtttt gtgatcgtgg acaaccgccc ctcctttgac gtgctgtacc tgcccgcacg
     2761 gggggtcgat cacgaggcgc tcctcgcgaa gcttcaggac ctctacgccg tcaaatccct
     2821 tgaatttccc gagggccaga ctcttcccag aacgctgaag tcctatcttc ccgtccggct
     2881 ggagaaaaac gtcggcatgg aaaaagtggc cctggtggaa accaacagcc tggatctgcc
     2941 gggggtctat gtggatgtca cgcccgtccg gctttacctg gacggggagg tcatcgcgcc
     3001 gatcatcggc tacacggggg aagtcagcag ggaggagctg gaaaagccgg acggggatta
     3061 cgcctcgggc gacatcacgg ggaaatcggg cattgaaaaa gcgctggatt cctatgtgcg
     3121 cgggcgccgg ggcaatgaac tggtcgaagt gaacgtgacc ggcaaggtca tcaagaattt
     3181 ggggcggatc gatccggttc ccggctccaa cgtggtgctg acgattgacg ccgcccttca
     3241 gaagtcggct tgggaggcct ttgagggaag accgggcgcg gcggtcgcga tggatccgca
     3301 aaccggcgcc attctggcga tggtcagctc gccgtctttc gacccgaatt tattcaacag
     3361 cggcattgcc cgtgaagagt gggaaaagct gcagaaggat ccgctgaaac ccatgtccaa
     3421 ccgggcgatt gccgggcaat atccgcccgg ctcgacctac aagctgatcg tggcggcggc
     3481 ggcgctggaa gagggggtga tcacccccga aacccggatc cactgcaacg gatcttttgt
     3541 tctgggcaac cggacctatc gctgctggag aaaaaacggg cacgggccgg tcaatctgca
     3601 ccgggcgctg gtggagtctt gcgacgtcta tttttacacc gtcgggaaaa agctgggcgt
     3661 ggatcgcatc gcggactacg cgaaacggtt cggcctgggc gacgcgaccg gcgttgagct
     3721 ggcccatgag cgaaagggtc tggttcccac gaagagctgg aagctggccc gggtgaaaga
     3781 gccgtggcag atgggcgaaa ccatatcgat ttccatcgga cagggattca atctgaccac
     3841 tccgctgcag cttgcgcagg cttacgccgc gctggccaac ggcggcacgc tgtggcgccc
     3901 tcacttggtg cagcggattg aagcgcccca gggcggcgtg gtccgggaat atccgcctga
     3961 aaaaaaggga gagatcggcc tcgacggaaa aatcgcggcg ctcctgaggc aggcgctgtg
     4021 gggcgtcgtg aatgaaaacg ggggcaccgg ctacgcggcg cgcatcgccg gcgcggacgt
     4081 ctgcggcaaa acggggactt cgcaggtggt cggtctgccg caggatgaac aggcgcgccg
     4141 ccggaaaaaa atcgcggcgt ttcataagga ccatgcgctg tttgtctgct acgcgccggc
     4201 caggcgtccg gaaatcgtag tatccgtcgt tgccgagcac gccggcggcg gcggcgcggt
     4261 ggccgcgccg atcgcccgca ggattttaaa ggcgtatttc gacggcaaga aaaatgagcg
     4321 gccccggcag gcggctgcag gttcgcaaac gccgccgcct tccggaaacg gaaagccgca
     4381 agggtaagca cggggaggct ttatgttttt caaatacgat cggcgcatct ttcagaattt
     4441 cgacaagacg ctgtttgccc tggtgatgtc gatctgcgcc atcggcgtcg tcaatatcta
     4501 ttccaccggc ttcagcctgg gcgaaaacgc gacacccctg tatttaaaac agattcaatg
     4561 gattgccttc ggcctgtttt tcatgatggt cgccttttta atcgattacc gctacttcaa
     4621 ccgcgcggcg tacggccttt acgcgttttc gatggcgtcg cttgtcgcgg tcgcgctgtt
     4681 cggccacacg gccaacgggg cgcagcggtg gatctcgttc ggcgtttttt tattccagcc
     4741 ttccgagctg atgaaaatca gcattatcct cgccctggcc cggtattttg acgatcacaa
     4801 gtcgcatgag ccttaccgcc tgagggagct cttggttccg ttcgggatgg tgatgcttcc
     4861 ctgcgctttg attttgaaac agccggatct ggggacggcg ctgatgatcg ttctcgtttc
     4921 cgcttccatc gttctgttca tcggaatgaa ctggaaatcc atcgtgatcg ccgtcgcgag
     4981 cgttatggtc ctgatgccgc cggcctggca ttttttaaaa gattatcaga aaaaccgcct
     5041 gctgacgttt ctgtctcccg aaagcgaccc gctgggcacg ggataccaca tcattcagtc
     5101 catgatcgcg atcggctccg gggggtttct gggcaagggc tttttgaagg gctcccagac
     5161 ccagctcaag tttctgcccg aacagcagac cgacttcgtc ttctccgtgt ttgccgagga
     5221 gtggggattt ctcggcggcc tggcgctgat ggtgattttt acgtccctca ttatctgggg
     5281 attaaaaatt gccttgcatt caaaagattt attgggcacg atactggcgt tcggcattac
     5341 cgcgctgatc gcctggcagg tgctgatcaa tatgggaatg gtgctgggcg tcctgccggt
     5401 cgtcggcatt ccgctgccgt tcatcagcta cggcggttcg gcggtggttt ccctgatggc
     5461 ggcgatcgga ctgctgatga atgtgagcgt gcgcagattc atattgcagc gctgaggatt
     5521 ttcgtttcgg gaccgcaagg agcagggcat gtgggaaaaa aagaaacgtc tggacgacac
     5581 caaggctttt ttgggaaagg gcgccgaatt catcgggaag ctgattttta ccgggcacgt
     5641 gcgcattgac ggcgatttcc agggggagat tttcggacag ggggcggtgg atgtcgggga
     5701 aggcgcttcg gtcaaggccg atattgaagt cggcagcatt tttatcgccg gcgaagtcca
     5761 gggcaacatc gcagtaaagg aaaaaatcaa catccactcc acgggcaaac ttgcaggcga
     5821 cgtgaaggcg ccggttttcg tcatggagga gggggcggtt tttgacggcc aatcgcgcat
     5881 gagcgaggcg aaagagcaaa aaagaatcgc tttggcagga aaataattta catcaagcat
     5941 tcaaaatcat tagcataata ttggttttgc caaaattaga ccgccggtca taaaaaagct
     6001 tgacaaaccc tatgcgggtg tgattagtaa cctccgtcct cgcggtgtgg ctaggttcag
     6061 cagaagcccg gcttgatgca agccttacgt tgtctgaagc aaatcactcc cagagattac
     6121 gctgtttgaa atgtcggatc aaatttaatg catcttcctg cgcaaatcag cgttcgttga
     6181 aggccgggaa ggtctggaaa agagacgagg tcagttgtgg ttaccgtaat ccccgattat
     6241 acgctcctgg ttcagatggc tacgttcata gccttgattt tcatccttaa ttttcttctt
     6301 tacaaacccc tgttatccat tctggaacgc cgcaaaaagc agttggacga gctgggcaac
     6361 gagataaagc ttttcaatga ttccgtgaat aaaaaagccg ccgaatacga tgaaaagtta
     6421 agcaaggcca agaccagcgc ctccgacctg aaaaaagaaa tcatcggcga aggcgcggcg
     6481 gaagccaaac gcgtcgtgga cgccgttcgc agtgaaatcc ccctcatgac ccaggagttt
     6541 caaaagaaga tggacgcgga aatgcaggct gcccgtcaga tcctggacgg ccagtcccgc
     6601 aggctttcgc tggaaattgc cgaaaaggtc ctgggaagga gagttcaatg aaatcttcat
     6661 ttcgttcctt taggctttca atccttcttt cgctggcttt cgtattgctt tccgcggccc
     6721 tggcttttgc ctccggcggc ggcgaggggg ggcatggcgg cacgcagtgg aaagactttg
     6781 cctggaaaac tctgaacgcc gccatgatca tcgggttttt ggtgtatctg ctgggcccga
     6841 aaatcaaagg tttctttgcc ggccgccgtc aggaaatcaa ggagtctctg gaaagcgcca
     6901 ccgcccagaa ggcggaagcg gaaaagcagt accgggagta tgcggaaaaa atcgacaagg
     6961 cgtcccagga gatcgacggc atctttgaaa tgatcaaggc gcagggcgtg gtcgaaaaag
     7021 acaagatcat cgaggacgcg cacaaggtcg cccagaagat gaaggaagac gcccaggcgc
     7081 gcatcgagca ggaattgaaa aaagcgtccg gccagcttcg cgcggaagcg gttgccctgt
     7141 cggtgcggat ggcggaagaa attctgaaga aaaatattac ggcacaggac catgaggcca
     7201 tggtcaggga atatatggat aaggtggtga acaaacattg atcagcaata tatcaaaacg
     7261 atacgcccgc gctttttttg aaattgccgg ggaagaaaag aagctggagc agtattacaa
     7321 cgaactgcat cagttctctt ccgtgatcgc cgcgaacaag gatctggagg gcttcctggc
     7381 caatcccatc tttgaacagg aaagcaagaa gaaagtgctg gaaaaaatca tcggcaagct
     7441 ttcgctttcc cccatgacga tcaatttcct gaaattgctg atagacaaga aaaggatcga
     7501 cgtccttccg gacatcgaga cctgctaccg gcagttgatg gacgaaacgc ttcagaaggt
     7561 gagagtcacg gtgaagacgg cgtttccgct gtccggggag atgcagagct acatcgcgtc
     7621 gagcctgaag caaatgacgg gcaagtccgt tgatgtgacg gtggaagacg acaagaacct
     7681 gttgggcggg atcgtgatcg gtgtcgggga tacgctgtac gacggcagca tcaaaagcca
     7741 attgaataat atgaggaatc tcttagggga ggcaagatag aacatggaaa ccatcaaggc
     7801 ggaagaaatc agtcaaatta tttctgagca gatcaaaagc tatgaaaaaa agctggatat
     7861 aagcgaaacc ggaaccgtcc tgtccgtcgg tgacggcatc gccagagtgt acggcgtgca
     7921 gaatgccatg gccatggagc ttctggagtt ccccggcggc atcctcggca tggtgctcaa
     7981 cctcgaagcg gacaatgtcg gtgttgccgt tctcggcgaa gtgacccaca tcaaagaagg
     8041 ggatatcgtc aaaagaaccg gcaagattgc ccaggttccg gtcggtgaag cgcttttggg
     8101 ccgcgtgatt gacgcgacag gcgcccccct ggacggcaaa ggcccgattg aagcctcgga
     8161 gttccgccgc attgaaatgg tcgcccccgg cgtgattcag cgccagccgg tcaatcagcc
     8221 gatgtacacg ggcctcaagg ctgtcgatgc catgaccccg attggacgcg gccagcgtga
     8281 actggtcatc ggcgaccgcc agatcggcaa gacggccatt tgcgtggatg ccatcattcg
     8341 ccagaaagac accggcatca agtgcattta tgtcgccatc ggccagaaaa aatccaccgt
     8401 ggcgcaggtc gtggaaaggc tcaaacagca tgacgcgctg tcctacacct gcgtggtcgc
     8461 aggctgcgcc tccgacccgg caacccttca gtacatcgcg gcgtacgccg gctgcagcat
     8521 cggcgaatac ttccgtgacc gcggccagga cgcgctcatt atttatgatg acctgtccaa
     8581 acaggccgtg gcctatcgtc agatttcact gcttttgcgc cgtccgcccg gacgggaagc
     8641 cttccccggc gacattttct acaaccactc gcgtcttctg gagcgcgcgg cccgcgtgag
     8701 caaggaactg ggcggcggat cgctcacggc tctgcccatc attgaaacgc aggccggcga
     8761 c
//
LOCUS       DAOSIM010000063         8659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_7631_length_8659_cov_6.119247, whole genome shotgun sequence.
ACCESSION   DAOSIM010000063 DAOSIM010000000
VERSION     DAOSIM010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8659
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7631_length_8659_cov_6.119247"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..1557)
                     /gene="hydF"
                     /locus_tag="PLR47_04270"
     CDS             complement(310..1557)
                     /gene="hydF"
                     /locus_tag="PLR47_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOG12188.1"
                     /translation="MDNTPKANRLHIALLGRTNVGKSSLLNLMLGQDIAITSSVAGTT
                     TDVVEKAMELLPIGPVLFLDTAGLDDVSELSGARLQKTARVFDRVDVIILVAEADTWT
                     NYEDTVLSEAQKRKIPFLIVVNKIDLRKPAPDWMNMLTRKAGPVLTVSCNDETGRQAY
                     LDALKQQLLEKAPADFIVTPSLIGDLLPPGGFAVLVVPIDLQAPKGRLILPQVQTIRD
                     TLDNDATAVVVKERELADTLANLKNPPAIVVTDSQAILKVTADVPQHIPVTTFSILFA
                     RQKSDLAVMAAGAAAIEHLKPGDRVLIAEACSHHALEDDIGRVKIPRWLRQYVGGDLQ
                     IDTASGRDYPADLKDYKLVLHCGACMINRREMLTRLHRAQDAGVPVTNYGVAISFLQG
                     VLKRSLALFPFALLALEKEMGNS"
     gene            complement(1550..1681)
                     /locus_tag="PLR47_04275"
     CDS             complement(1550..1681)
                     /locus_tag="PLR47_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12189.1"
                     /translation="MKEFSAGAEKNMRKFLEQKLEKEIVEQHLSPFALTALGYRKNG"
     gene            complement(1739..3502)
                     /locus_tag="PLR47_04280"
     CDS             complement(1739..3502)
                     /locus_tag="PLR47_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOG12190.1"
                     /translation="MVTVKLTIDNRELEAPVGATILEAARIAGIKIPTLCASPEIKHT
                     PGACRVCMTEVEGQRSLIAACVFPVFEGMVVHTNTEKVRKARKMVVELLLANHPQECS
                     HCVRNGNCELQKVAEFVGLKEIRFPFTEFPQKENFIDRSSPSIVRDSRKCIECHRCVT
                     VCEQIQTVSVLTPAFRGSNIKVTPAFDLPLVESNCVACGQCIMACPVGALYEKDDVDK
                     VWAALQDPAKHVIVQEAPAIRAALGEEFGMPPGSLVTGKMIAALRRLGFDKVFDTNFT
                     ADLTIIEEGNELLKRVKEGGTLPMITSCSPGWIKFCEHFYPDLLPHLSTCKSPQQMFG
                     ALAKTYYAETANIDQKNIVSISIMPCTAKKFECQRPEMNSSGFQDVDYVLTTRELGRM
                     IKEAGIDFENLPEEDYDAPMGEYTGAATIFGATGGVMEAALRTVYAVVTGENLPSLDI
                     TPVRGLDGVKEATVKVGPLGDVKIAVAHGLGNARKLMDLIREGKGGYAFIEVMCCPGG
                     CIAGGGEPIPTNNDIRVKRSAALYKDDGAVQKNRQSHENESVKKLYEKFLKEPLGHKS
                     HKLLHTKYTQRGTEMPHKRDE"
     gene            complement(3519..4556)
                     /gene="hydE"
                     /locus_tag="PLR47_04285"
     CDS             complement(3519..4556)
                     /gene="hydE"
                     /locus_tag="PLR47_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOG12191.1"
                     /translation="MLLLKAEIGKPLSREEMVELLKMPGEYSVDLFAAADRVRENNVG
                     NEIYLRGIIEFSNFCERNCLYCGLRKTNEKLERYRMSGDEILETAREIKATGIATVVL
                     QSGEDSHYTAERICKLVQAVKEETDLVITLALGERSPEDYRAFFDAGADRYLLKHETA
                     SPELYKYLRPGCRLDARVSCLKMLKNLGFEAGTGNMVGLPGQTLEILADDLLLMKLLD
                     ADMLGIGSFIAHPDTPLAGIDNDDIELALRVIAIARLLTRNTNIPATTALATLNPHGR
                     LQALQAGANVVMPDFTPEIYRVRYEIYPGRKNDLQSADSIAQLEKDLHRIGRTMNYGT
                     GQRNKHRPIKS"
     gene            complement(4567..5964)
                     /gene="hydG"
                     /locus_tag="PLR47_04290"
     CDS             complement(4567..5964)
                     /gene="hydG"
                     /locus_tag="PLR47_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOG12192.1"
                     /translation="MIKDFIDDDRIQNILNQTINPDPLHVREVIDKAYELKGLTPEEV
                     AVLLQTEDENLIGAILQAAHKIKQDIYGNRLVLFAPLYVANHCSNNCLYCGFRRDNKE
                     LNRVALNLDQIRKEVEVLEHEGHKRLLMLCGEHPSRSSLDYFIESIEAAYATKTENGG
                     EIRRINVELAPLEVDEFRRLKKTGIGTYLLFQETYHHETYKKMHPSGPKKDYVNRLTA
                     MHRAQEAGIDDVGLGALFGLYNYKFDLLGLLFHALQLEKDCGVGPHTISIPRLEPAFN
                     APAAIQPPMPVSDRDFKKLVAILRMAVPYTGMILSTREKPELRSEVFNYGVSQISAGS
                     RTNPGGYSDTTSDRYRAAQFNLGDTRTLDEVILDITERGHISSFCTACYRLGRTGKDF
                     MDLAKPGLIQKFCQTNAVFSFKEYLLDYASPKTKKAGEKLIAEMLEKSFKSQRKKMLI
                     NRLQTIEEGKRDVYI"
     gene            complement(6039..6701)
                     /locus_tag="PLR47_04295"
     CDS             complement(6039..6701)
                     /locus_tag="PLR47_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOG12193.1"
                     /translation="MMHHEKMILKHPWRVRMFHYLLIVSFLPLAVTGVILYFKPLAEE
                     SMNFAMQIHVAAGVVLTLDAAAFFLLAFDRVILFIERIFTFSMDDVRWFAILGGYPQK
                     MLLHKKVAVPPMGKYNSGQKLFGICVFIGGTVLILSGLVLWAFPHLTPRAAVAYLGTL
                     HTFFAWVLTLFLCVHLFLGVYMFDDFKAMILHGKISYEEAKEMAPRWVEKEIVHIAEK
                     QS"
     gene            complement(6713..7033)
                     /locus_tag="PLR47_04300"
     CDS             complement(6713..7033)
                     /locus_tag="PLR47_04300"
                     /inference="COORDINATES: protein motif:HMM:NF014327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HOG12194.1"
                     /translation="MEEKSYQYMENPLHVTRREFITIGGIVIAFLALPAVWFKSIATS
                     NNQYIQARTKGLYQDDEKSAVRVSHANQSVMRYYKEFGGEPLGHLSHELLHTGYINRS
                     KGLI"
     gene            complement(7052..8329)
                     /locus_tag="PLR47_04305"
     CDS             complement(7052..8329)
                     /locus_tag="PLR47_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOG12195.1"
                     /translation="MNVAAKTYQKGEIPGVIAIKASVCKGCDACKRHCPTGAITGMFG
                     ATHSINADLCLSCGQCLINCPFGAPYETSDTVDAVIAKLQDKNTTVVGIIAPAVRVAI
                     GEDFGLPAGSLVTGKLYGSMRKAGFDILDNNFTADLTIMEEGSEFIARARHAVFGEEN
                     AHGGHIGPLPQFTSCCPAWVRFVETKYPALIPHLSSAKSPQQMAGTVAKTYGVKVWNK
                     PADRIYTVGIMPCTAKKLEASRPEFISAYEYNSKKGQAAGASYPDVDTVLTTRDLSRL
                     FKKLGINLAAEKEMEGGTLLADYTGAGTIFGNTGGVMEAALRTAYAVITGNELKPLEF
                     KKVRGLNGVKSASVTIRDSKYSKDVTINVAVVHGIARNIAPVVDEVLAGRSPYHFIEV
                     MNCPGGCINGGGQPIYGNGSSWIDAIRPWFAWR"
BASE COUNT         1901 a         2296 c         2294 g         2168 t
ORIGIN      
        1 gcttcatcaa cggcaacatc ggcgtcatga tcgatgcgct cgcctcccac caccaggccg
       61 aactgctcaa tcgccaggga taaggcaaac atccttttcg gaatgaaact tgtgaaaaaa
      121 aaccgcgcgc ctgaagctaa ccgaacaggc tagggacatc atcaaccgtt tttaaattct
      181 cgaaaaatac attgagcaac ttttacagaa gacaaatcag aactttacga aattgattcg
      241 taacaataag cagtaaatct acaaagcctc aggggtcctg tgtaacggtt gcattttgct
      301 tgatggatat cagctgtttc ccatctcttt ttccagagcc agaagtgcga aaggaaagag
      361 cgccaggctg cgtttgagca ctccctgcag aaaggagatc gccacgccgt agttggtgac
      421 gggcacaccc gcgtcctgcg ctctgtgaag acgggtgagc atttcacggc ggttgatcat
      481 gcaggcgccg cagtgcagga ccagtttata gtctttcaga tccgcgggat aatcgcggcc
      541 ggaggcggta tcgatctgca agtcgcctcc cacgtattgc ctcagccagc gcggaatttt
      601 cacgcggccg atgtcgtctt ccagagcgtg gtgggagcag gcttccgcaa tcaggacgcg
      661 gtctccgggc ttcagatgtt caatggccgc ggcgcccgcc gccatgacgg ccaggtcgga
      721 tttctgtctg gcaaacagga tggaaaaggt ggttaccgga atgtgctgcg gcacgtcggc
      781 ggttactttc aaaatcgcct gcgaatccgt caccacaatg gccggcggat ttttcaggtt
      841 cgccagtgtg tcggccagtt ccctttcctt gaccacaacg gctgtcgcat cgttgtccag
      901 cgtgtcgcgg atggtctgca cctgcggcag gatcagcctt cccttcggcg cctgcaaatc
      961 aatgggaacc actaaaacgg caaagccgcc ggggggcagc aaatcgccga tgagcgacgg
     1021 cgtaacgata aaatcggccg gagccttttc cagcagttgc tgctttaacg catccagata
     1081 agcctgccgc ccggtttcat cgttgcagga tacggttaaa accggtccgg cctttcgggt
     1141 cagcatgttc atccagtcag gcgctggctt gcgcaaatca attttattga caacaatcag
     1201 aaaaggaatt ttgcgttttt gcgcttcgga taaaaccgta tcctcgtaat tcgtccaggt
     1261 atccgcttcg gcaaccagga tgatcacgtc gacgcggtca aacactctgg ccgtcttttg
     1321 caggcgcgca ccggacagtt ccgacacgtc gtccagaccc gccgtgtcca gaaacaggac
     1381 cggcccgatc ggcagcagtt ccatggcttt ttccacgacg tccgtcgtcg ttccggcaac
     1441 ggacgacgta atggcgatgt cctgccccag catcaggttg agcaggctcg acttgccgac
     1501 gttggtgcgt cccagcaggg cgatatgaag acgattggct ttcggcgtgt tatccatttt
     1561 tcctatatcc cagcgccgtc agcgcgaaag gagagagatg ctgctccacg atttcctttt
     1621 ccagtttttg ttccaggaat ttgcgcatat ttttttcagc gcccgcggaa aattctttca
     1681 tcagttccgt ggcacggtca tattcaacca aaaatggttt cgacaagcaa gaggtttgtt
     1741 attcatctct tttatggggc atttccgtgc cccgctgggt gtacttggtg tgcagcagct
     1801 tatgggattt gtgtcccaga ggttccttga ggaacttttc ataaagcttc ttcaccgatt
     1861 cgttttcatg agactgacga ttcttctgga cggcgccgtc gtctttatag agagccgcgg
     1921 accgctttac gcgaatgtcg ttgttcgtgg gaatcggctc gccgccgccg gcaatgcagc
     1981 cgccggggca gcacatgact tcaataaagg cgtagcctcc cttgccttcg cggatcagat
     2041 ccatcaattt gcgggcgttt cccaggccgt gggcgacggc gatcttgacg tctcccagcg
     2101 gtcccacctt gacggtcgct tccttgacgc cgtcgaggcc gcgcacgggg gtgatgtcca
     2161 gactgggcag attctcgccc gtaaccaccg cgtaaaccgt ccgcagcgcc gcttccataa
     2221 cgccgcccgt cgcgccgaaa atggtggctg cgccggtata ttcgcccatc ggagcgtcat
     2281 agtcttcttc cggaagattt tcaaagtcga tgccggcttc cttgatcata cgccccagtt
     2341 cgcgcgtcgt aaggacgtaa tccacatcct ggaacccgct gctgttcatt tcggggcgct
     2401 ggcattcaaa ttttttggcg gtgcagggca tgatggaaat cgaaacaata tttttctgat
     2461 cgatgttggc ggtttccgcg taataggttt tcgccagggc gccgaacatc tgctgcggcg
     2521 atttgcaggt ggaaagatgc ggcagcagat ccggatagaa atgctcgcag aacttgatcc
     2581 agccggggct gcacgaggtg atcatgggca gcgtaccgcc ttctttaacg cgtttgagca
     2641 actcgttgcc ttcctcgatg atggtgaggt cggcggtgaa gttggtgtcg aacaccttgt
     2701 caaatcccag gcggcgcaga gctgcgatca tcttgccggt gaccagcgat ccgggaggca
     2761 taccgaattc ttcgcccagg gcggcgcgga tcgccggagc ttcctgaacg atgacatgct
     2821 tggcgggatc ctgcagagcg gcccagacct tgtccacgtc atccttttca taaagggcgc
     2881 cgaccgggca ggccatgatg cactgtccgc aggcgacgca gttggactca acgagaggca
     2941 gatcgaatgc gggtgtcacc ttgatattgc tgccgcggaa agctggagtt aaaacgctga
     3001 ccgtctggat ctgctcgcaa accgtgacgc agcggtggca ttcgatgcac ttgcggctgt
     3061 cgcggacgat cgaaggactg gatcgatcaa taaaattttc tttttgcgga aattcggtaa
     3121 aggggaaccg aatttctttg aggccaacaa actcggccac tttctgcagt tcacagttgc
     3181 cgttgcgcac gcaatggctg cattcctgcg gatggttggc cagtaagagt tccacaacca
     3241 tttttctcgc cttgcggact ttttccgtgt tggtgtggac aaccatgcct tcgaagactg
     3301 ggaaaacgca ggcggcaatc agcgaccgct gaccttccac ttccgtcatg caaacgcggc
     3361 aggcgccggg ggtgtgtttg atttccggcg aggcgcaaag cgtggggatc ttaatgccgg
     3421 ctatgcgggc agcctcaaga atggttgcac caacaggcgc ttctagctca cgattatcaa
     3481 tagtaagttt aacggttacc ataaatgatt gctccttttc atgatttgat tggtctgtgt
     3541 ttgtttcttt gtccggtgcc ataattcatt gttcttccta tgcgatggag atctttctcc
     3601 agctgggcga tgctgtccgc cgattgtaga tcattttttc tgccgggata tatttcatac
     3661 cggacacggt agatttccgg cgtaaaatcc ggcatcacaa cgtttgctcc cgcctgaagg
     3721 gcctgaagac gcccgtgggg attgagcgtg gccaacgccg tagttgccgg gatattggtg
     3781 ttacgggtca aaagacgggc aatggcgatg acgcgcaaag cgagttcaat atcgtcgtta
     3841 tcaataccgg ccagcggtgt gtccggatgg gcgatgaacg acccgatacc cagcatgtcg
     3901 gcatcgagca gtttcatgag caaaagatca tcagccaaaa tttcaagagt ctgtcccggc
     3961 agtcccacca tgtttcccgt ccccgcttca aatcccagat ttttgagcat tttcaggcat
     4021 gagacgcgtg catccagtcg gcagcccgga cgcagatatt tatacagttc cggcgaggcg
     4081 gtttcgtgtt tgagcagata acggtctgcg ccagcatcga agaacgcccg gtaatcttcg
     4141 ggcgatcttt ccccaagagc cagcgttatc accaggtctg tctcttcctt gactgcctgc
     4201 accagtttgc agatgcgttc ggccgtgtaa tgggagtctt ccccggattg cagcacaact
     4261 gtggcaatgc cggtggcttt gatctcgcgc gctgtctcca gaatttcatc tccggacatc
     4321 cggtagcgct ccagtttttc attggttttg cgcagaccgc agtaaaggca gtttcgttca
     4381 cagaaattgg aaaattcgat aattcccctc agataaatct cattgccgac attgttttct
     4441 ctgacacggt cggccgcagc gaaaagatcc acggaatact cacccggcat ttttaaaagt
     4501 tcaaccattt cctctctgct taacggtttg ccgatctccg ctttcaatag cagcatgtca
     4561 atgtggtcag atatagacat cccgcttccc ttcttcgatg gtttgcagac ggttgatgag
     4621 catttttttg cgctgggact taaacgattt ctccagcatt tcggcaatca gtttttcacc
     4681 tgcttttttg gttttcgggc tcgcatagtc cagcagatac tccttgaaag aaaacaccgc
     4741 attggtctga cagaatttct gaatgagacc aggtttggcc agatccatga agtctttgcc
     4801 cgtgcggccc agacggtaac aggccgtaca gaaactggag atatggccgc gttcggtaat
     4861 atcgagaatg acttcatcga gtgtgcgggt gtcccccagg ttgaattgag cggcgcggta
     4921 gcggtcgctc gtcgtatccg aatatccacc gggattggtg cgggagcctg cgctgatctg
     4981 ggaaacgccg tagttaaaaa cttctgaacg cagctcaggt ttttcccgtg tgctcagaat
     5041 cattcctgta tagggtacgg ccatgcgcaa aatggcgacc agcttcttga agtcgcgatc
     5101 ggaaacaggc atgggcggct ggatggccgc cggcgcgttg aatgcgggtt ccagccgggg
     5161 gatggaaatc gtatgcggac cgacgccgca gtctttttcc agttgcaggg cgtggaacaa
     5221 cagtcccagc aaatcgaatt tataattgta gagtccgaaa agcgcgccca ggcccacgtc
     5281 gtcgatgccg gcttcctggg cacggtgcat ggcggtcaac cggtttacat aatctttttt
     5341 ggggccggac ggatgcatct ttttgtaggt ttcatgatga tacgtttcct gaaaaagcag
     5401 gtaggtgccg atgccggttt ttttcagacg gcggaattcg tcaacctcga gcggagcaag
     5461 ttccacattg atgcggcgga tttctccacc attttccgtt ttggtggcat aggccgcttc
     5521 gatggattca atgaagtaat ccaggctcga acgcgacgga tgctcgccac agagcattaa
     5581 aaggcgtttg tggccttcgt gctccaggac ctccacttct ttccggatct gatccaggtt
     5641 tagcgctaca cgattgagtt ccttattgtc ccgcctgaag ccgcagtata gacagttgtt
     5701 ggaacagtga ttggccacat aaagcggcgc gaataaaacc agacggttgc cgtatatatc
     5761 ctgcttgatt ttatgcgccg cctgaaggat cgcgccgatc aggttttcat cttccgtctg
     5821 cagcagcaca gcgacttctt cgggtgtaag accttttagt tcgtatgctt tgtcgatcac
     5881 ctcgcggacg tgcaacgggt caggatttat cgtttgattc agaatattct gaatacgatc
     5941 gtcatcaata aaatctttta tcattttaaa tctgatattt tagtcttttc cggcagagcc
     6001 ttgttgatgg caacatgatt ttcataagga tcattttgtt aactctgttt ttcagcaatg
     6061 tgaacaatct ccttttccac ccagcgcggc gccatttcct tggcctcctc gtaggaaatt
     6121 ttcccgtgga ggatcatcgc cttgaaatca tcgaacatat agacgcccag aaacagatgg
     6181 acgcaaagaa acagcgtcaa cacccaggca aaaaatgtat gaagcgttcc cagataggcc
     6241 acggcggccc gcggggtcag gtgcgggaaa gcccacaaaa ccaatccgct gaggatcaga
     6301 accgtgccgc cgatgaaaac acagatgccg aacagtttct gtccggaatt gtattttccc
     6361 atggggggaa cggcgacttt tttatgaagg agcatctttt gcggatagcc gcccagtatg
     6421 gcgaaccacc gcacatcgtc catggaaaac gtgaagattc gttctatgaa aagaatcacc
     6481 cggtcgaatg ccagcagaaa aaaagccgcc gcgtccagcg tcaggacgac gccggcggcg
     6541 acatggatct gcatcgcaaa attcatactc tcttcagcca ggggcttgaa gtacaaaatg
     6601 acaccggtga cggccagcgg caggaaactt acgatcagca gataatgaaa cattctcacc
     6661 cgccaagggt gttttaaaat cattttttcg tgatgcatca tctatcctcc ttctaaatga
     6721 gacctttgga tcggttgatg tagccggtat gcagcagttc gtgggaaaga tggcccagcg
     6781 gctcgccgcc gaattcctta tagtagcgca tgacggactg gttggcgtgg ctgacgcgca
     6841 cggccgattt ttcatcatcc tgatacagcc ctttcgtgcg cgcctggata tactggttgt
     6901 tggacgttgc aatgcttttg aaccagacgg cgggcagcgc cagaaaagcg ataacaatgc
     6961 cgccgatggt aatgaattcg cggcgggtga catgcagcgg attttccatg tattgatatg
     7021 atttttcttc catttgattt ctcctttttt cctatctcca tgcaaaccag ggacggatgg
     7081 cgtcaatcca cgacgacccg tttccgtata tcggctggcc gccgccgttg atgcatccgc
     7141 cgggacagtt catgacttca ataaaatgat agggcgaacg tcccgccagg acttcatcca
     7201 cgacgggcgc aatattgcgc gcgattccgt gaacaacggc gacgttgatc gtaacgtcct
     7261 tgctgtattt actgtcgcgg atggtcaccg acgcgctttt cacgccgttg agcccccgga
     7321 cttttttgaa ttccagcggt ttcagctcgt tgccggtaat caccgcgtac gccgtgcgca
     7381 gcgccgcttc catgacgccg cccgtgttgc cgaaaatcgt gcccgcgccg gtataatcgg
     7441 ccagcagcgt cccgccttcc atctcttttt cggctgccag attgatgccg agctttttga
     7501 aaagtcgcga caggtcccgc gttgtcagca ccgtatccac atcgggatag gacgcgccgg
     7561 cggcctggcc ttttttgctg ttgtattcat aggcgctgat gaattcggga cgcgaggcct
     7621 ccagtttctt ggccgtgcag ggcatgatcc ccaccgtata aatcctgtca gccggtttgt
     7681 tccatacttt gactccgtat gtcttggcga cggttccggc catctgctgc ggagatttgg
     7741 ccgatgacaa atgcgggatc aacgccggat atttcgtttc gacaaaccgg acccaggccg
     7801 ggcagcagga ggtaaattgc ggcaacgggc cgatatgccc gccgtgcgcg ttttcctctc
     7861 cgaaaaccgc atggcgcgcg cgggcgatga attccgaccc ttcttccatg atggtcagat
     7921 ccgccgtgaa attgttgtcc agaatgtcaa atcccgcttt tctcatcgag ccgtagagtt
     7981 ttccggtcac gagacttccc gcgggaagcc caaaatcctc gccgatggcc acgcgcacgg
     8041 ccggagcgat gatgccgacg accgtggtat tcttatcctg caatttggcg ataaccgcgt
     8101 ccaccgtatc cgacgtttca taaggcgcgc cgaacggaca gttgatcaga cattgtccgc
     8161 aactcaggca caaatccgca ttgatgctgt gcgtcgcgcc gaacatgccc gtaatggcgc
     8221 ccgtcggaca atgtcgcttg caggcgtcgc atcccttgca aacggacgct ttgatggcga
     8281 tcacgccggg aatttcccct ttttgatatg tttttgcagc tacgttcatt ttccttttcc
     8341 tccgttatgt tttgacgctt gaaacggtcc ttatgtgcat ggatgcatat gtcaatgctg
     8401 gcatatgcta aaataagcat ataaagaatt cgtcaaactt tgtcaagttc ttaaagaaag
     8461 aatgttaaaa aaatggacaa aactaatttt gacgccctcg taaaaagtca gaattggtat
     8521 aacgctttaa aataatttag catttgtgca ttttttcctt gacggttttt ctctgaaatg
     8581 gtatgaatca tgttaaatat taatgagata ggtgttaacg ccatgattca tgcccgtgat
     8641 cacaaaacgc cctacttaa
//
LOCUS       DAOSIM010000064         8621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_7657_length_8621_cov_8.346253, whole genome shotgun sequence.
ACCESSION   DAOSIM010000064 DAOSIM010000000
VERSION     DAOSIM010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8621
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7657_length_8621_cov_8.346253"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /gene="carB"
                     /locus_tag="PLR47_04310"
     CDS             <1..715
                     /gene="carB"
                     /locus_tag="PLR47_04310"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418947.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HOG12196.1"
                     /translation="AIGVPLAKLATRVMLGKTLGELSFTKTVIPKHVCVKEAVFPFNR
                     FPGVDILLGPEMKSTGEVMGIDHSFGLAFAKSQMATGFKMPTSGSVFISVHDCHKAGI
                     ADVARSLADLGFNILATAGTAGYLNQRGIIATVVNKVSEGRPHVVDLIKNGDIQMVIN
                     TSVGRKSTQDAYLIRHGALSYNVIYTTTLAGARALGAAARALKEEGWGVCPLQEYYKK
                     QHLPLLPSLDTLKGTSGRN"
     gene            854..2281
                     /gene="purF"
                     /locus_tag="PLR47_04315"
     CDS             854..2281
                     /gene="purF"
                     /locus_tag="PLR47_04315"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOG12197.1"
                     /translation="MLPFVEGEQNDPGRPREACGLFAIYGNEDAARVTYFGLFALQHR
                     GQESAGVVVSNGCSVFEHKGMGLASTVFNENILQKLTGSLAIGHVRYSTTGSSTLSNA
                     QPFLVHVGDEYYALGHNGNIVNAQSLRSELEGRGSIFQSTMDSEVIVHLMAPHLKRGL
                     EKALCIALQQVEGAYSLVMLTRNRIVAARDPRGFRPLALGKLNGGWVVASETCAFDLV
                     GAEYIRDVEPGEILIIDADGCKSLKPFAPVKPCHCIFELIYFSRPDSQIFGQNVYLCR
                     KRLGHELAREYRPDVDLVMPFPDSGNYAAIGYAEESGIPFEMGMIRNHYVGRTFIQPT
                     QPMRDFGVRVKLNPVKPLLAGRKVMIVEDSVIRGTTSRNRVKNLREIGVKELHMVISC
                     PPTLFPCPYGIDFSSKGELIAAKKETEQAIAEFIGLDSLHYLTVDGMVRATGLSKDCF
                     CLACYTGDYPIAPPVKIDKYCMELN"
     gene            2357..2938
                     /locus_tag="PLR47_04320"
     CDS             2357..2938
                     /locus_tag="PLR47_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HOG12198.1"
                     /translation="METILSIAVGIGLAAACGFRVFVPLLILNLATLGGALTLPSGFA
                     WMGSHYATVGFSVATAAEVIGYYVPWFDHVLDTIATPAAIAAGTLTTAAFLTDVSPFF
                     KWTAALIAGGGTAGLIQASTVFLRAKSSLATGGAANPLFSTMENVSAVVTAILAILLP
                     LVCIVLMLIFCVWAARKVIRFFLKKNKVGTKSP"
     gene            complement(2996..3790)
                     /locus_tag="PLR47_04325"
     CDS             complement(2996..3790)
                     /locus_tag="PLR47_04325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG12199.1"
                     /translation="MPRKPMSYVRIIEDTFFAVCRYWGGLQNAFDVFGPVRAMQTGIA
                     SADLNMVWSESPLTQGDAPCVRQVEKYYETESLPYWWWIFPRAKTAETIRLLKDTGHV
                     HLLNMPCLLADLSALPKAKSCGPPATIREVGNQEDLALWQEVTFAGFDFPPGDKISHD
                     RFTGSFSLDPGSSQKAFLAFDGGRPVAASLLFLAGNAAGLYFITTLGEYRRRGIGRKL
                     VLATMHHARAAGARYASLQSSPDGLTVYEQAGFKEFCRVDIYGRKG"
     gene            complement(3780..4457)
                     /gene="pdxT"
                     /locus_tag="PLR47_04330"
     CDS             complement(3780..4457)
                     /gene="pdxT"
                     /locus_tag="PLR47_04330"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HOG12200.1"
                     /translation="MSVRLVGDEPGIIGVLDLQGGVHEHLEHLERLGLPYKRVKQASD
                     FADLAGLIIPGGESTCLARLLTIFDLKDVILQHHSRGMKIWGTCAGAILLATEVTGEK
                     PCLGLIDMEIERNGFGSQLDSFKSEAFIGAVSEEPVPLTFIRAPKILHAGEDVHVLLR
                     MDDYIAAVENEHILVTVFHPELTGSLAFHRYFALKCGLNPAAEKSSAADSGWKNVSWM
                     KLARIAS"
     gene            complement(4467..5348)
                     /gene="pdxS"
                     /locus_tag="PLR47_04335"
     CDS             complement(4467..5348)
                     /gene="pdxS"
                     /locus_tag="PLR47_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOG12201.1"
                     /translation="MDAKRYELNVQLAQMLKGGVIMDVTNVEQAKISEEAGAVAVMAL
                     ERVPADIRKDGGVARMSDPAMIAAIKKAVSIPVMAKARIGHFVEAQILEALRIDYIDE
                     SEVLTPADEECHIDKTKFSIPFVCGARNLGEALRRIAEGAAMIRTKGEAGTGNVVEAV
                     RHMRTMNREIRQVAQMPVEEVTGFAKTNGLPYELALKVKELGRLPVVNFAAGGIATPA
                     DAALMMQLGCDGVFVGSGIFKSADPARRARAIVKATAYFNDPQKVLEASMDLGEAMPG
                     LEIGQIPPEQILAKRGW"
     gene            complement(5470..6249)
                     /gene="hisF"
                     /locus_tag="PLR47_04340"
     CDS             complement(5470..6249)
                     /gene="hisF"
                     /locus_tag="PLR47_04340"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOG12202.1"
                     /translation="MISKRIIPCLDVRDGKLTKGIKFKGNVDIGDPVEAAREYYEQGA
                     DEIVFYDITASSDKRDIMIDVVRRVAETIFIPFSVGGGIRNLDDMRKVIGAGAEKVSV
                     NSAAVLEPRIISQGARAFGSQCVVLGMDVKKADVSGQTPSGYEIVIEGGRKHTGIDAL
                     EWAKEAERLGAGEICLNSIDADGMQDGYEINLTALISENVHIPVIASGGAGKPEHLAD
                     VLTAGRADAALVASMVHYRTYSILQIKKYLSDRHIKTRMLW"
     gene            complement(6246..6872)
                     /gene="hisH"
                     /locus_tag="PLR47_04345"
     CDS             complement(6246..6872)
                     /gene="hisH"
                     /locus_tag="PLR47_04345"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOG12203.1"
                     /translation="MIAIIEYNAGNITSVARALHSIGQDFVITDDTKMLNAASHAIFP
                     GVGAAGEAMAYLKDKKLDVWLKQCFRSGKPILGICLGTQIILDASEENDAACIGLVGG
                     TTRRFPESLTAAGRALKIPHMGWNSVAFRRPHPVFAGVPEEAEYYFVHSYYPSPESEE
                     AVLGTTEYGMTFCSVLAVRNLIAMQFHPEKSGRPGLQILKNFCAWEGK"
     gene            complement(6980..8299)
                     /locus_tag="PLR47_04350"
     CDS             complement(6980..8299)
                     /locus_tag="PLR47_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HOG12204.1"
                     /translation="MENLSDSKKNDVLPEFQTFLLEKKLVPDKHVFFYALWASKYFTY
                     ARKKQINSDQFQENAVIEFIEFLKADSNISDWQIRQAGDAIRLYYFHFRGLRPDNLST
                     IKAHDAIPELLKETQRLIRLKHYSYSTEKTYLQWIKRFLEYLQQTSKKDAFAEVDAAD
                     CKNFLSHLALNEKVAASTQNQAFNALLFLFRNVLCKEVGGLAQTVRAKRGQKLPVVFS
                     MEEVKRLLACLSGRDLLIAGLIYGAGLRLMELARLRVKDIDMDLNTLTVRSGKGDKDR
                     TTILPATVKNQLNNHLVEVKKMYENDLARGYGEAHLPDALSRKYPNAGKEWAWQYVFP
                     ANHLAVDPRSGKVRRHHISDTAIQDVIKKAIRKAGIPKFATVHTLRHSFATHLLMNGV
                     NIREVQELLGHKNVETTMIYTHVIRDMSHAPQSPLDTLLAGTKNQTG"
BASE COUNT         1935 a         2414 c         2226 g         2046 t
ORIGIN      
        1 ggccatcggc gtgccgctgg cgaagcttgc cacccgggtc atgctgggca aaaccttagg
       61 cgagctgtct tttacaaaaa ccgtcattcc caaacacgtc tgtgtgaaag aggcggtctt
      121 tccgttcaat cgctttcccg gcgtggacat actcctgggg ccggagatga aatccaccgg
      181 cgaggtgatg ggcatcgatc attccttcgg tctggccttt gccaagtcac agatggccac
      241 cggctttaaa atgccgacgt ccggcagcgt cttcatcagc gttcacgatt gccacaaagc
      301 ggggatcgcg gacgtagccc ggtcactggc cgatctgggt ttcaacattt tagcgacggc
      361 gggaacggcc ggatacctga accagcgggg catcatcgca accgtggtca acaaggtgag
      421 tgaggggcgt ccgcatgtgg tggatttgat caaaaacggc gacatccaaa tggtgatcaa
      481 caccagcgtt ggacgcaagt ccacgcaaga cgcgtatttg atccgtcacg gcgctttatc
      541 gtacaatgtc atttacacca cgacactggc cggcgcgcgg gccctcggcg cagcggccag
      601 ggcgttaaaa gaagagggct ggggggtctg cccccttcag gagtattaca aaaagcaaca
      661 ccttcctttg cttccctccc ttgataccct gaagggcaca agtgggagga attgaggttc
      721 accaagaggt tgaatgtttg gacgcaatca gaaccttttc ttccctccct tgatgggagg
      781 gattgaggga gggtgaaacg ccccctcccc ttcatcccct cccacaaggg gagggaaaat
      841 atggagaaac aaaatgcttc cctttgttga aggcgaacaa aatgatcccg gccggccccg
      901 cgaggcgtgc ggccttttcg ccatttacgg caatgaggat gccgcccgcg taacgtactt
      961 cggccttttt gccctgcagc atcgcggcca ggaatccgcc ggcgtcgtcg tctccaacgg
     1021 atgcagcgtt ttcgaacaca agggaatggg ccttgcctcc accgtcttca atgagaatat
     1081 tctgcaaaaa ctgaccggct ctctggccat cggccatgtt cgctactcca cgaccggttc
     1141 ctccaccctt tccaacgcgc agccgtttct ggtgcatgtg ggcgacgaat actacgcgct
     1201 ggggcacaac ggcaacatcg tgaacgccca gtccctgcgg tccgaactgg aaggccgggg
     1261 ctccattttc caatccacga tggacagtga agtcattgtt cacctgatgg cgccgcatct
     1321 gaaaagggga ttggaaaagg cgctttgcat cgcgcttcag caggttgaag gcgcctattc
     1381 tctggtgatg ctgacccgaa accggatcgt cgccgcccgc gacccgcgcg gttttcgccc
     1441 tctggcgctg ggaaaactga acggcggctg ggtggtggct tccgaaacct gcgccttcga
     1501 tctggtgggc gctgaatata tccgcgacgt tgaaccgggg gaaatcctga tcattgatgc
     1561 cgacgggtgc aagagcctga aacccttcgc gccggtgaag ccctgccact gcattttcga
     1621 gttgatttat ttttcccgtc ccgacagcca gatcttcggc cagaacgtgt atctctgccg
     1681 caagcggctg ggccacgaac tggccaggga ataccggccg gacgtcgatc tggtcatgcc
     1741 ttttccggat tccggaaact acgccgccat cggctacgcg gaggaaagcg gcattccctt
     1801 tgaaatggga atgatccgca atcattacgt ggggcgcacg ttcattcagc cgacgcagcc
     1861 catgcgcgat tttggcgtgc gcgtcaaact caatccagtc aagccgcttc tggccggccg
     1921 aaaagtcatg atcgtcgagg attccgtcat tcgcggcacc acgagccgca atcgcgtgaa
     1981 aaacctgcgg gaaatcggcg tgaaggaact gcacatggtc atcagttgtc cgccgacgct
     2041 tttcccctgt ccctacggga ttgatttttc ctccaagggg gaactgatcg ccgccaaaaa
     2101 ggaaaccgaa caggcgattg ctgaattcat cggcctggat tcgctgcact atctgaccgt
     2161 ggacggcatg gtccgggcca ccggcctgtc caaagactgc ttctgcctgg cctgctacac
     2221 cggcgactac cccatcgccc cgcccgtaaa aatcgacaag tactgcatgg aattgaattg
     2281 atttttcctt cgggaggctt caagctctcc cgaattcggc ttcccttttt ccgccacggg
     2341 gtgtaaaaag ccaggcatgg aaactattct ctccatcgcg gtgggaatcg ggctggccgc
     2401 ggcctgcggt tttcgagtct tcgtccctct gcttatcctc aatctggcga cgctcggcgg
     2461 agcgctgacc ctgccttccg gctttgcctg gatgggaagc cattacgcga cggttggttt
     2521 ttccgtcgcg accgcggccg aagtcatcgg ctactatgtt ccctggttcg atcatgtgct
     2581 ggacacgatt gcgacacccg cggcgattgc ggcgggaacc ctgaccacgg ccgccttcct
     2641 gacggacgtt tcaccgtttt tcaaatggac agcggctctg attgcgggcg gcggcacggc
     2701 aggcctgatt caggcttcga cagtcttttt gcgtgcaaaa tcttccctgg caaccggcgg
     2761 ggcagccaat cccctcttct ccacgatgga aaatgtgagc gcggtggtca cggcgatcct
     2821 ggccattctg ctgcctcttg tctgtattgt gcttatgctg atcttctgcg tgtgggccgc
     2881 aagaaaagtg atccgatttt ttctgaaaaa aaacaaagtc gggacgaaaa gcccctgatg
     2941 taaacttggg acgggcgcca cggaacgcag cgcctgaaac aaacgctttt gcctctcacc
     3001 ccttccggcc gtaaatgtcc acgcggcaga actctttaaa tccggcctgc tcatagacgg
     3061 tcaaaccatc cggcgaggat tgaagcgatg cataacgggc ccccgccgcc cgggcatgat
     3121 gcatcgtcgc caggaccagc tttcgaccaa tgccccgacg gcgatactcg ccaagcgtcg
     3181 tgatgaagta gaggcccgcg gcattacctg ccagaaaaag cagggaggca gccacgggtc
     3241 tgccgccgtc aaacgccaga aaggcttttt gtgaagagcc ggggtcgaga gaaaaggatc
     3301 cggtaaagcg atcatgcgaa atcttgtcgc cgggtggaaa atcaaaaccg gcaaaggtca
     3361 cctcttgcca cagcgccaga tcttcctgat tgccgacttc gcggatcgtt gcgggcggcc
     3421 cgcaggattt tgccttcggc aatgcgctga gatccgccag caggcaaggc atgttcagca
     3481 gatgcacgtg gcctgtgtct ttgagcaacc ggatcgtttc cgcggttttg gcgcggggaa
     3541 atatccacca ccagtagggc agggactccg tttcataata tttttcaacc tgccggacgc
     3601 atggcgcgtc tccctgtgtc aggggcgatt cgctccagac catgttcaga tcggccgagg
     3661 cgatccccgt ctgcatcgcc cggaccggac caaaaacgtc aaaggcgttt tgaaggccgc
     3721 cccaatagcg gcagacggca aaaaaagtat cttcaatgat gcggacataa gacatgggct
     3781 tacgaggcaa tgcgcgccag tttcatccag cttacgtttt tccagccgga atcggcggcg
     3841 gaagattttt cggcggcggg gttcaggccg catttcaaag caaaataccg gtgaaaggcc
     3901 agcgagccgg ttaactcggg atgaaaaacc gtaactaaaa tatgctcatt ttccactgcg
     3961 gcgatataat cgtccattcg gagcagaacg tgaacatctt ctcccgcgtg gagaattttc
     4021 ggcgcgcgga tgaaggttaa aggaaccggc tcctcggata cagctccaat aaacgcttca
     4081 cttttaaagc tgtcaagctg gctgccgaag ccgttgcgtt cgatttccat gtcgatcagg
     4141 cccaggcatg gtttttcgcc ggtcacttcc gtcgccagca gaatggcgcc ggcgcatgtg
     4201 ccccagatct tcatgccgcg gctgtgatgc tggagaataa cgtctttcag gtcaaagatg
     4261 gtcagaagcc gcgccagaca ggtgctttcg ccgccgggaa taatcaatcc tgcaagatcg
     4321 gcaaaatccg acgcctgttt gactcgctta tagggaagtc ccaatctttc cagatgttcc
     4381 aggtgctcgt gaacaccgcc ctgcaaatcc agaacaccga tgattcccgg ttcatcgccc
     4441 actaaacgca ctgacataac aaatacttac cagcctcgct tggccagaat ctgctccggc
     4501 ggaatctgcc cgatttccag gcccggcatg gcctcgccca gatccatcga cgcttccagg
     4561 actttttgcg gatcgttgaa ataggcggtc gccttgacga tggcgcgggc gcgtcgggcc
     4621 ggatcggcgg atttgaagat gccggagccc acaaaaacgc cgtcgcaacc gagctgcatc
     4681 atcagcgccg catcggcggg tgtcgcgatg ccgcccgcgg caaaattgac caccggcaga
     4741 cgtcccagtt ctttcacttt cagcgccagc tcatacggca ggccgttggt tttggcaaat
     4801 ccggtgactt cctctaccgg catttgcgcc acctggcgga tttcccggtt catggttctc
     4861 atgtggcgga cggcttccac aacgttgcct gttccggcct cgcccttggt gcggatcatg
     4921 gccgcgccct ccgcgatccg gcgcagggct tcacccaggt tgcgggcgcc gcagacaaac
     4981 ggtatcgaga attttgtctt gtcaatatgg cattcctcgt cggccggcgt caggacttcg
     5041 ctttcatcaa tgtagtcaat ccgcagggct tccagaatct gcgcttccac aaaatgaccg
     5101 attctggcct tggccatgac cggaatcgac acggccttct tgatcgcggc gatcatcgcc
     5161 ggatcggaca tgcgggccac gccgccgtcc ttgcggatat ccgcgggtac gcgctccagc
     5221 gccatcacag cgacggcgcc cgcctcttcc gaaattttgg cctgctccac attggtgaca
     5281 tccataatga cgccgccttt cagcatctgc gccaactgga catttaattc atatcgtttt
     5341 gcatccactt tttattcgcc tcctgacttt ctaaaaatat cctcttttgg gttcacaaaa
     5401 atttaaaata atttaatcat tggctattaa gaatacaaat ggaatttcgt caagtataat
     5461 aattggcggt taccagagca tccgggtctt gatatgacga tcgctcagat attttttaat
     5521 ctgcaaaatg gaataagtcc ggtagtgaac catggaggcg acgagcgccg cgtcggccct
     5581 gcccgcggtc agaacgtccg ccagatgctc gggtttgccc gcgccgccgg aggcaatcac
     5641 cggaatatga acattttccg aaataagcgc ggttaaattg atttcgtagc catcctgcat
     5701 cccgtcggcg tcaatggaat tgaggcagat ctctcccgcg cccagtcttt ccgcctcttt
     5761 ggcccactca agcgcgtcga tgccggtgtg ttttcttcct ccttcaatga cgatttcgta
     5821 tccggaggga gtttgtccgg aaacgtccgc ctttttcaca tccatcccca gcacaacgca
     5881 ctggcttcca aacgctcttg cgccttgaga gatgatccgg ggttcgagca ccgccgccga
     5941 attcacgctg actttttccg ctccggctcc gatgaccttg cgcatatcat ccagatttcg
     6001 aatgccgcct cccacggaaa agggaataaa aatggtttcg gccactcgcc gcacgacatc
     6061 gatcatgata tcgcgcttat cggatgaagc cgtgatgtca taaaagacaa tttcatccgc
     6121 gccctgctcg tagtattctc gcgccgcctc caccggatcg ccgatatcaa cattgccttt
     6181 aaatttaatg cctttggtga gcttcccgtc acgcacgtcc aggcagggaa tgattctttt
     6241 cgagatcatt ttccctccca cgcgcagaaa tttttcagaa tttgcaggcc cggccggccg
     6301 cttttttccg gatggaactg catggcaatc aggtttctca ccgccagcac cgagcagaag
     6361 gtcatgccgt attccgtcgt ccccaaaacc gcctcttcgc tttccggcga cggataatag
     6421 gaatgaacaa agtaatattc cgcctcttcc ggcactccgg caaacaccgg atgcggacgc
     6481 cggaaggcta cgctgttcca tcccatgtgg ggtattttca gagcccggcc ggccgcggtc
     6541 aggctttccg gaaaacgccg ggtggtcccg ccgaccaggc cgatgcacgc cgcgtcgttt
     6601 tcttcggagg cgtccagaat aatctgcgtg cccaggcaaa tgcccaagat cggctttccg
     6661 gatcggaaac actgtttgag ccacacatcc agctttttgt ccttgaggta agccatcgcc
     6721 tcgcccgccg cgccgacccc cggaaaaata gcatgggatg cagcattgag catttttgta
     6781 tcatccgtaa tgacaaaatc ctgtcctata gaatgcagcg cacgcgccac actggtaatg
     6841 tttccggcat tatattcgat aattgcaatc attctgctta atgtcctcga aaaatattaa
     6901 cgcattcata acattgctac tagcaaattt aatacctaaa cgccaagatc aatttgaatt
     6961 atgcttgcga taaagctgtt catcccgtct gatttttcgt cccagccagc aatgtatcaa
     7021 gcgggctttg cggcgcgtga gacatatctc ttatgacatg ggtataaatc atcgttgttt
     7081 ctacattctt gtgtccgagt aattcctgca cttcacggat attcacgcca ttcatcaata
     7141 aatgcgtcgc aaaactatga cgaagggtat ggacggtagc aaatttggga atcccggcct
     7201 tgcggattgc tttttttatg acatcttgaa tggccgtatc gctgatgtga tggcggcgca
     7261 ctttccctga gcgcggatca actgcgagat gattcgccgg gaaaacatat tgccatgccc
     7321 attccttgcc ggcgttagga tattttctgg acagcgcatc gggaagatgg gcttcgccat
     7381 atcctcgggc cagatcattt tcatacattt ttttcacttc gaccagatga ttatttaact
     7441 gattcttgac cgttgcaggc aggatggttg ttctgtcttt gtcacccttg ccactgcgca
     7501 ccgtcaatgt atttaaatcc atatcgatgt ctttcactcg caggcgggcc aattccatca
     7561 gccttagacc cgcgccatag attaatcctg caatgagcaa atccctgccg cttaaacagg
     7621 ccagcaatcg tttgacctct tccattgaaa agacaacggg cagtttttgc ccgcgctttg
     7681 cccggacagt ttgagcaagg ccacccacct ctttgcaaag cacattgcga aagagaaaaa
     7741 gcagggcatt gaacgcctga ttctgcgttg atgcggcaac tttctcattt aaagccagat
     7801 ggctgagaaa atttttgcaa tcggcggcgt caacttctgc gaaagcatcc tttttcgagg
     7861 tttgctgcaa gtattcgaga aaacgtttga tccattgcag ataagttttt tccgtgctgt
     7921 aggaataatg cttcagacgg atgagccgtt gcgtttcttt aagtagttca ggtatggcgt
     7981 catgggcttt tatcgttgat aaattatcag gtcttaaacc gcgaaaatga aaatagtata
     8041 agcggattgc gtcacctgcc tgccggattt gccagtcgga tatattggaa tctgctttca
     8101 gaaattcaat aaattcgatg actgcgtttt cctgaaattg atcagagttt atttgctttt
     8161 tgcgggcata ggtgaaatat ttacttgccc acaaggcata aaagaagaca tgcttgtccg
     8221 gcaccaattt cttctctaaa agaaaggtct gaaactctgg aagaacatca tttttcttac
     8281 tgtcggacag attttccata atataccaac ttggcttaag tgcgatatta ccacaaaata
     8341 ccaagcagaa aaagttcatt tagctggatt ttaaagcaat ttatttttgc attcttctta
     8401 aaaaagagta atattaaacc cgaaagtcgg aaaaaccaag ttggtatatt aattgttggg
     8461 ccacataacc tacagggata gtggaatgat tgatccgcaa attgaaggaa aagctgtttt
     8521 gataacaggg gcgaatcatg gcattggagc ggcaaccgcc agagcgtttg ctgttcaagg
     8581 cgcaaaactg taccttacct atttccggga accatgcgaa t
//
LOCUS       DAOSIM010000065         8590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_7693_length_8590_cov_7.268073, whole genome shotgun sequence.
ACCESSION   DAOSIM010000065 DAOSIM010000000
VERSION     DAOSIM010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8590
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7693_length_8590_cov_7.268073"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /gene="rfbA"
                     /locus_tag="PLR47_04355"
     CDS             complement(<1..713)
                     /gene="rfbA"
                     /locus_tag="PLR47_04355"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOG12205.1"
                     /translation="MAGIKKGIILAGGAGSRLYPLTLVASKQLQPVYDKPMIYYPLAT
                     LMTAGIQDILIISTPQDTPRFEALLGDGKRWGIRLYYKVQPEPKGIAQAFLVGEEFID
                     GDSVCLILGDNIFYGNMGLNKIVAGFQKGAVIFGYNVNDPERYGVVEFDKTGKAISIE
                     EKPARPKSNYAVPGLYLYDQKITKIARSMKPSARGELEITDVNMTYLNRGELHVVPLG
                     RGIAWLDTGTHTSLLEASH"
     gene            1146..2330
                     /locus_tag="PLR47_04360"
     CDS             1146..2330
                     /locus_tag="PLR47_04360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG12206.1"
                     /translation="MSDNRNPDLLIFEPQIAGHRLTWLQYITEDFLHAGYRVAWAIDS
                     RNGAKRAIQERLASAFDDIEAISVLDEKNGWRGGSKINAIAECLELSGAKHVFVNELD
                     EVASNIFRKAAVGVYPPENLKGRLSGVYFRPRFLTDPVWPPGNMIKAAGFRKLLEQEW
                     FENIYLVDEYLMASSRIRSAGHFHFLPDPWSGEYFCSSPNARRDIQIPADKFVFLHYG
                     IGDRRKGLHLAAAAMEKTPSDSRLFLLCAGQIDLEHSLLNHIAALEKKGKARLINRYV
                     SDEEERMIFAATDAVLLPYIHHYGSSGVLSRAAAAGKLVIASDEGLLARRVRDHGLGL
                     LFPTNDVPALQQRMTEAAALSREQRAGYQEKLLHYAGTCSRKNFRQALIAPWVRTGKT
                     DM"
     gene            2336..3460
                     /locus_tag="PLR47_04365"
     CDS             2336..3460
                     /locus_tag="PLR47_04365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOG12207.1"
                     /translation="MKNSPLLSKAPKKILLITTRRIGDVFLTAPLIASLREAYPDAKI
                     DMLVFKGTEGAVFANSALSRIVTVEEGAALPKQIKTVCRMFRKYDLALTTLTGDRPVF
                     YARIAGKKSVGFVENGIKHFWKRLLLNRYELFDNDDTHTVIMNLSLLKHLGIEPVCQL
                     AAVWTNADESAVRKLLPFDPAQKRYAVVHCYPMFPYKMWTFEGWKDIIFRLSDSLGLP
                     VILTGGNAADERDYISDLHRETAGRSIDVAGKISLGGVAFLLSKAAVYVGLDTAVTHM
                     AAALGVPTVALYGPTNPVKWGPMPGNHVFPGKSPYRMKGSQVVKNVFLLQGYGDCVPC
                     RKEGCERNIRSSSKCLQEIPADAVMQAVEERLAGNVKKMV"
     gene            complement(3624..4373)
                     /locus_tag="PLR47_04370"
     CDS             complement(3624..4373)
                     /locus_tag="PLR47_04370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG12208.1"
                     /translation="MQLSAIIITKNEEANIRTCLESIKWVDEIIVLDSGSTDATVAIC
                     REYTDKVHETDWIGFGPQKNRALNMAQGDWVLALDADEWVTPALKEEILRVVKDPGIY
                     VAFEIPRLSSYCGQYIHHSGWRPDYVTRLGRRGKVRFNDRLVHERMIVDGRTGRLSNS
                     IMHTSYKDLEQVLTKLNRYSAYGSELMFSKGKKSSLFKALSHGLWSFFYTYIARGGFL
                     DGRMGFILAVYNAECTYYKYLKLMMLDKDRR"
     gene            complement(4415..5269)
                     /gene="folD"
                     /locus_tag="PLR47_04375"
     CDS             complement(4415..5269)
                     /gene="folD"
                     /locus_tag="PLR47_04375"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOG12209.1"
                     /translation="MGNIIDGNKIAQEIRNEVRLKTLELRRQTGIIPGLAVILVGDDP
                     ASQVYVGRKAKACAEVGFLSREYKLPAETEEKKLLKIIKKLNKDDQIHGILVQLPLPG
                     HISTENIIAAIDPDKDVDGFHPYNVGGLMTGTPLFVPCTPRGIMELIARSGIDLKGKE
                     AVVVGRSNIVGKPMAMLLLAQHATVTMCHSRTGDLPAVTRRADVLIAAVGKPHMIKAD
                     MVKEGAVVIDVGVNRLENGKLAGDVAFDDVAPKASFITPVPGGVGPMTIAMLMRNTLD
                     SAARCQRK"
     gene            complement(5506..6051)
                     /locus_tag="PLR47_04380"
     CDS             complement(5506..6051)
                     /locus_tag="PLR47_04380"
                     /inference="COORDINATES: protein motif:HMM:NF021231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HOG12210.1"
                     /translation="MKKIIVALLLVLSLGVFTSYAATLKVGDKLGDFKLNDALDNKAY
                     NLASPEFAGKVVYIVYASSSSADDNDHVTAALNANQDIMNLKSANKYVGLGIGNQKDS
                     PVPNFIIKQISKRKQKSSGAIILLDPDFTIGKVLGLASPNKGAVSVLVDKNRIVRYIY
                     SGKTPAANIPKIVELIKQYSE"
     gene            complement(6276..7181)
                     /locus_tag="PLR47_04385"
     CDS             complement(6276..7181)
                     /locus_tag="PLR47_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HOG12211.1"
                     /translation="MKVFMTGGTGFVGTSLAKRFISEGHSVTILTHDNASATLKMDGL
                     DYLEGNPTVAGNWQTKVPAYDVIVNLAGASIFSRWTDRQKNILLSSRILTTRNLVDAL
                     PKDAGYITFFSTSAVGYYGFHQDEELTEASPAGADFLARLADDWEREALKAQQKGARV
                     IITRFGIVLGKNGGALGQMIPLFKFFLGGPLGSGRQWFSWIHMRDLAEAFIFLLAKKD
                     ISGPVNICSPQPVRNLELGHAIGRVLHRPSFLPAPGFMIKLILGEFGSVLLEGQRVIP
                     KRLLDAGYKFTHPGIDQALKDIIVT"
     gene            complement(7242..7625)
                     /locus_tag="PLR47_04390"
     CDS             complement(7242..7625)
                     /locus_tag="PLR47_04390"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOG12212.1"
                     /translation="MTLSEYFEKAKGVGVLSTADKNGKVNAAIYGRPHFMDEKTLAFI
                     AADRLTHANLKANPSAVYLFKENGSYEGKRLYLAMTHEEKDSPLIENLRRKKRKEYDS
                     TDKKESKFLVYFKVKKVLPLIGAGA"
     gene            7696..8214
                     /locus_tag="PLR47_04395"
     CDS             7696..8214
                     /locus_tag="PLR47_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOG12213.1"
                     /translation="MMRKRFLIGLVFLLAGCVGVPDGVKPVEKFQLERYLGKWYEIAR
                     LDHSFERGLSQVSAEYSLNADGSVKVINRGFSDKDKKWKEAVGKAYFVKRPDQGFLKV
                     SFFGPFYGSYIILELDHKNYAHALVCGPNRSYLWILARSPKISDEVKNSLVAKAAALG
                     FQTGNLIYPRHD"
BASE COUNT         2009 a         2179 c         2245 g         2157 t
ORIGIN      
        1 aaatggctgg cctccagaag gctggtgtgc gttccggtat cgagccaggc gatgccgcgt
       61 cccagcggaa caacatgaag ttcgccgcga ttcaagtacg tcatgttgac atctgtgatc
      121 tccagttcgc cgcgggcgga cggtttcatg gacctggcga ttttggtaat tttctgatcg
      181 taaagataaa gcccgggaac ggcgtaattg gatttgggtc tggcgggttt ttcttcaatg
      241 ctgatggctt tgccggtttt gtcgaattcc acgacgccgt agcgctccgg atcattgacg
      301 ttgtacccga agataacggc gcctttctga aaacccgcaa cgattttatt cagtcccatg
      361 ttcccgtaaa aaatattatc gccgagaatc aggcacacgg aatcgccgtc gataaattcc
      421 tcgccgacca gaaaggcctg ggcaatgccc ttgggctcgg gctgcacttt gtagtaaagt
      481 cggatgcccc agcgtttgcc gtcgcccaac agggcttcga accgcggcgt gtcctgcggc
      541 gtggagatga tgagaatgtc ctgaatgccc gccgtcatga gcgttgccag cggatagtaa
      601 atcatcggtt tgtcatacac cggctgcaac tgtttgctgg ccaccagggt caggggataa
      661 agcctgctgc ccgcgccgcc ggccagaatg atgccttttt tgatgcctgc cattttgtta
      721 cctcgccatt ggttgatggt tgatggttaa aaaatagttt taagttttac gtgttatgtt
      781 ttacgcagat aacgtaaaaa gcgaaacacc gctgtcattc ccgcatgcgc gggctattta
      841 cagctccgcc ctctcagtta catcggcaag ctcgatataa cctccggaaa aagattaaat
      901 atgtaatcaa attgatatta taatgtcaat tgctaagttc ccgtagcggt gggcgctccg
      961 gcggaaatta cctgtttata ttcaaaatta tttatgttaa atgcccggat atacatgacg
     1021 gcgcctgtat tactttttta aatcaattga tttcccggtg acggtatatt caggaccgtc
     1081 gtaaattgaa atatttctca cccgtcctgt ttgcagaatg ccgctgacgg gaaaaagagc
     1141 atgatatgtc tgacaaccga aatccggatc tgctgatatt cgagccgcaa attgccgggc
     1201 accgcctgac gtggcttcag tatattaccg aagattttct tcacgcggga taccgcgttg
     1261 cctgggcgat cgactcgcga aacggcgcga aacgcgcgat tcaggaacgt ctcgcttccg
     1321 cgtttgacga tattgaggcg atttctgttt tggatgagaa aaacggctgg cggggcggca
     1381 gtaagatcaa tgccatcgcg gaatgtctcg agctcagcgg ggctaaacat gtttttgtca
     1441 acgaactcga tgaggtggcc tccaatatct tccgaaaagc ggcagtgggc gtttacccgc
     1501 cggaaaatct caagggcaga ctcagcgggg tttatttccg tccacgcttt ttgaccgacc
     1561 cggtctggcc gccgggcaac atgatcaagg ccgccggctt ccggaaactc ctggagcagg
     1621 aatggtttga aaatatttat ctggtggatg agtatctgat ggcgtcttcc cggatacggt
     1681 ctgccggcca ttttcatttt ttgccggatc cctggtcggg agagtatttt tgttcttctc
     1741 caaacgcaag acgagatatc cagattcccg cagacaagtt tgtgtttctg cactacggca
     1801 tcggagaccg gcgcaaaggc ctgcatctgg ccgcggcggc catggagaaa acgccctccg
     1861 actcccgcct gtttttatta tgcgccggac agattgatct ggaacattcc ctgcttaacc
     1921 atatcgccgc actggagaaa aaagggaagg cccgactgat caatcgctat gtgtccgatg
     1981 aggaagaacg catgattttc gccgcaacgg acgcggtttt gctgccctat attcatcatt
     2041 acggatcaag cggcgtcctg tccagagcgg cggccgccgg gaaattggtg atcgcctccg
     2101 acgaaggcct gctggccagg cgtgtgcggg atcatggcct gggcctgctt tttccgacca
     2161 acgatgttcc cgccctgcag cagcgcatga cggaagcggc ggcgttgagc cgcgagcagc
     2221 gagccgggta tcaggaaaag ctgctgcatt atgccgggac gtgttcgcgg aaaaattttc
     2281 gtcaggcatt gattgcgccc tgggtccgta cgggaaagac agatatgtaa aaacgatgaa
     2341 gaacagtccc ttgttatcaa aagcgccgaa aaaaattctg ttaatcacga ccagacgcat
     2401 cggcgacgtt tttctgacgg cgccgctgat tgcctcgttg cgcgaggcct acccggatgc
     2461 caaaatcgat atgctggttt tcaaaggaac ggaaggtgct gtttttgcaa acagcgcgct
     2521 cagcaggatt gtcacggtag aggaaggcgc cgcgctcccg aaacaaatca aaaccgtttg
     2581 ccggatgttc aggaaatacg atctggcctt aacgacgctt acgggagacc ggccggtttt
     2641 ctatgcccgc atcgccggaa aaaaatcggt cgggtttgtg gaaaacggaa taaagcattt
     2701 ctggaaacgg ctgcttttga accgctatga gttgttcgat aacgatgaca cgcataccgt
     2761 cattatgaat ctttctcttc taaagcatct ggggatcgag ccggtttgtc aactcgccgc
     2821 tgtgtggacc aacgcggatg aatcggcggt cagaaaactt ctgcccttcg atccggcgca
     2881 aaaacggtac gccgtggttc attgttatcc catgtttccc tataagatgt ggacgttcga
     2941 gggatggaaa gatatcattt tccggctgag cgattcgctg gggctgccgg tgatactaac
     3001 cggcggcaat gccgcagatg aacgtgacta tatttccgac cttcatcggg aaaccgccgg
     3061 cagaagtata gatgtcgccg gcaaaatcag cctgggcggc gttgcttttt tgttgagcaa
     3121 agccgccgtc tatgttggtc tcgatacggc ggtcacgcat atggccgcgg cactgggcgt
     3181 tccgacggtc gcgctctacg ggccgacgaa tcccgtcaaa tggggaccga tgcccggcaa
     3241 tcatgtcttc cccggcaaga gtccttaccg catgaaagga tctcaggttg tcaaaaatgt
     3301 ttttcttttg caggggtatg gggattgcgt tccgtgccgc aaggaagggt gcgaaaggaa
     3361 catccgaagt tcaagcaaat gccttcagga aataccggcg gatgcggtga tgcaggcggt
     3421 ggaagaaagg ctggccggca acgtcaaaaa aatggtttga gacgttgtgc tccaaaataa
     3481 gcgataactc cctatggcac aatccctctt ttgtcatttc aggggacaaa gcgttgcgaa
     3541 attttagagc cttttaacat tgaacgtaga acattgacgg ttgaacttct taaccatcaa
     3601 ccatgaaccg gtgtcgcaac aacttatcgc ctgtccttat ccaacatcat cagttttaaa
     3661 tatttgtagt aggtgcattc cgcattgtaa acggcaagga taaatcccat gcggccgtcc
     3721 agaaaaccgc cgcgggctat gtaggtgtaa aagaaactcc acaaaccgtg ggagagggct
     3781 ttgaataagg aggatttttt gcccttggaa aacatcagtt cagagccgta ggccgaatac
     3841 cggttgagtt tggtcagaac ctgctcgagg tccttgtagg aggtgtgcat gatgctgttg
     3901 gataaacggc cggtccgtcc gtcgacaatc atcctctcat gaaccagccg gtcattgaac
     3961 cggaccttgc ccctccggcc gagacgggtc acgtaatcgg ggcgccagcc gctgtgatga
     4021 atatactgcc cgcagtaact cgacaaccgg gggatctcaa acgcgacata aatgccggga
     4081 tccttgacga cccgcagaat ctcttccttg agcgcgggcg ttacccattc gtcggcgtcc
     4141 agcgccagca cccaatcgcc ctgcgccatg ttcagggccc ggtttttttg aggcccgaaa
     4201 ccgatccagt cggtctcatg aaccttgtcg gtatattccc ggcagatggc cacagtggca
     4261 tcggtgctgc ccgaatccag aacgatgatt tcgtcaaccc acttgatcga ctccagacac
     4321 gtccggatat tggcttcttc gtttttggtg atgatgatgg ctgatagttg cataccggca
     4381 ttttgtgaga aactatggtt tcaacttttc ttttctattt tcgttgacat cgcgcagccg
     4441 aatccagggt gtttcgcatc agcatggcaa tggtcatcgg cccgacgccg ccgggaaccg
     4501 gcgtgatgaa agatgccttg ggcgccacgt cgtcgaaagc cacgtctccg gctagtttgc
     4561 cgttttccaa acggttgacg ccgacatcga tgacaacggc gccttctttc accatatcgg
     4621 ctttgatcat gtgcggcttg ccgacggcgg cgatcaggac atcggcgcgc cgggtcacgg
     4681 ccggcagatc tccggttctg gaatgacaca ttgtcaccgt cgcatgctgc gccagaagca
     4741 gcatcgccat gggtttgccg acaatgttgc tgcgcccgac aacgacggct tccttcccct
     4801 tgagatcgat tccggaacgg gcaatcaatt ccatgatccc gcggggcgtg cagggaacaa
     4861 aaagcggcgt gccggtcatc aggccgccga cgttgtacgg atgaaagcca tccacgtcct
     4921 tgtcgggatc gatggcggca atgatgtttt ccgtggaaat atgtccgggc aggggaagtt
     4981 gtaccagaat gccgtgaatc tggtcatcct tgttcagctt tttgatgatt ttcagcagct
     5041 ttttttcttc cgtctccgcg ggcagtttgt attcacggga aaggaaaccg acttccgcac
     5101 aggccttggc ctttctgccg acatacacct gcgatgcggg atcgtctccg acgagtatga
     5161 cggccagtcc gggaatgata cccgtctgcc gccggagctc cagggttttc agccgcacct
     5221 cgttgcggat ttcctgtgcg attttattgc catcgataat gttgcccatg aacggtgtct
     5281 cctctatgac ctacagaagc aaacgctgac cggatatcca tcaggctgcc ttccaccctg
     5341 ccaaatgccg tgtcatgatg atgccgggcg accggcacgg atgttgcgct gtaatcatgc
     5401 gttttggtaa ttcaggttat atgattcgct tgcagaaccg gaatcgatct cccccggatc
     5461 gcgcgaagcg tccgggggag aaatgacttt taagcgtcag atcctttatt cagaatattg
     5521 tttaatcaac tcgacaattt tcggaatatt tgccgccggt gtctttccgg aataaatgta
     5581 acggacgatt ctgtttttat ccaccaggac ggaaacggcg cctttgttcg gcgacgccag
     5641 ccccaggacc ttgccgatgg tgaaatcggg atccaggaga ataatggcgc cgctgctttt
     5701 ctgtttgcgc ttggaaatct gcttgatgat aaagttcggc acgggactgt ccttctgatt
     5761 gccgatgccc agaccgacat acttatttgc gctcttcagg ttcatgatgt cctggttggc
     5821 gttgagcgcg gcggtaacat gatcattatc gtccgcagag cttgagctgg catatacgat
     5881 gtaaacgact ttgccggcaa attccggcga ggcgagattg taggctttat tatccaacgc
     5941 atcgttaagt ttgaaatcac ctaatttgtc acccactttc aacgtggcgg cataagacgt
     6001 aaaaaccccc aacgacaaca ccagcaataa agcgacgata atttttttca tttcctcctc
     6061 ctttttaatc cccacataat gattcctttt ggcaacataa aaatatgtaa gttatggaga
     6121 ttttaaaaat ttggcgcaat tatagagtaa aaaaatagca cgggcaagca aaaagaaata
     6181 tttgtccaag gggctgttat tatttgaatt aacgtttccc aaacacggct tgccgttttc
     6241 gggaaagttc agcgccggcc cgaagggcat ccgtctcagg tcacgatgat gtctttcagc
     6301 gcctgatcaa ttcccggatg tgtaaatttg tatccggcat ccagcagacg tttcgggatt
     6361 actctctggc cttcgagcag aacggaaccg aattcgccca gaatcagttt aatcataaag
     6421 ccgggcgccg gcagaaatga cgggcggtgc agcacccgtc cgatggcatg gcccagttcc
     6481 aggttgcgca ccggctgcgg agagcagata ttcaccggtc cggagatgtc tttttttgcc
     6541 agcagaaaaa taaaggcttc ggccagatcg cgcatgtgaa tccaggaaaa ccactggcgg
     6601 ccgctgccca gcggacctcc cagaaaaaat ttaaacagcg gaatcatctg ccccagcgcg
     6661 ccgccgtttt tccccagcac gatgccgaag cgggtgatga tcacgcgggc gcctttttgc
     6721 tgcgccttca gggcttcgcg ttcccagtca tcggccagcc gcgcgagaaa atcggcgccg
     6781 gcgggcgacg cttccgtcag ttcttcatcc tgatgaaaac cgtaataacc gacggcggag
     6841 gtgctgaaaa acgtgatgta cccggcgtct ttcggcaacg cgtccaccag attccgggtg
     6901 gtcaagatgc ggctggagag cagaatgttt ttctgccggt ccgtccagcg gctgaagatg
     6961 gacgcgccgg caagattgac gatcacatcg taggcgggca ctttggtctg ccagtttccc
     7021 gccacggtgg ggttgccttc cagatagtcc aggccgtcca tttttaatgt cgccgatgcg
     7081 ttgtcgtgcg tcaggatggt taccgaatgg ccttcggaaa taaaacgctt cgccagagac
     7141 gtcccgacaa accccgtgcc gccggtcatg aatactttca tcacagccct cccggaaatc
     7201 aatgctttac gattttattg agttcaatca tgcgttgacg tttatgcgcc ggcgccgatc
     7261 agcggcagta cttttttcac tttgaaatag acgagaaatt tcgactcttt tttatcggtc
     7321 gagtcgtatt ctttccgctt tttgcggcga agattttcaa tcaaaggcga atccttttct
     7381 tcgtgcgtca tcgcgagata aagtcttttg ccctcatacg atccgttctc tttaaaaaga
     7441 taaacggcag aaggattggc cttcaggttg gcatgggtga gacgatcagc ggcaatgaag
     7501 gccagcgttt tctcatccat aaaatgaggc ctgccgtaaa tcgccgcatt gactttcccg
     7561 tttttgtccg ccgtggaaag gacaccgact ccttttgctt tttcaaaata ttccgacagc
     7621 gtcataattc ctcccttgat tttaatcttc tttggggtaa attaagtcat cctcgtggaa
     7681 ttgtaaagga gataaatgat gcgtaaaaga ttcctgatcg gtttggtttt tttgctggcg
     7741 ggctgcgtcg gcgtaccgga cggtgtcaag ccggtggaga aatttcagct cgaaaggtat
     7801 ctgggcaaat ggtacgaaat cgcaaggctc gatcactcct tcgaacgcgg gctctcgcag
     7861 gtctccgccg aatacagcct gaatgccgac ggaagcgtca aggtgattaa ccgcggattt
     7921 tccgacaagg ataaaaaatg gaaagaagcc gtcggcaaag cgtattttgt gaaacgtccc
     7981 gatcagggtt ttctgaaagt ctcctttttc ggcccttttt acggctcgta tattattctg
     8041 gagctggatc ataaaaacta tgcccacgcg ctggtctgcg gtccgaaccg ctcctattta
     8101 tggattcttg cgcgttcgcc caaaatcagc gacgaggtga aaaattccct ggtcgccaaa
     8161 gccgccgcgc tgggttttca gacgggaaac ctcatttacc cgcgacacga ctaaaaaggg
     8221 cattaaatct gcaagcaatt gatattgata agaatttata atttatggtg taaattctgt
     8281 gtgacctttc ggcaactttt cccttttgac cccaacaccg caataatcag catctaacat
     8341 attgttatta aaagattatt ttattttttc cggatttttt ggcgccttcg ggttgtaatc
     8401 ctgcatcaaa tgtgctaaaa aaacatacat tgaagtcaaa gagactttct taatttttcg
     8461 taccaccaga ttaacctcgt cgttcaataa cagcggcaaa ccaaaaataa taaacatcag
     8521 gagacggatt tgaagaatga ttcccatcat attttatttc gcttgcctga aaaaaaagac
     8581 gcgcaggcgg
//
LOCUS       DAOSIM010000066         8514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_7775_length_8514_cov_7.727864, whole genome shotgun sequence.
ACCESSION   DAOSIM010000066 DAOSIM010000000
VERSION     DAOSIM010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7775_length_8514_cov_7.727864"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..1844)
                     /locus_tag="PLR47_04400"
     CDS             complement(156..1844)
                     /locus_tag="PLR47_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG12214.1"
                     /translation="MGNVYLERPWLKNYDKHVPHKLKYEEKSFTELFSEVVSRYPDKT
                     ALIYLGRSITFREVDILSNQIAHFLIRAGLKPDDVVGLHLPNLPAHYIAVLGAQKAGC
                     ITTGLSPLLTAHEMAHQLKDSDTKVIFTVDVLYSKVAEVAGKAPFSTVVVSEIADFLP
                     GVKRVLGKLLKKIPTAEVKPIAGKTVLRFMEIMKEMPKDPVAVRRSFDDTIFMMYTGG
                     TTGPAKGALLTQRSYMSNRQQMLAWVDLHAEDIALSAFPLFHIAGLALGGFSLTHGIT
                     QICVPNPRDTHFLIEALKKYRPTFVVNVPTVFFELLKKPDFKALDLKGHLKWCMSAAA
                     PFPAENIKELESIIGEGNFIELYGMTEMSPVMICNPLKGRKKPASVGMPFPDTEVQLL
                     DTATGNPVAPGEPGEIVIRGPQLMRGYFNKPEETANAVRDGWMHTGDVAKMDEDGYFY
                     LVDRVKDMVIVSGFKVFTRELDEILVTHRDVQMAASIGLADPERPGSERVGCAIVLHE
                     GVEKTDAKKEEIRAFLKERIAPYKMPRVIEFMDELPVSGVGKVLKRELKKMMTV"
     gene            complement(2056..3114)
                     /locus_tag="PLR47_04405"
     CDS             complement(2056..3114)
                     /locus_tag="PLR47_04405"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF002699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HOG12215.1"
                     /translation="MKEFMKKKDLLKKPVEHIDIKKINAADIIRAMKKMSFSARDLAS
                     AAELYDRMLREKNCAVILTLAGSTSAAGCMQVYVDLVKNNMVDVIVATGASIVDMDFF
                     EALGFRHYQGTPFVDDKLLRSLYIDRIYDTFISEDDLQHCDQTIKKIADSLEPRPYSS
                     REFIAEMGRYLTKNAKKKNSLVQAAYENDVPIFCPAFSDSSAGFGLVLHQVEHPDNHL
                     SIDSVKDFRELTEIKIKAGTTGLLMIGGGVPKNFAQDTVVCAEILGHDVTMHKYAVQI
                     TVADVRDGACSSSTLKEANSWGKVDSSCEQMVYAEATTALPLLASYAYHKGSWKKRKK
                     RALSRIFAGDSGSRKKKL"
     gene            complement(3202..4734)
                     /locus_tag="PLR47_04410"
     CDS             complement(3202..4734)
                     /locus_tag="PLR47_04410"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG12216.1"
                     /translation="MTLVKIRKVISGIIFVLFVLLFLGPEKWSVFLSDVIAPFQFVPS
                     LVQTATAPSGLFMAGFIFIVLMTLIFGRVYCSFLCPLGTLQDLIIAGSRRLGLLPGHA
                     FAGPHNGLRYALLASTAAAAVIGSLSLVSLLDPYSLAGRFFSHFLQPLVVGAYNLGIA
                     ALKPFDVYWYPKQTAFVPLSVLAATAAFFLLILYLAATRGRLYCNTVCPVGTFLGLVA
                     RFSVFQLTVNPKACSECVRCENVCKAGCIDSHNAFVDMSRCIGCLNCLDACSQSAIRY
                     RRRQRQPHAGAWSPSRRGFVMGGIAAAAALLALNTKLRAWIDIRRAQAEPPVTPPGSR
                     DIAHFTRACTACSLCVSVCPTQVLTPAFMDFGASGLMQPKMNYEKSFCDYECSLCSRV
                     CPTGALASLSLEEKKLTQIGEAELLENICVVYVDHNNCGACGEVCPTHAIRFADKKNI
                     LYPEIDRQYCIGCGACQLACPTAPRSIVVHANPVHKKAEKYISPETTVGQKKPADDEF
                     PF"
     gene            complement(4747..5700)
                     /locus_tag="PLR47_04415"
     CDS             complement(4747..5700)
                     /locus_tag="PLR47_04415"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOG12217.1"
                     /translation="MADATKFNRREILQKGLSLGLIAGGAVILGRPAFLSAKEKPAVI
                     PDLVAVKNGEPDALFKKAIELAGGMNQFVKKGQTVVVKPNIGFPRVPEVGATTNPLLI
                     KTIIESCWAAGARRVYVFDNVVTPTSGNARNCYKLSGIEEAAKSARGIIVPVDDFSYR
                     TVKIPGGKTLKTADVHSLILDCDVFINVPVLKHHSSAHLSIAMKNLMGIVKNRMEYHL
                     TGLDQCIADFCLYRRPDLNVVDAYRVLMSRGPAGPGNPRAADVQMKKTLLISKDIVAV
                     DAAAARIFGKDPEDIGYIKIAHAQKTGNMNLKELKIVTYTM"
     gene            5877..6371
                     /locus_tag="PLR47_04420"
     CDS             5877..6371
                     /locus_tag="PLR47_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12218.1"
                     /translation="MQNPTISATGVASFRRAATAFGLLVFLPCLLSLINVTFAQSWKI
                     HFFPAAVFLAAVGFGPAGGLIAGITGSLYSAVALGNPWLIAGNALLGFLTGFFFRRTS
                     KIILSVLLAFAVQLPWLILSDYYFAGLSPLFIAKLVMVLFLGNLLWALLISLGMKPIK
                     KYLC"
     gene            6365..6772
                     /locus_tag="PLR47_04425"
     CDS             6365..6772
                     /locus_tag="PLR47_04425"
                     /inference="COORDINATES: protein motif:HMM:NF020543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1893 domain-containing protein"
                     /protein_id="HOG12219.1"
                     /translation="MLTPDFTRYSLALLEGDRLIHSSKGGGLRPLWDALEKYSEKSGL
                     ILHDKVMGLAAARLIHRSGIIAEVHTMVVSLPAVQFLKDHGIRLTAFHSAANILTRDK
                     SAVCPGEAIALGTGEPDAFYQKIREMMEIPVIA"
     gene            complement(6825..8303)
                     /locus_tag="PLR47_04430"
     CDS             complement(6825..8303)
                     /locus_tag="PLR47_04430"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG12220.1"
                     /translation="MQEHNPEEGMEKSEVAGLGEICLEDLNPELKTACARAGWPSLVP
                     VQARTIPYLLAGRDVMIQSRTGSGKTGAYLLPIIQKINLAKNVAQALVLVPTRELALQ
                     VYRDSQVLAEGTGLKTAVVYGGVGYGPQLEAFRSGAQLVVGTPGRILDHLLKNTLTLD
                     HLRMLIFDEADRMLSMGFYPDMVKIRKYIPTGKIASSMFSATFPPQVIRLAGQFLREP
                     ELLSLSGNEVHIAESEHIYYVVPGMRKERSLVRIIEIENPASAIIFCNTKDTVHYLSV
                     VLKRFGYDADELSSDLAQTAREKVMARVRRGALRFLVATDVAARGIDIPDLSHVFQYE
                     PPEDPEAYIHRAGRTGRMGSSGVVISLVAEMERFKIQSIARFYTINMEERSLPGDEEL
                     ERVVSERITALLEARLRGRDNIQIERMQRFKPLARNLAQSEEELAIITMLLDDYYQQS
                     LHAPPVQPAADDAEAPGPTVQRPKPQRPNKRPRPGKRKEINP"
BASE COUNT         1800 a         2411 c         2389 g         1914 t
ORIGIN      
        1 tcgatcaggc catcgagttc atcgccgaag acgaactggt ggaagtgacc ccgcaaagcg
       61 ttcgcctgcg caagatggag ctggacgcga cgaagcgtca gatgaaagcc cgccgggagg
      121 agtaggcccg cgggcttgcg ccgccgcgag cggtcctaca ccgtcatcat ctttttcaat
      181 tcgcgcttga gcaccttgcc cacgccgctt acgggaagct cgtccatgaa ctcgatgacg
      241 cgcggcatct tgtagggcgc tatccgttcc ttgagaaagg cgcggatctc ttcctttttc
      301 gcatcggttt tttccacgcc ttcatgcaga acgatggcgc agccgacgcg ctccgagccc
      361 ggacgctccg gatcggccag gccgatgcta gccgccatct gcacatcccg gtgcgtcacc
      421 agaatctcgt ccagctcccg ggtgaaaacc ttgaatccgg acacgatcac catgtctttt
      481 acccggtcca ccaggtaaaa ataaccgtcc tcgtccattt tcgccacgtc cccggtatgc
      541 atccacccgt cccgaacggc gttggcggtt tcttcgggct tgttgaaata acccctcatg
      601 agctgcggcc cgcggatgac gatctcgccc ggttcgcccg gcgctacggg gttcccggtc
      661 gccgtatcca ggagttgaac ttcggtgtcg ggaaacggca tgccgacgga cgccggtttt
      721 ttcctgccct tgaggggatt gcagatcatc accggcgaca tctccgtcat cccgtagagt
      781 tcgatgaaat ttccctcacc gataatcgat tccagctctt tgatgttctc cgccggaaac
      841 ggcgcggctg cgctcatgca ccacttgagg tgtcccttga gatcgagcgc tttgaaatcg
      901 ggctttttca acaattcaaa aaagaccgtg ggaacgttca cgacaaaggt cggacggtat
      961 tttttcagcg cttcgatcag gaagtgcgta tcgcggggat tgggcacgca gatctgggtg
     1021 attccatgcg tgagcgaaaa accgcccagg gcaagccccg cgatatgaaa cagaggaaat
     1081 gccgaaaggg caatgtcttc ggcatgaaga tcgacccacg ccagcatctg ctgacgattg
     1141 ctcatgtagc ttcgctgggt aagcagggcg cccttggccg ggccggtggt gcctcccgtg
     1201 tacatcatga agatggtgtc gtcgaacgat cgcctgaccg ccacgggatc tttgggcatt
     1261 tctttcatga tctccataaa gcgcaaaacg gtttttccgg cgatgggctt cacctccgcc
     1321 gtggggatct ttttcaaaag cttccccagc acgcgtttca cgcccggcag aaagtcggca
     1381 atttccgaca cgaccaccgt tgaaaaaggg gcttttccgg ccacttccgc caccttggaa
     1441 tagagcacgt ccaccgtgaa gatgactttc gtgtccgaat ctttaagctg gtgcgccatc
     1501 tcatgcgccg tcaggagcgg agacaggccc gtggtaatgc atccggcctt ttgcgccccc
     1561 agcacggcaa tatagtgcgc cggaagattg ggcagatgca ggccgacgac atcgtccggt
     1621 ttcaggccgg cccggatcag aaaatgggca atctggttgg agaggatatc cacttccctg
     1681 aaggtgatcg atcgccccag gtaaatcaaa gcggtcttgt ccggataccg ggagaccact
     1741 tccgagaata actcggtgaa gcttttctcc tcgtacttca gcttgtgcgg cacatgcttg
     1801 tcgtaatttt tcagccacgg tctttccagg taaacattgc ccatagaacc ctcctttcaa
     1861 aaatgcggta agaaacggtt tctgatgcga cgatgcttcg cttaaaacag tgtagccaaa
     1921 aacagatttg ccctgtacgt cggaaaaggt ggaacgttac gcgcggccgc ttattgacgg
     1981 gtcggcccgt ctttgcagct gcatggagaa tgatcagaaa acggtttttt gaagtcccgt
     2041 tttttaacag aggatttaca actttttctt tcggcttccg gaatcgcctg caaaaatccg
     2101 ggacagcgcc cgttttttgc gctttttcca gcttcccttg tggtaggcgt agctggccag
     2161 caggggcaga gcggtggtgg cttccgcgta caccatctgt tcgcaggagg aatccacctt
     2221 gccccaggaa ttggcttcct tgagcgttga gctggagcac gcgccgtcgc gcacgtccgc
     2281 cacggtgatc tgaacggcgt atttgtgcat ggtcacgtca tgcccgagaa tctcggcgca
     2341 gaccacggta tcctgggcga agtttttcgg cacgccgccg ccgatcatca aaagaccggt
     2401 ggtgcctgcc ttgattttta tttcggtcag ctcccggaaa tctttgacgg aatcgatgga
     2461 caggtgattg tccgggtgtt ccacctgatg cagcacgagg ccgaaaccgg cggagctgtc
     2521 cgaaaacgcg gggcagaaga tcggcacatc gttttcatag gccgcctgca ccagtgaatt
     2581 tttctttttg gcgttcttcg tcagatatct gcccatttcg gcgatgaatt cccgcgacga
     2641 ataggggcgg ggttccagcg aatcggcgat ttttttgatg gtctggtcgc agtgctgcaa
     2701 atcgtcttcg ctgatgaacg tgtcataaat gcggtcgatg tacaggctgc gcagcagctt
     2761 gtcgtccacg aacggcgttc cctgatagtg acggaagccc agggcttcaa aaaaatccat
     2821 gtccacaatc gacgcgccgg tcgccacgat cacgtccacc atgttgtttt tcaccagatc
     2881 gacatacacc tgcatgcagc cggccgcgct ggtggagccg gccagcgtca gaatgaccgc
     2941 gcagtttttc tcccggagca tccggtcgta aagttcggcc gccgaggcca ggtcgcgggc
     3001 ggaaaaggac attttcttca tggcccggat gatgtccgcc gcgttgattt tcttgatgtc
     3061 gatgtgctcg acgggcttct tcagcaaatc cttcttcttc atgaattctt tcattctccc
     3121 gtgttttctt cccgaattga atatgctatt aaatcacggc tccttaaaag tcaatcgatt
     3181 acagccgttg attttttccg ctcaaaaagg aaattcatca tcggccggct tcttttgccc
     3241 gaccgtcgtt tcggggctga tatatttctc cgccttttta tgaaccggat ttgcgtgaac
     3301 gacaatggag cggggcgcgg ttgggcaggc cagttgacac gcgccgcagc cgatgcagta
     3361 ttgccggtcg atttccggat agagaatatt tttcttgtcc gcgaaacgaa tcgcgtgcgt
     3421 ggggcagact tcgccgcagg cgccgcaatt attatgatcg acatagacaa cgcaaatatt
     3481 ttccaaaagc tcggcttcgc cgatctgcgt gagctttttc tcctccagag aaagcgacgc
     3541 gagcgcgccg gtcgggcaga cccggctgca gaggctgcat tcataatcac aaaagctttt
     3601 ttcataattc attttcggct gcatcaaacc ggatgcgccg aaatccataa aagccggcgt
     3661 cagaacctgc gtcgggcaga cgctgacgca cagagaacag gcggtgcacg cccgggtgaa
     3721 atgtgcaata tcgcgtgatc cgggcggcgt aaccggcggc tcggcctggg cccggcggat
     3781 atcgatccag gcgcgcagtt tcgtattcaa cgccaaaaga gcggcggcgg ccgcgatgcc
     3841 gcccatgaca aaaccccgcc gcgaaggcga ccaggcgccg gcatggggct gacgttgtct
     3901 tcgccggtag cggatggcgg attgggaaca ggcgtcaagg cagttcaagc agccgatgca
     3961 gcggctcatg tcgacaaagg cgttgtgcga atcaatgcac cccgccttgc acacattttc
     4021 acaccggacg cattcggagc aggctttcgg gttcaccgtc aattgaaaaa cggagaagcg
     4081 cgccaccagt cctaaaaacg ttcctaccgg acagaccgta ttgcaataga gccgcccgcg
     4141 cgttgcggca agatacaaaa tcagcagaaa aaaagcggcg gtcgccgcca aaaccgacag
     4201 gggaacgaag gctgtctgtt tcggatacca gtaaacgtca aaaggcttta acgccgcaat
     4261 gcccaggttg tatgccccga cgacgagcgg ctgaagaaaa tgcgaaaaaa atcttcccgc
     4321 aagcgaatag ggatccagca gactgaccag cgacagggaa ccgatgaccg ccgccgcggc
     4381 cgttgacgcc agcagcgcat agcgcaggcc gttgtgaggt ccggcaaaag cgtgcccggg
     4441 cagaaggccc agccttcggg agcccgcgat gatcaaatcc tgaagcgttc ccagcggaca
     4501 caggaaggaa caatacaccc tgccgaaaat cagcgtcatc agcacgatga agatgaagcc
     4561 ggccataaac agccccgacg gcgccgtcgc ggtttgcacc agagaaggga caaactgaaa
     4621 aggcgcaatg acgtcggaca ggaagacgga ccatttttcc gggccgagaa acagcaggac
     4681 aaacaagaca aagatgatgc cggagatgac tttgcgtatt ttgacgaggg tcaagaagcc
     4741 ggtcctctac atggtgtacg tgacaatttt gagctctttc aaattcatat tgccggtttt
     4801 ttgcgcatgg gcgattttga tgtagccgat gtcttccgga tccttcccga aaatccgggc
     4861 ggcggcggcg tccaccgcca cgatatcctt cgagatcagc agggtctttt tcatctgaac
     4921 atcggcggcc cgcgggttgc ccgggcccgc gggcccgcgg ctcatcagca cgcgataggc
     4981 atccaccacg ttgagatcgg gcctgcggta caggcagaaa tccgcaatgc actggtccag
     5041 gccggtcaga tgatattcca tgcggttttt gacgatgccc atcaggttct tcatggcaat
     5101 ggacagatgc gcggaactgt gatgcttgag caccggcacg ttgatgaaca cgtcgcaatc
     5161 caggatcagg ctgtgaacat ccgccgtctt gagcgttttg cctccgggga ttttgacggt
     5221 gcggtagctg aaatcatcca ccggcacaat gatgccgcgg gcggatttcg ccgcctcttc
     5281 aatgccgctg agcttgtagc agttgcgggc attgccggag gtgggcgtca ccacgttgtc
     5341 aaagacataa acgcgcctgg cgcccgccgc ccagcaggat tcaatgatcg ttttaatcag
     5401 aagcggattg gtggtggcac cgacttccgg cacgcgcgga aaaccgatat tgggcttgac
     5461 gacaaccgtc tgcccttttt tgacaaactg attcatgccg cccgcgagtt caatggcttt
     5521 tttgaacagg gcatcgggtt cgccgttctt gacggcgacc agatcgggaa tgacggccgg
     5581 tttttctttc gccgacagaa atgccggccg gcccagaata accgccccgc ccgcgatcag
     5641 accgagcgac agcccttttt gaaggatttc ccgccggttg aatttagttg cgtctgccat
     5701 gccggcctcc gtttgattgg acgatgctga tacgaacaaa ggcgatgcgc cggtatttgc
     5761 ggaaaaatat agggaaaaat ccgggccgtg tcaattgaaa acggtggata attttttctg
     5821 ttcaaagacc cgtgaatagt atagaatcgc taaaactgaa aagggatgaa ccgatcatgc
     5881 aaaaccccac gataagcgca accggtgttg catcttttcg cagagccgcc acggctttcg
     5941 gtctgctggt gtttttgccc tgcttgttga gcctcatcaa tgtgacgttt gcgcaaagct
     6001 ggaagattca tttctttccg gcggcggttt tcctggcggc cgtcggcttc ggtccggcag
     6061 gcggtctgat cgccggcatc acggggtctc tttattcggc cgtcgcgctt ggcaacccct
     6121 ggctgatcgc gggcaacgct ctcctgggat ttttaaccgg ttttttcttt cgcaggactt
     6181 caaaaattat tctttccgtg ctgctggcgt tcgccgttca gcttccctgg ctgattctgt
     6241 ccgactatta ttttgccggt ctttcacccc tgtttatcgc caaattggtc atggtcttgt
     6301 ttttgggcaa tctgttgtgg gctttgctga tttcgctcgg catgaagccc attaaaaaat
     6361 atctatgctg actcctgatt ttacccgcta ttcactggcg cttctggaag gcgacagact
     6421 gattcattct tccaagggcg gcggtctgcg ccccttatgg gacgctctgg aaaaatattc
     6481 agaaaaatcc ggcctgatcc tgcatgacaa ggtcatgggg cttgccgccg ccagactgat
     6541 tcatcgttcc ggcatcattg ccgaagtgca tacgatggtc gtgtcgctgc ccgccgtgca
     6601 atttctcaaa gaccacggca ttcgcctgac ggcattccat tcggcggcca atatactgac
     6661 ccgcgacaaa agcgccgtct gcccgggtga agcgattgcg ctgggcacag gcgaaccgga
     6721 tgccttttat caaaaaatca gggaaatgat ggagattcct gtcattgcct gaatgccgga
     6781 gacagtcccg agctgcttct ttgggaaaag tcaatcgatt gcggctatgg attgatttct
     6841 tttcgctttc cgggccgggg ccttttgttc ggacgctggg gtttgggcct ctggacggtt
     6901 gggcctggcg cctcagcatc atccgccgcg ggctgcaccg gcggcgcgtg cagggactgc
     6961 tgatagtaat cgtcgagcag catggtaatg atggccagct cttcctcgga ttgcgccaga
     7021 ttgcgcgcca gcggcttaaa acgctgcatg cgctcgatct ggatattgtc gcggccgcgc
     7081 aggcgcgcct ccagaagcgc ggtgatcctc tcggagacaa ccctttccag ctcctcgtcg
     7141 ccggggagtg aacgctcttc catgttaatc gtgtaaaaac gggcgatgct ctgaatcttg
     7201 aatctctcca tttccgcgac cagggaaatg accacgccgc tcgatcccat ccgcccggtg
     7261 cgtccggccc ggtgaatgta ggcttccgga tcttccggcg gctcatactg aaaaacgtgg
     7321 gacagatcgg gaatgtcgat gccgcgcgcc gccacgtcgg tcgcgaccag aaagcgcagc
     7381 gccccgcgcc gcacgcgagc catcaccttt tcccgcgcgg tctgggccag gtcggaggag
     7441 agctcatcgg cgtcgtagcc gaagcgcttc agcacgacgg acaggtagtg caccgtatcc
     7501 ttggtattgc agaaaatgat cgccgaggcg ggattttcaa tctcgatgat cctcaccagg
     7561 gagcgctcct tgcgcatgcc cggcaccacg taatagatgt gctcgctttc ggcgatatga
     7621 acctcgttgc cgcttaagct cagcagttcg ggttcgcgca aaaactggcc ggccaggcga
     7681 atcacctgcg gcggaaaagt ggcggaaaac atgctgcttg cgattttgcc cgtcggaata
     7741 tatttgcgga ttttgaccat gtccggataa aatcccatcg acagcattcg gtccgcttcg
     7801 tcaaaaatca gcattctcag atggtccagc gtcagggtgt tcttgagcag atgatcgaga
     7861 atgcgtcccg gcgtcccgac cacgagttgg gcgcctgacc ggaatgcttc gagctggggg
     7921 ccgtagccga caccgccata aaccaccgcc gtcttcaacc cggtcccctc cgccagcacc
     7981 tgcgaatccc gatagacctg cagggccagt tcgcgggtgg gcaccagaac cagcgcctgg
     8041 gcgacatttt tcgcaagatt gatcttctgg atgatcggca gcaggtaggc ccctgtcttg
     8101 ccgctgccgg ttcgcgactg aatcatcaca tcccggccgg ccagcaggta ggggatggtc
     8161 ctggcctgca ccggcaccag cgacggccag cccgcgcgcg cgcaggccgt tttcaactcc
     8221 ggattcaggt cctccaggca aatttcgcca agaccggcaa cttcagactt ttccatccct
     8281 tcttcgggat tatgctcttg catgatttca tgatcctctc gtcaaaataa tttataaata
     8341 tcacttattt cagagcggct caggaatcaa gaaaaaactt ggaggatcag gaggcttatt
     8401 ccgcgaaggc gggggtctca gaaaatccct tgacaggcgc cgatgtttca tatagcgtag
     8461 cggcgaattc gtcagaggct ctgcgagccc tgcctgttgg agcgattcaa gtat
//
LOCUS       DAOSIM010000067         8459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_7828_length_8459_cov_7.232865, whole genome shotgun sequence.
ACCESSION   DAOSIM010000067 DAOSIM010000000
VERSION     DAOSIM010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8459
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7828_length_8459_cov_7.232865"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1652)
                     /locus_tag="PLR47_04435"
     CDS             complement(<1..1652)
                     /locus_tag="PLR47_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOG12221.1"
                     /translation="MTKNQKIKSLLQKQLVILDGATGTELQKRGMPAGVCPEIWCLEN
                     PAVIQDIHASYAKAGARVVYTCTFGANRFKLKQFGVKKDAASVNRELALQTRQACGRK
                     ALVAGDIGPTGLFIEPFGPLAFEKAVDAFNEQARGLIDGGCDLIVIETMIDIQEARAA
                     LLAVKELGDIFTIASMTFESDGHTLGGTPPEAALITLQSLGADAVGCNCSTGPEQMAE
                     YIAAMKPLATVPLLAKPNAGLPRLEGLKTVFDMDPASFAAGARKLAAAGANLLGGCCG
                     TTPAHIAALAKAVGKKKPASPRRKSIAALSSARSLLSLQDDQPLLVVGERINPTGKKA
                     LQQELLEGKLSIIRRMAQEQEAQGASLLDVNIGLPGIDEAATIKNVIGLLATTTRLPL
                     VIDSSRVETIEAALRLYPGRMLINSISGEKEKLARLLPLAAKYGAMFILLPLTDGSIP
                     KTAEKRREVVRSIYAKVRKAGMTKEDFIVDGLTMAVASDAEAARETLATVAWCNNTLK
                     CKTVLGLSNVSFGMPGRPWLNAAFLAMAQYCGLSMAIANPAGV"
     gene            complement(1751..2794)
                     /locus_tag="PLR47_04440"
     CDS             complement(1751..2794)
                     /locus_tag="PLR47_04440"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12222.1"
                     /translation="MPKTSEALDIKPGKIPVVLLYNIEPDWTAAEKEEAAGHSTKLGL
                     ALLEAGFPTVFVPVADENLEGRLGFFDPAGHIVFNWCESLPGISHSEWMVAGRLEAMG
                     FTFTGAGSETLALAQDKARVKALLDAACIPTPAWRVFHLGEAVRWERFPAIVKPIHEH
                     CSAGISRDSVALDEGELARRVPFILEAYRQPALVEDFIDGREFHVSVWGNEELTVLPA
                     AEMDFAFFKDVKDRLCGHEAKFIPGSEAYEKIETRLPAPLDPEEQKAMEIVCRAAYRA
                     VGCRDYARMDIRLQDGVFYVLDVNPNADISHDASMACAAEAAGISYGRMGAAIVGMAA
                     RRHPVFSQWALKP"
     gene            2904..3248
                     /locus_tag="PLR47_04445"
     CDS             2904..3248
                     /locus_tag="PLR47_04445"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOG12223.1"
                     /translation="MKNTKSMECMRCGRCCLADFTAYVTEEDIGRWKSEGRDDILGVL
                     EREGAVWEGDRLMSRESGTPLGGCRFFAFDGKTFYCAIHETRPAVCRLFEPGSSEICP
                     QYSKKGNTRNHI"
     gene            complement(3239..4168)
                     /locus_tag="PLR47_04450"
     CDS             complement(3239..4168)
                     /locus_tag="PLR47_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG12224.1"
                     /translation="MNQLYEKLMIRKTAGGPGSVRIIWLGTAGMFVTDGKTGILIDPY
                     VSRFGMGRVAFRLPLEPNRPLIQKWAGRFGRDNIAAVIASHSHFDHAADAPYFAIEAH
                     APLVGTSSTLNIGRGAGMEENKLVEMTSGRTLCFGNFTIKFIESAHGPAVLGRIPYPG
                     TIDKPLIPPGRAADYRLGGVFALLIQHPAGAILHHGSAGFKPQMYDGTHADVILLGIA
                     GRGNTDQYLENVVLKTRARLVLPIHMDNFFKPLENGMSLLPTVRFGEFCRKTENHRLS
                     FTMNTLPFCNEVGILPLDEIPKTQRMLQSKNQI"
     gene            complement(4165..4503)
                     /locus_tag="PLR47_04455"
     CDS             complement(4165..4503)
                     /locus_tag="PLR47_04455"
                     /inference="COORDINATES: protein motif:HMM:NF017355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOG12225.1"
                     /translation="MDKPMTYKGYAAKIEYSEKDECLIGRVSNIRDIITFHGDSVKEI
                     KQAFEEAVDHYLDGCAEKNEEPEKPFDEGQVMRMSSALHRMIALAARNENKSVGEWIA
                     EARKKPRDKS"
     gene            complement(4627..5292)
                     /locus_tag="PLR47_04460"
     CDS             complement(4627..5292)
                     /locus_tag="PLR47_04460"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOG12226.1"
                     /translation="MRDLIQHEQFELEVLDRLNSGRFLTNLVFGGGTMLRLCHGLDRF
                     SVDLDFWVTKDFDEATYYRKIEKYLLQFYTLADSAHKHFTILFELKSPQFPRALKIEI
                     RKEAKIIKTETCIAYGANSNIQVLIKTVALEEMMKSKIEAFLSRKEIRDAYDIEFLLK
                     KGIALPAENEILGKLLAGLAKLGKKDFSVKLGTLLDASVRQYYIENGFRILEMRLRDK
                     IMP"
     gene            complement(5280..5876)
                     /locus_tag="PLR47_04465"
     CDS             complement(5280..5876)
                     /locus_tag="PLR47_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12227.1"
                     /translation="MLSKSMNRLQELSRKPYFTLGEVAQTFSLQTASARVLCSRYVRQ
                     GLLVRFKNNIYTTVWKWEGLTRQDLFTIANVLQVPSYISLMTALAYYDVTTQAQNNYQ
                     ESVCLKRSVAYNVREAVFSYVKLQNRFYGDFIKKDGVFIATKEKAFLDAAYLFSFGKY
                     KFDVDSLDMKKLELRKLKSLLSVYPNKTKETVKRLCGI"
     gene            6035..6283
                     /locus_tag="PLR47_04470"
     CDS             6035..6283
                     /locus_tag="PLR47_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12228.1"
                     /translation="MTENMVVNIFQHQVPGFNGGPIRIKGGKTRGNQIRVDKNGAVRL
                     VGQKLPGKCVFAGTVGTAMMRALLFFRFGSFKRLFEAN"
     gene            complement(6316..>8459)
                     /locus_tag="PLR47_04475"
     CDS             complement(6316..>8459)
                     /locus_tag="PLR47_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12229.1"
                     /translation="NRMALSCGIRWRNQRNRHPDRPLIAFMGNTVNDKTKEGKGDDEL
                     SDISKIVDYLAKLSESQRAGLKNVFNNEYKGNLKLTRNPQANDEILLSYGAGDYWGIV
                     NVGNGLNFINEYQGENVDKVTSTAIIIANSEKYLFENIDKPHSPINVLIGSRKFAEGW
                     NCFRVSVIGLINLGKTKGNKIIQIFGRGVRLKGLKSDGKRCDSSHIEDYFALQDNYRD
                     NLKRLETLCVFSLQRSYLETFTEAVSSELEITKSFEIAVTPSIINLNGKDACFDTYKR
                     DLKIFKLSKTSVDVKKVLLYPMERKIEYEYVLNGKEQKAVINNVSFNLDYRTNRNEDG
                     RNIRNSLKQANVTCRAFINNQLFKKALIEQADEANLQIYKGGRKLSEIDVDDVLAYIE
                     AIQYKDELPEHDFDFIETVNSKIAEEFVKKVRNKINWHLNSSIYQYEEELKQSSGEIR
                     GDFIEKYTLVKSFKLSKQEGNKTKQKTEREFNQEIADFTKTIATIKNALIIEKIGNHI
                     YEPLLRENKVVLNDDIKLVPDKLNEGEKKFVKDFAAYMKKKPYKFKNFNVYLMRNVES
                     LKSIGIYLNDDSEVFYPDFIMWLIGKDKVYINFIDPKGQMGTKDFKTEQYKEKVTIAD
                     KAKNPTLPNIEKELKRIHKKDFILNSFILLRDSSDLGKVAQPAWKKKNMIDKNIFRLD
                     WHKLDEKENHADLDKLVDGKSYLDWMMVKAI"
BASE COUNT         1872 a         2200 c         2066 g         2321 t
ORIGIN      
        1 acgccggccg gattggcaat ggccatcgaa agcccgcagt actgcgccat ggccagaaac
       61 gcggcgttga gccaggggcg ccccggcatg ccgaaagaaa cattggaaag gccgaggacc
      121 gttttgcatt tcagcgtgtt gttgcaccag gctaccgtgg ccagcgtttc ccgcgccgct
      181 tcggcatcgg aggcaaccgc catcgtcagc ccgtccacga taaaatcctc tttggtcatt
      241 ccggctttcc ggacttttgc ataaatcgac cggacgacct cccggcgttt ttcagccgtt
      301 ttgggaatgc tgccgtcggt gaggggcagc aaaatgaaca tcgcgccgta tttcgcggca
      361 agcggcagaa gcctggcgag cttttccttt tcgccggaaa tggaattgat cagcattcgg
      421 cccggataga ggcgcagcgc cgcttcgatg gtttccactc tggacgaatc gatgaccagc
      481 ggcagccggg tggttgtggc gagaagcccg atgacgtttt tgatcgttgc ggcttcgtca
      541 atgcccggca agccgatgtt gacgtccaga agggatgctc cctgcgcttc ctgctcctgc
      601 gccattcggc gaatgatgga cagtttgcct tccaggagct cctgctgcag ggccttcttg
      661 cccgtgggat tgatcctttc gccgaccacc agcaggggct gatcgtcctg cagggataga
      721 aggcttctcg cggagctgag cgccgcgatc gactttctgc ggggacttgc cggttttttc
      781 ttcccgaccg ccttggccag cgccgcgatg tgagcgggcg tcgtgccgca gcagcctccc
      841 agcaggttgg cgcccgccgc ggccagcttc ctcgcgccgg cggcaaagct tgcgggatcc
      901 atgtcgaaga cggtcttcag cccttcgagc ctcggcaggc cggcattggg cttggccagg
      961 agcggcaccg tggcgagagg cttcatggcg gcgatatatt ccgccatctg ttcgggaccc
     1021 gtggagcagt tgcagcccac ggcatccgcg cccaggctct gcagggtaat gagcgccgct
     1081 tccggcggcg ttccgcccag cgtgtgcccg tcgctttcaa aggtcatgga agcgatggtg
     1141 aagatgtcgc ccagctcttt taccgccagc agggccgcac gcgcctcctg aatgtcgatc
     1201 atggtttcga tcacgatcag atcgcagccc ccgtcgatca ggccgcgcgc ctgttcgttg
     1261 aacgcgtcca cggccttctc aaaggccagc gggccgaaag gttcgatgaa caggccggtg
     1321 ggcccgatgt cgccggcgac cagcgctttc cggccgcagg cctgccgggt ttggagcgcc
     1381 agttcccggt tgaccgaagc ggcgtctttt ttcacgccga actgcttgag cttgaagcgg
     1441 tttgcgccga acgtgcaggt gtaaaccacc cgggcgccgg cttttgcgta ggaggcgtga
     1501 atgtcctgaa tcaccgccgg attttccagg caccagattt ccggacagac cccggccggc
     1561 attcctcttt tctgcagctc ggtgcccgtc gctccgtcca gaatgacaag ctgtttctga
     1621 aggagacttt tgattttttg attttttgtc acgtcaactc ctgcgggctc tgtttttcgc
     1681 tgagccctta tatcatcttt gcccttccag ggggaatttt ttccatgccg gcattcattt
     1741 ctcttgaaca tcaaggcttt aaagcccatt ggctgaaaac cggatggcgg cgggcggcca
     1801 tgcccacgat ggccgcgccc attcggccat agctgatgcc ggccgcctcc gcggcgcagg
     1861 ccatgctggc atcgtggctg atgtccgcgt tggggttgac gtccaggacg taaaaaacac
     1921 cgtcttgcag ccgaatatcc atgcgggcgt aatcccggca gccgacggcc ctgtaagccg
     1981 cgcggcacac gatttccatc gccttttgtt cttcggggtc cagcggcgcg ggcagcctcg
     2041 tttcgatttt ttcgtacgcc tcggagcccg gaatgaactt ggcctcgtgg ccgcagaggc
     2101 ggtccttgac gtctttaaaa aatgcaaaat ccatttccgc ggcgggcaat acggtgagtt
     2161 cttcatttcc ccagaccgac acgtgaaatt cgcgcccatc gatgaaatcc tccaccagag
     2221 cgggctgccg gtaggcctcc agtataaaag gaacgcgccg ggccagctcg ccctcgtcca
     2281 gcgcaacgga atcgcggctg atcccggcgg agcagtgctc atgaatgggc ttgacgatcg
     2341 cgggaaacct ttcccaccgg acggcttcgc caaggtgaaa gacgcgccag gcgggcgtgg
     2401 ggatgcaggc cgcgtccagc agggctttga cccgggcctt gtcctgcgcg agcgcaagcg
     2461 tttccgaacc cgcgccggta aaggtgaagc ccatcgcctc aagacgtccg gccaccatcc
     2521 actcgctgtg ggaaattccg ggcagacttt cgcaccagtt gaaaacgatg tgtccggccg
     2581 gatcaaaaaa gcccagacgg ccctcgagat tctcgtcggc caccggcacg aaaaccgtcg
     2641 ggaaaccggc ctcgagaagc gccaggccga gctttgtgga gtggccggcc gcttcttcct
     2701 tttcggcggc cgtccagtcc ggctcgatat tgtacagcag aaccacggga attttgccgg
     2761 gttttatgtc caacgcctcg gaagttttgg gcatttcttt ttcctttcag ggtagctcat
     2821 taaccatatt ttttttggaa ttccaagaat gaatttacaa acgtccgggc cggtggtatt
     2881 tttcgcgccg gagaagcggc gagatgaaaa acacaaaatc catggagtgc atgcgctgcg
     2941 gccgctgctg cctggccgat ttcacagcct acgtgacgga ggaagacatc gggcggtgga
     3001 agtccgaagg cagagacgac atactgggcg tcctggagag ggagggagcc gtctgggagg
     3061 gcgaccgcct gatgtcgcgg gagtcgggaa ccccgctggg cggctgccgt ttttttgcat
     3121 ttgacggaaa gactttttat tgcgccatcc atgaaaccag acccgcggtg tgcaggcttt
     3181 tcgagccggg ttcgtccgaa atctgcccgc agtattcaaa aaagggaaac acacggaatc
     3241 atatttgatt tttcgattga agcatgcgct gcgttttcgg aatttcatcc agaggcagaa
     3301 tcccgacttc gttgcagaag ggcagggtgt tcattgtgaa ggacaaccgg tgattttccg
     3361 ttttccggca gaactcaccg aatcgcaccg tggggagcaa tgacatgccg ttttccagcg
     3421 gcttgaagaa attgtccata tgaatcggaa gcacgagccg cgcgcgggtt ttcaatacga
     3481 cattttccag gtattgatcg gtgttccctc ttccggcgat gcccagaagg atgacgtcgg
     3541 catgagtccc gtcatacatt tgcggtttga agcctgcgct tccatggtgc agaatggctc
     3601 cggcggggtg ttggatcagc agggcaaaga cgccgcccaa acggtagtcg gcggcccggc
     3661 cgggcgggat caggggcttg tcgatggttc cggggtaggg gatgcggccc aggacggcgg
     3721 ggccatgagc gctttcaatg aattttatcg taaagtttcc gaaacacagc gtccggccgc
     3781 ttgtcatttc caccaatttg ttttcttcca tgccggcgcc tctgccgatg ttcagcgtgc
     3841 ttgacgtgcc gacgagcggc gcatgggctt cgattgcgaa atacggcgcg tcggcggcat
     3901 ggtcgaaatg gctgtggctt gcgatgaccg cggcaatgtt gtccctgccg aacctcccgg
     3961 cccatttttg aatgaggggc cggttcggtt ccagcggcag acgaaacgca accctgccca
     4021 tgccgaaccg gcttacgtaa ggatcaatca ggatgcctgt cttcccgtcg gttacaaaca
     4081 tgccggcagt gcccagccag atgatgcgga cgctgcccgg ccctccggcg gtttttctga
     4141 tcatcagttt ctcatagagc tggttcatga tttgtcccgg ggctttttgc gggcttcggc
     4201 aatccactcg ccgacgcttt tattttcatt cctggcggcc aaagcgatca tgcgatgaag
     4261 cgcggaactc atccgcatca cctggccttc atcaaaaggc ttttccggct cttcgttttt
     4321 ttcggcgcag ccgtcaagat aatggtcaac ggcttcctcg aaagcttgtt tgatttcctt
     4381 caccgaatcg ccatgaaagg ttatgatgtc acgaatattg gaaacgcggc cgatcaggca
     4441 ttcatctttt tcgctgtatt caatctttgc ggcatagcct ttatacgtca tgggcttgtc
     4501 catgggtttg cctccagttg ctccggtacg tctcacatta ataaatggca cctgtccata
     4561 aagatgtttt tcctccctcc cgtcatgatg atcagtgttg tcctcagacc tcgaatattc
     4621 gatggcttat ggcataattt tatcccgcag tctcatttcc agaatgcgga atccattttc
     4681 aatgtaatat tgtcgcaccg atgcatcaag caacgttcca agctttacgg aaaagtcttt
     4741 cttgccgagt tttgccaatc cggcaagcaa tttgcccagg atttcatttt cagcgggaag
     4801 ggcgatgcct ttttttaaca aaaactcgat gtcgtatgca tcgcggattt ccttgcggct
     4861 gagaaaagcc tcaatctttg acttcatcat ttcttccagc gcaacggttt ttatcagaac
     4921 ctgaatattg gagttggcgc cgtaagcgat gcaggtttcc gttttgatga tctttgcctc
     4981 tttgcggatt tctattttca gcgctcttgg aaattgcggt gatttcaatt cgaacaaaat
     5041 cgtaaaatgc ttgtgggcgg aatcagcaag agtataaaat tgcagcaaat atttttcaat
     5101 cttccggtaa tacgttgcct cgtcaaaatc ctttgtcacc cagaaatcca aatccaccga
     5161 aaaacgatcc agtccatggc ataaacgcag catggtgccg cccccgaaaa ccaagttggt
     5221 cagaaatcgt ccgctgttca gccggtccag gacttccagt tcaaactgct catgctgaat
     5281 taaatcccgc atagtctttt caccgtttct tttgttttgt tcggataaac gctcaacaga
     5341 ctcttcagct ttctcaattc cagcttcttc atgtccagcg agtcaacgtc aaacttatat
     5401 ttgccgaacg agaaaagata cgccgcatcc agaaaagctt tttctttggt ggcgataaat
     5461 acgccgtcct ttttaatgaa atcgccatag aagcggtttt gtaatttgac ataactgaaa
     5521 acggcctccc ggacattgta agcaacagaa cgtttcaggc aaacgctttc ctgataattg
     5581 ttctgcgcct gcgtggtgac atcataatac gccagggccg tcatcaggga aatataagag
     5641 gggacctgca atacattggc gattgtaaac aaatcctggc gtgtcagacc ttcccatttc
     5701 cagacggttg tataaatatt atttttaaat ctgaccagca aaccctgccg gacataacgg
     5761 ctgcacagta cacgagcgga ggccgtctgc aggctgaaag tctgcgcaac ttcgccaagc
     5821 gtaaaatagg gcttccggct taattcctgc aatctgttca tgcttttact aagcatatat
     5881 aattaccttt gatgttaatt atatatactt tgtaatattt ggcaagcgaa ataaagcgct
     5941 taacagggct gagagaaaac gcaatagcaa cggttagtta aatatcatgg aatggtgtgt
     6001 tttgtgcccg gaatcaataa aacgcaaaac tttcatgact gagaacatgg tagtaaatat
     6061 ctttcaacat caggtgcccg gttttaatgg cggcccgatc cggatcaaag ggggcaaaac
     6121 ccgcggcaat caaatccgcg ttgacaaaaa tggggcagtc cgcctcgttg ggcaaaaact
     6181 ccccggcaaa tgtgtttttg ccggcaccgt cggtacggcg atgatgaggg ctcttctttt
     6241 ttttcgcttc ggttcattta agcgtctctt tgaagcaaat tagttttcga actaattgtg
     6301 cctgtcccgt tatttctata tcgctttcac catcatccaa tccaaatagc ttttgccatc
     6361 aaccaattta tctagatcag cgtggttttc tttctcatcc aatttatgcc agtcaagcct
     6421 gaatatattt ttatcaatca tatttttttt cttccacgca ggctgtgcga ccttacctaa
     6481 atctgaactg tcgcgtaaca atataaaaga gtttaatata aaatcctttt tgtgaatccg
     6541 cttcagctct ttttcaatat ttggcaatgt aggattctta gctttgtctg caatcgtaac
     6601 tttctcttta tactgttctg tcttgaagtc ctttgtgccc atttgacctt tcggatcgat
     6661 aaagttaatg taaactttat ctttgccaat tagccacatg ataaaatccg gataaaacac
     6721 ttctgaatcg tcgttcaagt aaatgccaat tgatttcagc gactctacat tccgcatgag
     6781 gtaaacattg aaatttttaa atttatacgg cttttttttc atgtatgccg caaagtcttt
     6841 tacaaatttc ttttctcctt cattgagctt atccggcacg agctttatat catcgtttaa
     6901 aacaacttta ttctctcgca ataatggctc atagatatga ttgccaattt tttcaatgat
     6961 cagcgcattt tttattgtgg caatcgtttt tgtaaaatcc gctatttctt gattaaattc
     7021 tctctcagtc ttctgttttg tcttatttcc ttcctgcttg gatagtttaa aagatttaac
     7081 caatgtatat ttttcaataa aatctcctct gatttcaccg ctactttgtt taagttcttc
     7141 ttcatattga taaatgctgc tgtttaagtg ccagtttatt ttattgcgca ctttcttgac
     7201 aaattcttca gcgattttgc tgttgacagt ttcaatgaaa tcaaaatcgt gttcaggtaa
     7261 ttcatctttg tattgaatcg cctcaatgta agccagaaca tcatcgacat ctatttctga
     7321 aagttttctt ccgcctttat atatttggag attggcttca tcggcttgtt cgatcagcgc
     7381 ctttttgaat agttgattgt taatgaacgc ccggcaggta acattcgctt gcttcaatga
     7441 atttctgata ttgcggccat cttcatttcg atttgtcctg taatccagat taaaactgac
     7501 attattaatg actgcttttt gctccttacc attcagtacg tactcatatt cgatttttct
     7561 ctccattgga taaagcagca cctttttgac atcaactgat gttttactga gcttgaatat
     7621 cttcaaatcg cgtttgtacg tatcaaaaca agcatctttg ccatttagat tgatgatgct
     7681 tggtgtcacg gcgatttcaa atgatttcgt tatttccaat tcactgctta cggcttccgt
     7741 aaaagtctcc agataactgc gttgcaaact aaaaacgcac agagtttcca atcgtttaag
     7801 attgtctctg taattatcct gtagggcaaa ataatcttca atatgactgc tgtcacatcg
     7861 tttcccatca cttttcaagc ccttcaagcg aacaccgcgc ccgaatatct ggataatttt
     7921 attgcctttg gttttcccca gattaatcaa accaatgaca gaaacacgaa agcagttcca
     7981 tccctcggca aacttccggc tgccgattaa gacgttaatt ggcgagtgcg gcttatcaat
     8041 attttcaaaa aggtattttt cgctattggc aataatgatg gctgtgctgg ttactttatc
     8101 aacgttctcg ccttgatatt cattgataaa gttcaagcca ttgcccacat tgacgatacc
     8161 ccagtaatcg cctgcgccgt aagaaagcaa tatctcatca ttggcctgcg gatttctggt
     8221 cagtttcagg ttgcccttgt attcattatt aaaaacattc tttaatcctg ctctttgtga
     8281 ttcagaaagc tttgccagat aatctacaat cttggaaata tccgaaagtt catcatcgcc
     8341 ttttccttct ttggttttat cgttgactgt attgcccatg aaagcaatca acggcctatc
     8401 ggggtgccta ttccgctgat ttcgccacct gattccgcag gaaagtgcca tgcgattcc
//
LOCUS       DAOSIM010000068         8421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_7882_length_8421_cov_7.987449, whole genome shotgun sequence.
ACCESSION   DAOSIM010000068 DAOSIM010000000
VERSION     DAOSIM010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8421
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7882_length_8421_cov_7.987449"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="PLR47_04480"
     CDS             <1..1319
                     /locus_tag="PLR47_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG12230.1"
                     /translation="TPFMGSSVNVALPAIGRAFSMDAISLSWVASSFLLASAIMLLPM
                     GRLADIYGRKIFFLSGSLIFALSSLLAVLPETESLFIALRVAQGFGAAMIFSTGTALL
                     VSAYPAGERGKMLGINIAAVYTGLTAGPLIGGLLTQHLGWQAIFIANAALGAGAAGLA
                     LRMEDEKTPAAAGEPFDLSGSFLYAAALFALMYGFSRLPSSRGAVLIAAGGIFFIIFI
                     RRQLQKPSPLINIRLFLRNRVFAFSNLAALIHYSATFAVTFLMSLYLQHVKGLTPSQT
                     GFLLVIEPLLQAVFSPLAGRLSDRHEPRIISSIGMSITVVGLAGLIFVARDTPLWTIL
                     FCLALLGTGFALFSSPNVNAVMSSVDGKFYGIASAMQATMRMVGQMLSMGIAMLVFAW
                     IIGSRGIDYENGELLITSAKIIFAILAVICVGGILASLARGRVHKA"
     gene            1339..2151
                     /locus_tag="PLR47_04485"
     CDS             1339..2151
                     /locus_tag="PLR47_04485"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOG12231.1"
                     /translation="MIVNGDAVYFCERGLKMRTQWISFAIGLAAGCFGGLIGIGGGII
                     MVPLLVEILHVDQCRAHGTSLVVLIFTGISGAVSYGLHGQVNWKAAVLLAVPAMLLAA
                     AGARLADRLPDWKLKKFFGGFLIFCSALLLLKPILADWIGVLPDAVSNVALVVTGGAT
                     GFLSGLMGIGGGTIMIPAMILLGGFTQHVAQGTALLVMVPSGFVGAFAHWKLGNVAGK
                     LLFSMVPGIVLGAFAGAGIAQFIPEEPLRWMFVLVAVYMGWRYVKVVSPNTC"
     gene            complement(2214..3482)
                     /locus_tag="PLR47_04490"
     CDS             complement(2214..3482)
                     /locus_tag="PLR47_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG12232.1"
                     /translation="MNAKRNPWLWVPSIYIAEGIPYMIVMTVSVVMYKNLGLSNTDIA
                     LYTGWLYLPWVIKPLWSPFVDLFRTKRFWIVAMQLAIGGSFACVALTLPASDFVRYTL
                     AMFWIMAFSSATHDIAADGFYMLGLTTPEQAAFVGVRTVFYRVATIASKGGLVILAGT
                     LQKCGYPAACAWSITFVVVGAFFLALCLYHLLVLPYPEADHSAPLDKGRGVVAEYFRI
                     ITLFFRRKDIVVIICFFLFFRFAEAQLVKMVAPFLLDSRAEGGLGLSTAQVGWIYGTV
                     GVVALMLGGLLGGYVIYRNGLKFWLWPMVIIMHVPDVIFVYLSHAQPDNIWLIGSAVA
                     AEQFGYGFGFTAYTMYMIMVSQGEYKTVFYAIGTGIMALGMMLPAMSSGWIQEKLGYI
                     HFYYWILLTTIPGFIVTALVKIDPEYGKKA"
     gene            complement(3674..4996)
                     /locus_tag="PLR47_04495"
     CDS             complement(3674..4996)
                     /locus_tag="PLR47_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOG12233.1"
                     /translation="MIDHEPNIQAALLQHTSEARLILEGDFILPDGRVIKGGYMVRAD
                     GGEVFLTDTEGRGGIRGKDILLSPAAPAESFFTVSGIKIGIDFHWQRAQKQSFRGALL
                     LSGQGQSAFHLINIVPLEEYLASVISSEMAASAPLEFLKAQAVTARSWLTAMLAKKKS
                     IRARHPKTKGEIIVWQDVNDHQGFDVCADDHCQRYQGITRIVSENVNRAIDATRGIFL
                     TSAGEICDARYYKCCGGRTDVFATAWEDASFPYLTSITDGKKQRAPIHTEKDAAEWLR
                     AKPDAYCNTNDPEILRKILPSFDLETLNFYRWRVEYQPEELEEIIRSKSGIDFGRLKN
                     IEALQRGPSGRICRLKIEGSLGAAVVGRELEIRRWLSPTHLLSSAFVVSAKRDPDGNA
                     LGFLLEGGGWGHGVGLCQIGAAVMASQGRSAEEILSHYFTGAMLKKLY"
     gene            5230..6150
                     /locus_tag="PLR47_04500"
     CDS             5230..6150
                     /locus_tag="PLR47_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG12234.1"
                     /translation="MAHIWPIGGGKGGSGKSFITSSLGRLSAGLGRKTLVIDLDLGAA
                     NLHTLVGVPYPSKSLSDFLQKKTERLDDTVVETPFENLFLISGAQDHFAIANLPHEQK
                     VKTMRAIMKLDYDCILLDLGAGTSFNTLDFFLISQNGIFITTPEPTSIENVYRLIRSV
                     YFRRIRYGFSVSQFKALEEEAIEQFGEGASGRPEFMMNVIARDYPERFRELQKDFDAF
                     RFRLIINQLHRRDNPALGAHVCRIIEKHMNLHVEFMGNVSYDDHVHDAICQQVSYLDR
                     YPYTKTAGELRAAGRQIFQPAGRQLMLQYV"
     gene            6191..6871
                     /locus_tag="PLR47_04505"
     CDS             6191..6871
                     /locus_tag="PLR47_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG12235.1"
                     /translation="MRKSFAELNYYEMLDVSPTAAAFEIRQAYGTALQIYRTGSAASH
                     SFFTEAERREILSLVEKAYRTLADELTRKAYDDELASRGEIDRPAQAGPDAKKPVGIF
                     NISRVRDRQPVPANQESLKSKIRESERISELTARSSLSGAGLKEIREELGVAIAQIAQ
                     ATKVRQDHLVNIEEDRVERLPAAIFLKGFVKSYLKYLCLEPVEELSARYMETIARLLR
                     REGAQEQE"
     gene            6885..7550
                     /locus_tag="PLR47_04510"
     CDS             6885..7550
                     /locus_tag="PLR47_04510"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG12236.1"
                     /translation="MESDTIQTPEGDLVIRFVGHSSLIFFWAGKNIHADPVSLEADYA
                     RLPKADVILITHGHGDHMDVNAVNIIKIPATKIVGNDEVGSQIPGTLVMKNGEERVVE
                     GLKIEAVPAYNLKHMRAPGKPFHPKGVGNGYVVTLGGLRVYIAGDTENIPEMRRLQNI
                     DIAFLPMNLPYTMTPEMAADAAQMFHPKVLYPYHQGETDTAKLLALMKDHKDIDVRIR
                     TMK"
     gene            7622..>8421
                     /gene="dapF"
                     /locus_tag="PLR47_04515"
     CDS             7622..>8421
                     /gene="dapF"
                     /locus_tag="PLR47_04515"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOG12237.1"
                     /translation="MNGNGVAFWKASGSGNDFIIIDNRDLSLNVGELPAFARSVCRRK
                     VSVGADGLLLVEPSAVADFRWRFFNSDGSEAAMCGNAARCVARFAFENGIAGQTMSFE
                     TQAGIIRAEVARDVAKVRLTDPSSLKQDIRIHLSGGECGLDCIDTGVPHAVCFVASAE
                     ACDVTATGREIRRHAVFQPEGANANFALVINRHKMRVRTYERGVEDETLACGTGVVAS
                     VLAAAGRRFVDSPVDVTVQSGETLRVYFSRQDGAFSEIYLEGKVKIVY"
BASE COUNT         1741 a         2352 c         2479 g         1849 t
ORIGIN      
        1 cgacgccctt catggggtcg tccgtgaatg tcgcccttcc cgccatcggc cgggcttttt
       61 ccatggatgc aatttccctg agctgggtgg cgtcttcgtt tcttctggca tcggccatca
      121 tgctgctgcc gatggggcgg ctcgcggata tttacgggag aaaaatattt ttcctttccg
      181 gatcgctgat ttttgctctc tcctcgcttc tggccgtctt gccggaaacg gagagccttt
      241 ttatcgcgtt gcgcgtcgct cagggattcg gcgccgcgat gatcttcagc accggcacgg
      301 ctctcctggt ttccgcctat cccgcgggcg agcgcggcaa aatgctgggg atcaacattg
      361 ccgccgttta caccggcctc accgccggtc cgttgatcgg cggcctgctg acccagcacc
      421 tgggatggca ggccattttt atcgcaaacg ccgcgctggg agcaggtgcc gcggggctgg
      481 ccttgcggat ggaggacgaa aaaacgcccg ccgcggcggg agaaccattt gacctgagcg
      541 gttccttcct gtacgcggct gcactgtttg ccctgatgta cggcttttcg cggcttccct
      601 cttcccgcgg cgccgttctg attgccgcgg gcggaatctt tttcattatt ttcatccgcc
      661 gtcagctcca aaagccctcg ccgctcatca atatccgcct gtttctgcgc aaccgggttt
      721 ttgccttctc caacctggcc gccctgattc actacagcgc aacctttgcg gtcacgttcc
      781 tgatgagtct ttatctgcag cacgtcaaag ggctcacgcc ttcccagacc ggttttttgc
      841 tggtcatcga acccctgctg caggcggtct tctcgccgct ggcggggcgg ctgtccgatc
      901 gccatgaacc ccgcatcatt tcctcgatcg gaatgtcaat cacggtcgtc ggcctcgccg
      961 gtttgatttt cgttgccagg gatacgccgc tttggacgat tctgttttgt ctggcgcttc
     1021 tgggaaccgg cttcgcgctt ttttcttctc ccaacgtcaa tgcggtcatg agctcggtgg
     1081 acggcaaatt ttacggcatc gcgtccgcga tgcaggcgac catgcggatg gtggggcaaa
     1141 tgctgagcat gggcattgcc atgctggttt tcgcctggat catcggcagc cgcggcattg
     1201 actacgaaaa cggcgagctg ctgatcacga gcgcgaaaat catttttgca attctggcgg
     1261 tgatctgcgt gggcggcatt ctggcttcgc tggcgcgcgg gcgcgtacat aaggcttgat
     1321 tttcaggacg tgatccgtat gatcgtaaac ggcgatgccg tttatttttg tgaacggggg
     1381 ttgaaaatga ggacgcagtg gatcagtttt gccatcggtc ttgccgccgg ctgtttcggc
     1441 ggactgattg gaatcggcgg tggaatcatc atggttcccc tgctggtgga gatactgcat
     1501 gtggatcagt gccgcgcgca cggcacgtcc ctggtggtat tgatttttac gggaatcagc
     1561 ggggccgtct cttacggact gcacggccag gtgaattgga aagcggccgt tctgctggcg
     1621 gttccggcca tgctgctggc cgcggcgggc gcccgtctgg ccgaccggct gcccgactgg
     1681 aagctgaaaa aatttttcgg cgggtttctg attttttgct ccgcgctcct gcttttgaag
     1741 cccattctcg ccgattggat cggcgttctg ccggatgccg tctccaatgt cgccctggtc
     1801 gtgacggggg gcgctacggg atttctttcc ggtctgatgg ggatcggcgg cggaacgatc
     1861 atgattcccg ccatgatcct tctgggaggc tttacacagc acgttgccca gggaaccgct
     1921 ctcctggtca tggtcccttc aggattcgtc ggcgctttcg cgcactggaa gctgggcaat
     1981 gtggcgggca aactgctgtt cagcatggtg ccgggcattg tgctgggcgc ctttgccggc
     2041 gccggaatcg cccagtttat tccggaggag cccttgcgct ggatgtttgt cctggtggcg
     2101 gtttacatgg gatggcgcta tgtaaaagtc gtctcgccga atacgtgctg aagcaccggc
     2161 ttgcggtttt caggccccca tgaaccttat ggctgcggcc cgaaccccgt cgcctacgcc
     2221 ttcttcccgt actccggatc gatctttacc agcgccgtga cgatgaatcc cgggatcgtc
     2281 gtgagcaata tccagtagta gaaatgaata tatccgagtt tttcctgaat ccagccgctg
     2341 gacatggccg gcagcatcat gcccagcgcc atgattcccg tgccgatggc gtaaaagacg
     2401 gttttgtatt caccctgtga aaccatgatc atatacatcg tataggcggt aaatccgaat
     2461 ccgtagccga attgctcggc tgctaccgcg gaaccgatca gccagatatt atccggctgc
     2521 gcgtgcgaca gatacacgaa aattacatcg ggcacatgca tgatgatcac cattggccac
     2581 agccagaatt tcaggccgtt gcggtaaatg acatagccgc ccaaaagccc gcccagcatg
     2641 agcgccacaa cgcccacggt gccgtaaatc catccgacct gggcggtgga aagccccagg
     2701 ccgccttcgg cgcgggaatc cagaagaaac ggcgcgacca tcttcaccag ttgcgcttcg
     2761 gcaaagcgga aaaacaaaaa aaagcagatg atgacgacaa tatctttgcg gcggaaaaac
     2821 agggtgatga tccggaaata ttcagccaca acgccccggc ctttgtccag cggcgcgctg
     2881 tggtcggctt cgggataggg cagaaccagc aaatgataca ggcagagagc cagaaaaaaa
     2941 gcgccgacga ccacgaatgt gatcgaccaa gcgcaagctg ccggataacc gcatttttgc
     3001 agcgtgccgg ccagaatcac caggccgccc ttggacgcaa tggtggcgac gcggtaaaaa
     3061 acagtgcgca cgccgacaaa ggcggcctgt tcgggcgtgg tgagccccag catataaaag
     3121 ccgtcggcgg cgatgtcatg cgtggcggaa gaaaaggcca tgatccagaa catggcgagc
     3181 gtatagcgca caaaatccga tgccggcagc gtgagggcga cgcaggcaaa tgatccgccg
     3241 atggccagct gcatggcgac gatccagaag cgcttggtgc ggaaaagatc gacgaagggg
     3301 ctccacagcg gtttgatcac ccagggcagg taaagccagc cggtgtaaag agcgatgtcg
     3361 gtgttggaaa gccccagatt tttgtacatc acgacgctga cggtcatgac gatcatgtag
     3421 ggaatgcctt cggcgatgta gatggacggt acccagagcc aggggttgcg cttcgcattc
     3481 atgcgcctgc ctttgctggc tttgtcatcg tctcctcggc gtatggcgcc atacgcctgc
     3541 gtcgcctctt tcccgccgcc tcggcatgct tttgactgcg aagcgcgacc atcgtttgca
     3601 tgttgattgg gatcagacat gaagttcctt tagttggctc ttgcacccgt tttccaggtc
     3661 atccttgagg acgtcaatac agttttttca acattgcgcc ggtaaaataa tgcgacagaa
     3721 tctcttcggc gctccgtccc tgagacgcca tcaccgccgc cccgatctga cacaggccca
     3781 cgccgtgtcc ccatccgccg ccctccagaa gaaagccgag cgcgtttccg tccggatccc
     3841 gtttggctga gacgacgaaa gcgctggaga gcaaatgcgt gggcgaaagc cagcggcgga
     3901 tttccagctc cctgccgaca accgccgcgc cgagcgagcc ttcgattttc agccgacaaa
     3961 ttcttccgga cggaccgcgc tgcagcgcct cgatattttt cagcctgccg aagtcgatgc
     4021 cggatttcga cctgataatt tcctccagct cttcgggctg atattcaacc cgccagcgat
     4081 agaaattcaa ggtctccaga tcaaacgagg gcagtatctt ccgcagaatt tccggatcgt
     4141 tggtgttgca gtatgcgtcg ggttttgcgc gcagccattc ggccgcgtct ttttccgtat
     4201 ggatcggcgc gcgctgtttc ttgccgtccg tgatgcttgt cagatagggg aaagaggcat
     4261 cctcccatgc ggttgcaaaa acatccgtcc gtccgccgca gcatttatag tagcgcgcat
     4321 cgcagatttc acccgcgctg gtcagaaata ttccgcgtgt ggcgtcgatg gcccggttga
     4381 cattttccga aacgatgcgg gtgatgccct gatagcgctg acagtggtcg tcggcgcaca
     4441 cgtcgaagcc ctgatgatcg ttcacatcct gccaaacgat gatttcgcct tttgtttttg
     4501 gatggcgtgc ccggatggat ttttttttcg ccagcatcgc cgtcagccag cttcgcgcgg
     4561 tgacggcctg tgctttgaga aactccagag gggcgctggc cgccatttcc gaagaaatca
     4621 ccgaggccag gtattcctcg agcggaacga tattgatgag gtgaaaggcg gattggcctt
     4681 gtcccgaaag caaaagcgct ccccggaatg actgtttctg cgcccgctgc cagtgaaaat
     4741 cgatgccgat ctttatgccg gatacggtaa aaaaagattc agcgggcgcg gcgggagaaa
     4801 gcagaatgtc tttgccgcgg atcccccccc ggccttctgt gtcggtcaga aaaacctcgc
     4861 cgccgtcggc gcggaccata tatccgcctt tgataacgcg cccgtcggga agaatgaaat
     4921 cgccttccag gatcagccgg gcttcggaag tatgctgcag cagggccgcc tgtatgttcg
     4981 gttcgtgatc gatcattccc tcatcccaca gcgcgaaaag tcacaggcct tcttaaaaac
     5041 aatagtggga taagtcaaga aagttgtccc ggaccctgct gtgattttcc gggtgaaggg
     5101 ctgctccatg cggtcccgcc gggtgaaaaa tttcaaactg tttaaaatca ttgacaatat
     5161 caaacttttt tcttgaatcc tgttcgtttt tcgtgtagga aactactaaa atcattgagc
     5221 ggatggttta tggcgcatat ttggccgatt ggcggaggca aaggcggctc cggcaaaagc
     5281 tttatcacca gttcgctggg acggttgtcg gcggggctgg gcagaaaaac gctggtgatc
     5341 gatttggatc tgggagcggc caatctgcac acgctggtcg gggttcccta tccttcgaaa
     5401 agtttgtccg attttcttca gaaaaagacg gaacgcctgg acgacacggt cgtggagacg
     5461 ccttttgaaa acctctttct catcagcggc gcccaggacc atttcgcgat tgccaatctt
     5521 ccccacgaac agaaggtcaa gacaatgcgc gccatcatga agctggatta cgactgcatt
     5581 ctgctggatc tgggcgccgg aacgtcattc aacacactcg atttttttct gatttcccaa
     5641 aacggcattt tcattaccac gccggagccc acctccattg aaaatgtgta ccgcctgatc
     5701 cgatccgttt atttccggcg aatccgttac ggtttcagcg tgtcgcaatt caaggcgctg
     5761 gaggaagagg cgatcgagca gttcggcgag ggcgcctcgg gccggccgga gtttatgatg
     5821 aacgtcatcg cgcgggacta cccggagcgc tttcgggaac ttcagaagga ttttgacgcc
     5881 ttccggttca ggctcatcat caaccagctt cacaggcggg acaatcccgc ccttggagca
     5941 catgtttgca gaattatcga aaaacacatg aacctgcacg tcgaattcat gggcaatgtc
     6001 tcttacgacg atcatgtcca cgatgcgatt tgtcagcagg tgtcttatct ggaccgttac
     6061 ccttacacga agaccgcggg cgagctgcgc gccgcgggcc ggcagatctt tcagccggcg
     6121 ggccggcaat tgatgcttca gtacgtgtag aaagagccgg gcttggcgca caaccgtcgg
     6181 ggaggcctga gtgcggaaaa gttttgccga gttgaactat tatgaaatgc tggacgtgag
     6241 cccgaccgcg gcggcgtttg aaattcgtca ggcgtacggc acagccctgc agatttaccg
     6301 gaccggttcg gcggcgagcc attccttttt caccgaggcg gaaagacggg aaatcctttc
     6361 tctggtggag aaagcttaca ggaccctggc ggatgaactc acccggaaag cctacgacga
     6421 cgaactggca agccggggag aaatcgaccg gccggcgcaa gccgggcccg acgccaaaaa
     6481 gccggtcggc atattcaaca tcagccgcgt ccgcgaccgt cagccggtcc cggcaaacca
     6541 ggaatccctg aaaagcaaaa tccgcgaaag cgaacgaatc agcgagctga cggcccgcag
     6601 ttccctgagc ggagcaggcc tgaaggagat tcgcgaggag ctcggtgtgg cgattgcgca
     6661 gattgcgcag gcgacaaaag tccggcagga ccatctggta aacatcgaag aagatcgcgt
     6721 cgagcgtctg cccgcggcca ttttcctcaa ggggtttgtc aaatcctatc tgaaatatct
     6781 ctgtctggag ccggtggagg aattatccgc ccgctacatg gaaaccatcg cgcgtctgct
     6841 gcgaagggaa ggggcgcaag aacaggagta agaaaggagc ggttatggaa tccgatacca
     6901 ttcaaacgcc cgaaggggat ctggtcatcc ggtttgtggg gcacagctcg ctcatctttt
     6961 tctgggcggg caaaaacatt cacgcggacc ccgtgtcgct ggaagcggat tatgcgagac
     7021 tgccaaaggc tgatgtgata ttgatcactc acgggcacgg ggatcacatg gatgtgaatg
     7081 ccgtgaacat catcaaaatc cccgcgacca aaattgtcgg caacgatgaa gtcggcagtc
     7141 agatccccgg gacactggtg atgaaaaacg gcgaggagcg ggtggtggag ggcctgaaga
     7201 tcgaggcggt gccggcctac aacctgaagc acatgagagc gccgggaaag ccctttcacc
     7261 cgaagggcgt cggaaacggg tatgtcgtta ctctgggcgg tttgcgcgtc tatattgccg
     7321 gcgacacgga aaacattccg gagatgagac ggcttcagaa catcgacatt gcctttctgc
     7381 cgatgaatct gccttatacc atgacgccgg aaatggcggc cgacgccgct caaatgtttc
     7441 atccgaaagt cctgtatccc taccatcagg gcgagacgga tacggcaaag ctgcttgcgc
     7501 tgatgaagga tcacaaggac attgacgttc gcattcggac aatgaagtga caaagcgcgc
     7561 gtcaaccgcc gccggctgaa aagtttcgcg cgaggattta gagacggagg aatggattca
     7621 aatgaacggc aacggcgttg cattctggaa agcaagcggc agcggcaatg attttatcat
     7681 catcgacaac cgcgatttat cgctgaacgt gggagaattg ccggcttttg cccgaagcgt
     7741 gtgccgaaga aaagtgtcgg tcggcgcgga cggtctgctg cttgtcgagc cctctgcggt
     7801 cgccgatttc cggtggcggt ttttcaattc cgacggctcc gaggcggcca tgtgcggcaa
     7861 cgcggcgcgt tgcgtggcgc gctttgcctt tgaaaacggc attgccggac aaaccatgtc
     7921 tttcgaaacg caggccggca tcatccgcgc ggaagtggcc cgcgacgtcg cgaaagtccg
     7981 gctgaccgat ccctcttccc tgaagcagga cattcggatt catctgtcgg gaggcgagtg
     8041 cggactggac tgcatcgaca cgggcgtgcc ccacgccgtc tgctttgtcg cgtccgccga
     8101 ggcgtgcgac gtgacggcca ccggaaggga aatccgccgg cacgcagtgt ttcagcccga
     8161 gggcgcaaac gcgaattttg cgctggtgat caaccggcat aaaatgcggg tccggaccta
     8221 cgaacggggc gtcgaggacg aaaccctggc ctgcggcacg ggtgtggtcg cctccgttct
     8281 ggccgcggcc ggacgccgct ttgtggattc gccggtggat gtgaccgtgc aaagcggcga
     8341 aaccctgcgc gtgtattttt cccgtcagga cggggctttt tcggaaattt atcttgaagg
     8401 gaaagtgaaa atagtctatc a
//
LOCUS       DAOSIM010000069         8384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_7931_length_8384_cov_8.444831, whole genome shotgun sequence.
ACCESSION   DAOSIM010000069 DAOSIM010000000
VERSION     DAOSIM010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8384
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7931_length_8384_cov_8.444831"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1108)
                     /locus_tag="PLR47_04520"
     CDS             complement(116..1108)
                     /locus_tag="PLR47_04520"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOG12238.1"
                     /translation="MFDTQITRMFGVKYPVICGAMMWLCKPKMCAAVSNAGGIGNLTA
                     GNYETADEFRAAIRETRRLTDKPFFVAITLLPSVRITPEHHKMYVKVCAEEKVAGIEF
                     SGTPLDKVAGMEAVEMLKKAGVKMFHKVGALRHALHAEKIGFDGIYAAGIEEGGHPLN
                     DDVTSMVLTPKIAASVKIPVVTVGGIADGRTMAAALALGAEGVMMASRFIATKECEVH
                     DNIKQELIRRQEFDTVIYGKSIGLQGRGLKSKVIEEVLAIEARNGGFEELIPLLSGQK
                     VKQAWETGDVDYAPLMVGQSIGLINDIPTCAELLERMAAQAKEITARTAKLCGN"
     gene            complement(1119..1847)
                     /locus_tag="PLR47_04525"
     CDS             complement(1119..1847)
                     /locus_tag="PLR47_04525"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOG12239.1"
                     /translation="MKLKIGEIAKRSSVRPSTIRYYVGEGLLPKPEKANEKMAYYDEA
                     CIERLRVIQELKERRYFPLYLIKNILRRMDEGLNLTEAETIENAVFRSGQQAENGLIG
                     QAAFLDATGLTEKELAQARRLGILVPFTFERDKPLYDPDDVRAGRDGIKKMADLGMKL
                     QELVFWVELGRQIVEKEMALRRKMVAGKSTRENIAITAELTQVGNFYRDYILRRLFQK
                     QVEQNIQSSLKAAKKRKTGNNGKK"
     gene            complement(2012..2806)
                     /gene="trpA"
                     /locus_tag="PLR47_04530"
     CDS             complement(2012..2806)
                     /gene="trpA"
                     /locus_tag="PLR47_04530"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOG12240.1"
                     /translation="MGRIGKKFEELRAKKEKALIVYATAGDPSIAATRELALSLEKSG
                     ADILEIGVPFSDPTADGPVIQAASQRAIKAGTTLRGVLDLVADIRRASRIPIVLFGYF
                     NPIFAYGAQKFSKAAKDAGVDGILVVDLPPEEAGELRVFTNAAGIDFISLVAPTTGDD
                     RLKAILKAASGFLYCISITGVTGTAAPKTEDIAREISKIRKLTKLPVAVGFGITSAGQ
                     AKAIARIADGVVIGSSVVKLIGENQNGGDGIRAAAAHVKEIKAALL"
     gene            complement(2965..4146)
                     /gene="trpB"
                     /locus_tag="PLR47_04535"
     CDS             complement(2965..4146)
                     /gene="trpB"
                     /locus_tag="PLR47_04535"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOG12241.1"
                     /translation="MKMLPDQKGHFDAFGGRYAAETLMPALLELEEAYAMAQKDKSFE
                     KEFDRYRRDYAGRPTPLYFACRMTQALGGARIYLKREDLNHTGSHKINNTLGQALLAR
                     RMGKKKVIAETGAGQHGVATATVAALFGMECKIFMGEEDIRRQAPNVFRMKILGAEVV
                     PVQSGTATLKDAMNEAMRYWVGSVRDTFYIIGTTAGPHPYPMMVRNFQSVIGREVKKQ
                     TARAEGRNPDVLVACVGGGSNAMGLFYPFRNEKKVKMIGVEAAGRGMNTLEHSASING
                     GKIGVLHGNKTYLLQDENGQVRDAYSIAAGLDYPGVGPEHAYFAQSGRATYVTVTDEE
                     AIGAFLYLSRQEGIIPALESSHAIAHAMKIAPKMKKRRILVICLSGRGDKDARTIMET
                     L"
     gene            complement(4143..4796)
                     /locus_tag="PLR47_04540"
     CDS             complement(4143..4796)
                     /locus_tag="PLR47_04540"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOG12242.1"
                     /translation="MQVKICGITNLEDALTAAENGADAVGFIFHPPSPRFIEPQKAAQ
                     ITKALPDRLVKVGVFVNEKESFVRHTFEHCRLDMIQLHGDESPGYCRQFPKNRILKAL
                     VLQTEEDVRKSEAFDVAAILVDARHNGLYGGTGRTADWDLARKIAGPLILSGGLNEFN
                     VPAALEVVRPAALDINSGAEEKPGKKDSAKIARMMRIIHSASAGETPLNIFIGRDRT"
     gene            complement(4787..5572)
                     /gene="trpC"
                     /locus_tag="PLR47_04545"
     CDS             complement(4787..5572)
                     /gene="trpC"
                     /locus_tag="PLR47_04545"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOG12243.1"
                     /translation="MILDKIVETKKEEVARLKKEATTERLCEAIAALPPCRDFRAALG
                     GADCAVIAEVKCASPSRGRMLQHFDPAAIAKTYEKNGAAAVSVLTDQTYFLGHTSDLT
                     SVRREVRLPVLRKDFIIDPLQVYETRAVGADAILLIVRALGPRLFEFIALSEALGLCP
                     LVEVHTEEELQAALAAKAEMIGINNRNLDTFATDIQTSCRLRKQIPGDRTVVAESALK
                     NRADIEHLMNAGISSFLIGEALMTAPDIGRALRSLLGRESSCR"
     gene            complement(5703..6713)
                     /gene="trpD"
                     /locus_tag="PLR47_04550"
     CDS             complement(5703..6713)
                     /gene="trpD"
                     /locus_tag="PLR47_04550"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOG12244.1"
                     /translation="MIREALDKVVQNIDLPEGDMMAVMEEVMEGKATPAQIGSFITAL
                     RMKGETVEEVTGAARIMRRKAARVNACATTVVDTCGTGGDKLGTFNISTTTAFVAAAA
                     GIVVAKHGNRAVSSGCGSADVLEALGVNISIDPEIVEECLQQIGIGFLFAPRLHGAMK
                     YAIGPRREIGIRTIFNMLGPLTNPAGATAQLLGVYDAKLAEMFAGVLKNMGTKRAFVV
                     HGVDGLDEVTVTGETRVAELKDGIIRTYNLSPKEFVGTTHPLEAIRGGDPEVNARILT
                     DVLSGKPGACRDVVLLNAALAIVAGEKAGNIQEGVKIAADCIDSGRAAKILRALIEMT
                     NG"
     gene            complement(6710..7297)
                     /locus_tag="PLR47_04555"
     CDS             complement(6710..7297)
                     /locus_tag="PLR47_04555"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOG12245.1"
                     /translation="MILMIDHYDSFTFNIVQYLRQMGEDVRVRRNDRITLEEIRRERP
                     EAIFLSPGPCTPRETGVTIDVIQKFYSSVPLMGICLGHQCIGAAFGGEVVRAERIMHG
                     KVSPVEHDGKTIFAGLPQPFAAGRYHSLLVRPESLPPCLEISARTPEGEIMGLRHKNY
                     PVEGIQFHPESVLTPQGKRILRNFLKQIGRKEHGS"
     gene            complement(7294..>8384)
                     /locus_tag="PLR47_04560"
     CDS             complement(7294..>8384)
                     /locus_tag="PLR47_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HOG12246.1"
                     /translation="VPPDDLNLDESVFVISDSLIIFDNVRHTIKVVACAFAEDEDSRA
                     EAYAAACRKIDDLIALLQTPAEYPAPAQKASVVVFESNMTPDRYKAIVEKAKDYIAAG
                     DAIQVVLSQRFSTDCTADPVDLYRALRCVNPSPYLFFLKLDDLTLIGSSPEVMVRLEQ
                     NEVELRPIAGTRPRGKNEQEDRALADELLSDEKERAEHVMLVDLGRNDLGRIAQTGSV
                     QVNQYMVVEKYSHVMHLVSNVRAELDCGRDAFDVLTATFPAGTLSGAPKVRAMQIIDE
                     LEPVRRGVYGGAVGYIGFSGNMDLCITIRTMVIRDGRIFIQAGAGIVHDSRPEAEHME
                     TLHKARGMQKAVELASSGFVLENGRSAL"
BASE COUNT         1579 a         2516 c         2350 g         1939 t
ORIGIN      
        1 gctgatccag ctcatgccgg acagaacgat gggatacttg atgccgacca tttcagtgat
       61 tcgtgttttc atagttgctc cttctatcgt gtagtttggt ttgaaagcct ccggttcagt
      121 ttccgcacag ctttgcggtt cttgcggtga tctctttggc ctgggctgcc attctttcca
      181 gcagctccgc gcaggtgggg atatcgttga tcaggccgat ggactggccg accatcaaag
      241 gcgcgtaatc aacatcgccg gtttcccagg cttgcttgac tttctggccg gacaagagcg
      301 gaatcagctc ttcaaacccg ccgttgcggg cttcgatagc caggacttct tcaatgacct
      361 tggatttcaa tccgcggccc tgcaggccga tggattttcc gtaaatcacc gtgtcaaatt
      421 cctgccggcg gatcagttcc tgcttgatgt tgtcatgcac ttcacattcc ttcgtcgcga
      481 tgaagcggct ggccatcatc acgccctcgg cgcccagcgc caaagcggcg gccatggtcc
      541 ggccgtccgc gatgccgcca acggtgacca cgggaatttt taccgaagcc gcaatcttgg
      601 gtgtcagcac catggaggtg acatcgtcat tcaacgggtg accgccttct tcgatgccgg
      661 cggcatagat tccgtcaaag ccgatttttt ccgcatgcag ggcatggcgc aaagcgccga
      721 ccttatgaaa cattttcacg ccggctttct tgagcatctc gacggcctcc attccggcca
      781 ccttgtccag cggcgtgccg gaaaactcga tgcccgccac tttttcctcg gcgcaaactt
      841 taacatacat cttgtggtgc tccggcgtga tccggaccga gggcagcagc gtgatggcca
      901 caaagaaggg tttatcggtg agccggcgcg tttcccggat cgccgcgcga aattcgtccg
      961 ccgtttcata attgcccgcc gtcagattgc cgatgccgcc ggcattggac accgccgcgc
     1021 acatttttgg tttgcaaagc cacatcatgg ccccgcagat aacgggatac ttgactccga
     1081 acatcctggt gatttgcgtg tcgaacatga aatacctcct atttttttcc gttgtttccg
     1141 gtctttcgct ttttggccgc cttcaggctg ctctgaatat tctgctccac ctgcttctgg
     1201 aaaagacgcc gaagaatgta gtcccggtag aaatttccga cctgcgtcag ttcggcggta
     1261 atggcgatgt tttcccgcgt cgattttccg gcgaccatct tgcggcgaag cgccatctct
     1321 ttctccacga tttgccttcc cagctccacc cagaagacca gctcctgaag cttcatcccc
     1381 agatcggcca tctttttgat gccgtcccgg cccgcgcgga cgtcgtccgg atcatacagg
     1441 ggcttgtccc tttcgaacgt gaacgggacc aggatgccca gacgccgggc ctgcgccaat
     1501 tccttttccg taaggcccgt cgcgtcaaga aaagcggcct ggccgatgag cccgttttcc
     1561 gcctgttggc cggagcggaa cacggcgttt tcgatcgttt cggcttcggt caggttcagg
     1621 ccttcatcca ttcgacgcag aatgtttttg atgaggtaca gaggaaagta gcgtctttcc
     1681 ttcagctcct gaatgacgcg caggcgctcg atgcaggcct cgtcgtaata ggccattttc
     1741 tcattcgctt tttcgggttt gggcaaaagc ccctctccga cgtaatagcg gatggtcgag
     1801 ggccgcacac tgcttctttt cgcgatttcg ccgattttca atttcattca ctgcaccaaa
     1861 cattaaagtt gaggtttaat gttatagtga tattgaagac ggctgtcaag caaattgtca
     1921 aaaagacaaa aaccaagtga aaacaaaaag aaagcccgcc tgctcggctc tttggaatga
     1981 aaatctcgag gcggccggca aagatcattg cttacaacag ggcggctttt atttctttga
     2041 catgcgcggc agccgcccga atcccgtcgc cgccgttttg gttttccccg atgagtttga
     2101 cgacggagct cccgatgaca acgccgtcgg cgattcttgc tatggccttg gcctggccgg
     2161 cgctcgtgat gccgaaaccc acggcgaccg gcagtttggt taatttgcgg atttttgaaa
     2221 tctcgcgggc gatgtcttct gttttcggag cggcggtgcc ggtgacgccc gtgatggaga
     2281 tgcagtaaag aaatcctgaa gcggctttca gaatggcttt caaccggtcg tcgcccgtgg
     2341 tcggcgcgac aagcgaaatg aagtcgattc cggccgcatt cgtaaaaacc ctcagttcgc
     2401 ccgcctcctc cggcggcagg tccacgacga ggatgccgtc cacccccgca tcttttgccg
     2461 ctttggagaa cttctgggcg ccataggcaa agatcggatt aaagtagccg aaaagcacaa
     2521 tgggaatccg ggatgccctg cgaatatcgg ccaccagatc cagaacgcct cgcagtgtgg
     2581 ttcctgcttt gatggcccgc tgggatgcgg cctgaatgac ggggccgtcg gccgtgggat
     2641 cggaaaacgg cacgccgatt tccagaatat ccgctcctga tttttccaga ctcaacgcca
     2701 gttcgcgcgt cgccgcgatc gacggatcgc ccgccgttgc ataaacgatc agcgcctttt
     2761 cttttttggc gcgcagctct tcaaattttt tcccaattct tcccatattt tcctcttttc
     2821 attttcgctc cctccttcct gccgggcggg gactgaaggg aggggaaata attcattttc
     2881 accctcaccc agccctctcc catcaaggga gagggcctcg cagggaacgc tcgcgaccac
     2941 ccatgcgggc ggcaagacgg ttccctacag cgtttccatg atcgtccggg cgtccttgtc
     3001 gccgcggccg gaaaggcaga tgaccaaaat ccggcgcttc ttcattttcg gagcgatttt
     3061 catggcgtgg gcaatcgcgt gcgaactttc cagcgccgga atgatgcctt cctgccgcga
     3121 caaatacaaa aacgctccga tcgcttcctc atccgtcacg gtgacatacg tggcgcggcc
     3181 ggattgtgca aaataggcgt gctccgggcc cacgccgggg taatccagtc ccgccgcgat
     3241 ggagtaggcg tcgcgaacct ggccgttttc atcctgcaga aggtaggtct tgttgccgtg
     3301 caggacaccg atttttccgc cgttgatgct tgccgaatgc tccagcgtat tcatgccgcg
     3361 gccggctgct tccacgccga tcatcttcac cttcttttca ttgcggaagg gataaaacag
     3421 gcccatggcg ttggagccgc cgcccacgca ggccaccaga acatcgggat tccgtccttc
     3481 cgctcttgcc gtctgctttt tgacttctcg cccgatgacc gactggaaat tgcgcaccat
     3541 catcggatag gggtgcggcc cggcggttgt tccgatgatg taaaacgtgt cgcggacact
     3601 tcccacccaa tagcgcatgg cttcgttcat cgcgtccttg agcgtggccg tgccgctttg
     3661 gaccggaacc acctcggcgc ccagaatctt catgcgaaag acgttgggcg cctggcggcg
     3721 gatgtcttct tctcccatga agattttgca ttccattccg aaaagcgcgg ccacggttgc
     3781 ggtcgccacg ccgtgctggc ccgcgccggt ttcggcgatg acttttttct tgcccatgcg
     3841 ccgggccaga agcgcctgcc ccagcgtgtt gttgatcttg tgcgagccgg tgtggttgag
     3901 atcttcgcgt ttgagataga ttctggcgcc gccaagcgcc tgagtcatcc ggcaagcaaa
     3961 ataaagcggc gtcggccggc ctgcgtagtc tcgccggtac cggtcgaatt ccttctcaaa
     4021 ggatttgtct ttttgcgcca tggcgtaagc ttcttcgagc tccagcagag cgggcatgag
     4081 cgtctcggcc gcatagcggc ctccgaaggc atcaaagtgt cctttttgat ccggcagcat
     4141 tttcatgttc tgtcccttcc aataaaaata ttcagcggtg tttcgccggc tgacgcggaa
     4201 tgaatgatcc gcatcatgcg cgcgattttt gcggagtctt ttttgcccgg cttctcttcc
     4261 gcgccgctgt tgatgtccag tgcggcaggc cggaccacct caagggctgc aggaacattg
     4321 aattcgttca agccgcccga taaaatcagc ggcccggcaa tcttcctggc caggtcccag
     4381 tcggccgtcc tgcccgtgcc gccgtaaagc ccgttgtggc gcgcatcaac gagaatcgcg
     4441 gcgacatcaa atgcttcaga cttccggaca tcttcttcgg tttgaagaac cagcgccttg
     4501 agaatccggt tttttggaaa ttgccggcag tagccggggg attcgtcgcc gtggagctga
     4561 atcatgtcga ggcggcagtg ctcaaacgtg tgccggacaa aggattcttt ttcattgacg
     4621 aagacgccga ccttcaccag ccggtccggc agcgcttttg tgatctgtgc cgccttttgc
     4681 ggctcgatga agcgagggga aggcggatgg aaaataaaac cgaccgcgtc tgcgccgttt
     4741 tcagccgcgg tgagtgcatc ttcaaggttt gtgatgccgc atatttttac ctgcatgacg
     4801 attccctccc cagaagcgag cgcagtgcac ggccgatgtc cggagcggtc atcagcgctt
     4861 cgccgatgag aaaagaagaa atgcccgcgt tcatcaggtg ttcgatatcg gcgcggtttt
     4921 tcagagcgct ttccgcgacg accgtgcggt cgccgggaat ctgtttcctg agtcggcaac
     4981 tggtctgaat atccgtcgcg aacgtatcga gattgcggtt gttgatgccg atcatctccg
     5041 cttttgccgc gagtgccgcc tgcagctcct cctctgtgtg aacctcgacc agcgggcaca
     5101 ggcccagtgc ttcggaaagg gcgatgaatt caaaaagccg cggtcccagc gcgcggacga
     5161 tcagcagaat cgcgtcggcg ccgacggctc tcgtttcgta aacctgaagc ggatcgatga
     5221 taaaatcctt gcgcaggacg ggaagacgaa cttcacgccg aacgctcgtc aaatcgcttg
     5281 tgtggcccaa aaaataggtt tggtcggtca aaacggaaac ggcggccgcg ccatttttct
     5341 catacgtttt tgcgatcgcc gccggatcga aatgctgcaa catgcgtccg cgggaaggcg
     5401 aggcgcattt cacttcggcg atgacggcgc agtcggcacc gccgagagcc gcgcggaaat
     5461 cccggcaggg cggcagcgct gcgatggctt cgcaaagacg ctcagtcgtc gcctcctttt
     5521 tgagacgggc cacttcctct tttttggttt caacgatctt gtctaaaatc atgtcgtcct
     5581 ctttttttct cccctttttt caccctctcc ctaaccctct cccatcaagg gagagggaat
     5641 gaacctgcaa ttcatgatcg ccgccgctaa accctaacat tctcccgtca agggagaggg
     5701 aattaaccgt tcgtcatctc gatgagcgcc cgcagtattt ttgccgcccg gccgctgtcg
     5761 atgcagtcgg cggcgatttt aacgccctcc tgaatattgc ctgctttctc accggcgaca
     5821 atcgccagcg ccgcgttcag taaaacgaca tcccggcagg cgcccggctt tccggagagc
     5881 acgtccgtca ggattcgggc gttgacctcc ggatcgcctc cgcggatcgc ctcgagcggg
     5941 tgagtggttc cgacgaactc cttcggggaa aggttgtagg tgcggatgat gccgtctttg
     6001 agctccgcca cgcgggtttc cccggtgacc gtcacttcgt cgaggccgtc gacgccgtgc
     6061 accacaaaag cccgcttcgt ccccatgttt ttcaaaacgc cggcgaacat ttcggcaagc
     6121 tttgcgtcgt aaacgcccag cagctgcgcg gtcgcgccgg ccgggttggt gagcggcccc
     6181 agcatgttga aaatcgtgcg gatgccgatt tcacggcgcg ggccgatggc gtacttcatc
     6241 gcgccgtgca gcctcggcgc gaacagaaag ccgatgccga tctgctggag acactcttcg
     6301 acgatttccg gatcgatgct gatattgacg ccgagagcct ccagcacgtc ggcgctgccg
     6361 cacccgctgg agacggcccg gttgccgtgt ttggcgacca cgatgcccgc cgccgccgcg
     6421 acaaaggccg tcgtggtgga aatgttgaac gtgcccagct tgtcgcctcc ggtgccgcag
     6481 gtatcgacga cggttgtcgc gcaggcgttc acgcgcgcgg ccttccggcg catgatgcgc
     6541 gccgcgccgg tcacttcttc cacggtttcg cccttcatgc ggagcgcggt gatgaacgag
     6601 ccgatctgcg cgggagtggc tttgccctcc atcacttctt ccatgacggc catcatatcc
     6661 ccctccggca gatcgatgtt ttgtactacc ttgtccaaag cttctctgat catgatccat
     6721 gctcctttcg gccgatctgt tttaaaaaat ttcgaaggat tcttttcccc tgcggcgtaa
     6781 ggacggattc cggatgaaac tgaattcctt ccacgggata atttttgtgc cgcagtccca
     6841 tgatttcacc ctcgggggtc cgggcgctga tttcaaggca gggcggcagg gattccggcc
     6901 gcaccagcag ggaatgatac cgccccgcgg caaagggttg gggcagcccc gcaaaaatcg
     6961 ttttgccgtc gtgctccacg ggcgatacct tgccgtgcat gatgcgttcg gcgcgaacga
     7021 cttctccgcc gaacgcggca ccgatgcact ggtgtcccag gcagatgccc atcagcggca
     7081 cgctcgaata aaatttctga atcacgtcga tggtgacgcc cgtttcccgg ggcgtgcatg
     7141 ggccgggcga taaaaaaatt gcttcgggtc tctcgcgccg gatttcttcg agggtgatcc
     7201 ggtcgttgcg ccggacccgg acgtcctccc ccatctgcct caggtattga acgatgttga
     7261 acgtaaagga atcataatgg tcgatcatca gaatcataga gcgctcctgc cgttttcgag
     7321 aacgaatccg cttgaggcga gctccacggc cttctgcatg ccccgcgcct tgtggagcgt
     7381 ttccatgtgt tcggcttcgg gcctggaatc atggacaatg cccgcgccgg cctgaatgaa
     7441 aatccttccg tcccgaataa ccatcgtgcg gatggtgatg cacagatcca tgttgccgct
     7501 gaagccgata tagccgaccg cgccgccgta cacgccccgc cgcacgggtt cgagttcgtc
     7561 gatgatctgc atggcccgga ctttgggcgc gccggagagt gttcccgccg gaaacgtggc
     7621 ggtcagcacg tcaaaggcgt ccctgccgca atcgagctcg gcccgcacat tggataccag
     7681 gtgcatgaca tgcgaatatt tttcgaccac catgtactgg ttgacctgca ccgacccggt
     7741 ttgggcgatc ctgccaaggt cgtttctgcc cagatcgacc agcatgacgt gttcggcccg
     7801 ctccttttca tcggagagca gctcgtcggc cagcgcgcgg tcctcctgct cgttttttcc
     7861 gcgcggccgt gtgccggcaa tcggcctcag ctccacttcg ttttgctcca ggcgcaccat
     7921 gacttccggc gaagagccga tcagggtcag atcatccagc ttgaggaaaa acagataggg
     7981 cgacgggttc acgcagcgga gcgcccggta aagatcgacc ggatcggccg tgcagtccgt
     8041 ggaaaaacgc tgggagagca ccacctgaat ggcgtctccc gcggcgatgt agtctttcgc
     8101 tttctccacg atggccttgt accggtcggg cgtcatattg gattcaaaaa ccacgacgga
     8161 agctttttgg gcgggagccg gatattctgc cggcgtctgc aacagcgcaa tcagatcatc
     8221 gatctttcgg cacgcggccg cgtacgcttc cgcgcgggaa tcctcgtctt ccgcaaaggc
     8281 gcaggcgacg accttgatcg tatggcgcac attgtcaaag ataatcaggg aatcgctgat
     8341 gacaaaaacg gattcgtcca gattcaggtc atcgggcggc acat
//
LOCUS       DAOSIM010000070         8312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_8017_length_8312_cov_8.295991, whole genome shotgun sequence.
ACCESSION   DAOSIM010000070 DAOSIM010000000
VERSION     DAOSIM010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8017_length_8312_cov_8.295991"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLR47_04565"
     CDS             complement(<1..248)
                     /locus_tag="PLR47_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12247.1"
                     /translation="MFMDKRFNDLILAAIKQRISDLHITGGYPLIFRRDGVIHSDKNV
                     KWTHTEIDNMVKEMLTDRQLFTLRKRWSVDFAMSLQEVR"
     gene            528..1115
                     /locus_tag="PLR47_04570"
     CDS             528..1115
                     /locus_tag="PLR47_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12248.1"
                     /translation="MKCKRLWVIAAALGMMLVFPALEKPAFAHEKIVDFILKVHPDGP
                     ASAIPEDFLRTGSDDGYGYAHEGYIDGFKIICRSKDGIVKSVHAQKQFNTGDLLAEHT
                     YYNILGSVLSRLMKKYNGTLVNEKGRGESTDKVPYDYGEYCYYTALKGAGKTIKTRDY
                     KLGFWYDLGVWKRCDNYSYNGENPGNVIIEFRPGN"
     gene            1235..2326
                     /locus_tag="PLR47_04575"
     CDS             1235..2326
                     /locus_tag="PLR47_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12249.1"
                     /translation="MKKWTLFLVMLFLAASVFPVYAQKKITAKGMAANQRNIDIACSR
                     ALDEAQRNAVEQATGVMITSTTNVENFQVKMDRILSESKGFINAYKVLSEKKAGTDCE
                     VVIEADVSTGKLRDRMMAVNLIMARKSKPRVMLVFKDAVAEAVMAKYLLGHNFKLVDA
                     DVSSKNREYDRLKDASEARDIRGLAQHYGAEVIIVGTVSADSKSSTIYNIEMNKNTVV
                     VTGKVINGDTGEIITTASETKSDNNMKGDYKALTEETSSKLISKLVDDVLENWSKELA
                     NTASVKLIVTGLDEETLEDLKAELGEEIKGLKEVNERFYAGGKAEFDLEVEGNAQDLA
                     RDLRKITVNKRKIKIKSRTQNRVEAVLLP"
     gene            2365..4350
                     /locus_tag="PLR47_04580"
     CDS             2365..4350
                     /locus_tag="PLR47_04580"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOG12250.1"
                     /translation="MKSRKTYMAIASAVVLLVLAACVGTNESLKQGQEFAKQNRWDEA
                     MIYFEKAVSEEPDNSEFKSELTRAKQEVAKARLARAKQSHAAAGQNFMALERLSKEAD
                     EILKLDPNDPEVRSFSATVNGQVNNLKGNLKNLYQQAQADMQKEDWLAAIDKLKQVNA
                     IFPNYEDTGNRLRRSLSEGTRAMYQQGTKLGKQEDWKGAAESFKAVMDVNPKYLDAAQ
                     QYANAVKNDNPAYFAAEAAKAEGAGKMDRAIAMYEKALEYPQADPKLNDSLAALKAKS
                     SQIYLNDSIELFKQERLYGSFKKLDLAGKYAPALRSEPAYAEHANRLCEALMKRANKF
                     AEKELWGNALVWLQLVDRINPEYEGLFQKMNEVKDPISRRVRKSIAVFDFGSPSTDKD
                     AGKIAANRLLAYLHQNASVDLRIIERENLQSILREMQLSQTGLVDIKTAQSVGKMRGI
                     DTFIMGDVLQFSAKYTDVATTNQVRVLINEEDVPNPDWSDWRILNPKPSAEDLKNAPA
                     RTIKKPNYQFLPNKQGTARINAMLEISFKLVDTQSGEVNANTINGKLVKEDKYQEGLN
                     IAGIKADPLELPTEGEVLDQLAKEKIAEMGRDVLKHFQSLEVEYFNKGQDLQKRRNYE
                     AATERYTDAIFDEKLKTISTPISQKSAELIELINEFN"
     gene            4365..6626
                     /locus_tag="PLR47_04585"
     CDS             4365..6626
                     /locus_tag="PLR47_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12251.1"
                     /translation="MLKKILAGAAVLLLLLLAETGILHAQELKLSVFNFGTANIEASG
                     LGTSVTNALISNMSRNPSISILDRKDLETFLNMNDLQQNDQLDNVVNIGSRLGLDFIV
                     VGSVAKRGSAITVKCGLIQIDKRKEVYSTHVQAQGESALSAEIAKLASLITSVLTKSG
                     AGPESESAQGGGGPGAAAPVNFRGVPGNKKIILRWQVVPGFAAAGYEVYRAQNANGPF
                     AMLGQTDKTEFADQNVDNDISYFYKVRAYDRLSRLSGYTPVVSARTDFAPNPPIILKT
                     EGRAKSMLIVWAPNPSKSPDPSPLVGYKIYRSAENEEGSYQEVNKLLLGDCADSGDGK
                     VYYRATGLPDGQTFYYRLAAYNAKGIESELSHPVRGTTLPAVTSLKAAGQMIREVRLT
                     WTGISSPFTAAYNIYRSAKSDGGFLKIKRISASEAGPRYEYSDTEGLADNTPYYYTIT
                     AEDDLKTETSPSAAVAASTRDIPPRPENFAARSGLVKKVELTWQASPQPEVEGYYIFG
                     SVQKAGNYELLKKIPGRENSRYVDESRGFNALEDGTVYYYMMTAYNKVDAQSKQAQAG
                     ATTKPRPKKPAGLKGTSLRVKEAPLAWQANPEKDIRVYLIQRAAGDGDSFSGIAEAGA
                     TLFTDTNLKDGETYRYRIQAKDADNLLSDFSDVVAVTTKPRPRPPENVQGKFESGKAL
                     LSWTPNKEADISHYVVYEKKFIGAEKVAEAKTASYSDGSLAPGKSRNYVVTAVDKSGL
                     ESDASAEWSVSAK"
     gene            6733..7134
                     /locus_tag="PLR47_04590"
     CDS             6733..7134
                     /locus_tag="PLR47_04590"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOG12252.1"
                     /translation="MSDLLAYLSPSWRDEALQRLQSELPPEKMNNVTTSMSNIYKNCP
                     GGEEQFLFVECRDGKVTRVETGSGEPPQAEFRIIGDYETFAKISRAELGSQKALMTGK
                     LKLKGNLARALKLAAVADRLNKVLAKIPTQY"
     gene            7150..>8312
                     /locus_tag="PLR47_04595"
     CDS             7150..>8312
                     /locus_tag="PLR47_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOG12253.1"
                     /translation="MTRILDPTDKYYIDNPGRVKAIQAEMARRGIDVYLGSRIRTMSF
                     VLDTFCPWRSYVIIPPEGQPAFFTFVVDALRIADETWIDSDHVRAYGPMGGMDQVSAI
                     TDFIKEELGIRKGRLGFEDGISTYTAEGNLSHWEYTHFKDALAGFEWENSHDIVDGLS
                     LIKDKGTIERFRTASLIVDEGHKAARAALINGGFKGMTETVIAGIAALAMRKAGSVSE
                     WNFAGLNEISSGFRTGLGACTPPTTKEIGAGEPLMLDFHAMFKLALGDHSHNYLIGPA
                     TKRQRWHADNFVAIIQKVIDNYRAGTTPGTLAAIMMDFAESRGCGDFIQPACEHGIGL
                     FGDEWRIGAVVNPALPYWTDQDHVYQENEMVVCAMQYAAPEENIGFRYENDIV"
BASE COUNT         2150 a         2359 c         2341 g         1462 t
ORIGIN      
        1 cgcacctcct gcaaagacat cgcgaaatcg acggaccatc ttttccgcaa agtgaaaagc
       61 tggcgatccg tcagcatttc cttgaccata ttgtcgattt ccgtatgcgt ccatttcacg
      121 ttcttatcgg aatgaatcac gccgtccctg cggaagatca gcggataccc tcccgtgatg
      181 tgcagatccg aaattctctg cttgatggcc gcaagaatca aatcattgaa gcgtttgtcc
      241 ataaacatcc cgaccgtttg gcctccattc ggctgccggg ggagcggctc ccgccccccg
      301 gacccgtaac cgctgaaacc atagaagaaa agccggcccc cgtcaagcgg aaaagcggtc
      361 gaaaaacata cagatattca tcgcccttca cggcaaaacg tcctgatgaa cgcgctgtgc
      421 cgggtcgcct cacgtgcgcc ggaacgcaag ttttgtcttg acatatgaag gtggcggttt
      481 tacaaattcg tgaagcaccc cggaaaggga gaaaagagga aaaaacaatg aaatgcaaaa
      541 gactttgggt gatcgcggcg gcgctgggaa tgatgctcgt tttcccggcg ctcgaaaaac
      601 cggccttcgc ccatgaaaaa atcgttgatt tcatcctcaa agtgcatccg gacgggccgg
      661 cttcggccat tcccgaagac ttcctgagaa cgggcagcga cgacggctac ggctacgcgc
      721 atgaaggcta tatcgacggc tttaaaatca tctgccgttc caaagacggc atcgtgaaat
      781 ccgtgcacgc gcaaaagcag ttcaacacgg gcgacctctt ggccgagcac acctattaca
      841 acatcctggg gagcgttctg tccaggctga tgaaaaaata caacggcacg ctcgtcaacg
      901 agaaggggcg gggggaaagc accgacaagg tgccgtacga ctacggggaa tactgctact
      961 atacggcgct gaaaggagcg ggaaagacga tcaagaccag ggactacaag ctgggattct
     1021 ggtatgacct cggcgtctgg aagcggtgcg acaactacag ctacaacgga gaaaatccgg
     1081 ggaacgtcat cattgaattc cggccgggaa actgacgccg gatccggaaa tctccggcct
     1141 gctttccaat gctttcaact tgacacccat ggtcaaattc agtatgtaga agcgtaatta
     1201 ctttaaaaat ttctgaacac aagggatgaa tttcatgaaa aaatggacgc tctttcttgt
     1261 gatgctgttt ctggcggctt cggtttttcc cgtttacgcc cagaaaaaaa tcaccgccaa
     1321 aggaatggcg gccaaccaga gaaacatcga catcgcctgc agccgggctc tggatgaagc
     1381 gcagcgcaac gccgtggagc aggcgacggg cgtgatgatt accagcacca ccaacgtgga
     1441 aaatttccag gtgaagatgg accgcattct ttcggaatcc aagggcttca tcaacgccta
     1501 taaagtcctg tccgaaaaga aagccggaac ggactgcgaa gtggtcatcg aggcggatgt
     1561 gagcacggga aagcttcgcg acaggatgat ggccgtcaat ctgatcatgg cgcgcaaatc
     1621 caagccccgg gtcatgctgg tgttcaagga cgccgtggcg gaagccgtca tggccaaata
     1681 tctgctcggg cacaacttca agctggtgga cgcggatgtt tccagcaaaa acagggagta
     1741 tgaccgcctg aaggacgctt cggaggccag agacatccgc gggctggcgc agcactacgg
     1801 cgcggaggtg atcatcgtcg gaacggtgag cgcggacagc aagtcctcca ccatttacaa
     1861 catcgagatg aacaagaaca cggtggtggt caccggcaag gtgatcaacg gggacacggg
     1921 ggaaatcatc accaccgcca gcgaaaccaa atccgacaac aacatgaagg gggactacaa
     1981 agccttaacg gaagaaacct cctccaagct gatttcaaag cttgtggatg acgtattgga
     2041 aaactggtcg aaggaactgg ccaacaccgc ctcggtcaag ctgatcgtca cgggactgga
     2101 cgaagaaacg cttgaagatc tcaaggccga actgggggaa gaaatcaagg gcctcaagga
     2161 agtcaacgag cgtttctacg ccggaggcaa agcggaattt gacctggaag tggaaggcaa
     2221 cgcgcaggat ctggcccgcg atcttcggaa aataaccgtc aacaagcgta aaatcaaaat
     2281 caaaagcaga acccaaaaca gagttgaggc ggttctattg ccttaaggat cgatattcgg
     2341 aaacttttga gtggggagga ttgaatgaaa agtagaaaaa cctacatggc gattgcatcc
     2401 gcggtggtcc ttttggtcct ggcagcctgc gtgggaacca acgaaagcct caagcaggga
     2461 caggagtttg ccaaacaaaa ccgctgggat gaagcaatga tttattttga aaaggcggta
     2521 agcgaagaac cggacaactc tgaattcaaa agcgaactga cccgggcaaa gcaggaagta
     2581 gccaaggcca gactggcgag ggccaaacag tcccacgcgg cagccggaca aaatttcatg
     2641 gcgctggagc ggctgtccaa agaagccgac gaaatcctga aactcgaccc gaacgacccg
     2701 gaagtcaggt ctttttccgc aacggtcaac ggacaggtca acaacctgaa gggcaacctg
     2761 aaaaacctgt atcagcaggc ccaggccgac atgcagaagg aagactggct ggcggccatc
     2821 gacaagctga agcaggtcaa cgccatcttc ccgaattatg aagatacggg caaccggctc
     2881 cgtcgctccc tgtccgaggg caccagggcc atgtaccagc agggcaccaa actgggaaaa
     2941 caggaagact ggaagggcgc cgcggaaagc ttcaaggcgg tcatggacgt caatccgaaa
     3001 tatctggatg cggcccagca gtacgcgaat gccgtcaaaa acgacaatcc cgcctacttc
     3061 gcggcggaag cggcaaaagc cgagggcgcc gggaagatgg accgggccat tgccatgtat
     3121 gaaaaagccc tggagtatcc gcaggccgac cccaaactga acgacagtct ggcggcgctg
     3181 aaagcaaaat cgagccagat ttacctgaat gactccatcg aacttttcaa acaggaacgg
     3241 ctttacggct cgtttaagaa gctcgatctg gccggcaagt atgcaccggc cctgcgcagc
     3301 gaacccgctt acgcggaaca cgcaaaccgc ctttgcgaag ccctgatgaa aagggccaat
     3361 aaatttgcgg aaaaggaact gtggggcaac gcgctggtct ggctgcagct tgtcgacagg
     3421 atcaacccgg agtacgaagg gttgttccag aaaatgaacg aagtgaagga tcccatctcg
     3481 cggcgcgtcc ggaagtcgat cgccgttttt gatttcggct cccccagcac ggacaaagat
     3541 gccggcaaaa tagccgccaa ccggctcctg gcctatctgc atcagaacgc cagcgtcgat
     3601 ctgcgcatca tcgagcggga aaatctgcaa tccattctcc gggaaatgca gctcagccag
     3661 acggggctgg tggatatcaa aacggcgcag agcgtcggca aaatgcgcgg catcgacaca
     3721 ttcatcatgg gagacgtgct gcagttttcc gcaaagtaca cggacgttgc gacgacgaat
     3781 caggtcagag tcctgatcaa tgaagaggac gtccccaatc ctgactggtc ggattggcgc
     3841 atattaaatc ccaaaccttc ggcggaggat cttaaaaacg ctcccgcccg gaccattaaa
     3901 aaaccgaact accagttcct gcccaataag cagggcaccg ccagaatcaa cgccatgctg
     3961 gaaatatcct tcaagctggt ggatacccaa tcgggcgaag tcaacgccaa cacgatcaac
     4021 ggaaaactgg tgaaagaaga caaataccag gaaggcttga atatcgccgg cattaaggct
     4081 gatcctctgg agctgccgac ggaaggcgaa gtgctggatc aattggccaa agagaaaatt
     4141 gcggaaatgg gacgcgatgt gctgaaacac tttcaaagcc tggaagtgga atatttcaac
     4201 aaggggcagg acctgcagaa acgccgcaat tacgaggcgg ccacggaacg gtacacggat
     4261 gccattttcg atgaaaaatt gaaaaccatc tccacgccga tttcacagaa atcggcggag
     4321 ctgattgagc tgatcaacga attcaattaa tacggagacc gatcttgctg aagaaaattc
     4381 ttgccggcgc tgctgtattg ctgctgttgc tccttgcgga aacgggaatt ctccacgcac
     4441 aggaattaaa gctgtccgtc ttcaatttcg gaacagccaa cattgaagcg tcgggcctgg
     4501 gaaccagcgt caccaacgcg ctgatcagca acatgagcag aaatccctcc atttccattc
     4561 ttgaccgcaa ggatctggaa acatttctga acatgaacga ccttcagcag aatgatcaac
     4621 tggacaacgt cgtcaacatc ggctcgaggc tgggcctcga ttttatcgtc gtcggctccg
     4681 tcgccaagcg cggatccgcc atcaccgtca aatgcgggct gatccagatc gataaaagaa
     4741 aagaagttta cagcacccac gttcaggcgc agggcgaatc ggccctgtcc gcggagatcg
     4801 caaagctcgc ctccctgatc acctcggtgc tgaccaagag cggcgcggga cccgaaagcg
     4861 aatccgcgca aggcggagga ggaccgggcg ccgccgcccc cgtcaatttc aggggcgtcc
     4921 cgggcaacaa aaaaatcatc ctgcgctggc aggtcgtccc ggggttcgcg gccgccggat
     4981 atgaagtgta ccgcgcccag aatgcgaacg gcccgtttgc catgctgggc cagacggata
     5041 aaacggaatt cgccgatcaa aacgtggaca acgacatttc ctatttttac aaggtccggg
     5101 cctacgaccg gctgagccgg cttagcggct acacgcccgt cgtttccgcc cgcacggatt
     5161 tcgcgcccaa tccgccgatt attctgaaaa cggaaggccg ggccaagagc atgctgattg
     5221 tctgggcgcc caatccctcc aaatcaccgg atccctcgcc gcttgtcggg tataaaattt
     5281 accgctccgc cgaaaacgaa gagggatctt accaggaagt gaacaaactg ctgcttggcg
     5341 attgcgccga cagcggcgac gggaaggtct attaccgcgc gacgggactg cccgacgggc
     5401 agacgtttta ctaccgcctg gccgcttaca acgccaaggg cattgaaagc gaattaagcc
     5461 accccgtcag gggaacgacg cttccggcgg tgacgtccct caaggccgcc ggccagatga
     5521 tccgtgaagt ccggctgacc tggacgggca tttcgtcgcc tttcaccgcc gcctataata
     5581 tttaccgctc cgccaaaagc gacggcggct ttttgaaaat caaaagaatc agcgcttccg
     5641 aggccggccc ccggtatgag tattccgaca cggaaggcct ggccgacaac accccctatt
     5701 attacacgat cacggcggaa gatgatttga aaacggaaac aagcccgtcg gcggccgtcg
     5761 ccgcgtccac ccgggacatt ccgccccggc cggaaaactt cgccgcccga agcggtctgg
     5821 tcaagaaagt cgagctgacc tggcaggcgt cgccgcagcc ggaagtggaa ggctactata
     5881 tattcgggtc ggtccagaaa gccgggaact atgagctcct caaaaagatc ccggggcggg
     5941 aaaacagccg ctacgtggac gagtcccgcg gtttcaacgc actggaagac gggaccgtct
     6001 attattacat gatgaccgcc tacaacaagg tggacgcgca aagcaagcag gctcaggccg
     6061 gcgccacgac caagccccgg ccgaaaaaac cggccggatt gaaaggcact tccctgcgcg
     6121 tcaaggaagc gcccctggcc tggcaggcca atccggaaaa agacattcgg gtttacctga
     6181 ttcagcgggc cgcgggagac ggcgacagct tctccggcat cgccgaggcc ggcgcgaccc
     6241 tgtttacgga taccaatctg aaggacggcg aaacctaccg ctaccggatc caggccaaag
     6301 acgccgacaa cctgctttcg gacttttccg acgtcgttgc cgtgaccacc aaaccccggc
     6361 cgcggccgcc cgagaacgtg caggggaaat tcgaatccgg aaaagctctc ctgtcctgga
     6421 cgcccaacaa ggaagcggac atttcccatt acgtcgttta cgaaaagaag ttcatcgggg
     6481 cggaaaaagt tgcagaagcg aaaaccgctt cctattcgga cggttccctg gccccgggaa
     6541 aaagcaggaa ttacgtcgtc accgccgttg ataaaagcgg cctggaaagc gacgcgagcg
     6601 cggaatggtc cgtttcagcc aagtaacggg ggccgcttcg cgccgcaatc agatgtctcg
     6661 cagcccgcaa cacccttcgg ccggggctgt tttccgctcc ggccgccgga aaacatcaaa
     6721 ggaggaatca acatgtcgga tttactggcc tatttaagcc cgtcctggcg cgatgaggcg
     6781 cttcaaagac ttcagtcgga actgcccccg gaaaaaatga acaacgtcac cacctccatg
     6841 tccaacattt ataaaaactg ccccggcggc gaggagcagt tcctttttgt ggaatgcagg
     6901 gacgggaaag tcacccgcgt agaaacgggc tcgggcgaac cgccgcaggc ggagttccgg
     6961 atcatcgggg attacgagac ttttgcgaaa atttcccgcg cggagctggg ctcgcaaaag
     7021 gcgctcatga ccggcaagct caagctcaag ggcaacctgg cccgcgcgct caagctggcc
     7081 gccgtcgccg accgcctcaa caaggtgctc gcgaagattc caacacagta ttagaaggga
     7141 gatcgcatca tgaccagaat actggaccct accgataaat attacatcga caaccccggc
     7201 cgggtcaaag cgatccaggc cgaaatggcg cggcgcggca tcgacgttta tctcggctcg
     7261 cgcatccgca ccatgagctt tgtgctggat acgttttgcc cctggcgcag ctatgtgatc
     7321 attccgcccg aaggccagcc cgccttcttt acgtttgtcg tcgacgccct gcgcattgcc
     7381 gacgaaacct ggatcgattc cgaccacgtc cgcgcctacg gcccgatggg cggcatggac
     7441 caggtgtcgg caatcacgga cttcatcaaa gaagagctcg gcatccggaa ggggcgcctg
     7501 ggctttgaag acggcatttc cacctacacg gccgagggaa atctctcgca ctgggagtac
     7561 acgcatttca aggacgcgct ggccggtttt gaatgggaaa actcgcacga catcgtggat
     7621 ggcctgtcgc tcattaagga caagggcacg atcgagcgct tccgcaccgc gtcgctgatc
     7681 gtcgatgaag gccacaaggc ggcccgcgcc gcgcttataa acggcgggtt caagggcatg
     7741 acggaaaccg tgatcgccgg catcgccgcg ctggcgatgc gcaaggcggg cagcgtttcc
     7801 gaatggaact ttgcggggct caatgaaatt tccagcggtt tccgcacggg actgggcgcc
     7861 tgcacgccgc ccacgaccaa ggaaatcggc gcgggcgagc ccctgatgct ggattttcac
     7921 gccatgttca aactggcgct gggcgatcat tcgcacaact acctcatcgg gccggcgacc
     7981 aaacgacagc ggtggcatgc ggacaatttt gtcgccatca ttcaaaaagt gatcgacaac
     8041 taccgggccg gaacgacgcc cggcacgctg gccgccatca tgatggactt tgccgagtcg
     8101 cgcggctgcg gcgatttcat ccagcccgcc tgcgagcacg gcatcgggct tttcggcgac
     8161 gagtggcgca tcggcgccgt cgtcaatccc gcgctgccct actggacgga tcaggaccac
     8221 gtctaccagg aaaacgaaat ggtcgtctgc gcgatgcagt acgccgcgcc ggaggagaac
     8281 atcggcttcc gctacgaaaa cgacatcgtc at
//
LOCUS       DAOSIM010000071         8298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_8042_length_8298_cov_8.770715, whole genome shotgun sequence.
ACCESSION   DAOSIM010000071 DAOSIM010000000
VERSION     DAOSIM010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8042_length_8298_cov_8.770715"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1753)
                     /locus_tag="PLR47_04600"
     CDS             complement(56..1753)
                     /locus_tag="PLR47_04600"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOG12254.1"
                     /translation="MHKRIAYVVVFGCLLLFPSAAQAGKGRVNIAVSPVPPALTTSVK
                     FVEPSGNNILDADETGKLVITVTNSGKGDAFDCRALVDTAKRIRGLRFEREVFLGTIP
                     AGETIVKEIDLKAEEEMATDQAAFTITVKEANGFDPAPVKLVFHTKAFAEPKLVVADI
                     GINDAGGNGRIEPMEMVELTVRVQNIGRGDARGVVADVKTGSNVFIGGDAQTHFELGA
                     LPSGRFKDFKFMFYTNTRIANGETIPIAVALTEARPRFNKESQLALVMNAPQKKIEVV
                     QVAGQDSQKGEDIAIVGGLSVDVDTNIPVGQKAGKYDVAVVIGNRIYGQGIPEVAYAE
                     RDARIMKEYLVRTMGFDEGNIIYAENTTLSKLNEIFGTEREPKGRLYNNIKPNRSHVF
                     VYYVGHGAPDLESGDAFFVPVDGNPEYIRAGGYRLQTFYDNLSKLPAKKITVVLDACF
                     SGNTDKGMLFKGISPAMVKVKKEYRGPAGAVVITSASADQVSAWYPEKRHSLFTYYFL
                     KGLQGEADQDKNGKITVGELSEFLKENVPYMARKLKGLDQQPVIMGNPDDVIAVLKK"
     gene            complement(1812..2024)
                     /locus_tag="PLR47_04605"
     CDS             complement(1812..2024)
                     /locus_tag="PLR47_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOG12255.1"
                     /translation="MKKFTLQYWKDDEWYVGRLKEVPGVFSQGETLEELEENIRDAYR
                     LMMEDDTDYIPHVHAPVHQKEIGVDI"
     gene            complement(2415..3422)
                     /locus_tag="PLR47_04610"
     CDS             complement(2415..3422)
                     /locus_tag="PLR47_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
                     /protein_id="HOG12256.1"
                     /translation="MMSAGDLYRQIADRDNIRLAFLKAARGKQARRDVIDFRKNFEEN
                     IEKIHGQLEDRNPDIGHYRYFQVYDPKKRLICAASFPERVLHHAIMNICEPVLESYAI
                     YDSYACRKGKGGQKAILRAQEFAGRFDWFLKLDVRKYFDSIDHAILLGALARRFREKD
                     ILLLFEKIMSSYEVETDKGLPIGNLISQHLANFYLSVFDHWIKEERKVRGYVRYMDDF
                     LIFDDDRDKLMHVELPAIKDFLQARLSLTVKPDIQMNRCARGIPFLGYRVFPQKILLS
                     PRSKRRFVNKFRLYEGKQMNGEWSVDELVRHMDSLLEFTRFADSDGFRRNVISRFGVL
                     S"
     gene            complement(3419..3901)
                     /locus_tag="PLR47_04615"
     CDS             complement(3419..3901)
                     /locus_tag="PLR47_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOG12257.1"
                     /translation="MAVQFKFFQIPIAHCDEAETALNTFLRSVRALTIHKEFVNQGEN
                     SLWCLAVEYLPQAGEKEFKKGENRNKTDYREILSPEDFALFVKLRDWRKQAATLEAVP
                     VYTILTNEQLAAIAQKRIATLADLKTIDGVGESRAGKYGDAILKIVGMEKNADTGAQA
                     "
     gene            complement(4055..5689)
                     /locus_tag="PLR47_04620"
     CDS             complement(4055..5689)
                     /locus_tag="PLR47_04620"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOG12258.1"
                     /translation="MQVILKKIFVILTMATLLYPSSLTWATERRTALIIGNSQYEVGQ
                     LRNPVNDATDMATTLQGLGFTVILKKNARQQEMEEAIRDFGRRLKRGGVGLFFYAGHG
                     VEINRRNYLLPIGARIERETDVKYQAIDVEMVLDEMANAGNQMNIVILDACRDNPLGR
                     SLRSAGRGLTVISDAPVGTFITYSTSPGKTAADGSGRNSPYTGALLKAISEPGLPIEQ
                     VFKKVRQHLSRVTRNRQIPWELSSLQGDFYFKPGRSGGTNAGANSEKNDLAMGARPPA
                     PEPAQDGILTDTATGMEFVFVKGGCYQMGDTFGGGDGDEKPVHEVCVDDFYMAKHETT
                     VEQFRRFVHDTGYRTEAEKGDGCFVWTGSKWDKSHDANWRSPGFKQTQDHPVACVSWN
                     DATAFVRWLSSKSGKNYRLPTEAEWEYAARSGGKREKWSGTSDGSSLGDYAWYDANSG
                     GQTHPVGQKRPNGLGLHDMSGNVWEWCQDWYGSDYYSSSGRDNPGGPSSGSDRVVRGG
                     GWSSFAAYSRAALRNRRVPDNRYINLGFRLALPPGQ"
     gene            complement(5861..6973)
                     /locus_tag="PLR47_04625"
     CDS             complement(5861..6973)
                     /locus_tag="PLR47_04625"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOG12259.1"
                     /translation="MKRPFILMFLIVFFVPATIFAAELKNERARQEGNRLVISYDLEG
                     KEREADVNLTITVEGKTYKSSDLHVEGDVGKVRTGRGKRISWNILQDFPRGFRGAVDW
                     ELTTGGDAFTDVTTGMSFVFVKGGCYQMGDTFGGGDGDEKPVHEVCVDDFYMAKHETT
                     VEQFRRFVHDTGYRTEAEKGDGCFVWTGSKWDKSHDANWRSPGFKQTQDHPVACVSWN
                     DATAFVRWLSSKSGKNYRLPTEAEWEYAARSGGKREKWSGTSDGSSLGDYAWYDANSG
                     GQTHPVGQKRPNGLGLHDMSGNVWEWCQDWYGSDYYSSSGRDNPGGPSSGSDRVVRGG
                     GWSSFAAYSRAALRNRRVPDNRYINLGFRLALPPGQ"
     gene            complement(7020..7862)
                     /locus_tag="PLR47_04630"
     CDS             complement(7020..7862)
                     /locus_tag="PLR47_04630"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HOG12260.1"
                     /translation="MRENRRFIFFLILTAMLVTPVLVVAGQNPERSGEIKKGIEAEGA
                     CAIVGMSAEQSQLTALQRARAAAIEQAAGVSVTAATLVTNFTLAADFIKTYSRGFVIA
                     EKVTWLPLGQYQKDPSTAPIPEYRVKIVADVYIPKRKAKTLGLAAHLNNAVFRAGEKA
                     KATVKTKSAAGVALFNIMADDTAALLFPNAYEKENHLAPGKTLVFPARDAKVELEMQT
                     LPGHPRDAEAVLVVAWDEASKIDVLKRFTPGEAVPVSEFFARLSEIIDQCEDVILPYE
                     VVAR"
BASE COUNT         1733 a         2486 c         2091 g         1988 t
ORIGIN      
        1 accgccggcg gggttgccgg cttatcgctt gcgacttttg gccatctgcg atgccttatt
       61 ttttcagcac ggcgatgaca tcgtcgggat ttcccatgat caccggctgc tggtccaggc
      121 ccttgagttt ccttgccata tacggaacat tttccttgag aaattctgaa agctcaccca
      181 ccgtgatttt cccattcttg tcctgatccg cctcgccctg cagccccttg agaaaatagt
      241 aggtgaacag ggagtggcgt ttttccggat accaggccga cacctggtcc gcggaggcgc
      301 tcgtaatcac caccgctccc gccggacccc ggtattcttt tttgaccttt accatcgccg
      361 ggctgatgcc cttgaaaagc attcctttat ccgtattgcc ggagaagcag gcatccagca
      421 ccaccgtaat cttctttgcc ggcagcttgg aaaggttgtc atagaaggtc tgaaggcggt
      481 agccgccggc gcggatatac tccggattgc cgtccaccgg gacaaagaag gcgtctccgg
      541 actcgagatc cggcgcaccg tggcccacat agtaaacaaa tacatgggag cggttcggct
      601 tgatgttatt atacagtctt cccttcggct cccgttccgt tccgaaaatt tcattgagct
      661 tggaaagcgt cgtattttcg gcatagatga tgtttccttc gtcaaatccc atggtccgga
      721 caaggtattc cttcatgata cgggcgtcgc gttcggcata ggcgacttcc ggaatgccct
      781 gtccgtaaat cctgttgccg ataacgacgg caacatcata tttgcctgcc ttctgtccca
      841 cgggaatatt ggtgtccaca tccacgctta aaccgccgac aatggcgatg tcttccccct
      901 tttgcgaatc ctgcccggca acctgaacca cctcgatttt cttctgcggc gcattcatca
      961 ccagcgccag ctgactttct ttgttgaagc ggggacgcgc ttccgtcagg gcgacggcga
     1021 ttggtatcgt ttccccgttg gcgatgcggg tgttggtgta aaacatgaac ttgaagtcct
     1081 tgaacctgcc ggaaggcagc gcgcccagtt caaagtgcgt ctgcgcgtcg ccgccgatga
     1141 atacattgct ccccgtcttc acgtctgcaa ccacgccgcg cgcgtcgccc cggccgatgt
     1201 tttgaaccct tacggtcagc tccaccatct ccatcggctc aatgcgtccg ttgccgccgg
     1261 catcgttgat tcctatatcc gccacaacca acttcggctc tgcaaacgct ttggtatgga
     1321 aaaccagctt gacgggcgcc ggatcaaacc cgttggcttc tttgaccgta atggtaaagg
     1381 cggcctggtc cgtcgccatc tcttcctccg ccttcaaatc aatctctttg acgatcgttt
     1441 cacccgcggg aatcgtgccc agaaatactt ccctttcgaa ccttaaaccc ctgatccttt
     1501 ttgccgtatc aacgagggcc ctgcagtcga atgcgtcacc cttgccgctg ttggtgacag
     1561 tgatgacgag ctttcccgtt tcatcggcat ccaggatgtt gtttcccgac ggctcgacaa
     1621 acttgacgga cgtcgtcagc gccggaggaa ccggagagac ggcaatattc acgcggccct
     1681 tccccgcctg cgcggcggag ggaaaaagca gcaaacatcc aaaaacaacg acatatgcta
     1741 ttcttttatg catacacaaa cctccctttc cgggccggct tttaccagtt cccgaatgaa
     1801 gtgattgcgc ttcaaatatc cactccgatt tctttttgat gaacaggcgc atgcacatgg
     1861 gggatgtaat ccgtatcatc ttccatcatc agccgatagg cgtcccggat gttttcttcc
     1921 agttcttcca gcgtttcgcc ctgactgaat acacctggga cttctttcag cctgcccaca
     1981 taccattcgt catccttcca gtattgcagc gtaaactttt tcatctcaaa tctccacccc
     2041 aaacggtaag acacctctca gttcaggcaa cctgcttttg tggccatttc ccctattatt
     2101 tgcggcacgt cctgacacaa gaccggcata ttttccaaat ccccgccgaa gcttttgaac
     2161 cgcccatccg gatggactac cggccgcggc ggggcccgac gctttttgcc cctttgccgg
     2221 ggacaggatt ttgtccgggt cagccggtgg gcatccgctc cgccttctga gctgggggca
     2281 gggcaagccg gaaacccagg ttgttgttgc ggttgtccgg gttgttcctg ttgcgattcg
     2341 ccgcgcgcgt gttggcggca ttgttgttcc aactgccgcc gcgattgacg cggttggaac
     2401 cggaagacgg cccctcagga caaaacccca aaacgggaaa taacattcct tcgaaaacca
     2461 tcgctatcgg caaaacgcgt gaattcaagc aaagaatcca tatgccggac aagttcatcc
     2521 acggaccatt cgccattcat ttgtttgcct tcataaagtc tgaacttatt gacaaatctt
     2581 cttttactgc gtggtgacag cagaatcttt tgcggaaaca ctctgtatcc aagaaaggga
     2641 attcccctgg cacagcggtt catctgaata tccggtttaa ccgtaagcga cagacgcgct
     2701 tgcaggaaat ccttgattgc cggcaattcc acatgcatca atttgtcccg gtcatcatcg
     2761 aaaatcagaa aatcatccat gtaccggaca tagccgcgga cctttcgctc ttccttaatc
     2821 cagtgatcga aaacggacag gtaaaaattg gccaaatgct gcgaaatcag attgccgatg
     2881 ggcaatcctt tgtccgtttc aacttcatag gaggacataa tcttctcgaa aagcaaaaga
     2941 atgtcttttt cacggaagcg acgcgccagc gccccaagca aaatcgcgtg atcaatggaa
     3001 tcaaaatact tccggacatc cagtttgaga aaccaatcga aacgcccggc aaattcctgc
     3061 gctcgcagaa tagctttctg tccgcccttt cctttccggc aggcatagga atcgtaaatg
     3121 gcataggatt ccagaaccgg ctcgcagata ttcatgatcg cgtgatggag aactcgctcg
     3181 ggaaaggaag ccgcgcaaat cagccttttc ttgggatcat aaacctgaaa atagcggtaa
     3241 tgtccgatat caggatttct gtcttccagc tggccgtgaa tcttctcgat gttttcctca
     3301 aagtttttcc tgaaatcgat cacatcgcgg cgcgcctgtt tcccccgggc ggccttgaga
     3361 aaggccagtc ggatattatc ccgatccgct atttgacggt agagatcacc cgcgctcatc
     3421 atgcctgcgc ccctgtgtcg gcgttcttct ccatgccgac gattttaaga atcgcatcgc
     3481 cgtatttacc ggcgcggctt tcacccacgc cgtcgatggt tttgagatcg gcaagcgtgg
     3541 cgattcgctt ttgggcaata gccgccagtt gttcgttggt gagaatggtg tagacgggaa
     3601 ctgcttccag cgtggcggcc tgcttgcgcc aatcgcgcag tttgacgaaa agcgcaaaat
     3661 cttccgggga caggatttca cggtaatcgg tcttgttgcg gttttcgccc ttcttgaatt
     3721 ccttttcgcc cgcctgcggc agatattcga cggccagaca ccacagggaa ttttcaccct
     3781 gattgacgaa ttccttatga atcgtcagcg cccgcaccga tcttagaaac gtgtttaacg
     3841 ccgtttccgc ctcatcgcag tgcgctatgg gaatctgaaa aaacttgaat tgaacggcca
     3901 taataaaaaa tccagtgatt gtttccgctc aacattaata aacagtgcgt gcctttaggc
     3961 tcgctgaatt aatccgtctg cccgcagcat ctctgccgaa tcaggccggc tccgctgcgg
     4021 cctgcttgcg acagagatgc tgaacatgcc cggctcactg acctgggggc agggcaagcc
     4081 ggaaacccag gttgatgtag cggttgtccg ggacgcgcct gttgcgaagc gccgcgcgcg
     4141 agtaggcggc aaagctgctc caaccgccgc cgcgaacgac gcggtcggaa ccggaagacg
     4201 gccccccagg attatccctg ccgctgctgc tgtaataatc actgccatac caatcctgac
     4261 accattccca gacattgccg ctcatatcgt gcagtcccag accattcggc cgcttctgcc
     4321 ccacggggtg cgtctgtcca ccggaattgg catcatacca ggcataatcg cccaaagatg
     4381 atccatcgct cgtcccggac cacttctccc tcttgccgcc gcttcttgcc gcatactccc
     4441 actcggcctc cgtgggcagg cgataatttt ttccggattt gctcgatagc catctaacga
     4501 aagccgtcgc gtcattccag gagacacagg caacaggatg atcctgtgtc tgtttgaaac
     4561 cgggactgcg ccagttggcg tcgtgagact tatcccattt agaaccagtc caaacaaagc
     4621 agccatcacc tttttccgct tctgtccgat agcccgtgtc gtgaacgaac cggcgaaact
     4681 gttcgaccgt cgtttcatgc tttgccatgt agaaatcatc cacgcagact tcatgaaccg
     4741 gcttttcgtc cccatcacca cccccgaagg tgtcgcccat ctgataacag ccgcctttga
     4801 cgaagacaaa ctccatcccc gtggcagtat cggtaaggat cccgtcttgt gccggttccg
     4861 gcgccggggg tctcgctccc atggcaaggt cgtttttttc gctgtttgcg ccggcgttcg
     4921 taccaccgga ccgtccgggc ttgaagtaga aatccccttg cagggacgac aattcccacg
     4981 gaatctgccg gttcctcgtt acccggctca aatgctggcg tactttcttg aatacctgct
     5041 cgatgggcag ccccggttca ctgatggcct tgagcaatgc acccgtgtaa ggactgttgc
     5101 ggccgctccc atcggctgcc gtcttcccgg gcgatgtgga ataggtgatg aacgtcccca
     5161 ccggggcatc ggagataacc gtaagccccc ttcccgcgct gcgcagggat cgccccaatg
     5221 ggttgtcccg gcaggcatca aggatcacga tattcatctg gttgcccgca ttggccattt
     5281 cgtccagaac catctccaca tctatcgcct gatacttgac gtctgtttct ctctcgatcc
     5341 gcgctccgat agggagcaag tagttccgac gattgatctc gacgccatgg ccggcataaa
     5401 aaaagagccc aacgccgccg cgtttgaggc gccggccaaa atcacgaatt gcttcttcca
     5461 tctcctgctg ccgggcattc ttcttcaaaa taaccgtgaa accaaggccc tgaagtgtag
     5521 tcgccatatc ggtggcgtcg tttacgggat ttctcagttg ccccacctca tattggctgt
     5581 tgccgatgat cagtgccgta cgccgttccg tcgcccacgt caatgaagaa ggatataaca
     5641 aagtggccat tgtcagtatc acgaagattt tcttcagtat gacttgcatg gaattttcat
     5701 cctaacctaa gaatatatgg tttacatact tcaggatacg aaaataaaca gtgcgtgcct
     5761 ttaggctcgc tgaattaagc cgtctgcccg cagcatctct gccgaatcag gccggctccg
     5821 ctgcggcctg cttgcgacag agatgctgaa catgcccggc tcactgacct gggggcaggg
     5881 caagccggaa acccaggttg atgtagcggt tgtccgggac gcgcctgttg cgaagcgccg
     5941 cgcgcgagta ggcggcaaag ctgctccaac cgccgccgcg aacgacgcgg tcggaaccgg
     6001 aagacggccc cccaggatta tccctgccgc tgctgctgta ataatcactg ccataccaat
     6061 cctgacacca ttcccagaca ttgccgctca tatcgtgcag tcccagacca ttcggccgct
     6121 tctgccccac ggggtgcgtc tgtccaccgg aattggcatc ataccaggca taatcgccca
     6181 aagatgatcc atcgctcgtc ccggaccact tctccctctt gccgccgctt cttgccgcat
     6241 actcccactc ggcctccgtg ggcaggcgat aattttttcc ggatttgctc gatagccatc
     6301 taacgaaagc cgtcgcgtca ttccaggaga cacaggcaac aggatgatcc tgtgtctgtt
     6361 tgaaaccggg actgcgccag ttggcgtcgt gagacttatc ccatttagaa ccagtccaaa
     6421 caaagcagcc atcacctttt tccgcttctg tccgatagcc cgtgtcgtga acgaaccggc
     6481 gaaactgttc gaccgtcgtt tcatgctttg ccatgtagaa atcatccacg cagacttcat
     6541 gaaccggctt ttcgtcccca tcaccacccc cgaaggtgtc gcccatctga taacagccgc
     6601 ctttgacgaa gacaaacgac atgccggtcg taacatcggt aaaagcgtcc ccgcccgtcg
     6661 tcagttccca atccaccgcg ccgcgaaaac cgcgagggaa atcctgcagg atgttccagc
     6721 ttatgcgttt gccgcgcccc gtccggacct tgcccacgtc gccctcgacg tgcaggtcgg
     6781 aggatttgta ggttttgcct tccacggtaa tggtcaggtt gacgtccgcc tcccgctcct
     6841 tcccctccag atcgtagctg atcaccaggc ggttcccctc ctgccgcgcc cgctcgtttt
     6901 tgagttccgc cgcaaaaatg gtggcgggaa cgaaaaagac aatcaaaaac attagaataa
     6961 acggtcgttt catcggtttc ctcattcttt tccgggcctg aaagcccgga ccgcgttttc
     7021 tatcgcgcga cgacttcata gggcagaatg acatcttcac actggtcaat gatttccgag
     7081 agccttgcga aaaattcgct cacggggacg gcctcacccg gcgtaaagcg tttcaggaca
     7141 tcgattttcg acgcttcatc ccatgcgaca accaaaacag cctcggcatc tcttgggtgg
     7201 ccgggcagcg tctgcatctc cagctccact ttggcgtctc ttgcgggaaa gacaagggtc
     7261 ttccccgggg caagatggtt ttccttctcg taggcattgg gaaacaggag cgccgcggtg
     7321 tcatcggcca tgatgttgaa cagcgcgact ccggccgcgc tttttgtttt gaccgttgct
     7381 tttgccttct cgccggcgcg gaaaacggca ttgttgagat gggcggcgag ccccagggtt
     7441 tttgctttcc gtttgggaat atagacgtcc gcgacgattt tgacccgata ttccgggatg
     7501 ggcgctgtgg atgggtcctt ctgatactgc cccaggggca gccaggtgac tttctcggcg
     7561 atgacaaagc cgcgtgaata cgttttgatg aaatcggcgg caagggtgaa attggtcacc
     7621 agcgtggcgg cggttacgga aacacccgcc gcctgctcga tggccgccgc ccttgcccgc
     7681 tgcagggccg tcagttgcga ctgctcggcg ctcatgccga cgatggcgca ggcgccttcg
     7741 gcctcaatgc cttttttgat ctcgccgctg cgttcggggt tttgcccggc gacgaccaaa
     7801 acgggcgtga ccagcatcgc cgtcaggatc agaaaaaata tgaagcgcct gttttccctc
     7861 attttcaacc tcaatatttt caacctccat ccgtcttgtg aaccctgcat agctttgacg
     7921 gacgtaaaaa agcgtcgccg ccgtcaccct gagcccgtcg aagggtgaac gtaaatattc
     7981 gatatcacat catgcttcga cgggttcccc atgacggagg gacgatttcc gctcttggct
     8041 gcctgacgca ctgccgcatc ccgtcgatca tttccacggt cgcgaggtta tggatgaact
     8101 tttccgtttg ataaggcatt tccctgacgg ttttgagtat agggtggccg ttttctcaag
     8161 tcaagagaga tttccccggg gtgtcgcgag cacacgctct gtgaggagga cgaggacggc
     8221 cttcggcggt atggtcacgc cagggatcac cggccggatc tcccgcagat cgttatcggt
     8281 ctggcggtga cgaaggag
//
LOCUS       DAOSIM010000072         8246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_8097_length_8246_cov_8.757050, whole genome shotgun sequence.
ACCESSION   DAOSIM010000072 DAOSIM010000000
VERSION     DAOSIM010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8246
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8097_length_8246_cov_8.757050"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PLR47_04635"
     CDS             <1..512
                     /locus_tag="PLR47_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HOG12261.1"
                     /translation="GLTGFGASAHLVLQLARFLYPHSKISVFARSEHERRFALALGAG
                     WVGDTSEKSPEKLAAVIDTTPAWKPVACALENLAPGGRLLINAIRKEEADKDQLLKLD
                     YPLHLWQEKEIKSVANVARRDIAEFLDLAAKIPILPDVEEYAFFDANRALAELKAGQI
                     RGAKVLKIE"
     gene            complement(582..911)
                     /locus_tag="PLR47_04640"
     CDS             complement(582..911)
                     /locus_tag="PLR47_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12262.1"
                     /translation="MQENFGHKMRGAPLMTALKSHCDIHPSEKSVHRLVGSPAKLAFA
                     GIQTNFTVTHFYVMRYDVMTECNHPRNIRDPMEVIVIHFGLKGWTPMKIAGCLTSAIR
                     KNRMNLK"
     gene            complement(992..2431)
                     /locus_tag="PLR47_04645"
     CDS             complement(992..2431)
                     /locus_tag="PLR47_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOG12263.1"
                     /translation="MVGAGIAGLSAAWELEQKNIDVAVIEMAPTYGGTGLTSEGALCI
                     IGTPEQEQGGDPDDPRMAYADFMTAGRDEQGPGPNAEWVNYYVKESRREIYDWLTGFG
                     VRFEKKVILMPDNTVPRWHKVVGKGRGLLEPIYRDCGKNGKVKFYYRFKAVSLLRDKS
                     GRIEGVRTLRLKDVAQIDFFAPVVILATGGFQSNLDMVRKNWPKDLPFPDEILIGGGL
                     NADGSGHAMAKTVGARLDNMHYQLNYPTGLKRPSNATGHRGLNAYTDHSIWVNKTGKR
                     FMNESRDTRKTFPEVVRQPGGTYWAIFDNEARKVFHVSGWSRQLVESELFNNPQYSEV
                     VKSAMTIRELGVAAGLSPETLEETVRRWNEMVTAGRDKDFGRIGTSRSVWAHPPRIEK
                     PPFYALRFMPLTRKSMGGVAIDQSCRVINVSGQTIPGLYAVGELTGLAGVNGKCTLEG
                     TFLGASILTGRVAGRAAAAELAKRKETPH"
     gene            complement(3262..4110)
                     /locus_tag="PLR47_04650"
     CDS             complement(3262..4110)
                     /locus_tag="PLR47_04650"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG12264.1"
                     /translation="MTPVSAIIRRRFSCRSYDNKPLDDDLLRTFRQAVEASAPGLFGN
                     APRFVLLEMDELPRDVWKKLGTYGVIQNARLYLAGMIRPAPMATVDYGYCKEKLILKA
                     TELGLGTCWLGGTFAISAFGRAAGMRRDELMPTVSPVGFATGRLRLAERILRGFAGSD
                     HRLPWTELFFRDSTAAPLSAGEAGPYAEALENVRLAPSASNKQPWRIIREPEQSAFSF
                     YLSRSAGYRHLRDVSLQEIDMGIAMCHFDLTVKASGIAGSWHQDDAAPKIKSWEYTAA
                     WQAVGE"
     gene            4214..5785
                     /locus_tag="PLR47_04655"
     CDS             4214..5785
                     /locus_tag="PLR47_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOG12265.1"
                     /translation="MKLATVEQMREMDGYAVEKLGMAEEILMENAAHAAVSLLQKTFG
                     IQGRMFVIFCGAGNNGGDGLALARLIKSAGGRVKVFLTASPKKYGGAAKINYDILCNL
                     DADIQLLTKAQESRADVLAADVLVDAIFGTGLDRDVRGLAADVIALVNASGRPVVSLD
                     IPSGISGNSGQVMGEAIRATHTVAFGLPKLGNLLYPGYEHCGALWVTHISFPPSLYES
                     DALTVETNGTATALPPRPAQAYKSSMGDVLFIAGGANYFGAPYFSAMAFLKSGGGYAR
                     LAAPASIIPFVAQSGREIVYLPQRETALYSLSLKAKPDLLALAEKADMVVMGPGLSLQ
                     EETTKLIRELAAVISRPLLIDGDGLTAIAERPEILLKRKAETILTPHAGEMARLTRKT
                     IACVNADKIGVLREASKKLNATVVFKGPHSLIGLTDGRVYVNLSGNPGMATAGSGDVL
                     TGCIAAMHGLGLPPPLATRKGVFLHGCAGDLAAAQKGRDGMTATDILEHLPQALKNDR
                     ESTAAEKQYEPPVLF"
     gene            5903..6313
                     /locus_tag="PLR47_04660"
     CDS             5903..6313
                     /locus_tag="PLR47_04660"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOG12266.1"
                     /translation="MAGIPHLQWSNSISVRVDSIDAQHKKLFEITNRLIDAFEAGSGD
                     FLEVIGELVEYTTIHFHDEQMVMMNAKFPGLAAHTAEHDKFIAKVEDFLQAYSEGHKD
                     AGRTMLVFLKDWLFAHTSGLDMEFAQYLAKKSAK"
     gene            6351..>8246
                     /locus_tag="PLR47_04665"
     CDS             6351..>8246
                     /locus_tag="PLR47_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOG12267.1"
                     /translation="MADASKIAELQDTLRQQKGLQNLTNRINAATGIPQMVAEVRPLL
                     IDLFRVEAAHVYVREKGRKDVHTFIAPAGQTAEVKTALGPQTLPGYVVNTGKMIHVND
                     VGDPGQTEAYQDFPLGEGENRRFGVGVRQILAMPVFYGGETIGAVEVINKKEAAPFGQ
                     KEPALLQNISGLLGVALYNFLLIDPKAKRLKGTYLVSNGLITQEAMDRAKEEARVTKE
                     PLQDVLVKKFNISKEDVGRACEFFWQCKFVSFDKKTPIPGELLKNLKRDYLIREVWVP
                     LEKTEKGVRVIMDDPQDILRRDAIQKSLKIKSVQFDVALKEDILQFIDLFMQSGAEEA
                     SFSDLLGKLETGEGEKKEEPEDVDESDSAIIQLVNKIIGDAFNRRASDIHIEPNVIDK
                     NVLVRFRVDGDCALYQTVPYSYRSALISRIKIMANLDITVKRVPQDGKIKIKRSGADE
                     IELRVVTIPTQGGVEDVVMRILAKGDALPLSAMRMSQKNYQDILKICEQPYGMILVVG
                     PTGSGKTTTLHAILNHINKPDRKIWTAEDPVEITQRGLRQVQVQPKIGFDFAAAMRAF
                     LRADPDVIMVGEMRDFETAKIGVEASLTGHLVLSTLHTNSAPETISRLLDMGIDPLNF
                     ADSLLAVL"
BASE COUNT         1874 a         2273 c         2339 g         1760 t
ORIGIN      
        1 tggggctcac gggattcggc gcttcggccc acctggttct gcagcttgcc cgctttctct
       61 atccccattc caaaatcagt gtctttgccc gctccgaaca cgaacgtcgt tttgccctgg
      121 cgctgggcgc gggctgggtg ggggacacct ctgaaaaaag tccggagaag cttgccgccg
      181 tcatcgacac cacgcccgcc tggaagccgg tggcctgcgc gctggaaaat cttgcgccgg
      241 gtggaaggct gctgatcaat gccattcgga aggaggaggc ggacaaggac cagttgctca
      301 aacttgacta cccgctgcat ctctggcagg agaaggaaat caaaagcgtg gccaacgtgg
      361 ctcgccggga tatcgcggaa ttcctcgatc ttgccgcgaa aataccgatc ctccccgacg
      421 tggaggaata tgcttttttc gacgccaacc gggcgcttgc ggaactaaaa gccggccaaa
      481 tccgcggcgc gaaggtcctg aaaatagagt agcgacgatc tatgagcaac gagtactaga
      541 accttattta aaagggttct tcaggttttg ttatgcagaa attattttag gttcatccgg
      601 ttcttgcgta tagccgaagt caagcaccca gcaattttca tgggcgtcca acctttcaat
      661 ccgaaatgaa tgacgatcac ttccatggga tcccgaatat tcctgggatg attacattca
      721 gtcatgacgt cataacgcat cacataaaaa tgcgtgacgg tgaagtttgt ctgaattccg
      781 gcgaatgcca gttttgcggg tgaaccaacc aaacggtgaa cggatttctc agaaggatgg
      841 atgtcgcaat gtgacttcaa cgcggtcatt agcggcgcac cccgcatctt gtggccaaaa
      901 ttttcttgca cacacaagac ggcatttttt atacttcacc gcacaaaaag gaaattctta
      961 tcaataattc aattcaataa taattatcaa ttcaatgcgg cgtttccttc cgctttgcca
     1021 attccgccgc tgccgcgcgt cccgcgacgc ggccggtcag gatggacgct cccaggaaag
     1081 tcccttccag tgtgcatttt ccgttgacgc cggcaagacc cgtcaattca cccacggcgt
     1141 acaatccggg gatggtctga cccgagacat tgatgacacg gcaggactga tcaatggcga
     1201 cgccgcccat gcttttgcgc gtcagcggca tgaagcgcag ggcgtaaaat ggaggctttt
     1261 cgattctggg gggatgcgcc cagactgatc gtgacgtgcc gatgcgtccg aagtccttgt
     1321 cgcggcctgc cgtcaccatc tcgttccagc ggcgcacggt ctcctccagg gtctcgggag
     1381 aaagtcccgc cgccacgcca agctcgcgga tggtcatggc cgacttgacg acctcgctgt
     1441 actgtggatt gttaaagagt tcgctctcca ccagttgacg gctccaaccg gagacatgga
     1501 ataccttgcg ggcttcatta tcaaatatcg cccagtaggt tccgccgggc tgccgcacga
     1561 cttccggaaa tgtcttgcgt gtgtcgcggg actcgttcat aaaacgcttt cccgttttgt
     1621 tgacccaaat ggaatgatcg gtgtaggcat tcaatccgcg atggccggtc gcgttggatg
     1681 gtcgtttcag accggtcggg taattcagct gataatgcat attgtccagg cgggctccga
     1741 cggtctttgc cattgcgtgt ccggaaccat cagcgttcag gcctcctccg atgaggatct
     1801 catcgggaaa gggcagatcc ttcggccagt ttttgcggac catgtccaaa ttgctctgga
     1861 atcctcccgt ggcaagaatc acgaccggcg cgaaaaaatc gatctgtgcg acgtctttga
     1921 gccttagcgt gcggacgccc tcgatgcggc cgcttttgtc ccggagcagg gaaaccgctt
     1981 taaaccggta ataaaatttc actttgccgt ttttgccgca gtcgcggtag atcggttcaa
     2041 gaagccctct gcccttgccg acgaccttgt gccagcgcgg cacggtattg tcgggcatca
     2101 gaatcacttt cttttcaaaa cggacgccaa agcctgtcag ccagtcgtaa atttcgcggc
     2161 gggactcttt cacatagtag ttgacccatt cggcattggg accgggaccc tgctcatcgc
     2221 ggccggccgt catgaaatcc gcataggcca tgcggggatc atccggatca ccgccctgtt
     2281 cctgctcggg agtgccgata atgcacagag cgccttcact cgtcagcccg gttccgccgt
     2341 aagtgggcgc catctcaatg acggcaacat cgatgttttt ttgctccagt tcccacgcag
     2401 ctgaaagccc cgcgataccc gcgccgacca caattacacc atgccgttgt tctttttgag
     2461 ccgccatgac cacagccgat aaacataaga ccatagccat ggaaaggaac agtacttttt
     2521 tgaatagctt catctcaacc tctcaaaaat gcgttatttc agacattgct ttccgcaatc
     2581 gatccaaaat tgatttcagc aattgaaaag ggtgtgcaga ttcgggtaaa agtgtcatcg
     2641 gaataagcgg atataaatcc gaagagcagg cgccccgaaa aacgcgtgta acaaaatatc
     2701 gggtcaaatt ccacactttt ttcccggatg ggaatttttt gaggttgctg tcgattcagg
     2761 gaatggcggg tgttgaagaa ctaaggggaa gaactaaggg tcgaatcttt actcttgacg
     2821 tttcagattg ataacgtggt aatctgtcac cattgcgatc tttccggcgg ctggagaatg
     2881 ttcttcaacc gctttttgtt tgcttggaac ggtttgtctg gatgttttat gcggctgtat
     2941 taccccaccc atagaatgaa gcatatttgc tgctttgaag tggcaatcat cactccaatc
     3001 ccaaattacg aattgcaact tgcaggcatc aggtgaaaca cagattcact cctcccattt
     3061 taccccgacg tcccagggta tcttatgcca attgataatt tttatgaaca caaggtgaaa
     3121 gataagtacg cagcaaccgg atgaacctta aaacctttat ggaggcaaaa gaagatgatc
     3181 actcaaagaa gcttcttgag gccattatgg gtgtagaata attggcaata acaaaccggg
     3241 aatttcaaaa tgaatatagg tctattcccc cactgcctgc caggcggccg tgtattccca
     3301 tgatttgatc tttggcgcgg catcgtcctg atgccagctt cccgcgattc ccgatgcttt
     3361 gacggtcaga tcgaagtggc acatggcgat gcccatgtcg atttcctgca gtgaaacatc
     3421 cctcaggtga cggtatcccg cactgcgcga cagatagaat gaaaaagcgc tttgctccgg
     3481 ttcgcggatg atccgccagg gctgtttgtt ggaggccgaa ggcgccagac ggacgttttc
     3541 cagggcttca gcgtaagggc ccgcctcccc ggcggaaagc ggggcggcgg tgctgtcgcg
     3601 aaagaaaagt tccgtccatg gcaggcgatg gtcggagccc gcgaagccac gcaggatccg
     3661 ctccgccagc ctcagtcgcc ccgtcgcgaa gcccacggga ctgacggtcg gcatcagttc
     3721 atcgcgccgc atgcccgccg ctcggccgaa ggcgctgatc gcgaaggtgc cgcccagcca
     3781 gcaggtgccc aggcccagtt cggtggcctt caggatgagt ttttccttgc agtatccata
     3841 gtcaaccgta gccatcggcg cgggtcggat cattcccgcg aggtacagcc gggcattttg
     3901 aatcacgccg tacgtgccca gttttttcca gacgtcacgc ggcagttcgt ccatctcgag
     3961 caggacgaag cgcggcgcgt tcccgaacaa tcccggcgcg gacgcttcga cggcctgccg
     4021 gaacgtccga agaaggtcat cgtccagagg tttattgtcg taagatcggc aggaaaaacg
     4081 cctgcggatg atcgcggata ctggggtcat atcgcctccc gaaaatattt attgtcaaat
     4141 attgcgcgat tcctttatac tatcaacaaa aaaattgatt cacgactttt ccatttggag
     4201 gaatagcggg actatgaaac tcgccacggt tgaacaaatg cgtgaaatgg acggctatgc
     4261 cgtcgaaaag ctggggatgg cggaagaaat actcatggaa aacgcagcgc acgcggctgt
     4321 atccctgctg caaaagactt ttggcatcca gggcaggatg tttgtcatct tctgcggcgc
     4381 cggcaacaac gggggcgacg gtctggctct ggcccggctg attaaatcgg ccggcggccg
     4441 ggtaaaagta tttttgacgg cttctcccaa aaaatacggc ggggccgcca aaatcaacta
     4501 tgacatttta tgcaacctgg acgcggacat tcagcttctg acaaaggccc aggagtcgcg
     4561 cgccgacgtg ctggccgccg acgtcttggt ggatgcgatt ttcggcacgg ggctggaccg
     4621 cgacgtcagg gggctggcgg ccgatgtgat tgcgctcgtc aacgcctccg gccggccggt
     4681 ggtgagtctg gacattccct ccggcatctc cggcaacagc gggcaggtga tgggtgaggc
     4741 aattcgcgcg acgcacacgg tggcgttcgg tttgccgaag ctcgggaacc ttttgtaccc
     4801 cgggtatgaa cattgcggag cgctttgggt gacgcacatt tccttcccgc cttcgctcta
     4861 tgagagcgac gcgctgacgg tggaaaccaa cggtacggcg acggctctcc ctccgcgtcc
     4921 cgcccaggcc tataaaagct ccatgggcga cgttctgttc atcgccggag gggccaacta
     4981 cttcggcgcg ccgtattttt ccgcgatggc ttttctgaaa tcgggcggag gctacgcgag
     5041 gctcgcggcg cccgcttcaa tcattccctt tgtggcccag tccggccgcg aaatcgttta
     5101 cctgccgcag cgcgaaacgg cgctgtacag cctctcgctc aaagccaaac cggacctgct
     5161 ggcgcttgcg gaaaaagccg acatggtggt catgggcccc ggcctttcgc tgcaggagga
     5221 gacgaccaag ctcatccggg aactcgcggc cgtcatctcc cgtccgcttt tgatcgacgg
     5281 ggacggactg acggccatcg ccgaacggcc ggaaattctg ctcaaacgga aggcggaaac
     5341 gattctgacg ccgcacgcgg gcgagatggc gcggctgacg cgaaaaacca tcgcctgcgt
     5401 gaatgccgac aagatcggtg tcctgaggga ggcgtcgaaa aaactgaacg ccacggtggt
     5461 tttcaaaggc ccgcattccc tcatcggcct gacggacggc cgggtgtatg tcaatttgag
     5521 cggcaatccg ggcatggcca ccgccggaag cggcgacgtc ctgaccggct gcatcgcggc
     5581 catgcacggc ctggggctgc cgcccccgct tgccacccgc aagggggttt tcctgcacgg
     5641 ctgcgccgga gatctggccg ccgcccaaaa aggccgggac ggcatgacgg cgacggatat
     5701 tctggagcac ctgccgcagg cgctgaaaaa cgaccgcgaa agcacggcgg ccgaaaagca
     5761 atacgagccg cccgtcctgt tctagagaga ggcggctccc ggacggtatg gcttgcttca
     5821 gggggctcac ttcgggattg actttccccc ggagccgcgc ctatactccc gttaatacaa
     5881 tacgaaagac gggagaaatg acatggccgg aataccccat ctccagtggt ccaacagcat
     5941 cagcgttcgc gtggacagca tcgacgccca gcacaaaaaa ctttttgaaa tcaccaatcg
     6001 cctgatcgat gcttttgaag ccggatccgg cgattttttg gaggtgatcg gcgaactggt
     6061 ggaatacacg accattcact ttcacgacga gcagatggtg atgatgaatg cgaagtttcc
     6121 gggcctcgcg gcgcataccg cggagcatga caaattcatc gcgaaagtcg aagattttct
     6181 tcaggcttac agcgaaggtc acaaggacgc ggggcggacg atgctcgttt ttttgaaaga
     6241 ctggcttttc gcccacacct cgggcctgga catggaattt gctcagtatt tggcaaaaaa
     6301 atccgcgaag tgaaatccga tcgcctcaaa cgaacaagaa aggtttgacc atggcagacg
     6361 cgtccaaaat tgccgaattg caggatactt tgaggcagca gaagggattg cagaacctca
     6421 ccaaccgcat caatgccgcg accggcattc cacagatggt tgccgaggtt cgtccgctgc
     6481 tgatcgatct gttccgggtt gaggccgcgc atgtgtatgt tcgggaaaaa ggccgcaaag
     6541 acgtacatac ctttatagcc cccgccggtc agaccgctga agtgaaaacg gctctcggcc
     6601 cccagaccct tcccggatac gtcgtgaata cggggaaaat gattcatgtc aacgacgttg
     6661 gcgatcccgg ccagaccgag gcctaccagg actttccgct cggcgaaggc gaaaaccgcc
     6721 gtttcggcgt cggcgtgcgg cagatcctgg ccatgccggt tttttacggc ggcgaaacga
     6781 ttggcgcggt cgaggtgatc aacaaaaagg aagccgcgcc gttcgggcaa aaagagccgg
     6841 cgcttttgca gaatatttcc ggactgctcg gcgtggccct ctacaatttt ctgctgatcg
     6901 atcccaaggc aaaaagattg aaaggcacct atctggtttc caacggcctg atcacgcagg
     6961 aagccatgga ccgggccaag gaagaagccc gggtgaccaa ggagccgctc caggacgtgc
     7021 tggtgaagaa attcaacatc agcaaagaag acgtcggacg ggcctgcgaa tttttctggc
     7081 agtgcaaatt tgtttctttc gacaagaaaa cgccgattcc cggcgaactg ctgaaaaatc
     7141 tgaaaagaga ctacctgatc cgggaagtct gggtccccct ggaaaaaacg gaaaaaggcg
     7201 ttcgcgtgat tatggatgat ccgcaggaca ttctccggcg cgacgccatt caaaagtccc
     7261 tcaagataaa atccgtgcag tttgacgtgg cgctgaaaga ggatattctc cagttcattg
     7321 acctgttcat gcagagcggc gcggaagaag cgtccttttc ggatcttttg ggaaagcttg
     7381 agacggggga aggcgagaag aaagaagagc cggaagatgt ggacgagtcc gacagcgcca
     7441 tcattcaact ggtcaacaag atcatcggcg acgcgttcaa ccgccgggcg tcggacattc
     7501 atatcgaacc gaatgtgatc gacaagaacg tgctggtgcg gttccgggtg gacggggact
     7561 gcgcgctgta tcagaccgtc ccttactcct atcggtcggc gctgatttcc cgcattaaaa
     7621 tcatggccaa tctggacatc accgtgaagc gggttccgca ggacggaaag atcaagatca
     7681 agcggtccgg cgcggatgaa atcgagctgc gcgtcgtgac gattccgacg cagggcggcg
     7741 tggaggatgt ggtgatgcgc atcctggcca aaggcgacgc cctgccgctt tccgccatgc
     7801 ggatgtcgca aaagaactat caggatattc tgaaaatttg cgaacagccc tacgggatga
     7861 ttctggtggt cggaccgacc ggttcgggaa aaacgaccac gctgcacgcg attttgaacc
     7921 acatcaacaa gccggaccgg aaaatctgga ccgccgaaga tcccgtggaa atcacccagc
     7981 gggggttgcg gcaggtgcag gtgcagccga aaatcggatt tgatttcgcc gcggcgatgc
     8041 gggcgtttct ccgcgccgat ccggacgtga tcatggtcgg tgaaatgagg gattttgaaa
     8101 cggccaagat cggcgtcgag gcgtcgctta ccgggcatct ggtgttgagc accctgcata
     8161 ccaacagcgc tccggagacg atttcccggc tgctggacat ggggatcgac ccgctcaatt
     8221 tcgcggattc cctgctggcg gtgctg
//
LOCUS       DAOSIM010000073         8233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_8107_length_8233_cov_6.373319, whole genome shotgun sequence.
ACCESSION   DAOSIM010000073 DAOSIM010000000
VERSION     DAOSIM010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8233
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8107_length_8233_cov_6.373319"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLR47_04670"
     CDS             complement(<1..290)
                     /locus_tag="PLR47_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG12268.1"
                     /translation="MNIKDFRQQENGVHEIFLHRWSPRAMSGADLDEAALLNLFEAAR
                     WAPSANNNQPWRFIYARRDTPRWEVFFELLAEGNRIWAKNAAVLIAVISKTT"
     gene            458..533
                     /locus_tag="PLR47_04675"
     tRNA            458..533
                     /locus_tag="PLR47_04675"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:492..494,aa:Glu,seq:ttc)
     gene            complement(550..1557)
                     /locus_tag="PLR47_04680"
     CDS             complement(550..1557)
                     /locus_tag="PLR47_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOG12269.1"
                     /translation="MSRPLRIQYPDAFYHVTCRGNERREIFRHADDRKEFFRLLSRSL
                     TIFEVQLLAYAMMPNHFHLLVCTPKGNLSEFMRHFNISYTGFFNRKHKRTGHLYQGRY
                     KAFLIDENNYLMEVSRYIHLNPLRIKSQKPVGQRFRDLMINDSTSFPGYLQPQNRKSF
                     VQYKIILDYFGGENHNAMNEYQKFVVEGMERDLPSPLEKGKGTGIVGGEDFIEQIQKT
                     FSKSKGHQRSSREQPALRKLGKQIKPDHLIDRYARLVKISREDLTRKGKQSTQRAMLM
                     EILYRICNLTQPEIGSLLGGVDYSAVSQARKRLQIKIENNPELGGELIKIQNELVKCQ
                     E"
     gene            complement(1662..2747)
                     /locus_tag="PLR47_04685"
     CDS             complement(1662..2747)
                     /locus_tag="PLR47_04685"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006941.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase [NAD+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HOG12270.1"
                     /translation="MFKKAVFPGKYIQGAGALKELPALISLFGARALVLASPTAAKTI
                     VPVCGIHEETNQAFIETFGRECCEKELVRLAKVIEKNRIGVMIGMGGGKTIDTAKIAA
                     DRANIPVIIVPTIASTDAPCSGCAVVYSENGVHEREYYQKSNPAAVVVDTGIIAAAPV
                     RFLVAGMGDALATWFEAESCRRTQSKNECGGLSTMAGLHLARLCYDTLLACGAAAKLA
                     CEHQVPTPALERIVEANILLSGIGFESSGLAAAHAIHNGLTALEETNAYYHGEKVAFG
                     VLTGLHLTGASPEVMRTVYGFCREMGLPVTLADIGLDKRDRIGLRKAAEKTCAPGAAV
                     HHEAGDITPERVLHAMIAADAAGQKMR"
     gene            2997..3668
                     /locus_tag="PLR47_04690"
     CDS             2997..3668
                     /locus_tag="PLR47_04690"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOG12271.1"
                     /translation="MHGADNARFWTDQLFNAMITADRREASGVMDRMMAAGVNSQSII
                     DDVLDPTLIRLGDLWEQDAVSLTQTFVASKIAEDVLLRCVSASGKTAKRGAPVVIGNI
                     EDDFHSLGRRIVGSFLRAGGWEVHDLGNDVTAEQFVDKAIEVGASVVGASAMMHTTAL
                     NIRKLRDLIDARGLNHQLKLAVGGAVFNWRPDLVAEVGGDGTARNATGVDALFQRLRD
                     AAKGG"
     gene            3665..4567
                     /locus_tag="PLR47_04695"
     CDS             3665..4567
                     /locus_tag="PLR47_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOG12272.1"
                     /translation="MSTINSCERVMNTLQGLPTDRVPVFAVLGAYGGKLAETGLKTLY
                     SDADAYVSGQQAVQNALGFDMILATFDYSAIAEAFGGEVAWFDNQTPNMKRPAVSEAS
                     AVAGLKLPDPRRDARLPVILSATRQLVSLYRESVPVFAAVPGPGALPALVLGLETWME
                     TVLFDEDNARRILERTGRFFLDWVHALCEAGVTGLVVTESVASVEISTRDLFAKVFLP
                     HLHDLFGQINAPLVFHHGGGRILHILDLLPGLPGLAGVVVGSKDDLGAARSALGTELT
                     LIGNLDNLSLPAATFCRTPPRMCR"
     gene            4641..7208
                     /locus_tag="PLR47_04700"
     CDS             4641..7208
                     /locus_tag="PLR47_04700"
                     /inference="COORDINATES: protein motif:HMM:NF019413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG12273.1"
                     /translation="MNPSTIWLSCGVLQAELEQLHRDGRIRGELRFLDSMLHMNPERL
                     QAALEAEVAKLVSEGSRVVLVYGDCCNRMTDLIRRFGVSRVNAVNCAQMLAGRRRYRE
                     LMKNQAFMLLPEWAPRWKEIMQGELGLSPEVARDLMRENRRELVYLDTAVAPAPREAI
                     EECASYTGLPLRVEKVSLDHLLALLLQAESGRIPPLPEKAVASSAPYRKPATWADQVM
                     MADVFSEILASSDPSRLAQILTEQMRELTGAHTAILVAHPEGVHSHRIMHVSPARRGS
                     LLTADEVRLFCSDLAQVMPLYVKDYHPEHPCRLPLLRAGVSSLMRFPLRAGEDLLATM
                     LLLDLPGADRIAETEKIITHLTPVMALALKNSFAREEIERQAKKLAYYAGDLERRVAQ
                     RTEALRESEEKYRTLFENAGEAIFVAQDGRVVFLNPMMTQITGYSGEELADRPFTDFI
                     HAEDRSKIFDRHTRRLHGENIPRSYAFRAFHKNGFLIWVRLDTILIDWQGKPATLNFM
                     ADITKRKQAEEALQDAYNRLNKIIDFLPDATFVVDRSGTVIAWNRAMEQMCGISKAEM
                     MGHGNHEYSLPFYGQRRPLLIDLALLPDEVFEKSHYENIARQSDNLQAESYVPGVYGG
                     KSAFLWGIASRLRDVSGNIIGAIESIRDITDRKNSMDQLRKALGGTVQAIALVVESKD
                     PYTAGHQRRVADISRAIATEMGLSQDQIEGIRMAGIIHDIGKVSVPAEILSMPRKLTH
                     LEFSLIQTHAQSGYDILKDIEFPWPIARMVLEHHERMNGSGYPNGLTGDKLLLESRIL
                     AVADVVEAMATYRPYRAALGLSAALEEIAQNRGILYDPAAVDACLHLFREKGFTIKE"
     gene            complement(7227..7907)
                     /locus_tag="PLR47_04705"
     CDS             complement(7227..7907)
                     /locus_tag="PLR47_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HOG12274.1"
                     /translation="MNNQTTKTRRTGPDDRLIELQAKAALFNLLAQAFAYPDQLLMEK
                     LVSGEFAAILDESLGVLQPEGAWADRIAVVKRQYTAAGQNAADLLLELEKDYTRMFFS
                     SKPRLVYLFESVYKEGKLLQESTFEIARLYYEAGLNLVDDFKLPPDHIAVEFEFMAYL
                     YFNELAAVKSENRENEALAQKLQKEALEKHLTNFGRTLAEKVVTHAATDFYKIMAAVA
                     ASVLAAGR"
     gene            complement(8012..>8233)
                     /locus_tag="PLR47_04710"
     CDS             complement(8012..>8233)
                     /locus_tag="PLR47_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12275.1"
                     /translation="GYEENGGTHLFVLAKEDPVKFGYPKVAKKALKGKAALDGELGVP
                     ALAALAVVGFKKYSERRTRLETRTEKEKK"
BASE COUNT         1747 a         2307 c         2260 g         1919 t
ORIGIN      
        1 aacgtggttt tggaaatcac ggcgatcaga acggcggcgt ttttcgccca gattcggttg
       61 ccttccgcca gaagctcaaa gaaaacctcc cagcgcggcg tatcgcggcg ggcataaata
      121 aaccgccagg gctgattatt attggcggaa ggcgcccagc gcgccgcctc aaaaaggttc
      181 agaagcgccg cctcatcaag atccgcgccg gacatggccc ggggagacca gcgatgaaga
      241 aatatttcat gaacaccatt ttcctgctgt cgaaagtcct tgatattcat cgattcctcc
      301 ccattgccca actgccgtgg cgctgttttt taacaaagat taaccggttt tcttcagaat
      361 gcaagccttt ttaaaaatag tggagtctga gcttgacttt ccgggtttaa aaatatataa
      421 ggcgcaactt gctgaaaagc aggccctcta aaacagcgtc cccatcgtct agaggcctag
      481 gacacgggcc tttcacgcct gtaaccgcgg ttcgactccg cgtggggacg ccaatttggg
      541 gtcaaatctt tattcttgac atttgaccaa ctcattttga atttttatca attctccccc
      601 aagttccggg ttgttttcta ttttgatttg cagccgtttt cgtgcctggc tcaccgcact
      661 gtaatctacg ccgcccagga gcgatccgat ttccggctgc gtgagattgc aaatccggta
      721 taagatttcc atcagcatcg cccgctgggt cgattgtttg ccttttctgg tcaaatcctc
      781 tctgctgatt ttcaccagcc ttgcgtatcg atcaatcaaa tgatcgggtt taatctgttt
      841 tcccagcttt ctaagcgcag gctgctcccg ggaagaacgc tgatgcccct tgcttttgct
      901 gaatgtcttc tgaatttgtt cgataaaatc ctcgcctcca acaataccag taccctttcc
      961 cttctccagc ggactgggca ggtctctttc catgccttcc acgacaaatt tttgatattc
     1021 attcatggca ttgtgattct cgccgccaaa ataatccaga atgattttat attgaacaaa
     1081 gcttttcctg ttttgcggtt gaagatatcc cggaaaactg gtcgaatcat tgatcatcaa
     1141 atcccgaaat ctttgcccca cgggcttttg agacttaatt ctcagcggat tcaaatggat
     1201 gtagcgggaa acttccataa gataattgtt ttcatctatg agaaaagctt tgtacctccc
     1261 ctggtagaga tgcccggttc gtttatgttt ccggttaaaa aaaccggtat agctgatgtt
     1321 gaaatgacgc atgaattcgg aaaggttgcc tttcggtgtg caaacaagca ggtgaaaatg
     1381 gttgggcatc attgcatagg ccagtagttg aacctcaaag atagttaagg atcgactcag
     1441 caaacgaaag aattctttcc ggtcatcggc atgacgaaaa atttccctgc gttcattccc
     1501 gcggcaggtg acatgataaa aagcatccgg atattggatt cttaggggac gactcatggt
     1561 gttttttaat agagagagct tgaaatgtca agaataaaga tttgacccca ccctgcccca
     1621 agaataaaga tttgacccca ccctgccccc caccctgacc cttatcgcat tttctgtccc
     1681 gccgcatccg cggcgatcat ggcgtggagc acacgctcgg gagtgatgtc gcccgcttcg
     1741 tggtgaacag ccgcgccggg cgcacaggtc ttttccgccg cttttctcag tccgattcgg
     1801 tcccgtttat ccaatccgat atctgcaagc gtaacgggca atcccatttc cctgcaaaat
     1861 ccatagaccg ttcgcatcac ttccggtgat gcgcccgtca gatgaaggcc ggtcaatacg
     1921 ccgaaggcca ccttttcgcc gtgataatag gcattggttt cctcaagggc agtcaagccg
     1981 ttgtgaatgg catgagcggc ggcgagcccg gaactttcaa acccgattcc gctcagaaga
     2041 atattcgctt ccacgatgcg ctccagggcg ggagtcggca cctgatgctc gcaggcgagt
     2101 ttcgccgccg ctccacaggc caggagcgta tcgtagcata gcctggcgag atgcaggccc
     2161 gccattgtgc tcagtccgcc gcattcgttt ttcgattgcg tacggcggca ggattcagcc
     2221 tcaaaccagg tagccagcgc gtcgcccatg cccgccacca gaaaacgaac gggggccgcg
     2281 gcaataatgc cggtatctac caccactgcg gcgggatttg atttctggta gtattcccgc
     2341 tcgtggacgc cgttctcgga atagaccacc gcacagccgc tgcagggcgc gtcggtggag
     2401 gcaatggtgg gaacgatgat caccgggata ttcgcccggt ccgcggcaat tttcgccgta
     2461 tcgatcgttt tgccgccccc catgccgatc ataacgccga tgcggttctt ttcaatgacc
     2521 ttggccagac ggactaattc cttttcgcag cactccctgc cgaatgtttc gatgaacgcc
     2581 tggtttgttt cttcatgaat gccgcagacg ggaacaatgg ttttggctgc cgtgggggat
     2641 gcgaggacaa gcgccctggc gccgaagagt gagatcagtg ccggcaattc tttcagcgct
     2701 cccgcgccct gaatgtattt tccggggaat accgcttttt taaacatgcc tctcctccat
     2761 ttctcccatt tcgtatgtaa aaagataaat atcacaaaat ggccgcaatc ccatctgttt
     2821 ttatacttca aaagccattt gcgatgaatg gcgcaagatt ttcttgactt acaaagcctc
     2881 gttttttata ctccgcaaaa attatcagtt cacagtgaca aagacaaaga ttgcaataag
     2941 acagcctcct gatcatacat tcgtttattt gcggatggga agggcttcgc atgatcatgc
     3001 atggcgcaga taacgcccgt ttttggaccg atcagctgtt caacgcaatg attacggcgg
     3061 accgccggga ggcgtccggc gtaatggacc ggatgatggc cgccggtgta aattcccaat
     3121 cgatcattga tgacgttctc gatccgacgc ttatccggct gggcgacctt tgggagcagg
     3181 acgccgtatc gctcacccag accttcgtag cctccaagat cgccgaagat gtgctgcttc
     3241 gctgtgtgtc ggcttcagga aaaacggcaa agcggggcgc gccggttgtc atcggcaata
     3301 tcgaggatga tttccacagc ctgggacggc gcatcgtggg atcttttttg cgcgcgggcg
     3361 gctgggaggt tcacgacctg ggcaatgacg tgacggccga acagtttgtg gacaaggcaa
     3421 tcgaggttgg cgcgtccgtt gtaggggctt ccgcgatgat gcacaccacg gccctgaata
     3481 tccgcaaact gcgagatctg atcgacgccc gcgggctgaa tcatcagctg aagctggcgg
     3541 tgggcggcgc cgtcttcaac tggcggccgg atcttgtcgc ggaagtggga ggcgacggca
     3601 cggcccgcaa cgcaaccggg gttgatgctc tgtttcaaag gctccgcgac gcagcgaaag
     3661 gagggtgagc accatcaatt cgtgcgaacg cgtgatgaat acacttcagg gccttccgac
     3721 ggatcgcgtg ccggtctttg ccgtgctggg cgcctacggc ggaaagcttg ccgaaaccgg
     3781 cctgaaaacc ctttacagcg atgcagatgc ctacgtctcg ggacaacagg ccgtccagaa
     3841 tgccctgggt ttcgatatga ttctggccac cttcgattac agcgccatcg cagaggcgtt
     3901 cggcggcgag gtggcctggt ttgacaatca aacgcccaac atgaaacgcc ccgcggtcag
     3961 tgaagcctcc gcagttgccg gcctgaagct gcccgatccg cggcgagacg caaggctgcc
     4021 ggtcattctg tcggcaacgc ggcaactggt ctccctgtac cgggaaagtg tgccggtgtt
     4081 tgcggcggtg cctggtccgg gggcgctgcc ggcgctggtg ctcggactgg aaacgtggat
     4141 ggaaacggtt ttattcgacg aggataacgc ccgccgcatt ctggagcgga ccggccggtt
     4201 tttcctggac tgggtccatg cgctttgcga agcgggcgtg accggtcttg tggtgacgga
     4261 atccgtcgcc tccgtggaaa tttcgacgcg cgacctgttt gcgaaagttt tcctgcccca
     4321 cctgcacgat ctgttcggcc aaatcaacgc gcccctcgtg tttcaccacg gcggcggccg
     4381 aattctgcat attctcgact tgctgcccgg tttgccggga ttggcggggg tggtcgtcgg
     4441 ctccaaggat gatctgggcg ccgcaaggtc ggccctcgga accgaattga ccctgatcgg
     4501 caatctggat aatctgagcc tgccggccgc tacattctgt cgcactccgc cgcggatgtg
     4561 ccgctgagta caccgctgga aaacctgcag gccatgattg aggccgccgg ggaatatgcc
     4621 gccaaagaaa tgagtgcctt atgaatcctt ccaccatatg gttgagctgc ggcgttctgc
     4681 aggcggagct ggaacaattg catcgcgacg gccggattcg cggtgaactt cgattcctcg
     4741 actccatgct gcacatgaac ccagagcggc tgcaggcggc gctggaggcg gaagtagcca
     4801 agcttgtttc cgaaggttcc cgtgtggtgc tggtgtacgg cgattgctgc aaccggatga
     4861 cggatctgat ccgccggttc ggggtcagcc gggtcaacgc cgtcaactgc gcgcaaatgc
     4921 ttgcggggcg ccgccgctac cgtgaattga tgaagaatca ggcgtttatg ctcctgcccg
     4981 aatgggcgcc gcgctggaaa gaaatcatgc agggcgaact gggtttgtcc ccggaggtgg
     5041 cgcgcgatct catgcgggag aatcgccgcg agctggttta tctggatacg gcggtggcgc
     5101 cggcgccccg ggaagcgatc gaggaatgcg cgtcctatac gggattgccc ctgcgtgtcg
     5161 aaaaggtgtc gctcgatcat ctgctggccc ttttgctgca ggcggaatcc ggccgtatcc
     5221 ccccgcttcc cgaaaaggct gttgcgtctt ccgccccata ccgcaaaccg gcaacatggg
     5281 ccgatcaggt catgatggcg gatgtgtttt ccgaaatcct cgcatcctcc gatccttccc
     5341 ggctggccca aatcctgacg gaacaaatgc gcgaactcac cggcgctcat acggccatcc
     5401 tcgttgcgca tccggagggc gtccattcac accggatcat gcacgtgtcg cccgcccggc
     5461 gcggatcgct gttgacggcg gatgaagtcc ggcttttttg ttccgatctt gctcaggtga
     5521 tgcccctgta cgtcaaggat taccatcccg aacatccctg ccgtcttccg ctcttgcgcg
     5581 ccggcgtctc atcgctgatg cgctttccct tgcgggccgg agaagacctg ctggccacca
     5641 tgcttttgct ggatctgccg ggtgcggacc ggattgcaga gacggaaaaa atcatcaccc
     5701 atcttacgcc ggtcatggcg ctggccctga aaaattcctt tgcccgcgaa gaaattgaac
     5761 gtcaggcgaa gaaacttgcc tactatgcgg gcgacctgga gcgccgagtg gcccaacgca
     5821 ccgaggcgct cagggaaagc gaggaaaaat atcgcactct ctttgagaat gccggcgagg
     5881 ctatctttgt cgcccaggac ggcagggtgg tgtttctcaa tcccatgatg acccagatca
     5941 ccggttattc cggcgaggag cttgccgaca ggccgtttac cgatttcatt catgcggagg
     6001 accgcagcaa gatcttcgac cgtcataccc ggcgtcttca tggagaaaac attccccgga
     6061 gttacgcgtt tcgggcgttt cataaaaacg gcttcttgat ctgggtgcgg ttggacacca
     6121 ttctgatcga ctggcaggga aagcccgcaa cgctgaactt catggccgac atcacaaagc
     6181 gcaaacaggc ggaggaggcc ctgcaggacg cctataatcg tctcaataaa atcattgatt
     6241 ttctgccgga cgcaacgttt gtcgttgacc gcagcggaac cgtcatcgcc tggaacaggg
     6301 cgatggagca gatgtgcggg atttccaagg ccgaaatgat gggccacgga aatcatgaat
     6361 actccctgcc tttttatggc caacgcagac cgctgctcat tgaccttgcc ctgctgccgg
     6421 atgaagtgtt tgaaaagtct cattatgaga acattgcccg gcaaagcgac aatctgcagg
     6481 ccgaaagtta tgtgccggga gtatacggcg gaaaaagcgc gtttttgtgg ggtattgcct
     6541 ccagactgcg tgatgtttca ggaaatatca tcggcgccat cgagtccatc cgcgacatca
     6601 ccgaccggaa gaacagcatg gaccaactga gaaaggcgct gggcggaacc gttcaggcca
     6661 ttgccctggt ggtggaatcg aaagatcctt acaccgcggg ccatcagcgc cgtgtggccg
     6721 atatttcccg cgccattgcc acggaaatgg gcctctccca ggatcagatc gaaggcatcc
     6781 gcatggccgg catcatccat gacatcggca aagtctccgt tcccgccgaa attctcagca
     6841 tgcccaggaa attaacgcac ctggagttca gcctgattca aacgcatgcg caatccggat
     6901 acgatattct gaaagatatt gaatttccct ggcccattgc ccggatggtc ctggagcatc
     6961 atgaacgaat gaacgggtcc ggttatccca acggcttgac cggagacaag ctgcttttgg
     7021 agtcgcggat tctggcggtg gccgatgtgg tggaagccat ggcgacatac cgtccttacc
     7081 gggctgcatt ggggctttcc gccgctttgg aagagattgc ccaaaacaga ggcatcctct
     7141 acgacccggc ggcggtggat gcctgtctgc atcttttccg cgagaagggt tttacgatca
     7201 aagagtagtc aaaacctata ttttgcctac cggccggccg cgaggacgga ggcggcgacg
     7261 gccgccatga tcttataaaa atccgtcgcg gcatgggtga cgaccttttc cgccaacgtc
     7321 cgtccaaaat tcgtgagatg cttttccagc gcctctttct gaagcttctg cgcaagcgct
     7381 tcgttttccc tgttttcact cttcacggct gcaagttcgt tgaaatacag gtaggccata
     7441 aattcaaact cgaccgcgat gtgatcgggc ggcagtttga aatcatccac cagattgagc
     7501 cccgcttcgt aatacagcct cgcgatttca aaggtcgatt cctgaagcag ttttccttcc
     7561 ttgtaaacgg attcgaaaag ataaaccagc cggggctttg acgaaaagaa cattctggta
     7621 taatcttttt ccagctccag cagcaggtcc gcggcgttct gaccggcggc ggtgtattgg
     7681 cgcttcacaa ccgcgatccg atcggcccat gcgccttccg gctgaagaac gcccagcgat
     7741 tcgtccagga ttgcggcaaa ttcccccgaa accagttttt ccatgaggag ctgatccgga
     7801 taggcaaatg cctgcgccag caggttgaaa agcgccgctt tggcctgcag ctcgatcagg
     7861 cggtcgtcag gacccgtccg gcgcgttttg gtcgtttggt tgttcatgat tgcttcctaa
     7921 caaatttcca ccggattgtc caatgaccga caaaacggca aacgatccgg cggcgttgca
     7981 gagcgccgcc ggatttcagt ttagggttgg gttacttttt ttccttctcc gtccgtgttt
     8041 caagacgggt tcttctttcg ctgtacttct tgaagccgac aacggccaag gccgcgagag
     8101 ccggcacgcc cagttctcca tccagcgctg ctttgccctt gagggccttc tttgccacct
     8161 tgggatagcc gaacttcacg ggatcttcct tggccagaac gaacagatgg gttccgccgt
     8221 tttcttcgta gcc
//
LOCUS       DAOSIM010000074         8191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_8157_length_8191_cov_6.121804, whole genome shotgun sequence.
ACCESSION   DAOSIM010000074 DAOSIM010000000
VERSION     DAOSIM010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8191
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8157_length_8191_cov_6.121804"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /gene="lepB"
                     /locus_tag="PLR47_04715"
     CDS             <1..370
                     /gene="lepB"
                     /locus_tag="PLR47_04715"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOG12276.1"
                     /translation="DRSKDFIKRVIGVGGDKIEIRNKKILINDRPYKDKVGIYSDPVS
                     YPAIVQPRDNFGPATVPHNALFVMGDNRDQSLDSRFWGFVELKDVEGKAMVIYWSWNS
                     EEQDRFWQKIRWDRLGSPLH"
     gene            622..1164
                     /gene="pyrR"
                     /locus_tag="PLR47_04720"
     CDS             622..1164
                     /gene="pyrR"
                     /locus_tag="PLR47_04720"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOG12277.1"
                     /translation="MNNRPKIVMDSEGIERCLTRIAYEILEKNKGMDNLALVGIRTGG
                     IYLALRLQKRISAIENKKIPMGILDVTLYRDDVAKTSKKAPLGKTDIPFDLTDKKVIL
                     VDDVLFTGRTIRAAMDALIDFGRPKMIQLAVLIDRGHRELPIRADYVGKNLPSSLWEA
                     VSVNLLEKNGKDEVVIEEGS"
     gene            1188..2117
                     /locus_tag="PLR47_04725"
     CDS             1188..2117
                     /locus_tag="PLR47_04725"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOG12278.1"
                     /translation="MMKLAKKDILGIEEMTADEISLILETAESFLEISTREIKKVPTL
                     RGKSVINLFYEASTRTRTSFEIAGKRLSADTINIAASTSSAVKGETLLDTARTLEAMN
                     PDIIVLRHSVAGTPHMLARMVKQSIINAGDGAHEHPSQALLDMMTIREKKGRIAGLKV
                     AIVGDILHSRVARSNIYGLSRMGARVVVAGPATMMPRGIERMGVEAFTTIEDAIAGAD
                     VVMMLRIQLERQKQNIFPSLREYSHHYCLSRRHMKLTAPDAIVMHPGPINRGVEISPD
                     IADDPACSVILDQVNHGVAVRMAMLYLLSGGNE"
     gene            2114..3403
                     /locus_tag="PLR47_04730"
     CDS             2114..3403
                     /locus_tag="PLR47_04730"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOG12279.1"
                     /translation="MKLLLKGARVIDPAQNLDALMDVLVEGGKIAACKPGIKAPAGAK
                     VLELSGMLVTPGLIDMHTHLREPGYEYRETIASGSAAAVAGGFTSIACMPNTLPVNDN
                     RSVTEFILRKAAEANLANIYPIGAISMGSEGKQMTEFWDLREAGIAALSDDGNPVMDA
                     ALMRRAMEYAYSLELPVIQHCEDKNLSAGGLMNEGYQATVLGLPGIPAIAEEVMVARD
                     ILIAEYTGTRIHFAHVSTTGSVRLIRAAKKRRLKVTAETAPHYFTLTDESLRNYDTNY
                     KVSPPLRGDADVSAIKEGLADGTLDAIACDHAPHGRTDKEVEFEYAACGISGLETSLG
                     LSLSLVQEGILPWPELIAKMSCNPARILNLPKGTLAKGADADITVIHPDLAWSVDVAA
                     FHSLGKNSPFSGRKLKGRAVLTIVGGDIKYDGRASQP"
     gene            3400..4173
                     /gene="recO"
                     /locus_tag="PLR47_04735"
     CDS             3400..4173
                     /gene="recO"
                     /locus_tag="PLR47_04735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOG12280.1"
                     /translation="MSARETHKTAGFVLRSLQYGESDLIVTFYTREFGKIKGIAKGAK
                     RSRKRFANVFEPFSLTQIIFSRRNRDSLAFIDACDILDHFAAIRMDLEKTLTASYFID
                     LAEHFSPEGKRNDGVFALLLDFLPLLTRENVSEPIIRFFEMRLLKAAGFEPELAACVR
                     CKTPVANGSPYYFFPKEGGIFCTACARPQRYDQSISAGAVRTLLLGKDMDLEKMKMIS
                     MTESLASESRSILCGFISYVLGREVQSLKVLEQVRRYCP"
     gene            4187..5446
                     /locus_tag="PLR47_04740"
     CDS             4187..5446
                     /locus_tag="PLR47_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HOG12281.1"
                     /translation="MRRPELLAPAGNLEKLRTALLYGADAVYAGVPGLSLRGAPAEMS
                     LEDLASGVSEAHAQGIRVYAAVNTFARNADLKQARLHLPDVAATGVDALIVSDPGLIR
                     LARRTVPQIPLHLSTQANTTNADAVRFWRDLGVSRIILARELPLGEVEEIASALPDVE
                     LEIFIHGAMCMAYSGRCYLSAWRNRRSANEGDCTQPCRWEYRLSESTRPEDPIVVEED
                     ERFSYLLSSKDLCLLEHLPEVLATGVAGVKIEGRMKSAYYAAVVTRTYRQALDALCRD
                     KEAYRCDPRWIEELKTVSHRGYTTGFAFAEEKINESSPDVKNIQTHEPAGMVVGYDGI
                     RKQMRMEVRNFLQAGEEMELLLMEETLPVSAQPMTDDRGSPIDRAHAGNLIRVPLPFP
                     APTGALLRKKTVGAKTEETKRKPSENG"
     gene            complement(5455..6084)
                     /locus_tag="PLR47_04745"
     CDS             complement(5455..6084)
                     /locus_tag="PLR47_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12282.1"
                     /translation="MKRIHLVLYFFLIVFLLAGCAKGPSVTKKEEPKLKETPPAPVVV
                     KKPPVAPPPPAPAIEKLPEIARSIPDSKVWVRYGKTKMGENYYNKTNITRSSDVATVN
                     VYRVVTDSCRKQMVEEVRKYNPKKSIKYHYYEHDVRVDEIDCRNQKWRVKELIHYDEK
                     GKVLDHHTYENEPWEKIKFVTDHHTLQKSICVAEEKPAAKKPAAKKKKK"
     gene            complement(6353..7447)
                     /locus_tag="PLR47_04750"
     CDS             complement(6353..7447)
                     /locus_tag="PLR47_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG12283.1"
                     /translation="MKKFASLVVVLLVMLLVSTGNAQVRSGSFTVAPTLGMYVFEGNE
                     DMDRAMPLVGMRLGYNFNKYLGVEGFVHYAKTDNNAWSPKQNVDFTGYGAEVIFNLLP
                     NQALVPFLAAGVGGVHYSYGVFEDDSDYGTDNYKKDKLAFDYGYGLKWFLSDWFALRL
                     DIRHVLPIEKLHNNMLVSLGLNFAFGGAKKAVATPVSAAEPVMEEAKPQAEAAPVVEE
                     AKPAVAAAAPVVTEEVVRPQPRTQVREEVDEMGRATLEVLFDFDKATIKKNYTKDIDH
                     MVGVMKEHSDVNLTIEGHTDNVGKAEYNKNLSQKRAEAVKNYMVEKGGIDAQRLNAVG
                     YGQEKPVASNKTKAGRAKNRRVEAAVDYKK"
     gene            complement(7655..8047)
                     /locus_tag="PLR47_04755"
     CDS             complement(7655..8047)
                     /locus_tag="PLR47_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOG12284.1"
                     /translation="MKWYHVGIKAAKSEASVRFYRDILGLEVIDRVEIWGKQFNFVGN
                     DTISIEIEQANPGDTPSDPSSMTGLNHFSLVVDDIHALVEQARKKGAGVLMDPFHPRP
                     DRLTTFIRDPDGVMIQLIQFVSKDPAGG"
BASE COUNT         1896 a         2358 c         2170 g         1767 t
ORIGIN      
        1 ggaccgctcc aaggacttca ttaaacgggt catcggcgtg gggggcgaca aaattgaaat
       61 ccgcaataaa aaaatcctga tcaacgaccg gccctacaag gacaaggtcg gcatctattc
      121 cgatccggtc tcctacccgg ccatcgtcca gccccgcgat aatttcggcc cggcaacggt
      181 ccctcacaac gcccttttcg tgatgggcga caaccgcgat cagagtctgg acagccggtt
      241 ctggggtttc gtggaattga aagatgtgga gggaaaggcg atggtgattt actggtcctg
      301 gaacagcgag gaacaggacc ggttctggca aaaaatccgc tgggatcgcc tgggaagccc
      361 gctgcattga cgcccggccg cgcgtatttt tatcttgaca gtcagccccc caggttgtat
      421 gaacgcggca agaaaacttt taattcgatc ccgtgagatt gaaaaagaag aaaaaaatga
      481 aaacaatttc ccataagccc atgaaagcct ttctccgcaa ggggaaaggc tttttttata
      541 accgtcgcgc atgctttccg aacacgcgct gatcctcagg caagccgtag cggcggatga
      601 aaaaacgaaa ggggcgtcaa catgaacaac cggccgaaaa tcgtgatgga cagcgaaggg
      661 atcgaacgct gcctgacgcg catcgcctat gaaatcctgg aaaaaaacaa ggggatggac
      721 aatctggcgc tggtgggcat tcgcaccggg ggcatttatc tggccctgcg tctgcaaaaa
      781 aggatttcgg ccatcgaaaa caagaaaatc ccgatgggca ttctcgatgt gacgctttac
      841 cgcgatgatg tcgccaaaac cagcaagaaa gcgccgctgg ggaaaaccga cattcccttt
      901 gatctcacgg acaaaaaagt cattctcgtg gacgatgtcc tcttcaccgg gcgaaccatc
      961 cgcgccgcca tggacgccct gatcgacttc ggccgtccga aaatgattca actggccgtc
     1021 ctgatcgacc gcggacatcg ggaactgccg attcgcgccg attacgtcgg gaaaaacctc
     1081 ccctcctccc tgtgggaagc ggtgagcgtc aatcttctgg aaaaaaacgg caaggatgaa
     1141 gtggtcatcg aagaagggtc ataaacctga acctgcaagg caatcgcatg atgaaactgg
     1201 caaaaaaaga catcctcggc atcgaggaaa tgaccgcgga cgaaatatcc ctgattctgg
     1261 aaacggccga atccttcctg gaaatttcca cgcgcgaaat caagaaagtt ccgacgctgc
     1321 gcggcaaaag cgtgatcaac ctgttttatg aagccagcac gcgaacccgg acgtcctttg
     1381 aaatcgcggg caaaagacta agcgccgaca ccatcaacat tgccgcgtcc accagctccg
     1441 cggtcaaggg cgaaaccctt ctggataccg cgcgtacgct ggaagccatg aatcccgaca
     1501 ttatcgtgct gcggcacagc gttgccggaa cgccgcacat gctcgcccgc atggtgaagc
     1561 agtccattat caacgccggc gacggcgcgc atgagcaccc ctcacaggcg cttctggaca
     1621 tgatgacgat ccgggagaag aaaggccgga ttgccgggct gaaagtcgcg atcgtcggcg
     1681 atattctgca cagccgcgtc gcacggtcca acatttacgg cctttccagg atgggcgccc
     1741 gtgtggtcgt ggccggaccc gccaccatga tgccgcgcgg catcgaacgg atgggggtcg
     1801 aagccttcac gaccatcgaa gatgcgatcg ccggcgccga cgtcgtcatg atgctgcgca
     1861 ttcaactgga gcgccagaag cagaacatct tcccctcgct tcgcgaatac tcccatcatt
     1921 actgtctcag ccgccggcac atgaagctca ccgcacccga cgccatcgtc atgcatccgg
     1981 gtccgatcaa ccgcggcgtg gaaatctctc ccgatatcgc cgacgacccc gcctgctccg
     2041 tcattctcga ccaggtcaac cacggcgtgg ccgtccggat ggccatgctg tacctgcttt
     2101 caggaggaaa cgaatgaaac tgctgttgaa aggcgcccgg gtgattgacc cggcacaaaa
     2161 cctggacgca ctgatggatg tgctggtgga aggaggaaaa atcgccgcct gcaaaccggg
     2221 catcaaagcc ccggcgggcg ccaaagttct cgaactgtcc ggaatgctgg tcacgcccgg
     2281 actgatcgac atgcacacgc atctgcgcga accgggatat gaataccggg aaaccatcgc
     2341 cagcggctcc gccgccgctg ttgccggagg attcacttca attgcctgca tgcccaacac
     2401 cctgccggtg aatgacaacc gctcggtcac cgaattcatc ctgcggaagg ccgccgaagc
     2461 gaatctggcc aatatctatc ccatcggcgc gatcagcatg ggctccgagg gcaaacaaat
     2521 gaccgaattc tgggacctca gggaggcggg catcgccgct ctgtcggatg acggcaaccc
     2581 ggtgatggac gcggccctga tgcgcagggc catggaatac gcctattccc tggagctgcc
     2641 ggtgatccag cactgcgaag acaagaacct ctcggcgggc ggcctgatga atgaaggata
     2701 ccaggccacg gtcctggggc tgccgggcat tcccgccatc gccgaagaag tcatggtggc
     2761 gcgcgacatc ctcatcgccg agtacaccgg cacgcgcatt cacttcgcgc acgtgagcac
     2821 aaccggctcc gtccgcctga ttcgcgccgc caaaaaacgg aggctgaagg tgacggcgga
     2881 aaccgccccg cattatttca ctctgacgga tgaatcgctt cggaattatg acacgaatta
     2941 caaagtcagt ccgccgctgc ggggtgacgc cgatgtgtcg gccatcaagg aaggcctcgc
     3001 ggacggaacg ctggacgcca tcgcctgcga ccacgcgccg cacggccgta cggacaagga
     3061 agtggaattc gaatacgccg cctgcggaat cagcggcctg gaaacctctc tgggcctgag
     3121 tctgagcctg gtgcaggagg gaattctgcc gtggccggag ctgattgcca aaatgagctg
     3181 caatccggcg cgcattttga acctgcccaa agggacgctg gcaaaaggcg ccgacgcgga
     3241 cattacggtg atccatccgg accttgcctg gagcgtcgat gtcgccgcct ttcattcact
     3301 gggaaagaac tcaccgtttt ccggaagaaa gctgaaaggg cgcgccgttt taaccatcgt
     3361 gggcggcgac atcaagtacg atgggcgggc atcccagccg tgagcgcccg ggaaacccat
     3421 aagaccgccg gctttgtcct gcgaagcctt cagtacgggg aatcggattt gatcgtcacg
     3481 ttttacaccc gtgagttcgg caagatcaaa ggtattgcca agggcgccaa acgcagccga
     3541 aaacggttcg ccaacgtctt tgaacccttc tccctgacgc agatcatttt ttcccgccga
     3601 aaccgtgact cgctcgcgtt tattgacgcc tgcgacatcc tcgatcactt tgccgccatc
     3661 cgcatggacc tggaaaagac cctgaccgct tcctatttta ttgatctggc cgaacacttc
     3721 agcccggagg gcaagcgcaa tgacggggtc tttgcattgc ttctggattt tctgccgctt
     3781 ctgacccggg aaaacgtatc ggagccgata attcgtttct ttgaaatgcg ccttttgaaa
     3841 gccgccggtt ttgagcccga gcttgcggcc tgcgtacgct gcaaaactcc cgtggccaac
     3901 ggttccccct actacttttt cccgaaggaa ggcggcattt tctgcaccgc atgcgcaaga
     3961 ccccaacgct acgaccagag catctccgca ggcgccgtcc gcacgcttct tctgggaaaa
     4021 gacatggacc tggaaaaaat gaaaatgatc tcgatgacgg aaagtcttgc atccgaatcc
     4081 cgcagtattc tctgcggctt catttcctac gtgctgggaa gggaagtcca gtctctgaaa
     4141 gtcctggagc aggtgcgcag gtactgtccc tgacggaggt ttttttatgc gccgaccgga
     4201 actgctggcc cccgccggca acctggagaa actgcgcacc gccctgctgt atggggcgga
     4261 cgccgtctat gccggagttc ccggcctgag cctgcggggc gcgcccgccg aaatgtcgct
     4321 ggaggatctg gcctcgggtg tttccgaagc gcacgctcaa ggcatccggg tctatgccgc
     4381 cgtcaacacg tttgcccgca atgccgatct gaagcaagcc cggctgcatc ttccggatgt
     4441 ggccgcaacg ggggtggacg cgctgatcgt cagcgacccg ggcctgatcc ggctggcccg
     4501 aaggactgtt ccgcagattc cgcttcatct cagcacacaa gccaacacca ccaatgccga
     4561 cgcggtccgc ttctggcgtg acctgggcgt gagccggatc attctcgcac gggagcttcc
     4621 ccttggggaa gtggaggaaa tcgcctcggc tctccccgat gtggagctgg agattttcat
     4681 ccacggcgca atgtgcatgg cctattccgg acgctgttat ctgtcggcct ggcgaaaccg
     4741 caggagcgcc aacgagggag actgcaccca accctgccgc tgggagtacc ggctttcgga
     4801 atccacccgc ccggaagatc ctattgtggt ggaagaagac gaacggttct cgtatctttt
     4861 gagttcaaaa gatttatgtc tgctggagca tctgcccgag gtcctggcga ccggcgtcgc
     4921 cggcgtcaag attgagggaa gaatgaaatc cgcctattat gcagccgtcg tgacccgaac
     4981 ctaccggcag gcgcttgacg ccctttgccg tgacaaagaa gcctaccgct gcgatccgcg
     5041 gtggatcgaa gagctcaaaa ccgtctctca ccggggctac acgaccggat tcgcctttgc
     5101 cgaagaaaag atcaatgaat ccagtccgga tgtcaaaaac attcagacgc atgaaccggc
     5161 ggggatggtg gtcggctatg acggcatcag gaaacaaatg cggatggaag tccgcaattt
     5221 cctgcaggcg ggcgaagaga tggaattgct gcttatggag gaaaccctgc ccgtctccgc
     5281 ccagccgatg acggatgacc ggggaagccc aatcgaccgg gcccacgcgg gaaacctcat
     5341 tcgcgttccc cttcccttcc cggcgccgac cggcgctctg cttcggaaaa aaaccgtcgg
     5401 ggcaaaaacg gaagagacaa agcgcaaacc ttctgaaaac ggctgacgat cagatcactt
     5461 ctttttcttc tttgctgcgg gcttttttgc ggcgggcttt tcctcggcga cgcaaatgct
     5521 tttctgcagc gtatgatgat ccgttacaaa tttaattttt tcccagggtt cattttcgta
     5581 ggtgtgatga tccagaacct ttcccttttc gtcgtaatgg atcagctctt tcacccgcca
     5641 tttttgattc ctgcaatcaa tctcatccac gcggacatca tgttcgtagt aatgatactt
     5701 gatggatttc ttcgggttgt atttcctgac ttcttcaacc atctgcttcc tgcaagaatc
     5761 cgttacgact ctgtaaacgt taacggttgc cacatcggag gatctggtaa tattggtttt
     5821 attgtaatag ttttctccca ttttggtttt tccatagcgc acccatacct tgctgtccgg
     5881 aatgcttcgc gcaatttcgg gcagtttttc gatggcggga gctggaggag gcggggcaac
     5941 ggggggtttt ttcacaacga ccggggccgg gggagtttct ttgagtttcg gctcttcttt
     6001 tttggtaacg gacggacctt tcgcgcagcc cgcaagcaga aaaacgatca gaaaaaaata
     6061 taaaaccaaa tggatccttt tcatgtctgc tcctttcttc attcttccat gccccggcca
     6121 atccggtcat ccctcgtcca cgagcggcaa tgaaagcggt cctgataaaa ttgcttgtga
     6181 aatttaatac atttatgatg atttatcaag gatctctcac atccagcctg aaaaaaatgg
     6241 tcccccgccg ggtgcgccaa acccggtttc ccaataaaga aagccccccc ggcaaaaccg
     6301 ggggggcctc attggaaccg aaagcctttc agacgaaaga cttcatcatc tgctatttct
     6361 tataatcgac ggccgcttcc acgcggcgat tctttgcgcg ccccgctttg gtcttgttgg
     6421 aagcaacggg tttttcctgg ccgtatccga cggcgttcag acgctgggcg tcaatgccgc
     6481 ctttttcaac catgtaattc ttaacggctt ccgcgcgttt ctgggaaaga ttcttattgt
     6541 actcggcctt gcccacattg tccgtatggc cttcgatggt gagattcacg tcagaatgtt
     6601 ctttcatgac accaaccatg tggtcgatat ccttggtgta attctttttg atggtggcct
     6661 tgtcgaaatc aaacaggacc tccagcgtag ctcttcccat ctcatcgact tcttcgcgca
     6721 cctgagtcct gggctggggt ctcacgactt cctcggtaac cacgggggct gccgccgcga
     6781 cggcgggttt cgcctcttca actaccggtg cggcctccgc ctggggtttt gcttcttcca
     6841 taaccggctc ggctgcggaa acaggcgttg caactgcttt tttggcgcct ccaaaagcaa
     6901 aattcaaacc gagagatacg agcatgttgt tgtgaagctt ttcgatcggc agcacatggc
     6961 gaatgtcaag gcgaagcgcg aaccaatccg acaggaacca cttcaaaccg tagccataat
     7021 caaaagccag cttgtctttt ttgtaattgt cggtcccgta atctgaatca tcctcgaata
     7081 caccgtagga atagtgaacg ccgccaacac cggccgccag gaaaggcacc agcgcctgat
     7141 tcggcagaag attgaaaata acctctgcgc cgtatcccgt gaaatccaca ttctgcttgg
     7201 gagaccaggc gttgttatct gtcttggcat aatggacaaa accttcgacg ccgagatatt
     7261 tgttgaaatt gtagccaagg cgcatcccga ccagcggcat tgccctatcc atgtcttcgt
     7321 tgccttcaaa cacatacatg cccaaggtcg gcgcaaccgt aaaagacccg gacctcacct
     7381 gcgcgttgcc tgtggagacc agcaacatca ccagcaggac aacaaccaat gaagcgaatt
     7441 ttttcataat ctttctcctt aaaataaacg tattttgaat tgcactcgaa ctttgtttaa
     7501 tcaggccaac ccgcccatat tccaaacaaa caattaaaaa ctttcgaatt tactgacaca
     7561 ttgtgaccaa tgcttctata atcaatcact taatgtatgt caatataatt tttcatttat
     7621 atttatcacc gaaaaacgcc tgatccttcc cggtttaccc tccggccggg tcttttgaaa
     7681 caaattgaat cagctggatc atcacaccgt cgggatcccg gataaaggtc gtgaggcggt
     7741 ccggccgggg atgaaacgga tccatcagga cgccggcgcc ttttttgcgg gcttgctcca
     7801 ctaatgcatg aatatcgtcc acgaccaggc tgaaatgatt gagaccggtc atggaagaag
     7861 gatctgatgg ggtgtcgccg ggattggcct gctcaatctc gatggaaatg gtgtcatttc
     7921 ccacgaaatt gaattgtttt ccccagattt ccacccgatc gatcacttcc agacccagaa
     7981 tgtcccggta aaatcggacg gatgcctcac ttttggcggc tttgattccg acatgatacc
     8041 atttcatatc agcctccaaa cttttctttt gcaagtttga aacactcgga ggccacatca
     8101 atcgtcctga gaatatccga ttccgtcatg gccgcgcaga caaaccagtt gtggtgcgga
     8161 tggaaaaaga tgccgcggcg ggccgcctca c
//
LOCUS       DAOSIM010000075         8098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_8273_length_8098_cov_7.587467, whole genome shotgun sequence.
ACCESSION   DAOSIM010000075 DAOSIM010000000
VERSION     DAOSIM010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8098
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8273_length_8098_cov_7.587467"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            557..3463
                     /locus_tag="PLR47_04760"
     CDS             557..3463
                     /locus_tag="PLR47_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOG12285.1"
                     /translation="MTKKCFLKMVFSLLLTAVIAGLAPQDIFSQDRMGARGGRTIPMD
                     RPAVAVPGANAGTASSTAQATAAPAGKTWFGATVRPVTGKDMRIMHMEYGRWPVVFVS
                     EVVKESPAEKMGIRLNDIILSVNYKDIKTPGEFQDAVAALRPGDKIVLLVAHAGEKAQ
                     YISGTAESPPGGAKTAAAEKAVPAASPAVTAADIAAKIFAPTGSDTINAIRCSPDGKY
                     LISGGGYKKDSLNLWDISAGKKIRAYRGHADAVNAVAFSPDGKTILSGSFDKTVNCWD
                     VDAEQALWTFSGHEALILDVTFSPDGKYAASGSRDNTVKLLDAASGREVRTFSGHTDG
                     VESIVFSPDGRYLLSGSFDKTLILWDVQTGDRLQTFEGHQWQIASVAFSPDGRQIFSR
                     SGDRVVKSWDVAAGREIRTFTADDVNSAGAKSGSQGIRAGAFSADTRRMITTKSFGGA
                     ISLWDMDSGRILKKFAGHLGNVPAVAFLHNERYFLTGGQDGAIRIWDIDAGREIVQFV
                     SLKDGEWIVLTPEGYYNSSLNGHKYLHIKVGGKVYGIDQFYDVFYRPDIVSAKIKGED
                     ISSLVSLTIDEALKNPPPAVEFTTVPPQTDSPKVKVCYQAESTGGGIGEVRLFHNGKL
                     IQSDGYYREVAKTSTGTTSLASLNSRSIYADMRSINIKAKTDAAFIASQAKGDRFEDC
                     REIDAIAGENEVSVSAFNAGNTVQSYLKTITFNSGIKPEDPHLYIFSIGIDQYKDSSV
                     NLKYAVKDAKELEAKIKAQSATLYRPQNIHYSLVTDQEANKTNIINKINALADTIKPQ
                     DSFILFVAGHGVLLQNQYYMLTHDFDGRINDAGVISSNEIVEMSKQIKALSQLFIFDT
                     CHAGGVDTIISGLYDARMSVLARKMGLHIFASANDQQAAMDGYKGNGLFTYTLLDGLN
                     NNKEADKYKDGKVSIVGLGEYARKMTTAISKQIRHEQTPLIINFGKDSPLYQVR"
     gene            complement(3712..5706)
                     /locus_tag="PLR47_04765"
     CDS             complement(3712..5706)
                     /locus_tag="PLR47_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOG12286.1"
                     /translation="MDIIVETRASRITVQCSGRLDVASAKEFETTLEPLVKKSDKQVV
                     LDFSQLQYISSAGIRSLLFLMKTAAASSAAGQTRNLLPFRLIQASPAIRQVLELSGLL
                     ESFSSGFRATPGTKPVQGKTYAELLPLIRGAFPSPVSNADADRHFVAAVAGGLEQIDK
                     LKTDRPYLGRRETIDYSQARSRQIPENMSGVAETVADIAEYMQGGFIWSHPHTQENVV
                     PPTTVPSIIGNLFGAIYNPNVISDEYSYRVGLAEIETAAMCAALVGYDPQSAAGFFTF
                     GGTGTFLYGAKIGVEKASPGTFGRGLHEPLKLVASSAAHYAKINVLGWLGVGTDNLVS
                     VPTDADNSMDLAALETNLRRILDSGEKIAAIIATMGTTDAFGIDNLQYIAGLRDSLAA
                     EYQLPYLPHIHADAVIGWAWSVFNDYDFDANTLGFNARTLRCLWDTHVNISALSRADS
                     VGIDFHKTGFGPYVSSMFLCKNKDDLSLISRDAGLMPYLFQFGNYHPGIFTLETSRAG
                     GAALASLANMKLFGKEGYRGLLGHLVTMAEVLRKRLDEHPAMCQVNDYNYGPVTLFRV
                     YPDGMNAKAVFYDELNSPEAVESLRRSNVYNRKVFDELHRQMEQENGFALSVTAHYRT
                     AACGEPVLAVKSFVMSPFIEERHMQDLIECLEKARRTVGY"
     gene            complement(5758..6333)
                     /locus_tag="PLR47_04770"
     CDS             complement(5758..6333)
                     /locus_tag="PLR47_04770"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOG12287.1"
                     /translation="MKVLGINGSPHKNGNTSLLIKTVFQELKNEGIETELIHIAQKPV
                     RGCKGCLKCVKNQNKQCVFDDDIMNECIGKMTAADGIILGSPTYFTDVTAEMKALIDR
                     AGMVSIANGGIFKHKAGGSVVAVRRGGATHAFDTMNHFLHYMQMFLVGASYWNMVYGW
                     APGEVNNDQEGIENMKTLGKNMAYLLKKLSM"
     gene            6620..>8098
                     /gene="gpmI"
                     /locus_tag="PLR47_04775"
     CDS             6620..>8098
                     /gene="gpmI"
                     /locus_tag="PLR47_04775"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOG12288.1"
                     /translation="MSLHLNKLQSFRGRKGPLLLIIMDGIGLGKADESNAVFRANTPN
                     LDELFKSKLYAQLSAHGTAVGLPSDDDMGNSEVGHNAMGAGRIFDQGARLVNAALQTG
                     RIFQTEVWDSIARRAQDGGTVHFIGLLSDGNVHSHIDQLYILIDQCASLGFKKVRLHT
                     LLDGRDVGEKTALNYLGPTEEKLKKISAEKGLDYAVASGGGRMTTTMDRYNSDWSIVK
                     RGWEAHVFGKGRTFPSASDAVRTFYAEDPKATDQYLPAFVVTDGSGRAVGKIQDGDAV
                     ILFNFRGDRAIEISRAFSEKDFSEFDRGPLPNVFYAGMMEYDGDAHIPPNFLVQPPAI
                     DRVIGEYLCAEKVKTFAVSETQKYGHVTYFWNGNRSGCIDEALEKYVEIPSDRIEFDR
                     APKMKAKEITDMVIDLLQSGNYRFGRTNYPNGDMVGHTGVTEAAIVAVEAVDQCVGKL
                     LDVIRKLGGIAVITADHGNADQMFTIDKKGVKSVKTAHSLNPV"
BASE COUNT         1944 a         2191 c         2143 g         1820 t
ORIGIN      
        1 gacgccatcg tgtgatgtaa aatggagcgc tatgccggaa ggaaccaacc atacgccagc
       61 gcaaaacaaa gccaccgatg ccaatacgcg aatattgaat cttgaccgat cagcaacttg
      121 attccccatc ttttcaatcc tcttcctttc cattttttcg gccgagccat caatacaggg
      181 tgtttggttt ccttgactgt tcacagcctt tgttcgctta ttgaaaggta cgtctttgca
      241 aggcaaacgt caatgatttt aggccgacat ggtaatcaca ggatcaaccc ttttgtggag
      301 catcgcgaac ttttggcaga ccttttaatg atttctcact tgcgattcaa tgaaaataga
      361 accgtcccct ttttttactt tttttattcg gcttcccttt ttcatgcagg aagtgtacag
      421 acgcagatgt ctgcatccct gtcctggata cagaccgcgt gtaaaattga agatttttaa
      481 aagccaaaaa tctggccacc gtccccaacc cgattggttt catcccggag gtcagtctat
      541 tgattgagga acccatatga caaagaagtg ttttctgaaa atggttttca gtctgctttt
      601 gacagcggtg atagccggcc ttgcgccgca ggatattttt tcgcaagacc gaatgggcgc
      661 aagaggaggc aggaccattc cgatggaccg gcccgccgtt gccgttcccg gagccaatgc
      721 gggcacggcc tcttcaacgg cccaagccac ggcggccccc gcgggaaaga cctggtttgg
      781 cgcaacggtt cggccggtca cgggaaaaga tatgcggatc atgcatatgg aatatggaag
      841 gtggccggtt gtttttgttt cagaagtggt caaagaaagt ccggcggaaa aaatgggcat
      901 tcggctcaac gatatcattc tttccgtcaa ctacaaggac atcaaaacgc ccggcgagtt
      961 tcaggatgcc gttgccgctt tgcgtcccgg agataaaatc gtcctgctgg tcgcgcacgc
     1021 cggcgagaaa gcgcaataca tatccggaac ggcggaatct cccccaggag gggctaaaac
     1081 ggcggcggct gagaaagctg tcccggcagc ctcccccgcc gttacggctg ccgatatagc
     1141 ggcaaaaatt ttcgcgccca cgggcagtga cacgatcaac gcaatccgtt gctctcccga
     1201 cggcaaatac cttatttccg gcggggggta taaaaaagat tcccttaatc tctgggatat
     1261 ttccgcgggc aaaaagatcc gcgcgtaccg ggggcacgcg gatgccgtca acgccgtggc
     1321 tttttctccc gacggcaaaa ccatcctttc aggaagcttt gataaaaccg tgaactgctg
     1381 ggatgtcgac gcggaacagg cgctatggac tttttccggc catgaggcgc tgattctgga
     1441 cgtcaccttt tctccggatg ggaaatatgc cgcgtcaggc agccgggaca acaccgtcaa
     1501 actcctggac gccgcttcag gccgggaagt cagaaccttt tcggggcaca cggatggcgt
     1561 cgaatccatc gtgttttcac ccgacggcag atatcttctt tcggggagct ttgataagac
     1621 gctgatcctg tgggacgtcc aaaccggcga caggttgcag acctttgaag gacaccagtg
     1681 gcagatcgcc tccgtggctt tctcgccgga cggcaggcag attttctcga ggagcggaga
     1741 tcgggtggtt aaaagctggg atgtcgccgc aggccgggaa atcaggacat ttaccgcgga
     1801 tgatgtcaat tccgccgggg cgaaaagcgg ctcgcaggga atacgggctg gagccttttc
     1861 cgcggataca cgccgcatga ttaccacaaa gtcttttggc ggcgccattt cactctggga
     1921 tatggattcc ggcaggattc tgaagaagtt tgccggtcac ctgggaaatg tccctgccgt
     1981 agccttttta cataatgaac gttattttct taccggggga caagacggcg cgataaggat
     2041 ttgggacatc gatgccggca gggaaatcgt ccagttcgtc agtttaaaag acggcgagtg
     2101 gatcgttctc acgcccgaag gctattacaa ttcatccctg aacggccata aatatctcca
     2161 catcaaagtg ggcggcaagg tctatggaat cgaccagttc tacgatgttt tttaccgccc
     2221 ggacatcgtg tcggcgaaga ttaaaggcga ggacatcagc agcctggtca gcctcacaat
     2281 tgacgaggcg ctgaaaaatc caccgcccgc ggttgaattt acaaccgtgc cgccgcagac
     2341 cgactcgcca aaggtcaagg tatgttatca ggccgaaagc acgggcggcg gcatcggcga
     2401 ggtgcgatta tttcacaacg gcaaactcat ccaatcggat ggttattacc gggaagttgc
     2461 caaaacatca accgggacaa cctcgttggc ttcgctaaac agccggtcta tctacgcgga
     2521 tatgagaagc atcaacatca aagccaagac agacgctgcg tttatcgcca gtcaagccaa
     2581 gggagatcgc tttgaagact gcagggaaat cgatgcgatt gccggcgaaa acgaggtttc
     2641 cgtctccgct tttaacgccg gcaacaccgt ccagagctac ctgaaaacca ttacattcaa
     2701 ctccggaatc aagccggagg atccccatct ttatattttt tccatcggga tagaccaata
     2761 caaagacagc agcgtcaatc tcaaatacgc cgtcaaggac gccaaagagc tggaggcaaa
     2821 aatcaaagcc cagtccgcca cgctgtatcg gccccaaaac attcattatt cgctggtgac
     2881 ggaccaggag gccaacaaga caaatattat caataaaatc aatgcgctgg ccgacaccat
     2941 caagccacag gacagcttta tcctctttgt ggccggccac ggcgtcctgt tgcagaatca
     3001 gtattacatg ctcacccatg atttcgatgg ccggataaat gatgcgggcg tgatcagctc
     3061 caacgaaatt gttgaaatgt ccaaacagat taaagcctta agccagttgt tcatcttcga
     3121 tacctgccac gcgggcggcg tggacacgat catcagcggt ctttatgacg cccgaatgtc
     3181 cgtgctggcc aggaagatgg gcctgcatat tttcgcctcg gccaatgacc agcaggcggc
     3241 gatggacggc tataagggaa acggcctttt cacttacacc ctgctggatg gcttgaacaa
     3301 caataaggaa gccgacaaat ataaagacgg gaaggtcagc atcgtgggtt tgggagagta
     3361 tgcaaggaag atgaccacgg caatctctaa gcagatccgc catgaacaaa cgcctttgat
     3421 catcaatttt ggaaaagaca gtcctttata tcaggtgcgg tgagccgcga aaggctctct
     3481 gggcggcgcg cccccggccc tgaccgaagg gccgccttga tcaactttta tattttacat
     3541 gcagtccatc agtatgacca ttcagaatgg gcaagcatta ttcccaaaag cggctcagaa
     3601 cgactatatc aaaacgcttg ccgcccttta caaaaacagc cccaaaaaaa tgatgagccg
     3661 gttccttctc cagggcccat cgactcccgt acggtttctc aagagacgcg tctaatagcc
     3721 gaccgtccgg cgggcctttt ccagacattc gatgagatcc tgcatatgcc gctcttcaat
     3781 aaaaggcgac atgacaaatg acttgacggc caggaccggt tctccgcatg cggcggtgcg
     3841 gtaatgagcg gtcaccgaca acgcaaaacc gttttcctgc tccatctgac ggtgcagttc
     3901 atcaaatact ttccggttat agacattgct gcgcctcaga ctctcgacgg cctcggggct
     3961 gttcagctca tcgtaaaata cggcctttgc attcatccca tcaggatata cgcgaaacaa
     4021 ggttaccgga ccgtagttgt aatcattgac ctggcacatg gccggatgtt cgtcgagtcg
     4081 ttttctcagg acttcggcca tcgtgaccag gtgcccaaga agcccgcgat agccttcttt
     4141 gccgaacagc ttcatgttgg ccagagacgc gagcgcggcc ccgccggccc gcgaagtctc
     4201 cagtgtaaaa atgccgggat gatagttgcc gaattgaaac aggtaaggca tgaggcccgc
     4261 atcccggctg atcaggctca ggtcgtcttt gtttttgcaa agaaacatgc tggagacata
     4321 agggccgaaa cccgttttgt gaaaatcaat gccgacggaa tcggcgcggg acaaagcgct
     4381 gatgttgaca tgcgtatccc agaggcaccg cagggtccgg gcattgaaac ccagggtgtt
     4441 ggcatcgaag tcatagtcgt taaagaccga ccaggcccag ccgatgacgg catcggcgtg
     4501 gatatggggc aggtagggca attgatattc ggcggccagg ctgtcgcgca ggccggcgat
     4561 gtactggagg ttgtcgatgc cgaaagcatc ggtcgtgccc atggtggcga tgatggctgc
     4621 gatcttttct ccggagtcca gaatccttcg cagattggtt tcgagggcgg cgagatccat
     4681 ggaattatcg gcgtcggtgg gaacgctcac aaggttgtcc gttccgacgc ccagccagcc
     4741 taagacattg atctttgcat aatgcgccgc ggaggatgcc acaagtttca ggggctcatg
     4801 caggccgcgg ccgaacgttc cgggagatgc tttttcaacg ccgattttcg ctccgtatag
     4861 aaaggtgccg gttccgccga aagtgaaaaa tccggcggcg ctttgcggat catatccgac
     4921 cagggccgcg cacatggcgg ccgtttcaat ttcggcaagc cccacgcgat agctgtattc
     4981 gtccgagatg acgttcgggt tgtagatggc gccaaacaga ttgccgatga tggaaggcac
     5041 ggttgtcggc ggtacgacat tttcctgcgt gtggggatgg ctccagataa atcctccctg
     5101 catgtattcg gcgatgtcgg cgacggtttc agcgacaccc gacatgtttt ccggaatctg
     5161 tcgggaccgg gcctggctat agtcaatcgt ttcgcgccgg ccaagatagg gccggtccgt
     5221 tttcagtttg tcgatctgtt caagcccgcc cgcgacggcg gctacgaagt ggcggtcggc
     5281 gtccgcattg gagacgggcg atggaaaagc gccgcggatg agcggaagaa gttcggcata
     5341 ggttttgcct tgaaccggtt ttgttcccgg cgtcgcccga aaaccggagg agaaactttc
     5401 gagcaagccc gaaagttcca gcacctgacg gattgccgga ctggcctgaa tcagacgaaa
     5461 aggaagcaaa ttgcgcgttt gtcccgccgc ggagctcgca gccgccgtct tcatcaaaaa
     5521 cagcagggat cgaatacccg cgctggaaat atactgcagt tgagaaaaat cgagaacaac
     5581 ctgcttatcg cttttcttga ccagcggctc aagagtcgtt tcgaattctt tggcggaagc
     5641 aacatccagc cggccggagc attgcaccgt gatgcggctt gctctcgtct caacgataat
     5701 atccatggtt cgtcctttcc tgcaagataa gccttgagtg tttcaacaag ggaatcatca
     5761 catgcttaac ttttttaaca agtacgccat gttttttccc agggtcttca tgttctcgat
     5821 cccttcctgg tcattattga cttcacccgg cgcccatcca taaaccatgt tccagtagct
     5881 ggcgcccacc aaaaacatct gcatgtaatg aagaaaatga ttcatcgtat caaaggcatg
     5941 cgttgctccg cctcttctca ccgccactac tgaaccgccg gctttatgct taaaaattcc
     6001 gccattggct atagaaacca ttcctgcccg gtcgattaag gccttcattt cagcggttac
     6061 atccgtaaag tacgttggag atcccaaaat aattccatct gccgcggtca ttttgccaat
     6121 acattcgttc atgatgtcgt catcaaacac gcattgtttg ttttgatttt tcacgcattt
     6181 caaacagcct ttgcatcctc tgacaggctt ttgtgcaata tggataagct cggtctcaat
     6241 tccttcgttt tttaattctt gaaaaaccgt tttgatcagc aacgatgtat tgccattttt
     6301 atgcggactg ccattgattc ctaagacttt catcttactc ctcctaatcg tattattttc
     6361 tttcgaaatt ctaaccgcgg caggcatatt ttcccctcca tccgccaaat tgctttatga
     6421 tggatactgt ttcataatac agcatattgc aatatgttat ttcatggata atacgggcat
     6481 cagcggcgca ggctgttcag cgaaaataga accgtcccct ttattccgtc ggccttgacc
     6541 ggagggccgc ttttatctat aaggaagcct gcggaaacac cgcggcacaa aaagaataaa
     6601 cgcaaaagga gcctgattca tgtcactgca tctgaacaaa ctgcaatcct ttcggggccg
     6661 gaaaggccct ctgcttttga ttatcatgga cggcatcggc ctgggcaaag ccgatgaatc
     6721 caatgccgtt ttccgggcca acacacccaa ccttgacgaa ctgttcaagt caaagcttta
     6781 cgctcagctc agcgcccacg gcacggccgt cggacttccc tcggatgacg atatgggaaa
     6841 cagcgaagtc gggcacaacg cgatgggcgc gggccggatt tttgaccagg gcgcccgtct
     6901 ggtcaacgcc gcccttcaga ccggccggat atttcagaca gaagtctggg acagcatcgc
     6961 ccgccgtgct caagacggcg gcacggtcca ttttatcggc ctgctctccg acgggaacgt
     7021 ccattcgcac atcgatcagc tttacatcct gatcgaccaa tgcgcatcgc tgggcttcaa
     7081 aaaagtccgg ctgcacaccc tgctcgacgg ccgggacgtc ggcgaaaaaa cggcgctgaa
     7141 ttatctgggg cccaccgaag aaaaactgaa aaaaatctct gcggaaaaag gcctggatta
     7201 cgccgtcgcc tcgggcggcg ggcgaatgac gacaaccatg gaccgctaca attccgactg
     7261 gagcatcgtg aagcgcggct gggaagccca tgtttttgga aagggtcgga cgtttccttc
     7321 cgcttccgac gcggtccgga cgttttacgc ggaagacccg aaggcgacgg accagtacct
     7381 gcccgcgttt gtggtaaccg acggctcggg ccgggccgtc ggaaaaatcc aggacgggga
     7441 tgccgtcatt ctgttcaact tccggggcga ccgcgcgatc gaaatctccc gcgccttttc
     7501 cgaaaaggat ttttccgaat tcgaccgggg gcccctgccg aatgtctttt acgccggcat
     7561 gatggagtat gacggcgacg cccacattcc gcccaatttt ctggtgcagc ctccggctat
     7621 cgaccgcgtc atcggcgaat atctctgcgc ggaaaaggtc aaaacctttg ccgtgtcgga
     7681 aacgcagaaa tacggacatg tgacgtattt ctggaacggc aaccgctccg gctgcatcga
     7741 tgaagccctc gagaagtatg tggaaattcc ttccgaccgg attgagtttg accgcgcgcc
     7801 gaagatgaag gccaaagaaa taacggatat ggtgatcgac ttgctccaaa gcggcaacta
     7861 ccgcttcggc cggacgaact atcccaacgg cgacatggtc ggccacacgg gcgtgacgga
     7921 agcggccatt gtcgcggtgg aagccgtcga tcagtgcgtc gggaaactgc tggacgtcat
     7981 ccggaagctg ggagggatcg ccgtgatcac ggctgaccac ggcaacgcgg atcagatgtt
     8041 caccatcgac aaaaagggcg tcaaatccgt caagaccgcg cattccctga atcccgtc
//
LOCUS       DAOSIM010000076         8065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_8310_length_8065_cov_7.868290, whole genome shotgun sequence.
ACCESSION   DAOSIM010000076 DAOSIM010000000
VERSION     DAOSIM010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8310_length_8065_cov_7.868290"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1703
                     /gene="dnaX"
                     /locus_tag="PLR47_04780"
     CDS             <1..1703
                     /gene="dnaX"
                     /locus_tag="PLR47_04780"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOG12289.1"
                     /translation="EYQVLARKFRPQTFEEVVGQEPVVRTLVNAIGQGRIGHAYLFAG
                     PRGVGKTSVARILAKSLNCEHGPTAVPCGKCPNCTDITGGTSMDVREIDGASNRGIDE
                     IRELRENVKFAPAASRYKIYIIDEVHMLTREAFNALLKTLEEPPGHVAFVFATTENHK
                     VPATILSRCQCYEFRRISLSQIAANLGKVAAKENIVISPAALSWIAEAGDGSMRDAQS
                     IFDQIISYAGQTISDEDVEDLLGLSDRRYLHRLTESVLARNASECLIILEEAYLAGVD
                     MKHFYTMLLKHFRNLLLIKIAGDGSSSFDIAPEQVEKLKTQTSKVSRETLQRYLDILL
                     DEADSLRRSQEARMKIEAVLVRLAYLEPVLPVGEILATLEALEERMTAGSPAARGGAD
                     AGPAAAKQPSEIRQAVIAEGEPVIPSRQATAPAEPRSVEKPGPNDDLKQFINKENSLL
                     GAKIAKAEVLGLENGCLTLGFPQGYVFLDNLLEKQQKEELERIAGAYYSRKVSLKIIT
                     IEAPKANGNGGGRGARPNNLGDLKREAVNSPLFRKVLAEFDGAELVEIKPITDKNHRG
                     "
     gene            1708..2025
                     /locus_tag="PLR47_04785"
     CDS             1708..2025
                     /locus_tag="PLR47_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOG12290.1"
                     /translation="MHNIGNIVKQAKKMQEKMGRLQQELETRTVEAQSGGGMVRVWVN
                     GKFEIVSLTIEKDVVNPEDVEMLQDLIAAAVNEGIRKSQEMASQEMAKITGGLAIPGL
                     GGM"
     gene            2034..2633
                     /gene="recR"
                     /locus_tag="PLR47_04790"
     CDS             2034..2633
                     /gene="recR"
                     /locus_tag="PLR47_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOG12291.1"
                     /translation="MKSYAQPIKRLIAELAKLPGIGEKTAARLANYILRATPEDVEKL
                     AESLVEVKRKIRLCRICLNPTEEEVCHICGNPARDQHVICVVEEPDALAAIEESGVFH
                     GLYHVLHGALAPLDGIGPENLRLGELSRRISEGRIQELILATNPNVHGEATALLITRM
                     FKDADIRITRIAMGVPMGGDLKYVDKMTISKSMEFRRGM"
     gene            2773..3645
                     /locus_tag="PLR47_04795"
     CDS             2773..3645
                     /locus_tag="PLR47_04795"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOG12292.1"
                     /translation="MDTEITTKTKLRRINTRRVTAAVKKLFIEANTVLGADVVTALRK
                     GLKREESPVGRQVLEKILENAGIAGREAMPICQDTGLAVLFVEMGQDVRVVGGNLREA
                     IEEGVRKAYQDGYLRKSVCDPLTRANTKDNTPAIIHMDVVPGNQLKITVMPKGGGSEN
                     MSAAKMLTPSAGIEGIKKFILEIVEKAGANPCPPIIVGVGIGGSLEQACILAKKALLR
                     PVGKKNNKDKRLAGMEGELLKKINATGIGPAGLGGRVTALAVHAEMLPCHIASLPVAV
                     NIQCHVARHKETTL"
     gene            3862..4422
                     /locus_tag="PLR47_04800"
     CDS             3862..4422
                     /locus_tag="PLR47_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HOG12293.1"
                     /translation="MATTQKIHTPLTHEVIAFLRAGDMVLLSGDLYTARDAAHRRMLD
                     AVQKGLPLPVDLKTAAIFYAAPSPTPPGRVIGSIGPTTSCRMDAFTPLLMEQGLRVMI
                     GKGSRSREVAAAVKKYGGVYFGAIGGIAALTAKCVKKMELVAYEDLGPEAIRILTVSD
                     LPLVVANDSRGNDLYKTEQNKWRIFP"
     gene            4527..5075
                     /locus_tag="PLR47_04805"
     CDS             4527..5075
                     /locus_tag="PLR47_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOG12294.1"
                     /translation="MIEVRWHGRGGQGAVTSVELLAVSAIAAGKFAQGFPSFGPERRG
                     APVAAFTRVADKKINVRSGIYEPDVVLVLDSSLIGLVNVTEGLKPDGKLIVNTPKSPE
                     EIRTALDYRGTLALVDGTGIARKEMGVPIANTTMIGALLKVTGIMKLDAMKEAIEHRF
                     GRIAQKNLNAMKRAYDEITIYN"
     gene            5089..5367
                     /locus_tag="PLR47_04810"
     CDS             5089..5367
                     /locus_tag="PLR47_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG12295.1"
                     /translation="MALKTWKELPIGCVVSEPGSAGEYHTGSWRSQRPVWDDAKCIKC
                     GICYVFCPEGCVQQGEDGFFKANLDYCKGCGICSHECWPRAIKMVEEG"
     gene            5369..6544
                     /locus_tag="PLR47_04815"
     CDS             5369..6544
                     /locus_tag="PLR47_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOG12296.1"
                     /translation="MGKRIGMEVAVAVAEAVGLCNIDMAAVYPITPQSHIAEHLSDLV
                     ANGQIDAEFVTVESEHSAMSAVIGASGAGARSYTATSSQGLMYMHELLPIASAMRLPI
                     TMAIANRAVSGPLNILNDHTDIMPQRDSGWISVFVENGQEAIDMSIMAFKIAEHKDVM
                     LPVNVNIDGFQLTHMVEPFEMPSQEEVDAFLPPFVPHATLHPAKPVTMGAFAMSDYFT
                     EIMKAKDEALLNSKKVILDVWDEWAKMFGRSYKPVETYKADDADVLMLTMGSMGETAQ
                     MAIDQLRGRGVKAGLVKLRLWRPFPHSEIKAVLKNVKKLIVTDRAISFGGPGGPVFSE
                     IKSALYAEASRPLIYNYIFGLGGRDVAVGEFVGMVDKVLADTKNKAADTYEFWGVRE"
     gene            6544..7497
                     /gene="porB"
                     /locus_tag="PLR47_04820"
     CDS             6544..7497
                     /gene="porB"
                     /locus_tag="PLR47_04820"
                     /EC_number="1.2.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit PorB"
                     /protein_id="HOG12297.1"
                     /translation="MNIVENFDLFAPRLINKQELLSSGHRACSGCAEVLAVRLMCKAL
                     GENTVIASATGCMEIVSSMFPTTAWQVPWIHVAFENAASVASGIEAGLKALRKKGRID
                     DRYIKVVGMGGDGATMDIGFQALSGAMERGHDMIFVCFDNEAYMNTGIQRSSGTPMGA
                     STTTAPAGKVSMGQKTWKKNMPEIMVAHEIPYVATVCPSYPLDFIRKIEKAKSIKGPS
                     YIHCFSMCPTGWRSPSHTAVSIGRLAVETGVFPLFEVEHGHYRLSPDMPKKLKPIKDY
                     FKPQGRFRHLTPDEIEVIQERVTVEFRKLAEKAKFLKTGNE"
     gene            7534..8019
                     /gene="nuoE"
                     /locus_tag="PLR47_04825"
     CDS             7534..8019
                     /gene="nuoE"
                     /locus_tag="PLR47_04825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOG12298.1"
                     /translation="MTKPIGVAAKTRQIVKKHGGGKSAMIAVLQDIQETFNYLPKEAL
                     KAAARAMNVPMSRVYEAATFYTAFSLKPRGKHVVKICKGTACHVRGAAILQDRFETTL
                     GIKPGGTTKDGKFSLETVNCVGACALGPVVVINADYHGQVTISKVDKIIKKINEEGAK
                     S"
BASE COUNT         1963 a         2134 c         2365 g         1603 t
ORIGIN      
        1 tggaatacca ggttttagcc cgaaaatttc gcccgcagac gtttgaggaa gtcgtggggc
       61 aggagccggt ggtccggacg ctggtgaatg ccatcggcca gggaagaatc gggcacgcct
      121 atctttttgc cgggccccgc ggcgtcggca aaacctccgt ggcccgcatt ctggccaagt
      181 cgctcaactg cgaacacggg ccgacggccg ttccctgcgg caagtgtccg aactgcacgg
      241 acattaccgg cggcacctcc atggacgtgc gggaaatcga cggcgcctcc aaccgcggca
      301 tcgacgagat tcgcgagctt cgtgaaaacg tcaaattcgc gcccgccgcg tccaggtaca
      361 aaatctacat catcgatgaa gtccacatgc tgacgcgcga agccttcaac gcgcttctga
      421 aaacgctgga ggaaccgccg gggcacgtcg cttttgtttt tgcgacaacg gaaaaccaca
      481 aggtgccggc caccatcctg tcgcgctgcc agtgctatga attccggcgc atctcgcttt
      541 cgcagatcgc ggccaatctg ggcaaggttg ccgcgaagga aaacatcgtc atcagcccgg
      601 cggctctttc ctggatcgcg gaggcgggcg acggctccat gcgcgacgcc cagagtattt
      661 ttgatcagat catttcctac gcaggccaga cgatttcgga cgaggatgtg gaagaccttc
      721 tgggactttc cgaccgccgc tatctgcacc ggttgaccga gtccgtattg gcgcgcaacg
      781 cttcggaatg cttgattatt ctggaagaag cctacctggc cggcgtggat atgaagcact
      841 tttatacgat gcttttgaag catttccgga atttgcttct gatcaaaatc gccggggacg
      901 gaagttcctc ttttgacatt gcgccggagc aggtggaaaa gctgaaaacc cagacgtcaa
      961 aggtgtcccg tgaaacgctg cagcgttatc tggacattct gctggacgag gccgattcat
     1021 tgcgccgcag ccaggaggcg agaatgaaaa tcgaggccgt tctcgtccgg ctggcctatc
     1081 tggaaccggt tttgcccgtc ggagaaattc tggcgacgct ggaagcgctg gaagagcgga
     1141 tgacggccgg ttcaccggcc gcgcggggcg gagcggatgc cggacctgcc gcggcgaaac
     1201 agccttctga aatccggcag gcggtcatcg ccgaaggcga gccagtgatt ccttcgcggc
     1261 aggccacggc gccggcggag cctcgaagcg tcgagaaacc gggtccgaac gatgatctga
     1321 agcagtttat caacaaagag aattcactgc tgggggcaaa aatcgccaag gctgaagttc
     1381 tgggattgga aaacggctgc ctgacgctgg gctttcccca aggctatgtt tttctcgata
     1441 atcttttgga aaaacagcag aaagaagagc tggaacgaat cgcgggcgct tattattcac
     1501 ggaaagtatc cttgaagatt atcaccatcg aagcgccaaa ggccaacggc aacggcggcg
     1561 gacgaggcgc caggccgaac aacctcggcg acctcaagcg cgaagccgtg aactcgccgc
     1621 ttttccggaa agtgctcgcc gaatttgacg gcgcggaact ggtcgaaatc aaaccgatta
     1681 cggacaaaaa ccacaggggg tagcgaagtg cacaatatcg gcaatatcgt caaacaggca
     1741 aaaaaaatgc aggaaaagat gggccggctt cagcaggagc ttgaaacgag aaccgtggag
     1801 gcgcagtcgg gcggaggcat ggtgcgcgtt tgggtcaacg gaaaatttga aatcgtgtcg
     1861 ctcacgatcg aaaaagacgt ggtcaatccc gaagacgtgg aaatgctgca ggatttgatt
     1921 gcggcggccg tgaatgaagg catccgcaag tcgcaggaaa tggcctcgca ggaaatggcc
     1981 aagatcacgg gcggcctggc gatcccgggc ctgggcggca tgtaaggcgt cccatgaaat
     2041 cctacgcgca acccattaaa cgcctgattg cggaactggc caaacttccc ggcatcgggg
     2101 aaaaaacggc ggcgcgcctg gcgaattata tattgcgggc cacccccgag gatgtggaaa
     2161 aactggcgga aagcctggtt gaagtcaaac gcaaaatccg tctttgccgg atatgcctaa
     2221 atccgaccga agaggaggtc tgtcacatct gcggcaatcc ggcgcgcgat cagcacgtca
     2281 tttgcgttgt cgaggaaccg gatgccctgg ccgccattga agaaagcggc gtttttcacg
     2341 gcctgtatca cgttttgcac ggggcgctgg cgccgctgga cggcatcggg ccggaaaatc
     2401 ttcgcctggg cgagctttcc aggcgcatca gcgaaggccg cattcaggaa ttgattctgg
     2461 ccaccaatcc caatgtgcac ggcgaagcca cggcgctgct gattacgcga atgttcaaag
     2521 atgcggatat ccggatcacg cgcatcgcca tgggcgttcc catgggcggc gatctgaaat
     2581 acgtcgataa gatgaccatc tccaaatcca tggaatttcg gagaggcatg tagcatgacg
     2641 ctttaaaaca gccatatgct gcgttgcgct tcatctttcg tcactgcggc ctacgcaaaa
     2701 gtaggcctca ttcctcaata ttcgcgcgcc ttgcctctga ctgttttaaa gcgtgatgga
     2761 gaaaacagtt gtatggatac ggaaattacg accaaaacaa aattacggcg gatcaatacc
     2821 aggcgcgtaa cggcggcggt taaaaaactg tttatcgagg ccaatacggt tctgggggcg
     2881 gatgtggtta ccgctctgcg caagggatta aagcgcgagg aatcgcccgt cggccggcag
     2941 gtgcttgaga aaattcttga aaacgccggg atcgccgggc gcgaggcgat gccgatttgc
     3001 caggatacgg gtctggccgt tttgtttgtc gaaatgggcc aggatgtccg cgtcgtgggc
     3061 ggcaatttac gggaagcgat tgaagaaggc gttagaaagg cctatcagga cggatacttg
     3121 cgaaaatcgg tctgcgatcc gctaacccgt gccaacacaa aagacaacac tccggccatc
     3181 atccacatgg acgtcgtacc gggcaatcaa ttaaaaatca ccgtcatgcc caaaggcggc
     3241 ggcagcgaaa acatgagcgc ggccaaaatg ctgacgcctt cggcgggaat cgaaggcatt
     3301 aaaaaattta ttctggaaat cgtggaaaaa gccggcgcca atccctgtcc gccgattatc
     3361 gtgggcgtgg gtatcggcgg ctcgcttgaa caggcttgca tcctcgcgaa gaaagcgctt
     3421 ttgcgtcccg tgggcaaaaa aaataacaag gacaaacgcc tggcgggcat ggaaggcgag
     3481 cttctcaaaa aaatcaatgc gacgggaata ggcccggcgg gtctgggtgg ccgtgttact
     3541 gcgctggccg ttcacgcgga gatgctgccc tgtcacattg cctcgctgcc ggtggcggtg
     3601 aatatacaat gtcatgtcgc ccgacacaag gagacgactc tgtgaaatgc cccatatgct
     3661 gcgttgcgct tcgaccttga ggcgttgcgg cgtatgaaaa atacgcctca cttccaaggc
     3721 cttgcgcgcc ttgactctga tgcatttgac aaagtcgtca taacttgtca gcgcacacgt
     3781 gggggagggt gataaacggt ttacccctcc cctcaatccc ctcccacaag gggaggggcg
     3841 gtcaaaggac ggtaatggta aatggcaaca acacaaaaaa ttcatacgcc gttgacccat
     3901 gaagttattg cttttctacg agccggcgac atggttctgc tcagcggaga tctttacacg
     3961 gcccgcgacg cggcgcaccg tcgcatgttg gatgccgtcc aaaaaggtct gccgctgccg
     4021 gtggatctga agacggccgc cattttttac gccgctccct cgccaacgcc acccggaagg
     4081 gtcatcggtt ccatcgggcc gacgaccagc tgccggatgg atgcctttac gcctttgctg
     4141 atggaacagg ggttgagggt catgatcggc aaaggcagcc gctctcggga agtggcggcc
     4201 gccgtcaaaa aatacggcgg ggtttatttc ggcgccatcg gcggcatcgc cgctctcacg
     4261 gcaaaatgcg tcaaaaaaat ggaattggtc gcctatgaag acctgggccc ggaagccatc
     4321 cgcattctca ctgtctcgga cctgccgctt gtcgtcgcaa acgattcgcg gggaaacgat
     4381 ttgtacaaaa ccgagcagaa caagtggcgc atcttcccgt aatagcggtt catttgaata
     4441 atcaagaaaa gatattgact caaaaaacca tctatattaa ggaaaacggt cattttgtaa
     4501 acattcaatg aaggagactg cgatacatga ttgaagtaag atggcatgga cggggcggtc
     4561 agggagcggt cacgtcggtg gagttgctgg ccgtgtcggc catcgccgcc ggaaagttcg
     4621 ctcagggatt tcccagtttc ggtccggaaa gaagaggcgc gcctgtcgcg gcatttaccc
     4681 gtgttgccga taaaaaaatc aatgtccgct cgggaatcta tgaaccggac gtggtgctgg
     4741 tgctggattc cagtttgatc ggactggtca acgtgaccga gggcctgaag ccggacggca
     4801 aattgatcgt caatacgccg aaatcgccgg aagagattcg cacggcgctc gattacagag
     4861 gaacgctggc gctggtggac ggcacgggca tcgcccgcaa ggaaatgggg gtgccgatcg
     4921 ccaacaccac gatgatcggc gcgcttctca aggtaaccgg cattatgaag ctggacgcga
     4981 tgaaggaagc catagaacac cgtttcggca gaatcgccca gaaaaatctc aacgcgatga
     5041 aacgggctta cgacgaaata accatataca attaagaggt agatctcaat ggcattgaaa
     5101 acatggaaag aacttcccat tggctgcgtg gtttccgagc cgggaagcgc cggagaatat
     5161 catacgggaa gctggcggtc tcaacgaccg gtatgggatg acgcgaaatg catcaagtgc
     5221 ggaatctgct acgtcttttg ccccgaggga tgcgttcagc agggcgaaga cggttttttc
     5281 aaggcgaatc tggattattg caagggatgc ggcatctgtt cgcacgaatg ctggccccgc
     5341 gcgatcaaga tggtggagga gggataacat gggcaagcgc atcggaatgg aagtggcagt
     5401 tgccgtcgcg gaagctgtcg ggctctgcaa tatcgacatg gcagccgttt atcccatcac
     5461 tccccagtcg cacatcgcgg agcatctttc cgatctggtc gccaacggcc agattgacgc
     5521 cgaattcgtg acggtggaat ccgaacattc ggccatgagc gcggtcatcg gcgcctccgg
     5581 cgcaggcgcc cgttcttaca cggcaaccag ttcgcagggg ttgatgtata tgcatgaatt
     5641 gctgccgatt gcttcggcca tgcgtctgcc gattaccatg gcaatcgcca accgggcggt
     5701 gtccggtccg ctcaacattt taaacgacca cacagacatc atgccccagc gtgattccgg
     5761 atggatctcc gtgttcgtgg aaaacggcca ggaggcgatc gacatgtcca ttatggcttt
     5821 caaaatcgcc gagcacaaag acgttatgct gccggtcaat gtcaacattg acggttttca
     5881 gttgacgcac atggtggaac cgtttgaaat gccctcacag gaagaagtcg atgcgtttct
     5941 gccgcccttt gtgccgcacg cgaccttgca tccggccaaa cccgtgacga tgggcgcctt
     6001 cgccatgtcc gactatttta cggaaatcat gaaagccaag gatgaagccc tcctgaattc
     6061 caaaaaggtc attctggacg tctgggacga gtgggccaaa atgttcggcc gctcctacaa
     6121 gccggtggaa acctacaagg ccgatgacgc ggatgtgctg atgctgacga tggggtccat
     6181 gggcgaaacc gcgcagatgg cgatcgacca gcttcggggc aggggagtca aggcaggtct
     6241 ggtaaaattg agactctggc gtcctttccc tcactcggaa atcaaagccg ttttaaaaaa
     6301 cgtaaagaaa ttgattgtga ccgaccgcgc catatctttt ggcggccccg gcggcccggt
     6361 gttttcggaa atcaaatcag cgctttatgc tgaagccagc cgccctttga tttacaacta
     6421 cattttcgga ctgggcggac gcgacgtggc cgtgggagag tttgtcggca tggtggacaa
     6481 ggttctggcg gatacaaaaa ataaagccgc ggacacctac gaattctggg gggtaagaga
     6541 ataatgaata ttgtagaaaa ttttgattta tttgccccaa ggctgatcaa taaacaggaa
     6601 ttgctttcct ccgggcatcg cgcctgctcc ggctgcgccg aggtgctggc cgtccgattg
     6661 atgtgcaagg ccctgggtga aaataccgtc atcgcttccg cgacggggtg catggaaatc
     6721 gtttccagca tgtttcccac cacggcgtgg caggtgccct ggattcacgt ggcgtttgaa
     6781 aacgcggcgt ccgttgcttc cggtattgaa gccggcctca aagcgctgcg caaaaaaggc
     6841 aggatcgacg accgctacat taaagtcgtc ggcatgggcg gcgacggcgc caccatggac
     6901 atcggctttc aggcgctttc cggcgcgatg gagcgcggcc atgatatgat ctttgtctgc
     6961 tttgacaacg aagcctacat gaacacgggc attcagcgct ccagcggcac gccgatgggc
     7021 gcctccacca ccaccgcgcc cgcgggcaag gtcagcatgg gccagaaaac ctggaagaaa
     7081 aacatgccgg aaatcatggt cgcccacgag attccctatg tggccaccgt ctgtcccagc
     7141 tatccgctgg atttcatccg caagattgaa aaggccaaaa gcatcaaagg gccgtcttat
     7201 attcactgtt tttccatgtg tccgaccggc tggcgttcac cgtcgcacac ggcagtatcc
     7261 ataggtcgcc ttgccgtgga aaccggcgtg ttccccttgt ttgaagtgga gcacggccat
     7321 tatcgtctgt ctccggatat gccgaagaaa ctcaagccga tcaaggatta cttcaagccg
     7381 caggggcgct tccgtcatct gactccggat gaaattgaag tgatccagga gcgggtgacg
     7441 gtcgaattca ggaaacttgc ggagaaagcc aaatttctca aaaccggcaa tgaataaaag
     7501 agaaattgaa agaataccga ggtgagagaa aaaatgacga aaccgatagg tgttgcagca
     7561 aaaaccagac agattgtcaa aaaacacggg ggcggcaaaa gcgccatgat cgccgttttg
     7621 caggatattc aggagacctt caactacctt ccgaaggaag ccctgaaagc ggcggccagg
     7681 gcgatgaacg tgccgatgag ccgcgtctat gaggccgcga cgttttatac ggccttcagt
     7741 ttgaagccgc gcggaaagca tgtcgtcaag atctgcaagg ggaccgcctg ccacgtccgc
     7801 ggcgcggcca tccttcagga ccggttcgaa accacgctgg gcatcaagcc ggggggaacc
     7861 acgaaggacg gaaaattttc tctggaaacc gtcaactgcg tcggcgcctg cgcgctcggg
     7921 cccgttgtgg tgatcaatgc cgactaccac ggtcaggtca ccatcagcaa agtggataaa
     7981 atcatcaaaa agatcaatga agagggggcg aagtcatgaa cgaaaatccg gatactcaag
     8041 cccgccagat catggataat ttgac
//
LOCUS       DAOSIM010000077         8030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_8355_length_8030_cov_7.356489, whole genome shotgun sequence.
ACCESSION   DAOSIM010000077 DAOSIM010000000
VERSION     DAOSIM010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8030
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8355_length_8030_cov_7.356489"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2073)
                     /locus_tag="PLR47_04830"
     CDS             complement(<1..2073)
                     /locus_tag="PLR47_04830"
                     /inference="COORDINATES: protein motif:HMM:NF018357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HOG12299.1"
                     /translation="MNYKKKLIEVALPLDAINKASAREKSIRHGHPSTLHLWWARRPL
                     AAARAVIFSQMVDDPSAHPDLFPTEKAQEKERKRLFKIIEDLVLWENTTNEKVLQQAR
                     DEIWQSWRYTCAENAGHPRAKELFDRHKLPAFHDPFAGGGALPLEAQRLGLEAYASDL
                     NPVAVLINKAMIEIPPKFAGKPPVNPEWQQLPDGQKALRTWKGAQGLAEDVRYYGKWM
                     RDEAEKRIGHLYPKVEITTEMVKERSDLKPYLGKKLTVIAWLWARTVKSPNPAFANVD
                     VPLASTFMLSAKAGKEAYVEPVIENGGYRFTVKVGKPKDAEAAKNGTKLSRGAHFQCI
                     MSGTPIAADYIKAEGKAGRMGVQMMAIVAAGDRGRIYLPPTAAMESVARQTNPAWQPE
                     GTLVEDARAFTPTLYGLAKWYDLFTARQLVTLTVLSNLVQEACAMIKRDALAAGLLDD
                     SKFLDPGIGIISYADAVGVYLSFALSKQADLGNSLCRWEPIAQCPRQLFGRQAIPMVW
                     DFAEGNPLGDSSGAWIVLIDGITKAFAKAFEFVSSKVIGSALQADASRQDASKAKVVS
                     TDPPYYDNVPYADLSDFFYVWLRRSLQPVFPDLFATLAVPKTEELVAFGYRHGGKVDA
                     EAFFLNGMKQAMQRLAEQSHPAFPVTIYYAFKQVENDGADGNASTGWETFLEAVICAG
                     FSTTGTWPM"
     gene            complement(2220..2453)
                     /locus_tag="PLR47_04835"
     CDS             complement(2220..2453)
                     /locus_tag="PLR47_04835"
                     /inference="COORDINATES: protein motif:HMM:NF027067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HOG12300.1"
                     /translation="MKTKNCPICGAGKLKKKVETESFEYKGQTKVIPNYITYACSECG
                     EAIVDSTTLKESGKMLEDFKLKMEVAFDDEIFR"
     gene            complement(2460..3683)
                     /locus_tag="PLR47_04840"
     CDS             complement(2460..3683)
                     /locus_tag="PLR47_04840"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG12301.1"
                     /translation="MNENTFPRHMMAALGQRLRVMPAVVVTGARQTGKSTLVKVLTLE
                     KRSYFSLDDMDVFELARQNPEALVGGAAKVTLDEVQREPDLLLAVKRAIDSDRQPGRF
                     LLTGSANLLLMQGVSESLAGRASYLTLWPMTRREQLGLGRCGLWEELVAANDAEWPDL
                     LSVDTSGPEDWLAFARRGGFPTPAVHMTRAEDRMVWFDGYVRTYLERDLQVLSSIAAL
                     PDFRRLMRAACFRLGQLVNQTEMARDLSLKQPTIHRYLNLLETSYLLIRLPAYSVNRT
                     KRLLKSPKLYWGDVGLAMHLSGMAEPTGAYLENIVLLDLLVWQCGRLQNTEILYWRTT
                     TGEEVDLVIETDGKVLPIEIKSTNRPRIKDAMNLLAFQNEYGADARAGLLLHTGTVIE
                     WLTPRVLAVPWWKVI"
     gene            complement(3723..7304)
                     /locus_tag="PLR47_04845"
     CDS             complement(3723..7304)
                     /locus_tag="PLR47_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOG12302.1"
                     /translation="MIRLEDLQPNAAVCGLLPDCLITVVSVQWFGSEALELTYKTPTG
                     KVANELLYRYDEPRLDLVEQGRPWSFDGDGALFRLVSEAHRIRLAHLFDPVLAVHTSI
                     VDPLPHQITAVYEAMLPRQPLRFLLADDPGAGKTIMAGLLIKELVARGDLQRCLIVCP
                     GSLAEQWQDELYHRFHLPFEILTNDKLQAARTGNWFLETNLVIARLDKLSRNEDVQQK
                     LQTPDCRWDLVVCDEAHKMSATIFGTETKYTKRYRLGQLLSTLTRHFLLMTATPHNGK
                     EADFQLFMALLDGDRFEGRFRDGVHVADVSDLMRRMVKESLLKFDGTPLFPERIAYTV
                     PYKLSEAEAKLYKAVTEYVREEFNRAEALQNDKRTGTVGFALTILQRRLASSPEAIYQ
                     SLRRRRERLESRLREMEVLQRGGQAAPILAADLLALDAEDVEDLEDAPDNEVKAAEEE
                     ILDQATAARTITELKAEIETLKGLESLALGVRRSGTDTKWRELSTLLGEIFTTAAISN
                     RLAEPITPFGAGAIPRHTPSLQQKLVLFTEHRDTLNYLENRITTLLGRKEAVVMIHGG
                     MGREERMKEQESFKHDPKVQVLLATDAAGEGINLQRAHLMVNYDLPWNPNRIEQRFGR
                     IHRIGQTEVCHLWNLVAEETREGDVYRKLLDKLEQARQSLGGQVFDVLGKLQFEGKLL
                     RDLLIEAVRYGERPEVKAYLTTVIDHALDRDQLRDLLEDHALAHDAMDASRIQHIRED
                     MERAEARRLQPHYIESFFLEAFRYLGGSARQREPRRYEVTHVPAPVRNRDRLIGIGEP
                     VLPRYERIAFEKPLVAPQGLPLAAFVCPGHPLLDSVIDLTLERHRDLLKRGAVLVDER
                     DTGIQPHVMFYLEHTIQDASLTRSGERRVVSRRMLYIELDADGATHHVNYAPYLDYRP
                     LAKGEPTVEAILNRPECTWITRELEKKAQGHAIVNVVPEHLAEVRGPKLKLIAKTEAA
                     VKERLTKEITYWDHRAEQLKLLEQAGRTGNRLNSGEARKRADLLQGRLQKRLEDLKLE
                     AQISPLPPVVLGGMLIVPKGLLTAMTGQGAAISTAPADTQASAARARAIIMEVERNLG
                     FEPTDRELEKLGYDIESCIPGTGKLRFIEVKGRVSGAPTVTVTRNEILYSLNKPEDFI
                     LAIVEFLDGDSYRVHYLRQPFQREPDFGVTSVNYDFAELLAKAKEPS"
BASE COUNT         1766 a         2213 c         2199 g         1852 t
ORIGIN      
        1 cattggccat gtcccggtgg tagagaaacc cgcacaaatc accgcctcta ggaaagtctc
       61 ccaacctgtg ctcgcgtttc catcagcgcc atcgttttcg acttgtttga aagcataata
      121 gatggtcacc ggaaatgccg gatgcgactg ctcggcaagg cgctgcatcg cttgcttcat
      181 accattgagg aaaaacgcct ctgcatccac cttaccaccg tgacgataac cgaaggcaac
      241 taattcttca gttttaggca cagcaagcgt agcaaagaga tctgggaaga caggctgtaa
      301 cgatctgcgc aaccagacgt agaagaagtc agagagatcg gcatagggta cgttgtcgta
      361 gtacggcgga tcggtggaaa cgacttttgc tttagacgca tcctgccgac tagcatccgc
      421 ctgtagggca gaccctatca ctttcgacga aacaaactcg aaggctttag cgaacgcctt
      481 cgtaatccca tcaatcaata cgatccaagc accagagctg tcgccgagcg gattcccttc
      541 cgcaaaatcc cagaccattg ggatggcttg ccgtccaaat agctgacgag gacactgtgc
      601 gatgggttcc cacctgcaga gactatttcc aaggtcggcc tgtttgctca gggcgaaaga
      661 tagataaaca cctacggcat cagcgtagga tatgatacca ataccaggat cgaggaattt
      721 gctatcatcc aacaggcccg cggcaagtgc gtcgcgcttt atcatcgcgc acgcctcctg
      781 taccaagttg gataagaccg tcagcgtcac gagttgccgg gcagtgaaaa ggtcgtacca
      841 tttcgccagc ccatacaggg taggcgtaaa agcccgtgcg tcttcgacca aagtgccttc
      901 tggctgccac gccggattcg tctgacgcgc tacagactcc attgccgcgg ttggcgggag
      961 gtaaatccgc ccgcgatcac ccgcagcaac gattgccatc atctgcacac ccatgcgccc
     1021 cgccttgccc tcagctttta tgtaatcagc ggcaatcgga gtcccagaca ttatgcattg
     1081 gaaatgcgcg ccgcgtgaaa gctttgtacc attcttcgca gcctcggcat ccttcggctt
     1141 gcccaccttc accgtgaagc ggtaaccacc attctcgatc accggttcga cataggcttc
     1201 cttgccagcc ttggctgaaa gcatgaaagt ggaggcgagc ggcacatcca cattggcaaa
     1261 ggctgggttc gggctcttta ccgttcgtgc ccatagccac gcaatgacgg tgagcttctt
     1321 tcccaaatag ggtttgagat ccgagcgttc tttcaccatc tctgtcgtaa tctcgacctt
     1381 ggggtagaga tggccgatgc gcttttcagc ctcgtcgcgc atccattttc cgtaataacg
     1441 cacgtcctcg gcaaggccct gtgcgccttt ccatgtcctc agggcttttt gcccatccgg
     1501 caactgctgc cattccggat tgaccggcgg cttgccggca aacttgggcg gaatttcgat
     1561 catcgccttg ttgatcagga ccgccacagg atttaggtca ctggcatacg cttccaaacc
     1621 gagccgttgc gcctccagcg gcagtgcacc accaccggca aaaggatcat gaaaagcagg
     1681 tagcttgtgg cggtcaaaaa gttccttggc gcgcgggtga ccggcatttt cagcacaagt
     1741 atagcgccag ctttgccaga tttcgtctct ggcttgctgg agaactttct cattggtcgt
     1801 gttttcccag agcaccaggt cctcgataat cttgaagaga cgttttctct ctttttcctg
     1861 tgccttttct gtggggaaaa gatcaggatg cgccgaaggg tcgtccacca tctgcgaaaa
     1921 gatcaccgcc cgcgccgccg ccaagggccg ccgcgcccac cagaggtgca gggtgctcgg
     1981 atggccgtgg cgaatggact tctcccgggc cgacgccttg ttgatggcat ccagcggcag
     2041 ggcaacttcg atgagtttct tcttgtaatt catgatcttt ccttatgact ccggtcgaaa
     2101 atctgacgat tagcgtcatt catcggacga ttatccgacg attaaggcag aatatcggtc
     2161 gattccgagg aaactcggaa ggcggtgaaa tcggaagatt acggcaaaac atcgaaagat
     2221 tatcggaaga tttcatcatc aaaagccact tccattttga gcttaaaatc ttccagcatt
     2281 ttgccggatt ctttcaaggt cgtactgtca acgatagcct ctccgcattc ggaacatgca
     2341 taggtaatgt aattcggaat tacctttgtt tgccctttat attcaaagga ctcggtttca
     2401 actttctttt tcagcttgcc ggctccgcag ataggacaat tttttgtttt catttttttt
     2461 cagatcacct tccaccaggg caccgccaaa accctgggcg tcaaccactc aattaccgtg
     2521 ccggtgtgca gcagtaaccc ggcacgtgcg tcagcaccat actcattttg aaaggcaagc
     2581 aggttcatgg catccttgat ccgcgggcgg tttgtggatt tgatctcgat gggaagcact
     2641 ttcccgtccg tctcgatcac cagatccact tcttcacctg tcgtcgtccg ccaatagaga
     2701 atctccgtat tctggagcct tccacactgc cagacaagaa ggtcaagcaa cacaatgttc
     2761 tcgagatagg cgcccgttgg ttcggccatt ccagagaggt gcatcgccag tccgacgtcg
     2821 ccccagtaca gcttggggga tttgaggaga cgtttggtac ggttcaccga ataggccggc
     2881 aagcgaatca aaaggtacga agtttcaagc aggttcaggt agcggtgtat cgtgggctgc
     2941 tttaatgata gatcgcgggc catttccgtc tggtttacga gctgcccgag acgaaaacag
     3001 gcggcgcgca tcagccggcg aaaatcaggc agggcggcta tggaggaaag cacctggagg
     3061 tcccgctcca agtaggtccg gacatagccg tcgaaccaga ccatccggtc ctccgcacgg
     3121 gtcatgtgga cagcgggcgt tggaaatccg ccgcgacggg caaatgccag ccagtcttcc
     3181 ggacccgatg tgtccacact gagtagatcg ggccattcgg cgtcattcgc tgcgaccagt
     3241 tcttcccaaa gtccgcaacg gccgaggccc aattgctccc gccgggtcat cggccaaagg
     3301 gtgaggtagc tggcgcgacc ggcgagagat tcggatactc cctgcatcag gagcaggttg
     3361 gccgatccgg tcagaagaaa tcggcccggt tggcggtctg aatcgatggc ccgtttaaca
     3421 gcgaggagca aatcgggttc ccgttgcacc tcatcgagag tcaccttcgc cgcacccccc
     3481 accagggctt caggattttg cctggcgagt tcgaaaacat ccatgtcatc gagggaaaag
     3541 taggaacgct tttccagggt aagcaccttt accagagtgc tcttgcccgt ctgacgggcg
     3601 ccggtaacca caacagccgg catcacacgt aaccgttggc ccaatgcggc catcatgtgt
     3661 cggggaaatg tattttcatt catggcgtga attatatcca ttcatggacg attttgcaag
     3721 gattatgagg gttctttcgc tttggccagc aactcggcaa agtcgtagtt gacgcttgtc
     3781 acgccgaaat ccggctcgcg ttgaaagggc tgtctcaggt agtgtacccg gtaagagtcg
     3841 ccatcgagaa attccacgat ggcaaggata aaatcctcgg gcttgttgag ggagtagagg
     3901 atttcattgc gggtcaccgt gaccgtcggt gcaccggata ctcgtccttt cacctcgatg
     3961 aaacgcaatt ttcccgtgcc cgggatacag ctttcgatat cgtaacccaa tttctcaagt
     4021 tcgcggtcgg tcggctcaaa cccgaggttg cgttcgacct ccataatgat ggcgcgggct
     4081 cgggcggcac tggcttgtgt atcagcaggc gcggtcgata ttgccgcccc ctgacctgtc
     4141 atggccgtca gtaaaccttt gggcacgatc agcattccac ccaacacgac cggtggtagc
     4201 ggcgagatct gagcttcgag tttcaggtct tcgagtcgct tttgcagccg cccctgaagc
     4261 agatcggccc gcttacgggc ctcacccgaa ttgagccgat tacctgttct gcctgcctgt
     4321 tccaagagtt tgagctgctc ggcgcgatga tcccaatagg taatctcctt ggtgagccgc
     4381 tccttcaccg ccgcttctgt cttggcaatc agtttgagct tggggcctcg cacctccgcc
     4441 aagtgttccg gaacgacgtt cactatcgcg tgaccctgtg ccttcttctc cagctcgcgg
     4501 gtaatccagg tgcactcggg cctgttgagg attgcttcaa cagtcggttc gcctttggcc
     4561 agcggccggt aatcgagata gggtgcataa ttcacatggt gtgtggcgcc atcggcgtca
     4621 agctctatat atagcatccg cctggagacg acgcggcgct ctcccgaccg tgtcaggctt
     4681 gcgtcctgga tagtatgttc gagatagaac atcacgtgcg gctggatacc cgtgtcgcgt
     4741 tcatctacca gcacggcgcc gcgtttcaag agatctcggt ggcgctcaag ggtaaggtcg
     4801 ataaccgaat caagcaacgg atgcccagga caaacaaagg cagccagtgg cagaccttga
     4861 ggcgctacca gcggcttctc aaaggcaatg cgttcatatc ggggcagcac cggttcgccg
     4921 ataccgatca aacggtcacg gttccgcacc ggggccggga catgggtaac ctcgtagcgg
     4981 cgcggctcac gctgtctggc cgatcctccc agatagcgga aggcttcgag gaaaaaagac
     5041 tcgatatagt gcggctgaag gcgtctggct tctgcccgct ccatatcctc ccggatatgt
     5101 tggatgcgac tcgcgtccat tgcatcgtgg gcaagagcat ggtcttcaag aaggtcccgg
     5161 agctggtcgc gatccaaagc atggtcaata acggtggtca ggtaggcttt aacctctggg
     5221 cgctcgccat aacggacagc ctcaatcagc agatcccgca acagtttacc ctcgaattgg
     5281 agcttgccga gcacgtcgaa tacctggccg ccgagggatt gacgggcctg ttccagtttg
     5341 tccagaagct tgcgataaac gtctccctca cgggtttctt cggccaccag gttccagaga
     5401 tggcatacct cggtctggcc gatgcggtgg atacggccaa aacgctgctc gatgcggttg
     5461 gggttccacg gcaggtcata gttcaccatc aaatgtgcac gctggaggtt gatgccctcg
     5521 ccggcggcat ccgtggccaa cagaacctgc actttggggt catgcttgaa agactcctgc
     5581 tccttcatgc gttcctcgcg gcccatgccg ccgtggatca taaccaccgc ctccttacgt
     5641 ccgagcagcg tggtgatacg gttctcaaga taattcaggg tgtcgcggtg ctcggtgaag
     5701 agtacaagct tctgttgtaa ggatggtgtg tgacggggaa tcgctccggc gccgaaagga
     5761 gtaatcggtt cggctaatct gttcgaaatt gcggcggtgg tgaaaatctc acccagaagg
     5821 gttgaaagct cgcgccattt ggtatccgta ccgctgcgac ggacaccgag cgcgagcgac
     5881 tccagacctt tgagggtctc aatctccgct tttaactcgg tgatggttcg cgccgccgtt
     5941 gcctgatcga ggatttcctc ctcggcggcc ttgacctcat tgtccggtgc atcttcgaga
     6001 tcctcgacgt cctcggcatc cagagccagc aggtcggcgg caaggatcgg cgcggcttgg
     6061 cctccgcgtt ggagcacttc catctcacgc agtctgcttt ctaagcgttc tcgtcgtcta
     6121 cgcaatgatt gatagatcgc ttcgggcgag gaagcaagcc gtcgttgcag aatagtgagg
     6181 gcgaagccca cagttccggt acgtttatca ttctgtagcg cctcggcacg gttgaattcc
     6241 tcgcgaacat attcggtgac cgctttgtag agcttcgctt cggcctccga gagtttgtag
     6301 ggaacggtgt aagcgatgcg ctcagggaat agcggcgtac cgtcgaactt gagcaggctc
     6361 tccttcacca tccggcgcat caggtccgag acgtcggcta catgaacccc gtcgcggaag
     6421 cggccttcga aacggtcgcc gtcgaggagg gccataaaga gctgaaagtc agcctcctta
     6481 ccgttgtgcg gcgtcgccgt catcaagagg aagtgccgtg tgagggtcga aagaagctgg
     6541 ccgagccggt atcgcttggt gtatttagtc tccgtgccga aaatggtggc agacatcttg
     6601 tgcgcctcgt cacagaccac aagatcccag cgacaatccg gcgtctggag tttttgctgt
     6661 acgtcctcat tgcgcgagag cttgtcaagc cgggcaatga caaggttggt ctcaaggaac
     6721 cagttgccgg tgcgtgccgc ttggagcttg tcgttggtga gaatctcaaa gggcagatga
     6781 aatcggtggt agagctcgtc ctgccactgc tcggcgaggc tgcccgggca gacgattaga
     6841 cagcgctgta aatcgccgcg ggcgaccagc tctttgatga gcaggcctgc catgatcgtc
     6901 ttgccggcgc cgggatcgtc ggcaaggaga aagcgcagcg gttgacgcgg cagcatggcc
     6961 tcgtaaacgg cggtaatctg gtgcggcagt gggtcaacaa tagaggtgtg gacggcgagc
     7021 accgggtcga agaggtgggc aaggcggatg cggtgcgctt cggaaaccag acggaacagc
     7081 gctccgtcgc cgtcgaaact ccaggggcgg ccttgctcca cgagatcgag acgtggctcg
     7141 tcgtatcgat agagaagttc gtttgccacc ttgcccgtgg gcgtcttgta ggttaattcg
     7201 agcgcctcgg agccaaacca ctgcacgctg acaaccgtga taaggcagtc cggaagaagg
     7261 ccacatactg cggcgtttgg ttgaaggtct tcgagacgaa tcattcggat ttttatttag
     7321 cagcgatgtt ccggcgtgag ttcctgatgt cgttcttact tgcctgatga aagtattctt
     7381 caaaacataa aaccccgctc tcttctggag acacggggtt tcagtaatca gtccatagca
     7441 tcctcacgct gtgttgacca gtttaggatt cttaatcttt atagtcacat atgcgacacc
     7501 gattatcata atataattaa gattgctaca gaaaaacgag aaataagacg aaggactctt
     7561 caagaggcaa agaccgggtt ctttatgaaa ataatagtag tcagacatgt gcgtctttcg
     7621 aaccgcattc aatattttta tcaaaatggc caagcattga cttgtatttg ctttcagtaa
     7681 ggctgatttt caattctgta tcgtatgcca tgcctcccgt tcatctacga gtcaataaaa
     7741 ctacgactcc tgaaaagcaa tgaatacgct tattcgaaat ggagcaattt ttttatcacg
     7801 ggaggtgggt aaagatctgt acccaatctt atttgacgac atcgtaaaaa gcgccctagc
     7861 ccgccgactg ccgggcctgt ataaaagaag taagtcgagt ctgtgccatg aaaccggccc
     7921 aaaaccgtca aacacgtatc taaagaatcc aattagttac gtgcgactta cttagaagtc
     7981 catatgcggc gttgcgcttc atccttcgtc actgcgacgt acgcacaagt
//
LOCUS       DAOSIM010000078         7981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_8398_length_7981_cov_8.566364, whole genome shotgun sequence.
ACCESSION   DAOSIM010000078 DAOSIM010000000
VERSION     DAOSIM010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7981
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8398_length_7981_cov_8.566364"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="PLR47_04850"
     CDS             <1..1076
                     /locus_tag="PLR47_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOG12303.1"
                     /translation="ISAGRSRFSELFNQLKSGVAIYQAIDGGRDFEILELNDAARRIT
                     KVGPNAEGGSVLAVYPGLANHTILATFQNVWRTGNPEEHPTFLYQDDRQTFWADNYVF
                     KLPSGEIVSVFDDVTARKLAADKISRQTRLLSAINRLFAETLKSQDTEAVARTCLAAA
                     LSITDSEFGFIGEVGPDGLFSTYTVSETGWDACSMPQKEATASLQNMIIRGIWGQVIL
                     REQPLIVDDPGAFPDRVGVPEGHPPLTSFLGVPLKDGRKTIGMIALANRTGGYTGEQR
                     EDLEELAVSLVEALRRNQAEEKVKNMYQELEKRVIERTGELVAKTEELERINRVFVDR
                     ELKMRELKARIAELEKKATSDER"
     gene            1085..2944
                     /locus_tag="PLR47_04855"
     CDS             1085..2944
                     /locus_tag="PLR47_04855"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG12304.1"
                     /translation="MVYLDLIFNLSLLVALSVVSGFLDKRFSRDTRTGALLQGALFGA
                     VAIVGMLRPLTLGPGLIFDGRSVMLSLCALFFGPPAAAVSCVMALACRISIGGAGLIM
                     GILTSLSSTAIGLIFFYQNKSSLQPPSAKLLYVMGLAVHAAVAALMFTLPESLFWSTF
                     QNLAAPILLLFPLATLLAGKILSDHLSMMQSASALSESEERCKSLFEASLDAVMLTAP
                     DGRILSANPAACRLLDRSEEELIDLGRGGIMDREDPRLAGALAERERTGKFSGELTMI
                     RKDETKFPVEITTAVFHDRFGQARTSMIFRDITRRLREEEHLKKLQALLAETQRLSKI
                     GGWERDLTTGRGQWTEEVYNIYGISPDAQPSTQEAMDFYAPEDRPVITEAFGRLLETG
                     KPYDLELRFNKATGETIRVRTIGQAEWRDGKIVRVFGNIMDVTERVRAQEALRESEEL
                     FRNLFMHHTAVKLILDPETGDIIDANKAAAEFYGWPRERLLQMKIFDINTLTEEEIRT
                     GLRKVKNEKRTRFQFRHRRADGSIRDVEVFSSRIDVRGKPFLHSIITDITERKEMENR
                     MRQMNEELEQKVQERTAELKKTINQLEETNKVFVGRELKMAELKKRIEELETK"
     gene            2988..5201
                     /locus_tag="PLR47_04860"
     CDS             2988..5201
                     /locus_tag="PLR47_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG12305.1"
                     /translation="MILDTRTIVLSYIATDIACLAVMVLLWRQNRKRISGTHLWVIYY
                     IVSLFALILMILRGAIPDGASVILVNALFMAGTLIGYQALLRFAGEIRRQTFNYFILA
                     AAILIHAYFTFGKPDVNARIFNLSAVLLVFFSQCAWFCLRGAPEAQRKLMWWPGLVFV
                     LHILVSLCRMAAAVFAPHAQADLFQQNTTIALSVIAYQLLFVALTFALALMYNQRLVE
                     EVRANEEKFELAFQSAPYTLVLSRLSDGKILAINDSFTRITGYSEADIRGKTAADLKL
                     WVNEEDRAIVVRDLRETGAVFERKLLFRKKSGEIMTGLLSAKWITIQDEKCLLFSVND
                     VTEREKVLEELRTAQEQWQKTFDSTQDAIWLMDRNHNILRSNKAAQRLFDRPAENMTD
                     RHCWEVVHGTSAPTPECPILLARESLRRESMDLSIAGKWYLVTVDPILDKEERFAGAV
                     HTMTDISERRQAQEEINHLNETLEQKIADQTRDLRDSQAALLNLVDDLNENAKNLTMI
                     NDSLEEVNRELASFSYSVSHDLRAPLRTIDGFSNALMEDYSDRLDAEGNKYLERIHHA
                     TQQMNRLIDDLLGLSRVMKSDFYRQDFNLSEVVREICADFHQQPSEGRPDLVIQDGIA
                     VNADQRMMRIVMTNLLDNARKFAGKKERPILEFGSFVENGETVFFVRDNGVGFNMTYA
                     QRVFEPFERLHRADEFPGTGIGLATVARVISRHRGRIWAEAQEGKGATFYFTLGA"
     gene            5204..6595
                     /locus_tag="PLR47_04865"
     CDS             5204..6595
                     /locus_tag="PLR47_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG12306.1"
                     /translation="MPKPLNVLMIEDAEDDELLLTRALKKNGYEPKVERVQTAREMTR
                     ALENSPWDIILCDYHMPAFSGIEAIDLLKNTGLDIPLIMVSGAIGEETALDCIHRGAS
                     DYIMKGNLTRLGMAVKRGLEEKAFRDHHRQDEEALRRSEEKYRTILESIGDGYYETDL
                     AGSFTFFNDALQRIWGYPKEELLGLNNRQYMDGETAKMLYQAHNRTYKTGIPGKLMDY
                     EIIRKDGGRIAIQQSFTLLKDSLGNTIGFRGVVRDITHLKALEQEREETLGKLRQTLE
                     STVHAMAVAVESRDPYTAGHQRRVTELAYAIAATMGLEPNRINGLRMASMIHDLGKIS
                     IPTEILTKPTKLTKIEFEIVKTHAEAGYEILKDIAFPWPIARIVWEHHEKIDGSGYPR
                     HLKGDDLLTESKILTVADVVEAMASHRPYRPSLGLQDALEEITRNRGILYDADAVAAC
                     LTLFEEKRFSFAI"
     gene            6713..>7981
                     /locus_tag="PLR47_04870"
     CDS             6713..>7981
                     /locus_tag="PLR47_04870"
                     /inference="COORDINATES: protein motif:HMM:NF022783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HOG12307.1"
                     /translation="MASPKSGTTTSPWTDWQKQLNEYLVDSAQRSIIFADILRKRGNN
                     YIKHIRGGQPPVLTFNYEMILNAKHFERPVNYALVRISDRRRKDIDPEQQNRRRALRE
                     RPDIPKRPIVIIDPRAGHGPGIGGSKRDSEIGMALDHGHPVYFVLFYTDPLPGQTLPD
                     VIAAIERFIEKVTQLHPDADAPAVIGNCQAGWAAALACADKPGIAGPLALNGAPLSYW
                     SGVEGVNPMRYRGGLLGGVWVNSFFSDLGNGVFDGANLVLNMELLNPANTYWTKQYNV
                     YSKADTEEERYLNFEKWWGGFFMMNDDEIHFIVRNLFVGNKLERGELELREGRKVSLK
                     DIESPILVFASMGDNITPAQQALNWIPRVYSSVEDIKRHGQVIVYIVHQEIGHLGIFV
                     AGSIAKKEHHEIIGNFDMIDCLPPGLYEMVI"
BASE COUNT         1845 a         2301 c         2282 g         1553 t
ORIGIN      
        1 aaatttcggc cggccggtcg cgtttcagcg aacttttcaa ccaattgaaa agcggcgtag
       61 ccatctatca ggccatcgac gggggaaggg attttgaaat tctggaactc aacgacgcag
      121 cccgccgaat caccaaggtg ggcccaaacg cagagggcgg aagcgtcctg gccgtctatc
      181 cgggactggc gaatcacacg atcctggcca cttttcaaaa cgtctggcgg acgggaaatc
      241 ccgaagagca ccccaccttt ctttatcagg acgaccggca gaccttttgg gcggacaatt
      301 acgttttcaa acttccttcc ggcgagatcg tttccgtttt cgacgacgtg accgcgcgca
      361 aactcgcggc ggataaaatt tcaagacaga cccgcctgct ttccgccatc aaccggcttt
      421 ttgccgagac gctgaaatcc caggacactg aagcggtcgc aaggacctgc cttgccgccg
      481 ccctgagcat tacggacagc gagttcggat ttatcggtga agtgggcccg gacggacttt
      541 tttccacata caccgtcagc gaaacgggct gggacgcctg ctccatgccc cagaaggagg
      601 caacggcttc tctgcaaaac atgatcattc ggggcatctg gggacaggtg atccttcggg
      661 agcagcctct aattgtcgac gatccgggag ctttccccga ccgcgtgggc gttccggagg
      721 ggcatccgcc gctgacatct tttctgggcg tgccgctcaa agacgggaga aaaaccatcg
      781 ggatgattgc gctcgccaac cgcaccggcg gctacacggg cgagcagaga gaagacctgg
      841 aggagctggc ggtttccctg gtggaggccc tgcggcgcaa ccaggcagaa gaaaaagtca
      901 aaaacatgta tcaggagctg gaaaaacgcg tcatcgagcg caccggggag ctggtcgcca
      961 aaaccgaaga actggagcgg atcaaccgcg tctttgtgga ccgcgagctc aaaatgcggg
     1021 aactcaaggc ccggatcgcg gagttggaaa agaaagcgac gagcgatgag cgatgaggtt
     1081 ttaaatggtt tacctggatt taatattcaa tctgtcgctt ctggtggcgc tcagcgttgt
     1141 ttcggggttt ctcgataagc gcttttcgcg cgatacccgg acgggggcgc tcctgcaggg
     1201 ggcgctgttc ggcgccgtgg ccatcgtcgg catgctccgc ccgcttaccc ttggaccggg
     1261 gctgatcttc gacggccgct cggtcatgct cagcctctgc gctttatttt tcggtcctcc
     1321 ggctgccgcc gtctcgtgcg tcatggcgct ggcctgccgc atcagcatcg gcggcgcggg
     1381 gctgatcatg ggaattctga catccctttc ttccaccgcc ataggactga tcttttttta
     1441 ccaaaacaaa tcctcgctgc aaccgccttc cgcgaagctt ctctacgtga tgggactcgc
     1501 cgtccacgcg gccgtggcgg ctttgatgtt tacgcttccg gaaagtcttt tttggagcac
     1561 tttccaaaac cttgccgctc ccatcctcct cctttttccc ctggccacac tgctggcggg
     1621 aaaaattctt tccgaccatc tttcaatgat gcagtccgcc tcggctcttt cggaaagcga
     1681 agagcgctgc aagtctttgt tcgaagcaag cctggacgcg gtcatgctga cggcccccga
     1741 cggccgaatc ctttccgcca atcccgccgc ctgccgcctg ctggaccgca gtgaagagga
     1801 actgatcgac ttgggccgcg gcggcatcat ggaccgggaa gatccccggc ttgccggggc
     1861 gcttgccgag cgcgaaagga caggaaaatt ttccggcgaa ctgacgatga tccgcaagga
     1921 cgaaaccaaa tttcccgtgg aaatcacgac cgccgttttt catgaccgct tcgggcaggc
     1981 gcgcaccagc atgatcttcc gcgacatcac ccggcgcctg cgggaggagg aacacctgaa
     2041 aaaactgcag gcgcttctgg cggaaaccca gcggctgagc aaaatcggcg gctgggaacg
     2101 tgatctgaca acgggccggg gccagtggac cgaggaagtc tataacattt acggcatctc
     2161 tccggacgct cagcccagca cacaagaagc gatggatttt tacgctccgg aagaccgccc
     2221 ggtcatcacc gaggcctttg ggcgactgct ggaaacgggc aaaccctacg acctggaact
     2281 ccggtttaac aaggcaacgg gagaaaccat tcgtgtgcgc accatcggac aggcggaatg
     2341 gagggatgga aaaatcgtcc gggtcttcgg caacatcatg gacgtcaccg aacgcgtgcg
     2401 cgcgcaggag gcgcttcgcg aaagcgaaga acttttcaga aacctgttca tgcaccatac
     2461 ggcagtcaaa ttgattcttg atccggagac gggggacatc atcgacgcca acaaagccgc
     2521 cgcggaattt tacggctggc cccgcgagcg gcttttacaa atgaaaattt ttgacatcaa
     2581 cacgctgacg gaggaggaaa tccgcacggg gctgcgcaag gtcaagaatg agaaacgcac
     2641 acgatttcaa ttccgccacc ggcgggccga cggctccatt cgcgacgtcg aggttttcag
     2701 cagccggatc gacgttcggg gaaagccgtt tctgcattcg atcatcaccg acatcaccga
     2761 acgaaaagaa atggaaaacc gaatgcggca aatgaacgag gagctcgaac aaaaggttca
     2821 ggagcgaacg gccgagctga aaaaaaccat taaccaactt gaagaaacaa acaaggtatt
     2881 tgtcggccgg gagctgaaaa tggccgagct caaaaaacgg atcgaagagc tggaaacaaa
     2941 ataaaacacg gcgatgaccg gtcgtcatcc cgcagccgag gcctgtaatg attctggaca
     3001 cccgcaccat tgttttatcc tatatcgcaa cggacatcgc ctgcctggcc gtcatggttc
     3061 tgctctggcg gcaaaaccgc aagcgaatca gcggaacgca tctgtgggtc atctattata
     3121 tagtaagtct gtttgccctc atcctgatga ttctgcgggg cgctattccc gacggggcat
     3181 cggttatcct ggtcaacgcc ctgtttatgg ccggcacgct gatcggttat caggcgcttc
     3241 tgcgctttgc cggtgaaatt cgcagacaaa ctttcaatta tttcattctg gcggcggcga
     3301 ttttgatcca cgcctacttc acgttcggca aacccgacgt gaacgcccga atcttcaatc
     3361 tgtcggcggt gctcctggtc tttttttcgc agtgcgcctg gttctgcctg cggggcgctc
     3421 cggaggccca aaggaagctg atgtggtggc ccggcctggt ttttgttctc catattctag
     3481 tcagtctgtg ccgaatggcc gcggccgttt tcgcccccca tgcgcaggcg gatttgtttc
     3541 agcaaaacac gaccattgcc ctgagcgtga tcgcctacca gcttctgttt gtcgcgctca
     3601 ctttcgcgct ggcgctcatg tacaaccagc ggctcgtcga agaggttcgg gcgaatgaag
     3661 aaaaatttga gctggccttt caatccgctc cttatacgct cgttttgtcc cgcctgtccg
     3721 acggaaagat cctggctatc aatgacagtt tcaccagaat caccggctat agcgaagcgg
     3781 acattcgggg aaagacggcg gcggatctga agctgtgggt caacgaggaa gaccgggcca
     3841 ttgttgtccg ggacctgcgg gaaaccggcg ccgtctttga gcggaaactg ctgtttcgga
     3901 aaaaatcggg tgagatcatg acggggctcc tttccgcaaa atggattacc attcaggatg
     3961 aaaagtgcct gctgttcagc gtcaacgacg tcaccgaacg ggaaaaggtc ctggaagaac
     4021 ttcgcaccgc gcaggagcag tggcagaaaa ctttcgattc cacccaggat gccatctggc
     4081 tcatggaccg gaatcacaac atcctgcgtt cgaataaagc cgcccagcgc cttttcgatc
     4141 gtccggcgga aaacatgacc gatcgacact gctgggaagt cgtccacggc acgtcggcgc
     4201 cgactcccga atgccccatt ttactggcca gggaatccct gcggcgtgaa tccatggatt
     4261 tatccatcgc ggggaaatgg tatcttgtca cggtggaccc gattctggac aaagaggagc
     4321 gttttgcggg cgccgtccac accatgaccg acatcagcga acgcaggcag gcccaggagg
     4381 aaatcaacca cctcaatgag accctggaac agaaaatcgc cgatcagacc cgtgatctgc
     4441 gcgacagcca ggcggcgctt ctgaatctgg tggacgatct caacgaaaac gcgaaaaacc
     4501 tcaccatgat caatgactcc ctggaagagg tcaaccggga gctggcgtcc ttcagctaca
     4561 gcgtctcgca cgatctgcgg gcgccgctgc gcacgatcga cggattcagc aacgccctta
     4621 tggaagacta cagcgaccgg ctggatgcgg aaggtaataa atatctggaa agaatccatc
     4681 acgccacgca gcagatgaac cggctgatcg acgacctgct cggcctctcg cgggtgatga
     4741 aatccgattt ttaccggcag gatttcaatc tgagcgaagt ggtacgcgag atttgcgccg
     4801 actttcacca gcagccttcg gaaggccggc cggatctggt aattcaggac ggcatcgccg
     4861 tcaacgcgga tcagcgcatg atgcgcatcg tcatgaccaa cctgctggac aatgcccgga
     4921 aattcgcggg gaaaaaggag cggccgatcc ttgaattcgg ctcttttgtc gaaaacggcg
     4981 aaacggtctt ctttgtccgg gacaacggcg tgggattcaa catgacctac gcccaaagag
     5041 tttttgagcc ttttgaacgc ctccaccggg ccgatgagtt tccgggaacg ggaatcggcc
     5101 tggcgacggt cgcacgcgtc atttcccgcc accgcggacg catctgggct gaagcccagg
     5161 aaggaaaagg agcgacattt tatttcaccc tgggagcctg aaaatgccaa aacccctgaa
     5221 cgtgctcatg attgaagatg cggaagacga cgagctgctt ctgacccgcg cactgaaaaa
     5281 aaacggctat gaaccgaaag tcgagcgggt gcagacggcc cgggaaatga cccgggccct
     5341 ggaaaacagc ccgtgggata tcatcctgtg cgactaccac atgccggcct tcagcggcat
     5401 cgaagccatc gacctgctga agaacaccgg cctcgatatt cccctgatca tggtatccgg
     5461 cgccatcggc gaagaaacgg cgctggactg catccaccgc ggggcgtcgg actatatcat
     5521 gaagggcaat ctgacgcggc tgggcatggc cgttaagcgg gggctggaag aaaaagcatt
     5581 tcgcgaccat caccggcagg acgaggaggc gctgcgccga agcgaggaaa agtaccggac
     5641 aatcctggaa agcatcggcg acggctatta cgaaacggac ctggccggca gctttacgtt
     5701 tttcaacgac gccctgcagc gaatatgggg ctacccgaaa gaagaactgc tgggcctgaa
     5761 caaccggcaa tacatggacg gggaaacggc caaaatgctg tatcaggccc acaaccggac
     5821 ttacaaaacg ggcatccccg gaaaattgat ggactacgag atcattcgga aggacggcgg
     5881 gcggatagcg atccagcagt ctttcacgct gctgaaagat tccctgggca acaccatcgg
     5941 cttccggggg gtcgtgcgcg acatcaccca tctgaaagcg ctggagcagg agagggagga
     6001 aaccctggga aagcttcggc agacgctgga gtccaccgtt cacgccatgg cggtggccgt
     6061 ggaatcgagg gatccctaca cggcgggcca tcagcggcgg gtgacggagc ttgcctacgc
     6121 cattgccgcc acgatgggac tggagccgaa ccggatcaac ggtctgcgca tggccagcat
     6181 gattcatgac ctgggaaaaa tatccattcc caccgagatt ctcaccaagc cgaccaaact
     6241 gacgaaaatt gaatttgaaa tcgtcaagac gcacgcagag gcaggatacg aaattctgaa
     6301 ggacatcgcc ttcccctggc ccatcgcgcg gatcgtttgg gagcatcacg agaagatcga
     6361 cgggtccggt tatccccggc atttaaaagg agacgatctt ctgacggaat cgaaaatcct
     6421 gacggttgcc gacgtggtcg aagccatggc gtcccaccgc ccctaccgcc cttctctggg
     6481 ccttcaggat gcgctggagg aaatcacgcg gaaccggggg atcctctacg acgcggatgc
     6541 cgtcgccgcc tgccttactt tgtttgagga aaagcgtttc agctttgcaa tataacgagt
     6601 cgctcgtcat cctcgctcat cgctcgctgc ttctttatcg tcttgacaag ggtcaaacgc
     6661 gccggtacag tacgtcaacc gtgaccggtt aaagaataca ggaggattat tcatggcttc
     6721 cccgaaaagc ggcacaacga cctctccctg gacggactgg caaaaacaac tgaacgagta
     6781 tctcgtggat tccgcgcagc gctcgatcat ttttgcggac attctgcgca agagaggcaa
     6841 caattacatc aagcacattc gaggcggcca gccgccggtt ctgacgttca actacgagat
     6901 gatcctgaac gccaagcatt tcgagcggcc ggtcaattac gcgctggttc gcatttccga
     6961 ccgccgccgg aaagacatcg atcccgagca gcaaaaccgc cgccgcgcgc ttcgggaacg
     7021 ccccgacatt cccaaacggc cgattgtcat catcgatccg cgggcgggcc acggccccgg
     7081 catcgggggc tccaaacggg attcggaaat cggcatggca ctggaccacg gccatccggt
     7141 ttattttgtt ctgttttaca cggatcccct tcccggccag acgctgccgg atgtcatagc
     7201 cgccattgaa cggttcatcg aaaaagtcac ccagctgcac cccgacgcgg atgcgccggc
     7261 ggtcatcggc aactgccagg cgggctgggc ggccgcgctg gcctgcgcgg acaagccggg
     7321 catcgccggc cccctggcgc tcaacggcgc gcccctgtcc tactggagcg gtgtcgaggg
     7381 cgtcaatccc atgcgctacc ggggaggcct tctgggcggc gtgtgggtca attcattttt
     7441 cagcgatctg ggcaacggcg tgtttgacgg cgcgaatctg gtgctgaaca tggagctgtt
     7501 gaatccggcc aacacatact ggaccaaaca atacaacgtt tattcaaagg cagacacgga
     7561 agaggagcgc tatctcaact ttgaaaaatg gtggggcggc ttcttcatga tgaacgacga
     7621 tgaaattcac ttcatcgtcc gcaatctttt tgtcggcaac aagctggagc gaggcgaact
     7681 ggagctgcgg gaaggccgga aggtcagcct gaaagacatc gaaagcccga tcctggtttt
     7741 tgcgtcgatg ggcgacaaca tcacgccggc ccagcaggcg ctcaactgga ttccccgcgt
     7801 gtacagctcc gtggaagaca tcaaacggca cgggcaggtc atcgtctata tcgttcacca
     7861 ggaaatcggc cacctgggca tcttcgtcgc cggctccatc gccaaaaagg agcaccatga
     7921 aatcataggc aatttcgaca tgattgattg cctgccgccc ggcctctacg aaatggtcat
     7981 c
//
LOCUS       DAOSIM010000079         7965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_8421_length_7965_cov_11.886473, whole genome shotgun sequence.
ACCESSION   DAOSIM010000079 DAOSIM010000000
VERSION     DAOSIM010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8421_length_7965_cov_11.886473"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PLR47_04875"
     CDS             <1..617
                     /locus_tag="PLR47_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOG12308.1"
                     /translation="LHGSLLPSYRGRCPVNWVLVNGEKETGVTLHCMVEKPDAGDIVG
                     RRAVSIDFEDTARTLYDKLCEAAGGLLDELLPEMKKGKIPRIKQDLSRGSYYGGRKPE
                     DGRISWDRTAVEIYNLIRAVTDPYPGAFAFAENGGKILIWRARPVSSVSGGGPGDVIL
                     DGQSVLVKTGDGAIRLQEIEASGARWKDAGIGNYFKQGKVKKLT"
     gene            614..1648
                     /locus_tag="PLR47_04880"
     CDS             614..1648
                     /locus_tag="PLR47_04880"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HOG12309.1"
                     /translation="MKILILGVNGFIGHHLVNRILAEKPDWQVFGMDLGTERIGAALH
                     NPRFNFLEGDISINREWIEYHVKKCDVIMPLVAIATPKAYVEDPIGVYNLDFEMNVNI
                     VKQCVKYRKRVVFPSTSEVYGACSDPEFDEDESYLSVGPIRKERWIYSCCKQLLDRVI
                     YAYGTRGSLEFTLFRPFNWIGPRLDSLDTAKEGSSRVVTQFIAELLLKRPINLVDGGR
                     QKRCFTYVDDGIAALMKILENKGDVCKNQIINIGNPDNECSVKELAALLKKLFQEHPD
                     HQQDSVYSDIIEIPADVYYGKGYQDIYTRKPRIEKARKLLGWEPKIGLQESLRLTLNS
                     FLEENQAATL"
     gene            1672..2529
                     /locus_tag="PLR47_04885"
     CDS             1672..2529
                     /locus_tag="PLR47_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOG12310.1"
                     /translation="MGRLGLKIDVDTYAGMKNGVPRLLRVLNDFSVRGTFFLSIGPDN
                     SGRAALQMIKNPLFLKKMLRTRAPSLYGWKTALYGTLLPAPMIALSFPGLVRQILAEG
                     HEVQFHAWDHRRWQDDLPEKSEAWIEEWFDRGLKGFEKLTGQKPAAFGAPSWLIDDRV
                     LRIIRKYPFAYLSCTRAKAPFVHDPLNLPEIPSDLPCFEELDLQNGPAVILGLMNDGG
                     DHVLPVHAEVEGGVFEEQFRRLLQASLKAGMEVTGLGDFKNALTMDRLPRRKYKMELL
                     PGRHSLCAV"
     gene            2745..3599
                     /locus_tag="PLR47_04890"
     CDS             2745..3599
                     /locus_tag="PLR47_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOG12311.1"
                     /translation="MTKISLSGKLIYGLIAGSILAVLLTLYIIYDYAGITRDRMELSR
                     LRQQTAQQTKEMNELAIKIDQFADRMEEIKQFDKKLRILATEQIGQDAKTPLGIGGSN
                     NDQMRLRQLIEEDREKLVSGMHENIDRLNAEADEREMSFNELLRFLREQKSLLAATPS
                     LWPVHGWVTSEFGMRRNPFGSGGEFHKGIDIASRAGKEIIAPADGFVLEAAYRTDDGN
                     VVKIEHGYGISTSYAHLSKAAVKDGVQVRKGDVIGYVGDTGRSTGAHLHYSVFVNNVP
                     VNPRKYLK"
     gene            3764..6283
                     /gene="secA"
                     /locus_tag="PLR47_04895"
     CDS             3764..6283
                     /gene="secA"
                     /locus_tag="PLR47_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOG12312.1"
                     /translation="MLNAILKKIVGTKNDRELKRLSVLLHEVNHHEKELMSLGDAELK
                     AKTSAFREKLKNGLTLDDILPEAFAVAREASRRTLMMRPFDVQVIGGIVLHEGKIAEM
                     KTGEGKTLAATMPLYLNALSGKGCHVVTVNDYLARRDAEWMSPIYHFLGLTVGVIVHG
                     MDDDERRKAYAADITYGTNNEFGFDYLRDNMKFSLEDYVQREFNFAIVDEVDSILIDE
                     ARTPLIISGASEESTDKYYKINQIIPRLRKDADYTIEEKSRTVVLTEEGVSHVEQLLN
                     VQNLYEPRNIEIVHHVNQALRAHTLFKRDVDYLVKEGQVIIVDEFTGRVMPGRRYSDG
                     LHQALEAKEKVKIEKENQTLASITFQNYFRMYKKLAGMTGTADTEAEEFKKIYNLEVV
                     VMPTNMPMIRQDHNDLIYKTEPEKIKAVIEEVKALNKAGRPVLIGTISIEKSELLSKY
                     LTRAGVKHHVLNAKNHEKEAEIVAQAGQAGQVTISTNMAGRGTDIKLGEKVAELGGLH
                     ILGTERHESRRIDNQLRGRSGRQGDNGSSRFYLSLEDDLLRVFGADRISSVMSTVGIE
                     EGQPIEHKYISRAIENAQKRVEGQNFDIRKHLLDYDDVMNNQRKVIYEQRKKVLRGED
                     LWSDIEAMVEEIAGDLLAEFIGEKNHPQDWNLKSLEDAVFQQFKLRLNIANAQDLTSA
                     DAVRSLILEKVQEYLRAKEKEFGPELMTYLMKVIMLQAIDTHWKEHLLSMDHLREGIG
                     LRGYGQKDPVREYQREGYAMFMEMISRIKMDTLEKLCLVRIQREEEVEEIRQKQKQDY
                     ILSRGEDTPVNQTVKRATEKIGRNDPCPCGSGKKYKKCCGA"
     gene            6305..7504
                     /gene="argJ"
                     /locus_tag="PLR47_04900"
     CDS             6305..7504
                     /gene="argJ"
                     /locus_tag="PLR47_04900"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOG12313.1"
                     /translation="MTKSANKFSVPGFLANGVSAGIKGGDRKDLGLICSTVPARVAAL
                     FTKNTFKAAPVLIDAERVKRGTAQAILTNSGCANAATGKEGMADALAVSAAAAKQLKI
                     PEEHLLICSTGVIGRRLPVRKIEAGIGKLVKGLNESGIEDAEAAMMTTDKFPKISIRK
                     GIVGAKDITICGIAKGAGMIEPNMATLLTYVMTDALIDAKALNTVFHQVIDSTFNAVS
                     VDGCMSTNDTAIILANGLAGNNPLERAQARLQRFREMLTDVMADLSAAVVKDGEGATK
                     FITVVVEGAKSKSDARRVAYSIANSNLVKTAFFGEDPNWGRILSAVGASGILLEKEKV
                     SLSIGNMTVFAQDAPANIAPARLKEIFQGGSIEVRVSLGGGDKSYTVYTSDLSHDYVK
                     INADYTT"
     gene            7686..>7965
                     /gene="rpsB"
                     /locus_tag="PLR47_04905"
     CDS             7686..>7965
                     /gene="rpsB"
                     /locus_tag="PLR47_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOG12314.1"
                     /translation="MSAISMKLLLEAGVHFGHQTNKWNPKMKPYIFGARNNIYIIDLQ
                     QTVGMFQSAYNFVVDTVSQGREILFVGTKKQSQEAIREEATRCGMPYVN"
BASE COUNT         2032 a         2043 c         2299 g         1591 t
ORIGIN      
        1 atctgcacgg ctcgcttctg ccgtcttacc ggggcaggtg tccggtcaac tgggttctgg
       61 tcaacggaga gaaagagacg ggcgtcacgc tgcattgcat ggtggaaaaa ccggatgcgg
      121 gcgacatcgt cggccggagg gccgtgagca tcgattttga ggatacggcg cgaacgctgt
      181 acgacaagct ctgcgaggcg gccggcgggc tgctcgacga gctgctcccc gaaatgaaga
      241 aaggaaaaat tccgcgcatc aagcaggacc tgtcacgggg aagctattac ggcggacgga
      301 aaccggagga cgggcgcatc tcctgggacc gaaccgccgt cgaaatctac aatttgatcc
      361 gcgccgtgac ggacccctat ccgggagcgt ttgcctttgc cgaaaacggc gggaaaattc
      421 tgatctggcg ggccaggccg gtttcatctg tttccggggg cggaccgggc gacgtgatcc
      481 tcgacgggca atccgtgctt gtgaaaaccg gcgatggtgc tataaggctt caggagatcg
      541 aagcatccgg ggcaagatgg aaggatgcgg gcatcgggaa ctattttaaa cagggaaagg
      601 tgaaaaaact tacatgaaga ttttaatact gggcgtcaac gggttcatcg ggcatcatct
      661 tgtcaaccgg attctggctg agaagccgga ctggcaggtg tttggaatgg atctgggaac
      721 ggagcggatc ggcgccgccc tgcacaatcc ccgcttcaat tttttggaag gggacatttc
      781 catcaaccgg gaatggattg aataccatgt caaaaaatgc gacgtcatca tgccgctggt
      841 ggcgattgcc actccgaagg cctacgtgga agaccccatc ggcgtctata acctggattt
      901 tgaaatgaac gtcaatattg tcaagcagtg cgtcaaatac cgcaagcgcg ttgttttccc
      961 ctctacgtcg gaggtttacg gggcctgctc cgatccggag tttgacgaag acgaaagcta
     1021 tctgtcggtc gggccgattc gcaaggagcg ctggatttat tcctgctgca agcagcttct
     1081 ggatcgtgtg atttatgcct atggaacgag gggaagcctg gaattcacgc ttttccgtcc
     1141 cttcaactgg atcgggccga ggctggattc gctggatacg gccaaggaag gaagctcgcg
     1201 cgtcgtcacg cagttcatcg ccgagctgct gttgaagcgg ccgatcaatc tggtggacgg
     1261 cggccggcag aagcgctgct tcacttacgt ggacgacggc atcgcggcgc tcatgaagat
     1321 tctggaaaac aaaggagatg tctgcaagaa tcagatcatc aacatcggca atccggacaa
     1381 tgaatgctcg gtgaaggagc tggcggcgct gctcaaaaag ctgtttcagg agcaccccga
     1441 ccatcaacag gattcggttt attcggatat tattgaaatt cccgcggacg tctattacgg
     1501 aaaaggctac caggatattt acaccagaaa accccgcatc gaaaaggcca ggaaactgct
     1561 cggctgggag cccaaaatcg gtttgcagga atccctgcgc ctgacgctca attcgtttct
     1621 ggaggaaaac caggcggcaa cgctttaacc ggattgaatt cggaaggatc gatgggcagg
     1681 cttggattaa aaatcgatgt ggacacgtat gcgggaatga aaaacggcgt tccccgcctg
     1741 ttgcgcgtcc tgaatgattt ttccgtccgg gggactttct ttctgagcat cgggccggac
     1801 aattccggac gcgccgcgct gcagatgatt aaaaaccccc tgtttctgaa aaaaatgctg
     1861 cgcacccggg cgcccagtct gtacggctgg aaaaccgccc tttacgggac gcttcttccc
     1921 gcgccgatga tcgcgctttc ctttcccggg ctggtccgtc aaatccttgc cgagggccac
     1981 gaagttcagt ttcacgcctg ggaccaccgc cgctggcagg atgacctgcc ggaaaaatcc
     2041 gaggcgtgga tcgaagagtg gttcgacagg ggcctcaaag gctttgagaa gctcacggga
     2101 caaaagccgg ccgcgttcgg cgccccttcc tggctgattg acgaccgcgt tttgcggatc
     2161 attcggaaat atccgttcgc atatctgagc tgtacgcggg ccaaagcgcc gtttgttcac
     2221 gatccgctca atcttccgga aatcccctcc gatctgccct gttttgaaga gcttgatctt
     2281 caaaacggcc cggccgtgat cctgggcttg atgaacgacg ggggcgacca tgtccttccg
     2341 gtccacgccg aagtggaagg aggggtcttt gaggagcagt tcaggcggct tttgcaggcc
     2401 tcgctgaagg cggggatgga agtgacgggg cttggcgatt tcaaaaatgc cttaaccatg
     2461 gacaggctcc ccagacgaaa atacaaaatg gagctgcttc cgggaagaca ttctttgtgc
     2521 gcggtatgaa acaggacggc ctggataaca caagtcattg aattagcttg ataataattg
     2581 tttagtcgca caaaggcgcc aagacttatt cttgactttg atttttccct gtgttagggg
     2641 atgcaaaact ttatacctgt tcatcctctt cacttcagct tttcaggggg aaaatgtccg
     2701 ataatttttt tacactgatg attatacccc gaagaaagag ttctgtgacc aagatttctc
     2761 tttcgggcaa actgatctat ggccttattg ccggttccat cctggcggtt cttctaacgc
     2821 tttacattat ttacgattat gccggaatta cccgggaccg gatggagctg tcccgccttc
     2881 ggcagcagac ggcccagcag acaaaagaga tgaatgagct ggccataaaa atcgatcagt
     2941 ttgccgaccg catggaggag atcaagcaat ttgacaaaaa attgcggatt ctggcaaccg
     3001 aacagatcgg gcaggacgca aaaacaccgc tgggcatcgg agggtccaat aacgatcaga
     3061 tgcgcctgcg tcagctcatt gaagaggacc gggagaaact ggtttccggc atgcacgaaa
     3121 acatcgaccg gctgaacgcg gaagccgacg agagagaaat gagcttcaac gagcttttaa
     3181 gatttctccg ggagcagaaa tcgcttttgg ctgcgacgcc ttccctgtgg ccggtccatg
     3241 ggtgggtgac ctcggaattc ggcatgcggc gcaatccgtt cggaagcggc ggcgagtttc
     3301 acaaaggcat cgacatcgcg tcgcgggcgg gcaaggaaat tatcgccccc gcggacggtt
     3361 ttgttctgga ggcggcctat cgcaccgatg acggcaatgt cgtaaaaatt gagcatgggt
     3421 acgggatttc gacgagctac gcgcatttgt caaaggccgc cgtgaaagac ggcgtacagg
     3481 tcagaaaagg ggacgtgatc ggatatgtcg gcgataccgg ccgcagcacc ggggctcatc
     3541 ttcattacag tgtttttgtg aacaatgttc cagtcaatcc gagaaaatac ttgaaataat
     3601 ccagcccgac aaattgacca gacatcaggc cgcaggcgcc gggcggcatg cgtaagcgca
     3661 gccgtatgcg aaaagaatat gtttcaggca aatgaatgtt tttgttaggc ggctaaatat
     3721 gctaaaatca cccggtaaaa gccggattat ttaaagggat gtcatgctga acgcgattct
     3781 aaaaaaaata gtgggaacca aaaacgatcg tgaattaaaa agattatcgg tgctgctcca
     3841 tgaggtcaat catcacgaaa aggagctgat gtcgctgggc gacgccgagc tcaaggcaaa
     3901 gacttccgcc ttcagggaaa aactgaaaaa cggattaacg ctggacgata ttctgccgga
     3961 ggcgtttgcc gtggccagag aggcttcccg cagaacgctg atgatgcgcc ctttcgacgt
     4021 ccaggtcatc ggcgggattg ttctgcatga aggcaaaatc gcggaaatga aaaccgggga
     4081 aggcaaaacg ctggccgcga cgatgccttt gtatctaaac gcgctttccg gcaaaggctg
     4141 ccatgtggtc accgtcaacg actacctggc ccggcgcgac gcggaatgga tgagcccgat
     4201 ttatcatttt ctgggattga cggtcggcgt catcgtgcac ggcatggacg acgacgagcg
     4261 ccgcaaggct tacgcggcgg acataacata cggcaccaac aatgaattcg ggtttgacta
     4321 tcttcgcgac aatatgaaat tcagcctgga ggattatgtt cagcgggaat tcaatttcgc
     4381 gatcgttgac gaggtggaca gcattctgat cgacgaggcg cgcacgccgc tcatcatttc
     4441 cggtgcgtcg gaagagtcga cggacaagta ctacaaaatc aaccagatca ttccccggct
     4501 gcgcaaagac gcggactaca caatcgaaga aaaaagcaga acggttgtcc tgacggaaga
     4561 aggcgtgtct catgttgaac agcttctgaa cgtccagaac ctctatgagc cgcgcaatat
     4621 tgaaatcgtt caccatgtca atcaggcgct tcgggcccac acgctcttca aacgcgacgt
     4681 ggactatctg gtgaaagagg gtcaggtgat catcgtcgat gagttcaccg gccgggtcat
     4741 gccgggcagg cgctacagcg acggtctgca ccaggcgctg gaagccaagg aaaaggtcaa
     4801 gatcgaaaag gaaaatcaga cgctggcctc catcacattc cagaattatt tccgaatgta
     4861 caagaaactg gccggaatga ccggcaccgc cgacacggaa gcggaggaat tcaagaaaat
     4921 ttacaacctc gaagtggtgg tgatgccgac caatatgccg atgatccgcc aggatcacaa
     4981 cgacctgatc tacaaaaccg agccggaaaa gatcaaggcc gtgatcgagg aagtcaaggc
     5041 gctcaacaaa gccggacgcc cggttctgat cggcacgatt tccattgaaa aatccgaatt
     5101 gctctccaag tatctcaccc gggccggggt aaagcaccat gtgctgaacg ccaaaaatca
     5161 cgagaaggaa gcggaaatcg tcgcccaggc cggacaggcg ggacaggtaa cgatctcaac
     5221 caacatggcc ggccgcggca cggacatcaa gctcggtgaa aaagtcgcgg agcttggcgg
     5281 tctgcatatt ctgggaacgg aacggcacga aagccggcgc atcgacaatc agcttcgcgg
     5341 ccggtccgga cgccagggag acaacgggtc gtcgcgcttc tatctttccc tggaggatga
     5401 tttgctgcgg gttttcggcg ccgaccggat ctcctcggtg atgagcaccg tgggcatcga
     5461 agaaggccag ccgatcgagc acaaatacat ttcccgggcc atcgaaaacg cccagaaacg
     5521 ggtggaaggg cagaactttg acattcggaa gcatctgctc gattacgacg acgtgatgaa
     5581 caaccagaga aaggtaatct acgaacagcg caaaaaagtc ttgcgtggag aagacttatg
     5641 gtctgatatt gaagcgatgg ttgaggaaat tgccggcgat ctgcttgctg aatttatcgg
     5701 cgaaaagaat caccctcagg attggaacct caaatcactt gaggatgcgg tatttcagca
     5761 gttcaaactg cgcctcaata tcgcaaacgc acaggacctg acgtccgccg atgccgtccg
     5821 aagcctgatt ctggaaaaag ttcaggagta tctgcgcgcc aaggaaaaag aattcggccc
     5881 ggaactgatg acgtatctga tgaaagtcat catgctccag gcgatcgaca cccactggaa
     5941 agaacacctg ctgtccatgg accacctgag ggaaggcatc ggcctgcgcg gatacggaca
     6001 gaaggacccg gtaagggaat accagcgcga agggtatgcg atgttcatgg aaatgatcag
     6061 ccgcatcaag atggacacgc tggaaaaact gtgcctggtc cggattcaaa gagaagaaga
     6121 agtcgaagaa atcaggcaga aacaaaagca ggattatatt ttaagccgag gtgaagacac
     6181 gccggtcaat caaacggtga aacgggcaac cgaaaagatc ggccggaatg atccctgtcc
     6241 ctgcggcagc ggcaaaaaat acaagaaatg ctgcggcgct tgagaatgca aaggagtggc
     6301 ggtcatgacg aaatcagcaa ataaattcag cgttcccggt tttctggcca atggcgtatc
     6361 cgcgggcatc aagggcggcg accggaagga cctggggttg atctgttcca ccgttccggc
     6421 aagagtcgcc gcgctgttta ccaaaaacac ctttaaggcg gcgcccgtgc tgatcgatgc
     6481 cgaaagagtc aaacggggaa cggcgcaggc cattctgacc aacagcggct gcgccaacgc
     6541 ggcgacgggc aaagaaggca tggcggacgc tctggccgtt tcggcggcgg cggcaaagca
     6601 gttgaaaatc cccgaagagc atctgctgat ttgttccacg ggggtgatcg gcaggaggct
     6661 tcccgtccga aaaatcgaag ccggcatcgg caagctggtc aaggggctca acgaatccgg
     6721 catcgaggac gcggaggccg ccatgatgac gacggacaaa tttcccaaga tctccattcg
     6781 caagggcatt gtcggcgcca aggacattac gatctgcggg atcgccaagg gcgccggcat
     6841 gatcgagccg aacatggcga cgcttttgac ctacgtcatg accgacgcgc tcatcgacgc
     6901 caaggcgctc aacaccgtct ttcatcaggt gatcgattcc acgttcaatg ccgtcagcgt
     6961 cgacggctgc atgagcacca acgataccgc gattattctg gcaaacggcc tggccggcaa
     7021 caatccgctg gagagggctc aggccaggct tcagcggttt cgggagatgc tgacggacgt
     7081 catggcggat ctgtccgcgg ccgttgtcaa agacggcgag ggcgcgacca aattcatcac
     7141 cgtcgtggtg gaaggcgcca agtcaaaatc cgacgcgcgg cgcgtggcgt attcgattgc
     7201 caattccaac ctggtcaaaa ccgcgttttt cggcgaagac ccgaactggg gcagaattct
     7261 gtccgccgtc ggcgcttcgg gcatcctgct ggaaaaagaa aaagtcagct tatcgatagg
     7321 aaatatgacc gtctttgccc aggacgcgcc cgcgaatata gcgccggcca gactgaagga
     7381 aatttttcag ggcggcagca tcgaagtccg cgtcagcctg gggggcggcg acaagtccta
     7441 cacggtgtac acctcggacc tttcccacga ctacgtgaaa atcaatgccg attacaccac
     7501 ttgagggcgg taaaagaacg ttaattttat tgccgaaaag gttatttgcg cttgcttgat
     7561 ccatggattt atgataattg cccaaaaaat ttcacacatc ccctgattga cggcgggttg
     7621 cttgacgctc aagtccgccg gcaagacgac gggggtggcg gaaaaaaaca aaggagaaaa
     7681 aatttatgtc agccatttcc atgaagcttc tgctcgaagc cggcgtccat ttcggacacc
     7741 agaccaacaa atggaacccc aagatgaagc cctatatctt cggggcccgc aacaacatct
     7801 acatcattga cctgcagcag accgtcggca tgttccagtc ggcctataat tttgtcgtgg
     7861 acacggtcag ccagggcagg gaaatcctct ttgtcgggac caagaagcag tcccaggagg
     7921 ccattcgcga agaggcgacg cgctgcggca tgccttacgt caatc
//
LOCUS       DAOSIM010000080         7910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_8498_length_7910_cov_5.259198, whole genome shotgun sequence.
ACCESSION   DAOSIM010000080 DAOSIM010000000
VERSION     DAOSIM010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8498_length_7910_cov_5.259198"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PLR47_04910"
     CDS             <1..554
                     /locus_tag="PLR47_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG12315.1"
                     /translation="DIMDVALNLIAERGFHDSPMALIAKEAGVAAGTIYRYFESKDEL
                     IIALHRDLESRINEMILKEYPAEESIREKFLYMNRKLLKYFMEHPLHFRYMEQFFNSP
                     YGMSHRKERLLEKSDRNRMMIDIFEQGTRQEILKALPMPALFALAFGPLPSLLRDHIF
                     GFIHLDEEMIEEVTQACWDAIKK"
     gene            570..1766
                     /locus_tag="PLR47_04915"
     CDS             570..1766
                     /locus_tag="PLR47_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG12316.1"
                     /translation="MQIYAIALRMIFISLLAAGFLLNGCSDKGKGGPPPPTEVSVVTI
                     QAKSVATTTELPGRTSAHLVAEVRPQVAGIIQKRLFSEGANVRAGQVLFQIDSSLYQA
                     ALDNAKASLARSEAQLSTIQLKAARLKELLAEKAVSQQDYDDAAAALKQTQADIQFGK
                     ATVEAARINLQHTAIGAPISGRIGRSSVTEGALVTTHQPQPMATIQELDPMYVDVTQS
                     TAEILRLRRQLQEGRIEHSMKHLDGVRLLLEDGSEYPWKGVLQFQDVTVDPTTGSVVL
                     RMVFPNPKGILLPGMFVRTVIQEGIRKNAILVPQQAVSRDLKGNPFAFVVDSKGIVQQ
                     RPLTLDRTVGDAWLATSGLESGERVIVEGILKVKPGAPVRAVSFKPSAKTNGKGQNPA
                     PPDQTR"
     gene            1778..5035
                     /locus_tag="PLR47_04920"
     CDS             1778..5035
                     /locus_tag="PLR47_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG12317.1"
                     /translation="MLSKFFLDRPVFAWVIAIVVMALGVIAIYILPVSQYPQLAPPSI
                     AISSSYPGASSETVENSVTQVIEQKMTGLDNLIYMSASSDSAGGSRLELTFAPGTDPD
                     IAWSKVQNKVQSALPSLPDVVENQGVQVSKSTRNYLMIVGLISEDGSMDGTDLRDYAK
                     SNLEKIMARVPGVGEVEVFGGGYAMRVWLNPNEMTEYHLTVDDVLAALKAYNVEVSAG
                     QFGGAPAVPGQRLNASIVVQNMLSTPEEFAAIPIRTNPDGSVVRIRDVGRTELGTEIY
                     DIQVSYSGKPSAGIAIRQSPGANALDTADAVKAKMAEMSRFFPPGMKVVYPNDSTVFI
                     KVSINEVIKTLVIAIVLVFLVMLLFLGSIRATLIPTIAVPVVLLGTFAVLALFGFTVN
                     MLTMFAMVLAIGLLVDDAIVVVENVERIMTEEGLSPKEATAKSMEQITSALVGIGLVL
                     SAVFGPMAFFPGSTGIIYRQFSITIMSAMLLSVAVALILTPVLCAGLLKPVQAGHQPA
                     ENAASFARPFFLWFDRVFFRLRDRYVGLVGHSLSRKKRYLVVFLVIVAVMGVLFLRMP
                     RAYLPDEDQGILLAQVIMPTGATLEQTGEITTQIQKYFEENEKEAVESLMTITGIGFS
                     GRSQNNGMVFIRLKDWHLRDRADLKAKAIAGRAMFALSGIRNAMVFTFQPPSVIELGT
                     SIGFDFQLLDRGGVGHSALTNAQYQLLGLAAKDPRVAKVRPNSMEDVPEYRIDVDWER
                     AGALGVPISSIHRTLSAAFGSAYANDFIQGGRIKRVYIQADAPYRMLPKDLENLYVRN
                     TQGRMVPFASFATGRWTSGPPRLTRYNGFPSINIWGEPAPGRSSGEAMQAMEEAMEKL
                     PSGFGFDWSGLSYQERMAGTQAPLLYAFSIFVIFLCLAALYESWTIPISILLVLPLGV
                     IGGIIASSARGLANDVYFQIGLLTTLGLTTKNAILIVQFAKSGLERGMGLLEATLEGA
                     KLRFRPIVMTSLAFGFGVLPLAMTTGAGAGAQNAIGTSVLGGMITATLLVVIFSPLFY
                     VLIETLFGRKLKTDAGKTGAPSLFEHLRQTILTGRRKKKSSRTDKADASEVEQHE"
     gene            5028..6422
                     /locus_tag="PLR47_04925"
     CDS             5028..6422
                     /locus_tag="PLR47_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOG12318.1"
                     /translation="MNKKWLLILMVLLAGCTMAPKYERPEAPVPNEWPAGQSYGEEKQ
                     ALETTQPGWREFITDQRLREIIADSLKNNRDLRVAALNVDKARAMYGIGRASLLPSIN
                     ASGVWYRERIPADLSSSGNSVTAEQFNVSLGMASWEIDFFGRIRSLKDQALEQYLATD
                     QASRSAQILLVAAVAQVYWTLAADREALSLVSSTLSAQEAAYKMIRKRHEVGLASELD
                     LRRAQTQVDTARGDVARYTQMVAQDENYLNLLVGSPVQKELLPPNLAGVSPPRDISAG
                     LSSEPLLRRPDVLAAEHRLKAANANIGAARAALFPRISLTTSIGTASAELSGLFKAGS
                     DVWTFAPQIVMPIFDPRVWYAYDVTKIEKEISIAQYEKSIQTAFREVADALAEKGMVN
                     ERIAAQQSLVDALAVAYRLSELRYEKGIDSYLSVLDAQRSLYGAQQGLILLRLASINN
                     RVALYKALGGGASP"
     gene            6419..6856
                     /locus_tag="PLR47_04930"
     CDS             6419..6856
                     /locus_tag="PLR47_04930"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HOG12319.1"
                     /translation="MTKKRRPCSAKEIKYFLLNRVLTSAIVFVLRIYAKTLRVRIEGK
                     EEILKHLAGGGRVIFASWHQRLFGGFFVPRFFQWTPCIMISQSRDGDFISKIVSRLGW
                     IPVRGSSTRGGAKALKAMPRTAAGENDEKTQTRKPNLTNSTWI"
     gene            complement(7028..7753)
                     /locus_tag="PLR47_04935"
     CDS             complement(7028..7753)
                     /locus_tag="PLR47_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOG12320.1"
                     /translation="MLVKSKISAHHFPQGVCLSCGEGNIGKRRYCSVRCRQHLRQRLN
                     TRSGLLQALNTRYATFYFTDTLIVLDVVPHGIREVFRFQTKRTAGTKPADDFGRMSNL
                     LGDAWWAEEKRTTRNYLASRRVLEMAERLAMADGLKRPRWMKAPCVKPESILLLEMSK
                     ADLASRDLDKIIRNAYRRQAKIHHPDAGGTAAAFRRLHAAYQDLLRWADHPTFIRRRG
                     FPDKWYYDGDNKKWIQPVPVRKG"
BASE COUNT         1612 a         2373 c         2259 g         1666 t
ORIGIN      
        1 ccgacatcat ggacgtggcc ctgaatctga ttgccgagcg gggttttcac gattcaccca
       61 tggccctcat tgccaaggag gcgggggtgg cggccggaac catttaccgc tattttgaaa
      121 gcaaggacga actgatcatc gccctgcacc gcgatctgga atccagaatc aatgaaatga
      181 ttcttaaaga atatccggcc gaagaatcca ttcgggaaaa atttctttac atgaacagaa
      241 aactcctgaa atatttcatg gaacatcccc ttcatttccg ctacatggag cagtttttca
      301 actcccccta cggaatgtcc caccgcaagg aacggctttt ggagaaatcg gacagaaaca
      361 ggatgatgat cgacatattc gagcagggca cgcggcaaga gattctcaaa gccctgccga
      421 tgcccgcgct gtttgccctg gctttcggtc cgctgccctc gctgctgcgc gatcatattt
      481 ttggttttat tcatttggat gaagaaatga ttgaagaggt tacccaggct tgctgggatg
      541 ccatcaaaaa ataaaaacag aggtgccgaa tgcagattta cgccatcgcc ttacgaatga
      601 tcttcatcag tcttcttgcc gcgggatttc tcctgaacgg ctgttcggac aagggcaagg
      661 gagggccccc tcccccgacg gaagtttccg tggtgaccat tcaggcaaag tccgttgcca
      721 ccacgaccga actgccggga aggacttcgg cccatctggt cgcggaagtg cgcccccagg
      781 tggccggcat catccagaag cgcctcttct cggaaggcgc caatgtccgg gccggacagg
      841 tgctttttca gatcgattcc tccctgtacc aggcggcgct cgacaacgcg aaagcctcgc
      901 ttgcgcgctc ggaagcgcag ttgtccacca ttcagctcaa agccgcccgc ctgaaggaat
      961 tgctggccga aaaagccgtg agccagcagg actatgatga cgcagccgcc gcgctgaagc
     1021 agacccaggc ggatattcag ttcggaaagg ccaccgtgga agcggcccgc atcaacttgc
     1081 agcacaccgc cattggggcg cccatttccg ggcgcatcgg ccggtccagc gtcacggagg
     1141 gcgcgctggt gaccacgcac cagccgcagc ccatggccac gattcaagag cttgatccca
     1201 tgtatgtgga tgtcactcag tccacggctg aaattcttcg cctgcgccgc cagcttcagg
     1261 aaggccgcat cgaacacagc atgaagcacc tcgatggcgt ccggcttctg ctggaagacg
     1321 gctcggagta tccctggaaa ggcgtccttc agtttcagga cgtcaccgtc gatccgacca
     1381 ccggatcggt tgtcctgcgc atggtatttc ccaatccgaa aggcattctg ctgccgggca
     1441 tgttcgtccg cacggtcatt caggagggaa tccgcaaaaa cgcgatcctc gtcccccagc
     1501 aggcggtttc ccgcgatctc aagggaaatc cttttgcctt tgtcgtggat tcgaaaggaa
     1561 tcgtgcagca gcggcccctc acgctggacc ggacggtcgg cgacgcatgg ctggcaacct
     1621 cgggcctgga atccggagag cgcgtcattg tcgaaggtat tttgaaagta aagcccggcg
     1681 cccccgtcag agccgtttct tttaaacctt ccgcgaaaac gaacgggaaa ggccaaaacc
     1741 cggccccgcc cgatcagacc cggtaacgga ggcgtaaatg ctatccaaat tctttctgga
     1801 ccgtcccgtt ttcgcctggg tcatcgccat cgtcgtcatg gcgctgggcg ttatcgcgat
     1861 ctatattttg cccgtctccc agtatccgca gctggcgcct ccctccatcg cgatttcatc
     1921 ctcctacccg ggagcctcgt ccgaaacggt tgaaaacagc gtgacgcagg tcatcgagca
     1981 gaagatgacc ggtctggaca atctgatcta catgtccgcc tccagcgatt cggcgggcgg
     2041 ctcccgtctg gagctgacgt tcgctccggg aacggacccg gacattgcct ggtccaaagt
     2101 gcaaaacaag gtccagtccg ctctgcccag ccttcccgac gtcgtcgaaa atcagggagt
     2161 gcaggtcagc aaatccacac ggaactatct gatgatcgtc gggctgattt cagaagacgg
     2221 ctcgatggac ggaacagacc tgagggatta cgccaagtcc aacctggaaa aaattatggc
     2281 ccgcgtcccc ggcgtcggcg aggtggaggt tttcggcggc ggctatgcca tgcgcgtctg
     2341 gctcaatcct aatgaaatga cggagtatca tctgacggtt gacgatgttc tggccgcgct
     2401 caaggcctac aatgtggagg tatccgccgg ccagttcggc ggggcgcccg ccgttcccgg
     2461 ccagcgcttg aacgcgtcca tcgtcgtcca gaacatgctt tccactccgg aagaatttgc
     2521 cgccatcccg attcggacca atcccgacgg ctccgtggtt cgcatccgcg acgtgggacg
     2581 aacggaattg ggaacggaga tttacgacat tcaggtttca tacagcggca agccttcagc
     2641 gggcatcgcc atccgccagt cgccgggcgc gaacgcgctc gacacggccg acgcggtcaa
     2701 ggcgaaaatg gccgagatga gccgcttttt ccctcccgga atgaaagtcg tttatccgaa
     2761 cgactccacg gtcttcatca aggtgtccat caatgaagtg atcaagacgc tcgtgatcgc
     2821 cattgtgctg gtgtttctgg tcatgctgct ttttctgggc agcatccgcg ccacgctgat
     2881 ccccaccatc gccgtgccgg tggttcttct cggaaccttc gccgtgctgg cgcttttcgg
     2941 ctttaccgtc aacatgctga cgatgttcgc catggtgctg gcgatcggcc tgctggtcga
     3001 tgacgcgatt gtcgtcgtcg aaaatgtgga gcgcatcatg acggaggaag ggctttcccc
     3061 caaggaagcc acggccaaat ccatggagca aatcaccagc gcgctggtcg gcatcggtct
     3121 ggtgctctcc gccgtcttcg gcccgatggc gttttttccc ggctccaccg gcatcatcta
     3181 ccgccagttc tccatcacga tcatgtcggc catgctgctt tcggtggccg tcgccctgat
     3241 tctgacgccc gtactgtgcg ccgggctcct caagcccgtc caggccggac atcagccggc
     3301 ggaaaacgcc gcctctttcg cccggccgtt tttcctgtgg tttgaccgcg tttttttcag
     3361 actgcgcgac cgttacgtcg gactggtcgg ccattcgctt tcccggaaaa aacgctacct
     3421 ggtcgtcttt ctggtcatcg tcgcggtcat gggcgttttg tttctgcgca tgcccagggc
     3481 ctacctgccg gatgaagatc agggaatcct gctggcacag gtcatcatgc ccaccggcgc
     3541 cacgctggaa caaaccggag aaatcacgac gcagatccaa aaatactttg aggaaaatga
     3601 aaaagaagcc gtggaatcgc tgatgacgat caccggcatc ggattttccg gacgctcgca
     3661 gaacaacggc atggtattta tccggctgaa ggactggcat ctgcgcgacc gggcggatct
     3721 gaaagcgaag gcgattgccg gccgggcgat gttcgccctt tccggtatcc gcaacgccat
     3781 ggtgtttacc ttccagcctc cgtccgtcat tgaactgggc acgtccatcg ggttcgattt
     3841 tcagctcctt gaccgcggag gggtggggca ttcggcactg acgaacgccc agtaccagct
     3901 tctcggcctc gcggcaaagg atccgcgggt tgccaaagtc cggcccaaca gcatggaaga
     3961 cgtcccggaa taccgcatcg acgtggactg ggaaagagcc ggcgcactgg gggttcccat
     4021 ctcatccatc cacaggacgc tgtccgccgc cttcggcagc gcctacgcca acgacttcat
     4081 tcagggcggg cgcatcaaac gagtgtatat tcaggccgac gccccgtacc gcatgctgcc
     4141 caaagatctg gaaaatctct atgtccgcaa tacgcagggc cgaatggtgc cttttgcatc
     4201 ctttgccacg ggccgctgga cgtcggggcc gccccggctc acgcgctaca acggctttcc
     4261 ttccatcaac atctggggag aaccggcccc gggacgcagt tcgggagaag ccatgcaggc
     4321 gatggaggag gccatggaaa aacttccttc cggcttcggc tttgactgga gcggcctttc
     4381 ctaccaggag cggatggccg gcacccaggc gccgctgctg tacgcctttt ccatttttgt
     4441 gatttttctg tgtctggccg ctctgtacga aagctggaca atccccattt cgattctgct
     4501 cgtgcttccg ctgggcgtca tcggcggcat tatcgcgtcc agcgcgcgcg ggctcgccaa
     4561 tgacgtgtat tttcagatcg gactcctcac cacactgggg ctgaccacca aaaacgccat
     4621 cctgatcgtc cagttcgcca agagcggtct ggagcgcggc atggggcttc tggaggcgac
     4681 cctggaaggg gcgaagctgc gctttcgtcc gatcgtgatg acgtcgctgg cctttggatt
     4741 cggcgtcctg ccgctcgcca tgactaccgg ggcgggcgcg ggcgcgcaaa acgccatcgg
     4801 caccagcgtg ctgggcggca tgatcacggc aaccctgctg gtggtgattt tttcgccgct
     4861 gttttacgtt ctgattgaaa cgttgttcgg cagaaagctg aaaacggacg cgggtaaaac
     4921 cggcgcgcct tctctttttg aacatttacg gcaaaccatt ttgaccggac gccggaagaa
     4981 aaaatcaagc cgtacggata aagcggatgc ttcagaggtt gagcaacatg aataaaaaat
     5041 ggcttttgat cctgatggtt cttttggcgg gctgcacgat ggcgccgaaa tacgagcgtc
     5101 cggaagcccc ggtgccgaac gagtggccgg caggccaatc ttatggggaa gagaaacagg
     5161 cattggagac gactcaaccg gggtggcgcg aatttatcac cgatcagcgc ctgcgggaaa
     5221 tcatcgccga ctctttgaaa aacaaccgtg atctgcgggt cgcggcgctg aacgtggaca
     5281 aggccagagc catgtacggc atcggacggg cctcgctgct gccttcgatc aacgcctcgg
     5341 gcgtctggta tcgggaaaga atcccggccg atctttcctc ttccggcaac tccgtcaccg
     5401 ccgaacaatt taacgtcagt cttggcatgg cttcctggga aatcgacttc ttcggacgca
     5461 tccgcagcct gaaagaccag gccctggaac aatacctggc cacggatcag gcaagccgca
     5521 gcgcgcagat tctgctggtg gccgcggtgg cgcaggttta ttggacgctg gcggcggacc
     5581 gggaggccct gagcctcgtt tcctccactc tttcggcgca ggaagcggcc tacaaaatga
     5641 tccgcaagcg tcatgaagtc ggactggcct cagagctgga tctgcgccgg gctcagaccc
     5701 aggtggatac ggccagaggg gatgtcgccc gctacacgca aatggtcgcg caggatgaaa
     5761 actacctgaa tcttctggtc ggctcgccgg tccaaaagga attattgccg ccgaatctgg
     5821 ccggcgtcag cccgccccgg gatatttcag ccggcctctc ttccgaaccg cttttgcggc
     5881 ggccggacgt tctggcggcg gagcaccggc tcaaagcggc caacgccaat atcggcgcgg
     5941 cccgcgcggc tctctttccc cggatttcgc tgacgacgtc cattggaacc gccagcgccg
     6001 aactgtccgg gcttttcaaa gccggctcgg acgtgtggac ctttgcgccg cagatcgtga
     6061 tgccgatttt cgatccccgc gtctggtatg cctacgacgt caccaaaatc gaaaaggaaa
     6121 tttccatcgc ccagtatgag aagtccattc agacggcctt tcgggaagtg gccgacgcgc
     6181 tggcggaaaa aggcatggtc aatgagcgca tcgcggcgca gcaatccctt gttgatgctc
     6241 tggccgtcgc ctaccgcctc tccgagctgc gctacgaaaa gggcatcgac agctacctct
     6301 ccgttctcga cgcgcagcgc tcgctgtacg gcgcgcagca gggcctgatc ctgctgcgtc
     6361 tggcttccat caacaatcgc gtggccctct acaaagcact gggcggaggc gcgtctccat
     6421 gacgaaaaag cggcgcccct gttcggcaaa ggaaatcaaa tattttctgt tgaatcgggt
     6481 tctgacgtcc gccattgttt tcgttttgcg gatttacgca aaaacgctcc gcgtgcggat
     6541 cgagggcaag gaagaaattc tgaagcatct ggccggcggg ggccgggtca tctttgccag
     6601 ttggcaccag cgccttttcg gaggtttttt tgttccgcgg ttttttcaat ggacgccctg
     6661 catcatgatc agccagagcc gcgacggaga cttcatttcc aaaatcgtct cccgcctcgg
     6721 ctggattccg gttcggggct ccagcacccg gggcggcgcg aaagcattga aagccatgcc
     6781 gcggacggcg gctggggaaa atgacgaaaa aacgcaaacc cgcaaaccta atttgacaaa
     6841 ttcaacctgg atctgacact caactgcgac ggggagccgc tctctctcac caactaccgg
     6901 tttattgggt tgctaaaaca aaatgtgcga aagattgttg acactacccg aaaacagaca
     6961 accgatgctg aaatgtcaag attaaagatt tgacccctta ccctaaagat ttgacccctt
     7021 acccccctta cccttttctc accggcaccg gctgaatcca ttttttgttg tcgccgtcgt
     7081 agtaccattt gtcgggaaag ccgcgtcggc ggatgaaggt gggatgatcg gcccagcgca
     7141 ggaggtcctg gtaggcggcg tgaagccggc gaaaggcggc ggccgtcccg ccggcatcgg
     7201 gatgatgaat tttcgcctgg cggcggtagg cgttgcggat gatcttatcc agatcgcggg
     7261 atgcgagatc cgctttggac atctccagaa gcagtatgct ttccggtttg acgcaaggcg
     7321 ccttcatcca ccgggggcgc ttgagtccgt cggccatggc cagtctttcg gccatctcca
     7381 gaacgcgcct tgaagccagg tagtttcgtg tggtcctctt ttcctcggcc caccaggcgt
     7441 cccccagcag attgctcatc ctgccgaaat catcggccgg ctttgtcccc gccgtgcgct
     7501 tcgtctggaa gcgaaacacc tcccgaatgc cgtgcggaac cacgtcgaga acaatcaacg
     7561 tatccgtaaa ataaaatgtt gcgtagcgcg tgttcagggc ctgcagcagt ccgcttcgcg
     7621 tattgagcct ctgccgcaga tgctggcggc atcggacgga gcagtagcgg cgcttcccga
     7681 tgttgccttc gccgcaggaa aggcaaacgc cctggggaaa atgatgggcg gatattttgc
     7741 ttttgaccag catgcgtgaa tcagccggcg ggttgaaaaa cctttcctcc ggccttgact
     7801 ttccttcaat gatttaccgg gccgccaggt atcccctggc cagcgccttg ttgacatgca
     7861 gaacgccttt gttcatctct ttcatcggat aatcggcggt gatcacggga
//
LOCUS       DAOSIM010000081         7875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_8538_length_7875_cov_7.251790, whole genome shotgun sequence.
ACCESSION   DAOSIM010000081 DAOSIM010000000
VERSION     DAOSIM010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8538_length_7875_cov_7.251790"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(344..631)
                     /locus_tag="PLR47_04940"
     CDS             complement(344..631)
                     /locus_tag="PLR47_04940"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOG12321.1"
                     /translation="MKVYWTENAIRHLFNIYEYIAANSPTYALRMVDRITRRSEQIAE
                     HPLSGRTVPEYEAEYIREIIEKPYRIIYRIKPEQIDVLAVIHGAQILPENV"
     gene            complement(628..816)
                     /locus_tag="PLR47_04945"
     CDS             complement(628..816)
                     /locus_tag="PLR47_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12322.1"
                     /translation="MQTANVKQEASRLLNNLPEDATWDDLMYRIYVHQTIEAGLKDSD
                     AGHTVDVKEVRKRFGLAK"
     gene            complement(834..1220)
                     /locus_tag="PLR47_04950"
     CDS             complement(834..1220)
                     /locus_tag="PLR47_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG12323.1"
                     /translation="MPRSKAQVNPNEEPVGAQLARLRQAAGFSQRDLAKETGISQRMI
                     AYYEKQPQYPPLHVLTILTKALGVPPGEMLGLKIKKTDDKVKDMRIRRRLKQVEGLDE
                     KEKRQILQLLDTFIENNRLKQKSISA"
     gene            1569..1880
                     /locus_tag="PLR47_04955"
     CDS             1569..1880
                     /locus_tag="PLR47_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12324.1"
                     /translation="MTAQSVETTDIFRSAFFLCHGGNLTGINIKNNGRRIATFVISGD
                     DLERLDRQYRNGEALVNPVQFRESLTHLRDTLFDRLRHYDTGDNRYDRKRANRGHQTG
                     Y"
     gene            1837..4551
                     /locus_tag="PLR47_04960"
     CDS             1837..4551
                     /locus_tag="PLR47_04960"
                     /inference="COORDINATES: protein motif:HMM:NF019880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HOG12325.1"
                     /translation="MIEKERIEAIKRDIDLAAFIESRGIMLKKNGKGYTGLCPFHEDT
                     NPSLSVNPSKNLWQCFGCGTGGDIIRFVELFDKVSFPEAVKQLSVAGSQLPGKTLTKA
                     NHQPKTGNGQPSTNLTIKEQKLLARVVGYYQHTLTQDQQGLRYLKEERGITDNPSIND
                     FGTGFVNGTLPEILPDDEEAITSLKKIGILNAKGHETFYNCIVFPLYDNNGGIVNLYG
                     RNITEDNPSTSSGRVTHLYLPGARSGIINRQAVKRSQTILLTESVIDALTLYAQGFKN
                     VIPIYGVNGLLDEHMALFKGRIKESFIIFDADEAGIKGAEALALRLKEKGILSYIVTL
                     PVKDVNIFFKRQTPEELEALLKKANPQALEQSEKISKREQSLYQETEHGFIVGYGNRY
                     YEIKGIQRSDTQLKATVKASKDMSVQSKMPFELSTIDLYSSRSRQWFAKATSDIFGAS
                     DELIKEDLGKLMTLAESYRLKDKKANVTEATEEEKNEAARFLKNPDLFDEILDDLETI
                     GVTGEETNKLVGYLAAVSRKLDEPLSILIQSRSAAGKSTLQDAVLSLVPEEDYVKYTR
                     MTDQALFYKAEDSLVNKIIAIEEEEGMGGAAYSIRNIQSAKKITVAATGKDTTTGKMK
                     TEEYTVRGPVAVMITTTAAELEGETASRFLFLTIDESTKMTEAIHNKQREADTLEGLI
                     KKNKKEKIIKKHHTAQRLLKPLAVVNPFSQYLSYPTGSLRTRRDHKKYLNLIRAIAFL
                     FQSQREIKTINPFDDAQGTAVEYIEVTLDDIDKANKLANEVLGQSMDELAKPSRTLLS
                     LIYQMVKEETEKGKLSLDDFFFNRRMIREYINWTDWQIKAHIKQLEEMEYLHVRMGAQ
                     GKQYSYALNYKGQGEENGKCFLNLTPVEEIRKLMKGEASEKAKPETQH"
     gene            4714..5619
                     /locus_tag="PLR47_04965"
     CDS             4714..5619
                     /locus_tag="PLR47_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOG12326.1"
                     /translation="MDVVVTIVEYKNQLKARGYAPASIESYGKGLDLFSRYLQAQGID
                     DLRKVTKQTLFDYQSKIMEETIAQESKALKIRPVKRLFEYLIENNHLLINPAEGIVET
                     NRTNRKIGSTLTLAEMQKLLQTPNLSVRTGIRDKAVMELLYCTGIRRDELLNLDIYDA
                     DLKDHALYIRKAKGRKQRVVPLGNTASKYLKEYLEKIRPHHAKKNPKERRLFLSTSGL
                     PLTAGALHAAIRAYRLIAGIKKPVSPHTFRRSCATHLMQQGVDVRYIQKLLGHSSLKT
                     TQVYTKVMPVDVKKTHERTHPNIGE"
     gene            5665..6606
                     /locus_tag="PLR47_04970"
     CDS             5665..6606
                     /locus_tag="PLR47_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOG12327.1"
                     /translation="MTIKELIPVYLAHLRITGRAERTIKNTRYSLNDFVRFLDGEEIL
                     NIEDITAEVMLAYQEDLAFRLTAGGKLLAIRSQDKPLSVARGFARYAHEQDYLVSDPG
                     KKIKPPRKPKTLPRGIMSQQEVKKVIDATDMHESRGYRNRIVLEILYDTAIRRQEICN
                     IKISDIDLDEGYVHIRGGKGNKDRVTPVSKRVCDLIGNYILAVRPAFIKGKDDGYLIL
                     NRWGQKMDATGMGIWSIVKRYVRLAGIDKNVSPHTFRHTCATHMLKNGAPVRHIQEML
                     GHESLESTQIYTRVTINDLKEIHAKYHPSETMNKKER"
BASE COUNT         2265 a         1929 c         1945 g         1736 t
ORIGIN      
        1 ttaccagatt gagggctact tctcaaaatg tgcgaaaaat attatacgtt atcaaggaaa
       61 cataacttgg ttatgtttag cgtgcgaggg aaggttcaag ggcgggacgc ctgctttctt
      121 ttcattgcag acggtgtgcc cttgaagcca tgtctgataa ggctgcctta tcagacgtgc
      181 cgagcaaagc gacttcccgc aaacgccctt gcaacaacca ccgggcgaca ttgacacgaa
      241 gcccagcgcc ggggcgggcg gggaataaat aatagacagg ctgcgagtcg caggcagcga
      301 aggccacagc gtggccgcag cccgagcacg cagacgcacc ctcctataca ttctccggta
      361 aaatctgcgc cccgtgaatg actgccagaa cgtcaatctg ttcgggtttg atcctgtaaa
      421 tgatccggta gggtttctcg ataatctcgc ggatgtattc cgcctcgtat tccggcactg
      481 tccggccgga caagggatgc tcggcgattt gctcggaacg gcgggtaatc ctgtccacca
      541 tcctcaaagc ataggtcgga gaatttgcgg cgatgtattc gtaaatgtta aaaagatgcc
      601 ggatggcatt ttccgtccag taaactttca tttagcaagt ccgaaccttt tgcggacttc
      661 tttgacatcg accgtatgac cggcgtcgct gtctttcagc ccggcttcaa tggtctggtg
      721 cacatagatg cgatacatga gatcgtccca tgtcgcatct tccggcaagt tgttcaataa
      781 ccggctggct tcctgtttta cgtttgctgt ttgcatctgt tgttctcctt gtattaggcc
      841 gaaatggatt tttgtttcag ccggttgttt tcgatgaaag tatccagcag ttgcagaatc
      901 tggcgttttt ctttttcgtc cagaccttcc acctgtttga gccttcgccg gatacgcata
      961 tctttcacct tatcgtctgt ctttttaatt ttcaagccga gcatttcccc cgggggaacc
     1021 cccagcgctt tggtcagaat ggttaataca tgcaacggcg gatattgggg ctgtttctca
     1081 taataggcga tcatccgttg ggaaatgccg gtttctttcg ccaaatcgcg ttgtgaaaat
     1141 ccggcggcct gacgcagacg tgccagctgc gctccgaccg gttcttcgtt ggggtttact
     1201 tgtgccttgc ttctgggcat gacggcgctc ctttttgtaa agtcacgcct attatacacg
     1261 atgattttgg acgccatacg atacccttca aataatgatt gacatgccga tacatttaat
     1321 gtatcacatg cctgacaatt ttagaataaa tatttttggc attttaatga aagaaatcgc
     1381 ttgacagtga tctggcgtgg gtttcaggag gtaagaggcc gtcaaccatc ggactttggc
     1441 gagtttccgg ttgacggcct ggaaaaaact gaagacgggc aagcccgtcc ggttttgtct
     1501 tgcctaacgt aaatcccccg gaaaatcaag gcggcatccc aacaacagac agaaaggagg
     1561 tgaatacatt gacggctcaa agcgtagaga cgacggatat ttttagaagc gctttctttt
     1621 tatgccacgg cggcaacctg acaggaatta acatcaagaa taacggcaga aggatcgcca
     1681 cgtttgtcat cagcggcgac gacctggagc gcttggacag acagtaccgc aacggcgaag
     1741 cattggttaa tcccgtgcag tttcgggaat cgctgacgca tttaagagac accttgtttg
     1801 acagattaag acactacgac acgggggaca atagatatga tcgaaaaaga gcgaatcgag
     1861 gccatcaaac gggatattga cctggcggcg tttatcgaat cccgaggcat tatgctaaag
     1921 aaaaacggca agggatacac aggcctttgc cccttccatg aagacaccaa cccatcacta
     1981 tcggtaaatc catcaaagaa cctctggcag tgtttcggct gcggcaccgg cggggatatc
     2041 atccggtttg tggagctgtt cgacaaagta tcctttccgg aggcggtgaa acagttatcg
     2101 gttgccggtt cacagttgcc gggaaagacc ttgacgaaag cgaaccacca accgaaaacc
     2161 ggaaacggtc aaccgtcaac gaatttaacc atcaaagaac aaaagcttct ggcccgtgtt
     2221 gttggttact accagcacac cctgacacag gatcaacagg gtttaagata cctgaaagag
     2281 gaaagaggca tcaccgataa tccgtcgatt aatgacttcg gcaccggctt tgtcaacggc
     2341 accctgccgg agattttgcc ggacgatgaa gaagcaatca cgtccttaaa gaaaatcggc
     2401 atcctgaatg ccaaaggcca tgaaactttt tacaactgca tagtgtttcc gctttatgac
     2461 aataacggcg gcattgtgaa tctttatggc cgcaatatca cagaggataa cccttcgaca
     2521 agctcagggc gagtcacgca tctttaccta cccggcgcaa gaagcggcat catcaaccgg
     2581 caggccgtga aacgttcaca aaccatcctc ttaaccgaat ccgtcattga cgccttgacc
     2641 ctgtatgccc agggcttcaa gaatgtaatc ccgatctatg gcgtcaatgg cttgctcgat
     2701 gaacacatgg ccttgtttaa gggcagaata aaagaatcct tcatcatctt tgacgccgat
     2761 gaagcaggca tcaaaggcgc tgaagctctg gccttgcggt taaaggaaaa agggatttta
     2821 tcttatatcg tcactctgcc ggtgaaggac gtcaatatct tctttaaacg ccagacaccg
     2881 gaagagctgg aagcactgct caagaaagcc aatccgcagg ccctggagca atcggagaag
     2941 atcagcaaac gggaacagag tttgtatcag gaaaccgagc atggctttat cgtaggctat
     3001 ggcaaccgct attacgaaat caagggcatc cagcggtcgg acacgcaact gaaagccacc
     3061 gtcaaagcct ccaaagacat gagcgtccaa agcaaaatgc cctttgaact ttccaccatc
     3121 gatttatact catcacgatc aagacaatgg tttgccaaag ccacctccga catctttgga
     3181 gcatctgacg aactgatcaa agaggattta ggcaagctga tgacgctggc cgaatcctat
     3241 cgactcaaag acaagaaagc caatgttacc gaagccacgg aagaagaaaa gaatgaagcc
     3301 gcccgctttc tgaagaatcc cgaccttttt gacgaaatcc tcgacgatct ggaaaccatc
     3361 ggtgtcacgg gcgaagaaac caacaagctt gtcggatatc tggctgccgt atcccgaaag
     3421 ctcgacgagc ctttatccat cctgattcaa tcccgatccg ccgcgggcaa atcaacgctt
     3481 caggacgccg tcttgtcgct ggttccggaa gaagactatg tcaaatacac ccggatgacc
     3541 gaccaggccc tattttataa agccgaagac tcactggtca acaagatcat cgccatcgag
     3601 gaagaagaag gcatgggcgg agccgcttac tctatccgga acatccagtc cgccaaaaag
     3661 atcaccgtcg ccgccacggg caaagacaca accaccggca aaatgaaaac ggaagaatac
     3721 accgtccgcg gcccggtggc cgtcatgatc acaacaacag cggcggaatt agaaggcgaa
     3781 acagccagcc gctttttgtt tctcaccatt gatgaatcaa ccaagatgac ggaggcaatc
     3841 cataacaagc aaagagaagc cgacacgctg gaaggattaa tcaagaagaa caaaaaggaa
     3901 aagatcatca aaaagcacca caccgcccag aggcttttaa aacctttggc cgtggtcaac
     3961 cccttcagtc agtacctttc gtatcccaca ggctcattac gaacgagaag agaccataaa
     4021 aaatacctca acctgataag agccattgcc tttttgttcc agtcccaaag agaaatcaaa
     4081 acaattaacc ccttcgacga tgctcagggc acggccgtgg aatacatcga agtgacgctg
     4141 gacgatatcg acaaggccaa caaactggcc aatgaggttt taggccagag catggacgaa
     4201 ctggccaagc cgtcccggac gcttttgtcc ctgatttatc aaatggttaa agaggaaacg
     4261 gaaaaaggaa aactctcttt ggatgatttt ttctttaatc gccggatgat ccgggaatac
     4321 atcaactgga cggactggca gatcaaagcc catatcaagc aactcgaaga gatggaatac
     4381 ctgcatgtcc gcatgggcgc acaagggaaa caatacagct atgctcttaa ttacaaaggc
     4441 cagggagaag aaaacggcaa atgctttttg aatctgaccc ccgtggaaga aatcagaaaa
     4501 ctgatgaaag gcgaagcgtc ggaaaaagca aagcctgaaa cgcagcattg acggtacttg
     4561 gtgggcaatc acccagacta gtagggtaaa aagacagctt ggagggtact tggaggtttt
     4621 agaaaaacaa actaaccgcc tgcaattaaa gaaaaaagaa aactagttgg aggtgaaggc
     4681 gaaagaaaga cccaggggga gagagggggc aatatggatg tcgtcgtaac catagtagaa
     4741 tacaaaaacc agcttaaagc ccgaggctat gccccggcgt caatagagag ctacggcaaa
     4801 ggcctggacc tgtttagccg ttatctgcaa gcccagggca tcgacgattt acgcaaggtt
     4861 acgaaacaaa ccctcttcga ctatcaaagc aagatcatgg aagaaaccat tgcccaggag
     4921 agcaaagccc tcaaaataag gccggtgaag cgcctgtttg aatacctcat cgaaaacaat
     4981 cacctgctga tcaaccccgc agaaggcatc gtggagacca acaggacaaa cagaaagatc
     5041 ggttcaactc tgacccttgc cgaaatgcaa aagcttcttc aaacgcccaa tctgtccgta
     5101 agaaccggca tccgggacaa agccgtcatg gagctgcttt attgcaccgg aattcgaaga
     5161 gacgagcttt tgaatctgga catctacgac gccgatttaa aagaccatgc cctttacatc
     5221 cgcaaagcca agggaagaaa acaaagggtt gttccgctgg gaaacacggc gtcaaagtat
     5281 ctgaaagaat acctggaaaa gatcaggccg catcatgcga aaaagaaccc gaaggagaga
     5341 agactctttt taagcacatc cggcttgccg ctgacagccg gggcgctcca tgccgcaatc
     5401 agagcgtaca ggctgattgc cgggatcaag aagccggtat ctccgcacac tttccgcagg
     5461 agttgtgcca cgcatttaat gcagcaaggg gtggatgtgc gttacatcca gaagctgtta
     5521 ggtcatagca gtctcaagac cacgcaggtt tacaccaaag tcatgccggt ggacgtcaaa
     5581 aagacgcatg aaagaacgca tccaaacata ggtgagtagg ctgtagactg ttagactatt
     5641 aggcaaagaa gaaaggcata gagcatgacg attaaagagt tgattcctgt ttatctggcg
     5701 catctccgaa tcacggggcg ggcggaaagg acgattaaga acaccagata ctcgctcaat
     5761 gattttgtgc ggttcctgga cggagaagaa atcctgaaca ttgaagacat caccgccgaa
     5821 gtgatgctgg cctatcagga agacctggcc ttccgcctga cggccggagg caagcttttg
     5881 gcgatccgtt cgcaggacaa gccgttatcc gttgcccgtg gctttgcccg gtatgcccat
     5941 gaacaggatt accttgtttc cgatccgggg aaaaagatca aaccgcccag aaagcccaag
     6001 accctgccca gaggaatcat gagccaacag gaagtaaaaa aggtcatcga tgcaacggac
     6061 atgcatgaaa gccggggata ccgcaaccgg atcgttttgg aaatcctcta tgatacggcc
     6121 atccgccggc aggaaatctg caacatcaaa atatccgaca tcgatcttga cgaagggtat
     6181 gtccacatcc ggggcggcaa ggggaacaaa gacagagtga cgcccgtcag caaacgggtt
     6241 tgcgacctga tcggaaacta tatcctggcc gtccgaccgg cttttatcaa aggcaaggac
     6301 gacggttacc tgattttaaa ccgctggggg caaaagatgg atgcaacggg catgggcatc
     6361 tggtccatcg tcaagcgtta tgtccgcctg gcggggatcg acaagaacgt ttcgccgcat
     6421 accttccgcc acacctgcgc cacgcacatg ctgaaaaacg gcgctccggt gcgtcacatt
     6481 caggaaatgc tgggtcatga gtctttggaa tccacgcaga tttacacaag agttacgatc
     6541 aacgatctga aggaaatcca cgccaagtat catccgtcgg aaacgatgaa caaaaaagaa
     6601 aggtaacgac tgattctatg gagtcgaggc ggtgtgtctg cgtgctcggg ctccagccgc
     6661 ctgcggcgtc ttgcgcttcc tgcactcgca gccacgattt gattttattc ccccgcccgc
     6721 cccgcaaaag agcgccgttc aagggccgct gcgctctgcg ggaagtcgcc gcgttcggcc
     6781 cgtcagataa tgcgcattat cggacatggc ttcaaggtca tgccgcttgc agtaaaaaag
     6841 caagcgtccc gcccttgaac cttccctcac gccgctaaac ataacaagtt atgtttcctt
     6901 cccgcaaagc tccgctagac ttaaaggtgc ggcgctcttt tgctcccgtc cgcccccagg
     6961 ggctcccgga aaaatagaat ttaaaaaaag aaaaacgcga ttaaagaaaa aacagcaaaa
     7021 agcttttttc tttaactgca atatctgctt gatttatgaa ggccatctct ttaagctgac
     7081 ggcggttctt tgaaatgagg atgcataggg gcaaaaaaag aagctcttat ggagggaggc
     7141 ccatcgaact ctctacggca agggaatatg acggagaaaa ggggctttat tactaccggg
     7201 caagatatta tgatccgaag gcagggaggt ttattacaag ggatccgatc tcgtttgccg
     7261 gagggggatg ttaacctgta tgcgtatgtc gggaataatc cggttaactg ggcggatccg
     7321 gatggtttgg cgactaagcc aggagagtgc cccaattagt aaattcagag tataaattca
     7381 gggtataaat tcagaggaca taacacttaa ttaatcgcaa catgttttta tgacggcggt
     7441 tgaagttttt tttcaaccac cgtcgcctga caaatacatt aaatgaaaac tctacctgtt
     7501 agcagttgta atgagcatgt gccgtatttg ggacatgttc aacaatgatc gttgtagttg
     7561 attaaaaaat tcaaatattt tctggcattt acaaatgttg caaaattaag gcgatatttc
     7621 ctcttcagaa accaagatat tcttgacatt gggtcatgat ttcctataca aaacataccg
     7681 acgcaatttt gtatcaaata aaaggatgcg tctgatgtgg atgtagcagt atcggcaaca
     7741 ctgaagaaat gtaaagtttc atcaagagga aagctttgca taaccaggtc catttattta
     7801 ttctgtcata ccggcgtagg agactgtgtc gtaatgtcat tgcgaaggag caacagcgac
     7861 tgaagcaatc tccta
//
LOCUS       DAOSIM010000082         7872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_8541_length_7872_cov_7.020340, whole genome shotgun sequence.
ACCESSION   DAOSIM010000082 DAOSIM010000000
VERSION     DAOSIM010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7872
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8541_length_7872_cov_7.020340"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PLR47_04975"
     CDS             <1..835
                     /locus_tag="PLR47_04975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG12328.1"
                     /translation="LKRVRPLAEKNAVSQKDLDDAVSAEKSARAAVFAAQGSVQDAEL
                     KLSYTTITSPIDGLAGRAKQQEGSYISVGQASLLTYVAKIDPIWVDFSISENENLKLM
                     DHLAKGLFKMPKGSDFEVKVQMADGSIFPHKGRLNFADPSYNSETGTYEVRAEIPNPI
                     NSPTVIRPGQFVRVHLLGGIRPNGILIPRTAVNQGAQGFFVWIAGKDGKAEFRAVEVG
                     DWQGNDVFINSGLAAGDRIILDNLLKMSPGAPLKIDTSPVAKGAAPVTDQKSPAPPVK
                     K"
     gene            849..4031
                     /locus_tag="PLR47_04980"
     CDS             849..4031
                     /locus_tag="PLR47_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HOG12329.1"
                     /translation="MISHFFIDRPVFASVISIVIVIAGLVAMSNLPIAQYPDITPPVV
                     TVSATYPGASADVISENIAAPIELQVNGADNMIYMSSTSSSSGMMNLSVYFEIGTSPD
                     LAQVDIQNRVNQALPQLPQSVQQQGVIVSKKSSSFLMVVGIYSPNDRYDHQYVANYAN
                     LYILDAIKRIPGASQAAIMGMPDYAMRIWLKPDRLIQLGITASDVANAIKNQNQQFAV
                     GKIGQAPTKGRVEQTFSVTTKGRLIEPAEFENIILRVNAQGAAMVRLKDVGRAEFGAK
                     DYGTRCRLNGKDATIIAVFQQAGANSLDVSKQVEKTLKELKKGFPDGIDYKIAMDTTK
                     FVRASIKEVVITFFEACLLVIVVVFLFLQSIRATVIPILAIPVSIIGTFIGMTLFGFS
                     INMLTLFGMVLAIGIVVDDAIVVIENVERNMREFNLSPKDAAKKAMEEVTGPVVAIVL
                     VLCAVFIPVAFLGGITGQLYKQFAITIAMSVVFSGIVALTLSPAMAALLLKPRHGEKN
                     RFFRTFDAKFAGLTEGYSRGVSLIIKRVAVALCIFAVMLTCVFVLFRHVPTSFVPSED
                     QGYLLGVALLPDAASLDRTQDLNQKVRTIYAGHPAVENVVEVDGYSLLDGESKTNAGL
                     QFISLNDYEKRKPKELRAPAIIDKTSKLFLGLREGFVMPMNPPSIPGLGVTAGFEFWI
                     QNRGSGDMSKLESLTKELVARAEKQPELQGVATLFNVHSRQLLVDVDREKSENLGVPV
                     EDVYNTLQILFGSSYVSQFTKFSRLWQVILQAEPEYRSRPSDMDQIYVRSRSGSMVPL
                     KALVSQKNVTGPDLVTRFNNFPAAKVIGGPAPGYSSGDAIAAMERVAAEVLPQDFSYA
                     WSGESFEEKKSGGTTVVVFIFGLIMVFLILAAQYEKWSLPFGVLMAVPFAIFGALVAI
                     WLRGQENDIYFQIGLITLIALAAKNAILIIEFAVLKRQEGMSAFDAAVSASKMRLRPI
                     VMTSLAFILGCVPLAVASGASANSRHSIGTGVIGGMLGATLIAVFFIPMFYFLLEKLT
                     ERLGRSGNKKLQSGAAPTATTGETPAGEGK"
     gene            4033..5460
                     /locus_tag="PLR47_04985"
     CDS             4033..5460
                     /locus_tag="PLR47_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOG12330.1"
                     /translation="MIRKISILFFILLIAGCAVGPDYRRPDIQAPTEWRFEDKEARTL
                     INARWWEQFNDPVLNGLIETALKENKDVLIAAARIEEYEGRYAAARGDLFPQAQAAGS
                     ATRQRSTEAGASPWPAGVSNPYPAYQTTLNASWEIDLWGKLRRGTEAARADLLSRENA
                     HRAVLLSLTANVANAYINLRDYDRQLEIAKETLKTREASLEIYELRFDAGVISDLELS
                     QMRSEYENTRATIPQIEKSIHQQENALNLLLARNPGPVVRGLTIDQLNFPHVPEGLPS
                     ELLERRPDIRQAEQDLIAANARIGVARAAYFPAISLTGTLGSASADLSNLFTGPAYIW
                     NAGASVTVPIFTAGRTWGQVKASEALQKQALLTYQKTIQTAFREVEDSLVDQNRTRAK
                     LEALFNQLTALRNYRNLALLRYENGYSSNLEVLDAERNLFSIEITYIETRGGLFNAFI
                     SLYKAMGGGWGVDGSPPPKAPAAEK"
     gene            5488..6573
                     /locus_tag="PLR47_04990"
     CDS             5488..6573
                     /locus_tag="PLR47_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOG12331.1"
                     /translation="MAKSRNRKTLTALSLQGGGALGAYEYGVLKALYEKRGKGFTPKV
                     VAGVSIGAINAALLVGAKGDPLTALDRLWRERLCVSWPSLPDCSWAGLTPWSANSLAE
                     RNLSFFANSGMYCLKPEFFLMPALAWLKNSSLYDTSFLRETLADFVDPEKLNRPEKTR
                     LIVTAVNVKTGQLSRFDNSRMELTFDHIIASGSFPVTFPMTQIGEDFFWDGGVFMNMP
                     VGEAVNALEQIEADNPDVEREVIVIELHRMQADLPKTIPEAAERFCNLIFSGKFALDR
                     KLYDRYGAFVDLMGEIDRTIPADSPIRSHKGYKMMTRHRKIDRAMVIGEEGTGATGSG
                     GDFSRNALNRRIEAGYGDAMAFFKKNR"
     gene            complement(6628..7212)
                     /locus_tag="PLR47_04995"
     CDS             complement(6628..7212)
                     /locus_tag="PLR47_04995"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOG12332.1"
                     /translation="MISRIDHVSIAVRDQEKEKAEHFFRDILGAVAGAGMADPVSRFF
                     WQIYSLGDLSRLEIISPTEPGSFLDGFLSQRQAGVHHITLQTPDIRKAMAHLEAHGIP
                     YFGFNEYPGGVWKEIFIHPRHGFGVLIQIAEFEADDWLAEQVRFPAGTKWQVEKTENG
                     AALIFAHPGGGRVSLELGREDLTRLAKEIRELPG"
     gene            complement(7338..>7872)
                     /locus_tag="PLR47_05000"
     CDS             complement(7338..>7872)
                     /locus_tag="PLR47_05000"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOG12333.1"
                     /translation="SYSFAQCYNGYVDIGFLGFAQVDMYGNINTHMIGKDYHHPKVRL
                     TGSGGNNDLSSLTEKMVLVGLQTPDKFPEKVDFATSVGWLKGGDSRKEAGLIGNGPAA
                     VISPWGVYDFHPVSKRMRVKSLTPGVNFELAQQLTGFELLKPEGAIPETKLITPDCLK
                     VIRTEVDPRGVFTTMPS"
BASE COUNT         1701 a         2408 c         2150 g         1613 t
ORIGIN      
        1 gctgaaaaga gtccgtcccc tcgcggagaa aaacgccgtc agccagaaag acctcgatga
       61 cgccgtcagc gcggaaaaga gcgcccgcgc cgccgtcttt gccgcccagg gaagcgtcca
      121 ggacgcggag ctgaaactga gctacaccac gattacctcc cccattgacg gattggccgg
      181 acgggcaaag cagcaggagg gaagctacat ctccgtgggt caggccagcc ttctgaccta
      241 tgtggccaag atcgacccca tctgggtgga tttcagcatc tcggaaaacg aaaacctcaa
      301 gctgatggac catctggcca aagggctttt taaaatgccc aagggctccg atttcgaggt
      361 caaggttcaa atggccgacg gctcgatctt cccccacaag ggccgcctta atttcgccga
      421 tccttcctat aattccgaaa ccggaaccta tgaggttcgg gcggaaattc ccaatcccat
      481 caactccccg acggtcatcc ggcccgggca gttcgtccgc gtgcacctgc tgggaggcat
      541 ccgccccaac ggcatcctga tcccccgaac ggccgtcaac cagggcgcgc agggattttt
      601 cgtctggatc gcggggaaag acggcaaggc ggaattccgc gccgtggaag tcggagactg
      661 gcagggcaat gatgtgttca tcaacagcgg ccttgccgcc ggcgaccgca tcatcctcga
      721 caacctgctc aagatgtcgc cgggagcacc cctcaagatc gatacgtccc cggttgccaa
      781 aggcgccgca cctgtcacgg atcagaaatc gccggcgccg ccggtgaaga aataggagaa
      841 aattataaat gatttcccac ttcttcatcg accgccctgt ttttgcctcc gtgatttcca
      901 tcgttattgt tatcgccggg ctggtggcca tgagcaatct gcccatcgcc caatacccgg
      961 atatcacacc tcccgtggtc accgtctcgg caacctatcc gggcgccagc gccgacgtca
     1021 tctccgaaaa tatcgccgcc cccatcgaac ttcaagtgaa cggggcggat aatatgatct
     1081 atatgtcctc cacaagttcc agctcgggta tgatgaacct cagcgtctat ttcgaaatcg
     1141 gcaccagccc agatctggcc caggtggaca ttcaaaaccg cgtcaatcag gccttgcctc
     1201 agcttcccca atccgtccag caacagggcg tcatcgtcag caagaaatcg agcagcttcc
     1261 ttatggtcgt cggcatctat tcgcccaacg accgctatga ccaccagtat gtcgccaatt
     1321 atgccaatct gtatattctc gacgccatca agcgaatccc cggcgccagt caggcggcga
     1381 tcatgggcat gcccgactat gccatgcgca tctggctgaa gcccgaccgg ctcattcagc
     1441 tggggatcac agcctccgac gtggccaatg ccatcaaaaa ccagaatcag cagttcgccg
     1501 tcggcaagat cggccaggcc ccgacgaagg ggcgcgtaga gcagaccttc tccgtgacga
     1561 ccaagggaag gctcatcgaa cccgccgaat ttgaaaatat catcctccgg gtaaacgctc
     1621 aaggcgccgc catggttcgc ctcaaagacg tcgggcgggc ggaatttggg gccaaagact
     1681 atggcacccg ctgccgttta aatggaaaag acgcaacgat tattgccgtt tttcagcagg
     1741 ccggcgccaa ctccctggac gtttctaaac aggtggaaaa aaccctgaag gagctgaaga
     1801 aaggctttcc cgacggcatt gattacaaaa tcgccatgga caccacgaaa tttgtgcggg
     1861 cctccatcaa agaggttgtc atcactttct tcgaggcctg cctgctcgtc atcgtggtcg
     1921 tcttcctgtt tctccagagt atccgcgcca cggtcatccc cattctggcc atccccgtct
     1981 cgattatcgg aacctttatc ggcatgacgc tctttggatt ttccatcaac atgctgacgc
     2041 ttttcggaat ggtgctggcc atcggcattg tcgtcgatga cgccatcgtc gtcatcgaaa
     2101 atgtcgagag gaacatgagg gaattcaatc tatcgcccaa ggacgcggcc aagaaagcca
     2161 tggaggaggt cacggggccc gtcgtcgcca tcgtcctcgt tctgtgcgcc gttttcatcc
     2221 ccgtcgcttt tctcggcggc attacggggc agctctacaa gcagtttgcc attaccatcg
     2281 ccatgtccgt ggttttttcc ggaattgtcg ccctgacgct ttcccccgcc atggccgctc
     2341 tgctcctgaa accccggcac ggggagaaaa accggttctt caggacgttc gatgcgaaat
     2401 tcgccggtct gacggaagga tactccaggg gcgtctcgct catcatcaag cgtgtggcag
     2461 tggccctgtg catctttgcc gttatgctca cctgcgtctt cgttctcttt agacatgtcc
     2521 ccaccagctt tgtgccctcg gaagaccagg gctacctcct cggcgtcgct ttactgcccg
     2581 acgcggccag tctggaccgc acccaggatt taaatcaaaa ggtcagaacg atttatgccg
     2641 ggcatccagc cgttgagaac gtcgtggaag tggacggcta cagcctcctg gacggagaaa
     2701 gcaaaaccaa tgcgggtttg cagttcattt ccctcaatga ctacgagaaa cgaaaaccca
     2761 aagagctccg cgctccggcg atcatcgaca aaacatccaa attgtttttg ggacttcggg
     2821 aaggattcgt catgccgatg aacccgccgt cgatccccgg cctgggagtt accgccgggt
     2881 ttgagttctg gatacagaat cgcgggtccg gggacatgtc caaactggaa tccctcacaa
     2941 aagaattggt cgcccgggcg gaaaagcagc ccgaactcca gggtgtagcg acccttttta
     3001 acgtccattc ccggcagctt ctggtggatg tggaccggga aaagtcggaa aatctgggcg
     3061 ttcccgtgga agacgtttac aacacgctcc agatcctctt cggttcctcc tacgtcagtc
     3121 agttcacgaa attcagccgc ctgtggcagg tgatcctcca ggcggagccg gaataccgct
     3181 cccgtcccag cgatatggat cagatctatg tccgatccag aagcggcagt atggtgccct
     3241 tgaaagccct ggtctcgcag aaaaatgtca ccggccccga tctggttacg cggtttaata
     3301 atttccccgc cgccaaggtt attggcggcc ccgcccccgg ttacagttcg ggagacgcca
     3361 tcgcagccat ggagcgagtc gcggcggaag ttcttcctca ggacttctcc tacgcatgga
     3421 gcggagagtc ctttgaagaa aagaaatcgg gcgggacgac ggtagtggtc tttatcttcg
     3481 gcctgatcat ggtgtttctc attctggccg cccagtatga aaaatggtcg ctgcccttcg
     3541 gcgtcctgat ggccgtacct ttcgccatct tcggcgcgct cgtcgccatc tggcttcggg
     3601 gccaggaaaa tgacatctat ttccagatcg gtctgatcac gctgattgct ctggctgcaa
     3661 aaaacgccat tctcatcatc gaatttgccg tactgaagcg ccaggagggg atgagcgcct
     3721 ttgatgcagc ggtttcggcc tcgaaaatgc gcctgcgccc catcgtcatg acctctctgg
     3781 cctttattct cggctgcgtc cccctggccg tcgccagcgg cgcctcggcc aacagccggc
     3841 actccatcgg cacgggcgtc atcggaggaa tgctgggcgc gacactgatt gccgttttct
     3901 tcattccgat gttctacttc cttctggaaa aactgacgga gcgtttggga agatcgggaa
     3961 acaaaaaatt gcagagcggc gccgcgccaa cggcaacaac cggggaaacc ccggccgggg
     4021 aggggaaata aaatgatcag gaagatatcc attctctttt tcatcctcct catcgccggc
     4081 tgcgccgtcg gtccggatta ccggcgtccg gacatccagg cgccgaccga atggcgcttt
     4141 gaggacaagg aggcgcgaac gctgattaat gcgcgctggt gggagcagtt caacgatccc
     4201 gtcctcaacg ggctcattga aacagccctg aaggagaaca aagacgtcct catcgcggcg
     4261 gcgcggatcg aggagtatga gggccgctat gcggctgccc ggggcgatct ctttccccag
     4321 gcgcaagcgg cagggtccgc cacaaggcag cgttccacgg aagcaggagc gtcgccctgg
     4381 cccgccggcg tttccaatcc ttatcccgcc tatcagacca cgctcaacgc cagttgggaa
     4441 attgatctct ggggaaagct gcggcgcggg accgaagcgg cccgggcgga tctcctttcc
     4501 cgggagaatg cccaccgcgc cgtgcttttg tccctcacgg caaatgtcgc caacgcctac
     4561 atcaacctgc gggattatga ccgccagctc gaaattgcga aagaaacact taaaacccgt
     4621 gaagcatccc tggaaattta tgaactgcgc ttcgacgccg gcgtcatttc ggatctggag
     4681 ttaagccaga tgcgctccga atacgaaaat acccgcgcca cgattcccca gattgagaag
     4741 agcatccacc agcaggaaaa tgccctgaac ctcctcttgg cccgcaatcc gggtccggtt
     4801 gtccggggcc tgaccatcga tcagttgaat tttccccatg tccccgaagg gctgccttcc
     4861 gaacttttgg agcggcgccc cgacatccgt caggccgagc aggacctgat tgccgccaac
     4921 gccagaatcg gcgtcgccag ggcggcctat ttccccgcca tttccctaac gggaacgttg
     4981 ggctcagcca gcgcggatct ttccaatctt tttacaggtc cggcttatat ctggaatgcc
     5041 ggcgcatccg ttaccgttcc catttttacc gccgggcgca cctggggaca ggtaaaggcg
     5101 tccgaggcgc tccagaaaca ggctctgctg acctaccaga aaacgatcca gacggccttc
     5161 cgggaagtgg aggattccct tgtggatcaa aaccggacaa gggccaagct tgaggctctg
     5221 ttcaatcagt tgaccgccct gcgaaactac cggaacctcg cgcttctgcg ctatgaaaac
     5281 ggctacagca gcaacctgga agtcctcgac gcggagcgca atcttttcag catcgagatt
     5341 acctacatcg aaacacgcgg cggtcttttt aacgctttca tcagccttta caaggcgatg
     5401 ggcggcgggt ggggcgtgga cggatctcct ccaccgaaag ccccggcggc ggagaaataa
     5461 actttttgac aggcacatgg aaaggtcatg gcaaaatcac gaaaccgcaa aacgctgacg
     5521 gctctgtctt tgcagggcgg cggcgcgctc ggagcgtatg aatacggcgt actcaaagcg
     5581 ctttacgaaa agcgcggcaa aggcttcacc ccaaaggttg tggccggcgt gtccatcggc
     5641 gccatcaatg ccgcgctgct ggtcggcgcg aaaggcgatc cgcttacagc cctcgaccgg
     5701 ctctggcggg aacggttatg cgtatcctgg ccttcccttc ccgattgctc ctgggccggc
     5761 cttaccccct ggtccgcaaa cagcctcgcc gaacggaacc tttccttctt tgccaattcc
     5821 ggcatgtatt gcctgaaacc ggaattcttc ctcatgcccg cgctcgcctg gctgaaaaac
     5881 agcagcctct acgacacatc gtttctgcgg gaaacactcg cggatttcgt cgatccggaa
     5941 aagctcaatc gcccggaaaa aacgcgcctg atcgtgacgg ccgtgaacgt caaaaccggc
     6001 cagttgtcgc gtttcgacaa cagccgaatg gagctgacct ttgaccacat catcgccagc
     6061 ggaagttttc ccgtcacttt tcccatgacg cagatcggtg aagacttttt ctgggatggg
     6121 ggcgtcttca tgaatatgcc ggtcggggaa gcggtgaacg ccctggaaca gatcgaagcg
     6181 gacaatcccg acgtcgagcg ggaagtgatc gtcatcgaac tccaccgcat gcaggccgac
     6241 cttccgaaaa ccataccgga ggccgccgaa cgcttctgca atctgatctt ttcgggaaaa
     6301 ttcgccctgg accggaaact gtatgaccgc tatggagctt tcgtggacct gatgggtgaa
     6361 atcgaccgga ccattcccgc cgacagcccg atccgaagcc acaaaggcta caaaatgatg
     6421 acccgccacc gaaagattga ccgcgccatg gtcatcggcg aagaaggcac gggcgcaacc
     6481 ggttccggcg gcgatttttc acgaaacgcc ctgaaccggc gcattgaagc cggttacggc
     6541 gacgccatgg cttttttcaa aaaaaaccgc taggtccggt ccacggcaga aaagcctcgc
     6601 ccgcccgctg cgcagcgttt gcggggctca gccggggagc tcccgaatct cttttgccag
     6661 ccgcgtcaga tcctcccgcc ccagttccag ggaaaccctg ccgccgcccg gatgggcgaa
     6721 gatcagcgcc gcgccgtttt ccgttttctc aacctgccat ttcgtccccg ccgggaaccg
     6781 gacctgctcg gcaagccagt cgtcggcttc gaattctgca atttgaatca gaacgccgaa
     6841 gccgtgccgg ggatgaatga aaatttcctt ccagacgcct cccggatatt cattgaaacc
     6901 gaaataggga attccgtggg cttccagatg cgccatcgcc tttcggatat ccggcgtctg
     6961 cagcgtgata tggtgaaccc ccgcctgtct ctggctcaga aagccgtcca gaaaactccc
     7021 gggctccgtg ggcgagatga tctccaggcg ggagagatcg cccagggaat aaatctgcca
     7081 gaaaaacctg gaaacagggt cggccatgcc ggcccccgca accgccccca gaatgtcgcg
     7141 gaaaaaatgc tcggccttct ccttttcctg atccctcacc gcgatggaaa catggtcaat
     7201 gcgtgaaatc attttttttc atctcctcga taaaattcca gccatgaaaa aatcgcgggc
     7261 cctttgcgtg cgggcccgcg atctcgtttc agtctgcggc ggaatctttc tttccggccg
     7321 gtttgttccg tccgctgtca gctcggcatg gtggtaaaga cgccccgcgg atccacctcg
     7381 gtgcggatga ccttgaggca atcgggcgta atcagtttgg tttcgggaat cgctccctcg
     7441 ggcttgagaa gctcaaaacc ggtgagctgc tgggcgagct cgaaattgac gccgggggtg
     7501 agcgatttga cccgcatgcg tttgctgacc ggatgaaaat catagacgcc ccaggggctg
     7561 atcaccgcgg cggggccgtt gccgatgagg cccgcctctt tgcgggagtc gccgccttta
     7621 agccagccga ccgaggttgc gaaatcaacc ttctcgggaa acttgtcggg cgtctgcagc
     7681 ccgaccagaa ccatcttctc ggtaagcgaa gacagatcat tgttgccgcc gctgccggtc
     7741 aggcggactt tgggatgatg gtaatccttg ccgatcatgt gggtgttgat gttgccgtac
     7801 atatccacct gggcgaatcc cagaaacccg atgtccacgt agccgttgta gcactgcgcg
     7861 aacgaatagg ac
//
LOCUS       DAOSIM010000083         7820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_8597_length_7820_cov_8.120026, whole genome shotgun sequence.
ACCESSION   DAOSIM010000083 DAOSIM010000000
VERSION     DAOSIM010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8597_length_7820_cov_8.120026"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>880
                     /locus_tag="PLR47_05005"
     CDS             <1..>880
                     /locus_tag="PLR47_05005"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOG12334.1"
                     /translation="IDRDGRWYVQDRPEIIADKDFGRILSLKKKGVWLVNHFEDQSRL
                     HLYDMESRGKKVVVDLVFPVLHGTYGEDGTMQGLLELARVPYVGVDVAGSAIGMDKDV
                     AKRLLAQAGVPVVPWMTVSRPEWLAGPQKILRGALRRLEMPVFVKPLCTGSSVGIKKV
                     KVKKDLASAVDYAFQFDTRIMIEKGIDCREIECAVLGNENPQASVLGEILPTHEFYSY
                     EAKYLDPEGAKMSIPAKIPARLAAAIRKCAVLGFQALNCNSMARVDFFLDKRTNEFYL
                     NEINTLPGFTGISMYPK"
     assembly_gap    881..890
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1055..1966
                     /locus_tag="PLR47_05010"
     CDS             1055..1966
                     /locus_tag="PLR47_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOG12335.1"
                     /translation="MSELSAAVSNSTKIRLLTLMMAMEIYTVGVSIIGILTSNSIVFI
                     ANFVIAFTGALASGLSLYTVRRMSRGTDLLNTYGLGRMETISSIVVGTTMLLAALFVG
                     YETVSKILHPSSQHGGLAAMLLTFASFAASTWLWIRNYRLSKKEHSPIFSSMWRMARM
                     GALEDLLIILAVGISLVFSDATWTRYLDVCATSILMISIVKSIYDVFSGSISDLLDRS
                     LDEYNMLIILRELIANFDAYDQIHGVRTRRSGSHTFIEIFLEFASDAKVEEVYRLKDR
                     LEAELKKQMPNAQIMLIPSRLTGNARF"
     gene            2287..3201
                     /locus_tag="PLR47_05015"
     CDS             2287..3201
                     /locus_tag="PLR47_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOG12336.1"
                     /translation="MMKNAQPLENRSRGEEALISDIKGPFLFPVPDIRRAENALSGED
                     NISQKIIDCFPGWFYLMDQQLNLVFWNDNFRQATGCSNEELRGMSVLEFYKEAQQAFI
                     AEELIRVFRLGEYAAEADIAFREGTVRTFYFNFKQVCYGNAPCLAAAAFDVSGWKGGH
                     VHFSRQPQNTSLSLQVSLRSEKEGRQATEDKLKANLEDLVIPHLKKLKATNLNATQRK
                     HLEALENRLNDVLSPFVNSLLLIYSNLTPQEIQIADLVRSGNGTKEIADTLNTSVNTI
                     ATHRNNIRKKLNLRNTKVNLRTHLQTIL"
     gene            3236..4060
                     /locus_tag="PLR47_05020"
     CDS             3236..4060
                     /locus_tag="PLR47_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12337.1"
                     /translation="MEALHQAIDFKPRYMEVTDSADAGRPVRKADPSHAVAAGTSHLF
                     NHHLVPALLNIEMAMNKLPKNGKTFRLLSDAMKSACQAAEICRMLMGSLGFCLGCGSS
                     GRAPVDVSGACLRFLPVFRALVLPNTRLEADLPPGGPSVRISRDLLNQVLINLIRRAG
                     EFFEGAPGVFYFRVRTAGPEELPMHAPFSHGSRSAYACVEIESRGCPSAERDGSAMER
                     TVSAAGSILSVYGGYAAAQTNEAGKVAFRIFLPVCKTEPPPSGTKVVSGSIAELFS"
     gene            complement(4112..4420)
                     /locus_tag="PLR47_05025"
     CDS             complement(4112..4420)
                     /locus_tag="PLR47_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12338.1"
                     /translation="MNISEWLDQKEAQGFDVSRAVLPEELEREEEPDETVYFKEIRPC
                     SVLCTGDHPFAAVERFGRWYRSRGKEKEKGPHTGKPQWWLFTRDKDLAIQTARSRIEK
                     "
     gene            complement(4445..4654)
                     /locus_tag="PLR47_05030"
     CDS             complement(4445..4654)
                     /locus_tag="PLR47_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12339.1"
                     /translation="MAENKKEGTLINCFFCRHFYLTGRVKSPYGCRAHGFTTFRMPSV
                     DVFEASDEDCALLDRREPDEHPHDG"
     gene            complement(4755..7733)
                     /locus_tag="PLR47_05035"
     CDS             complement(4755..7733)
                     /locus_tag="PLR47_05035"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOG12340.1"
                     /translation="MTTIDVLQPTLKAGDVVHGFIVRRVEVIETMRLAAYEMEHRKTG
                     AKVLHLHAADRENLYVIGFRTPPSDSTGLPHILEHSVLAGSEKYPLRDVFKELMRSTM
                     QTFINAFTYPDKTLYPAASQIKTDFYNLARVYTDLVLHPRLLRETFLQEGHHLEFAKP
                     GDPESDLIISGIVYNEMKGAYSSPESLMYKDIQQNLYPDTVYARDSGGDPNVIPTLTY
                     EQFRDFHRKYYSPTNARFFLYGDIPTAEHLAFLAQMLDGFERVEVDSSIASQPFFDGP
                     RRVTGAYPIDPDEPGENKTHVNVAWLLAENRDFETALLLSILSGLLVGSAASPLRRAL
                     VDSGLGEDLSPVSGIESDLKQLMFCAGLRNAGSADIPAIEKLILDTLKKIVDEGFDPE
                     LIEGILHQVEFHGKEISRGSYPYGITLMGGVFQTWLYDGDPLIGLDFSRVIENIRRFL
                     KDDPQIFQKVAQKWLLDNPHRLLAVMEPDPDFNQKNEARLRKKMADLKAGLSKEQLRE
                     IDEQAALLKAFQSADDSSAAQACLPKLKMEDLPRRVETIPTRRATAVPVPALAHDLFT
                     NGIAYADLAFDTASVPEDLQMLLPLLGKIMSGMGAAGLTYDEMAKRIALQMGGFGYDL
                     SCGRSADAGCVWQKMIFSFKALYRNVPNACRTVRDILCLGDLNDESRMRDLIAERKNN
                     LLASVVPSGHVFAKRTAAAGLTLPAHRDEQWHGRTQLRFVQQQAENFDASKNLLREKL
                     DRLRKILFTRKNLTVNLTADADGLRLLEEQIGVLLESLPAGRLDEKAAPPALSPVRSG
                     IAVPAQVSYVASAMKAPAYNDPDSALLMLASRELSNTYLYQRIRVQGGAYGGMTSFDA
                     MQGVFAFLSYRDPHIVETMDVFEAAKRFYSENEMNHDDLEKAVISALGALDKPLDPSG
                     RGYAALLRNFSGVTDDMRQFFRDRLFAATPGQVRQALSAYFEAASPSGSVAVYSSQEK
                     LEEANQLLEKKLKLEKLSEG"
BASE COUNT         1638 a         2159 c         2283 g         1730 t
ORIGIN      
        1 cattgaccgc gacggacgct ggtatgttca ggaccggccg gaaatcatcg ccgataaaga
       61 tttcggcaga atcctgtctt tgaaaaaaaa gggcgtctgg ctggtcaatc attttgaaga
      121 ccaaagcagg ctgcaccttt acgatatgga atcccgcgga aagaaggttg tggttgatct
      181 ggtttttccc gtcctgcacg gcacgtacgg agaggacgga acgatgcagg ggcttctgga
      241 actggcccgg gttccctacg tgggggtgga tgtcgccggc tccgccatcg gcatggacaa
      301 ggacgtcgcc aagcgtctgc tcgcgcaggc gggggttccc gtcgtgccct ggatgacggt
      361 cagccgaccg gaatggctgg ccgggccgca aaaaattctg cgcggggcgc tgcgccggct
      421 tgaaatgccc gtgtttgtca aaccgctgtg caccggctct tccgtcggca tcaaaaaggt
      481 gaaagtcaaa aaagatcttg cttccgccgt ggattacgcc tttcagttcg acacgcggat
      541 catgatcgag aaaggcatcg actgccgcga gattgaatgc gccgttttgg gcaatgagaa
      601 tccgcaggcg tcggttctgg gcgagattct gcccacgcat gaattttatt cctatgaggc
      661 caaatatctg gaccccgagg gcgcaaagat gagcattccg gcgaaaatcc cggcgcgcct
      721 cgcggctgcc attcgcaagt gcgccgtcct cggctttcag gcgctgaact gcaactccat
      781 ggcgcgagtg gacttctttc tggataaaag gacaaacgaa ttttacctca acgaaatcaa
      841 cacgctgccc ggatttaccg gcatcagcat gtatcccaag nnnnnnnnnn ttcctacagc
      901 gctctcttgg atcgtctggt ggagcttgcg ctggcgcgcc accgtgaaaa aacgtctgtt
      961 gtgaccggct gaaagtcgca gagacttcga cgatccggtc gtttgggctt gagttgaaat
     1021 ttcatgaatg agaaggagga agaattaagg agatatgagc gaactttcag ccgcagtttc
     1081 caattcgaca aaaatccgcc tgctgacgct gatgatggcc atggaaattt acaccgtcgg
     1141 cgtttccatc atcggcattc tgacgtccaa ttccattgtc tttatcgcca atttcgtaat
     1201 cgcttttacg ggcgctctgg cctccggcct ttcactttat accgtgaggc gcatgtcgcg
     1261 cgggaccgat ctgctcaata cctacggcct ggggcgcatg gaaaccatct ccagcattgt
     1321 ggtcggaacc accatgctgc tggccgctct gtttgtcggc tatgaaaccg tctccaaaat
     1381 cctccatccc tcctcgcagc acggcggtct ggcggccatg ctgctgacgt tcgcttcctt
     1441 tgcggcgtcc acctggcttt ggatacgcaa ctacaggctg agcaaaaaag agcactctcc
     1501 tattttcagc tccatgtggc gcatggcgag aatgggcgcg ctggaggatc tgctgattat
     1561 tctggcggtg ggcatttccc ttgttttttc cgacgctacc tggacgcgct acctggatgt
     1621 ctgcgccaca tcgattctga tgatcagcat cgtcaagtcg atttacgatg ttttctccgg
     1681 atcgatcagc gatttgctgg accgcagcct cgatgaatac aacatgctga tcattttgcg
     1741 cgaactgatc gccaacttcg acgcctatga ccagatccac ggcgtccgca cgcgccgttc
     1801 gggaagccat accttcatcg agatctttct ggagtttgcg agcgatgcga aggtggaaga
     1861 ggtttaccga ctgaaggacc ggctggaggc ggagctgaaa aagcagatgc ccaacgccca
     1921 gatcatgctg attccgagcc gcttgactgg aaacgcaagg ttttgatttt atttgataaa
     1981 caataataaa acgaatcaac cctttcaggg aaggcgccct ctgtttcaaa tgcggcggcg
     2041 ctcaggttct cccggcttcc ggcgtttttc agcccgggaa aagctcattt gccatgtatt
     2101 tcttatccat aataattcca tgaattttca ttgaacttcc cctttgacaa gtatttccat
     2161 aaacggttct aatcatcctg aaaataccgt ccggcagtcg agctttccgg cgcgggccat
     2221 cggtacggtc aggggttgcc ggaacgcatc caggaaaaag gaactttttc aggagagatc
     2281 agaaccatga tgaaaaacgc acaacctttg gaaaaccgaa gccgtgggga ggaagcgctc
     2341 atttccgaca tcaaaggacc gtttcttttt ccggttccgg atatccgccg ggcggaaaat
     2401 gcattatccg gagaagacaa catcagccaa aagatcatcg attgttttcc cggatggttt
     2461 tacctgatgg atcagcaatt aaacctggtc ttctggaacg acaacttccg gcaggcgacc
     2521 ggctgttcaa atgaagagct tcggggaatg tcggttctgg aattttataa agaggctcag
     2581 caggccttca tcgccgaaga attgatcaga gtttttcgtc tgggcgaata cgccgccgaa
     2641 gcggacatcg cattcaggga agggacggtc cggacgtttt atttcaattt caaacaggtc
     2701 tgctacggca acgccccctg cctcgcggcg gcggctttcg acgtttccgg gtggaaaggc
     2761 gggcatgtgc atttttccag gcagccgcaa aacaccagtt tgtccctgca ggtgagcctc
     2821 cgctccgaaa aagaagggcg gcaagccact gaggataaac tgaaggccaa cctggaggat
     2881 ctggtcattc cgcatttgaa gaaactgaag gcgacgaacc tgaacgccac gcaacgaaag
     2941 cacctggagg ccctggagaa ccgtctgaat gatgttttgt caccgtttgt gaacagtctg
     3001 ctgctcattt attcaaatct cacccctcag gaaatccaga ttgccgatct ggtccgcagc
     3061 ggcaacggca ccaaagaaat cgccgatacg ctcaacacgt cggtgaacac gattgcgacc
     3121 catcgcaaca acattcggaa aaaattgaac ctgcggaaca cgaaagtcaa ccttcggacc
     3181 cacctgcaga ccattttata aaagatcgca aacacgaaaa ggaacccgga agcttatgga
     3241 agcacttcat caggcgattg atttcaaacc aagatacatg gaggtcacag actccgcgga
     3301 tgccgggcgg ccggttcgga aagccgatcc ttcccatgcc gtcgcggcgg gcacgtccca
     3361 tctcttcaac catcatcttg ttccggcgct tctcaatatc gagatggcga tgaacaagct
     3421 gccgaagaac ggaaaaactt tccggctgct ttccgacgcc atgaaatccg cctgccaggc
     3481 ggcggagatc tgccgaatgc tgatgggcag tctgggcttt tgtctgggct gcgggtcatc
     3541 cggtcgggcg ccggtggacg tgtccggggc ctgtttgcgg tttctgcccg ttttccgggc
     3601 gctggttttg cccaacacgc ggcttgaagc cgatttgcca ccgggcggac cgtcggtccg
     3661 gatcagcagg gatttgctga accaggtttt gatcaacctg attcgaaggg cgggggaatt
     3721 ctttgaagga gcgcccggcg ttttttattt tcgcgtgaga acggccggac cggaagaact
     3781 tccgatgcat gccccgtttt cgcacggctc gcggtccgct tacgcgtgcg ttgaaattga
     3841 aagcagggga tgtccttcgg cggagcggga cgggagtgcc atggaaagaa ccgtgtccgc
     3901 ggccggttcc atcttgtccg tgtacggggg atatgcggcg gcgcaaacca atgaggcggg
     3961 caaagtcgcc ttcagaatct ttttgccggt ctgcaaaacc gaaccgccgc cttccgggac
     4021 aaaggtcgtt tcgggaagca tcgcggaatt gttctcgtaa acggggatcc gcagattccg
     4081 acgtcagtcc gggttcgccc gggggcggtc tttacttttc gatgcgggac ctggccgttt
     4141 gaatggcgag atctttatcc cgggtgaata gccaccactg cggtttcccg gtgtgaggtc
     4201 ctttctcctt ttcctttccc cggctgcggt accagcggcc gaatcgttcg accgcggcaa
     4261 agggatgatc gcccgtgcac aaaacgctgc aggggcggat ttccttaaag taaaccgttt
     4321 catccggttc ttcctcgcgc tccagctctt cgggcaggac cgcgcgtgac acgtcgaatc
     4381 cctgagcttc tttttgatcc agccactccg aaatgttcat attcttcttc ctttcccgaa
     4441 ggcgttatcc gtcgtgaggg tgttcatccg gttccctgcg gtccagaagc gcgcagtcct
     4501 cgtccgaagc ttcaaacaca tccacggagg gcatgcggaa ggttgtgaaa ccgtgcgccc
     4561 ggcagccgta gggagacttt acccggccgg tcaggtagaa atgccggcag aaaaagcagt
     4621 tgatcagggt tccttctttt ttgttttccg ccattgaaaa gttccgatga aatgctgaag
     4681 gccagaggct gtagacgata ggtgtgcatt atttgtgatc tctagcctat cgccaatggc
     4741 ccatagcctg ttttctatcc ttccgacaat ttttccagct tcagcttttt ttcgagaagc
     4801 tgattggcct cttccagttt ttcctgcgag gaataaaccg cgacggagcc ggagggcgaa
     4861 gcggcttcaa aataggcgga caatgcctgg cgcacctgcc cgggcgtcgc ggcaaacagc
     4921 cggtcccgga aaaactggcg catgtcgtcg gtcacgccgc tgaaattcct cagcagggcg
     4981 gcatacccgc gccccgaagg atcgaggggc ttgtccagcg cgccgagcgc gctgatcacg
     5041 gccttttcca gatcgtcatg attcatttca ttttcggaat aaaaccgctt ggcggcctca
     5101 aagacatcca tcgtttccac gatgtgcggg tctcgatagg acagaaacgc gaacacgccc
     5161 tgcatcgcgt cgaaagaagt catccccccg taggcgccgc cctgtacgcg gatgcgctgg
     5221 tacagatacg tgttggaaag ctcgcgcgag gccagcatta aaagggcgga gtccggatca
     5281 ttgtaggccg gcgccttcat cgccgaggcc acgtaggaaa cctgcgcggg gacggcgatg
     5341 ccggagcgga cgggcgaaag cgcgggcggc gcggcttttt catcaaggcg tcctgcgggc
     5401 agactttcca gcaatacgcc gatttgctct tccagcagcc tgaggccgtc cgcgtcggcg
     5461 gtcaggttga cggtcagatt cttccgggtg aagagaattt tcctgagccg gtccagtttt
     5521 tcccgcagga ggttcttgga ggcgtcgaaa ttttccgcct gctgctggac aaagcgaagc
     5581 tgcgtccggc cgtgccactg ttcatcgcgg tgcgcgggca gcgtcagccc cgccgccgcc
     5641 gtccgtttgg cgaagacatg tccggaggga acgacggagg cgagcaggtt gtttttgcgc
     5701 tcggcgatca agtcgcgcat tctcgactca tcgttcaggt ctccaaggca gagaatgtcg
     5761 cggacggtcc ggcaggcatt tggaacattg cggtaaagcg ctttgaagga aaaaatcatt
     5821 ttctgccaga cgcagcccgc gtcggcggac cggccgcagg ataaatcgta gccgaaaccg
     5881 cccatttgca gggcgatgcg cttggccatc tcatcgtacg tcagcccagc cgcgcccatg
     5941 ccgctcatga ttttgcccag aagcggcaga agcatctgca ggtcctcggg aaccgaggcg
     6001 gtgtcaaagg ccaggtcggc gtaagcgatg ccgttggtga acaggtcatg cgcgagcgcc
     6061 ggaaccggaa ccgcggttgc gcgccgggtc ggaatcgttt ccacccgccg gggaaggtct
     6121 tccattttca gcttgggcag acacgcctgc gccgcggaag aatcatccgc cgactgaaag
     6181 gccttgagaa gcgccgcctg ctcgtcgatt tccctgagtt gctctttgga caggccggcc
     6241 ttgagatcag ccattttctt tcgcagccgc gcttcattct tttggttgaa atcgggatcc
     6301 ggctccatca ccgccagcag gcggtgagga ttgtccagca gccacttttg agccactttc
     6361 tggaagattt gcggatcgtc cttcaggaag cggcggatgt tttcaatcac gcgcgaaaaa
     6421 tcgaggccga tcagggggtc gccgtcgtag agccaggtct gaaaaacgcc gcccatcagc
     6481 gtgatcccgt aagggtagga gccgcgggaa atttccttgc cgtgaaactc gacctggtgc
     6541 aggatgcctt cgatgagctc cggatcgaac ccttcgtcga cgattttttt cagggtgtcg
     6601 aggatcagct tctcgatggc gggaatgtct gcgcttccgg cgttgcgcag acccgcgcag
     6661 aacatcagtt gcttgagatc ggattcgatg ccggacaccg gcgagaggtc ttcccccagc
     6721 cccgaatcga cgagcgcccg gcgcagcggc gaggcggcgc tgcccacgag caggccggag
     6781 aggatggaga gcaggagcgc cgtttcgaag tccctgtttt ccgcaagcag ccaggctacg
     6841 ttgacgtggg ttttgttttc ccccggctcg tccggatcga tgggataggc gccggtcacc
     6901 cgccgggggc cgtcaaagaa aggctggctc gcgatggacg aatccacctc cacccgttca
     6961 aacccgtcga gcatctgagc cagaaacgcc aaatgctccg cggtgggaat gtcgccgtag
     7021 aggaagaagc gcgcgttggt cggggagtag tatttgcggt ggaaatcccg aaactgttca
     7081 tacgtgagcg tgggaatcac gttcgggtct cccccggaat cccgcgcata cacggtgtcc
     7141 gggtaaagat tctgctgaat gtccttgtac atcagcgatt cgggcgacga gtaggcgccc
     7201 ttcatctcat tgtaaacgat gccggagatg ataaggtcgc tttcgggatc gccgggcttt
     7261 gcgaattcca ggtgatgccc ttcctgcaga aaggtttcgc gaagcaggcg ggggtgcagg
     7321 accaggtccg tgtaaacgcg cgccagattg tagaaatccg tcttgatctg gctggccgcc
     7381 gggtaaaggg ttttgtccgg gtaggtgaag gcgttgatga aggtctgcat ggtggagcgc
     7441 atcagctcct tgaagacgtc ccggagcgga tatttctccg agccggccaa aacggaatgc
     7501 tccaggatgt ggggcaggcc cgtggaatcc gaaggcggcg tccgaaagcc gatgacgtaa
     7561 agattttccc ggtcggcggc gtgcaggtgc agcactttgg cgcccgtttt ccggtgctcc
     7621 atctcatagg cggcaagccg catcgtttcg atgacttcga cgcgccttac gataaagccg
     7681 tggacgacat cgcctgcctt cagcgttggc tgcaatacat cgatggttgt catttccgga
     7741 cgctccttgc cgttggggga ttatggccgc ggacaatcct gaatcgtcac gcggcggttt
     7801 tgcgcctggc cttccggcgt
//
LOCUS       DAOSIM010000084         7798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_8627_length_7798_cov_7.758362, whole genome shotgun sequence.
ACCESSION   DAOSIM010000084 DAOSIM010000000
VERSION     DAOSIM010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7798
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8627_length_7798_cov_7.758362"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLR47_05040"
     CDS             <1..587
                     /locus_tag="PLR47_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOG12341.1"
                     /translation="PNIAYLLLLIGLAGLYFEFSTPGAILPGVIGGISLLLAFFGLST
                     LPVSYTGILLILFSVILFIAEIKIMSHGMLTVGGVISLALGSFLLFDSPEPALRISLQ
                     VLLPAVLVASGFFIVIILLAVKAQMRKHYSGTEAMIGGEGEAATDVHGAGKVFFQGEY
                     WTARSQRAIPKGASVRIVKVEGLKLVVDEIKNNE"
     gene            614..>928
                     /locus_tag="PLR47_05045"
     CDS             614..>928
                     /locus_tag="PLR47_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOG12342.1"
                     /translation="MLGSLPLPLLVVIVLVVMFLASAIKILNEYERAVLFRLGRLRDI
                     KGPGLIVIIPGIDKIVRIDMRTITMDVPPQDVITKDNVSIKVNAVVYFRVLDANAAVV
                     NVE"
     assembly_gap    929..984
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <985..1294
                     /locus_tag="PLR47_05050"
     CDS             <985..1294
                     /locus_tag="PLR47_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOG12343.1"
                     /translation="WGIKVSLVEVKHIDLPEEMKRAMAKQAEAERERRAKVINAEGEF
                     QAAQKLIDAAALMETQPMSLQLRYLQTLNQIAAENNSTTVFPIPIDLFKPFLKLAEKS
                     "
     gene            1356..1667
                     /locus_tag="PLR47_05055"
     CDS             1356..1667
                     /locus_tag="PLR47_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12344.1"
                     /translation="MPNEKRNVLTGGLILITLGVLIFISKTTSCSFGQTWPILLIVIG
                     ISTLIYSIRAIEGWFIATVGVIFLVIELYGFDLYRYSQYILPVILIVLGVYVLLKRRK
                     R"
     gene            1696..2403
                     /locus_tag="PLR47_05060"
     CDS             1696..2403
                     /locus_tag="PLR47_05060"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtnX-like HAD-IB family phosphatase"
                     /protein_id="HOG12345.1"
                     /translation="MRFQQKKALVLSDFDGTISRVDVGNSVLDRFTPEGWEAIDREVV
                     KGSLGSREAYEKIAPMVRVDVNRLRTHVLKHAAIDPFFVRFCRLAGKRGVDVKIVSDG
                     LDFYIRAILEKHRLGEIEFYSNRVVFGREEAVAFEFPQANALCGRCGTCKNKILHDHR
                     LMYEKIIYVGDGHSDICPSQCADLIFAKGELLRKCERESAVPYRPFHDFREVSEYLKK
                     TFDKRRAIRGATREKES"
     gene            2400..3584
                     /locus_tag="PLR47_05065"
     CDS             2400..3584
                     /locus_tag="PLR47_05065"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOG12346.1"
                     /translation="MSISKKIKASMSQSSLIRKMFEEGNLLKSRHGHDKVYDFSLGNP
                     NVDHPREFKKELIRVARQEIILKHGYTPNAGYPETRRAVAQTISRSIGLKMDADHIVM
                     SCGAGGALNVILKSILDPGDEVLILKPFFVEYPFYIENYGGVVKIADTRPDFSPDLEA
                     IARMITRRTKALILNSPNNPTGRVYSKTNIAGLARLLREKEKKYSKAIYLLSDEAYAQ
                     ILYDGIQLPSVLTAYADSIVAYSYSKTLSLPGERIGYIAVNPKIHEADDLIRALILCT
                     RIMGFVNAPALMQRIVANLQHITVDVKIYQKKRDLLCGGLARAGYQFIKPQGAFYLFV
                     KSPVPDDVAFAQRLVQKNILVVPGSGFGTPGYFRIAFCVDDATIRNSMKGFAAAIRDC
                     RG"
     gene            complement(3764..7726)
                     /gene="hrpA"
                     /locus_tag="PLR47_05070"
     CDS             complement(3764..7726)
                     /gene="hrpA"
                     /locus_tag="PLR47_05070"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HOG12347.1"
                     /translation="MLSSNHPHNGLIRRVERHAREIMIADYHRLLQTLKTANASSVGQ
                     PDRTALAASRLQSRMDASRRRVQRRGQSVPSLNFPPELPICTKRQTIVDAIRKNQVVI
                     VTGETGSGKTTQIPKMCLEAGLGLRGVIGLTQPRRIAAVSIAARIASELGQDCGQAVA
                     YKIRFEEKTSAEPLIRVMTDGMLLTETVGDRNLLAYDTIIVDEAHERSLNIDFLLGYL
                     RSLLPRRKDLKLIITSATIDTAKFAEVFDGAPVIEVSGRMYPVEVRWRPLDPAKEEKG
                     ETTYVDEVVACVEDLQREGRFEDILIFLPTEQDIREACERIGKRCSGLVLPLFARLSS
                     EQQKLVFHSADRQKIIVATNIAETSLTIPGIKYVIDSGLARVLEYNPRSQTTGLPVKP
                     VSQSSAEQRKGRCGRVQNGICYRLYSAEDLASRPVYSTPEILRSNLAGVVLRMLSLNL
                     GMIDDFPFIDRPQPKSLRDAVDTLRDLGAIRRDQNEGEAGQYRLTPIGRTMAQFPLDP
                     RVSRMIIEAQKNGCVREIAVIAAALSIQDPRERPYDKIDQAAAAHAQFAHPESDFLAY
                     LQIWNRYSDYFEQMQPQGQRRKFCRDHFLSYRRMEEWRDVYSQILDILGGKAGAISRR
                     KPVRAEIDQEVSDKIHRSILSGYLSNIAQKKEKNFYTAAKSREVMLYPGSGLFNRGGP
                     WIVAAEISLTSRVFARNAANIQSEWLEELGGEHCRCQHWAAHWEKSRGQVVALEKVVL
                     FGLTIVEQRSVAYERINPEEAWRIFIREALVTGETGRKLPFLEHNLSLREKVKTMEDK
                     LRKRGIAADEETIAGLYEQRLPLLSDIRSLQKLIKDRGSDAFLRFREEDLIAAGPDLD
                     ELNRYPDQIRMGNAVLPCRYKFEPGRAGDGVTVRMPLGLIAGASPVNIEKHLPSLREE
                     KALHWLKSLPKNIRRNLPPLIEVAGRFNRQTAGEDQALPLALSRFLFDEYHLTVPAGF
                     WAPEKLPPHLQIRYSVIDDRGSEIRSSRDIGELQRELTEEIRRTSLEDCRRQWEKENL
                     TSWDFGDLPENVEIAGKHGVTGLAYPALQDQGGAVHLRIFADPREAAAVHAAGVAALY
                     AIHFEDRIKQLKKNISLGGELKEQASLLGSPRQVERSVLERVKRDLFRRSWRSPKEFM
                     KHADEVRPKILSYGQEVVAAIAPVLRSFAETHDGLQKLIFKNRPHPPLAEFLQATLAE
                     AHVLVPEDFILHCDFDRLRHLPRYLKALFLRAERGSLNLASTESKLRQAATYAVKLQE
                     IRGAMDTDASEEKRNKTDELRWMIEEYKVSLFAQELKTPYPVSPKRLNQLIQEIGNML
                     "
BASE COUNT         1592 a         2153 c         2229 g         1768 t
ORIGIN      
        1 atccgaatat cgcgtatctg cttttgctga tcggcctggc ggggctgtac tttgagtttt
       61 ccacaccggg cgcgatcctg cccggcgtca tcggcggcat ttccctgctc ttggcttttt
      121 tcggcttgtc cacgctgccg gtgagttaca cggggattct gctgatcctg ttcagcgtca
      181 ttttatttat tgcggagatc aaaatcatga gccacggcat gctgacggtg ggaggagtta
      241 tttcacttgc gctgggatct tttttgcttt ttgattcccc ggagcctgcg ctgagaattt
      301 ccctgcaggt tctgcttccg gccgtgctgg tggcgtccgg gttcttcatc gtcattatcc
      361 tgctggccgt caaggcgcag atgaggaagc actattccgg gacggaggcc atgatcggag
      421 gcgaggggga agccgcgacg gacgtccacg gcgccggcaa ggtatttttc cagggcgaat
      481 actggacggc ccgcagccaa cgggcgattc caaagggcgc ttcggtcaga attgtcaaag
      541 tggaaggcct gaaactggtg gtggatgaaa tcaaaaacaa cgaataaata tcgataacga
      601 aggaggaagg attatgttgg gttctttgcc gctgcccctg ttggtggtga tcgtgctggt
      661 ggtcatgttt ttggcttccg cgatcaaaat cctgaatgaa tatgaacggg cggtcctttt
      721 ccggctgggc aggcttcgag acatcaaggg accggggttg atcgttatca ttcccggcat
      781 tgataaaatt gtccggatcg acatgcgcac cattaccatg gacgtcccgc cgcaggatgt
      841 cattaccaag gacaacgttt ccatcaaggt caatgcggtg gtttatttcc gggtgctgga
      901 cgccaacgcc gccgtcgtga atgtggagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnctgggg aatcaaggtg tcgctcgtgg aagtgaagca
     1021 tattgatctg cccgaagaaa tgaagcgcgc gatggccaag caggccgaag ccgaacgcga
     1081 aagacgggcg aaggtcatca atgcggaagg agaattccag gccgcgcaga agctgattga
     1141 tgcggccgcc ctgatggaga cgcagcccat gtcgctgcag cttcgttatt tgcagacgct
     1201 gaatcagatt gccgcggaaa ataattcaac gacggtgttt ccgattccga tcgatttgtt
     1261 caagcctttc ctgaagctgg cggaaaaatc ataagcgcgc gccggggggg cgattccccg
     1321 gtgctcaaaa aacacattta ttgaaggaga aggatatgcc aaatgaaaag cgcaacgttc
     1381 ttaccggcgg acttattctg attaccctgg gggttttgat ctttatcagc aaaaccactt
     1441 cctgcagttt cggccagacc tggccgatcc tgctcatcgt catcggcatc agcacgctga
     1501 tttacagcat ccgggccatc gagggctggt ttatcgccac ggtcggcgtt atttttctcg
     1561 tcatcgaatt gtacgggttt gatttataca ggtactccca gtacattctg ccggtgattc
     1621 tgattgtact gggcgtttat gtgctgctga agcgccgcaa acgataggaa ccctgttgcg
     1681 aaaaaagcac ttgccatgcg ctttcagcaa aaaaaggctc tggttctcag tgattttgac
     1741 ggtacgattt ccagggtgga tgttggaaac agcgttctgg accggtttac accggaaggc
     1801 tgggaagcca ttgaccgcga ggttgtcaag ggcagcctgg gatcgcggga ggcctacgaa
     1861 aaaattgcgc cgatggtgcg ggtcgatgtc aaccgtctcc ggacccatgt gctgaagcac
     1921 gccgccatcg atcctttttt tgtccggttt tgccgtctgg ccggaaaaag aggagtggac
     1981 gtcaaaatcg tttccgacgg gctcgatttt tacattcggg cgattctgga aaaacaccgg
     2041 ctgggggaga tcgaatttta ttccaaccgg gttgtgttcg gccgggagga ggccgtggcg
     2101 tttgagtttc cgcaggccaa tgcgctgtgc ggccgctgcg gaacctgcaa gaataaaatt
     2161 cttcacgatc accggctgat gtacgaaaaa atcatctatg tcggcgacgg ccactccgac
     2221 atctgcccgt ctcagtgcgc ggacctgatc tttgccaagg gcgagcttct gcgaaagtgc
     2281 gagcgggaaa gcgcggttcc ctaccggccg ttccatgatt ttcgcgaggt ttcggagtat
     2341 ctgaagaaaa cgtttgacaa acggcgcgct attcggggag ctacccggga gaaggaatca
     2401 tgagcatatc caagaaaatc aaagcctcca tgtcgcagtc ttccctgatc cgcaaaatgt
     2461 ttgaagaagg gaaccttctg aaaagcaggc acggacacga caaggtctat gatttcagtc
     2521 tgggcaatcc caacgtggac catccgcgcg aattcaagaa ggagctgatt cgcgtcgccc
     2581 gccaggaaat catcctcaag cacggctaca cacccaacgc cggctacccg gaaacccggc
     2641 gggcagtcgc ccaaacgatc agccggtcca tcggcctgaa aatggatgcc gatcacattg
     2701 tcatgtcctg cggggccggc ggcgcgctca atgtgattct gaagtcgatt ctggatcccg
     2761 gcgacgaagt cctgatcctc aagccgtttt tcgtcgagta tcccttttat atcgaaaact
     2821 acggcggggt ggtaaagatc gcggacacac gccccgattt ttctccggat ctggaagcga
     2881 ttgcccggat gatcacccga cggaccaagg cgctcattct caactcgccc aacaatccga
     2941 ccggccgggt gtacagcaaa acgaatattg ccggcctggc ccggctgctg agagaaaagg
     3001 agaaaaaata cagcaaggcc atctatctcc tttccgacga ggcatacgcg caaattcttt
     3061 acgatggcat tcaactgcct tccgttctga cggcttatgc cgacagcatc gtcgcgtatt
     3121 cctactccaa aaccctgtcg ctgccgggcg aaagaatcgg ctacatcgca gtaaatccga
     3181 aaattcacga ggcggacgac ctgatccggg cgctgatcct gtgcacgcgg atcatgggat
     3241 ttgtgaacgc cccggccctg atgcagcgca tcgtggccaa tcttcagcac atcaccgtgg
     3301 atgtaaagat ttaccagaag aaaagggatt tgctctgcgg cggcctggcc cgcgcgggat
     3361 accaattcat caaaccgcag ggtgcttttt atctgtttgt gaaaagtccg gttcccgacg
     3421 atgtggcgtt cgcgcagagg ctcgtgcaga aaaatattct ggtggttccc ggaagcggat
     3481 tcggaacgcc gggctatttc cgcatcgctt tttgcgtcga tgacgcgacc atccggaatt
     3541 ccatgaaagg cttcgcagcg gccattcggg attgccgggg ctgaaccctt gcatttggca
     3601 ctcatggcgc cgaccaacgt agggcgggtc gcttgtgacc ttcaggttat catacccgcc
     3661 gaaccgacat gcaaggtcca tttggcgcac ctaaaggtgc gccctacata aaaaactgtc
     3721 aaaagacgga agctgttcct cgtgtttttg tcgagcgctc tgattacagc atatttccga
     3781 tttcctgaat cagttgattc aggcgtttgg gtgaaacagg gtagggggtt ttcagctcct
     3841 gggcaaaaag agacaccttg tattcttcga tcatccagcg cagttcatcc gtcttgtttc
     3901 gcttttcctc cgatgcgtcg gtgtccattg cgcctcggat ttcctgaagt ttaacggcgt
     3961 aagtcgccgc ctggcgcaat ttcgattccg tggacgccag gttcaggctt ccccgttcgg
     4021 cccgcagaaa gagagctttc aggtagcggg gcagatggcg cagacggtca aaatcgcagt
     4081 gcagaatgaa atcttcgggg accaggacgt gtgcttccgc cagggtcgcc tgcagaaact
     4141 cggccaaggg cggatgcggc cggtttttga atatcagttt ttgcaggccg tcgtgagttt
     4201 cggcaaagct tcgaagaacc ggggcgatgg ccgcgacgac ttcctgaccg taagaaagga
     4261 tcttcggccg gacctcatcg gcatgcttca taaactcttt cgggctgcgc caggagcgcc
     4321 ggaaaagatc tcttttcacc cgctccagaa cggatcgttc gacctgccgg gggctgccca
     4381 gcagggacgc ctgctctttg agttcaccgc caagggagat gttttttttc agttgcttga
     4441 tcctgtcctc gaaatggatg gcatagagag cggctacacc ggcagcgtgg acggccgcgg
     4501 cttcccgggg gtcggcaaaa atccgcagat gaacggctcc tccctgatcc tgaagcgccg
     4561 gataggctaa gccggtcacg ccatgctttc cggcaatctc aacattctcg ggcaggtctc
     4621 cgaaatccca gcttgtaagg ttctcctttt cccattgcct ccggcaatcc tccaaagagg
     4681 tgcgccggat ctcttcggtc agctcccgtt gcaattctcc aatgtcgcgg ctgcttcgga
     4741 tttcacttcc ccggtcgtcg atgacgctgt agcggatctg caaatgcggc ggcagttttt
     4801 ccggcgccca gaagccggcc ggcaccgtca aatgatattc atcgaataaa aacctgctta
     4861 acgccaaagg cagggcctgg tcttcaccgg cggtctgccg gttgaaccgt ccggcaacct
     4921 caatcagggg aggaagattc cggcggatat ttttgggcag ggattttagc caatgcaggg
     4981 ccttctcttc ccgcagggac ggcaaatgct tttcgatgtt taccggggag gctccggcga
     5041 tgaggcccag cggcatgcgg acggtcacgc cgtcgcccgc gcgccccggc tcaaacttgt
     5101 agcggcaggg caagacggca ttgcccatcc ggatctgatc cggataccgg ttcaattcat
     5161 cgaggtcggg tccggcggca atcaggtctt cctcccgaaa gcgcagaaaa gcatcgctcc
     5221 ctctgtcttt gatgagtttc tgcagggaac ggatatcgga aagaagcggc agacgctgtt
     5281 cataaaggcc ggcgatggtt tcttcatcgg cggcgatgcc gcgtttgcgc agcttgtctt
     5341 ccatcgtttt gactttttcc cgcaacgaca ggttgtgctc taaaaaggga agtttgcgcc
     5401 cggtctctcc cgtgaccagc gcttcacgaa tgaagattct ccatgcttcc tccgggttga
     5461 tccgttcata ggcgacgctt ctttgctcca cgatggtcaa tccgaataaa acaacttttt
     5521 ccagggcaac cacctgcccg cggctttttt cccagtgcgc cgcccagtgc tggcagcggc
     5581 agtgctcccc gcccagctct tcgagccact cgctttgaat gttggccgcg ttgcgggcaa
     5641 aaaccctgga ggtcaggctg atttccgcgg cgacgatcca gggcccgccc cggttgaaaa
     5701 gacccgaacc cggatacagc atcacctcgc ggcttttggc ggccgtgtaa aaattttttt
     5761 ctttcttttg cgcgatgttg gaaagatagc cgctcaggat cgagcggtgg attttgtcgg
     5821 aaacctcctg gtcgatttcg gctctgacgg gttttcgccg cgatatcgcg cccgcttttc
     5881 cgccgagaat gtccagaatc tgcgagtaaa cgtctctcca ttcctccatc cgccggtaag
     5941 atagaaaatg atcgcggcag aatttgcgcc gctgtccctg cggctgcatt tgctcaaaat
     6001 agtccgaata ccggttccag atctgcaggt aggcgaggaa gtccgattcc ggatgcgcaa
     6061 attgcgcgtg cgcggccgcc gcctgatcta tcttgtcgta gggcctttcg cgggggtcct
     6121 ggatgctcag agccgcggcg atcacggcga tttcacggac gcagccgttc ttttgagctt
     6181 cgatgatcat ccgcgatacc cggggatcaa gcgggaactg cgccatggtc cggccgatcg
     6241 gagtcagccg gtattgcccg gcttcccctt cgttttggtc gcggcggatc gcccccaggt
     6301 cgcgcagggt atcgacggcg tcgcgtaggc ttttgggctg cggccggtcg ataaagggaa
     6361 aatcatcgat catccccaga ttcagagaca gcatgcgcag caccacgccg gccagattgg
     6421 atcgcaggat ttccggcgtg gaataaaccg gccgcgaggc cagatcctcc gcggaataca
     6481 ggcggtagca gattccgttc tgcacgcggc cgcagcggcc ttttctctgc tcggcgctgc
     6541 tttgggaaac gggtttgacc ggcagcccgg tggtttggct gcggggattg tattccagca
     6601 cgcgggccag acccgaatca atgacatact tgatgccggg aatcgtcaga gacgtctctg
     6661 cgatattggt cgcgacaatg attttttgac ggtcggcgga atggaaaacc agcttctgct
     6721 gttccgagga gagcctcgca aacaggggca aaacaaggcc gctgcagcgc tttccaattc
     6781 gttcgcaggc ttcgcggatg tcctgctccg tgggcagaaa aatcagaatg tcctcaaagc
     6841 gtccttcccg ctgcaaatct tccacgcagg ccacaacttc atcgacatag gtcgtttcgc
     6901 ccttttcttc tttggccggg tccagcggcc gccagcggac ctccaccgga tacatccgtc
     6961 ccgagacttc gatgaccggc gccccgtcaa acacctcggc aaatttggcg gtgtcgatcg
     7021 tcgcggacgt gatgatcagc ttcaggtctt ttcttcgggg cagcaggctt cgcaaatatc
     7081 ccaaaaggaa gtcgatattg aggctgcgct cgtgcgcttc atcgacgatg atcgtatcat
     7141 acgccagcag gtttcgatcc ccgaccgttt cggtcagcaa catgccgtcg gtcatgaccc
     7201 gaatgagcgg ctccgccgat gttttttctt caaagcggat tttatacgcc acggcctggc
     7261 cgcagtcctg gccgagctcc gacgcaatgc gcgccgcgat gctgacggcg gcaatccttc
     7321 ggggctgcgt cagcccgata acgccccgca ggcccagccc cgcctccaga cacatcttgg
     7381 gaatctgcgt cgtcttgccc gatccggttt cgccggttac gatgacgacc tgattttttc
     7441 gaatggcatc gacaatggtc tgacgtttgg tgcagatggg cagttccggg gggaaattca
     7501 acgagggaac ggactgaccg cgcctttgca cacggcggcg cgatgcgtcc atccggcttt
     7561 gcagacggga cgctgcaagg gcggtccggt ccggttgccc gaccgaagag gcattcgcgg
     7621 ttttcagcgt ttgcagcagg cgatggtaat cggcgatcat gatctcgcgc gcgtggcgtt
     7681 ccacccggcg gatcagcccg ttatggggat ggtttgacga caacattttt ccccgtcatc
     7741 accggccgtc agcgccgcca gccgaaatcg agagccatgt cctggggcgc ttccagtt
//
LOCUS       DAOSIM010000085         7770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_8653_length_7770_cov_7.205703, whole genome shotgun sequence.
ACCESSION   DAOSIM010000085 DAOSIM010000000
VERSION     DAOSIM010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7770
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8653_length_7770_cov_7.205703"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="PLR47_05075"
     CDS             <1..1140
                     /locus_tag="PLR47_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOG12348.1"
                     /translation="LKEWFYYFYQCTECRRCSVFCPYGIDQAEITMIGRELLNLIGCS
                     INWVLEPAANCFRTGNHLGIQPGGIKDMLEFLADDIEDRTGVRVDVPINQKGADILFV
                     TPSGDYFADPGTYTCMGYMMLFHEIGLNYTFSTYASEGGNFGLFTSHEMVKRLNAKIY
                     AEAKRLGVKWILGGECGHMWRVIHQYMDTMNGPADFLEEPVSPITGTRFENARSTKMV
                     HIVEFTADLIKNGKLKLDPSRNDHIRLTFHDSCNPARAMGLFEEPRYIIQKSCNHFYE
                     MAEDTIREKTFCCGGGAGLGNDENMEMRMRGGMPRAMAVRDVVRKHGVNRLGCICAID
                     RVTLPPLMDYWVPGVTVMGVHELLANALVMKGEKERTTDLRGDSI"
     assembly_gap    1226..1325
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1474..1875
                     /gene="dsrJ"
                     /locus_tag="PLR47_05080"
     CDS             1474..1875
                     /gene="dsrJ"
                     /locus_tag="PLR47_05080"
                     /inference="COORDINATES: protein motif:HMM:NF038038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrJ"
                     /protein_id="HOG12349.1"
                     /translation="MKQHKWGLILLGLAIFAIAVTFPFWYGKGKTAPPVLSLETPEIA
                     GLADKKCVEDAVFMRANHMKMLQAWRDEAVREGKRVYTANDGRTFEKSLTGSCLQCHS
                     NKEHFCDRCHHYVGAAPSCFDCHIVPAEVKK"
     gene            1872..2654
                     /locus_tag="PLR47_05085"
     CDS             1872..2654
                     /locus_tag="PLR47_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG12350.1"
                     /translation="MTSSRRDFLKIAGASALTMMTPAIFVPAWAKDVIVIPPGQGLAL
                     KKALAAGRWAMTVDLTKCTADCTDCIAACHAIHNVPDFGNPKDEIKWIWKESFASVFP
                     GEQNAYSGEKLQKLRPPVFCNNCENPPCVRVCPTGATFQRPDGIVMMDYHRCIGCRLC
                     MAACPYGARSMNYRDPRPCIAKINPDFPTRSKGVVEKCNFCEERLAKGTLPACVEACR
                     QKALTFGDAENPRSEIRRLLKTRFSLQRRVHLGTGPQIYYLL"
     gene            2662..3855
                     /gene="nrfD"
                     /locus_tag="PLR47_05090"
     CDS             2662..3855
                     /gene="nrfD"
                     /locus_tag="PLR47_05090"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOG12351.1"
                     /translation="MLEKALHGSLRYRIWIACLILVGCAGFFFYLRQLDYGLGITGMS
                     RDVSWGLYIAQFTFLVGVAASAVMLVLPYYLHDYKAFGKITVLGELLAVAAVVMCILF
                     VFVDLGRPARVMNVLLHPQPSSILFWDMIVLSGYLLINLVTGWVILACTKKEIPPPGW
                     IKPVIYLSIPWAISIHTVTAFIYAGLSGRSFWLTAIMAPRFLASAFASGPALLILVCL
                     ILRRWGRFDAGEKAIQALAKIVAYALAVSIFFVGVEMFTVFYSQIPEHMHHFQYLFMG
                     LEGHHNLVAWMWIFAVLSIAAFVMLILPGVRRNEVWLALACIAVFVSLWIEKGLGLVI
                     TGFIPSPLGAITEYHPTGPEITITLGVWAAGFFLLTVFYKIFVHVRGGTDGSPCGPAV
                     DKMIH"
     gene            3907..5670
                     /gene="xsc"
                     /locus_tag="PLR47_05095"
     CDS             3907..5670
                     /gene="xsc"
                     /locus_tag="PLR47_05095"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: protein motif:HMM:NF005713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HOG12352.1"
                     /translation="MKKVKMTPSEAIVETLLAEGVDHVCGIVGSAFMDMLDLFPTAGI
                     KFIPVRHEQSAAHMEDAYCRISGRCGVVTGQNGPGITNMVTSVAAANMGHTPLVIISP
                     SAGTPTIGWDGFQECDQMSVFKSVTKAVVRVPHPKRAADCLRTAFRIAYADRGAVLYD
                     IPRDYFYGELEEKILKPSQYRVDARGCGSMEQLNRAAELLSKAKNPVIVSGRGVVDAD
                     ALDIVKAIAEHLTAPVAVTYLHNDAFYGNHPLSVGPIGYMGSKAAMKTLAKADVMLAI
                     GTRLSVFGTLPQYDINYFPENAKIIQVDINPKHIARTHPIEVGIIGDAKEASAEILKR
                     LKAMDGRRKPDEKRLAAVARERKKWEKEIVDLAMVDGNPMNPRRVLLEMSRALPDDAI
                     VTTDIGNVSSTANSYLKFNHPRRHIAALTFGNTGFAYPAALGAQIARPKAPVIAIIGD
                     GAWGMSLHEVSTAVEHNLPVIACVFRNMWWGAEAKNQVDFYNNRFIGVDIPNPESFVP
                     VAKAMGAHAMQVSRSQDIREAFQTCLKSRKPTVLEFLVDGKQLAPPFRKDALALPTRF
                     LSKYAHLDHRRWVDEGGSDDL"
     gene            5660..6499
                     /locus_tag="PLR47_05100"
     CDS             5660..6499
                     /locus_tag="PLR47_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG12353.1"
                     /translation="MIYNDLKGKTALVTGAGKKTGIGYAIAEKLASCGCNVIIADLGA
                     PPAPESPLQPVVSEDMKAIASELAKAYAVRTLAVAVDVADNQAIGAMVETIKKSFDHI
                     DILCNNAGASFGVPNTVLGYDEAAWMKTIDVNLFSVFRMSRAVLPLMAGKPASIINTA
                     SRAGKVPPIFNSAYAVAKAGVIMLTKVMAKELGGGGIRVNAICPGQIATDLEKWRFGL
                     EASFFGTTIEEREKEMCKTIPLGYIGLPRDAGDLVAFLASDASRYITGQAVNLDGGQC
                     MEL"
     gene            6562..>7770
                     /locus_tag="PLR47_05105"
     CDS             6562..>7770
                     /locus_tag="PLR47_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOG12354.1"
                     /translation="MDNIIGGKLVADALVERGVDYIFSLSGGHITPIYQFLENSPVTI
                     FDVRHEQAAVFMAEAWARINRKPAVAMVTAGPGFTNALTGIASTRLSNAPVILISGCV
                     GLDSVEKLDLQDMTQLPVIEPMVKKALVCYMPERIPEYIDMAFRAAMSGRPGPVYLEF
                     PCNILNAKVDMAAVKKPGTTLSSKPVDRENTRKVVDLLKAAKNPIVIGGSGLWYSDAE
                     KEILEFVEKIGIPIYTAGAGRGVVPDTHPLCFEGSLAIRPGSAMITLMTTDLVLFLGG
                     RLSLFYIFGEIFPSTAKFVQVDIAADEIGRNRTIDLGIVSDIKAFLKELNTVLDEQKI
                     GDTLKKQYSPWVDTVRAAHDAGKKDAKAMWECETLPMHPMRLAKEVNEFMNREDDIVV
                     ADGGDTSTWMG"
BASE COUNT         1625 a         2236 c         2232 g         1577 t
ORIGIN      
        1 ctcaaggaat ggttttacta cttttaccaa tgcacggagt gccgccgctg ctcggtgttc
       61 tgcccctacg ggatcgatca ggcggaaatt accatgatcg gccgcgaact gctcaacctg
      121 atcggctgca gcatcaactg ggtgctggag cccgcggcca actgcttccg gaccgggaac
      181 cacctgggca ttcagccggg cggcatcaag gacatgctgg agtttctggc ggacgacatc
      241 gaggatcgca ccggcgtccg cgtggatgtg ccaatcaacc aaaaaggcgc cgacattctt
      301 tttgtcacgc cgtccggtga ttattttgcc gatcccggaa cttacacttg catgggctac
      361 atgatgctct tccatgaaat cggcctcaac tacacgttca gcacctatgc ctctgaagga
      421 ggcaacttcg gcctgttcac ctcccacgag atggtcaaac gcctcaacgc caagatttac
      481 gcggaggcga aacgcctggg cgtcaaatgg attctgggag gagagtgcgg ccacatgtgg
      541 cgggtcattc accagtacat ggataccatg aacggtccgg cggactttct cgaagagccc
      601 gtctccccga ttacgggcac gcgctttgaa aatgcgcgct cgaccaaaat ggtgcacatt
      661 gtcgagttca cggcggacct gatcaaaaac ggaaaactga agctcgatcc gtcccgcaac
      721 gatcacatcc ggctgacatt tcatgattcg tgcaatccgg cgcgggccat ggggctgttt
      781 gaagagccgc gctacatcat tcaaaagtcc tgcaaccatt tctatgaaat ggccgaggat
      841 accattcggg aaaaaacatt ttgttgcggc ggcggggccg gactcggaaa tgatgagaat
      901 atggagatgc gaatgcgagg cggcatgccc cgtgccatgg ccgttcggga tgtcgttcga
      961 aagcacggcg tcaaccgcct gggctgcatc tgtgccatcg accgcgtcac gctcccccct
     1021 ctgatggact actgggtgcc tggcgtcacg gtcatgggcg ttcatgaact gctggccaac
     1081 gccctggtga tgaaaggcga aaaggagaga accaccgatt taagagggga ttcgatatga
     1141 gccccctcac cctgaccctc tcccacgagg ggagagggga aactacagat gccccctctt
     1201 ttgataccct gaagggtaca agcagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnagact cccctccctt gatgggaggg gcagggggag ggtgaaaata cggcgatggg
     1381 gttaggcgat agtggttagt ggatggtgaa tggtggatag ggttcagctc gcagctcgtc
     1441 gctgaacgta agcatattac ggaatgtatg aagatgaaac aacataaatg gggcttgata
     1501 cttctgggac tggcgatctt tgcaatcgcg gtcacgtttc ccttctggta cggcaaggga
     1561 aagacggccc cgcccgttct cagcctggag acgcctgaga tcgccggatt ggcggacaag
     1621 aagtgcgtcg aggacgccgt cttcatgcgc gccaaccaca tgaaaatgct ccaggcctgg
     1681 cgcgatgaag ccgtccgcga aggaaagcga gtgtacacgg caaatgacgg acggacgttc
     1741 gaaaagagcc tcaccggaag ctgccttcag tgccacagca acaaggagca tttctgcgac
     1801 cgctgccacc attacgtggg cgcggctccc tcctgctttg actgccacat cgttcccgcg
     1861 gaggtgaaaa aatgacgtcg agccgcagag actttctgaa aatcgcggga gcgtccgcac
     1921 tgacgatgat gacgcccgcg atctttgtcc cggcctgggc caaagacgtc atcgtgattc
     1981 ctccggggca gggccttgcg ctcaagaagg cgcttgcggc cggacgctgg gccatgacgg
     2041 tggatctgac aaagtgtacg gcggactgca ccgactgcat cgccgcctgt cacgcgattc
     2101 acaatgtacc ggacttcggc aatcccaagg acgaaatcaa atggatctgg aaagagtctt
     2161 ttgcctcggt ctttcccggc gaacagaacg cctattccgg cgagaagctg caaaagctcc
     2221 gtcctcccgt tttctgcaat aactgcgaaa atccgccctg cgtccgcgtg tgcccgaccg
     2281 gggctacgtt tcagcggccc gacggcattg tcatgatgga ctaccaccgc tgcatcggct
     2341 gccggctgtg catggccgcc tgcccctacg gcgcgcggag catgaattac cgcgacccga
     2401 gaccatgtat cgctaaaatc aatccggact ttccgacccg ttccaaaggc gtcgtggaaa
     2461 aatgcaattt ctgtgaggag cggctcgcca aaggaacgct gcccgcctgc gtggaggcgt
     2521 gccggcaaaa agcgcttacc ttcggcgacg cggagaatcc ccgctcggaa atccggcgtc
     2581 ttttgaagac gcgtttcagc ctgcaaaggc gtgtgcacct gggcaccggc ccgcagattt
     2641 actatttgtt atgaggacgc tatgctggaa aaagctttac acggcagcct gaggtaccgg
     2701 atctggatcg cctgtctgat ccttgtcggc tgcgccggat ttttcttcta cctgcgccag
     2761 ctcgattacg gtctgggcat cacggggatg agccgggacg tttcctgggg gctctatatc
     2821 gcccagttta cctttctggt gggcgtggcc gcctcggcgg tcatgctggt gctgccctac
     2881 tatctgcatg actacaaggc gttcggcaaa atcaccgtcc tgggcgagtt gctggccgtt
     2941 gccgccgtcg tcatgtgcat tcttttcgtt ttcgtcgatc tcggccggcc cgcccgcgtg
     3001 atgaacgtgc ttctgcatcc gcagccctcg tccattctct tctgggacat gattgtcctt
     3061 tccggctatt tgctcatcaa cctcgtgacc ggctgggtca tattggcctg cacaaaaaaa
     3121 gaaattccgc cgcccggctg gatcaaaccg gtcatttacc tgtcgattcc ctgggcgatc
     3181 agcattcata ccgtcaccgc tttcatttac gccggtctgt ccggccggag cttctggctc
     3241 accgcgatca tggcgccccg ctttctggcc tccgcttttg cctcggggcc tgcgctcctc
     3301 atcctggtct gcctgattct tcggcgctgg gggcgattcg acgcgggaga aaaagcaatc
     3361 caggcgctgg cgaagatcgt cgcctacgcg ctggccgtaa gcatcttctt tgtcggcgtg
     3421 gagatgttta cggtgtttta cagtcagatt ccggagcata tgcatcattt tcagtacctc
     3481 ttcatggggc tggaagggca tcacaacctt gttgcctgga tgtggatctt tgccgttcta
     3541 tcgattgccg cttttgtcat gctgatcctt cccggggttc gccggaatga ggtctggctg
     3601 gccctggcct gcatcgccgt ttttgtttcg ctctggatcg agaagggtct gggcctggtc
     3661 attaccggct ttattccgtc tccgctggga gcgatcaccg aataccaccc gacagggccg
     3721 gagatcacga tcactctggg cgtctgggcg gcgggatttt ttcttctcac ggtcttttac
     3781 aaaatttttg tccatgtgcg cggcggcacg gatggctccc cctgcggccc ggcggttgac
     3841 aaaatgatcc attaaaaaaa gaaattttcg gcaggagtca aaatagaata ctttggaggt
     3901 aaaaagatga aaaaagttaa aatgacaccc agcgaggcaa ttgtcgaaac cctgctggcc
     3961 gaaggcgtgg accacgtctg cggcatcgtg ggttcggcct ttatggacat gctggacctt
     4021 ttcccgacgg cgggaattaa attcattccg gtgcgtcacg aacaaagcgc ggcccacatg
     4081 gaagacgcct attgccgtat atcgggccgc tgcggggtcg tcacgggcca gaacggcccc
     4141 ggcatcacca acatggttac atcggtggcg gcggccaata tgggccacac acccctggtg
     4201 atcatttcgc cttcggcggg aacgccgacc atcggctggg acgggttcca ggaatgcgat
     4261 cagatgtcgg ttttcaagtc cgtcaccaaa gccgtcgttc gcgtccccca tccgaaacgg
     4321 gcggccgact gtctgcgcac cgctttccgc attgcctacg ccgaccgggg cgccgttctg
     4381 tatgatatac cccgcgatta tttctacggc gaactggaag aaaagattct aaagccttcg
     4441 cagtaccgtg tggacgcgcg cggctgcggc tccatggaac agctgaaccg ggcggccgag
     4501 cttctgtcca aagcgaaaaa cccggtcatc gtttccggac ggggcgtggt ggatgccgac
     4561 gcgctggata ttgttaaagc cattgccgaa catctgacgg caccggtagc ggtgacgtat
     4621 ctgcacaatg acgcgtttta cggaaatcat cccctgtccg tgggacccat cggctacatg
     4681 ggttccaagg cggccatgaa gacgctcgcc aaggccgacg tcatgctggc catcggcacg
     4741 cggctttccg tcttcggcac gctgccgcag tacgacatca attacttccc ggaaaacgcg
     4801 aagatcatcc aggtggacat caatcccaag cacatcgccc ggacgcatcc cattgaagtc
     4861 ggcatcatcg gcgacgcaaa agaagcctcc gcggaaattc tgaagcgcct gaaagcgatg
     4921 gacggcaggc gcaagccgga cgagaagcgc ctggcggccg tcgcccggga aagaaagaaa
     4981 tgggaaaagg aaattgttga tctggccatg gtggacggca atcccatgaa cccgcgccgt
     5041 gttcttctgg aaatgtcgcg ggcgttgccg gatgacgcca tcgtgaccac cgatatcgga
     5101 aatgtctctt cgacggccaa cagctacctg aaattcaacc atccgcgccg gcatatcgcg
     5161 gcgctgacct tcggcaacac gggctttgcc taccccgcgg cgctgggtgc gcagatcgcc
     5221 cgccccaaag cgccggtgat cgccatcatc ggcgacggcg cgtggggaat gagccttcat
     5281 gaagtcagca ccgccgtaga gcacaacctg cccgtgatcg cctgcgtctt tcgcaacatg
     5341 tggtggggcg cggaggccaa aaatcaggtg gacttctaca ataaccgttt tatcggcgtc
     5401 gatattccca acccggaaag ctttgtcccg gtagcgaaag cgatgggcgc ccatgcgatg
     5461 caggtcagcc gttcgcagga tattcgggaa gccttccaaa cctgcctgaa aagccgcaag
     5521 cccaccgtgc ttgaatttct ggtggacggc aagcagcttg cgccgccttt ccgcaaggac
     5581 gcgctggccc tgcccacgcg cttcctgtcc aagtacgccc atctggacca tcgccgctgg
     5641 gtcgatgaag gaggaagcga tgatttataa tgatttgaaa ggcaagacgg ccctggtcac
     5701 cggggccggc aaaaaaaccg gcatcggcta cgccatcgcc gagaagctgg cctcctgcgg
     5761 ctgcaatgtg attattgccg atctgggcgc gccgcccgcg ccggaaagcc ccctgcagcc
     5821 ggtcgtcagc gaagacatga aagccatcgc ttcggagctg gccaaagcat atgctgtccg
     5881 caccctggcc gtcgccgtcg atgtggcgga caaccaggcc atcggcgcaa tggtggagac
     5941 catcaaaaaa agcttcgatc atatcgacat tctctgcaac aacgcgggag cgtccttcgg
     6001 cgttcccaac acggtgctgg gttacgacga agcggcctgg atgaaaacga tcgatgtgaa
     6061 tctgttttcc gtttttcgca tgtcgcgggc cgttctgccc ctgatggcgg gcaaacctgc
     6121 ctcgatcatc aacacggcct cgcgggccgg caaggtgcct cccatcttca acagcgccta
     6181 cgccgtggcc aaggcgggcg tcatcatgct gaccaaagtc atggccaaag aactgggcgg
     6241 gggcgggatt cgggtcaacg cgatctgtcc gggacagatt gccacggatc tggaaaaatg
     6301 gcgattcggc ctggaggcgt cgtttttcgg cacaacgatc gaagagcggg aaaaggaaat
     6361 gtgcaagacc atcccgctgg gatatatcgg cctgccccgc gacgcgggcg acctggttgc
     6421 ttttctggcg tccgacgcct cccggtacat taccggacag gccgtcaacc ttgatggagg
     6481 ccagtgcatg gaactgtagg gaaccgtcgc gaccgttccc tgcgcacaat gaacaataaa
     6541 cctgttttag gaggatatat tatggacaac attatcggcg gcaagctcgt tgcggacgcc
     6601 ctggtcgaac ggggcgtgga ttacattttt tcgttgagcg gcgggcatat cacgcccatt
     6661 taccagtttc tggaaaactc acccgtcacc atctttgacg tgcgccatga gcaggcggcg
     6721 gtcttcatgg ccgaggcctg ggccaggatc aaccgcaaac ccgcggtggc gatggtcacg
     6781 gccggcccgg gattcaccaa cgcgctcacc ggcatcgcca gcacaaggct gtccaacgcg
     6841 ccggtgattc tgatttccgg atgcgtggga ctcgacagcg ttgaaaagct ggacctgcag
     6901 gacatgaccc agcttcccgt catcgaaccg atggtgaaaa aagcgctggt ctgctacatg
     6961 ccggaacgca ttcccgagta tatcgacatg gcctttcgcg cggccatgag cggacgaccg
     7021 ggaccggttt atctggagtt tccctgcaac attctgaacg ccaaagtgga tatggccgcg
     7081 gtcaagaagc ccggcacaac cctttcatcc aagccggttg accgcgaaaa cacccggaag
     7141 gttgtcgatc ttctcaaagc ggccaaaaat cccatcgtca tcggaggcag cggcctgtgg
     7201 tattcggacg cggaaaagga aatcctcgaa ttcgtcgaaa aaatcggcat tccgatttac
     7261 acggcgggcg cgggccgcgg cgtggtgccg gatacgcatc ccctgtgttt tgaaggctcc
     7321 ctggccatcc ggccgggttc ggccatgatc accctgatga cgaccgacct ggtgctcttc
     7381 ctgggcgggc ggttgagtct cttctacatc ttcggcgaaa ttttcccatc gacagccaaa
     7441 tttgttcagg tcgatatcgc cgccgacgag atcggccgca accgcacgat tgatctcggc
     7501 atcgtcagcg acatcaaggc gtttttgaaa gagctcaaca ccgtcctgga tgaacaaaaa
     7561 atcggcgaca cgctgaaaaa acagtattca ccctgggtgg ataccgtccg ggcggcgcat
     7621 gatgccggca aaaaagacgc caaagccatg tgggaatgcg agaccctgcc catgcatccc
     7681 atgcgtctgg ccaaagaggt caatgaattc atgaaccgcg aggatgacat tgtcgtcgcg
     7741 gacggcgggg atacgtccac ctggatgggc
//
LOCUS       DAOSIM010000086         7661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_8801_length_7661_cov_7.178280, whole genome shotgun sequence.
ACCESSION   DAOSIM010000086 DAOSIM010000000
VERSION     DAOSIM010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7661
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8801_length_7661_cov_7.178280"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PLR47_05110"
     CDS             complement(<1..561)
                     /locus_tag="PLR47_05110"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOG12355.1"
                     /translation="MKNRRQAQEAPEKDVRQRRPRLQATREPQRPRNLAERELRESEE
                     RLRSVVSRSPIPAFVIGKDHRLLYWNKALEQLTKIRAEDVIGTSQQWRAFYARKRPCM
                     ADLLVSRAQEKISKWYTGKYIRSKLLDEAYEAIDFFPELGHQGKWLRFTAAIIRNEAG
                     DLIGAIETLEDITRRKKAKEALLKAHR"
     gene            972..3062
                     /locus_tag="PLR47_05115"
     CDS             972..3062
                     /locus_tag="PLR47_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOG12356.1"
                     /translation="MEKFFTPRSMVVFGASVTKMNLGQIVLINNKQIGYPGEIYGVGS
                     QEGDINGVHVYTKVADLPCVPDVAIFLTPARTVPGLMEECGRKGITHVVIESGGFSEY
                     SHGHHTLEEDVLAVARKYGMKVIGPNCVGVINFDYKMMMPFAFAKEVPSGGTLGLITQ
                     SGGVGSSYLRTVAGFGITAGKFVSIGNKLQLDEADFLEYLIQDPRTKCIALYLEGFKR
                     GRKFFDLARNCDKPIILQKSNRSPASAKIAQSHTTALSGDDAVVDGAMRQAGVIRVDD
                     EVELVSAVQMVGMPLMKGKRVAVLSRSGGHAVLTADACAKYGFEMTPFPPSFIEKLKT
                     IYNTRVIAHQNPLDLGEIFDYTIFTDILEEALKLDDFDGVLFNHLYSSDFEAEMSRTF
                     LAGVKELVARYEKPVSLAMISDRDEILDLQKKQPFPVFVSPLEAVEALYASRSYYEKK
                     TACQKRGHLEKYDLDLKAVDRIRARCGKEKRIPLTDEALAVSAAAGLQPVKNLLLKDE
                     NIPSRITLRYPLAAKLLSRDASHKSDIGGVAVNLRSKKQLAEALAGMKSKIRNLKPSP
                     SIDGYLIQEMAPAGAECFVGGRRDPAFGPVIVVGLGGIFIEIFKDTSIRMAPVTPGEA
                     EGMIRELKAYPLLAGARGRGPADCQALQNVVCRISALLAACPEISEIDLNPVIVHPEG
                     QGVSLVDARVFFQK"
     gene            3147..6116
                     /locus_tag="PLR47_05120"
     CDS             3147..6116
                     /locus_tag="PLR47_05120"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HOG12357.1"
                     /translation="MRLLKKVLIAVLVFVVLIGLVGFLILPAVLKPVLAKKISEALHR
                     ETAIGQIKINPFDLSATIRGFKLADPGGHESFIAFQELHVNVDVLTSVFRRALILEEI
                     RLDQPHIGITRKADGTYNFSDLIPREETQPKEPAKPFLFSLNNIQITRGTADFHDTPN
                     KTNHSIRNLDLDVPFVSNIDYYMKHYVQPKFSAVVNGHTVSVAGKTQPFQASRESLLD
                     IDILDIDVPFYLQYVPVKMNFKLTRALMDIRLQLHFVMPQEKAPELKLTGRADLKNVA
                     VDDPRGNKILRLPSMAVKLASVRPLVPRVHLSEISLDGLQLAVRRNSKGEINLLNLVG
                     TPTKTKPEQTAPEKKKTDEPAGKKKELDLQVDALVLDGADITFTDEQPARPFRTRIHP
                     FRLAVSNFSLKKGNKAGIDLALVMDQKCRITAKGPVSLSPLGADLALGVKYLALRPLQ
                     SYIPDTVRMDITGGELSTTGAFALATDAKGEPSVKYAGQISVDHLATIDRARAHDFLK
                     WKQLRFQSLSAGYNPLFVNIREIALSDFFAKIVIHPGGATNIQHIFGVAKKETGRPDA
                     AAKAGEKIAAAKTPPPAVSAKPAQPPPDIKIGKVSFHGGTVDFADRNIRPNYAVTMLN
                     LQGSVTGLSSQQISRAKVDLKGNLGYGSPLEIAGTINPLAKDFFADIKLNFKDIEMSP
                     VTPYTSKYLGYPVTKGKLNFNVSYLVDQRKLSAENKIFFDQLTFGDRVESPEAIKAPV
                     PLAVSLLTDRNGQINLDIPLSGSLDDPKFKIWPIVWQVLKNIITKAVTAPFSLLSSLT
                     GGGEEMSFVEFNFGGADIPEAELKKISALSKALYDRPQLKLEVEGYVDATQDQEALKK
                     ARFNRLLKTQKRKEMIDRGETPATLEEITIGQAEYEKYLTLAYKAAKFSKPRTALGIA
                     KSLPPKEMEKLLFDNTIVTDADLEQLASRRAQAVREQLLKDGKVEAGRVFIVQPKAKT
                     PAKKEKVKDSRVDFKLK"
     gene            6149..7297
                     /locus_tag="PLR47_05125"
     CDS             6149..7297
                     /locus_tag="PLR47_05125"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HOG12358.1"
                     /translation="MIKLRAIMLLLLLGLLAGSCAPLLPVIVPVGKDQARCLPFFPDQ
                     DGWYGGDGAYSIALDKKRSLWLFGDTFVSKEKGRKDRIGMEVILGTTLAVSTCAGEQF
                     SIRYYLKKTNGKFASSFGPDQWLWPQDPFIADRVLYIPLLVIRPLPGPARPFHFEIAG
                     HTIARIRDYSAEDPNNWPVDYLDWTGAIAPGIEALAAASVVHGRFVYFYPLYRSGKDG
                     APVQGNLLARLPADHLENPAGHLEYWTLDGWQKKITPENLKMIFSPGLSELSVRYRMD
                     NEEWLAVYLSPEDRGRRLLYKTSRKPEGPWSPPAALIQSIDEVDPASPLYHPQTFCYA
                     GKEHPQFASGRDLVATYVCNSMENIDDQESFLRRNLFLYRPVVRLLRR"
     gene            complement(7322..>7661)
                     /locus_tag="PLR47_05130"
     CDS             complement(7322..>7661)
                     /locus_tag="PLR47_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG12359.1"
                     /translation="EQNLYLVRFILEKNGYTVHAAMDGQTGIDMAAALKPDMILLDIQ
                     LPVMDGYAVARNLRANPELAAIPIVAVTSHAMVGDRDKAMEVGCNGYIEKPINPDTFM
                     SEVERHLVVK"
BASE COUNT         1693 a         2270 c         2112 g         1586 t
ORIGIN      
        1 ccggtgcgcc ttgagcagcg cctccttggc cttcttgcgc cgggtgatgt cctcgagtgt
       61 ttcgatggcg ccgatcaggt cgccggcttc gttgcggata atcgccgccg taaagcgcag
      121 ccacttgcct tgatgaccga gctccggaaa aaagtcaatc gcctcgtagg cctcgtcgag
      181 cagtttggat cggatgtact tgcccgtgta ccacttggat attttttcct gggcgcggct
      241 gaccagcaaa tccgccatgc aggggcgctt tctcgcgtaa aacgcgcgcc actgctggga
      301 ggtgccgatg acgtcttcgg ccctgatctt ggtaagctgt tccagggcct tgttccagta
      361 aagaaggcga tgatccttgc cgatcacaaa cgcggggatc ggagaccggc tgacgacgct
      421 gcgaaggcgc tcttcgctct cccggagctc tctttccgcg aggttccgcg ggcgctgagg
      481 ctcccgggtc gcctgaaggc ggggacgccg ctgccgaaca tctttttccg gagcctcttg
      541 tgcctgccgc ctgttcttca tggcatcacc tttctttgaa gttggaaacc ggcctctacc
      601 cgcctttcgc caaccccatt gcgccgctgt gaagaatgga tctgataaaa tcacttggat
      661 gatgattata ttggctttac ccgggaagtc aaacgaatct ttgccgccgg cggacgggaa
      721 ccggcggccg gaaccgcgca gcctgatttt tcgccccggc ttccggcttc tctttgtcct
      781 tgacacaggc ccatccgttc tttataagag ccctgcccgg caaaaggaag tcaagttcat
      841 tttcctggtg tcattttggc gtttcacgct ttggctgatg gcgggcagca aggtgaaggc
      901 gtcgtttgct cagactggct caaaaacgcg gttgaatacc agcgttgccg ggcgaaagga
      961 gcaattcgat catggagaaa ttcttcaccc cccgcagcat ggttgttttc ggcgcgtccg
     1021 tcaccaaaat gaatctgggg caaattgtcc tcatcaacaa caaacaaatc ggctacccgg
     1081 gagaaatcta cggcgtcggg tcccaggaag gcgacatcaa cggcgttcac gtgtacacca
     1141 aagtcgcgga tctgccgtgc gttccggacg tggcgatttt cctgacgccc gcaaggaccg
     1201 ttcccggcct catggaggaa tgcggccgga agggcattac gcacgtggtg atcgaatcgg
     1261 ggggattcag cgaatactcg cacggccatc ataccctgga agaggacgtg ctggccgtcg
     1321 cgcgcaaata cggcatgaaa gtcatcggcc ccaactgcgt gggcgtgatc aattttgatt
     1381 ataaaatgat gatgcccttc gccttcgcca aggaagtccc ttccggcggc accctggggc
     1441 tcatcaccca gagcggcggt gtcggaagct cctatctgcg aacggtcgcg ggattcggaa
     1501 tcaccgccgg caagtttgtg tcgatcggca acaagctgca gctcgacgag gcggattttc
     1561 tcgaatacct gattcaggat ccccgcacga aatgcattgc gctgtatctg gaaggcttca
     1621 agcgcggacg caaatttttc gatctggccc gaaactgcga caaacccatc atccttcaga
     1681 aatccaaccg gtcgcccgcg agcgcgaaga tcgcccagtc gcacacaacg gcgctttccg
     1741 gcgatgacgc cgtggtggac ggagccatgc ggcaggccgg ggtcatccgc gtggacgacg
     1801 aagtggagct ggtcagcgcg gttcagatgg tcggaatgcc gctcatgaaa ggcaaacgcg
     1861 tggccgtgct gtcgcggtcc ggcggtcatg ccgtcctgac ggccgacgcc tgtgcaaaat
     1921 acggatttga aatgacgccc tttccgccgt cgttcatcga aaagctcaaa acgatttaca
     1981 acacccgcgt gatcgcccac cagaaccccc tggatctcgg cgaaatcttc gactacacga
     2041 tcttcacgga tattctggaa gaagccctca agctcgacga ctttgacggc gttcttttca
     2101 atcatctgta ttcctccgat tttgaggcgg aaatgtcccg gacctttctg gcgggcgtga
     2161 aagagcttgt cgcccgctat gaaaaacccg tcagcctggc gatgatatcc gatcgggatg
     2221 aaattcttga cctgcagaaa aagcagccat ttcccgtttt cgtgtctccg ctggaagcgg
     2281 tggaggccct gtacgcctcg cgatcctatt acgaaaagaa aactgcatgc caaaagcggg
     2341 gccatctgga aaagtatgac ctggatttga aagcggttga ccgcatccgc gcccgttgcg
     2401 gaaaggaaaa gcggattccg ctcacggacg aggcgcttgc ggtctccgcg gccgccgggc
     2461 tgcagcccgt gaaaaacctg ctgctcaaag atgaaaacat tccgtccaga atcacgctgc
     2521 gctacccgct ggccgccaag ctgctgtccc gggacgcctc tcacaagtcg gacatcggcg
     2581 gcgtcgccgt gaaccttcga agcaaaaaac agcttgccga agccctggcc ggcatgaaaa
     2641 gcaaaatcag aaacctgaag ccgtcgccct ccatcgacgg gtatctgatt caggaaatgg
     2701 cgcccgccgg cgccgaatgt tttgtcgggg gcaggcgtga tcccgctttc ggcccggtca
     2761 tcgtggttgg tctgggcggc attttcattg aaattttcaa agacacctcc atccggatgg
     2821 cgccggtaac cccgggggaa gccgaaggaa tgatccggga gctgaaagcc tatccgctgc
     2881 tggccggcgc aagaggcaga ggtcccgcgg attgtcaggc gctgcagaat gtggtatgca
     2941 gaatctccgc tctgctcgcc gcatgcccgg aaatttcgga aatcgacctg aatccggtca
     3001 tcgtgcatcc ggaaggccag ggcgtgagtc tggtggacgc ccgcgtcttt tttcagaaat
     3061 agccgcgggc ttcgggattg ccgacaagcc gcgcggaatg cggatcaaaa cggcctgccg
     3121 ccggcatctt tcgaaaggag cccttcatgc gtcttttgaa aaaagtcctg atcgctgtcc
     3181 tggttttcgt cgttctgatc ggccttgtcg gctttctgat tcttcccgcc gttctcaagc
     3241 cggtcctggc caaaaagatt tccgaagccc tgcaccggga aacggccatc gggcagatca
     3301 agatcaatcc ttttgatctt tccgcgacaa tccggggatt caaactggcc gatcccggcg
     3361 gtcatgaatc ttttattgcc tttcaagagc tgcacgtcaa cgtggatgtt ctgacgtccg
     3421 tctttcggcg cgcgctcatt ctggaggaaa tccgtctgga tcaaccccac atcggcatca
     3481 cccgaaaagc ggacggaacc tataatttct ccgacctgat tccgcgggag gaaacgcagc
     3541 cgaaggagcc cgccaaaccg tttctctttt ccctcaacaa cattcaaatc acccgcggca
     3601 cggcggattt tcacgacaca cccaacaaaa ccaaccactc gatccggaac ctggatctcg
     3661 atgttccgtt tgtttccaat atcgattatt atatgaagca ttacgtccag cccaaatttt
     3721 ccgccgtcgt caacggccat accgtctcgg ttgccggaaa gacccagccc tttcaggcat
     3781 cgcgggaaag cctgctggac atcgacattc tggatatcga tgttcccttt tacctgcagt
     3841 acgtgccggt gaaaatgaat ttcaagctga cccgggcgct catggatatt cggctgcaac
     3901 tgcattttgt catgcctcag gaaaaggctc ccgaactgaa gctgaccggc cgggccgatt
     3961 tgaaaaacgt ggcggtagat gatccgcggg gcaacaagat tctaaggctt ccttccatgg
     4021 cggtcaagct ggcttccgtc cggccgctgg ttcccagggt tcatctttcc gagatttcgc
     4081 ttgacggact tcaactggcc gtccggcgca attcgaaagg tgagatcaat ctgctgaatc
     4141 tggtcggaac gccaaccaaa acaaagccgg aacaaacggc gccggaaaag aaaaaaaccg
     4201 atgagccggc cggaaagaaa aaagagctgg atttgcaggt ggacgccttg gtgctcgatg
     4261 gggccgacat tacatttacg gacgagcagc cggcccggcc gtttcgcacg cggattcacc
     4321 cattccgcct ggccgtctca aatttttccc tgaagaaggg aaacaaggcc ggcatcgatc
     4381 tggcgctggt catggaccaa aaatgcagga tcaccgcaaa ggggccggtt agcctctcgc
     4441 cgctcggcgc cgatctggcg ctgggcgtaa aatatctggc gctccggccc ctccagtcct
     4501 atattccgga cacggttcgg atggacatca ccggcggcga gttgtccacg accggagcct
     4561 tcgcgctggc caccgacgcg aaaggcgaac ccagcgtcaa atatgccggc cagatctccg
     4621 tggatcatct cgcgacgatc gatcgggcgc gcgctcacga ttttctgaaa tggaaacagc
     4681 tccgttttca atctctttcc gccggctata atcccctgtt cgtgaacatt cgggaaattg
     4741 cgctcagcga tttcttcgcc aaaatcgtca tccacccggg cggcgccacc aatattcagc
     4801 acatcttcgg cgtggcgaaa aaggaaaccg gaaggcctga tgccgcggcg aaggccggag
     4861 aaaagatcgc ggccgccaag accccgccgc ccgctgtgag cgcaaaaccg gcgcagcccc
     4921 cgccggacat caaaatcgga aaggtcagct ttcacggggg cacggtggat ttcgccgacc
     4981 gcaacatccg gccgaactac gccgtgacca tgctcaatct tcagggaagc gtcacgggcc
     5041 tctcctcgca gcagatctcg cgtgcgaaag tcgatttgaa gggaaacctg ggctacggat
     5101 cgccgctgga aatcgcgggc accatcaacc cgctggccaa agatttcttc gccgatatca
     5161 agctcaactt caaagacatt gaaatgagcc cggtcacgcc ttacaccagc aaatacctgg
     5221 ggtatcccgt caccaagggc aaactcaatt tcaacgtgtc ctatctcgtc gatcagagaa
     5281 agctgtccgc ggaaaataaa atctttttcg atcagctcac cttcggcgac agggtggaaa
     5341 gccccgaggc catcaaggcg ccggtgccgc tggccgtttc ccttttgacc gaccggaacg
     5401 gacagatcaa cctcgacatt cccctgtccg gaagcctcga cgacccgaag ttcaaaatct
     5461 ggcccattgt ctggcaggtt ctcaaaaaca tcattaccaa agccgtcacg gcgccctttt
     5521 ctcttttgtc ttcgctgacc ggagggggcg aggaaatgag ctttgtcgaa ttcaacttcg
     5581 gcggcgcgga cattccggag gcggagctga aaaagatcag cgcgctttcc aaggcgctgt
     5641 atgaccgtcc ccaactgaag ctggaggttg aaggctatgt tgacgcaaca caggatcagg
     5701 aagctttgaa aaaagcaaga ttcaaccgtc tgctcaaaac gcagaagcgc aaggaaatga
     5761 tcgaccgcgg cgagactccc gctacgctcg aagaaattac aatcgggcag gccgaatacg
     5821 agaaatacct gaccctggcc tacaaggctg caaaattttc caagccgcgc acggcgctgg
     5881 gcatcgccaa aagccttccg cccaaagaaa tggaaaaact gctcttcgac aataccatcg
     5941 tcacggacgc cgatctggag cagcttgcct ccaggcgggc acaggcagtc cgcgaacagc
     6001 tcttaaaaga cggcaaggtg gaagcggggc gcgttttcat cgtccagccc aaagccaaaa
     6061 cgcccgcgaa gaaagaaaag gtaaaagaca gccgtgtgga ctttaaactg aaatagcggt
     6121 tgcgcggcga ttttcggatt cggaaaacat gataaaattg cgcgcgataa tgctccttct
     6181 gctcctcggc ctgctggcgg gctcctgtgc gccccttctg ccggttatcg ttcccgtcgg
     6241 gaaagaccag gcccgatgcc tgcccttctt ccccgatcag gacggctggt acggaggcga
     6301 cggcgcctac tccattgccc tggacaaaaa gcgctccctg tggctgttcg gcgacacctt
     6361 cgtttcgaaa gaaaaggggc ggaaggaccg gatcggcatg gaggtgattc tgggaacaac
     6421 gctggccgtt tccacctgcg ccggcgaaca attttccatc cggtattatc tgaagaaaac
     6481 aaacggcaaa ttcgcatcgt ccttcggccc agatcaatgg ctctggccgc aggacccgtt
     6541 catcgccgac cgggttttat acataccgct tctggtgatt aggcctcttc ccggaccggc
     6601 aaggcccttt cactttgaaa ttgccgggca caccatcgcc cggatcaggg attattcggc
     6661 ggaagatccc aacaactggc cggtggatta tctggactgg accggcgcga tcgctcccgg
     6721 catcgaggcc ctcgccgccg catccgtcgt tcacggccgt ttcgtctatt tttatccact
     6781 gtatcgttcc ggaaaggacg gcgctcccgt ccagggcaat cttttggcaa gacttcccgc
     6841 cgaccatctg gaaaatccgg ccggccacct ggagtactgg acgctcgacg gctggcaaaa
     6901 gaaaatcact ccggaaaacc tgaaaatgat tttttccccc ggtctttccg aactgagcgt
     6961 tcgctatcgc atggataatg aagaatggct ggccgtttac ctgtcgccgg aagacagagg
     7021 ccgccgcctg ctgtacaaaa catcccgaaa gccggaaggc ccgtggagcc cgcctgccgc
     7081 gcttatccaa tccattgatg aagtggaccc cgcaagcccg ctgtaccatc cgcagacgtt
     7141 ctgctacgcc ggcaaggaac acccccagtt cgcaagcggg agggacctcg tcgcaacgta
     7201 cgtctgcaat tcaatggaaa acatcgatga tcaggaaagt tttcttcgcc ggaatctttt
     7261 tttgtaccgg ccggtcgtcc ggctgctcag gcgctgacca tgaacggcgt cagtccgcct
     7321 gttatttgac caccagatgg cgctccactt ccgacatgaa cgtgtccgga ttgatgggct
     7381 tttcgatgta gccgttgcag ccgacctcca tggctttgtc gcggtcgccg accatggcgt
     7441 gcgacgtcac ggcaacaatg ggaatggcgg caagctcagg attggcgcgc agattccggg
     7501 cgacggcgta tccgtccatg acgggaagct gaatgtcgag cagaatcata tccggtttca
     7561 acgcggccgc catgtcgatg cccgtctgcc cgtccatcgc ggcatgcacc gtatagccgt
     7621 ttttctccag aataaaccgg accagataaa ggttttgttc a
//
LOCUS       DAOSIM010000087         7651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_8815_length_7651_cov_8.973670, whole genome shotgun sequence.
ACCESSION   DAOSIM010000087 DAOSIM010000000
VERSION     DAOSIM010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7651
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8815_length_7651_cov_8.973670"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2068)
                     /locus_tag="PLR47_05135"
     CDS             complement(<1..2068)
                     /locus_tag="PLR47_05135"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOG12360.1"
                     /translation="MKTNYVAAQADGSTLVSYGDTVVLVTAVSLKSVREGVDFLPLTV
                     DYQEMTFAAGKIPGGFFKREGRPNEREILTSRIIDRAIRPLFPKGYYSETQLVATVLS
                     VDKENDADVAAMIGASAALEISDIPFKGPIAGVRVGRIAGELVANASPERMQESEMNL
                     FLVGRKVTPGAAGRAYDVELVMMEGEAKEVGEDIIVDAIKFGLEAVRPVIDLQDQVRS
                     AIGKAKRPAQEAQPDEDLAARVRAEALPGLKEGYSLPRKLERYGKLGEVRDTVIKSIG
                     GDDAQLKKKVAAIIEHLESRILRDMIIQEKKRIDGRSSTDIRPISSEVGILPRAHGSA
                     LFNRGETQALAVLTLGTSSDEQRMDYIAGEERRAFLLHYNFPPYSVGEAKMLRSPGRR
                     EIGHGALARKALVPVLPPPEQFPYTIRIVSEILSSNGSSSMATVCGGILCLMDGGVPV
                     RDIVAGIAMGLLKEGDEVVILSDILGDEDHAGDMDFKVCGTEKGVTAMQMDIKIDGLT
                     EDILRKALAQAKAGRLHIIGKIRETMTAPRADISLYAPRITTIKVKEDQVRTVIGSGG
                     KNIRQIISETGVTIDVEDDGTVTIASADADAAARAVAMVKWLTEEAEVGKIYRGTVKK
                     IVDFGAFVEILPGTEGLLHISQIARERIAKVTDVLQEGDEVMVKVLEVDKSGKIRLSR
                     KEALGAE"
     gene            complement(2255..2521)
                     /gene="rpsO"
                     /locus_tag="PLR47_05140"
     CDS             complement(2255..2521)
                     /gene="rpsO"
                     /locus_tag="PLR47_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOG12361.1"
                     /translation="MLTLEKRKALIDDYRLHEKDTGSPEVQIALLSARIEYLTEHFKT
                     HQKDHHSRRGLLKLVGQRRRLLNYIKENDVERYRNIIKRLGLRK"
     gene            complement(2567..3505)
                     /gene="truB"
                     /locus_tag="PLR47_05145"
     CDS             complement(2567..3505)
                     /gene="truB"
                     /locus_tag="PLR47_05145"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOG12362.1"
                     /translation="MDGVVVMDKPAGLTSHDVVSRVKKILGAPKAGHTGTLDPLATGV
                     LPVCVGEATKLAPFLAGDDKTYRATMMLGLETDTQDTQGRETGRSDLVPSEEDIRLAL
                     GRLTGKIQQVPPAFSALKHKGRPLYQYARAGEFPQVAAREVEIFSLDVKSIGYPYVTF
                     EIACSKGTYIRTVCADAGRALGCGACLSGLRRLRSGFFTEDMAVPLEEDAAAARPALL
                     AKMLPMAQALPFAAAVEVSEPTAVRLRAGFQPEAGMLRENVLPFLAAGDMIKFITPDG
                     SLVALAEMQMPSKDIAKQEGKTRVARMVRVFNRTGP"
     gene            complement(3512..4462)
                     /locus_tag="PLR47_05150"
     CDS             complement(3512..4462)
                     /locus_tag="PLR47_05150"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOG12363.1"
                     /translation="MIKKIIAAISSGNRFLITAHIRLDGDALGSELALLGMLTNMGKE
                     AVVCNQDATPGHYRFLPGAEHIVHELVRPEAYDVAFILDCSELERVGNIAAAVSAVPT
                     LINIDHHVSNGGFCELQLLDSRASSTGELLHRLMREMRAPMTKDICMNLYAAILTDTG
                     GFRYSSTGHETLRAAGDLVEGGADPQWISENIYESDPPAKLQLLARVLETLEMDPELG
                     VASMAVTEGHLRQTGATMEHTDGFIDIIRAVKGINIAMLYTQTGEKQFKLSLRSKGRI
                     NVEKIARRFQGGGHVNAAACRIEGDLASIKSRVLDAVREA"
     gene            complement(4455..4814)
                     /gene="rbfA"
                     /locus_tag="PLR47_05155"
     CDS             complement(4455..4814)
                     /gene="rbfA"
                     /locus_tag="PLR47_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOG12364.1"
                     /translation="MNFKRADRVGELILAEMSDILLRSVKDPRLNAVTITAVKVTDDL
                     RNAKIYFVEMGKDELNEEILAGLTRARGFVRRELGHRLQLRFVPEILFVHDKSFGYGN
                     RIERLLDQIARQEEKHD"
     gene            complement(4804..5142)
                     /locus_tag="PLR47_05160"
     CDS             complement(4804..5142)
                     /locus_tag="PLR47_05160"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOG12365.1"
                     /translation="MIIGYGWISLRIPQSGSLKAKRSVLNKIIKRAQNEFNVSIAQVG
                     QLDDHQFARIGFAVTGNEQRFVEGKIDHLLHFVEDLNVADVLDAKVEMMAVSDFVEEA
                     AWVRGKYDEF"
     gene            complement(5155..>7651)
                     /gene="infB"
                     /locus_tag="PLR47_05165"
     CDS             complement(5155..>7651)
                     /gene="infB"
                     /locus_tag="PLR47_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOG12366.1"
                     /translation="TLEDHEIERIREDLQATSPRQVVEERIKSTVIRRRTVRTAVEES
                     PEEKEEKPEAEKAEREAAGAKKAKEAGEEKPEAQPASAAEEVERKTERAEAAVPESPA
                     PPEKEPAAEAKPVQPTPVAPPAKPKTPPVILPVRPPMMSRHKITRPDEKKEGPAKAPA
                     KPGEVIAPPPAEKIVKKEFEKPKKRGKGTVEVLIGDEKEVPRRKILERKIEKKLKRVD
                     DDRDVVIGKWREDRKTAPVRMKKTEITTPKAIKRRIRVGDTITTGELAKRMGVKAGEV
                     INKLMGMGVMATINQSIDFDIATLVASEFSFQVERAEVEFEDSMLKPEASGENLKPRA
                     PIVTIMGHVDHGKTSLLDAIRQTNVIEGEAGGITQAIGAYHVHINDRDIVFLDTPGHE
                     AFTAMRARGAQVTDIVVLVVAADDGVMDQTVEAINHSKVAGVPIIVAINKIDKPGADP
                     EKIKQALTEHGLLSEQWGGDTIFSEVSAKKKIGIEELLEMILLQADVLELKADPNTAA
                     RGIIIEAKLDRGRGPVATVLIQQGTLREGDAFVSKTESGRVRAMINDQGRRVKEAGPA
                     MPVEVIGFSSVPQTGAEFICVEDEKKARNIADYWIRKTREKELSASSKITLEQLYQKI
                     KEGVKDLNVIIKADVQGSIEAISDALHKLSTEDVQLKTIHSSTGAISETDVMLASASN
                     AIIIGFNVRPDARVSELAAQEGVEIKLYDIIYNVIADVRAAMEGLLEPEYKEVVQGRA
                     EVRELFKVPKVGIIAGCHVTDGKITRTAGVKLVRDSVAVFDGKILSLKRFKDDAREVL
                     AGFDCGIGIEGYNDIHVGDVIEAYTTETLERKL"
BASE COUNT         1383 a         2438 c         2049 g         1781 t
ORIGIN      
        1 cttccgcgcc cagcgcttct ttgcggctga ggcgaatctt tcccgactta tcgacttcca
       61 gaaccttgac catgacttcg tcgccttcct gcaggacatc cgtcaccttg gcaatgcgct
      121 ccctggcaat ctgcgagata tgcagcaaac cttccgttcc gggaagaatt tcgacaaacg
      181 cgccgaaatc gacgatcttc ttcaccgtcc cgcggtaaat cttgccgact tccgcttctt
      241 cggtgagcca tttgaccatc gccacggcgc gggcggccgc atcggcgtcg gcggacgcaa
      301 tcgtcacggt tccatcatct tccacgtcga tggtgacgcc ggtttcactg atgatctggc
      361 ggatattctt gccgccggag ccgatcacgg tgcgcacctg atcttccttc accttaatgg
      421 tggtaatccg cggggcgtac agagaaatat ccgcacgggg cgcggtcatc gtttcgcgga
      481 ttttgccgat gatatgcagg cggcccgcct tggcctgagc cagcgccttg cgcagaatat
      541 cctcggtcag gccgtcgatc ttgatgtcca tctgcatcgc ggtgacgcct ttttccgtgc
      601 cgcacacttt aaagtccatg tcgccggcat gatcctcgtc ccctaaaata tcggacagaa
      661 tcaccacctc gtcgccttcc ttcagcaatc ccatggcgat gcccgcgaca atgtcgcgca
      721 ccggcacgcc gccgtccatc aggcacagaa tgccgccgca aacggtagcc atggacgatg
      781 acccgttgga cgacagaatt tcggagacaa tccggatggt atagggaaac tgctccggcg
      841 gaggaagcac gggaaccagc gctttgcgcg ccagcgcgcc gtggccgatt tccctgcggc
      901 cgggcgagcg cagcatcttc gcctcaccaa cgctgtaagg ggggaaatta taatgcagca
      961 ggaaagcccg ccgttcttcg ccggcaatat agtccatccg ctgctcatcg gaagacgtcc
     1021 ccagggtgag gacggccagc gcctgcgttt cgccgcggtt gaagagggcc gaaccgtggg
     1081 cgcggggcaa aatcccgact tccgaactga tcgggcgaat gtccgttgaa gaccgcccgt
     1141 cgattctctt tttctcctga atgatcatat cccgaagaat ccgggattcg agatgctcga
     1201 tgatcgccgc aactttcttt ttgagctgag cgtcgtcgcc gccgatgctt ttgatcacgg
     1261 tgtcacgaac ttcgcccagc ttgccgtagc gttccagctt gcgcggcagg ctgtagcctt
     1321 ccttgagccc cggcagggct tcggcgcgaa cccgcgccgc cagatcctcg tccggctggg
     1381 cttcctgggc cggtcttttg gctttgccga tcgcagagcg cacctggtcc tgcagatcga
     1441 tcaccggacg gacggcttcc agaccgaatt taatggcatc cacaatgatg tcttcgccga
     1501 cctctttggc ttctccctcc atcatcacca gttccacgtc ataggcgcgg cccgcggcgc
     1561 ccggggtgac tttgcgtccc accaggaaga ggttcatttc gctttcctgc attctttccg
     1621 gcgaggcatt ggccaccagc tcgcctgcga tccggccgac gcgcacgccc gcaatcgggc
     1681 ctttgaacgg gatgtcggat atctccaaag cagccgacgc gccgatcatc gccgccacgt
     1741 ccgcgtcgtt ttccttatcc acggacagca cggtggccac gagctgcgtt tccgaataat
     1801 aacctttggg aaacaggggg cgaatcgccc ggtcgatgat gcgcgacgtt aaaatctccc
     1861 gctcgttggg gcgcccttcg cgcttgaaaa aaccgccggg gattttgccc gcggcaaacg
     1921 tcatttcctg ataatccacc gtgagcggca gaaaatcgac gccctctctc acgcttttca
     1981 gggataccgc cgtcaccagc accacggtgt ccccgtaaga gaccagcgtg gaaccgtccg
     2041 cctgcgcggc cacataattg gttttcactg caaaatttcg ccctgcgaaa tcagtgctgt
     2101 aattgtcact cattcacttt cctccacctt ccttgggttt atggtccgga acaggaaatt
     2161 caagggcgaa acgcaaaagc cgattgacgc agagatgaac aacatacgag gccaatcggt
     2221 gatgcttttg ccttccgccc ttgaatggat tgtgttactt cctgaggccc agacgcttga
     2281 ttatgtttct gtagcgttcc acatcgtttt ctttgatata attcagcaat cttcttctct
     2341 ggccgaccag cttcaacagg ccgcgccggg aatggtgatc tttctggtgc gtcttgaaat
     2401 gctcggtcag gtattcgatt ctcgcgctca gaagggcaat ctgcacctcg ggcgaaccgg
     2461 tatccttctc gtgaagacgg taatcatcga ttaatgcttt cctcttttcc aaagttaaca
     2521 cgtcaattcc tccctatcaa aataatatcc tttttatgtt ccgggctcac gggcctgtcc
     2581 ggttgaatac cctgaccatt ctggccaccc gggttttccc ttcctgtttg gcgatatcct
     2641 tcgacggcat ttgcatttcc gccagcgcca ccagagagcc gtccggagta atgaacttta
     2701 tcatatctcc cgccgcaagg aaaggaagaa cattttcccg cagcattccg gcctccggct
     2761 gaaatccggc ccgcagcctc accgccgtcg gttcgctgac ttcaaccgcc gcggcaaaag
     2821 gcagcgcctg cgccatcggc agcatctttg ccaggagcgc cggcctggcc gccgccgcgt
     2881 cctcctccag aggcacggcc atgtcttcgg tgaagaagcc gctcctgagc cttcgcaatc
     2941 cggacaaaca cgcgccgcag cccagcgcgc ggccggcatc ggcgcagacc gttcgaatgt
     3001 aggtgccctt cgagcaggcg atttcaaagg tcacgtacgg atagccgatg ctcttcacgt
     3061 cgagcgaaaa gatttccact tcgcgcgccg cgacctgtgg aaattctccg gcccgcgcgt
     3121 actggtacag gggcctgccc ttgtgcttca gcgccgaaaa agccggcgga acctgctgaa
     3181 tcttccccgt cagtcttccc agcgccagcc ggatgtcctc ttcggaggga accagatccg
     3241 acctgccggt ttctctcccc tgcgtgtcct gcgtatccgt ctcgaggccc agcatcatgg
     3301 tcgcccggta ggttttgtcg tcccccgcca aaaacggggc cagtttggtc gcttcgccca
     3361 cgcataccgg caaaacaccg gtggccaggg gatcgagcgt tccggtatgc cccgccttcg
     3421 gcgcgcccag gatcttcttg acccggctta caacgtcgtg ggacgtcagc cccgccggtt
     3481 tgtccatcac cacgaccccg tccataaacg cctatgcttc cctgacggca tcgagcacac
     3541 gggacttgat cgatgccaga tcgccttcga tccggcaggc cgccgcgttc acgtgccctc
     3601 cgccttggaa gcggcgggcg attttttcca cattgatccg gcctttggac cgcaggctca
     3661 gcttgaactg tttttcgccc gtctgcgtgt agagcatcgc gatattgatc cccttgaccg
     3721 cgcggataat atcgataaag ccgtccgtat gctccatggt cgcgccggtt tgccgcagat
     3781 gaccttccgt gacggccatc gacgcgaccc cgagctccgg atccatctcc agcgtttcca
     3841 gaacgcgggc cagaagctga agtttggccg gcggatcgct ttcgtaaata ttttcggaaa
     3901 tccactgcgg atcggcgccg ccttccacca ggtcgcctgc cgccctgagc gtctcgtggc
     3961 cggtgctgga atagcggaaa ccgcccgtgt ccgtcaaaat cgccgcgtag agattcatgc
     4021 agatgtcctt cgtcatcggc gcgcgcattt cgcgcatcaa acggtgaagg agttcccccg
     4081 tggaactggc tctcgaatcc aaaagctgca attcgcaaaa tccgccgttg gaaacatgat
     4141 ggtcgatatt gatcagggtc ggaacggcgg acaccgccgc cgcgatgttc cccacgcgct
     4201 ccagttccga acaatccagg ataaaagcga catcgtaggc ttccggacgg accagctcgt
     4261 ggacgatatg ctccgcgccg ggcagaaaac ggtagtggcc gggcgtggca tcctggttgc
     4321 agacgaccgc ttccttgccc atattcgtga gcatgcccaa aagcgccagc tcggagccca
     4381 gggcgtcccc gtccagccgg atatgggccg taatgagaaa ccgatttccc gaagaaatgg
     4441 ctgcaataat tttcttaatc atgcttttct tcctgcctgg cgatttgatc cagcagccgc
     4501 tcgatgcggt tgccgtaacc gaaggatttg tcgtgcacaa acagaatctc gggcacaaaa
     4561 cgaagctgca ggcgatggcc cagctcccgg cgcacaaatc ccctggcccg ggtcaatccg
     4621 gccagaattt cctcattcaa ttcatccttg cccatctcga caaagtaaat tttcgcgttt
     4681 cgcagatcgt cggtcacctt gaccgccgtg atcgtcaccg cgttcaggcg gggatctttt
     4741 acggacctca gcagaatgtc ggacatttcc gccagaatca gctcgccgac acggtccgcc
     4801 cttttaaaat tcatcgtact ttcccctgac ccacgcggct tcttcaacaa aatcggaaac
     4861 cgccatcatt tccaccttcg cgtccagaac atccgccacg ttcaaatctt cgacaaaatg
     4921 cagcaggtga tcgatcttgc cctcgacaaa gcgctgctcg ttgccggtta cggcaaaccc
     4981 gatccgggcg aactggtgat catccagttg gcccacctga gcgatggaaa cgttgaattc
     5041 attctgggcg cgcttgatga ttttattcaa aacgctccgc ttggccttta aagaaccgga
     5101 ttgcggaatc cgcaatgaaa tccatccgta gccgataatc atcgccgccc ttttctacaa
     5161 ttttctctcc agcgtctccg tggtatacgc ctcgatcaca tcccccacgt ggatgtcgtt
     5221 atagccttca atgccgatgc cgcaatcgaa gccggccagc acctcacggg cgtcgtcctt
     5281 gaaccgcttc agggaaagga ttttcccgtc aaaaaccgcc acgctgtcgc gaaccagttt
     5341 gacgcccgcc gtgcgggtaa ttttgccgtc cgtcacgtgg cagccggcga tgatgccgac
     5401 cttgggaacc ttaaagagtt cgcggacttc ggcgcgtccc tggaccacct ctttgtactc
     5461 cggctccaga agcccttcca tggccgcccg tacgtcggca atcacgttgt aaataatgtc
     5521 gtagagcttg atttccacgc cttcctgcgc cgccagctca ctcacccggg catccggacg
     5581 cacgttgaat ccgataatga tcgcgttgga ggcggaggcc agcatgacat ccgtttcgct
     5641 gatcgccccc gtggaactgt gaatggtttt gagctgaaca tcttcggtgg agagtttatg
     5701 aagcgcgtcg gagatcgctt cgatcgaacc ctgcacgtcg gctttgataa ttacgttcag
     5761 atctttcacg ccctctttga tcttctggta cagctgctcc agcgtgattt tggaggaagc
     5821 ggaaagctct ttttccctgg tcttgcgaat ccagtaatcc gcgatgttgc gggctttctt
     5881 ctcatcttcc acgcagataa attcggcgcc ggtctggggc acgctggaaa aaccgatcac
     5941 ttccaccggc atggccggcc cggcttcctt cacgcggcgt ccctgatcgt tgatcatcgc
     6001 ccggacgcgg ccggattcgg tcttggaaac aaaggcgtct ccctcgcgca gcgttccctg
     6061 ctgaatcagc acggtagcca cgggaccgcg gccgcggtcc agcttggctt caatgatgat
     6121 gccccgcgca gccgtgttcg gatcggcctt gagctccagc acgtccgcct gcagaagaat
     6181 catctccaga agctcctcga tgccgatttt cttcttggcc gaaacttcgc tgaaaatcgt
     6241 gtcgcctccc cactgctcgg acaagagtcc gtgctcggtc agcgcctgct tgatcttttc
     6301 gggatccgca cccggtttgt cgatcttatt gatggccacg atgatgggca cgcccgccac
     6361 tttggagtgg ttgatggctt ccaccgtctg atccatcacg ccgtcgtcgg cggccaccac
     6421 gagcaccacg atgtccgtca cctgggcgcc gcgggcgcgc atggccgtga aggcttcatg
     6481 gccgggcgta tccagaaaga cgatgtcgcg gtcgttgatg tgaacatgat aagcgccgat
     6541 ggcctgcgtg atgccgcccg cttcgccttc gatcacgttg gtctgacgga tggcgtccag
     6601 aagcgacgtc ttgccgtgat ccacgtggcc cataatggtg acgatcgggg cccgcggctt
     6661 gagattttct ccggaagcct cgggcttcag catcgaatct tcaaattcga cctccgcccg
     6721 ctccacctga aagctgaatt cggaggccac cagcgtggca atatcaaagt ctatcgactg
     6781 gttgatggtg gccatcacgc ccatgcccat gagtttgttg atgacctcgc ccgccttgac
     6841 gcccattctc ttggccagct cgccggtcgt aatcgtgtct cccacgcgga tgcgcctttt
     6901 gatggctttg ggcgtcgtga tttcggtctt cttcatcctg acgggcgcgg ttttccggtc
     6961 ttcgcgccat ttgccgatta cgacatcccg gtcgtcatcc acccttttga gtttcttttc
     7021 gattttccgc tccagaatct tgcggcgcgg aacctctttc tcgtcgccga tcaggacttc
     7081 aaccgttccc tttcctcttt tcttgggctt ctcgaattcc tttttgacga ttttctccgc
     7141 cggcgggggc gcgatgactt cgccgggttt ggcgggggct ttggccggac cttctttttt
     7201 ctcgtcgggc cgggtgattt tgtggcggga catcatcggc ggcctgaccg gaagaatcac
     7261 cggaggcgtt ttgggcttcg ccgggggcgc aaccggcgtc ggctgcacgg gctttgcttc
     7321 cgctgccggc tctttttccg gcggggccgg gctttcggga accgccgctt ccgcccgctc
     7381 cgttttccgt tcaacctctt cggcggccga cgcgggctgc gcctcaggct tttcttcccc
     7441 ggcttccttt gcttttttgg ctcccgccgc ttcgcgctcg gccttttcgg cttccggttt
     7501 ttcttctttt tcttccgggg attcttcgac cgccgtccgg accgtgcggc ggcggatcac
     7561 ggtggatttg attctttctt ccacgacctg gcggggcgag gtggcctgca ggtcttccct
     7621 gatcctttcg atttcatgat cttccagggt g
//
LOCUS       DAOSIM010000088         7611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_8869_length_7611_cov_7.549629, whole genome shotgun sequence.
ACCESSION   DAOSIM010000088 DAOSIM010000000
VERSION     DAOSIM010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8869_length_7611_cov_7.549629"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="PLR47_05170"
     CDS             <1..928
                     /locus_tag="PLR47_05170"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG12367.1"
                     /translation="CISCLWCFESFLTNAAKGETLECAVNPRLGRERETAEPLKNGAG
                     RVVVIIGAGPAGLSAAGILGQRGFKPIVLEKNAAVGGQLQLANKPPKKEKINWCFADL
                     EHAALQNGAEIRLNAEATPASVKALAPYAVIIATGAKAVVPPVEGVHLPHVCTVTEIL
                     NGSVRLKNQRVAVIGSGMTGLETAEKLAEEGNRVLVAEMMDRIAPGVHPQHLDDILPR
                     LKAYSADFITGHKLAKISAGVVFLESVAGGKRREEKADAVVLSVGVQSNSALAAALQP
                     HFPRLRVIGDARRVGRIPQAVRDGFDTAWNLM"
     gene            962..1492
                     /locus_tag="PLR47_05175"
     CDS             962..1492
                     /locus_tag="PLR47_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12368.1"
                     /translation="MSIIKDVQSGKKPLKRAYVAIFLWFTGRAIQAAAKVDSEVKKEF
                     EKLPEDFTFSLGCLPCGPWMVVGREKGVVKYLGGDSRGRPVQVRLAIKSLDAGFLILS
                     FQESTALATARNRLITDGEIPQALAIIRILDIVEVYLLPKLIASLAVKRYPSWWSFGR
                     KLGGRIGVYTRTLLGI"
     gene            1508..2674
                     /locus_tag="PLR47_05180"
     CDS             1508..2674
                     /locus_tag="PLR47_05180"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOG12369.1"
                     /translation="MNSAAVFDFTCPVKTNSGNKALEHLPVELAGLNAVNPLIVANAR
                     AVGPKAVRTLMNAFGDSKMTLGLFEGVTEAADLGVVDHLKNICLEKKYDAFIALGGGK
                     AADVAKVLNLAVSLKTPDARQLSPKTPVRKRLLPLVVVPTAGADGLETSGFAHLNRMI
                     FSSEFLAPGLAVLDSRLARARKGILMAATGLAALGRAVESHVSADRNPFQKAYSFAAV
                     RFVRENLPAAVADPGNKKAALAVLNAAAMSGCAFSNAASGRLHKLGQVFYELLGIPQG
                     VIMGMCLSRVLEDGFREGSCDPAALLQPLAGDDAFARTPAGKRAEAALALLNRFLEEL
                     RGALGKDLPKSLSEAGVSGYRKEEILDVLGKDEDGHYLRGVVEGMAGGKRERKG"
     gene            2680..3927
                     /locus_tag="PLR47_05185"
     CDS             2680..3927
                     /locus_tag="PLR47_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOG12370.1"
                     /translation="MLLPDYYEFCCRVKIVAGHDALEKIPDLLIERGAKKPLVITDKG
                     VAGAGLIDIVSRALGDRVQADLIADDVPPDSDLRVVNRLARLYRDNACDAIIAVGGGS
                     VMDTAKGINIVVSENAEDLMAFSGVGALKRPLKPLVAVPTTAGTGSEVTQVAVIKDHE
                     KHLKMAFGSYFLLPDISILDSRMTLTMPPFITAMTGMDAMSHAVEAYYCLQKNPLSDA
                     HALSAVGMIYRHLPLVVKNPDDREGRLAMAVAACLAGIAFSNSMVGLVHNLGHATGSV
                     CGVPHGLCMAILLPYGLEYNLHKMADVMAELLLPMAGEQVYLATPAEQRAQKVVSLIR
                     GLNEDLHAITGGRHARCLQEIKDREGKPSVPRDVLPQIAAAALLDGASIYNPEDTDYE
                     DNLMVLEAAWEGKPLDRSRVKKG"
     gene            3947..5893
                     /locus_tag="PLR47_05190"
     CDS             3947..5893
                     /locus_tag="PLR47_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOG12371.1"
                     /translation="MIKPYMGKILMVDLSSRTMTEEEIPDRVYEQYLSGMGLGAYILY
                     HRMPAGADPLGPDNILGFCSGLLTGTGSLFSGRWTLVGKSPLTGCWGDANCGGTFSPA
                     IKHSGYDAIFFTGISSGPVYLYVKNGKASLKDAADLWGRDAVETEEALKKQYGPKAAV
                     AVIGPAGEKLSLISGVCSDKGRIAARSGLGAVMGAKRLKAVVLDGVQRIGARDPQAMK
                     RLSQHCLKWINFPLPLPGWMLGYVGAFLRITPVVFPQDGILYNSILRKWGTGGMNQTS
                     IEMGDSPLKNWDGTNRDLPMSKSRTINPDIIRSMEIVKYHCYSCPVGCGGICAAKGKY
                     PHTHKPEYESVLALGGLCMNEDLDSIFYLNELLNRAGMDTISAGATAAFAVECYEKGI
                     LTREDTGGLELKWGNTEAIVELIRKMANREGIGDLLADGSKIAAQRIGKGSEACTVHA
                     GGQEPAMHDGRCDPGFNVHYSLEPTPGRHTLGAHLYYEMFQLWKRVKGIPKPLPLFIK
                     NSKFRVDGEKARMSAACSKFMSLANGAGMCMFGLFVGIKRVPTFDWLNAATGWNRTPE
                     EYMTVGERIQTLKQAFNVKHGVEPKNNFISSRALGTIPQAEGANKGRSVDIEKLASGY
                     WEQFGWDAATGKPKEETLRKLEIQ"
     gene            5904..6356
                     /locus_tag="PLR47_05195"
     CDS             5904..6356
                     /locus_tag="PLR47_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG12372.1"
                     /translation="MAYTITEKCNGCGACARLCPAGAITGEKKKLHAIEAVLCIECGA
                     CGRICPQAAVLDAAGAPCAMIKRSEWPKPEILLKACIACTVCVDACPVGCLAMSDLSR
                     DKGVDAFPHLRDSKACIGCGFCSRECPADALVMKKPVPAAQTVAAESA"
     gene            6353..7444
                     /locus_tag="PLR47_05200"
     CDS             6353..7444
                     /locus_tag="PLR47_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HOG12373.1"
                     /translation="MSIETIPRYPIDKIISGGQTGADRAALDFAIARNLPYGGWLPRG
                     RKTEDGALDLKYRLQEMPTTDYAKRTEQNVIDADGTVIVSHGFPVAGSALTLEFTRRH
                     DRPCLHIDLKEISPEKAAEVLVSWLGENRIRVLNVAGPRAGRDPAIYPAVMDLLERSI
                     ENAGPVDVVLFDFGGVLAEEGWKDGLQAIAHANGLKAVELLLIAADTVYETGYILGKG
                     SEISYWDAMRRKTGIRGDYAQLRDEILSRFILRENMIDLVKRLKADNLVVGILSDQTD
                     MLDQLNARMNFFQWFDYVFNSYHMGKGKRDATLFDDIAQRLKTLPGRILFIDDDPGNV
                     ERAKQRGWKAILYSDENVFQSELDRLLSA"
BASE COUNT         1591 a         2222 c         2343 g         1455 t
ORIGIN      
        1 ctgcatcagc tgcctgtggt gctttgaatc ctttctgacc aatgccgcca aaggcgagac
       61 gctggaatgc gccgtcaatc cgcggctggg ccgtgagcgc gagaccgccg agccgctgaa
      121 gaacggagcc ggccgcgtgg tggtcatcat cggtgcgggg cccgccggac tttccgcggc
      181 cgggattctg ggccagcgcg gattcaaacc catcgttctg gaaaagaacg ccgccgtcgg
      241 cggccagctg cagcttgcca acaagcctcc caaaaaggaa aagatcaact ggtgctttgc
      301 cgatctcgag catgccgccc tgcaaaacgg ggctgaaatc cgtctgaatg ccgaggcaac
      361 gcccgcatcc gtgaaggctc tcgctcccta tgccgtcatc attgccaccg gagcgaaagc
      421 cgtcgtcccg cccgtcgaag gcgttcatct tccgcatgtc tgcaccgtca ccgaaatcct
      481 gaacggctcc gtccggctta aaaatcagcg ggtcgccgtg atcggctcgg ggatgaccgg
      541 cctggaaacg gcggagaagc tggcggagga aggcaaccgc gtcctggtgg cggaaatgat
      601 ggaccggatc gcgccgggcg tccatccgca gcatctggac gatatccttc cgcgcctgaa
      661 agcctattcc gcggatttca tcaccggcca caagcttgcc aaaatttcgg ccggcgtcgt
      721 ttttctggaa agcgttgccg gcggaaaacg ccgcgaagaa aaggccgatg ccgttgttct
      781 ttccgtcggc gtgcaaagca acagcgcgct ggccgcggcg cttcaacctc actttcccag
      841 actccgcgtc atcggcgatg ctcgcagggt cggccgcatt ccccaagccg tgcgggatgg
      901 ttttgacaca gcctggaatt tgatgtaaaa gaaaaaaccc attagacagg aggatcggat
      961 catgagcatc atcaaggacg ttcaatccgg gaaaaagccg ctcaagcggg cgtatgtagc
     1021 catttttctg tggttcaccg gccgcgccat tcaggcggcg gcaaaggtcg attccgaggt
     1081 caaaaaagaa ttcgaaaaac ttcccgagga tttcaccttt tctctgggct gtctgccctg
     1141 cggcccctgg atggtggtgg gccgggaaaa gggcgtcgtg aagtatctgg gcggagattc
     1201 ccgcggacgg cccgtgcagg ttcgcctggc catcaagagc ctggacgcgg gctttctgat
     1261 tctctccttt caggaaagca cggcgctcgc cacggcgcgc aaccggctga tcacggacgg
     1321 ggaaattccc caggcgctgg ccattatccg cattctcgat attgtggaag tttaccttct
     1381 gcccaagctc atcgcctctc tggccgtgaa gcggtatcct tcctggtggt cgttcggccg
     1441 caaactcggc ggccgcatcg gtgtttacac ccgcacgctg ctgggcatct aacgaaagga
     1501 atacgatatg aattccgcag ccgtttttga ttttacctgc cccgtcaaga ccaattccgg
     1561 aaacaaggcg ctggagcacc tgcccgtgga gctggccgga ctcaatgccg tgaaccctct
     1621 gatcgtcgcc aacgcgcggg cggtcggccc caaagccgtc cggacgctga tgaacgcatt
     1681 cggcgattcg aaaatgacgc tgggcctttt tgaaggcgtc accgaagcag ccgatctcgg
     1741 cgtcgtggat catttgaaaa acatctgcct ggaaaaaaaa tacgatgcct tcatcgcgct
     1801 gggcgggggg aaagccgccg atgtcgccaa ggtgctgaat ctggcggttt cgctgaaaac
     1861 gccggacgcg cggcagcttt cgcccaagac gcccgtccgc aagcgcctgc tgccgctggt
     1921 ggtcgtgccg acggccggag cggacggcct ggaaacctcg ggctttgccc atctgaaccg
     1981 gatgattttt tcctccgaat ttctcgcgcc cggcctggcc gtgctggatt cgcgcctggc
     2041 gagagcaaga aaaggaatcc tgatggccgc gacgggcctt gccgcgctgg gccgcgccgt
     2101 ggaatcgcac gtcagcgccg acagaaaccc tttccagaaa gcctattcct ttgccgccgt
     2161 ccgctttgtc cgggaaaacc ttccggccgc ggtcgccgat cccgggaata aaaaggcggc
     2221 gcttgccgtg ctcaacgccg cggcgatgtc cggctgcgcc ttttccaatg cggcctccgg
     2281 caggctccac aaactcggtc aggtgtttta cgaactgctg ggcattcccc agggcgtgat
     2341 catgggcatg tgcctgtccc gcgttctgga agatggcttt cgggaaggct cctgtgaccc
     2401 ggcggctctt cttcaacccc tggccggaga cgacgcgttt gcgcgaacgc ccgccggaaa
     2461 gagggcggag gccgctcttg ccctcctgaa ccgttttctg gaagagcttc ggggcgctct
     2521 gggcaaggat ctgccgaaga gcctttcgga ggcgggagtg tccggctacc ggaaggagga
     2581 aattctggat gtgctgggaa aagacgaaga cggccattac ctgcgcggcg tcgtggaagg
     2641 gatggccgga ggaaaacggg aaaggaaggg gtgattccga tgcttttgcc ggattattat
     2701 gagttctgct gtcgtgtgaa aattgtcgcg ggtcatgacg ccctggaaaa aataccggat
     2761 ctgctcatcg agcgcggcgc aaagaaacca ctcgtcatca cggacaaggg agtggcgggc
     2821 gcgggtctca tcgatatcgt ttcccgggcg ctcggggacc gcgtgcaggc ggatctgatc
     2881 gccgacgatg ttccccccga ttcggatctc cgggtcgtca accggctggc gcgcctttac
     2941 cgggacaacg cctgcgacgc gatcatcgcc gtcggcggcg gttccgtcat ggacacggcc
     3001 aaggggatca acatcgttgt ttcggaaaac gcggaagacc tgatggcctt cagcggcgtc
     3061 ggcgcgctca agcgccccct gaagcccctg gtggccgtcc cgaccaccgc cggcacgggg
     3121 tcggaagtca cgcaggtggc cgttatcaag gatcacgaga agcatttgaa gatggccttt
     3181 gggagttatt ttctgctgcc cgacatttcc atcctcgatt cccgaatgac cctgacgatg
     3241 ccgcctttca tcacggccat gaccggcatg gacgccatga gccacgccgt ggaagcctac
     3301 tattgccttc agaaaaatcc gctctccgac gcccacgcgc tttccgccgt cggcatgatt
     3361 taccggcacc tgccgctggt cgtgaaaaat ccggatgatc gcgaaggccg cctggccatg
     3421 gcggtcgccg cctgcctggc gggcatcgcc ttttccaatt ccatggtcgg actggttcac
     3481 aatctcggcc atgcgaccgg ctccgtctgc ggcgttcccc atggcctctg catggccatc
     3541 cttctgccct acggcctgga gtacaacctg cacaaaatgg cggacgtcat ggcggagctc
     3601 ctgcttccca tggcgggaga gcaggtgtat ctggccacgc ctgcggagca gagagcccaa
     3661 aaagtcgtat ccctgatccg cgggctcaat gaagacctcc acgccatcac cggcggacgg
     3721 cacgccagat gcctgcagga gatcaaagac cgcgaaggga agccgtccgt cccccgggac
     3781 gtgctgccgc aaatcgccgc cgctgcgctg ctcgacggag cgtctattta caatccggaa
     3841 gacacggact acgaggacaa cctgatggtg ctggaagcgg cctgggaagg gaagcctctg
     3901 gacaggtctc gcgtaaaaaa aggctgatga acgaaaggga gcagtcatga taaaacctta
     3961 catgggaaaa atcctcatgg tggatttgtc cagccggacc atgacggaag aagaaatccc
     4021 cgaccgggtg tatgaacagt atctgtccgg gatggggctg ggcgcctata tcctctacca
     4081 tcgcatgccg gccggcgccg acccgctggg tccggacaat attctcggtt tctgctcggg
     4141 attgctcacg ggaaccggca gcctgttttc cggccgctgg acgcttgtgg ggaaatcgcc
     4201 gctgaccggg tgctggggcg acgcgaactg cggcggcacc ttttctccgg ccatcaagca
     4261 ttccggctat gacgccattt tcttcaccgg catcagttcc ggccccgttt atctgtatgt
     4321 gaaaaacggc aaagcttctc tgaaagacgc ggccgatctc tgggggcggg atgccgtcga
     4381 gacggaagaa gcgctcaaaa agcagtacgg ccccaaagcg gccgtggccg tcatcggccc
     4441 cgccggagag aagctttcgc tgatttccgg cgtttgcagc gacaagggac ggatcgccgc
     4501 ccggtcgggt ctgggcgccg tcatgggagc caaaaggctc aaagcggtgg tgctcgacgg
     4561 cgttcaaagg atcggcgccc gcgatcccca ggccatgaaa cggctgagcc aacattgtct
     4621 gaaatggatt aatttccccc tgcccctgcc gggctggatg ctgggctatg tcggcgcctt
     4681 tttgcgcatt acgcccgttg tgtttcccca ggacggcatt ttgtacaatt ccatcctgcg
     4741 caaatggggc acggggggca tgaaccagac ctccattgaa atgggggatt cgcccctgaa
     4801 gaactgggac ggcaccaatc gcgatcttcc catgtcgaag tcccggacca tcaatccgga
     4861 cattatcagg agcatggaaa tcgtgaagta ccactgctat tcatgcccag tgggatgcgg
     4921 cggcatctgc gccgccaaag gaaaatatcc ccacactcac aaacccgaat atgaaagcgt
     4981 cctggcgctg ggcggcctgt gcatgaacga ggacctggac agtatctttt acttgaatga
     5041 gcttctgaac cgggccggca tggatacgat ttccgcgggt gcgacggccg cgtttgccgt
     5101 cgagtgctac gaaaagggca ttctcaccag ggaagacacg ggcggcctgg agctgaaatg
     5161 gggaaacacg gaggcgattg tcgaactcat ccggaaaatg gccaaccgcg agggcatcgg
     5221 agaccttctg gccgacgggt cgaagattgc cgcccaaagg atcggcaaag gatcggaagc
     5281 ctgcaccgtc cacgccggcg gccaggagcc ggccatgcac gacggccgct gtgatccggg
     5341 tttcaatgtc cattacagcc tggagccgac gccgggccgt cacacgctgg gcgcccatct
     5401 gtattatgaa atgtttcagc tctggaagcg cgtgaaaggg attcccaaac ctttgccgct
     5461 tttcatcaag aacagcaagt tcagggtcga cggagaaaag gcgcggatgt ccgccgcgtg
     5521 cagcaagttc atgagtctgg ccaacggcgc cggcatgtgc atgttcggtc tcttcgtcgg
     5581 gatcaaaaga gttccaacct ttgactggct caacgcggcg acgggctgga accggacgcc
     5641 tgaagagtat atgaccgtcg gcgaacggat tcagaccctg aagcaggcct tcaatgtcaa
     5701 gcacggcgtt gagccgaaaa acaatttcat cagcagccgc gctctgggca cgattcccca
     5761 ggcagagggc gccaacaagg gaagatccgt ggacatcgag aagctggcga gcggatactg
     5821 ggagcagttc ggatgggacg cggccaccgg caagcccaaa gaggaaaccc tgcgcaaact
     5881 ggaaatacaa taagaggttg aatatggcct acacgatcac ggaaaaatgc aacggttgcg
     5941 gcgcctgcgc gcgcctgtgc ccggccggcg ccatcacggg cgaaaaaaag aagctccatg
     6001 cgatcgaagc ggttttatgc attgaatgcg gcgcgtgcgg ccggatctgt ccgcaggccg
     6061 ccgtgctgga cgcggcaggc gctccgtgcg cgatgatcaa acgctccgaa tggcccaagc
     6121 cggagattct gctgaaggcc tgcattgcct gcaccgtctg cgttgacgcc tgtccggtgg
     6181 gatgtctggc gatgtcggac ctctcccgcg ataaaggcgt ggacgcgttt ccgcatctga
     6241 gggactccaa ggcctgcatc ggctgcgggt tctgcagccg ggaatgcccc gcggacgcac
     6301 tggtgatgaa aaaaccggtc cccgccgccc aaacggttgc cgctgaatcc gcatgagtat
     6361 tgaaaccata ccgcggtatc caatcgacaa aatcatttcc ggaggccaga ccggcgcgga
     6421 ccgggcggcc ctggactttg ccatcgcccg caaccttcct tacggcggct ggcttccccg
     6481 ggggagaaag acggaggacg gcgccctgga tttgaagtat cgcctgcaag agatgccgac
     6541 gacggactat gccaaaagga cggagcagaa tgtcatcgac gccgacggca ccgtcatcgt
     6601 ctcccacgga tttccggttg cgggatcggc gctgacgctg gaattcaccc ggcggcacga
     6661 cagaccatgc ctccatatcg atctgaagga aatatccccg gagaaggcag cggaggtcct
     6721 ggtttcctgg cttggggaaa accgcattcg cgttctcaac gtggcgggac cgagggcggg
     6781 aagggatcca gccatctatc cggccgtgat ggatcttctg gagcgatcga tagaaaacgc
     6841 tgggccggtg gacgtcgttc tgtttgattt cggcggcgtg ctggcggagg aaggctggaa
     6901 agacggtttg caggcgattg cccacgccaa cggattgaaa gccgttgaac ttctcctgat
     6961 tgcggccgac acggtgtatg agacaggcta cattctgggc aaaggctccg aaatcagcta
     7021 ctgggatgcc atgcgccgca agaccggcat tcggggggat tatgcgcagt tgcgggatga
     7081 aattctgtcg cgtttcatcc ttcgggagaa tatgatcgat cttgtcaaaa gactgaaggc
     7141 ggacaatctt gtcgtcggta ttttgagcga tcagacggac atgctggacc aactgaacgc
     7201 ccggatgaat ttttttcagt ggtttgatta cgtcttcaac agctaccaca tgggcaaggg
     7261 caagcgggac gcaacgttgt ttgacgatat tgcccaacgg ctgaaaaccc tgccggggcg
     7321 gattctattc attgacgatg atccggggaa cgttgaacgg gcgaagcaga ggggctggaa
     7381 ggctatcctt tactccgatg aaaatgtttt tcagtcggaa ttggaccgtc tgctgagcgc
     7441 ttgaggaccg agaaaggctg ttcaggaaaa aggcgaaaga tacccgtccg gacgggacaa
     7501 agccaatatc gggtccctgt ttttgacgga ataaaaatcc attccctgcg ccgccaaacg
     7561 aaaaagcccc ccggggattt cccggagggc tttgtgtatc ttcaacagat c
//
LOCUS       DAOSIM010000089         7605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_8882_length_7605_cov_7.661325, whole genome shotgun sequence.
ACCESSION   DAOSIM010000089 DAOSIM010000000
VERSION     DAOSIM010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7605
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8882_length_7605_cov_7.661325"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /gene="cydB"
                     /locus_tag="PLR47_05205"
     CDS             <1..898
                     /gene="cydB"
                     /locus_tag="PLR47_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOG12374.1"
                     /translation="LGKTKEEKDTLIASIGPVWDGNEVWLITGGGALFAAFPHAYATA
                     FSGFYSAMMLVLLALIFRAVSMEFRANDPARAGLWEKTLIGGSALAALLFGVALGNVI
                     YGVPLDGRMEYAGSFFTLLRPVPLLFGITGLAAVLLQGASWAVLKTEGRVQERSFEAA
                     RTLTWINAVLAVIYFVVLIYAIPRVSGNALFYVAVAITFAGLIGLFFSLRKKNDAGPF
                     WESSFSLIGLFLAAGAAQFPNLITASNDPNFSLTIYNSSTSLYTLQVMAVIALAGMPI
                     VIGYTLFVYRLFKGKAKADGHY"
     gene            complement(922..2055)
                     /locus_tag="PLR47_05210"
     CDS             complement(922..2055)
                     /locus_tag="PLR47_05210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOG12375.1"
                     /translation="MKRDIAIDYLRSGVTLLVVAHHASLAYNTSSFYNADDYMKSSAP
                     VVDALRLLPLDFFVGWNDMFFMCLMFLISGLFIVPSLNRKGAGRFMTDRAKRLGVPFI
                     ISVFLLAPLAYFPSWLLSNTASQGGYLRGFFTNDGWASGPAWFIWVLLAYGAFVALAC
                     RFTPGLMKKLSWTAASPRNLVVVFLLASLAATLPLRLFVEPGHWAHIAGPLYFHAWRF
                     FLYFVWFLLGVALGGADPDRSLSRENLRPWPLWLFLGGITYLTHGTLEVNAAGLGITP
                     AWLMSVILTALYCLCCTFTGLAALGLARKFFHTARPVADHLTANAYGIYVFHYVFVIW
                     MQFFLLGQPLSPWIKFAAVFLTALAGSWLLTALLRKTPANQIL"
     gene            complement(2372..4909)
                     /locus_tag="PLR47_05215"
     CDS             complement(2372..4909)
                     /locus_tag="PLR47_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12376.1"
                     /translation="MKRDVGDMLTTLIRNQADREALETTAGAPGELPTLGVAPIRRGE
                     RHIAFDLSRIRVFRNLHTFVSLLAEEIIEQCAFGAADIMVRRKVDSRLTPELAAAGLD
                     WVTIYVRLDAAEGLPFHYRSFGDRLQMIFLTLQTERWGGALFPGYFGLQPSEASAPPA
                     LLFPFHLHDGGDPNGHYYLVEHSPSGHFLRITIEDAAHSRLQLKHIPHRVVDQPLRRS
                     FFTDIFSTAEKIHQGILHDVLNHRAEHVETSAHLPDFFAYLRQTGLPQLQQIRFTWPT
                     DDRQMMFLEKSDATALTESLDMLAREIQLLEDPAVLHLLEKGQTVEMLSGSFRIYFDV
                     SRWGACLNVSFDEIRERQDLADYLASMKALRAALALHPRALEGVRLFVIHHATAEALG
                     LLKAFADAQCDSLTAFFVQYAGIVPNDYLETLMSLPPQTFRFYGLQKMESRLRLAGSY
                     MLSRQFTPFSGLETMEQALIDEQPDFLDAMRMAAGHLFFKEADAARRANRLLLLVEDG
                     GYLAPIINRFCLEGKTLGEVFEHFHLPRPAEAFHMPLADWLAPVFPGSVEHTKNGYDY
                     LNEVAQAFGKLQYPALSIAVSDLKRGPEARACAVSILNAAENILHRLGLLLSRRTIVI
                     LGSAGAIGGFLRKELGRRLTKGCLFGVDVAAGNDAADGIQEAKTLDELGSGVLRNADM
                     FIGVTGLSLLQRGHLEEILLNGRQPAVFFISGSTKTVEFADAQHYLQSLRDTPNARIG
                     GRPVSADFKPLRDLQTGILQGYEIRLAFTDQPSCGKILYLLGEGMPLNFLYYGIPREI
                     VDEVMAQLFCVSCGLIRRLRAAATLPPALLAVDREIDANADLLPGKK"
     gene            complement(4927..5904)
                     /locus_tag="PLR47_05220"
     CDS             complement(4927..5904)
                     /locus_tag="PLR47_05220"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOG12377.1"
                     /translation="MGTMDTMAAAVFDGKLNYVEDYSVPPVKPGWAKIQVKIAGICKT
                     DMEILKGYMGFKGVLGHEFVGIVMECDDPSWIGKRVVGEINAACGHCDWCRRDLGRHC
                     PNRSTLGILNLDGCMAEYCTLPIVNLLALPEKLSDERAVLVEPLSAACEILEQMRPAG
                     NERAVILGDGRLGALCAWVLATVLPVVTVVGHHPEKLENLRWRNIRTVLHEKDVAPGA
                     DIVVEATGSGAGIVTAMALCRPRGTIVLKSTVAVSGDVNLAPIVINEQTVLGSRCGRF
                     ADGLKIMCEYPDMPLDRLITARYPLARVEEAFARATQSDALKVLLDIHP"
     gene            complement(5915..6943)
                     /locus_tag="PLR47_05225"
     CDS             complement(5915..6943)
                     /locus_tag="PLR47_05225"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOG12378.1"
                     /translation="MNPGAKKILLAGLAFAVVYGLLNSLSNIFLLPSAPIISMRPQIA
                     LPMVVGILVHPLAGFITGFAGNMFGDAVYGFGALKFWNWHIANGLMGWIPGLVGYGGI
                     RRIGTVREFGILQMSLVGASAAGVAAAVVLDVFFIHLLTFPYSFPSWILPAFLTDAVN
                     AFILVPVILLLARRIRLTLETRTILMITGLLLVSILATAGAITWAVLDDLVSHSAMVE
                     NFYIAAVVSVLLLVAGFIAAIVFVRKVTDPLADLAQAAQAVEQGNYDLPGLAGVSARA
                     DELGRLARIFEQMAGKVREREKTLQRQVDELKIIIDRKKQAQDVSEIVETEYFLALKE
                     KARKFRET"
     gene            complement(6940..>7605)
                     /locus_tag="PLR47_05230"
     CDS             complement(6940..>7605)
                     /locus_tag="PLR47_05230"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOG12379.1"
                     /translation="GRRVGVVDTDIQSPGIHVLFNLDPKDAPHTLNDFLWGKCDIGRA
                     VYDVSRILGAEDKEKLFLIPSSIRTGDIARVLHYGYDINLLKEGYQKAIEQFALDYLF
                     IDTHPGLNEETLMSIAVADTLLILLRPDQQDYQGTAVTVEVSRQLEVPRMFLIVNKVP
                     TAFPGDALAAKVTAAYNCDVAGVLPHADEMMALGSSGLFCLMHPNTALGRAMEDILRR
                     ITA"
BASE COUNT         1630 a         2154 c         2322 g         1499 t
ORIGIN      
        1 tctgggcaaa acaaaagaag aaaaggacac gctcattgct tccatcggcc cggtctggga
       61 cggcaacgaa gtctggctga ttaccggcgg cggcgcgctt tttgccgcgt ttccccatgc
      121 gtatgccacc gcgttttccg gtttttattc ggcgatgatg ctggtgctgc tggccttgat
      181 ttttcgcgcg gtttcgatgg aattccgggc caatgatccg gcgcgcgccg gtctctggga
      241 aaaaacattg attggcggaa gcgcgctggc ggctttgctg ttcggcgtgg cgctgggcaa
      301 tgtgatctac ggcgtgccgc tggacggcag aatggaatac gccggaagct ttttcacgct
      361 gcttcgtccc gtcccgctgc tgttcggcat caccggtctt gccgccgtgc tgctgcaggg
      421 cgcgtcctgg gcggtgctga aaacggaagg gcgggtgcag gagcgttcat ttgaagccgc
      481 gcggactttg acttggatca atgccgtcct ggcggtgatt tatttcgtgg ttctgattta
      541 cgccatcccg cgcgtgtcgg gaaatgcgtt gttttatgtc gcggttgcaa tcacctttgc
      601 gggactgatc ggattgtttt tttctcttcg caaaaaaaat gacgcaggcc cgttctggga
      661 atcgtctttt tcgcttatcg gactgtttct ggcggcgggc gcggcgcagt ttcccaacct
      721 gattaccgct tccaacgatc cgaacttcag cctgaccatt tacaacagct ccaccagcct
      781 ttacacgctg caggtcatgg ccgtcatcgc gcttgccggc atgccgattg tgatcggcta
      841 caccctcttt gtttatcgcc tgttcaaggg aaaggccaaa gccgacgggc attattagaa
      901 agcatttttc aggcgtcctg cctaaaggat ttgattggcc ggggtctttc tgagcagcgc
      961 ggtcaaaagc cagcttcccg ccagggccgt caggaaaacg gccgcaaact tgatccaggg
     1021 ggaaagcggc tgtcccagca ggaaaaactg catccagatc acgaagacat aatgaaaaac
     1081 atagatgccg taggcgttgg cggttagatg gtccgccacg gggcgggcgg tatggaaaaa
     1141 tttgcgggca agcccgaggg cggcaagccc cgtgaaggtg cagcacagac agtagagggc
     1201 ggtcaggatg acgctcatca gccatgcagg ggtgatgccg aggccggcgg cgttcacttc
     1261 cagcgttcca tgcgtcaggt aggtgatccc gcccagaaac agccacagcg gccacggcct
     1321 gagattttcc cgcgaaaggg agcggtccgg atctgcgccg cccagggcga cgcccagcag
     1381 aaaccacaca aagtacaaga agaatcgcca ggcgtgaaaa tacagggggc ctgcgatatg
     1441 cgcccagtgt ccgggttcga caaacaggcg cagcggaagc gtcgcggcca ggctggccag
     1501 gagaaagacc acgaccagat ttcgcggcga tgcggccgtc caggataact ttttcatgag
     1561 cccgggggtg aaccggcaag ccagtgcgac gaaggcgcca taggccagca ggacccagat
     1621 aaaccaggcc ggcccgcttg cccagccgtc gttcgtaaag aagccccgca gatagccgcc
     1681 ctggctcgcg gtgttgctta aaagccatga cggaaagtag gccagcgggg ccagcagaaa
     1741 cacggaaatg ataaacggaa cgccgagacg cttggcgcgg tcggtcataa agcggccggc
     1801 gcctttccgg ttcagggacg ggacaataaa gagtccggag atcaggaaca tgaggcacat
     1861 gaagaacatg tcgttccagc ccacgaaaaa gtccagcggc agcaagcgaa gcgcatcgac
     1921 gacgggggcc gaagatttca tgtaatcgtc tgcattatag aaactggagg tgttgtaggc
     1981 cagagaggca tggtgggcga ccaccagcag ggtgacgccg ctgcgcagat agtcgatggc
     2041 gatatctcgt ttcaaaaagt tcctccggac ggccaaggga tcggtttgtt cggcgaggat
     2101 cgtcaaaagc cggatcttcg ggcggcggcc gtcgcatcgt cagtattgcg gctgatccgt
     2161 tgggcgcttt tgacggaagc gcactatagg caatttattt cggcctgtca acccgatccg
     2221 gagcatccga gaaaaatgct ccgttcatca ttgcttactg cttgaacagt gaatggtcga
     2281 atagaattgg aaaagcaaca gcacaggaag caagcaaggc ccggataaat cttaaaaata
     2341 ttcttatcgc tttcggaaat ctcttccaat gttatttttt gccgggcagt aaatcggcgt
     2401 ttgcgtcgat ctcgcggtcc accgccagga gcgcgggcgg cagcgtagcc gcggcgcgca
     2461 ggcgccggat caggccgcag gacacgcaaa agagctgagc catgacttcg tccacgattt
     2521 cgcggggaat gccgtagtaa agaaagttca gcggcattcc ttcgcccaga aggtaaagga
     2581 tcttgccgca ggacggctga tccgtgaagg caagccggat ttcgtaaccc tgcagaatgc
     2641 cggtctgcaa atcccgcaag ggtttgaaat cggcgctgac cggccggccg ccgatgcgcg
     2701 cattgggggt atcgcgaagg gattgcagat aatgctgtgc gtcggcaaac tctaccgttt
     2761 tggtcgaacc gctgatgaaa aatacggccg gttgcctgcc gtttaggaga atttcctcca
     2821 gatggccgcg ctgcagaagc gataggccgg taacgccgat gaacatgtcg gcgtttctca
     2881 gtacgccgct tcccagctca tcgagggttt tggcttcctg gatgccgtct gcggcgtcgt
     2941 ttcccgcggc gacgtccacg ccgaagagac agcctttggt caggcggcgg ccgagttcct
     3001 tcctgagaaa gccgccgatg gcgccggccg agccgaggat gacgatcgtt cggcgggaaa
     3061 ggagcaggcc caggcggtgg agaatgtttt ccgcggcgtt gaggatcgag acggcgcagg
     3121 ccctggcttc cggacctctt tttaaatccg aaacagcgat gctgagcgcc ggatattgca
     3181 gttttccgaa cgcctgcgcg acctcgttta aataatcgta tccgtttttc gtatgttcga
     3241 cgctgcccgg aaacaccggg gccagccagt ccgccagcgg catatggaaa gcctccgcgg
     3301 ggcggggcag gtggaagtgt tcaaaaacct ctcccagcgt tttcccctcc agacaaaagc
     3361 ggttgatgat cggcgcgagg tagccgccgt cctccaccag cagcagcagc cggtttgccc
     3421 gccgggccgc atcggcctct ttgaaaaaaa ggtgtcccgc cgccatgcgc atggcgtcca
     3481 ggaaatccgg ctgttcgtcg atcagcgcct gttccatcgt ttccagcccg gaaaaaggag
     3541 tgaactgccg tgacagcatg tagctgccgg ccagtctgag gcgcgattcc attttctgaa
     3601 ggccgtaaaa tcgaaatgtc tgaggcggca ggctcatgag ggtttccaga taatcgttcg
     3661 gcacaatgcc ggcgtactgg acgaaaaaag cggtcagcga atcgcattgc gcgtcggcaa
     3721 aggccttcag cagaccgagc gcttccgcgg ttgcatgatg aatgacgaaa agccttacgc
     3781 cctcgagcgc ccggggatgg agcgccaaag ccgctcgaag cgccttcatc gacgcgaggt
     3841 aatccgccag atcctgcctt tcgcggattt cgtcgaagct cacgttgagg caggcgcccc
     3901 agcgggaaac gtcgaaatat atgcgaaacg atccggaaag catctcgacg gtctgcccct
     3961 tttccagcag atgcaaaacg gccggatcct ccagcagttg aatctccctg gccagcatgt
     4021 cgagcgattc ggtcagggcg gtcgcgtcgc ttttttccag aaacatcatc tgccggtcgt
     4081 ctgtgggcca ggtaaagcga atctgctgca gttggggcag gccggtttgc cgcagatagg
     4141 cgaaaaagtc cggcaggtgc gccgacgttt caacatgctc ggcgcgatgg ttgaggacgt
     4201 cgtgcagaat gccctggtgg attttttcag ccgttgaaaa gatgtcggtg aaaaaagagc
     4261 gccgaagcgg ctgatcgacc acgcgatgag gaatgtgttt gagctgaagg cgcgaatggg
     4321 ccgcgtcctc aatcgtgatg cgcagaaaat ggcccgatgg gctgtgctcc accagatagt
     4381 aatggccgtt tggatccccg ccgtcgtgaa gatgaaacgg aaacagcaga gcgggcggcg
     4441 ccgacgcctc ggacggttgc agaccgaaat agccgggaaa gagcgcgccg ccccaccgtt
     4501 cggtttggag cgtcagaaaa atcatctgca gtcgatcgcc gaaactccgg taatggaagg
     4561 gcaatccttc cgccgcatcg agccttacat aaattgttac ccaatccaga ccggcggcag
     4621 ccagctccgg cgtcaggcgg gaatccactt tgcggcgcac catgatgtcg gcggcgccga
     4681 aagcgcactg ctcgatgatc tcctccgcca ggagcgagac aaatgtgtgc agattgcgga
     4741 aaacgcggat gcgggaaaga tcgaaggcga tgtgacgctc gcctcgccgg atcggcgcaa
     4801 cgccgagcgt cggaagttcc ccgggcgcgc cggctgttgt ttccagagct tcccggtcgg
     4861 cctgattccg aatcagggtt gtgagcatgt cgccaacgtc gcgcttcatc tttttttcgc
     4921 cttcgtttaa gggtgaatgt ccaggagaac cttcagcgcg tcgctctgtg tcgcccgggc
     4981 aaaggcctcc tccacgcgcg ccagcggata gcgggcggta atcagccggt ccagcggcat
     5041 gtccggatat tcgcacatga ttttgagtcc gtcggcaaac cgtccgcagc gcgatcccag
     5101 aaccgtctgc tcattgatga cgatcggcgc cagattgacg tcgccggaaa cggcaacggt
     5161 ggatttcagc acaatagttc cgcgcggccg gcacaatgcc atggctgtga cgattccggc
     5221 gcccgacccc gtggcctcca cgacgatatc cgcgccgggc gcaacatcct tttcatggag
     5281 gacggtccga atgtttcgcc accgaagatt ctccagtttt tccgggtgat gccccaccac
     5341 cgtgaccacc ggtaaaacgg tggcgagcac ccaggcgcaa agagcgccga ggcgaccgtc
     5401 gccgagaatc accgcccgtt cgttgcccgc gggccgcatt tgctccagga tttcgcaggc
     5461 cgccgacagg ggctccacga gaaccgctcg ttcgtcggaa agtttttccg gaagcgccag
     5521 caggttgacg atgggcaggg tgcaatattc ggccatgcac ccgtcgaggt tgagaatgcc
     5581 gagggtcgaa cggttcgggc agtgacgccc cagatcgcgc cggcaccagt cgcaatgccc
     5641 gcaggcggcg ttgatttcgc cgaccactct cttgccgatc caggaggggt cgtcgcattc
     5701 catgacgatg ccgacaaact cgtgccccag cacgcctttg aaccccatat agcctttcaa
     5761 aatttccatg tcggttttgc agatgccggc gatctttact tgtattttag cccagccggg
     5821 cttgacgggc ggcacggaat aatcctcgac atagttgagt ttgccgtcga aaaccgccgc
     5881 tgccatagtg tccatcgtcc ccatcgtctt ctccttatgt ttccctgaat ttgcgggctt
     5941 tttctttcag cgcaaggaaa tattccgttt caacaatttc ggaaacatcc tgcgcctgct
     6001 ttttccggtc gatgataatt ttcagttcat cgacctgtcg ttggagcgtt ttttcccttt
     6061 cccggacttt tccggccatc tgctcgaaaa tgcgggccag ccggcccagt tcgtctgcgc
     6121 gcgcggacac gcccgccagt ccgggcagat cgtagttgcc ctgttccacg gcttgcgcgg
     6181 cttgggcgag atccgccagc ggatcggtca cctttcggac aaaaacgatc gcggcgataa
     6241 agcccgcgac taaaagcagg acggaaacga cggcggcgat gtaaaagttt tccaccatcg
     6301 cgctgtggga aaccagatcg tcgaggaccg cccaggtgat ggcgccggcg gtggccagaa
     6361 tgctcacgag cagaaggccg gtgatcatca ggatggtccg cgtctccagc gtcaggcgaa
     6421 tgcggcgggc cagaaggaga atgaccggca ccagaatgaa agcgttgacc gcgtcggtga
     6481 gaaacgcagg cagaatccag gacggaaagg aatagggaaa ggtgagcaga tgaataaaga
     6541 aaacgtcgag cacgaccgcc gccgcaacgc ccgcggcgct ggccccgacc aggctcatct
     6601 gcaggatgcc gaattcccgc accgtgccga tgcggcggat tcccccgtag ccgacaagcc
     6661 ccggaatcca tcccataagg ccgttggcga tatgccagtt ccagaattta agagcgccga
     6721 atccgtaaac ggcgtcgccg aacatgttgc ccgcaaatcc agtgatgaag ccggccagtg
     6781 gatggaccag aatgcccacg accatcggaa gagcgatctg cggccgcatg gagatgatcg
     6841 gcgcggaggg cagcaggaaa atgttggaaa ggctgttcaa gagcccatag acgacggcaa
     6901 aggccaaacc ggccaggagg attttttttg cgcccggatt catgccgtaa ttctccgaag
     6961 gatatcttcc atcgcccggc caagcgccgt attcggatgc atcagacaaa acaggccgga
     7021 gctgcccagc gccatcattt cgtcggcgtg cggcaggacg ccggccacgt cgcagttgta
     7081 tgccgccgtc actttggcgg ccagcgcgtc gcccgggaaa gccgtgggga ctttgttgac
     7141 gatcagaaac attcggggaa cttcaagctg tctcgacacc tcgacggtca cggctgttcc
     7201 ctgatagtcc tgctgatcgg ggcgcagcag aatgaggagg gtgtcggcga cggcgatcga
     7261 catcagcgtc tcctcgttca agcccggatg ggtgtcgatg aaaagataat cgagcgcaaa
     7321 ttgttcaatc gctttctgat agccttcctt gagcagattg atgtcgtagc cgtaatgcag
     7381 gacccgggcg atgtcacccg tgcggatgct cgacggaatg agaaaaagct tttctttatc
     7441 ctccgctccg agaatccggg acacgtcgta aaccgcgcgt ccgatgtcgc atttgcccca
     7501 gagaaaatcg tttaaggtat ggggcgcgtc tttgggatcg aggttgaaca gcacgtgaat
     7561 gccgggagac tggatgtccg tatccacgac gccgacgcgc cggcc
//
LOCUS       DAOSIM010000090         7599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_8898_length_7599_cov_7.308987, whole genome shotgun sequence.
ACCESSION   DAOSIM010000090 DAOSIM010000000
VERSION     DAOSIM010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8898_length_7599_cov_7.308987"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..657)
                     /locus_tag="PLR47_05235"
     CDS             complement(106..657)
                     /locus_tag="PLR47_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOG12380.1"
                     /translation="MKPTEILKEEHRLVLLMLKIMEAVCKKLEQNQSVKTEDLDAIID
                     FIRGFADGCHHAKEEKLLFPALEKTGMPQTDGPIAVMLREHALGRGFARSMSDAIGLM
                     KNGDATAAKTFVENARGYIQLLWNHIDKEDHVLFMMAEQRLSPAAQDELVEGFDRVER
                     EETGEGVHEKYHALLHRMRDDYL"
     gene            886..1254
                     /locus_tag="PLR47_05240"
     CDS             886..1254
                     /locus_tag="PLR47_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12381.1"
                     /translation="MSEETHKKEHKSHDDQHLDKLTVKELRELAEQFPHEKAVHEMKK
                     EELVAFIKEAKGIKDEPHAHKHKPKGKIRMTKPEVKAKIRELKFLHGRALEDSDREKA
                     LLLRRQISRLKKISRRVAEA"
     gene            complement(1271..2176)
                     /locus_tag="PLR47_05245"
     CDS             complement(1271..2176)
                     /locus_tag="PLR47_05245"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF010621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOG12382.1"
                     /translation="MWTSITGFLRGALGLAFLGINTLFWAVPVYITAVFKLILPFRAA
                     RNFFDVILNFLSYTWIYCINWFIGAIKTVEYDVTGLEKLKRNEWYIVVSNHQTMIDII
                     VLQKIFHGRIPFLKYFMKQELIWVPILGLAWWALDYPFMKRYSSELLKRKPHLRGKDV
                     EATRRACAKFKDRPASIMNFVEGTRFSRAKQAAQKSPYRHLLKPRSGGVSYVLSMMGE
                     NMRNLIDVTIVYPNGVEDAWRFFCSRISRILVRIEVLPIDPDLRGKNPDDQEYRNRFN
                     RWLNDLWKKKDCTIEQTLARAFSRP"
     gene            complement(2363..2626)
                     /locus_tag="PLR47_05250"
     CDS             complement(2363..2626)
                     /locus_tag="PLR47_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12383.1"
                     /translation="MSTQLAVPLSQVALLLGISTLVLFFGRVKLALLINYCFTFYWGF
                     FLNPSFRSELGELVLNTYTYVYIGVGLLIVVLALIGFLSSNER"
     gene            complement(2678..3016)
                     /locus_tag="PLR47_05255"
     CDS             complement(2678..3016)
                     /locus_tag="PLR47_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12384.1"
                     /translation="MKRKWTALLAAMVICISAVPACAEVTQSDLVVLDVALIRPVGIV
                     SVAVGTALFIVSLPVSIPSGSVGAVAKSLVKDSFEYTFIRPVGNFDYELGTWGKEAPP
                     ISENSRSRIK"
     gene            3210..4043
                     /locus_tag="PLR47_05260"
     CDS             3210..4043
                     /locus_tag="PLR47_05260"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG12385.1"
                     /translation="MTGIPEKIIDFHVHLFPDRLFDAIWEAFLKQYNWHIIHRCYYRE
                     CIERLRRAGVSVIVYSNYAHRAGIAEDLNRWNEKVLQEYEDLYCFAAYHPGDDHALDM
                     ARRLVENPKILGFKLQLLVQNFYPYDERLFDLYELVIRKNKRILFHTGTGPVGNPYVG
                     LDPFMKVLDRFPDLPATIAHLGAHEYAGFMELADRHPALYLDTAFCFLPAPYEGYNLG
                     PGYLEKYKSRILYGSDYPNLITPWEAEIENLLKMNLSSEFYDRIFHDNAAALIRSLTK
                     A"
     gene            4099..4494
                     /locus_tag="PLR47_05265"
     CDS             4099..4494
                     /locus_tag="PLR47_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOG12386.1"
                     /translation="MNHVHHVHLFASDIEKSLAFYQNAFGGKIILDEELAGARNVFMR
                     VGTGRIHFYDQAPSFSGRGSIHHFGIQTDDIDGVVEKLKAMGVPFKKSVTDIGVWKYV
                     MVPAPDNVLIELFQVNRDALPEAYRSYFD"
     gene            complement(4550..5974)
                     /locus_tag="PLR47_05270"
     CDS             complement(4550..5974)
                     /locus_tag="PLR47_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOG12387.1"
                     /translation="MNLDALFYPKSVAVIGASDNLGGGKLPYFQILQANGFAGALYPV
                     NPRRAEVGGVKTCASIEDLPEPVDFAIVAAPVEQSVDIVKAAVRKQIKFIHFFTSGFG
                     ETGNLQLEQEVIREARRGHLRIVGPNCIGVQCFESKVSFRLTHLQEKPGSIAFLGQSG
                     GITSSFVAMATTRGIGLNKVVSYGNQIDLRLEDYLEYLADDDKIRLIACYVEDIKDVN
                     KFLKTLRRTTARKPVIILKGGKTELGSKAAASHTGAMASNYTIWAAAVRQHGGLLVDD
                     FEELMNLAMLGTAQRFPRGRRVGFMGAGGGISVLFSDLAVTSGVLLPELSPGTQRMIS
                     EKIMGVNTSTTNPVDLGAFGFDFNVMLHTMKALNEDVNIDVIVPYFSVEYIMHAEVFL
                     KVTNSADSIMAMAQQIKKPVIPVLSCFLENDLEAERVRISTFNALRKAGFPVYSTIQE
                     ALYVIETYFEWSEKRVRGSRKTAE"
     gene            6164..7096
                     /locus_tag="PLR47_05275"
     CDS             6164..7096
                     /locus_tag="PLR47_05275"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG12388.1"
                     /translation="MAFARPEILRPPSEHASYYLPLTSGCSNNSCAFCAFSFTSLGIR
                     DLDDVKREIDAMSLYVNSRMWMAGQPDIVYAILRQWDGKRVFLQDGDALVYPYPKLME
                     ALRYLNGKFPALERIASYATPQDILRRSVQELRDLKEQKLGILYMGVESGDDEVLKKI
                     RKNVTHDQMVEAAKKVKASGILLSVTVILGLGGVAGSEKHALESARILTEMDPDYAGA
                     LTLTLIPETELYKEWEQGRFEMITPFDSLRELKTMVEHSTFSNCFFSSMHASNYFSIR
                     GTMPKEKGRVLRQLQALLSRKDPGMLRPEFMRGL"
     gene            7281..>7599
                     /locus_tag="PLR47_05280"
     CDS             7281..>7599
                     /locus_tag="PLR47_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12389.1"
                     /translation="MRTDLNFSYKFNEKYRAVSYVFVQANDEMSNYDYTEWGAGLQYQ
                     TPAAWLSFLAYYQQGYSKDDDGHWALEQKPSINLNLSKIFFNFKIFNQIRYEYRITPD
                     WNDA"
BASE COUNT         1741 a         2020 c         2081 g         1757 t
ORIGIN      
        1 agtgtcggca gttcaattct gccctgaccc accagtaaaa tcaaggggtt agccaataaa
       61 tggcggaccc cttttttctt ggaatagaca ttccaacgct tttttctaca gataatcgtc
      121 gcgcatgcgg tgcagcaggg cgtggtactt ttcgtgaact ccttcgcccg tctcttcgcg
      181 ctccacgcgg tcgaagcctt ccacaagttc atcctgcgcg gcgggactca ggcgctgttc
      241 ggccatcata aacagtacat ggtcttcctt gtcgatatga ttccataaca gttggatata
      301 gccgcgggcg ttttccacaa aagttttggc ggccgtcgcg tcgccgtttt tcatgaggcc
      361 gatggcgtcg ctcatgcttc tggcaaaacc gcggcccagg gcgtgttcgc gaagcatcac
      421 ggcaatgggt ccgtcggttt ggggcatgcc ggttttttcc agcgcgggaa aaagcagttt
      481 ttcttccttg gcgtggtggc aaccgtcggc aaaaccccgg atgaaatcga tgatggcgtc
      541 aaggtcttcc gttttcacgg actggttttg ctccagcttt ttgcagaccg cctccatgat
      601 tttcagcatc aaaaggacta gccggtgttc ttctttcaaa atttcggtcg gtttcatgag
      661 ttcctcctcc ttgaaaaaaa atctgaagcc aacagttttg cgcgcggccc ttcggcattt
      721 tgtttgcgca gagaataagg caaatgcaat gtcgtgtcaa tcggaaggaa tcggctgcgc
      781 gggttgaacc tcaagcgcgg ttgtggtata ggagatccaa accggtttcc ctttttcgca
      841 ttgcacgttg atgttcctga aatcaaacaa ggaggtctat gagcgatgtc cgaagaaact
      901 cacaaaaaag agcacaaatc ccatgatgat cagcacctgg acaaactgac cgtgaaggaa
      961 ctgcgggaat tggccgagca gttcccgcat gaaaaggccg ttcacgaaat gaagaaggaa
     1021 gagctggtgg cctttatcaa agaggccaag ggcatcaagg acgagccgca cgcgcacaaa
     1081 cacaagccca agggcaaaat caggatgacc aaaccggaag tcaaggcgaa aatccgcgag
     1141 ttgaaatttt tgcacggccg ggcgctggag gacagcgacc gggagaaagc cttgctgctg
     1201 cgccgccaga tcagccggct taaaaaaata tcccggcggg ttgccgaggc gtaagcccga
     1261 cggccgggcg tcaggggcga gaaaaagcgc gcgccagcgt ctgctcgatg gtgcagtctt
     1321 tctttttcca caggtcgttc agccagcggt tgaaccggtt tctgtattcc tgatcgtcgg
     1381 gattcttccc gcgaaggtcg ggatcgatcg gcagcacttc gatccggacg aggattcttg
     1441 atattctgct gcagaaaaac cgccaggcat cttccacgcc gttggggtaa acgatggtga
     1501 cgtcgatcag gtttcgcatg ttttcgccca tcatggaaag aacatacgat acgccgcccg
     1561 accgcggctt gagaaggtgc cggtagggcg atttctgcgc cgcttgtttc gcgcgggaaa
     1621 agcgggttcc ttcaacaaag ttcatgatgg atgcgggccg gtccttgaac ttcgcgcagg
     1681 ctctccgggt tgcctcgaca tctttgcccc gaagatgcgg ttttcttttc aggagttccg
     1741 aagagtaccg cttcataaaa ggataatcca gcgcccacca ggccagcccc aaaataggca
     1801 cccagatcag ctcctgcttc atgaaatatt tcaggaaggg gatgcggccg tgaaatattt
     1861 tttgaagaac aatgatgtcg atcatggtct ggtggttgga aacgacgatg taccattcgt
     1921 tccgcttcag tttttcaagc cccgtgacgt catattccac ggttttgatg gccccgataa
     1981 accagttgat gcagtagatc caggtatagg atagaaaatt gagaatgacg tcaaagaagt
     2041 tcctggccgc gcgaaagggc aggatcagct tgaaaacggc cgtgatgtaa accggaacgg
     2101 cccagaagag ggtattgatg cccagaaagg cgagaccaag ggcaccgcgc aaaaatccgg
     2161 tgatggatgt ccacatcgct ttcgctcctc gtttttcctt ggtgtgacct tccgtccatg
     2221 tataggtcat ttgcggggaa aaaatcaaat gcaaagcggc ttttttttcg aaaacacccc
     2281 tggtctgcga cacccgaacc cggtccgggg caggcgcaaa gtcgcgcttc gcttgcacac
     2341 aggaatgaaa taaaaggatg gctcagcgtt cgttgctgga aagaaatccg atcagggcca
     2401 gaacgacgat gagcaggccg accccaatat agacgtaggt gtaggtgttg agaaccagtt
     2461 ctcccagttc ggagcggaag ctgggattca gaaaaaaccc ccagtaaaaa gtgaagcagt
     2521 agttgatgag cagggccagc ttcacccgtc caaaaaacag gaccagggtg ctgatcccca
     2581 gaagcagggc cacctgggaa aggggcacgg ccagttgtgt gctcatgagg atttccatgg
     2641 cttctttata ccggagcagt tatttcttgt ccagcgccta ttttatccgg ctgcgggaat
     2701 tttctgagat gggcggggcc tctttgcccc atgtgcccag ctcatagtcg aaattcccga
     2761 cgggcctgat gaacgtgtat tcgaagctgt ccttcacgag gcttttcgcc acggcgccga
     2821 cgctgccgct gggaatggac acgggaagcg acacaatgaa cagtgccgtg ccgactgcga
     2881 cggagacgat gcccacaggc cggatcagcg ccacgtcgag gaccacaagg tcggactgcg
     2941 tcacttcggc gcacgcgggc acggcgctga tgcagatgac catcgcggca agcagggcgg
     3001 tccattttct tttcatcaca ttcccccgga aaagcggttt aagggttttg ccgttttctt
     3061 tagcccacat tttccgccgg gtcaagaaat ccccgccgcc cgtacgcgcc ggggaagggc
     3121 atctttgcac ccgccgacct ttccgttgat tatgaattcc ggttgtgcta accaaacagg
     3181 cgcatacaga cgcgcgggcg gtggcgacca tgacgggaat tccagagaaa ataattgatt
     3241 ttcacgttca tctgtttccg gacaggcttt tcgatgccat ctgggaagct tttctgaagc
     3301 agtacaactg gcacattatt catcgctgtt attaccggga gtgcatcgag cgccttcggc
     3361 gggcgggcgt gagcgtgatc gtgtattcca attacgcgca ccgcgccgga atcgccgagg
     3421 atctgaaccg gtggaatgag aaagtccttc aggagtacga ggatttgtat tgctttgccg
     3481 cctatcatcc gggcgacgac cacgctctgg acatggcccg ccgccttgtg gaaaacccga
     3541 aaatcctcgg attcaagctt cagcttctgg tccagaattt ttatccgtac gacgagaggc
     3601 tctttgacct gtacgagctg gtgatccgga agaacaagcg cattctcttc cataccggga
     3661 ccggccccgt gggaaatccc tatgtcggcc ttgacccgtt tatgaaagtt ctggaccgtt
     3721 ttcctgacct tcccgccacc atcgcccatc tgggcgctca tgaatacgcg ggtttcatgg
     3781 agcttgcgga ccggcatccg gctttgtatc tcgacaccgc cttctgtttt ttgcccgcgc
     3841 cgtacgaagg gtacaacctc ggacccgggt atcttgaaaa atacaaatcc agaattttat
     3901 acgggtcgga ttatcccaac ctgatcacgc cgtgggaagc ggagattgaa aatcttttga
     3961 agatgaatct ttcttcggaa ttttacgatc ggatctttca cgacaacgca gccgcgctga
     4021 tccgttccct gacgaaagca taggcggcgg catccccccg tttgcctgcg cgacgcccgt
     4081 aaagaaagga gcaagtccat gaaccatgtc caccatgttc atttgtttgc gtcggatatc
     4141 gaaaaaagcc tggcctttta tcaaaacgcc tttggcggga aaatcattct cgacgaggag
     4201 ttggcgggag cgaggaacgt gttcatgagg gtcggtaccg gacggattca tttctacgat
     4261 caggcgcctt ccttttccgg ccggggaagc attcaccatt tcggcatcca gaccgacgac
     4321 atcgacggcg tggtcgaaaa gctcaaggcc atgggcgtgc cgttcaaaaa aagcgtcacg
     4381 gacatcggcg tctggaaata tgtcatggtg cccgcgcccg acaatgtgct cattgaattg
     4441 ttccaggtaa accgcgatgc ccttccggaa gcctacagaa gctattttga ctgaaccgtc
     4501 cgggacggcg gatattccgg ggccctcgtt ttcttaccgt atcttttcgc tattcggcgg
     4561 ttttcctgct cccccgcaca cgcttttccg accattcgaa ataggtttcg atgacgtaaa
     4621 gagcctcctg gatggtggaa taaacgggga atcccgcctt gcgcagcgcg ttgaaggtgg
     4681 aaattctgac gcgttccgcc tccaggtcat tttccagaaa gcacgacagg acggggatca
     4741 ccggcttttt gatctgttgg gccatcgcca tgatgctgtc cgcgctgttg gtcaccttga
     4801 gaaacacttc cgcgtgcatg atgtattcaa cggaaaaata gggaacgatc acatcgatgt
     4861 tgacgtcttc gttcagggct ttcatggtgt gcagcatcac gttgaagtca aagccgaagg
     4921 ctccgagatc gacgggattg gtcgtcgagg tgttgacccc catgattttt tcactgatca
     4981 ttcgctgggt gccggggctc agttcgggaa gcaaaacccc cgaggtgacg gccagatcgg
     5041 aaaagagcac ggagatgccg ccgccggcgc ccatgaatcc cacgcgcctt ccgcgcggga
     5101 atcgctgcgc ggtgccgagc atcgccagat tcatcagctc ttcgaaatca tccaccagca
     5161 gcccgccgtg ctgccgcacg gccgcggccc aaatcgtgta attgctggcc atggcgcccg
     5221 tgtggctggc cgccgccttg gagcccagct ccgtttttcc ccctttaaga ataatcaccg
     5281 gctttctggc cgtcgtgcgc ctcagcgttt tcagaaattt gttcacatcc ttgatgtctt
     5341 ccacgtagca agcgatcaga cggatcttgt cgtcatccgc cagatactcc agataatcct
     5401 ccagtctcag atcgatctga tttccgtagg agaccacttt attgagcccg atgccccggg
     5461 tggttgccat ggccacaaag ctggaggtga ttccgccgga ctgccccaaa aaggcgatcg
     5521 aaccgggctt ctcctgcaga tgggtcagtc ggaaagaaac tttcgactcg aagcattgaa
     5581 cgccgatgca gttggggccg acgatccgga gatgtcccct gcgggcttcc cggatgactt
     5641 cctgctccag ttgcagattg cccgtttctc cgaaaccgga ggtgaagaaa tggataaact
     5701 tgatctgctt gcggacggcc gctttgacga tgtctacgga ttgctcgacg ggtgcggcga
     5761 cgatggcaaa atcgacgggt tccggaagat cttcgatgga cgcgcaggtc ttgacgccgc
     5821 ccacttcggc ccgtcgggga ttcacgggat aaagggcgcc ggcaaaaccg ttggcttgca
     5881 ggatctgaaa atagggcagt tttcctccgc ccagattgtc ggatgcgccg ataacggcga
     5941 cgcttttggg ataaaacaac gcatcgagat tcatgaaatt cctccttgat ctatatccgg
     6001 ccgtggcgaa ggatttgtat tcgcaaaaag ccggtttcaa aacggcgcta tcttacaact
     6061 ttgaagcaac gatgcaagaa aaaaacgaac ctgtcaggac ggtcttgaca aacgggcggc
     6121 tcggtctgta tggtatcaca atatttatgg aagggagccc attatggctt ttgcaagacc
     6181 ggaaatcctt cgaccgccca gcgagcacgc cagttattat ctgcccctga cgtccggatg
     6241 ttccaacaat tcctgtgcgt tttgcgcttt ttcctttacc agcctcggca ttcgcgacct
     6301 cgacgacgta aaacgcgaaa tcgacgccat gtctctgtat gtgaacagcc gcatgtggat
     6361 ggcgggccag ccggacatcg tctatgcaat tctccgccag tgggacggaa agcgggtgtt
     6421 tctccaggac ggcgacgctc ttgtgtatcc ctacccgaag ctcatggagg cgctgcggta
     6481 tctcaacggc aaatttcccg ctttggagcg cattgccagc tacgccactc cgcaggacat
     6541 tttacggcga agcgttcagg aacttcggga cctgaaggaa cagaagctcg gcattcttta
     6601 catgggggtg gaaagcggcg acgacgaggt cttgaagaag attcgaaaaa acgtcaccca
     6661 cgatcagatg gtggaagccg caaaaaaagt gaaggcatcc ggcattctcc tgtccgtcac
     6721 ggtgatcctc gggctgggcg gcgtcgcggg aagcgagaag cacgcgctgg agtcggcgag
     6781 aattctgacg gaaatggacc cggattacgc cggcgcgctg acgctcacgc tgattcccga
     6841 aacggaactt tacaaggaat gggagcaggg ccgctttgaa atgatcaccc cttttgattc
     6901 gctgcgggaa ttgaaaacga tggtggagca ttcaaccttt tccaactgtt ttttcagctc
     6961 catgcacgct tccaattatt tttccatccg cggcacaatg ccgaaggaaa aaggcagggt
     7021 gctccggcag cttcaggcgc tcttgtcccg gaaagacccg ggcatgcttc gcccggaatt
     7081 tatgcggggg ctgtaaaaag attgcccagt ggaacgatga tgaaattttg ctgaagacat
     7141 ccagtccgtg cgccaagaac agtcaaggga cagggttgat gatgcgaaga atatgttttt
     7201 tgtgcttcga tattgttctt gctgcatcct tgctcatgtt gatgtctgct tcgcttcttt
     7261 atgcgggcca tgacacatct ttgcgtacgg atttaaattt ttcctacaaa ttcaatgaaa
     7321 aataccgcgc cgtctcctat gtcttcgtcc aggccaacga tgagatgtcc aattacgact
     7381 atacggaatg gggcgcaggt ctccagtatc agacgccggc tgcctggctt tcctttctgg
     7441 cctattatca gcagggttat tccaaggatg acgacgggca ctgggctctg gagcaaaaac
     7501 caagcatcaa tctgaacctg tccaaaattt tttttaattt caagatattc aatcagattc
     7561 gctatgaata cagaattacg ccggactgga atgacgcca
//
LOCUS       DAOSIM010000091         7581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_8923_length_7581_cov_9.454558, whole genome shotgun sequence.
ACCESSION   DAOSIM010000091 DAOSIM010000000
VERSION     DAOSIM010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7581
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8923_length_7581_cov_9.454558"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PLR47_05285"
     CDS             complement(<1..511)
                     /locus_tag="PLR47_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOG12390.1"
                     /translation="MSLAKIPKPEELMKIGYKPPATAWMKTPVDFRPGTWSYAAAAKN
                     LAALDMPNPRAWQPSDSDWKLPENWRQIILDGMKERLEKYRSFRLFMDICVRCGACAD
                     KCHFYIGSGDPKNMPVLRAELIRSVYRRYFTKAGMCFGTLAGARDLTVDVLKEWFYYF
                     YQCTECRRCS"
     gene            complement(514..1497)
                     /gene="dsrM"
                     /locus_tag="PLR47_05290"
     CDS             complement(514..1497)
                     /gene="dsrM"
                     /locus_tag="PLR47_05290"
                     /inference="COORDINATES: protein motif:HMM:NF038037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrM"
                     /protein_id="HOG12391.1"
                     /translation="MNILLSFIAILAIALAAAAAGRADAPGWFRILPYAAFGLFLAGF
                     VYRVIRWAKAPVPFRIPATCGQGKSLPWIKSNPLDNPAGPLGAIGRMAAEIFLFRSLF
                     RNSQTEIVDGRPVYGSAKWLWFFGLMFHGSLLVVVLRHLRFFTEPVLPAVTGLSALDG
                     FLEIGVPALYLSDATLLAGLAFLFLRRAVLPQVRYISLFADYFALLLIAAVAATGLLM
                     RHFFPVDLNAVKTMAMGWTTFAPVAPKDGSVILYMHLCFVFILIAWLPFSKLMHLGGI
                     FMSPTRNMINNNRSLRHINPWNTPVKVHTYKEYEDEFRDKMKAAGLPVEKE"
     gene            complement(1525..3135)
                     /locus_tag="PLR47_05295"
     CDS             complement(1525..3135)
                     /locus_tag="PLR47_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOG12392.1"
                     /translation="MKDRITPADISRGSAGLCRIEPGDLAPLPPPYEDFNEAVLKPLA
                     AEKIKGLETSPDDTLAIGIAEPANKQEEEELVRRFLAGLKKLFEKENNWTFLQPLLLS
                     MEHCARCQTCSEACPVFEMSGRQEIYRPIFRSEVLRRIYKKYLKPGGRITAKWTGDID
                     LNWKAIARLAELSYRCSLCRRCASVCPIGVDNGLIAHEIRKLFSQEMGIAPKELHEKG
                     TVLHLRAGSSTGMNPLAAKDSVEFIQEEMEEITGCKVEIPWDVQGADILLLHNAGEML
                     AWPENVGAFAILFQAAGLSWTLSSEAAGYDAVNYGLWYDDVQFARVAVKHADIARRLK
                     VKKIVLGECGHAHKALTVVADRLLTGDLNIPRESALVTLEDIVMSGKLTFNPQKNDFP
                     VTLHDPCNVVRSMGIVAPQRRILGKIAPRFREMTPHGVNNYCCGGGSGFAIMSGYNFA
                     EWRNLVSSRKKFLQILQAFESEPAETPKYVCAPCSNCKGAIRDMIAYYHAEERSHLYY
                     GGLVELMVNALEGMKKPIIHFGEEPSWK"
     gene            complement(3132..4067)
                     /locus_tag="PLR47_05300"
     CDS             complement(3132..4067)
                     /locus_tag="PLR47_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HOG12393.1"
                     /translation="MGFYIFSYLCTVIFLVAVACRVYRQLTLPVHVRWEIYPVQHEPA
                     NKAAYGGSYMEELNWWKKKRERSPVNELLYMAPEIGLMRGLWKENRNLWLFSFPFHAG
                     MYLMLVVFALLLLSAVLFLLGLAEDSAVSSIINVLIAAAGWIGILAGTAGSLGMLYKR
                     LADRNLRAYATPADYFNLVFILLFFVCALLSALAVDPCFPAARLYVLGLLTAGAAAPA
                     YVPGQSLFGALTILLGSLLAAYIPLTHLSHMFMKFFLYHKVKWDDSPNTPGSPIASAI
                     RRNLQYRPTWKAAHVDADGKKTWQDIASAGPKEMK"
     gene            complement(4210..4530)
                     /locus_tag="PLR47_05305"
     CDS             complement(4210..4530)
                     /locus_tag="PLR47_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HOG12394.1"
                     /translation="MPQIELAGKIYEVDEDGFLQELDKWNEEFAQAYAHTEDIEGPLT
                     EEHWKIINYLRDYFAKFGIAPMVRKVCKDNGIDMKKLYELFPTGPAKGACKLAGLSKP
                     TGCV"
     gene            complement(4533..5966)
                     /locus_tag="PLR47_05310"
     CDS             complement(4533..5966)
                     /locus_tag="PLR47_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HOG12395.1"
                     /translation="MNQQRPRFIMAALRGGAGKTTLSLAVTVALRRQGLMVTPFKKGP
                     DYIDAAWLGRAASGPCYNLDTYLMGARQVVRSFSRHAAEGTFSLIEGNRGLFDGVNAA
                     GEFSTAELAKLLYAPVILIVDCDKVTRTAAAMVLGCLHLDPAVDIRGVILSRVNGKRH
                     ASVIRRAVEESTGLPVYGTVPRLPDLPFVQRHLGLIPPAEHADVQQALDTAGAIARDY
                     LDIPSLVKIARSAPPWKVGAFPKAAPSVRDMDPPVTIGVISDAAFQFYYAENIEALAD
                     RGARVVRISALDDEELPPVDALYIGGGFPETHAGRLAANEPFRLCLKQAAEAGLPIYA
                     ECGGFLYLGESLTVGGKRYPMTGALPVAFGMEAKPQGHGYTTLAADAENPFFPVGTTI
                     NGHEFRYGRILSRRQEETHTAFRVLKGTGLDGQREGLCRKNILAGFTHVHALGTPEWA
                     KAMIAKAFQYREEKRNEECTINIMRRF"
     gene            complement(5993..6217)
                     /locus_tag="PLR47_05315"
     CDS             complement(5993..6217)
                     /locus_tag="PLR47_05315"
                     /inference="COORDINATES: protein motif:HMM:NF020263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory sulfite reductase D family
                     protein"
                     /protein_id="HOG12396.1"
                     /translation="MSDDLKAKITELFREKSKGDKKMFYIRDVTKWLPGEDRHAIQNT
                     VKELLDEEVLKYWSSGSSTYLILTEFFPKE"
     gene            complement(6220..7284)
                     /gene="dsrB"
                     /locus_tag="PLR47_05320"
     CDS             complement(6220..7284)
                     /gene="dsrB"
                     /locus_tag="PLR47_05320"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - hydrogensulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     beta"
                     /protein_id="HOG12397.1"
                     /translation="MAARTDIGPPNYKTMLPPVIERNYGDWLYHEILKPGVLMHVSKS
                     GETLYSVRGGSPRLISTDHIREICDIAEKYCDGYLRFTSRHNIEFLLDDKDRVEPLIA
                     ELKDRGYPVGGTGHSISNIVHTQGWVHCHTPATDASGPVKALMDEIYDHFVEAKLPAH
                     VRIALACCLNMCGAVHCSDIAILGIHRTVPKTDNSRVPNVCEIPSTVASCPTGAIRGD
                     MKNKTVTVNEEKCMYCGNCYTVCPAMPIADPDNDGISIWVGGKVSNARSAPMFSKLAI
                     PFLPNNPPRWPEVVNAVKNIIEVYASHARKHERVGEWIERVGWERFFSLTGIPFTDQH
                     IDDFDLATETFRTSAQFKWR"
     gene            complement(7286..>7581)
                     /locus_tag="PLR47_05325"
     CDS             complement(7286..>7581)
                     /locus_tag="PLR47_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12398.1"
                     /translation="VGGKAPIVRGALLSWVIVPFIKLEPPYDELKNLIERIQDWWDEN
                     AKSRERLGELIQRLGMRVFLKAVDLPAVPQMVKAPRTNPYVFFWPEDLKKEVK"
BASE COUNT         1633 a         2198 c         2145 g         1605 t
ORIGIN      
        1 ccgagcagcg gcggcactcc gtgcattggt aaaagtagta aaaccattcc ttgagtacat
       61 cgaccgtcag gtcgcgcgcg cccgcgagcg tcccgaagca catccccgcc tttgtgaagt
      121 agcggcggta aacggaccgg atcagctcgg cgcgcaggac aggcatgttt ttcggatcgc
      181 cggagccgat gtagaaatgg cacttgtccg cgcaggcgcc gcagcgcacg cagatgtcca
      241 taaacaggcg gaaggagcgg tatttttcga gccgctcttt catgccgtca agaatgatct
      301 gtcgccagtt ttccggaagc ttccaatcgg agtcggatgg ctgccaggcc cgcggattgg
      361 gcatgtccag ggccgccaga ttctttgcgg cggcagcgta gctccacgtt cccggacgga
      421 aatcgaccgg agttttcatc caggcggtgg ccggcggctt gtatccgatc ttcatcagtt
      481 cttccggttt gggaattttc gcgagcgaca tggctactcc ttctccaccg gcaggccggc
      541 cgctttcatt ttatcccgga actcatcttc atattcttta taggtgtgta ccttcacagg
      601 ggtgttccag ggattgatat gacgcaggct gcgattgttg ttgatcatgt tgcgcgtcgg
      661 gctcataaaa atgccgccaa gatgcatcag tttgctgaaa ggcagccagg caatcaggat
      721 aaagacgaaa caaaggtgca tgtaaaggat gacgcttccg tctttgggcg caaccggcgc
      781 aaacgttgtc cagcccatcg ccatggtctt cacggcgttg agatcaaccg gaaagaaatg
      841 gcgcatcaaa aggccggttg ccgcaacggc cgcaatcaaa agcagtgcga aataatcggc
      901 aaacagtgaa atgtagcgca cctgcggcag gaccgcccgc cgcaggaaga gaaacgccag
      961 tcccgcaagc agcgttgcgt cgctcaaata aagcgccgga acgccgattt ccagaaagcc
     1021 gtccagcgcg gaaagacccg tcacggcggg aagaaccggt tcggtgaaaa agcgcagatg
     1081 ccgaaggacg acgaccagaa gcgagccgtg aaacatcagc ccgaaaaacc acagccattt
     1141 ggcgctgccg taaaccggcc ggccgtccac gatttccgtt tgcgaatttc tgaacaaaga
     1201 acgaaacaaa aagatttctg cggccatccg gccgatcgcg cccagcggcc ccgcgggatt
     1261 atccagtgga ttgcttttga tccagggcag gcttttcccc tggccgcaag tcgcgggaat
     1321 gcgaaaggga acgggagctt tcgcccaccg gataacgcgg taaacaaaac cagccaaaaa
     1381 cagaccaaac gccgcatagg gaagaatcct gaaccatccg ggcgcgtccg cccgcccggc
     1441 cgccgcggcc gcaagggcga ttgccagaat cgcgatgaag gacagcaaaa tgttcacgct
     1501 ttgtttcatt tcatttgccc gttttcattt ccatgacggc tcctcgccga aatggatgat
     1561 cggttttttc attccttcga gcgcgttgac catcagttca accagaccgc cgtaataaag
     1621 atggcttcgt tcttccgcat gataataggc gatcatatcg cggatggcgc ctttgcagtt
     1681 ggagcaggga gcgcagacgt acttgggcgt ttccgccggc tcgctttcaa aagcttggag
     1741 gatctgcaga aacttctttc tggaactgac gaggttgcgc cactcggcaa aattgtatcc
     1801 cgacatgatg gcaaaaccgg acccgccacc gcagcagtag ttgttcacgc cgtgcggcgt
     1861 catttcccgg aagcgcggag cgatttttcc aaggattctt ctttgcggcg ccacgatgcc
     1921 catcgaccgg acgacattgc agggatcatg cagtgtgacg ggaaaatcat tcttttgcgg
     1981 attaaacgtc agttttccgc tcatcacgat atcctcaagc gtcaccagcg ccgactcccg
     2041 cggaatgttc aggtctccgg tcagcaaacg gtcggcaaca accgtcagtg ccttgtgcgc
     2101 atggccgcat tcacccagga cgatcttctt gactttaagc cgacgggcaa tgtcggcatg
     2161 tttgacggcc acacgagcaa attgaacgtc gtcgtaccaa aggccgtagt tgacggcgtc
     2221 gtagccggcc gcttcggacg acagcgtcca ggacagcccc gccgcctgga agaggattgc
     2281 aaacgctccg acgttttccg gccaggccag catttcgccc gcgttatgca gaagcagaat
     2341 atccgctcct tgcacgtccc aggggatttc gactttgcag ccggtgatct cttccatttc
     2401 ttcctgaatg aattcgacgc tgtcttttgc cgccagcgga ttcatgcccg tcgaagatcc
     2461 ggcccgcaga tgcagaacgg tccccttctc gtgaagttct ttgggcgcga tgcccatctc
     2521 ctgagaaaag agcttgcgga tctcgtgcgc aatgagaccg ttgtccacgc cgatggggca
     2581 aacggacgcg cagcgccggc acagagagca gcggtaggaa agttccgcga ggcgtgcgat
     2641 ggccttccag ttcaagtcga tgtcgccggt ccattttgcc gttattcttc cacccggctt
     2701 gagatatttt ttatagatcc ggcgcagcac ttcggagcgg aaaatcggcc ggtaaatttc
     2761 ctgccgtccg ctcatttcaa aaacggggca ggcctccgaa caggtctgac accgggcgca
     2821 atgctccatg gacaaaagca ggggctgcag gaaggtccag ttattttcct tctcaaacag
     2881 ttttttcagg ccggcaagaa atcggcgtac cagttcctct tcttcttgct tgtttgcggg
     2941 ctcggcaatg ccgatagcca gcgtgtcgtc gggcgacgtt tcgaggcctt tgatcttctc
     3001 cgccgccaga ggcttcaaaa cggcctcgtt gaagtcctcg taaggcggcg gcagcggtgc
     3061 gagatcgccc ggttcaatgc ggcacaggcc ggcggagcct cttgagatat cggcgggcgt
     3121 tatgcggtct ttcatttcat ctccttgggc cccgccgaag cgatatcctg ccaggttttc
     3181 tttccgtccg cgtcaacgtg cgcggctttc catgtcggcc ggtactgcag attcctccgt
     3241 atggccgatg cgatgggtga tccgggagtg ttcggcgaat cgtcccattt caccttgtga
     3301 tacaaaaaaa acttcatgaa catgtgcgat agatgcgtca gcggaatata ggccgcaaga
     3361 agcgagccca gcaatatcgt aagagctcca aagaggcttt gtccgggaac gtaggcggga
     3421 gcggccgcgc cggcggtcag caggccgagc acataaagcc tggccgcggg gaagcaggga
     3481 tcgaccgcca gcgcagagag aagggcacag acaaaaaaca gcaaaataaa gacgagattg
     3541 aaataatcgg ccggcgttgc ataggcccgg agattccggt cggcaaggcg tttgtacagc
     3601 atgcccaggg acccggcggt gccggccagg atgccgatcc acccggcggc ggcgatcagc
     3661 acattgatga tcgatgaaac agcgctgtct tcggcaagcc ccagcaaaaa caggacggcg
     3721 gacaacaaaa gcagcgcaaa aaccaccagc atcaggtaca ttcccgcgtg aaagggaaag
     3781 gaaaacagcc acaggttccg attttccttc cacaagcccc gcatgagccc gatttccggc
     3841 gccatgtaaa gaagttcgtt cacaggtgag cgctcccgct ttttcttcca ccagttcagc
     3901 tcttccatgt aagaaccgcc gtaagccgcc ttatttgccg gttcgtgctg caccgggtag
     3961 atttcccagc gcacatgcac gggaagcgtc agctgccggt atacgcggca ggcaacggca
     4021 accagaaaaa tgaccgtgca taagtatgaa aaaatataaa atcccatatc aatccgtcag
     4081 tttaatattt cttttccaaa tcaagatgat accgcggcgg caaggaggag gcgccggagg
     4141 cgtattttta acacgtcgag gacgccgaca acgcagccaa caaaggtagc gcttgatttc
     4201 ggaaagaaat caaacgcagc ccgtcggctt ggacaacccc gccagtttgc acgctccctt
     4261 tgccggtccg gtcgggaaaa gttcataaag cttcttcata tcgatcccgt tgtccttgca
     4321 gactttgcgc accatcgggg cgatgccgaa cttcgcaaaa tagtcgcgca ggtagttgat
     4381 gatcttccag tgctcttccg tcagcggtcc ttcaatgtct tcggtgtgcg cataggcctg
     4441 cgcgaattct tcgttccatt tatcgagttc ctgcagaaac ccgtcttcgt ccacttcgta
     4501 aattttgcct gctaattcga tctgcggcat ggttaaaacc tcctcatgat atttatcgtg
     4561 cattcttcgt ttcgtttttc ttcgcggtac tgaaaggctt tggcgatcat ggcctttgcc
     4621 cattcaggcg ttcccagcgc atgcacgtgg gtaaagccgg ccagaatatt tttccggcac
     4681 agcccttcgc gttgtccgtc gagccccgtc cccttgagga cgcgaaaagc ggtgtgcgtt
     4741 tcctcctggc ggcgagacag aatccggcca tagcggaatt cgtgtccgtt aatcgtcgtc
     4801 ccgacgggaa agaaagggtt ttccgcgtcg gcggcaaggg tagtgtaacc gtgcccctgc
     4861 ggtttcgctt ccatgccgaa cgccaccggc agagccccgg tcatgggata ccgcttgccg
     4921 ccgactgtca gggattctcc caggtagaga aatccgccgc actccgcgta aatcggcagg
     4981 ccggcttcgg ccgcctgttt gaggcagagg cggaacggtt cgttcgcggc gagcctgccg
     5041 gcgtgcgttt ccggaaatcc tcccccgatg taaagggcgt caacgggcgg cagctcctca
     5101 tcgtcaagcg cgctgatccg gacgacccgt gcgccccggt cggcgagcgc ttcgatattt
     5161 tccgcataat aaaactgaaa cgccgcgtcc gaaatcacgc cgatggtgac gggagggtcc
     5221 atgtccctca cggacggagc cgctttcgga aacgctccga ccttccaggg cggcgcgctt
     5281 cgcgcgatct tcaccaggga cggaatgtcc agatagtcgc gggcgatggc gccggccgtg
     5341 tcgagcgcct gctgaacatc ggcgtgctcc gcggggggaa tcaggcccag gtgcctttgg
     5401 acaaagggca gatccggcag acgcgggacg gtcccataga ccggaagccc ggtggattct
     5461 tcaacggccc tccggatcac ggacgcatgg cgttttccgt tcacccggct taaaatcacg
     5521 ccgcggatgt caaccgcggg atcaagatgc aaacagccca gcaccatcgc cgccgccgta
     5581 cgcgtgactt tgtcgcagtc tacaatcagg ataaccggcg catagagcag tttggccagt
     5641 tcggcggtgc tgaattcgcc ggcggcgttg acgccgtcaa acaaacctcg gttgccttcg
     5701 atcagagaaa aggttccttc cgcggcatgg cgcgaaaaag atcggaccac ctgcctggct
     5761 cccatgagat acgtatccag attgtagcag ggaccggatg ccgcccggcc aagccaggcg
     5821 gcatcaatat aatccggtcc ctttttgaaa ggagtgacca tcaggccctg ccggcgcaga
     5881 gcgaccgtca cagccaaaga aagggtggtc tttcccgcgc cgccccgcag ggcggccatg
     5941 atgaagcgcg ggcgttgttg attcatatcc gttacctctt gtaaaacagt ccctattcct
     6001 ttggaaagaa ttccgtcagg atgaggtacg tcgagctgcc gctcgaccag tacttcagca
     6061 cttcctcgtc gaggagttcc ttcacggtgt tttgaatggc gtggcggtcc tcgccgggca
     6121 gccatttggt gacgtcccga atgtaaaaca tttttttgtc gcccttcgat ttttcgcgaa
     6181 acagctccgt aattttcgcc ttcagatcat cactcatggc tacctccatt taaactgggc
     6241 ggacgtccgg aaagtttccg tcgccagatc aaagtcatcg atatgctgat ccgtaaacgg
     6301 aatgcccgtc aggctgaaga aacgctccca gccgacgcgc tcgatccatt cgccgacgcg
     6361 ctcgtgtttc cgggcgtggc tcgcataaac ctcgatgatg ttttttaccg cgttcaccac
     6421 ttccggccag cgcggcgggt tgttgggcag gaagggaatc gccagcttgg aaaacatcgg
     6481 agcactcctc gcgttggaaa ctttgccgcc gacccagatg gagatgccgt cattgtccgg
     6541 atcggcaatc ggcatggccg ggcagacggt gtagcagttg ccgcagtaca tgcacttctc
     6601 ctcgttgacg gtcacggtct tgtttttcat gtcgccccgg atcgcgcccg tcggacaaga
     6661 cgccaccgtg gagggaattt cgcagacatt cggcacgcgc gagttgtcgg tcttgggaac
     6721 cgtccggtga atgcccagaa tggcgatgtc cgagcagtgg accgcaccgc acatgttcag
     6781 gcagcaggcc agggcaatcc ggacatgcgc cggcagcttg gcctccacaa agtgatcgta
     6841 aatttcatcc atcagcgctt tgaccggacc ggaagcatcc gtggccgggg tatggcaatg
     6901 gacccatccc tgcgtatgaa cgatgttgga gatcgaatgt cccgtgccgc ccacgggata
     6961 gccgcgatcc ttcagttccg cgatcagcgg ttcgaccctg tccttgtcat cgagaagaaa
     7021 ttcaatgttg tgacggctgg tgaagcgcag atagccgtcg cagtattttt ccgcgatgtc
     7081 gcagatttca cggatgtgat ccgttgaaat caaccggggc gaaccgccgc gcacgctgta
     7141 gagcgtttct cccgacttcg acacatgcat caggacgccg ggcttcagga tttcatggta
     7201 gagccagtcc ccgtaatttc tttcgattac gggaggcagc atcgttttat agttcggggg
     7261 gccgatatca gttcttgccg ccattttact tcacctcctt tttcagatct tcgggccaga
     7321 agaacacgta gggattggtt ctcggcgctt taaccatctg gggcacggcg ggaaggtcca
     7381 cggctttcaa aaagacacgc atgcccaggc gctggatcag ctcacccagc cgttcgcgcg
     7441 atttcgcgtt ctcgtcccac cagtcctgga tccgttcgat cagattcttc aattcgtcat
     7501 aaggcggttc cagctttatg aacgggacga tcacccagga gagcagggcg ccccggacaa
     7561 tgggcgcttt gccgccgacc a
//
LOCUS       DAOSIM010000092         7502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_9026_length_7502_cov_8.541292, whole genome shotgun sequence.
ACCESSION   DAOSIM010000092 DAOSIM010000000
VERSION     DAOSIM010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7502
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9026_length_7502_cov_8.541292"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLR47_05330"
     CDS             <1..185
                     /locus_tag="PLR47_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12399.1"
                     /translation="GKEKNLRQLLPLGMIAGANSLMTGNYLTTTGRDSRLDHEMIIDL
                     DLVPTRPLDICKCGQD"
     gene            complement(182..1018)
                     /locus_tag="PLR47_05335"
     CDS             complement(182..1018)
                     /locus_tag="PLR47_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF128 domain-containing protein"
                     /protein_id="HOG12400.1"
                     /translation="MTGFSEVSLMYDSPYSASSDVSNARAQDNVDLSISRIEVLAFRT
                     TFDVKKRRGMVPVNVSFFPEKQFARALKIMRPVFAKNLAVSSRVAVAKAGESLGDVVV
                     PEGKTGLATICSIVVNGVLLKHGIPVDSKFGGILQLRKGQGLRFVELIHYASSSLDPS
                     EIFIRGKMTSIGQVVANGDGKILANFREIPALSRTLVNCVIDDFTRAGINAVLSIGDI
                     GHPICQTHVDLNKIGMIMIGGLNPVVAVSEAGIPVDNRAMSTVMEFEQLQDIGKGKGS
                     NL"
     gene            complement(1137..2717)
                     /locus_tag="PLR47_05340"
     CDS             complement(1137..2717)
                     /locus_tag="PLR47_05340"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HOG12401.1"
                     /translation="MSFSRVNTSSATLTKNRTEESISPTSGMCVTCIDGCVGMCEIGK
                     SAYRGHEVIYPQPFGVITCAGEKKYPVDYSHFNIMGTAAGAYGIEADSDKAIFPAVNL
                     EVVFGHDKGIKFKLPWLISGIGSTNIAKNNWEGLAIGSALAGTGLTIGENVVGMDPQS
                     KFKNGNILDTVDLKRRVKLYRDYQRFGYGAIIVQANLEDTRLNVHEYAIEELGVECVE
                     LKWGQGAKDIGGEVKIKDLKKAQMLHDRGYIVLPDPYDSNVIEAFARGAFKEFERHSR
                     VGMVTEESFAATIKRLRKAGAKYIFLKTGAYRPVDLARAIYFSSKYKLDLLTVDGAGG
                     GTGMSPWRMMNEWGVPPVELHTLVYQYAKKLAAKKKYLPAIAVNGGFTFEDQIFKGLA
                     MGAPFVKLIGMARAPIAAAMVGKTIGQTIDAQQVPVYVERFGNTKDEIFVTASELRHE
                     LGHKEFEQLPTGAIGLYTYYERLAQGLRQLMAGSRKFALKYMTRDDLAALTREAADVS
                     GIQYIMDVDKKEAEKILK"
     gene            complement(2745..3416)
                     /locus_tag="PLR47_05345"
     CDS             complement(2745..3416)
                     /locus_tag="PLR47_05345"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOG12402.1"
                     /translation="MVNLTLNGLSISVERGTTILEAAKFYGFPIPTLCHKEGLSPYGA
                     CRLCVVEIGEGPGAKLVSSCTYPVEEGMKVRTASERVLKARRIIIELLLASCPQSKVI
                     QDIASRHNVRQQRFRQEHEDCILCGLCVRMCNEQMMAGAIGFRGRGKTRSLGTPFDMK
                     SDVCRLCGGCMEVCPACQARCTFNQPEKAICGGCANLAPPCIAKDQFHDMMCYMSPCV
                     ACEIK"
     gene            complement(3409..6657)
                     /locus_tag="PLR47_05350"
     CDS             complement(3409..6657)
                     /locus_tag="PLR47_05350"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOG12403.1"
                     /translation="MKRFQSVAQFKTYRKTFLATSDQSKPCIVICGGTGGQASGSNDL
                     IRIIKRQILEKDLHDKLDLRITGCLGFCEMDPFIIVEPGYNLYPKLKMADVPRIIDAA
                     VRGKVVEELLYREPGSTVKRYSEKEIPFFQGQTRIVLGQNQRLDPIRIHDYLKIGGYA
                     ALEKALQKKDRAWLVDEILNAKLRGRGGAGFLTGKKWDLARSMKSRGPKYIICNADEG
                     DPGAYMDRSMLEGNPHAVIEGMMIAALAIGATHGIIYVRTEYPLAIKHATIALRSVRQ
                     LGLLGKNILGQGVDFDIEIFHGAGAFVCGEETALIKSIEGKMGEPRQRPPFPIQKGLF
                     GYPTCINNVETLSNVPYIINHGAAEYLKIGVENNAGTKIFSLVGKIKNTGLVEVPLGT
                     PIRKVVYDIGGGPSGEAKIKAIQTGGPSGGCIPASMFDLNIDYDSLAKAGSIMGSGGM
                     IVMDQNTCMVDVAKYFMSFLKDESCGKCFTCRKGTQRMYEILEDITEGRGTPGHLQLL
                     EELAQTVKDTTMCGLGQTAANPVLSTLRYFRDEYERHIADKRCEAFACKELTGAPCQA
                     ACPVDTEPWRYVALVEKGDYEEAYRVIRSANPFPAVSSRVCDRKCESRCNLGVSGGES
                     IGIRAIKRFVTDRVDPSAYRPKPAKNKGKEVAIIGAGPAGLTAAHNLSILGYKVTVFE
                     AEQVPGGMLSCSLPAYRLPRDIVEKEIQTLLNGNIKIEYGRTLGKDMSLQDLLSKKFS
                     AVFLSIGAHESWRLDLENEDIEGIYPSIQFLKAFNIRGEQLARGHVGIIGGGNSAVDA
                     ARVALRQKDVTSVSLFYRRTREEMPAYEEEIDAALEEGVRIETLISPVKIHVRQEEID
                     AALREGVELETLVTPVKIYSKEGRVVGIECIKNRLGDVDATGRRKPVPIPGSEFKVEL
                     DTLIVAIGEKPQSEFLSAMGLDVDKGGRIRVNPKTFETSLKGVFAGGDLVTGPNTVID
                     AIAAGKKAAQCMDRYLQNQPLVVPASPRRPGVFIEPAVIDEAEAQTATRAEPAVLPPS
                     QRLKNFREVTMVLPEEQARSECRRCLRCDLEFTKKQSAEKPAAAAIGGGKNG"
     gene            complement(6675..>7502)
                     /locus_tag="PLR47_05355"
     CDS             complement(6675..>7502)
                     /locus_tag="PLR47_05355"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOG12404.1"
                     /translation="IRKHDKNKGALIAILEEIQAAYTYLPEEALRLVSKETGRSLVDI
                     YGVATFYKAFSLKPRGKHCVSVCLGTACHVRGAQGIVDEFKRELDIAPGETTADNEIT
                     LETVNCLGACALGPTVVVDGHYFPHVSKSKVKNIIARAMAGLEKIDAETDNRVFPLQV
                     SCSACNKSLLDPEYPMDGYPSINLRVSCNGTRGRLRLSSLYGSGASVLEHPIPSNSLS
                     RFYCPHCAGELPSFSTCTECGSPMAAMRVCEGCAWEVCTRQGCEGHLLNLNQMNIQK"
BASE COUNT         1488 a         2225 c         2040 g         1749 t
ORIGIN      
        1 gcggaaagga aaaaaatctg cgccagcttc tgccgctggg catgatcgcg ggcgccaatt
       61 cgctgatgac gggcaactac ctgaccacga ccggccgcga cagccggctg gaccacgaaa
      121 tgatcatcga tctggatctt gtccccaccc ggccgctgga catctgcaaa tgcggccaag
      181 attaaagatt tgaccccttg cctttgccga tatcctgcag ctgctcgaat tccatcacgg
      241 tggacatggc gcgattatcc actggaatgc cagcttcgct gactgccaca accgggttta
      301 atccgccaat catgatcatt ccgatcttgt tcagatcaac atgcgtctgg cagatgggat
      361 gcccaatgtc cccgatggat aagacggcgt taatgccggc gcgcgtgaaa tcatcaatca
      421 cgcaattcac cagtgtgcgg ctcaaggcgg ggatttcacg gaaattcgcc agaattttac
      481 catcgccgtt cgccaccact tgcccgatgg aggtcatctt gccgcgaata aaaatttccg
      541 acgggtccag cgatgagctg gcgtaatgaa tcagctccac aaatcgcagg ccctggcctt
      601 ttctcagttg aaggattccg ccaaatttgg aatcaacggg aatgccgtgc ttgagcagca
      661 cgccgttgac cacaatgctg caaatcgtcg ccagacctgt tttcccctct ggcacgacga
      721 cgtctcccag cgattctccc gccttcgcca cggcaacccg tgaactgaca gcgagatttt
      781 tggcgaagac gggcctcatg attttcagcg cccgggcaaa ctgcttttcc gggaagaagg
      841 aaacattgac gggaaccata ccgcgcctct ttttaacatc gaaggtggtt cgaaaggcca
      901 ggacttcgat gcgtgaaatg gaaaggtcga cgttgtcctg agcgcgtgcg ttggatacat
      961 ccgacgacgc ggaataaggc gagtcataca tcaatgagac ctcactgaac ccggtcatga
     1021 agctatcctt tttaaacggc atccatcggc attcgcgcgc tcacccctcc tgacggggca
     1081 agcgcgtttc accgatatcc ttgcatgatg tattattctt aatctccggc cggcggctat
     1141 ttgagaattt tttcagcttc tttcttatcg acatccataa tatactgaat gccgctgaca
     1201 tccgccgctt cacgggttag tgccgccagg tcgtcccgcg tcatatattt cagcgcaaac
     1261 tttctgcttc ccgccatcaa ctggcgaagc ccctgcgcca gtctttcata gtaggtgtaa
     1321 agtccgatgg cgccggtggg cagctgttca aattccttgt ggcccagttc atggcgcagc
     1381 tcgctggccg tgacgaaaat ttcatccttc gtattgccga agcgttcgac ataaaccggc
     1441 acctgctggg cgtcgatggt ctggccgatc gtcttaccga ccatggccgc cgcaatgggc
     1501 gcccgcgcca tgccgatcaa tttgacaaag ggcgcgccca tcgccagacc tttgaatatc
     1561 tgatcctcga aggtaaagcc gccgtttacg gcaatggccg gcagatattt tttcttcgct
     1621 gcgagctttt tggcgtattg atacaccagc gtatgcagtt ccaccggggg gacaccccac
     1681 tcgttcatca tccgccacgg gctcatgccc gtaccgccgc cggcgccgtc cacggtcagc
     1741 agatccagct tgtatttcga cgaaaaataa atagcccgcg ccagatcgac gggacggtag
     1801 gcgccagttt taaggaaaat gtacttggcg ccggccttgc gcagcctctt aatcgtcgcc
     1861 gcaaacgatt cctcggtgac cataccgacg cgcgaatgac gctcgaactc cttgaaggcg
     1921 ccccgtgcaa aggcttcgat gacattggaa tcatagggat ccggcaaaac gatatagccg
     1981 cggtcgtgga gcatctgggc ttttttcaaa tccttgattt tgacttcgcc gccgatgtct
     2041 tttgcaccct gcccccattt aagctccacg cattccacgc ccagttcctc aatggcgtat
     2101 tcatgcacat tcaaccgggt atcctccaga ttggcctgca cgatgatggc gccgtagccg
     2161 aagcgctgat aatcccggta aagcttgacg cggcgcttca aatcgaccgt atccaagatg
     2221 ttgccgtttt tgaatttgga ttgcgggtcc atcccgacga cgttttcccc gatggtcagc
     2281 cccgttcccg ccagggcgga cccgatggcc agtccttccc agttgttctt ggcgatgttg
     2341 gtggagccga tgccggaaat cagccaggga agcttgaact ttattccctt atcgtggccg
     2401 aaaaccactt ccaggttgac cgcggggaaa atggccttgt cgctgtcggc ttcaatgccg
     2461 taagcgccgg cggccgtccc cataatgttg aaatgggaat aatccaccgg atatttcttt
     2521 tcgccggcgc aggtaatcac gccgaaaggc tgggggtaga tcacttcatg gccgcgataa
     2581 gcggatttgc ctatttcaca catgccgacg cagccgtcga tgcacgtgac acacatgccc
     2641 gacgtcggtg atatagactc ttctgtcctg tttttggtaa gggtggcgct ggaggtattt
     2701 actcttgaaa aagacatata tcctgctcct ttctgggttt aagcttattt tatttcacag
     2761 gccacgcagg gtgacatgta acacatcata tcgtggaact gatccttcgc aatgcacggc
     2821 ggcgcaaggt tggcgcagcc tccgcagatc gctttttccg gctgattgaa cgtgcagcgc
     2881 gcctgacagg ccgggcagac ctccatgcag ccgccgcaca gccggcagac gtcggatttc
     2941 atgtcgaaag gcgtgcccag gcttctcgtt ttgccccgtc cgcgaaaacc gatggctccg
     3001 gccatcatct gctcgttgca catccgcacg cacagtccgc agaggatgca gtcctcgtgc
     3061 tcctgccgga agcgctgctg gcgcacgttg tgccgcgacg cgatatcctg aatgaccttg
     3121 gattgcggac aggaagccag gagaagctca atgatgatcc ttctcgcctt caggacgcgt
     3181 tccgaggcgg tgcggacctt catcccttct tcaacgggat aggtgcacga cgagacgagc
     3241 ttcgcgcccg ggccctcgcc gatttcgacc acgcaaagcc ggcacgcgcc gtaaggcgag
     3301 agcccctcct tgtggcacag cgtcggaatg gggaaaccgt aaaatttcgc cgcttccaga
     3361 atcgtcgtcc ccctttcaac ggatatggac agtccgttca gtgtcagatt aaccattttt
     3421 cccacctccg attgcggccg ccgcaggctt ttccgcggat tgttttttgg tgaattcaag
     3481 atcgcatctc aggcaccgcc ggcattccga ccgggcctgc tcttccggca ggaccatggt
     3541 gacttcccgg aagtttttca gcctttggga cggcgggagc acggcgggct cggcacgggt
     3601 tgccgtttgc gcttccgctt cgtcaataac ggccggttcg ataaagacgc cgggacgtct
     3661 cggcgaggca ggcacaacca gcggctggtt ttgcaaatat cgatccatgc attgcgcggc
     3721 ttttttgccg gccgcgatgg cgtcaatgac ggtgttgggt ccggttacga ggtcgccccc
     3781 cgcaaaaacg cccttgaggc tggtttcgaa ggttttggga ttgaccctga ttctcccgcc
     3841 tttatccacg tccaggccca tcgcggacag aaactcgctt tggggctttt cgccgatggc
     3901 gacgatgagc gtgtcgagtt ccaccttgaa ctcgcttccg ggaatcggaa cgggtttgcg
     3961 ccttccagtg gcatccacat cgcccagacg gtttttgatg cactcaatgc cgacgacacg
     4021 gccttctttt gaatagattt taaccggcgt gaccagggtc tccagttcaa cgccttcccg
     4081 aagcgccgcg tcgatttcct cctggcgcac atgaatcttg accggcgaaa tcagggtttc
     4141 aatccgcacg ccttcctcaa gggcggcgtc gatttcttct tcataagccg gcatttcttc
     4201 gcgggtccgc cggtagaaaa gcgacacgct ggtaacatct ttctggcgca gggcgacacg
     4261 cgcggcgtcc accgccgaat tgccgccgcc gatgattccc acatggcccc gggccagttg
     4321 ttcgccccgg atgttgaacg ctttcaggaa ttgaatggac ggatagattc cttcgatgtc
     4381 ttcattctcc agatccagac gccagctttc atgcgcccct atggaaagaa agaccgcgct
     4441 gaattttttg gaaagcaggt cctgcaggga catgtctttt cccagcgtcc ggccgtactc
     4501 tattttgatg ttgccgttga gaagtgtctg gatttccttt tccacaatat cccggggcag
     4561 ccggtaagcg ggcagagaac aggacagcat gccgcccggc acctgttccg cttcaaagac
     4621 ggtcaccttg tagcccagaa tagaaagatt gtgggcggcg gtgaggcccg cggggccggc
     4681 gccgattatg gccacctctt ttcccttgtt cttcgccggt ttcggccggt aagcggacgg
     4741 gtccacccgg tcggtgacaa accgcttgat ggcgcggatg ccgatggact ctcccccgct
     4801 tacgcccagg ttgcagcggc tttcgcattt gcggtcgcag acccgcgagc ttacagccgg
     4861 aaaaggattc gccgaacgga tcacacgata ggcttcttca taatcgcctt tttccaccag
     4921 ggcgacatag cgccacggtt ccgtgtccac ggggcaggcc gcctggcacg gggcccctgt
     4981 cagttccttg caggcaaacg cttcgcagcg cttgtcggca atgtggcgtt catattcgtc
     5041 acggaaatac ctaagtgtgc tcaggacggg attggcggcc gtttgaccga gcccgcacat
     5101 ggtcgtgtct ttcacggttt gcgccagttc ttcgagcagc tggagatggc ctggcgtgcc
     5161 ccgtccttca gtaatatcct ccagaatttc atacatccgc tgcgtgccct tgcggcaggt
     5221 gaaacacttc ccgcaggact cgtctttcag gaagctcata aaatattttg cgacgtccac
     5281 catgcaggta ttttggtcca tgacaatcat gccgcccgat cccatgatgg aaccggcttt
     5341 ggccaggctg tcataatcga tgttcagatc gaacatggag gccggaatgc acccgccgga
     5401 cggtccgccg gtctgaatgg ccttgatctt tgcctcgccc gaagggccgc cgccgatgtc
     5461 atagacgact ttgcgaatcg gcgtcccgag cggcacctcc accagaccgg tgttcttgat
     5521 cttgccgacc agggagaata tcttcgtgcc ggcattgttt tccacgccga tcttcaaata
     5581 ttcggcggcg ccgtggttga tgatgtaggg cacgttggac agagtctcaa cgttgttgat
     5641 gcaggtcgga tagccgaaaa ggcctttttg aatcggaaag ggcggacgct ggcgcggctc
     5701 tcccatcttt ccttcgatgg atttgatcag cgccgtttcc tctccgcaga caaacgcgcc
     5761 cgcgccgtgg aagatttcga tgtcgaaatc gactccctgc cctaaaatat tttttcccag
     5821 aagccccagt tgccggacgc tgcgcagcgc gatggtcgcg tgcttgatgg ccagcggata
     5881 ctccgtgcgc acgtaaatga tgccgtgcgt ggcgccgatg gccagggcgg caatcatcat
     5941 cccttcaatg acggcgtggg gattcccttc cagcatgctc cggtccatat aggcgccggg
     6001 gtctccttca tccgcgttgc agataatata tttcgggccg cggctcttca tgctgcgggc
     6061 cagatcccat tttttcccgg tcaaaaaccc ggccccgccg cgcccccgca gtttggcgtt
     6121 gaggatttca tcgaccagcc aggcccggtc ttttttctgc agcgcttttt ccagcgcggc
     6181 gtacccgcct attttcaggt agtcgtggat ccggatggga tcgaggcgct ggttctggcc
     6241 aagcacgatg cgggtctgtc cctggaaaaa gggaatttct ttttcggagt agcgtttaac
     6301 cgtcgaaccg ggttcccggt acaggagttc ttcgaccacc ttccctctca cggccgcgtc
     6361 gataatccgg ggaacgtccg ccattttcag cttcgggtac agattgtatc ccggttcgac
     6421 gatgatgaaa ggatccattt cgcaaaaccc caggcacccc gtaatccgca ggtccagttt
     6481 gtcgtgcaga tccttttcca ggatttggcg cttgatgatc cggatcagat cattcgagcc
     6541 gctggcctgt ccgccggtgc ccccgcagat gacaatgcag ggcttggact gatccgaagt
     6601 tgccagaaat gttttgcgat acgtcttgaa ttgagcgaca gattgaaacc tcttcatctc
     6661 ttttcccttc atcgttattt ttgaatgttc atttgattca gattcagcag atggccttcg
     6721 cagccctgac gcgtgcatac ctcccaggcg cagccttcgc agaccctcat ggcggccatg
     6781 ggggaaccgc actccgtgca ggtagagaaa ctcggcagtt cgcccgcgca gtgcggacag
     6841 taaaatctgg acagactgtt tgacggaatc ggatgctcca ggaccgaggc cccgctgccg
     6901 taaagcgaag acaggcgcag ccggcctctc gtgccgttgc aggatacacg gaggttaatc
     6961 gagggataac cgtccatcgg atactccgga tccagaaggc ttttattgca ggcggagcag
     7021 gaaacctgga gggggaatac ccggttgtcg gtttccgcat cgattttttc cagaccggcc
     7081 atcgccctgg cgatgatgtt tttcaccttg cttttgctca catgggggaa ataatgcccg
     7141 tccaccacca cggtcggtcc cagagcgcag gcccccagac aattgacggt ttccagggtg
     7201 atctcgttgt ccgccgttgt ttcgccgggc gcaatgtcaa gctcgcgctt gaattcgtca
     7261 acgatcccct gagcgccccg gacatggcat gccgtaccca ggcagacgga tacgcaatgc
     7321 ttgccccgcg gtttcagact gaatgctttg taaaaggttg cgacaccgta gatatccacg
     7381 agcgagcggc cggtctcttt tgaaaccagc ctgagcgcct cttccggaag atacgtatac
     7441 gccgcctgga tttcctccag gatggcaatc agcgccccct tgtttttgtc atgcttcctg
     7501 at
//
LOCUS       DAOSIM010000093         7436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_9126_length_7436_cov_6.743937, whole genome shotgun sequence.
ACCESSION   DAOSIM010000093 DAOSIM010000000
VERSION     DAOSIM010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9126_length_7436_cov_6.743937"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLR47_05360"
     CDS             <1..304
                     /locus_tag="PLR47_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOG12405.1"
                     /translation="ANQTDDPEGKILFKALGKVEAEHASIWRKILKLGSVPPGSDACH
                     TKNVANLKESHEREIRAIEFYRKSAAEADHPRIRQLFEALVEIETDHLHLSEERLK"
     gene            541..3537
                     /locus_tag="PLR47_05365"
     CDS             541..3537
                     /locus_tag="PLR47_05365"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOG12406.1"
                     /translation="MIPDVRNSINPQKSLTALLMKMLLGIVAAGFQLRRSYHQYLKTS
                     EGWIDLTVGLRTESRSVECAIRFKDGRVSVLGRIPEKTDVTIVFRSDAVVKKLLAATP
                     SDQIFMLLKSEMRVEGSMMYLNLFFFLLSVLMNKKQIRQLQKEREASREAEIKKSPDS
                     RKELSDELQARRATRLKGEKVDGGVHYLEDPYLPEFGLDDFPRLKKFLDVHLTAKPEI
                     CPEMPRLLTEWHKKNGFETDAKGRPWEPATRKALSYKYLMENRAPVIAGDCLLAGTTT
                     SREVGCIVYPEGSGGLIWNELFTIAHRSYNPFEISEETRNLLHYEVFPYWAHRNFREW
                     VRDRYNNPLCMQIDERYAYYFNWKQATISHTIPDFPKMLRLGTSGIIGEIEQELKNVQ
                     GNAEKKATLEAMILTLEGLTAYSKNLAAQAKKDAAVQANPVRKKELERIAAVCSQVVE
                     KPARSLHEAVNAMWITWVGLHMESMNAGLSLGRLDQWLQPYFLADMQQLSSREDRAEY
                     IRDAIELIGHFYMRCTDHFPLTPDLANFYFGGSSSDQAITLGGVTPEGLDAVNDMTYI
                     FLKVTEMLSLRDPNVNARFTPGISSDAYLKRLCEVNLITAATPSMHNDKAMFAALSPM
                     GYDIRDIRNWSATGCVEPTLSGKHMAHTNMQMMNMVAALEMALNNGLHPLTDWKLGPE
                     TGAIEQGDFNSFEDFFNAFTEQFAFLIDRSIEYNDLLALAHQYLRPTPLLSSVIDGCI
                     TKGKDVTRGGATYNSSGTAIVGLADVTDSLMTIKKLVFDEKKISFAHLKKAVDGNFEN
                     DPELLAMVRRKVPLFGSGNAEAVEMANRLTRWSHDHYAGLTHYRGGKYTTGFWTMSQH
                     VAFGILTGALPSGRQAGKPFTPGLTPQPFASKNLLDNIRDVAKLDPVNLNNNIAFNVK
                     VVPSAADTREKTVHNMFSYVKTYFDLGGMQMQMNVVTSAMLRDAMAHPENYRNLIVRI
                     SGYNAYFVTLNKDMQRELIERAEYAM"
     gene            3548..4489
                     /locus_tag="PLR47_05370"
     CDS             3548..4489
                     /locus_tag="PLR47_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOG12407.1"
                     /translation="MAQTEESQARKPLILEIKGNSLDDGPGIRTVVFFKGCPLSCSWC
                     HNPESKLARVEIGYDRKECIGCGTCTAVCPEKAVSPQNIHPVDREKCTLCFRCVEECP
                     AGALARVGRSLSIDEIAAEVLKDKPFFDTSGGGATFSGGEPVMFMEFLSELLHALKAY
                     QVHTLLETSGFFDFEKFERLLLPCLDMIYYDIKIMDEKAHRIHTGVSNQRILENFERL
                     FKISRDTGITLLPRTPLVPGITDRHENIQGIARFLAELGVRNAALMPYNPLWHEKADK
                     IGADNPHRGHAVMAAWMEKEKVEACRRIFSEEGIQVV"
     gene            4801..5154
                     /locus_tag="PLR47_05375"
     CDS             4801..5154
                     /locus_tag="PLR47_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOG12408.1"
                     /translation="MEIKFKIWLEKDGHALFGEGRDQLLKAIDECHGLYGAAKKLKMS
                     YRAAWGKIKLTEERIGKKIVETGEDKKLHLTKDAKKILDEFEKLEKDVDGFLHARRST
                     FIVLKDPPTKSDEKK"
     gene            5459..>7436
                     /locus_tag="PLR47_05380"
     CDS             5459..>7436
                     /locus_tag="PLR47_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOG12409.1"
                     /translation="MKLAGAAGVATAMTAFPFRNMRAAWAFGDHPQEQLPYRITKRVP
                     QVCARACEADCAYNVVVGVDPATGLERALTLEGRPEDPVSNGKFCIKGMGFVDSMYDP
                     DRLMVSLKRTNKTKGTDVDPGWIIMKTEDAVNEIIARMKTYKPEEILMASPGDPYTNR
                     LCQSIGCTRSDQRTECFGTHYYINCLTLTNPPNKFYSSCYTPSHHVCGYDYDTCKYQV
                     WFGFDSFSKCGKAGMLNHWAAGKRNGGKIVMFNPVRTPMTDNFASEYYAIKPGTDLAV
                     ALAMINQIMAGKKYNKEFMTKYSDGAALVDVEKQTHLKTADGMFLAWSVKNKKAEPID
                     ECDDPALDGSYTVTLDGRQIKAKTVLQLLAEATKTYTPQWAAKISDVPAKAISKIARD
                     FAAAAPYALIPTYKRDAAGPNYANSWRLRHAIQILNSLAGSIDHEGGMLLLHDVKIPW
                     IDEISPPALPYPEQPSQPVDFRNEFPVTDDIYRKKDFSAPGHYGMVGWGLYKTNRAKV
                     AFFRNPHRGLFAMIHSQMFEAASQKLELVVDWNLYLDDLGYFCDYVVPAPHQFEEGKM
                     DLRLYYPKYPCIVGGSPVQKSPGDQIGWGGFAMKIGLAMAPKYWTTDGSGNPAKKIPT
                     NMGDVAVKKAGIADSAADFMKKGGLWIDKKEYQNY"
BASE COUNT         1870 a         2031 c         1995 g         1540 t
ORIGIN      
        1 cgcgaatcaa accgatgatc ccgagggaaa gattctgttc aaggcgctgg gcaaggttga
       61 ggcggagcac gcgtccatct ggagaaaaat cctcaagctg ggaagcgttc ccccgggaag
      121 cgacgcctgc cacacgaaga atgtcgcaaa cctgaaagaa tcccacgagc gggaaatccg
      181 ggccattgaa ttctaccgga agtcggctgc cgaagcggat catccgcgga ttcggcagct
      241 ttttgaagcg ttggtggaaa tcgaaacgga ccacctgcac ttgtcggaag aaaggctgaa
      301 gtaaatgaat cccgtggaac cgggggacac tatcccccgg tttcgtgcca tgaaatcttt
      361 ccatcagcca caggaagagc ttaaatattc tccgcggttt cgcgaacacc tctcactatc
      421 caactcttct tgacaagtcc cgtcctgtgt gtcatccatt gcaaccggag agcgaaactc
      481 aagaggttga ctgaaaaaac tgattcttct ggaaaccaga tttcacaggg agaccctgtc
      541 atgattcctg atgtccgcaa cagcataaat ccccaaaaat ccctgacggc gctgctcatg
      601 aaaatgctgc tcgggattgt ggcggccggt tttcaactcc gccggtctta tcatcagtac
      661 ctgaaaacct cggaggggtg gattgatctg accgtgggct tgagaaccga atcccgctcc
      721 gtggaatgcg ccatccgttt taaggacggc cgggtgtccg tgctgggccg tatcccggaa
      781 aagacggatg taacgattgt tttcagatcc gacgccgtgg tgaagaaact tctcgcggcc
      841 acgccctccg accagatttt catgctgctg aaaagtgaaa tgcgggtcga aggaagcatg
      901 atgtacctga acctgttttt tttcctcctg tccgttctga tgaacaaaaa gcaaatccgg
      961 cagctccaga aggaaagaga agcttccagg gaagccgaga tcaaaaaatc acccgattcc
     1021 cggaaggaat taagcgatga gctccaggca aggcgcgcca caaggctcaa aggcgaaaaa
     1081 gttgacggcg gcgttcacta tctggaagat ccctatctgc cggaattcgg ccttgacgac
     1141 tttccccggc tgaagaaatt tctggatgtc catctcacgg caaaaccgga aatctgcccg
     1201 gaaatgccgc ggcttctcac cgagtggcat aaaaaaaacg gttttgaaac cgacgcgaag
     1261 ggccggccct gggaaccggc aacccgcaaa gccctttcct acaaatatct gatggaaaac
     1321 cgcgcacccg ttattgccgg agattgtctg ctggccggaa ccaccaccag ccgcgaggtg
     1381 ggatgcatcg tgtaccccga gggaagcggc ggcctgatct ggaacgagct cttcacgatt
     1441 gctcatcgct cctacaatcc ctttgagatc tcggaagaaa caagaaacct tctgcactac
     1501 gaggtatttc cctactgggc ccatcgcaat ttccgggaat gggttcggga tcgatacaac
     1561 aatcctctat gcatgcagat tgatgaacgc tatgcctatt atttcaactg gaaacaggca
     1621 accatctcgc acaccattcc ggattttcca aagatgctgc gcctgggcac atccggcatc
     1681 atcggggaga tcgaacagga actcaaaaat gtgcagggca acgccgaaaa gaaggccacg
     1741 ctcgaagcga tgattctcac cctggaaggc ctcacggcct actcgaagaa tctggccgcg
     1801 caggcgaaaa aagacgccgc agtccaggcg aatcccgtcc gaaaaaagga actggagaga
     1861 attgccgccg tctgttcaca ggtcgtcgaa aaaccggcgc gcagccttca cgaagccgtc
     1921 aacgccatgt ggatcacctg ggtcgggctt cacatggaga gcatgaacgc gggcctgtcg
     1981 ctgggccggc tcgaccagtg gctccagccc tatttcctcg ccgacatgca gcagctctct
     2041 tcccgggaag accgcgcaga atacattcgg gatgcgatcg aactcatcgg gcacttctac
     2101 atgcgctgca cggaccattt ccctctcacg ccggatctgg ccaactttta ttttggcggg
     2161 agctcttccg accaggccat cacgctgggc ggcgtcacgc ccgaaggctt agacgcggtc
     2221 aacgacatga cctacatctt tctgaaagtg accgaaatgt taagcctgcg cgatcccaac
     2281 gtgaacgcgc gctttacgcc cggcatcagc agcgatgctt atctgaaacg cctctgcgag
     2341 gtaaatctga tcaccgcggc aacgccgtcg atgcacaatg acaaggccat gttcgcggcc
     2401 ctgtctccca tggggtatga tattcgggat atccgcaact ggtcggcaac gggctgcgtc
     2461 gaacccacgc tttcgggcaa gcacatggcc cacaccaaca tgcagatgat gaacatggtc
     2521 gccgcccttg aaatggcgct caacaacggg ctccaccccc tcacggactg gaaactgggt
     2581 ccggaaaccg gcgcaatcga gcaaggcgac ttcaatagct ttgaggactt cttcaacgcc
     2641 tttaccgagc agtttgcctt tctcatcgac cgctccatcg aatacaacga tctgctggcc
     2701 ctggcccatc agtacctgcg ccccacgccg ctgctctctt ccgtgatcga cggatgcatc
     2761 acaaaaggaa aagacgtcac cagggggggg gcaacctaca acagttccgg aaccgccatt
     2821 gtcggccttg ccgatgtcac cgattccctg atgaccatca aaaagctggt ttttgatgaa
     2881 aagaagatct cgtttgcgca tttaaagaag gcggtggacg gcaactttga aaacgatccc
     2941 gaactgctgg ccatggtgcg caggaaagtt ccgcttttcg gctcggggaa tgcggaagcg
     3001 gtggagatgg ccaaccgcct gacccgctgg tcgcacgatc attatgcagg cctgacgcac
     3061 tacaggggcg gcaaatatac caccggtttc tggacgatgt cgcagcatgt ggcgttcggc
     3121 attctgacgg gcgcgctccc ttccggcaga caggcgggca aaccctttac gccgggtctc
     3181 acccctcagc ccttcgcttc gaaaaacctg ctcgacaaca ttcgggacgt tgcgaagctg
     3241 gatcccgtca acctgaacaa caacatcgcc ttcaatgtca aggtggtgcc gtcggccgcc
     3301 gacacacgtg aaaaaaccgt gcacaacatg ttctcctatg taaaaaccta tttcgatctg
     3361 ggcggcatgc agatgcagat gaacgtagtg acgtcggcca tgctgcgtga cgcaatggca
     3421 caccctgaaa actaccggaa cctcatcgtc cggatttccg gctataacgc ctactttgta
     3481 accctgaaca aagacatgca gcgtgagctg attgaacggg cggaatatgc aatgtgacgg
     3541 ggcggccatg gcccagacgg aagaatccca ggcccggaag cctctcattc ttgaaatcaa
     3601 ggggaactcg cttgacgacg gccccggcat tcgaaccgtc gtttttttta aaggatgccc
     3661 cctgtcctgt tcctggtgcc acaatcccga aagcaagctc gctcgggttg agatcggtta
     3721 tgatcgcaaa gagtgcatcg gttgcgggac ctgcacggcc gtctgtccgg aaaaagcggt
     3781 atcgccgcaa aatatccatc ctgtggatcg agaaaaatgc accctttgtt tccgctgtgt
     3841 tgaggaatgc cccgcgggcg ccctcgcccg cgtcggacga agcctgtcca ttgatgaaat
     3901 cgcggccgaa gtcttaaagg acaaaccctt tttcgacacc tccggaggcg gcgcaacctt
     3961 ttcggggggc gagccggtaa tgtttatgga gtttctgtcc gaattgctcc atgctttgaa
     4021 agcatatcaa gttcataccc tgctggaaac cagcggcttt tttgattttg aaaaattcga
     4081 aagattgctt cttccctgtc tggacatgat ttattacgac atcaaaatca tggatgagaa
     4141 agcgcatcgc attcacacgg gggtttccaa tcaacgaatt ctggaaaact ttgaaaggct
     4201 tttcaaaatc tccagggata ccgggatcac gcttctgccc cgaacgcccc ttgtgccggg
     4261 gatcaccgac cgccatgaga atattcaggg cattgcccga ttcctggccg agctgggcgt
     4321 caggaacgcg gcgctcatgc cgtacaatcc cctctggcat gaaaaggcgg ataaaatcgg
     4381 cgcggacaat ccccatcgcg gccatgccgt catggcggca tggatggaga aagaaaaggt
     4441 ggaggcctgc cggagaatat tttccgaaga aggcattcag gtcgtttaga ggccggatag
     4501 ataaagaagc tcccgcccgg catcggggat ttcggtcgaa aggttatctt caaactgctt
     4561 ctcccgtttc agggggagcc attcacccgg ttggccggac tttccatcgg gtcggcgcca
     4621 tggaaaaccg gcataaaaga aatcaggctt tccgtggata tggcggataa aatattcttt
     4681 acacaaacgg gagggttttg agtatacttg acttcattaa aagggaattt ttgtcaatga
     4741 cccttccggc ggtccatctc ccggcgagga acttccggaa aataattttt gaaggtgctc
     4801 atggaaatca agtttaaaat ctggctggaa aaagacggtc atgccctgtt cggcgaggga
     4861 cgcgaccagt tgctgaaagc catcgacgaa tgtcatggtc tttacggggc cgcaaaaaaa
     4921 ttgaaaatgt cctaccgcgc ggcctggggc aaaattaaat tgacggaaga gcgcatcggc
     4981 aagaaaatcg ttgaaacggg agaggacaag aagctgcacc tgacaaaaga cgccaaaaaa
     5041 attctggatg aatttgaaaa gctggaaaaa gatgtcgatg gttttctcca tgcgcgcagg
     5101 tccactttta tcgtgctgaa agatcccccc acgaaaagcg acgaaaagaa atgagtattg
     5161 aacgcatacc ccccccatcc tccggctatg ctcctccggg catatgctca cagatgcata
     5221 actatatgaa ataattgaaa ttattccttg acaatccctt aaaatccttt atgctaaatc
     5281 caacagaaca accggcgtct tcatgacgca ggatgtccgg acaagaaaat tttgagtgtc
     5341 ggacgatgtt cagagggact gaacgttcgg aagaccttca aagaatattt tgccggttac
     5401 atgtgtaaag gaggacattt ctatggaaga gaagttttca atgaaaagaa ggagtttttt
     5461 gaagctggcg ggagccgccg gtgttgccac cgcgatgacg gcttttccct tcaggaatat
     5521 gcgggcggca tgggccttcg gggatcatcc ccaggaacag ttgccgtatc gcattaccaa
     5581 aagagtcccg caggtttgcg caagagcctg cgaggcggac tgcgcttaca atgttgtggt
     5641 cggcgttgat cccgccaccg gtcttgaaag ggccctgacg ctggaaggac gtccggaaga
     5701 tcccgtgtcc aacggaaaat tctgcatcaa aggcatgggg tttgtggatt cgatgtacga
     5761 tccggaccgc ctgatggttt ctctgaagag gaccaacaag acgaagggaa ccgatgtgga
     5821 tcccggctgg attatcatga agacggaaga tgcggtcaac gaaatcatcg ccaggatgaa
     5881 aacctacaaa ccggaagaaa ttctgatggc gtcgccgggc gatccataca ccaaccgact
     5941 gtgtcagtcc atcggctgca cccggagcga tcagcggacg gaatgtttcg gcacacatta
     6001 ctacatcaac tgcctgacgc tcaccaatcc gcccaacaag ttttattcca gctgctacac
     6061 cccgagccat catgtgtgcg gttatgacta cgacacctgc aagtaccagg tctggttcgg
     6121 atttgactct ttttccaagt gtggcaaggc gggcatgctc aatcactggg cggcgggaaa
     6181 aagaaacggc ggcaagattg tcatgttcaa cccggtccgc acccccatga cggacaattt
     6241 tgccagtgag tattacgcga tcaaaccagg aacggatctg gccgttgcgc tggccatgat
     6301 taaccagatt atggccggca agaagtacaa caaggaattc atgaccaaat attcggacgg
     6361 cgcggctctg gttgatgtgg aaaaacagac gcatctgaaa acagccgacg gcatgtttct
     6421 ggcgtggtcc gtaaaaaaca aaaaagccga acccatcgat gaatgtgatg atccggcgct
     6481 tgacggctcc tataccgtaa cgcttgacgg acggcaaatc aaggccaaga cggttctgca
     6541 gcttctggcc gaggcgacaa agacctatac gccccagtgg gccgcgaaga tcagcgacgt
     6601 gccggccaag gccatcagca agatcgcccg cgattttgcc gccgccgcgc cctacgcgct
     6661 gattcccacg tacaagcgcg acgcggcggg ccccaattac gccaacagct ggcgtcttcg
     6721 ccacgccatc cagattctga actccctggc cggttcgatc gaccatgaag gcggcatgct
     6781 tctgctgcac gacgtcaaga ttccctggat cgacgaaatc tccccgccgg cgttgcctta
     6841 tcctgaacag ccgtcccagc cggtggactt ccgcaatgaa tttccggtca cggatgacat
     6901 ttaccggaag aaggattttt cggcgccggg tcattacggc atggtaggat gggggcttta
     6961 caagacaaac cgcgcgaagg ttgccttttt ccggaacccg caccgagggc tctttgccat
     7021 gatccattcc cagatgttcg aggccgcctc tcagaaactg gaactcgtcg tggactggaa
     7081 cctttatctg gacgatctgg gctacttctg cgactacgta gtaccggcgc ctcatcagtt
     7141 tgaagagggc aaaatggacc tgcgtctcta ctatccgaaa tatccctgca ttgtcggcgg
     7201 ttcgccggtg cagaaatcgc cgggagacca gatcggctgg ggcggtttcg ccatgaagat
     7261 cggtctggcg atggcgccga agtactggac gacggacggc agcggcaatc cggccaagaa
     7321 aatcccgaca aatatgggcg atgtcgcagt caagaaggcg ggcattgccg acagcgccgc
     7381 cgatttcatg aagaagggcg gtttgtggat cgataaaaaa gagtatcaga actaca
//
LOCUS       DAOSIM010000094         7435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_9128_length_7435_cov_7.403388, whole genome shotgun sequence.
ACCESSION   DAOSIM010000094 DAOSIM010000000
VERSION     DAOSIM010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7435
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9128_length_7435_cov_7.403388"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..902)
                     /locus_tag="PLR47_05385"
     CDS             complement(87..902)
                     /locus_tag="PLR47_05385"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOG12410.1"
                     /translation="MIVAGSYRRLGNCEMFAKDIALRARADSISLLRLTDFALKSCTG
                     CYRCLNPEHRCRVQDDLYFILEKMKGFDGIIFSIPTYVLGPVGQFKLFADRLACMAVF
                     QPDFRKIRAVSAIFGGIESWRGVTQSLVNAVIRMMGFDFRGSAFIEAALPGESLDVKY
                     QPVAQGLADALGENSAAYFPCKTLQCPSCGSDLFFIQDGRKVCVLCESSGEYRDGQWI
                     DYRDSKRFTARGFVEHFEGWLKPKVAEYSAQRERHRSLRETYKDIGVWIKKEE"
     gene            complement(942..1781)
                     /locus_tag="PLR47_05390"
     CDS             complement(942..1781)
                     /locus_tag="PLR47_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12411.1"
                     /translation="MSSKRAAFFFLSALLLCSAGCAAVFSQNDRYAAADRVADRAGFE
                     KSFIRTSSLTLTAFTRFTKKGDPLHVYIEGDGTAWLSRTRLSDDPTPRTPLVLELAAL
                     DSASNVAYLARPGQYAAEGVPDCDAAYWSEKRFAQEVVEAMNGALDELRRRAGAGAIH
                     LVGYSGGAAVAVLAAARRSDVAGLRTVAGNLDPEAVNRYHHVSAMKDSLNPMDAAEKV
                     RDLPQRHFVGSQDTVIPPGLARVFLKRVGCRDSSGITVVEGATHTRGWRERWRELLII
                     PLN"
     gene            complement(1771..4656)
                     /locus_tag="PLR47_05395"
     CDS             complement(1771..4656)
                     /locus_tag="PLR47_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HOG12412.1"
                     /translation="MKKHLCGLFWILLIALCLVEPAAAEMETFAQSVANAHANAAEPQ
                     GTLGRWIPTADSMYKVSTAGSVGANPYGLKYYGDPAQNQLIVRTASSENAYNGVYSGG
                     TITTAGSNYTIFGNKDVMSAWVTTGKEMTGFINSQGLTAGTFVKGVERGLGMNDRGNH
                     DAIFEMAVTVSNTANPYMLRPTRNPDPTQFSTDPATHGTNASFPATAQAAGIGTGAAA
                     DAVFANYKTAYTDWMTANYPWTQLGYTYYWGRAANKPENLSEVQGMSEFILLGGAGGS
                     DTAKTPSGTDESGNVVVVGIYASQSYIYTKNDGTTFSDAPDAQYGNGFASFNVTGPCD
                     TLWAGRDFQVGASLNPAALPNTITIAADGSIAGGQGILVGSQNYTVTNAGSITANAGA
                     KKFNVAGSENIALLFKGDTHAAPGTLKNTLINSGTITAPGANGTAVAAWAGDTLIVNT
                     GTIIGTGTGYAIKTGAGNDTLTVNGGEIAGRVDLGTGTDNVNVPAGSSARFTFSLNTD
                     APAAAQIKSQTVAIADNTTTVAVRTTGTTNIKNNDQFLIADATTLTVDPAKLSVINDP
                     AFPMFTFSPQTSGSRLFLTALRDAAFYGQRVGNASLGYVLDSLANTATGDMAFVLGAL
                     DSSGDPAGAGQMQPTVNNGPIQASFGTVSQYTSTVLNRIEQVAAARTDGIGMTGISTG
                     DETAQGGVWAQGFGSYLHQGAQQASQGYTANVWGISFGYDRYLFQNIIAGFSGAFARN
                     NVTTGDVNTRTDADSYQGSLYGSLSKDAWYLDAILSFAYNRYDASRHIVFAGIDRTAK
                     SDYGGYQYSGYFEGGYTVKASGLLLTPLVSVQAMRLNLDDYTETQAGALNLKIDRQRY
                     NLFQTGLGAKVAYPVSKKNLKITPELHAKWLYDFAGDAQQAASTFTGGGASFVTTGFE
                     PPRTSGNIGAKLTILTRTNWSVSFNYDFEMKQDFYSHNGWVNLRYEF"
     gene            4877..6292
                     /locus_tag="PLR47_05400"
     CDS             4877..6292
                     /locus_tag="PLR47_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOG12413.1"
                     /translation="MSLSEELKYFDATELAGLIRKKEITPLELVESSIERIEKLNPAL
                     NAVITPMYDLARDAAASKLPDGPFTGVPFLLKDILASYGGVRMTFGAKVLKDLVPDHD
                     SELVARHKKAGLIVIGKTNVPEFGILPTTEPELFGPARNPWNTNCSTGGSSGGSAAAV
                     AARIVPMAHGNDGGGSIRIPASCCGVFGLKPTRGRITLGPDFGDMIGGLVNEHALTIS
                     VRDSAALLDATAGCLRGDPYWAPPVERPFVKEANTDPGRLRIAFAAKAPTDDPVHEDC
                     VRAVQDAARLCEELGHSVEEAAPVIDGKTLTHAFVVPWFAGIASIVKMLGVTKTQVEP
                     LTWAIYERSEASTAADYLIAVQTIQRVARDVASFFEQYDVLLTPTLTKPPAPLGSFEP
                     TPEDPLAGFAETARYAVFTPICNATGQPAMSVPLFWNSEGLPVGAHFIGRFGDEATLF
                     RLAGQLEKARPWKDRRPPVCA"
     gene            6305..6793
                     /locus_tag="PLR47_05405"
     CDS             6305..6793
                     /locus_tag="PLR47_05405"
                     /inference="COORDINATES: protein motif:HMM:NF001095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HOG12414.1"
                     /translation="MQIFVDADAFPNGIRDILIKASLRLNIPLIFVANKPLHLEKLPN
                     LSLILVPEGPDVADDRIAQSVRPGDLVITADIPLADRVVDKNAFAMNPRGMLYTEHNI
                     KDRLAMRDLLGELRDNGMITGGPAAFSKKDRQAFANQLDSFLVRLMKPVKVMHQKPVS
                     DS"
     gene            6991..>7435
                     /locus_tag="PLR47_05410"
     CDS             6991..>7435
                     /locus_tag="PLR47_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG12415.1"
                     /translation="MAQKKIIKKFIALMMILAFMLFSIGVWAAENPAAQKPDYDVIVI
                     GAGMGGLSAAAHLAVKGQKVLVLEKHFVVGGSTTKFTRGEFTFDAALHEMAGGGPGKM
                     DRGLYKLLKATGVDKKVELYEVPHFYRSIFPGVDITLPPNWPGFKS"
BASE COUNT         1572 a         2128 c         2138 g         1597 t
ORIGIN      
        1 cccttcgacg ggctcagggt gacaaatgtg actttttacg agtttgtcaa ctttgagcgg
       61 gccgctgttt ttttgcggcc cgttttctac tcttcttttt taatccagac gccgatatct
      121 ttgtaagttt cccgaagcga gcggtggcgc tctctttggg cgctgtattc cgcaaccttg
      181 ggtttgagcc agccttcaaa gtgctcgaca aaacccctgg cggtaaaccg tttggaatcc
      241 cgatagtcaa tccattggcc gtcccggtat tcgcctgagc tttcgcacag gacgcaaacc
      301 tttcgcccgt cctgaataaa aaaaagatcg ctgccgcagg aagggcattg cagggttttg
      361 cagggaaagt aggcggcgga attttccccc agggcgtcgg ccagcccctg cgccacgggc
      421 tggtatttca catcaagact ttcgccaggc aacgcggctt caataaaggc gcttccccga
      481 aagtcgaagc ccatcatgcg aatcacggcg ttgaccagag actgcgttac tccccgccag
      541 ctttcaatgc cgccgaaaat cgcgctgacc gcccgaattt tccggaagtc gggctggaac
      601 acggccatgc aggccaggcg gtcggcaaac agcttgaact gcccgaccgg ccccaataca
      661 taggtcggta tggaaaagat gattccgtcg aaccctttca ttttttcaag aataaagtac
      721 agatcgtcct ggacgcggca gcggtgctcc ggattgaggc agcggtaaca gcccgtgcag
      781 gatttcaagg caaagtccgt aagacgaagc aaactaatgg aatcggcgcg ggcgcgaagg
      841 gcgatatctt ttgcaaacat ttcacagttt cccagacgcc ggtatgaacc tgcaacaatc
      901 aaaagcgaag tccccaaatc tgccctcctg atatctaatg ctcaattcaa aggaatgatc
      961 agtaattctc tccagcgctc ccgccatccg cgggtgtggg ttgcgccttc gacaacggtt
     1021 atcccgctcg aatcccggca gccgacacgt tttaaaaaaa cccgggcaag cccgggcgga
     1081 atgaccgtgt cctgcgaacc cacgaaatgg cgctggggaa gatcccggac cttttcggct
     1141 gcgtccatcg ggtttaaaga atccttcatg gcactgacat gatggtaacg attcaccgct
     1201 tccggatcaa gatttccggc cacggtcctc aggccggcca cgtcacttcg ccttgcggca
     1261 gccagaaccg cgacggccgc tccgccggaa taaccgacca gatggatcgc tccggcgccg
     1321 gcccgtcggc gcagttcgtc cagagccccg ttcattgctt ccaccacctc ttgcgcgaaa
     1381 cgcttctccg accagtaagc cgcatcgcaa tccgggacgc cttccgcggc atattgcccg
     1441 ggacgcgcca gataagcgac gtttgacgct gaatccagcg ccgccagttc cagaaccaga
     1501 ggggttcggg gagtgggatc gtccgaaagc cgggtgcgcg aaagccacgc cgtcccgtct
     1561 ccttcgatgt aaacatgcag gggatcaccc ttctttgtga atctcgtaaa ggcagtcagt
     1621 gttaacgatg aagttcgaat gaaggatttc tcaaatcccg cgcggtcagc cacacgatcg
     1681 gctgccgcgt accggtcgtt ttgcgaaaaa acagccgcgc agcccgccga acacaaaagc
     1741 aaggcggaca gaaagaagaa ggcggctctt ttagaactca taacgcaagt tgacccagcc
     1801 gttgtggctg taaaaatcct gcttcatttc aaaatcgtag ttgaatgaaa cgctccagtt
     1861 ggtccgggtc agaatggtca gcttcgcgcc gatgttgccg ctggtccgcg gcggctcaaa
     1921 accggtcgtg acaaaggaag cgcctccgcc ggtgaacgtt gaggcggcct gctgcgcgtc
     1981 gcccgcaaag tcatagagcc acttggcatg cagctccggc gtgattttca gattcttttt
     2041 cgaaaccggg taggcgactt tcgcgcccag gcccgtctgg aagaggttgt agcgctggcg
     2101 gtctattttc agattcagag cgccggcttg tgtttccgta tagtcatcca gattcaggcg
     2161 catcgcctgc acggagacca gcggcgtcag gagaagcccg gaggctttga ccgtgtatcc
     2221 gccttcgaaa tagccggagt attgataccc gccgtaatcg ctctttgccg tccggtcgat
     2281 tccggcaaaa acgatatgcc gggaggcgtc ataccggttg taggcaaacg acaggatggc
     2341 gtcaagatac caggcgtctt tggacagact gccgtaaagg ctgccctggt agctgtcggc
     2401 gtcggtgcgg gtgttcacgt cgcccgtcgt aacgttgttt cgggcaaacg cgccgctgaa
     2461 gcccgcgatg atgttttgaa aaagatatct gtcatacccg aaggaaattc cccagacatt
     2521 cgccgtgtag ccttggcttg cctgctgagc tccctggtgc aggtagctgc cgaatccctg
     2581 cgcccagacg ccgccttggg cggtttcatc cccggtggag atgccggtca tgccgatccc
     2641 gtccgtccgg gccgctgcaa cttgttcgat gcggttcaga acggtgctgg tgtattggct
     2701 gactgtcccg aaactggcct gaatcggacc gttgttgacg gtgggctgca tttgcccagc
     2761 gcctgccgga tcgccggatg aatccagtgc gccaaggaca aaagccatat cccccgtggc
     2821 ggtgttcgcc aggctgtcca ggacataacc cagcgaggcg ttgccgactc tttgtccata
     2881 gaaggcggcg tcgcgaagcg ccgtaaggaa aagccggctg ccgcttgttt ggggagaaaa
     2941 agtgaacatg ggaaacgcag ggtcattaat gacggatagt tttgccggat caaccgtcaa
     3001 cgtggtcgca tccgcaatca aaaactgatc gttgttttta atgttggttg tcccggtggt
     3061 tcttacggct accgttgtcg tgttgtcggc aatagcaacg gtttgagatt tgatctgtgc
     3121 ggcagccggc gcatcggtat ttaaggagaa agtaaatctg gcgctggaac ctgcgggtac
     3181 gttcacattg tccgtccccg tccccagatc gaccctgccg gctatctctc cgccgttgac
     3241 cgtcaatgtg tcatttcccg cgccggtttt tatggcgtaa ccggttccgg ttccgatgat
     3301 cgttccggtg ttgacgatga gagtatcgcc ggcccaggcg gcgacggccg tgccgttggc
     3361 gccgggagcc gtaatcgtgc cggaattgat caacgtgttt ttcaaggtgc ctggtgccgc
     3421 atgggtatcg cccttgaaca gaagcgcgat gttttcactt ccggcgacgt taaacttctt
     3481 tgcgcccgca ttggcggtga tcgatccggc gtttgtgacc gtatagtttt gggacccgac
     3541 caggatgccc tgcccgccgg caatcgatcc gtcggcggcg atggtaatcg tgttgggcaa
     3601 ggcggcggga ttcaaacttg cgccgacttg aaaatccctt ccggcccaga gcgtgtcgca
     3661 ggggccggtg acattgaagc tggcaaatcc gtttccgtat tgagcgtccg gcgcatcgct
     3721 gaaggtcgtt ccgtcgtttt tcgtatagat ataggactgt gacgcgtaga tgccgacgac
     3781 gaccacattt cccgactcat ccgtgccgga aggggttttg gccgtatcgg agccgcccgc
     3841 gccgcccagc aggatgaact cggacattcc ctgcacttcg gaaaggttct cgggcttatt
     3901 ggccgcccgg ccccaataat aggtatagcc caactgcgtc cagggatagt ttgccgtcat
     3961 ccagtcggtg taggccgtct tgtagttggc gaacaccgca tccgcagccg cgccggttcc
     4021 gatgcccgcc gcctgggcgg tagcggggaa agaagcattc gttccgtgtg tggcgggatc
     4081 cgtgctgaat tgcgtcgggt cgggattccg tgtcggccgc agcatatagg gattagccgt
     4141 gttgctgacg gttacggcca tttcgaagat ggcgtcatga ttccccctgt cgttcatgcc
     4201 aagtccccgt tcgactcctt tgacaaacgt gccggcggtc agaccctggc tgttgatgaa
     4261 tcccgtcatc tcctttcccg ttgtaaccca ggcgctcatg acgtccttgt tgccgaaaat
     4321 ggtgtagttg gatccggcag tcgttattgt tcccccggaa tacacaccgt tataggcatt
     4381 ttctgaactg gccgtgcgga caatcaactg attttgagcc ggatcaccgt aatatttcag
     4441 gccgtaggga ttggccccga ccgagccggc ggtggaaacc ttgtacatgc tgtccgccgt
     4501 cgggatccat ctccccagcg tcccctgcgg ctcggcggcg ttcgcgtggg cgtttgccac
     4561 cgattgggcg aaggtttcca tctccgccgc agcgggctcg accaggcaaa gagcaatcag
     4621 aagaatccag aataaaccgc aaagatgttt tttcatattt acctctcagc ctcgaaaaaa
     4681 gatgcaaccg gccgctctga aaaagaatga aatccaagcg tggttgtttg cgcgggagta
     4741 taaaaaacac cccggccgct gtcaagcgaa tacaagggcc aaatactaca agagccaaat
     4801 actgcttgac ggttaaaaac ggtcccttta tacttcgtgg cgtcaaaggc aaaatatcac
     4861 cgggtaagga ggtcgtatgt cgctgtcgga ggaactgaaa tattttgatg ccacagaact
     4921 ggccggtctg atccggaaaa aagagatcac gccgcttgag cttgtcgaat cttcgattga
     4981 acggatcgag aagctgaatc cggcgctcaa cgcggtgatc acgccgatgt acgatctggc
     5041 gcgggacgcc gcagcatcaa agctgcccga cggccccttt accggcgtgc cgtttcttct
     5101 caaggatatt ctggcgtcct acggcggcgt gcgcatgacg ttcggcgcca aagttttgaa
     5161 agaccttgtc ccggatcatg acagcgaact ggtcgcccgc cacaaaaagg ctggcctgat
     5221 tgtgatcggc aaaaccaacg tcccggaatt cggcatcctt ccgaccactg agccggaact
     5281 cttcggccct gcccgaaatc catggaacac caactgctca accggcggct caagcggcgg
     5341 ctcggccgcg gcggttgcgg cgcgcattgt ccccatggcg cacggcaacg acggcggcgg
     5401 atcgattcga attcccgcct cctgctgcgg cgttttcggc ctgaagccga cccggggcag
     5461 aatcaccctg gggcccgatt tcggcgacat gatcggcgga ctggtcaatg aacatgcctt
     5521 gacgatttcc gtccgggaca gcgcggctct gctggacgcg acggcgggct gcctgagggg
     5581 cgatccctac tgggcgccgc ccgtggaacg gccgtttgtg aaggaagcga atacggatcc
     5641 gggacgcctg cgcatcgcct ttgccgcaaa agcccccacc gacgacccgg tgcatgaaga
     5701 ttgcgtccgc gcggttcagg atgcggcccg gctgtgcgag gagcttggcc attcggttga
     5761 ggaggccgcc cccgtcatcg acgggaaaac cctgacccac gcctttgtcg tcccgtggtt
     5821 cgccggcatc gcatccatcg tcaagatgct tggggtgacg aaaacacagg tcgaacccct
     5881 gacctgggca atttacgaac gcagcgaagc ttcaaccgca gccgactacc tgatcgccgt
     5941 acagaccatc cagcgggtcg cccgcgatgt cgcctcgttc tttgaacagt atgacgtttt
     6001 gctgaccccg acgctgacaa aaccgccggc gccgctgggc tcgtttgaac ccactccgga
     6061 agatccgctc gcgggatttg ccgagacggc ccgctacgcc gttttcacgc cgatctgcaa
     6121 cgcgaccggc cagccggcca tgtcggtgcc gcttttctgg aacagcgaag gactgccggt
     6181 gggcgcccat ttcatcggcc ggttcggcga cgaagccacg ctgtttcggc tagccggcca
     6241 gctcgaaaag gccagaccat ggaaagacag gcgtccaccc gtttgcgcat aggattaaga
     6301 ttccatgcag atattcgtcg acgcggatgc tttcccgaac ggaatcagag acattctgat
     6361 taaagcatct ttacgcttga atattccgct gatctttgtg gccaataaac cgttgcacct
     6421 ggagaaactg cccaaccttt ctttgatcct ggttcccgaa ggaccggatg tggccgatga
     6481 ccgcatcgcc caatccgtgc gcccgggcga tcttgtcatc acggccgaca tcccgcttgc
     6541 cgatcgcgtg gtggacaaaa atgcttttgc gatgaatccc cgcggcatgc tctacacgga
     6601 gcataacatc aaagaccggc tggccatgcg ggatttgctg ggtgagttgc gggacaacgg
     6661 catgattacc ggcggcccgg cggccttcag caagaaagac cgccaggcct tcgccaatca
     6721 gcttgatagc tttttagtcc ggctaatgaa acccgtaaaa gtgatgcacc aaaaaccggt
     6781 atccgactca taacacaaac acgaatcatc acgtccggta gtacgagtct ttagatccgc
     6841 tgttttggat ggtggatggt agatggtgga tagtgaatag aagggcaaag cgcacccaga
     6901 tgtcgaaatg gcaatttgaa caccaggcaa acttgacaag cggtcgcttt aatgggatga
     6961 ttcgcacaaa tttttcccgg gaggtttctg atggctcaga aaaaaataat caaaaaattc
     7021 atagcgttga tgatgattct ggcgttcatg ttgttcagca tcggcgtatg ggcggcggaa
     7081 aatccggcgg cgcagaaacc cgactatgac gttatcgtca tcggggccgg catgggaggg
     7141 ctttccgcgg cggcgcacct ggccgtcaag gggcaaaaag tgctggttct ggaaaagcac
     7201 tttgtggtgg gcggcagcac aaccaaattt acacgcggag aatttacctt tgacgcggcg
     7261 ctgcatgaaa tggccggcgg cggcccgggc aagatggaca gggggctgta taaactgctc
     7321 aaagctaccg gtgttgacaa aaaagtggag ctctatgaag tcccgcattt ttaccgatcc
     7381 attttccccg gcgtggacat cacgcttccg ccgaactggc cggggttcaa aagcg
//
LOCUS       DAOSIM010000095         7431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_9133_length_7431_cov_8.764778, whole genome shotgun sequence.
ACCESSION   DAOSIM010000095 DAOSIM010000000
VERSION     DAOSIM010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9133_length_7431_cov_8.764778"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1384
                     /locus_tag="PLR47_05415"
     CDS             179..1384
                     /locus_tag="PLR47_05415"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOG12416.1"
                     /translation="MAGAELIGQEEIKEVMDVLETGILMRYNFDQERKFVFKVRQFEE
                     EFAKYCGVKYALGVTSGSAALKIALEAMDVGPGDDVLVPAFTFLATYEAVLEVGAIPI
                     MVDIDDTLNLDPDEIEKKMTRYTKAVIPVHMCGLAANIDKIIKIARKNKLMVLEDNAQ
                     GCGASFKGKKLGSFGDMGTFSFDYVKTVTTGEGGMVITNKKNLYLRSEWYHDHGHDHN
                     PRVGRALEGRTILGFNYRMNELQGALGLAQLRKLDYLIGEQKKHKKMIMDVLAAALPN
                     VGFRAKRDPEGDSATFLAFNLPEEKLALKFQKLLSAEGLATTCYKNNKWHYVPNWEHF
                     LAFSTANSKKYPFKSKEYKGKVKYDRKSIPFAEDILGRTLVMGISVKMSPERLDTMKK
                     AIENAAKNM"
     gene            complement(1510..2103)
                     /locus_tag="PLR47_05420"
     CDS             complement(1510..2103)
                     /locus_tag="PLR47_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOG12417.1"
                     /translation="MEQEDLIVDVRTREEFLKEHVKGAINIPLYDVSFYTGILRGRKI
                     RAYCNTGHRAALALEKMKALGVDAAMIEPKDLDKMEKERVNILCAVNFVSARPGGEEK
                     FLQEMIGICRATEGMDGYLGSKVLEVSGVSAAGSFLSESCGDLEIIPCKYIILTYWTS
                     KEAHEKSHQIPEFLDRYAAIPAHLTQVPYEEFYEILK"
     gene            complement(2203..2700)
                     /locus_tag="PLR47_05425"
     CDS             complement(2203..2700)
                     /locus_tag="PLR47_05425"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HOG12418.1"
                     /translation="MTRIKNRPKHTNKLLNAVRGLLEHRATWLYLLLDEAEKRGVETE
                     EFARSAIMRCGCFQGKHLADAAGTQSLRGLRKTLFTLPARMVFEMKILACDDDRLDID
                     FHYCPLVAAWREQGASDEKIAVLCDIAMQGDRGIARSFGCELVLGETIAKGHDKCEVR
                     FSRTG"
     gene            complement(2806..3195)
                     /locus_tag="PLR47_05430"
     CDS             complement(2806..3195)
                     /locus_tag="PLR47_05430"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HOG12419.1"
                     /translation="MSIRIVRLGTNRSPNEGLRVGTVRRPPRGVPKSEYAVQNWYDVW
                     FPNLSPSPETIKLGQQAKNPNDWKAFFRKFRAEMTTPENSKALDLLAALSHQTNFSIG
                     CYCAEEERCHRSALRALLLERGAAVER"
     gene            3354..4190
                     /locus_tag="PLR47_05435"
     CDS             3354..4190
                     /locus_tag="PLR47_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12420.1"
                     /translation="MVKRNGITGWLFAQAMILLLLWPATVYCAAGEDQVLEAARQKVA
                     AELSRLDAGLREVAAELGKTGLAGKSARPALAKLCGKFDYAVDCAAVNLQGRMATIEP
                     EAYRRFEGKDISGQEQVRRVFKTGRPALSSVFVSVEGFPAVDAEYPVVSSGGKRIGSV
                     SVLFRPEMFLGSVFVPLTRQTSVEIWAMERGGLILHDADASQIGLNLFTSKRYQPYAG
                     LLALGKRIAARPQGKGVYEFTQGQSANVVRKNALWKTVSLYGTSWRLVAVTVEHPAGK
                     AK"
     gene            complement(4298..5242)
                     /locus_tag="PLR47_05440"
     CDS             complement(4298..5242)
                     /locus_tag="PLR47_05440"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOG12421.1"
                     /translation="MKKRIIPVGLLILLMIIACAPANSSRQRDGSNESQETRLTVSDE
                     KRLTEEALPKMRQDYPPANNPELQRYIADLGGKIVRANKLDGNPYRYNFTVVDVPDVN
                     AFAMPAGTVFVTAPLIAAASNEAELAGVVSHEIGHVVARHTAERMYAMEKAQNKTWLY
                     TAGGAVVGAAVGYGAGRLLCKDGDTSCHAQAAAIGGALGAGGGLLAQKYAFLVNSRED
                     EMEADRIGFKYSVAAGYDKNEVGKFYEKLLQMEKSRGADDSGVMKSLSDAFSTHPPSE
                     ERVQQMKELAETSPAKKGIVNTASFNRAKQIAAGLKKK"
     gene            5544..6569
                     /locus_tag="PLR47_05445"
     CDS             5544..6569
                     /locus_tag="PLR47_05445"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HOG12422.1"
                     /translation="MKKTILPIVLFCLAAWFPLPAAHADEITLENGSAIIGTITRIDQ
                     KAILVRTDFAGVLSVDMTRVLTYTTDHPVYVAFPGHDPVRGTLTYTRKQTRIDAADGK
                     VVVSGDAPEHVWPEGATDPRARHWGFEVGLDAGGKTGNAERLSVGSRAQAKLQGPSDR
                     LLLYLRYAYAKDDGIESANQTAGGIDFEAYFREKHSWYARVEAENDQIAAVDFRATAA
                     AGYGCYFLKKTHHTLRGRTGLMFRHETLKHESSRATFGLDLGISHLYRFANAWRLTND
                     VTYTPSLQDYLDYRIYHESAFDIPVVASEVWKLRLGVSNDYNSKPAENKVYLDTTYFT
                     RLIFSWR"
     gene            6650..>7431
                     /locus_tag="PLR47_05450"
     CDS             6650..>7431
                     /locus_tag="PLR47_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOG12423.1"
                     /translation="MSRRTGIVKDGRYLQHATGMDHPESPERLAAVYEMLDNPHMSWK
                     YESIEPREASHEEIAYVHTLPYIQEIAGTAGKDCVFLDSDTIAGPESYEIAKLAAGGM
                     INAIDAVITGEVDNAFAFVRPPGHHAGAGNSEGFCIFNNVAIGAMHAMKKHGLKKILI
                     VDWDLHHGNGTQKMFYSDRRVLYFSTHQYPYYPGTGSLMEIGEGQALGYTVNVPLRRG
                     AVNGTFVSAFRRILEPIANAFAPELILVSAGYDIYYQDPLGG"
BASE COUNT         1610 a         2037 c         2119 g         1665 t
ORIGIN      
        1 ggcggatgtg gcgatgacgg tggcccggcc gctggagaac aatccccgca agatcaccaa
       61 agaagacatg atcgcgattt acgcggaagc attctaaaat tcaaataagc aaagatacgg
      121 tacgaacacg cagggaacgg tcgcgaccgt tccctgcaaa gacatcgagg aggaatttat
      181 ggcaggggcc gaactgatcg gacaggaaga aatcaaggaa gtcatggacg tgctggaaac
      241 cggcattctg atgcgttata attttgatca ggaaagaaaa ttcgttttca aagtccgcca
      301 gtttgaagaa gagtttgcca aatactgcgg cgtcaaatac gcgctgggcg tgacctccgg
      361 ttccgcggcg ctcaaaatcg ccctggaggc gatggacgtt ggtcccggcg acgatgttct
      421 ggttccggct tttacgtttc tggctactta cgaagcggtg ctggaagtcg gcgcgattcc
      481 catcatggtg gatattgacg acacgctgaa ccttgatccc gatgaaattg aaaagaagat
      541 gaccaggtac accaaagcgg tcattcccgt ccatatgtgc ggtttggcgg ccaacatcga
      601 caagatcatc aagattgccc gcaagaacaa attgatggtg ctggaagaca acgctcaggg
      661 ttgcggcgca agcttcaaag gcaaaaaact gggcagcttt ggcgatatgg gaaccttcag
      721 ctttgattat gtgaaaaccg taacaaccgg tgaaggcgga atggtgatca ccaacaagaa
      781 aaatttgtat ctgcggtcgg aatggtatca tgatcacggc cacgatcaca atcccagggt
      841 cggccgggcg ctggaaggca gaaccattct ggggttcaac taccggatga atgaactgca
      901 gggcgcattg ggtctggcac agttgagaaa actggattac ctcatcggcg agcagaagaa
      961 acataaaaag atgattatgg acgtgctggc tgccgccttg ccgaatgtcg ggttccgcgc
     1021 caaacgcgat ccggaaggcg attccgcaac cttcctggcg tttaatctgc ctgaagaaaa
     1081 actggcgctc aaatttcaga aactgctttc ggccgaaggc cttgcgacga catgctacaa
     1141 gaataacaaa tggcattatg tgcccaactg ggaacacttc ctggcatttt ccacggctaa
     1201 ctccaaaaaa tatccgttca aaagcaagga atacaagggc aaggtcaagt atgaccgcaa
     1261 gagcatccct tttgcggaag acattttagg ccgcacgctg gtgatgggga tttcggtgaa
     1321 aatgagcccc gaaagactgg atacgatgaa gaaggcgatc gaaaacgccg cgaagaatat
     1381 gtaagattca gcgcgggcgg gccccaggaa aggcccgccc gcgcccgtaa tcgacagaat
     1441 acgccccgtg ggaattgcgc atgaatcccg ttttgtcttt cacagatctt gcccgtcgga
     1501 aaatggcctc tacttcagaa tctcgtaaaa ctcctcgtag ggcacttgcg tcagatgcgc
     1561 gggaatggcg gcataacgat ccagaaattc cgggatttga tgagacttct cgtgcgcttc
     1621 cttggatgtc cagtaggtaa ggatgatgta tttgcaggga ataatctcga gatcgccgca
     1681 gctttccgag aggaagcttc ccgccgccga cacaccggac acctccagca cctttgaccc
     1741 cagatatccg tccatgcctt ccgtggcccg gcagatcccg atcatttcct gcagaaattt
     1801 ttcctccccg ccgggtctgg ccgaaacaaa gttcacggcg caaaggatgt tgacgcgctc
     1861 cttttccatc ttgtcgagat ctttcggttc gatcatcgcc gcgtccacgc ccagcgcctt
     1921 cattttttcc agggccaggg ccgcccggtg gccggtgttg cagtacgccc gaatttttct
     1981 gccgcgcaga atgccggtgt agaaggagac gtcgtaaagg ggaatgttga tggcgccttt
     2041 cacatgctct ttgagaaatt cttcccgcgt ccgcacatcc acaatcagat cttcttgttc
     2101 cattccggtt tatctccctt attttaaaag tggtctgtcc gggagaagaa aggatttttt
     2161 gcttctcccg tcctaaaatg ttgccggggc actcccggtg aatcaaccgg ttctgctgaa
     2221 ccggacctcg cacttgtcat ggcctttggc gatggtctct cccaggacca gttcgcagcc
     2281 gaaggaacgg gcaatgcccc ggtcgccctg catggcgatg tcgcaaagca cggcgatttt
     2341 ttcgtcggag gctccctgct cccgccaggc ggcaaccagc gggcaataat gaaaatcgat
     2401 gtccagccgg tcgtcgtcgc aggccagaat tttcatttca aacaccattc tggccggcag
     2461 ggtgaaaagg gttttcctga gccccctgag gctttgggtg cccgcggcat cagccaggtg
     2521 cttcccctga aagcacccgc atctcataat ggccgacctc gcgaattctt ccgtttcgac
     2581 gccccgcttc tccgcttcat ccagcagaag ataaagccag gttgctctgt gttccagcag
     2641 gcctctgacc gcgttgagca gtttgtttgt gtgcttcggg cggtttttga tgcgggtcat
     2701 gctttcctcc tttgtcctat tttgcaacgc accaggcgtt tcctgaagaa tggcttttcg
     2761 tgttcacgcg aagccacacc atggtgacgg ggacttaaat cgacgtcaac gttccactgc
     2821 ggcgccccgc tcgagaagaa gtgcgcgcag cgcggaccgg tggcagcgtt cttcttcggc
     2881 gcagtagcag ccgatggaaa aattcgtttg atgcgacagg gcggccagca ggtccagggc
     2941 tttgctgttt tccggcgttg tcatttccgc tctgaatttt cggaaaaacg ccttccagtc
     3001 attggggttt tttgcctgtt gtcccagctt tatggtttcc gggctggggg ataggttggg
     3061 aaaccagaca tcataccagt tttgtacggc gtactcgctt ttgggaacgc cgcgcggagg
     3121 ccttcggacc gttccgacgc gaagaccttc gtttggactt ctgtttgttc ccaggcgaac
     3181 gatgcgaatg ctcatattgc ctccctgcgg ttgaaaaacg tccggcgctt gcacaatccc
     3241 ggttgattct tatagggttt tcacggataa ttgtcaacaa cggctccgtg gatgaaccac
     3301 ggcggcggtc attcagcaag acagtttcca tcaacgacgg gaggagaagg gaaatggtga
     3361 aaagaaacgg aataacggga tggctgtttg cgcaggcgat gattctgctg ttgctttggc
     3421 ccgcaactgt ttattgtgcg gccggagagg atcaggtgct ggaggcggcc cggcagaagg
     3481 ttgccgcgga attgagccgt ctggacgcag gtcttcgcga ggtggccgcg gaactgggga
     3541 aaaccggcct tgccggaaag agcgcaaggc ctgctcttgc caagctttgc ggaaagttcg
     3601 actatgcggt ggactgcgcg gccgtaaatc ttcagggccg catggcgacg atcgaacccg
     3661 aagcctatcg gcggttcgag gggaaagaca tttccggcca ggaacaggtc cggcgggttt
     3721 ttaaaactgg ccggcccgca ttgagcagcg tctttgtttc tgtagaagga tttccggcgg
     3781 tggacgccga atacccggtc gtgagctccg gggggaaaag gatcggttcc gtgagcgttc
     3841 tttttcggcc ggagatgttt ctcggcagcg tttttgttcc tctgacgcgg caaacctccg
     3901 tcgagatatg ggccatggaa agaggcggcc tgattctgca tgacgcggat gcgtcgcaga
     3961 tcggattgaa cctgtttacc tccaaacgct accagcctta cgccggtctt ctggcgctgg
     4021 gaaaacgaat tgccgcgcgg ccgcagggaa aaggagttta cgaattcacg caaggtcaat
     4081 cggcaaacgt tgtccggaag aacgccttgt ggaaaaccgt atctctatat ggtacgtcgt
     4141 ggcgcctggt ggccgtcacg gtggagcatc cggctggaaa agcgaagtag gaatcctgtc
     4201 ttcttcaaac aggaactttg cagcacgccc tgatcttcag ggcaggcaaa ggccggcagc
     4261 cgggctgttg cgggcgcccc cgttgtcgcg tcatatgcta ttttttcttg agccccgcgg
     4321 caatctgttt tgcccggtta aacgacgcgg tattgacgat gcccttttta gcggggcttg
     4381 tctcggccag ttccttcatc tgctgcaccc gctcctcgct gggaggatgg gtggaaaacg
     4441 cgtcggagag gcttttcatc acgccgctgt cgtccgcgcc gcggcttttt tccatttgca
     4501 gaagtttttc gtaaaatttc cccacttcat tcttgtcgta tccggcggcg acggaatatt
     4561 taaacccgat gcggtccgcc tccatttcgt cctcgcgtga attgacgagg aaggcgtact
     4621 tctgcgccag cagcccacct cccgcgccga gtgcgcctcc gatggccgcc gcctgggcgt
     4681 ggcaggacgt gtcgccatcc ttgcagagca gccttcccgc tccgtagccg accgccgcgc
     4741 cgacgacggc accgcccgcc gtgtaaagcc aggttttgtt ctgggctttt tccatggcgt
     4801 acatccgttc ggccgtgtgg cgcgcgacga cgtggccgat ttcatgggag acgacgccgg
     4861 ccagctccgc ttcattggag gcggcggcga tcagcggtgc ggtgacaaag acagttccgg
     4921 cgggcatggc gaaagcgttg acgtccggca cgtccacaac cgtaaaattg taacggtatg
     4981 gatttccgtc cagtttgttc gcccggacga ttttcccgcc caggtcggct atatagcgct
     5041 gaagctccgg attattcgcc ggaggataat cctgccgcat tttcggcagc gcttcttcgg
     5101 taaggcgttt ttcatccgaa acggtgaggc gggtttcctg actttcattg gagccgtcgc
     5161 gttgccgcga actgttcgcg ggggcgcagg ctatgatcat caacaaaatg agcagaccaa
     5221 ccggaatgat tctctttttc atcactttcc tcccggagtc cataatgctt tgcttctctg
     5281 actccgccag gatctaagca tgaccgcctg cggctgtcaa gcggatgcgg ccgccgcgcg
     5341 ttcttccaac ggtttggtgt ttgacgcctc ttcccttctt ttggtataca aattccgtga
     5401 aaaacttgaa tgcgcggtct ttaaccgcga ccgttccggc agaagatctg catgccaccg
     5461 gttatcagca gaattttttt aggtgccgat gcgaacggcg gccccgcctg ccgtcgctgc
     5521 cggaatccgc aaggaggttc atcatgaaaa aaacaatcct tccgatcgtt ttgttttgtc
     5581 ttgcggcatg gtttccgcta ccggccgcgc acgcggatga gatcacgctg gaaaacggat
     5641 ccgccatcat tggaacgatt acccggattg atcaaaaagc gattctggtc cggacggatt
     5701 ttgccggagt tttgagcgtg gatatgacgc gggtgcttac ctacacgacg gaccatcccg
     5761 tttatgtggc ttttcccggc cacgatcccg tccgtggaac acttacctat accagaaaac
     5821 aaacgcgcat cgacgcggcg gatggaaaag tggtcgtgag cggcgatgcc ccggaacatg
     5881 tctggcctga aggcgcaacc gatccgcggg cccggcactg gggctttgag gtcggattgg
     5941 acgcgggggg aaagacgggc aacgccgagc gcctcagcgt cggttcaagg gcgcaggcca
     6001 aactgcaggg gcccagcgac cgcctgctcc tctacctgcg ctatgcttat gcaaaagatg
     6061 acggcatcga aagcgcaaac cagacggccg gcggaatcga tttcgaggcc tatttccggg
     6121 aaaaacactc ctggtatgcg cgcgtggaag cggaaaacga tcagatcgcc gccgttgact
     6181 ttcgggccac ggccgccgcc ggatacggct gctactttct gaaaaaaacc catcacacgc
     6241 tgcggggaag aaccggtctg atgttccgtc acgagacgtt gaagcatgaa tcctcgcggg
     6301 ccacattcgg gctcgatctg ggcatatccc acctgtaccg tttcgccaac gcctggcgtc
     6361 tgaccaatga cgtcacctac acgcccagcc tgcaggatta tctcgattac cggatttacc
     6421 atgaaagcgc gtttgatatt ccggttgtcg cctccgaagt ctggaaactc cgcctcggcg
     6481 tcagcaacga ttacaacagc aaaccggccg aaaataaagt ttatctggat accacctact
     6541 tcacccggct gatcttttcc tggcggtaaa ccggccggac aaggcgatat ttgctcttta
     6601 aaatcattta tgatacacga ccgtccaagc ttcaggaagg gaggcgcccg tgtcccgcag
     6661 aaccggaatt gtcaaggatg gccgctactt gcagcatgca accggaatgg atcatccgga
     6721 aagcccggag cggctggcgg cggtttatga gatgctggac aacccgcaca tgtcctggaa
     6781 atatgaaagc atcgaacccc gcgaggcctc tcacgaagaa atcgcttatg ttcataccct
     6841 gccctatatt caggagattg ccggcaccgc cggaaaggac tgcgtttttc tcgattccga
     6901 caccatcgcc ggcccggaaa gttatgaaat cgccaaactg gcggcgggcg gcatgatcaa
     6961 cgccatcgac gccgtcataa ccggcgaggt ggataatgcc tttgcgtttg tgcgtccgcc
     7021 cggccatcac gcaggcgccg gaaattccga gggcttctgc atcttcaaca atgtcgccat
     7081 cggcgcgatg catgccatga aaaagcacgg cttgaaaaaa atcctcattg tggactggga
     7141 cctgcatcac ggcaacggga cgcagaaaat gttttattcc gaccggcgcg ttctgtattt
     7201 ttccacgcat cagtatccct actacccggg aacggggagc ctgatggaaa tcggagaagg
     7261 ccaggcgctg ggctacacgg tgaacgtgcc tcttcggcgc ggagcggtca acgggacgtt
     7321 tgtctcggcg tttcgccgga ttctggagcc gattgcgaat gcctttgcgc cggagctgat
     7381 tctggtctcc gcgggctacg acatttacta ccaggatccg ctgggcggga t
//
LOCUS       DAOSIM010000096         7307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_9318_length_7307_cov_8.080116, whole genome shotgun sequence.
ACCESSION   DAOSIM010000096 DAOSIM010000000
VERSION     DAOSIM010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7307
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9318_length_7307_cov_8.080116"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..957
                     /locus_tag="PLR47_05455"
     CDS             226..957
                     /locus_tag="PLR47_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12424.1"
                     /translation="MPVYIGFDDTDILGADTGTGKLARNFERLIPAPCRVLGVVRQQL
                     LVDPAIPYTSHNSSACVILDDMDPALEDRLLALAVEHLELSALPGSDPGLCFARSDHS
                     RMAALQEFGLKCTRQIVTQREALRAAASIHLSGHGGTQDGIIGAAAAVGLTASGWRGR
                     FIEFSGLRSLPDPVSVSALAQKGIRVVSLDRDAITPCPRDEVHTEGWLRPRLWGSQAV
                     LPVLPSGPGRWKSMGGKRNKDHTRK"
     gene            970..1191
                     /locus_tag="PLR47_05460"
     CDS             970..1191
                     /locus_tag="PLR47_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12425.1"
                     /translation="MAGKIFYRERLKVGVKEKKPRFKLVAVAGVDITFYGDHLRKKEL
                     EEIAEAVGAELVLLKAQKKEKDEEVEVKD"
     gene            1208..1783
                     /locus_tag="PLR47_05465"
     CDS             1208..1783
                     /locus_tag="PLR47_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12426.1"
                     /translation="MALVRERDGRRLHVKSRLMGESLVGKTFMDGLTVAPQKRLFPDV
                     NVVKIGGQSICDRGAQALPAIVKEIAACSKKHKILLTTGGGTRSRHIYSIGLELGMPT
                     GVIAKFGSSISEQNALLVAMLLTPWGGIKIGHDDVTKLYTYFSQDCIPVMHGMPPYDY
                     FALPVPKRRIPIHRTDVGTLIAAWKFCKTAR"
     gene            1903..3237
                     /locus_tag="PLR47_05470"
     CDS             1903..3237
                     /locus_tag="PLR47_05470"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG12427.1"
                     /translation="MEEIKRASRKELFGWAMFDFANSSYTTVIITVVFCVIFSRLIVG
                     DGPEFRLGNLLWSASISISYLIVLLTAPLLGAIMDYTASKKKFLLASCLLCALATASL
                     YFIRPGDIPAAMLLIIVSNVGFSYSEAFISSFLPGLGPSRDLGKISGYAWGLGYMGGL
                     VSTAIVIFGLGAGVYTLANFSNLRWVGPATGLFFIIAAIPTFLWVRERTIARAMPPGE
                     NFFSIGVKRLKKTFIDIRDYRDLAVLLASFFFAYAGLSIVIAFAFIYGDQVIRWAGST
                     QILMFVLTQITAAGGALLFGLLQDSWGAKRTFILTLLLWVAAVSLIYGVSPVTEWLSG
                     LAGTPLKEEHVFLMIGAIAGLGLGSTQSACRAMVGLFSPDTKAGEFFGLWSLTSRLSA
                     IAGLMGLGVLQAWLGLHRAILVCSAFFFIAVVIVLFVDEERGKRTARAHAGE"
     gene            complement(3254..4024)
                     /locus_tag="PLR47_05475"
     CDS             complement(3254..4024)
                     /locus_tag="PLR47_05475"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOG12428.1"
                     /translation="MTAFNEGLSLRRLYERLMPAAKDRRIADLRIGLSYAGVLMDNGA
                     AGLAAVLPDSPPRGCTVLQGAGTFAGSSASDVLQYLVHGKNALHRAMGVAAANALAGS
                     PGKPAEDREATTYFDLKPGENVAMVGLFAPLVERIRKTGAKLTVIEKNPERIDLLSAE
                     DRRRALGECNVAIITATTLLNSTFEETIGLLGAPRIVALMGPSTPLVPEAFKDTPVTH
                     LGGAVVADCSRVLRIISEGGGTPALRPYLRFVNLTIGA"
     gene            complement(4021..4617)
                     /locus_tag="PLR47_05480"
     CDS             complement(4021..4617)
                     /locus_tag="PLR47_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HOG12429.1"
                     /translation="MNICTYSYEEYIHLVKSFHGTLAPGLLIGGFIVDLAMKHLPEGE
                     FFDAVCETPVCLPDAVQILTPCTIGNGWLSIVPFGKFAVTLYEKYSGKGVRVYLDTKK
                     LEAWPEVRDWYFKKKKKDEQDAEALFAQIREAGHRLLSVRHVQVEPEKVRRKKMGPVA
                     VCPVCGEAYPTRDGDKCRSCRGETPYTDVVAENEKEKI"
     gene            complement(4627..>4963)
                     /locus_tag="PLR47_05485"
     CDS             complement(4627..>4963)
                     /locus_tag="PLR47_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG12430.1"
                     /translation="MVSGGERQRCAICQALARHPRLLLLDEPFSALDVVTRRGLREEL
                     KSLKGRLSCPVIYVTHDIHEALFLADEILPVVDGRIDRGWMQRTITREPGEGAAARGA
                     REPRLSLVY"
     assembly_gap    4964..4987
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4988..5269)
                     /locus_tag="PLR47_05490"
     CDS             complement(<4988..5269)
                     /locus_tag="PLR47_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG12431.1"
                     /translation="MILSASFSKRLKYFNLELSLSCRPGKMAVMIGPSGGGKTTVIRM
                     LAGLTAPDAGRVVFGDEVWFDSARNIHLKPQKRRLGYVFQDYSLFPHLNL"
     gene            complement(5266..5925)
                     /gene="modB"
                     /locus_tag="PLR47_05495"
     CDS             complement(5266..5925)
                     /gene="modB"
                     /locus_tag="PLR47_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HOG12432.1"
                     /translation="MDYLALYVTLKLALATTIVLMILAAPVAYLLAYARFPGKSLVEA
                     LIYLPMALPPTVIGFYLIILMGPRGWAGRVWEAATGGSLLFTFLGITIASVIYSIPFA
                     VQPMKAAFSRIDRRLLESAYILGLSKRAAFLRVIIPNSVSGIAAAAVLVFLHSIGAFG
                     VLLMVGGSVPGETKVASIAIYEAVEMMNYEAAGLIALSFIPISYAFLLLVNRLNARAG
                     A"
     gene            complement(5928..6674)
                     /gene="modA"
                     /locus_tag="PLR47_05500"
     CDS             complement(5928..6674)
                     /gene="modA"
                     /locus_tag="PLR47_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG12433.1"
                     /translation="MKKTILVIVLLLSVLAAPFQVPAQDKISVGVAANFISAFKEIAA
                     AFEEQTGVKVEATFSSTGNLYSQIKNGAPYDVFLAADEKRPDLLHQEGLADGTFIYAR
                     GQAILWSADPNFCKAPTWREALKNDRIKKISIANPVTAPYGAAAKSALQKAGLWDALQ
                     SKLVNGQDIAQAFQYANTGAVDAGFCALSAAASREGKNGCYFSITEAPDIVQAGCVLK
                     KTKNPKSAELFMAYLLGADAVKTKKKYGYR"
     gene            complement(6772..>7307)
                     /locus_tag="PLR47_05505"
     CDS             complement(6772..>7307)
                     /locus_tag="PLR47_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HOG12434.1"
                     /translation="LAPSMAGIKYARLENDGLQWPCPGAEHPGTPTLHKGGKFTRGLG
                     MLKAIDYIPPAEVPDPEYPFVLSTGRRLYHYHTRTQTGRSAGINDLLGEETADISAAD
                     AQAKGISDGEKISVKSRRGEVVVKARVTKEIPPGLVWMAFHFREACANWLTNPVYDPV
                     TQTAEYKACAVRIDKLR"
BASE COUNT         1509 a         2154 c         2100 g         1520 t
ORIGIN      
        1 ctatttatgt catatttgac ataagaaaaa agtcctgtca gatcgtagca atgcggtttt
       61 tcacccaagg gatgaaagag cggatgactt ttggtcagaa cattgaaaac cctcgtgcgc
      121 cctgcgggat tcgtttcgat tcgccgaatt gagttgacac aaatcagcgg caactttata
      181 gtgcaaaagc gctggtaaag gcggccatgg aaggtaaaaa agcacatgcc cgtttacatt
      241 ggttttgacg atacggatat tttgggggcg gacaccggaa cggggaaact ggcccgcaac
      301 ttcgaacggc tgatcccggc cccgtgcaga gtgctggggg tcgtgcgcca gcagcttctg
      361 gtggatcccg ccatccccta cacatcccac aacagctccg cctgcgtcat ccttgacgac
      421 atggatccgg ctctcgagga tcggctgctt gcacttgccg ttgaacacct cgagctctca
      481 gcgctgccgg gaagcgatcc ggggctgtgc tttgcgcgct cggatcattc gagaatggct
      541 gcccttcagg agttcggcct gaaatgcacc cggcaaatcg tcactcagcg tgaagccttg
      601 cgggcggcgg cttcgattca cctctccggc cacggaggca ctcaggacgg catcatcggc
      661 gcggccgcgg cggtggggct gaccgcttcc ggctggcgcg gccggttcat cgaattttcc
      721 ggattgcgaa gcttgcccga tccggtttcc gtatcggcgc tggcgcaaaa agggattcgg
      781 gtggtttccc tggaccggga cgcaatcacg ccttgtcccc gggatgaagt tcacaccgaa
      841 ggatggcttc gcccgaggct ctggggaagc caggccgtcc tgcccgtgct gccttcaggc
      901 ccgggccgct ggaaaagcat ggggggaaaa agaaacaagg accatacaag gaaataagga
      961 ggaatgaaca tggctggaaa gattttttac cgggaacggc tgaaggtcgg cgtcaaggaa
     1021 aaaaagcccc gctttaaact ggtggcggtg gccggcgtgg atatcacctt ctacggcgac
     1081 cacctgcgga aaaaggagct cgaagaaatc gccgaagccg ttggggcgga gctggtgctg
     1141 ctcaaggcgc agaaaaagga aaaggacgaa gaggtcgaag tcaaagactg aggaggaacg
     1201 aagaagcatg gccctggtga gagaacggga cggcaggcgg ctgcatgtca aaagcagact
     1261 gatgggagaa agcctggtgg gaaaaacctt tatggacggg ctcaccgtgg cgccgcagaa
     1321 aaggcttttc ccggatgtca acgtagtcaa gatcggcggg cagtccatct gcgaccgggg
     1381 cgcgcaggcg cttcccgcga tcgtaaaaga aatcgccgcc tgctccaaaa agcacaagat
     1441 tctgctcacg accggcggcg ggacgcgaag ccgccacatc tactccatcg gcctcgaact
     1501 gggcatgccg acgggcgtca tcgccaaatt cggcagctcc atctccgagc agaacgccct
     1561 cctggtcgcc atgctgctga ccccctgggg cggcatcaaa atcggccatg acgacgtcac
     1621 caagctctac acctactttt cccaggactg cattcccgtc atgcacggaa tgccgcccta
     1681 cgattatttc gctctgcccg tacccaaacg gcgcattccc attcaccgca ccgacgtcgg
     1741 cacgctgatc gccgcctgga aattctgcaa aacagcgagg tgatccggac gattcaaatc
     1801 atcaacggcc tggagagagg aaatctgacc aaggcgctga atggaaagcc cgtgggaacc
     1861 ctcattcacc ggtcttgagc cgtccaggcc aaggaaaggc caatggaaga aatcaaaaga
     1921 gcttcacgca aagaactgtt cggatgggcc atgtttgatt tcgccaactc atcctatacg
     1981 accgtgatca tcacggtcgt gttttgcgtc attttttccc gtctcatcgt cggagacggg
     2041 ccggaattcc ggttgggcaa tctcctgtgg agcgcctcca tctccataag ctacctgatc
     2101 gtgcttttga ccgcgccgct tctgggcgcg atcatggact acaccgcgtc caaaaagaaa
     2161 tttctcctgg caagctgcct gctttgcgcg ctcgccaccg cttctcttta ttttatcaga
     2221 ccgggcgaca tcccggcggc gatgctgctc atcatcgttt ccaacgtcgg cttttcctac
     2281 agcgaagcgt ttatctccag ttttcttccg gggctgggtc cttcccggga cctggggaaa
     2341 atttcgggct acgcctgggg actgggctac atgggcggcc tggtttccac ggctatcgtg
     2401 attttcgggc tgggagcggg cgtttacacc ctggcaaatt tttccaatct gcggtgggtc
     2461 ggaccggcca cgggcctgtt ttttatcatc gccgcgatac cgacgtttct ctgggttcgg
     2521 gaaaggacga tcgccagggc catgccgcct ggtgaaaact ttttttcgat cggcgtcaaa
     2581 aggctcaaaa agaccttcat cgacattcgg gactacaggg atttggcggt tcttctggct
     2641 tccttctttt ttgcgtatgc cggcctctcc atcgtgatcg cttttgcctt catctacgga
     2701 gaccaggtca ttcgctgggc cggctcaacg cagatcctga tgtttgtcct cacgcagatt
     2761 acggcggcgg ggggcgcgct gcttttcggc cttctgcagg actcgtgggg cgcaaagcgg
     2821 accttcattc tgacgctcct tctctgggtg gcggccgtca gcctgattta cggcgtttct
     2881 cccgtaacgg aatggctgag cggtcttgcg ggaacacctt tgaaggaaga gcatgttttc
     2941 ctgatgatcg gagccattgc cggtctgggt ctgggatcga cgcagtccgc gtgccgcgcg
     3001 atggtcgggc ttttctcgcc ggacaccaaa gccggggagt tcttcggcct gtggagcctc
     3061 accagccgcc tgtccgccat cgcggggctc atgggactgg gcgtgctgca ggcgtggctc
     3121 ggccttcaca gagcgattct ggtctgttcc gcctttttct ttatcgccgt cgtgatcgtt
     3181 ttattcgtcg atgaagaacg gggcaagcga accgcgcgcg cccacgcggg ggaataatcc
     3241 gcggaccgct gcatcatgcg ccgatcgtca gattcacaaa ccgcaggtag ggacgaagag
     3301 ccggcgtgcc gccgccttcg gagatgatcc gcagaacgcg ggagcagtcg gcgacgacgg
     3361 cgccgcccag atgcgtgacg ggggtatcct tgaacgcttc gggcacaagc ggagtggaag
     3421 gacccatcaa cgcgacgatg cgcggcgctc ccaaaaggcc gatcgtttct tcaaaggtgc
     3481 tgttgagcag cgtcgtggcc gtgatgatgg cgacattgca ttcgcccagc gccctgcgcc
     3541 tgtcttccgc ggacagcaga tcgatgcgtt caggattttt ttcaatgacc gtcagcttcg
     3601 ccccggtttt gcggatgcgt tcgaccagcg gcgcaaaaag accgaccatc gcaacgtttt
     3661 cacccggttt caaatcaaaa taggtggttg cctcgcggtc ttccgccggt tttccggggc
     3721 ttcccgcaag cgcattggcg gccgctacgc ccatggcgcg atgcagggca tttttgccat
     3781 ggacgagata ctggagcaca tcggaagcgg atgaacccgc aaatgtcccc gcgccctgca
     3841 agacggtgca tccgcgcggc ggggaatccg gcagcacggc cgccagtccc gccgcgccgt
     3901 tatccatgag aacccccgcg tagctcaagc cgatgcgcaa atcggcgatc cgccggtctt
     3961 ttgcggccgg catgagcctc tcataaaggc ggcgcagcga gagaccctcg ttgaacgcgg
     4021 tcatatcttt tccttttcgt tttcggcaac cacatccgtg tagggcgttt cgccccggca
     4081 gctccggcat ttgtctccgt cgcgggtcgg ataggcctcg ccgcagaccg ggcagacggc
     4141 caccggaccc atttttttgc ggcggacttt ctccggttca acctgcacgt gccggacgct
     4201 gagcaggcgg tgtcccgctt cccgaatctg cgcgaaaagc gcttccgcat cctgctcatc
     4261 ttttttcttt tttttgaagt accagtcgcg cacttccggc caggcctcca gttttttcgt
     4321 atccagataa acccgcacac cctttccgct gtatttttca tacagcgtta cggcaaattt
     4381 gccgaaaggg acaatgctca accagccgtt gccgatcgtg cagggggtca aaatctgcac
     4441 ggcgtcggga aggcagaccg gcgtctcgca gacggcgtcg aaaaattccc cctccggcag
     4501 atgcttcatc gccagatcca cgatgaagcc gccgatgagc agccccggcg ccagcgtgcc
     4561 gtgaaaggat ttcaccaggt gaatgtattc ttcgtaggaa taggtacaga tgttcatcaa
     4621 ttctccttaa tagaccagcg acaggcgcgg ctcgcgcgct ccacgggccg ccgcaccctc
     4681 tcccggctcc cgggtgatgg tccgctgcat ccagccgcgg tcgattcttc cgtccacgac
     4741 cggcagaatc tcatcggcca ggaacagggc ttcatgaatg tcgtgcgtca cgtagatcac
     4801 gggacaggac agcctccctt tgaggctctt cagctcttcg cgcagcccgc ggcgcgtgac
     4861 cacatccagc gcggaaaaag gctcatcgag cagaagcaga cgcggatgac gggccagcgc
     4921 ctggcagatc gcgcagcgct gacgctcgcc gccggaaacc atgnnnnnnn nnnnnnnnnn
     4981 nnnnnnncaa attaagatgc ggaaaaaggc tgtagtcctg aaagacatac cccagccttc
     5041 gcttctgagg cttcagatga atattccttg cggaatcaaa ccagacttca tcgccgaaaa
     5101 ccacccgtcc ggcgtccggc gccgtaagcc ccgccagcat tcgaatgacc gtggtcttgc
     5161 cgccgccgga gggtccgatc atcacggcca tctttcccgg ccggcacgaa agcgaaagct
     5221 ccaggttgaa atatttcagt ctcttgctga acgaggcgga aaggatcatg cgccggccct
     5281 cgcgttgagt cggttgacga gcagcaaaaa ggcatagctg atcggaataa agctgagcgc
     5341 aatcagcccc gccgcctcgt agttcatcat ttcgacggcc tcataaatgg cgatggacgc
     5401 caccttggtt tctccgggaa cgctgccgcc gaccatcagc aaaacgccga atgcgccgat
     5461 cgagtgcaga aaaaccagca ccgccgcggc ggcgatgccg gaaaccgagt tgggaataat
     5521 gacccgcaaa aaggccgccc gcttcgacag gccgagaata taggcgcttt ccagcaggcg
     5581 gcggtcaatc ctggaaaagg ccgctttcat cggctgaacg gcaaacggaa tggaataaat
     5641 gacggacgcg atcgtgatgc ccagaaaggt gaacaaaagc gatccgccgg ttgcggcttc
     5701 ccatactcgc ccggcccagc cccggggtcc catcaggatg atcagataaa atccgatcac
     5761 ggtcggcggc agggccatcg gcagataaat gagcgcctcc acgagcgatt ttccggggaa
     5821 acgggcgtag gccagcagat aggccaccgg cgcggccaga atcatcaaaa cgatggttgt
     5881 tgccagggcc agtttcagcg tcacgtaaag agcgagataa tccatattta ccggtaaccg
     5941 tatttcttct tcgttttgac ggcgtccgcg ccaagcagat aggccataaa cagttcggcg
     6001 ctttttggat ttttggtttt tttcaaaacg cacccggcct gcacgatatc cggcgcttcc
     6061 gtaatgctga agtagcagcc gtttttcccc tctcggcttg ccgcggcgga aagagcgcag
     6121 aagccggcat ccacggcgcc cgtgttcgca tactggaacg cctgcgcaat atcctggccg
     6181 ttgaccagtt tgctctgcag cgcatcccac agtccggctt tttgcagggc cgacttcgcc
     6241 gccgcgccgt agggagcggt gacgggattg gcgatggaga tctttttgat ccggtcgttt
     6301 ttcagagctt cccgccaggt cggcgctttg caaaaattgg gatctgccga ccacaaaatg
     6361 gcctgaccgc gggcataaat aaacgtcccg tccgccaatc cttcctgatg aagcagatcc
     6421 ggccgctttt catccgcggc cagaaacacg tcataaggcg cgccgttctt gatctggctg
     6481 tacaggttgc ccgtcgaaga aaatgtcgct tccaccttca cgccggtctg ctcttcaaaa
     6541 gccgccgcga tctctttaaa ggcggaaatg aagttggccg ccactcccac ggaaatttta
     6601 tcctgggccg gaacctgaaa gggcgccgca aggacgctca acaaaagaac aataacaaga
     6661 atggtttttt tcatggcaac tgtttttctc tctttcaagt atgtctaaaa taacatagat
     6721 ttgcacaaaa aacatcccgc attttcgctg cgggatgttt ttttacggcc gttatctcaa
     6781 tttgtcgatc ctcaccgcgc aggccttgta ttccgccgtc tgcgttacgg gatcatatac
     6841 cggattggtg agccagttgg cgcaggcttc ccggaaatga aacgccatcc agaccaaacc
     6901 gggggggatt tccttcgtca cgcgcgcctt tacgaccacc tcgccgcgcc gggatttaac
     6961 ggagattttt tcaccgtcgg aaatgccttt ggcctgggca tcggccgcgg aaatatcggc
     7021 cgtctcctcg cccaacagat cgttgatgcc ggcggagcgc ccggtctgcg tccgcgtgtg
     7081 gtaatggtac aggcgtcttc cggtggagag cacaaacgga tactccggat cgggaacttc
     7141 cgcgggcgga atatagtcga tggctttcag catgcccaga ccgcgggtga attttccgcc
     7201 cttgtgcagc gtcggcgtgc ccggatgctc tgcgccgggg cacggccact gcagaccgtc
     7261 attttccaga cgggcgtatt tgattcccgc catggaaggc gccagca
//
LOCUS       DAOSIM010000097         7147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_9540_length_7147_cov_7.086153, whole genome shotgun sequence.
ACCESSION   DAOSIM010000097 DAOSIM010000000
VERSION     DAOSIM010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9540_length_7147_cov_7.086153"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PLR47_05510"
     CDS             <1..544
                     /locus_tag="PLR47_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOG12435.1"
                     /translation="LWVAVVAFVGNSAATLLLQKISARNFNIKSAWLHSLQDALFSLG
                     VIVGALLITFFDLYFVDPLISMVICIFIAREIYKIIRHTVNALLDAVPADVDFNAVKS
                     DLLSVAGVAEVNDLHIWQTGTGRKLLSVHLLSDNESPDHEQIIRAVQKILHETHGINH
                     TTIQLLPSSSREMEHCNHCN"
     gene            complement(643..1743)
                     /locus_tag="PLR47_05515"
     CDS             complement(643..1743)
                     /locus_tag="PLR47_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOG12436.1"
                     /translation="MVDVFNYHKSWILEVVSMFGLNNFITAGKFFLVIAGELILIFVA
                     VSFIIGLLMEYLPPSRVRDFLSNKFTWVQYFLGAGLGALTPFCSCSTVPITAGLLKGG
                     VPFGPTMAFLFSSPVLNPIIIALLLSLLGIKVTVFYVVFTFIGCMVMAALLSKLRMES
                     QIKPLLNIETSCCAASPDSHNASKSKPAAAACCSSDSAQPLTLITLPTVETSSCCSVN
                     LEPIAAPPVPFRQKLKNASIAAVDTFKGVFWYLLLGAAIGAFIYGFFPQDIVVKLAGP
                     GNPWSIPIAALIGVPMYIRAETIIPIAAALVGKGMGLGTVLALIIGGAGASIPELIIL
                     SSMFKRKLIIAFALNVLVIAVLAGYLVDIFLS"
     gene            complement(1736..2233)
                     /locus_tag="PLR47_05520"
     CDS             complement(1736..2233)
                     /locus_tag="PLR47_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOG12437.1"
                     /translation="MEDLTGQLAELLEDITYDFSAHNLSTNCCENISHGEFRALRAVS
                     RRDGCTMQDIARNIAVTKSGATRIVSRLEEKKLVLRVQERSDARICCVRLSKDGKAML
                     SRITEAPAKKISSVLDAMSPDMRQILLIGLRAFIETARAHGTIDGQLEEAESPVSKTG
                     NKIIG"
     gene            2487..3533
                     /locus_tag="PLR47_05525"
     CDS             2487..3533
                     /locus_tag="PLR47_05525"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG12438.1"
                     /translation="MEEFGTSSLIGCIRDWWEHQAMPRLDWMQIEITTRCNASCRYCP
                     RTVYCASWNDRDLSPAIFRSLLPALRRTRMVHLQGWGEPLLYPHFFDMIALAKKAGCM
                     VGTTTNGKLLDRRAAVRLVESRIDQVAFSLAGVGDQNDEARRGTHFSNVLEAVAGLAA
                     EKKVRRQHTPVISVAYLLLRSHLPDIDKLIPALAGTGIRQIVISTLDFACNENLQKEC
                     LAPEDKGQYRELMSLYDRLARDAEKEGISLFYNLVDPEAESGDCTENPHRAVFISASG
                     GVSPCAFTNIPVTESYHLSGAHQGYRSLIFGCLGPQSLPEIWSGRAAMKFRAEFDDAP
                     HPSCRGCSKRSRMK"
     gene            complement(3809..4015)
                     /locus_tag="PLR47_05530"
     CDS             complement(3809..4015)
                     /locus_tag="PLR47_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HOG12439.1"
                     /translation="MSSIYKVIEIIGGSEKSWEDAAKKAVETAAKSLKDIRIAEVKEM
                     DLRTEEGKVVEYRVKLRVSFKYTP"
     gene            4783..5970
                     /locus_tag="PLR47_05535"
     CDS             4783..5970
                     /locus_tag="PLR47_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG12440.1"
                     /translation="MKEKDKSESIFTKSIHAGEDRARYADSITTPIVQSSTYSFKDSR
                     EIEAYTKNDKKRYEYGRYGNPTEKVAERRLAELDGAEDGLMFSSGMMAITTTILALVQ
                     TGDHIVITDDCYGKTLEFCRSYLKRFGIECTIVPFGDYEVLDAAVKKNTRFIFSESPT
                     NPYLNVFDMVKFKKIGDKHKVITIIDSTFATPFNQRPLDYGIDLVIHSATKYLAGHND
                     ILAGVLLGKKELVSKVRELHKSMGGVIDPHCCYLLLRGLKTFPLRVKTQNENALKIAE
                     YLERHPKIERAYYPFLKSHDHYSIAKEQMRGGGGVVTFTVKGDLDAAKRFMDALEMIY
                     IAPSLGGVETLITHPATVTYYRYTREQRYDLGIVDNLFRLAVGIEDVEDIIADLERGF
                     AGL"
     gene            complement(5987..6814)
                     /locus_tag="PLR47_05540"
     CDS             complement(5987..6814)
                     /locus_tag="PLR47_05540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG12441.1"
                     /translation="MPHQNRFLGWENPGKALITGASAGIGLSFAKQLAQKGFDLILLA
                     RRKDRLESAAKKLESDYRIRCEIIAADLSDMDQIEKTADLIYTIEGLDVLINNAGFAT
                     IGPFAGVPLEKSMRMFHLHATACVMLTHAVLEGMLRRRRGAIINLSSVAAFALTPGNV
                     MYDATKAFVKTFSENLQLEMEDADVRIQALCPGFTRTEFHEVGDFIHFDRREIPDPLW
                     MSSDRVVSLSLKALENKRKTVFIPGWKNRFYVWLLQHSAVVRSIVQDSVKKRSQKKR"
BASE COUNT         1704 a         1784 c         1882 g         1777 t
ORIGIN      
        1 gctttgggtt gccgtggtgg cttttgtggg aaacagcgcg gccacgctgc ttctgcagaa
       61 aatatcggcc cggaacttca acatcaaaag cgcctggctt cattcgcttc aggatgcatt
      121 gttttctctt ggcgtgattg tcggcgctct gctgattacg ttttttgact tatattttgt
      181 cgatcctctt atatccatgg tcatctgcat cttcattgcc cgcgaaatct acaagattat
      241 ccggcatacc gtcaatgcgc ttctggatgc cgtgccggcg gacgtcgatt tcaatgccgt
      301 caagagcgat ttgctttccg ttgccggtgt ggccgaagtc aacgatctgc atatctggca
      361 aaccggaacg ggccggaaac tgctttccgt gcatctgtta tccgataacg aatcgcccga
      421 tcatgaacaa atcattcgcg ccgtgcagaa aatccttcac gaaactcacg gcatcaacca
      481 caccactatc cagcttttgc cgtcttcctc ccgcgagatg gagcattgca accactgcaa
      541 ttagtaactt ttgtgcgtct ttgcgagata cttggttcac ggtaagtttc ggcaacgcgc
      601 aaggcttttt gatggtacga taaaaatgca atctatttgc attcaggaca ggaatatatc
      661 cacaagataa ccagctaaga cggcaatgac aagaacgttt aaagcgaaag caatgatcag
      721 cttcctttta aacatggaac tgagaatgat caattcggga atgcttgcgc ccgctccgcc
      781 aataatcagc gcaaggacgg tgccaagtcc cattcctttt cccacaaggg cggctgctat
      841 gggtataatg gtttccgccc gaatgtacat gggaacgccg atcaaggccg cgatgggaat
      901 ggaccatggg tttcccggtc ccgccagttt gacaacgata tcctgtggga aaaaaccata
      961 aatgaaagcg ccgatggcgg cgcctaaaag gagataccag aacacaccct taaaggtatc
     1021 aacggcggcg atcgatgcat ttttgagctt ttgcctgaac ggcacaggcg gggcggcgat
     1081 cggttcaagg ttgactgaac agcatgatga tgtctcaacg gtcggcaggg tgattaacgt
     1141 caggggttgg gcgctgtctg aagagcagca agctgccgcc gcaggcttgg attttgaggc
     1201 attgtgtgaa tcaggagaag ctgcgcaaca ggaggtttca atattcaaaa gaggcttgat
     1261 ctgactttcc attctcaact tggatagcag cgctgccata accatgcagc cgataaaggt
     1321 aaagacaaca taaaaaaccg taactttgat gcccaacagt gaaagtaata gcgcgatgat
     1381 gatcgggttg agcaccggcg aggaaaaaag aaacgccatt gtcgggccga aaggcacgcc
     1441 gcctttgagc aggccggccg taatgggaac ggtggaacag gagcagaatg gggtgagtgc
     1501 gcccagaccg gcacccagaa aatattgcac ccatgtaaac ttgttggaca gaaaatcccg
     1561 gacgcgggac ggcggcagat attccatcag caagccgatg atgaaagaaa cggcgacaaa
     1621 tatgagaatg agttctcctg cgatcaccag aaagaatttt ccggccgtaa taaaattatt
     1681 taaaccaaac attgatacaa cctccagtat ccatgattta tgatagttga atacatcaac
     1741 caattatttt gtttccggtt tttgaaaccg gcgattccgc ttcttccagt tgaccgtcga
     1801 tcgtgccgtg ggcgcgggcg gtctcgatga aggcgcgcaa accgatgaga aggatctgcc
     1861 gcatgtccgg ggacatcgcg tccaggacag acgatatttt cttcgctggc gcctccgtta
     1921 ttctggacaa catggctttc ccatccttcg ataaccggac gcagcagatc ctggcgtcgc
     1981 tgcgctcctg cacgcggagg acaagttttt tttcctctaa tcgactgaca atacgcgttg
     2041 cgccgctttt ggtcacggca atgtttctgg cgatgtcttg catggtgcat ccgtcacggc
     2101 gggaaacggc acgtagagcg cgaaactcac cgtgggagat gttttcgcaa cagttggtgc
     2161 tcagattgtg tgcgctgaaa tcataggtga tatcttctag aagttcagca agttgtccgg
     2221 tgaggtcttc catggagcgt tctcctttgg tgacgatgaa acaatttcaa tcctgaagtg
     2281 gcatgaatag ttgaatatgt caactaatat ttggggcaat caaatttggg gagccatgcc
     2341 acatcgtctt acgctgcatc gctggttcga gtgaaagggg atgatttgac tcgcttttgt
     2401 aaatatgaga aaatttccaa gtagaaatgg actataaaat taatccggaa tcagacgcta
     2461 ggggcggtgt ttcagcagga ggtatcttgg aggaattcgg tacatcgtcg ttgatcgggt
     2521 gtatcaggga ctggtgggag catcaggcaa tgccccgact ggactggatg cagatcgaga
     2581 tcaccactcg ctgtaacgcc tcctgccgct attgtcccag gacggtttac tgcgcctctt
     2641 ggaatgaccg cgatctttct ccggcaatat tccggtcgct ccttcccgct ctgagacgga
     2701 cgcggatggt tcacctacag ggctggggtg agcctctact ttatccgcat tttttcgaca
     2761 tgattgctct ggccaaaaaa gccggttgta tggtgggtac cacaaccaac ggaaagctac
     2821 tcgatcgacg ggcggccgtc cgtcttgtcg aaagccgcat tgatcaggtt gctttctcac
     2881 tcgcgggtgt gggagatcaa aacgatgagg cgcgccgggg cactcatttt tccaatgttc
     2941 tcgaggcggt tgccggactc gccgcggaaa aaaaggtacg gcgacaacac acgccggtca
     3001 tcagcgttgc gtatctgctc ctgcgttcac atttgccgga catcgataaa ctgataccgg
     3061 cgctggccgg aaccggcatc cggcagatcg tgatcagcac cctggatttc gcctgtaacg
     3121 aaaatctgca aaaggaatgt ttggcaccgg aggacaaagg ccagtaccgg gaattgatgt
     3181 cactttacga cagacttgcc cgggatgcgg aaaaggaggg catttctctt ttttacaatc
     3241 tggtggatcc ggaggcggaa agtggggact gcacggaaaa tccgcaccgg gcggttttca
     3301 tctcggcgtc cggcggcgtt tccccctgtg catttaccaa cataccggtt acggaatcat
     3361 atcacctatc cggggctcat caaggatacc gttctctgat cttcggttgc cttggcccgc
     3421 agtcgctgcc ggaaatctgg agcggccgcg ctgccatgaa gtttcgggcg gaattcgacg
     3481 acgccccgca tccttcatgc cgcggttgtt ccaaacgaag ccggatgaag tgacggccgg
     3541 gtacttttca cgtttttggg aaaatactat ggttgcatca ttatcggcag gggaggtgtt
     3601 ccaatgaaat cgttctggct ttgttttgtg ctgttgtttg tagccgtgga tgccgtcggt
     3661 ttgccggcct tgtttatcaa tctgaccgaa ggcatccatc tctgagcgca cgtacctcac
     3721 cgttcctccc ggcggcggaa aaatatccgc cgccattcgc tgttggatga cagactgttg
     3781 accggtgttt gcccggcagg ggcgcttcct atggtgtgta cttaaaagat actcgcagct
     3841 tcacccgata ttcaacgacc ttgccttctt cggttctcag gtccatctct ttgacttccg
     3901 caatgcggat gtccttcaga ctcttggctg ccgtttccac ggcctttttg gctgcatctt
     3961 cccatgactt ttcactgccg ccgatgattt cgataacctt gtaaatgctg ctcatgattc
     4021 attctccttt ctcgtgaatg gttaaaaata ggtaaacgct gaaggccggc tttcgaagct
     4081 ggtcatgacg cgttgatgag ctttcatcaa atatatcgaa atcttacaag ttccccgtct
     4141 gggaatgatc ctgttgaaac aactggaagc gctcaagacg atggttccgc ggtcagggga
     4201 ggcttaggtt ttgaaaatct tcaggaaatc agggcaggca aagaagctgt gtaccggaaa
     4261 aagcgcagga tcaccaacga tcaattcctg cttttattat acctgtttaa gaaaaatcgt
     4321 caatcagaga aatgagtcag aaaaatgaag ctttagtgtc cgcaggatga gcagttcgat
     4381 gaactgcagg atgcgcaccc ggaactgctt ggtgcgctac tgctcgcgga tccgcccgac
     4441 ttcaatgatc cgtattttcc cttcccgact ttggaaacca gtttattggc ctttttggat
     4501 ttacatttcg ggcacacggg atttggtttt tcactgagac tcagagtaac cacttcatat
     4561 tgttttttgc atttactgca ttcgtattcg taaatcggca tatcttgccc ctttttttct
     4621 aaatctagag atcaagatgc aaaatgtcaa tacctgtgag gtcaattgtt gctgctaaat
     4681 gatctgcgaa attgtccatt gacataccca gaccttcttt ttatactgcc gcaaagtgaa
     4741 agcaagcctt gttcctcttc tttgcttgat ggaaaggaag ccatgaagga aaaagataaa
     4801 tcagaatcga tttttacaaa gtccatccat gcgggggagg accgggcgcg ctatgccgac
     4861 tcgatcacca ctcccattgt ccagtcgtcc acttattctt ttaaggacag cagggaaatt
     4921 gaagcctaca ccaagaatga taaaaaacgc tatgaatacg gccgatacgg caatccgacg
     4981 gaaaaggttg cggagcggcg tctggcggag ctggacggcg cggaggacgg gttgatgttt
     5041 tcttccggca tgatggccat taccaccacc attctcgccc tggtgcagac cggagaccac
     5101 attgtcatca ccgatgattg ctacgggaaa accctggaat tttgccgatc ctacctcaag
     5161 cggttcggca tcgaatgcac gatcgtgccg ttcggcgact atgaagttct ggacgccgcc
     5221 gtcaagaaga acacgcgttt tattttctcg gaatccccga ccaatccgta cctcaacgtt
     5281 tttgacatgg ttaaattcaa gaaaatcggg gacaagcaca aggtcatcac catcatcgat
     5341 tccacctttg ccacgccgtt caaccagcgg ccgctggatt acggcatcga tctggtgatt
     5401 cacagcgcga ccaagtatct tgccggccat aacgacattc tggccggcgt gcttctggga
     5461 aaaaaggagc tggtcagcaa agttcgggaa ctgcacaaat ccatgggcgg cgtgattgat
     5521 ccgcactgct gttatctgct tttgcggggg ctgaaaacgt ttccgctccg ggtgaagacg
     5581 caaaatgaaa acgctttgaa gatcgccgag tatctggagc ggcacccgaa gattgaaaga
     5641 gcctactatc cctttttgaa aagccatgat cattattcca tcgccaaaga gcagatgagg
     5701 ggcggaggag gtgtcgtcac ctttaccgtc aaaggcgatc tggacgcggc aaaaaggttc
     5761 atggatgcgc tcgagatgat ctatatcgcg cccagtctgg gcggcgtgga gacgctgatc
     5821 acgcatcccg cgacggtaac ctattaccgc tacacccggg agcagcgcta cgacctcggc
     5881 attgtggaca atctgttccg gctggccgtc ggcattgaag acgtcgagga tattattgcc
     5941 gatctggaaa gaggattcgc cggactctga acgcgattcc cgctttttaa cgtttcttct
     6001 gacttcgttt ttttacgctg tcttgaacga tggatcgcac gactgcgctg tgttgaagaa
     6061 gccatacgta gaaacggttc ttccaaccgg gaataaagac agtttttcgt ttgttttcca
     6121 gcgctttcag cgacagggac acgacccgat cggaagacat ccacagcgga tccggaatct
     6181 cgcgccggtc aaaatggata aaatcaccca cctcatgaaa ttccgtgcgc gtgaatcccg
     6241 ggcacagggc ctgaattcga acatcggcgt cttccatttc cagttgaagg ttttctgaaa
     6301 atgttttgac gaatgccttg gtcgcgtcat acatcacgtt gcccggcgtc agagcgaagg
     6361 cggcgacgga ggataggttg atgatcgcgc cccgtcttct tctcaacatg ccctcaagga
     6421 cggcatgcgt cagcatcacg caggccgttg cgtgcaggtg aaacatccgc atcgattttt
     6481 ccaggggaac cccggcaaag gggccgatgg tggcaaagcc ggcgttgttg atcaaaacat
     6541 ccaggccctc gatggtgtaa atgagatcgg ctgttttttc gatttgatcc atatcggaga
     6601 ggtccgcggc gataatctcg cagcggatgc ggtagtcgga ttccagcttt tttgccgcgc
     6661 tttccagccg atccttgcgc ctggccagca ggatcagatc gaaacccttt tgcgcaagtt
     6721 gcttggcgaa gctcagtcca atcccggccg atgctccggt gatcagcgct ttgcccggat
     6781 tctcccaacc caaaaaccga ttttgatgag gcatattaac ctccctttgg cccttttgat
     6841 ttccgaatca gactaatggc cgcatcatac gtcaatagga cgtttttggg aaagccaaat
     6901 tttttgtccg tgtgatttta caatactctt gcaacagaac ttttcactgt gcgggaaaag
     6961 tgctagaaag aaaacatcaa tcatgttcgt ggagggaggg ggctatgccg gatgcggtca
     7021 tcatcggggc ggggtacgcg ggaatgagcg ccgcggcgct tctttcccac gccggttgga
     7081 aggtgattgt tttggaagaa tccgaaatcg tcggcggccg cgcccgatcg tttcgggatg
     7141 aagaagg
//
LOCUS       DAOSIM010000098         6956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_9873_length_6956_cov_7.187799, whole genome shotgun sequence.
ACCESSION   DAOSIM010000098 DAOSIM010000000
VERSION     DAOSIM010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6956
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9873_length_6956_cov_7.187799"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /gene="ligA"
                     /locus_tag="PLR47_05545"
     CDS             complement(<1..1129)
                     /gene="ligA"
                     /locus_tag="PLR47_05545"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOG12442.1"
                     /translation="MVDLEDSSSKIMDKETAHRRIFELRKTIEYHNARYYQQDAPEIS
                     DAEYDRLMKELQNLEALYPDDELASSPTQRIGAAPLSKFAPFAHPQPMLSLANAFGAD
                     EIKDFDSRVRRLAKSERVDYVAEPKLDGLAVNLIYENGRFRRGATRGDGAVGEDVTQN
                     LKTIASLPLKMKTGAFRPIPEFIEVRGEVYMEKEPFEKLNRRRVEEGDAPFANPRNAA
                     AGSLRQLDSKITARRPLNIFLYGVGSAGDAAFASHWEILETLAAWGFPVNPLIRPMPD
                     INACIEYYEQTGSIRSSLPYDIDGVVLKVNRLEIQNLLGSVSRSPRWALACKFPAQQA
                     ATVIQDIIVQVGRTGTLTPVAVMEPVNVGGVLVSRATLHNED"
     gene            1248..1742
                     /locus_tag="PLR47_05550"
     CDS             1248..1742
                     /locus_tag="PLR47_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOG12443.1"
                     /translation="MKENKVKIGVLSDTHVHGFDPNLKRIIDENFSEVDLIFHAGDLV
                     DVSVLQLFGEKDVKAVCGNMDGPQVRERLPEQMIMDIGGFRVAMIHGWGSPAGLEGKL
                     AQKLGKVDCIVFGHTHYPVNKKIDGIYFFNPGSAMDRRFARHRSVGILEMGFEINGSI
                     INLK"
     gene            1812..2258
                     /locus_tag="PLR47_05555"
     CDS             1812..2258
                     /locus_tag="PLR47_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOG12444.1"
                     /translation="MCEKPEGCVFCKSSLRCDDYVVLEGKTCFVMLNRYPYVNGHLMI
                     IPLRHLGDLAALNPEERAEIFSLVDVSVKVLKEAMNPQGFNIGMNLGKAAGAGIAEHI
                     HVHVIPRWEGDTNFMSVVGNVRVIPQDLAATAAALIPLFEKYCREV"
     gene            2265..2582
                     /locus_tag="PLR47_05560"
     CDS             2265..2582
                     /locus_tag="PLR47_05560"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOG12445.1"
                     /translation="MKIFYLILTIVFAFFIVTFSQFNSAPVTIKYYYLQDLVVPAYML
                     IFVALLVGVVITGFLGVVERFRLNRTISKQNKVIRDLRKELREHEAPPMIEDSKKTHV
                     PPA"
     gene            2579..3070
                     /locus_tag="PLR47_05565"
     CDS             2579..3070
                     /locus_tag="PLR47_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOG12446.1"
                     /translation="MNTPTRFLADAALSGLARWLRLLGFDTVCVQEKAGRPLMRQAAG
                     EDRILLTRRRDLLERQFSGRLLLLPDTAVAKQLSFVLGKLSLEIEARNLYTLCLSCNQ
                     KMISLERRNVRDLVPEYVFEHCAEYTQCPECRKIFWPGTHRRNALRFLKDNGILVSGA
                     EGL"
     gene            complement(3101..5071)
                     /locus_tag="PLR47_05570"
     CDS             complement(3101..5071)
                     /locus_tag="PLR47_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG12447.1"
                     /translation="MGSRENQTRRGAGPEEALRERAGMLESILNGVLLLITYTDKDER
                     YAFVNRAYAEWYHIKPEELIGKRVSEVLKPDVYARAFENIQKALGGERVAYENQVTDP
                     EGRERILSVQYEPHFEGEAVKGFFTSIVDITGQKSAARELKERERVLATMIANLPGFV
                     YRCANDHDWTMTFLSEKCADVTGYTVSDFLQNKINYNDIVHPDYRESLREKWQEAILR
                     KGFFEEEYPVVAASGETRWVWERGRGVFDPAGELLFLEGFIGDITDRKRDEKEKEDLQ
                     TQLIQAQRMESIGRLAGGIAHDFNNMLAVILGRADMAMMRMDPAQRLYRDLQEIRKAA
                     ERSANLTRQLLAFARKQTVALKVLDLNEMAEGMLKILRRLIGENIDLVWQPGRNLPPV
                     KVDPDQMDQILVNLYVNAKDAIGGVGRIIMKTGAAALDEAFCAVHRGFVPGDYVTLSV
                     SDNGCGMNQETLNRIFEPFFTTKDTGRGTGLGLSTVYGVVKQHGGFINAESEPGRGST
                     FTVYLPRHLKAGIETAGNKETASVAGTETVLLVEDEPAILEMARMMLQRLGYTVLSAG
                     KPTEAMELARSHTGEIHLLMTDVIMPEMNGRDLALSISFFHPKIRRLFMSGYPANVIA
                     RQGVLDEGVHFIQKPFSMKDLAIKLREAIENR"
     gene            complement(5077..5274)
                     /locus_tag="PLR47_05575"
     CDS             complement(5077..5274)
                     /locus_tag="PLR47_05575"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOG12448.1"
                     /translation="MEPNVGPIDRIARLVIAVLFIVAVIADWIGGIAGVVLCVIAGML
                     LSSVVSGYCPLYVKLGLKKSF"
     gene            complement(5315..5809)
                     /locus_tag="PLR47_05580"
     CDS             complement(5315..5809)
                     /locus_tag="PLR47_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HOG12449.1"
                     /translation="MFNAGIITVSDKGSQGKREDKSGPVIAEMLAGAAIQIKQTLIIP
                     DEVAQIAKAIIQFADVEKLDLILTTGGTGVSPRDLTPDATLQVLDKEIPGIAEAMRAA
                     SMKVTPHAMISRAAAGIRGRSLIINLPGSPKGARENLSVVMPALTHAMEKIQGDDRDC
                     GVLP"
     gene            complement(5809..>6956)
                     /locus_tag="PLR47_05585"
     CDS             complement(5809..>6956)
                     /locus_tag="PLR47_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOG12450.1"
                     /translation="TIADPYAGRLTLFRIYSGKLNSDSAVYNSTRKITEKFGHIFFLE
                     GKNQKQAETLIPGDIAGVAKLKETVTGDTLCAEKYPIIFEKVAVPPPIMSFAVEPKSR
                     GDEDKIASSIHRLTEEDPTIVFSRNVQTKEMILSGMGQVHIEVNIEKMKRKFGVEVNL
                     NTPKVPYLETIKGKTNVQGRYKKQSGGRGQFGDCTIDIEPLPRGGGYEFLDKIVGGVI
                     PGQYRPAVDKGIQEAAAKGVIAGYPVVDFRISLVFGSYHTVDSSEMAFKIAGSMAFQK
                     GVMECQPILLEPIVNIAIEVPDEYMGDVIGDLNSRRGRVLGMDTNGTNQVIKGQVPLA
                     EILKYAPDLRSMTSGRGNFTYTDSHYEEVPSYIADKIIAESKKEAED"
BASE COUNT         1374 a         1905 c         2028 g         1649 t
ORIGIN      
        1 catcctcatt gtgcagcgtg gcccggctga ccagaacgcc gccgacattg accggctcca
       61 tcacggcgac gggcgtcagc gtgccggttc ttcccacctg gacgataatg tcctggataa
      121 ccgtggctgc ctgttgcgcc ggaaatttgc aggcgagcgc ccagcggggg gagcgcgata
      181 cgctgcccag aaggttttga atctccaggc ggttgacctt cagcacgacg ccgtcgatgt
      241 cgtaggggag gctggagcgg atcgagccgg tctgttcata atattcgatg cacgcgttga
      301 tgtccggcat gggccggatg agcggattga ccggaaaccc ccatgcggca agcgtttcga
      361 ggatttccca gtgactggca aaggctgcat cgccggccga cccgacgccg tagagaaaaa
      421 tattcagagg acggcgggcg gtgatcttcg aatccagctg gcgaagcgag ccggcggcgg
      481 cgttgcgcgg attggcaaaa ggagcatcgc cttcctctac ccggcgccgg ttgagttttt
      541 caaacggctc cttttccatg taaacttcac cgcggacttc gatgaattcc gggatggggc
      601 ggaaggcgcc ggtcttcatc ttcagcggca gggaggcgat ggtcttgagg ttctgcgtga
      661 cgtcttcacc cacggcgccg tcgccgcgcg tcgcgccgcg gcggaaccgt ccgttttcgt
      721 aaatcagatt cacggccagg ccgtccagtt tgggctccgc cacataatcc acccgctccg
      781 atttcgccag gcggcggacg cgcgaatcaa aatctttgat ttcatcggcg ccgaaggcgt
      841 tggccagact gagcatgggc tgagggtgag cgaagggggc aaatttggaa agcggggcgg
      901 cgccgatgcg ctgcgtcggg gacgaggcca gttcgtcgtc cggatagagg gcctccaggt
      961 tctgcagctc cttcatcaga cggtcgtatt cggcgtcgga tatttccggc gcgtcctgct
     1021 ggtagtagcg ggcgttgtga tattcgatgg ttttgcgcag ttcgaaaatg cggcggtgag
     1081 ccgtttcttt gtccatgatt ttacttgaag aatcttccag atcgaccatg gtccaaattc
     1141 tagccctcct tgtgatcaaa gtcaattcaa aagcattccg cgcaagggtc ttcgtgaaat
     1201 aacgcttgta atttttttat cttaccgata agaaaatcca cggggcgatg aaggaaaata
     1261 aagtcaaaat cggtgtgttg tccgatacgc atgttcatgg gttcgacccg aacctgaagc
     1321 ggatcatcga tgaaaatttt tcggaggtcg acctgatatt tcatgcgggg gacctggtgg
     1381 atgtatccgt tcttcagctt ttcggcgaaa aggacgtaaa ggcggtttgc ggcaacatgg
     1441 acggcccgca ggtcagagag aggctgcccg agcaaatgat catggacatc ggcggatttc
     1501 gcgtcgcaat gattcacggc tgggggtcgc ccgcgggcct ggaaggaaag ctggcccaaa
     1561 aactggggaa ggtggactgc atcgttttcg gccacacgca ttacccggtc aacaaaaaaa
     1621 tcgacggcat ttattttttc aatccgggtt cggcgatgga ccggcggttt gccagacacc
     1681 ggagcgtcgg aattttggaa atgggcttcg aaatcaacgg gagcattatc aacctcaaat
     1741 agcgcgatag accgcgatca ggtaccggac agatggaaac aatgtgtgcg ccgtggcgaa
     1801 tggagtatct catgtgtgaa aaaccggaag gatgcgtgtt ttgcaaaagt tcccttcgct
     1861 gcgatgatta tgtggtgctg gaagggaaga cctgctttgt catgctgaac cgttaccctt
     1921 acgtcaacgg tcatctgatg atcattccgc tgcgccatct gggcgacctt gcggcgctga
     1981 accctgaaga gcgggcggaa attttcagcc tggtggacgt ctccgtgaaa gttctgaagg
     2041 aggcgatgaa cccccagggc ttcaacatcg gcatgaacct gggcaaggcg gccggcgccg
     2101 gaatcgccga gcatatccat gttcacgtga ttccgcgctg ggagggagac accaatttca
     2161 tgagcgtcgt cggcaatgtc cgcgttattc cgcaggacct ggccgccacc gccgcggccc
     2221 tgatccccct gtttgaaaaa tattgccggg aggtttaaaa cgagatgaag atattctatt
     2281 taatcctcac cattgtgttc gcttttttta tcgtgacctt ttcacaattc aactcggcgc
     2341 ccgtgaccat caagtactat taccttcagg atctggtggt tccggcctac atgctcattt
     2401 ttgtcgcgct tctggtgggc gtcgtcataa cgggatttct gggcgtggtg gaaagatttc
     2461 ggctcaaccg caccatctcc aagcagaaca aggtgattcg cgatctgcgc aaggagctga
     2521 gggaacacga agccccgccg atgattgaag acagcaagaa aacacacgtt cctccggcat
     2581 gaatactcca accagatttc tcgccgacgc agcgctttcg ggactggccc ggtggctcag
     2641 gctgctggga tttgacacgg tctgcgttca ggagaaagcg ggccggccgt tgatgaggca
     2701 ggcggcgggc gaagaccgga ttttgcttac ccgcagaaga gatctcctgg agcgtcagtt
     2761 ttccgggcgt ctgctgcttc tgccggacac ggccgttgca aagcagcttt cttttgtgct
     2821 ggggaagctg tcgctggaaa tcgaggcccg aaacctttac acgctttgtc tttcctgcaa
     2881 tcaaaaaatg atttccctgg agcgccggaa tgttcgggac ctcgtgccgg aatatgtttt
     2941 tgagcattgc gccgaataca cgcagtgtcc cgaatgccgg aaaattttct ggcccggaac
     3001 gcaccggcgc aacgccctgc ggtttttgaa agacaacggc atcctggtgt ccggggcgga
     3061 aggcctgtga aaaaaagtca ggcggcgccg cgggattttc ttaccggttt tcgatggcct
     3121 cgcgcagttt gatcgccaga tccttcatcg aaaacggctt ctgaatgaaa tgcactccct
     3181 cgtccaggac gccctggcgg gcgatgacgt tggccgggta cccggacatg aaaaggcgcc
     3241 ggattttcgg atggaagaat gaaatgctca gggccaggtc ccgcccgttc atttcaggca
     3301 tgatcacatc agtcatcaga agatgaatct ctccggtgtg gctgcgggcc agctccattg
     3361 cttccgtcgg ttttccggcc gaaagaacgg tgtagcccag acgctggagc atcattcggg
     3421 ccatttccag aatcgcaggt tcgtcctcta ccagcagaac ggtttccgtt ccggcgacgg
     3481 atgccgtttc tttatttccg gccgtttcaa tgcctgcttt caggtggcgg ggcaaataga
     3541 ccgtgaaggt cgagccccgg cccggttcac tctccgcgtt gatgaagccg ccgtgctgct
     3601 tgacgacgcc gtatacggtg gacaatccca ggcctgtgcc ccggcccgta tccttggtgg
     3661 tgaagaaagg ttcaaaaatc ctgttcagcg tttcttggtt cattccgcag ccgttgtcgc
     3721 tgacggaaag cgtcacgtag tctccgggga caaagcccct gtgtacggcg cagaaagctt
     3781 cgtcgagcgc cgcggccccc gttttcatga tgatcctgcc tacgcctcca atggcatctt
     3841 ttgcgttgac gtacagattc accaggatct ggtccatctg gtccggatcg actttgaccg
     3901 gaggcagatt cctgcccggc tgccagacga gatcgatatt ttcgccgatg agccttcgaa
     3961 ggatcttcag cattccctcc gccatttcgt tcagatcgag gaccttcagc gccacggtct
     4021 gtttgcgcgc gaaggccaaa agctggcggg tcagattggc cgagcgttca gcggctttcc
     4081 ggatttcctg caaatccctg taaagacgct gggcggggtc catccgcatc atggccatgt
     4141 ccgcgcgccc caggatgacg gcgagcatgt tgttgaaatc atgggcgatg cctccggcga
     4201 gtcttccaat ggactccatc ctttgcgcct ggatcagctg tgtctgcaga tcttcctttt
     4261 ccttctcgtc gcgcttgcgg tccgtgatgt ctccaatgaa gccttcgaga aagagcaatt
     4321 ctcccgccgg atcaaaaacg ccgcgtccgc gctcccacac ccatctggtt tctcccgagg
     4381 cggcgaccac cggatactct tcctcgaaaa aacctttccg caggatcgcc tcctgccact
     4441 tttctctcaa ggattcgcga taatccggat gaacgatgtc gttatagttg attttgtttt
     4501 ggaggaaatc ggagacggta tatccggtga cgtcggcgca cttctcgctc aggaacgtca
     4561 tcgtccagtc gtggtcgttt gcgcagcggt aaacgaatcc gggaaggttg gcgatcatcg
     4621 tagccagcac ccgttcccgc tccttgagct cccgggctgc gctcttctgc ccggtgatgt
     4681 ccacgatgga ggtgaagaat ccttttaccg cttcgccttc gaaatgcggt tcatactgaa
     4741 cgctgaggat tctttcccgg ccttccggat cggtcacctg gttttcgtaa gcgacacgct
     4801 cgccgccgag cgccttctga atgttttcga aggccctggc gtagacgtcc ggttttaaaa
     4861 cttcggaaac ccttttcccg atcagctctt ccggcttgat gtgataccac tcggcgtagg
     4921 cccggttcac aaaagcgtat cgttcatctt tgtcggtgta cgtgatgagc agcaacacgc
     4981 cgtttaagat gctttcgagc atcccggctc tttcacgcag ggcctcttcg ggaccggcgc
     5041 cgcgtctggt ctggttttcc cggcttccca tcctggtcag aaggatttct tcaagcccag
     5101 ttttacgtat agcgggcagt atcccgacac cacgctggag agaagcattc cggcgatgac
     5161 gcaaaggaca acgccggcaa tgccgccgat ccagtccgcg atgacggcga cgatgaatag
     5221 aacggcgatg acgagacgag cgatccggtc gatgggtccc acgtttggtt ccattggcga
     5281 tcctccttga aacggtaaga ggttttcggg ttggttacgg caataccccg caatccctgt
     5341 cgtcgccctg gattttttcc atggcgtggg tcagcgcggg catcacgacg gaaaggtttt
     5401 cccgggcccc cttgggactg ccggggaggt tgatgatcag ggatcggccg cggatgccgg
     5461 ccgccgcgcg ggagatcatg gcatgcggcg tcaccttcat gctggcggcc cgcatggctt
     5521 ccgctattcc cgggatttcc ttgtccagaa cctgaagcgt ggcgtccggc gtcaggtcgc
     5581 gcgggctgac gcccgtgccg ccggtcgtca gaatcagatc cagtttttca acgtccgcaa
     5641 actgaatgat ggctttggcg atttgcgcga cttcatcggg gataatgagg gtttgcttga
     5701 tttgaatagc ggcgcccgca agcatttctg cgatgacggg gccgcttttg tcctcccgct
     5761 ttccctgcga gcctttgtcg ctgacggtga tgatgccggc attaaacatt aatcttccgc
     5821 ttcctttttt gattcggcga tgatcttgtc cgcgatgtaa gaggggactt cttcatagtg
     5881 cgaatcggtg taggtaaagt tgccgcgtcc gctggtcatg gaacgaagat cgggggcgta
     5941 tttcagaatt tccgcaagcg gcacctggcc tttgatgacc tgattggtgc cgttggtgtc
     6001 catgcccaaa acgcgcccgc ggcggctgtt gagatcgccg atgacgtctc ccatgtattc
     6061 atcgggaact tcgatggcaa tattgacgat cggttccagc agaatcggct ggcattccat
     6121 aacgcctttc tgaaacgcca tggagccggc gattttgaag gccatttccg aggagtccac
     6181 ggtatgataa gagccgaaca ccagggatat tctgaaatcc acgaccggat agccggcgat
     6241 gacgcccttg gcagccgctt cctgaatgcc tttgtctacg gccgggcggt attggccggg
     6301 aatgacgccg ccgactattt tgtccaggaa ttcatagcct ccgccgcgcg gcagcggttc
     6361 gatgtcgatc gtgcagtcgc cgaactggcc gcgtccgccg gattgcttct tatagcgtcc
     6421 ctggacgttg gtttttccct taatggtctc cagatagggg actttcggcg tgttgagatt
     6481 gacttcaacg ccgaatttcc gcttcatctt ttcaatgttg acttcgatgt gcacctggcc
     6541 catgccggag agaatcattt ctttggtctg gacgttgcgg gagaagacga tggtcggatc
     6601 ttcctccgtt aaacggtgaa tcgacgaagc aatcttgtct tcatcgcctc tggatttcgg
     6661 ttccacggca aacgacatga tcggcggcgg aacggcaact ttctcaaaga taatgggata
     6721 tttctcggcg cacagcgtgt cgccggtgac ggtttccttc agtttggcca cgcccgcgat
     6781 gtcgccggga atgagcgtct ccgcctgttt ctggtttttg ccttccagaa agaaaatatg
     6841 accgaatttt tccgtgattt tcctcgtgga attatatacg gccgagtcgg aattgagctt
     6901 tccggaatag atccggaaca gcgtcagccg gcccgcgtaa gggtcggcga tggttt
//
LOCUS       DAOSIM010000099         6934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_9912_length_6934_cov_9.754325, whole genome shotgun sequence.
ACCESSION   DAOSIM010000099 DAOSIM010000000
VERSION     DAOSIM010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6934
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9912_length_6934_cov_9.754325"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /gene="pyrG"
                     /locus_tag="PLR47_05590"
     CDS             complement(<1..378)
                     /gene="pyrG"
                     /locus_tag="PLR47_05590"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416741.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HOG12451.1"
                     /translation="MKNTKFIFVTGGVLSSLGKGLAAASIAAVLESRGLRVTNQKLDP
                     YINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFSSTCMGKRNNLTTGQVYFSVIT
                     KERRGDYLGKTVQVIPHITNEIKD"
     gene            complement(456..2624)
                     /locus_tag="PLR47_05595"
     CDS             complement(456..2624)
                     /locus_tag="PLR47_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOG12452.1"
                     /translation="MKKTKATAAAERHYVIEKEKFVPVSQYFGEDTFNHKVMKQKLPK
                     DTFRKLMEAMNEDKKLDHETANIVAHAMKEWALEKGATHFAHWFQPMTGITAEKHDAF
                     ADPCGPGAVIERFSGKQLVQGEPDASSFPSGGIRATFEARGYTAWDMSSPAFIKRNGI
                     STTLCIPTAFISYTGEALDKKTPLLRSSRAVSQSAVNMLKLLGAKNVRRVYSNLGPEQ
                     EYFLIDMDYYYKRQDLVLGGRTVVGAPPAKGQELEDQYFGSIRERISSYMHDVEEDLF
                     KLGIPAKTRHNEVAPSQYEIAPVFEEANLAVDHNQLVMDTLKSVARKHYLSALLHEKP
                     FARINGSGKHVNWSLADDNGNNLLNPGKTPQDNISFLVFLIAAVRAVYRHADILRASV
                     ASYANDHRLGANEAPPAIISVFLGEQLTQILDSIEKGKVAKATNEEIIDLGISSLPQV
                     SKDNTDRNRTSPFAFTGNKFEFRAVGSSQSISFPAAVINTIVAESLDDLAEKIRAKGS
                     KNINQAVFEVLKSEIRVIKPILFNGDNYTKAWEAEAGKRSLPNFKTTPAALGALVTDK
                     ALRLFEKYRVLTRVELQSRYIIYLEKYIKDLEIEVKCLGNICLNQVIPAAADYQKRVA
                     EAVIATKAALGGAAVAAEADLLKKVIRLVNTIYAGHKEIMAGMEAANAGHDEAKKAET
                     LCEKIKPRMDALRESVDELENLVDDERWPLPKFWEMLFIC"
     gene            complement(2654..4993)
                     /locus_tag="PLR47_05600"
     CDS             complement(2654..4993)
                     /locus_tag="PLR47_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG12453.1"
                     /translation="MMTKKDTIKISGVENQIRVDSRILEERMQKAVADGCRHIEVTAL
                     GQHGIGGRLWRAQNAPVKVDVLGTSGQRLGSMGFANTTIEVFGPASDDVGWLNAGAQI
                     IVHGNATNGTANAMAQGRVYIGGDIGARGMTMTKFNPRFEPPELWVFGKVGDSFAEFM
                     AGGIAVVCGAGAEEERNVLGYRPCVGMVGGKIFFRGKQEAYSTQDARLVADLPDFEWE
                     WLKQNLKTFLEAIGKQYLFEQLSEKREDWQLLIARKPSEKTARTVRPMAKFRREVWEN
                     ELGAGGLIGDLSSLDRSPIDVIATGVLRRYVPSWENGKYSPPCQAACPTGIPVQKRWE
                     LIRKGHLEEAVNLALEYTPFPAAVCGYLCPNLCMQNCTRSLVHLEPVDVTLLGKASLE
                     AKEPRPRPQTGKKVAVVGGGAAGLSVAWQLWLKGHETVIIESQKRLGGKIADAIPQSR
                     IPSDVVEHEIGRLAKSIHKVRLGKPLTKEQFARFKSEHDFVVIATGASRPRVLNIPGV
                     EKSVSALEFLRRSKLDCAKVGEKVVIIGAGNVGCDAASEAFRLGAKSVILIDVQEPAS
                     FGAERKQAEAAGAKFLWPRFTRAITDQGIELTGGEMLPAETVIVAVGDLPDLSFLPQS
                     IATEKGFVVVDKTYATSDPQVYAIGDAVRPGLLTDAIGAGRIAARSIDDRLRGAAETY
                     DKLPPIGQERVKLQYFDPRIRAFADTASCATNCASCGACRDCGLCEEICPQNAIFRKT
                     LPDGGFEYAVDGESCIGCGFCAGACPTGVWELSENKPLE"
     gene            complement(5059..>5403)
                     /locus_tag="PLR47_05605"
     CDS             complement(5059..>5403)
                     /locus_tag="PLR47_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOG12454.1"
                     /translation="AAALIMLGASGVQTGKYILQAAAGCLGSEGDRCNVCNIGVCPKG
                     ITSQDPRLDRRLDPEKVARRLVDVYLTFDTELKKIVAPLGRSTSLPIGMSDALGISNA
                     DAAQRLNIQYVL"
     assembly_gap    5404..5413
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5414..6709)
                     /locus_tag="PLR47_05610"
     CDS             complement(<5414..6709)
                     /locus_tag="PLR47_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOG12455.1"
                     /translation="MEPVERITPSTLSAKDLPWQILWRKDRCTLCGRCTSVCPVRAIE
                     LGVHRKRALTIPPGLEERPANLFSVYHGIDQRTDPAHACVGCGMCTLVCPNGAIVPAR
                     TDESDRLRFHVNQGGEARRRGGRRNDSKSVLDRIKFVRISMLTDPALDAGRHEFELHT
                     LLGRILPPERMLAAERDNGWTPPVREIYPLIIGGMSFGALSPNMWEGLQMGVAHLNEE
                     MGMPVRICTGEGGCPPRLLQSRFLKYVILQIASGYFGWDEIIHALPKMKEDPCAIEIK
                     YGQGAKPGDGGLLMWYKVNKLIAAIRGVPQGISLPSPPTHQTKYSIEEAVAKMIQSMS
                     MAWGFRVPVYPKISATSTTQAVLNNLTRNPYAAGLAIDGEDGGTGAAYNVSMDHMGHP
                     IASNLRDAYMTLVKLGKQNELPLFAGGGIGKNGNLAANAA"
     gene            complement(6673..>6934)
                     /locus_tag="PLR47_05615"
     CDS             complement(6673..>6934)
                     /locus_tag="PLR47_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HOG12456.1"
                     /translation="PNCVIGCLPDKSMFMVQDSKKLRPGVVGGRPGIFAFSSEMCGLD
                     ASIPERDINLDHQPMRYETVLVRRERQEMEKWNQWNALPPLH"
BASE COUNT         1247 a         2084 c         2020 g         1573 t
ORIGIN      
        1 atccttgatt tcgttcgtga tatgcgggat gacctgaacg gttttgccca ggtaatcgcc
       61 gcgtctttct ttggtaatga cggagaagta aacttgcccg gtcgtcaggt tgttgcgctt
      121 gcccatacag gttgaggaaa atctttcgta atgccccaga tccaaatccg tttcggcgcc
      181 gtcgtccgtg acgaagacct ctccgtgctg aaacgggctc attgtgccgg gatccacgtt
      241 gatgtaggga tccagcttct gattggtgac gcgaagaccg cgcgactcca ggacggccgc
      301 aatcgatgcc gccgccaggc ctttgcccag cgacgacaga acgccgccgg tcacaaaaat
      361 aaatttcgta tttttcacga tgggtccttt tgcaattcca tggtccctca aagagggcgg
      421 cagggaaaac cgtccaggcg ttccctgcct ttgtgtcagc agataaacaa catttcccag
      481 aacttgggca gaggccatct ctcgtcatcc accaggtttt ccagttcatc cacggattcc
      541 cgcagcgcat ccattctcgg tttgattttt tcgcagaggg tttccgcctt tttcgcttca
      601 tcatggccgg cgtttgccgc ctccatcccg gccatgattt ccttgtgtcc ggcgtagatg
      661 gtgttgacca gtcggatgac tttctttaag aggtcggctt cagcggcaac cgccgcgccg
      721 cccagcgccg ctttggtcgc gatcacggct tcggccaccc tcttctggta gtccgccgcc
      781 gccggaatga cctggttcag gcatatattg cccagacact tgacttcaat ttccaggtcc
      841 ttgatgtatt tttccagata aatgatatac cgggactgaa gttccacccg ggtgaggacc
      901 cggtatttct caaaaaggcg aagcgccttg tccgtgacca gcgcccccag cgccgccggc
      961 gttgtcttga aattcggcag actccgtttg ccggcttcgg cttcccacgc tttggtatag
     1021 ttgtcgccgt tgaacagaat cggcttgatg acccgaatct cgcttttcaa aacctcaaag
     1081 accgcctgat tgatgttttt cgatcctttg gccctgatct tttcggccag gtcgtccagg
     1141 ctttccgcca cgatggtatt gatgaccgcc gccggaaacg aaatggattg agacgaaccc
     1201 accgcgcgga attcaaactt gttgccggta aaggcaaacg gcgaagtgcg gttgcggtcg
     1261 gtattgtcct tgctgacctg cggcagagag gaaattccca gatcgatgat ttcttcgttg
     1321 gttgccttcg cgacttttcc cttttcgatg ctgtcgagga tctgcgtcag ctgttctccc
     1381 aggaagacgg aaataatcgc ggggggcgct tcattggcgc ccagccggtg gtcgtttgcg
     1441 taggacgcga cggaggcgcg cagaatatcg gcgtgcctgt aaacggctcg gaccgcagcg
     1501 atcagaaaca ccagaaacga aatgttgtcc tggggcgttt tgccggggtt gagcagattg
     1561 ttgccgttgt cgtcggcaag cgaccagttg acatgtttcc ccgatccgtt gattcgggca
     1621 aacggcttct catgcagcag cgcggataaa tagtgttttc tggccacgga cttcagcgta
     1681 tccatcacca gttgattatg atcgaccgcc agattggcct cttcaaacac gggcgcgatt
     1741 tcgtactggc tgggcgcgac ctcgttatgc ctggtcttgg ccggaatgcc cagtttgaaa
     1801 aggtcctctt ccacgtcatg catgtaggag gaaatcctct cgcgaatgct gccgaaatac
     1861 tgatcttcca gctcctgacc cttggccggc ggcgcgccga ccaccgtccg gccgcccagc
     1921 accagatcct gacgcttgta gtagtaatcc atgtcgatca gaaaatactc ctgctccggc
     1981 cccagattgg agtaaactct ccggacgttt ttggccccca gaagcttgag catgttcacg
     2041 gcgctttggc tgaccgcacg gctggagcgc aaaagaggcg tctttttatc cagcgcttct
     2101 cccgtgtagg aaatgaaggc ggtcggaatg caaagcgtcg tggagattcc gttgcgcttg
     2161 atgaaggcgg gcgaagacat gtcccacgcc gtgtagccgc gggcctcaaa ggtcgcccga
     2221 atgccgccgg aggggaaact cgatgcgtcc ggctccccct gcaccagctg cttgccggaa
     2281 aaccgctcga tcaccgcgcc ggggccgcag ggatcggcaa aggcgtcgtg cttttcggcg
     2341 gtgatgcccg tcatgggctg aaaccagtgc gcaaagtggg tcgcgccctt ttccagcgcc
     2401 cactccttca tggcgtgggc gacgatgttg gccgtttcgt gatccagctt tttatcttcg
     2461 ttcatcgctt ccatcagttt tcgaaaggtg tctttgggaa gcttctgctt catgacctta
     2521 tggttgaagg tgtcctcgcc gaaatactgc gaaacgggaa cgaacttctc tttttcgatc
     2581 acgtaatgtc tttccgccgc cgctgttgct tttgtctttt tcatgtcttt tttatcctta
     2641 gctaaaaagt tttttattcc aaaggcttgt tttccgaaag ttcccagact cccgtcggac
     2701 aggcgcccgc gcaaaaaccg cagccgatgc agctctcgcc gtcgacggca tattcaaatc
     2761 cgccgtccgg cagtgttttg cgaaagatcg cgttttgcgg gcatatttcc tcgcacaggc
     2821 cgcagtcgcg gcatgccccg caggaggcgc agttcgtcgc gcaggaagcc gtgtcggcaa
     2881 acgcgcgaat ccgcgggtcg aaatactgca gtttaacgcg ttcctggccg atcggcggca
     2941 gcttgtcgta ggtctcggcg gccccccgca gccggtcgtc gatcgaccgg gcggcaatgc
     3001 gtccggcgcc gatggcgtcg gtcagcagcc cgggacggac cgcgtcgccg atggcgtaaa
     3061 cctgcggatc gctcgtggcg tatgttttat ccaccacgac aaaccccttt tccgtcgcga
     3121 tgctctgcgg cagaaaggac aaatccggaa ggtccccgac ggcgacgatg acggtttccg
     3181 ccggcaacat ctcgccgccg gtcaattcga tgccctggtc cgtgatggcc ctggtgaagc
     3241 gcggccagag aaattttgcg cctgcggctt cggcctgttt tctctccgcg ccgaaggagg
     3301 ccggttcctg gacgtcaatc agaatgacgc ttttggcgcc caggcgaaag gcttccgacg
     3361 ccgcatcgca gcccacattg cccgcgccga taatcacgac tttttcaccg accttggcgc
     3421 agtccagttt gcttcgccgc aaaaactcca gcgcgcttac ggatttttcc acgccgggaa
     3481 tgttgaggac gcgcggccgc gacgcgcccg tggcaatcac gacaaaatca tgttcgcttt
     3541 tgaacctggc gaactgctct ttcgtcaaag gcttgcccag tcgaaccttg tgaatacttt
     3601 tggccagccg cccgatttca tgctccacca cgtcggaagg aatgcggctt tgcggaatgg
     3661 catccgctat tttccctccc agccgctttt ggctctcaat aatgacggtt tcatggcctt
     3721 tcagccagag ctgccaggcg acggaaaggc ccgccgctcc gccgccgacc accgccactt
     3781 tttttccggt ctgcgggcgg ggccggggtt ccttcgcttc cagagacgct tttcccagca
     3841 gggtaacgtc aaccggctcc agatggacca ggctgcgcgt gcagttctgc atacagaggt
     3901 tcggacacag atagccgcag acggccgcgg gaaagggggt gtactccagc gccagattca
     3961 cggcttcctc caggtggccc ttgcggatca gttcccatcg tttttgaacg ggaatgccgg
     4021 tcgggcaggc cgcctggcag ggcggcgaat attttccgtt ttcccaggaa gggacgtaac
     4081 gccgcagcac accggtggca atcacgtcga tcggagagcg gtccagactc gacaaatcgc
     4141 cgatcagccc tcctgcgccc agttcatttt cccagacctc ccggcgaaat tttgccatcg
     4201 ggcgaacggt ccgcgccgtt ttttcggacg gttttcgggc gatcaaaagc tgccagtctt
     4261 cgcgtttttc cgagagctgc tcaaacagat actgcttgcc gatggcctcc agaaatgttt
     4321 tgaggttttg cttcagccac tcccactcaa agtccggaag gtcggccacc aggcgggcat
     4381 cctgcgtgct gtaggcttcc tgctttccgc ggaagaatat tttgccgccg accatgccga
     4441 cgcagggccg gtatcccagc acgtttcttt cttcttccgc gcccgcgccg cagaccacgg
     4501 cgatgccgcc cgccatgaat tccgcgaaag aatcccccac tttgccgaag acccagagtt
     4561 cgggtggttc aaagcgcggg ttgaatttgg tcatggtcat gccgcgcgcg ccgatgtcgc
     4621 cgccgatgta aacccggccc tgagccatgg cgtttgccgt tccgttggtg gcatttccgt
     4681 gcacgatgat ttgcgcgcct gcgttgagcc agccgacgtc gtcggaggcc gggccgaaga
     4741 cttcaatcgt ggtgttcgcg aatcccatgg atcccaggcg ctggccggat gtgcccagga
     4801 catccacttt taccggcgcg ttttgcgcgc gccagaggcg gcctccgatg ccgtgctgcc
     4861 ccagcgccgt gacttcaatg tgacggcagc cgtcggccac ggccttctgc atgcgttctt
     4921 ccagaatccg cgaatccacg cgaatttggt tttcaacgcc ggagattttg atggtgtctt
     4981 ttttggtcat catcttgcct gtttaattcc ttctttgggg gaacggctgg aaccaccctg
     5041 cgggcggcaa gccgttccct acaaaacata ttgaatattc aacctttgcg ccgcatccgc
     5101 attgctgatg cccagtgcgt ccgacatgcc gatcggaagc gatgtcgatc gtcccagcgg
     5161 cgccacgatt tttttcagtt ccgtgtcaaa tgttaaatag acatccacca gccttcgggc
     5221 gaccttttcc ggatccaggc gccgatccag ccgcgggtcc tgcgaggtga tgcctttggg
     5281 gcaaaccccg atattgcaga cattgcagcg gtctccttca gatcccagac aaccggcggc
     5341 cgcctggagg atgtatttgc ccgtctgaac accgctggcg cccagcatga tcagggcggc
     5401 ggcnnnnnnn nnnggcggca ttggcggcca gattgccgtt tttcccgatg ccgccgccgg
     5461 caaagagagg aagctcgttt tgtttgccca gcttcaccag cgtcatgtag gcgtcgcgca
     5521 ggttgctggc gatggggtgt cccatgtggt ccatcgaaac attataggcc gcgcccgtgc
     5581 ccccgtcctc tccgtcgatg gcgagcccgg ccgcatatgg attgcgggtc aggttgttga
     5641 ggacggcctg cgtggtgctc gtggccgaga ttttcggata caccggcacg cggaatcccc
     5701 acgccatgga catggactgg atcatcttgg ccacggcttc ctcgatggag tacttggtct
     5761 gatgcgtggg cgggctgggc aggctgatgc cctgcggcac gccgcggatc gcggcgatca
     5821 gtttgttgac cttgtaccac atgagcagtc cgccgtcgcc gggcttcgcg ccctggccgt
     5881 acttgatttc gatggcgcag ggatcttcct tcattttggg cagggcgtgg atgatttcat
     5941 cccagccgaa atagccgctg gcgatctgca aaatgacgta tttcagaaag cgggactgaa
     6001 ggagccgggg cgggcagccg ccttcgccgg tgcagatgcg gaccggcatg cccatttcct
     6061 cgttcaggtg ggccacgccc atctgcaggc cctcccacat gttcggggac agggcgccga
     6121 acgacatgcc gccgatgatc agcggataga tttcgcggac gggcggcgtc cagccgttgt
     6181 cgcgttcggc agcgagcatc cgttcgggcg gcaggattcg gcccagcagc gtgtgcagct
     6241 cgaattcatg gcgtccggcg tccagcgccg ggtcggtcag catcgatatg cggacaaact
     6301 tgatccggtc gagtacgctt ttcgaatcgt ttcttcggcc gccccttctg cgggcttcgc
     6361 cgccctggtt gacgtgaaag cgcaggcggt cggattcatc cgtgcgcgcg ggaacgatgg
     6421 cgccgttggg gcagaccagc gtgcacatgc cgcagcccac gcaggcgtgc gccggatcgg
     6481 tgcgctgatc gatgccgtga tacacggaaa acagattggc gggccgctct tccagtccgg
     6541 gcggtatggt aagggcccgt ttgcggtgga cgcccagttc gatggcgcgc accggacaga
     6601 cggaggtgca ccggccgcac agggtgcagc ggtccttcct ccagagaatc tgccagggca
     6661 ggtctttggc gcttaatgta gagggggtaa tgcgttccac tggttccatt tttccatctc
     6721 ctgccgctcg cggcggacca gaacggtttc atatctcatc ggctgatggt ccagattgat
     6781 gtcccgttcg ggaatggacg cgtccagacc gcacatttcc gaggaaaacg cgaagatgcc
     6841 gggacgcccg cccaccacgc cggggcgcag cttcttggaa tcctgcacca tgaacatcga
     6901 cttgtccggc agacagccga tgacgcagtt gggc
//
LOCUS       DAOSIM010000100         6934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_9913_length_6934_cov_6.847943, whole genome shotgun sequence.
ACCESSION   DAOSIM010000100 DAOSIM010000000
VERSION     DAOSIM010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6934
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9913_length_6934_cov_6.847943"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1629)
                     /locus_tag="PLR47_05620"
     CDS             complement(<1..1629)
                     /locus_tag="PLR47_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOG12457.1"
                     /translation="MITKAKPTIEVSLEERLRLQKKLQDITNRIHAAQNIAQILIDLK
                     DGILNLFDAYSITLYVVDKPHNEIYSMFLAGSQLKEIRVPINNHSIAGYVANSFNIVN
                     IADAYNLAELKAIDPELTFDASWDKKSGFRTRQILAAPIFYKDQLMGVIQILNKKGGD
                     TKFTEEDIGLVMEIVEVLGVAFYNQQRFQRRRKTRFDFLLTRNLLKEEELEAAWGESR
                     EQKESIERYLMNKYKISKDDIGHSFEEFFRCRFIEYNDKTIIPGDLIANLKKDYLQRE
                     LWVPLEKIDGNIHVIVDDPNNIIKRDAIENLLKTKKVKYDVALSEDILKYINLFFSSS
                     DDEHSFTELLGKMDEESAGSEEEDGADIVAETDSVIMQLVNKIINDASTRRASDIHIE
                     PNVGKKNVEIRFRVDGDCAIYQTVPFNYRSAIISRVKIMSNLDITEKRKPQDGKIKFR
                     RSSGDELELRVATIPTQGGVEDIVMRLLAKSDTLLPLESMGLLKRNYDEMINIINKPY
                     GMILVVGPTGSGKTTTLHAALHVINRPDRKIWTAE"
     gene            complement(1728..3800)
                     /gene="glyS"
                     /locus_tag="PLR47_05625"
     CDS             complement(1728..3800)
                     /gene="glyS"
                     /locus_tag="PLR47_05625"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOG12458.1"
                     /translation="MSNELLFEIGTEEIPAGFLSKAVVDMEEMIRKALTEKRIAFESV
                     HCLATPRRLVLHIADVAPKQADQIVEKMGPARKAAFDENGRPTKAALGFARGQGLEIA
                     ELETITTDKGEYLCARKTIAGQPTADLLPDILKNFLVSIPFRKSMRWSAYDLRFARPI
                     HWLLALYGGKIVPLKVEDIESGNTSRGHRFMSPEAFAVDGFEDYLAKARKHFVIPDPA
                     ERKEFILEEAQKAAAEAGGKLFYTEDLLETVTFIVEYPVIIRGGFDPNYLQIPKEVLT
                     TTMIAHQKYFPVVDNAGKLLPCFIAVSNTRARNPEVVARGNERVLQARLADASFFFEE
                     DKKIPLESRVESLKKVVFHTLLGTSHKKVMRFRKLAVKIASKVKPSVKKNVDRAALLA
                     KADLESLMVGEFSELQGIMGREYALLAGEKPEIADAIYEHYLPIVAGGDLPRTDEGAI
                     VGIADKMDTIVGFFAVGMPPTGTADPYALRRQALGVINIILSRRYELGLNFLIDECLA
                     ALKDVLKKPAEDVKKDVLEFFRGRLQNQLISQSYAYDTVDAVLAADIDQLVLVIEKIQ
                     ALQAFRANPEFEPLSIAFKRIDNILKDFRGGSVDVNLLSQEAEIKLFANFENIGTRVE
                     KGIAEKDFTAALNRLAALRTPVDAFFESVMVMDNDEKIRFNRLSLLADISALFHKIAD
                     FSKIVTAG"
     gene            complement(3817..4689)
                     /gene="glyQ"
                     /locus_tag="PLR47_05630"
     CDS             complement(3817..4689)
                     /gene="glyQ"
                     /locus_tag="PLR47_05630"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOG12459.1"
                     /translation="MTFQELIFALEQYWHSQGCVIQQPYDMEVGAGTFHPATCLRALG
                     PEPWNVAYVQPSRRPTDGRYGENPNRLQHYYQYQVIMKPSPVNIQELYLNSLKSFGID
                     PLEHDIRFVEDDWESPTLGAWGLGWEVWLDGMEISQFTYFQQVGGFDCKPVCAELTYG
                     TERIAMYIQGINNVYDLQWTDKIKYGDVHHKGEVEFSTYNFEVADIPMLRRLFDMYEE
                     EALRIAEKNLPLPAYDYCLKCSHTFNLLNARGAISVAERTSYIGRVRNLARVSAELYM
                     KQREELGYPLLQNH"
     gene            5052..6209
                     /locus_tag="PLR47_05635"
     CDS             5052..6209
                     /locus_tag="PLR47_05635"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOG12460.1"
                     /translation="MLNFTFHNPTKVLFGKCTICQLAGEIPPGARVMVAFGAESARKY
                     GVLDEVLRALAGFDVTEFGGIEANPEYDTLCSGIALAREKKIDFLLAVGGGSVIDGTK
                     FMAAAIPFAGEPYQLMENKGAAIRQALPLGAVLTLPASGSEMNNRAIISRRAVNWKRA
                     AMNDWFFPRFAVLDPTKTCTLPPMQTANGVVDTFVHVLEQYLTYPVQGKVQDRLAEGL
                     LLLLIEEGPKALVEPENYDVRANLMWASTLGLNGLIGAGVPQDWAAHRAGYELTVLYG
                     LDHARTLAVLVPAMLQVRAGGKKEKLLQYGERVWGISSGAEDVRITAAIEKTKAFFGS
                     MGLPTSLSGCGIEKVDADRFIELLEEHGLTAMGERGEVTPAMMREILALCA"
     gene            6226..>6934
                     /locus_tag="PLR47_05640"
     CDS             6226..>6934
                     /locus_tag="PLR47_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOG12461.1"
                     /translation="MHSILPEIGIAVLAATAMGFLFQLCRQPVILGYLVAGALIGPQI
                     GFSLVSHPDNIEVISEIGLILLLFIIGLELNPAKLLSSGKKLIYAGVGQFIFCVLIGL
                     GFFVLLGYPLGGGRIEALYLSLFCALSSTAIVVKLLYDKFELDTLPGRISLGILIFQD
                     LWAILILALQPNFMDPKLHLVALALGKGVLLLAVGFLLSRFALRWIFAKIAKTPEMVV
                     ATSIAWCAFMAGLGSWIG"
BASE COUNT         1339 a         1855 c         2078 g         1662 t
ORIGIN      
        1 ttcagcggtc cagattttcc ggtccggccg gttgatgacg tggagcgccg cgtgcagtgt
       61 ggtggttttg ccggagccgg tcgggccgac gaccaggatc atgccgtagg gcttgttgat
      121 gatgttgatc atttcatcat aattgcgttt gagcagcccc atggattcca gcggaagcag
      181 ggtgtcgctt ttggccagaa gccgcatgac aatgtcttcc acgccgccct gcgtgggaat
      241 ggtggcgacg cgaagctcca gttcatcccc ggaagagcgc cgaaacttga ttttgccgtc
      301 ctgtggtttt cttttctccg tgatatccag gttggacatg atcttgacgc gcgagatgat
      361 ggcggaccgg tagttgaagg ggacggtctg gtaaatcgcg cagtcgccgt cgacgcggaa
      421 ccggatttcc acgtttttct tgccgacgtt gggttcgatg tgaatgtcgg acgcccggcg
      481 ggtggaggcg tcgttgatga ttttattgac gagctgcatg atcacgctgt cggtttccgc
      541 gacgatgtct gctccatcct cctcttcaga gcccgcgctt tcttcatcca tcttgcccag
      601 gagctccgta aaggaatgct cgtcgtcgct ggagctgaag aacaggttga tgtatttcag
      661 gatgtcttcc gacagagcga cgtcatactt cactttcttg gttttcagaa gattttcgat
      721 ggcgtctctt ttaatgatgt tgttcggatc atcgacaatc acgtggatgt tgccgtcgat
      781 tttttccagc ggcacccaca gttcgcgctg gaggtagtct ttcttcaggt tggcgatcaa
      841 atcgccgggg atgatggttt tatcgttgta ttcaatgaac cggcagcgga aaaactcttc
      901 gaaggagtgg ccgatatcgt ctttggagat tttgtacttg ttcatcaggt accgctcgat
      961 ggattctttc tgctcccgcg actcccccca ggcggcttcc agttcttctt ccttgagcag
     1021 gttgcgggtc agcagaaaat cgaaccgcgt tttgcggcgg cgctggaagc gctgctggtt
     1081 gtaaaaggca acgcccagaa cctcgacgat ctccatgacc agtccgatgt cttcctcggt
     1141 aaatttggtg tctccccctt ttttgttgag aatttgaatc acgcccatga gctgatcttt
     1201 atagaaaatc ggggcggcga gaatctgcct ggttcggaat ccggactttt tgtcccagct
     1261 tgcgtcaaag gtcagttcgg gatcgatggc cttgagttcc gccaggttgt atgcgtccgc
     1321 gatgttgacg atgttgaagg agttggccac gtagccggcg atgctgtgat tgttgatcgg
     1381 aacgcggatc tccttgagct gagatcccgc cagaaacatg gaatagattt cgttgtgcgg
     1441 cttatcgacg acgtacagcg tgatggagta ggcgtcaaac agattcagga tgccgtcttt
     1501 caggtcgatc aggatttgcg caatgttctg cgcggcgtgg atgcggttgg tgatgtcctg
     1561 aagctttttc tgcagccgca gcctttcctc cagactcacc tcgatcgtgg gtttcgcctt
     1621 cgtgatcatc cggttatctc tccattttcg ttttattcat aaaaatcgcg tacatcctag
     1681 caagccgcgc ggggaatgtc aaccaaacga atttggcgcc gggcggatca gccggcggtg
     1741 acgattttcg agaaatcggc gattttatgg aacagtgctg agatgtccgc cagcagcgac
     1801 aggcggttga agcggatttt ctcgtcgttg tccatgacca tgacggactc gaagaaagcg
     1861 tccacggggg tgcgcagagc ggccagccgg ttcagcgccg cggtgaaatc cttctcggcg
     1921 atgcctttct ccacgcgcgt tccgatgttt tcaaaattcg cgaacagctt gatctccgct
     1981 tcctgggaaa gcaggttgac gtccacggaa ccgccgcgga aatccttcag gatgttgtcg
     2041 atccgcttga atgcgatgga gaggggttcg aactccggat tggcccggaa agcctgcagc
     2101 gcctgaatct tttcgatcac cagaacaagc tggtcgatgt ccgcggccag gaccgcgtcc
     2161 accgtgtcgt aggcatagct ctggctgatg agctggttct gcagacgccc ccggaaaaat
     2221 tccagaacat cttttttgac gtcttcggcc ggcttcttca ggacgtcctt cagagccgcc
     2281 aggcattcgt cgatcagaaa gttcaggccg agctcgtagc gccgcgacag aatgatattg
     2341 atgacgccga gcgcctggcg acgaagggca taggggtcgg ccgtgccggt ggggggcatg
     2401 cccacggcga agaagccgac aatggtgtcc attttatcgg caatgccgac aatggcgcct
     2461 tcatccgtcc ggggcagatc gcctccggcg acaatcggca ggtaatgctc gtaaatggcg
     2521 tcggcgattt ccggtttctc gccggccaga agcgcgtatt cccgtcccat gatgccctgg
     2581 agttcggaaa attcgcccac catgagcgat tccagatcgg ccttggccag aagcgcggcg
     2641 cggtccacgt ttttcttgac ggagggcttc actttggaag caattttgac cgccagcttg
     2701 cggaagcgca tcaccttttt atgggaggtg cccagcagcg tgtggaacac gacttttttc
     2761 agggactcca cacggctctc cagcgggatt ttcttgtcct cctcgaaaaa gaacgaggcg
     2821 tcggccagcc gggcctgcag tacgcgctca ttgccccggg cgaccacttc gggattccgg
     2881 gcgcgggtgt tgctgacggc gatgaaacag ggcagaagct ttcccgcgtt gtccacgact
     2941 gggaagtact tctggtgcgc gatcatggtg gtggtcagca cttccttggg gatctgcagg
     3001 taattcggat cgaagccgcc gcgaatgatc accgggtatt ccacgataaa agtgacggtt
     3061 tccagcaggt cttccgtata gaaaagcttt cccccggctt cggcggccgc tttttgcgct
     3121 tcttcgagaa tgaattcttt tctctccgcg ggatcgggga tgacaaaatg ctttcgggct
     3181 ttggcgaggt aatcttcgaa tccgtcgacc gcaaaagcct ccgggctcat gaagcgatgg
     3241 ccgcgcgagg tgttgccgct ttcaatgtct tccaccttca gcggaacgat tttgccgccg
     3301 tacaacgcca gcagccagtg gatcggccgg gcaaagcgca gatcgtaagc cgaccagcgc
     3361 atggatttgc gaaacgggat ggacacaaga aagtttttga ggatgtccgg cagcaggtct
     3421 gcggtcggct gtccggcaat ggtttttcgc gcgcacagat actcgccctt gtcggtcgtg
     3481 atggtttcca gttcggcgat ctccagaccc tgtccccggg caaaccccag cgcggccttg
     3541 gtcggccggc cgttttcgtc aaaggccgcc tttcgggccg gccccatttt ttcgacgatc
     3601 tgatcggcct gcttcggcgc gacgtccgca atgtgcagca ccagccggcg gggagtggcc
     3661 aggcagtgga cggactcgaa ggcgattcgt ttttcggtaa gcgccttgcg gatcatttct
     3721 tccatgtcca cgaccgcttt ggataaaaac cccgcgggga tttcttcggt gccgatttca
     3781 aaaagaagtt cgttgctcat cgtgtctcca gtttttctag tgattctgca aaagagggta
     3841 tcccagttct tcccgctgct tcatgtagag ttccgcgctc accctggcca ggtttcgcac
     3901 ccggccgatg tagctggtgc gctccgcgac gctgatcgcg ccgcgggcat tgagcaggtt
     3961 gaacgtgtgc gagcatttca ggcagtagtc ataggcggga agcggaagat ttttctccgc
     4021 gatgcgcagc gcctcctctt catacatatc aaacagcctg cgcagcatcg gaatgtcggc
     4081 cacttcaaag ttatacgtgg aaaattcgac ttcgccctta tggtgcacgt cgccgtattt
     4141 gattttatcc gtccattgca ggtcatagac gttgttgatg ccctggatgt acatggcgat
     4201 gcgctccgtt ccgtaggtca gctcggcgca gaccggcttg cagtcgaagc cgcccacctg
     4261 ctggaaatac gtgaactgcg agatttccat gccgtcgagc cacacctccc agcccagccc
     4321 ccaggcgccc agcgtcgggg attcccagtc gtcctcgacg aaacggatat cgtgctccag
     4381 cggatcgatg ccgaagcttt tgagcgaatt gaggtaaagc tcctggatgt tgaccggcga
     4441 cggcttcatg atcacctggt actggtaata atgctgcagg cggttgggat tttcgccgta
     4501 gcgtccgtcg gtcggccgcc gtgaaggctg tacgtaggcc acgttccagg gttccgggcc
     4561 cagcgcccgc aggcaggtgg cgggatgaaa cgttcccgcg ccgacctcca tgtcataagg
     4621 ctgctgaatg acgcagccct gggagtgcca gtattgctcc agcgcgaaga ttaattcctg
     4681 aaaagtcaca atgtcctcct tggggaatgc cggcaggaac tcaggaccgg tgatgcgggt
     4741 ccgagacatt acttctgcca gcggctacct agcatgaaag gccagggggg tcaagatacg
     4801 ggaaggggga agattggtgg atagtggata gtggatggtg gatagaaaaa cagtaatgag
     4861 tgacgaacaa tgagcgatga gtggaaagtg aaaagatgtc atggtgagct tgtcgaacca
     4921 tgggcaatga gtcatgggga atagggcatg ggtcattttt ctgttgacag gccggggagt
     4981 cttttctatg aacaacttca gtgattggag gcgtttgtca aaagcgtttc tttactccgg
     5041 tggagctgaa tatgctcaat tttacctttc acaatccgac gaaagttctt ttcggcaaat
     5101 gcacgatttg tcaactggcg ggtgaaattc cgcccggcgc cagggtgatg gttgccttcg
     5161 gcgctgaaag cgccagaaaa tacggcgtcc tggatgaagt tctcagggcg ctggcgggat
     5221 ttgacgtgac tgaattcggc ggcatcgaag cgaatccgga atatgatacg ctttgctccg
     5281 gcattgctct ggcccgcgaa aaaaagatcg atttcctcct cgcggtgggc ggaggctccg
     5341 tcattgacgg aaccaagttc atggccgccg ccattccctt cgccggcgaa ccgtatcaac
     5401 tgatggaaaa caaaggcgcg gcgatccgcc aggccctgcc gctgggcgcg gtcctgacgc
     5461 tgccggcgag cggttcggaa atgaacaacc gggccattat ttctcgccgg gctgtaaact
     5521 ggaaacgcgc cgccatgaac gactggtttt tccctcgttt tgccgttctg gacccgacaa
     5581 agacctgcac gcttccgccg atgcaaaccg cgaacggcgt ggtcgatacg tttgtccatg
     5641 ttctggagca gtatctgact tatcccgtgc agggaaaggt tcaggaccgc cttgccgaag
     5701 ggttgctgct tctgctgatt gaagaagggc cgaaagcgct ggtggaaccg gaaaattacg
     5761 atgtgcgcgc caacctgatg tgggcctcga cgctggggct gaacggtctg atcggcgccg
     5821 gcgttcctca ggactgggcg gcgcaccggg caggctatga attgacggtg ctgtatggac
     5881 tggaccatgc gcggacgctg gccgtgctgg tgccggccat gctgcaggtc cgggccggcg
     5941 gaaaaaagga aaagctgctt cagtatggcg agcgtgtctg gggaatatcc tccggggccg
     6001 aagacgttcg gatcacagcc gcgattgaaa agacaaaggc tttttttgga agcatgggcc
     6061 tgccgacgag cctgtcggga tgcggcatcg aaaaagtgga tgccgaccgg ttcattgaac
     6121 tgcttgagga gcacggcctc accgccatgg gcgagcgggg cgaggttact cccgcgatga
     6181 tgcgggagat tctggcgctt tgcgcgtaaa ggaaggaatt taaggatgca cagcattctt
     6241 cctgaaatcg gcattgccgt tctggcggcg acggcgatgg gttttctctt ccagctatgc
     6301 cgccagccgg tcattctggg ctatctggtt gccggagcgc tgatcggacc gcagatcgga
     6361 ttttcgctcg tttcccatcc cgacaacatc gaagtcatct ccgagatcgg cctgatcctt
     6421 ctgctgttta tcatcggcct ggagctcaat cccgccaaac ttctttcctc tggaaaaaaa
     6481 ctgatttatg ccggcgtggg ccagttcatt ttctgcgttc tgatcggttt gggatttttt
     6541 gtgctgctgg ggtatcccct gggcggcgga cgaatcgaag cgctttacct ctcgctgttc
     6601 tgcgctcttt ccagcacggc catcgtcgtg aagctgctct acgacaagtt cgagctggac
     6661 acgctgcccg gccgcatcag cctgggcatt cttatcttcc aggacctgtg ggccattctg
     6721 attctggcgc tgcagccgaa tttcatggat ccgaagctgc acctggtcgc gctggcgctg
     6781 ggcaaaggcg tcctgcttct ggccgtcggc tttttgctca gccgattcgc gctgcgctgg
     6841 atttttgcca aaatcgccaa aactccggaa atggtggtcg ccacgtccat cgcctggtgc
     6901 gcctttatgg ccggactggg ctcctggatc ggca
//
LOCUS       DAOSIM010000101         6923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_9936_length_6923_cov_7.424432, whole genome shotgun sequence.
ACCESSION   DAOSIM010000101 DAOSIM010000000
VERSION     DAOSIM010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9936_length_6923_cov_7.424432"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLR47_05645"
     CDS             <1..239
                     /locus_tag="PLR47_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12462.1"
                     /translation="WEFNFKADAFYFQSLRMPTADWPETQEDTEGSLLERIYRIENAV
                     KTIDQLYESFLAIRFSPEWAKNEKPRLATWLRKE"
     gene            353..643
                     /locus_tag="PLR47_05650"
     CDS             353..643
                     /locus_tag="PLR47_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12463.1"
                     /translation="MDQKQIFKQMMDFNKQAFDNSFSVMSELQEQTEKLMLNFLEKAE
                     WIPEDGKKAMQDWIVNNKKSRNDFKAAADDSYRKVAELFEQAEKQQTPKKKK"
     gene            670..933
                     /locus_tag="PLR47_05655"
     CDS             670..933
                     /locus_tag="PLR47_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12464.1"
                     /translation="MDPKQVAKQMIVFNKTAFDNNFRAMQALQEQTERLVGRFWEKSP
                     MFPEEGKKAISDWMSAYKKGCDDFKSLVDDNFKKVEDFFKETK"
     gene            971..2545
                     /locus_tag="PLR47_05660"
     CDS             971..2545
                     /locus_tag="PLR47_05660"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG12465.1"
                     /translation="MDHPHIVERMTRPDFYPHRPQKVEAVQTHISYVFIAGDYVYKVK
                     KPVNFGFLDFTTLEKRKFYCEEELRLNRRLAPSIYLDVAPIGRDDAGRLTLGKTANIV
                     EYAVLMNKLPLDKMLKILLARGKADAGIMDAVAKKIARFHQEAQTGGRIDEMGSIGVI
                     RHNHEENFAQTEKYIDVTLSAFQHGFLKSYAEKFLAASEALLAKRVAEHKIRDCHGDL
                     HLEHICVADEIIVFDCIEFNERFRFADVAAEVAFLNMDLDYNGYFSQSADFTNSYLKY
                     SHDEDLRALLNFYRCYYAFVRGKVTSFRLDQKELPQAEREEIRRIASKYFDLACAYAS
                     RLEKPALILTAGLIGSGKSYQARNLADRFGTEVIRSDVLRKELLRIAPTEKHHEAFGQ
                     GIYSGDITRKTYEKAVELAAEKIGRGIPVILDASFRCRADRTMAADMARRLGVPFYVI
                     ECTCPDDVVKTRLEKRMQDRDNPSDGRWEILEEQKKQYEAVTEIPRSCLFHIDTSDNP
                     ENVRRQIVSSIKMAEL"
     gene            complement(2689..3471)
                     /locus_tag="PLR47_05665"
     CDS             complement(2689..3471)
                     /locus_tag="PLR47_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOG12466.1"
                     /translation="MKLRHASVILAMVLICTGCSFNMAAIKSFFVKSDPVRSTPEGLY
                     SRGTADYQNGSYKKAREYFTRLKEEYPLHNLAIMAELGIADSFYSVKEYGEAELAYRD
                     FVSLYPTNENIPYALYQTGMCHFVQIEAIDRDQTETIKARREFERLIARYPQSKFSIL
                     AEKMVRECKLKLAEHEFYVGNFYFKNKKYNAALKRFEGIARDYAGVGLDLKVEAYIAE
                     TRDRLAKEEREEKLKEDKEKAKAAKKEEKEKNKIKEKEPTNS"
     gene            complement(3531..3860)
                     /gene="trxA"
                     /locus_tag="PLR47_05670"
     CDS             complement(3531..3860)
                     /gene="trxA"
                     /locus_tag="PLR47_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOG12467.1"
                     /translation="MSGQFLGVATDANFEQEVLKSDKPVLIDFWAPWCGPCKAIGPIV
                     EEIAGEYSDRVKVMKLNVDESQKSAVAFGVRSIPTLILFKDGKVLDTLVGMTPKEKLE
                     EFVKKSL"
     gene            4011..4724
                     /locus_tag="PLR47_05675"
     CDS             4011..4724
                     /locus_tag="PLR47_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HOG12468.1"
                     /translation="MKRLGVLSGTFMLEKSDWIRKGKRQRIHNVYGEAAAIETDSIVF
                     ILRHGDDPNDYILPHRINHHANLQALQDLNVTEVIGINSTGSLKTKLRPGMIVIPDDF
                     ITFAATPSIYHNKAVHITPSLNEAVRQKLITAAHDCNLDVVETGTYWQVAGPRLETRA
                     EIRMMSHFADVVGMTMAHEAVAALELGLPYASACSIDNLGNGLLEKPLSLEEIIAGTR
                     KNADLMIRLLEEYIEGVDK"
     gene            complement(4765..5715)
                     /locus_tag="PLR47_05680"
     CDS             complement(4765..5715)
                     /locus_tag="PLR47_05680"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOG12469.1"
                     /translation="METRRDFIKKSVVVCAALSLPLPWAANAQTVQREFKTRDPKRAL
                     VVSYSQSGFSARYGKLIACLLKDQGLTVEWADMRRFDPKRLPDYDLILVGSPVFYYDI
                     PENVADWLSSAPKLTGIPVAAFVSFGGPEGNQHNALCHALHLLAEKEAVPVGMEAFRS
                     IPAYPASWDSANQRSGEHLPDEATYNRVREFAKQIQERVRQGQSLAYSSEIALREIVR
                     MLPLVWLNKKAVGKHTVDGAKCIGCRTCVKMCPVDAIHPERQFVDHDKCIACFGCLNN
                     CPADAVVVEYRGNRLYGFPEYLKRRKITILEPPEFQHCKL"
     gene            complement(5747..>6923)
                     /locus_tag="PLR47_05685"
     CDS             complement(5747..>6923)
                     /locus_tag="PLR47_05685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG12470.1"
                     /translation="DLGVHTETFTDGLIDLIESGVVTCRKKSLHPGKIVASFCMGTRR
                     LYDYVHNNPLFEFRPCQYVNDPYIIARNDRMVSINSALTVDLTGQVCSDSLGFEFYSG
                     IGGQVDFVRGSAMSQRGKSIMVLPSTTEDGAISRIVPYPSAGSGVVVTRGDIHYVVTE
                     YGIAYVHGKSIRDRAMMLINIAHPKFRDELLEAAKRQGYIYRDQTLPVVLYPKEYETN
                     WVDKKGTSLFFRPVKATDERAIQELIYELPPQDVYTRFFHNLKSFSHKVAMPMAAIDY
                     DDKMAIVAVIGKEEPEGREKIVAIGNYANNPNTRYAEVAFSTHQDWQDRGIGTFLLQY
                     LIRIAKGKNIEGFTADVLSRNLPMMHVFSKCGYPMTTHLDMGVYELKINFGGEEKKE"
BASE COUNT         1636 a         1923 c         1833 g         1531 t
ORIGIN      
        1 aatgggaatt caatttcaag gccgacgcct tttacttcca gtcgctcaga atgccaaccg
       61 cggactggcc ggaaacacag gaagacacgg aaggaagcct tctggagcga atctaccgaa
      121 ttgaaaacgc ggtcaaaacc atcgaccagc tctatgaatc ctttctggcg attcgcttct
      181 ccccggagtg ggcgaaaaac gaaaaacccc gtctggcgac ttggttgagg aaggaatagc
      241 tcatcactca ttgctgtctg taaaaattac tttacaaata agattaaatc gttagaatag
      301 gagcccgttt caatccttgg aacagacaaa acaaagagga ggtgaagcgc ttatggatca
      361 aaaacagatc ttcaaacaaa tgatggattt caacaagcag gcgtttgaca atagtttcag
      421 cgtgatgtcg gagctgcagg aacaaacgga aaaactgatg ttgaattttc ttgaaaaggc
      481 ggaatggatt cccgaagacg gcaaaaaagc catgcaggac tggatcgtaa acaacaagaa
      541 aagccggaat gatttcaagg cagccgcgga cgacagctac agaaaggtcg ccgaattatt
      601 cgaacaggcg gaaaaacagc agacaccgaa aaagaaaaaa taaacctttt tttcagcaag
      661 gaggtggcca tggacccgaa gcaagttgcc aaacagatga ttgttttcaa caagacggct
      721 tttgacaaca atttccgagc catgcaggcg ctgcaggaac agacggaaag actggtcggc
      781 agattctggg aaaaatcacc gatgtttccg gaagaaggaa aaaaggcgat ctccgactgg
      841 atgtccgcct ataaaaaagg ctgcgacgat ttcaaaagcc tcgtggacga caactttaaa
      901 aaagtggaag actttttcaa agaaaccaaa tagtccacct gcggcgggtc tgttttttca
      961 cgaggtattc atggatcacc cccacattgt cgaacggatg acccgtcccg atttttatcc
     1021 gcaccggccc caaaaggtcg aagcggtgca gacgcacatt tcgtatgtct tcatcgccgg
     1081 cgactacgtg tataaagtta aaaaaccggt caatttcggg ttcctcgact tcacgacgct
     1141 cgagaagcga aaattctact gcgaagaaga gctgcggctg aacaggcgcc tggcgccgag
     1201 catttacctg gacgtcgcgc ccatcgggcg ggacgacgcg gggcggctga cgctcggcaa
     1261 aaccgcgaac atcgtggaat acgccgtttt gatgaacaag ctgcccctgg ataaaatgct
     1321 aaaaatcctg ctggcccggg gcaaagccga cgccggcatc atggatgccg tggcaaaaaa
     1381 aatcgcccgg tttcatcagg aagcccagac gggcggccgc atcgacgaga tgggctccat
     1441 cggcgtgatc cgccacaacc acgaagaaaa cttcgcgcaa accgaaaaat acatcgacgt
     1501 caccctgtcc gcctttcaac acggctttct gaaatcctat gcggaaaaat ttctcgccgc
     1561 cagcgaagct ctgctggcca aacgcgtcgc ggagcacaaa atccgggact gccacggcga
     1621 tttgcacctg gagcacatct gcgtggctga tgaaatcatc gtttttgact gcattgaatt
     1681 caacgaacga ttccggttcg ccgacgtggc ggccgaggtc gcctttctga acatggacct
     1741 ggattacaac ggttatttca gccagtccgc ggacttcacg aactcgtatc tgaaatactc
     1801 ccacgacgaa gacctgcgcg ccctgttgaa tttttaccgc tgctattacg ccttcgtccg
     1861 gggcaaagtc accagcttcc gcctggatca gaaagagctg ccgcaagccg aacgcgagga
     1921 gatccgccgc atcgcctcaa aatactttga cctcgcctgc gcatacgcgt cgcggctgga
     1981 aaaaccggcc ttgattctga ccgccggact gatcggctcc ggcaagagct accaggcgcg
     2041 caatctggcg gatcgattcg gcacggaggt gatccggtcg gacgttctgc gcaaggagct
     2101 tttacggatt gcccccaccg aaaagcatca tgaagctttc ggccagggca tctattcagg
     2161 ggacattacc cggaagacct acgaaaaagc cgttgaactg gccgccgaaa agatcgggcg
     2221 ggggattccg gtgattctcg acgcctcctt tcgatgccgc gcagaccgga cgatggccgc
     2281 ggacatggcg cgaaggctcg gagtcccgtt ttatgtgatt gaatgcacct gccccgacga
     2341 cgtagtgaaa acccgtctgg aaaaaagaat gcaggaccgg gacaatcctt ccgacggccg
     2401 ctgggaaatc cttgaggagc agaaaaaaca atacgaagcc gtcacggaaa ttccccggag
     2461 ctgtctcttt cacatcgaca cgtcggacaa cccggaaaat gtgcgccggc aaattgtttc
     2521 ctcgatcaaa atggcggaac tttaaggcct gtcgcccaaa tatccttttc gcttgggcaa
     2581 caaccgactg accgtgtgat ctccggacag gcaggtgcgg cgggaacggc ttgtttaaaa
     2641 cgactgcttt gcgcgtgcgg tgattcatca cgcgaaaaaa aggcgggctc aggagtttgt
     2701 cggttctttc tctttgattt tgttcttttc tttttcttct ttcttggcgg ccttggcctt
     2761 ctctttgtct tccttcaact tctcttcccg ctcttctttg gcgaggcgat cccgggtctc
     2821 cgcaatgtag gcctccactt tcaggtccag ccccacgccg gcgtaatcgc gggctatgcc
     2881 ctcaaaacgc ttcagagcgg cattgtattt tttgtttttg aaataaaagt tgccgacgta
     2941 aaactcgtgc tcggcgagtt tcaacttgca ctcccggacc atcttttcgg ccagaatgga
     3001 aaatttgctc tgcggatagc gggcgatcag ccgctcgaac tcccggcggg ccttgatggt
     3061 ttccgtctgg tcgcggtcaa tcgcttcaat ctgcacaaag tggcacatcc cggtctggta
     3121 aagcgcgtag ggaatgtttt cgttggttgg gtacagagac acaaaatcac ggtaggccag
     3181 ctccgcctca ccgtattcct tgacgctgta aaaagaatcc gcgatgccca gttcggccat
     3241 aatcgccaga ttgtgcagcg gatattcctc tttgagtcgg gtaaagtatt ccctggcctt
     3301 tttgtaggag ccgttctggt aatccgccgt gccccgggaa tagaggcctt cgggggttga
     3361 ccggaccgga tcgctcttca caaagaacga cttgatggct gccatgttga aagagcagcc
     3421 ggtacagatc agcaccatgg ccagaatgac gcttgcgtgt cttaacttca cagggatacc
     3481 cgttccatca ggaatgaaat ttttgcttcc gtttgctttg cgtaatgcct tcacaggctt
     3541 ttcttcacaa actcttcgag cttttccttc ggcgtcatgc cgaccagcgt gtccagcact
     3601 ttcccgtctt taaacaggat gagagtcgga atgctgcgga cgccgaaggc gacggccgac
     3661 ttctggcttt catccacatt cagtttcatg acctttaccc ggtccgaata ttcgccggcg
     3721 atttcttcca caataggacc gatggccttg cacggtccgc accagggcgc ccagaaatct
     3781 atcagaaccg gcttgtcgct tttcaggact tcctgctcga aatttgcatc cgtcgccaca
     3841 cccagaaatt ggccgctcat gattcgcaac ctccttttct atttgggagt tatatgtcac
     3901 agcgtgtttc aaaaagtcaa atgctatttg accgcctttc ccgcaggcgt gattcttttg
     3961 acgcccgccg gtttttctgc tatgaaatgg agcacatcgg gaggttagtc atgaaacgtt
     4021 tgggcgtctt atcagggaca ttcatgctgg agaaaagcga ctggatccgg aaaggcaaaa
     4081 ggcaacggat ccacaacgtt tacggagagg cggcggcgat cgaaacggat tccatcgtct
     4141 tcattctcag gcacggcgac gatcccaacg actatattct gccccaccgg atcaaccacc
     4201 acgccaatct ccaggcgctg caagacctta acgtcaccga agtgatcggc atcaactcta
     4261 ccgggtccct gaaaacaaaa cttcgtccgg ggatgattgt cattcccgac gatttcatca
     4321 cctttgcggc aacgccgtcc atttatcata acaaagccgt ccatatcaca ccctctttaa
     4381 atgaggccgt acgccaaaaa ctgattacag cggcacacga ctgcaatctg gatgtcgtgg
     4441 agacggggac ttactggcag gttgcgggcc cccgcctgga aacccgggcg gagatccgga
     4501 tgatgtccca ttttgccgat gtcgtcggca tgacgatggc ccatgaagcg gttgccgcgc
     4561 tggagttggg gctcccctac gcctcggcct gctccatcga caatttaggc aacggcctgc
     4621 tggaaaagcc tttgagcctg gaggaaatca tcgccggaac acgcaaaaac gcggacctga
     4681 tgatccgcct gctcgaggag tatattgagg gggtcgacaa ataggctatt tacaataata
     4741 tcaattattt atagcttcac gggttcaaag tttgcaatgc tggaactccg gcggctccag
     4801 gatggtgatc tttctgcgct tgaggtactc cggaaaaccg taaagccgat tgccgcgata
     4861 ctcgacgacc acggcatccg ccggacagtt gttcaggcag ccgaagcagg caatacactt
     4921 atcatgatcc acaaactgcc tctccggatg aatcgcgtcc accggacaca ttttgacgca
     4981 agtccggcag ccgatgcatt tggcgccgtc caccgtatgt ttgccgacgg cttttttgtt
     5041 gagccagacc aaaggaagca tccgcacgat ttcccgaagc gcaatttcgg aggaataggc
     5101 aagggattga ccctgccgca ctctttcctg aatctgtttg gcgaattccc gaacccggtt
     5161 gtacgtcgcc tcgtcgggca gatgctcccc ggagcgctga ttggcgctgt cccaactggc
     5221 aggataggcc ggaatgctgc ggaaggcctc catgcccacg ggcacggctt ccttttccgc
     5281 cagcagatga agcgcgtggc agagtgcgtt gtgctgattg ccttccggcc cgccgaagga
     5341 cacaaacgcc gccacgggaa ttcccgtcag cttcggcgcg gaagacagcc agtccgccac
     5401 gttttccgga atatcgtaat agaaaaccgg agagccgacc agaatcaaat cgtaatccgg
     5461 caggcgcttc ggatcgaaac ggcgcatgtc ggcccattca accgtcaatc cctgatcctt
     5521 cagaagacag gcgatcagtt ttccgtagcg cgcggagaat cccgactggc tgtagctcac
     5581 aaccagcgcg cgtttcggat cgcgggtttt gaattcccgt tggacggtct gtgcgttcgc
     5641 ggcccacggc agcggcaggc ttaaagcggc gcagaccacc acgctttttt tgatgaagtc
     5701 tcttctggtt tccatggaat cggcccggcc tttccgcggc gcctgattat tctttctttt
     5761 cttcgccgcc gaaattgatt ttcaactcat agactcccat gtccagatgc gtggtcatcg
     5821 gatagccgca cttggagaaa acatgcatca tcggcaggtt gcgcgacaaa acgtccgccg
     5881 tgaagccttc gatattcttc cccttcgcga tgcggatcaa atactgcagg aggaaggtgc
     5941 cgatgccccg gtcctgccag tcctggtgcg tggagaaggc cacttcggcg tagcgtgtgt
     6001 tcgggttgtt ggcgtagttc ccgatggcca cgatcttttc tctcccttcc ggctcttcct
     6061 tgccgatgac cgccacgatg gccatcttgt cgtcgtaatc gatcgccgcc atcggcatgg
     6121 ccaccttgtg ggaaaaggac ttcaagttgt gaaagaaccg cgtataaaca tcctgcggcg
     6181 gcagttcgta gatcaattcc tgaatggcgc gctcatccgt cgccttgacc ggacggaaaa
     6241 acagcgaggt ccccttcttg tccacccagt tcgtttcata ctccttcggg tacagcacca
     6301 ccggcagtgt ctggtcccgg taaatgtagc cctggcgctt ggcggcttcc aggagttcgt
     6361 cgcgaaactt cggatgggcg atgttgatga gcatcatggc ccggtcgcga atggattttc
     6421 cgtgcacata ggcgatgccg tattccgtca cgacgtaatg aatgtccccg cgcgtcacca
     6481 ccacgccgct gcccgctgaa ggatagggca cgatgcggga aatggcgccg tcttccgtgg
     6541 tcgaaggcag caccatgatg gatttgcccc gctgcgacat ggccgaaccg cggacaaaat
     6601 ccacctggcc gccgatgccg ctgtaaaact caaacccgag ggaatccgaa cagacctgcc
     6661 cggtgagatc gacggtcagc gcggaattga tggacaccat ccggtcgttc cgggcgatga
     6721 tatacggatc gttgacgtac tggcacggac ggaattcaaa gagcggattg ttgtgaacat
     6781 agtcatacag gcgccgcgtg cccatgcaga aggacgcgac gatcttgccc ggatgcagcg
     6841 attttttccg gcaggtcacc acgccgctct caatcagatc gatgaggccg tcggtaaacg
     6901 tttccgtgtg cacgccgagg tct
//
LOCUS       DAOSIM010000102         6792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_10172_length_6792_cov_6.966751, whole genome shotgun sequence.
ACCESSION   DAOSIM010000102 DAOSIM010000000
VERSION     DAOSIM010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10172_length_6792_cov_6.966751"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PLR47_05690"
     CDS             complement(<1..706)
                     /locus_tag="PLR47_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12471.1"
                     /translation="MKHAVKIMALFLCFFVPLMGQVAFAETRTFIKEYTYQAGDEDSK
                     NSSRVIALREVKRLLLEELGTYLESETEVKNFQLTKDQITTLTAGIVQTEIIAEKWDG
                     HVYWLKSKIVADSDKVVQSIDAMRKDRDKTKELESMKQKSDELLREVERLRKAMASAK
                     NGGRESQKAAYDQSIRELSAADWIEKGHAVSGPEDNFKGAFDAYSRAIELDPKNIEAY
                     YFRARISEKNQAMSDYY"
     gene            complement(750..3407)
                     /gene="polA"
                     /locus_tag="PLR47_05695"
     CDS             complement(750..3407)
                     /gene="polA"
                     /locus_tag="PLR47_05695"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOG12472.1"
                     /translation="MAEKNQTPLLAIVDGSNYLYRAYYGIPALTNSKGFPTNAIYGFT
                     TMLMKLLRDLSPDYIVVTFDLKGPTTRHGEFEDYKATRKPMPDDLIPQVPFIKDVIRG
                     FSICVLEKQGTEADDLIGTLAVAARQKGWRTVMISGDKDLMQLIDDRVTMVDTMKDKT
                     YDAAAVEEKFGVGPEKVVEILGLTGDTSDNIPGVPGIGPKTARRLIEEYGSVDAVIEN
                     AQKLHNVKLRESFRNYADQARLSRQLALIRTDIEMDFHLEDAARRKPDLETLARLFGE
                     FEFSSLLQQIKIKAEKPVKACKVVADGKSLSELIAGLHNNSKIACDMIREKNPPQELI
                     GMAFRAGGDSFYLPLGHTPAKEQLDPKETFSMLASFFANAEIVKYAYDLKTTLVSMPD
                     LTIRAGFDLQLAAYLLNPARHSYALDEMAWEYLHERISTPDDIAGGKGKTYALSLAPV
                     DKMAAYAGERAEAIMALAEVLESRLEEAGAASLYRDVEMPLLFTLAGMEKKGVLVDTV
                     LLQQMSDELGQLLSISEDKIHRLAGEKFNINSPKQLQTILFDKLKLPKGKKIKEGYST
                     DVDVLTDLARSHELPAEILAYRSLSKLKSTYMDALPVLVNPRTGRIHTSYNQTVAATG
                     RLSSSNPNLQNIPIRTMEGKRIRQAFIAAPDCVLISADYSQIELRVLAHLSEDATLLE
                     AFAADEDIHTRTASDIFGVFPEMVNPDMRRQAKVINFGVLYGMSAFGLARELGISQKT
                     AQAYIDGYFARYKKVRDYLDGILEGARRDGFVCTILNRRRYLPELQSRIPSVRQFAER
                     MAINAPIQGSAADLIKVAMVNIDRLLTEKNLSARLIMQVHDELVVEAPVEEKEDVTVL
                     VKKEMEGVMKLKVPLKVEIAAGSNWDEAH"
     gene            complement(3420..3866)
                     /locus_tag="PLR47_05700"
     CDS             complement(3420..3866)
                     /locus_tag="PLR47_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HOG12473.1"
                     /translation="MDAIDKKLLNLLQKEFPLEEEPFRLIGERCGISEDEVLARVRRM
                     KEKGVIRRIGAVFDGKKLGRASTLCAARVPEDKLQSFVHIVSAEKGVTHNYRRGHEYN
                     VWFTVTAANTEELQALLSDVKKKTGVTDMLDMRAVRTFKIDASFDL"
     gene            complement(3999..5099)
                     /gene="ahbD"
                     /locus_tag="PLR47_05705"
     CDS             complement(3999..5099)
                     /gene="ahbD"
                     /locus_tag="PLR47_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme b synthase"
                     /protein_id="HOG12474.1"
                     /translation="MSRPFPKGYASHEGKGNSVRMIAWEVTRSCNLNCVHCRAASHLG
                     PYPGELSTDECLRLVDDIAGVSQPVIILTGGEPLLRPDIFDIAAYGTRKGLRMVMATN
                     GTQVDQAVARKMMESGIQRVSVSIDGQDAESHDAFRNEAGAFAGAMRGIEAMKSAGME
                     FQINTTITTANLRQIKAIHDLVLRVGAAAHHIFLLVPTGRGKDLAQQAITAADYEETL
                     HWFYRESLTCEIQLKATCAPHYFRIMHQNKPKGAKPVKNAGGHFHESTRGCLGGITFC
                     FISHVGQVQPCGYLELDCGNVRKQSFGEIWESSEVFRNLRDYGRYKGKCGRCEFIKVC
                     GGCRARAYEATGDYLAEEPLCLYEPGALKTGF"
     gene            complement(5751..6577)
                     /gene="hemB"
                     /locus_tag="PLR47_05710"
                     /pseudo
     CDS             complement(5751..6577)
                     /gene="hemB"
                     /locus_tag="PLR47_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012702.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
     gene            complement(6581..>6792)
                     /locus_tag="PLR47_05715"
     CDS             complement(6581..>6792)
                     /locus_tag="PLR47_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="12,18-didecarboxysiroheme deacetylase"
                     /protein_id="HOG12475.1"
                     /translation="FSEIWTDEKNEFLMKLKEKKKHVTGRCATCVWLSVCGGNFRARA
                     ESAGDVWGPDPACYLTDEEISSKKE"
BASE COUNT         1399 a         1969 c         1751 g         1673 t
ORIGIN      
        1 tgtagtaatc ggacatggcc tgattttttt cactgatccg cgcccggaaa taataagctt
       61 cgatgttctt cgggtcgagt tcgatggcac ggctgtaagc atcgaaggcc cccttaaaat
      121 tgtcctcggg acctgaaacg gcatgacctt tttcaatcca atccgccgcg gacagttccc
      181 tgatcgactg gtcataggcc gccttctgcg actcccgacc cccgtttttc gccgaagcca
      241 tagccttgcg cagccgctca acctcacgga gcaactcgtc ggatttctgt ttcatcgatt
      301 ccagctcttt tgtcttatct cggtctttgc gcatggcgtc gatggactgg accactttat
      361 ccgaatcggc cacgattttc gatttgagcc agtaaacgtg gccatcccat ttttccgcga
      421 taatttccgt ctggacgatg ccggcagtca acgttgtgat ctggtctttt gtaagctgaa
      481 aattctttac ttccgtttca ctttccaggt aagtccccaa ttcctccagc agcagccgct
      541 tcacttcgcg aagggcgatc acccggctgg aatttttgct gtcctcatcg ccggcctgat
      601 aggtgtattc cttgatgaag gttctggttt cggcaaaagc aacctggccc atcaacggga
      661 caaaaaaaca caaaaaaaga gccattattt ttacagcatg tttcatcgat agtcctgcct
      721 cttaaagatc acaaatccgg attctgtttt taatgcgctt cgtcccagtt agaccccgcc
      781 gcaatctcca cttttaacgg caccttgagt ttcatcacgc cttccatttc ttttttaaca
      841 agcaccgtaa cgtcttcctt ttcttcgacc ggagcctcaa ccaccagctc gtcatggact
      901 tgcataatca aacgggccga cagatttttt tccgtcagga gacgatcaat gttgaccatc
      961 gccactttaa tcaaatcggc ggcggagccc tggatgggcg cattgatcgc catccgttcg
     1021 gcaaactggc gaacggatgg aatccggctt tgcagctcgg gcaggtagcg gcggcgattc
     1081 aggatcgtac agacaaagcc gtcgcgcctt gcgccctcca gaatgccgtc cagatagtcg
     1141 cggacttttt tatagcgggc aaaatagccg tcaatgtagg cctgggcggt cttctgggaa
     1201 atgcccagct ctctggccag accgaacgcg ctcatgccgt aaagaacgcc gaagttgatg
     1261 actttggcct gacgacgcat atccggattg accatttcgg gaaaaacgcc gaagatgtcg
     1321 gacgctgttc gcgtgtgaat atcttcatct gcggcaaagg cttcgagcag cgttgcgtct
     1381 tcggacaaat gcgcaagcac ccgcagctcg atttgtgaat aatcggcgga gatcaaaaca
     1441 caatccggcg ccgcgataaa ggcctgacgg atccttttgc cttccattgt ccggatcgga
     1501 atattctgca ggttggggtt gctgctcgaa agccggccgg tcgccgccac cgtctggtta
     1561 taggaggtgt ggattctgcc ggtccgggga ttaaccaaaa cgggcagcgc atccatgtaa
     1621 gtggacttca gtttggacaa agaacgataa gccagaatct cggcgggcag ttcgtggctg
     1681 cgggccagat ccgtcagcac atcgacatcc gttgaatagc cttccttaat ttttttgccc
     1741 ttcgggagtt tgagtttatc aaaaagaatg gtctgcaatt gctttgggga gttgatgttg
     1801 aatttttccc cggccaggcg gtgaatcttg tcttcggaga tggacagcaa ctgccccaac
     1861 tcatcagaca tctgctgcag caggacggtg tccaccagaa cgcctttttt ctccattccc
     1921 gccagcgtaa acagcagggg catttccaca tcgcgataaa gcgatgcggc gcctgcttct
     1981 tcgagtcggc tttccaggac ctcggccaac gccatgatcg cttccgcgcg ctcgcccgca
     2041 taggcggcca ttttatccac gggcgccagc gacagagcat aggttttccc cttgccgccg
     2101 gcgatgtcat ccggagtgct gatgcgttcg tgaagatatt cccacgccat ttcatcaagc
     2161 gcataggaat gcctggccgg attgagcaga taagcggcca gttgtaaatc aaagccggcc
     2221 cgaatcgtca gatccggcat ggaaacaagt gttgttttca aatcgtaggc gtatttgaca
     2281 atttcagcat tggcaaaaaa agacgcaagc attgaaaagg tttccttggg atccaactgc
     2341 tctttggcag gggtgtgtcc cagcggcaga taaaacgagt cgccacccgc ccggaaagcc
     2401 atgccgatta attcctgcgg agggtttttc tcccgaatca tatcgcaggc gattttggag
     2461 ttgttgtgca acccggctat cagttccgaa agcgacttgc cgtccgccac gaccttgcag
     2521 gctttaaccg gcttttcagc ttttatttta atttgctgca aaagcgaaga aaattcaaat
     2581 tcgccaaaga gcctggccag cgtctccagg tccggcttcc tgcgcgccgc gtcttccaga
     2641 tgaaaatcca tctcaatgtc cgtgcgaatg agcgcgagct gccggctgag acgagcctga
     2701 tccgcataat tgcggaaact ctcgcgcagc ttcacgttgt gcagcttctg tgcgttttca
     2761 atgacggcgt ccaccgaacc gtactcctcg atcagccgcc gggccgtttt cggcccgatg
     2821 cccggcacac cgggaatgtt gtcggatgtg tcgcccgtca gtcccagaat ctccaccacc
     2881 ttttccggcc ccacgccgaa tttttcctcc acggccgccg catcgtaggt tttatctttc
     2941 atcgtatcga ccatggtcac ccggtcatcg ataagctgca tcaaatcctt gtcgccggaa
     3001 atcatgaccg tgcgccaccc tttttgacgg gccgctaccg caagtgtgcc gataaggtca
     3061 tccgcttccg ttccctgctt ttccagaaca cagattgaaa aaccgcgaat gacgtctttg
     3121 atgaacggta cctgcggaat cagatcatcc ggcatgggct tgcgcgtggc tttgtaatct
     3181 tcaaattctc catggcgcgt ggtcggccct ttgagatcaa aggtcacgac aatatagtcc
     3241 ggactaagat ccctgagcag cttcatcagc atggtggtga agccataaat ggcgttggtg
     3301 ggaaagcctt tggagttggt gagcgcagga atcccgtaat aggcgcggta tagataattg
     3361 ctgccgtcta caattgccag gaggggggtt tggttttttt cagccatcgc tcatcgtctc
     3421 tacaggtcaa aggaagcgtc aatcttaaag gttctgaccg cccgcatgtc cagcatatcc
     3481 gtgacaccgg tcttcttctt cacatccgac agaagggctt ggagctcttc ggtgttcgcg
     3541 gcggtcacag tgaaccagac attgtattcg tgcccgcggc ggtaattatg cgtcacgcct
     3601 ttctcggcgc tgacgatgtg aacaaatgat tgaagtttgt cctcgggcac ccgcgccgcg
     3661 cacagagtgc tcgcccgccc gagtttcttc ccgtcgaaca cggcgccgat cctccggatc
     3721 acgccttttt ccttcattct tcggacgcgg gccagcactt catcttcgct gattccacag
     3781 cgctcgccga tgagccggaa cggttcctct tccagcggaa attccttttg cagaaggttg
     3841 agcagttttt tatcgatggc gtccattttt attttcttcc tctttcgggc ttttcaaaat
     3901 gtccagatac aaggcgcgca agtcctgagg aatgagacgt atttttgtat acgtcgcaat
     3961 gacgaaagac gcagcgcaac gccgtagttg agcgtttttc aaaagcctgt cttcagcgct
     4021 cccggttcat agagacacag cggctcctcc gccagataat cgccggtggc ctcataggcc
     4081 cgggcccggc agccgccgca gactttgatg aactcgcagc gcccgcactt gcccttgtaa
     4141 cggccgtaat cccgaagatt gcgaaacact tcggagctct cccagatttc gccaaaactt
     4201 tgtttccgaa cattgccgca gtcgagctcc agataaccac agggctgcac ctgcccgaca
     4261 tgcgaaataa agcaaaatgt aatgcccccc aggcagcctc tggtcgattc atggaaatgt
     4321 ccgcccgcat ttttcacggg ctttgcacct ttgggcttgt tctggtgcat gatgcggaag
     4381 taatgcggcg cgcaggtggc cttgagctgg atttcgcacg tcaggctttc ccggtaaaac
     4441 cagtggagcg tctcttcata atcggcagcg gtgatggcct gctgcgccag atcttttccc
     4501 cgtccggtgg gcaccagcag gaagatgtgg tgcgccgccg cgccgacgcg aagcaccaga
     4561 tcgtgaatgg ctttgatttg acgaagattg gctgttgtaa tggtcgtatt gatttggaat
     4621 tccatcccgg ccgatttcat ggcttcgatg ccgcgcatcg cgcctgcaaa cgctccggct
     4681 tcattgcgga aagcatcatg gctttctgcg tcctggccgt cgatgctgac gctcacccgc
     4741 tgaatgccgc tttccatcat cttgcgcgca acggcctggt ccacctgcgt gccgttcgtc
     4801 gccatgacca tgcgcaaacc cttgcgggtt ccataggcgg caatatcaaa aatgtccgga
     4861 cgcaaaagcg gctcgccgcc ggtcaggatg atgactggct gcgacacccc cgcgatatcg
     4921 tcaacgagac gcagacattc gtcggtggag agttccccgg gataaggtcc cagatgtgac
     4981 gcggcgcggc agtgcacgca gttgagattg caactgcggg tcacttccca cgcaatcatg
     5041 cgcacggaat tccccttccc ctcgtgggac gcgtaccctt tagggaaggg gcgggacaag
     5101 acacccgcat ccacgtccgt tgcacgatca gaatctcccc tcccctcttg ggaggattga
     5161 gagaaagcgc ccgcatctgc gtccgtcgta cgattaaaat ctcccctccc cttgtgggag
     5221 gggcaggggg aggggtgggg ggagtgtgat aaacgctcca taatcgattc caatactcct
     5281 tctatatttc ctaatatttc attattccaa aaacgcaaca ccctgaaacc ttgagaggcc
     5341 agccattgat cgcgctgtcc gtcactttcc tgatgctccg cgtgctggct cccgtccaat
     5401 tcaacaatca atttgtcctc aaatgaaaca aaatcgacaa tgtaaccgcc gatcggttgc
     5461 tgtctgcgaa actttgtgct ttcgagctgc ctggatctga ggtgcttcca taataatcgt
     5521 tccgcatccg ttgaattggc tctgaggttt tttgccaatc gggtcagatt ggttttgtct
     5581 tttttcaaaa gatttcaccc tccccctgcc cctcccttca agggagggga gtctcccgga
     5641 acatcctgcg atctaaccct ctcccatcaa gggaggactg atcacccctc cccttgtgtg
     5701 aaggggcggg gtgaggggga atttccgcac gtcttttcgc gctccacatt tcacttcgcg
     5761 agcgcctttg ccagatcctt cgcccagtag gtgataataa tatcagcgcc ggcgcgtttg
     5821 atggccgtga cgctctccat catggctttt ccctcgtcga gccatcccag ctttgccgcg
     5881 gctttgatta tcgaatattc gccgcttacg ttgtaggcgg caagcggcaa atcgaattcc
     5941 tgtttggccc ggcagatgat gtccaggtac ggcagcgcgg gcttcaccat gataatgtcc
     6001 gcgccttcat tgacgtcgag cgagatttcc cgaatggcct cgtcggaatt ggccggatcc
     6061 atctgatagg ctttgcggtc gccgaactgc ggcgcgcttt cggccgcctc gcgaaacggg
     6121 ccgtaaaacg aggaggcgta cttggcggaa taggccatga tcggaatgtt ttccagcgcg
     6181 ttttcatcga ggatctcgcg aatggccgca atctgcccgt ccatcatggc cgacggcgcc
     6241 accatgtcgg cgccggcttt cacctgcgag agcgccgttt ccgccagtac ttccagcgtg
     6301 gcgtcgtttt gcacctgccc cttttcaata atgccgcagt ggccgtggct cgtatattcg
     6361 cacaggcaga cgtccgtaat caccagcatg tccggcactt cgttcttgac gcgcttcacg
     6421 gcctgctgga cgaggcaatg ggttttttca catccctgcc cggcacggcg aaaagcggat
     6481 aaaccagctc gtccacgctc agacgcgttt cgcgcaccag ccggcgaaag gtgtcgtttt
     6541 ttctcaaccg gcgtccccgg tattcaggaa attgcatacc ctactccttt tttgatgata
     6601 tttcttcgtc ggtgagatag caggccggat ccggccccca gacgtcgccc gccgactcag
     6661 cccgcgcccg aaagttcccg ccgcacacgg aaagccacac gcacgtcgcg cagcggccgg
     6721 tgacgtgttt ctttttctcc ttcagcttca tcagaaattc atttttttcg tccgtccaga
     6781 tttcgctgaa gg
//
LOCUS       DAOSIM010000103         6787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_10182_length_6787_cov_6.975936, whole genome shotgun sequence.
ACCESSION   DAOSIM010000103 DAOSIM010000000
VERSION     DAOSIM010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6787
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10182_length_6787_cov_6.975936"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PLR47_05720"
     CDS             complement(<1..738)
                     /locus_tag="PLR47_05720"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOG12476.1"
                     /translation="MQQPPPNALTRRLSFVQKKIPRWKADGILFTDICNIRYLTGFTG
                     SEGILMAGRDEARLLVDGRYTAQARMEVCGISVFEYADKLQGIEQAAAKMGLSKIGFE
                     PSRMSVGDYNRLSARLKNANLIPLDEELRMLRARKDASEIALMKKAAAIAAKAVSELA
                     QALRPGWTEREAALYLETQARRAGAEQIAFETIIASGENGAFPHAQPSRRKIRHGDFI
                     VVDFGVRCRGYCSDETCTFAIGELTRGQ"
     gene            complement(778..1539)
                     /locus_tag="PLR47_05725"
     CDS             complement(778..1539)
                     /locus_tag="PLR47_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG12477.1"
                     /translation="MKLKAVLMILLVAGQLFFLSCTSTPYHRDQADIHLQKGVALLQA
                     GKHIGALGELLEAEKNAPDDPEIRYYLGIAYLGRGLKDQSMEQFQKAVSLKEDYSEAH
                     NYIGVMQMDAGEWQPAIDSFDRALKNYLYPTPAFALYNSGVAYYNLQNYDMALSRFQR
                     ALSQDGVAAWQPQVELNIGRVYIQKANYGKAKEHLEKAVALNPSLFDAHFYLGETYLK
                     LRDKAKAKKSFEQVIKLAPKSALGQKSREYLKTLP"
     gene            complement(1651..3348)
                     /locus_tag="PLR47_05730"
     CDS             complement(1651..3348)
                     /locus_tag="PLR47_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HOG12478.1"
                     /translation="MRKYLSRIIPVFIVLWVFFYTPAGVVTGSGAPDNAGYLEKILLE
                     DQPGREKVILEVSQLPTNIPASLQGDNSLLVRLENLYVPNHLRETLKGNLQNITDVRA
                     EQKTERGKPWVYLTIRFRENTPYSIVPEGNRVIVDFNISGVASKLGESLLQNAAGEKS
                     QARPGNVKRYTDRIVSLDFQDADIKSVLRLMAEYGNISIIAGDDVKGNVTVTLKSVPW
                     EQALDSILNIKGLTKKQTEEVIIVMTLAKKKQDEADRQAAFLAQQKAEEEQKAREQKS
                     DIEKGKLRQIMIEAKIVEASDDFIRNLGIQWGGGNTQNIAGAYGLGITGATNPLTTTD
                     KFAYAFPAEINPAMAMAAVNMPSSALAGPTLGLVFGGARGFIEAQLQALEKTTQGKII
                     SSPRVVTMDDVKAIIKQGEEIPYVTPATTTSPATIAFKDALLKLEVKPKITDDNRISM
                     DIKATNDRADYTKALVAGQQMPIIKNEVESKVVVKDGDTVVIGGVAKVDETKITAGVP
                     WFYKIPVLGWLFKTENVDIKKRQLLVFITPKILTESGYVDDECDTTKVDNLDRCRRK"
     gene            complement(3361..3918)
                     /locus_tag="PLR47_05735"
     CDS             complement(3361..3918)
                     /locus_tag="PLR47_05735"
                     /inference="COORDINATES: protein motif:HMM:NF016254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HOG12479.1"
                     /translation="MKRKNKKQWILLFGLWLFLATAFCACGEQEKKPAASPPPAKPQA
                     VQTAPAAQPKAVQTAVAAVPAVQKPESVYSYRPSGKPDPFRPLVDTKIAAASKVKAAK
                     QQSIFPLQKAGAENFTVVGIMGDENRRMAVVEDAAKKFYPLFIGTSMGLNNGKVTDIQ
                     ADRVIVEEREGKKARRVVLTLRKNQ"
     gene            complement(3902..4657)
                     /gene="pilO"
                     /locus_tag="PLR47_05740"
     CDS             complement(3902..4657)
                     /gene="pilO"
                     /locus_tag="PLR47_05740"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOG12480.1"
                     /translation="MAMDLKLDELKKLSPQVKALIVVVVVFLIGYLFYFYFLSDALAK
                     RSEMTKEYEELQAQIQQKENIARQINKVKAQVASLKENYTAALLKLPARKEIPALFYS
                     VATAGREAGVEFVLFEPQASVPQTLEKAEKLSGKLKPSDKRQEEQEKKAEAEKAAGKP
                     ADAKKPPAKAPAPEPFYEEIPVKVTVAGGFQNLVFFFDKLAKLPRIVNISEVSIGDRK
                     ESKAGKKYSITASCIVKTYMFVDKKEQANEKKK"
     gene            complement(4661..5209)
                     /locus_tag="PLR47_05745"
     CDS             complement(4661..5209)
                     /locus_tag="PLR47_05745"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOG12481.1"
                     /translation="MIKINLIPYHEKEKKEDLARQIAVMAGAFLLFILCLVGVYAYMT
                     VSIGGLETRLTESREKLKALDAKVGDLGKFKSQIAEFELKLGVIKTLEENRVYPVKLF
                     DDLAMQVPPKSVWLTKIGQSQESLTIEGIGRDNIAVAGFMKTIENFPAMQSVDLVSSK
                     KVEIAQTTLQQFIFSCKLKKGF"
     gene            complement(5206..6279)
                     /gene="pilM"
                     /locus_tag="PLR47_05750"
     CDS             complement(5206..6279)
                     /gene="pilM"
                     /locus_tag="PLR47_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOG12482.1"
                     /translation="MDLKNLFSGTKSLVGLDIGSTSTKLAEIVATSQGSTLNRFFQVP
                     MPPGVIVEGILQDPGALSGVIKELFRHSGCKGKGIVTSLSGNSVIIKKVTLAQMEETE
                     LRELIHDEAGKYLPFDNMDDVNYDFQILGDNEFNPNQMDVIIVAAKKTDVNGYLDAFA
                     AAGLTVMIMDVDSFALETMYEANYDFEENEIIVIVNIGASLTNINVIKGGMSVFTRDF
                     TMAGNAITEALQERFQVTAEEAERMKVEGTAGDKEIPDLENVILDAAEPICSEIERSV
                     DYFRSTFGGDEIKQVLLSGGSSRIAGLSDLLSQRLNIGTQLVNPLAKIGYNKKNIDEG
                     KLDSIRTIGAVAIGLGLRKIGDK"
     gene            complement(6313..6579)
                     /locus_tag="PLR47_05755"
     CDS             complement(6313..6579)
                     /locus_tag="PLR47_05755"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOG12483.1"
                     /translation="MKIGRYLAAFVFLMMLLITFGNRGVVDNYLIAKRLSQVKAENNA
                     LIAQNKGLAGKIHLLRTDPAYIEAIARNELGMVKPGDVVYRLAR"
BASE COUNT         1383 a         1908 c         1739 g         1757 t
ORIGIN      
        1 ttgaccccgt gtcaattccc caattgcaaa cgtgcaggtt tcatcggaac agtatccccg
       61 gcagcggacg ccgaaatcca ccacgatgaa atccccgtgc cgaatcttcc ggcgggaagg
      121 ctgcgcgtgc ggaaacgcac cgttttctcc ggaggcaata atggtttcaa aggcgatctg
      181 ctccgcgccc gcgcgccgcg cctgcgtttc caggtacagg gccgcttccc tctccgtcca
      241 gccgggacgc agagcctgcg caagctcact caccgccttt gccgcaatcg ccgccgcttt
      301 tttcatcagg gcgatttccg acgcgtcctt gcgggcgcga agcattctca gctcttcgtc
      361 gagcggaatc agatttgcat ttttcagccg ggccgaaagt cggttatagt cgcccacgct
      421 catgcgcgac ggctcgaaac cgatttttga aaggcccatt tttgcagcgg cctgctcgat
      481 gccctgcagt ttgtcggcat actcgaaaac ggaaatcccg cagacctcca ttcgggcctg
      541 cgccgtgtag cgtccgtcca ccagcagccg ggcttcgtcc cggcccgcca tcaggatgcc
      601 ctcactgccg gtaaatccag tgaggtagcg aatgttgcaa atgtcggtaa aaagaattcc
      661 gtccgctttc cagcggggaa tttttttctg cacaaaagac agtcttctgg tcaatgcgtt
      721 gggggggggc tgctgcatga agggcggttc ctgtacatcg gccggttttt ccggcggtta
      781 cggcaacgtt ttcaaatact cccgggattt ttgtcccaaa gccgacttgg gcgcaagctt
      841 gatcacctgt tcaaaggatt ttttggcctt tgctttatcg cgaagcttta aataagtttc
      901 tcccagataa aaatgcgcgt caaaaagaga agggttgagc gccaccgctt tttccagatg
      961 ttctttcgcc ttgccataat ttgctttttg aatatagacc cgaccgatat tcagctcgac
     1021 ctgcggctgc caggcggcca ccccgtcctg gctcagagcc ctctggaacc gcgacagggc
     1081 catgtcataa ttttgaaggt tgtagtaggc cacaccggag ttgtaaagcg caaacgcggg
     1141 cgtcggatag agataattct tcagcgcccg gtcaaaggaa tcgatggccg gctgccactc
     1201 ccctgcgtcc atctgcatga cgccgatgta attatgagcc tcggaataat cttccttgag
     1261 ggacacggcc ttctgaaact gctccatcga ctgatctttc agtccgcggc cgagataggc
     1321 aatgccgaga taatagcgga tttccggatc atccggcgcg tttttttccg cttccagaag
     1381 ttcgccaagc gcgccgatat gctttccggc ctgaagaaga gccacgcctt tttgcagatg
     1441 aatgtcagcc tggtcccggt gataaggcgt gctggtgcag gacagaaaaa acaactggcc
     1501 cgcgacgagc agtatcataa gaacagcttt cagtttcatg atcccatcag cttccttgat
     1561 ttcttcggat gaaaattcat ctgaacggat tcataaaaat ccctccgcgg aacgtcacgc
     1621 ggagggaaaa atgttggagc aaacaaaaat ttatttccgg cggcagcgat ccagattgtc
     1681 cactttcgtc gtgtcgcatt cgtcgtcaac gtatccggat tccgtcagaa ttttcggcgt
     1741 aatgaagacc aggagctgcc gcttcttgat gtcgacattt tccgttttaa acagccaccc
     1801 cagaacggga atcttgtaaa accagggaac gccggccgtg attttggtct catccacttt
     1861 ggcaacgccg ccgatcacga ccgtatcgcc gtcctttacg accactttgg attccacttc
     1921 gtttttgatg atgggcatct gctggcccgc aaccagcgcc ttggtataat ccgcccggtc
     1981 gttcgtcgcc ttgatgtcca tggaaatccg gttgtcgtcg gtgattttcg gcttgacttc
     2041 cagcttcaga agagcgtctt taaaggcgat ggtggcggga gacgtcgtcg tggccggcgt
     2101 cacgtaggga atctcctcac cctgcttgat gatggctttg acgtcatcca tcgtcacgac
     2161 gcggggcgaa gaaatgatct tgccctgagt ggtcttttcc agcgcctgca attgcgcctc
     2221 gatgaatccg cgggcgccgc cgaacaccag gcccaaagtt ggcccggcca aggcagatga
     2281 aggcatattc actgccgcca tggccattgc cggattaatt tcagccggaa aagcatatgc
     2341 aaatttatca gttgtggtca gcgggttggt tgcgcctgta atccccagcc cataagcgcc
     2401 ggcgatgttc tgtgtgttgc cgccgcccca ctgaatgccc aggttccgga taaaatcatc
     2461 cgaagcttcg acgattttgg cctcgatcat gatctggcgc agcttccctt tttcgatatc
     2521 ggacttctgc tctctcgcct tttgctcctc ttccgccttc tgctgggcca aaaaggccgc
     2581 ctgccggtcc gcctcatcct gtttcttttt ggcaagcgtc atcacgatga tgacttcttc
     2641 cgtctgtttt ttcgtaagcc ccttgatatt cagaatggaa tccagcgcct gctcccacgg
     2701 aacgcttttc agcgtcaccg tgacattgcc tttgacgtcg tcgccggcaa tgatgctgat
     2761 attgccgtat tccgccatca accgcagcac gcttttgatg tccgcatcct gaaagtccag
     2821 agagacgatc cggtccgtat atcgtttaac gtttcccgga cgcgcctgcg atttttcacc
     2881 ggcagcattt tgaaggaggc tttcgcctaa cttcgaggcc acgccggaaa tattgaaatc
     2941 aacaatgact ctatttcctt cgggcacaat ggagtaaggc gtgttttccc tgaagcgaat
     3001 ggttagataa acccagggct tgccccgctc cgttttctgt tcggcccgca catcggttat
     3061 gttttgcaga ttgcctttca gcgtttcccg cagatggttc gggacataca aattctccag
     3121 ccggaccagc aggctgttgt ccccctggag agacgcggga atatttgtcg gcagttgaga
     3181 aacctccaga atgacttttt cccgacccgg ctggtcttcc agcaggatct tttccagata
     3241 accggcattg tccggcgcgc ccgaacccgt caccacgccg gccggtgtat agaagaaaac
     3301 ccagaggacg atgaaaaccg gaatgattct ggataaatat tttctcatga tcttacctca
     3361 ttattgattt ttacgcagtg tcaaaaccac tcttcttgct ttttttcctt cccgttcctc
     3421 cacgatcacc cggtccgcct ggatgtcggt tacttttccg ttgttaagcc ccatgctggt
     3481 tccgataaaa agcggataaa actttttggc cgcgtcttcg acaacggcca tgcggcggtt
     3541 ttcatcgccc atgatgccga caaccgtgaa gttttccgcc ccggctttct ggagcgggaa
     3601 gatggattgc tgtttggccg ccttgacttt ggacgccgcc gcgattttcg tgtccacgag
     3661 cggcctgaag ggatccggtt tgccggacgg ccggtagctg taaacgctct ccggcttctg
     3721 gacggccggc actgctgcga ccgcggtctg aacggctttc ggttgagcgg ccggcgcggt
     3781 ttgaaccgcc tgcggttttg ccggcggcgg ggacgcggcc ggtttctttt cctgttcccc
     3841 gcaggcgcag aatgccgttg ccaggaaaag ccataaaccg aacagaagga tccattgttt
     3901 cttatttttt cttttcattg gcctgctctt ttttgtccac aaacatgtag gttttcacga
     3961 tgcaggatgc cgtgatcgaa tatttcttcc ccgccttgct ttcttttcga tccccgatgg
     4021 aaacttcgga aatattgacg atccggggca gtttggccag cttgtcaaaa aagaaaacaa
     4081 ggttctggaa cccgcccgcc acggtcactt tcacggggat ttcctcgtag aaaggctccg
     4141 gcgccggcgc tttggccggc ggttttttcg cgtctgcggg cttgccggcc gctttctcgg
     4201 cttccgcctt cttttcctgc tcctcctgcc gcttgtcgga gggtttcagc ttgcccgaga
     4261 gtttttcggc cttttccagg gtctgcggca cggaggcctg gggttcaaac aggacgaact
     4321 ccacgcccgc ttcccggccc gccgtggcga cggaataaaa gagggccgga atttccttcc
     4381 gggcgggaag cttcagcagg gcggccgtgt aattttcttt cagggacgcg acctgcgcct
     4441 tgaccttgtt gatctggcgc gcgatgtttt ctttctgctg aatctgcgcc tgaagctcct
     4501 cgtactcctt cgtcatctcc gatcttttcg caagcgcgtc ggacagaaaa tagaagtaga
     4561 acaggtagcc tatcagaaaa acgaccacga cgacgatcag ggccttgacc tgcggagaca
     4621 attttttcag ttcgtctaat tttaaatcca tcgccatggc ttaaaacccc tttttcaact
     4681 tgcaggaaaa aatgaactgc tgcagggtcg tttgggcaat ctccactttt ttggacgaca
     4741 ccaggtccac cgattgcatc gcgggaaaat tttcgatcgt tttcatgaaa ccggcaaccg
     4801 cgatgttgtc gcggccgatg ccttcgatgg tcaggctttc ctggctttgt ccgattttcg
     4861 taagccagac gcttttgggc ggaacctgca tggccaggtc gtcaaacagc ttgaccgggt
     4921 aaacgcggtt ttcttccagc gttttaatga cgccgagctt cagctcgaat tcggcaatct
     4981 ggcttttgaa tttgcccaga tcacccacct tggcatccag cgccttgagc ttctcccggg
     5041 attccgtgag cctggtttcc aggccgccga tcgaaacggt catgtaagcg tacacgccga
     5101 caagacaaag aatgaaaagg agaaaggccc cggccatcac ggcaatctgc cgggccagat
     5161 cctctttctt ttctttttca tggtatggga taagattgat tttaatcatt tatcacctat
     5221 tttccttaaa cccaacccga tggccacagc gccgatggtt ctgatgctgt ccagttttcc
     5281 ctcatcaata ttttttttgt tgtagccgat cttggcaagc ggattgacaa gctgcgtgcc
     5341 gatgttcagt ctctgggaca aaaggtcgct caatccggca atcctcgaag atccgccgga
     5401 cagcaggacc tgcttgattt catctcctcc gaacgtggac cggaaataat cgacggatcg
     5461 ttcgatttcc gaacagatgg gctcggctgc atccagaatg acgttttcca gatccgggat
     5521 ttccttgtcg ccggccgtcc cttccacctt catccgctcc gcctcttccg ccgtcacctg
     5581 gaatctttcc tgcagcgcct ccgtgatcgc gtttccggcc atcgtgaagt ctctcgtgaa
     5641 gacggacatg ccgcccttga tcacgttgat attggtcagg ctcgctccga tgttgacgat
     5701 gacgatgatt tcgttttctt caaagtcgta gttggcctcg tacatggttt ccagggcgaa
     5761 ggaatccacg tccatgatca tgaccgtgag gcctgccgcg gcaaacgcgt ccaggtagcc
     5821 gttgacgtcc gtttttttgg cggcgacgat gatgacgtcc atctggttgg ggttgaattc
     5881 attgtccccc aggatctgga aatcgtaatt cacgtcgtcc atgttgtcga aagggagata
     5941 tttgccggct tcgtcgtgga tcagttcgcg cagctccgtt tcttccatct gggccagcgt
     6001 cacttttttg atgatgacgg aatttccgga gagggaggtg acaatgccct tccccttgca
     6061 gccggaatgg cggaaaagct ccttgatcac gccggacagg gcgccggggt cctgcagaat
     6121 gccttccacg atgacgccgg gcggcatggg aacttgaaaa aaccggttaa gcgtcgaacc
     6181 ctgagaggtg gcgacgatct cggccagttt cgtggaggtc gatccgatat ccagaccgac
     6241 gaggcttttg gttccagaaa ataagttttt taaatccatg cctgcaccgt gtatgggttc
     6301 acatgcttcc ggttaccggg ccagccggta taccacgtct ccgggtttga ccatgcccag
     6361 ttcgttgcgc gcgatcgctt caatataggc cgggtcggtt ctgagcaaat ggatctttcc
     6421 cgccagtcct ttgttttgcg cgatcagtgc attgttttcc gctttcactt gtgacagtct
     6481 tttggcgata agataattat cgacaactcc ccggttgccg aaggtgatca acagcatcat
     6541 caaaaaaaca aaggcggcca gataccttcc gattttcatg gatcaacgct ccagacaccc
     6601 tatccgtatt aaaataaatt cacttgtggt ttaaaaaact acaattggga gcaaaagtca
     6661 agcaaagaaa tttcatttgc gttaaattta ttgatcaatt gatccttccc ccctgaatca
     6721 ggcaaaaggt cccgagggca gattctgctg cggctctccc agcagaaatt ttccggcttc
     6781 gatccag
//
LOCUS       DAOSIM010000104         6718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_10317_length_6718_cov_6.067087, whole genome shotgun sequence.
ACCESSION   DAOSIM010000104 DAOSIM010000000
VERSION     DAOSIM010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10317_length_6718_cov_6.067087"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PLR47_05760"
     CDS             complement(<1..435)
                     /locus_tag="PLR47_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOG12484.1"
                     /translation="MENELSLKVEKTGHVAWLILNRPKQRNTMTIDFFQEMQRLFRAF
                     DEDADVRVVVIRAEGKSFTAGLDLVAAQSLLGDGSAVYREELRRKIMDLQASITAIEN
                     CRKPVIAAIHGHCIGGGVDLTSACDIRLASKDAVFSIRETRIG"
     gene            831..1175
                     /locus_tag="PLR47_05765"
     CDS             831..1175
                     /locus_tag="PLR47_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12485.1"
                     /translation="MEDYVHHTVGEEVNFIAGNYQIVEEGCLTHDGREFLYLMGIATV
                     DNACCGQTGCRFLLVPGYIHSWKTKRNPQGKPVSEVEPITDEQKQAAIRAFLEACYPY
                     AQINFSGKSHFS"
     gene            1339..2049
                     /locus_tag="PLR47_05770"
     CDS             1339..2049
                     /locus_tag="PLR47_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG12486.1"
                     /translation="MKSPLEKARKNPESMKARILAVARRIFGEYGFHGTTTRMIAKEV
                     GIDISTLHYHWGDKNDLYEAVVMDVNNDLGRELLKVEKIVHGSSLEERLNVSIDKMMD
                     YLFDHPEISNLVLLRYFSKTRNEIDVDFKVPEFSGDIARAMGLAKDAKNPSPRSMMKV
                     LSIMNSMHSFVSGENFFRPMLKLDRKNYIRAVKETLKFLLIPAFVLQEGKTKKGGDSP
                     EAPLAKEKKKARPAKTAE"
     gene            complement(2129..3007)
                     /locus_tag="PLR47_05775"
     CDS             complement(2129..3007)
                     /locus_tag="PLR47_05775"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG12487.1"
                     /translation="MRYEGNIFRPFSEANSYLLQCTIGCSHNQCTFCGMYKDAKYRVR
                     RTAEILDDIRMAKDYYGDVEKVFLCDGDAIAIETETLLEIIHALYAAFPSLRHVGSYV
                     GPQSTLAKSPTELRALRSAGLTKAYIGVETGDDELLKKVRKGVGSAEMLEAGRRLVDA
                     GINLSAMVLLGLAGPGEPSRRHALATAKICDEMKPQYLAALTVTPVLGTVLYRQVQKG
                     EFQLLDPFETLEEMKMMLENITLNPLKFVGTHASNYLPVTGTLQKDKPKMIALVDQVL
                     TGRDERLLRPDHLRGL"
     gene            complement(3028..4008)
                     /locus_tag="PLR47_05780"
     CDS             complement(3028..4008)
                     /locus_tag="PLR47_05780"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOG12488.1"
                     /translation="MIIQCKQCRTKFRFDDAKIAQDGIWVRCNRCQHVFFQEPPRRPL
                     SGPAAEGDRPLPSGSAYEKTQSRLSLEPASPPPGKIPRDEDLVGFLRDVMETEKAEQG
                     EAKQDFPDTDRGKDAGAERSGEWSGEEESPPLPARKKNRPWMFALWTLLVVVVIPAVF
                     YFVVFPPLGERLLKAGGQGWESVLSALGFSQPAERLPVTAQVKLQDVRQRVLENYILG
                     PVRIVEGKAVNEAEFPISRVQVRAEILDAYAVVLGERVSYAGNILNDDELITMTEEEM
                     RIRLSLPEGRDQSNDRVEPGGKIPFMVVFTGNPPGMIKTVVRIAGAERLL"
     gene            complement(4005..4559)
                     /gene="hpt"
                     /locus_tag="PLR47_05785"
     CDS             complement(4005..4559)
                     /gene="hpt"
                     /locus_tag="PLR47_05785"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOG12489.1"
                     /translation="MADVLKKVLLEPSEISGRVSEMAGAISRDYAGREILVIGVLKGA
                     FVFMADLIRALSIPCQTDFIRAASYGAGTESSGKIAITKDIEMDVAGRDVLVVEDILD
                     SGLTLRYIVDALKLRNPRSVKVCAFLDKRKRRKVHFEADYVGYTMEDGFVVGYGLDFN
                     EQYRFLPGVYVLDPAPEGEKEANE"
     gene            complement(4632..5450)
                     /locus_tag="PLR47_05790"
     CDS             complement(4632..5450)
                     /locus_tag="PLR47_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12490.1"
                     /translation="MPTEFPIMVLCQPDPGKSCGACCGLYNYVDSSRESLVKRLSART
                     RRYHEMVKGPEDLPRYAKATFAAEDFRKRYEVIYCCEYLGFLDAEEKKVGCLVHPLQN
                     GGVDMRTVSFYGRDVCAGHLCPSHHFIPRSQQLILLKIIDDWYLYGLCLTDIDLVVSW
                     FRLVADRVGEELKPEAFERQDLKEAALQYFRWKMTWPFRCEEANRLGKYYFDGSQYMI
                     SHIDYEKFGRDISPLNAILLSLSSEFHAAEELDQAEKMIWSNVEALALLYRGRL"
     gene            complement(5465..6322)
                     /gene="purU"
                     /locus_tag="PLR47_05795"
     CDS             complement(5465..6322)
                     /gene="purU"
                     /locus_tag="PLR47_05795"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOG12491.1"
                     /translation="MDESNAILLLSCPDRKGLVARLSSFIFQNNGNIVAADQYTSKRG
                     EYFFMRMEWELNGFALSREEIPVVFAPLARQYDMKWTLHFTDYRSRMVIFVSKHLHCL
                     HDLILRRHMGELSAAIEAVISNHNDAKDLVEQFGIKFFHIPITAENKRQQEERQLALL
                     REMKIDLVVLARYMQILTGGFIGQYVNRMINIHHSFLPAFSGSNPYQQAYDRGVKIIG
                     ATAHYATEQLDDGPIISQDVVKISHRDTITDIQTKSKDLERIVLARALRLHLENKIIV
                     YGAKTVVFE"
     gene            6445..>6718
                     /locus_tag="PLR47_05800"
     CDS             6445..>6718
                     /locus_tag="PLR47_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12492.1"
                     /translation="MKRIETIHLPNGLMLTINDLSRRIAADTVKVELSFQMKVKVEPS
                     FFASPDDHRQLTGIFGDELTYEHKLERTFVAEAEKSATRADLLETFK"
BASE COUNT         1590 a         1896 c         1724 g         1508 t
ORIGIN      
        1 gccgattcgg gtttcccgaa tggaaaagac ggcgtctttc gatgccagcc ggatgtcgca
       61 ggcgctggtc agatcgacgc ctccgccgat gcagtgtccg tgaatcgcgg caatcacggg
      121 cttcctgcag ttttcgatgg cggtgatgct cgcctgaagg tccatgattt ttctgcggag
      181 ctcttcccgg tacacggccg atccgtctcc gagcaaagac tgggccgcaa ccaggtccaa
      241 tccggcggta aagcttttcc cctccgcccg gatgacgacg acccgcacgt cggcgtcttc
      301 atcaaatgcc ctgaaaagcc gctgcatctc ctgaaaaaaa tcaatggtca tcgtgttgcg
      361 ctgcttcggc ctgtttaaaa tcagccaggc gacatggccg gttttttcga ctttgagcga
      421 caattcgttt tccatgccga gctctcctta tgattcaata aaggaagcga tcagtgtctt
      481 ttaggaatcg cgccgatctt cggctttccc gcaaacgcat ccaatcaaac attccatctt
      541 ttaattccat caattgatgg gattagtatt tgaaatcttc cgccttgtca agcgttttat
      601 tcaataattt gaaaaaattt acggcacggt tccagaatcc tgtatttatt gtcgattgcc
      661 ggcgggtgcc ggcgtgcgcc tgccggggat gcggcgggat gcttctcttt tgaggcaaaa
      721 caatccggat aaaccatctt gactttttat gtgttttaca atatcggcac cctcggttga
      781 gcgtgaaggc aaacgggaaa aatcgttaac gaatttggag gaggcgcgca atggaagact
      841 acgtccacca cacggtcggc gaagaagtga acttcattgc gggtaattat cagatcgtcg
      901 aagaaggatg tctgacgcac gacggaagag agtttctcta tctgatggga attgcgaccg
      961 tggacaatgc gtgctgcgga caaaccggct gccggttcct tctggtcccg ggctacattc
     1021 attcatggaa gacaaaaagg aatccgcagg gaaaaccggt cagcgaagtg gagccgatca
     1081 ccgacgaaca gaaacaagcg gccatccggg ctttcctgga agcttgctac ccttatgcgc
     1141 agatcaattt ttccggaaaa tcacatttct cgtaaaaaca gacggcggtt gttcaaagga
     1201 tggcacaggc ggcaaagtcg ctcgcaaaca tttccagcct tttttcggaa taatccccca
     1261 atgtctttgg caaatcagaa aaaccttgac gatggaatgg cattatcgta gtattcaatc
     1321 aaatgatgga gtcacaaaat gaaatcaccc ctcgaaaaag cccgcaaaaa ccctgagtcc
     1381 atgaaggcaa ggattcttgc cgtcgcccgc cgtattttcg gcgaatacgg ctttcacggg
     1441 accaccaccc gcatgatcgc caaagaagtg ggaatcgaca tctccaccct tcactaccac
     1501 tggggcgata aaaacgatct gtatgaagcc gttgtgatgg atgtcaacaa cgatctggga
     1561 agagagcttc tcaaagtgga aaaaatcgtc cacggcagct cgctggaaga gcgcctgaac
     1621 gtctccatcg acaagatgat ggattatttg tttgaccatc cggagatatc gaaccttgtt
     1681 ctgcttcgct atttcagcaa aacccgcaac gagattgatg tggatttcaa agtgccggaa
     1741 ttcagcggcg acatcgcccg cgccatgggg ctcgcgaaag acgcaaaaaa tccatcgcca
     1801 aggtccatga tgaaagtcct gagcatcatg aacagcatgc acagttttgt ctccggagag
     1861 aattttttcc gtccgatgtt gaaactggac agaaaaaatt acatccgtgc agtcaaggaa
     1921 accctgaagt ttctgctcat ccccgccttt gtcctccagg aagggaaaac caaaaaggga
     1981 ggggacagtc ccgaagcccc tcttgccaaa gaaaagaaga aagcacggcc ggcaaaaacg
     2041 gcggagtaaa caggatcatc cctgtttatg ccatttccgc ctgccgaacc ttaaaccgtt
     2101 cttccggttt ttttccgaat ccccggggtt acagaccccg cagatgatcc ggccttagca
     2161 gccgttcgtc ccggccggtc agaacctgat ccaccagggc gatcattttg ggcttgtctt
     2221 tctgaagcgt gccggtgacc ggcagataat tggacgcgtg cgtgccgaca aatttcagcg
     2281 ggttcagggt gatgttttca agcatcattt tcatctcctc cagggtttca aaaggatcca
     2341 ggagctgaaa ttcgcctttc tgcacctgtc ggtagagcac ggtccccaaa acgggcgtga
     2401 ccgtcagggc cgcaagatac tggggcttca tttcgtcgca gatttttgcg gtggcaaggg
     2461 cgtggcgccg tgaaggctct cccgggccgg caagccccag cagaaccatc gcggacaaat
     2521 tgatccccgc atcaaccagc ctccgcccgg cttccagcat ctcggcgctc ccgactccct
     2581 tgcgaacctt tttcagcagt tcgtcgtcgc cggtttcaac gccgatatac gctttggtca
     2641 ggccggcgga ccgaagcgcc cgcaattcag tcggcgattt ggcaagcgtg ctttgcggcc
     2701 ccacatagct gccgacatgc cgcagcgagg ggaatgctgc ataaagcgca tggatgattt
     2761 caagcagggt ttcggtctcg atggcaatcg catcgccgtc gcacaagaac accttttcca
     2821 cgtccccgta ataatccttt gccatgcgga tgtcgtccag aatctcggcg gtccgccgta
     2881 cgcgatactt tgcgtcttta tacatgccgc agaaggtgca ttgattgtgg gagcagccaa
     2941 tggtgcattg aagcaagtaa ctattggcct cactgaaggg ccggaatata tttccttcgt
     3001 atctcacaaa atccttcctt gtcgcctcta taacaggcgc tccgcgccgg caatcctgac
     3061 aacggttttg atcatgcccg gcgggttccc ggtgaaaacc accatgaaag gtattttccc
     3121 tccgggttcc acccggtcgt tcgattgatc ccggccttcc ggaagcgaca gccggattct
     3181 catttcttct tcggtcatgg taatgagctc gtcgtcattg aggatatttc ccgcgtagct
     3241 cacccgttcg ccgaggacca ccgcgtaggc atccaggatt tccgccctga cctgcacgcg
     3301 ggagatggga aattcggcct cattgaccgc tttgccttcc acaatgcgga caggccccag
     3361 aatgtaattc tccaggaccc gctggcggac gtcctgaagc ttgacctgcg cggtgaccgg
     3421 cagacgctcc gccggctgag agaagccgag cgcggaaagc acgctctccc acccctgccc
     3481 tccggctttc agaagacgtt cccccagcgg cggaaaaacg acaaaataaa atacggccgg
     3541 gatgacgaca accactaaaa gcgtccacag cgcaaacatc catggtctgt tctttttgcg
     3601 agcgggaagg ggcggcgatt cttcttcccc tgaccattct ccggaccttt ccgcacccgc
     3661 atcttttccg cggtccgtgt cgggaaaatc ctgctttgct tccccctgct ccgccttctc
     3721 cgtttccatc acatcccgaa gaaagccgac caaatcttca tcccggggaa ttttgcccgg
     3781 aggcggactt gccggctcga gggacagacg gctctgtgtt ttttcatagg cggagccgga
     3841 cggaagagga cgatcgcctt ccgccgcggg accggacagg ggacgcctgg ggggctcctg
     3901 aaaaaaaacg tgctgacagc ggttgcagcg aacccagatc ccgtcttgtg cgatttttgc
     3961 gtcgtcgaag cggaatttgg tgcggcattg cttgcactgg ataatcattc gtttgcctct
     4021 ttttcgcctt ccggcgcggg atccagcaca tacacgcccg gcagaaaccg gtactgttcg
     4081 ttgaaatcca gtccgtagcc gaccacgaaa ccgtcttcca tcgtatagcc gacgtaatcc
     4141 gcttcaaaat gaacttttcg tctttttcgc ttgtccagga acgcgcagac cttgacggac
     4201 cggggatttc tgagcttcaa cgcatccacg atgtaacgca gcgtcaggcc gctgtccaga
     4261 atgtcctcga cgaccagaac atccctgccc gcaacatcca tctcgatgtc cttggtgatc
     4321 gcgattttgc cggagctctc cgtcccggcg ccgtagctgg ccgcccgaat aaaatccgtt
     4381 tgacagggaa tggagagagc gcggatcaaa tccgccataa aaacgaaggc gcctttgaga
     4441 accccgatca ccaggatttc gcggcccgcg taatcccggg aaatggcgcc ggccatctcc
     4501 gaaacgcggc cggatatctc cgacggctcc agcagtactt tttttaaaac atcggccatg
     4561 tccccgcgcc tccacgatga tgaaactgcc cgttgatatt actcaagatt cctttgggct
     4621 gtcaatcaaa atcaaagccg gccgcggtaa agcaacgcca atgcctctac gttggaccaa
     4681 atcatttttt ccgcttggtc cagctcctcc gccgcgtgaa attccgagga aaggctcagc
     4741 aggatggcat tgagcggcga aatatcccgg ccgaattttt catagtcgat gtggctgatc
     4801 atgtactgcg atccgtcaaa ataatatttc cccagacggt tggcctcttc acagcggaaa
     4861 ggccacgtca tcttccagcg gaagtattgc agcgcggcct ctttcaggtc ctgcctttca
     4921 aacgcctccg gtttgagctc ctctccgacc cggtcggcca ccaggcgaaa ccaggaaacc
     4981 acaaggtcga tatccgtcag gcagagaccg tacagatacc agtcgtcgat gatcttcagc
     5041 aaaatcagct gctgggacct gggaatgaaa tggtggctgg ggcacagatg accggcacag
     5101 acatcccggc cgtaaaagga aacggtgcgc atgtccacgc cgccgttctg caggggatgc
     5161 acgaggcatc cgactttttt ttcttcggcg tccagaaaac cgaggtactc gcagcagtaa
     5221 atgacttcgt agcgctttcg gaagtcttca gcggcaaacg tcgctttggc gtaacggggc
     5281 agatcctccg gacccttcac catctcgtga tagcgccggg tgcgggcgga aagcctcttg
     5341 accagcgact cgcgtgagct gtccacataa ttgtaaaggc cgcagcaggc gccgcaggac
     5401 ttgccggggt ccggttgaca caaaaccata atgggaaatt ccgtgggcat gaaaagcact
     5461 tctcttattc aaacacgacc gtcttggccc cgtacacgat aatcttgttt tccagatgca
     5521 ggcgcagcgc gcgcgccagc acaatgcgct ccagatcctt gcttttggtt tgaatatccg
     5581 taatcgtgtc gcggtggctg attttaacga cgtcctgaga aataatggga ccgtcatcca
     5641 actgttcggt cgcgtaatgg gccgtcgccc cgataatttt cacgccccga tcgtacgcct
     5701 gctggtaggg attgcttccc gaaaacgcag gcagaaagga atggtgaata ttgatcatcc
     5761 ggttgacata ctgtccgatg aatccacccg tgagaatctg catgtagcgc gccagaacca
     5821 ccagatcgat tttcatttcc cgcaacaggg ccaattgcct ttcctcctgc tggcgtttat
     5881 tctccgccgt gatgggaata tggaaaaatt tgattccaaa ctgctccacc agatccttcg
     5941 cgtcgttatg attgctgatc accgcttcaa tggcggcgct cagttctccc atgtggcggc
     6001 gcagaatcaa atcgtgcaga caatggaggt gtttggaaac gaaaatgacc attcgcgacc
     6061 ggtaatcggt gaaatgcagc gtccatttca tgtcatactg gcgggccagg ggagcaaaca
     6121 cgacgggaat ttcttcacgg ctgagcgcaa aaccgttcag ctcccactcc atgcgcatga
     6181 aaaaatactc cccccgtttg gaggtgtatt gatccgctgc gacgatgttg ccgttgtttt
     6241 gaaaaataaa actggaaagt ctggccacca gccccttgcg atccgggcag gacaacaata
     6301 aaattgcatt ggattcatcc ataacattcc atctttcagc atcggagaaa taatgaagtt
     6361 gcttatagaa ggatagacgt tgcaggtcaa ggataaaaat ggtaagaacc cggaaccttg
     6421 caaagacgcg gaaaagagaa aaccatgaaa cgaatcgaga ccatccatct gcccaacggc
     6481 ctgatgctta cgatcaacga cctgtcgcgc cgcatcgccg cggacacggt aaaagttgaa
     6541 ctctcctttc agatgaaagt caaagtcgag ccgtcctttt tcgcttcgcc cgacgaccac
     6601 cggcagctta ccggcatctt cggcgacgag ctgacctacg agcacaaact ggaaagaacc
     6661 ttcgttgccg aagccgagaa gtcggccacc cgggcggacc ttctggaaac ctttaaaa
//
LOCUS       DAOSIM010000105         6716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_10325_length_6716_cov_7.317520, whole genome shotgun sequence.
ACCESSION   DAOSIM010000105 DAOSIM010000000
VERSION     DAOSIM010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10325_length_6716_cov_7.317520"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..762
                     /locus_tag="PLR47_05805"
     CDS             124..762
                     /locus_tag="PLR47_05805"
                     /inference="COORDINATES: protein motif:HMM:NF017114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbfB domain-containing protein"
                     /protein_id="HOG12493.1"
                     /translation="MKAHKRVPFGSLLACVLVAGFLFAVNASAAQVVVTYRDGNTQSF
                     TLNQTVAAVAKIEIGEGAAAAAVPAPGSTVSLQSVNYPTHYLRHRGFMGYLTPVTSPL
                     DRSDATFRIVPGLAGGDSISLESVNYPGYFLRHQGYQIKLHQASGDSLYRSDSSFRVK
                     PGLADRTKVSLESVNYPGYYLRHRGFQFYIEQGSGELYNLDCTFSVVEPLAR"
     gene            853..2088
                     /locus_tag="PLR47_05810"
     CDS             853..2088
                     /locus_tag="PLR47_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOG12494.1"
                     /translation="MKRKNTVGLWLGAAILILCLAAGAGLSQVSPTDELLEIVRTGWN
                     KDISRTQVLIDQGADVNAKNKYGDTPLVMAARMGSNKTVAILLARGADVNIPSRNGWT
                     PILHASQRIHNNNNPHLSVEIINALIDKGADVDAKTPGGETSLFLSAWRGDLVIVNNL
                     LARGANVNVRMKNGETPLIRVTHPTICKHRKEVIKALIAHGADVNAKTDLKLSAGYTP
                     LIQAAWGGATEVVALLLERGADVNAGLRDGQTPLYFAASQGKGFPEIVSLLLEKGADV
                     NAAAPGDYGKTALMAAANNGNTDIVRMLLDRGADLSLKSKENSRNALLYAAENGKTET
                     VRLLLDRGAVLNSKNGYGETSLMLAVTKNHTKTVELLLERGADPNIKARDRETPLIRA
                     SRNGNVEIVNLLKRAGAKP"
     gene            complement(2191..3384)
                     /locus_tag="PLR47_05815"
     CDS             complement(2191..3384)
                     /locus_tag="PLR47_05815"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HOG12495.1"
                     /translation="MASTTANDVLAVSGVADLNGLLKTVWLGSSVPKPNTSFASFLTA
                     ANIIGGFSNLSTAISPTLKFIPQYTADNKEVYLVTERDYENSSLQSSLSANQRAVSAM
                     LSPLANGAAGDLDTVFSTIDSLSTNDQVAAAFDSLMPVSGASQTTMSARAAVFQSGQA
                     AGRLEELRSGVQGFSFSGLELIDREFYHHNGRRPILLAAAGDDLRGMISREADPRWGV
                     FAKGSLILGDQKNTADQKGYDFKNGGFTLGMDYRIAPRFVAGAMFGYNDARSSVNDAG
                     SKIAMEGYAASLYGSVFGDALYGDFQLSYGWNRYDNTRRIVFPGLDRTAASSPSGSQI
                     SAYGGTGYDFRKGNWTLGPTLSLQYIRLQVGEYTETGAGALNLRVDQNTVKSILGSAG
                     VRLPF"
     gene            complement(3393..3956)
                     /locus_tag="PLR47_05820"
     CDS             complement(3393..3956)
                     /locus_tag="PLR47_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12496.1"
                     /translation="MVDVAPENTGTWLKYGVRFHLGKMELQKDGPVDFGAANVEAESL
                     TMTLYDNAAYKINDGYSFTVRDGLSLKKGTLEVNGTFDLQGAYDSVIDGKLTGTGAFY
                     KSGVGALTINGDASGFSGTTYIGAGRVKMDAGGVLGGNIEIDSGAVLGGTGRVGTIFA
                     KQGMVEPGNSIGTLRVAAITPRAHPAG"
     gene            complement(4114..5625)
                     /locus_tag="PLR47_05825"
     CDS             complement(4114..5625)
                     /locus_tag="PLR47_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOG12497.1"
                     /translation="MNQRFFFTRTLSILTTGVVFCAAILLCAPCLYAQGAAFIPSQEK
                     DPCFFPLTSGGKTYPGQWHLWNQMPVKADLNAGLDAGLKDAWNDGSGLGYTGRGVVIG
                     IVDDGVEGTHEDIKDNYLPDLSRNFSQDASMAAGEQGPVQQGDNHGTAVAGVAAARGG
                     NNIGGVGAAPYAGIAGMRIRLGDSYTGDPAVTEQDYYDVHLWKSGLNPSTLAVEAAPQ
                     IHVKNHSYGPSTPFEEHPDRLKDIMRAAAGNGVVSVYAAGNARNKTEKKKEASCEDAN
                     KDFTLTSPYVLTVAAMGSDGTYASYSSYGANVFVTAPSSSWLEYFSITTADRTGTDYG
                     YNKYDAEKNPKGDDRETFPNTNYTSTFGGTSSAAPLVSGITALGKEANPLMSIRMAKH
                     VLSLPSVTFKNDAANSEWILNKATDVSRNFNPNYGFGLIQADKFVGKIEEVAYITTEK
                     VVDIDATDVNKSIPDNDPAGRSLTFTINPALPVESVEVASDFPTSAGAISGPI"
     gene            complement(5634..>6716)
                     /locus_tag="PLR47_05830"
     CDS             complement(5634..>6716)
                     /locus_tag="PLR47_05830"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG12498.1"
                     /translation="ALAAPWLLKPLWAPLVDRYWSGKIGRRRSWIIPLQTFMILIMLA
                     SVPAAASGAVTPLLACIFLMNLCAATQDIAVDGLAVDILKQEELGPGNAAQVAGYKMG
                     MMLGGGILVWLAGRTGWGGLFLTMAAVAALPLILILRHREADPGGPDGAARPKPSDFL
                     HALAAALRSPGAIPFLVFLATYKTGETMIDMMFKPFLVDCGFGAQQIGLWVGTWGMGA
                     SLLGSVFGGWLATRLPIVKALFAAASLRLIPLVAELWLSTISPAASQVAAVTVAEHFF
                     GGMLTTALFAYMMSRVDKRIGATHYTILAAIEVLGKSPGSWASGWLAERFGYTGLFSL
                     GVVLSVLVLFLYFPLRRQNKLQQAEV"
BASE COUNT         1374 a         1970 c         2042 g         1330 t
ORIGIN      
        1 ggggcaccaa ttcacatcaa cggtttgtac aacatacatg gtcatttgta tttagaggaa
       61 tcgtcatata cggggcatcc ccgggattgt tttttcaacc aaaccttacg gaggaacaga
      121 ttgatgaaag cacacaagcg cgtacccttt ggctccctgc tcgcctgtgt tctggtggcg
      181 gggtttcttt ttgcggtgaa tgcatcggcg gcgcaggtcg tggtgaccta ccgcgacggc
      241 aacacgcagt cgttcacctt gaatcagacc gtggccgcgg tcgccaagat cgaaatcggc
      301 gagggcgcag ccgcagccgc cgtgccggcc cccggcagca ccgtctccct gcaatcggtg
      361 aattacccta ctcattattt gaggcatcgg ggcttcatgg gctacctgac cccggtgacc
      421 tcaccgctgg accgcagcga tgccaccttc cgaattgttc cgggcctggc cggcggggat
      481 tcgatttctc tcgaatccgt caattatccg ggatacttcc tgcgccacca agggtatcag
      541 atcaagttgc accaggccag cggagactcg ctctaccggt ccgattccag tttccgggtc
      601 aaaccgggac tcgcggaccg gaccaaggtt tccctggaat cggtcaatta tcccggatat
      661 tatctgaggc accgcggctt tcaattctac atcgagcaag gcagcggtga actttacaac
      721 cttgactgca ccttctcggt tgtcgagcct ctggcccggt agtccggctt cccggagcgc
      781 tctgggcgat cccgaaaggc ccctgagaga tcctgcatat cgatggcagt gcgccgaagg
      841 gagggtcgtt tgatgaagag aaaaaatacg gtgggtttgt ggctcggcgc ggcgattctg
      901 atcctgtgtc ttgcggccgg cgccgggctt tcccaggtat cgccgactga cgaactgctc
      961 gagatcgtcc gcacaggctg gaacaaggac atcagcagaa ctcaggttct gatcgaccag
     1021 ggtgcggatg tcaatgccaa aaacaaatac ggggacacgc cgttggttat ggccgccaga
     1081 atgggcagta ataagacggt cgcgatcctt ttggccaggg gtgccgatgt caacatcccc
     1141 tcccggaacg gatggacgcc gatcctccat gcctcgcagc gaatccacaa caacaacaat
     1201 cctcacctga gcgtcgaaat cattaacgcc ctgatcgaca aaggagcaga tgtcgatgcc
     1261 aaaacgcccg ggggtgaaac atcgcttttc ctcagcgcct ggcgcggaga cctcgtcatc
     1321 gtcaacaacc tgctggccag gggtgcgaac gtcaacgtca ggatgaaaaa cggcgaaacg
     1381 cccctcatcc gagtgacgca tccaaccatc tgcaagcatc gaaaagaggt catcaaggcg
     1441 ttgattgccc acggagccga cgttaatgcc aaaaccgatt tgaaattgag cgccggttac
     1501 actccgctga tccaggcggc ctggggcggc gccacggaag tcgtcgcact gttgctggaa
     1561 aggggagcgg acgtgaatgc cggtttgcgc gacggccaga cccctctgta ttttgctgcc
     1621 agtcagggga agggatttcc ggaaatcgtc tcgctgttgc tggaaaaggg agcggacgtg
     1681 aatgccgcag cgccgggaga ctacggcaag acggccctga tggcagctgc gaataacggc
     1741 aatacggaca tcgtcaggat gctgctggac cgcggcgccg atctgtccct gaagtcgaag
     1801 gagaattcca gaaacgccct gctgtatgcg gcggaaaacg gcaagaccga aacggtccgg
     1861 ttgttgctgg accgcggcgc cgtcctgaat tcaaaaaacg gttacgggga aacctccctg
     1921 atgctggccg tcacgaaaaa tcataccaag acggtagagc tgctcctgga gcggggggcc
     1981 gatcccaata ttaaggcccg ggatcgggaa acgccgctga tccgcgcctc gcgcaacggc
     2041 aacgtcgaga tcgtcaacct gctcaaacgg gctggggcga aaccctgagc ggaaggccga
     2101 tgaaaccgca ctgcagggaa caatggtttc atgtttccgg cagagggact cacgctggtt
     2161 gaaccggcgg gccgctatcg ccgcaaccgc ttaaaacggc agcctcaccc cggcgctgcc
     2221 cagaatcgac ttcactgtgt tctggtccac gcgcaggtta agggcgccgg cgccggtttc
     2281 cgtgtattcc cccacctgga ggcggatgta ctggagcgac agcgtaggcc ccagcgtcca
     2341 gtttcctttc cgaaagtcgt agcccgttcc gccgtaggcg gagatctggc ttccggaagg
     2401 gctggaagcg gccgttcgat ccaggccggg gaagacaatc cggcgggtgt tgtcgtagcg
     2461 gttccagccg tagctcaact gaaaatctcc gtatagcgcg tcgccgaaaa cggatccgta
     2521 aaggctggcg gcgtaccctt ccatcgcaat tttgctgccg gcatcgttta cggaggaccg
     2581 ggcgtcgtta taaccgaaca tcgccccggc aacaaagcgg ggagcgatgc gatagtccat
     2641 gcccagcgta aagccgccgt ttttgaaatc atagcccttc tgatccgccg tgttcttttg
     2701 atcccccagg atcagacttc cctttgcaaa aacgccccac cgcggatctg cttcgcggga
     2761 gatcatcccc cggaggtcgt ctccggcggc ggccagcagg atgggacgcc ggccgttgtg
     2821 atgataaaat tcccggtcga tgagttccag accggaaaaa ctgaagccct ggactcccga
     2881 ccgcagctct tccaggcggc cggcggcctg gccggactga aagacggcgg cgcgggcgga
     2941 catggtcgtc tgggacgcgc cgctgacggg catgagggaa tcgaaagcgg cagccacctg
     3001 atcgttggtg gaaagagaat caatcgtgct gaaaaccgta tccaggtctc cggcggcgcc
     3061 attggccagg ggactgagca tcgcgctcac cgcccgctgg ttggcactca gggaagactg
     3121 gagagaggaa ttttcgtagt cgcgctccgt gacgagatac acctctttat tgtccgcggt
     3181 gtactgcggg atgaatttca gcgtcgggct gatggccgtg gacaagttgg aaaatccgcc
     3241 gatgatgttg gcggcggtga gaaaactggc aaagctggtg ttgggcttgg gcacggagct
     3301 tcccagccag acggttttca gcaaaccgtt gagatccgcc acgccgctga cggcgagaac
     3361 atcgtttgcg gtagtcgagg ccacttcgat ggttagcccg ccggatgcgc tctgggtgta
     3421 attgccgcca ccctcagggt gccgatggaa ttgccgggct ccaccatgcc ctgtttggcg
     3481 aagatcgttc ccactctgcc tgtgccgccg aggacggcgc cgctgtcgat ttcgatgttt
     3541 ccgcccagga cgccgcccgc gtccatcttc acacgtcctg cgccgatgta cgtggttccg
     3601 gaaaacccgg aggcgtcgcc gttgattgtc agggcgccca ctccggactt gtagaaagcg
     3661 ccagtcccgg taagcttccc gtcgatgacg gaatcatagg ccccctgcag atcgaaagtg
     3721 ccgttgacct ccagtgtgcc ctttttgagg ctgagaccgt cacggacggt gaaagaatag
     3781 ccgtcgttga tcttgtaggc ggcgttgtcg tacagagtca tcgtcaggga ttccgcctcg
     3841 acgttggcgg ctccgaaatc gaccggtccg tccttctgca gctccatctt tcccagatga
     3901 aaacggacgc cgtacttaag ccaggtgccg gtgttctccg gcgcgacatc gaccatctta
     3961 agcgtccatg cgtcgctttt ttgttctcct cagaggcgtt ggtaagaaag gtccagccga
     4021 aatccgtgac cgcttcattg tcctggtata atggattatt gatggttttg ctcgtgtcgt
     4081 tgaagaggcg gctctctgtc ccatcgggcg aaatcagata ggcccggaga tcgccccggc
     4141 gctggtggga aaatctgagg ccacctcgac gctttcgacg ggcagggcgg ggttgatggt
     4201 aaaggttaag gatcgtccgg caggatcatt gtcgggaatg gacttgttga cgtccgtggc
     4261 gtcgatgtcc acgaccttct ccgtcgtgat gtacgccact tcttcgatct tgcctacgaa
     4321 tttgtcggcc tggatcagcc cgaagccgta gttggggttg aagtttctcg aaacgtccgt
     4381 tgctttattc aaaatccatt cgctgttggc cgcgtcattt ttgaaagtga cggacggcag
     4441 ggaaaggacg tgcttggcca tgcggatgga catcagcggg ttggcctcct tgcccagcgc
     4501 cgtgattccg gaaaccagcg gggcggcgga ggacgtgccg ccgaatgtgc tggtgtagtt
     4561 cgtattggga aatgtttcgc ggtcgtcgcc tttggggttt ttttcggcat catatttgtt
     4621 gtagccgtaa tcagtgccgg tccggtccgc cgtcgtgatg ctgaaatatt ccagccagga
     4681 gctcgacggt gcggtaacaa agacattggc cccgtaactg ctgtaggagg cataggttcc
     4741 gtcgcttccc atggcggcca cggtcaggac gtaaggcgag gtgagcgtga agtccttgtt
     4801 ggcatcctcg cagcttgcct ccttcttttt ctcagtcttg ttgcgggcgt ttcccgcggc
     4861 atacacactg acgacgccgt ttcccgccgc ggccctcatg atatccttca accggtcggg
     4921 atgctcctca aagggcgtcg aaggaccgta actgtggttt ttgacatgaa tctgaggcgc
     4981 ggcttcaacg gccagcgtcg aggggttgag accgctcttc cagagatgca cgtcataata
     5041 gtcctgttca gtcactgccg gatcgccggt gtaggagtcg ccgaggcgga tgcgcatacc
     5101 ggcaataccg gcgtaagggg ccgcgcccac gccgccgata ttatttcctc cccgggcggc
     5161 ggccacaccc gccacggccg tgccgtggtt gtcgccctgc tgaaccggcc cctgttctcc
     5221 ggcggccatt gatgcatcct gactgaaatt cctgctcaga tcaggcaggt agttatcttt
     5281 gatgtcctcg tgggttccct ccacaccgtc gtcaacgata ccgatgacga cgcctctccc
     5341 cgtgtagccc agaccgcttc cgtcattcca ggcgtctttc agccccgcgt cgaggccggc
     5401 attcaagtca gccttcacgg gcatttgatt ccaaaggtgc cattggcccg gataggtttt
     5461 gccgccgctg gtcaggggga aaaaacaggg gtctttttcc tgcgagggaa taaaggccgc
     5521 cccctgcgca taaagacagg gggcgcaaag aagaattgct gcacaaaaaa caactcccgt
     5581 ggtcaggatg gacagggtcc tggtgaagaa aaagcgctga ttcattgaaa tgatcaaacc
     5641 tccgcttgct gtagtttgtt ttgccggcga agcgggaaat aaagaaacag caccaggacc
     5701 gacaggacga cgcccagtga aaacagtccc gtatagccga agcgctcggc cagccatccg
     5761 gaagcccagc tgccgggtga ctttcccagg acttcgatgg ccgcaagaat tgtgtaatgc
     5821 gtggccccga tccgtttgtc cacgcgggac atcatgtacg cgaaaagagc cgtggtcagc
     5881 atgccgccga agaagtgctc cgcaacggtc acggctgcca cctgcgatgc ggcgggcgaa
     5941 atcgtggaaa gccacagctc ggccacaagc gggatcaacc gaagcgaggc ggcggcaaag
     6001 agagccttca cgatgggaag gcgcgttgcc agccagcctc cgaagaccga tccgagaagg
     6061 gatgcgccca ttccccaggt gcccacccac aggccgatct gctgggcccc aaagccgcag
     6121 tccaccagga aaggcttgaa catcatgtcg atcatggtct cccctgtctt gtaggtcgcg
     6181 aggaagacga ggaaaggaat ggctccgggg gagcgcagag cggccgcaag cgcgtggagg
     6241 aaatccgaag gcttcggccg ggcggctccg tccggaccgc ccggatcggc ctcccgatgg
     6301 cgaagaatca aaatcagcgg cagcgccgcc accgccgcca tggtcagaaa aagacctccc
     6361 cagcctgtgc gtccggcgag ccacactaaa atgccgccgc ccagcatcat gcccatcttg
     6421 tagcccgcga cctgcgcggc gttgcccggg ccgagttcct cctgtttcag aatgtccacc
     6481 gccaggccgt ccacggcgat gtcctgcgtg gcggcgcaaa ggttcatgag gaagatgcat
     6541 gccagaagcg gcgtcacggc gccggaagca gccgccggaa cggacgccag catgatcagg
     6601 atcatgaagg tctgcagcgg gatgatccag gaacgtcggc gtccgatctt gcccgaccag
     6661 tagcggtcca ccagcggcgc ccacagcggc ttgagcagcc agggcgcggc cagcgc
//
LOCUS       DAOSIM010000106         6709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_10346_length_6709_cov_6.791855, whole genome shotgun sequence.
ACCESSION   DAOSIM010000106 DAOSIM010000000
VERSION     DAOSIM010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10346_length_6709_cov_6.791855"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PLR47_05835"
     CDS             <1..252
                     /locus_tag="PLR47_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12499.1"
                     /translation="EMERNFKIDDRILKFMTVTREGAVTREGIDAEIAASEVKRTQIR
                     IETDNTAAAKEEKAAPDAKPSRLSFRKAQPREDNTPKGE"
     gene            255..524
                     /gene="rpsR"
                     /locus_tag="PLR47_05840"
     CDS             255..524
                     /gene="rpsR"
                     /locus_tag="PLR47_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOG12500.1"
                     /translation="MAAPNGERPSRPPQKKFFHRKKFCRFCTDSNVKIDYKNPVILNN
                     FVTERGKIMPRRITGNCAYHQRELTVAIKRARTIAIMPFVTAEGR"
     gene            642..1607
                     /locus_tag="PLR47_05845"
     CDS             642..1607
                     /locus_tag="PLR47_05845"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HOG12501.1"
                     /translation="MTTLNLSPFHDASFPRLLLVIALFLAASLLPFAGLVFLLVIPLV
                     LYVLCFLSDPAKTLKAFLASLCAVAALLWTMNAAASVAAPAAMGIAGMLMAWSAEKRF
                     RVEAVVLLPALVVLGAIAFYFIFGALQQSLTPWELVLKNIRESVELNIEFYSRLPLNP
                     EELQAIVDSKPQMISLFARIFPALCVMAAVFTIWVNLIAAGRLLKSRGIVLPQLSNLS
                     QWSAPGWLVWVFIAAGGLGAVPVTPIRVVGVNILLVASLLYFLQGLAIVSFFFQHKNI
                     SPFFRFLAYFLIAIQQILMIAIAAVGFFNLWVDFRKYLRKDRSTD"
     gene            1642..2091
                     /gene="rplI"
                     /locus_tag="PLR47_05850"
     CDS             1642..2091
                     /gene="rplI"
                     /locus_tag="PLR47_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOG12502.1"
                     /translation="MKVILKQNVPSLGKAGDLVKVNDGYARNLLIPKGLAVEADDKNL
                     KAFELAKKNILQKTQKEKAGAQDLASRLSQVTLTLSRKVGDQHKIFGSVTSKDLEAAI
                     REKGFPVDRKMIVHDEPIKSLGEFKVRIKLHPGVETEIQVHVVGEES"
     gene            2088..3446
                     /gene="dnaB"
                     /locus_tag="PLR47_05855"
     CDS             2088..3446
                     /gene="dnaB"
                     /locus_tag="PLR47_05855"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOG12503.1"
                     /translation="MKDIDPSLYKLPPQNIEAEQSILGSILLENGTINAVQEILNRDD
                     FYSEAHRKIFSAIERMADKNEPVDLITLSSALKDQNLLDSIGGAAYLASLVDNVPTAA
                     NAANYAKIVKGKSVLRGLIGSATEIINSCYDTGSDVDQVLDIAEHRIFEISENKVRAS
                     FYPIKDIVRDTFRSIEDLFTRKELITGVPTGFDKIDDMTSGLQNSDLIIVAGRPSMGK
                     TALALNVAQHAALETQIPVAIFSLEMSKEQLAFRMLASEARVDSQRMRKGFLGETDWP
                     KLTAAAGRLSEAPLFIDDTPAITVLEMKAKSRRLKAERDLGLIVVDYIQLMRSSGNTN
                     SREQEISEISRSLKALAKELKVPVIALSQLNRQVETRPGGNKRPQMSDLRESGAIEQD
                     ADVIIFIYRDEVYNKSDDNPEKGTAEIIIGKQRNGPTGPVKLAFLDRFTTFENLARTD
                     EP"
     gene            3443..3955
                     /locus_tag="PLR47_05860"
     CDS             3443..3955
                     /locus_tag="PLR47_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12504.1"
                     /translation="MKRNPVEVIVCDRMQKNYRYFLTKPAGKDFDPEFKPELTPRQML
                     EIGVFGGKYMTDCRGEFPPSWFAKARLSPERHDPKLNCFGVNASKPLSYWVAKGWIYP
                     EDPRGWFQWYCRYFLGRRSPDDTRQIKRWKAMQRHVSQLILHCPAGDLSCRRRQRQAL
                     LHWAYDSRKM"
     gene            complement(4065..6503)
                     /locus_tag="PLR47_05865"
     CDS             complement(4065..6503)
                     /locus_tag="PLR47_05865"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOG12505.1"
                     /translation="MFTNKRSIWTFLLSGLMVLLAAGAAFAGEADINLPDLAQVTFVG
                     GTLSGMTILNIGLFICLIGMAFGVMQYVQTKNLPAHKAMLDVSQTIWETCKTYLFQQG
                     KFLIALWALIAICMIYYFGALQSKPVSDILIILICSIVGILGSYGVAWFGIRINTVAN
                     SRAAFSSMSGEPLNIVNICLRSGMSVGLLLVSIELFFMLIILAYIPKELAGPSFVGFA
                     IGESLGASALRICGGIFTKIADIGSDLMKIVFHLPEDDPKNPGVIADCTGDNAGDSVG
                     PTADGFETYGVTGVALITFLALALAGNPEMGGKLIIWIFAMRILMILTALLSYFVNDG
                     ISKAAFGGKKEFNFEHPLTNLVWITSILCIIVTFVASYYLLGDLTEPYTSLWWALSII
                     ISCGNLAGALIPEFTKIFTSTSSRHCEETVNASKQGGPSLNILSGLVAGNFSGFWLGL
                     VIAGLMYVAYLVSGVASVIALMPPAFTFAAPVFAFGLVAFGFMGMGPVTIAVDSFGPV
                     SDNAQSIYELSMIESRPDAKAVVKKEYGKEPDFELAKHFLESADGAGNTFKATAKPVL
                     IGTAVVGATTMVFGIIIMLEGIFGNVIQNLSLVQPDIIVGLILGGCIIYWFTGASIQA
                     VTTGSYQAVVYIKKNINLDKKEASIEDSKKVVQICTQYAQKGMINIFIVIFFMCLALA
                     FFNPYFFIGYLFGIAFFGLFQAVFMANAGGCWDNAKKIVEVDLKMKNTPLHEASVVGD
                     TVGDPFKDTSSVSLNPVIKFTTLFGLLATEIAVTMTNVNLKYALSAIFFVIALIFVYR
                     SFYSMRIASEKLGG"
     gene            complement(6507..>6709)
                     /locus_tag="PLR47_05870"
     CDS             complement(6507..>6709)
                     /locus_tag="PLR47_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12506.1"
                     /translation="LGIGKPVDKSRIEGYVLEPFRKEEQEILPEVIERAADASAEIVL
                     NGLQQAIGMYQTKNMNFLKKED"
BASE COUNT         1678 a         1866 c         1748 g         1417 t
ORIGIN      
        1 gaaatggaga gaaattttaa aatcgacgac cggattctga aattcatgac ggtcaccagg
       61 gaaggcgccg taacccgcga aggcatcgac gcggaaatcg ccgcctcgga agtgaagcgc
      121 acacagatcc gcatcgaaac ggacaacaca gccgccgcga aagaagaaaa ggcggccccg
      181 gatgcaaagc cctcccggct gtccttccgc aaagcccagc cccgggaaga caatacaccg
      241 aaaggagaat gatcatggct gcacccaacg gcgaaagacc ctcccgtccg cctcagaaaa
      301 agtttttcca ccggaaaaaa ttctgccggt tctgcacgga ctccaatgtg aagattgatt
      361 ataaaaatcc ggtgattctc aataattttg tcacggagcg cggcaagatc atgccccgga
      421 gaatcactgg caactgcgct tatcatcaga gagaactgac cgttgccatt aaaagagcgc
      481 gcaccatcgc catcatgcct tttgtcaccg cggaaggcag ataaaccaat tttgcaaagc
      541 caccggccgg ttttattctg atgaagaaaa aaccggccgg ttttcattga tccgacacat
      601 cctttgacgg cattgccgtt ttttcaccgc aggcccctga tttgacaaca ttgaatctat
      661 cgccttttca tgatgcttcg tttccccggc tgctgctggt catagcgctt tttctggcgg
      721 catcgctgct cccctttgcc ggcctcgttt tcctgctcgt gattccgctg gttctgtatg
      781 tcctgtgctt tctttccgat cccgcaaaga ccctcaaggc atttctggcc tcgctttgcg
      841 ccgtggccgc cctcctgtgg accatgaatg cggccgcatc ggtcgcggcc cctgcggcca
      901 tggggatcgc gggaatgctg atggcctgga gcgccgaaaa acggtttcgt gtcgaagccg
      961 ttgtacttct gcccgcgctc gtcgtgcttg gcgcaatcgc cttttatttt attttcggcg
     1021 cgttgcagca gtcgctcacc ccctgggagc tggttttaaa gaacattcgg gaatcggtgg
     1081 aattaaacat cgagttttac agtcgattgc ctctgaatcc cgaagaactt caagcgattg
     1141 tggacagcaa gccccaaatg atatccctgt ttgcgcggat tttccccgcc ctttgcgtca
     1201 tggcggccgt attcacgatc tgggtgaatc tgatagccgc gggcaggctg ctcaaaagcc
     1261 gcggcattgt actgcctcag ctttcgaacc tgtcccaatg gagcgcgccc ggctggctgg
     1321 tctgggtttt catcgcggcg ggagggctcg gcgccgtccc cgtcaccccc atccgcgtcg
     1381 tcggcgtcaa catccttctc gtcgcgtctt tgctatattt tctgcaaggt cttgctattg
     1441 ttagcttctt ttttcagcat aaaaacatct caccgttttt ccgcttcctt gcttattttt
     1501 taattgcgat tcagcaaatt cttatgatag caatagcggc agtgggattc tttaatctct
     1561 gggttgattt tcgaaaatat ctgcgcaaag accggtcgac agactgacca ttgcattcca
     1621 tacgagacag gaggtttgac catgaaggtg attttgaagc aaaacgtccc ttctctgggc
     1681 aaggccggag acctggtcaa ggtcaacgac ggctacgccc gcaatctgct gattccgaag
     1741 ggtctggccg ttgaggcgga cgacaagaat ctcaaggcct ttgaactggc caaaaaaaat
     1801 attctgcaaa agacgcaaaa ggaaaaggcc ggcgcgcagg atctcgcttc ccgtctgagc
     1861 caggtgacgc tgacgctttc ccgcaaagtg ggagatcagc acaagatttt cggctccgtt
     1921 acatccaagg acctggaagc ggcgatcagg gaaaagggat ttcctgtgga ccgcaaaatg
     1981 attgtgcacg atgagccgat caaatcactg ggagagttca aggttcgcat caagctccac
     2041 ccgggcgttg aaacggaaat ccaggtccat gtggtcggcg aagaatcatg aaagacattg
     2101 atccctctct atacaaactg ccgccccaga atatcgaggc cgaacagtcc atcctgggca
     2161 gcattctgct ggaaaacggc accatcaacg cggtgcagga aatcctgaac agggatgact
     2221 tctacagcga agctcaccgg aaaatttttt ccgcaatcga gcgaatggcg gacaagaatg
     2281 agccggtgga cctgattacg ctcagcagcg cgctgaagga ccagaatttg ctggattcga
     2341 taggcggcgc ggcctacctg gcatcgctgg tggacaacgt tcccacggcc gccaacgcgg
     2401 ccaattacgc gaagatcgtc aagggaaagt cggttctgcg cggcctgatc gggtccgcca
     2461 ccgaaatcat caacagttgc tatgacacgg gctccgacgt cgaccaggtg ctggatatcg
     2521 ccgagcaccg cattttcgaa atttccgaaa acaaggtgcg ggcctctttt tacccgatca
     2581 aggacatcgt ccgcgacacc ttccgctcca tcgaggacct gttcacccgc aaggagctga
     2641 tcaccggcgt tcccaccggc ttcgacaaaa tcgacgacat gacctccggg ctgcagaatt
     2701 ccgatctgat tattgtcgcc ggccgcccca gcatgggcaa gaccgcctta gcgctcaatg
     2761 tcgcacagca cgccgctctg gaaacccaga ttcctgttgc cattttctcg ctggaaatgt
     2821 ccaaggagca actggccttc agaatgctgg cgtccgaagc ccgtgttgac tctcagcgca
     2881 tgcgcaaggg ttttctgggg gaaaccgact ggccgaagct gacggcggcg gcgggccggc
     2941 tctccgaagc gccgctgttt atcgacgaca cgcccgccat caccgttttg gagatgaagg
     3001 caaagtcccg ccgcctgaaa gcggagcggg acctggggct gatcgtcgtg gactacattc
     3061 agttgatgcg gtccagcggc aataccaatt cgcgcgagca ggaaatttcc gagatcagcc
     3121 gctcgctcaa ggcgctggcc aaggagctca aggtgcccgt catcgcgctt tcccagctca
     3181 accgccaggt cgagacaagg ccgggcggca acaagcgtcc tcagatgtcg gacctgcgcg
     3241 aatccggcgc catcgaacag gacgcggacg tgatcatctt catctaccgg gatgaggtgt
     3301 acaacaaatc cgatgacaac ccggaaaaag gcaccgccga aatcatcatc ggcaagcagc
     3361 gcaacggccc caccggtccg gtgaagcttg cgttcctgga ccggttcacc acctttgaga
     3421 acctggcccg gacggacgag ccatgaaaag aaatcccgta gaagtgatcg tttgcgaccg
     3481 gatgcagaaa aattaccgct attttctgac caaaccggcc ggaaaagatt ttgacccgga
     3541 attcaagccg gaactgaccc ccagacaaat gctggaaatc ggcgttttcg gcggaaaata
     3601 catgacggac tgccgcgggg aattcccgcc ctcgtggttt gccaaagcca ggctctcacc
     3661 cgaacgccac gatccaaagc tgaactgctt cggcgtaaac gcgtccaagc ccctctccta
     3721 ctgggtggcc aaaggctgga tttatcctga agatccccgc ggctggtttc aatggtactg
     3781 ccgctacttt ctgggccgcc gctctcccga cgacacccgg caaatcaaac gctggaaggc
     3841 catgcaaagg cacgtctccc agctcatctt gcactgcccg gcgggcgacc tttcctgccg
     3901 ccgcagacaa cgccaggccc tgctgcactg ggcatacgac agccgcaaaa tgtaaacccc
     3961 ctgaacaccg atccgatgct ctttttcgcc ttccggcaaa cctcgcgcaa aaaaagggcg
     4021 ctcccttgcg gggagcgccc ttgacggtta cagggtttgc tggattatcc gcccagtttt
     4081 tcgctggcga ttctcatgga gtagaaggag cggtacacga aaatcagagc gatgacaaag
     4141 aagatagccg acagagcata cttcaaattg acgttcgtca tggtcaccgc gatttccgtg
     4201 gccagaagac cgaacagcgt cgtgaatttg atgaccgggt tcaaggaaac cgaagaggtg
     4261 tctttgaagg gatcgccgac ggtgtcgccg accacgctgg cttcgtgcag cggagtgttc
     4321 ttcatcttga ggtccacttc aacaatcttc ttggcgttgt cccagcaacc gccggcgttg
     4381 gccatgaaga ccgcctggaa cagaccgaag aaggcgatgc cgaacagata accgatgaag
     4441 aagtaggggt taaaaaaggc gagcgccaga cacatgaaga agatcacgat gaagatgttg
     4501 atcataccct tctgtgcata ctgagtacaa atctgaacga ccttcttgga gtcttcaatc
     4561 gaagcttcct tcttgtccag attaatattc ttcttaatgt acaccacggc ctgataagaa
     4621 ccggtggtca cggcctggat ggaagcgccg gtaaaccagt aaatgatgca accgcccaga
     4681 atcaagccca caatgatatc cggctggacc agagaaagat tttgaataac attgccgaag
     4741 ataccttcca gcatgatgat gatgccgaac accatggtgg tggcgccgac aacggcggtg
     4801 ccgatcaaca caggcttggc tgtcgcttta aaggtattgc ccgcgccgtc ggccgattcc
     4861 aggaagtgct tggccagttc gaagtcaggt tctttcccgt attctttttt cacaacggct
     4921 tttgcatcag gacgcgattc aatcatcgac agttcgtaaa tggactgagc gttgtccgaa
     4981 acaggaccaa agctgtccac agcgatggtc acgggaccca tgcccatgaa accgaaagcc
     5041 accagaccga aggcaaacac cggcgccgca aaggtaaagg ccggaggcat cagagctatg
     5101 acagatgcaa cacccgagac caggtaagcg acatacatca aacccgcaat aaccaggccc
     5161 agccagaaac cggagaagtt gccggcaacc aatcccgaaa gaatgttcag ggaaggaccg
     5221 ccctgtttgg aagcattaac ggtttcctcg cagtgacggg aagaggtgct ggtgaatatc
     5281 ttggtgaatt ccggaatcaa cgcaccggcg aggtttccgc agctgatgat gatggacagc
     5341 gcccaccaca gactggtgta aggttcagtc aagtcaccca ggaggtaata actggcaaca
     5401 aatgttacga tgatgcacag gatggaggta atccatacca gattggtcag gggatgttca
     5461 aagttaaatt ctttcttgcc accaaaagca gctttgctga taccgtcatt aacgaaataa
     5521 gaaagaagag ccgtcaggat cataaggatt ctcatggcga aaatccagat aatcagtttg
     5581 ccgcccattt cgggattgcc cgccagagcc agggccagga aggtaatcag cgcgacgccg
     5641 gtaacgccgt aagtttcaaa accatcagcg gtaggaccaa cgctgtcacc cgcgttgtcg
     5701 cccgtacagt cggcgataac acccgggttt ttcggatcgt cttccggaag atggaaaacg
     5761 attttcatca agtcggaacc gatgtcggca atcttcgtga aaatgccgcc gcagatgcgc
     5821 agggcggaag cgcccagtga ttcaccgatg gcaaaaccga cgaagctggg accggccaat
     5881 tccttcggaa tgtaagccag aatgatgagc atgaagaaca attcgatgct gaccagcaga
     5941 aggccgacgc tcataccgga acgcagacag atgttcacga tgtttaaagg ctcgccgctc
     6001 atggaagaaa aagcggcgcg ggagttggcc acggtgttga tccggatgcc gaaccatgcc
     6061 acaccataag agcccagaat acctacaatc gagcagatca gaatgataag aatatctgat
     6121 acaggttttg actgcaaagc gccaaagtaa tagatcatac aaatggcgat cagagcccac
     6181 aaagcgatca ggaacttgcc ctgctggaaa agataggtct tgcaggtttc ccaaatggtc
     6241 tgggaaacat ccagcatggc cttgtgcgcc ggcagattct tggtttgcac atactgcatc
     6301 acaccgaaag ccataccgat caggcagata aacaatccaa tattaaggat tgtcattccc
     6361 gacagggttc cgcccacaaa tgtaacttgg gccaaatccg gcaaattgat gtccgcttca
     6421 ccggcaaatg ccgcaccggc ggcgagcagg accatcaatc cgctcagtaa gaatgtccag
     6481 atacttcttt tgtttgtaaa catcaactaa tcctcctttt taagaaaatt catatttttt
     6541 gtctggtaca tgcctatggc ttgctgcaga ccatttaaaa caatttcagc gcttgcatcg
     6601 gcggcccttt cgatgacttc gggcaggatc tcctgttctt cctttcgaaa aggctccaac
     6661 acataaccct ctattcggga tttgtcaact ggtttgccaa tacccagcc
//
LOCUS       DAOSIM010000107         6670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_10419_length_6670_cov_8.000756, whole genome shotgun sequence.
ACCESSION   DAOSIM010000107 DAOSIM010000000
VERSION     DAOSIM010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10419_length_6670_cov_8.000756"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PLR47_05875"
     CDS             complement(<1..783)
                     /locus_tag="PLR47_05875"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOG12507.1"
                     /translation="MQLNMTDDRLKNITLQQMEALIYLVEERSFSRAAARMLLTQPAL
                     TKQIRHVEDCLGVKAVNRSRDGVSLTPEGKILYDAVRRIVRLRRDAADKIDQLRKQPG
                     GDVYLSASTIPATYILPRAVSSFKKKHPDIRIFLKTEDSEEVINQVLDREVEIGCIGK
                     SPQNAKLIAEPVWHDRLILAVPAGHRWTKKGSVSVSELMEEPFVLREKGSATRDVFES
                     YMKEQQAVRLADFNICGELGSSEAIKEAVLAGLGVSVLSVHAV"
     gene            950..>1228
                     /locus_tag="PLR47_05880"
     CDS             950..>1228
                     /locus_tag="PLR47_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG12508.1"
                     /translation="MKLTDMFSLKGKVALVTGGGRGIGKFIATGLAEAGADIILTSRK
                     IKNLEATAAELEANQKVKAVAIACDMAKEDTVEAMLKEAVAKFGRIDIL"
     assembly_gap    1229..1348
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1349..1775
                     /locus_tag="PLR47_05885"
     CDS             <1349..1775
                     /locus_tag="PLR47_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG12509.1"
                     /translation="RGVWVLTQKVARIMKEQGGGNIINISSVMAFRGSIEEAHPAVPY
                     NSTKAAINLLTMNLAVKLAPHKIRVNAIAPGFFHTDMMAYIDKPEFKPVRDAIINEIP
                     LRRVGEIDDMKGVAVFLASDASQFITGHVMVVDGGQIAK"
     gene            1793..2680
                     /locus_tag="PLR47_05890"
     CDS             1793..2680
                     /locus_tag="PLR47_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12510.1"
                     /translation="MPASGQPAAPKESATVILVCSHPADAWNVFLARRHSRSSFMAEA
                     YVFPGGQIADADRDEDLRNFISMPPGFDPRAHLKDDSLSQETAQSLFVCAIRETFEET
                     GVLIARLRDGSPLQLTSKEQNARLAAYRGELNAGRTTLSEMARRENLVFPLDLLVPYA
                     HWITPEFLPKRFSTRFFLAALPEDQSAATDQGELTDSLWAKPEEVLRRYRGREIVLMP
                     PTLKTLEELARYACIDDLFAGVRSRAIYPILPEPGENMIKLPHDPEYGIERYKQPSRP
                     EEPSRIIMTDGIWRTGGKL"
     gene            complement(2802..3374)
                     /locus_tag="PLR47_05895"
     CDS             complement(2802..3374)
                     /locus_tag="PLR47_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOG12511.1"
                     /translation="MSAGKKIIIVVAVIAVLAISLYSLFAGNYNRFVRMDVAIKSAWA
                     QVENQLQRRYDLIPNLVETVKGYAKQEKDVLVEVTNARSKVGGAGTVPDKIAANNELT
                     GALSRLMVVVERYPDLKSNQNFLQLQDELAGTENRIAVERMRYNDAVKIYNQAVRTFP
                     ANIVAGMFGFKEAAFFEAPKEAQAPPQVKF"
     gene            complement(3399..4127)
                     /locus_tag="PLR47_05900"
     CDS             complement(3399..4127)
                     /locus_tag="PLR47_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12512.1"
                     /translation="MAKIPDQPQDIFVPLTEDYLEAFGRELVSLMLYGSAAGGSYVKG
                     KSDINLLVVLTPEGMNRLEDAFAPVKKWRKRNVAVPLFLTKEFISSSLDAYPIEFLNM
                     QRHCVLIYGENVLAPLAFKPEDLRLQIEREIKSKIILLREGYLESEGSARRVRELIGR
                     SLVALVSIFNAMLYLKQAGLPRHKKETIEEAGRVFGVDAAVFARCFDIKAKEDHLSGK
                     EVIGVFKQYLREVEKVCHKIDGLK"
     gene            complement(4145..4933)
                     /locus_tag="PLR47_05905"
     CDS             complement(4145..4933)
                     /locus_tag="PLR47_05905"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOG12513.1"
                     /translation="MDEQYDLPARLRIFLALCAVVLIALASGAADAADRFPAHKGTAV
                     NDFAGVIDADNAAKMESLSREILVKTGAAIVAVTLPELGPAEEINLYASGLYQAWGIG
                     KKGEDKGVLILLAVKERKIRIETGYGVEGVLPDGRVGAILDQVVVPHLKTGETGKALY
                     NAMLACGAYLAKDAKVQLTGDFSFYGSSARPEKKGFNIFGILLFLIAAAVLLGTRTGR
                     EMLPWILLMLLSGSGRGGGSGGFGGGFGGFGGGMSGGGGAGRSF"
     gene            5062..6606
                     /locus_tag="PLR47_05910"
     CDS             5062..6606
                     /locus_tag="PLR47_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG12514.1"
                     /translation="MTTAKIIFIHPPVVKPSEPPAGIARLSACLKANGVEHRVIDSNV
                     EGLLYLLRFAVQNGGGTGRWDIRARKNLEANLDLLHTPEAFQNKSRYSRAVMDIHRVL
                     QTAGKPCGVHLSLSDYEDSLRSPVKSADLIQAAEQPEANPFYSYFSGRLTQALEESPD
                     FIGFSMNFLSQALCTMAMIGFVKKIHPRQKIILGGSLTTSWIRLAGRTDLFSGLVDRM
                     VEGAGEEKLPALLGIPCHREGLSADYDLFEKNRYFSPGFILPYSAARGCWWRKCSFCP
                     EKSEAAPYLPLPASRAAEELGTLTKQTGPSLIHLLDSSVSPAFMQALIENPPGSPWYG
                     FARVTRHLADKDFCRALKKSGCVMLKLGLESGSQSVLDDLSKGIDLDEASAALRTLDK
                     AGIAVYGYFLFGTPQETEADAQKTLDFIVRHSGCISFLNLAIFNLPAGSEEARDLSTQ
                     EFYDGDLSLYRNFHHPSGWQRGNVRNFINKTLKKHPAVAPIAWRTPEFFTSNHAPFFA
                     FLKNSK"
BASE COUNT         1441 a         1973 c         1730 g         1406 t
ORIGIN      
        1 cacggcgtgc acggacaaaa cggagacgcc cagacccgcc agaaccgcct ccttgatggc
       61 ctcggagctg cccagttcgc cgcagatgtt gaagtccgca aggcgaacgg cctgttgttc
      121 tttcatatac gattcaaaaa cgtcgcgtgt cgccgaaccc ttttcccgca gtacaaaagg
      181 ctcttccatc agttcggaaa cggaaacgga cccctttttt gtccagcgat gacccgcggg
      241 aaccgccaga atcagccggt catgccagac gggttcggca atcagtttcg cgttttgcgg
      301 gcttttcccg atgcagccga tttccacctc ccggtccagc acctgattga ttacttcttc
      361 actgtcttcc gtcttgagaa aaatccgaat gtcgggatgc ttctttttaa acgagctgac
      421 ggcgcgcggc agaatgtagg tggccggaat cgtgctcgcg ctcagataaa cgtcgccgcc
      481 cggctgtttt cgaagctgat caattttatc cgccgcgtcc cggcgcagac ggacgatccg
      541 ccggacggca tcgtaaagaa ttttgccctc cggcgtcagg gaaacgccgt cgcggctgcg
      601 gttgaccgcc ttcacgccca gacagtcctc cacatggcgg atctgcttgg tcaaagcggg
      661 ctgcgtcagc agcatccgcg cggcggcgcg gctgaaactc cgctcttcca ccaggtagat
      721 cagcgcctcc atctgctgca gggttatatt tttcagccgg tcgtccgtca tatttaactg
      781 cataacataa atatccttta tcctcaaagc ctatgtcgcc cgaaccgggg ttcgcgtcat
      841 tttttcatat tttgtttacg ggccccgacc aattatggta gagtcgcaac ccaaattgga
      901 acgtccccgg aaaacacccg gtcgacccta ttttatgaag gaggaatcta tgaaactgac
      961 agacatgttt tcactcaaag gcaaagtggc gctggtgaca ggcggcggca gaggcatcgg
     1021 caagtttatc gccacgggac tggcggaagc gggcgccgat attattctga cctcgcgcaa
     1081 aataaagaat ctcgaagcaa cagccgccga actggaggcc aaccagaaag tgaaggccgt
     1141 ggcgattgcc tgcgatatgg ccaaggaaga caccgtggaa gccatgctga aggaagccgt
     1201 ggccaaattc ggccgcatcg atattctgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnngc ggggcgtctg ggtgctgacg cagaaagtgg
     1381 cccgcatcat gaaggagcag ggaggcggca acatcatcaa catctcctcc gtcatggcgt
     1441 tccgcggctc catcgaggaa gcccatccgg ccgttcccta caattccacc aaggcggcga
     1501 tcaacctgct gaccatgaac ctcgcagtga agctggcgcc ccacaaaatc cgggtcaacg
     1561 ccattgcgcc gggctttttc cacacggaca tgatggccta tatcgacaag ccggaattca
     1621 aaccggtccg cgacgccatc atcaacgaga ttcccctgcg ccgcgtcggt gaaatcgacg
     1681 acatgaaagg cgtggcggtg tttctggcct ccgacgcatc gcagttcatc accggacatg
     1741 tcatggtcgt ggacggcgga cagatcgcga aatagaaaga aaaggagccg gcgtgcccgc
     1801 atccggacaa cctgccgcgc cgaaagagtc ggcgacggtt attctggtgt gcagccatcc
     1861 ggcggacgcc tggaatgttt ttctggcccg ccgccacagc cgttcgtcct ttatggcgga
     1921 ggcatatgtt tttccaggcg gtcagattgc agacgcagac cgggacgaag atctccggaa
     1981 tttcatttcc atgccgccgg gcttcgatcc gcgggcgcac ctgaaggacg attccctttc
     2041 ccaggaaacg gcgcagagcc tgtttgtctg cgccattcgg gaaacctttg aagaaacggg
     2101 agtcctcatt gcgcggctgc gggacggaag ccccctccag ctgacttcga aagagcaaaa
     2161 cgcccgcctt gccgcctatc gaggggaact gaacgccggc cggaccactc tgagcgagat
     2221 ggcccggcgg gagaatctgg tctttcccct ggacttgctg gttccctacg cccactggat
     2281 tacgccggaa tttctgccga aacgattcag tacccgtttt tttctggccg ctcttccgga
     2341 agaccagtca gccgccaccg atcagggcga actgacggac agcctctggg caaagccgga
     2401 agaagtttta cgcaggtacc gcggcaggga aatcgtcctg atgccgccga ccctgaagac
     2461 gctggaggag ctggcccgat acgcctgcat tgacgacctc tttgccgggg ttcgaagccg
     2521 cgccatttac cccatcctgc ccgagcccgg ggaaaacatg atcaagctgc ctcacgatcc
     2581 ggaatacggc atcgaaagat acaaacaacc gtcccggccc gaagagccgt cgcgcatcat
     2641 catgacggac ggcatctggc ggacgggcgg caaactctga aatggaaccg gacaaagccg
     2701 gtgaagcacg cggcttcacc ggcgctgaat caaagctgaa tcaaaacggc gaaaagcatg
     2761 aggggaacgg attgacgccg ttcccctcat gaataaaggg atcagaattt gacctgaggc
     2821 ggagcctggg cttctttggg cgcttcaaag aaagcggctt ccttaaaccc gaacatgccc
     2881 gcgacgatat tggccggaaa cgtccggacg gcctgattgt agattttcac cgcatcgttg
     2941 tatctcattc tttccacggc aatgcggttt tccgttccgg ccagctcatc ctgcagctgc
     3001 aaaaaattct gattcgactt caaatccggg tagcgctcca ccaccaccat cagacggctg
     3061 agagcgccgg tcaattcgtt attggccgcg atcttgtcgg gaaccgttcc ggctccgccg
     3121 actttcgagc gcgcgttggt cacttccacc agcacgtctt tctcctgctt ggcgtagcct
     3181 ttgaccgttt ccaccagatt gggaatcagg tcgtagcggc gctgcagctg attttccacc
     3241 tgtgcccaag ccgacttgat ggcgacgtcc attcttacaa atcggttgta gttgcccgca
     3301 aaaagggaat acagagaaat cgccaaaacg gcgatgaccg ccaccacaat gatgattttc
     3361 tttcctgccg acattttttt catcctccat cgcttgaatt attttaatcc atcgatctta
     3421 tgacagactt tttccacttc ccgcaaatac tgtttgaaca ccccgatcac ttctttgccg
     3481 gaaagatggt cctcttttgc cttgatgtcg aaacagcgcg caaagacagc cgcgtcgacg
     3541 ccgaaaaccc tgcccgcttc ctcaatcgtt tccttcttgt gccggggaag gcctgcctgc
     3601 tttaaataca gcatggcgtt aaaaatggaa accagggcga ccagcgaccg gccgatcagt
     3661 tccctgacgc ggcgcgccga tccttcggat tccagatacc cctcccgaag caaaatgatc
     3721 ttggatttga tttcccgttc gatctgcagg cggagatcct ccggtttgaa cgccagcggc
     3781 gccagaacat tttccccgta aatcaaaacg caatggcgct gcatgttgag aaattcaatc
     3841 ggataagcgt cgagcgagga agaaatgaat tccttcgtca ggaacagcgg cacggctaca
     3901 ttgcgctttc tccatttttt caccggggca aaggcgtctt ccaggcggtt catgccttcc
     3961 ggcgtcaaaa cgaccagaag attgatatcc gattttccct tcacatagga gccgcccgcc
     4021 gcgctgccgt agagcatcaa cgaaaccaat tccctgccga aggcttcgag atagtcctcg
     4081 gtcaggggaa cgaaaatgtc ctgcggttga tccggtattt ttgccatgtt ctccatcctt
     4141 tatattaaaa actgcggccc gctcctccgc cgccgctcat gcctccgccg aagccgccaa
     4201 acccgccgcc gaagccgccg gagccgccgc cccggccgct gccggaaagc agcatcagga
     4261 gaatccaggg cagcatctcc cttccggttc tcgttcccag aagcacggcc gcggcaatga
     4321 gaaagagcag aattccaaaa atattgaagc ccttcttttc cggacgggcg gagctcccgt
     4381 aaaaggagaa atcgccggtc aactgaacct tcgcgtcttt ggcgagatag gcgccgcagg
     4441 ccagcatcgc attgtaaaga gccttgccgg tctcccccgt cttgaggtgc ggcaccacaa
     4501 cctgatcgag aatcgcgccc acccggccgt ccggcagaac gccctcgacg ccgtagccgg
     4561 tctctatgcg gattttccgc tccttgacgg ccagcagaat cagaactccc ttgtcctcgc
     4621 cttttttccc gatcccccag gcctggtaaa gtccgctggc gtaaagattg atttcttcgg
     4681 cgggccccag ctccggcagc gtgaccgcga cgatcgcggc gcccgtcttt accagaattt
     4741 cccgcgacag ggattccatt tttgcggcat tgtcggcgtc gatcacgccg gcaaaatcat
     4801 tgacggccgt tcccttgtgc gcagggaacc ggtctgccgc atctgccgcg cctgaggcaa
     4861 gcgcaatcag gacaaccgcg cacagagcaa ggaagattcg aagccgcgcc ggcaaatcgt
     4921 attgttcatc cattttcaca aacagctcat caatgttttt ttacgggcgg aactattgcg
     4981 taaagcccgg gccctgtcaa ctcaaatata ggcatggaat attgcttgcg caagcgtgca
     5041 ttcaatacta aacattcgcc gatgacaacc gcgaagatca ttttcattca tcctcccgtc
     5101 gtgaagcctt ccgaaccgcc ggcgggcatc gcaaggcttt ccgcctgcct gaaagccaat
     5161 ggcgtcgagc accgggtcat cgactcgaat gtggaaggcc ttttgtatct gctgcgcttt
     5221 gccgtgcaaa acggcggcgg cacaggccgt tgggacattc gcgcccgtaa aaatctggaa
     5281 gccaacctgg atctgcttca tacccctgaa gctttccaaa acaaaagccg gtattcccgc
     5341 gccgtgatgg acatccaccg cgtcctgcaa acggccggga agccttgcgg cgtccatctg
     5401 tccctgtccg attacgaaga cagcctgcgc tccccggtga aaagcgccga tttgattcag
     5461 gcggccgaac aacccgaagc caatcctttt tactcctact tttccgggcg tctgacgcag
     5521 gcgcttgagg agtctccgga ttttatcggc ttctcgatga atttcttgag ccaggcgctc
     5581 tgcaccatgg ccatgatcgg ctttgtcaaa aaaatccatc cccggcaaaa aatcatcctg
     5641 ggcggatcgc tcacaacctc ctggatccgt ctcgcggggc gaacggatct gttttccggc
     5701 ttggttgacc gaatggtgga aggagcggga gaagaaaagc tgccggcgct gctgggcatc
     5761 ccctgtcacc gggaaggcct ctcggccgat tatgacctgt ttgaaaaaaa ccgctacttt
     5821 tcccccggtt ttatcctgcc ctatagcgcg gcgcggggct gctggtggcg aaaatgttcg
     5881 ttctgcccgg agaaatcaga agccgccccc tacctgcccc tgccggcctc gcgcgccgcg
     5941 gaggaattag gaacgcttac aaaacagacc ggaccgtccc tgattcacct tctggacagc
     6001 tctgtctctc ccgccttcat gcaggcgctg atcgaaaatc cgcccggttc gccctggtac
     6061 ggctttgccc gcgtgacgag gcatctggcg gacaaggatt tttgccgggc gctgaaaaaa
     6121 agcggctgcg tgatgctgaa gctcggcctg gaatccggaa gccagagcgt tctggatgat
     6181 ctctccaaag gcatcgatct tgacgaagcc tcggcagcgc tcaggacgct cgacaaggcc
     6241 ggcattgccg tttacggtta tttcctgttc ggcacgccgc aggaaacgga ggcggacgca
     6301 caaaaaacgc tggattttat cgtccggcat tccggctgta tttctttttt gaacctggcg
     6361 atttttaatc tgccggccgg gagcgaagaa gcacgagact tgtccacgca ggaattttat
     6421 gacggcgatc tgtccctgta ccgcaacttt catcatccca gcggctggca gcgcggaaat
     6481 gtccgcaatt tcattaacaa aaccttgaaa aaacatccgg ctgtcgcccc catcgcttgg
     6541 cgcacgccgg aatttttcac gtctaaccat gcgccttttt ttgcctttct gaaaaattca
     6601 aaataaattt tttcactcta ttgacaatgc ctagatgttg tggttaatgg aagccatcat
     6661 acaatatgta
//
LOCUS       DAOSIM010000108         6602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_10569_length_6602_cov_8.428441, whole genome shotgun sequence.
ACCESSION   DAOSIM010000108 DAOSIM010000000
VERSION     DAOSIM010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6602
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10569_length_6602_cov_8.428441"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PLR47_05915"
     CDS             <1..913
                     /locus_tag="PLR47_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HOG12515.1"
                     /translation="RTQVRYVGSSECSMENGTMRVDANISVRPKGTSQMNTKVEVKNM
                     NSVRHVGDAIAHEIGRQSQAVQTGEAVVLHTRLWDPDKKVTLPMRGKFEGPCVPDPMV
                     PVIELTPEWIEKMRTRLPEMPAARAGRFVKQHGLTGEEAAFLSEDPEVAVYFEALVGR
                     GIAPRTAMHWLATQLIPAVKERKLELAAQPVTPERFAALLKMLAGDEINANAAREVLT
                     HLFESGESPEEIVKTRGFKQVSDTGALEAMIDQVLAAQASAVADFRAGQGKALGFLIG
                     QVMQASGGKANPKIIRELLAKKLNAAS"
     gene            complement(1178..3709)
                     /locus_tag="PLR47_05920"
     CDS             complement(1178..3709)
                     /locus_tag="PLR47_05920"
                     /inference="COORDINATES: protein motif:HMM:NF011291.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOG12516.1"
                     /translation="MQKKYEALSEQVNLTKTLMARSVIYKYLNRTNLICYSELSRLLG
                     CEAFVKHENHNPTGSFKIRGALNFFHHMAWAGQWFHVPCTVVVPENNNPEINRIIESY
                     GAEVIVHGEDFYDAQSYCEELVDSAGYYYVQQGNEPEILNGLATMGLEILEDLPQVDV
                     IICPIGGGAGCASLLKTVRAVNPAIEIIGVEPQNAPSFYASWKKGEVVTLDDAHTVAD
                     GLAARSVFHLPYVILKNTINDVVLLTEDEILEGIKMALTATHNVAEAAGAVSLMAAYK
                     IREKLAGKKVVMIMSGGNLKMDNLRKAGKHEGRVATRFPPEPNGYIHIGHAKSVCLNF
                     GIAFQYSGTCNLRLDDTDPAGESMEYVESIIRDVRWLGFDWGDRLFYASDYFEKLYAF
                     AAELIRTGNAYVCSLNADEVRENRGTFTEPGKESPYRNRSVEENLDLFARMRAGEFPD
                     GAHTLRARIDMASPNIVMRDPVLYRIKREPHYRTGDRWVIYPMYDFAHCLSDALEGIT
                     HSICTLEFENNRPLYDWIVEKLISGGRPRQIEFARLNLSYTVLSKRRLIDLVRNRNVK
                     GWDDPRMPTVTGMRRRGYTPEAIRKFCAEIGVAKNDNLIDVSLLEHCVREDLNDKAPR
                     AMCILRPLKVVIDNYPEKQTEEFDCANHPQHPEMGTRKVPFSREIYIERDDFMEDPPK
                     KYNRLGPGREVRLRNAYIIKYESMVKDEKTGEVTEIHCSCDPATRSAPPADGRKVQGV
                     IHWVSAAHAVPAEVRLYDRLFTIEDPGGEEEFLKYLNSNSLETLSACYAEASLKNARP
                     GAKYQFERLGYFCADPDSAAGRPVFNRVVPLRDSWAKIAGGLKKA"
     gene            complement(3847..5139)
                     /locus_tag="PLR47_05925"
     CDS             complement(3847..5139)
                     /locus_tag="PLR47_05925"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOG12517.1"
                     /translation="MRVVYNILLWIAAVPGLLFFLFKMAFTGKYRHSFLQKLGMRQDY
                     LTTGIGPGPRVWIHAVSVGEVTAAAPVVAALKAKQPEARIIFSTSTETGQGMAQRLVK
                     GADAFIYFPLDISLSVHHMLDVVRPDAFVLVETELWPNFLAACRKRNVSVVMVNGRLS
                     PRSFSKYRRTRFFWRRVLQNVDAAGMISDVDAARIAAMGMDQGKISVLGNAKYDALAA
                     MASPELHEDIARRFHVRENEKFLVAGSTHPGEEEAVVGVYRELLARDPDFRMIIVPRH
                     IERSGDVVRLLREAGFSDVITATQMGSGQRRRDEKVIVVDVIGELFKAYSLATVVYCG
                     GSLVPRGGQNILEAAAWGKVIFYGPSMDDFSSEAALLESAGAGIRIHNAGELMEKILQ
                     TLADPQDAQKRGERGRAVVLANRGAAKRYTEMVARYIG"
     gene            5328..5987
                     /gene="rpe"
                     /locus_tag="PLR47_05930"
     CDS             5328..5987
                     /gene="rpe"
                     /locus_tag="PLR47_05930"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOG12518.1"
                     /translation="MKLIAPSILSADGGRLAEEIAAVEKAGADWIHVDVMDGHFVPNI
                     TMGPAVIKALRKATRLPFDVHLMIQNPDDYIESFADAGADYITVHVEAATHLHRTLGQ
                     IKKAGKKAGVSLNPSTPLSSVEEILPDLDLLLIMSVNPGFGGQKFIQSSLAKIKRAAQ
                     MLAGLPQKPLLEVDGGVNLQNIAAIAGAGADVLVAGAAVFQTPDYAQTIAALKAEANR
                     A"
     gene            complement(6410..>6602)
                     /locus_tag="PLR47_05935"
     CDS             complement(6410..>6602)
                     /locus_tag="PLR47_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOG12519.1"
                     /translation="CNHPIGPLALGDLIGLDTQLRICEGMHRELGDKYRPSPLLRKLV
                     RAGNLGRKSGLGVYDYAKK"
BASE COUNT         1384 a         1940 c         1863 g         1415 t
ORIGIN      
        1 gcgcacgcag gtgcgctatg tcgggtcttc cgaatgcagc atggaaaacg gcacaatgcg
       61 cgtggacgcc aatatttccg tgcgccccaa aggcaccagc cagatgaaca cgaaggtgga
      121 agtcaaaaac atgaattccg tccgccacgt gggcgacgcc atcgcgcacg agatcggccg
      181 tcagagccag gcggtgcaga cgggagaggc ggtggtcctg cacacccgtc tgtgggaccc
      241 ggacaagaaa gtcacgcttc cgatgcgggg caaattcgaa ggcccgtgcg tgccggaccc
      301 gatggtgccg gtcatcgaat tgacgccgga atggattgaa aagatgcgca cgcgccttcc
      361 ggaaatgcct gccgcgcgcg ccggccgctt cgtgaagcag cacggcctca ccggcgagga
      421 agccgctttt ctgagcgaag atccggaggt cgcggtttat ttcgaagcgc tggtcggcag
      481 gggaatcgcg ccgcgcacag ccatgcactg gcttgccacg cagctgattc cggccgtgaa
      541 agaacgcaaa ttggagctgg ccgcgcagcc cgtcacgccg gagcgcttcg cggcgctgct
      601 gaagatgctg gcaggcgatg aaatcaacgc caacgcggcg cgtgaagtcc tgacgcattt
      661 gtttgaaagc ggcgagtcgc cggaggaaat cgtgaagacg cgcggcttca aacaggtttc
      721 cgacacgggc gcgcttgaag ccatgattga tcaagtgctt gcggcgcagg cctcggccgt
      781 ggccgatttc cgcgccggcc agggaaaagc gctgggcttt ctgatcggcc aggtgatgca
      841 ggcttccggc ggcaaagcca atccgaaaat cattcgggag ctgctcgcca aaaagctgaa
      901 cgcggcttca taaaaaagcg gggaacggtc gcgaccgttc cccgcagtca ttagcgggtc
      961 taataacctg aaggtcacaa gcgacccgcc ctgcgtctat tttgtcatcg cgaggagcgt
     1021 aagcgacgtg gcgatctctt tcaatatcaa tagcctatga gattgcttcg tcccgatatg
     1081 tcgggactcg caatgacgga tgtgtattat acaaaccttt tcgcttgaaa gctttgcctt
     1141 gattctattt tcgcgggaat gacgggaaaa aggtccgtca ggcttttttc aagccgccgg
     1201 cgattttggc ccaggaatcc cgcaggggaa caacgcgatt gaaaacgggc ctgcctgcgg
     1261 ccgaatccgg atcagcgcag aaatagccca gccgttcaaa ctggtactta gccccgggac
     1321 gcgcgttctt gaggcttgcc tccgcataac aggccgaaag cgtttccagg gaattcgaat
     1381 tcagatactt cagaaattcc tcttcgccgc ccggatcttc aatcgtaaag aggcggtcgt
     1441 agagacggac ctcggcgggt acggcatgag ccgccgacac ccagtgaatc acgccctgca
     1501 ctttgcgccc gtccgcgggc ggcgcgctgc gggtggccgg atcgcaggag cagtggattt
     1561 ccgtcacctc gccggttttt tcgtccttga ccatgctttc atatttaatg atgtaggcgt
     1621 tgcgcagacg gacctcccgc ccggggccga ggcgattgta ctttttgggg ggatcttcca
     1681 tgaagtcgtc gcgctcgatg taaatctccc gggaaaaagg aactttcctc gttcccattt
     1741 ccggatgctg cggatggttg gcgcaatcga attcttccgt ttgcttttcc ggataattgt
     1801 cgatgacgac tttgagcgga cgcaggatgc acatggcccg gggcgcctta tcgttgaggt
     1861 cttcgcggac gcagtgctcc aagagcgaaa cgtcgatcag gttgtcgttt ttggccacgc
     1921 cgatttcagc gcagaatttc cggatggctt cgggcgtata gcctctgcgc cgcattcccg
     1981 tcacagtggg catgcgcgga tcatcccagc ctttcacatt cctgtttctc accagatcga
     2041 tgagcctgcg cttgctcagc accgtgtagc tcaggttgag cctggcgaat tcaatctgcc
     2101 gcggccggcc gccggaaatc agcttttcga caatccagtc gtagaggggg cggttgtttt
     2161 cgaactccag cgtgcagatg gaatgcgtga tgccctccag ggcgtcggag aggcagtggg
     2221 caaagtcata catcggatag atcacccacc ggtcccccgt ccggtagtga ggttcgcgct
     2281 tgatccggta taaaaccggg tcccgcatga cgatgttggg cgacgccatg tcgattctgg
     2341 ctcggagcgt atgagcgccg tccggaaatt cgccggccct cattcgggcg aacagatcca
     2401 ggttttcttc cacggagcgg tttcggtagg ggctttcctt tccaggctcg gtaaaggtgc
     2461 cgcggttttc ccgcacttcg tcggcgttca aactgcagac gtaggcgttg ccggttcgga
     2521 tgagctccgc ggcaaaagca tagagctttt caaaatagtc ggaggcgtaa aacagacggt
     2581 cgccccagtc aaaccccagc cagcggacat cgcggatgat cgattccaca tattccatgg
     2641 actcgcccgc cgggtccgtg tcgtcgagcc tcaggttgca ggttcccgaa tactgaaacg
     2701 cgatgccgaa attgaggcac accgatttgg cgtgcccgat gtgaatgtag ccgttgggtt
     2761 cgggcggaaa gcgggtcgcc acgcgccctt cgtgtttgcc ggcttttctc aaattgtcca
     2821 ttttcaggtt gccgccggac atgatcatga ccactttttt gcccgccagc ttctcccgga
     2881 ttttgtaggc ggccatcaaa gaaacggcgc ccgcggcttc ggccacgttg tgggtggccg
     2941 tcagcgccat tttgatccct tccagaatct cgtcctcggt cagcagcacc acatcgttga
     3001 tggtgttttt aagaatgaca tagggaagat ggaacacgct gcgggcggca aggccgtcgg
     3061 ccaccgtatg ggcgtcatcc agcgtgacga cctctccctt cttccaggac gcgtaaaagg
     3121 aaggcgcgtt ctgtggctcc acgccgatga tctcgatggc gggattgacg gcgcggacgg
     3181 ttttcagcag cgacgcgcac cccgcgcctc cgccgatggg gcaaatgatc acatcgacct
     3241 gcggcaggtc ctccagaatc tccagcccca tggtggccag gccgtttaga atttccggct
     3301 cgttgccctg ctgaacatag taatatccgg cgctgtccac cagctcctcg cagtaggact
     3361 gcgcgtcgta gaagtcttct ccgtggacaa tcacttccgc gccatagctt tcgatgatgc
     3421 ggttgatttc cgggttgttg ttttccggca cgaccaccgt gcagggcacg tggaaccact
     3481 ggcccgccca ggccatgtga tggaaaaagt tcagggcgcc ccgaatcttg aaagacccgg
     3541 tcggattatg gttttcatgc ttgacgaagg cctcgcagcc gagaagcctg gagagctccg
     3601 aatagcagat cagattcgtc cggttgaggt acttgtagat caccgacctc gccatcaggg
     3661 tttttgtcag attaacctgt tcgcttagcg cttcgtattt tttctgcata cacaacctcc
     3721 ccggccgtag cagccgatct cttttaaacc actttttcgc ccggcgtcaa gtgaacttca
     3781 cttttattcc cttcagcctt cagccttcag ccttcggtct cgtgtgcccc aaaggggtat
     3841 cagtcttcag cctatatacc tggcaaccat ctccgtatag cgttttgcgg cgccccggtt
     3901 ggccaggaca accgcccgtc cccgctcacc ccgcttttgt gcgtcctgcg gatcggcaag
     3961 cgtttgcaga atcttctcca tcaactctcc ggcgttgtga atgcgaatgc cggcgcccgc
     4021 cgattccaga agcgcggctt ccgagctgaa gtcgtccatg gacgggccgt aaaaaatcac
     4081 tttcccccag gcggcagctt ccaggatgtt ctggccgcct ctcggcacga gcgagccgcc
     4141 gcagtaaacc accgtcgcca gcgaatacgc cttgaaaagc tctcctatca catcgacgac
     4201 gatcactttt tcatcccggc gccgttgccc gctccccatc tgtgtcgcgg tgatgacgtc
     4261 agaaaagccc gcctcccgca gaagccgcac cacgtcgccg gagcgttcaa tgtgccgcgg
     4321 cacaatgatc atgcgaaaat ccggatcacg cgccagaagc tcccgatata ccccgaccac
     4381 agcctcctct tcaccgggat gcgtgcttcc cgctaccagg aatttttcgt tttcgcgcac
     4441 atgaaaccgg cgggcaatat cctcatgcag ctcaggcgat gccattgccg caagcgcgtc
     4501 gtacttggca ttgcccagaa cactgatctt tccctggtcc atgcccatgg cggcaatccg
     4561 ggcggcgtcc acatcggaaa tcatcccggc ggcgtccaca ttttgcagga cccgacgcca
     4621 gaaaaaacga gtccggcggt atttggaaaa agagcgggga gaaagacggc cgttcaccat
     4681 cacaacggaa acatttcgct tccggcaggc ggccagaaaa ttgggccaga gctccgtctc
     4741 caccaggaca aacgcgtccg gacggacaac atccagcatg tgatgaacgc tcagggaaat
     4801 atccagcgga aaatagataa aagcgtccgc gcccttcacc agtctttgcg ccatgccctg
     4861 ccctgtttcc gtcgatgtgg aaaatatgat tcgcgcttcc ggttgtttcg ctttcagggc
     4921 ggcgacaacc ggcgcggccg ccgtcacctc ccccacggaa accgcgtgaa tccaaacacg
     4981 gggacccggt ccgatacccg tcgtcaaata atcctgacgc atgcccagtt tctggagaaa
     5041 actatggcgg tacttgcccg tgaatgccat tttgaaaaga aaaaaaagca gtcccggaac
     5101 cgccgcaatc cacagaagaa tgttatagac caccctcatg cgcgcatctt tgattttttc
     5161 agctttggtg tcaaggggaa aggagtggat agtgaatggt tgatagttga actttaacct
     5221 ttcgccatcc actatccact atccgctatc caccattcac catcgcggcg gttgacagga
     5281 ataatgcttt agtatattga atttaccgac aaaaggagac ctcttcgatg aaactgattg
     5341 ctccttcgat tctttccgcc gacggcggaa ggctggccga agaaatcgcc gcggtggaaa
     5401 aagccggcgc ggactggatc cacgtggacg tcatggacgg gcattttgtc cccaacatca
     5461 cgatggggcc ggccgtcatc aaggctttgc gaaaagccac ccggctgcct tttgacgtgc
     5521 acctgatgat tcaaaatccg gatgactaca tcgaatcctt cgcggacgca ggcgcggatt
     5581 atattaccgt ccacgtggag gcggccacgc acctgcaccg tacgctcggg cagatcaaaa
     5641 aagccgggaa aaaagccggc gtgtcactga atccgtccac gccgctttct tccgtggagg
     5701 aaatcctccc cgacctcgac ctgcttttga tcatgtcggt caatcccgga ttcggcggac
     5761 agaagttcat tcaaagctcg cttgccaaaa tcaaacgggc cgcccaaatg cttgccggtc
     5821 tgccgcaaaa gcctctgctg gaagtggacg gaggggtgaa tctgcaaaat atcgcagcca
     5881 ttgcgggcgc gggcgcggat gtcctggtcg cgggcgcggc ggttttccaa acgcccgact
     5941 acgcacaaac catcgccgcc ctcaaagccg aggccaaccg ggcctgatct tttttacaga
     6001 cgacccttaa caggctgtcg agatatccct ttgagcggcc attgcttgtg acctttaggt
     6061 taagagggtt ttgtccatat aattgaaggc ctgcggcctt cccggaaagg aataaatttc
     6121 aattatttgt tgcgctccgc agatgctgcg cgctcccggt cttggcgaag cgtaagatca
     6181 gcaatgacaa ctgtttgagc cgaaggcgag ttttgtcaaa gccttccgcg agccttagat
     6241 ttatacaaaa cactctcagg caagtgaaaa gggtatttgg gcagcagcat ggcagggatt
     6301 gacggatttt ttcctaagcg cggtcatagc agggacgggc caagacccgt ccctgctttt
     6361 tttatccttc accatgagcc gtcatgaccg gacagcccgg cacattccgt tattttttgg
     6421 catagtcata cacgccgagg ccggatttgc gccccaggtt gccggcgcgg accagcttgc
     6481 gcaaaagcgg cgacgggcgg tatttgtcgc ccagttcgcg gtgcatgccc tcgcagattc
     6541 gaagctgcgt gtccagaccg atcaggtcgc ccagcgccag cgggccgatg ggatgattgc
     6601 ag
//
LOCUS       DAOSIM010000109         6553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_10697_length_6553_cov_7.532625, whole genome shotgun sequence.
ACCESSION   DAOSIM010000109 DAOSIM010000000
VERSION     DAOSIM010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10697_length_6553_cov_7.532625"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..444)
                     /locus_tag="PLR47_05940"
     CDS             complement(187..444)
                     /locus_tag="PLR47_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12520.1"
                     /translation="MNIYEVLDYHTDETYPNLMDAIHHFSDGLECYQKGQWDRAMEAF
                     RRGLSQNGGDQLSRMYIDRCEYMKNHPPSGEWNGVFVMTSK"
     gene            complement(462..614)
                     /locus_tag="PLR47_05945"
     CDS             complement(462..614)
                     /locus_tag="PLR47_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12521.1"
                     /translation="MKNLRNQSTVKRLNARKLQTLPDVPRKVAALETLNDILDELMKI
                     TTTGWW"
     gene            complement(616..885)
                     /locus_tag="PLR47_05950"
     CDS             complement(616..885)
                     /locus_tag="PLR47_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12522.1"
                     /translation="MRDQLENTCYEKDCAAQEDMAAAGNREKQLTNTIAALRNELERR
                     DIDRQRDMQTMARSHGNETAQLRRTIQALRDRLETFKNSRTAKAG"
     gene            1073..1222
                     /locus_tag="PLR47_05955"
     CDS             1073..1222
                     /locus_tag="PLR47_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12523.1"
                     /translation="MQGHIIATGITFGTALAITISYSYSHSVLWAIFHGILSWVYVIY
                     HVLFH"
     gene            1395..3026
                     /locus_tag="PLR47_05960"
     CDS             1395..3026
                     /locus_tag="PLR47_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOG12524.1"
                     /translation="MPITEILTRNASLYCEETSLVEINPQIKEVKASWKDYELVETNP
                     ENRYRREMTWREFDDKANRLANFLLQRGLEKGRKVGILLMNCLEWLPIYFGILKTGAI
                     AVPLNYRYTEEEIKYCLDLADCDVLIFGPEFTQRIEAIREQMPKVKLRLFVGQDLPAF
                     AESYYALTACCSSVAPRVPLSDADDAAIYFSSGTTGFPKAILHHHRSLMMACEVEMNH
                     HRQTREDNFLCIPPLYHTGAKMHWFGSFLAGSRAVLLRGVKPEWVFRAVSEEKITIVW
                     LLVPWAQDILDAVERGDLKLKDYQLDQWRLMHIGAQPVPPSLVRRWREHFPTHDYDTN
                     YGLSESIGPGCVHLGLENIHKVGAIGKPGYQWEIRIVDENGAPVKQGDVGELCVKGDG
                     VMKGYYKNPEATAEILKDGWLFTGDMARMDEDGFVYLVDRKKDVIITGGENIYPVQIE
                     DFLRTHEAIKDAAVIGLPDHRLGEIVAAVIELKPGFAGSEAEMAKFCEALPRYKRPRK
                     IFFEQVPRNPTGKIEKPKLREKYYAGSLVEAETEG"
     gene            complement(3069..3767)
                     /locus_tag="PLR47_05965"
     CDS             complement(3069..3767)
                     /locus_tag="PLR47_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOG12525.1"
                     /translation="MTGRSGKVSGGRGDGIEEHQDRPMIKALFWDNDGILVDTEGIFF
                     RANQETLADVGISLQWEQFEEISLVRGGSVLQLAEKPQTEAFYKLRDRRDALYADLLT
                     REPLVMEGVREVLEALKGKYVMGIVTSSGQEHFEIIHSRSGLLPYFQFALTMKDCAHF
                     KPHPEPYLKAIALSGCQPEECLAVEDSPRGLASAVNAGIRCMVVPSRFTRGGDFTGAF
                     RILNNMRELPQALD"
     gene            3933..4832
                     /locus_tag="PLR47_05970"
     CDS             3933..4832
                     /locus_tag="PLR47_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG12526.1"
                     /translation="MKDRQFGRVRFIRGENGGKYPFSHSLYLQGDRERVVIDPASSLE
                     KLTRLKEEGVDGVWLSHWHEDHIRYMNLFEGCPVWVSERDFPPFTDLETFLDWYGIED
                     RGARYYWKKEMLETFDYRPRQTACFLQDDKRMDLGGLTVQVIPTPGHTPGHLSFFFPE
                     EELLFLGDYDLTSFGPWYGDILSDIAHTIASIHKLKAIPARRWVAAHNHGIYESNPGP
                     LWDQYEKIIYERERKLLAFLDRPKTPREICSAWLIYGRPREPKEMYEFNERVLIGKHL
                     DYLRQRGKAVLEDGRWRKVESEI"
     gene            4895..5350
                     /locus_tag="PLR47_05975"
     CDS             4895..5350
                     /locus_tag="PLR47_05975"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOG12527.1"
                     /translation="MIAYQLCVFAEDKPGQLANVTSVLAKADISIRATTISTAGTSGV
                     INLIVDDPKSAAKALANAGLTVHLKNVLAVLIPDRPGGLDKLMQLLYRENININNACG
                     FVLECAEKAVFVVDVDQIEKTEKLVEKNGFKTLDTEALSAVEPFHYMKY"
     gene            5516..5950
                     /locus_tag="PLR47_05980"
     CDS             5516..5950
                     /locus_tag="PLR47_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12528.1"
                     /translation="MAHQDKGKYFKKHPEGTTVSDKLKEAVQKSAKDNNVSCKAAEKI
                     AQSTGFSLGEVGVAIDMLNINIAQCQLGLFGLDGKQKKVLPAPSVAPDLDAAIRKALV
                     NGRLSCAAAWEIAARLGIKRMEVCAACENLKIKVKPCQLGAF"
     gene            6302..>6553
                     /locus_tag="PLR47_05985"
     CDS             6302..>6553
                     /locus_tag="PLR47_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12529.1"
                     /translation="MKEAVSNMILNWQDTYKERLTDARSALSQIRRGARIFIASACGE
                     PQLLVKTLLDMAQNFADVEVIHFLDLGLTDYTSEIYTEHF"
BASE COUNT         1493 a         1760 c         1797 g         1503 t
ORIGIN      
        1 cggccagggc ggcgccgttt acatgatgaa tgtgcagccg caaatcgaga aggtttttga
       61 gattgccaac ctgctgaagg gaatgaagct gtttgccggc acgaaggaag ccgacgagta
      121 tttcgacgcc attcagaaaa gcgttttgga atcctcgacg taaggcctga gacttttttt
      181 gcgcgcttac tttgacgtca tgacgaagac gccgttccac tcgccggacg gcgggtggtt
      241 tttcatgtat tcgcagcggt cgatatacat ccgggaaagc tgatcgccgc cgttttgaga
      301 caatccgcgc cggaacgcct ccatcgccct gtcccactgc cccttctgat agcattccag
      361 gccgtccgag aaatgatgga tggcgtccat aaggttgggg tacgtttcgt cggtatggta
      421 atcgagcact tcgtagatgt tcaccggctc tgtttttccc ttcaccacca cccggtcgtg
      481 gtgattttca tcagttcgtc caggatgtca tttaaggttt ccagcgccgc cacttttcgc
      541 ggaacatccg gcagcgtctg cagcttcctg gcgttcaggc gttttaccgt tgattgattc
      601 cgaaggtttt tcattttatc cggcctttgc ggttctcgaa tttttgaagg tctccagacg
      661 gtccctcaac gcctggatgg tccggcgcaa ttgcgccgtt tcatttccat gcgatctggc
      721 catcgtttgc atgtcccgct gacgatcgat gtcccggcgc tccagttcat tgcgcagggc
      781 tgctatcgtg ttggttaact gcttttccct gttccctgcg gcggccatat cctcctgcgc
      841 cgcgcaatct ttttcatagc aggtgttttc cagctgatcc cgcatggcgg aaacggcggc
      901 gcgctttgta tttgacgttg gcgcgcttta taaagtcaat tccggtgcga tttcaaccga
      961 aaataatttg agggagaacg cctgttcccc ctgttttctt gacagggaag gcgggaattt
     1021 ctataatgcc gcccgagaac atcgaaatcc tacgcaatca caggaggttg tcatgcaggg
     1081 tcatatcatt gccaccggaa ttacgttcgg cacggcgctg gccatcacca tcagctacag
     1141 ctacagtcat tcggtgctct gggccatctt tcacggcatt ctctcgtggg tttacgtgat
     1201 ttaccatgtc cttttccact gaggatgcgg cgaagccgtt tcccggccgg caagatattc
     1261 ttgacatcaa cgcctgaaat tcgtatcgtg cgttccgaaa aaaacgtgtt gttgtgccgt
     1321 gtgcagctga tatgtcaaaa tattctggaa taccgccgca aatccatcaa tcccatgagc
     1381 agaaggaatc ctaaatgccc attacagaaa ttctaacccg aaacgcttct ctctactgcg
     1441 aagaaacaag cctggtggag atcaatcctc aaatcaaaga agtaaaggca agctggaagg
     1501 attatgagct ggtcgaaacc aatccggaaa atcgctaccg tcgggagatg acctggcgcg
     1561 agtttgacga caaggccaac cgcctggcca actttctttt gcaaagaggt ctggaaaagg
     1621 ggcgcaaggt tggaatattg ctgatgaact gtctggaatg gctgccgatc tatttcggca
     1681 tcctcaagac gggcgccatt gccgtcccgc tcaactaccg ctatacggaa gaagaaatca
     1741 aatactgtct ggatctggcc gattgcgacg ttttgatctt cgggccggaa ttcacccagc
     1801 gaattgaagc gatccgcgaa cagatgccga aagtaaaact ccgcctgttt gtgggacagg
     1861 acctgcccgc gttcgccgaa agctactacg cgctcaccgc gtgctgctcc tccgtggcgc
     1921 cgcgcgttcc gctttccgac gcggacgacg cggccatcta tttttcatcc ggcactaccg
     1981 gctttcccaa ggcgatcctt catcaccacc gcagcctgat gatggcctgt gaagtggaaa
     2041 tgaaccatca ccggcagacg cgcgaagata atttcctgtg cattccgccg ctctaccaca
     2101 ccggcgcgaa gatgcactgg ttcggaagct tcctggccgg aagccgcgcg gttttgctgc
     2161 gcggcgtcaa accggaatgg gttttcaggg cggtgtccga agagaagatt acgatcgtct
     2221 ggcttctggt gccgtgggcg caggatattc tggacgccgt cgagcggggc gacctcaagc
     2281 tgaaagacta tcagctcgac cagtggcggc tgatgcacat cggcgcgcag ccggttccgc
     2341 cgagcctggt ccggcgctgg agggaacatt ttccaaccca cgattacgat accaattacg
     2401 gcctgtcgga atccatcggt ccgggctgcg ttcacctggg gctggaaaat atccacaaag
     2461 tcggcgccat cggaaagccc ggctatcagt gggaaatccg gattgtggat gaaaacggtg
     2521 ctcccgtgaa gcagggcgac gtgggcgagc tttgcgtcaa gggtgacggc gtgatgaaag
     2581 gctattacaa aaaccccgaa gccacggccg agatcctgaa ggacggctgg ctgttcacgg
     2641 gcgatatggc gcggatggac gaagacggct ttgtctatct ggtggaccgc aagaaggacg
     2701 tcatcatcac gggcggcgaa aacatctatc cggtgcagat cgaggacttt ctgcgcacgc
     2761 acgaagccat caaggatgcg gcggtgatcg gactgcccga ccatcggctg ggggagatcg
     2821 tcgcggcggt catcgagctt aagccgggtt ttgccggctc ggaagccgaa atggcaaaat
     2881 tctgcgaggc gctcccgcgc tacaagcgtc cgcgcaaaat cttttttgaa caggtgccgc
     2941 gcaatcccac cggaaaaatt gaaaagccga agctccgcga gaaatactac gccgggagcc
     3001 ttgtcgaagc ggagacggag ggttgatgga cgactccgca aaacaatgat cggttgattc
     3061 gggacagatc aatccagcgc ttggggaagc tccctcatgt tgttcaggat tctgaaggct
     3121 cccgtgaaat cgccgccccg tgtgaatcgg ctcggaacca ccatgcaccg aatccctgcg
     3181 tttacagcgg aggcaagacc gcgcggcgaa tcctcgacgg caaggcattc ctccggctgg
     3241 cagccggaga gcgcgatcgc tttgaggtac ggctcgggat gcggtttgaa atgcgcgcag
     3301 tctttcatgg tcagagcaaa ttgaaaataa ggcagaagcc ccgaccggct gtgaataatc
     3361 tcgaagtgct cctgtccgga gctcgttacg attcccatca cgtattttcc cttcaacgcc
     3421 tccagcactt cccgaacgcc ttccatgacc agcggctccc gcgtcagcag gtcggcgtaa
     3481 agagcgtccc gcctgtcgcg cagtttataa aacgcctccg tttgcggctt ttccgccagt
     3541 tgcagcacac tcccgccccg gacaagcgag atttcttcaa actgctccca ttgaagcgaa
     3601 atgcccacat ccgccagcgt ctcctgattg gccctgaaaa aaatgccttc cgtatcgacc
     3661 aaaatgccgt cattgtccca gaaaagtgcc ttgatcatcg gcctgtcctg atgctcctca
     3721 atcccgtctc cccggccgcc gctcactttt ccgctgcggc ctgtcattat ccgatgcgtt
     3781 gcagtggaaa accaacgatc tcaaatgatc ctgcaagata cagtaactgg aaaaaccatt
     3841 tgtttcaacg agttttcgct tatggaattc ttgatggcaa tttggccgga tattttctat
     3901 attccaccac caccataccg ggagtttcag tcatgaaaga tcgtcaattc ggcagggttc
     3961 gatttattcg cggtgaaaac ggcgggaagt atccctttag ccattccctg tatctgcagg
     4021 gcgatcggga gcgagtggtc atcgatcccg cctcttcgct ggaaaagttg acgcggttga
     4081 aggaggaagg cgttgatggc gtctggcttt cgcactggca tgaagatcac attcgctaca
     4141 tgaacctctt tgaaggttgt ccggtttggg tctccgaacg cgattttccg ccgtttaccg
     4201 atctggaaac atttctggac tggtacggga ttgaagacag gggcgcccga tactattgga
     4261 agaaggaaat gctggaaacg tttgattacc gcccgcgtca aacggcgtgt tttcttcagg
     4321 atgacaagag gatggatctg ggcgggctca ccgtgcaggt cattcccacg cccggccaca
     4381 cgcccggcca cctgtctttc ttttttcccg aggaagagct gctttttctg ggggattacg
     4441 atctgacgtc gttcggtccg tggtatggcg atattttatc cgacatcgca cataccatcg
     4501 cctcgattca caagctcaaa gcgataccgg ccaggcgatg ggtggccgcg cataatcacg
     4561 gaatttatga atccaatccc ggcccgctct gggatcagta tgaaaagatc atttatgagc
     4621 gggaaagaaa gctgctggcc ttcctggacc ggccgaaaac gccccgtgag atctgctcgg
     4681 cctggctgat ttacggcagg ccccgcgagc cgaaggagat gtacgaattc aacgaaaggg
     4741 ttctgatcgg aaagcacctg gattatttgc ggcagagggg taaagcggtt ctggaagacg
     4801 gccgctggcg gaaggtcgaa tccgagattt gacttgctaa aacttggtca tcattgtaaa
     4861 aagactcaac ggcaaagaaa atcaggagaa gaacatgatt gcgtatcagc tttgtgtttt
     4921 cgcggaagat aagccggggc aattggccaa tgtcacaagc gttctggcaa aggccgatat
     4981 cagcattcgg gcgacaacca tctcgaccgc cggcacatcc ggggtgatca acctgattgt
     5041 ggatgatccg aaaagcgctg caaaggcgct cgccaacgcg ggattgacgg ttcacctgaa
     5101 aaacgttctg gccgtgctga ttcccgaccg gccggggggg ctggataagt tgatgcagct
     5161 tttgtacagg gaaaacatca acatcaacaa cgcctgcggc tttgttctgg aatgcgcgga
     5221 aaaagccgtg tttgtggtgg acgtggacca gattgaaaag acggaaaaac ttgtggaaaa
     5281 gaacggattc aagacactgg ataccgaggc gctttcggcg gtggagccgt ttcattacat
     5341 gaaatattga cgatcttccg cattcatcgg ctaactttgt tggcgttgtc gtcggcatcc
     5401 tcgacgtatt cataatacgc ctgcggtgcc tcctcctagc cgccgcgtta tccttcgaat
     5461 gcaaaagatc gtgatatttt caacctcgat ttctcagagg aaaggagaga aaaccgtggc
     5521 acatcaggac aaaggcaaat acttcaagaa gcatcccgaa gggacaaccg tcagcgacaa
     5581 gctgaaagag gcggttcaga agtccgcaaa agacaacaat gtttcctgca aggcggcgga
     5641 aaaaattgcc cagagcacag gtttttcgct tggcgaagtc ggcgtggcca ttgacatgct
     5701 caatattaat attgcgcagt gccagctcgg cctcttcggg ctggacggca agcaaaagaa
     5761 agtgctgccc gcgccatccg tcgcaccgga cctggatgcc gccatccgca aagcgctggt
     5821 caacggccgg ttgtcctgcg ccgcggcctg ggagatcgcg gcacggctgg gcatcaagcg
     5881 tatggaggtc tgcgccgcct gtgaaaacct gaaaatcaag gtcaagccct gtcagctcgg
     5941 agctttttga tgggagtttt ttcaccctca ccctcaccct aaccctctcc cctcaaggga
     6001 gagggaagta aaaggatgcc ccacttcctc ttttatctcc attacgtcgg cgagtgggcc
     6061 aaaggcgaag aagaagtaaa aggatgcccc acctcctctt ttatctcctc ttctctctaa
     6121 gggagaggga agtaaatgtg aagtggctcc acagtttacc ttctgcgtct ttttatctcc
     6181 cctccccttg tgggaggggt ggggggaggg ggtcatggtt cgacgagctc accatgacaa
     6241 tatttgtaaa ttatgacttt ttacaggttg atcaagagta gtttttcaaa gcactcgatg
     6301 catgaaagaa gcggtgagca acatgattct caactggcag gatacctaca aggaaagact
     6361 caccgatgct aggagcgctt tgtcccaaat ccggcgcgga gcgcgcatct tcattgcttc
     6421 cgcctgcggc gagccgcagc ttctggtcaa aacccttctg gatatggcgc agaatttcgc
     6481 cgacgtggag gtgatccatt ttctggatct ggggctcacc gactacacgt cggaaatcta
     6541 cacggagcat ttc
//
LOCUS       DAOSIM010000110         6433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_10906_length_6433_cov_8.014268, whole genome shotgun sequence.
ACCESSION   DAOSIM010000110 DAOSIM010000000
VERSION     DAOSIM010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6433
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10906_length_6433_cov_8.014268"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(471..3851)
                     /locus_tag="PLR47_05990"
     CDS             complement(471..3851)
                     /locus_tag="PLR47_05990"
                     /inference="COORDINATES: protein motif:HMM:NF016358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Swt1 family HEPN domain-containing protein"
                     /protein_id="HOG12530.1"
                     /translation="MAITNHERVGKALSLLKEGLRPFVERELKSQYQQSWFDELKASL
                     PPQQLSFAGTEAEPFGDIAVVLAALWNQWNSVFKKTLGHTERSLVSELRDHRNKWAHQ
                     EPFSGDDADRALDSAERLLAAVSAPQAEDVRKLKMELRRTIYDEQVRSEKRKSAGSAI
                     ESAAAGNLKPWREVVTPHKDVASGRYQQAEFAADLWQVRLGEGTPEYRDPIEFFRRTY
                     LTESLKRMLVGAVRRLSGQSADPVVQLQTNFGGGKTHAMLALYHLLSGIAPTELAGID
                     AVMQEAGTATLPSVRRVVLVGNRISPGNPVRKQDGTVVRTLWGELAWQLGYAAGGESE
                     AGKAFDQLAADDERATSPGDVLRELFVRYGPCLILIDEWVAYARQLHDQSDLPGGSFE
                     TQFTFAQALTESAKLAGNCLLVISLPASDTSGSPHAQTDDAEVGGQRGREALDRLRNV
                     VGRLESSWRPASAEEGFEIVRRRLFEPLADPAQFKDRDVVARAFADFYRTQQAEFPPE
                     CRDADYEKRIRAAYPIHPEIFDRLYTDWSTLVKFQRTRGVLRLMASVIHSLWEKGDRN
                     PLIMPANISIDDSRVQSELTRYLSDNWPPIIERDVDGPNSLPLKIDGEVPNLGKFAAC
                     RRVARAIYLGSAPLTKAAHRGLEDRQVKLGCVMPGESPNVFGDALRRLAAAATYLYQD
                     GPRYWYSTQPTVTKLAEDRAEQLKRDPDKVMQELDGRLRKDLERKGDFSRIHPLPQSG
                     QDVSDDLDARLVVLGINHPYSKEPGSAAEAAAKAILESRGSSPRHYRNTLVFLAADKT
                     RLQDLDEAARKFLAWESILTEKGEKGLNLDPQQVKQAETQRTAADSAVTARLPETYQW
                     LLVPVQSTPQSAVEWQALRLSGQDALAVRASKKLKNDELLITTFAATRLRMELDRIPL
                     WRGNHVAIKQLAEDFASFSYLPRLKDSSVLVGAIRTGLPLMLWMQDSFAFADSYDESA
                     NRYRGLRCGVEVYIAGGETQGLLVKPDVACRQMDEEKAKTTVGGVTVGPTAGGEGTTT
                     IIPVGGPMGTPDTPAVAPKLKRFHGTVILDAARVGRDASRIADEVIAHLSGLVGATVT
                     VTLEVEADIPSGAPDHVVRTVTENSRTLKFASQEFEKE"
     gene            complement(3856..4446)
                     /locus_tag="PLR47_05995"
     CDS             complement(3856..4446)
                     /locus_tag="PLR47_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HOG12531.1"
                     /translation="MKKIVFLLEEPSMKAFIEEFLPRLIPDLEFLCITHEGKQDLEKS
                     IPRKLKAFRRELFVIIRDNDGADCRAIKARLIDVCKNEGKSDVLIRIACQELESWYLG
                     AMDILAAVYARPKLTGLNLKSKYRNPDRLGNPSSEVIKLIPEFRKIEGARRMGMAMPL
                     EKSDNYSRSFQVFIEGIQRMLKSTTGKKVRLRIRRE"
     gene            complement(4449..5618)
                     /locus_tag="PLR47_06000"
     CDS             complement(4449..5618)
                     /locus_tag="PLR47_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG12532.1"
                     /translation="MQIESIDILNYRLFKEASLMHLKRLAVLVGENGAGKSTFFDVFS
                     FLKDALAQNVAKAVAKRGGFKELVSRGTEGPISIVLKFRETGKRLATYELSVAFEGGK
                     VVVDREVLKFRRGQRGQPWHFVDFSRGKGTAITNEAVYGEPGAKKERKEYILDEPTVL
                     AIKGLGQFKEFRVVSEFRNLIENWHITDFHIADARPSAEEGYAEHLSTRGDNVAQVAQ
                     FLFDHHQDRFNDILDAMKRRIPGVEKVEAKPTEDGRLVLRFQDGAFKDPFIARYVSDG
                     TIKMFAYLVLLHDPNPFPLLAVEEPENQLYPELLQELAEEFRDYARRGGQVFISTHSP
                     EFMNGLNLDEIYWLTKKEGYATIHRGADSEILKSLVAEGDYPGTLWRQGVFKGAW"
     gene            complement(5674..>6433)
                     /locus_tag="PLR47_06005"
     CDS             complement(5674..>6433)
                     /locus_tag="PLR47_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12533.1"
                     /translation="RKRVADAPISTRREFINVLKAELPVALAHLQRSNIAPVDLAQAA
                     IGPGMAIFTRYAKVLDAEGKPLTVREALALINQTLDEVLAEQEGDFDADSRWALAWFE
                     QAGFDEGEYGVAEVLSKAKATSIDGMKRDGILASTGGRVRLLRPDELSAAWDPEKDKR
                     IPAWEMVHQLVRALETGGEGAAAVLVTKLGSKAEVARELAYRLYTLCERKKRAAEALS
                     YNSLVQSWPEIMRLATGSEKSRGVQADIFDNDEI"
BASE COUNT         1329 a         1916 c         1683 g         1505 t
ORIGIN      
        1 gggcctgccg gtttgcacat gcggaggtgg tgcgtttgga taatgtgcta tgccgtcttt
       61 tcgggtggtc atgcgctgcc gggcatggtt cagcggcctg tgggccaacg cctcgaacct
      121 atcatgttgt aaaacctaaa ctacaaatta tctaatggaa aatctggatt taatgaacat
      181 caaacttcgc tttcaccccc cgttgtggcc ctgcgggaat tttgacgcgg cctttgtcgg
      241 agggcaaaac tttttccgcg tcgtctaaaa ctcaataata tcaagtagat tatatatgtc
      301 ggagctccga cataccccct gaaaaagtcc cttcaccgcc ggtgtaaaag gacaaatatc
      361 cgagatgctg aggatatatt ctcgtatgcc gtcatgaccg tttcacctta attaattgat
      421 aagacgcctg tccaaaatac ctggagccaa aatactgcgc cagacccctt ctattccttc
      481 tcaaactcct ggctcgcgaa tttcagtgtc cggctgtttt cggtgacggt gcggaccacg
      541 tgatccggag caccggaggg aatatccgcc tcgacctcca gtgtgacagt tacagtggca
      601 ccaaccagac cggagagatg ggcaatcacc tcatcggcaa tacggctggc gtcgcgtccg
      661 acgcgcgccg catccagaat taccgtcccg tggaagcgct tcagtttcgg cgctaccgca
      721 ggtgtgtctg gcgttcccat gggaccacct acaggtataa tagttgtcgt cccttctcca
      781 cccgcagttg gtccgacagt cacaccaccc acagtcgttt tcgctttctc ttcgtccatc
      841 tgcctacagg ccacatccgg tttcaccagc aacccctgcg tctctccccc ggcaatgtaa
      901 acctccacac cgcagcgaag ccccctgtat cggttggcgc tttcatcata gctgtcggca
      961 aaggcaaagg agtcctgcat ccacagcatc aagggcaacc cggtgcggat ggcgccgact
     1021 aagaccgatg aatccttgag tctgggcaga tagctgaagc tcgcaaagtc ctcggcaagc
     1081 tgcttgatgg caacatggtt gccacgccag aggggaatcc tgtccagctc cattcgcagc
     1141 cgtgttgctg caaaggtcgt aatcagcaat tcatcgttct tgagcttctt acttgcccgt
     1201 acggccagtg catcctgccc ggaaaggcgc agcgcttgcc attcgacggc cgattgcggc
     1261 gtcgattgca ccggcaccag cagccactga tacgtctccg gcaggcgggc cgtcacggcg
     1321 ctgtcggccg cagttctctg tgtttcggct tgctttacct gttgcggatc gaggtttagt
     1381 cctttctccc ctttttcagt caggattgat tcccaggcga gaaatttccg ggccgcctcg
     1441 tcaaggtcct ggagacgtgt cttgtcggcg gcaagaaaaa cgagggtgtt gcgatagtgg
     1501 cgcggggagc tgccccgcga ttccagaatc gcctttgcgg ctgcctctgc ggcgcttccc
     1561 ggctccttgc tgtagggatg attgatcccc aagacaacca gacgtgcgtc aaggtcgtcg
     1621 gaaacatctt gccccgattg tggcagggga tggatacggc taaagtcacc cttgcgttcc
     1681 agatccttgc gcagccgtcc gtccagttcc tgcatcacct tgtcgggatc gcgcttgagt
     1741 tgttcagcgc ggtcctcagc caacttggtg accgtgggct gggtcgaata ccagtatcgc
     1801 ggaccatcct ggtagagata ggtggcagcg gcagccaatc tccgcagggc gtctccgaaa
     1861 acgtttggcg attctcccgg catgacgcat ccgagcttca cctgccggtc ctcaaggccg
     1921 cgatgggcgg ctttggtcag gggcgctgac cccagataga tggcacgggc cacacgtcgg
     1981 caggccgcaa atttgcccag gttgggcact tcaccatcga ttttaagcgg cagcgagttc
     2041 ggaccatcca cgtctctttc gatgatcggc ggccagttgt ccgaaagata gcgggtcagt
     2101 tcagactgga cgcgcgaatc atcgatggag atattcgccg gcatgatgag gggattgcgg
     2161 tcgcccttct cccagaggct gtggatgacg gacgccatca atcggaggac gccccgtgtg
     2221 cgctggaact tcaccagggt ggaccagtcg gtgtagaggc ggtcgaagat ctccggatgg
     2281 atggggtagg ctgccctgat acgtttttcg taatcggcgt cgcgacattc cggggggaat
     2341 tccgcctgct gggtgcggta gaagtcggca aatgcccgtg ccacgacgtc gcggtccttg
     2401 aactgggccg gatcggccag tggttcgaag agccgccgcc tcacgatttc gaaaccttcc
     2461 tcggcactgg ccggccgcca ggaagattcc agccgcccca caacatttct caacctgtcg
     2521 agggcctccc gtccccgctg accgcccacc tctgcgtcat ccgtctgtgc gtggggcgag
     2581 cccgacgtat ccgaagcggg aaggctgatc accagcagac agttccccgc cagtttcgct
     2641 gattcggtca atgcctgggc aaaggtgaat tgcgtctcga aactgccgcc gggaagatcg
     2701 ctctgatcat gaagctgacg cgcataggcg acccattcgt cgataaggat caggcaggga
     2761 ccatacctga cgaataactc acgcagcaca tcgccggggc tggtggcccg ctcgtcatcg
     2821 gcggcaagct gatcaaatgc cttccctgcc tcgctctccc ctccggcagc gtagcccaac
     2881 tgccaggcca gctcgcccca tagggtccgc accaccgtgc cgtcctgttt cctgaccgga
     2941 ttgccggggg atatccgatt gcccaccaga acaacgcggc ggacggacgg cagtgtcgcc
     3001 gttcccgcct cctgcatgac ggcatcgatg ccggcaagct cggttggggc aatcccggat
     3061 aacaggtgat aaagcgccag catcgcgtgg gtcttccccc ctccgaagtt tgtctggagc
     3121 tgcaccacgg gatctgcgct ctgcccggaa agccgccgga ccgcaccgac gagcattctt
     3181 ttcaggcttt cggtcagata ggtgcggcgg aaaaattcta tgggatctcg atactcaggg
     3241 gtgccttcgc ccagacgaac ctgccagaga tcagcggcaa actccgcctg ctgatagcga
     3301 ccgctggcca catctttgtg tggcgtcacc acttcgcgcc agggtttcag atttcccgcc
     3361 gcagcgcttt ctatggccga tcccgcgctt tttcgctttt cgctccgcac ctgttcgtcg
     3421 tatatggtgc gccgtaattc catcttgagt tttcggacgt cttcggcctg cggcgccgat
     3481 accgcagcca gtaaccgctc ggcggaatca agcgcccggt cggcgtcgtc gccggaaaat
     3541 ggttcctggt gagcccattt gttgcgatga tcgcgcagtt cgctcaccag gctccgttcg
     3601 gtgtgcccaa gcgtcttttt gaagacgcta ttccattggt tccaaagtgc agccagcacg
     3661 acggcgatat cgccgaaggg ttcggcctcc gtaccggcaa aacttaattg ctggggagga
     3721 agagacgctt tcagttcgtc aaaccagctt tgttgatact gggactttag ctccctctcc
     3781 acgaagggac gcaacccctc ctttaacagg ctaagcgcct taccgacacg ttcatgattg
     3841 gtaattgcca taaattcact cccgtcttat tcttaagcga accttcttgc ctgtagtgct
     3901 cttgagcatt cgttgtatcc cttctataaa tacctggaaa ctacgtgaat agttatctga
     3961 tttctcaagc ggcatggcca tgcccatccg gcgggcaccc tcaatttttc tgaattctgg
     4021 tatcagttta atcacttcgg aagatgggtt gccgagtcga tccggatttc gatacttaga
     4081 ctttaaattc aaaccggtta actttggtcg ggcatagacc gccgccagta tatccattgc
     4141 gcctaaatac catgactcca attcctggca ggcaatgcga atcagaacat ctgatttccc
     4201 ttcgttcttg cagacatcaa ttaatctcgc tttgatggca cggcaatctg ccccgtcatt
     4261 atcacggata atgacaaaca gctcccggcg aaatgcctta agcttgcgag ggatgctttt
     4321 ctccagatcc tgcttgccct catgggtgat gcagaggaat tcaagatcgg ggataaggcg
     4381 gggcaagaac tcttcgataa acgccttcat cgaaggttcc tccagaagga atacgatctt
     4441 cttcattatt accatgcccc cttgaaaacg ccttgacgcc aaagagtccc gggataatcg
     4501 ccttcggcaa ccagactttt caggatctca ctatcggcgc cgcgatgaat agtcgcgtaa
     4561 ccttccttct tggtcaacca gtatatctcg tccagattca gaccattcat aaactccggg
     4621 gaatgggtcg atatgaaaac ctggccgccc ctccgggcgt aatcgcgaaa ttcttccgca
     4681 agctcctgca atagttccgg gtaaagttga ttttccggtt cttccaccgc cagcagcgga
     4741 aacggattgg gatcatgaag gaggactaga taggcaaaca tcttgatggt tccgtctgat
     4801 acgtagcgag caataaaggg atccttaaaa gccccatcct ggaaacggag caccagccgt
     4861 ccgtcctctg taggcttggc ttcaaccttt tccacaccgg gaatgcgcct cttcatggca
     4921 tcgagaatat cattaaaccg gtcttggtgg tgatcgaaga ggaactgtgc cacctgcgca
     4981 acattatcac cccgggtgga aagatgttcg gcataccctt cctcggcgct gggacgggcg
     5041 tcggcaatgt ggaaatcggt gatatgccag ttctcaatga gattgcggaa ctcggagaca
     5101 acgcgaaact ccttgaactg ccccaatccc ttgatcgcca ggactgtcgg ttcatcaagg
     5161 atatactcct tacgttcctt cttggccccc ggttcaccat acacggcttc attggtaatg
     5221 gctgtcccct tcccacggga aaaatcgaca aaatgccagg gttgaccgcg ctgccccctg
     5281 cggaacttaa gcacttcccg gtccacaaca accttgccgc cttcaaatgc tacggatagt
     5341 tcataagttg ccaatcgctt gcctgtttcc cgaaatttaa gcacaatgga tatcggacct
     5401 tctgtacccc ggctaaccag ttctttaaac ccgccacgtt tggcaacagc ttttgctacg
     5461 ttctgtgcaa gcgcatcctt caggaaggaa aaaacatcga aaaaagtgga tttccccgct
     5521 ccattctcac cgacaagcac ggcaagtctt tttaggtgca tcaatgaggc ctctttgaac
     5581 agccggtaat ttaaaatgtc gatgctctcg atctgcatgc tctgtatttc catcctatcc
     5641 atggttcttt ctccccatca tctacaaaat aacctatatt tcgtcattgt cgaagatatc
     5701 ggcttgcacg cctcgcgatt tttcacttcc cgttgccaac cgcatgattt ctggccagct
     5761 ttgcaccagg ctgttgtatg aaagcgcctc ggcggcgcgt ttcttccgtt cgcacaatgt
     5821 atagagtcga tacgccaatt cacgggcaac ttccgctttc gatcccagct tggtgacaag
     5881 aaccgccgcc gcgccttcac cgcctgtttc aagcgcacgg acaagctgat ggaccatctc
     5941 ccacgccgga atccgtttgt ctttctccgg atcccaagca gcggatagtt cgtccggacg
     6001 gagcagtcgt accctgccgc cagtagatgc gagtattccg tctcgcttca tcccgtcaat
     6061 gctggtggcc ttggcctttg agaggacctc cgccacaccg tactcgcctt cgtcaaagcc
     6121 tgcttgctcg aaccacgcca atgcccagcg gctgtcggca tcaaagtcgc cttcctgctc
     6181 ggccaagacc tcatcgaggg tttgattgat cagcgccagg gcctcccgca ccgtaagcgg
     6241 cttgccctcg gcatcgagga ctttggcgta gcgagtgaag attgccatcc ccgggccgat
     6301 ggccgcctgc gccaagtcta ccggagcgat attgctacgc tgcaaatggg cgagtgccac
     6361 gggtagctcc gctttaagca cgttgatgaa ttcgcgacgt gtggatattg gcgcgtctgc
     6421 tacgcgctta cgg
//
LOCUS       DAOSIM010000111         6432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_10910_length_6432_cov_5.858241, whole genome shotgun sequence.
ACCESSION   DAOSIM010000111 DAOSIM010000000
VERSION     DAOSIM010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10910_length_6432_cov_5.858241"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(303..1472)
                     /gene="bioF"
                     /locus_tag="PLR47_06010"
     CDS             complement(303..1472)
                     /gene="bioF"
                     /locus_tag="PLR47_06010"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HOG12534.1"
                     /translation="MSWQRIEEEIKRINAHGLLREFRYLESAQMPRIKIRGKDVLLLS
                     SNNYLGLCNDERLKIAAKEAIDRYGVGAGGSRLTTGSYDIHRKLEQSIADFKRTEAAM
                     VFNTGYMTNVGVISGMADGDWIIFSDSLNHASIIDGCRLSGAKTVIYRHADPADLEEK
                     ILKHRSAPGLVVSDGVFSMDGDIAPVPELARIAHRFGLLLMVDDAHATGVLGAQGGGT
                     SEYFGLQDVVDIQMGTLSKSLAGEGGFVAGRKLLIDYLKNRARSFIFSTAISPQMVAV
                     SLRAIEIMKQDASARETLLENAGWFRQALRQAGFQVPEGITPIIPVIIGDALRTLQFS
                     LKLLEEGIQISAVRPPVVAVGTSRLRITLMATHTRDDLTFALEKVKSVGGSLGVI"
     gene            1692..2903
                     /locus_tag="PLR47_06015"
     CDS             1692..2903
                     /locus_tag="PLR47_06015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG12535.1"
                     /translation="MRQTMNHETLWTKDFITVTVINFVVFLTHFLLIVTIASYAVDEF
                     HASTDIAGLVAGIFIIGALIGRLGTGRVIEDIGSKKVLIISAFFFIITSALYFAAVNL
                     FLLIVIRLLHGIAFGAASTATGTIIAQIIPPARRGEGISYYSMGAILAVALGPFIGIF
                     LIHHADFRMIFAFTSLLASASFAMSFLVNKPVYEKPAPEPSGAARRFQISDYLEFKAI
                     PISIIILVIGFSYSGLLTFISLYAKEIHLEKAASFYFLVYSIAVFVSRPFSGRLMDIR
                     GANFVVYPCLVLYALGMLLFSQAAQGITLLAAAVLLALGYGSFISCAQAISIRGVPPH
                     RMGLAITTFFIFVDMGFGVGPYVLGALVPLVGYRGLYVLMTFVILASIPLYYFLLGKK
                     WSGRPAQGPEP"
     gene            complement(2976..3107)
                     /locus_tag="PLR47_06020"
     CDS             complement(2976..3107)
                     /locus_tag="PLR47_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12536.1"
                     /translation="MIPDDEDFLTPLPVFSGDFPSSVELLFENRPQTAAGTAISGWN"
     gene            3119..3952
                     /locus_tag="PLR47_06025"
     CDS             3119..3952
                     /locus_tag="PLR47_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG12537.1"
                     /translation="MIIQVDPDKCIGCGTCRADCPTANIAIKDGKAVHGKWCLECGHC
                     LAVCPEKAVLFTGDYDPSEVLEYDDPKKFEIAPDRLLNFVKFRRSVRQYTDEPVSDAD
                     IAKILEMGRYTQTGANTQTLRYIVLTKETLREITPIALKTLAELDIRQVDKKTMRVPS
                     QYLDFQPIWANWHSFYQKKQRDSLFHGAPNALLIVSRTCNEVDGCFNAGHLELMINAL
                     GLGACLMGFGTFAFDRSPELRQKVGIQEGESVIFMMVFGHPKVKYLRTVNRRKVRYEK
                     I"
     gene            4092..>4334
                     /locus_tag="PLR47_06030"
     CDS             4092..>4334
                     /locus_tag="PLR47_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOG12538.1"
                     /translation="MSDTVLKEFKDGVLLLTLNRPESKNSFNKELWESFAAELDAARA
                     DDDVTVVVVTGAGKDFSAGQDLKDYAVSGKAHSYRIT"
     assembly_gap    4335..4442
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4443..4830
                     /locus_tag="PLR47_06035"
     CDS             <4443..4830
                     /locus_tag="PLR47_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOG12539.1"
                     /translation="SLGMAPEFASSYMLQMRIGPQLAAELLFTTEWIDADKALLTGIA
                     TRKFADDELLPCAMAKAAEIAQLPLSSLLETKRCLKTAHRAGIEAALKVEREAMDRLA
                     GGPASIEAMMAFMEKRKPDFRSLKKK"
     gene            5112..5387
                     /locus_tag="PLR47_06040"
     CDS             5112..5387
                     /locus_tag="PLR47_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12540.1"
                     /translation="MPDYRVKISETTDGDLEYHHYLVTAKDETEARRHTMKFMERFID
                     DDNDPEIIENGYTFYNKAVVVRLESIKETTKDKFKEFLLKLHTINMG"
     gene            complement(5406..6197)
                     /locus_tag="PLR47_06045"
     CDS             complement(5406..6197)
                     /locus_tag="PLR47_06045"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOG12541.1"
                     /translation="MKRSLDKGGFGTPVDSGFRVSLLPGKPVLVFILAFCCLFLPAWS
                     PAADKTEAEYAAQRENMVARQIEDRGISDPGVLRAMRAVRRHKFVPESLRGGAYADHP
                     LPIGEGQTISQPYIVALMTEGIRPKSHMKVLEIGTGSGYQAAVLAELCQSVYSIEIVE
                     SLGKRAAKLLSGLYDNVHVRIGDGYQGWPQEAPFDAILVTCAPTKVPRPLEDQLKEGG
                     RMVIPVGESWVQELVVLTKRNGRLERQEIIPVRFVPMVDAKGRKY"
     gene            complement(6233..>6432)
                     /locus_tag="PLR47_06050"
     CDS             complement(6233..>6432)
                     /locus_tag="PLR47_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12542.1"
                     /translation="PDLPRPFRVPLYPLTPLAFIAVCAFMLYASLAYTGRGSWVGVCV
                     LLSGIPLWVWQKKRRTADGHS"
BASE COUNT         1424 a         1792 c         1677 g         1431 t
ORIGIN      
        1 aatcttcagc tttcataata ttctccggta agcatgaaca ggttcttaaa tatcattttt
       61 catcataaaa tgaacatcta gaatttacat gctgtttgtt tgatatgtac cattaatcgt
      121 ccttcgcgtc ctttccacga caatcaaact gataattttg gggtgctgaa tttatttgct
      181 gtgtctttta ttccactacg tatggcttgg gttgaacaat cttaccaatt cccccataac
      241 tcgtcttgga acttcggggc acactataca acgtgttgaa aagaatgcaa gaataaaaac
      301 cgtcagatca cgcccaagct gccgccaacc gatttgacct tttccagagc aaaggtcagg
      361 tcgtcccgtg tatgcgtcgc catgagcgtg attctcagcc tgctggtccc gacggccacc
      421 acgggcgggc gcaccgccga aatctgtatg ccttcttcaa gcagtttgag actgaattga
      481 agcgtccgca aagcgtctcc gatgatcacc ggaatgatcg gcgtgatgcc ttcgggaacc
      541 tgaaatcctg cttgccgaag cgcttgccgg aaccacccgg cgttttccag aagcgtttcc
      601 ctggcggacg catcctgctt cataatctcg atcgcccgaa gagataccgc gaccatctgg
      661 ggagaaattg ccgtggaaaa gatgaagctt ctggctctgt ttttcaggta atcgatgaga
      721 agctttctgc cggccacaaa accgccttcg cccgccagag acttgctgag cgttcccatc
      781 tgaatatcga caacatcctg aagcccgaaa tattccgacg tgcctcctcc ctgcgctccc
      841 agaacgccgg ttgcatgagc gtcatcgacc atgagcagca gcccgaagcg gtgcgcaatc
      901 cgcgccagtt caggcacggg cgcgatgtct ccgtccatgc tgaacacgcc gtcgctgacc
      961 acaaggccgg gtgcggatcg atgcttaaga attttctcct ccagatccgc gggatcggca
     1021 tgtctgtaaa tcacggtttt tgcgccgctg agcctgcatc cgtcgatgat gctggcgtgg
     1081 ttgaggctgt cggagaaaat gatccagtcc ccgtctgcca tcccggagat gacgcccaca
     1141 ttggtcatgt atcccgtatt aaaaaccatc gccgcttcag tccttttaaa atcagcgatc
     1201 gactgttcca gcttgcggtg aatatcatag ctgccggtag tcaggcgcga ccctccggcc
     1261 ccaacgccgt aacggtcaat ggcctctttt gccgcgattt tgagccgttc atcattgcac
     1321 agccccagat agttattgga agataaaagc agtacatcct ttcccctgat tttgatgcgc
     1381 ggcatctgcg cggattccag ataccggaat tcccgaagaa gcccatgcgc attgatgcgt
     1441 ttgatttcct cttcaattct ctgccagctc atcgttgccc catccctgta tgcggttttg
     1501 actcattttc tcactccggc cgatgaaagg caagcgcgta catttccatc ctgtcattga
     1561 cacgcgcaaa ttatgctttt atagtccccc cgcaggaata gcccgcggct tgacaaaccc
     1621 ggcggcgtcc gtcgccgcga cgattgcggt caaagcggcg cagcgctttt cagaaccaca
     1681 ggcaggcatt catgagacag accatgaacc atgaaacgct atggaccaag gattttatca
     1741 cggtcaccgt gatcaatttc gttgtctttc tgacgcattt tctgctgatc gtcacgatcg
     1801 cgtcttatgc cgtggatgaa ttccatgcat ccacggacat tgcggggctt gtcgcgggaa
     1861 tcttcatcat cggcgcgttg atcggaaggc tgggaaccgg gcgtgtgatc gaagacatcg
     1921 gaagcaagaa agttctcatc atcagcgcct tcttttttat cataacgtcg gcactgtatt
     1981 ttgcggccgt caatttattc ctgctgatcg tcatccggct gctgcacggg atcgccttcg
     2041 gagcggccag cacggcaacc ggcaccatca tcgcccagat catcccgcct gcccggcgcg
     2101 gggaaggcat cagctattac agcatgggcg ccatcctggc ggtggccctg ggtccgttta
     2161 tcggcatctt tttaatccat catgcggatt tcaggatgat ctttgcgttt acctcgcttc
     2221 tggcgtccgc cagctttgcc atgtcgtttc tggtcaacaa gccggtctat gaaaaacctg
     2281 cgccggagcc gtccggagca gctcgccggt ttcaaatctc cgattatctg gaattcaaag
     2341 cgataccgat ctccatcatc atcctggtca tcggattcag ctattccggc ctgctgacgt
     2401 tcatctccct gtacgccaag gaaatccatc tggaaaaagc cgccagcttt tattttctgg
     2461 tgtattccat cgccgtcttc gtctcgcggc ctttttccgg acgactgatg gatatccggg
     2521 gcgccaattt tgtcgtttat ccctgcctgg tcctttatgc cctgggcatg ctgctgttca
     2581 gccaggccgc ccaggggatc acgctgcttg cggcggcggt cctgctcgcg ctgggctacg
     2641 gaagttttat ttcctgcgcg caggccatct cgatcagagg ggtgcctcct caccggatgg
     2701 ggctcgcgat tacgaccttt ttcatcttcg tggatatggg gtttggcgtc ggtccgtacg
     2761 tgctgggcgc gcttgtcccc ctcgtgggat accgcggcct gtatgtcctc atgacctttg
     2821 tgattcttgc ctccattccc ctgtattatt ttctgctggg aaaaaaatgg tccggcagac
     2881 cggcacaagg accggaacca tgattgaaca ggtgcctgtc ttgtataaag ggattcatcc
     2941 cgttctttta tcttgacaag ggcaaggccc ccatattaat tccaaccgga aatcgccgta
     3001 ccggcggctg tttgcgggcg gttttcaaaa agcaattcga cggaagaagg aaaatctccg
     3061 gagaatacag gaagcggcgt taagaaatct tcatcatccg gaatcacagg agagtttcat
     3121 gatcatacaa gtggatcccg acaaatgcat cggctgcggc acatgcaggg cggactgccc
     3181 caccgccaac atcgccatca aagacgggaa ggccgttcac ggcaaatggt gcctggaatg
     3241 cggacactgc ctggccgtgt gccccgagaa agcggttctt tttaccggcg actacgatcc
     3301 ttccgaagtt ctcgaatacg acgatccgaa gaagtttgaa atcgcgccgg atcgcctcct
     3361 gaattttgtc aaattccgca gaagcgtccg tcaatatacc gacgaaccgg tgagcgacgc
     3421 ggacatcgcc aaaattctgg aaatgggccg gtacacgcaa acgggcgcga acacgcagac
     3481 gcttcgctat atcgtgctga cgaaagaaac gctgcgggag atcacgccga tcgccctgaa
     3541 aaccctggcg gaactggaca tccggcaggt ggacaaaaaa acgatgcggg tcccttccca
     3601 atacctggat tttcagccca tctgggccaa ctggcattct ttttatcaga aaaagcaaag
     3661 ggattcgctg tttcatggcg cgcccaacgc gcttctgatt gtcagccgca cctgcaacga
     3721 ggtggacggc tgcttcaacg ccggccatct ggagctgatg atcaacgcgc tgggactggg
     3781 cgcctgcctg atgggttttg gaacgttcgc gtttgaccga tcgcccgaat taaggcaaaa
     3841 agtcgggatt caggaagggg aatcggtgat tttcatgatg gtcttcgggc atcccaaggt
     3901 gaaatatctt cgaaccgtca accgcagaaa agtgcgatac gaaaaaatct gatttcagac
     3961 ccgccggctt gcaccgggcg tgacggcgcc cgctgctctt tgcggcctgg tgaacaggga
     4021 cggccggaac ttgcgcaatt gccggggcaa aactcccgga aaaatacatt caaagaaaag
     4081 aggtatcaaa aatgagcgac acggttttaa aggaattcaa ggacggcgtc ctgctgttga
     4141 cgttgaaccg cccggagagc aaaaactcat tcaacaagga attgtgggaa tcttttgccg
     4201 cggagctgga tgcggcgcgg gccgatgacg atgttacggt ggtcgtcgtc acgggggcgg
     4261 gcaaagactt ttccgccggc caggatctga aggattacgc cgtttccggc aaggcgcaca
     4321 gctaccgcat cacgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nncagcctgg gaatggcgcc ggagttcgcc agcagctaca tgctgcagat gcggatcgga
     4501 ccgcagctcg cggcggagct gctgttcacc acggaatgga tcgacgccga caaggccctg
     4561 ctaaccggca tcgctacccg caagttcgcc gatgatgagc ttttgccctg cgcgatggcg
     4621 aaggccgcgg agattgcaca attgccgctg agctcgcttc ttgaaacaaa gcggtgcctc
     4681 aaaaccgctc atcgggcggg catcgaagcg gcattgaagg tcgagcggga ggccatggac
     4741 cgtctggccg gcggtccggc cagcattgaa gccatgatgg ctttcatgga aaagcgcaag
     4801 cccgatttcc gcagcctgaa gaagaaatag ccggatccgg ttttcgcttt tccggcgggc
     4861 aagcattccc gttcctcgcc agggccgtgg tcaaatacct cgcgccaagc tgtcgaggca
     4921 tgaagtgaac gtcatcatat cgcagcaggc tgcggagaat caacgctcgt cccgccaaag
     4981 cgggattgaa aaaacggacg tttccggtgc ggcaaaattt tcatccgctt cccaacggga
     5041 tagttgcctt cggccagtga ttggagtata taccggccgg cagcattcat taaaaacagg
     5101 agaacgatta tatgcctgac tacagggtaa aaatcagcga gacgacggac ggggatctgg
     5161 aataccatca ttacctggtg accgccaaag atgaaacaga ggcaagaaga cacaccatga
     5221 agttcatgga gcgcttcatc gatgacgaca acgatcctga aatcatcgaa aacgggtata
     5281 cgttttacaa caaggccgtg gtcgtccggc ttgaatccat caaagagacg acgaaggaca
     5341 aattcaagga atttcttctg aagctccaca ccatcaatat gggctgagcc gaaagaaatc
     5401 ttgcttcaat actttcggcc cttcgcatcc accatgggca caaagcggac cggaatgatt
     5461 tcctgtcgtt cgaggcggcc gttccttttg gtcagcacaa ccagttcctg cacccaggat
     5521 tctccgacgg gaatcaccat ccggccgccc tctttgagct gatcttccag cggccgggga
     5581 acctttgtcg gggcgcaggt gacgaggatc gcgtcaaagg gcgcctcctg cggccacccc
     5641 tgatacccgt cgccgatccg gacatgaaca ttgtcataca gtccggagag aagctttgcc
     5701 gctctttttc ccagggattc gacaatttcg atcgagtaaa cgctttggca gagttcggcc
     5761 agcaccgcgg cctgatagcc ggatcccgtc ccgatttcca gcactttcat atgagacttc
     5821 ggccggatcc cctccgtcat caaagcaacg atgtagggct gggaaatggt ctgcccttcc
     5881 ccgatcggca gtgggtggtc cgcgtaggct ccgccgcgca ggctttcagg aacaaacttg
     5941 tgccgcctga cggcgcgcat ggcccgaagg acgccgggat cggaaattcc cctgtcctcg
     6001 atttgccgtg caaccatgtt ctctctttga gcggcgtact ctgcttccgt cttatccgcc
     6061 gccggactcc aggcgggtag aaagaggcag cagaaggcca ggataaaaac caaaaccggt
     6121 tttcccggca ggagcgaaac gcgaaagccc gaatccaccg gggtcccgaa gccgccttta
     6181 tcaagacttc ttttcatttt tctccccctc catggtttga tcactttagg ttttacgaat
     6241 gtccgtccgc cgttcgtctt ttcttctgcc aaacccaaag tggaattccc gaaagcagaa
     6301 cgcatacgcc cacccaggaa ccgcggccgg tgtacgccag gcttgcgtac agcatgaagg
     6361 cgcagacggc gataaacgcc aggggcgtca acggatacag cggcacgcga aaaggccggg
     6421 gcaaatccgg at
//
LOCUS       DAOSIM010000112         6368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_11054_length_6368_cov_7.928719, whole genome shotgun sequence.
ACCESSION   DAOSIM010000112 DAOSIM010000000
VERSION     DAOSIM010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11054_length_6368_cov_7.928719"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLR47_06055"
     CDS             <1..371
                     /locus_tag="PLR47_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12543.1"
                     /translation="KQGAVIVDIAIDQGGCCETSRPTTHDDPVFILDGIVHYCVGNMP
                     GAVPRTSTVALANTTLPYGMMIAETGLEKACEFNEGLSKGVNIYEGVCANKNVAESLD
                     LPYTPVHEVMQYPQICASAG"
     gene            complement(492..>816)
                     /locus_tag="PLR47_06060"
     CDS             complement(492..>816)
                     /locus_tag="PLR47_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOG12544.1"
                     /translation="ALLEKVTMHQFGVAAKEGPEMFLADATVYLEFFGIIAIAWQWLR
                     QATAAKKSLAKSPGEADENFYQGKLAAFRYFFGYELPKIQGLSDRLLSSDGLTVKMKN
                     EYFED"
     assembly_gap    817..826
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<827..2250)
                     /locus_tag="PLR47_06065"
     CDS             complement(<827..2250)
                     /locus_tag="PLR47_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG12545.1"
                     /translation="MAEKFISDRNLRFLLYEVNDVESLLKYSRFADHSREVFDMILDT
                     AMRMGKDLFKPLFEEMDRKAPEYVNGEVRVHPRVREIMRAAGDGGWIASTFPYDLGGQ
                     QLPFTIGVMLPTAIFGAANYSAAVYHGLTHGAAGLIASYGTQEMKDAYLPKMIAGEWQ
                     GTMALTEPQAGSSLTDLTTTATPTDQGYCKIKGQKLFISAGDHDGCDNVIHLALVRLK
                     DAPAGIKGISLFIIPKKRIKDDGSLEPNDVNCAGIYHKLGYRGSPITQLAFGENDDCR
                     GWLVGEANKGLAYMFQMMNGARIDVGLAAAVISSAAYYASLEYAKERPQGRKLTSKDP
                     LSPQIPIIEHADIRRMLLFQKAVVEGSLSLLFQCGIYEDLEHVTEGEERENYNLLLEL
                     LTPVAKTYPSEMGILSCSAGLQILGGYGFCEEFPLEQLYRDVRIHPIHEGTTGIQGMD
                     LLGRKMMMKKGKAAQLYLAEVRKA"
     gene            complement(2388..3596)
                     /locus_tag="PLR47_06070"
     CDS             complement(2388..3596)
                     /locus_tag="PLR47_06070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOG12546.1"
                     /translation="MSREVVFVDGMRTAFGTLGKTLSGILGEEMAGLCIKGLVARSKI
                     LEKGGVVDCCFMGSAIGPLTAINGARWPALASGLPESTSASYIEMQCGSAIDSINHAA
                     WKILAGQADVIIAGGYESYSSAPARFPMNTPPFRMIPPLPVAPSLAPSRDPATMNMGL
                     TAEALQKIYSIPRQAQDEFAYRSQMRTKKAIESGFCARDIVPITVPQGKKPPLEFKVD
                     EFPRLETTMEGLAKLPPAFTKEGTVTAGNASGRNDGAAFVLMMTREKAEELGCKPWAK
                     WLSGGDVGVDPKIMGIAPAYAVPQALKRAGIKMSQLDVMECNEAFAVQNLAVAAELEK
                     QTGEKVDWEKWNPNGGAIAYGHPNGASGSRIGIFCMRELMDKGGKYGCFSSCCGGGLG
                     VACIVENLTF"
     gene            complement(3856..4452)
                     /locus_tag="PLR47_06075"
     CDS             complement(3856..4452)
                     /locus_tag="PLR47_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12547.1"
                     /translation="MKKGWMVFALLMISLTVSGAATAQSRKPVLARTVNSMMDVNGDA
                     VVSVTEHAAFWQGRFKDIDIDRDGKLTAAEFEAANKEFFGTMDEDQNGSLVVKEYMAF
                     WCGPKAGVPARVKAVSKKKLDVNRDGKIGDDECVVFWAANFFDVDSNHDGKITTDEFM
                     AAMSRRFKEIDKNRNGWISLEEHTYFMSKNAGTAQKIK"
     gene            complement(4495..>4903)
                     /locus_tag="PLR47_06080"
     CDS             complement(4495..>4903)
                     /locus_tag="PLR47_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG12548.1"
                     /translation="QLPGAVEKFGSAHNYAWPVHLLSTTSRPGAFTPEELEEYRRAFA
                     QPGAFSAMVNWYRAMIQTQPEPPQSFDVAMPMILLWGEDDVAMLSEMADQSMAYCKQG
                     KLIKFPGVSHWIQHEEARKVNALIEELITIPKR"
     assembly_gap    4904..4913
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4914..5345)
                     /locus_tag="PLR47_06085"
     CDS             complement(<4914..5345)
                     /locus_tag="PLR47_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG12549.1"
                     /translation="MDLRERCIETNGIKLHVMESGPSDGPMILFLHGFPEFWYAWRKQ
                     IGYFADRGCLVVAPDQRGYNASDKPAGVAAYNIDELAKDIIGLIDAYGRKQVFLVGHD
                     WGASVSWWVALKYPSRVEKLAILNVPHPKVMARNLFANQRQM"
     gene            complement(5519..>6368)
                     /locus_tag="PLR47_06090"
     CDS             complement(5519..>6368)
                     /locus_tag="PLR47_06090"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG12550.1"
                     /translation="AINPQTGQELMYPAGPAAVRRKVWVVGGGPAGLTAAFEAARLGH
                     QVTLFEQEKETGGNVRYAAKAPHKEVYGRYIQTLTAKCKKKGVEIQTGVEVTEEMVDA
                     GKPDAVILAIGAGKAACPAEGVNASVVCDAWQILDGTVQPKEPAVVIGGGLVGMETAD
                     FLREKGLKNITIVEMLPASPVLALAAHGYMLHKRLRAAGIALMLGTRVKKIEENAVTV
                     EVKGEEKRLEPVAQVIVAIGVTPRTALKDMLAKKGIRHFVVGDAAAPRRIIEATTEGA
                     KAAWEI"
BASE COUNT         1279 a         1907 c         1734 g         1428 t
ORIGIN      
        1 tgaagcaggg cgccgtgatt gtcgatatcg ccatcgacca gggaggatgc tgcgaaacgt
       61 cccgacccac cacgcatgac gatcccgtct tcatcctgga cggcatcgtg cattactgcg
      121 tcggcaacat gcccggcgcg gttcccagaa cgtccaccgt agctttggcg aacaccaccc
      181 ttccctatgg aatgatgatc gccgaaacgg ggctggaaaa agcgtgcgag ttcaacgaag
      241 gtctttccaa aggcgtcaat atttatgagg gagtctgcgc caacaaaaac gtggcggaaa
      301 gtctggacct gccctacacg cccgtccatg aagtgatgca gtatccgcag atctgcgctt
      361 ccgccggata acctcgcgcc gcggccggat accccgggct gaaacgatgc gttttctctt
      421 ttgaaaccgc ttcaaaaaaa tcaggcaggc ccgactgcgg cctgcctgat tcctgaattg
      481 cggatggcct cttaatcttc gaaatactcg tttttcatct tcacggtcag gccgtcgctg
      541 ctaagcagcc ggtcggacaa gccctgaatt ttgggcagct cgtagccgaa gaagtagcgg
      601 aacgcggcca gcttgccctg gtagaaattt tcatcagctt cgcccggact tttggccagc
      661 gactttttgg ccgccgtggc ctgacgcagc cactgccagg cgatcgcaat gatgccgaag
      721 aattccagat agaccgtggc gtcggccaga aacatctccg gaccttcctt ggcggccacg
      781 ccgaactgat gcatcgttac tttttccagg agcgcgnnnn nnnnnnatgg ccttgcgcac
      841 ttccgccaga tacaactggg cggccttgcc ttttttcatc atcatctttc ggccgagcag
      901 gtccatcccc tgaatgccgg ttgtgccttc atgaatcggg tggatgcgca catcgcggta
      961 aagctgttca aggggaaact cctcgcagaa accgtagccg cccagaatct gcaggcccgc
     1021 ggagcaggac aggatgccca tttccgaggg atacgttttg gcgaccggcg tcagcagctc
     1081 cagaagcaga ttatagtttt cacgctcctc gccttcggtc acatgctcca ggtcctcata
     1141 aatgccgcac tggaacagca gcgacagcga cccttccacg acggctttct gaaacaggag
     1201 catgcgccgg atatccgcgt gctcaatgat cggaatctgc ggcgacaacg ggtccttgga
     1261 ggtcagcttc cgtccctgcg gtctttcttt tgcgtattcc agggaagcgt agtaagcggc
     1321 ggacgaaatc acggccgcgg ccagacccac gtcaatgcgg gcgccgttca tcatctggaa
     1381 catgtaggcc agacccttgt tggcttcgcc gaccagccag ccgcggcagt cgtcgttttc
     1441 accgaaggca agctgggtga tgggggagcc ccggtagccg agcttgtgat agatgcccgc
     1501 gcagttgacg tcgttgggct cgagcgagcc gtcatccttg atgcgcttct tgggaatgat
     1561 aaacagcgaa atgcccttga tgccggccgg cgcgtccttg agccggacca gcgccaggtg
     1621 aatcacgttg tcgcatccgt catggtcgcc ggcggagatg aacagcttct ggcccttgat
     1681 tttgcaatag ccctgatccg tcggggtcgc cgtggtggtg aggtcggtga gcgacgagcc
     1741 cgcctgcggt tcggtgagcg ccatcgtgcc ctgccattcg cccgcgatca ttttgggcag
     1801 atacgcatcc ttcatttcct gagtgccgta ggaagcgatc aggcccgccg cgccgtgcgt
     1861 cagaccgtgg taaacggcgg cggaatagtt ggccgcaccg aaaatcgccg tcggcagcat
     1921 cacgccgatg gtgaaaggaa gctgctggcc gcccagatcg tagggaaagg tggaggcgat
     1981 ccagccgccg tcacccgccg cgcgcatgat ctcgcggacc ctgggatgca ccctgacttc
     2041 gccgttgaca tactcggggg ccttgcggtc catttcctca aacaggggtt tgaagaggtc
     2101 cttgcccatc cgcatcgcgg tgtccagaat catgtcgaag acttcccggc tgtgatcggc
     2161 aaagcgggaa tatttcaaaa gggattccac gtcgttgact tcgtaaagaa ggaatctcaa
     2221 gttccggtcg ctgataaatt tttctgccat cctgcactcc tttgtttgct ggaaaaacgc
     2281 aaggcccgta aagagagggc ttcgagattt tgtgaaaaat ctccggagcc attcagcccc
     2341 ggagatgttg ttgcacccgc aagaggatca tcgcggagga aaaccgctta gaacgtcagg
     2401 ttttccacga tgcaggccac gcccaggccg ccgccgcagc aggagctgaa gcagccgtac
     2461 ttgcctccct tgtccatcag ttcccgcatg cagaaaatgc cgatgcgcga tccggaagct
     2521 ccgttgggat gaccataggc aatggcgccg ccgttgggat tccatttttc ccagtcaact
     2581 ttttcgccgg tctgcttctc cagctccgcg gccactgcca ggttctgcac ggcgaaggct
     2641 tcgttgcatt ccatgacatc caactggctc atcttgatgc cggcgcgctt cagcgcctgg
     2701 ggcacggcat aggcgggggc gatccccatg atcttgggat cgacgccgac atcgccgccg
     2761 gacagccatt tggcccaggg cttgcagccc agttcttcgg ccttttccct ggtcatcatc
     2821 agcacgaaag ccgcgccgtc gttgcggccg gaggcgttgc cggcggtgac ggttccttct
     2881 ttcgtaaacg cgggaggaag cttggccaga ccttccatcg tcgtctctaa acgcggaaat
     2941 tcgtccacct tgaactccag cggaggtttc ttgccctgcg gaaccgtgat cggaacgatg
     3001 tcgcgcgcgc agaagccgga ctctatcgct tttttggttc tcatctggct tcggtaggcg
     3061 aattcatcct gcgcctgacg cggaatgctg tagattttct ggagcgcttc cgcggtgagg
     3121 cccatgttca tggtcgcggg gtcgcgggaa ggggcgagcg acggcgcgac cggcaacggg
     3181 ggaatcatgc ggaagggcgg cgtgttcatc gggaagcggg ccggtgcgct gctgtaagat
     3241 tcgtagccgc cggcgatgat gacgtcggcc tggccggcca gaatcttcca ggcggcgtgg
     3301 ttgatcgaat cgattgccga accgcactgc atttcaatgt aggaagccga ggtgctctcg
     3361 ggcaagccgg acgccagagc aggccagcgg gcgccgttga tggccgtgag cggtccgatc
     3421 gcggaaccca tgaaacagca gtccaccacg ccgccttttt ccagaatctt tgatcttgcc
     3481 acaaggcctt tgatgcacag gcccgccatt tcctcaccca ggatgccgct cagggtcttt
     3541 cccagcgtgc caaacgccgt tctcatgcca tccacaaata cgacttctct actcataacc
     3601 cctcctctgt atttttgttt tgtaaacgat gaaatcgttt gctcgaatgt tgctcaagat
     3661 ccgcgcagcc caacagcgcc tcttcttttg gagcggtatc atacatgaaa catgacgtca
     3721 agaaatatac aacgactgtt gtaaaaatcg cgatggaaaa gcaagggcgg aaagattccg
     3781 cccttgcatg aaaaccggtt ttttacagcc tgcggcaaaa accttcattc atgccggaac
     3841 atataaacat ttgttttatt ttattttttg tgcggttccg gcatttttgg acatgaaata
     3901 ggtatgctcc tcaagggaga tccatccgtt tcggtttttg tcaatctcct tgaaccgcct
     3961 ggacatggcc gccataaatt catccgtggt aattttgccg tcgtggttcg aatccacgtc
     4021 gaaaaaattt gccgcccaga aaacaacaca ttcatcgtcg ccgattttac cgtcccggtt
     4081 gacatccagt tttttctttg aaactgcttt gaccctggcg ggaaccccgg ctttcggccc
     4141 gcaccagaag gccatatatt ccttgaccac cagcgaaccg ttctgatcct catccatggt
     4201 tccgaaaaat tctttattgg ccgcttcaaa ctcggccgcc gtcagtttgc cgtcgcggtc
     4261 gatgtcgatg tctttaaacc gcccctgcca gaaagccgcg tgctccgtca cgctgacaac
     4321 cgcgtcgccg ttgacatcca tcatggaatt gaccgtccgg gccaacacgg gttttcggga
     4381 ttgagccgtc gccgcaccgg aaaccgtcaa cgaaatcatt aacaaagcga aaaccatcca
     4441 tccttttttc ataaaagcct cctcaataaa ataatgaaac agcggggtca tattctaacg
     4501 cttcggaata gtgatcaatt cctcaatcag cgcattaacc tttctggctt cttcatgctg
     4561 aatccagtgc gagacaccgg gaaatttgat gagctttccc tgtttgcagt aggccatgct
     4621 ctgatcggcc atctccgaaa gcatggcgac atcgtcttcg ccccacagca aaatcatggg
     4681 catcgccacg tcaaagcttt gcggcggctc gggctgcgtc tgaatcatgg cccggtacca
     4741 gttgaccatc gcggaaaaag ctcccggctg cgcaaacgct ctgcggtact cttccagttc
     4801 ttcaggcgta aaggcgccgg ggcggctcgt ggtcgaaagc aaatgcactg gccaggcata
     4861 attatgggcc gagccgaatt tttccaccgc gccgggcagt tgannnnnnn nnncatctgc
     4921 cgctgattgg caaagagatt tctggccatg accttgggat ggggaacgtt caaaatggcc
     4981 agtttttcaa cacggctggg atatttcaac gccacccacc acgacacgga cgctccccag
     5041 tcgtggccga ccagaaaaac ctgtttgcgg ccataggcgt cgatgaggcc gataatgtcc
     5101 ttggccagtt catcaatgtt ataggccgca acgccggccg gcttgtcgct tgcattgtag
     5161 ccgcgctgat cgggcgcgac caccaggcag ccccggtcgg caaaataccc gatctgcttg
     5221 cgccaggcat accagaattc cgggaagcca tggagaaaca aaatcatcgg accatcggag
     5281 ggcccggatt ccataacgtg cagtttgatg ccgttggtct caatgcaacg ctcacgcaaa
     5341 tccatctcag atgtctcctt ttgttgttag cggttggcga aaaagtttat tgctcaatcg
     5401 gcagggaacg gtcgcgaccg ttccctgcta tccaccatcc actattcacc gcggcgtttt
     5461 ttgttatgag ctgcgagtct ttccccatag cctctcgccc attgcctata gcctgttttt
     5521 aaatctccca ggcggctttc gccccctccg tcgtcgcttc gatgatcctt ctgggcgcgg
     5581 cggcgtcacc cacgacaaaa tgacggatgc cttttttcgc cagcatgtct ttcagcgccg
     5641 tgcggggtgt aacgccgatg gccacaatca cctgggcaac cggctccaat cttttttcct
     5701 ctcccttgac ctcgaccgtc accgcatttt cttctatctt tttcacgcgg gttcccagca
     5761 tcagggcaat gccggccgcc cgaaggcgct tgtgcagcat gtagccgtgc gcggccagcg
     5821 ccagaacggg tgaagcaggc agcatctcca cgatggtgat gttctttaag cccttctccc
     5881 gcagaaaatc ggcggtttcc atccccacca gcccgccgcc gatcaccacc gccggctctt
     5941 tgggctgtac ggtcccgtcc agaatctgcc aggcgtcgca aaccacggat gcgttgacgc
     6001 cctcggccgg gcaggcggct ttgccggcgc cgatggccag aatgacggca tcgggtttgc
     6061 ccgcatccac catctcctct gtgacttcga cgcctgtctg gatttccacg cctttctttt
     6121 tgcattttgc cgtgagggtc tgaatgtagc ggccgtacac ctctttgtgg ggcgctttgg
     6181 cggcatagcg gacatttccg cccgtttcct tttcctgctc gaagagggtt acctgatgac
     6241 caagacgcgc ggcctcgaaa gcggcggtca gtcccgccgg cccgccgcct accacccaca
     6301 ccttgcggcg aacggccgcc ggacccgccg gatacatcag ttcctgaccg gtttgcggat
     6361 tgatggcg
//
LOCUS       DAOSIM010000113         6365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_11061_length_6365_cov_6.365927, whole genome shotgun sequence.
ACCESSION   DAOSIM010000113 DAOSIM010000000
VERSION     DAOSIM010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11061_length_6365_cov_6.365927"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PLR47_06095"
     CDS             complement(<1..829)
                     /locus_tag="PLR47_06095"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF036305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HOG12551.1"
                     /translation="MNIDKTILSEENLRKIKALNNPHVERIVEEYAALCKPAKVSVIT
                     DSFADIGYIRQMALDLGEEKKLAMPGHTIHFDSFYDQGRDKKNTCLLVTQDMKVSKII
                     DVKDREEGLTEIRGIMDGIMKGKECFVRFFCLGPLHSRFSIAALQITDSAYVSHSEDL
                     LYRVGYEHFKTLNGSDDFFYLVHSAGELDERGNTKNIKDRRIYIDVIGKKVLTVNNQY
                     AGNSVGLKKLALRLAIYKANKEDWLTEHMFIMGVKPEGRKRVTYFTGAFPSACGKTST
                     "
     gene            complement(1151..1747)
                     /locus_tag="PLR47_06100"
     CDS             complement(1151..1747)
                     /locus_tag="PLR47_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12552.1"
                     /translation="MKKFFFGMIFLALVVLTPISAMADVDVKIGINIPLPPPIIFPAP
                     PEVVVIPETYVYAVPDVDADIFFYGGWWWRPWEGRWYRSRYYDSGWNYYDRVPSFYRG
                     VPPGWKNDYRDRRWKGREWDQRRIPSGDAQKNWRSWKRNKHWEKENHWGVREMDRRPP
                     GQQKKYQDRYDGPPRGGGPGNGPKGGPGKGRPDKGNKR"
     gene            complement(1821..2192)
                     /locus_tag="PLR47_06105"
     CDS             complement(1821..2192)
                     /locus_tag="PLR47_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOG12553.1"
                     /translation="MQFTFAHNNFNVLNLEKSLAFYREALGLKEARRYEPPDGSFVLV
                     YLGDGQTAHQLELTWMRERTDPYSLGDNEFHLAFRVDDFAAAHALHEKMGCICFENKK
                     MGIYFINDPDDYWLEIVPAKR"
     gene            complement(2244..3401)
                     /locus_tag="PLR47_06110"
     CDS             complement(2244..3401)
                     /locus_tag="PLR47_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOG12554.1"
                     /translation="MNNFTFYNPTKILFGEGRIKDIAEEIPMDAKILMTYGGGSIKRN
                     GVYDEVRAALQGRAMEEFEGIEPNPTYETLMRCVEMARKNKIDFLLAVGGGSVIDGTK
                     FIAAAVPYEGDPWEIVATYGKKAKGAMPFGTVLTIPATGSEMNNGAVITNRALNAKLP
                     FMHPSLFPKFSVLDPAKTFTLPPEQIGNGVVDTFVHVTEQYLTFPVNGKLQDRFAEGL
                     LLTLIEEGPRALAEPENYDVRANLMWCATLGLNGLISVGVPQDWATHMIGHELTALRG
                     LDHGKSLAVVLPVLLKIRAGEKKEKLLQYAERVWGIAAGDEAARIDAAIEKTRDFFES
                     MKVPTRLCAYNIGADVIPEVVENLKAHGMVKLGETRDITPELVESMMKDCL"
     gene            complement(3524..4450)
                     /locus_tag="PLR47_06115"
     CDS             complement(3524..4450)
                     /locus_tag="PLR47_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12555.1"
                     /translation="MQTPFRIHDLILIVVVVLTMAVAVIFPDFGSRFQAFPVYCLMVN
                     FFLSYLSIDPADVWKAFRDHIREILGFTVLKLALLPVVLYGVFYLVAPDYALAALLLT
                     GVSTGVVSPMISNMVKGNSSLVLLVVVITSALAPFTLPALVKIVIAKEAAISFWAMLR
                     LLATVIFVPIALVEIIRCLTPRLIAPILKIQFPVSLLMFALINLGVFYRYAPFFQEKP
                     SVIIMATVVVFVLAAIYCAVGIFFFRKGPLHNHLAGAVMLGNINNVLVIVFSSQFFGP
                     VEPLVAAMYMIPFFVIVIPLRYYRYRKIRHRS"
     gene            complement(4515..4898)
                     /locus_tag="PLR47_06120"
     CDS             complement(4515..4898)
                     /locus_tag="PLR47_06120"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HOG12556.1"
                     /translation="MYNLLADAVIIVHFLFILFVAAGGLLILRRPRMAWLHLPAACWG
                     AFIEFAGWICPLTPLENHLRRLGGEAPYTGDFVVYYLVPVIYPAGLTPLTQYILGGLV
                     VAVNLVIYGYVLYRRFLRRAQASDQ"
     gene            5017..5883
                     /locus_tag="PLR47_06125"
     CDS             5017..5883
                     /locus_tag="PLR47_06125"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HOG12557.1"
                     /translation="MFGWLTKKRRKKILEAPFPFEWENIIQKRMAHYRMLNEDEQKRL
                     CERIAVFMAEKNWEGCGGLELTDEIRVTISAMACLLILNLPRQGYENVESILVYPSER
                     VLPEPKIGFFETALAPLETPGPISGQAFEQGPVILVWDAVAESIRYAGSGYNVVYHEF
                     AHKLDMQDGVADGTPRLRDRAEYRDWVKTCSREYQKLLKDVERGKRTFLDDYGAKDEV
                     EFFAVSTEHFFDQPLRMRRAAPELYRVLKNYYRQDPAAREGQYAAAREEQTRETGSAF
                     AKAPDAKKGESE"
     gene            5880..6227
                     /locus_tag="PLR47_06130"
     CDS             5880..6227
                     /locus_tag="PLR47_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12558.1"
                     /translation="MIDYILRHEAYVRDVLAGHSEKETLAELLAYHDKQISWVQQERL
                     AHLITMMFVCLFFLLAFGWTLIHFTAPYILLTALLLILSAAYILHYYRLENSVQRWYS
                     LSAQIREKLLQSK"
BASE COUNT         1525 a         1834 c         1636 g         1370 t
ORIGIN      
        1 ccgtcgaggt tttgccgcag gcgctgggaa acgcgccggt aaagtaggtc acgcgcttcc
       61 tgccttccgg cttcacgccc atgatgaaca tatgttcggt gagccagtcc tctttgttgg
      121 ccttgtagat cgccagacga agcgccagtt tcttcaaccc gacgctgttg cccgcatact
      181 gattgttgac ggtcagcact tttttcccga tcacgtcgat gtaaatcctg cggtccttga
      241 tgtttttggt gttgccccgc tcatcgagtt cgcccgcgga atggaccagg tagaaaaaat
      301 catccgaccc gttcagggtc ttgaaatgct cgtagccgac ccgatagagc aaatcttcgc
      361 tgtgggaaac ataagcggaa tcggtgatct gcagggcggc gatggaaaaa cgggaatgga
      421 gcggtcccag gcagaagaac cggacaaaac attccttgcc cttcatgatg ccgtccatga
      481 ttccgcggat ttccgtgagg ccttcctccc ggtctttcac gtcgatgatc ttggacactt
      541 tcatatcctg cgtcaccagc aggcaggtgt ttttcttgtc gcgcccctgg tcgtaaaaac
      601 tgtcgaaatg aatcgtgtgg ccgggcatgg ccagcttttt ttcttcgccc agatccaggg
      661 ccatctggcg gatgtagccg atatccgcga aactgtccgt gatcacggat actttcgccg
      721 gcttgcataa cgccgcatac tcctcaacga ttctttccac atgggggttg ttgagggctt
      781 taatcttccg cagattttct tcgctcaaaa tagtcttatc gatgttcaat tgctcactcc
      841 ctgaattgat ttgttgttcc ttccgttcct gaacacaaca cggcgcagtg atttgaggac
      901 tgaagagggt atcgccggca tcctgaaaac tacggtcagc ggatcgcaaa cccatttacg
      961 cggcagactc taaaggacaa tcccataaaa atcaatcgat ttttacattg cggacaactc
     1021 ccggggcgcc ctttgccgga tcggaaaaaa atcgatcggc agaaaaaaac cgatccggaa
     1081 aacaagcggc aagccgaccc gacagcccgt ggtttgcgca ggcggcccgg ccggcagccg
     1141 aataagacac ttatcgttta ttgcctttgt ccggacggcc ttttcccggt ccgcccttgg
     1201 ggccgttgcc gggaccacct cctcttggcg gcccgtcata gcggtcctgg tactttttct
     1261 gctgtccggg aggacgacgg tccatctccc tcacacccca gtggttttcc ttttcccagt
     1321 gcttgttgcg cttccagctt cgccagtttt tctgcgcgtc gccggaggga atacggcgct
     1381 gatcccactc acggcctttc caacggcggt cccgataatc gtttttccac cccggcggca
     1441 ccccccggta aaacgagggc actctgtcgt agtagttcca gccactgtca tagtaacggg
     1501 atcgatacca gcgcccctcc cacggccgcc accaccagcc gccgtaaaag aagatatccg
     1561 catcgacgtc gggaaccgcg taaacatagg tttccggaat cacgaccacc tccggcggcg
     1621 ccggaaaaat gatcggcggc ggaaggggga tattgatccc tattttgaca tccacgtccg
     1681 ccatggccga aatcggcgtc aacacgacca gcgccaggaa aatcatcccg aagaaaaact
     1741 ttttcattgt actgtctcct ttctcaagcg atgtcgcccc tccggtcacc gaaaagggcg
     1801 gcttgaccca cggttagaac ctatcgtttt gcaggaacga tttcaagcca ataatcgtcc
     1861 ggatcgttga tgaagtatat ccccatcttt ttgttttcaa aacagatgca acccattttt
     1921 tcatgcagcg cgtgcgccgc ggcaaaatca tccacccgga aagccagatg aaactcattg
     1981 tcccccaaac tgtatggatc cgttcgctct ctcatccagg tcagctccag ttgatgcgct
     2041 gtttggccgt cgccgagata caccagaaca aaactgccgt ccggcggttc ataacgccgc
     2101 gcttccttca acccgagcgc ctcacgataa aaagccagac ttttttccag attcagcacg
     2161 ttgaaattgt tgtgggcaaa agtaaattgc atatcctgat cctctttatg ctgttttcat
     2221 gaaagcaaac gacccgatcc cgactacagg caatccttca tcatcgattc caccagctcc
     2281 ggcgtgatat ccctggtctc acccagtttg accatgccgt gcgctttcaa attctcaacg
     2341 acttccggaa tgacatccgc gccgatgttg tacgcgcaaa gacgcgtcgg caccttcatg
     2401 ctttcaaaga aatcgcgcgt tttttcaatg gcggcgtcga tgcgcgcagc ctcatccccg
     2461 gccgcgatcc cccacacgcg ttcggcgtat tgcagaagct tttccttctt ctcccccgcg
     2521 cggatcttca gcagcaccgg cagcaccacc gccagggatt ttccgtgatc caggccgcgc
     2581 agcgccgtca gttcatggcc gatcatatgc gtcgcccagt cctgcggaac cccgacactg
     2641 atcagtccgt tcaggccgag cgtggcgcac cacatcaggt tggcgcgcac gtcataattc
     2701 tccggttcgg ccaaggcccg cggcccttcc tcgatcaggg tgagcagcag gccttccgcg
     2761 aaacgatcct gcagctttcc gttgacggga aacgtaagat actgctcggt cacatggaca
     2821 aatgtatcca ccacgccgtt gccgatctgc tccggcggaa gcgtgaaggt ctttgcgggg
     2881 tccagcacgg aaaattttgg aaacagcgac ggatgcatga aaggaagctt ggcgtttaag
     2941 gcgcgattcg ttatgaccgc tccgttgttc atctcggatc cggtcgccgg aatcgtcaaa
     3001 accgttccga agggcatggc cccctttgcc ttcttgccgt aggttgcgac aatctcccag
     3061 ggatcgcctt catagggaac ggcggccgcg ataaattttg tgccgtcgat cacggaaccg
     3121 ccgcctacgg ccagcagaaa atcaattttg ttttttcgcg ccatctccac gcaccgcatc
     3181 agcgtctcat aggtcggatt gggctcaatg ccttcgaatt cttccatcgc tcggccctga
     3241 agcgccgccc tgacctcatc atatacgccg ttgcgcttga tgctgccgcc tccgtaggtc
     3301 atcaggatct ttgcatccat tggaatttct tcggcaatat ccttgatcct gccctcgcca
     3361 aacagaattt tcgtcgggtt gtaaaacgta aaattgttca tgtgactccc tccaggttga
     3421 acaagtattg cggtgaagaa aatatactgg aattattccc tggtttcaat ggaaaagaag
     3481 ggggaacacc cttcagccct gacgcataaa aaatacacac cgcttatgac cgatgcctga
     3541 ttttgcggta ccggtagtag cgcagcggaa tgacgatgac gaaaaaagga atcatgtaca
     3601 tggcggcaac cagcggttcg acgggaccaa aaaactggga ggagaagacg atcacgagca
     3661 cgttgttgat gtttcccagc atcacggcgc cggccagatg attatgcagg ggccccttcc
     3721 ggaagaaaaa tattccaacg gcgcagtaaa tcgccgccag gacaaacacg acgacggtcg
     3781 ccatgataat cacggacggc ttctcctgaa aaaaaggcgc atagcggtaa aaaacgccga
     3841 ggttgatcag ggcaaacatc aacagtgaga ctggaaactg aatcttcaga atcggcgcga
     3901 tgagccgggg cgtgaggcag cggatgattt cgaccagggc aatcggaacg aaaatgacgg
     3961 tcgcaagcag ccggagcatg gcccagaagg aaatggccgc ttccttcgcg atcacgatct
     4021 tgacgagcgc cggcagcgta aacggcgcga gagcggaggt aatgaccacg accagcagaa
     4081 ccagcgacga gttgcccttg accatgttgg aaatcatcgg cgaaaccacg ccggtggaaa
     4141 cgcccgtcaa aagcagggcg gcaagcgcgt aatccggagc gaccagataa aacacgccgt
     4201 aaaggaccac cggcagaagc gcgagcttca gcaccgtaaa acccaggatt tcccggatgt
     4261 ggtccctgaa cgctttccag acatccgccg gatcaatcga cagatagctt agaaaaaaat
     4321 tcaccatcag acagtaaacg ggaaacgcct ggaaacggga gccgaaatcg gggaaaataa
     4381 ccgcgacagc catggtcagg accacgacga cgatcagaat caaatcatgg attcgaaacg
     4441 gagtttgcat gggtttggga tatcagacag tgcccgcctt atcaacctct ttcacctttg
     4501 caacttcatg cccgtcactg atcggacgcc tgcgccctgc gcaagaaacg cctgtataaa
     4561 acatagccgt agatcaccag gttgaccgcg acaaccagtc cgccgagaat gtactgagta
     4621 aggggcgtga gtcccgccgg ataaatcacc gggaccagat agtaaacgac aaagtctccc
     4681 gtgtagggcg cttcaccgcc cagccgtcgc agatgattct ccagaggggt cagcggacaa
     4741 atccatccgg caaattcgat gaaagccccc cagcaggcgg cgggaagatg cagccaggcc
     4801 attcgcggcc ggcgaagaat aagcaggccg cccgccgcga cgaaaaggat aaacaaaaaa
     4861 tgaacaatga tgaccgcatc ggccagcaga ttgtacataa ccttccctgc ccccgcccca
     4921 ctatagcgcc gcccggcgtt tggttcaacg ataaaccgct gtttttcata tccctgtttt
     4981 tatggtacaa agacaaccgt tgaacggggg accgcgatgt ttggctggct gacgaaaaaa
     5041 cggcggaaaa agatcctgga ggcgcctttt cccttcgaat gggaaaacat cattcaaaag
     5101 aggatggcgc attaccgaat gctcaatgaa gacgaacaaa agcgtctttg cgaacggatc
     5161 gccgtgttca tggctgaaaa aaactgggaa gggtgcggcg gccttgaact cacggatgaa
     5221 attcgcgtca ccatttccgc catggcctgt cttctgatcc tgaaccttcc ccgccagggc
     5281 tacgaaaatg ttgaatccat cctggtctat ccttccgaac gggtcctgcc cgaaccaaag
     5341 atcggttttt ttgaaaccgc gctcgcgccg ctggaaaccc cgggccccat cagcgggcag
     5401 gctttcgaac agggtccggt catcctggtc tgggacgcgg ttgccgaaag catccgttat
     5461 gccgggagcg gctacaacgt ggtttatcat gaattcgccc ataaactgga tatgcaggac
     5521 ggcgttgcgg acggcactcc ccgtttaagg gacagagcgg agtaccgcga ctgggtcaaa
     5581 acctgttcgc gtgagtatca aaaactcctc aaagacgtgg aaagaggaaa aagaacgttt
     5641 ttggacgact atggcgcaaa agacgaggtg gaatttttcg ccgtctccac ggaacatttt
     5701 ttcgaccagc cgctgcgcat gaggagggca gcgccggagc tgtaccgggt tttgaaaaat
     5761 tactaccgcc aggaccccgc cgcccgcgaa ggccaatatg cggcggcgcg ggaagaacaa
     5821 acccgtgaaa ccggatcggc cttcgcaaaa gcgccggacg caaaaaaggg agaatcggaa
     5881 tgatcgacta tatcttgcgg catgaagcct acgtcaggga tgtgctggca ggccattcgg
     5941 aaaaagaaac gcttgccgaa ctgctggcct atcacgacaa gcagatttcc tgggtgcagc
     6001 aggaacgcct ggcccatctg atcacgatga tgtttgtctg tcttttcttt cttctggcct
     6061 tcggatggac gctcattcat ttcacggcgc cgtacatcct gctcaccgcg cttcttctga
     6121 ttctttcggc cgcctacatt ctgcattact accgtctgga aaacagcgtt cagcgctggt
     6181 attccctgtc agcccaaatc cgagagaaac tgcttcaatc aaaataaaca aaataaaaag
     6241 ccccttgtcc ggacggacaa ggggctttac tttttaccct atgacctatc gcctatagcc
     6301 gatagccttt tttacattcc cattccggga taaccgcctc cgggaggcat accgggcatt
     6361 ccgcc
//
LOCUS       DAOSIM010000114         6357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_11077_length_6357_cov_7.967629, whole genome shotgun sequence.
ACCESSION   DAOSIM010000114 DAOSIM010000000
VERSION     DAOSIM010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11077_length_6357_cov_7.967629"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PLR47_06135"
     CDS             <1..772
                     /locus_tag="PLR47_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HOG12559.1"
                     /translation="FSFQVTELVKVAMVLFLAHLLTRKVHHLKKFSRGVLIPLSVTAV
                     VMGLIILQPDFGTVVIMMSILLLMLSLAGSRITHLMFLGAAFIPVGIWLIMSKGYRMA
                     RLTAFLDPWKDADKTGFQLIQSLISFGSGGVTGQGIGDGMQKLFYLPEPHTDFILAVI
                     AEESGFIGVALIIVLFIFFMLRGFMIAFRAPNLFGTLLAAGLTMLIALQAFINIAGVM
                     GLIPLKGLALPFLSYGGSSFVMSMMTVGILLNISTQRT"
     gene            814..1920
                     /gene="murG"
                     /locus_tag="PLR47_06140"
     CDS             814..1920
                     /gene="murG"
                     /locus_tag="PLR47_06140"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOG12560.1"
                     /translation="MRIAIAGGGTGGHLFPGIAVAEEFLKRDPQNRVIFIGTRRGIEH
                     RLLGTLGYELKLIDVEGLKGRGLKALIKGVYAIPNSMIQSGRILAEFRPDAVLGVGGY
                     ASGPAVLAARLMGLPTAIAEQNALAGITNRILGKFVDQIFLTYEQSRGRFDVRKVRVT
                     GNPVRAAFVAGLVAAPTSRAFRQILVFGGSQGAAAINKTVVAMLPMLQGMRRRVRVVH
                     QTGERDLAAVRQAYGKAGVEADVQPFIVDMASAYASADLIICRAGATSLAEITAAGKA
                     SVLIPFPYAANDHQTENARAMVRAGAAAMIPESGLTAGTLYLLVETLLEDGEKLRGME
                     EKSKALGRPGAAAEIVDACLRLGAKQKNTQERGT"
     gene            1917..3329
                     /gene="murC"
                     /locus_tag="PLR47_06145"
     CDS             1917..3329
                     /gene="murC"
                     /locus_tag="PLR47_06145"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOG12561.1"
                     /translation="MSKDQETGFMQRKVKHIHFVGIGGIGMSGIAEVLLNLGYGISGS
                     DVQSSDTTKRLQQLGAAVAVGHTAENIGGADVVVTSTAVKADNPEVLEAHRKNIPVIP
                     RAEMLAELLKMKFSVAVSGSHGKTTTTSMISTLLAEGGLDPTMVIGGKLASIGSNARL
                     GGGDILVAEADESDGSFLKLSPTIAVITNIDREHLDYYPGIEEIKDAFLKFANMVPFY
                     GCTVMCGDNEHVRAISPSIKRRVITYGITHPADYTAGDIRPSGSKTRYALSYKGESLG
                     DVELAVPGLFNVYNSMAAIAVGRELGLEPAILRKGLVAFSGVQRRLEIKGEAGGITVV
                     DDYGHHPTEIMATLAAARQTHKGRLIVVFQPHRFTRTKALFDEFTRAFTDADVLVLND
                     IYPASEAPIPGVHSAALWVAIKEGGHPCVEYIAQPQGTVEFLLANARSGDTVLTLGAG
                     SVYKIGEAFLERLNEEALPS"
     gene            3326..4252
                     /gene="murB"
                     /locus_tag="PLR47_06150"
     CDS             3326..4252
                     /gene="murB"
                     /locus_tag="PLR47_06150"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOG12562.1"
                     /translation="MTGPANIAAALKGANIGRVRFNEPMSRHASLKLGGRVDALVLPE
                     HEAQLEEAVRRLREHRIPFLPVGNLTNILVRDGGYRGALISLRGLDRAVCAPANDGRF
                     WIDAQAGAPLGRIVALAAESELTGLEFCAGIPGSVGGAVWMNAGAFGAEIKDVLVFAD
                     LIDDGGERKTLRREEIAFSYRKSNLPANVIICGARFALSKGRSEQIRDRVAEIMKWRQ
                     EKHPLQYPSAGSVFKNLPGMPAGRLLEELGLKGRRLGDVQISKKHANFMINKGQGTAS
                     DMVKLIRLVQEMVQREKGLFLETEIVVIGEEW"
     gene            4246..5088
                     /locus_tag="PLR47_06155"
     CDS             4246..5088
                     /locus_tag="PLR47_06155"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOG12563.1"
                     /translation="MVSQKIKVNLEAKKNRLRRRLIGALNESLGALALLAMAVAASAV
                     LIYLYNALLSAPGLELKEISVRGVKELTEKDILELAKIQPRCNLLAVNTDAVARRITA
                     NPWVRKIYVGRELPGRLVLDVRERTPIALVKQTGNFYLMDAEGVVFKKLSRTDDVDLA
                     ILTGVNLGKAPSPLLAEALKLLEILSADSYASLGTISEIHIDEVFGLSVLTDRGLHLK
                     LGRDNFTGKVRQLDVVLADLEKRGMKNGRLFVDLADISKVTVQRKDAPGKAQEQKKGP
                     QYRI"
     gene            5113..6339
                     /gene="ftsA"
                     /locus_tag="PLR47_06160"
     CDS             5113..6339
                     /gene="ftsA"
                     /locus_tag="PLR47_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOG12564.1"
                     /translation="MAKKSNVLVGIDIGTTKTIAIVGEVKTTGIDIIGMGITPAKELR
                     KGGVVNIDSTVEAIKKAVEDAEHMSGCRINSAYVGIAGSHVKGQNSLGIVAVKGREVG
                     EDDLQRALEASKAIAIPVDREILHTLPQNYVVDGQDGIRDPVGMSGVRLEAKVHIVTG
                     AAASIQNVVKSVNRVGLDIEDVVLEQLAASEAILSADEKDLGVALIDIGGATTGMAIF
                     AEGSIKHTATLPVGGNFLTSDIATGLRTPFAEAEKIKLNHGCAMTSMIPKEDVIEVPS
                     VGGREARMVSRQILGRIVEPRMDEILNMALKEIVRSGYEDLLAAGIVLTGGSSLLPGI
                     NEMAEQIFDMPVRLGCPAGIGGLSDVANSPAFAVGVGLIVYGSKNIPGDSVYRKTGNA
                     FGEYFKTLKKWFLEFF"
BASE COUNT         1308 a         1725 c         2092 g         1232 t
ORIGIN      
        1 gttttccttt caggtgaccg aactggtcaa ggtggccatg gtgcttttcc tggcccacct
       61 gctgacgcgc aaagtgcacc atttgaaaaa attcagccgg ggtgtcctga ttcccctgtc
      121 ggtgacggcc gtggtcatgg gactgattat tcttcagccg gatttcggca cggtcgtcat
      181 tatgatgtcg attctgcttt tgatgctgtc cctggccggc tcccgcatca ctcacctgat
      241 gtttctgggc gcggccttta tccccgtcgg catctggctg atcatgagca agggataccg
      301 catggcgcgg ctgacggcgt ttctcgatcc gtggaaagac gcggacaaaa ccggtttcca
      361 gctcatccag tcgctgatct ccttcggttc gggcggcgtg acggggcagg gcatcggcga
      421 cggcatgcag aaactgtttt atctgccgga gccgcatacg gattttattc tggcggtgat
      481 cgcggaggaa agcggtttta tcggtgtggc tcttataatt gtcctgttca ttttcttcat
      541 gcttcggggc tttatgatcg cttttcgcgc gccgaatctc ttcggaacgc tgctggccgc
      601 cggactgacg atgctgatcg cgctgcaggc cttcatcaac atcgcggggg tgatggggct
      661 cattcccctg aaggggctgg cccttccatt cttaagctac ggcggctcgt cgtttgtgat
      721 gagcatgatg acggtgggga ttttgctcaa catctccacg cagaggacgt aaacggcgca
      781 aaaaagacaa cacgcgcggg cgggggaaaa ccggtgagga tagccattgc gggaggagga
      841 acgggcggtc atctgtttcc gggcatagcc gtcgcggaag aatttctgaa gcgcgatcct
      901 caaaaccggg tgatctttat cggaacccgc aggggcatcg agcaccggct tctgggaacg
      961 ctgggatacg agctgaagct cattgatgtg gaagggttga agggaagggg tttgaaggct
     1021 ttgatcaagg gcgtttacgc gataccgaac agcatgatcc agtcggggcg gattctcgcg
     1081 gagtttcggc ccgatgcagt cctcggtgtc ggcgggtatg cctcaggccc cgccgtcctg
     1141 gcggccaggc tgatggggct tccgaccgcc attgcggagc agaacgcgct ggcggggatc
     1201 acgaaccgca ttttggggaa atttgtcgat caaatatttt tgacctatga gcaaagccgg
     1261 ggaagatttg atgtccggaa agtccgcgtc accggcaatc cggtccgggc ggcgtttgtc
     1321 gcgggtctcg ttgctgcccc gacttcgcgc gccttccgtc agattctggt tttcggcggt
     1381 tcgcagggag cggcggccat caacaaaacg gtcgtggcca tgctgccgat gctgcaggga
     1441 atgcgccgcc gggtccgtgt ggtccaccag accggcgagc gcgacctggc ggcggtccgt
     1501 caggcctacg ggaaggccgg cgtggaggct gatgttcaac cgttcatcgt tgatatggcg
     1561 tcggcttacg cgtctgccga tctgattatc tgccgggccg gggcgacatc gctggcggaa
     1621 atcacggcgg cgggaaaagc gtctgtgctg attcccttcc cgtatgcggc caacgaccat
     1681 cagaccgaga atgcgcgggc gatggtccgg gcaggtgccg cggcgatgat cccggagagc
     1741 gggttgaccg ccgggaccct gtatttgctc gtggagactc tgctggaaga cggcgaaaag
     1801 ctgcggggca tggaagagaa atccaaagcg ctgggcaggc ccggcgccgc cgcggaaatc
     1861 gtcgatgcct gcctgcggct gggggcgaaa caaaaaaaca cacaggaaag aggaacatga
     1921 gcaaagacca ggaaaccgga ttcatgcagc ggaaagtcaa gcacatccac tttgtcggca
     1981 tcggcggcat cggaatgagc ggcattgccg aggtgctgct gaatctcgga tacggcatca
     2041 gcggatcgga cgtccagtcc tccgacacga cgaagcgcct gcagcagctg ggtgcggccg
     2101 ttgccgtcgg gcacacggcg gaaaacatcg gcggcgcgga cgtggtggtc acctccaccg
     2161 ccgtaaaggc ggacaacccg gaagttctgg aagcgcaccg caagaatatt ccggtcattc
     2221 cccgggcgga aatgctggcg gagctcctga aaatgaaatt ttccgtggcc gtctccggca
     2281 gccacggcaa gaccacgacc acctcgatga tttcaaccct tctggcggag ggcggactgg
     2341 atcccacgat ggtgatcggc ggcaagctgg ccagcatcgg atccaacgcc cgcctgggcg
     2401 gcggagacat cctcgtcgcg gaggcggacg agagcgacgg ctctttcctg aagctgtcgc
     2461 cgaccatcgc cgtcatcacc aatatcgacc gcgagcatct ggactattat ccgggcattg
     2521 aagaaatcaa ggacgctttt ttaaaatttg ccaacatggt ccccttttac ggctgcacgg
     2581 tgatgtgcgg cgacaacgaa catgtgcgcg ccatttcccc ctcgatcaag cgccgcgtga
     2641 tcacctacgg cataacgcat ccggcggatt acacggcggg cgacatccgg ccttccgggt
     2701 ccaaaacccg atatgccctg tcgtataagg gggagagtct gggcgatgtg gaactggccg
     2761 tgccgggcct cttcaatgtt tacaactcca tggccgccat tgccgtgggg cgcgaactgg
     2821 gcctggaacc ggcgattctc cgcaaaggac tggtggcttt ctccggcgta cagcggcgcc
     2881 tcgaaatcaa gggcgaggcc ggcgggatta ccgtggtgga cgactacggc caccatccga
     2941 cggaaatcat ggcgacgctg gcggcggcgc gccagacgca caagggccgg cttattgtgg
     3001 tgttccagcc gcaccgcttc acgcggacca aggcgctgtt cgacgaattt accagggcgt
     3061 tcacagacgc ggacgtgctg gtgctcaacg atatttatcc ggcgtcggag gcgccgattc
     3121 ccggcgttca ttccgcggcg ctgtgggtgg cgatcaagga aggcggtcat ccctgcgtgg
     3181 aatacatcgc ccagccgcag ggcacggtgg aatttctgct ggccaacgcc aggtccgggg
     3241 ataccgtcct gacgctgggc gcgggcagcg tgtataaaat cggcgaggcg tttctcgaac
     3301 ggctcaacga ggaggctctt ccttcatgac cggtcctgcg aacatagcag cggcgctcaa
     3361 gggcgcaaac atcggccggg tgcggttcaa cgagccgatg tcgcgtcatg cgtcgctgaa
     3421 gctcggcggc cgggtggacg cgctggtgct gcccgaacac gaagcgcagc tcgaagaggc
     3481 cgtgcgcagg ctgcgggaac atcggattcc gtttttgccg gtcgggaatc tcaccaatat
     3541 tctcgtgcgc gacggcgggt accgcggcgc cctgatctcg ctgcgcggtc tggacagggc
     3601 cgtgtgcgcg cccgcaaacg acgggcggtt ttggatcgat gcgcaggcag gcgcgccgct
     3661 gggccggatc gtcgcgcttg ccgccgagag cgaactgacc ggtctggagt tttgcgcggg
     3721 gattcccggc agcgtcggcg gggccgtctg gatgaacgcc ggagcattcg gcgccgaaat
     3781 caaggacgtt ctggttttcg cggatttgat cgacgacggg ggagaaagaa aaaccctccg
     3841 gcgggaggag atcgcttttt cctaccgcaa gtccaatctg cccgcaaatg tcatcatctg
     3901 cggggctcga tttgccctgt cgaaaggccg ttcagagcag atccgggacc gggtggcgga
     3961 aattatgaag tggcgccagg aaaaacatcc gctgcagtat cccagcgcgg ggtcggtttt
     4021 taaaaatctg ccgggaatgc cggcgggccg cctgctggag gagctggggc tgaagggccg
     4081 gcggctgggg gacgtgcaga tttcaaaaaa gcacgccaac ttcatgatca acaaggggca
     4141 gggcacggcg tccgacatgg tgaagctgat ccggctggtg caggaaatgg ttcaaaggga
     4201 aaaaggcctt ttcctggaga cggaaattgt cgtcatcgga gaagaatggt gagccaaaag
     4261 ataaaagtca atctggaagc caaaaaaaac cgtctgcggc ggcgtttgat cggggcgctg
     4321 aacgaatcgc tgggggccct ggccctgctg gcgatggccg tggccgcaag cgccgtgttg
     4381 atttatcttt acaacgcgct tttaagcgcc cccggcctgg agctcaagga aatatccgtg
     4441 cgcggagtga aggagttgac cgaaaaggat atcctggagc tggcgaaaat tcagccccgc
     4501 tgcaatctgc tggcggtcaa caccgacgcc gtggcgcggc ggatcaccgc caatccatgg
     4561 gtcaggaaaa tttatgtcgg ccgggaactg ccgggacggc tggtgctgga cgtgcgcgaa
     4621 cggacgccga ttgcactggt caagcagacg gggaattttt acctcatgga tgctgaaggc
     4681 gtcgtcttta aaaaattaag ccggacggac gatgttgacc tggcgatcct gaccggcgtg
     4741 aatcttggaa aagcgccgtc tcccctgctt gccgaggcgc tgaaactgct ggagattctg
     4801 tccgccgatt cgtacgcatc gctggggacg atttccgaaa ttcatattga cgaggtgttc
     4861 ggcctgtccg tgctgaccga ccggggattg catctgaagc tgggacggga caattttaca
     4921 ggcaaggtgc gccagttgga cgtcgttctg gcggacctgg aaaagcgggg aatgaagaac
     4981 ggccgtttgt ttgtggatct ggccgatatt tccaaggtaa ccgttcagcg caaggacgcg
     5041 ccgggaaagg cgcaggagca gaaaaaaggg ccgcagtaca gaatataggc ggtcacgaag
     5101 aagggtgacg ttatggcgaa aaaaagcaat gttctggtgg gaatcgatat tggaacgacc
     5161 aaaaccatcg cgattgtcgg cgaggtaaaa acgaccggca ttgatatcat cggcatgggg
     5221 attacgcccg ccaaggagct tcgcaaaggc ggcgtggtca acatcgacag caccgtcgag
     5281 gcgatcaaga aggccgtgga agacgccgag catatgtccg gctgccgcat caattccgcc
     5341 tatgtcggaa tcgccggcag ccacgtcaaa ggacagaact cactgggcat tgttgccgtg
     5401 aaaggccggg aagtcggcga agacgatttg cagagggcgc tggaggcctc caaggcgatc
     5461 gccattcccg tcgaccggga aattctgcac acgctgcccc agaactatgt ggtcgatggg
     5521 caggacggca tccgcgatcc ggtgggaatg tcgggcgtgc ggctggaagc caaagtgcat
     5581 atcgtgaccg gcgccgccgc ctcgattcag aacgtggtca agtccgtcaa ccgcgtcggc
     5641 ctggatatcg aagatgtcgt tctggaacag cttgccgcta gcgaagccat tctcagcgcc
     5701 gacgaaaagg atctgggcgt tgcactgatc gacatcggcg gcgcgaccac ggggatggcc
     5761 atctttgcgg aaggaagcat caagcatacg gctacgcttc cggtgggcgg caactttctg
     5821 acctcggaca tcgccacggg cctgcgcacg ccgtttgccg aggcggaaaa aatcaagctg
     5881 aatcacggct gcgccatgac gtcgatgatt ccgaaggaag acgttattga agtgcccagc
     5941 gtgggcgggc gggaagcccg gatggtttca aggcagatcc tgggccggat cgtggagccc
     6001 cggatggatg aaattctgaa catggcgctc aaggaaatcg tgcgctccgg ctacgaggac
     6061 ctgctggcgg cgggcatcgt gctgaccggc ggctcgtcgc tgctgcccgg catcaacgaa
     6121 atggcggagc agatctttga catgccggtg cgcctcgggt gtccagcggg catcggtgga
     6181 ttgagcgatg tagcgaactc gccggccttt gcggtcggcg tcggcctgat tgtttacggc
     6241 agtaaaaaca ttcccggcga ttcagtttac cggaaaacgg ggaatgcatt cggcgaatat
     6301 tttaaaacac tcaaaaaatg gtttctggaa tttttctaaa caaagaaggg ggtgtgc
//
LOCUS       DAOSIM010000115         6353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_11086_length_6353_cov_9.068117, whole genome shotgun sequence.
ACCESSION   DAOSIM010000115 DAOSIM010000000
VERSION     DAOSIM010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11086_length_6353_cov_9.068117"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PLR47_06165"
     CDS             <1..610
                     /locus_tag="PLR47_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOG12565.1"
                     /translation="ADFPAIYAELVKTAETLIYEKGLNHQEIEFTFEGPEKEQLFLLQ
                     TRDMDQTKAKSLRRFRETDLLHASMLGTGIGVSGGALCGRAVYSEKDIQQFRAREPKT
                     PLILIRPDTVPDDVGVLLQVEGLLTAKGGATSHAAVTIPQLGKVGVVGLSKLKVYEME
                     GYATLEGRTIQSGDFIGIDGWSGVLYLGKHDLESTPSATLTL"
     gene            complement(600..1586)
                     /locus_tag="PLR47_06170"
     CDS             complement(600..1586)
                     /locus_tag="PLR47_06170"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOG12566.1"
                     /translation="MPRNGSFEIQCSLEKALAENRNLKLQFDATVRQVHELNSFIRLL
                     LNMSPFGICIVQGGRLIFSNKTFCDICGCSSSELRGIDPLPMVFPEDRDHVQNSVRSS
                     LEEQVDSFFIFRVLTRKDAIKWILGSVNVIHMNGKRAFLGNFVDLTEGRVMQLAYSDP
                     LTGLPNRKLMWDRLEQAMVSAKRRSCRLALLFVDLDKFKDINDAYGHKTGDQLLAQVA
                     DRLRSAVRRENDTLARIGGDEFLILLTDVSQRTDIENFIRLLFEKFRPCLQVGSPPLE
                     VQVGFSVGVALYPEHGESADVLIHHADLAMYQVKKARGEEKFCFFDAQSPTA"
     gene            complement(1795..3066)
                     /locus_tag="PLR47_06175"
     CDS             complement(1795..3066)
                     /locus_tag="PLR47_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HOG12567.1"
                     /translation="MTNSSANESRVPVLGINSLGRIGKLTLWHHISRKYFKEIIVNQG
                     RDIGMGMETIARLIEADATYGLLHRFLYGIKAQPCIQITDDKNGKMLIDGIPVTILRE
                     QRNPLNIPWRQYGADIVVDCSGSFKDPTVPVDDKKGSIRGHLNGGAKVVIHSAPFKIK
                     NKALATPEDTTTLIYGINHTAFNPKKHLLVSAASCTTTGLAHMVKPLLDNEETSTILT
                     ASMSTIHAVTNTQSVLDKLPKAGEKDIRKTRSILNNIILTSTGAAKALAEVIPEVKNI
                     GFMGDSIRVPTNTLSLIVLNATFQARNNDKAAAAGLDTKKLNEIYAKAAGNNPLVRFT
                     MQQNVSTDLIGEDAAVIIEGQFNHTRTAFIPVDLSHIPNLPADLVKSLGEKLLQVPVV
                     HAKIFGWYDNEYGSYTNRMGDLTVYIHKNLQ"
     gene            complement(3333..3659)
                     /gene="hgcB"
                     /locus_tag="PLR47_06180"
     CDS             complement(3333..3659)
                     /gene="hgcB"
                     /locus_tag="PLR47_06180"
                     /inference="COORDINATES: protein motif:HMM:NF040864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation ferredoxin HgcB"
                     /protein_id="HOG12568.1"
                     /translation="MGNLTYLKGVATLQMDEKKCTGCGMCIEVCPHHVFEMNVKNAMI
                     INFDACMECGACRQNCPSGAISVQSGVGCAAAVIGSLLGRNGGECCCGPRTDETGAQK
                     SGGCCS"
     gene            complement(3751..4899)
                     /gene="hgcA"
                     /locus_tag="PLR47_06185"
     CDS             complement(3751..4899)
                     /gene="hgcA"
                     /locus_tag="PLR47_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_264175424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation corrinoid protein HgcA"
                     /protein_id="HOG12569.1"
                     /translation="MDIKNGFEFPVLQEPAGCGCGGPAVPAADDPIARLDQSFVSGSF
                     STPAGDLPKVSSLLTRQDRWGTIKARLGAGRMNYAIDPGLYALGNPDDQSPVLVTANY
                     KMSFDCLREALPNRNAWILVLDTKGINVWCAAGKGTFGTQELLRRIETSGLKKLVSHR
                     KLILPQLGAPGVSAHRVRQSTGFQVYYGPVRASDLPAYLDGKMKKTDQMREITFSMKE
                     RTVLIPIELLSTAKSFLLVSSVLLLLSGLLGPNGFLINIQEIGGFAVAALFCAVLGGA
                     VLNPLLLPCIPGRAFSVKGFIIGLVIALGLIFLRGMDLQSGAGKLEALSWLLIIPAVS
                     AYLAMNFTGCSTYTSLSGVKKEMRLALPLEISAGVFGLAGWIASLVVL"
     gene            complement(4916..5395)
                     /locus_tag="PLR47_06190"
     CDS             complement(4916..5395)
                     /locus_tag="PLR47_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOG12570.1"
                     /translation="MYITTIMKKKEGHPAKLIDVFVGLAQATRCFRQDTAFCGGVTFH
                     QFIILNAAAKNKYLRISDLHSLLAVEKSTTTRLLNPLLEKGLLTREKSASDSRIFGLT
                     LTKSGEETHRQVQLCLEEFFNKVAANLPAGKEENILQAVQIWINAIKNAAGVCNGCQ"
     gene            5668..6117
                     /locus_tag="PLR47_06195"
     CDS             5668..6117
                     /locus_tag="PLR47_06195"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOG12571.1"
                     /translation="MKTYSDAAEEKFIEGYNCAQSVLFSFCEELGLDADTALKISCGF
                     GGGMGRKGEVCGAVTGGVMVLGLKYGRGIKEQREQTYASYVKIRELFDRFSARHSTCL
                     CRELLGGCNLATPEGQAYFKENDCLRLVCRPCVRDAAELVCDLLAEG"
BASE COUNT         1432 a         1662 c         1810 g         1449 t
ORIGIN      
        1 agccgacttt ccggccatct atgccgaact ggtcaaaacc gcggaaacgc tgatctacga
       61 aaagggcctg aaccaccagg aaattgaatt cacttttgaa ggcccggaaa aagaacagct
      121 ttttctgctg cagacgcggg acatggacca gaccaaggcc aaaagcctgc ggcgctttcg
      181 ggagacggac cttctgcacg cctccatgct gggaacggga atcggcgtga gcggcggcgc
      241 cctttgcggc cgggccgttt attccgaaaa ggacattcag cagtttcgcg cccgggagcc
      301 caaaaccccg ctcattctga tccgccccga tacggttccc gacgatgtcg gcgtccttct
      361 gcaggtggag ggactgctca ccgccaaggg cggagcgacc tcccatgccg ccgtgaccat
      421 tccgcaactg ggcaaagtcg gagtggtggg gctgagcaag ctgaaggtct atgaaatgga
      481 aggctatgcg acgctggagg gccgcaccat tcaaagcggg gattttatcg gcatcgacgg
      541 ctggagcggc gttctgtatc tcggaaagca cgacctggaa tcgaccccca gcgcaaccct
      601 cacgctgtag ggctctgcgc gtcaaaaaag cagaactttt cttccccccg ggcttttttc
      661 acctgataca tggccagatc ggcatggtgg atcagaacgt ccgcgctttc gccgtgctcg
      721 ggatagagcg cgacgccgac gctgaaaccg acttgaactt ccaggggagg cgaaccgacc
      781 tgaaggcatg gtctgaattt ttcaaaaaga aggcggatga agttttcgat gtcggttctt
      841 tgtgagacat ccgtcagcag aatcaaaaac tcgtcgccgc cgattcgggc aagcgtgtcg
      901 ttttctcggc ggacggcgct tcgcaggcgg tcggcgacct gcgccagcag ttgatctccg
      961 gttttgtggc cgtaagcatc gttgatgtcc ttgaatttat ccagatcgac aaacagaagg
     1021 gccagtcggc aggaccttcg cttggcggaa accatggcct gctccagccg gtcccacatg
     1081 agcttccggt tcggcagtcc ggtcagggga tcgctgtagg cgagctgcat gacccggcct
     1141 tccgtgaggt ccacgaaatt tcccaggaac gcccgcttcc cgttcatgtg aatgacgttg
     1201 accgatccca gaatccactt gatggcgtcc tttcgggtca gtacgcgaaa aatgaaaaaa
     1261 gagtccacct gctcctccag cgaggaccgg acgctgtttt gcacatggtc ccggtcttcc
     1321 ggaaagacca tcggcaaagg atcgattccc cgcaattcgc tcgaagaaca gccgcaaatg
     1381 tcgcagaagg tcttgttgga aaaaatcagc cggccgccct ggacaatgca gattccgaaa
     1441 ggggacatat tcagcagaag gcggatgaag gaattcagct cgtggacctg ccggacggtc
     1501 gcgtcgaact ggagtttcag gttgcggttt tccgcgagag ctttttccag cgaacactga
     1561 atttcgaatg aaccattccg gggcatgttc cctccgagtg cgcggctgaa tttgttttcg
     1621 gatcgctttt caagcgactg acgcgcgcgt ttttgcgcaa accccgaaca aaagtgaatc
     1681 gccgaggagg agcattcgca tggaggaccc tcgttttccc tttccgggaa aacgagggtg
     1741 tccgggtttg aatcagccgg gagcgggccc aaacccgctc cgattcataa cggcttattg
     1801 aagatttttg tggatataga cggtcaggtc tcccatccgg ttggtgtagc tgccgtattc
     1861 attgtcgtac cagccgaaaa tcttggcatg gaccaccgga acctgcagca atttttcgcc
     1921 gaggctttta acgagatccg ccggcagatt cggaatatga gacaaatcaa ccggtatgaa
     1981 agccgtccgg gtgtgattga attgtccttc gatgatgacc gccgcgtctt cgccgatcag
     2041 atcggtggag acgttctgct gcatggtgaa acggacaagc gggttgttcc cggcggcctt
     2101 cgcgtaaatc tcattgagct ttttggtgtc gaggcccgcc gccgcggctt tgtcgttgtt
     2161 ccgggcctgg aacgtcgcgt tgagcacgat gagcgaaagg gtgttggtcg gaacccggat
     2221 cgaatcgccc atgaagccga tgtttttgac ttccggaata acttcggcca gggccttggc
     2281 ggcgccggtc gaagtcaaaa tgatattgtt cagaatgctg cgtgttttgc ggatgtcttt
     2341 ttcgcccgct ttgggcagct tgtccaggac gctctgcgtg ttggtcacgg cgtgaatcgt
     2401 ggacatggac gccgtcagaa tggtgctggt ttcctcgttg tccagaagcg gtttgaccat
     2461 gtgcgcaagc cccgtcgtgg tgcaggacgc ggcggaaacc agcaggtgct tcttgggatt
     2521 aaacgccgta tggttgatgc cgtagatcag tgtggtggtg tcttccggcg ttgcaagggc
     2581 cttgtttttg atcttgaagg gcgcggaatg aatgaccact ttcgcgccgc cgtttaagtg
     2641 accccggatc gagcctttct tgtcgtccac gggaaccgtc ggatccttga agctgccgct
     2701 gcagtccacg acgatgtccg ccccgtactg acgccaggga atgttcaggg ggttgcgctg
     2761 ttcccgcaga atcgtcaccg gaatgccgtc gatgagcatc ttgccgtttt tatcgtccgt
     2821 aatctgaatg cagggctgcg ctttgatgcc gtacaggaaa cggtgcagca ggccgtaggt
     2881 cgcgtccgcc tcgatcaggc gcgcaatggt ttccatcccc atgccgatgt cccggccctg
     2941 gttgacgata atttccttga agtactttct gctgatgtgg tgccacaggg tcagtttacc
     3001 gattcgcccc agactgttga tgccgagcac cggcaccctg ctttcatttg cactgctgtt
     3061 ggtcatgata tatttcctcc tttacccctc aagacggcgg gtatgatttt gctgttcggc
     3121 ggagttgttc aggagcatag aaccccgatg atggcgatgg aagacaggct ctaaatgaca
     3181 agacgcaaat tcggagtcca taattttaaa gaggtcgttt cgggaagacc ggaactctct
     3241 tataggaaag ggcggccaaa gtcaagttct tataaaaacg ctaataatat tgggatgata
     3301 taatttggag ccgatgcgca atgaaacagg attcacgagc agcagccgcc gcttttttgg
     3361 gctcccgttt catccgtccg cggtccgcag cagcattcgc cgccgtttcg tcccagcagg
     3421 cttccgatga cggcggccgc gcagccgact cccgattgaa cgctgattgc gcccgacggg
     3481 cagttttgac ggcacgcgcc gcattccatg cacgcgtcga aatttataat catcgcgttt
     3541 ttgacattca tttcaaatac atggtgtggg cagacctcga tgcacatgcc gcagccggtg
     3601 catttttttt cgtccatttg aagggttgcg acacctttca gatacgtgag atttcccatt
     3661 tttcctcccg cgatgctgcg ttgcgatccc tttgcacgct gcagaacagg ggcttgcagc
     3721 cccttgttct gatgagccgg cacaggacgc ctacaaaaca accagcgatg caatccagcc
     3781 ggccagtccg aaaacgcccg ccgatatttc cagcggcaag gccagccgca tttctttttt
     3841 tacgccggag agcgatgtat aagtcgaaca gccggtaaaa ttcatggcca gataagccga
     3901 tacggccgga atgatcagca gccaggacaa agcctccagt tttccggcgc ctgattgcag
     3961 atccatgccg cgcaaaaaaa tcagtccgag ggcgatgacc agtccgatga tgaagccctt
     4021 gacggaaaaa gcgcgtccgg gaatgcaggg cagcagaagc ggattaagca ccgccccgcc
     4081 caggacggcg caaaagagcg ccgcgacggc aaaccctcca atctcctgaa tgttgatcag
     4141 aaatccattg ggccccagca agccggacag caaaagcaga accgaagaca ccagcagaaa
     4201 agatttggcc gtggacaaaa gttcgatggg aatcaatact gtccgctctt tcatggagaa
     4261 tgttatttcc ctcatttgat ccgtcttttt cattttgccg tcgagataag cgggcaggtc
     4321 ggatgcgcgg acggggccgt aataaacctg aaagcccgtt gattgcctga cccgatgcgc
     4381 ggacacgccg ggggctccca attgaggcaa aatgagcttt ctgtggctga ccaatttttt
     4441 aagaccgctt gtttctattc tccggagaag ttcctgcgtg ccgaaagtgc cttttcccgc
     4501 ggcacaccat acattgattc ctttagtgtc caggaccaga atccaggcat tcctattcgg
     4561 cagggcttct ctgaggcagt caaagctcat tttgtaattg gcggtgacca gcaccggcga
     4621 ctggtcatcc ggatttccca atgcgtaaag gccgggatcg atggcgtaat tcatgcgtcc
     4681 ggcgcccagg cgggctttga ttgtacccca tcggtcctgc cgtgttaaca gggatgatac
     4741 tttcggcaaa tcacccgcgg gcgtggaaaa cgatcctgag acaaaggact gatccagccg
     4801 cgcaatggga tcgtccgcgg ccggcacggc ggggccgcca cagccgcaac cggccggctc
     4861 ctgaagcacc ggaaattcaa atccattttt tatgtccatc gcttctctca acaaattatt
     4921 gacaaccatt acagacgccg gccgcgtttt tgatcgcatt gatccatatc tgcacggcct
     4981 gcaatatatt ttcctctttt ccggcaggca gattggcggc caccttattg aaaaattcct
     5041 caagacagag ctgaacctgc cggtgggttt cctcgccgct cttcgtgagc gtcagcccga
     5101 agattctgga atcggaggct gatttttccc tggttaaaag tcccttctcg agaagaggat
     5161 tgagcagcct tgttgtcgtg cttttttcca cggccagaag ggagtgcaaa tcggaaatcc
     5221 tgagatattt gtttttggcg gcggcattca ggatgataaa ctgatgaaaa gtcaccccgc
     5281 cgcagaaggc ggtgtcctgc cggaagcatc ttgtcgcctg cgccagaccg acaaacacgt
     5341 ctattaattt ggctggatga ccctctttct ttttcataat ggttgtaata tacaactatg
     5401 tccggctttg tcaagggggg aattgaaaag tgaagagcaa tgagcaatga gtgacgagga
     5461 aaatgggaaa tgggagaagg gctcgaagct catggctgat agaagcaaaa agtgaatggt
     5521 ttatagtgga ttgtggatgg taaaaaaaac aggtgatggg taataggggt ttgaatagga
     5581 aataaaaaat gttattttca tgcacgccgg gtcaatgccc tgcgatcaac ggttcaaaaa
     5641 tgaaaaatga tgggaaaggg attgaatatg aaaacttatt ctgatgcggc cgaggaaaaa
     5701 ttcatcgaag ggtataattg cgcccagtcg gtcctgtttt ccttttgcga ggagctgggg
     5761 ctggatgcgg acaccgcttt gaagatttcc tgcggattcg gcggcggcat gggacggaag
     5821 ggagaggtgt gcggggctgt gaccggcggc gtgatggttc tcgggttgaa atacggcagg
     5881 gggataaaag agcagcggga gcaaacgtat gcctcgtatg tcaaaatacg agagttgttc
     5941 gaccgttttt ccgccaggca ctccacctgt ttgtgccgcg aactgcttgg cggctgcaat
     6001 ctggccacgc cggaaggaca ggcgtatttc aaggaaaatg attgtctccg gcttgtctgc
     6061 cggccctgcg ttcgcgatgc cgccgaactg gtttgcgacc tgctcgccga ggggtaatgt
     6121 tctgcaaaac agccgccgcg ggcgcgattc cgggcgtgac cggggccgcc tcaaccggat
     6181 cattcaagac cggattattg agttgacacg ggaggttcct ttccctatac tcccggccag
     6241 ttaaaatgaa ggagcaggaa aacatatgac gatcaacatc aaagccgaag aacatattat
     6301 gctcattgag atcgaccggc cgaagaaata caacgccatg acccgggaaa tgt
//
LOCUS       DAOSIM010000116         6339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_11111_length_6339_cov_6.869828, whole genome shotgun sequence.
ACCESSION   DAOSIM010000116 DAOSIM010000000
VERSION     DAOSIM010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11111_length_6339_cov_6.869828"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..549)
                     /locus_tag="PLR47_06200"
     CDS             complement(160..549)
                     /locus_tag="PLR47_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOG12572.1"
                     /translation="MIDNTIAAWHKLVEERDAAGLDRILAENVVFHSPVVHTPQAGKP
                     ITTLYLTAALHVLNNDTFHYLREVASGNHAVLEFLTVIDGISINGVDMITWGEDGRIT
                     DFKVMLRPLKAVNLVHKMMGEMLQKIK"
     gene            complement(565..915)
                     /locus_tag="PLR47_06205"
     CDS             complement(565..915)
                     /locus_tag="PLR47_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12573.1"
                     /translation="MSEADGPRITNIKINPDNLYKEESFTDLTFATIRRLTPVKIDGS
                     TDESRQPLFTGMAQLMSPNGPIPVQCLIEGASTLSDAAAKLPDAIEKAVQGMIAEAQE
                     LERQEASKLIVPGQ"
     gene            1528..3549
                     /locus_tag="PLR47_06210"
     CDS             1528..3549
                     /locus_tag="PLR47_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HOG12574.1"
                     /translation="MMTEESRLEKRKLLQDINLMGADYYEKFYQQTFLEWKKFVSGDR
                     DIDRSIIPRQVFDSWVSCAALGLDPFANPDNEVLAGALLQDLLATHGEFIDVSRPFMK
                     NLYRFLEGSGFMVSLLNCKGYVLEIFGDPEEDRLVQTARGYVGACWDLKNTGNNAASA
                     VVLYKKPVQVFGAQHYVRMYHGATGCGSPIFSPEGQLLGCLVLFGRYYRANPHTLGMT
                     VAAANAIENELRIRKALSETRVAYSYQQTVISSIQEALIAVDSTGHVTLMNDNARNMF
                     GFPEDADFDGRRLRDIFKRENEQFFDLIENQETVTDTEVRIFFKNLSGDFTLTCNPIL
                     SSGKNIIGKIIILNEIQRAKSMVTRMMGATANFRFEDIQGQNPLFLMTVEQARMVSRS
                     RSNVLLLGKSGTGKDIFAQAIHNESDRKNGPYVAINCGAIPRDLIASELFGHEEGAFT
                     GSRRGGNQGKFELADGGTLFLDEIAETPLELQTALLRVIEDKSVLRIGGTRVRPVNVR
                     IIFSTNKDLREEVRKGNFREDLYYRANVFSIEMIPLRDRLDDIPLLADCFIRRYAVAM
                     KKSITRVDEKVIETFMSYNWPGNVRELQNTIERMVNFLQTPVLTADLIPEHIRRPDEF
                     FTEVETRPEVDRTMLLRLLNSKMPKNRIAQKMNISRATLYRKIKQFNLQ"
     gene            complement(3536..4564)
                     /locus_tag="PLR47_06215"
     CDS             complement(3536..4564)
                     /locus_tag="PLR47_06215"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOG12575.1"
                     /translation="MWDASKCDYCGDCLVRCRYVDYDRDRAAREIKLLMEGQAADILG
                     SCITCNACVQTCPTGADPADLIYTLQEKTGASPIIAGFKPFIDAVIQGFEEGGAGIIV
                     EGEANKPVLSLDSFGIKQFPKGTMESRMFKGMTVVRGKPYASLVGMCHMGGASLTARY
                     AQKVIEGLSALGKDIVYLHNEGYVLAHVKAKELGIDVPYRYMHLFEYLLDYLKSNAGD
                     ITPLNRKIAYQPNCAARWIPHQDAWLDEIFDRIGVKRVSRKYEGVDALCCGGPALAVN
                     RELAAKIQNDNIWDARDNQAEALVTICPMCDLVLGAPTAKAGLPKIFITDICRMALGE
                     APWPATAN"
     gene            complement(4597..5304)
                     /locus_tag="PLR47_06220"
     CDS             complement(4597..5304)
                     /locus_tag="PLR47_06220"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOG12576.1"
                     /translation="MDLKKVNETLNTYIRPQTFPIAVRLFAAGEALPAKVKIPVRDLG
                     YPIALCQAWALSRRHGMVVAVGKEDQCCIGGHIAMGFMDHPPQGGLGDDSKNHEPGRY
                     AYMVSAPIDRADFEPDLICLFVNSAQAMRLAQAAANGGGGIAPTLPSGMGSCGDIAAR
                     TVKTDLSQFILPSGGTRVYASTQDDELIFTIPKSKIDLMMKALQDTHAIGFRYPVLTD
                     VRHRPNLPPFLEIPKNA"
     gene            complement(5321..6124)
                     /locus_tag="PLR47_06225"
     CDS             complement(5321..6124)
                     /locus_tag="PLR47_06225"
                     /inference="COORDINATES: protein motif:HMM:NF005699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOG12577.1"
                     /translation="MSELVKINIANHIADVRFNRPDKYNALSLGMIDAMADAINRLAK
                     EPGVRVVVISGEGKSFCAGLDVENFAALLSGENKFPNLTERYQNRITNIYQHIGYGWK
                     ELPVPVIAAIHGVALGGGFQIAMGADIRFSTPDAKFSLMEMRWGLIPDMSVTQTIRDF
                     VSMDVAKELIFTGKVIKGEEAARLNLVTHVCEKPHEEAMVLAREIASKNPDAVRAAKK
                     LINEVWHGDSARGLMMESELMMTLMGSPNQLEAMNANFTKQPPKFKDPA"
     gene            complement(6138..>6339)
                     /locus_tag="PLR47_06230"
     CDS             complement(6138..>6339)
                     /locus_tag="PLR47_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12578.1"
                     /translation="EAGTEEIELEVTDTAHKGHVVIPHGFGMIYNGVKYGANVNRLTK
                     NTHRDQFGTPMHRFVPCRVEPA"
BASE COUNT         1360 a         1686 c         1759 g         1534 t
ORIGIN      
        1 cttttcaacc ttttccagca ggtcggccgg atagcctgtt tcatgcgcca ggtctttaat
       61 gctcagtttt ctgctttcgc ggtatgcctt gatgctctga ccgaaggact tcttgtgttc
      121 ttttactttt gccatggatt tcatcctgta aaaaaggcgt cacttgatct tctgcagcat
      181 ttcccccatc attttgtgaa ccaggttgac ggctttcagc ggacggagca tcaccttgaa
      241 gtcggtgatc ctgccgtctt cgccccaagt gatcatatct acgccgttga tggagatgcc
      301 gtcaatgacc gtcagaaatt caagcacggc atggttgccg gaagcgactt cccgaagata
      361 gtgaaacgtg tcgttgttga ggacgtgcag ggccgccgtc aggtacaggg tggtgatcgg
      421 ttttccggcc tgcggcgtat ggaccacggg ggaatgaaaa acgacgttct ccgcaagaat
      481 gcggtccaga ccggctgcgt ctctttcttc aaccagtttg tgccaggcgg cgatggtgtt
      541 gtcgatcatg gctgcacctc tttgttattg gccggggaca atgagtttgg aggcttcctg
      601 ccgctccagt tcctgcgctt cggcgatcat gccttgaacc gctttttcaa tggcgtcggg
      661 cagtttggcc gccgcatcgc tgagcgttga ggcgccttcg atcaggcact gaaccgggat
      721 cggtccgttg ggggacatga gctgagccat tccggtgaaa aggggctggc ggctctcatc
      781 cgtcgaaccg tcgattttga ccggcgtgag gcgccggatc gtggcgaagg tcagatccgt
      841 aaaagactct tctttgtaaa ggttgtcggg attgatctta atgtttgtga ttctgggtcc
      901 gtctgcttcg ctcatgatgg tctcctttta cgttggggtg atgatcatgc cccgaaaaaa
      961 ataaaaggaa actgtgggcg aaacggcctc ctttctttcg gatcactgat ggtttcctac
     1021 actgaaaaag cacgggggtc aaggagggat atgagtgatg agtaacgagt gacgagcgat
     1081 gagtgaatgg tggatggtgg atggtgaata gtggattgtg gatggaaaaa gacaagtgaa
     1141 aagtgatgag tgaaaagaaa cgcaattatc aaaaagatga gcctgaagac gcgttcaagg
     1201 aaggcgaggg agagggtcca gggaaggcat gttggagata cctatttact ccccgaatcc
     1261 ctctccccct tcggtaggat taaaaaaggg ggattttagc ggttagtgaa attaaccgca
     1321 ttgattgtat ttggttcagc caatcattat ttctataaaa tgactagcat tcagtatgcc
     1381 aattcttaaa taattttata ttgttgtttt gttttgcttt ttttaaattt cctctggatt
     1441 tttgtatcaa aaaatgcttt aaggatgtct cactgagaca aaattgagac atttgtgaga
     1501 catccgcatt tctctgtgca gaggtgaatg atgacggaag agtccaggct ggaaaagcga
     1561 aagctgctcc aggatatcaa tctgatgggc gcggattact acgagaaatt ttaccagcag
     1621 acgtttctgg aatggaaaaa atttgtttcg ggtgaccgcg acattgaccg gtccattatt
     1681 ccacggcagg tgtttgattc ctgggtaagc tgtgccgcgc ttggcctgga cccattcgct
     1741 aatccggaca acgaggtatt ggccggagca ttgcttcagg acctgctggc cacccacggg
     1801 gaattcattg acgtcagccg cccgttcatg aaaaacctgt accgttttct ggagggatct
     1861 ggattcatgg tcagtctcct gaactgcaaa gggtatgttc tggagatttt cggagatccc
     1921 gaggaagacc ggctggtgca gacggccaga ggctatgtcg gggcctgctg ggatttgaag
     1981 aacacgggca acaatgccgc gagcgccgtt gtcctttata aaaagcccgt tcaggtgttc
     2041 ggcgcacagc attatgtccg aatgtatcac ggcgcgaccg gatgcggttc gcccattttc
     2101 agtccggaag gacaactgct ggggtgcctt gtgctgttcg gccgctatta ccgcgcgaac
     2161 ccccacacgc tgggcatgac ggtggcggcg gcgaacgcca ttgaaaacga gctgcgcatc
     2221 cgcaaggctc tttccgaaac ccgtgtggcc tacagctatc agcaaacggt catctcatcg
     2281 attcaggagg cgctcattgc ggtggattcc acgggccatg tgacgctgat gaatgacaat
     2341 gcccggaaca tgttcggctt tccggaggat gccgattttg acggacggag gctgcgcgat
     2401 atttttaaac gggaaaacga gcagttcttt gacctgatcg aaaatcagga aaccgtcacc
     2461 gacacggaag tccgcatttt cttcaaaaat ctttcgggag acttcacgct gacgtgcaac
     2521 ccgattctgt cgtccggcaa aaacatcatc ggcaagatta ttatcctcaa tgaaatccag
     2581 cgggccaagt ccatggtgac ccggatgatg ggggcgaccg ccaatttccg ctttgaggac
     2641 attcagggac aaaaccccct tttcctgatg acggtggagc aggccagaat ggtttcccga
     2701 agccgctcca atgttctgct tctgggaaag agcggaaccg gcaaagatat tttcgcccag
     2761 gccattcaca atgagagcga ccggaaaaac ggcccctatg tcgccatcaa ctgcggcgcc
     2821 attccccggg atttgatcgc cagcgaattg ttcggccatg aagaaggggc gttcacgggg
     2881 tcccggcgcg gcggcaacca gggcaaattc gagctggccg acggcggcac gctgtttctc
     2941 gatgaaattg ccgaaacccc gttggagctg caaaccgcgc ttctgagggt gatcgaggac
     3001 aaatcggtgc tgcgcatcgg cggcacacgc gtccggccgg tgaacgtgcg catcattttt
     3061 tcgaccaaca aggacctgcg ggaagaagtc cgcaagggaa acttccgcga ggatctctac
     3121 tatcgcgcca acgttttttc catcgagatg attccgctca gggatcgcct cgacgacatt
     3181 ccgcttttgg ccgactgctt catccgccgc tatgccgtcg caatgaaaaa aagcattacc
     3241 cgtgtggatg aaaaggtgat tgagacgttt atgagctaca actggccggg caatgtacgg
     3301 gaactgcaga acaccatcga acgaatggtc aattttcttc agacgccggt gctgaccgcc
     3361 gatctcattc cggagcacat ccgccgtccg gatgaattct tcacggaggt cgaaacgcgc
     3421 ccggaagtcg atcggacaat gctgttgcgg ctgctgaatt ccaagatgcc caaaaaccgc
     3481 atcgcccaaa agatgaacat ctcccgcgcg accctttacc ggaagataaa acagtttaat
     3541 ttgcagtagc gggccagggc gcctcgccca gggccatgcg gcaaatatcg gtgatgaata
     3601 ttttcggcaa accggctttt gcggtcgggg cgccgagcac gagatcgcac atgggacaga
     3661 tggtcaccag ggcttcggcc tgattgtccc gggcatccca aatgttgtcg ttttgaattt
     3721 tcgccgccag ctcccggttg acggcaagcg ccggtccgcc gcagcacagg gcgtccactc
     3781 cttcgtattt tctcgacacg cgctttacac cgatccgatc gaaaatttcg tccagccacg
     3841 cgtcctgatg gggaatccac cgggcggcgc agtttggctg ataggcgatt ttccggttca
     3901 gcggtgtgat gtcgcctgcg ttgctcttca gatagtcgag caggtattcg aacaggtgca
     3961 tataccggta ggggacgtca atgcccaact ccttcgcttt gacatgggcc agaacgtatc
     4021 cttcgttgtg caggtaaacg atatccttgc cgagcgcgga caggccttca atcacttttt
     4081 gagcatacct tgcggtcaga ctcgcaccac ccatatggca catgccgacc agggaagcgt
     4141 acggctttcc ccgtaccacg gtcatgcctt tgaacattcg gctttccatc gtgcctttcg
     4201 gaaactgctt gatgccgaag gaatccaggg acaaaacagg cttgtttgct tctccttcca
     4261 cgatgatgcc tgcgccgcct tcttcaaacc cctgaatgac ggcatcgata aagggtttaa
     4321 agcctgcgat gatcggactg gccccggttt tctcctgcaa ggtgtagatg agatcggccg
     4381 gatcggcgcc ggtggggcag gtttgaacac aggcgttgca ggtaatgcat gacccaagaa
     4441 tatccgccgc ctggccttcc atgagaagct ttatttcgcg tgcggccctg tccctgtcgt
     4501 aatcgacata gcggcatctg accagacaat cgccgcaata atcgcatttt gaagcatccc
     4561 acatggcttc acctcattaa ttttctttcc cggaggttac gcatttttcg gaatttcgag
     4621 gaagggcggc aggttcggac gatgccgcac atccgtcagg acggggtagc ggaatccgat
     4681 ggcatgcgta tcctgcagcg ccttcatcat aaggtcgatt ttacttttcg ggatcgtgaa
     4741 aatgagttcg tcatcctgcg tggaagcata gaccctggtt ccgcccgagg gaaggataaa
     4801 ctggctcaga tccgttttga ccgtgcgcgc cgcaatgtct ccgcagcttc ccatgccgct
     4861 cggcagcgtc ggagcgatgc cgccgcctcc gttggcggca gcctgcgcca ggcgcatcgc
     4921 ctgggcggag ttgacgaaca gacagatcag gtccggctcg aaatcggccc ggtcgatggg
     4981 cgccgacacc atataggcgt agcggcccgg ctcatgattt tttgaatcgt cgcccagacc
     5041 tccctgcgga ggatggtcca tgaatcccat ggcgatgtgg cctccaatgc agcactggtc
     5101 ttctttgccg acggctacaa ccatgccgtg tcgccgggag agagcccatg cctggcaaag
     5161 cgcgatcgga tagcccagat cccggacggg gattttgact ttggccggaa gcgcttcccc
     5221 ggccgcgaat agcctgacgg caatgggaaa agtctgcggc cggatgtagg tgttcagcgt
     5281 ttcattgact ttcttcaggt ccatgtcttc ctcctttttt tcaagccggg tctttgaatt
     5341 ttggcggctg cttggtaaag tttgcgttca tcgcctccaa ctggttggga cttcccatga
     5401 gcgtcatcat cagctcggat tccatcatca gtcccctggc ggaatctcca tgccagacct
     5461 cattgatcag ctttttggcc gcgcgcacgg catcgggatt tttggacgca atttcccggg
     5521 ccaggaccat agcctcttca tggggctttt cacagacgtg cgtcaccaga ttcaaccggg
     5581 ccgcctcttc gcctttgatg actttgcccg taaagatcag ttccttggcc acgtccatcg
     5641 acacgaaatc acggatggtc tgggtgacgc tcatgtccgg aatcaatccc catctcattt
     5701 ccatcagcga gaacttggca tcgggcgtgc tgaagcgaat gtccgccccc atggcgatct
     5761 ggaaaccgcc gcccagcgct acgccgtgaa tggccgcgat caccggcacg ggaagctctt
     5821 tccacccgta gccgatatgc tgatagatgt tcgttatcct gttctgataa cgctccgtca
     5881 gattgggaaa tttgttttct ccggacaaga gagccgcgaa gttctcgaca tccagcccgg
     5941 cgcaaaagct cttcccttcg ccggaaatga caacaacccg gacgccgggc tcctttgcca
     6001 gccggttgat ggcgtcggcc atggcgtcga tcattcccag actcagcgca ttgtatttgt
     6061 cgggccggtt gaagcggaca tccgctatat ggtttgcaat gttgattttt accagttcac
     6121 tcataatgtc tcctttctca tgcaggttcc acccggcacg gcacaaatcg atgcatcggc
     6181 gtgccgaact ggtcgcggtg cgtgtttttg gtcaggcggt tgacgttggc gccgtacttt
     6241 acgccattgt atatcatccc gaatccatgg ggaatgacca catggccttt gtgcgcggta
     6301 tccgtgactt ccagttcgat ctcttccgtg ccggcctcc
//
LOCUS       DAOSIM010000117         6261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_11297_length_6261_cov_7.910732, whole genome shotgun sequence.
ACCESSION   DAOSIM010000117 DAOSIM010000000
VERSION     DAOSIM010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11297_length_6261_cov_7.910732"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PLR47_06235"
     CDS             complement(<1..319)
                     /locus_tag="PLR47_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12579.1"
                     /translation="MLKDHRPYYVKRFYNRFQKMYAQYFLAPQFEYFGRGLVFMKPWH
                     VEIFGGPVSLGDHSNVIATPDKKVRLTVWPAWKSAGKIEIGRCCLICPGTRIMAATSV
                     TMGD"
     gene            complement(414..1439)
                     /locus_tag="PLR47_06240"
     CDS             complement(414..1439)
                     /locus_tag="PLR47_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12580.1"
                     /translation="MELNISLSEELKPIDVEKVAEFIASLQKENGEIPWSRGGKTDPW
                     DHIESAMGLSVAGWTAEAERAYAWLVSAQLPDGSWWSEIRDGVVTNSTKETHFAAYIA
                     VGVYHHYLITGDRTFLRTMWPSLSRGIQYAVNMQAPDGEIYWARNREGAIDRMALLTG
                     CSSIYMSLKCALAVADLLDIKKPAWQKARERLGETIRSRPDLFNMIKSRFSMDWYYPI
                     LCGAVRGNEAKARIDHLWDKFVVPDWGVRCVSDRPWVTMAETSEFILTLAAMEDHTRA
                     RAIFSWLGDKRYFDGSYWMGVTFPDTVIWPEEKTGWTAAAVLLAWDALNGMTPASKIF
                     AHSSWPE"
     gene            complement(1444..2145)
                     /locus_tag="PLR47_06245"
     CDS             complement(1444..2145)
                     /locus_tag="PLR47_06245"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG12581.1"
                     /translation="MITIDLQELNLKPGFAVLDAGCGTGRHLRALARLPGLKIFGIDR
                     NPADVAEAAKALSEMPDALSREVSVEAADITKMPFANETFDCVICSEVLEHIPEHEAA
                     LKELVRALKPRGTLVVSVPRYFSERICWMISPAYSSDEGGHIRIYKKKNLQKMLAVQG
                     MKCWKINYKHALHAPYWWLKCLVGIKNDNNYFVKLYHRFLVWDMFCKPRAVRVLEEAL
                     NPVIGKSIVFYSKKG"
     gene            complement(2132..3385)
                     /locus_tag="PLR47_06250"
     CDS             complement(2132..3385)
                     /locus_tag="PLR47_06250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG12582.1"
                     /translation="MKKNSNSGPLKICLLTYRGNPTCGGQGVYIKHLSKALADEGHFV
                     DVISGPPYPQLDDRVRLIKMPSLDLYNPDHLFRPERFGDLARPLNLYEFASVCAGGFP
                     EPFSFGERVYAYLKKHRGDYDIVHDNQCLSYGIGRLAQKVMPTIPTIHHPITVDRQEE
                     YKAARTLRQKFRVFRWYSFINMQLKVAKSFSHIITVSAFTRKDIAKEFSLDENKFRVV
                     HNGVNNEYFYPKQNGPRPENSLIVTNSADTPLKGLRYLLEAVDEIRRKRPIRLTVIGQ
                     PKKDGIIENLVARLNLSGTVHFTGRIANEEFADYYAKSTIAVVPSLYEGFGIPAAEAM
                     ACGVPLISAAGGALPEVVGDAGIIVPPADAPALARAIEHLLDSSEERQKYAREGLRRV
                     HSVFSWEKAARESVEVYREAIHDYH"
     gene            complement(3382..4032)
                     /locus_tag="PLR47_06255"
     CDS             complement(3382..4032)
                     /locus_tag="PLR47_06255"
                     /inference="COORDINATES: protein motif:HMM:NF004981.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HOG12583.1"
                     /translation="MIDLHTHTLFSDGALIPAETARRAFVAGYKAIALTDHVDDSNID
                     FVIPRMVRVCGKLSEKGDIFVIPGVELTHVDPSDIASLAAEARKLGAKIVVVHGETLT
                     EPVPPGTNLAAIRAGVDILAHPGLITREETKLAAKKGVYLEITTRRGHSYTNGHVAAM
                     AGKCGADLVLNNDAHAPGDFAGRPMAVNIARGAGLSEKEIVIMFKNSQKIVQRARA"
     gene            4263..6044
                     /locus_tag="PLR47_06260"
     CDS             4263..6044
                     /locus_tag="PLR47_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOG12584.1"
                     /translation="MHKFTLDKPDNLIDWWQESEAKFSDRRLFGTKNKSGVYEWVTFG
                     DVGTRIRNLRAGLAKLDINRGDVVGIIANNRVEWPIVAFAAMYEAELVQIWKYIIADS
                     QIKVLFVSKPEIYEKIKDFPKDIPTLKHIFVIDAEGENSMAALEKKGAAKPVAAQVPT
                     PDDIAELIYTSGTTGNPKGVLLSHGNFTSNSLAGLKMYPELEKNEGRDVVTLAILPWA
                     HSFGQTGELYAVIRLGGSIGLAESPATIVDDIVKVRPTFLVAVPRVFNRIYDGLWAKM
                     NKEGGLAKSLFVMGVDAGKKRRELAARNQSDFLTNLKFKIADKIVFSKIREKLGGRIL
                     GAMTGSAAMNPEIGHFFFDIGLPVYDCYGLTETSPAATMNASYAYRLGSVGRAIEKVR
                     IVIDSSVVEEGATDGEIIVYGPNVMKGYHNRPEDTAASITPDGGFRTGDRGRLDKDGY
                     LFITGRIKEQYKLENGKFCFPVSIEEEICLLPLVQQAVIYGYNRPFNICIVVPDFEAL
                     KSYAKGKGLPTDIKALVERQDVADMYGALITSHLKNKFGGYEIPKKFLLIDEPFTLEN
                     GTLTQTMKLKRKVVLDKFQDRIEALYR"
     gene            complement(6129..>6261)
                     /locus_tag="PLR47_06265"
     CDS             complement(6129..>6261)
                     /locus_tag="PLR47_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOG12585.1"
                     /translation="GTFSVSKRKARKGRNPQTGEAIKIAAKKVPVFKAGKGLKDAVK"
BASE COUNT         1357 a         1826 c         1747 g         1331 t
ORIGIN      
        1 cgtcgcccat cgtgaccgag gtggccgcca tgattctcgt ccccgggcag atcaggcagc
       61 aacggccgat ttcgatcttc cctgcgcttt tccaggccgg ccagacggtc agcctgactt
      121 tcttgtccgg ggtggcaatg acattcgaat gatccccaag ggaaaccggt ccgccgaaga
      181 tttccacgtg ccaaggcttc atgaatacca gtccccggcc gaaatactcg aactgcggcg
      241 ccagaaaata ttgagcgtac attttctgaa accggttgta aaagcgcttg acgtaatagg
      301 gtcggtgatc cttcagcaat gctttcctct tttcaaagtg ccgcggctga caatgaacat
      361 caaaaatccc gccgccggga gtccgcggta ccgttttcgg aaatggatgg ggtttactcc
      421 ggccaggagc tgtgggcgaa tattttcgag gccggcgtca tgccgttgag cgcatcccag
      481 gccaacaaaa ccgcggcggc cgtccagccg gttttttcct ccggccagat caccgtgtcg
      541 ggaaacgtca cgcccatcca gtaggaaccg tcgaaatagc gcttgtcgcc aagccagctg
      601 aagatggccc gcgcccgggt gtggtcttcc atggcggcca gcgtcaggat gaattccgat
      661 gtttcggcca tcgtcaccca gggacggtcg ctgacgcagc gcactcccca gtcgggcacg
      721 acaaacttgt cccacaagtg atcgatgcgg gctttcgcct cgtttcccct gacggctccg
      781 cacaggatgg gatagtacca gtccatcgaa aaacgggatt tgatcatgtt gaaaagatcc
      841 gggcggctgc gtatggtttc tcccagccgc tctctggcct tctgccaggc gggctttttg
      901 atgtccagca ggtccgcgac ggccagcgcg cacttgaggc tcatgtaaat cgagctgcat
      961 ccggtcaaaa gcgccatccg gtcgatggcc ccctcgcggt ttctggccca gtagatttct
     1021 ccatccggcg cctgcatgtt gaccgcgtat tgaatgccgc gtgataaaga cggccacatg
     1081 gttctgagaa aagtcctgtc gcccgtaatc agatagtgat ggtaaacgcc tacggcgata
     1141 taggcggcaa aatgggtttc cttcgttgaa ttggtgacca ctccgtcgcg gatttccgac
     1201 caccagcttc cgtccggcaa ctgggcggaa accagccagg cataggcgcg ctccgcctcg
     1261 gcggtccatc cggcgacgct caggcccatc gcgctttcaa tgtggtccca gggatccgtc
     1321 tttccgcccc gcgaccaggg gatttcaccg ttttccttct gcagggaggc gatgaattcg
     1381 gccacctttt ccacatcaat gggtttgagc tcttcgctca gcgatatatt caattccata
     1441 cggtcagccc tttttcgaat aaaacacgat gctctttccg atgaccggat tcaacgcttc
     1501 ctccagaacc cgcaccgccc ttggcttgca gaacatgtcc caaaccagaa accgatgata
     1561 aagcttcaca aaataattat tgtcgttctt gatgccgacc aggcacttaa gccaccagta
     1621 gggcgcgtgc agcgcatgtt tgtaattgat cttccagcac ttcatcccct gcaccgccag
     1681 catcttttgc agatttttct ttttgtaaat tcggatgtgc ccgccttcgt cgctcgagta
     1741 cgccggagat atcatccagc agatgcgctc ggaaaaatag cgcggaacgc tgacgaccag
     1801 cgtgccccgg ggcttcagcg cgcggaccag ctccttcagc gcggcttcgt gctccggaat
     1861 gtgctccagc acctcggagc agatcacgca gtcgaaagtt tcattcgcaa agggcatttt
     1921 cgtaatatcg gcggcctcca cggaaacctc ccgggaaagc gcgtccggca tctccgagag
     1981 cgctttcgcc gcctcggcca catcggcggg gttgcggtcg attccgaata ttttcaggcc
     2041 cggcagtctc gccagcgccc gcagatgtct gcccgtgccg catccggcat ccagaaccgc
     2101 gaatcccggc ttcagattca gctcctgcaa atcaatggta atcatggatt gcctcgcgat
     2161 aaacctcaac cgattcccgg gcggcctttt cccagctgaa cacggaatga acgcgcctaa
     2221 gcccctcccg cgcgtatttt tgcctttctt cggaagagtc cagcagatgc tcgattgccc
     2281 gcgccagcgc cggcgcatcc gcgggcggaa cgatgatccc ggcgtccccc accacttccg
     2341 gaagggcccc gccggcggcg gaaatcagcg gcacgccgca ggccatcgct tcggccgcgg
     2401 ggatgccgaa tccttcgtac agagacggca cgacggcgat ggtcgatttg gcgtagtaat
     2461 cggcaaattc ctcgttggcg attcgcccgg taaaatgcac ggtgccggag agattcagac
     2521 gggccaccag gttttcaatg atgccgtctt ttttgggctg gccgatcacc gtcagccgga
     2581 tcggccgctt tcggcggatc tcatccaccg cctccagcag gtaccggagc cccttcaggg
     2641 gcgtatccgc gctgttggtc acaatcaggg aattttccgg ccgcggaccg ttttgcttgg
     2701 gataaaagta ttcgttgttg acgccgttgt gcaccacgcg gaatttattt tcatcaaggg
     2761 agaattcctt ggcgatgtcc tttcgggtga aggccgacac ggtgatgatg tgggaaaagc
     2821 ttttggccac cttaagctgc atgttgatga acgagtacca gcggaacacg cggaattttt
     2881 gccgcagcgt cctggccgcc ttgtattctt cctgccggtc cacggtgatc ggatggtgaa
     2941 tcgtggggat cgtcggcatg actttctgcg ccagcctgcc gatgccgtag gacaggcact
     3001 ggttgtcgtg gacaatgtcg taatcgccgc ggtgcttttt cagataagca taaacccgct
     3061 cgccgaagga gaagggctcg ggaaagccgc ccgcgcagac gcttgcaaac tcgtacagat
     3121 tcagcggccg ggccagatcc ccaaagcgct ccggccggaa cagatggtcg ggattgtaaa
     3181 gatccaggct tggcatttta atcaggcgga cgcggtcgtc gagctgcgga tagggaggtc
     3241 cggagatgac atccacaaag tggccttcat cggccagcgc cttgctcaaa tgcttgatat
     3301 acacgccctg cccgccgcag gtgggattgc cacgatacgt gagcagacaa atcttcaacg
     3361 gccctgaatt tgaattcttt ttcacgcacg ggccctttga acgatctttt gggaattttt
     3421 gaacatgatg acgatttctt tttcggataa gcccgcgccg cgggcgatgt ttacggccat
     3481 ggggcggcct gcaaaatcac ccggcgcgtg agcgtcgttg ttgagcacca gatccgcccc
     3541 gcattttccg gccatggcgg ccacgtgtcc gttggtgtag ctgtgccccc ggcgggtggt
     3601 gatttccaga taaacgcctt ttttcgcggc gagcttcgtc tcttcgcgtg taatcagccc
     3661 cgggtgggca agaatatcca ctccggcgcg gatggcggcc agattggtcc cgggaggaac
     3721 cggctcggtg agggtttccc cgtggacgac cactatcttc gcccccagct ttcgggcttc
     3781 ggccgcaagc gaagcgatgt cggagggatc gacatgcgtc agttccacgc cgggtatcac
     3841 gaaaatatcg cctttttccg aaagcttgcc gcaaacccgg accattcgcg gaatcacgaa
     3901 atcgatattg gaatcatcga catggtccgt aagcgcgatc gccttgtacc cggcgacaaa
     3961 ggcccttcgg gccgtctccg ccgggatgag cgcaccgtcg ctgaaaaggg tatgggtatg
     4021 cagatcaatc acgtgccgcc gtccatgagc ttttgagctt ttgcaaaaac tagtggtcac
     4081 ttagcaaatt tgcgggcgtc cgtcaataaa gatgatgaaa tcactgaaaa tgaaacaata
     4141 gttttgattg tgttttgcgg gaaaatgaca tagacagtcc ggcaattgag cggagaacat
     4201 tttcgaatga tggcccggat cgaacggggt ccccgcgaca aaagaacaga aggagatcgt
     4261 ttatgcacaa gtttacactg gacaagcctg acaacctgat cgactggtgg caggaaagcg
     4321 aagcgaagtt ttccgatcga agacttttcg gcaccaagaa caaaagcggc gtttatgaat
     4381 gggtcacgtt tggagacgtg ggaacccgca tccgcaatct cagggcgggt ctggccaagc
     4441 tggacatcaa ccggggagat gtggtcggca ttatcgccaa caaccgcgtg gaatggccca
     4501 ttgtggcttt cgccgccatg tacgaagcgg aactggtgca gatctggaaa tacatcattg
     4561 ccgacagcca gatcaaggtg ctgtttgttt ccaagccgga gatctatgag aaaatcaagg
     4621 atttcccgaa ggatattccc acgctgaagc atattttcgt aatcgacgcc gaaggcgaaa
     4681 attccatggc cgccctggaa aagaaaggcg ccgccaaacc cgtcgccgcg caggttccca
     4741 caccggacga catcgccgaa ctcatctaca cgtcgggcac caccggcaac cccaagggcg
     4801 ttcttctgtc ccacggcaac ttcaccagca actccctggc cggactgaaa atgtatcccg
     4861 aactggaaaa aaacgaagga agagacgtcg tgaccctggc cattctgccc tgggcccatt
     4921 ctttcggcca gaccggcgaa ctctacgcgg tcatccgtct gggcggctcc ataggcctgg
     4981 cggaaagccc cgccacgatt gtcgatgaca tcgtcaaagt gaggcccacc tttctggtcg
     5041 ccgtgccccg cgttttcaac cgcatctacg acggcctctg ggccaaaatg aacaaagaag
     5101 gggggctggc caaatcgctg tttgtcatgg gcgtggacgc cggaaagaaa aggcgggaac
     5161 tggccgcccg gaaccaatcc gattttctca ccaatctcaa attcaagatc gcggacaaaa
     5221 tcgtcttttc gaaaatccgg gaaaagctgg gcggccggat actgggcgcc atgaccggca
     5281 gcgccgcgat gaatccggaa atcggtcatt tcttctttga catcggtctg ccggtttacg
     5341 actgctacgg tctcacggag acctcgcctg ccgcaacgat gaacgcctcc tacgcctacc
     5401 gcctggggag cgtcggacgc gccatcgaga aggtcaggat cgtgattgat tcttcggtgg
     5461 tggaggaagg cgccacggac ggcgaaatca tcgtttacgg ccccaacgtc atgaagggct
     5521 atcacaaccg gcccgaggac acggccgctt cgataacgcc cgacggcggt ttccgcacgg
     5581 gcgaccgcgg ccgcctggac aaggacggct acctcttcat cacgggccgc atcaaggagc
     5641 agtataagct ggaaaacgga aagttctgct tccccgtctc catcgaggaa gaaatctgcc
     5701 tgttgcccct ggttcagcag gcggtcattt acggatacaa ccgccccttc aacatctgca
     5761 tcgtcgttcc cgattttgaa gcgctgaaaa gctacgcgaa gggaaaaggc ctgccgacgg
     5821 acatcaaagc gctggtggaa aggcaggacg tggcggacat gtacggcgcg ctgatcacga
     5881 gccacctcaa aaacaagttc ggcggctacg aaatccccaa aaagtttttg ctgattgacg
     5941 aaccgttcac gctggaaaac ggcacgctga cccagaccat gaaactcaag cgcaaagtgg
     6001 tcctggataa atttcaggac cggattgaag cgctgtaccg ataaacggat cgtcgcccgt
     6061 cacaccgcaa taaaaaagcc ggctcctttt atgagagccg gctttttttt gatgatgaca
     6121 aatgaaattt atttaaccgc gtccttcaaa ccctttccgg ccttgaaaac cggaaccttc
     6181 ttggcggcga tcttgatggc ttcgccggtc tgggggtttc tgcctttgcg ggcctttctc
     6241 ttgctgacag agaaggtgcc g
//
LOCUS       DAOSIM010000118         6119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_11625_length_6119_cov_7.019954, whole genome shotgun sequence.
ACCESSION   DAOSIM010000118 DAOSIM010000000
VERSION     DAOSIM010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11625_length_6119_cov_7.019954"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>746
                     /locus_tag="PLR47_06270"
     CDS             <1..>746
                     /locus_tag="PLR47_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG12586.1"
                     /translation="KNKAAIIFEGNDPNDWKVYTYYDMYREVNKFANVLKSLGVKKGD
                     RVTIFLPMIAELSIVMLACARIGAVHAIVFGGFSAEALRDRINNSGSAVVITCDGTYR
                     GAKAVPQKDTCDKAVAQCPGVKTMVVVKRTGTQVNMKEGRDIWYEELMAKADRYCKPE
                     EMDAEDPLFILYTSGSTGQPKGVVHTQAGYLLYASMTQQLAFDVREEDTYWCTADIGW
                     VTGHSYIVYGPLCNGHSSILFEGVPNYPDF"
     assembly_gap    747..756
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <757..1690
                     /locus_tag="PLR47_06275"
     CDS             <757..1690
                     /locus_tag="PLR47_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG12587.1"
                     /translation="RFWAVVEKYNVNNFYTAPTAIRSIAKEGDKWVDAHDISSLRVLG
                     SVGEPLNPEAWWWYYNKIGAGRCTISDTWWQTETGGHMILPLPGAIDIKPAKAMVPFF
                     GVVPVLFDEEGKEIKGPGKGALCIKNSWPGMARGLWNDTEGRFVENYFVQFPGTYFSG
                     DGAERDADGDYKITGRVDDVINVSGHRLGTAEVEAALTSHADVAEAAVVGFPHDIKGQ
                     GIFAYVTLNNNVEKTDELKKALVAHVRKVVGPIASPDQIQWADGLPKTRSGKIMRRVL
                     RNVAAKTFGDFGDTSTLADPHVVDDLVENRKKLG"
     gene            complement(1912..4020)
                     /locus_tag="PLR47_06280"
     CDS             complement(1912..4020)
                     /locus_tag="PLR47_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOG12588.1"
                     /translation="MEKLFYPQSIAIMGLSSRPDNIPRLVLENLIRWGYLGRIFGVNP
                     RSTDPHVDGIKMYRDIGELPAVPDLVFVLIPARFVPDAVDACGKAGVRWMAIPSGGFN
                     ELGEEGRKLAEQVVRRARQYGIRFVGPNGVTVANTANGLCLPFVPSFSPPKGGLSIIT
                     QSGGVGLMLWNLMTDENVGMAKFASIGNKLDLDEVDFLEYFGRDPDTRVIGMYLESIP
                     RGDRLIEAASRISKPVIVLKSNTTDAGKKVAMSHTAAISNNEAILDAAFERAGITRIH
                     EFNDFISMAKVFDLPPMRGKRIMVMSPAGGFTVMMADLCEESGFAFADPGPAFYDELK
                     NSSNAGVIHFSNPLDMGDIYDAGAYSNIFFSVMHNENVDGAVYVTQWPEMPRGEDVFY
                     KMFHTDLSKEVTGTILSSGKPLGICLFGLSATISRIKRNLDIPIFNNAGEMIRAFKKQ
                     HDYYARKADGSPVHALPDRIDIRAAKTWIADHPGVKGEEALELLRHGGIAAAESVVAG
                     NANEAAAAAERIGYPVVMKIVSPDAVHKSDAGGVITGIETAEAVRSAFDRIRRNLDRC
                     KKDARFEGIRVMKQAGEGYDMFIGGQRDASFGPVVSFGYGGIYIEVFKDVAHVLCPAG
                     SREIEEKIRKLKSCKILQGARGKGAGDIPGYAAAIERVSHLLHQFPQIRELDVNPLRV
                     LADGSGVVALDARIRIVPNP"
     gene            complement(4077..4586)
                     /locus_tag="PLR47_06285"
     CDS             complement(4077..4586)
                     /locus_tag="PLR47_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG12589.1"
                     /translation="MQYETRMALKNGKTVPVRPVLPTDESFIVDLLNKLSSDSIYLRF
                     LRPVKALPKELLFQLTHLDYQRNFALAAVIREDEKDAIIGVARYGYDPQEQLTDFAIV
                     VRDDWQHLGLGKQMLSKIFAIGRKQGIGRFVSIVDSTNIAMKRILRELDYPVNYSYEK
                     GFTQVEVLV"
     gene            complement(4660..>6119)
                     /locus_tag="PLR47_06290"
     CDS             complement(4660..>6119)
                     /locus_tag="PLR47_06290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG12590.1"
                     /translation="MVQSIGLLEGLKKGNVGMVAQAGVYAAGILTGLSNVLDFGIVAT
                     IGNKMDINETDILEYLSDDDHISTIVMYMEDIRSGKRFVDVASRAAVRKPVIVLKSGR
                     TEAGKKAVSSHTASLAGNDEVNSAAFKQCGVIRARDNEHLFALARAFSKQPVPKGNGT
                     LVITYTGSLGVAATDMLYLSGMRLAELEPYFQKRLKNALPEFANAGNPIDCSFSMTPE
                     QVKNLIEIGVESSDVQSFIVVIQGEILGSFVDVMKSIDYKEKPAVCVVACKEFMIHDV
                     VKMEQAGFPVYSTAEMAAEVLGQMHAYGVRRQSAFTDSIARHLTKDTLSVDNVPVQLR
                     LINPKDIGLWTDFVNSCSPKSLWLRFLSPFSATPERAQRFCNVNPEDEVALVAEAKTG
                     DQRKFLGIARLLKNKRCEGEAEYAIIISDPWQNKSLGITLSEQCIELAKKEGYKTIRA
                     ETLQENYAMIRIFRRCAFQFDGKDENMVSMTLNLS"
BASE COUNT         1253 a         1761 c         1771 g         1324 t
ORIGIN      
        1 agaaaaacaa agccgcgatc attttcgaag gcaacgatcc caacgactgg aaagtttaca
       61 cctattacga catgtatcgc gaagtcaaca aattcgcgaa tgttctgaaa agcctgggcg
      121 tcaaaaaggg cgaccgagtc accatcttcc tgcccatgat cgccgagctg tcgatcgtca
      181 tgctggcctg cgcccgcatc ggcgccgttc atgccatcgt tttcggcgga ttctccgcgg
      241 aagcgttaag agaccgcatc aacaattccg gttccgcagt ggttattacc tgcgacggga
      301 cctaccgcgg cgccaaggcc gttccccaga aagacacctg cgacaaggcc gtcgctcagt
      361 gccccggcgt gaaaaccatg gtggttgtga agcgcaccgg aacacaggtc aacatgaagg
      421 aaggccgcga tatctggtac gaagaattaa tggccaaagc agaccgctac tgcaaacccg
      481 aagaaatgga cgcggaagat ccgctgttca ttctttacac ctccggttcg accgggcagc
      541 ccaagggcgt cgttcacacc caggccggtt acctgctgta tgcttcgatg actcagcagc
      601 tggcgtttga cgtgcgtgaa gaagacacct actggtgcac cgccgacatc ggctgggtca
      661 ccggtcacag ctacattgtc tatggtcctc tgtgcaacgg ccattccagc attctgtttg
      721 aaggcgtgcc caattatccg gatttcnnnn nnnnnnccgg ttctgggccg tcgtcgaaaa
      781 atacaatgtc aacaatttct ataccgctcc tacagccatc cgctccatcg ccaaagaagg
      841 cgacaagtgg gtcgatgcgc acgatatttc ctcgctgcgc gttctgggtt cggtcggcga
      901 gccgctcaac cccgaagcct ggtggtggta ctacaataag atcggcgccg gccgctgcac
      961 gatttccgat acctggtggc agaccgaaac cggcggacac atgatcctgc ctctgcccgg
     1021 cgcgattgac atcaaaccgg ccaaagccat ggtgccgttc ttcggcgtgg ttccggttct
     1081 gtttgacgaa gaaggcaaag aaatcaaagg tcccggcaaa ggcgctctgt gtattaaaaa
     1141 ttcctggccc ggcatggccc gcggtttgtg gaatgacacg gaaggccggt tcgtcgaaaa
     1201 ttactttgtg cagttccccg gcacgtactt ctccggcgac ggcgcggaac gcgacgccga
     1261 cggcgactac aaaatcaccg gccgtgttga cgacgtcatc aatgtttccg gtcaccggct
     1321 gggcaccgcg gaagtcgaag cggcgctcac ctcccatgcg gatgtcgcgg aagcggccgt
     1381 tgtcggattc ccgcatgaca tcaaaggtca gggcatcttc gcttacgtca cgctgaacaa
     1441 caacgtggaa aagacggatg aactcaaaaa ggccctggtc gcccatgtcc gcaaggtcgt
     1501 cggtccgatt gcatcgcccg accagattca atgggcggat ggtctgccca aaaccagatc
     1561 cggcaagatt atgcgccgcg ttctgagaaa cgtcgccgcg aaaacgttcg gtgacttcgg
     1621 cgacacgtca accctggcgg atccgcatgt ggttgatgac ctggtcgaaa acaggaagaa
     1681 actcggctaa agaccccttg tgcggaacgg ctgccgttcc gcatgtaagt ccctccttaa
     1741 aaagccgttc cccggtttcg gggaacggct ttttgctttg gccggatcag atcggaccgg
     1801 attttccgta ttcccttgcc ctcctgatgc ccgggctctt tccgggcata gatccgcctt
     1861 tccttcaggc aaaccgccct gcgtcaggac gcttccgttc gggcataacc attatgggtt
     1921 gggaacgatt cgtatcctcg catccagcgc gacgacgccg gagccgtcgg ccaaaacccg
     1981 cagcgggttg acatccagtt cgcggatctg aggaaattga tggagcagat gggagacccg
     2041 ttcaatggcg gccgcgtagc cgggaatatc cccggcgcct ttcccccggg cgccctgcag
     2101 gatcttgcag gatttgagtt tccggatttt ttcttcaatc tcccggctgc cggcggggca
     2161 caggacatgg gcgacgtcct taaacacttc gatgtagatg ccgccgtatc cgaaagaaac
     2221 aaccggtcca aaggaagcat cgcgctgccc gccgatgaac atgtcgtatc cttcgccggc
     2281 ctgtttcatg acgcggattc cctcgaaccg ggcgtctttt ttgcagcgat caagattccg
     2341 gcgtatgcgg tcaaaggcgc ttcggacggc ttcggcagtt tcaatccccg tgatgacgcc
     2401 gcctgcgtct gatttgtgga cggcgtcggg cgagacgatt ttcatcacca ccgggtagcc
     2461 gatgcgctcg gccgcggcgg cggcctcgtt cgcgtttccg gccacgacgg attcggcagc
     2521 ggcgatgccg ccgtggcgga gcagctccag ggcttcctcg cctttgacgc ctggatgatc
     2581 cgcgatccag gtcttcgcgg cccggatgtc gattcggtct ggcaaagcat ggacgggcga
     2641 accatccgct tttctggcgt aatagtcatg ctgtttttta aaggcgcgga tcatttcgcc
     2701 ggcgttattg aagatgggaa tgtcgagatt ccgcttgatc cgggaaatcg tcgccgaaag
     2761 tccgaacagg cagatgccca gcggctttcc cgaagacaga atggttcccg ttacttcctt
     2821 ggagaggtcc gtatggaaca ttttgtaaaa aacgtcctct ccccgcggca tttccggcca
     2881 ttgcgtgacg tagacggctc cgtcgacgtt ttcgttgtgc atgacggaga agaaaatatt
     2941 ggaatacgcg ccggcatcat agatgtcgcc catgtcgagg gggttggaaa aatggatgac
     3001 cccggcgttg gaagaatttt tgagttcatc atagaaagcc gggccgggat ccgcgaacgc
     3061 gaagccggac tcctcgcaga gatccgccat catgaccgtg aatccgcccg cgggggacat
     3121 gaccatgatc cgcttgccgc gcatgggagg cagatcaaac accttggcca tcgagatgaa
     3181 atcgttgaac tcgtgaatcc gggtaatgcc cgccctttca aaggccgcat cgagaatggc
     3241 ttcgttgttg gaaatcgccg ccgtatggct catggcgact tttttgccgg catcggtcgt
     3301 gttggatttc aggacgatca cgggtttgct gatccgggac gcggcctcga tcaggcggtc
     3361 tccccggggg atgctttcca ggtacatgcc gatgaccctg gtgtcgggat cgcggccgaa
     3421 atattccagg aagtccacct cgtccaggtc cagtttgttg ccgatgctcg caaatttcgc
     3481 catcccgacg ttttcatcgg tcatgaggtt ccagagcatc aaacccacgc cgccgctctg
     3541 ggtgatgatg gacagtcctc ccttgggcgg actgaaggac ggcacaaaag gcaggcacag
     3601 gccgttggcg gtgttggcta ccgtgacgcc gttgggtccg acgaaccgga tgccgtattg
     3661 acgggcccgg cgcactacct gctcggcaag tttccggcct tcttccccca gttcattgaa
     3721 tccgccggaa gggatggcca tccatcgaac gccggctttt ccgcaggcat cgacggcatc
     3781 ggggacaaac cgcgcgggaa tcaaaacaaa aacaagatcc ggcaccgccg gcagttctcc
     3841 gatgtccctg tacatcttga tgccgtccac gtgcggatcg gtggatcggg ggttgacgcc
     3901 gaagatgcgc ccgagatacc cccagcggat gaggttttcc agaacaaggc ggggaatgtt
     3961 gtcgggcctc gacgacaggc ccatgatggc gatggattgc gggtaaaaaa gtttttccat
     4021 aatattttcc gtttgacggt ttccgaagtg atggcgtccg ggcggatgag gttctcctat
     4081 accagcacct ccacctgggt gaatcccttc tcgtaggaat agttcaccgg atagtcgagt
     4141 tcccggagga tccgcttcat cgcgatgttg gttgaatcga cgatggacac aaaccgacca
     4201 atcccctgct ttcggccgat cgcaaatatt tttgacagca tctgtttgcc gagacctaaa
     4261 tgctgccagt cgtcgcgcac cacgatcgcg aagtccgtga gctgctcctg gggatcgtag
     4321 ccataccgcg ccactccgat gatggcgtct ttttcatcct cgcggatgac ggccgccagc
     4381 gcgaagtttc tttgataatc gagatgcgtg agttggaaga gcagctcctt cggcagagcc
     4441 ttgacgggcc tcaaaaaacg cagatagatc gaatcggacg aaagcttgtt cagcaaatcc
     4501 acgatgaaag attcatccgt cggcaaaaca ggcctgaccg gcactgtttt cccgtttttc
     4561 agggccatac gggtttcgta ttgcaaaaga tcgctccggt tcattgttga tggcattttc
     4621 attacccaac cggcggcagc gcgcggtctg ccgccttgct cacgacagat tcagcgtcat
     4681 cgacaccatg ttttcatcct tgccgtcgaa ctggaaggcg cagcggcgga agatgcggat
     4741 catggcgtaa ttttcctgaa gcgtttccgc ccggatggtt ttgtatcctt cctttttggc
     4801 cagctcgatg cactgctccg acagggtaat ccccagggat ttgttctgcc acggatcgga
     4861 aatgatgatg gcgtattccg cttctccctc gcagcgcttg tttttgagga ggcgcgcgat
     4921 gccgaggaac ttgcgctgat cccccgtttt cgcctcggcg acaagggcca cttcatcttc
     4981 cggattgacg ttgcaaaaac gttgcgcccg ctccggcgtg gcggaaaaag gcgacagaaa
     5041 gcgaagccag agggatttgg gggagcagct gttgacaaaa tccgtccaca ggccgatgtc
     5101 ttttggattg atcagcctca gttgaacggg gacattgtcc acggaaagag tgtccttggt
     5161 cagatgacgg gcgatggaat ccgtgaaggc gctctggcgc cgcacgccgt aggcatgcat
     5221 ctgccccagc acttccgccg ccatttccgc ggtggaatac acagggaagc cggcctgctc
     5281 catcttgacc acgtcgtgaa tcatgaactc cttgcaggcg accacgcaga ccgccggttt
     5341 ttccttgtag tcgatggact tcatgacgtc cacgaacgat cccaggattt cgccctgaat
     5401 gacgacgatg aagctctgca cgtcgctcga ttccacgccg atttcgatca ggtttttcac
     5461 ctgctcgggc gtcatggaaa aagagcagtc gatgggattg cccgcattgg cgaattccgg
     5521 cagggcgttt ttcaggcgct tttgaaaata cggctccagt tcggccaggc gcatgccgga
     5581 cagatacagc atgtcggtgg cggccacgcc gagcgacccg gtgtaggtga tgaccagcgt
     5641 gccgttgcct ttcggaacgg gctgcttgga gaaggcgcgc gcgagcgcga acaaatgctc
     5701 gttgtcccgc gcccggatga cgccgcactg cttgaaggcg gcgctgttga cctcgtcgtt
     5761 gccggccagc gacgcggtgt gcgaggagac agccttcttg cccgcctccg tccgtccgga
     5821 tttaagaacg atgacgggtt tgcggaccgc cgcccgcgag gcgacatcga cgaagcgctt
     5881 gccgctgcgg atgtcctcca tgtacatgac gatggtggag atgtggtcgt cgtcgctcag
     5941 gtattcgaga atgtcggttt cgttgatgtc catcttgttg ccgatggtcg cgacaatgcc
     6001 gaaatccagc acgttgctta agcccgtgag gatgcccgcg gcgtagacgc cggcctgggc
     6061 caccatgccg acattgcctt ttttcagccc ttccagaagg ccgatggact gtaccatgt
//
LOCUS       DAOSIM010000119         6073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_11741_length_6073_cov_7.348455, whole genome shotgun sequence.
ACCESSION   DAOSIM010000119 DAOSIM010000000
VERSION     DAOSIM010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11741_length_6073_cov_7.348455"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="PLR47_06295"
     CDS             complement(<1..165)
                     /locus_tag="PLR47_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOG12591.1"
                     /translation="MNILIFGPNGSGKGTQGAIVQKKFGVPHIETGVIFRQNISKKTP
                     LGEKAKAFIDR"
     gene            complement(289..1728)
                     /locus_tag="PLR47_06300"
     CDS             complement(289..1728)
                     /locus_tag="PLR47_06300"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOG12592.1"
                     /translation="MRKLAQNQYFYLLVLVLMLSGIRNAWSAFTIDDEKKVGKEIYEK
                     LDQENYLSHDKKLNAYVSEIGGRILAHSNKASFPYTFTVFNSSAINAFATPGGYVYIN
                     KGLISVVETEAQLAGVMAHEIAHANSRHVASIIEKNKKLNIAMLAGIIAGALLGGGGD
                     AAAAAAMFSVAGAATLSLKYQREHEEEADRLGIGYLAQAGYYPAAMVEFLKIMKQYEF
                     LSKTIPSYFLTHPGTDDRIFYLESLLQTEYRRSGGSANIVGNIVRMQALITPDAGDLK
                     KRRGQLTESLAKDPRNVDLLYALAVTEDQLGHTGEALENLKKALSLAPRDEDVLKSAG
                     LIYLKAGDARQARFYLQKASAINPENDQTTLALGKAYFAEGDYAGALNYFLKLQHQTI
                     EGEDINYHIAMSYGRLNQMADSHYYFGLYFKKENKTQSALFHFRKAQELTPAGTPKAA
                     AIAAAMNELSPGKRSKPDSKPPARETAPK"
     gene            1837..2520
                     /gene="cmk"
                     /locus_tag="PLR47_06305"
     CDS             1837..2520
                     /gene="cmk"
                     /locus_tag="PLR47_06305"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOG12593.1"
                     /translation="MTKKVITIDGPAGAGKSTVSKALADRLKYLYLDTGALYRALAFK
                     ALKKNIDVNHPGELAGLCSVTDVVLKNINGKMTVWVDGEDVGEKIRAEEVGLAASTIS
                     AYAVVREKLLSLQREAGADGGIVAEGRDMGSVVFPDADFKFYLDADLEERIRRRCGEL
                     ACRTGGAEPSSVSRDMRARDEQDTNRKIAPLAAAPDAVIIDSTSLTIPQVVEKILEHL
                     SGASVSGKP"
     gene            2623..>4131
                     /locus_tag="PLR47_06310"
     CDS             2623..>4131
                     /locus_tag="PLR47_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOG12594.1"
                     /translation="MVNNNNTNSTSEKEVKTDSSVSGAMNQSTQSREEDLGFKELYEQ
                     SLNQLQYGDIACGKVVQITADTVMVDVGWKTEGFIPIRELKDAQGNVTVSVGDDIEVF
                     IDKRDSEGNLVLSREKAAKLKVWDEIKTVSENNQRIKGTVVQKVKGGLSVDIGIIAFL
                     PGSQIDIRPVKDLDQFVGQALEFNILKYDRKRNNVVLSRRAIVAAEREEEKKVTLDNL
                     AEGGIVEGVIKNITDYGIFIDLGGIDGLLHVTDISWGRIAKPADLFQKGQKITVKVLS
                     FDREKERVSLGLKQLHENPWENISEKYPVGVLVQGKVVNLTDYGVFIELEPGVEGLVH
                     ISEMYWTREIKHPSKVMNVGDVVNVVVLEVNPEAKRVSLSLKQTTANPWEKLKEKYPV
                     GTMVKGQVRNITNFGVFVGIEDGIDGLVHVSDISWKHRVTHPSEYFKKGQEVEAVVLN
                     IDVDNEKFSLGIKQIEKNPWEELPRKYAPGSVVTGKITNFTDFGIFVEIEDGI"
     assembly_gap    4132..4141
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4142..4347
                     /locus_tag="PLR47_06315"
     CDS             <4142..4347
                     /locus_tag="PLR47_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOG12595.1"
                     /translation="LYNVGDKVSAVVKSIDIKTKKIRLSIKDMEAPAPTPSSNPYLNN
                     RENVGSTLAQALADVKIGGQSKE"
     gene            4352..5245
                     /gene="sppA"
                     /locus_tag="PLR47_06320"
     CDS             4352..5245
                     /gene="sppA"
                     /locus_tag="PLR47_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOG12596.1"
                     /translation="MRKHPVVLGFVLLIALGAVVYFMSYRAGVQSGGGMKGFSMNDKI
                     GVVPVEGVIADSLEIAENLEAFGNDSSIVAVVVRIDSPGGGVAASQEIYDAVVALKKK
                     KKVVASMGSIAASGGLLIACAADKIVANPGTITGSISAVMQFANVEELMKKIGVKSSV
                     VKSGPYKDIGSPMREMTPEEKVIIQELIDDIYNQFIDVIVRDRKLTRPQVIAIGDGRV
                     FSGRKAKEYGLVDQLGDLAAAAKLAGTLAGSHGRYDLVYPPEKSPSILDYVLESAAGR
                     FARSVRQKAETWGGAGYLYIP"
     gene            5267..5551
                     /locus_tag="PLR47_06325"
     CDS             5267..5551
                     /locus_tag="PLR47_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOG12597.1"
                     /translation="MTKNELIKKVRARCRTCSTQDIALAVKTILEAMAGALKENDRIE
                     IRGFGTFTVRQRKSRVARNPKSGARVSLKDRRAPFFKTGKDLRARVENSR"
     gene            5548..>6073
                     /locus_tag="PLR47_06330"
     CDS             5548..>6073
                     /locus_tag="PLR47_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOG12598.1"
                     /translation="MTYKNIIVEKKKGYAIIRFNRAHEMNALSKEMRLEFNDALLDVE
                     NDTEMRALVITGGEYVFSAGMDIKEMSLLSNVEIDDYFESMKRYLKKISTFPKPVIAA
                     VGGIALGGGFNIVTVCDLIIASESAIFCHPELKFGFSPLFYPLSQIVGVNKAKEIAML
                     GEPIGAREAERIGLV"
BASE COUNT         1411 a         1533 c         1803 g         1316 t
ORIGIN      
        1 gcggtcgata aacgccttgg ccttttctcc cagcggggtt tttttggaga tgttctggcg
       61 gaagatcacg ccggtttcaa tgtggggaac tccgaatttc ttctgcacaa tcgcgccctg
      121 cgtgcctttg ccgctgccgt tgggtccaaa tattaaaatg ttcactgttg catactcctt
      181 tcataaaaat agattgaacc agccgcctta taatgtattg tccctttttt cgcaacttta
      241 aagattgacc ggcggtttgg gcttaagatt ttgatttttt atgcggtttt attttggtgc
      301 ggtttcccgg gcaggcggtt tggaatcggg cttgctgcgc ttgccgggac tcagttcatt
      361 catggcggcg gcaatggcgg cggctttcgg cgttcccgcc ggtgtcaatt cctgcgcttt
      421 gcggaaatgg aaaagcgcgc tttgggtttt attctctttt ttgaaataca aaccgaagta
      481 atagtgagaa tcggccattt gattgagccg gccgtaggac atggcgatat ggtagttgat
      541 gtcctcgcct tcaatggttt gatgctgcag cttcaaaaaa taattcaggg cgccggcgta
      601 gtcgccctcg gcaaaatacg ccttgcccag ggccagtgtc gtttgatcgt tttccggatt
      661 gatcgcggac gctttttgca gatagaagcg ggcctgccgg gcatcgcccg ccttcaaata
      721 tatcaggccg gcgcttttca ggacatcctc gtcacgcggc gcgagcgaca gggctttttt
      781 caggttttcc agcgcttcgc cggtgtgtcc gagctggtct tccgtgacgg ccagcgcata
      841 tagcaggtcc acgttccggg ggtcttttgc cagcgattcg gtcaattggc cgcgcctttt
      901 tttcaggtcg cccgcatccg gcgtaatcaa ggcctgcatg cgcacgatgt tcccgacgat
      961 attggccgat ccgccgctcc ggcgatactc ggtttgcagc agactttcca ggtaaaagat
     1021 gcggtcatcc gtgcccgggt gcgtcaggaa ataggaggga atggttttgg ataaaaattc
     1081 atactgcttc atgattttga ggaattccac catcgccgcg ggatagtagc cggcttgcgc
     1141 cagatagccg atgcccagcc ggtccgcctc ttcttcatgc tcccgttgat atttgaggct
     1201 cagcgttgcc gcgcccgcga ccgaaaacat cgccgccgcc gcggcggcgt ctccgccccc
     1261 gcccaagagc gcgccggcga tgattccggc cagcatggcg atgttgagct tcttgttttt
     1321 ctcaatgatg ctggcgacgt gccgcgaatt cgcgtgcgcg atttcatggg ccatgacgcc
     1381 cgcaagctgc gcctcagtct ccacgacgct gatcagtccc ttgttgatgt aaacatagcc
     1441 gcctggggtg gcgaaggcgt tgatggccga actgttgaag acggtgaacg tgtagggaaa
     1501 cgatgccttg ttgctgtggg ccagaatccg gccgccgatt tccgacacgt aggcgttgag
     1561 ctttttgtca tgcgaaagat agttttcctg gtccagcttt tcataaattt ctttgcccac
     1621 ttttttttcg tcatcaatgg tgaacgcgga ccaggcgttc cggattccag aaagcatcag
     1681 aaccaggacc agcaaataaa aatattgatt ttgagctaat ttgcgcatag gccatcggac
     1741 aaccgttttt tgtgggaaga atcctacccg ctttgttcgc aataggcaag ctttccttgc
     1801 ggggacgggc gcgggcgggg aaaaggagta aatgaaatga cgaagaaagt gattacgatt
     1861 gacgggccgg cgggagcggg gaaaagcacg gtgagcaaag cgctggccga ccggcttaag
     1921 tatctgtatc tggatacggg ggcgctgtac cgggcgctgg ccttcaaggc gctcaaaaaa
     1981 aacattgatg tgaatcaccc cggcgagctg gccggattgt gttcggtcac cgatgtcgtc
     2041 ctgaaaaata tcaacggcaa aatgacggtt tgggtggacg gcgaggacgt gggcgaaaaa
     2101 atccgcgcgg aggaagtcgg ccttgccgcc tcgactattt ccgcttatgc ggttgtccgc
     2161 gaaaaactgc tttccctgca gcgggaagca ggcgcggacg gaggcattgt ggcggaagga
     2221 agagacatgg gatcggtggt tttcccggac gcggatttca agttttatct ggacgccgat
     2281 ctggaggaaa ggattcgaag gcgatgcggc gaactggcct gcagaaccgg cggggcggaa
     2341 ccatcttccg tgtccaggga catgcgggcc agagacgaac aggataccaa cagaaaaatc
     2401 gcgccgttag ccgcggcccc ggacgccgtc atcattgact cgacgtcttt gacgattccc
     2461 caggtcgtcg aaaaaattct tgagcatctg tccggcgcct ccgtttcggg aaagccgtaa
     2521 acggggaagc tttgccgcct gatttatctt gatatttgat tttcagtatg ttaggcaact
     2581 tagattgaaa aagtcttaaa aaaattaggg ggtatcaggt taatggttaa caataacaac
     2641 acaaactcaa caagtgaaaa ggaggttaag acggactctt ccgtttccgg cgcaatgaac
     2701 cagtccacgc agtccaggga agaggatctg ggctttaagg agctctacga gcagagcctg
     2761 aatcaactgc aatacggcga catcgcctgc ggcaaggttg tgcagatcac cgcggacacg
     2821 gtgatggtgg atgtcggctg gaagacggaa gggtttatcc ccatcaggga actgaaagac
     2881 gcccagggaa acgtaacggt ttccgtcggc gacgacatag aagtgtttat cgacaagagg
     2941 gactccgaag gaaatctggt tctgtcccgg gagaaggcgg ccaagctgaa agtctgggat
     3001 gaaatcaaaa cggtcagcga aaacaatcag cgaatcaagg ggaccgtcgt gcagaaggtc
     3061 aagggcgggc tttccgttga tatcggcatc atcgcgtttc tgcccggttc gcaaatcgat
     3121 atccgtccgg tcaaggacct ggaccagttt gtcggccagg cgctggagtt caacatcctg
     3181 aagtatgacc gcaagcgcaa caacgtcgtt ctttcccgaa gggcgattgt ggccgccgag
     3241 cgggaagagg aaaagaaagt cacgctggac aaccttgccg agggcggcat tgtcgagggc
     3301 gtcattaaaa acatcacgga ctacggaatt ttcatcgacc tgggcggcat cgacggcctg
     3361 ctccatgtga cggatatctc ctgggggaga atcgccaagc ctgcggacct tttccagaag
     3421 ggccagaaga ttacggtgaa ggttctttcc tttgaccgcg agaaggaaag ggtttccctg
     3481 ggtctcaagc agcttcatga aaacccctgg gagaatattt ccgaaaagta tccggtgggc
     3541 gtcctggtgc agggcaaggt ggtcaacctg acggactacg gcgtgtttat cgagctggag
     3601 cccggcgtgg aaggactggt ccacatttcg gaaatgtact ggacgcggga aatcaagcat
     3661 ccgtccaaag tcatgaacgt cggggacgtc gtgaacgtgg tggtgctgga agtcaacccg
     3721 gaggcgaaga gagtttccct cagcctgaag cagacgacgg ccaatccctg ggagaagctg
     3781 aaagaaaaat acccggtcgg aacgatggtg aagggtcagg tgcgcaatat cacgaatttc
     3841 ggcgtgtttg tcggcatcga ggacggcatc gacggcctgg tccacgtgtc ggatatttcc
     3901 tggaagcacc gggtcaccca tccctccgaa tatttcaaga agggccagga ggttgaagcg
     3961 gtcgtcctga atatcgacgt ggacaacgag aaattctccc tgggcatcaa gcagattgaa
     4021 aagaacccct gggaggagct tcccaggaaa tatgcgcccg gcagcgtggt gacgggcaaa
     4081 atcaccaact ttaccgactt cgggattttt gtggaaatcg aagacggcat cnnnnnnnnn
     4141 naattgtaca acgtcggcga caaggtgtcc gcggtggtca agagcatcga catcaagacc
     4201 aagaaaatcc ggctgagcat caaggacatg gaagcgccgg cgccgacgcc ttcgtccaat
     4261 ccgtacctga acaaccgtga aaatgtcgga tcaacgctgg cacaggccct ggccgacgta
     4321 aaaatcggcg gccagtcgaa ggaataaatg catgagaaaa catcccgtcg ttctggggtt
     4381 cgttttgctg attgcgctgg gagcggtcgt gtatttcatg tcgtaccggg cgggggttca
     4441 gtccggcggc ggcatgaagg gtttctcgat gaacgataaa atcggcgtgg tgcccgtgga
     4501 aggcgtcatt gcggattcgc tcgaaatcgc cgaaaacctg gaagcgtttg gaaacgacag
     4561 ttcgattgtc gcggtcgtcg tgcgcatcga ttcgcccggc gggggtgttg ccgcctcgca
     4621 ggaaatctac gacgccgtcg tcgcgcttaa aaaaaagaaa aaagtggtag cgtccatggg
     4681 gtcgatcgcc gcgtccggcg gtctgctgat cgcctgcgcc gccgataaaa tcgtggccaa
     4741 tccgggaacc atcacaggca gcatatcggc cgtcatgcag tttgccaacg tggaagaact
     4801 catgaaaaaa atcggcgtga agtcctccgt ggtcaaaagc ggtccgtaca aagacatcgg
     4861 atctcccatg cgggaaatga cgcccgaaga aaaagtcatc atccaggaac tcatcgacga
     4921 catctacaat cagttcatcg acgtgattgt ccgggatcgc aagcttaccc gtccgcaggt
     4981 gatcgccatc ggggacggcc gtgttttttc ggggcgcaaa gccaaggaat acggtttggt
     5041 ggatcaactg ggcgatctcg ccgccgcggc gaaactggcc ggtactctgg ccggaagcca
     5101 tggccggtac gacctggtgt atcctccgga aaagagccct tccattcttg attacgtcct
     5161 ggagagcgcc gccggccggt tcgcgcggtc cgtacggcaa aaggctgaaa cctggggagg
     5221 ggccggttat ttgtacattc catgaccgga ggctctccgt gccgccatga ccaaaaatga
     5281 actcataaaa aaagtgcggg caagatgcag gacctgctcg acccaggaca tcgccctggc
     5341 cgtaaaaaca atcctggagg ccatggccgg agcgctgaag gaaaacgacc ggatcgagat
     5401 tcggggcttc ggcacgttta cggtgcgcca aagaaagtcc cgggtggccc gcaatcccaa
     5461 atcgggcgcc cgcgtatcgc tgaaagacag aagagcccct ttttttaaaa cgggaaaaga
     5521 tctccgtgca agggtggaga attcgagatg acgtacaaaa atattatcgt tgagaagaaa
     5581 aagggctatg cgatcattcg attcaaccgc gcccacgaga tgaacgccct gtccaaagaa
     5641 atgaggctgg aattcaacga cgctctgctg gacgtggaaa acgacacgga gatgagggcg
     5701 ctggtcatca ccggaggcga atatgttttc tccgccggca tggacatcaa ggaaatgtcg
     5761 cttctgtcca acgtggagat cgacgactac ttcgagtcca tgaagcggta tctgaaaaaa
     5821 atttctacgt tccccaagcc cgtgattgcc gcggtcggcg gaatcgccct gggcggcggg
     5881 ttcaacatcg tgaccgtctg cgacctgatc atcgcttcgg agagcgccat tttctgtcac
     5941 ccggagctga agttcggatt cagccctctt ttttatcccc tcagccagat tgtcggggtg
     6001 aacaaggcca aggaaatcgc gatgctgggg gagcccatcg gcgcgagaga agcggaaaga
     6061 atcggtctgg tca
//
LOCUS       DAOSIM010000120         6038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_11836_length_6038_cov_5.423533, whole genome shotgun sequence.
ACCESSION   DAOSIM010000120 DAOSIM010000000
VERSION     DAOSIM010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11836_length_6038_cov_5.423533"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="PLR47_06335"
     CDS             <1..850
                     /locus_tag="PLR47_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG12599.1"
                     /translation="NEFDPGIIRSAIHADKPAAASTYPETLAQRPPNLCPGCPHRGLF
                     YALKKSKAYVHGDIGCYTLSYLKPLEGLHSCICMGASIGMAHGMSKALKEKGKGKVVG
                     VIGDSTFLHSGITPLMNMAYNNSDALIIILDNSTTAMTGMQEHPGTGYTLMGKEAKKV
                     NLNILISALGIENIRVIDPNNVKEARSAIKDELASSGPSVIIAQSPCVLFKRANQKSK
                     PPLKVDPDACEGCRSCLELNCPPIAWKKGVTKKGSKRKGIAVIDETLCNGCGLCAQVC
                     RFGAIS"
     gene            867..1448
                     /locus_tag="PLR47_06340"
     CDS             867..1448
                     /locus_tag="PLR47_06340"
                     /inference="COORDINATES: protein motif:HMM:NF005322.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOG12600.1"
                     /translation="MKEDRVKSILFAGVGGQGIIRASDILCEVMMEAGYDVKKSEVHG
                     MAQRGGCVTSHVRYGRKVYSPLAEPGTVETLVSFEKMEALRYLKDLSPNASIILNTEQ
                     INPPAVNTGDADYPFDIIEYLKMHYPQVMAIEALLLAQRAGNLKAANVVLLGALSSLM
                     DIPHAIWESVIRKSFPQKLVKLNLEAFQMGIPA"
     gene            1488..2444
                     /locus_tag="PLR47_06345"
     CDS             1488..2444
                     /locus_tag="PLR47_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOG12601.1"
                     /translation="MAEDYYKVLGVEKKATPEEIKKAYRKLAVKWHPDKNPNNKAAAE
                     EKFKKISEAYAVLSDAEKRQNYDNFGSADQFRQQYSQEDIFRGFDLDEILRSFGFGGP
                     RGGGRTTFRTGTRRGGYTEYDDPLAGIFGQMGGGGGQYANMPQKGQDAEYNLSISLEE
                     SVLGADKKISLQMEKGAENINVKIPPGISSGKKLRLPGKGLPGYNGGQNGDLYLNINV
                     LSHPIFARDGNDLYIEKTIKFSQAALGTTIDVPTIDGTMKRLKIAPGTQNNTKIRMKG
                     FGVPVLKESRNGDQFVKVNVEVPKKLTDKQARLIEQLAADGM"
     gene            complement(2494..2886)
                     /locus_tag="PLR47_06350"
     CDS             complement(2494..2886)
                     /locus_tag="PLR47_06350"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOG12602.1"
                     /translation="MDDFTKELWKKSPFIKLLGLEFEKLEQGRTSTRLERRENLLNKH
                     SAVHGGVIYAIADISMGVAVYTTLKTGEETSTIEIKINYLKPAKCNLLICEARVLKKG
                     KNLAVVEAEIRSGESDLIAKALGTFMLT"
     gene            complement(2927..3685)
                     /locus_tag="PLR47_06355"
     CDS             complement(2927..3685)
                     /locus_tag="PLR47_06355"
                     /inference="COORDINATES: protein motif:HMM:NF003323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HOG12603.1"
                     /translation="MRFWGRGVRFIADMIHEERTYRSLINKDNLISYRVKISQSDLWI
                     SSDSNLASVAEKSLLKHRFSLESYIDRHPEFQTALLPYPQDFLAPGIVREMIARSAVC
                     GVGPMASVAGAIAEFVGNDLSGLTDHLIIENGGDIFLLSKSSLTIGIFAGESTLSYKV
                     KIIVKPEETPLGVCTSSATVGPSLSFGKADAVCVLSSSPTLADAAASAIGNRVNTRYD
                     IRNAMNCGMKIPGVKGIVIIFGNEMGAMGEVELA"
     gene            complement(3648..>6038)
                     /gene="dnaE"
                     /locus_tag="PLR47_06360"
     CDS             complement(3648..>6038)
                     /gene="dnaE"
                     /locus_tag="PLR47_06360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOG12604.1"
                     /translation="KHAKHNAVPVGPGRGSAAGSLVAYAIRITDIDPLRYGLLFERFL
                     NPDRISMPDIDIDFCQEGRGKIIKYVTEKYGHDKVTQISTFGKMMAKAVIKDVGRALG
                     ISYAETDRIAKLVPNVLNITLAEAFKMEPRFDEERKKNPQIDKLLSMSLVLEGLNRHS
                     SIHAAGIVISDAPLVERVPLFKPKEDLVSQYSMKDIDKVGLIKFDFLGLKTLTVINDA
                     LRFIRNTHNIDIDIRNLPLDDKKTYQLLSKGDTDGVFQLESSGMKDLLINFKPDTIED
                     VIALIAAYRPGPMQMIPDFIARKHGKQKITYELPQLEPILKETYGIILYQEQVMQIAN
                     LIGDYTMAQADTLRKVMGKKLTSDMEKERPKFLAGAKKLKINENKAKIIWEQMEKFAA
                     YGFNKSHSAAYAMITYQTAYLKAHYPVAFMAALLTSEKDNRDKIIRHMSNCKEMGINI
                     LPPDINESQKDFSISGGNIRFGLAAVKNVGEAAIESVMEIRMEGKFTSFMDFLCRIDL
                     RKVNRRVLESLIKCGSFDSLHFTRRQLMECLDSAMEEAQRKQKELQSNQASIFDELAP
                     ARTSGTGGNGSFSIPELPEWDRKELLSIEKDTVGFYISGHPLQGYAGKLHLVANTDSS
                     SLNTKRDKETITVAGIISSLSERLTKKKEVMCNVVLEDLQGSVNIIFWADIYRKFSDL
                     LHADEPVVIQGMVDIGDESNKIIALDVIPLAKALENPYKQVRFMVKTDKISPEGISAL
                     CETIRKYDGKYEGYIHLLNGKCETIVRLGDRTKLNICEKLRREADAILGEGSTVYC"
BASE COUNT         1478 a         1521 c         1542 g         1497 t
ORIGIN      
        1 gaatgaattt gaccccggca tcattcgaag cgcgatccat gccgataagc cggccgccgc
       61 ttcgacttat ccggaaaccc tggcgcaacg gccgccgaac ctctgtcccg gatgcccgca
      121 ccggggattg ttttacgcgc tgaaaaaatc caaagcttac gtccacggcg atatcggctg
      181 ctacacgctg tcctatctga agcccctgga agggctgcat tcctgcatct gcatgggcgc
      241 cagcatcggc atggcccacg ggatgagcaa ggcccttaag gaaaaaggaa aggggaaggt
      301 ggtcggcgtg atcggcgatt ccacctttct tcactccggc atcacgcccc tcatgaacat
      361 ggcctacaac aacagcgatg ccctgatcat cattctggac aacagcacga cggccatgac
      421 cggcatgcag gagcatccgg gaacgggcta caccctgatg gggaaggaag ccaagaaagt
      481 caacctgaat attctgatat cagccctggg aattgaaaat attcgagtga tcgatccgaa
      541 caacgtgaag gaagcccgca gtgcgatcaa ggatgaactg gcgagctccg gcccttccgt
      601 tattatcgcc cagagcccct gcgttttgtt caaaagggca aaccagaagt cgaagccgcc
      661 gctgaaggtg gatccggacg catgcgaagg ctgcaggtcc tgcctggagc tgaactgtcc
      721 gccgatcgcc tggaaaaaag gcgtcacgaa gaagggctcc aagcgaaagg gaatcgcggt
      781 gattgacgag acgctgtgca acgggtgcgg gctttgcgca caggtctgca gattcggcgc
      841 gatcagctga agaaaaggaa agttttatga aagaagacag ggtaaaaagc attctgtttg
      901 ccggagtggg cgggcagggc atcattcggg ccagcgatat cctctgcgaa gtcatgatgg
      961 aagccggcta cgatgtcaag aaaagcgaag tccacggcat ggcgcagcgc ggcggctgtg
     1021 tcaccagcca cgttcgttac ggcagaaagg tttattcgcc cctcgcggag ccgggaaccg
     1081 ttgaaacgct ggtttccttt gagaaaatgg aagccctgcg ctatctgaaa gacttaagcc
     1141 cgaatgcttc catcattctg aataccgagc agatcaatcc cccggccgtc aatacggggg
     1201 atgctgatta tccatttgat atcattgaat atctaaaaat gcattaccct caggtaatgg
     1261 cgattgaggc acttttgctc gcccagaggg ccggcaatct gaaagcggcc aatgtcgttc
     1321 ttttgggagc tctttcctca ctgatggaca ttccgcatgc aatctgggaa agcgttattc
     1381 ggaaatcctt tccgcaaaaa cttgtaaaat tgaatcttga agcttttcaa atgggaattc
     1441 ctgcttaaat taccgccata agaaaagaaa aataagagga aatttgcatg gctgaagatt
     1501 actataaggt tctgggcgtt gaaaaaaagg ccacgccgga ggaaatcaaa aaagcctacc
     1561 gaaagctggc cgtcaagtgg catccggata aaaatcccaa caacaaagcg gcagccgagg
     1621 agaaattcaa aaaaatcagc gaggcttacg ctgttttaag tgatgcggaa aagaggcaga
     1681 attacgacaa tttcggctcg gctgatcagt ttcgccagca atattcacaa gaagacatct
     1741 tccgcgggtt cgacctcgac gagattttgc gcagtttcgg cttcggcggg cccaggggag
     1801 gggggcgaac aaccttccgc acgggaacga gacggggcgg ctatacggaa tatgacgacc
     1861 cgctggccgg cattttcggt caaatgggcg ggggcggcgg ccagtatgcc aatatgccgc
     1921 aaaaaggcca ggatgccgaa tataacctat caatatccct tgaagaatca gttttgggcg
     1981 ccgacaagaa aatttcgctt cagatggaaa agggcgctga aaacattaat gtcaaaatac
     2041 ccccgggaat cagctccgga aagaaactga ggcttcccgg aaaaggcctg ccgggttaca
     2101 acggcgggca aaacggtgat ctctacctga atatcaatgt tttgtcccat ccgatcttcg
     2161 cccgcgacgg caatgacctg tacatcgaaa aaaccatcaa gttttcccag gccgctctgg
     2221 ggacaaccat cgacgttccc acgatcgacg gaaccatgaa aagactgaag atcgcgcccg
     2281 gaacgcaaaa caataccaag attcgaatga agggatttgg agtgcctgtc ctgaaggaat
     2341 cacgcaatgg cgatcagttc gtcaaagtca acgttgaggt tccgaaaaaa ctcaccgata
     2401 agcaggctcg attgattgaa caactggcgg cggatgggat gtagcagccc gcccgccgca
     2461 ttgccgcatt ttctttatgt cctataagat atcttatgtt aacatgaatg tgccgagagc
     2521 ctttgcgatc agatcgcttt ctcccgacct tatctccgcc tccacgacgg ctaaattttt
     2581 tcctttttta agaactcgcg cttcgcaaat taaaagattg catttcgccg gctttagata
     2641 gttgatcttg atttcaatcg tggaagtttc ttcacctgtt ttcaatgtgg tgtaaacagc
     2701 cacgcccatg ctgatatcgg ctatggcata gataacgcct ccgtgaacgg cgctgtgttt
     2761 gttgagcagg ttttctctcc tttcaagacg ggtggaagtg cgcccctgtt ccagcttttc
     2821 aaattcaagc ccaagcagtt tgataaacgg gcttttcttc catagttcct ttgtaaaatc
     2881 atccatatag tgatttcctt ccttcttcct gagatattgt tgtatttcag gcaagctcga
     2941 cctcccccat tgcgcccatt tcatttccaa agataatgac aatgcccttc acgcccggta
     3001 ttttcattcc gcaattcatg gcatttcgaa tatcgtatcg ggtatttacg cgattgccga
     3061 ttgcggaggc cgctgcatcc gccagcgtcg gggaactgga aagaacgcag accgcgtcgg
     3121 cttttccgaa gctgagagac ggcccgaccg ttgcggatga tgtacagaca cccaaaggcg
     3181 tttcttcagg ttttacaatt attttaacct tataacttaa agtagattct ccggcaaaga
     3241 ttccgatggt caggctgctt ttggaaagaa gaaaaatatc ccctccgttt tcaatgatga
     3301 gatgatccgt caaaccggaa agatcgtttc ccacaaattc cgcgattgcg cctgcaacgg
     3361 aggccattgg ccctaccccg cagacggctg atcgcgcaat catctccctg acgattcctg
     3421 gcgccagaaa gtcctgtgga taaggaagca gcgccgtttg aaattccgga tgcctgtcga
     3481 tataggattc aagggaaaat cgatgcttca gaagagattt ttcggccact gaggccaggt
     3541 tggaatcaga gcttatccac aggtcgcttt gagaaatttt aacacgatat gaaatcagat
     3601 tatctttatt aattaatgat ctataagttc tttcctcatg tatcatatca gcaataaacc
     3661 gtactcccct cccccagaat cgcatccgcc tcccgtcgga gtttctcaca gatgtttagt
     3721 tttgtgcggt ccccgagacg gacgatggtt tcgcatttcc cgttcaacag gtgaatatag
     3781 ccttcgtatt tcccatcgta ttttcgaatc gtttcacaaa gggcggaaat tccctccggg
     3841 gatattttgt cggttttgac cataaaacga acctgtttat agggattttc cagagccttg
     3901 gccaggggaa tcacatccag agcgatgatt ttattagatt catctccgat atcgaccatc
     3961 ccctgaataa caaccggctc gtcggcatgg agcaaatcgg agaatttcct gtagatgtcc
     4021 gcccagaaga tgatattgac ggaaccctgc aggtcttcaa gaacaacatt gcacatcact
     4081 tcttttttct tggtcagcct ctcagagagc gagctgatga tccccgcgac ggtgatggtt
     4141 tctttgtctc ttttggtgtt cagcgatgaa gaatccgtgt tggcaaccag gtgcaacttg
     4201 ccggcatagc cctgcaatgg atgcccggaa atataaaaac cgacggtgtc tttttcgatg
     4261 gagagcagtt ccttgcggtc ccattcgggc agctcgggaa tcgagaaaga cccgttgccg
     4321 cctgtcccgg atgttctggc gggagcgagc tcgtcgaaga tgcttgcctg attgctttgc
     4381 aattccttct gcttgcgctg cgcctcctcc atggccgaat ccaggcattc catgagctga
     4441 cggcgggtga agtgcaggga atcgaaagat ccgcacttga tcagactttc caggaccctt
     4501 cggttgactt tgcgcagatc gatgcggcag agaaaatcca tgaaggatgt aaattttcct
     4561 tccatgcgga tttccattac ggattcaatc gccgcttccc cgacattttt tacggcggca
     4621 aggccgaaac ggatgttgcc tcccgaaatg ctgaaatcct tttgggattc attgatgtcc
     4681 ggcggcagaa tattgatgcc catttccttg cagttggaca tgtgccggat aatcttgtcc
     4741 cggttgtctt tttcgctggt caaaagcgcc gccatgaagg cgacgggata atgggccttc
     4801 agataggccg tctggtaggt gatcatcgcg taggccgcgc tgtgggactt gttgaatcca
     4861 tacgcggcaa atttttccat ctgttcccag atgattttag ccttgttttc atttattttg
     4921 agcttttttg ctcccgccag gaattttggc ctttcctttt ccatgtcgga ggtcagcttt
     4981 ttacccatca ccttgcgcaa tgtgtccgcc tgggccatgg tgtaatcgcc gatgaggttg
     5041 gcaatctgca tcacctgctc ctgatacagt atgatgccgt aggtttcctt cagaatcggc
     5101 tccagctgcg gcaactcata ggtgattttc tgcttgccgt gcttgcgtgc gatgaaatcg
     5161 ggaatcatct gcatcggccc cggccggtag gcggcaatca gcgcgatgac gtcttcgatc
     5221 gtgtcgggct tgaaattgat caacaggtct ttcatgccgg aactttcgag ctggaagacc
     5281 ccgtctgtat cccctttact caaaagctga tatgttttct tgtcatcgag cggcaggttc
     5341 ctgatatcaa tatctatatt gtgagtgttc ctgatgaagc gcaacgcatc attgattacg
     5401 gtgagcgttt tgaggcccag aaagtcaaat ttgatgagac cgaccttgtc gatgtctttc
     5461 atggaatatt gggaaacaag gtcttcttta ggcttgaaaa gcggcactct ttcaaccagc
     5521 ggtgcgtcag agatgacaat accggcggca tgaatcgagg agtggcggtt gagcccttca
     5581 agaaccagcg acattgaaag aagcttgtcg atctgcgggt tctttttgcg ctcttcgtca
     5641 aagcgtggct ccattttaaa ggcttcagca agtgttatat tcaacacatt cggcaccagt
     5701 ttggcgatac ggtccgtttc ggcataggaa atgcccaggg cgcggccgac atccttgatg
     5761 accgccttgg ccatcatttt tccaaaagtg ctgatctggg tgactttgtc atgaccgtat
     5821 ttttccgtta cgtacttgat gattttgccc ctgccctcct ggcagaagtc aatatcaata
     5881 tcgggcatgc tgatacggtc cggattcagg aagcgctcaa acagcagtcc gtagcgcaaa
     5941 gggtcgatat ccgtaattcg tattgcgtag gccaccagag acccagcggc ggagccgcgg
     6001 cccggcccca cgggaacggc attgtgtttg gcgtgctt
//
LOCUS       DAOSIM010000121         5992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_11944_length_5992_cov_9.363989, whole genome shotgun sequence.
ACCESSION   DAOSIM010000121 DAOSIM010000000
VERSION     DAOSIM010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11944_length_5992_cov_9.363989"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PLR47_06365"
     CDS             complement(<1..621)
                     /locus_tag="PLR47_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HOG12605.1"
                     /translation="MCRLFAVTSSQPQSPVMAINALNVMKEGHDGSGVGLFLTDLGGE
                     FEKFKNEPILSGIFSNEGIKALDHFMIDQDFMVKYKLSLRPTKPAPSGTPKRDNYVIR
                     VYEYPAEWENLSREEIEYRLMMVQLQLRQIGEKDQSMLIFSFWPDVIMIKEVGDPLAV
                     AEYLGLDRRDLTARVILCQGRQNTNYSINIYACHPFFIQGMATMTNG"
     gene            complement(843..2381)
                     /locus_tag="PLR47_06370"
     CDS             complement(843..2381)
                     /locus_tag="PLR47_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOG12606.1"
                     /translation="MSIHSYEELAALHAISKILAQPMDLRDQLEKILHEMSLRLGMQR
                     GMISLLDHEEKEAWLDIAHDVNIDGMEVTYKPGEGITGQVAETGRPMAVANLGQETHF
                     LDRTGARRHLNRAELSFLCVPIIYDERVVGVLSADKVAKQVEDLEKELAMMGSVAELM
                     AKAVHIRALEEENRRLRKIVGQQHAPSTDIIGHSKAIQEVFGLVAQVSDSNTTVLITG
                     ETGTGKELIARAIHNNSPRKSGPLVQVNCAAIPDTLIESELFGHEKGAFTGALQTRRG
                     RFEEANGGTIFLDEVGELSAAAQVKLLRVLQEKRFQPLGSSRVVNVNVRIIAATNRIL
                     EQDILADRFRADLFYRLNVFPVYLPPLRERGSDVILLADHFLLKYSKEMGKNVKKIST
                     AAIEAFLSHKWPGNVRELENCIERAVLLTKTDTVDCLHLPPSLHAGERSPERRDHSKL
                     SSVVEAQERALIIDALRETGGNQTKAAKILGTTKRVIQYKISKMGIDPGKFRKNTRDA
                     ALKP"
     gene            complement(2573..2914)
                     /locus_tag="PLR47_06375"
     CDS             complement(2573..2914)
                     /locus_tag="PLR47_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOG12607.1"
                     /translation="MKLIIAIVQSDKLKDVKQALRDVNVGKITVSTVVGCGAQVGKTD
                     TRDGVITNGDLLDRVRFEIAVNDDYVKPTLEAVIRGARTGSIGDGKIFIVPLEECVRI
                     RTGETGRLAIG"
     gene            complement(2943..3016)
                     /locus_tag="PLR47_06380"
     tRNA            complement(2943..3016)
                     /locus_tag="PLR47_06380"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2982..2984),aa:Gln,seq:ctg)
     gene            3243..3626
                     /locus_tag="PLR47_06385"
     CDS             3243..3626
                     /locus_tag="PLR47_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOG12608.1"
                     /translation="MNQLKKIEAIIREDKVNDVIDALKEIGIVGMNTFEVRGHGRQGG
                     VQLVGRAGTYQVNMLPKIQINIVLNTRNLDAALEAILCSAYTGETGDGIIFVTPVEDV
                     IRVRTRERGPGAMMYPGDIDERRSK"
     gene            complement(3627..4319)
                     /locus_tag="PLR47_06390"
     CDS             complement(3627..4319)
                     /locus_tag="PLR47_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOG12609.1"
                     /translation="MIARYFKHAPFEDIGSIRSWLTTAGYTITCTPFFQSANLPALEE
                     IDLLIVMGGPMSVNDEKRYPWLVPEKQFIRRMIISGKPVLGICLGAQLIAGAMGAKVY
                     PNGVKEIGWFPVHGVSSGGRSVFRFPQSVQAFHWHGETFDLPAGATRIAKSDGCENQA
                     FQLGQSVIGLQFHLETTPQSAQDLVAHCRDELIGAPYIQTETEILSAKPAQYQSINRL
                     MDKILNFLARTA"
     gene            complement(4429..5304)
                     /locus_tag="PLR47_06395"
     CDS             complement(4429..5304)
                     /locus_tag="PLR47_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12610.1"
                     /translation="MKKNIKRIMMIALLALMLTSMPMAAGAGEEPQSAGPKQQEDKLT
                     GEIAASVLSAYIWRGQEMTRDSVAIEPSFTANYKGFTAVLWGNLDTRPYAAGDGKHSS
                     TWSETDFILSYAHEFGIVKAGAGYIYYGLAGMTPLGVDLLDAQELFVSLGLNTILAPT
                     LTVYKEIDHYHQWYATLGVSHTFALHERVGLKLAAQASYLKSEDETTYPKFDSNSAAT
                     TDNFNNFHDGVATISLPVAVTGNLTITPTASCVFPLSEDAKYEMRARGLKGTANPSDR
                     DSYYVYGGVTLSLTF"
     gene            complement(5351..5692)
                     /locus_tag="PLR47_06400"
     CDS             complement(5351..5692)
                     /locus_tag="PLR47_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOG12611.1"
                     /translation="MKLVIAIIQPHKLEAVKRELEAVDVNLMTVTNVVGQGRQKGVTE
                     VYRGAKEAGGLLNKVRLDIAVNEAFVEPTIDAVTRGAHTGSVGDGKIFVVELAECVRI
                     SSGERGGVAIG"
     gene            complement(5705..>5992)
                     /locus_tag="PLR47_06405"
     CDS             complement(5705..>5992)
                     /locus_tag="PLR47_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HOG12612.1"
                     /translation="FGVHGVGGTVGTIATGLFAEKAVNAAGADGLLFGNLHQFGVQCL
                     MLVVTIAFAAIMTFIIFKIVDAAIGMRVEENNEIIGLDLTQQSEAAYTVIE"
BASE COUNT         1281 a         1663 c         1614 g         1434 t
ORIGIN      
        1 gccgttggtc atcgtcgcca tgccctggat gaaaaacggg tggcaggcgt aaatgttgat
       61 ggagtagttg gtgttctggc gcccctggca caggatcacc cgggcggtga gatccctgcg
      121 gtccagaccc agatactcgg cgacggccag cggatctccc acttccttga tcatgatcac
      181 gtccggccag aagctgaaga tcagcatgga ttgatctttt tcgccgatct gccgaagctg
      241 gagctgaacc atcatcaggc ggtattcgat ttcctcccgg cttaagtttt cccattcggc
      301 cgggtattcg taaacccgaa tgacgtagtt gtctctcttc ggcgttcccg aaggcgccgg
      361 tttggtcggc cgaagggaca gcttgtattt gaccatgaaa tcctgatcga tcatgaagtg
      421 gtccagggcc ttgatgcctt cattggaaaa gatgccggac aggatcggct cgtttttgaa
      481 tttttcgaac tcgccgccca gatcggtcag aaagagcccc acgccggagc cgtcatgccc
      541 ttctttcatg acattgagcg cgttgatggc catcaccggc gattgcggct gactggatgt
      601 tacggcaaat aaacggcaca tagtctctcc tttgaacctt tcaacccgag ggctcaactg
      661 ccagagtcgt gccagtgccg atcaaatgaa aaacaaacaa aaattataat ggattatata
      721 ggcttgccgc ggaagacgcc gcgggatgcg ggataaaaat caacaaattt gtgcttttgc
      781 gaaatggcgt gacattattg ttccatggat gaatttccgg cgcgcggaaa gccgcaaaag
      841 ccctatggct tcaaggcggc gtctcttgtg tttttgcgga attttccggg atcgatgccc
      901 attttcgata ttttatactg gatcacgcgc ttcgtggttc ccaggatttt ggccgccttg
      961 gtctggttgc cgcccgtttc cctgagcgcg tcgataatca gcgctctctc ctgcgcctcg
     1021 acgacggacg acagcttgct gtggtccctg cgctccggcg atctttcccc ggcatggagg
     1081 gaaggcggca gatgcaggca gtccacggtg tccgttttgg tcaggagcac cgcccgctcg
     1141 atgcagtttt ccagttcgcg gacattgccc ggccacttgt gggacagaaa cgcttcaatg
     1201 gcggccgtgg agatcttttt gacgttcttc cccatttcct tgctgtactt gagcagaaaa
     1261 tgatcggcca gcaggatgac gtcgctgccg cgttcccgaa gcggcggcag ataaacgggg
     1321 aaaacattga ggcggtaaaa caggtccgcc cggaaacggt cggcgagaat atcctgctcc
     1381 aggatgcggt tcgtggcggc gatgatcctc acgttgacgt tcaccaccct cgacgagccc
     1441 agcggctgaa atcgtttttc ctgcagaacg cgcagaagct ttacctgcgc ggcggcggac
     1501 agttcgccca cttcgtccag aaagatcgtg ccgccattgg cttcttcgaa gcgtccgcgg
     1561 cgcgtctgca gcgcgccggt gaacgcgcct ttctcatgtc cgaacagttc gctttcaatg
     1621 agcgtgtcgg gaatcgccgc gcagttgacc tgcaccagcg gcccgctttt gcgcggagag
     1681 ttgttgtgaa tcgcccgggc aatcaactct ttgcccgtgc ctgtttcgcc ggtgatcagc
     1741 actgtcgtgt tggaatcgga aacctgcgcc accaggccga agacttcctg gatggccttg
     1801 gaatggccga taatatccgt ggaaggcgcg tgctgctgtc cgacaatttt gcgcaagcgg
     1861 cggttttcct cttccagggc acggatgtga acggccttgg ccatcagttc ggccaccgag
     1921 cccatcatgg ccagttcctt ttccaggtcc tcgacctgct tggccacctt gtcggcggat
     1981 aaaaccccga ccacgcgctc gtcatagata atgggaacgc ataaaaagga aagctcggcg
     2041 cggttcaaat gacgtcttgc tcccgtgcgg tccagaaagt gggtttcctg ccccagattc
     2101 gccacggcca tcgggcggcc ggtctccgct acctggccgg tgattccttc tcccggtttg
     2161 tacgtcactt ccatgccgtc aatattgacg tcatgcgcga tgtcgagcca cgcttccttt
     2221 tcctcgtgat ccagaaggga gatcatgccg cgctgcattc cgaggcgcaa actcatttca
     2281 tggaggattt tttccagttg atctctcaaa tccatcggct gcgccaggat ttttgatatg
     2341 gcgtgcaggg ccgctaattc ttcataactg tggatgctca cgtcgtcttt ctcccccggg
     2401 cttttaccgg cgcttcacgg ccggattttc acggcccgta gaatgtaaca aaattgtacg
     2461 aatatcaacc ggaatttcat tcgtccgcgt acggtaatgc aaagagaaaa ccattatgtc
     2521 cgtttgatgc aattaaaaag aaaatgttat tttgcgggga tttcgtaaag atttatccga
     2581 tggccagccg gccggtttct cccgtgcgga ttcgaacaca ttcctccagg ggaacgatga
     2641 aaattttacc gtcgccgatg ctgccggtgc gcgctccccg aatgacggct tccagcgtgg
     2701 gcttgacgta gtcgtcgttc acggcaattt caaagcgcac ccggtcgagg aggtctccgt
     2761 tggtaatgac tccgtcgcgc gtgtcggttt tcccgacctg cgcgccgcag ccgactacgg
     2821 tggaaacggt aattttgccc acgtttacgt cgcgcaacgc ctgtttgacg tctttgagct
     2881 tgtcggattg cacgatggcg atgatcagtt tcatcagcat tggcctttca aaatggagtc
     2941 gatggctgag gggcaaggac tcgaacctca attttcaggg ccagaacctg gcgtcctacc
     3001 tttagacgac ccctcagtga aaaaacgaaa ccagcgtaag ctgataagcg cttcctttcc
     3061 cgacggataa caccaatgag ccgagagcaa tggccgtgcc agcagaagaa tttaaaattg
     3121 ttttatgcat tcaataggtt gagcaactct gttgattttc aaaagaatac acgtttgtcc
     3181 gttttgaaca cagacagaca taattgtatc cgctatgcgt tttcaggaaa gacgcagact
     3241 gaatgaatca gttgaaaaaa atcgaagcga tcattcgcga agataaagta aacgatgtta
     3301 tcgacgccct caaagaaatt ggaattgtgg gcatgaacac gtttgaagtg cggggacatg
     3361 gccggcaggg cggcgttcaa ctggtcggac gcgccggaac ctatcaggtc aatatgcttc
     3421 caaaaataca aatcaacatc gttttaaaca cgcgcaatct ggacgccgcg ctggaggcga
     3481 tcctctgctc ggcttacacg ggcgagacgg gcgacggcat tatttttgtg acacctgtcg
     3541 aagatgtcat tcgggttcgg acacgcgagc gtggtccggg cgccatgatg tatcccgggg
     3601 acattgatga acgcagaagt aaataatcaa gcggttcgcg ccaggaaatt caggatcttg
     3661 tccatcagac gattaatgga ttgatattgt gccggcttgg cggacaaaat ttccgtctcg
     3721 gtctgtatat agggcgcgcc gataagttca tcccggcaat gcgcaaccag gtcttgcgcg
     3781 gactgcggcg tggtttccag atgaaactgc aggccgataa ccgattggcc cagctgaaac
     3841 gcctgatttt cgcatccatc gcttttcgcg atgcgcgttg ctcccgccgg cagatcaaat
     3901 gtttctccgt gccaatggaa agcctgcacc gattgcggaa agcgaaagac ggatctgccg
     3961 ccggacgaaa cgccatggac gggaaaccag ccgatttctt ttacaccatt aggatacacc
     4021 tttgccccca tcgcgccggc gataagctgc gcgcccaggc agatgcccaa aaccggcttg
     4081 ccggaaataa tcattcggcg gataaactgt ttctccggaa caagccacgg atatctcttt
     4141 tcgtcattga cgctcatcgg cccgcccatc acaatcagca ggtcaatttc ctccaaagcg
     4201 ggcaggtttg ccgattgaaa aaacggcgtg caggtgatgg tgtagccggc cgtcgtaagc
     4261 cacgaacgga tgctgccgat atcttcaaag ggcgcgtgtt tgaaataacg ggcaatcatc
     4321 agatggtctc cttgatttta cctgcattga cgttgcgtaa ggcctgtatg acgtctttga
     4381 gcttgtcgga ctgtgctatg gcgatgacca gtttcatcat ggctctccct agaatgtcaa
     4441 acttaatgtt acgccgccat aaacgtagta actgtcccgg tcggatggat tggccgtgcc
     4501 cttcaggccc cgcgccctca tctcatattt cgcatcttcg gaaagcggaa acacacagga
     4561 ggcggtcggc gtaatggtca gattcccggt cacggcaacc ggcagggaaa tggttgcgac
     4621 tccgtcatgg aaattgttga aattgtccgt ggtggccgct gagttgctgt caaatttcgg
     4681 ataggttgtc tcatcttcac ttttcaggta gctggcctgc gccgccagtt tcaagccgac
     4741 cctttcatgc agggcaaagg tatgagaaac gcccagcgtc gcgtaccatt gatggtaatg
     4801 atcgatttcc ttgtagacgg tcagcgtagg cgccaaaatc gtgttgagac ccagcgaaac
     4861 aaaaagctcc tgggcatcca gcaggtccac tcccagcggc gtcattccgg ccagaccgta
     4921 ataaatgtat cccgcgcccg ccttcacgat gccgaattca tgcgcatagg aaaggatgaa
     4981 atccgtttca gaccaggtgg atgaatgttt gccgtccccg gccgcatagg ggcgggtatc
     5041 cagatttccc cagagaacgg cggtaaaacc tttgtagttt gctgtaaacg acggctcgat
     5101 tgccacgctg tcgcgcgtca tttcctggcc gcgccagatg taggcgctca aaacactggc
     5161 ggcaatctcg ccggtcaatt tatcttcctg ctgttttggt ccggcggatt ggggttcttc
     5221 ccccgcaccc gccgccatcg gcatggacgt cagcatcaac gccagcagag cgatcatcat
     5281 gattcttttg atgttttttt tcatgggtca ctgttctcct catgatttat attgtttcct
     5341 taaaatgccc tcacccgatg gcaacgccgc cccgctcgcc ggagctgatc cggacgcatt
     5401 cggccagctc cacaacgaaa atcttgccgt cgccgaccga gccggtgtgc gcgccgcggg
     5461 tgacggcgtc gatggtcggc tcgacaaacg cttcgttcac cgcaatatcg aggcggactt
     5521 tattgagcag gccgcccgct tccttcgcgc cgcgatagac ttccgtgacg cctttctgcc
     5581 ttccctggcc aacaacattc gtgaccgtca ttaaatttac gtccacggct tccagctctc
     5641 ttttgacagc ctccagtttg tgcggctgta tgatggcaat gactaatttc atggccagtt
     5701 cctcctattc aatcacggtg taagccgctt cgctttgctg ggtcagatcc aggccgatga
     5761 tttcgttgtt ctcttcgacc cgcatgccga ttgccgcgtc cacaattttg aagatgatga
     5821 aggtcatgat cgcggcaaag gcgatggtca ccacgagcat caggcattga accccgaact
     5881 ggtgcaggtt cccgaacagc aggccgtccg cgcccgccgc attgaccgct ttttccgcga
     5941 ataatcccgt ggcgatcgtt ccgaccgtcc cgcccacgcc gtgcacgccg aa
//
LOCUS       DAOSIM010000122         5932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_12103_length_5932_cov_7.644717, whole genome shotgun sequence.
ACCESSION   DAOSIM010000122 DAOSIM010000000
VERSION     DAOSIM010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12103_length_5932_cov_7.644717"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1782)
                     /locus_tag="PLR47_06410"
     CDS             complement(<1..1782)
                     /locus_tag="PLR47_06410"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG12613.1"
                     /translation="MIKLSLKRKIIGLAVMAAVLPVAVMMILMGSFKTSVSDLASKEL
                     SDVAMANMSQISRDVYGLCETSNDLIQRKINNGLNVARSILKQHGGIETAREKIAWDA
                     VNQLTRETQKIELPKLTAGGVWLGQNRSTSTPTPIVDDVKRLVGITCTIFQRMNENGD
                     MLRVATNVEDLNHRRAIGTYIPNTLPDGSANPVTAAVLKGQSYRGLAYVVNTWYLTAY
                     EPVTDRGGKIIGMLYVGEKLEAVASLRKAIMSMKVGKLGYVGVVGGKNEHRGRYIISK
                     DGKRDGENIWDQKDVHGNLTIQTLITKALAQPKGESFYHEYIWQNPGEPEPRKKISSV
                     IYFEPFDWVIVAGTYEDDFFRPIGHVHHMIKSIFLKMLLAGALIIFLSVLLAAVLARR
                     LTAPLELTTSLAQKIATGDIQQAREDLAGTASTRVIQAGDETGKLLQAFQAMTGNLDS
                     LIGQVHRSGIQVTTSATEIAASARELEATVAQQAASTTEVTATSREISATADELAQTM
                     DGVNESLNETIGMAQEGRSDLSRMETAMRELVKATSSISSKLTVINDRANRISHVVTT
                     INKISDQTNLLSLNAAIEAEKAGEFGRG"
     gene            2093..2848
                     /locus_tag="PLR47_06415"
     CDS             2093..2848
                     /locus_tag="PLR47_06415"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HOG12614.1"
                     /translation="MMDEVTIDEILGGRLKIRQSKRGYRFNLDSLMLAGFASIKPRSV
                     NLDLGCGNGILALVLAGRHPLTRWVGLEIQEGLANLARANVEQNGLAGRISIVAGDAR
                     NAGKIFSNHSFDQVVFNPPYRKISSGRINPLSEKAIARHELQGALADFLAASKILLKP
                     KGRVFTIYPAARLVELVSLFRARGIEPKRMKLVFSDASSDAVFVLAEGRSGSGEELKM
                     EPPLYIYEEGKTYTPEMKKLFSDLAAFPEACGG"
     gene            complement(2808..3302)
                     /locus_tag="PLR47_06420"
     CDS             complement(2808..3302)
                     /locus_tag="PLR47_06420"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOG12615.1"
                     /translation="MRRRFKGQLQPVGDILSSALKRRGLAGKLADNPVFKLWPQAVGA
                     KIARQTRPDGWRAGTLFVKTTSSVWVQQLHFMKEDIRKKINDLAGKTVVQEIHFGVGY
                     SPPRGDAGRLAVPPGTALKERDKRIIAECTETLKDPELAAVVKRVMQAEISRRRLREK
                     RQDR"
     gene            complement(3299..5287)
                     /locus_tag="PLR47_06425"
     CDS             complement(3299..5287)
                     /locus_tag="PLR47_06425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG12616.1"
                     /translation="MNAKPTYEELEQKVALLSRKVNEQQAVENQYRNLVDSTSDSLYL
                     VDADCRYLFINQNHARRLKMPQNDVAGLSYKDIHSPEQNAKFELKIKSVYQTGQSAFD
                     EHQSARDGRYFLRTFSPVRDSSSPEPIIAVAVVSKDITPRREAEEALRKSEEKYRQLI
                     EYSSEAIFIIANGKIGFANSRTEKMLGYPLADLQAMPFIDLVIAEDREMIAERQQAVL
                     QGTELSSNNHFRMVRKDESTIWVAANSVMMRWENASATLNFMRDITRQKKAEVRLLQA
                     QKMESIGTLAGGIAHDFNNLLMGIQGHTSLALLNLRPGDPNCEHIRTIEKLVISGAGL
                     TKQLLGFARGGKHEVKSVNLNDLIRQTSQTIGRTKKEIKIHHHLEDPLWPAEIDPEQI
                     EQVLVNLFINASQAMPAGGDLHLETKNQVVDENHFKDYSGKGGRFVRVSVTDNGVGMD
                     EKTRERIFEPFFTTKEMGRGTGLGLATVYGIIKNHNGFINVYSEKEHGSTFSIYLPAS
                     EKHAAKDRHSAGESAPEPSSGGETILVVDDEAMILDVAGEMLKMLNYRVITVPNGADA
                     IDLYRREQKRVDLVVLDMVMPDMNGGDVFDAMREINPDVKVLLSSGYSLNGQAGKIME
                     RGCSGFIQKPFTISDLAGKLRVVLEKPDPAAQCGSSKP"
     gene            complement(5305..5838)
                     /locus_tag="PLR47_06430"
     CDS             complement(5305..5838)
                     /locus_tag="PLR47_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOG12617.1"
                     /translation="MQITVNQTVIEIVCADITEEETDAIVNAANSTLSGGAGVDGAIH
                     RAGGPAIMAECRKIGGCPVGEAVITTGGNLKARYVIHTVGPVYRGGGRGEAALLASAY
                     RNSLKLAVKKGVGNISFPAISCGVYGYPVHEAASLALQTCLEFARSSAELKLIRHVLF
                     DERTYDIFAGKLKKLMT"
BASE COUNT         1080 a         1633 c         1790 g         1429 t
ORIGIN      
        1 gccccttccg aattcaccgg ctttttccgc ctcgatggcg gcgtttaacg acagcagatt
       61 ggtctggtcg gaaatcttgt tgatggtggt gacgacatgg gagatccggt tggcccggtc
      121 gttgatgacg gtcagctttg aagagatgga ggacgtggcc ttgaccagtt cccgcatggc
      181 cgtttccatc cgggaaaggt cgctgcggcc ctcctgggcc atgccgatgg tttcgttgag
      241 cgattcattg accccgtcca tggtctgggc cagttcgtcg gccgtggcgg agatttcccg
      301 gctggtggcc gttacttcgg tggtggaagc cgcctgctgc gccacggtcg cttcgagttc
      361 gcgcgccgaa gcggcgattt ccgtggcgga cgtggtgacc tggatgcccg agcggtgaac
      421 ctggccgatg agggagtcga gatttcccgt catggcctgg aaggcctgga gcagtttccc
      481 ggtttcgtct ccggcctgaa tgacccgcgt tgacgcggtt cccgccagat cttcgcgggc
      541 ctgctgaata tcgccggtag cgatcttctg ggcaagcgat gtcgtcagtt ccagaggggc
      601 cgtcagccga cgggccagga cagcggccag cagaaccgaa agaaagatga tcagcgcccc
      661 cgccaggagc attttcagaa agatggactt gatcatgtgg tgcacgtgtc cgatggggcg
      721 gaagaagtca tcttcatagg ttccggccac gatcacccag tcgaagggtt caaagtaaat
      781 gacggacgat atcttttttc tcggctcggg ctcgccggga ttctgccaga tgtattcatg
      841 gtagaaggat tcgcctttgg gctgggccag cgccttggtt atcagggtct gaatggtgag
      901 gttgccgtgg acatctttct gatcccagat gttttctccg tcccgcttgc cgtctttgga
      961 aatgatgtag cgcccccggt gctcgttctt gccgccgacg acgcccacat agcccagctt
     1021 tcccaccttc atgctcatga tcgcttttcg aagcgaagcg acggcttcca gcttttcccc
     1081 cacgtacagc atgccgatga ttttaccgcc gcggtccgtg acgggctcat aggcggtgag
     1141 gtaccacgtg ttgaccacat aggcgagacc ccggtaggac tgtcctttca ggaccgccgc
     1201 cgtcaccggg ttggcgctcc cgtccggcag cgtgtttgga atataggtgc cgatggcccg
     1261 gcgatggttc aggtcttcca cgttggtggc caccctcagc atgtcgccgt tttcgttcat
     1321 gcgctgaaaa atggtgcagg tgatgccgac cagacgcttc acgtcatcga cgatgggcgt
     1381 gggcgtagaa gtgctgcggt tctgtcccag ccagacgccg cccgccgtca gtttgggaag
     1441 ctcgattttc tgggtttccc gggtcaactg gtttacggca tcccatgcga tcttttccct
     1501 ggcggtttcg atgcccccgt gctgcttcag gatgcttctg gctacattca gcccgttgtt
     1561 gatttttcgc tggatcaggt cgttggaagt ttcgcacagt ccgtacacgt ccctggagat
     1621 ctgggacatg ttggccattg caacgtcgct aagctctttc gaggccaggt cggaaacgga
     1681 ggttttgaaa gatcccatga gaatcatcat gacggcgacc ggcagaacgg cggccatgac
     1741 ggcaaggccg atgatctttc gcttcagaga aagttttatc atggcgacgc atccttgatt
     1801 ggtatttttg cgcggcaaag tatagggaac gccgtggggt ctgtcaagaa aatcttggaa
     1861 aattcagggg gaagtccgcc gacctgttgc cggatggccg ggaaagacat tccccgcgca
     1921 tattatgtat tgacaggatg aagggattgc agttatcccc gtcatgtccg gaaaaacgaa
     1981 aaaaggaatt gcgttttcaa acgcggagcg tgaagggggc cggtttgttc acacggcgga
     2041 tttgccggat agggaagccg gcgggaaatt ccatgagcgg cggtgcggcg aaatgatgga
     2101 cgaagtgacg attgacgaaa tcctgggcgg acgcctcaaa atccgccaga gcaaaagggg
     2161 ctaccggttt aacctggatt ctctgatgct ggccggtttt gcctccatca aaccccggtc
     2221 cgtgaatctg gacctgggct gcggcaacgg aattctcgcg ctggttctgg ccggaaggca
     2281 tcccctgaca agatgggtcg gcctggagat tcaggagggc ctggcaaatc ttgcccgcgc
     2341 caatgtggaa caaaacggcc ttgcggggcg aatctcgatt gtcgcgggcg acgccaggaa
     2401 tgcggggaaa atcttttcaa accattcgtt tgaccaggtt gttttcaatc ctccctatcg
     2461 gaaaatttcc tccggccgaa tcaatccgct ctccgaaaaa gcgattgccc gccacgagct
     2521 tcagggggcg ctggcggatt ttctggcggc gtcaaagatt ctgctgaagc cgaaaggacg
     2581 ggtcttcacg atttatccgg cggcccggct ggtggagctg gtcagcctgt ttcgggccag
     2641 gggcatcgaa cccaagagaa tgaagctggt tttttccgac gcgtcatccg atgcggtctt
     2701 cgtgctggcg gaggggcgaa gcggttccgg tgaagagctg aagatggagc cgccgcttta
     2761 catatatgaa gaggggaaaa cctacacgcc ggagatgaaa aagttgttca gcgatcttgc
     2821 cgcttttccc gaagcctgcg gcggctgatt tccgcctgca tgaccctttt tacaacggcg
     2881 gccagttcgg gatctttgag cgtttcggtg cattcggcga tgattctttt gtctctctct
     2941 ttcagggcag tcccgggcgg gacggccagc cggcccgcgt ccccgcgggg agggctgtaa
     3001 cccacgccaa agtggatttc ctgcacgacc gtttttccgg ccagatcatt gatttttttc
     3061 cggatgtctt ctttcatgaa atgaagctgc tgcacccaga ccgaggaggt cgttttcacg
     3121 aagagggtgc ccgcgcgcca tccgtcggga cgggtttgcc gggcgatttt cgcgccgacc
     3181 gcctgcggcc agagcttgaa gacgggattg tccgccagtt tgccggccag gcctcttctc
     3241 ttcagggcgg aagaaagaat gtctcccacc ggctgcaatt gacctttaaa gcgtcttctc
     3301 atggctttga actcccgcat tgcgcggcgg gatccggttt ttccagaacc acccgcaatt
     3361 tcccggccaa atcgctgatc gtgaaaggct tctgaatgaa gccgctgcag ccgcgttcca
     3421 tgattttgcc ggcctggccg ttgaggctgt agccgctgga gagcagaact ttgacgtccg
     3481 gattgatctc ccgcatggcg tcgaaaacat cgccgccgtt catgtccggc atgaccatgt
     3541 ccagaacgac caggtcaact cgtttttgct cccgtcggta gaggtcgatc gcgtcggcgc
     3601 cgttggggac ggtgatcacg cggtagttca gcattttcag catttcgccg gccacatcca
     3661 ggatcatggc ttcatcgtcc acgaccagga ttgtttctcc gcccgaggat ggttcggggg
     3721 cggattcccc cgctgaatgc cggtcttttg cggcatgttt ctcggaggcc ggcaggtaga
     3781 tgctgaaggt tgagccgtgc tctttttcgg aatagacgtt gatgaagccg ttgtgatttt
     3841 tgatgatgcc gtaaacggtg gccaggccca gcccggtgcc ccggcccatt tctttggtgg
     3901 tgaaaaaagg ctcaaaaatc ctttcccggg ttttctcatc cattccgacg ccgttgtcgg
     3961 tgacggatac cctgacaaag cggccccctt tgccgctgta gtccttgaag tgattttcgt
     4021 ccacgacctg gttcttcgtt tcgagatgca gatccccgcc ggcgggcatg gcctgcgagg
     4081 cattgataaa cagattcacc agaacctgtt cgatctgctc cggatcgatt tccgcgggcc
     4141 acagaggatc ttcgagatga tgatgaatct tgatttcctt tttggttcgg ccgatggttt
     4201 gggacgtctg acggatcagg tcattgaggt tcacgctttt gacttcatgc ttgccgccgc
     4261 gggcaaagcc gagcagctgc ttggtgagcc ccgcgccgct gatgaccagc ttttcgatcg
     4321 tgcggatgtg ttcgcaattg ggatcgccgg ggcgaagatt caaaagcgca agcgaggtat
     4381 ggccctgaat gcccatgagc agattgttga aatcatgcgc gatgcctccc gccagcgtgc
     4441 cgatggattc cattttctgc gcctgcagaa gcctcacctc cgcctttttt tgccttgtaa
     4501 tgtcgcgcat gaagttcagc gtcgccgaag cgttttccca ccgcatcatg acgctgttgg
     4561 cggccaccca gatggtcgat tcgtccttgc gaaccatccg gaaatgattg tttgacgaca
     4621 gttccgttcc ctgaagaacg gcctgctgcc tctcggcgat catttcgcgg tcctcggcga
     4681 tcaccaggtc gatgaaaggc atcgcctgaa gatcggccag cggatacccc agcatctttt
     4741 ccgtgcggct gttggcaaag ccgattttcc cgttcgcgat gatgaaaatc gcctcgctgg
     4801 aatattcgat caactggcgg tatttttcct cggattttcg cagggcttct tccgcttccc
     4861 ttctcggggt gatgtctttg gaaaccaccg ccacggcgat gatgggttcg gggctggagg
     4921 aatcccgcac cgggctgaag gtgcgcagaa aataccggcc gtcccgcgcg ctctgatgct
     4981 cgtcgaaagc ggactggccc gtttggtaaa cgcttttgat tttaagctcg aatttggcgt
     5041 tctgctcggg cgagtgaatg tccttgtagg aaaggccggc gacatcgttt tgcggcattt
     5101 tcaggcgcct ggcatggttc tggttgataa acaggtagcg gcagtcggca tccaccaggt
     5161 acagggaatc gctggtggag tccaccaggt tgcggtattg attttcgaca gcctgctgtt
     5221 cgttcacttt ccgggacagc agggcgactt tctgctccag ttcctcgtag gtcggtttag
     5281 cgttcatgaa ggttcccgcg cctctcacgt cattaatttt ttcaactttc cggcgaaaat
     5341 gtcataggtc cgctcatcaa ataaaacatg gcgaatcagc ttcagctccg cgctgctgcg
     5401 cgcaaactcg aggcaggttt gaagcgccag ggaggcggct tcgtgcaccg ggtatccgta
     5461 aaccccgcag ctgatggccg gaaaggagat gttgccgacg ccttttttga cggccagctt
     5521 gaggctgttt cggtacgccg aagccagcag ggccgcctcg cctcgtcctc cgccgcggta
     5581 cacggggccg acggtatgga tgacatagcg ggctttgaga ttgccgccgg tcgtgatgac
     5641 ggcctcgccg acgggacagc cgccgatttt gcggcattcg gccatgatgg cgggtcctcc
     5701 ggcgcggtga atggccccgt caacgcccgc gccgcccgaa agtgtgctgt tggcggcgtt
     5761 gacgatggcg tcggtttctt cctcggtgat gtctgcgcaa acgatttcga tcacggtttg
     5821 gttcactgtg atttgcatgc tcattcctcc gcggggatgg gggttccctc tccgtaaacc
     5881 agcatccggt aggttttttc gatacggtcc cagttttctt cgcggtccat cg
//
LOCUS       DAOSIM010000123         5882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_12248_length_5882_cov_7.141411, whole genome shotgun sequence.
ACCESSION   DAOSIM010000123 DAOSIM010000000
VERSION     DAOSIM010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12248_length_5882_cov_7.141411"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PLR47_06435"
     CDS             <1..331
                     /locus_tag="PLR47_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOG12618.1"
                     /translation="AVGAGADVAIEAADITLVREDLNAVPEAIALSAATIRVIRQNLF
                     WAFIYNVIGIPIAAGILYPFFGILLSPAYAAAAMAFSSVSVVSNSLRLKRVWAKRSRL
                     RNEGDGI"
     gene            623..1174
                     /locus_tag="PLR47_06440"
     CDS             623..1174
                     /locus_tag="PLR47_06440"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOG12619.1"
                     /translation="MLLKGCLHTHTTCSDGKLSPQEVADAYESKGYDFIAFTDHDYLL
                     KPNYLETYGRVRTSMMVFYGIELSVFVNGNIHVAKIEGDREQLHVFNHISEYDFTPQQ
                     LIVRLRQLEKMYPLDAVEITKKGFRDSVFEALDLPYPKIASDDSHTLTGIGRAWVELD
                     AKREKDSIIHAIRHGEFWNCYLS"
     gene            1257..2402
                     /locus_tag="PLR47_06445"
     CDS             1257..2402
                     /locus_tag="PLR47_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOG12620.1"
                     /translation="MGKRLAVCAVAQIKNHPDYHDWRFQNMLLECFEPIVEETKVTFD
                     MDKGIRTIITCSDDMFDARTISDNGITDVVGAHFRGEEKMAQESINGLGYAMACILSG
                     HDDVILFMGHCKESQGESRRMISNLAYDPFYCRPLGMDYHNVDALQARAYMEKSKVTD
                     EHLAKVVVRSRANGKKNPYGRENKIVSEKDVLNSPMYSDPLREMHIYPVTDWAYGMLL
                     CSEKRAKEFTKKPVWITGYGSCMDRYFLGDRDIASNFSLKNAAKRAYAMAGIKDPRKD
                     VQVFELSDQAAYQLPMWAEGVGIAEEGRGGQWIDDGGMERFNVNLSGGQLNGNPLLLG
                     GAARALECYYQLSGQAGKRQVKGAKRAVAQAATGGAGQHQAVIVMEA"
     gene            2415..3614
                     /locus_tag="PLR47_06450"
     CDS             2415..3614
                     /locus_tag="PLR47_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12621.1"
                     /translation="MAHQKKNRNVGIIGIGQTKHSSHREDVNQPELIHEAVSLALKDA
                     NLTMKDIDCVVHGNMELFEMVHSPDLWHVLGTGAYGKDSIRITTGGTVGATLSCASDN
                     LVASGMYDIVMAIGFEKLQEGHTTGGITNMACPLWFRKIQAGALTGSSAYDVEREFGL
                     ERADRASLMYRIIMDKHASLNPNAHRAFGLDFSQIDDLIRTSPKLVGELKLIEMCSQS
                     DGACCVIYACEKKAKELSKIPVWIRDHITVHKEENFTIFGYDRKYPVLKTHKFAAEQL
                     FKRNGINDPLNYFDVFEMYDPATWWAIDWIRDFFNLQGDEHLRLIEEKEIMIGGKMPI
                     NPSGGVIGSNPIGATALVRVAEAALQVRGNAGPHQIPTPVNHALASGFGGTMWTVMTM
                     LEKELNW"
     gene            3625..4815
                     /locus_tag="PLR47_06455"
     CDS             3625..4815
                     /locus_tag="PLR47_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOG12622.1"
                     /translation="MAEYWGVKVEDIFNTMKDRFRPEGAKDVDAVFGYDIAGEGKWKL
                     TVKNNAMTLEKADQLSGCIATMSADGETFVGVNTGKVDATNAFMSGKFKVEGDMGAFG
                     KTGKLFRKFVLAKKEMTTKEYLADMFATIVPRFKAREAEGLEASFGFDLGGPDGGKWT
                     VFIKDKVCTVTETIEGKPTVTLEFNDARDYVDFILGKIDAQSILAAGKASAKGDINMM
                     ASKWPILFEKYKDPMAGAVQEQELLVLKKTISLNEKFSTGPIMGKFLKGLKEKKIFAN
                     RCPECGRLQLPPREVCAECRVRAGEFVEVGPKGEVRYMDVVYYAMPDPLTGVARETPY
                     GSIFIRLDGCKGNETLPHFIRKDQIEKIQRGWNEKKGTRVRPVWEEKRIGDIWDIKYF
                     EIDE"
     gene            4827..>5882
                     /locus_tag="PLR47_06460"
     CDS             4827..>5882
                     /locus_tag="PLR47_06460"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOG12623.1"
                     /translation="MTTKKTAGVEDSYQIEGKMALPYSYFAGRVGSKFITTLRDQKKI
                     MGVKCPACNTVYVPPRQVCDVDFTDIRDAWVELESTGTVKNFTVVRYDDKHLPRKAPF
                     VLALIQLEGAGTPFAHILEECKIEDVRIGMKVEAVFAKETTQTILDIDHFKPAAEKAS
                     IHEVNARRKQWVPTDEPAEASTRKGGKPDMSKPVIITAALTGAATMRNQNPSVPYKPE
                     EFAEEAYKCWKAGAAMVHVHAREDGGMATHDHARIKATYDAIKDKCPELIVCLSSAVG
                     MGKTAEQRISQIVYIKPDMASLNTNTMNFGIVDRKTGKIFIDYVFENTFGMLQDFGKV
                     MEENGVKPEIECYDMGGL"
BASE COUNT         1482 a         1496 c         1734 g         1170 t
ORIGIN      
        1 tgccgttgga gcgggtgcgg atgtggccat tgaggcggcg gacatcacgc tggtgcggga
       61 ggatttgaat gccgtgccgg aagccatcgc cctttcggcg gcgacgatac gggtgattcg
      121 ccaaaatctg ttttgggcgt ttatttacaa tgtcatcggc attccgattg cggcggggat
      181 tctttacccc ttcttcggga tcctgttaag tcctgcatat gcggcagccg ccatggcttt
      241 cagctcggtt tccgtcgtga gcaattccct gcggctgaaa agggtttggg caaagcgaag
      301 ccgcttgagg aatgaagggg atgggatata ggtcataggc catgggttat gggctatagg
      361 gcatagggcg ttgggacggg tttttcgcca atgggtgatt gacggcgcca ggatgaatag
      421 aaacccctaa caaattcaat catctccaat tatttaccaa aaatgaattt tttttcttga
      481 caatcaggaa cgggaagagt aatcgaatag ccattggttg aacgtccaat caagaattgg
      541 gttgatcgtc caataaggat cagaaacatc atggacgcag gttttcggct tattggttac
      601 gggaatcatt ttgaaggttt ctatgctgtt aaaaggctgc ttacacaccc acacaacctg
      661 ttcggacgga aaattatccc cgcaggaagt tgcggatgct tatgagtcaa aaggctatga
      721 tttcattgcc tttacggatc atgattacct tttgaagcca aactatctgg agacttacgg
      781 ccgggtcagg acaagcatga tggttttcta cggcatagag ctttcggttt ttgtcaacgg
      841 aaacattcat gtggcaaaaa ttgaaggcga ccgggagcag ctccatgtct tcaatcacat
      901 cagcgagtat gatttcacac cccagcagct gatcgtgcgt ctgcggcagc tcgaaaaaat
      961 gtatcccctg gatgcggtgg aaatcacgaa gaaaggattc cgcgattcgg tttttgaagc
     1021 gctcgatctt ccctatccca aaatcgcctc cgatgactcg cacacactga cggggattgg
     1081 ccgggcgtgg gtagagctgg atgcgaagag ggaaaaggat tccattattc acgcgatccg
     1141 gcacggtgaa ttctggaact gttacctgag ttgagtacgg cggccgcttt cgggcggacg
     1201 ccgcaaatac ggatcagtca ttattcggtc atatgaaaca ggaaggagga cggggaatgg
     1261 gaaaaagatt ggcggtctgc gcagtggcgc agattaaaaa ccatccggat tatcatgatt
     1321 ggcgttttca aaacatgctg ctcgaatgtt tcgaaccgat cgtggaggag acaaaggtga
     1381 ccttcgacat ggacaagggc atccggacga tcatcacctg ctcggatgac atgttcgatg
     1441 cccggaccat atccgacaac gggattacgg atgtcgtcgg ggcgcatttt cgcggcgagg
     1501 aaaagatggc acaggaaagc atcaacggcc tcggatacgc catggcctgc attctctccg
     1561 gccatgacga cgtgatcctt tttatgggcc actgcaagga gtcgcagggg gaaagccgca
     1621 ggatgatttc caatcttgcc tatgatccct tctactgcag accgctgggc atggactacc
     1681 acaacgtgga cgcgctgcag gcccgcgctt acatggaaaa atcaaaggtg acggacgagc
     1741 atctggcaaa agtggtggtc cgctccagag cgaacggcaa aaaaaatccc tatggccgcg
     1801 aaaacaaaat cgtcagtgaa aaagacgtgc tgaattcacc gatgtactcg gatcccctcc
     1861 gggaaatgca catctatccg gtcaccgact gggcctacgg aatgcttttg tgcagtgaaa
     1921 agcgggccaa ggagttcacc aagaaacccg tctggatcac aggatacggc agctgcatgg
     1981 accggtattt cctcggcgac cgcgacatcg cttcgaattt ttcgctcaag aacgccgcca
     2041 agcgggcgta cgccatggcc ggcattaaag atccccgaaa agacgtccag gtttttgaac
     2101 tgagcgatca agctgcatat cagctcccga tgtgggccga aggcgtcggc atcgcggaag
     2161 aaggacgggg cggccagtgg atcgacgatg gcggcatgga acggttcaat gtcaacctgt
     2221 ccggcggcca gctcaacggc aatccgcttc ttctgggcgg cgcggccagg gctctggaat
     2281 gctactacca gttgtcgggg caggcaggca agcggcaggt caagggtgca aaacgggcag
     2341 ttgcccaggc ggcgaccggc ggggccgggc agcatcaggc cgtcatcgtc atggaagcgt
     2401 agggaggttg aatcatggca catcaaaaga aaaacagaaa tgttggaatt atcggcatcg
     2461 gtcagaccaa acattccagt caccgcgagg atgtcaacca gcctgaattg atccatgaag
     2521 cggtcagcct ggccctgaaa gacgcgaacc tcaccatgaa agacattgac tgcgtggttc
     2581 acggcaatat ggagcttttt gaaatggtcc actcgccgga tctctggcac gtgctgggaa
     2641 ccggcgccta tggcaaggac tcgatccgga tcactaccgg cgggacggtc ggggccactc
     2701 tgtcgtgcgc ctcggacaat ctggtggcgt cgggaatgta cgacatcgtc atggccatcg
     2761 gatttgaaaa gcttcaggag gggcacacca cgggcggcat caccaacatg gcctgcccgc
     2821 tgtggttcag gaaaattcag gcgggagcgc tcaccggctc ctcggcctat gacgtggagc
     2881 gggaattcgg cctggagcgg gcggatcgcg catcgctcat gtaccggatc atcatggaca
     2941 agcacgcaag cctcaatccc aacgcccacc gggcattcgg cctggacttc agccagatcg
     3001 acgatctgat ccggacttcg ccgaagcttg tcggcgaact gaagctcatc gaaatgtgct
     3061 cgcagtcgga cggcgcctgc tgcgtgatct acgcgtgcga gaaaaaggcg aaagagctgt
     3121 ccaaaattcc ggtctggatc cgcgaccaca tcaccgtcca caaggaagag aacttcacga
     3181 tcttcggcta cgaccggaaa tatccggtgc tgaaaacgca taaattcgcg gccgagcagc
     3241 ttttcaaacg caacggcatc aatgaccctt tgaattattt tgacgtgttc gaaatgtatg
     3301 atcccgcgac ctggtgggcc atcgactgga ttcgggactt cttcaatctg cagggggacg
     3361 agcacctcag actcatcgag gaaaaggaga tcatgatcgg cggcaagatg cccatcaatc
     3421 cgtccggcgg cgtgatcgga tccaatccga tcggcgcgac ggcgctggtc cgggtggccg
     3481 aggcggccct gcaggtgcgc ggcaatgccg gcccgcacca gattcccacc ccggtcaatc
     3541 atgcactcgc gtcagggttt ggcggcacca tgtggaccgt gatgacaatg ctcgaaaagg
     3601 aacttaactg gtaggaggac gatcatggca gaatactggg gagtaaaggt agaagatata
     3661 ttcaatacga tgaaagaccg gttccgcccc gaaggcgcca aggatgtcga tgcggttttc
     3721 ggctatgaca tcgccgggga aggcaaatgg aagctgacgg taaagaacaa tgcgatgacg
     3781 ctcgaaaagg cggatcaatt atccggctgc atcgcaacca tgtcggccga cggcgagacc
     3841 tttgtcggtg tcaacacggg caaagtcgat gcgaccaacg cctttatgag cggaaaattc
     3901 aaggtggaag gcgacatggg cgccttcgga aagaccggga aacttttccg caaattcgtc
     3961 ctcgccaaaa aggagatgac gaccaaagag tatcttgcgg acatgttcgc caccatcgtg
     4021 ccccgcttta aggccaggga ggccgaaggg ctcgaagctt ccttcggttt cgatctcggc
     4081 ggaccggacg gcggcaagtg gaccgtcttt attaaggata aggtctgcac ggttacggaa
     4141 acaattgaag gcaagccgac ggttacgctg gaattcaacg acgccaggga ttacgttgat
     4201 ttcatccttg gaaagatcga cgcgcagagc atcctggccg cgggaaaagc gtccgcgaag
     4261 ggcgatatca acatgatggc gtcgaaatgg ccgatcctgt ttgagaaata caaagatccc
     4321 atggccggcg ctgttcagga acaggaactg ctggtgctca aaaagaccat atcgttgaac
     4381 gagaagtttt caaccggccc catcatgggc aagttcctca agggcctgaa agagaaaaaa
     4441 atattcgcga atcgatgccc cgagtgcggc cggctgcagt tgccgccgcg ggaagtgtgc
     4501 gcggaatgcc gcgtgcgcgc cggtgagttc gtggaggtcg ggcccaaggg ggaagtccga
     4561 tatatggacg tggtctatta cgccatgcct gatcccctga ccggcgtggc ccgggaaacc
     4621 ccgtatggct ccatctttat ccgactggat ggctgcaagg gcaacgagac cctgccgcac
     4681 tttatccgga aggatcagat cgaaaagatt cagaggggct ggaatgaaaa gaaagggacc
     4741 cgcgtccgcc ccgtctggga ggaaaaacgg atcggagaca tctgggacat taaatacttt
     4801 gagattgatg agtaaggaga agcgatatga caaccaaaaa gaccgcagga gtcgaagaca
     4861 gctatcaaat cgaaggcaaa atggcgctcc cttattctta ctttgccggc cgggtgggca
     4921 gcaaattcat tacgaccctc cgggatcaga agaaaatcat gggagtgaaa tgccccgcct
     4981 gcaatacggt ctatgtgccg ccgcggcagg tctgcgacgt ggactttacc gatattcgcg
     5041 acgcgtgggt tgaattggaa agcacgggca cggtgaagaa tttcacggtc gtgcgttatg
     5101 acgacaagca tctgccgaga aaggcgccgt ttgtcctggc cctcattcaa ctggaggggg
     5161 ccggcacgcc gtttgcgcac attctggagg agtgcaaaat cgaagacgtc aggatcggaa
     5221 tgaaggtcga ggcggtattc gcgaaggaaa cgacccaaac cattcttgat atcgatcact
     5281 tcaaacccgc ggcggaaaag gcgtcgattc atgaggtgaa cgcgcgcagg aaacaatggg
     5341 ttccgaccga tgaacccgcc gaagcatcaa ccagaaaagg agggaagccc gatatgtcca
     5401 agcccgttat tattaccgcg gcgctcaccg gcgccgctac gatgagaaat cagaacccgt
     5461 ccgtacccta caaaccggag gaattcgctg aagaagccta caagtgctgg aaagccggcg
     5521 ccgcgatggt gcatgtgcat gcccgggaag acggaggaat ggccacccat gaccacgcga
     5581 ggatcaaggc gacctatgat gccatcaagg ataagtgtcc ggaactcatc gtctgtctga
     5641 gctccgccgt cggcatgggc aagaccgccg agcagcggat ctcccagatt gtctatatca
     5701 aacccgacat ggcctcgctc aacaccaaca ccatgaactt cggcatcgtg gaccgcaaaa
     5761 ccggcaagat cttcatcgac tatgtgtttg aaaacacttt cggcatgctg caggacttcg
     5821 gcaaagttat ggaagaaaac ggcgtgaagc cggaaatcga gtgctacgac atgggcggtc
     5881 tg
//
LOCUS       DAOSIM010000124         5881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_12250_length_5881_cov_8.889633, whole genome shotgun sequence.
ACCESSION   DAOSIM010000124 DAOSIM010000000
VERSION     DAOSIM010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5881
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12250_length_5881_cov_8.889633"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="PLR47_06465"
     CDS             <1..1102
                     /locus_tag="PLR47_06465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG12624.1"
                     /translation="NAKKSLDRKKTVASFCMGSRDTYRFLNDNPAFEFRPIDYTNSLF
                     VIGRQANMTAINSALEIDLTGQATAESIGGVFYSGVGGQADFMRGSALAPGGKSILAM
                     RSTSIDMAHSRIVPCLSAGASATLIRGDVHYVVTEYGTAYLHGKNIRERAMALISIAH
                     PRFRPWLVEQARERGLIYRDQAFIPGTTGVYPEHLETYRSTRTGLPLFLRPVKISDEG
                     IIKAFLYSLSDKSIYTRFFTNMVHMPHNILQKYVIIDYSKEMLMLAIQERQGMEEIVG
                     MGQYIIDTDTHTAAASFLVGDVWQNRGIGIVLLEYLTEIAKRDGLLGFSATVLAENKA
                     MLRLFERMNFQMEKQFSSGVYELTMGFYPREP"
     gene            1219..2316
                     /locus_tag="PLR47_06470"
     CDS             1219..2316
                     /locus_tag="PLR47_06470"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOG12625.1"
                     /translation="MKKVLFMMVLLLVLFAGYFLFWPVPIEPVAWNAPAAAGYTGPHA
                     FNTGLSRLNLIDLGAEEGPEHIVLGKDGKLYVSVGSGNVLRMNPDGSGQEVFIRTGGR
                     VLGFDFDKAGNLIAADAIRGLLSVSPQGKITLLTRSVNGDPIRYADAVVVARSGMIYL
                     SDASRRFSPAEWGGPFEASILDIMEMSSTGRVLAYDPAQKSTRVVASGFCFANGLALS
                     RDEQNLFVNETGKYRLWKVSVHALELDIARPGDQAKIVFDNLPGFPDNLMRGQDGKIW
                     MGFAKPRNPDLDQMGSKPFLRKITLRLPRSLWPVPKAYGHVFAFTEDGRVVADLQDPG
                     GAYPETTGVTETADRLYIQSLHAKGLGWLPK"
     gene            complement(2355..3047)
                     /locus_tag="PLR47_06475"
     CDS             complement(2355..3047)
                     /locus_tag="PLR47_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOG12626.1"
                     /translation="MMNKVRNVLTQSQLFGGLPDEHLAEIENIAVPRRCGKGEMVFHD
                     GDEGIGFYLVVAGIVNVYKLSSEGKEQILHIVGEGDTIGAVPVFSGESFPANARAVTP
                     CHLLFFNREKFIRLITRQPSLTMNILALLSARLREFTVQVENLSLKEIPGRLASYLLD
                     LAREQGNRDRIALNISKLQLAGLLGTGPESLSRALGSLKSKKLIEEKGSMIRLVNRAL
                     LEELAEKGKNTP"
     gene            3161..4795
                     /gene="hcp"
                     /locus_tag="PLR47_06480"
                     /gene_synonym="priS"
     CDS             3161..4795
                     /gene="hcp"
                     /locus_tag="PLR47_06480"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF003658.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOG12627.1"
                     /translation="MFCYQCQETVKNEGCTVKGVCGKTEETSNLQDLLIYLLNGMAVC
                     ADKAKEKGPLDPKYGRFMMEALFATITNANFDSDRIIGMIREALRLRSELRQACGAAF
                     PAGLHDCAVWDSDNMEEFKKKAAAVGVLSTENEDVRSLRMLLLFGIKGIAAYAEHAAV
                     LGFEDGKIHDFVREALISITEDLSVDEMVAMVLRAGECAVSAMALLDKANTSTYGHPE
                     ISKVNIGVRGNPGILISGHDLKDLDELLKQTEGTGVDVYTHGEMLPANSYPAFKKYAH
                     FVGNYGNAWWKQNEEFESFNGPILMTTNCIIPVRDSYKDRIFTTGVVGYPGLKHIPDR
                     SKGGTKDFSEIIELAKKCAPPRELEKGELVAGFAHHQVLALADQVVAAVKSGAVKRFI
                     VMAGCDGRHKSRGYFTDVARELPEDTIILTAGCAKYRYNKLALGDIGGIPRVLDAGQC
                     NDCYSLALIALKLKEVFGLKDINELPISFDIAWYEQKAVAVLLALLYLGVKGIRLGPT
                     LPAFASPNVLKVLVEKFNIKPITDSKTDVEAMVEGR"
     gene            4905..5639
                     /locus_tag="PLR47_06485"
     CDS             4905..5639
                     /locus_tag="PLR47_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG12628.1"
                     /translation="MKKILRKIIEIDEALCDGCGQCVTGCDEGALQIIDGKAKVVSET
                     FCDGLGACIGQCPTGALRIVEREASPFDEEAVQKHLAGQSQPPLPCGCPSAQIQILGK
                     PSACEAANQPKHLEEGEEESALTHWPIQIKLVPASAPFLKGADLLVLADCTAVAFPLL
                     HRNLLKGRVVMMGCPKFDDVQEYVQKFADIFQTANIKSVTVAVMEVPCCSGMPVIVRK
                     GMEAAKRIIPMTEIVLSLRGKILKQS"
BASE COUNT         1333 a         1688 c         1650 g         1210 t
ORIGIN      
        1 caacgcgaag aaatccctgg accgcaaaaa aaccgtcgcg tcgttctgca tggggagccg
       61 cgacacctac agattcctca acgacaaccc cgcgtttgag ttcaggccga tcgactatac
      121 caacagcctc tttgtcatcg ggcggcaggc caacatgacc gccatcaact ccgcgctgga
      181 aattgatctc acggggcagg ccaccgcgga atcgatcggc ggagtctttt acagcggcgt
      241 cggcggacag gcggatttca tgaggggctc ggcgcttgcg ccgggcggca agtccattct
      301 ggccatgcgt tccacctcga tagacatggc tcattcccgc atcgtcccct gcctttccgc
      361 cggcgcgtcg gccacgctca tccgcgggga cgtgcactat gtggtcaccg aatacggaac
      421 ggcctacctg catggaaaaa atatccggga acgcgccatg gctctcattt ccatcgcgca
      481 cccgaggttc cgcccctggc ttgtggagca ggcgcgcgaa cggggcctca tctaccgcga
      541 ccaggcgttt atccccggca cgacgggcgt ttatcccgag catctggaaa cctaccgctc
      601 cacgcggacc ggcctgccgc ttttcctccg ccccgtcaaa atcagcgacg agggaatcat
      661 caaggccttt ctctactcgc tttcggacaa gagcatctac acacgttttt tcaccaacat
      721 ggtccacatg ccgcacaaca tccttcaaaa atacgtgatc atcgattact cgaaagaaat
      781 gctgatgctc gccatccagg agcggcaggg catggaggag atcgtgggta tgggccagta
      841 catcatcgac acggacacgc acacggccgc cgcctccttt ctggtgggcg acgtctggca
      901 gaaccgcggc atcggaatcg tgctcctcga atatctcacc gaaatcgcga agcgggacgg
      961 gcttctgggc ttttccgcca cagtgctggc ggaaaacaaa gccatgctcc gtcttttcga
     1021 gcgaatgaat tttcagatgg aaaagcagtt ttcgtcgggc gtatatgagt tgaccatggg
     1081 cttttacccg agagaaccct gaacgtttcg tttcccgttt tttcacaaag acacaagacc
     1141 ctgagcggca agacagtgcg gtccaaaact ttttgatccg tcaaccggcc caaccagcat
     1201 tatcggaagg aggtcctgat gaaaaaagta ctgttcatga tggttttgct tctggtcctt
     1261 ttcgcggggt atttcctttt ctggccggtg ccgatcgagc cggtcgcgtg gaatgcgccg
     1321 gctgcggccg gatataccgg accgcatgcg ttcaacaccg gattgtcccg gctgaacctc
     1381 atcgacctgg gcgccgaaga ggggcccgaa cacatcgtcc tgggaaaaga cggaaagctg
     1441 tatgtctccg tgggcagcgg aaacgttctg cgcatgaatc ctgacggaag cggacaggag
     1501 gttttcatcc gcaccggcgg gcgcgtactg ggatttgatt tcgacaaagc ggggaacctg
     1561 attgccgcgg atgcaatccg gggactgctg tccgtcagcc ctcaagggaa gatcaccctg
     1621 ctgacccggt cggtcaacgg cgacccgatc cgctatgccg acgcggtggt ggttgcccgg
     1681 tccggcatga tttacttaag cgatgcttcg aggcgctttt cacccgccga atggggcgga
     1741 ccgtttgaag ccagtattct ggacatcatg gaaatgtcct ccaccggacg cgtcctcgcc
     1801 tacgatccgg cgcaaaaatc cacccgcgtc gtcgccagtg gattctgttt cgccaatggt
     1861 ctggccctca gccgggacga gcaaaatctt tttgtgaacg aaacgggaaa ataccggctt
     1921 tggaaagtgt ccgtacacgc gctggaactg gacattgccc ggccgggcga tcaggctaaa
     1981 attgttttcg acaatcttcc cggctttccc gacaacctga tgcgcggaca ggatggaaaa
     2041 atctggatgg gctttgccaa accgcgcaat cccgatctgg accaaatggg aagcaagccc
     2101 tttctccgaa agattaccct gcgtctgccg cgttccctgt ggccggttcc caaagcttac
     2161 gggcatgtgt tcgccttcac ggaagacggc cgggtcgtgg ccgacctgca ggatcccggc
     2221 ggcgcgtatc cggaaaccac cggcgtgacg gaaaccgccg accgccttta tattcagagc
     2281 ctgcatgcca aagggctcgg ctggctgccc aaataaagga gtttccgaaa gcaaacccgc
     2341 cgaagccggg cgtgctatgg cgtgtttttt cctttttcgg ccagctcttc caagagcgcc
     2401 cggttcacga gccggatcat ggatcccttt tcctcgatca gctttttgct tttcaggctg
     2461 cccagagcgc gggaaagaga ttccggtccg gtgccgagca ggccggccaa ttgcagtttg
     2521 gaaatattga gcgcgatccg atcccggttt ccctgctccc gcgcgagatc cagaaggtag
     2581 gaagccagac ggccgggaat ttccttgagc gacagatttt ccacctggac ggtaaactcc
     2641 cgcaggcggg cggacaggag ggccaggata ttcatcgtaa gagacggctg acgggtaatc
     2701 aggcggatga atttctcccg gttgaaaaac agaagatggc agggggtcac cgcgcgggcg
     2761 ttggcgggaa aggattcccc ggaaaaaacg ggaaccgcgc cgatggtgtc tccctccccg
     2821 acgatatgaa ggatctgctc cttcccttcg gaagaaagtt tataaacatt gacgatcccc
     2881 gcgaccacca gataaaagcc gattccttca tctccgtcat gaaagaccat ctcccccttg
     2941 ccgcagcgcc tgggaaccgc tatgttttca atctccgcaa gatgctcatc cggcagtccc
     3001 ccgaaaagct ggctctgggt caaaacgttc cgaactttgt tcatcattgg cgtcctctcg
     3061 gcaaaaaatg tttttttact taacataaat caaggatgtc cggaatgatt ttcagtattc
     3121 tttccggcaa atcgcaacga atcgtaacag gaggtgtcac atgttttgtt atcagtgcca
     3181 ggaaaccgtc aaaaatgaag gatgtacggt caaaggggtc tgcggtaaaa ccgaggaaac
     3241 atccaatctg caggaccttc tgatttatct tctgaacggc atggccgtct gtgccgacaa
     3301 ggctaaagaa aaaggtccgc tggatcccaa atacgggcgc tttatgatgg aggcgctttt
     3361 tgccacgatc accaatgcca acttcgacag cgaccggatc atcggaatga ttcgggaggc
     3421 gcttcggctt cgcagcgaac tcagacaagc ctgcggcgcg gcgtttcccg cgggcctgca
     3481 tgactgtgcc gtgtgggatt ccgacaacat ggaggaattt aaaaagaaag ccgccgccgt
     3541 cggtgttctt tccacggaaa acgaggatgt gcgctcgctc cgaatgctgc tcctcttcgg
     3601 aatcaaggga atcgcggctt atgcggaaca tgccgccgtt ctgggttttg aagacgggaa
     3661 aattcatgat ttcgtcaggg aagcgctgat ctccatcacc gaagatttga gcgtggatga
     3721 aatggtggcc atggtcctgc gcgcgggcga atgcgccgta agcgcgatgg cactcttgga
     3781 caaggccaac acctcgacat acggccatcc cgaaatttcc aaagtcaaca tcggcgtgcg
     3841 gggcaacccg ggcattctga tttcgggcca tgacctcaag gacctcgacg agctgctgaa
     3901 acagacggag ggaaccggcg tggatgttta tacacacggc gagatgctgc cggccaacag
     3961 ctacccagcg ttcaagaaat acgcccactt cgttggaaat tacggaaacg cctggtggaa
     4021 gcagaatgag gagttcgaat ccttcaacgg ccccatcctg atgacgacca actgcatcat
     4081 tccggtcagg gattcctata aagaccgaat cttcacgacg ggcgtcgtgg gctaccccgg
     4141 attgaaacac atccccgaca gatccaaagg cggaacaaaa gatttttctg aaatcatcga
     4201 gcttgcgaaa aaatgcgcgc cgcccaggga gttggaaaaa ggcgaactgg tggcgggatt
     4261 tgcccatcat caggtgctgg ccctggcgga tcaggtggtc gcggcggtga aatcgggcgc
     4321 cgtcaagcgc ttcatcgtca tggccggctg cgacgggcgg cacaagtccc gcggctactt
     4381 caccgatgtc gcccgggaac tccccgaaga cacgattatc ctgaccgccg gctgcgccaa
     4441 gtaccgctac aacaagcttg cgctgggcga tatcggcggc attccccgag tgctggatgc
     4501 cggacagtgc aatgactgct actcgctggc gttaatcgcg ttgaagctca aagaagtttt
     4561 cggactgaaa gacatcaacg aactgccgat ttcctttgac attgcctggt acgagcagaa
     4621 agcggtggcg gtgcttctgg ctcttctgta cctgggtgtc aagggaatcc gcctcggacc
     4681 gaccctgccg gccttcgcgt cgccgaacgt cctcaaggtc ctggtggaga agttcaacat
     4741 caagccgata acggattcaa agacggatgt cgaagccatg gttgagggcc gttagttcat
     4801 tttcccgctt tcaggggaaa ggcgcattca agcgcccgct cctcctttgc acacccctgg
     4861 cagcggggat gttttctgaa aatcaagagc caaaagggaa aaacatgaaa aaaatattgc
     4921 gtaaaatcat tgaaatcgac gaggcgctgt gcgacgggtg cggccagtgc gtgacgggct
     4981 gcgacgaggg cgccctgcaa atcatcgacg gcaaggccaa agtcgtttcg gaaacattct
     5041 gcgacggtct gggcgcctgc atcggccagt gccccaccgg ggcgctccgg atcgtcgagc
     5101 gcgaggcgtc gccctttgac gaagaggccg tccaaaagca cctggccgga caaagccaac
     5161 cgccgcttcc ctgcggctgc ccttccgctc agattcaaat tctcggcaaa ccgtccgcct
     5221 gtgaagcggc caatcagccg aagcatctgg aggagggcga agaagaatca gccctcaccc
     5281 actggcccat acagatcaag ctggtgccgg cttccgctcc tttcctgaag ggagccgacc
     5341 tgctggtcct tgcggactgc acggcggtcg cttttcccct tctgcaccgg aatctgctga
     5401 aaggaagagt ggtgatgatg ggctgcccga aattcgacga cgtgcaggaa tacgttcaaa
     5461 agtttgccga tatttttcaa acggcaaaca tcaaaagcgt cacggtggcc gtcatggaag
     5521 tcccctgctg ttcgggcatg cccgtcatcg tccgaaaagg catggaagcg gcaaagcgaa
     5581 tcatccccat gacggaaatc gtgctgagtt taagaggaaa aatcctgaaa caaagctgac
     5641 ggcttatgat cggccgcgtc ggatgagccg tccccggaaa accggggacg gccgcccggc
     5701 cttaggccag tcccttctta ttgatgccgt ccgcgacctc gtccaaaaat tgttttcatt
     5761 ttaaaaagca ttccattttc ctcctttgta aattcagcaa gtgagtctca cggccgttta
     5821 aaaccgctgg cccacattga gataaatgga cgaatggcct cgctgggcct gaccataggc
     5881 c
//
LOCUS       DAOSIM010000125         5846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_12354_length_5846_cov_8.408565, whole genome shotgun sequence.
ACCESSION   DAOSIM010000125 DAOSIM010000000
VERSION     DAOSIM010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12354_length_5846_cov_8.408565"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PLR47_06490"
     CDS             <1..764
                     /locus_tag="PLR47_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12629.1"
                     /translation="IIFQCVQFYRNFSKMFVHEKITEEVQKYFRIYATECYALGIPMA
                     EMVYALILMRRHIWLYAEFQMIFSSLINQQQALDTLNRTILLFDYASYDVTREYQELA
                     KRDKLESIRLLDILESNVVEIASNWAATVRKDKQTVFYHGIPKDKLIPQAIKFYSRLR
                     TLLFDPERFEKGREFFRQYAETCRQQGIPLHEAIYALNVMRRQMWLHGEFQRTFVNAL
                     AHQQAVDSLMRIILLMDYAAYDITHYYQERMLLED"
     gene            1286..2272
                     /locus_tag="PLR47_06495"
     CDS             1286..2272
                     /locus_tag="PLR47_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOG12630.1"
                     /translation="MNALVTGATGFIGSVLVRDLLARGSSVRALVLPGENADVLKKQG
                     VRVIRGDLTDPESLRGVCEGIETVFHLAGRVTDWGTKRQFYTAICDATGNLLQEAAGR
                     ASRFVYVSSIAAMGMGRHLKGVKESDPPRKSGVSYNDAKVDAEALVRAFHREDKMKCT
                     IVRPANVVGPGSVWVRDIADRMMSATGVPLIDGGRYSASFVYVDHLVDGMILAAAGDI
                     ACGKAYHFRDDWRVNWKEYITDLGSWIGKRPRGSIPFRLAWMTGFVLEAVLNPLHIRA
                     PLTRLSAGVLGRDNDVDTMLARTELGWRTRLSYPEAMEKIGAWVKDHYQIGR"
     gene            2275..2634
                     /locus_tag="PLR47_06500"
     CDS             2275..2634
                     /locus_tag="PLR47_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12631.1"
                     /translation="MKLRLLLFILYQKLKKAAAKNAAYRSFLGGMQVKIMIQTADGKH
                     GRLFVFDRGKLSTLSGCGHSYDAALVWSDSGTAFQVMSSGSDEQTFLAAAGGKLKIDG
                     MAAYVQWFNDGVKLVMN"
     gene            2652..4322
                     /locus_tag="PLR47_06505"
     CDS             2652..4322
                     /locus_tag="PLR47_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOG12632.1"
                     /translation="MGMINGGHLVGKYLKEVEKVEIVFGISGGHLESILDSFTEYRIR
                     TIDVRHEQAAAMMAHAWSVYTGKPGVCFLTTGPGFTNGLTGIANACLDNAPMVVICGK
                     HPLRDDLRGALQEMNQLDVVKPITKWAATCYDIKRIPEYLAIAFRQAVEGRPGPVMLE
                     LPPDILNIKVDESLAPMPSRSTRKYTVHPDDADLSAAADLINNAAKPLFIGGTGVGFS
                     SCEDCLGQFIDKTGMPFILLNNGRGTLPDNHPLSVWDCGNVGLMVAIAQADVIVIAGI
                     RLNWLLQSGAVIPPATKVVRIDIDPHEIDRNRQADAGLVGDIGSVLGQLNPLLVKKDR
                     GEWVTLLRAASRSMIDGELKIRQTASDPIHPIRLAEQIQSCVGDDAFYVIDGGDTSYF
                     GLVGFMSRHQAGVISQSSGLLGCLGTGIPFAMAAKLAHPDKKVLLFNGDGSFGFNAME
                     FDTMVRHNIPVVCVVNNDCAWGMIKHTQEMSIGCDRLQCSELGTRHYEKMVEGLGGHG
                     EFAAKDEDIIPALNRAFQSGKPACVNVMTDPTVTSPASVLFYQSLKME"
     gene            4357..5571
                     /locus_tag="PLR47_06510"
     CDS             4357..5571
                     /locus_tag="PLR47_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOG12633.1"
                     /translation="MQALQFNVNVPKFLIIQALRPISASFCYSGPFATVKLVEMPEPK
                     LPAPEWVKIKTRLCGVCGSDMNLIFLKDSPTASPFTSFPCVPGHEICGEIVETGADVS
                     HLKNGDRVVVVPALNCYARAIRPVCRSCAAGTTANCENFAEGAFSPGMFIGICKDING
                     GFAEFFVAHKSQVHLVPDGVSTESAALVEPLSVGLQAVLDNRPRDTDKVLIIGGGVIG
                     AMVVKSMRALDIGCDITVVEPSSFASEHLKACGAHRTIRGGIIDAAVEITGAHAYQPL
                     LGERVLMGGFDKIYDTVGHADTLNLALRVLTTNGTLSVIGIGKEVKLDLTTLWLKLQT
                     VKGCYGYRYNHTANGGKHTYQMALELISSRKIRVDDLLTHKFPLERYREMIEVNLNKE
                     ANRAMKTAVSFE"
     gene            complement(5603..>5846)
                     /locus_tag="PLR47_06515"
     CDS             complement(5603..>5846)
                     /locus_tag="PLR47_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12634.1"
                     /translation="RARKAGHQSPPRVFRNAQWAGGIAALRDPAAEAKGQNEESKRRV
                     NGSNASIPSSAAGEIDRLRRLAPQPFFGFLQIAEFL"
BASE COUNT         1282 a         1563 c         1746 g         1255 t
ORIGIN      
        1 aaatcatttt ccagtgcgtt cagttctatc ggaacttcag caaaatgttt gttcacgaaa
       61 aaatcacgga agaggtccag aaatatttca gaatctacgc cacggaatgc tacgccctgg
      121 gcattcccat ggccgagatg gtctatgcgc tgattttgat gcggcggcac atctggctct
      181 atgccgagtt tcagatgatc ttcagctcgc tgatcaacca gcagcaggcg ctggacaccc
      241 tgaaccgcac cattttgctc ttcgattacg cctcctatga cgtgacccgg gaatatcagg
      301 agctggccaa gagagacaag ctggaatcga tcaggctctt ggatatcctg gaatccaatg
      361 ttgtggaaat tgcctcgaac tgggccgcga ccgtgcggaa ggacaagcag acggtttttt
      421 atcacggcat ccccaaggat aaattgatcc cgcaggcgat caaattttac agccgcctga
      481 ggacccttct tttcgatccc gaacgctttg aaaaaggccg cgaattcttt cggcagtatg
      541 cggagacctg ccgccagcag ggcattcccc tgcacgaggc catttatgcc ctgaacgtca
      601 tgcgccgcca gatgtggctg cacggcgaat ttcagcgaac cttcgtcaac gccctggcgc
      661 atcagcaggc cgtggacagc ctgatgcgca tcatactgct catggactac gcggcgtacg
      721 atatcacgca ttattatcag gagcgaatgc ttctggagga ttgacccgcg ggaggcgcgc
      781 cggggattca aagcgataga cgcgggaaag ggtgagatcg gtcaacggcc gcgggattga
      841 aaagcaacgg cgcggctgct gtgcaaataa aagtttaact tgctatttgc acgggaacga
      901 attatttgaa gcggcatgat tattcgagat atttcagaag aaatggattc gtaatcgccg
      961 cctttttttg aaaatattca aaaacgtgcg atcaagtttc caaaataaat ttcacggatg
     1021 cgggccaggc ggcttttctt ctggatatcg gcacggaact tcaggccgta cgggatccgt
     1081 tgtgcggcaa tctctatgaa aaattcatta gtgcggacat catgaaaggc acgcaaaaca
     1141 aaggaattcg gccggagctg tttcccctcc actcccgcgg caatgacgcc gatttgctca
     1201 cccgggaaaa gggcgcgctg acgccggtcg agataaaatc ttccgccgga agcctgccgg
     1261 tcccggtaca tcggaaagga acgatatgaa tgcccttgtg accggcgcta caggattcat
     1321 aggctcggtt ctggtgagag atttgctggc tcgcggcagt tccgtccggg cgctggtgct
     1381 tcccggggaa aatgccgatg tcttgaaaaa acagggtgtg cgggttattc ggggagacct
     1441 gaccgatccg gaaagtctgc gcggggtctg cgaaggcatt gaaacggttt ttcacctggc
     1501 cgggcgtgtg acggactggg gcacgaagcg gcaattttac acggccatct gtgatgccac
     1561 gggaaacctt ttgcaggaag cggcaggcag ggcatcacgt tttgtttatg tcagctccat
     1621 cgcggccatg ggaatgggcc gtcacctcaa aggcgtgaag gaatccgatc ccccgcggaa
     1681 atccggagtt tcctacaacg acgcgaaagt tgacgcggag gcattggtgc gcgcctttca
     1741 tcgtgaagac aagatgaaat gcaccatcgt gcgccccgcc aatgtggtcg gtccgggaag
     1801 tgtgtgggtg cgggacattg ccgatcgcat gatgtccgcg accggcgtgc ccctcatcga
     1861 tggcgggcgg tacagcgcca gctttgtgta tgtggatcat ctggtggacg gcatgatcct
     1921 ggccgccgcc ggggacattg cctgcgggaa ggcgtatcat tttcgcgacg actggcgcgt
     1981 aaactggaaa gagtacatca cggatctcgg ctcatggatc gggaagcgcc ccaggggaag
     2041 cattcctttc cggctggcgt ggatgaccgg cttcgttttg gaagccgtgc tcaatcccct
     2101 tcatatccgc gctcccctga ccaggctgag cgcgggggtg ctgggcaggg acaatgacgt
     2161 ggataccatg ctggcccgca cggagttagg ctggcgcacg cggttatcct atccggaagc
     2221 catggaaaaa atcggcgcgt gggtgaagga ccattatcaa atcgggagat gatgatgaaa
     2281 ttaagactct tgcttttcat cctgtaccaa aaactgaaga aggccgcggc gaaaaacgcg
     2341 gcataccgaa gttttcttgg cggcatgcag gttaaaatca tgattcaaac ggccgacggg
     2401 aagcacggaa ggctgttcgt ttttgaccgg ggcaagctgt ccaccctgtc cgggtgcggg
     2461 cattcgtatg acgcggcgct tgtctggtcg gattccggca ccgctttcca ggtgatgtcg
     2521 tcgggcagtg atgaacagac ttttctggcg gcggccggcg ggaaactgaa aatagacggc
     2581 atggcagcct atgttcagtg gtttaatgat ggtgtgaaac tggtcatgaa ctgaaaataa
     2641 ggagacggac gatgggaatg atcaatggcg ggcatctggt cgggaaatat ttgaaagagg
     2701 tggaaaaggt cgaaatcgtg ttcggcattt ccggcgggca tctggagtcg attctggaca
     2761 gttttacgga atacaggatt cgcaccattg acgtccggca cgagcaggca gcggcgatga
     2821 tggcgcatgc ctggtctgtt tataccggca aacccggcgt atgttttctc accacggggc
     2881 ccgggtttac caacgggctc accgggattg cgaacgcctg cctggacaat gcgccgatgg
     2941 tggtgatttg cggcaagcac ccgctgaggg acgatctgag gggagctctt caggaaatga
     3001 atcagctgga tgtcgtcaag ccgatcacca aatgggcggc cacctgttac gatatcaagc
     3061 ggatcccgga atatctggcg atcgctttcc ggcaggccgt tgaggggagg cccggtccgg
     3121 tgatgctgga gctgccgccc gatatcctca atatcaaggt tgatgagtcc ctggcgccga
     3181 tgcccagccg gtccacgcgg aaatacaccg tccaccccga cgacgccgac ttgagcgcgg
     3241 cggcggatct gatcaacaat gccgcaaagc cattgtttat cggcggaacc ggcgtggggt
     3301 tcagttcctg tgaggattgt ctcgggcaat tcattgataa aacggggatg ccctttatcc
     3361 tgctcaataa cggccggggg acgcttcccg acaaccatcc cctttccgtc tgggactgcg
     3421 gcaacgtagg cttgatggtc gccattgccc aggcggatgt gatcgtgatt gcgggaattc
     3481 gcctgaactg gcttttgcag tcgggcgccg ttattccgcc cgccaccaag gttgtgcgga
     3541 ttgatatcga cccgcatgaa atcgaccgga accggcaggc cgatgccgga ctggtcggcg
     3601 atatcggttc cgtgctgggg cagctcaatc ccctgctcgt caaaaaggac cgcggtgaat
     3661 gggtgaccct gctgagggcg gcaagccgct ccatgatcga cggagaactg aaaattcgcc
     3721 agacggcatc cgatcccatc catccaatcc gtctggccga gcaaattcaa agctgtgtcg
     3781 gcgatgacgc cttctatgtc attgacggcg gggatacatc ttatttcggc ctggtcgggt
     3841 ttatgtccag gcatcaggcc ggcgtcatca gtcagtcttc gggccttctg ggctgcctcg
     3901 gcaccggcat tccgtttgcc atggccgcca aactggcgca tccggacaag aaggtcctgc
     3961 ttttcaacgg cgacggctct ttcggcttta atgccatgga attcgacacc atggtgcggc
     4021 acaacatccc ggttgtctgc gtggtcaaca acgactgcgc ctggggcatg atcaagcata
     4081 cccaggaaat gtccatcggc tgtgatcggc tccagtgctc ggaactggga acacggcact
     4141 acgaaaaaat ggtcgaagga ttgggcggac acggcgagtt tgccgccaaa gatgaagaca
     4201 ttattcccgc gctcaaccgg gcctttcaat ccggaaaacc ggcctgtgtg aatgtgatga
     4261 ccgatccgac cgtgaccagt ccggcatcgg ttctatttta tcagtcgctg aagatggaat
     4321 agcgcctcaa tcagaacacc ggcaaggagg cctttgatgc aagcgctgca atttaacgtg
     4381 aatgtgccga aatttctgat catccaggcg ctgcggccga tcagcgcaag cttctgctac
     4441 agcggcccct ttgccacggt gaagcttgtc gaaatgccgg agccgaaact tccagcgcct
     4501 gagtgggtca agataaaaac gcggctctgc ggagtttgcg gcagcgacat gaatctgatt
     4561 ttcctgaagg attccccgac ggcatcgccc tttacctcct ttccctgcgt tccgggacac
     4621 gagatctgcg gcgagatcgt ggaaaccggc gcggatgttt cccatttgaa aaacggcgac
     4681 cgggtcgtcg tggtccccgc gctcaactgt tatgcacggg cgatccggcc cgtttgccgg
     4741 agttgcgcgg cggggacaac ggccaattgc gaaaacttcg ctgaaggcgc tttctctccc
     4801 gggatgttca tcggcatctg caaagacatc aacggcggat ttgccgagtt tttcgttgcg
     4861 cacaaaagcc aggtgcacct tgtgcccgac ggcgtttcca cggaatcggc ggccctggtg
     4921 gagccgctgt cggtgggcct gcaggctgtg ctggacaacc ggccgcggga tacggacaag
     4981 gtgctgatca tcggcggcgg cgtcatcggc gccatggtgg ttaagagcat gcgggcgctg
     5041 gatatcggct gcgatattac ggtggtcgaa ccgtcttctt ttgcctcgga gcatttgaag
     5101 gcctgcggcg cccatcggac catccggggc ggaatcatcg acgccgccgt ggaaatcacg
     5161 ggcgcccatg cctatcagcc gctgctggga gagcgcgtcc tcatgggagg cttcgacaag
     5221 atttatgaca ccgtgggtca tgccgacacc ctgaatttgg cgctgcgcgt cctgacgaca
     5281 aacggcacgc tttccgtcat cggcatcggc aaggaagtca aactggacct gaccacattg
     5341 tggctcaagc ttcagaccgt caaaggttgc tatggctacc ggtacaatca tacggcgaat
     5401 ggcggaaagc atacgtatca aatggcgctg gaattaattt catcccggaa aatacgcgtg
     5461 gatgatctgc tgactcacaa gtttccgctt gagcgatacc gcgaaatgat agaggtcaac
     5521 ttgaacaaag aggccaaccg cgccatgaaa accgctgtca gctttgagta gggatttgcc
     5581 gagactgcgg atggcgcgac aattacaaaa actctgcaat ttgcaaaaag ccgaaaaagg
     5641 gttgtggcgc taaacgccgc aaccggtcga tttcaccggc ggcggaggac ggaatcgatg
     5701 cgttgcttcc gttcacccgc cgtttgcttt cttcattctg acctttcgct tcagcggctg
     5761 ggtcgcgcag cgcagcgatc ccccctgccc attgtgcgtt ccgaaaaaca cgcggtggac
     5821 tctgatgccc cgcttttcga gctcgg
//
LOCUS       DAOSIM010000126         5763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_12566_length_5763_cov_6.175893, whole genome shotgun sequence.
ACCESSION   DAOSIM010000126 DAOSIM010000000
VERSION     DAOSIM010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12566_length_5763_cov_6.175893"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLR47_06520"
     CDS             complement(<1..217)
                     /locus_tag="PLR47_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG12635.1"
                     /translation="MASILVIDDDASIAETLDLYLTEEGYSVRTALTGTDGLNKYVQE
                     PSDVVILDIRLPDVDGFSVLEDLKEENE"
     gene            complement(292..2862)
                     /locus_tag="PLR47_06525"
     CDS             complement(292..2862)
                     /locus_tag="PLR47_06525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG12636.1"
                     /translation="MALLRNSYRFLRQAFSLRIWLTSLIVILLILLLALTVNNAREKE
                     MADLFSRQQLASAQNASARMTEILSQVERNVALFSYFDPNGKILSPKDHREIEILYSV
                     WGKTIDAVLVFDAREGVRRVLSQEALPRVNLIPHFRILKNQKRQYLSLALSDVRAGGA
                     PASRQDWYLIWGNPIWRDRNIFDGAWMVSFSLAALVHAGERQIQENRLGDLWLIDDRG
                     RIVLHPNSPYIGKNTGDLVRDSRGNAITLSPAGGRLIDARVLRQSGALERSVIAYYPF
                     QVAGNSWSVMVSAPYAEVVAPVRTTFLYTMFSAFLLMLILVVAGIFYAFREGRRLRMR
                     EVQERLKERETWQEKLLREKKTIEGIIEGSPIPSFVINKQHQVILWNRACVELTGQSA
                     EDTLGTDGQYRAFYPVQRPMVADLIIDSDIDALNKYYSAKKVKKSGKVLGAYEAYDYY
                     ENLGGRSRYMYFLAAPIYDEDGNIIAAIETLQDISREQELTRSLQEYAETLQNELSEN
                     IELRKEIEGLLNYMQSIVKSLPDKIYEVDKDGVINFISWGLKKPKNEFRGRHFLEFVA
                     PENADFVVAKWEDAKRGNYSPYEIETVAQDGRKVNLMVTTSPVIGSDHYILVQRDITE
                     FKNLEKKLYDSQKLAALGQLSAGIAHEIRNPLSSIKMSLQILEKRIKPEGNDLKRFKI
                     AQKEVEHLEELVNNVLVFAKPMEPKIESVDLAKVLDQAAALSEHIILDKNIDVTRDTR
                     DIPAVNVDAAMITDAFVNIIRNAVEAMEDGGHIHIYARSIGSRPPSVLVVVEDDGCGI
                     DDADMPHLYNPFFTKKKYGTGLGLSQVLKIVEVHQGKLEIISEKGKGTKVCVTLPCSK
                     "
     gene            complement(2869..3042)
                     /locus_tag="PLR47_06530"
     CDS             complement(2869..3042)
                     /locus_tag="PLR47_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12637.1"
                     /translation="MQELLTVVTYPIIWLVCSYLLKIESGLAKLLISLTAAVLVYVLY
                     GLWQSQKEKQKEN"
     gene            3539..3916
                     /gene="atpE"
                     /locus_tag="PLR47_06535"
     CDS             3539..3916
                     /gene="atpE"
                     /locus_tag="PLR47_06535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOG12638.1"
                     /translation="MSHKHGKTILTVFTSVAALLLSSQFAFAADAIPVGGESYIKVIF
                     VVGAMIGAGLAIGLGAIGAGAGIGNAANGACQAVGRNPGVQGKIMMVMLVGMAMAESI
                     AIYALVIALILLYANPFKPFFLG"
     gene            4187..5281
                     /locus_tag="PLR47_06540"
     CDS             4187..5281
                     /locus_tag="PLR47_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12639.1"
                     /translation="MESKKDLQVATIIPSTESVYKQFIAENKAPLWVKKDGKVQQVLT
                     PLYKEMSLIDKNGNERIKIADGQAVPAGKLVDVSKPANTTYKNEDYFVRTKNLNRGEV
                     YVSPVTGWYVNKAAFDKGERFNGVVRFATPVFNQEGFSGIIVLSLDYRHLGQITDQLI
                     PTQAERVFETEASSGNYAYMFDYRGNAIAHPNDYHIVGLNPDGTPVPIITQQNVKELT
                     DKGADALNIIQAGFLDPNLTALAKETAEGKTGIKAYKLFGMTKFVAYAPIKFYASNIP
                     EPAGFGWVCLGVEVEKYNEAALKVSQNIEKESKAWTATVILVLIVAMVILFFIMLILV
                     RGISRSIQSEVPEGSEGDLSGLNDDDDEDK"
     gene            5398..5607
                     /locus_tag="PLR47_06545"
     CDS             5398..5607
                     /locus_tag="PLR47_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12640.1"
                     /translation="MDAFLKLFPFLHLVVLAAGGILLFAVKRKYPKVRNAELITVFIL
                     FLILVALFTETGLDWIKRLMSLIQV"
BASE COUNT         1343 a         1552 c         1469 g         1399 t
ORIGIN      
        1 tttcattttc ttccttgagg tcctccagga cggaaaaccc gtccacgtcc ggcagacgga
       61 tatccagaat gacgacgtcc gaaggctcct gaacgtattt gttcaggccg tcggtgccgg
      121 tcagcgccgt acgaacgctg tatccctctt cggttaaata caaatccagc gtttccgcaa
      181 tggacgcatc gtcatcaatc accagaatac ttgccatagt cataaccttc gttgcctgcc
      241 ccgcggcgcg ctttctttcg gggtttcagt cccctcttcg aaaacgatcc gttatttact
      301 gcagggtaat gtcacgcaaa ctttcgtgcc cttgcctttc tcgctgataa tctccagctt
      361 cccctggtgc acctccacga tcttcaggac ctgcgacagg ccgaggcccg tcccgtattt
      421 cttcttggtg aaaaacggat tatacagatg gggcatgtcc gcatcgtcga tgccgcagcc
      481 gtcgtcttcc acgaccacca gaacggaagg cggccgggat ccgatgctgc gggcgtaaat
      541 atgaatgtgg ccgccatcct ccatcgcctc caccgcattg cggatgatgt tgacgaaagc
      601 gtccgtaatc attgccgcat ccacgttcac cgcgggaata tcacgggtgt cccgggtaac
      661 atcaatgttt ttatccagaa tgatgtgctc ggagagagcc gccgcctggt ccaggacttt
      721 cgcaagatcg accgattcga ttttgggctc catcggtttg gcaaacacca gaacgttgtt
      781 caccagctct tcgaggtgct cgacttcctt ttgcgcgatt ttgaagcgtt tcagatcgtt
      841 gccttcgggc ttgatgcgtt tttccagaat ctggaggctc atcttgatgg acgagagcgg
      901 attgcggatt tcatgggcaa tgccggcgga aagctgcccc agcgccgcga gcttctggct
      961 gtcgtaaagc ttcttttcca ggttcttgaa ctcggtaatg tctctctgca ccaggatgta
     1021 atggtcgctc ccgatcaccg gggacgtcgt caccattaaa ttaaccttgc gcccgtcctg
     1081 ggccaccgtt tcaatttcat agggggagta gtttccccgc ttggcgtctt cccatttggc
     1141 aacgacaaaa tcggcattct ccggcgcgac aaattccaga aaatgccttc cgcgaaattc
     1201 atttttgggt ttcttcagcc cccaactgat aaagttgatg acgccgtcct tgtccacctc
     1261 atagatttta tccggcaggc ttttgacaat ggactgcatg taattcagca ggccttcgat
     1321 ctctttgcgg agttcgatgt tttccgagag ttcgttctgc agcgtctcgg cgtattcctg
     1381 caggctgcgc gtcagctcct gctcgcggga gatgtcctgc agcgtttcaa tcgccgcgat
     1441 gatgttccca tcctcgtcgt aaatcggcgc cgcgaggaaa tacatgtacc ggctgcggcc
     1501 gccgaggttt tcgtaataat cataggcctc ataggcgccc agcacctttc ccgatttttt
     1561 gacttttttg gcgctgtagt atttgttgag cgcgtcgata tcgctgtcga tgatcaagtc
     1621 ggccaccatg ggcctttgga cgggataaaa cgcccggtac tggccgtccg ttcccagcgt
     1681 gtcttccgcc gactgcccgg tcagctccac gcaggccctg ttccacagaa tcacctgatg
     1741 ctgtttgttg atcacgaagc tgggaatggg cgatccttcg atgatgcctt caatcgtttt
     1801 cttttcccga agcagtttct cctgccaggt ctcacgctcc ttcagccgct cctgcacttc
     1861 ccgcatccgc aatcgccttc cctcccggaa cgcgtaaaag atgccggcga ccaccaggat
     1921 cagcatcagc agaaatgcgc tgaacatcgt gtaaagaaaa gtcgtgcgca cgggagcgac
     1981 gacctcggcg tagggagcgc tcaccatgac ggaccagctg ttcccggcaa cctgaaacgg
     2041 atagtaggca ataacgcttc tttccaaagc gccgctttgc cgtaaaaccc tcgcgtcaat
     2101 gagtcttcct cctgccggcg aaagggtgat tgcgtttccc cggctgtccc ggactagatc
     2161 accggtgttc ttgccgatgt agggcgaatt cggatgcagg acaatccgcc cccggtcatc
     2221 gatcagccaa aggtctccca gccggttttc ctgaatttgc ctttctccgg catggaccag
     2281 cgcggccagt gaaaaggaca ccatccacgc gccgtcaaaa atattgcggt cgcgccagat
     2341 gggattcccc catattaaat accaatcctg tctggaagcc ggggcgccgc ctgcgcgaac
     2401 atcggacagg gccagactta aatattgccg tttctgattt ttcaggatcc ggaaatgggg
     2461 gatcaggttg acgcggggca gcgcctcctg cgacagaacg cgccggacgc cctcccgggc
     2521 gtcaaacacc agcacggcgt cgatcgtttt tccccaaacg gaataaagga tttcgatttc
     2581 ccggtgatct ttcggcgaca gaatttttcc gttgggatca aagtaggaga acagagcgac
     2641 gtttctttca acctgcgaaa gaatttccgt catgcgggcg gaggcgtttt gagcggacgc
     2701 caactgctgg cggctgaaca aatccgccat ttccttttcc cgcgcgttgt tcacggtcag
     2761 agccaacaga aggatcagca gaataacaat gaggctggtc aaccagatgc gcagactgaa
     2821 cgcctggcgc agaaatcggt atgagtttct cagcaatgcc actgaatctt aattttcctt
     2881 ttgtttttct ttttgagact gccacaaacc gtacaaaaca taaaccagaa ccgccgccgt
     2941 gagcgagatc agcagttttg ccaggccgct ttcaattttt aaaaggtaag aacacaccag
     3001 ccagataatc gggtacgtaa ctacggttaa aagctcctgc ataacgatcc tttgtcattc
     3061 gtaatgatgt cccatgattt ggcacaggtt tgggcatcgt aacgacattg atccaaaatt
     3121 tcaactgaaa tatcagaaaa cgtttttcac cccgatttgc aaaaggcgtc tctcccttca
     3181 cgctacagcc cgtttaatgt tttatggcag gccattgaac aaaacattaa acgacgcgct
     3241 gaaacgcacc ctcttgcgcg acccgccgga aaaccaaagt cattcccccc tgcgttcttt
     3301 cgcgccggcg ccgaacccaa acgaacagaa atttcaatgc attgaacagc ccgccgaaac
     3361 ggacgggcgc cgcccgcgcc gcggcaaaaa acaaattttc tcaattgaaa ccatggcaca
     3421 tctcatgcat tagcgtcgag ccgattgatg ttaattcttc tttgcgcagc aatgattaat
     3481 gtgttatgca gcgcaaaaaa gaaacaaata aaccatttgt catgaaagga gtcagataat
     3541 gtcacacaaa cacggaaaaa caattttaac ggtcttcaca tcggtagcgg ctctgctgct
     3601 ttcgagccag tttgccttcg cggcggatgc gattccggtg ggcggagaat cctacattaa
     3661 agtgatcttt gtcgtcggcg ccatgatcgg cgcgggtctt gccatcggtc ttggcgccat
     3721 cggcgcgggc gcgggcatcg gcaacgcggc aaacggcgcc tgccaggcag tgggaagaaa
     3781 ccccggcgtt cagggcaaaa tcatgatggt gatgctggtc ggcatggcca tggccgaatc
     3841 gatcgccatt tacgctctgg ttatcgcact gatcttattg tacgccaacc ccttcaagcc
     3901 tttcttcttg ggttaatgct attgagaagt tcactacaaa attaaccaag gttaggggga
     3961 aattataatg tcacaatcta aagataagaa gtcattacct gttgcaggcg tattcttcct
     4021 gcttattgta atccccttat cactgatggc gtttttaatc gctaacggca tgtttaaact
     4081 gggggttacc ataaaagaaa gggccgttaa cgttctggat gtcaaatccc aggaagatat
     4141 taaagcaagg gccatcaaca ctgcaaatca ggttgccaac ttactcatgg aaagcaagaa
     4201 ggatttgcag gtcgcaacca ttattccctc gacagaatcc gtctacaagc agtttattgc
     4261 tgaaaataaa gcaccgctct gggtcaagaa agacggcaag gtccagcagg tgctgacacc
     4321 gctgtacaag gaaatgtccc tgatcgacaa aaacggcaac gaaagaatta aaattgccga
     4381 cggtcaggcg gtgccggcgg gaaaactggt tgacgtgagc aagccggcca ataccaccta
     4441 taaaaatgaa gattatttcg tccgaacaaa aaatctgaac cggggcgaag tgtacgtttc
     4501 tccggttacc ggctggtatg tgaataaagc ggccttcgat aagggagaac gattcaacgg
     4561 tgttgtccgt tttgcaacac cggttttcaa tcaggaaggt ttttccggga tcatcgtgct
     4621 gtccctggac taccgtcacc tgggccagat caccgaccag ctcattccca cgcaggcgga
     4681 gcgggtgttt gaaacggaag catcctccgg caattacgcg tacatgttcg attaccgcgg
     4741 caacgcgatc gcgcatccga acgattacca tatcgtaggc ctgaatccgg acggaacgcc
     4801 ggttccgatc atcacgcagc aaaacgtgaa agaactgacg gataagggcg ccgatgcctt
     4861 gaacattatt caggccggtt tcctggatcc caatttaacg gctctcgcga aagagacggc
     4921 ggaaggcaaa acggggatca aggcgtataa attattcggg atgaccaagt ttgtcgccta
     4981 tgcgccgatc aagttttacg cgtcaaacat tcctgagccc gccggttttg gatgggtctg
     5041 tctgggggtc gaagtcgaga agtacaacga agcggccctg aaggtctctc agaacatcga
     5101 gaaggaatcc aaggcatgga cggcaacggt cattctggtg ctcatcgtgg ccatggtgat
     5161 cctgttcttc atcatgctga ttctggttcg cggaatttcc cgctccattc agtcggaagt
     5221 tccggaaggt tccgaagggg atttgtcggg gctgaatgat gacgacgatg aagacaaata
     5281 agcagcgttg attcatgaaa aaaaaggccg aatttcgatt cggccttttt tattgatgtc
     5341 ggcctggatg aattttccgg aagaggtttt ataaatccgg tccacaagga gattcgaatg
     5401 gatgcctttt taaagctttt cccctttctg cacctggtgg tactggcggc gggcggcatt
     5461 ctcctgtttg ccgtaaaaag aaaatatccc aaagtccgca atgccgagtt gattacggtc
     5521 ttcattctct ttttgattct ggtcgcactc tttacggaga cggggctgga ttggatcaaa
     5581 cggctgatga gccttatcca ggtctagccg ccgtgagcag gcgcttcgcc tttatcacgg
     5641 ggcggttcca gtcagaacag gcaggcacgg agaaaatcgt aagccgagtt ctgttccgcg
     5701 gtccggttgc cctcgccgcg gcgatgatca ttcatctggg acggcagttg cctgccgcct
     5761 cta
//
LOCUS       DAOSIM010000127         5739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_12644_length_5739_cov_6.946517, whole genome shotgun sequence.
ACCESSION   DAOSIM010000127 DAOSIM010000000
VERSION     DAOSIM010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12644_length_5739_cov_6.946517"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="PLR47_06550"
     CDS             complement(<1..1233)
                     /locus_tag="PLR47_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOG12641.1"
                     /translation="MAPKSKISRTRNIGIVAHIDAGKTTVSERILFYTGKSYKMGEVH
                     DGEAIMDWMPQEQERGITITSAVTTCAWKNSEIHIIDTPGHVDFTIEVERSLRVLDGA
                     VVVFCAVAGVEPQSETVWHQADKYGVPKIAFVNKMDRVGADFFHVVEMMRDRFGSLPV
                     PVQIPVGSEDSFRGVVDLINRKLLIWNESSQGTDFTVSEVPDHLAEDVSREREKMIET
                     LANCDDDLAEIYLAGQDATAAQIIAALRKATIALKTVPVLCGAALRNKGIQPILDAVI
                     HYLPSPEDIPPVQGINPKTKQQEVRHSSDKEPFSALAFKVQLDEGRKLTYLRVYSGQI
                     AAGGEIYNASKKKKEKIARLLQMHSNKRERIERASAGELVAVLGLKETTTGDTLCDEA
                     RPIILEQMEFYEPVISQAI"
     gene            complement(1241..1873)
                     /locus_tag="PLR47_06555"
     CDS             complement(1241..1873)
                     /locus_tag="PLR47_06555"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOG12642.1"
                     /translation="MSTKKQRRSENFKDRQKKKSTRIAYLAAIVGGSVLLLVIFFVLL
                     FNALFPPVDMEALNRKKISVRIYFADPQERFLVPEKRVIYKEEDPVLQAKEVVNALLD
                     GSKTKSKTGNVNTFPARVILKDIGLDKDGTAEVSFSSDLTKNHPGGSTAELMTIYSLT
                     NTLTEIVPQIRAVRILVDGKELSSIKGHISTAGPFLPDPDLYAPAKEEKS"
     gene            complement(2073..2651)
                     /locus_tag="PLR47_06560"
     CDS             complement(2073..2651)
                     /locus_tag="PLR47_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOG12643.1"
                     /translation="MAKSIKGTQTEKNLLASFAGESQARNRYTFFASAAKKEGYEQIS
                     RFFLETAENEKEHAKVFFKYLEGGELEITASYPAGVIGDTKSNLEAAAAGENMEWTVL
                     YADAAKIAKEEGFPEVARSFEQIAKVEKFHEERYRKLAANIAKGEAFAKKEAVKWHCI
                     NCGYVYEGPEAPKECPACKHPQSYYEVLAENY"
     gene            2998..3180
                     /locus_tag="PLR47_06565"
     CDS             2998..3180
                     /locus_tag="PLR47_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12644.1"
                     /translation="MATIMPEAENVQKAIKWISGCLEDGEAQPVHMLIEKAVFKFDLS
                     PKDTEFLMGFFRNHKK"
     gene            complement(3379..3771)
                     /gene="mscL"
                     /locus_tag="PLR47_06570"
     CDS             complement(3379..3771)
                     /gene="mscL"
                     /locus_tag="PLR47_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOG12645.1"
                     /translation="MASRKVSAMVEEFKNFALKGNVVDLAIGVIIGAAFGKIIDSLVK
                     HIIMPLISLVMPGEQGYLAWKWVVNGKELPYGLFIGEVVNFLIIALALYIFIVKFIGM
                     IVKNKEEAPPAPTKDQELLTEIRDLLKK"
     gene            complement(3794..4231)
                     /locus_tag="PLR47_06575"
     CDS             complement(3794..4231)
                     /locus_tag="PLR47_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12646.1"
                     /translation="MIHKAPGICFFLIAVFFLASCAGKFGSEVLSSPDEYRHVYEAKE
                     KYILKAIAGVLEEKKIGHNVVIDYANYRVDSDYVVSGDWRTKTSARVRRLNWKECEVT
                     LIVTTEKKTDNGWEMRRLLKKEQYDNFFSVIELKTYEEMSRIY"
     gene            complement(4256..4834)
                     /locus_tag="PLR47_06580"
     CDS             complement(4256..4834)
                     /locus_tag="PLR47_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG12647.1"
                     /translation="MESIADFLFEAGMLSKTPRSGYQFLGSGRESVAEHILRTVFIGY
                     ALCKLNPSLNELRVLKMCILHDLPEARTGDMNYVNKKYVEVHEAKAVKELTESLFFGE
                     DIRQAIDEFNARETPEANTARDADQIALILQLKEYGDLGNKYSDEWIHYALRRLATAD
                     GKKLADRIIQTDSSHWWFKDKSDWWVNGSRRG"
     gene            complement(4836..5054)
                     /locus_tag="PLR47_06585"
     CDS             complement(4836..5054)
                     /locus_tag="PLR47_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12648.1"
                     /translation="MAPMDPSTAFMKEYQERFEKKLKENEMALLEHWKSQLEKIESSR
                     PDSIASLQLQIRKVSEMMANRIKVLKKG"
     gene            5206..>5739
                     /locus_tag="PLR47_06590"
     CDS             5206..>5739
                     /locus_tag="PLR47_06590"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOG12649.1"
                     /translation="MGCKPKPEDIAGGQAILEGVMMRHGEKIAAAVRRPDREIVFQER
                     NYVPLTKRYKPLGWMFIRGTVSLFEMMIVGIQSLMFSAEVALSEEEKKPQGWEMYVSF
                     AISFAVAIFFFVVVPAFFFTRVKEYFDSLILLNILEGCVRLGIFLCFLSATLLMADMR
                     RVYMYHGAEHKTVFAWED"
BASE COUNT         1193 a         1584 c         1457 g         1505 t
ORIGIN      
        1 gatggcctgg ctgatgaccg gctcgtaaaa ctccatctgc tccagaatga tcggccgcgc
       61 ctcatcgcac agcgtgtcgc cggttgtcgt ttccttcagc cccagaacgg caacgagttc
      121 gcccgccgat gcgcgctcga ttctttcccg cttgttcgaa tgcatctgca gaagccgcgc
      181 gattttttcc ttcttcttct tggaagcgtt gtagatctcg ccgccggctg cgatctgtcc
      241 ggaataaacg cgcagatacg tcagcttgcg cccttcgtcc agctgaactt tgaacgccag
      301 ggccgaaaaa ggctccttgt cggagctgtg gcgaacctcc tgctgtttgg ttttcgggtt
      361 gattccctgg accggcggaa tgtcctccgg cgacggcaga tagtggatga ccgcgtccag
      421 aatgggctga atgcctttgt tgcgcagggc ggctccgcac agcacgggca cggttttcag
      481 ggcgatggtc gccttgcgaa gcgccgcgat gatctgcgcc gccgtcgcgt cctggccggc
      541 caggtaaatt tccgccaggt cgtcgtcgca gttggccagg gtttcgatca ttttctcccg
      601 ctcgcgtgat acgtcttcgg ccaggtggtc cgggacctcg cttacggtaa agtccgtccc
      661 ctgcgacgat tcgttccaaa tcagaagttt gcggttgatc aggtcaacca cgccccgaaa
      721 cgaatcttcg cttcccaccg gaatctgcac ggggacggga agagatccga agcggtcgcg
      781 catcatttcc acgacatgaa aaaaatccgc gccgacccgg tccattttgt tgacgaaggc
      841 gattttggga acgccgtatt tgtccgcctg atgccagacg gtttcggatt ggggctcgac
      901 gccggccacc gcacaaaaca ccaccaccgc gccgtccaga acgcgcaggg accgctccac
      961 ttcaatggta aaatccacgt gccccggcgt gtcaatgatg tgaatctcgg aattcttcca
     1021 ggcgcaggtg gtcacggcgg acgtaatggt gatgcccctc tcctgctcct gcggcatcca
     1081 gtccatgatt gcttcaccgt catgcacctc acccatttta taggacttgc ccgtataaaa
     1141 aaggatccgc tcgctgacgg tcgttttgcc cgcgtcgatg tgggcgacga tgccgatgtt
     1201 gcgcgtacgg gatattttgg atttcggcgc catgggaatg tcagcttttc tcctctttag
     1261 cgggcgcgta gagatcggga tccggaagga aaggaccggc cgtggaaata tgccctttga
     1321 tcgatgacag ttctttgccg tccaccagaa tccggacggc ccggatctgg ggaacaattt
     1381 ccgtaagcgt gttggtgaga gaatagatgg tcatcaactc cgccgtggag ccgccaggat
     1441 ggttttttgt caaatcggag ctgaagctga cctctgcggt tccgtctttg tccagcccga
     1501 tgtctttgag gatcacgcgg gctggaaagg tgttgacgtt gccggttttg cttttggttt
     1561 tggagccgtc cagaagcgcg ttcaccactt cctttgcctg gagcaccggg tcttcttcct
     1621 tgtaaatcac ccgcttttcg ggaacgagaa aacgctcctg cgggtctgca aaatagattc
     1681 ggaccgatat ttttttccgg tttaaggcct ccatgtcaac cggcggaaat aaagcgttga
     1741 ataaaaggac gaagaagatc accagcagca aaacgctccc gccgacgatg gcggccagat
     1801 aggcgatgcg ggtggatttc tttttttggc gatctttgaa attctcggac cggcgctgct
     1861 ttttcgtgga catgacgaca accttctttc ttgaaaaaac tgcgccaatc tagggtattt
     1921 ttccttcagt gtcaagccct tcgataggcc gtgcgatagg ccgccggaac ggcatcccga
     1981 tgaaaattgc aaagctaggc caaacgcaaa aaaaatagcc cgccgaactg aaaaggcggg
     2041 ctatcgcgat atccgtcctg gagatggccg gtttaataat tttcggccag cacctcgtaa
     2101 taggactgcg ggtgtttgca ggcggggcat tctttcggcg cctcgggccc ttcatacacg
     2161 tagccgcagt tgatgcagtg ccactttact gcctcctttt tcgcgaatgc ttctcccttt
     2221 gcgatgttgg cggcgagttt gcggtagcgt tcttcatgga atttttccac cttggcgatc
     2281 tgctcgaagg agcgggccac ttccggaaaa ccctcctctt ttgcgatttt cgcggcatcg
     2341 gcgtacagca cggtccactc catgttttca ccggccgccg cggcctccag attgcttttg
     2401 gtgtcgccga ttacgccggc cgggtaagaa gcggtgattt caagctcgcc gccttccagg
     2461 tacttgaaaa atacttttgc atgctccttt tcattttccg ccgtttccag gaaaaaacgt
     2521 gaaatctgct cgtatccttc tttcttcgcg gcgcttgcaa aaaatgtgta gcggtttctg
     2581 gcctgcgact cgccggcaaa agaggccagc aggtttttct ccgtttgggt tcctttaatt
     2641 gatttcgcca ttctgcatct cctttcattc atttttttga atttgtatat gttttccggt
     2701 gtgttatcaa cccttattcg atctattttg caccctgccg cgaatcatca agatggccgc
     2761 agcgcttgtc cgcctctctc cccaccttcg ttatcgactg ccctggagct ccgccgcgga
     2821 tgatttgctc ccgtactgcc gtcaagttaa tcataaaatt tttaatgtca aacgagccaa
     2881 atattagtta atttttctga cgtattgtag tatgtagctg aaaagttttt gcgctttgtc
     2941 gatgaaatcc tgaccgcgga aaactgctta aaacattacg aaggaggatg aaacaatatg
     3001 gcaacgatta tgcccgaagc tgaaaatgtt cagaaggcga ttaaatggat ctccggctgt
     3061 ctggaagacg gtgaagccca gccggttcac atgctgatcg aaaaggcggt ttttaagttt
     3121 gatctgtccc ccaaggatac agaattcctg atgggtttct tccgcaatca caaaaaatag
     3181 aagccgccgg gggcgcaaga catgcgtcac gcccccggcg ctgatcccgc aataacctta
     3241 gaaaaccggc cgcgggtctt caggctcaag gctggaaccg cattttccct ccgttttttc
     3301 aaggagggca gacttaaacc gaccgccgaa gattattttt acggccggtc cggcggtcgg
     3361 gacacttccg gacaaccgtt actttttcag cagatcccgg atctccgtca gcagttcctg
     3421 gtctttggtc ggggccggcg gcgcctcttc cttgtttttc acgatcatgc cgataaattt
     3481 cacgataaag atatacaagg ccagcgcaat gatcagaaag ttgaccactt ccccgatgaa
     3541 gagtccgtag ggaagttcct tgccgttgac cacccacttc caggccagat acccctgctc
     3601 gccgggcatg accaggctga tcagcggcat gatgatgtgc ttgaccaggg aatcaatgat
     3661 cttgccgaac gcggccccga tgatcacgcc gatggccaga tccaccacgt tgccctttaa
     3721 agcgaaattt ttgaactctt ccaccatagc cgatactttc ctggatgcca tacattctcc
     3781 tttcgtcttt tcgttaatag atcctggaca tttcctcgta cgtcttcagt tcgatgacgc
     3841 tgaaaaaatt gtcgtactgt tcctttttca gcagacgccg catttcccat ccgttgtccg
     3901 tttttttctc cgtcgtcacg atcagggtca cttcgcattc cttccagttg agtcttctga
     3961 cccgggcgct ggtcttcgtc cgccagtctc ccgaaaccac atagtcagaa tcaacccggt
     4021 agttggcgta atcgatgaca acgttgtgtc cgattttctt ttcttccagg acgccggcaa
     4081 tggctttcag aatgtatttt tcctttgcct cgtaaacatg cctgtattca tcggggctgg
     4141 acaaaacctc cgatccgaat ttcccggcgc aggacgcaag gaaaaaaacc gctatcagga
     4201 aaaagcatat ccccggcgct ttatgaatca tctccgctcc ttttcggaca tttttctatc
     4261 cccgcctgct tccgtttacc caccagtcgc ttttgtcctt gaaccaccag tgcgatgaat
     4321 ccgtttgaat gatgcggtcc gccagctttt tgccgtcggc cgtggcaagc cttcgcagcg
     4381 cgtaatgaat ccactcgtcg gaatatttat tgcccaaatc cccgtattcc ttgagctgaa
     4441 gaatgagcgc gatctgatcg gcgtcgcggg cggtattggc ttccggggtt tccctcgcat
     4501 tgaactcatc gatcgcctga cggatatctt cgccgaaaaa aaggctctcc gtcaattcct
     4561 ttacggcttt ggcctcatgg acttccacgt atttcttgtt gacatagttc atgtcgccgg
     4621 tgcgggcttc gggcaaatcg tgaaggatgc acattttgag gacgcgcagc tcgttcaggg
     4681 acggattgag cttgcacagg gcgtacccga taaaaacggt ccgcaaaatg tgctccgcca
     4741 ccgactccct gccgctgccc agaaactgat aaccgctgcg gggcgtcttg ctgagcatcc
     4801 ccgcttcaaa taaaaaatcg gcaatgcttt ccatcttatc cctttttcag cactttgatc
     4861 cggttggcca tcatttcgga cacctttcgg atttgcagtt gaagggaagc gatgctgtcc
     4921 ggtcttgaac tttctatttt ttcaagctgg gatttccagt gttccagcaa ggccatttca
     4981 ttttccttca gtttcttttc gaaccgctcc tggtattcct tcatgaaggc ggtcgacgga
     5041 tccatgggcg ccatgcgcat cctccctgtt ttcctgaaat tacggcaaga ctctataaca
     5101 ttcatcctga aagatgcaac aaacttttct gcaggcatca tttgacagcc ccggttaaat
     5161 gctataagga aacgctgcaa aaataagcga caggaaacaa aagtcatggg ctgcaaacca
     5221 aaacctgaag acattgccgg cggacaggcc attctcgaag gcgtcatgat gcgccacggc
     5281 gaaaaaatcg ccgccgccgt ccggcgtccc gaccgggaga ttgtttttca ggagcgtaat
     5341 tacgtaccgc tgaccaagag atacaagccc ctgggatgga tgtttatccg gggaacggtc
     5401 agccttttcg aaatgatgat cgtcggcatt caaagcctga tgttttccgc ggaggtcgct
     5461 ctttccgagg aagagaaaaa gccgcagggc tgggaaatgt acgtttcctt cgccatcagc
     5521 ttcgccgtcg ccattttttt ctttgtggtg gtgccggctt tcttctttac ccgggtgaaa
     5581 gagtactttg acagcctgat tcttctcaat attctggagg gctgcgtccg cctgggaatt
     5641 tttttgtgtt ttctgtccgc cacgcttctg atggcggaca tgcggcgcgt ttacatgtat
     5701 cacggcgccg agcataaaac cgttttcgcg tgggaagac
//
LOCUS       DAOSIM010000128         5633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_12920_length_5633_cov_9.430082, whole genome shotgun sequence.
ACCESSION   DAOSIM010000128 DAOSIM010000000
VERSION     DAOSIM010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12920_length_5633_cov_9.430082"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..5286
                     /locus_tag="PLR47_06595"
     CDS             37..5286
                     /locus_tag="PLR47_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOG12650.1"
                     /translation="MMRTAGRQPRILLTNVKQLELLLTRQRDVELFADARLDYLVFDE
                     AHTFAGAQGAETACLIRRLRAFCSRDARDTVCIATSATIADQRNPNAAGDFAGRFFGV
                     DKASVVTVSEAYEQEVWAPKRKTPPLPRKDTGEILNACVSAVEEVNGDGATLRAVYRD
                     LASADLPTGNGPEVLYDVLSQNELVFQLSESLALPRPLHELPEMIGERIGRSVNEAEI
                     LSWLTLGAAARKEGRPLVRPVVHGFIRGISGAVVTFPESEAGPKLWLAAEDEIEAKGE
                     KEQNAHFPVTTCTTCGQHYFITYLKDFDFSGRRPGGGEAEGDGSFWLPLEESQGGKRV
                     VLLDRIVGGSDDEDLDDNERISALYFCRRCGAAHPTSVSRCCHCGMTGVPVVLYAVRQ
                     KKDNPGFLSSCLSCGANGRVIGTTYREPARPVRATNVADVHVLAQDMVHNSERPRLLV
                     FCDNRQDAAFQAGWMKDHARRFRLRALMADGLKGGAMSIGDLAMHLDAILEKDESLSR
                     ALVPEVWQVVRREGSGGRHEQERRKFLRIQVLREVTLSARQAIGLEPWGRMKVDYEGL
                     DPALPWIQENAHALGMPPEDLRDGVAGLLDYLRRKRVLFDPEREVFTHYWMDGDLEIQ
                     QGYLPQLGAPVGTKFHREADEKQELVAQWFSERGDTTIRQMARKWGVPSENAQGFLEG
                     LFRFLVDKRLLVPVRLKGSRGNPLPNVSGVYQLNADVLRLQQNHGVYRCRNCRRRTTR
                     RSPNMLCPAWRCDGSLEFVREDPDNYDLQLLDQGYSMLRPEEHTAMVPHDERERLENL
                     FKGDSEAVNAFVCTPTLELGVDIGKLDAILMRNVPPLPANYWQRAGRAGRRHRMAVDM
                     TYCRPVSHDRAYFAEPLKLLAGQVDPPAFNLRNELMVAKHVHAAVITRLHQFMRDSSR
                     TPTERQDIEDTLKKCLPDQVSAYLFDGGRVRDRLADLTPLRRLIEQNVDDLCEYVERV
                     FRQGWPTADADVTKPQALRDHVQGMTDSLLDVLARLERRLLWAMEQIRRLNGVRERQG
                     DLEPEDEALFRRCDALVKRLKGTARRTRREAEGYDDVNTFGVLAAEGFLPGYGLEVGS
                     ILGTAEIPFWRTGAMAFVLPRPPGFALREYVPGNLIYANGNRFVARRFHRDIDEQKAE
                     MPVFEVSRERQAVKETNLGAPSTTLGAAVLPTIAICDVDLVHQSHISDEEELRFQMGV
                     AVYGMERDQHNGGRAYRWGVQTVHHRRGVRLRMVNIGSTTAIDRFDRIGYPVCRVCGQ
                     SISPLSSERQREHFLQTHQERCGKPIIPVGFFADVVADILSLPACPDQTTAYSVLEAL
                     RFAAARVLDMHLDDLQILVIGHVDRDEVDGLLWDPMPGGSGLLDQICDRFEEIAAVAQ
                     DVVQTCPAVCEKSCIDCLQTFRNGYYHKYLDRKVAQERLAMWGRRLSVSHEIPVKQPS
                     PSHEEGSHPVNEAERRLRQLLLAAGFEEGIRGRQIRLDRAIGTTTPDVIYRAHHHDED
                     EGVCIYLDGLSGHLHGNAATAEQDQRIRSWLRNNGYEVIEIAASDLFDEGAMTRHFRR
                     LAGFLNAADIRDALRRDATWFQRLRKADAAVKEFVLHLVKPKPEERFTNCVPLIPLKV
                     AAGNFGEPQTVGDGEWDYDWVEINTHRHLRKGMFVAQVVGKSMEPMIPDGAYCLFCSQ
                     VEGTRQSRTVVVLLRDTTDPETGQRFTVKRYESEKAVSADGTWRHIKVLLKPVNKDFN
                     PIELTCEDEGSVEVVAEMLEVLG"
     gene            5339..5542
                     /locus_tag="PLR47_06600"
     CDS             5339..5542
                     /locus_tag="PLR47_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12651.1"
                     /translation="MLAIRKPLLSLDYGFYRNKEGDHFSDVIIFLALMGIIIYRSQPN
                     QPPCSELSTYEMSVMVSQQAAEN"
BASE COUNT         1249 a         1464 c         1650 g         1270 t
ORIGIN      
        1 acggtgtatc ctccggaaga gatctgctct cgcgaaatga tgcgtacggc cggccgtcag
       61 ccgcgcatcc tgcttactaa tgtgaagcag ttggagctgc tgctcacgcg tcagcgggac
      121 gtcgaactgt ttgccgatgc gagacttgat tatctggtct ttgatgaggc ccacaccttt
      181 gccggcgctc agggagctga aacagcctgc ctcatccgcc gtttgcgtgc attttgcagc
      241 cgggatgccc gcgacacggt ctgtattgcc acatcggcaa ccattgcgga tcagcggaac
      301 ccgaatgccg ccggtgattt tgccggccgt ttttttggcg tggacaaggc gtcggtcgtc
      361 acggtgagtg aggcgtatga acaggaggta tgggcgccca aacgcaaaac gcctcctctt
      421 cccaggaagg ataccggtga gatactgaat gcgtgcgtgt cggcggtaga agaagtcaat
      481 ggcgatggag caacgctgcg cgcagtctat cgcgatctgg cttcagctga tcttccgacg
      541 ggaaatggtc cggaagtctt gtatgacgtt ctttcacaaa atgaattggt ctttcaattg
      601 agcgagtcgc ttgccttgcc ccgtccgcta cacgaactcc ccgaaatgat aggtgagaga
      661 atcggccgat ccgtaaacga ggctgaaatt ttgtcctggc ttacgttggg tgcggcggcg
      721 agaaaggaag gaaggccgct cgttcgaccg gttgtgcacg gctttattcg ggggatcagc
      781 ggcgctgttg tgaccttccc ggaaagtgaa gcagggccga agctctggct cgctgccgaa
      841 gatgaaatcg aggcgaaagg cgagaaggaa caaaatgccc atttccccgt aaccacctgc
      901 accacctgcg ggcaacacta ttttattact tatctgaagg actttgattt ttccggtcgg
      961 cgtcccggcg gcggcgaggc tgaaggagat ggttcattct ggttaccctt ggaagaaagc
     1021 cagggcggca agcgtgtagt attgctggat cgcatcgtgg gcggcagtga tgatgaagat
     1081 ctcgatgata atgagcgaat ttctgcgctg tatttttgcc ggcgctgcgg cgccgcgcat
     1141 cccacgagtg tcagccgttg ctgccattgc ggaatgacag gggttccggt tgtgctttat
     1201 gctgtccggc agaaaaagga caatccgggt tttctttcga gttgcctgtc ctgcggagcc
     1261 aatggacggg tgatcgggac gacttaccgg gaaccggccc gtcctgtacg cgccaccaat
     1321 gttgcggacg ttcatgtcct ggcgcaggat atggtgcaca actcggagag accccgtttg
     1381 ctggttttct gcgacaaccg tcaggatgcg gcctttcagg ctggatggat gaaggatcac
     1441 gcccgacgct tccgtcttcg cgcactgatg gctgatggtc tcaaaggcgg ggccatgtcc
     1501 attggcgatc tggccatgca tctcgacgca atcctggaaa aggatgaatc cctctctcgc
     1561 gctcttgtcc ccgaagtctg gcaggttgtc cgccgcgaag gcagcggtgg ccgccacgaa
     1621 caggagcggc gaaaatttct ccgaattcag gtgcttcggg aagtcacact ttccgcccgt
     1681 caggccatag gccttgaacc ctgggggcgg atgaaggtgg actatgaagg actcgacccg
     1741 gcattgccgt ggatccagga aaatgcgcat gctctcggca tgccgcccga ggatctccgc
     1801 gatggcgttg ccggtcttct cgattattta cgccgcaagc gcgttctttt tgatccggaa
     1861 agggaggtct ttactcacta ctggatggat ggcgatctgg aaatccaaca gggatacctg
     1921 cctcaactgg gcgcccccgt cggcacaaaa ttccataggg aagcggatga aaaacaggaa
     1981 ttagttgcgc agtggttcag tgagcgcggc gatacgacga tccgtcagat ggccagaaag
     2041 tggggagtgc cttcggagaa tgcccaagga tttctggaag ggctgttccg gtttctcgtg
     2101 gataaaagat tgcttgtacc ggtccgcctc aaggggtcca ggggcaatcc tctgccgaat
     2161 gtcagcggcg tctatcagct taatgccgat gtcctgcgcc tccagcaaaa tcacggggtt
     2221 tatcgctgcc gcaactgccg ccgccggaca acccgccggt caccaaatat gctttgcccc
     2281 gcctggcgct gtgacggcag cctggaattt gttcgggaag atccggataa ttatgatctt
     2341 cagctcctgg atcagggata ttccatgctc cgtccggaag agcacaccgc tatggtgcct
     2401 cacgacgaac gggaacgcct tgaaaatctg ttcaagggtg attcggaagc agtcaacgcc
     2461 tttgtctgca cgccgactct cgaacttggc gtggatatcg gcaagctcga tgcaattctg
     2521 atgcgtaatg tcccgccatt gcccgccaat tactggcagc gggccggacg cgccggccgc
     2581 cgacaccgta tggcggttga catgacctat tgccggccgg tttcgcatga ccgcgcctat
     2641 tttgccgagc cgctcaaact tctggccggt caggttgatc cgcccgcttt caacctgcgc
     2701 aatgaactga tggttgccaa acatgtccac gcggcagtta taaccaggct gcatcaattt
     2761 atgcgtgaca gttcacggac accgacggaa cggcaggata ttgaagatac gctgaaaaag
     2821 tgtctgcccg atcaggtttc cgcatatctc ttcgacggcg gtcgtgtcag agataggctt
     2881 gcggacctta ctcctctgcg aagactaatc gaacagaatg tcgatgacct ctgtgaatat
     2941 gtggaacgcg tctttcgaca gggatggccg acggcggatg cggatgtgac aaagccccag
     3001 gccctccgcg atcacgtgca agggatgacc gattcgctcc tggacgtgct tgcacgtctg
     3061 gaacggcgtc tgctgtgggc gatggaacag attcgacgac tcaacggtgt ccgggaaagg
     3121 cagggtgatc tggaaccgga agacgaagcc ctgttcagac gctgcgatgc cctcgtgaag
     3181 cgtctgaaag gtacggcacg gcgcacccgc cgcgaagcgg aggggtatga cgacgttaat
     3241 acattcggcg tgctggcggc ggaaggattc ctccccggct atggcctgga ggtcggctcc
     3301 attttaggca cggccgaaat tccattctgg cgcaccggcg ccatggcgtt tgtcctgccc
     3361 cgaccccccg gttttgctct cagggaatat gtcccgggca acttgatcta cgccaacggg
     3421 aaccgttttg tcgcccgccg ttttcatcgt gacatagacg agcaaaaggc tgaaatgcct
     3481 gtttttgagg tttccagaga acgacaggct gtaaaagaaa cgaatctggg cgcgccgtca
     3541 acgaccctgg gggccgctgt cttgccgacc attgccattt gcgatgtcga tctggttcat
     3601 cagtcgcata tttcggatga agaggagctt cggttccaga tgggcgtggc tgtttacgga
     3661 atggagcgcg accagcacaa cggcggccgg gcttaccgct ggggtgttca gacggttcac
     3721 catcggcgcg gcgtaaggtt gcggatggtg aatatcggtt caaccacggc gatcgatcgt
     3781 ttcgacagaa tcggctatcc cgtgtgccgc gtctgcgggc aaagcatttc ccctctctcc
     3841 tctgaacggc aacgtgagca tttcttgcag acgcatcagg agcgatgcgg aaagcccatt
     3901 attccggtcg gcttttttgc cgatgttgtg gccgatatct tgtcgttgcc tgcatgtccg
     3961 gatcaaacaa cagcatacag tgtcctcgaa gccttgcggt ttgcagcagc gcgggtgctg
     4021 gacatgcacc tggacgattt gcagattctt gtcatcggtc atgtggatcg cgacgaggtt
     4081 gatggccttt tgtgggatcc gatgcccggc ggctcaggac ttcttgatca gatctgtgat
     4141 cgatttgagg agattgccgc tgtggcgcaa gacgtggttc aaacctgtcc tgcggtctgc
     4201 gaaaaatcat gcatcgattg cctgcaaacc ttccgcaatg gatattacca caaatacctt
     4261 gaccgtaaag tcgctcagga gcgtcttgcc atgtggggaa gacgattgtc tgtgagccat
     4321 gagattccgg taaaacagcc gtctccgtcg catgaagaag gaagccatcc ggttaatgaa
     4381 gcggaacgac gtttaagaca gcttcttttg gccgccggct tcgaagaagg cattcgcgga
     4441 cggcagatcc gtcttgaccg tgccatcgga acaaccacgc cggatgtaat ctaccgagcg
     4501 catcatcatg acgaggacga aggagtttgc atttatctgg acggtttgag cggtcattta
     4561 cacggaaacg ccgccaccgc agagcaggat caacgcatcc gctcctggct gcgcaacaat
     4621 ggctacgagg tgatcgaaat agccgcaagc gacctttttg acgaaggcgc catgacaaga
     4681 cacttccgca ggctggccgg ttttctgaat gcagcagata tccgggatgc tctacgcagg
     4741 gatgcgacgt ggtttcaaag actgagaaag gcggatgccg cggtaaagga atttgttctc
     4801 catctggtga aaccaaaacc cgaggaacgc tttacaaatt gcgttccgct gatacccctg
     4861 aaggtagcgg caggtaattt cggcgaaccc cagactgtcg gcgacggcga gtgggattat
     4921 gattgggtag agatcaacac gcacagacat ttgcgcaagg ggatgtttgt ggcgcaggta
     4981 gttggtaaat ccatggaacc tatgattccc gatggggcat actgcctctt ctgttcgcag
     5041 gtcgaaggga cacgccaaag ccggacagtt gtcgttcttt tgcgcgacac gacagacccg
     5101 gaaaccggac aacgcttcac ggtcaaacgc tacgaaagcg aaaaagcagt ctccgcagat
     5161 ggaacctggc ggcatattaa agtgttacta aaacccgtca ataaggattt caaccctatc
     5221 gaactgacct gcgaggatga aggcagcgta gaagttgttg ccgagatgct ggaagtgcta
     5281 ggataataat tctatgatct catgatgtta catgtctagg ggctaaaagg agttcagaat
     5341 gctggccatc aggaaaccat tgttatcact ggattacggt ttttacagga ataaagaagg
     5401 cgaccatttc tctgatgtca tcatttttct tgcattaatg ggaattatca tctacagaag
     5461 tcagccaaat caaccacctt gcagcgagct atcgacgtat gaaatgagcg tcatggtatc
     5521 acagcaagct gcggagaatt aacgcttgtt ccacaacttc tggattaaaa agtgcaattt
     5581 gactggtttt gcagcagaat gccgaaaata atccgcgttg acattttgac caa
//
LOCUS       DAOSIM010000129         5618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_12954_length_5618_cov_7.974834, whole genome shotgun sequence.
ACCESSION   DAOSIM010000129 DAOSIM010000000
VERSION     DAOSIM010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12954_length_5618_cov_7.974834"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /gene="fusA"
                     /locus_tag="PLR47_06605"
     CDS             <1..697
                     /gene="fusA"
                     /locus_tag="PLR47_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417795.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOG12652.1"
                     /translation="TVISGMGELHLEIIVDRLNREFNARVNVGRPRVVYRETIQKRAQ
                     AEHLFEKELGDKKHFGQVRLELSPRKRGIGNEIVNAIPEEMIPAEYHEAIEESIREAM
                     SSGVLNSYPVIDVAVRLTGGAFREGEASVQGYKIAAATAFRDACLAAEPVMLEPIMAV
                     DIITPSEFMGDVIGDINARRGEIQAVNPKGPVSEIKAKVPLKALFGYSTDLRSATQGR
                     AVFTIMFEEYNKA"
     gene            801..2180
                     /locus_tag="PLR47_06610"
     CDS             801..2180
                     /locus_tag="PLR47_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOG12653.1"
                     /translation="MAVLKSAWERLRDYPIFESEREAYPQTFSEELNDQCGRILFVVC
                     LITTVAWLPYIYLDAGLHPEEPLLPVIRMGLSVLSLTLFILHVSKRFPRHNMLFLVLI
                     GAYLAVGCGLITALADADPAYMGGYIFILTVMALVPVDRRYAIIIIAASLLTFFVTGR
                     FENISFDRTSDRYSVLDLSSATVVAIAFIYLLHNTRLASWLKSRKIEQQSEEMRSDKE
                     KIDNLLLNILPPAVAEELKGQGYVKPVFYPSATIVFTDFVGFTKISETLTPDQLVREL
                     DSAFSRFDRVMDKYGLEKLKTIGDSYMYAGGVPLPNATHAIDAVLGALEIQSFVEQIN
                     LEKSQSGRPVFEIRIGINSGPLMAGVVGEKKFVYDVWGDSVNLASRMESSGGKGRINI
                     SESTHARIRDYFETEARGQVLAKNKGAVSMYFVNRLKKEFASDPEGRTPSESFWSIYK
                     KLQEENTAA"
     gene            complement(2197..3522)
                     /gene="trmFO"
                     /locus_tag="PLR47_06615"
     CDS             complement(2197..3522)
                     /gene="trmFO"
                     /locus_tag="PLR47_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOG12654.1"
                     /translation="MKSGRVMIIGGGLAGCEAAWQLLRRGHGVDLYEMKPRRFSPAHK
                     TEHLAELVCSNSLKSNQPDSAPGLLKEEMRRLGSLILSAADQTAVAAGSALAVDRNEF
                     SRRVEERLLGQEGFSLHRAEVADIPQDGLTILATGPLTSDALAQSISRMLGGNSYLYF
                     YDAIAPIVEADSIDMDKAYWASRYGKGTPDYLNCPLSREEYQRFREALLTGEKVAARP
                     FEDVRHFEGCLPIEILAARGEETLAFGPMKPVGLINPNTGKMPCAVVQLRRENSAGTL
                     FNLVGFQTKLTWPAQERIFRMIPGLERAQFARMGSIHRNTYIHSPSLLSPSLQFRKNP
                     DVFFAGQISGVEGYMESTAMGLLAGLNAAFILEGRSLPPPPRETAVGALAHYITSPES
                     AENFQPMNIHFGILPPVEAPGMKKKERHLFCVRRSLEALSDWMRDQGLA"
     gene            complement(3519..>5618)
                     /gene="topA"
                     /locus_tag="PLR47_06620"
     CDS             complement(3519..>5618)
                     /gene="topA"
                     /locus_tag="PLR47_06620"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOG12655.1"
                     /translation="APDPDREGEAIAWHIADVIGAKNKKIYRVLFNDLTRNTVLEALQ
                     KPLKLDFDKYEAQQTRRILDRLVGYQISPILWDKVKRGLSAGRVQSVAVRIICEREEE
                     IGKFVPEEYWNLVAQFEASVPPSFEAKLIKIGGKKVKVSNAEEAGKLAADIQAAEFTV
                     EKLEKKEVKRSALPPFTTSKLQQEASRWLKFSAKKTMSVAQKLYEGIELGPEGSVGLI
                     TYMRTDSYRIAEEPLAAVRGYIKENYSDAYVPAKPHFYKNAQKAQDAHEAIRPSSMAH
                     KPQDIKPYLTPDQFRLYQLIWNRFVASQMNPAVFDQTTIDIAGAGCTFRAQGQVMKFP
                     GFTIVYTEGKDEKEEENGNGKDKLLPDVAENEKIRLAKLDTQQKFTQPPPRFSEASLV
                     RELEENGIGRPSTYASILSTIQDREYVRLEKGKFFPTELGKIVTELLVHSFPTILDMA
                     FTADMENKLDAIESGANQRVRTLQDFYSLFSGELSQAKKGMRNVKQEETPTDLVCEKC
                     DSPMVIKWGKNGRFLCCSNYPECKNTKNFTHDENGKVKHMETQTTDVKCNKCGKNMVV
                     KEGRFGQFLACSGYPECKNTMNATKNENGDVVAQEAPQTDEVCDLCGKPMAVKRGRYG
                     QFLGCTGYPECKNIKKLGRDGKVTQKEEVVLSDEVCDLCGKPMAVKRGRYGQFLGCTG
                     YPECKNIKKIPRKKSEE"
BASE COUNT         1119 a         1630 c         1604 g         1265 t
ORIGIN      
        1 aaccgtcatt tccggcatgg gcgaactgca cctggaaatc atcgtggatc gcttaaaccg
       61 cgaattcaac gcccgcgtca atgtcggacg tccccgcgtc gtgtaccgcg aaaccattca
      121 aaagcgcgcc caggccgagc atttgtttga aaaggaactg ggggacaaaa agcatttcgg
      181 ccaggtccgc ctcgaactgt cgccgcgaaa gcgcggcatc gggaatgaaa tcgtgaatgc
      241 gattcccgaa gaaatgatcc ccgccgagta tcacgaggcg atagaagaga gcatccgcga
      301 ggcgatgtcc agcggcgtgc tcaacagcta tccggtcatc gatgtcgccg tccggctgac
      361 cggcggcgcg ttccgcgagg gagaagcttc cgtccaggga tacaagattg ccgccgcgac
      421 ggcatttcgc gacgcctgcc ttgccgcgga gcccgtcatg cttgaaccga tcatggcagt
      481 ggatatcatc acccccagtg aattcatggg cgatgtcatc ggcgacatca atgcccgccg
      541 cggagaaatc caggcggtca atcccaaggg gcccgtctcc gaaatcaagg ccaaggtgcc
      601 gctcaaggcc ctgttcggtt attccacgga tctgcgctcc gccacacagg gccgggccgt
      661 gttcaccatc atgtttgaag aatacaacaa agcatgagac gatgagggaa gacattcgcc
      721 gatgaaatga aaaacgtttg taattctgcg aacagaacgc ccggccggaa gggccgggga
      781 gaattctgaa ggaggcaaac gtggcagttc tgaagagcgc gtgggagcga ttgcgcgatt
      841 accccatttt cgaaagtgaa cgagaggcct acccgcagac tttttccgaa gaattaaacg
      901 accaatgcgg ccggatatta tttgtcgtgt gcctcatcac gaccgtggcc tggctgccct
      961 acatttacct cgacgccggc ctgcatccgg aagagcccct gcttcccgtg atccgcatgg
     1021 ggctgtccgt cttgagcctc acgctcttca tcctgcacgt atcgaagcgc ttcccccggc
     1081 acaacatgct gtttctggtt ctcatcggcg cttatctggc cgtcggctgc gggctcatca
     1141 ccgctctggc cgatgccgat cccgcgtaca tgggaggcta cattttcatt ctgaccgtga
     1201 tggccctggt gccggtggac cggcgctatg ccataatcat catcgcggct tccctgctga
     1261 cgttttttgt caccggccgc tttgaaaata tttccttcga ccggacgagc gaccgctaca
     1321 gcgtcctgga tttgtccagc gccacggttg tcgccattgc gttcatctac ctgctgcaca
     1381 acacaaggct ggccagttgg ctgaaatcca gaaagattga acagcagagc gaggaaatgc
     1441 gatccgacaa ggaaaaaatc gacaacctgc ttttgaatat tctgccgccc gccgtggccg
     1501 aggagctgaa aggccaggga tacgtcaagc ccgtgtttta tccgtccgcg accattgtct
     1561 ttacggattt tgtcggcttt accaagattt ccgaaacgct gacgcccgac cagctggtcc
     1621 gtgagctgga ttcggccttt tcccggtttg accgcgtgat ggacaaatac ggcctggaaa
     1681 aactcaagac catcggcgat tcctacatgt atgccggcgg cgtgcctctc cccaacgcga
     1741 cgcatgccat cgacgcggtt ctgggcgctt tggaaattca gtcgtttgtc gagcagatca
     1801 atctggaaaa gtcgcagagc ggacggccgg tctttgagat ccggatcggc atcaacagcg
     1861 gcccgctcat ggcgggcgtc gtgggggaaa agaagtttgt ctatgacgtc tggggcgatt
     1921 ccgtcaacct ggccagccgc atggaatctt caggcgggaa gggaagaatc aacatttccg
     1981 aatccaccca tgcaaggatc agggactatt ttgaaaccga ggcccgcggg caggtgctgg
     2041 cgaaaaacaa aggggcggta tccatgtatt tcgtcaaccg gctcaaaaaa gaatttgcct
     2101 cggatcccga aggccgaacg cccagcgaaa gcttctggtc tatttacaaa aagcttcagg
     2161 aagaaaatac ggcggcataa cgcctgtcat gatttgtcac gccaggccct gatcccgcat
     2221 ccaatccgaa agcgcctcaa gggagcggcg cacgcagaaa agatgccttt cctttttctt
     2281 cattcccggg gcttccaccg gcggaagaat tccaaaatgg atgttcatgg gctggaagtt
     2341 ttccgccgat tcgggagacg tgatgtagtg cgccagcgcg ccgacggccg tctcccgcgg
     2401 aggcggcggc agggatcttc cctccaggat aaacgcggcg ttcaatcccg ccagaagccc
     2461 catcgcggtg gattccatgt atccttccac tccgctgatc tgtccggcaa agaaaacatc
     2521 gggatttttt ctgaattgaa gggaaggcga aagaagggaa ggggagtgaa tgtaggtatt
     2581 gcggtgaatg ctgcccatcc ttgcgaattg cgcgcgctcc aggccgggga tcatccggaa
     2641 aattcgctcc tgcgcgggcc aggtcagctt ggtttgaaaa ccgaccaggt tgaaaagcgt
     2701 gccggcgctg ttttcgcgcc ttagctggac cacggcgcag ggcatctttc cggtattcgg
     2761 attgatgagc cccaccggct tcatggggcc gaacgccagc gtttcctctc cgcgcgccgc
     2821 cagaatttcg atcggcaggc atccctcgaa gtggcggacg tcttcaaacg gacgcgcggc
     2881 gactttttct cccgtcagca gcgcctcgcg gaaccgctgg tactcctccc gggaaagcgg
     2941 gcaattcaga taatccggcg taccctttcc gtagcgggag gcccagtagg ctttatccat
     3001 gtcgatggaa tcggcttcca cgatcggggc gatggcgtca taaaaataca gataggaatt
     3061 tccgccgagc attcgggaaa tggactgtgc aagggcgtcg gatgtgagag gtcccgtggc
     3121 gaggattgtc aggccgtctt gaggaatgtc cgcaacctcg gcgcgatgca gggaaaaacc
     3181 ttcctgcccg agcagccgct cctcgacccg gcgggaaaat tcattccggt ccacggccag
     3241 cgcgctgccg gcagccaccg ccgtctgatc ggcggcggac aggatcagcg aaccgagcct
     3301 gcgcatctct tccttgagca ggccgggcgc actgtcgggc tgattggatt tgagagagtt
     3361 gctgcagaca agctcggcca gatgctcggt tttgtgggcc ggggaaaaac gccgcggctt
     3421 catttcgtaa agatcgactc cgtgcccgcg ccgcaggagc tgccaggccg cttcacagcc
     3481 cgccagcccc cctccgatga tcatgacccg cccggatttc attcttccga ttttttcctg
     3541 gggatctttt taatgttctt gcattcggga tagccggtgc agcccagaaa ctggccgtag
     3601 cgcccgcgct tgaccgccat gggctttccg cacagatcgc agacctcgtc ggaaagaacc
     3661 acttcctcct tttgggtgac tttgccgtcc ctgcccagct ttttgatgtt cttgcattcg
     3721 ggatagccgg tgcagcccag aaactggccg tagcggccgc gcttgaccgc catgggcttt
     3781 ccgcacagat cgcagacctc gtccgtctgc ggagcttcct gggcgaccac gtcgccgttt
     3841 tcgtttttgg tcgcgttcat cgtgttcttg cactccggat agccggagca ggccagaaac
     3901 tgcccgaaac gtccttcctt gacgaccatg tttttaccgc acttattgca tttaacgtcg
     3961 gtggtctggg tttccatatg tttgaccttt ccgttttcat cgtgggtgaa gttcttggtg
     4021 tttttgcatt ccggataatt ggaacagcac aggaaccgcc cgttttttcc ccatttgatc
     4081 accatgggag aatcgcattt ttcacaaacc aggtcggtcg gcgtttcttc ctgcttgacg
     4141 ttgcgcatgc cctttttcgc ctggctcagc tccccggaaa aaagactgta gaaatcctgc
     4201 agcgtccgga cgcgctgatt ggcgccgctt tcaatggcgt caagcttgtt ttccatgtcc
     4261 gcggtgaaag ccatatccag aatagttgga aaactgtgca ccagcagctc cgtgacaatt
     4321 tttcccagct ccgtcgggaa gaattttccc ttctccagac gaacatattc ccggtcctga
     4381 atggtggaaa ggatcgatgc atacgtgctg gggcgtccga tgccgttttc ctccagttcg
     4441 cgcaccagcg acgcctccga aaaacgcggc gggggctgag tgaacttctg ctgcgtgtcg
     4501 agcttggcga gcctgatttt ttcattttcc gcgacgtcgg gcagcagctt gtcctttccg
     4561 tttccgtttt cctcttcctt ttcatccttg ccttccgtgt acacgatggt aaagccggga
     4621 aacttcatga cctggccctg cgcgcggaag gtgcagcccg cgccggcgat gtcgatcgtc
     4681 gtctgatcga aaacggcagg gttcatctgg cttgccacaa agcggttcca gatcaactga
     4741 taaagccgga actggtcggg cgtcaggtag ggcttgatgt cctgcggctt gtgggccatc
     4801 gaagaaggcc ggatggcttc atgggcgtcc tgcgccttct gtgcgttttt atagaaatgg
     4861 ggttttgcgg gaacatatgc gtcggaataa ttttccttga tgtagccgcg gacggccgcg
     4921 agcggctcct cggcaatccg gtaggaatcc gtccgcatgt aggtgatcag acctacggac
     4981 ccttccggcc ccagttcgat gccctcgtaa agcttctggg cgacgctcat cgttttcttg
     5041 gcggaaaact tgagccagcg cgaggcttcc tgctgaagct tgctggtcgt aaagggcggc
     5101 agggccgacc gcttcacttc ctttttctcc agcttttcga cggtgaactc cgccgcctga
     5161 atgtccgccg ccagtttgcc cgcttcttcg gcgttggaca ctttcacctt tttcccgccg
     5221 attttgatca gcttcgcttc aaaagagggc ggaacagagg cttcaaactg cgccacgaga
     5281 ttccagtact cttccggcac gaacttgccg atttcctctt cccgctcgca gatgatgcgc
     5341 acggccaccg actgcacccg tccggcggag aggccccgtt tgactttgtc ccagaggatg
     5401 gggctgatct ggtagccgac gaggcggtcg aggatgcggc gcgtctgctg ggcctcgtat
     5461 ttgtcgaaat ccagcttcag aggcttctgg agagcctcca gcacggtgtt cctggtcaaa
     5521 tcgttgaaga ggacccggta gattttcttg ttctttgcgc cgatgacgtc ggcgatatgc
     5581 caggcgatgg cttctccttc gcggtccggg tcgggcgc
//
LOCUS       DAOSIM010000130         5604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_12991_length_5604_cov_6.773833, whole genome shotgun sequence.
ACCESSION   DAOSIM010000130 DAOSIM010000000
VERSION     DAOSIM010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12991_length_5604_cov_6.773833"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PLR47_06625"
     CDS             complement(<1..972)
                     /locus_tag="PLR47_06625"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOG12656.1"
                     /translation="MKDSMIHPVRIQDNTFRDGHQSIHATRMKTEDMIPIAEEMDDCG
                     FWAMEVWGGATFDAMHRFLEEDPWRRPGILKKYIRKTPMSMLLRGQNLVGYRHYADDV
                     VRAFVDRACEIGIDIFRCFDALNDFRNLETCVERIKANGKHVEGTVCYSLTEGHLGGE
                     IYNLDFYVKKAGELEQMGADTLCIKDMAGLLSPYDAFNLFTELKKAVRVPLHLHTHYT
                     SGMGSMTCLKAVEAGVDIIDTCLSPYALRTSMPACEPIVAALQGSERDSGINLNKLLE
                     MSEHLEKIAPKYQHLLADQRLSIIDNGVLAHQIPGGMISNLTSQLKEM"
     gene            1362..3494
                     /gene="scpA"
                     /locus_tag="PLR47_06630"
     CDS             1362..3494
                     /gene="scpA"
                     /locus_tag="PLR47_06630"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOG12657.1"
                     /translation="MTAHQNMKEWKEAALRELKGKPLESLNWATPEGIVVKPIYTADD
                     LEGLDTVNTLSGLAPYLRGPSATMYANQPWTIRQYAGFATAKESNEFYRKNLAAGQKG
                     LSVAFDLATHRGYDSDHPRVAGDVGKAGVAIDSVEDMKILFDQIPLDKVSVSMTMNGA
                     VLPILAGYIVAAEEQGVAQKQLAGTIQNDILKEFLTRNTYIYPPRPSMRIVSDIIAYS
                     SKNMPRYNTVSISGYHIMEAGADSVLQTAFTLADGKEYIRAAIDGGLKIDDFAPRLSF
                     FFGVGMNFFMEIAMLRAARYLWHQIVGEFHPTNPRSQVLRTHVQTSGWSLTQQDPYNN
                     IIRTTLEALAAALGGTQSLHTNSFDEAVSLPTPLSSRIARNTQIVIQEESQICRVVDP
                     LGGSYYVEALTASIIREAKKIMDEIEELGGMARAIETGMPKMRIEESAARRQARIDQG
                     KDTIVGVNKYKIKEDAPIDVLDIPATVRDEQIARIKAIKAKRDNAAVVKALEKITHVA
                     EKGGNLLEASIEAVRLRATVGEVSDAMEKVFGRYVATTRVISGAYASEYGDDDVIRSL
                     RARTEKFLEKNGRRPRQLVTKMGQDGHDRGIKVVATAYADIGFDVDISPMFQTPEEAA
                     KMAIENDVHVVGVSSLAAGHKTLVPELIEQLKKMGGQDIIVVVGGVIPPADYGFLYEK
                     GVKGIFGPGTAVTDSADRVLRLLEEKYG"
     gene            complement(3677..5512)
                     /locus_tag="PLR47_06635"
     CDS             complement(3677..5512)
                     /locus_tag="PLR47_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOG12658.1"
                     /translation="MEFKYDVRDLKFILKEWLPTGEVLACDRYKDNFSMDDIDMLLNE
                     GCKVAREVVNPINAPGDKIGVKFDKGVVTPVPGFKEAYQFLQQNGWGSSSECIKVETG
                     MPLVLYKACAEMNTAACPALTSCIKLTSGAANLILRFGTKEDQDRFVPKMLDGSWQGT
                     MCLTEPSFGSDTGDIITRSYPTDDPRIWKIKGTKMFITAGDQGTCENTIHLVLARPEG
                     AAAGSPGIGLYVVPKYWVNEDATMGDFNDVATVGVEHKMGLHAQATALLNFGDDDNCR
                     GIMLGAAPDEKGFSKGLAMMFHMMNESRIGTGHNANTQGAAAYYFASQYAAERVQGRP
                     FGIKNAERVPIIQHEDIRRMLLDMKAHVEGIRAMIFRGFYYLDIESNSPDKEKAKKCS
                     SFAEILTPIVKCYGSEASLGVIAQAIQVLGGVGYTLDYPVEQYLRDSKILTIWEGTSF
                     IHANDLIGRKMRMKDGAPFAAWMQDIGNFIEANQNAEGFEKEMKSLSDAFAAAEEVRT
                     IYNAWWADMEHKRRLIPLYAQKALFVFAYVYVAMCLLDQALIAAKKSQDLKPGEGDHT
                     FYAGKIASARYYANNILPNVPMLAALIKSEEDTVLACPEEALVVS"
BASE COUNT         1146 a         1621 c         1618 g         1219 t
ORIGIN      
        1 catttccttg agctggctgg tcaggttgga aatcatgccg ccgggaatct gatgggccag
       61 cacgccgttg tcgatgatgg aaagtctctg atccgcgagc agatgctggt atttgggggc
      121 gatcttttcc agatgttcgc tcatttccag gagcttgttg aggttgattc ccgagtccct
      181 ctccgatccc tgaagcgccg ccacaatggg ttcgcaggcg ggcatggacg ttctcagggc
      241 gtagggagac aggcaggtgt cgatgatgtc cacgccggct tcaaccgctt tcaggcaggt
      301 catgctgccc atgccgctgg tatagtgcgt gtgcagatgc agggggaccc ggaccgcttt
      361 tttcaactcg gtgaacaaat tgaatgcgtc gtagggagaa agcagaccgg ccatgtcctt
      421 gatgcagagg gtgtccgcgc ccatttgttc gagctctccg gcttttttta cgtagaagtc
      481 aagattgtaa atctccccgc ccagatggcc ttcggtgagg gagtagcaga cggttccttc
      541 cacatgcttg ccgttggcct tgattctttc cacgcaggtc tcgaggttgc gaaagtcgtt
      601 gagcgcgtcg aagcagcgga agatgtcaat gccgatttcg caggcccgat cgacaaaggc
      661 ccgcaccacg tcgtctgcgt agtggcggta ccccaccagg ttctgtccgc gcagcagcat
      721 gctcatgggc gttttccgaa tgtacttttt cagaattccg ggccgcctcc acgggtcttc
      781 ctccagaaaa cggtgcatgg cgtcaaacgt cgcgccgccc cacacctcca tggcccagaa
      841 cccgcagtcg tccatctcct cggcgatcgg gatcatatcc tctgttttca tccgggtcgc
      901 gtgtatggac tgatggccgt cccggaatgt gttgtcctga attctgacgg ggtggatcat
      961 tgaatctttc atgcgaaact ccttgccgcg tacgaatatg ccgcgccgta gattacagca
     1021 aatagcatgc cagccgtttc ccgctttcaa tgaagggcgg cgcggtaaat gtccggcgtt
     1081 cttcggttct caatcccgct ttggcggaac gagcgttgat tctccgcagc ttgccgcgaa
     1141 gcgtttgatt agatattatg ctattgccgc gcaaatgtgg tatgccacct ttcggtttgc
     1201 aggggagggg acgctgtata tcacctatac ggcttgtttc tgaactgtat attttataaa
     1261 caggctgccg ttttcgagga tggcccgcgc gcggccgatg caaaaatccg tcggggagcg
     1321 gttgcggccg cttcccgcat tatatcgaag gaggtttcaa cgtgaccgca caccagaaca
     1381 tgaaagaatg gaaagaagcc gccctccggg agctgaaggg aaagcccctg gagtctctga
     1441 actgggcgac gccggagggc atcgtcgtca agccgattta cacggcggat gatctggaag
     1501 ggctggacac cgtcaatacg ctgtccggtc ttgccccgta cctgaggggg ccttcggcca
     1561 ccatgtacgc caaccagccc tggacgatcc gccagtacgc gggtttcgcc acggccaagg
     1621 aatccaatga attctaccgg aagaatctcg ccgcgggaca aaagggcctg tctgtggcct
     1681 tcgacctggc cactcaccgg ggatacgact ccgatcatcc ccgcgtggcg ggcgacgtcg
     1741 gcaaggccgg cgtggccatc gactccgtcg aggacatgaa aattctcttc gaccaaattc
     1801 cgctcgacaa ggtttccgtt tccatgacga tgaacggcgc cgtcctgccg attctggcgg
     1861 gatacatcgt ggccgccgaa gagcagggcg tggcgcagaa gcagctcgcc ggcaccattc
     1921 agaacgacat tctcaaggaa tttctgacgc gcaataccta catctatccg ccccggccct
     1981 ccatgcggat cgtttccgac atcatcgcct actcgtcgaa aaacatgccg cgctacaaca
     2041 ccgtgtcgat cagcggctat cacatcatgg aagcgggggc ggactcggtc ctgcagacgg
     2101 ctttcacgct ggccgacggc aaggaataca tccgcgccgc catcgacggg ggcctgaaaa
     2161 tcgatgattt cgctccgcgc ctgtcgttct tcttcggcgt gggcatgaac tttttcatgg
     2221 aaatcgccat gctgcgggcg gcgaggtatc tgtggcacca gatcgtgggc gagttccatc
     2281 cgaccaaccc gcgctcgcag gttctgcgca cgcatgtgca gacgtccggc tggagcctca
     2341 cccagcagga tccgtacaac aacatcatcc gcaccacgct ggaggcgctg gccgcggcgc
     2401 tgggcggcac ccagtcgctg cacaccaact cgttcgacga ggcggtgagc ctgcccacgc
     2461 ctctgtcttc acgcattgcc cgcaacacgc agatcgtcat tcaggaggaa tcgcagatct
     2521 gccgcgtggt cgatcccctg ggcggctcct actacgtgga ggcgctcacg gcctccatca
     2581 ttcgggaagc caaaaagatc atggacgaaa tcgaagagct gggcggcatg gcccgggcca
     2641 ttgaaaccgg catgcccaag atgcgcattg aggagtcggc ggcccggcgg caggcccgca
     2701 tcgaccaggg aaaagacacc atcgtcggcg taaacaaata caaaatcaag gaagatgccc
     2761 cgatagacgt tctggacatt cccgccacgg tacgcgatga acagattgcc agaatcaaag
     2821 cgatcaaagc gaagcgcgac aatgccgccg tggtcaaggc cctggagaaa attacgcacg
     2881 tcgccgaaaa gggcgggaac cttctggaag cctcaattga agcggtgcgg ctgcgggcca
     2941 cggtgggaga ggtttccgac gccatggaaa aagtcttcgg ccggtatgtc gccaccacgc
     3001 gggtgatctc cggcgcttat gcctccgaat acggcgacga cgatgtgatc cggtcgcttc
     3061 gggcccggac ggagaaattc ctggaaaaga acggacgccg cccccgccag cttgtgacca
     3121 aaatgggaca ggacggccac gaccggggga tcaaggtggt ggccacggcc tacgcggata
     3181 tcggctttga cgtggacatc agtccgatgt ttcagacgcc ggaggaagcg gccaaaatgg
     3241 ccattgaaaa cgacgtgcat gtcgtgggcg tctcgagcct cgcggcgggc cacaagacgc
     3301 tggttccgga gctcatcgaa cagttgaaga agatgggcgg tcaggacatc atcgtcgtgg
     3361 tgggcggcgt cattccgccc gcggattacg gcttcctcta tgagaaaggc gtcaaaggca
     3421 tcttcggccc cggcacggcc gtcacggatt cggcggaccg ggttctgcgg cttctggaag
     3481 aaaaatacgg ataaaaacaa acgggcggcc gtgaagacgg ccgcccggaa aaaccggcaa
     3541 atttctcctc tctaagtctt tctgcgcctt cccagcttgc ccttcgatat cgagtcgcag
     3601 ggaacggtcg cgaccgttcc ctgcgactct ggatttttgg actgcatcac actttgctga
     3661 ttcattgcag ccggtcctag ctcacgacca gcgcctcctc gggacaggcg agcaccgtat
     3721 cttcttcact tttaatcagc gccgccagca ttgggacatt cggcagaatg ttgttggcgt
     3781 aatacctggc cgaagcgatc ttgcccgcgt agaaagtatg gtctccctcg ccgggtttca
     3841 aatcctgtga cttctttgcc gcgatgaggg cctgatcgag gaggcacatc gccacataga
     3901 cgtacgcaaa cacaaacagc gccttctggg cgtaaagcgg aatcaaccgg cgcttgtgct
     3961 ccatgtcggc ccaccaggcg ttgtaaatcg tccggacttc ttcggcggcg gcaaaggcgt
     4021 cggagaggct tttcatttct ttttcaaacc cttcagcatt ttggttggcc tcgatgaaat
     4081 ttccgatatc ctgcatccat gcggcaaagg gcgctccgtc cttcatgcgc attttccggc
     4141 cgatgagatc gttggcatgg atgaacgacg ttccctccca gatcgtgagg attttggaat
     4201 cgcgcagata ttgctccacc ggatagtcca gcgtgtagcc cacgccgccc agcacctgaa
     4261 ttgcctgcgc gatgacgccc agcgaagcct cggagccgta gcatttgacg atgggtgtga
     4321 ggatttcagc aaaagaagag cattttttgg ccttttcttt atcgggggag ttgctttcaa
     4381 tgtccagata gtaaaatccc cggaagatca tggcgcggat gccttccaca tgcgccttca
     4441 tgtccagcag cattcggcga atgtcttcgt gctggatgat gggaacccgt tcggcgtttt
     4501 taatcccaaa gggtcttccc tgaacgcgct cggcggcata ctgtgacgca aagtagtaag
     4561 cggccgcgcc ctgcgtgttg gcgttgtgtc ccgtgccgat gcgcgattcg ttcatcatgt
     4621 ggaacatcat cgcgagtccc ttggaaaagc ccttttcgtc gggcgcggcg cccagcataa
     4681 tgccgcggca gttgtcgtcg tcgccgaagt tcaaaagcgc cgtggcctgg gcgtgcagcc
     4741 ccattttgtg ctcgacgccg acggtggcga cgtcattgaa atcacccatg gttgcgtctt
     4801 catttaccca gtatttggga acaacataaa gtccgatgcc gggagaaccg gcagcagcgc
     4861 cttcgggacg cgccagcacc agatgaatcg tattttcgca ggtgccctga tcgccggcgg
     4921 tgatgaacat cttggtgccc ttgatcttcc agatgcgcgg gtcgtcggtc ggataggagc
     4981 gggtgatgat gtcgcccgtg tccgagccga agctgggttc ggtcaggcac atcgtgccct
     5041 gccaggagcc gtcaagcatt ttgggaacga aacggtcctg atcctccttt gtgccgaagc
     5101 gcaaaatcag attggccgcg ccggaagtca gtttgatgca ggaggtcagc gcggggcagg
     5161 cggcggtgtt catttcggcg catgctttgt aaaggacaag cggcatgccg gtttccactt
     5221 tgatgcactc gctcgacgag ccccagccgt tttgctgcag gaactggtag gcttccttaa
     5281 acccgggaac cggcgtcaca acgcccttat caaatttcac gccgatttta tcacccggcg
     5341 cgttgatggg gttgacgact tcccgggcga ccttgcagcc ttcgttgaga agcatgtcga
     5401 tgtcgtccat cgagaaattg tctttatacc gatcgcaggc caggacctcc cccgtcggca
     5461 gccactcctt taaaataaat ttcaaatccc gaacgtcata tttaaattcc atgtttgcgt
     5521 ctcctcaaat ttatttcctt cagcctatgg cccatcgcct atagcccatt gcctgttttt
     5581 tactttttcc catacccgat attt
//
LOCUS       DAOSIM010000131         5544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_13153_length_5544_cov_7.361815, whole genome shotgun sequence.
ACCESSION   DAOSIM010000131 DAOSIM010000000
VERSION     DAOSIM010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13153_length_5544_cov_7.361815"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLR47_06640"
     CDS             <1..685
                     /locus_tag="PLR47_06640"
                     /inference="COORDINATES: protein motif:HMM:NF022783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HOG12659.1"
                     /translation="HPLRLQRYSLADINPLTRPLQNVANVVRRNRKAAGPDNPFTELE
                     KAVSDYVEGVLDIYRDYRDLYQEEIFQQIYGNGWLRSLFPPVQDEPKPLAAKQDHPDY
                     DKRLAAMKQGGFAEGLIRIYMAAGGTNQGIKRKHFEVASEIAATHRVLSKLSPSQFKK
                     IMREQAAILQADEERALQALAALIKNRDDRMEALSIARRLFLADGVYNDEEKVLLEKI
                     RKGLGLEAV"
     gene            complement(858..1853)
                     /locus_tag="PLR47_06645"
     CDS             complement(858..1853)
                     /locus_tag="PLR47_06645"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HOG12660.1"
                     /translation="MPNANAAGKSARKKENTMTIVEVSPRDGFPEICNGLSTEDKIHY
                     INKLSETGLKKIECASFTHPRLLPEGYDAEKLVSGIAKKPEVTYVGLVPNEIGCRRAL
                     VTKIDELLVLLSASDTFNRINIGKSLRESLNKVLPTLLDAAARHGRSVRIYLMTAFGC
                     PYTGRVRPEEVAELLLKLSYMGASEVVLVDSTGMADPKSAKELIRALLELKLDIQLAV
                     HFHNTRGTALANCVAAYDEGIRIFDASLGGMSNTPYGVSELDIGYWNVPTEDIVHLFE
                     AMGIQTGIDLEKLLDCVRLAEKMAGKPLPGHILRARPATRITEIPRHLKEPRLLD"
     gene            complement(1922..2896)
                     /locus_tag="PLR47_06650"
     CDS             complement(1922..2896)
                     /locus_tag="PLR47_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOG12661.1"
                     /translation="MAGSAFDELIGDDEAFRRALVSAGRAAQSDISVLIIGESGTGKE
                     ILARAIHQTSRRKNKQLVDVNCAAIPDSLIESELFGYEKGAFTGARTEGRKGYFDEAH
                     EGTILLDEIGDSSLSVQAKLLRVLESGTFKRVGGIRNVRVDVRVISSTNQDLADLIGQ
                     KKFREDLFFRLNTFTIPLPPLRERRGDIDLLVEHFLKASGAAQKQKYRFSPEALAMLH
                     AYRWPGNVRELKGVVSYALNMTQSPVIDAGSLPNFLVSESRSRDGAAPPAAESGAASL
                     NLTQAVRDLERRLITEALAASSNRSEAIRALGISRRTFYLKLKEYGLE"
     gene            3165..5249
                     /locus_tag="PLR47_06655"
     CDS             3165..5249
                     /locus_tag="PLR47_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOG12662.1"
                     /translation="MAEIIGFSKEGPVGIITVSNPPVNALSQAVRSGIKAGIEKGIAD
                     AAVSAMVIICEGRTFIAGADIREFGRPPMEPHLPDVCQFIEDSPKPVIAAIHGTALGG
                     GLEIALSCHFRIALASAKVGLPEVKLGLLPGAGGTQRLPRVAGVQAALEMITSGTPVA
                     AARAKAMGILDEVVEGDLKAAALAFAKKVVLEKSPIRKVSQLQAKADSPSVFEDFAKA
                     NARKFRGFMAPFKCIEAVKAATELPFPEGMKREREIFVELHDSDQSKAQRHVFFAERE
                     VVRIPGLPDDQPTREIKSVGVLGAGTMGGGIAMCFANAGIPVMLLDVKQEFIDRGMAV
                     IKKNYANTVAKGSLKQEVMEKRLALIQPTLAYEDLKDVDLVIEAVFEDMAVKKEVFTK
                     LDAVCKPGAILASNTSYLNVDEIAAITGRPQDVMGMHFFSPANVMRLLENVRGAKTSP
                     EVYATAMKTGKKIGKVPVLVGVCDGFVGNRMLAKRTRECGFMLEEGALPWQIDRAIYN
                     FGFPMGPYRMGDMAGLDIGWRNRKAKFGSLTPREQKNNLLDKICEMGRYGQKTGSGFY
                     KYDEKRNATPDPMIEELIINHSKEVGIARRTLTDQEIIERAIYSMINEGAKILEEGIA
                     ARPLDIDVVWLYGYGFPVYLGGPMFYADTVGLKKVYDAVLKYRDSVGAEYWTPAPLLE
                     KLANEGKGFYSK"
     gene            5277..>5544
                     /locus_tag="PLR47_06660"
     CDS             5277..>5544
                     /locus_tag="PLR47_06660"
                     /inference="COORDINATES: protein motif:HMM:NF027503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HOG12663.1"
                     /translation="MMKYQEVYAHSISEPDAFWGKAGEAIEWTRRWDKVLDDSGKPFY
                     RWFAGGELNTCYNALDCHVENGRKDQTAIIYDSPVTDTVRKITYG"
BASE COUNT         1205 a         1560 c         1620 g         1159 t
ORIGIN      
        1 gcaccccctg cgcctgcagc ggtattcgct ggccgacatc aatcccctga cgcggccgct
       61 gcaaaacgtc gccaacgtcg tgcgcaggaa ccggaaggcg gcggggccgg acaatccgtt
      121 tacggagctg gagaaggcgg tttccgatta tgtggaaggc gtgctcgaca tttaccgcga
      181 ttaccgggac ctctaccagg aagaaatttt tcagcagatt tacggcaacg gctggctgcg
      241 cagcctgttt ccgcccgtcc aggacgagcc gaagccttta gccgccaaac aggaccatcc
      301 cgactatgac aagcggctgg ccgccatgaa gcagggaggt ttcgccgaag gattgatccg
      361 catttacatg gccgcgggcg gcaccaacca gggcatcaaa aggaagcatt tcgaagtcgc
      421 cagtgaaatc gccgccacgc accgggtatt gagcaaactg tccccgtcgc agttcaaaaa
      481 aataatgcgc gaacaggccg ccattcttca ggcggacgag gaaagggccc ttcaggctct
      541 tgcagcgctg atcaaaaaca gggacgaccg catggaggcg ctgagcatcg cccgccggct
      601 gtttctggcg gacggcgttt acaacgacga ggaaaaagtg ctgctcgaaa agatcaggaa
      661 ggggctgggc ctcgaagctg tatagatgat atacaaagca gaaaagggga cggttctatt
      721 tattttgcta ttagcagaaa aggggacggt tctatttatt ttgctattta ttttgagaga
      781 tgagcaacga aagatagttt ttccttcacg cttcagtgaa aaaatagaac cgtccccttt
      841 tttgtctttt ttgtccgtca gtccagcagc cgcggctcct tcaaatgccg tggaatctcg
      901 gtgatgcggg tcgcggggcg cgccctcaga atgtggccgg gcaaaggctt tcccgccatc
      961 ttttccgcca gccggacgca gtcgaggagc ttttccagat cgatgcccgt ttggattccc
     1021 atggcttcaa acagatgaac aatatcttcc gtcggaacgt tccagtatcc gatatccagc
     1081 tcgctcaccc cgtaaggcgt gttgctcatt ccccccagcg acgcgtcgaa aatgcggatg
     1141 ccctcatcgt aggcggctac gcaattggcg agggccgtcc cgcgcgtatt gtgaaaatgc
     1201 accgcgagct gaatgtccag tttcagctcc aggagcgccc ggatcagttc ttttgccgat
     1261 ttgggatcgg ccatgccggt cgaatccacc agaacaactt cggaggcgcc catatagctg
     1321 agtttgagca gaagctcggc gacctcctcg gggcggacgc ggcccgtgta ggggcatccg
     1381 aaggccgtca tcaggtaaat ccgaacgctc cggccatgcc gggccgcggc gtcaagaagc
     1441 gtcggcagca ccttgttgag gctctccctc aaggattttc cgatgttgat gcggttgaag
     1501 gtgtcgctgg cggaaagcag aaccagcagc tcgtcgatct tcgtcaccag ggcccgccgg
     1561 cagccgattt cgttgggcac cagccccacg taggtcacct ccggcttctt ggcgatgccg
     1621 gaaacaagtt tttcggcgtc gtacccttca ggaagcaggc gcggatgcgt gaaggaggcg
     1681 cactcgatct tcttcaggcc cgtttccgac agtttgttga tgtaatggat tttatcttca
     1741 gtggacaggc cgttgcatat ctcgggaaag ccgtcccgcg gggacacttc cacgatggtc
     1801 atcgtatttt ctttttttct ggcggatttt cccgccgcat tggcatttgg cataaccgcc
     1861 ccaggccgga gaaagttttt ttaaacatgt tgcataattc attccagccg gccggatctt
     1921 tttattccaa tccgtattcc ttcaatttca aatagaaggt gcgccggctg atcccaaggg
     1981 cccggatggc ttccgaccgg tttgaagaag cggctagcgc ctcggtgatg agccttcgct
     2041 ccaggtcccg gacggcctgg gtcagattga gagaggcagc cccgctttca gcggcaggcg
     2101 gcgcggctcc gtcccgactg cgagattccg aaaccaggaa attgggcagg gaaccggcat
     2161 cgatgacggg cgactgcgtc atgttcagcg cgtagctcac aacgcctttg agctcccgga
     2221 cgtttcccgg ccagcggtac gcatgcagca tcgcgagcgc ttcgggcgaa aaccggtatt
     2281 tctgtttctg cgcggcgccg ctcgccttca ggaaatgctc caccagcaga tcgatgtctc
     2341 ctcttctctc gcgcagcgga ggcagcggga tcgtgaaggt attgaggcgg aaaaaaagat
     2401 cttcgcggaa tttcttctgc ccgatcagat ccgccagatc ctgattggtc gaggagatga
     2461 cccggacgtc caccctgacg ttcctgattc caccgactct tttaaacgtt ccggattcca
     2521 ggaccctgag cagtttggcc tggacggaca gggaggaatc tccaatttcg tcgagcagaa
     2581 tggttccctc gtgcgcctcg tcgaaatagc ctttgcgtcc ctcggtccgc gcgccggtaa
     2641 acgcgccttt ttcatagccg aaaagctcgc tttcaatcag ggaatcggga atggcggcgc
     2701 agttcacgtc gacgagctgc ttgtttttgc ggcggctggt ctggtgaatg gcgcgcgcca
     2761 ggatttcctt gcccgtgccg ctttccccga tgatcagcac ggaaatatcc gattgggccg
     2821 cccgcccggc gctcaccagc gccctgcgaa aggcttcatc gtcgccgatc agttcgtcaa
     2881 acgcgcttcc ggccaacgtg gcatccgcat tcgtcgggga ttcttctttt ccgcgcggtt
     2941 gagccgcttg cgattttttg gttccagccg tcatgggttc ggttttcttt cctgcatgtc
     3001 tatccggtat acaaatttcg ttctgaatgt atataaatca tacatccctg tcaaacagtt
     3061 tttaccgcgc gcggatcagc gactgcgccg ctattgcctt ttttcactgg tatggtattt
     3121 gctgtattca acggaaaaca atctgcaaca aaaggagagt acttatggca gaaatcatcg
     3181 gattttcaaa agaaggcccc gtcggcatca tcacggtcag caacccgccg gtgaacgccc
     3241 tgtcgcaggc ggttcgctcg ggaatcaaag cggggatcga gaagggcatt gcggatgccg
     3301 ccgtttcggc aatggtgatc atctgcgagg gccggacgtt tatcgcgggc gcggacattc
     3361 gggaattcgg ccgcccgccg atggagccgc acctgccgga cgtctgccag ttcattgaag
     3421 attctccaaa gccggttatt gcggcgattc acggaacggc cctgggcgga ggcctggaaa
     3481 tcgctctttc ctgtcatttc cgcattgcgc ttgcctccgc gaaagtaggt cttcccgaag
     3541 tcaaactggg gcttctgccc ggcgcgggcg gaacgcagcg gctgccgcgt gtggcaggcg
     3601 tccaggcggc gctggagatg atcacctccg gaacgccggt ggcggcggcc agggccaaag
     3661 ccatgggcat tctggatgaa gtcgttgaag gcgatctgaa agcggcggcg cttgcctttg
     3721 ccaaaaaagt cgttttggag aaaagcccca tacgcaaagt aagccagctt caagccaaag
     3781 ccgattctcc cagtgttttt gaagacttcg ccaaagccaa cgcccgcaag ttccgcggtt
     3841 tcatggcgcc tttcaagtgc attgaagccg tcaaggccgc cacggaactg cctttcccgg
     3901 aaggcatgaa acgggaaagg gaaatattcg tagagctgca cgattccgat cagtccaagg
     3961 cccagcggca tgttttcttt gccgagcgtg aagtcgtccg cattcccgga ctgcccgacg
     4021 atcagccgac ccgcgagatt aaatccgttg gcgtcctggg cgcaggcaca atgggcggcg
     4081 gcattgccat gtgctttgcc aatgcgggca tccccgtcat gctgctggac gtcaagcagg
     4141 aatttatcga ccggggcatg gccgttatta aaaagaatta cgccaacacc gtcgccaagg
     4201 gctcgctcaa gcaggaggtc atggaaaaac gcctggcgct gatccagccc acgctggcct
     4261 atgaagacct gaaagatgtg gacctcgtca tcgaagcggt ctttgaagac atggccgtca
     4321 agaaggaggt cttcacgaag ctcgacgcgg tttgcaaacc cggcgcgatt ctggcctcga
     4381 acacctccta cctcaacgtc gatgaaatcg ccgccatcac agggcggccg caggatgtca
     4441 tgggcatgca tttcttcagc cccgccaacg tcatgcggct gctggaaaac gtccggggcg
     4501 cgaagacttc ccccgaggtt tacgccacgg ccatgaagac cggaaaaaag atcggcaagg
     4561 tgccggtcct ggtcggcgtc tgcgacggat tcgtcggaaa ccggatgctc gccaagcgca
     4621 cacgcgaatg cggcttcatg ctggaggaag gagcgcttcc ctggcagatc gaccgggcga
     4681 tctacaactt cggcttcccg atgggcccct accgaatggg cgacatggcg ggcctcgaca
     4741 tcggctggcg gaaccgcaag gccaagttcg gctcgctcac gccgcgcgaa caaaagaaca
     4801 acctcctgga caaaatctgc gaaatggggc gctacggaca gaagacgggt tcaggcttct
     4861 acaagtacga tgagaagcgc aacgcgacgc ccgatccgat gattgaagaa ctcatcatca
     4921 accactcgaa ggaagtcggc atcgccaggc ggaccctcac ggatcaggaa attatcgaac
     4981 gcgcgattta ttcgatgatc aacgaaggcg cgaagattct ggaggaaggg atcgccgcga
     5041 ggcccctgga catcgacgtg gtctggcttt atggatacgg ctttccggtg tacctgggcg
     5101 gcccgatgtt ctatgctgat acggtaggtt tgaaaaaagt ctatgacgca gtcctgaagt
     5161 accgggattc ggtgggagcc gaatactgga cgcccgcgcc attgctggaa aagctggcca
     5221 atgaaggaaa aggattttac tccaaataaa aattcggaac ggggaaaagg gaggggatga
     5281 tgaagtatca ggaggtttac gcgcattcga tttctgagcc ggacgccttt tggggcaaag
     5341 ccggggaagc catagagtgg acgaggaggt gggacaaagt tctggatgac agcggcaagc
     5401 ccttttaccg gtggtttgca ggcggagagc ttaacacctg ctacaatgca ctggactgcc
     5461 atgtagaaaa cgggcggaaa gaccaaacgg ccatcatcta cgacagcccc gtcaccgaca
     5521 ccgtccggaa gatcacttac ggcc
//
LOCUS       DAOSIM010000132         5523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_13220_length_5523_cov_6.942575, whole genome shotgun sequence.
ACCESSION   DAOSIM010000132 DAOSIM010000000
VERSION     DAOSIM010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13220_length_5523_cov_6.942575"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /gene="gatB"
                     /locus_tag="PLR47_06665"
     CDS             <1..893
                     /gene="gatB"
                     /locus_tag="PLR47_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOG12664.1"
                     /translation="ICDGNMEEGSFRCDANVSIRPKGQKEFGTRTELKNMNSFRNVQR
                     ALEYEVKRQQYLVENGEKIIQETRLWDDAQGATNPMRSKEEAHDYRYFPDPDLVPVVV
                     DEAWVAEIRAGLPELPQARRERFVNEYQIPAYDAGVLTSEKALAEYYEEVVKLCGRPK
                     IAANWVMGDVLKFLNEDKRSIRECPIAARSLADMIGLIEEGTISGKMAKEVIADMYAT
                     GKTPRGIIEKKGLVQITDEGALAKTIAAIIEANPGQLADYRGGKEKLFGFFVGQVMKA
                     TQGKANPKLVNDLLRKMLAE"
     gene            890..>1498
                     /locus_tag="PLR47_06670"
     CDS             890..>1498
                     /locus_tag="PLR47_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOG12665.1"
                     /translation="MIRTLFWKNNTVVMIDQNALPQTECRIVCTDYRQVIAAIRNLAV
                     RGAPAIGVAAAMGAALGALALPPQPTKAFREKFHSICDEIAAARPTARNLFWALERMK
                     KRLDEVIRAGKSNPTAVLAQEARRICAEDIAINRQMGRQGSVLLSDGDQVLTHCNAGA
                     LASAGYGTALGVIRAAHAGGKKLHVYVDETRPVLQGARLTAWE"
     assembly_gap    1499..1508
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1509..1944
                     /locus_tag="PLR47_06675"
     CDS             <1509..1944
                     /locus_tag="PLR47_06675"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOG12666.1"
                     /translation="MKKEKIPATLICDNMAGSLMHQGKIDKVIVGADRIAANGDTANK
                     IGTYPLAVLARVHRIPFYIAAPVSTFDFSIKTGEEIPIEERRDDEVTAFRGVPAAPKG
                     IKVYNPAFDVTPARLITAIVTEKGILAPPLPAAISRLQKGRI"
     gene            1941..2555
                     /locus_tag="PLR47_06680"
     CDS             1941..2555
                     /locus_tag="PLR47_06680"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG12667.1"
                     /translation="MKLLLFDFDGVLVDSLDVYEKTVTDCLNAIGRPLARGREEFLEL
                     FEGNFYQSLEDKGVDMKKFSDASVDILAKVNYADMKPFHAIRPVLRELKNNHPLIIIS
                     SNDAPTIKEALRLYDFDGIFDDILGCDFMLSKKDKILHVIQKYNVTLQDIYYIGDTTG
                     DMKEGKQAGVKTVAVTWGWHTREKLAATAPDYLFEQPEELLKLN"
     gene            complement(2564..3307)
                     /locus_tag="PLR47_06685"
     CDS             complement(2564..3307)
                     /locus_tag="PLR47_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HOG12668.1"
                     /translation="MIENIKQDITAFLKPQVTELGFASADRFAEAPEKHHPGRICKNA
                     ETVIVFGITVPRGMLKSPDYNLYALHRSYHSAYMRIDEISLALCNYIESLGKCLAVPV
                     PSYAPMVFHQFEPWGILSLKHAAVNAGLGSFGRSGQTYHPRHGALLRLGAVVTDAVIE
                     ADPMLPADPCPPNCTACSKVCPSRAFDTEGRFNKMTCLGYAIKHAIYPLALSTEAGLK
                     NIERVINTAGHNYWIGCDECLKVCPLNRG"
     gene            complement(3372..4673)
                     /gene="larA"
                     /locus_tag="PLR47_06690"
     CDS             complement(3372..4673)
                     /gene="larA"
                     /locus_tag="PLR47_06690"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOG12669.1"
                     /translation="MKAEIRYHNTLLNVEFPDTSRIYLSSYDTPSASAKELALEAVRN
                     PIGSESLVDSIKKRPHSNVVIAVSDITRPIPYRDFLPALLDEIASAGINRKSITILIA
                     TGMHRPSTAEERLFMFGKELAESYAIVDHDAQGEMAVLSGKSHSGAPVKLNSIFAKAD
                     YKMVIGLVEPHFMAGFSGGRKTICPGLSSLDTIKQFHGYEFLSHPNAFSSRLDKNPCH
                     EEALSVAKLAGADFSLQLVVNHEKKIVKAFGGDLFESHAVACRYASQKACPVVEAEAD
                     VVVTGCGGYPLDTTFYQCVKALVNALPCVKNGGTMIAAGGCGEGVGSQTFETMLRKYQ
                     NDFSGFIRDIKKTSDVVKDQWQIQMQARVYEKIELNKIHFLTHGIPPGHSSFLGVNIV
                     EAASGRVAETLQKTIDDLAGKGCSFAVIPEGPYCAPQSRGT"
     gene            complement(4685..5341)
                     /locus_tag="PLR47_06695"
     CDS             complement(4685..5341)
                     /locus_tag="PLR47_06695"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HOG12670.1"
                     /translation="MNTTVILASAVVLLCGLLLAEKKENLKGILLTKPLLSALFIATA
                     LLQPHSADPYFYLILAGLVLCFAGDICLIFFFNRKVFTAGLAFFLTGHILYVLAFFIT
                     AGFTTGAWISLAVMSVISFFVFTRLRPHLGSMVGPVIAYITIISVMVVAASSLTARQD
                     FSLQARTLVMAGSILFYVSDLFVARHRFVAKKIVNRYAGLPMYYAAQFMLAFSVGMIP
                     "
BASE COUNT         1299 a         1437 c         1588 g         1189 t
ORIGIN      
        1 aaatctgcga cggcaacatg gaagaaggct ctttccgctg cgacgccaac gtgtcgatcc
       61 gccccaaggg ccagaaggaa ttcggcacgc gcacggaact gaaaaacatg aactccttcc
      121 gcaacgtgca gcgggctctg gaatatgaag tcaagcgcca gcagtatctg gtggaaaacg
      181 gcgagaaaat tattcaggag acgcgcctgt gggacgacgc gcagggggcg accaacccca
      241 tgcgctcgaa ggaagaagcg cacgactacc gctatttccc cgatcccgac ctggtgccgg
      301 tggttgtgga tgaagcctgg gtggctgaaa tccgcgccgg cttgccggag ctgccgcagg
      361 ccaggcgcga gcggtttgtg aacgagtatc aaattcccgc ctacgacgcg ggcgtgctca
      421 cttcggaaaa ggcgctggcg gaatattacg aagaggtggt gaagctctgc ggcaggccca
      481 agatcgccgc caactgggtc atgggcgatg ttctgaagtt tctcaatgag gacaagcgga
      541 gcatccggga atgcccgatt gcagcacgtt cgctcgcgga catgatcggc ctcattgaag
      601 aagggaccat cagcggcaag atggccaagg aagtgattgc cgacatgtat gcgaccggca
      661 agacgccgcg gggaatcatt gaaaaaaagg gcctggtgca gattaccgat gaaggggcgc
      721 tggcaaagac cattgcggcg atcatcgagg ccaaccccgg ccagctcgcg gattatcggg
      781 gcggcaagga aaagctcttc ggatttttcg tcggccaggt gatgaaggcc acgcagggca
      841 aggccaatcc gaagcttgtc aacgatctgc tgagaaagat gctggccgaa tgatcaggac
      901 cctgttctgg aaaaacaaca ccgtcgtgat gatcgatcag aacgctctgc cgcagacgga
      961 gtgtcgaatc gtatgtacgg actaccggca ggtcattgcc gccattcgga atctggcggt
     1021 gcggggcgcg cccgcaatcg gcgtcgccgc cgccatgggt gcggcgctgg gcgcacttgc
     1081 gctcccgcct cagccaacga aggcgttccg ggaaaagttt cattccattt gcgatgaaat
     1141 cgccgcggcg cggcccacgg cgcgcaatct tttctgggcg ctggagcgga tgaaaaagcg
     1201 cctggacgaa gtcatccgtg cgggaaagag caatccgacc gcggtactgg cgcaagaggc
     1261 aaggcgaatt tgcgccgaag acattgcgat caatcggcag atgggccgtc aaggaagcgt
     1321 tcttttatcc gacggcgacc aggtgcttac gcactgcaac gccggcgcgc tggcgagcgc
     1381 cggctacggc acggcgctgg gcgtgattcg cgcggcgcac gccgggggaa agaaactgca
     1441 cgtctatgtg gatgaaacgc gtccggttct gcagggggca aggcttaccg cctgggaann
     1501 nnnnnnnnaa tgaagaagga aaaaattccc gccacgctga tttgcgacaa catggcggga
     1561 tccctgatgc atcagggaaa aatcgataag gtcattgtcg gcgcggaccg gattgcggcc
     1621 aacggagaca cagccaataa aatcgggact tacccgctgg ccgttctggc gcgggttcac
     1681 cggattcctt tttatattgc cgctccggtt tccacgtttg atttttcgat caaaacggga
     1741 gaggaaattc ccatcgaaga aagacgagac gatgaagtga cggcctttcg cggcgtaccc
     1801 gccgcgccaa aaggcataaa ggtgtacaac cccgcatttg acgtcacgcc ggcgcggctg
     1861 attacggcca tcgtcacgga aaaaggaatt ctggcgccgc ccctgccggc ggccatttcc
     1921 cgccttcaaa aaggaagaat atgaagctgc tgctttttga ttttgacggc gttttagtag
     1981 attcgcttga tgtttatgaa aaaacggtga ccgactgcct caacgcgatc ggccggccgc
     2041 tcgcgcgcgg ccgggaagag tttctggagc tttttgaagg caacttctac cagtcgctcg
     2101 aagacaaggg cgtggacatg aaaaagtttt cagacgcgtc cgtggacatt ctggcaaaag
     2161 tcaactacgc ggacatgaag ccttttcacg ccatccggcc ggtgctgcgg gagctgaaga
     2221 acaaccatcc cctgatcatc atttcgtcca acgacgcgcc gacgatcaag gaagctctgc
     2281 gcctgtatga ttttgacgga attttcgacg atattctggg gtgcgatttc atgctcagca
     2341 aaaaggacaa gatccttcat gtcattcaaa aatacaacgt cacgctgcag gatatttact
     2401 atatcggcga cacgaccggc gatatgaaag aagggaaaca ggccggcgtg aaaaccgtag
     2461 ccgtgacctg gggatggcac acgcgggaaa aactggcagc gaccgcgccg gactatcttt
     2521 ttgagcagcc ggaagagctg ctgaagctga actgacgctt tcgtcatccc ctgttcaaag
     2581 gacacacctt caggcattca tcacagccga tccagtaatt atgcccggcg gtattgatca
     2641 ccctctcgat atttttcaat ccggcttcgg tcgagagggc gagaggatag atggcatgct
     2701 tgatcgcgta tcccaggcag gtcattttgt tgaacctgcc ctccgtatca aaagccctgg
     2761 acgggcagac tttggagcag gccgtgcagt ttggcgggca gggatcggca ggcagcatcg
     2821 ggtccgcttc gatcaccgcg tccgtcacca cggcgcccag cctcagcagt gcgccgtgcc
     2881 tcgggtgata ggtctggccg ctgcgcccga agcttcccag gccggcgttg accgccgcgt
     2941 gcttcagcga cagaatcccc cacggctcaa actgatgaaa aaccatcggg gcatagctgg
     3001 gaaccggcac ggccaggcac ttccccagcg attcgatata gttgcacagc gcgagggata
     3061 tttcgtcaat gcgcatgtag gcgctgtggt agcttcggtg cagggcgtat agattgtaat
     3121 ccggtgattt gagcattccg cgggggacgg taatcccgaa aacaatcacg gtttcggcat
     3181 ttttgcagat gcgcccggga tggtgcttct cgggggcttc ggcaaagcga tccgccgatg
     3241 caaagccaag ctccgtgacc tgcggtttca gaaaagctgt aatgtcctgc ttaatgtttt
     3301 caatcatcgt ccaccatcct tttttaaacc ggaaaccgtc aataaatttt tattggttaa
     3361 aaaattaagc gttatgtccc cctgctctgt ggtgcacaat agggcccttc cggaatcacg
     3421 gcgaaagagc atccctttcc ggccagatcg tcaatcgttt tttgcagcgt ttcggcgact
     3481 ctgcctgaag cggcctcgac aatatttact cccagaaagg agctgtggcc gggaggaatg
     3541 ccatgcgtca gaaagtgaat tttattcagt tcgattttct cataaaccct ggcctgcatc
     3601 tgtatctgcc actggtcttt tacaacgtcg ctggtctttt tgatgtcgcg gataaagccg
     3661 gagaagtcgt tttggtactt gcggagcata gtttcaaacg tctgtgatcc tacaccttcc
     3721 ccgcatccgc ccgcggcaat cattgttcct ccgtttttta cacacggcag ggcgtttaca
     3781 agtgctttga cgcattgata aaaagtggtg tccaggggat accccccgca gccggtaacc
     3841 actacatccg cctctgcttc gacgaccgga caggcctttt ggcttgcgta acggcaggcg
     3901 acggcgtggc tttcaaagag gtccccgcca aaggccttga cgatcttttt ttcatggttc
     3961 acgaccagtt ggaggctgaa atcggcgccg gcaagtttgg ccacggaaag cgcctcttca
     4021 tggcagggat ttttatcaag ccgggatgaa aaggcgtttg gatgcgagag gaattcatac
     4081 ccgtgaaact gtttaatcgt atccagggag gaaagcccgg ggcagatggt tttgcgcccg
     4141 cctgaaaatc ctgccataaa atggggctcg acaagcccga tgaccatttt ataatcggcc
     4201 ttcgcaaaaa tgctgttgag ctttaccggg gctccggaat ggcttttgcc tgaaaggacg
     4261 gccatttcgc cttgtgcatc gtgatccaca atggcatagc tttcggccag ctctttgccg
     4321 aacatgaaga gccgctcttc ggctgtgctg ggcctgtgca tgccggtggc gatcagaatg
     4381 gtaatgcttt tccgattgat tcccgcagat gcgatttcat ccagcagtgc gggcaaaaaa
     4441 tcccggtagg ggatcggccg ggtaatgtcc gataccgcaa tgaccacatt gctgtgaggt
     4501 ctttttttga tcgagtccac aagactttcc gagccgatcg gatttcgaac cgcctccagg
     4561 gcaagttctt ttgcggaagc ggagggagta tcgtaggatg aaagatagat ccggcttgtg
     4621 tcgggaaatt ccacattgag cagggtatta tgatatctga tttccgcttt cataagatct
     4681 ttccttatgg aatcattccc actgaaaagg ccagcatgaa ctgcgcggca tagtacatcg
     4741 gcagacccgc ataacggttg acgatttttt ttgccacgaa tctgtgtcgt gccacaaaga
     4801 ggtctgaaac atagaagagg atcgaccctg ccatgaccag tgtccgggcc tgaaggctga
     4861 agtcttgacg ggcggttagg gaagaagctg ccacaaccat gacggaaatg atggtgatat
     4921 acgcgatcac cgggcccacc atggaaccga gatgggggcg cagcctggta aatacgaaga
     4981 aggaaatgac ggacattacg gcaagggaga tccatgcgcc ggttgtaaag ccggcggtga
     5041 tgaaaaacgc cagaacgtat aaaatgtgtc ctgtgaggaa gaatgccagg ccggcggtaa
     5101 agactttgcg gttaaagaaa aagatcaggc agatatcgcc cgcaaagcac aagaccagac
     5161 ctgccagtat gagataaaaa tagggatccg cgctatgggg ctggagcaga gccgttgcga
     5221 tgaagagcgc tgacaggagc ggcttggtca gaaggatgcc cttgaggttt tctttttttt
     5281 ccgccaaaag aagaccgcag agcaaaacga cggctgatgc caggatgact gttgtattca
     5341 ttattttctc tccataaaat tgtacaggtg ctttcctgcg tctggcgctg ttgcggttgc
     5401 cctatcgcgc agctttcctt tttcagcaat caacacatca tcatttatta ctcaagtcat
     5461 ttttgcggac cggcgccaat cttaaatttg cctttgaaat actttgccct gatctcggtc
     5521 act
//
LOCUS       DAOSIM010000133         5512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_13254_length_5512_cov_8.086311, whole genome shotgun sequence.
ACCESSION   DAOSIM010000133 DAOSIM010000000
VERSION     DAOSIM010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13254_length_5512_cov_8.086311"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PLR47_06700"
     CDS             complement(<1..254)
                     /locus_tag="PLR47_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12671.1"
                     /translation="MTKIKAAGTALLNQWRLLAGLKYFWPVFLAAGLAFCIAADVYDF
                     VDLYLQLIIIYVGINIILTASLNLINGYMGEFSVGHAGFMA"
     gene            complement(257..1132)
                     /locus_tag="PLR47_06705"
     CDS             complement(257..1132)
                     /locus_tag="PLR47_06705"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOG12672.1"
                     /translation="MAYLFQQALNALQLGSIYALIALGYTMVYGILTMINFAHGDLFM
                     VGAFFCFLLAVYLKLSFVPTLLLSMLGVACLGVIIERLAYKPLRAAPRVSAIITALGV
                     GLFLENFTLALSPYPKHIPPLLTNTTWAIGSLSISSLQVLVIALSLILMLILDFVVQR
                     TKAGMAMRAISWDKTIVPLMGVPVNKIISLTFAIGTGLGGAAGVMYGLAYPVIDPYMG
                     IMVGWKAFICAVVGGIGNVRGAMIGGFILGAVEILVAAFFPSTYRDFVAFTLLLVLLI
                     LRPYGILGKPQPQKV"
     gene            complement(1253..2419)
                     /locus_tag="PLR47_06710"
     CDS             complement(1253..2419)
                     /locus_tag="PLR47_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG12673.1"
                     /translation="MRKTYIWIALLVIGSLCIFGCAKKDSDAVRIGVIAELTGDIPAV
                     GASCKNAAELAVREINDGGGILLGDKKYPVELIIEDNAGKADQSASSAQKLITQQKVT
                     AIVGPNASRYALPAAEIAESGKTVLITPWSTAPKATLNSKTNASKQYVFRACFIDPFQ
                     GGVIAKFTLDNLKLKKTAVLYDVASEYNKGIAEIFREVYEKNGGAIVAFETYTTNDKD
                     FSSQLTKIKKANPDIIFLPNYYSEVPLQIQQAKRLGITVPFIGSDSWGSEELLKLCGK
                     ECEGYYFSTHYAADSASDATKKFIEAYKAKYGTTPDDVAALTYDSFGLLLQAIKTAGK
                     NDRQAVRDALAKIPLYQGVTGSMQFKEGSGDPIKSAVILQIKDGKFAWFADAKP"
     gene            2612..3433
                     /gene="xth"
                     /locus_tag="PLR47_06715"
     CDS             2612..3433
                     /gene="xth"
                     /locus_tag="PLR47_06715"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOG12674.1"
                     /translation="MVRTGENGKAFSILKIATYNVNSIRSRLHIVLPWLESHQPDIFC
                     MQETKVEDAKFPLAEFEAAGYTVTFRGGKQYNGVAVASRIKPQKTSFGLAGDPSDPDR
                     LAVAHFGDLVVLNAYVPQGQEVDKPQFAYKLAWLGRLKKYLLTNFRPDQKLVLCGDFN
                     VAPQPMDVHDPKRILGHVSFNPEVWKAYEDVLSWGLVDVFRHHHPDEPGQYTFFDYRV
                     KDSVGRNLGWRVDHVLATRKTADQSSGCVIDRATRLAEKPSDHAVIYAEWEGKLR"
     gene            3430..4005
                     /locus_tag="PLR47_06720"
     CDS             3430..4005
                     /locus_tag="PLR47_06720"
                     /inference="COORDINATES: protein motif:HMM:NF016243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOG12675.1"
                     /translation="MRKKEDIHRINEDLAARFAKVEATIASAPTMAGLFEALVEGIER
                     EFAVPFVWLTFVDRPGVDLWIDDAGASEILKSRFSVVSAELLGKILSGGLHPVLSNQN
                     LQPFYKLMPPSQKYFVRSIAVVPFAQDGQVIGAWNNGDADADRYSPDMDTALLSSLAD
                     SVSLRLTELAAEKNAASAPEKSAEQPGGFHG"
     gene            3998..4348
                     /locus_tag="PLR47_06725"
     CDS             3998..4348
                     /locus_tag="PLR47_06725"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HOG12676.1"
                     /translation="MVDEWIQEIKKKCPPDMLGMILVHNGVVRATSKKGGPVARMKLS
                     YSRAALDEAVRRFKEREGIAEIRAWINEGTLGVGDDIMVVLVAGRFRTDVLPVFQELI
                     SLIKTEIVREDETS"
     gene            4495..>5512
                     /locus_tag="PLR47_06730"
     CDS             4495..>5512
                     /locus_tag="PLR47_06730"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOG12677.1"
                     /translation="MDTMTEFREVADQPYGYLQKLKDQTGKKMVGYVCSYTPEEILLA
                     AGAHPVRLFGTKQQIHLADSHLQSYSCSIVRGVLEEGLSGRIGFLDGMVFPHTCDSIQ
                     RLSDIWRLNIPFGFHSDLVLPVKLNTPSARDYMTDVLRKFQNDVGRALGVDITDDALQ
                     KAIGTMNAIRQSIRDIYEIRSQYPRLMSGADLYTLVRASMMMDRERLAGMLSETVVKM
                     KAKAAGEKPDPSLKRIVLAGGMCNHPDIYPMIEEAGGAVVWDDFCTGARYFAELVAGG
                     DDPAAGIAERLIRRVVCPAKHAGLDRRAKHILRLVKEQNARGVIFLLFKFCDPHAFDY
                     PDIRK"
BASE COUNT         1236 a         1434 c         1633 g         1209 t
ORIGIN      
        1 gccatgaatc ccgcgtgccc cacggagaat tctcccatgt agccgttgat caggttcagg
       61 ctggcggtga gaatgatgtt gatgcccaca taaatgataa tgagctgcag gtataaatcc
      121 acaaagtcat aaacgtcggc ggcgatgcaa aaggcaagtc ccgcagccag aaagaccggc
      181 cagaaatatt tcaggccggc aagaaggcgc cattgattga gcaatgctgt gccggcggct
      241 tttatctttg tcatgctcat accttttgcg gctggggttt gcccagaatg ccgtagggcc
      301 gcagaatcaa aagaaccaat agcagcgtga aagcgacgaa gtcgcggtac gtggacggaa
      361 aaaacgccgc cacgagaatt tccaccgcgc ccagaatgaa accgccgatc atggcgccgc
      421 gcacgttgcc gatgccgccc accaccgcgc agataaaggc tttccagccg accatgatgc
      481 ccatgtaggg atcaatcacc gggtaggcca gcccgtacat gacgcccgcc gctccgccca
      541 gacccgtgcc gatggcgaag gtgagcgaaa tgattttatt gaccggcacg cccatcaggg
      601 gaacgatcgt cttgtcccag gaaatggcgc gcatggccat gcccgctttg gtgcgctgga
      661 cgacaaaatc cagaatcagc atcagaatca gcgaaagcgc gattaccagc acctgcaaag
      721 aagaaatgga caacgatccg atcgcccatg tggtgttggt cagaagcggc gggatgtgct
      781 tgggatacgg ggaaagggcc agcgtgaaat tttccagaaa caatcccacg cccagcgcgg
      841 tgatgatggc ggagacgcgc ggcgcggcgc gcagcggttt ataggccagt ctttcgatga
      901 tgacgcccag acaggccact cccagcatcg ataaaagcag ggtcgggaca aacgacagct
      961 tcagataaac agccagcagg aagcagaaaa acgctccgac catgaacaga tcgccgtggg
     1021 cgaagttgat catcgtcagg atgccgtaaa ccatcgtgta gcccagcgcg atgagcgcat
     1081 agatgctgcc cagctgaagg gcgtttaggg cttgctgaaa aagataggcc ataccggaac
     1141 cttccccgcc ccctgcggga ggggatcgag gggaggggag ttttatttgg atcacccttc
     1201 ctcgatccct cccatcaagg gaaggatcgc tgatgaagga tgaaaatact ttctatggtt
     1261 ttgcgtccgc aaaccacgca aactttccgt ctttaatctg cagaatgacc gcgcttttga
     1321 tcgggtcgcc ggagccttct ttaaactgca tgctgccggt taccccctgg tagagaggaa
     1381 tttttgccag cgcgtcgcgc acggcctgcc ggtcgttttt accggcggtt ttaatggcct
     1441 gcaggaggag tccgaaggaa tcatacgtca gggcggctac atcgtcgggg gtcgtcccgt
     1501 atttggcttt gtaggcctca atgaattttt ttgtcgcgtc gcttgccgag tcggccgcgt
     1561 aatgcgtgct gaaataatag ccttcgcatt ctttgccgca gagcttgagc agctcttcgg
     1621 agccccagga atccgagccg ataaacggga cggtgattcc cagacgcttg gcctgctgga
     1681 tttgcagcgg aacttcgctg taatagttcg gcaggaagat aatgtccggg tttgcttttt
     1741 tgatcttggt gagctgcgat gagaaatcct tgtcattggt cgtatacgtt tcaaaagcga
     1801 cgattgcgcc gccgtttttc tcgtacacct ctcggaaaat ctcggcgatg cctttgttgt
     1861 attcggaagc cacatcgtac agcaccgctg tttttttcag ctttaaatta tccagcgtga
     1921 atttggcgat cacgcctccc tgaaagggat cgataaagca ggcgcgaaaa acgtattgtt
     1981 tggaggcatt ggtcttggaa ttcagcgtgg ccttgggtgc ggtggaccag ggggtaatca
     2041 aaacggtttt gcccgactcg gcgatttccg ccgccggcaa cgcgtagcgg ctggcgttgg
     2101 ggccgacaat ggctgttact ttctgctggg tgatcagctt ctgcgcggag gaggcggact
     2161 gatcggcttt tccggcattg tcttcgatga tcagttccac cggatatttc ttatccccca
     2221 gaagaatgcc gccgccgtcg ttgatttccc ggaccgcaag ctccgcggcg tttttgcagg
     2281 aggcgccgac cgccggaatg tcgccggtca gctcggcgat gacgccgatt ctcaccgcgt
     2341 cggaatcctt tttggcacag ccgaaaatac acagtgagcc aatcaccaaa agagcaatcc
     2401 atatataagt ttttctcatg gggaatctcc cgtccggaac taaaaatttt gaccacagct
     2461 tataagggaa ggacgaagca gtgtcaatgg aaaagaaggt cgttgacgta ccggacgacg
     2521 gccggcgagg cggtgagtcc cggcgatgat attcagggcg gcagctccgt ttcggcggcc
     2581 ggcgaaaaaa tcttgagcgg tgcggccggt cgtggtaaga accggagaaa acggaaaggc
     2641 cttttcaata ttgaagattg ccacgtacaa cgtcaattcg attcgttccc gtctgcatat
     2701 cgtgctgccc tggcttgaaa gccatcagcc ggatattttc tgcatgcagg aaaccaaggt
     2761 cgaggacgcc aaatttcccc tcgctgaatt tgaggcggcg ggatacacgg taaccttccg
     2821 cggcggcaaa caatacaatg gcgtggccgt cgcctcccgc atcaagccgc aaaagacttc
     2881 cttcggactg gccggtgatc cgtcagaccc ggaccgcctg gcggttgcgc atttcgggga
     2941 tctggttgtt ttaaacgcct atgtgccgca ggggcaggaa gtggataaac cccagtttgc
     3001 ctataagctg gcgtggctgg gaaggctgaa aaaatatctt cttacgaact tccgtcccga
     3061 tcaaaagctg gtgttgtgcg gtgatttcaa tgtggcgccc caaccgatgg acgttcatga
     3121 tcccaagcgc attctggggc atgtttcctt caatccggag gtctggaagg cttacgagga
     3181 tgttttgtcc tgggggctcg tggatgtttt ccgtcatcat catcccgacg agccggggca
     3241 gtacactttt tttgattacc gcgtgaagga ttccgtcggg cgaaacctgg gctggcgcgt
     3301 ggatcatgtt ctggccacaa ggaaaacagc ggatcaatcg agcggctgcg tcattgaccg
     3361 ggccacgcgt cttgcggaaa agccgtccga tcatgcggtg atttacgccg agtgggaagg
     3421 gaagcttcga tgaggaaaaa agaggatatt catcgcatca atgaagatct tgcggcgcgc
     3481 tttgcgaaag tggaagcaac gatcgcgtcc gcccccacca tggccggact ttttgaggcg
     3541 ctggtggaag gaatcgaaag ggaattcgcc gttccttttg tctggctgac gtttgtggat
     3601 cggccggggg tggatttatg gatagatgac gcgggcgcat cggagattct gaaaagcagg
     3661 tttagcgtcg tttccgcgga gcttctggga aaaattcttt caggcgggct gcatcccgtt
     3721 ctctccaatc aaaatcttca gcctttttac aagctgatgc cgcccagtca aaaatatttt
     3781 gtccggtcaa tcgccgtggt tccttttgcg caggacggtc aggtgatcgg agcctggaac
     3841 aacggcgatg cggacgcgga ccgctattcg ccggatatgg ataccgcgct gctttcgtca
     3901 ctggccgaca gtgtttccct tcggctgact gaactggccg cggagaaaaa tgccgcatcc
     3961 gctccggaga aaagcgcgga gcagccggga ggttttcatg gttgacgagt ggattcaaga
     4021 gatcaaaaaa aagtgcccgc cggacatgct gggaatgatt ctggtgcaca acggcgttgt
     4081 gcgggccacg tccaaaaaag gcggtccggt ggcgcgcatg aaactttctt acagccgggc
     4141 cgcgctggat gaggcggtgc gccggttcaa agaaagggaa ggcatcgcgg agattcgcgc
     4201 ctggatcaac gaagggacgc tcggcgtcgg cgacgacatt atggtggttt tggtggcggg
     4261 ccggttccgc acggatgttt tgccggtgtt tcaggagctc atttcgctga tcaaaacaga
     4321 aatcgtgagg gaagatgaga cttcatgagc aaggccctca tttgctttgc ccgccgccgg
     4381 tttttggcag gcggcaggca ggaacgcgaa tatagataac acttttgagc ccgaatatgc
     4441 tagcttgcgt tcaccaaacg gaaaagccgc tcttcgggcc gggaaggaaa tgcgatggac
     4501 accatgacgg aattcaggga agttgctgac caaccttacg ggtatctgca aaaactcaaa
     4561 gaccaaaccg gaaagaagat ggtgggctat gtctgttcct acacgccgga ggagattctt
     4621 ctggcggccg gggcccaccc ggtgcgtctt ttcggcacca aacagcagat tcatctggcc
     4681 gattcccatc ttcagtccta cagctgctcg attgtccgcg gcgttctgga ggagggcctt
     4741 tccgggagaa tcggatttct ggacggcatg gttttcccgc acacctgcga ttccattcag
     4801 cggctgtccg acatctggcg tctgaatatt ccgttcggat ttcattcgga cctggtgctg
     4861 cccgtcaagc tcaacacgcc ctccgcacgg gactatatga cggatgtcct gagaaaattc
     4921 caaaacgacg tcggccgcgc gcttggggtg gacattaccg acgacgccct gcaaaaagca
     4981 atcgggacga tgaatgccat tcggcaatcg atcagggata tttacgagat ccgcagccaa
     5041 tacccccgtc tcatgtccgg cgcggattta tacacgctgg tccgggcgtc tatgatgatg
     5101 gaccgggagc gtctcgcggg gatgctttcc gaaacggttg tgaagatgaa ggcaaaagcg
     5161 gccggggaaa agccggaccc ctctctgaag aggattgttc ttgcgggcgg catgtgcaat
     5221 catccggata tttatcccat gattgaagag gcgggcggag ccgttgtctg ggatgatttc
     5281 tgtacggggg cgagatattt tgccgagctg gtcgccggtg gggatgaccc tgcagccggc
     5341 atcgcggagc ggctgatccg gcgcgtggtt tgcccggcca agcatgccgg cctggacagg
     5401 cgcgccaaac atatccttcg cctcgtaaaa gaacaaaacg cccggggcgt catctttttg
     5461 cttttcaaat tctgcgatcc gcacgcgttt gattatcccg acattcggaa aa
//
LOCUS       DAOSIM010000134         5469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_13378_length_5469_cov_6.992612, whole genome shotgun sequence.
ACCESSION   DAOSIM010000134 DAOSIM010000000
VERSION     DAOSIM010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13378_length_5469_cov_6.992612"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="PLR47_06735"
     CDS             <1..579
                     /locus_tag="PLR47_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12678.1"
                     /translation="SNSLKLPLLELAMPSLSVMTGMEKRNFLLMLHSLIHADGKLSLL
                     ELSVQWILEKHLNPSEDLFRTIRKFSFSQVGLDIVTLLSALASAGHTHDKDKAQKAFD
                     AGVARIPELAARKPVLSFQEIMSYAAVNRALQSLTDASFKIKESVVDACAHCAFSDQA
                     VTFEEGELLRVVSLALQCPLPPFIQPPLRDAA"
     gene            651..1988
                     /locus_tag="PLR47_06740"
     CDS             651..1988
                     /locus_tag="PLR47_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOG12679.1"
                     /translation="MSIWQKKSRILITCPKGVVPYLKAEIEALGFPVVQEIDTAVSTE
                     GTLADTMSLNLHLRTAQHVLYQLQIFKALSPGALYERLNAIPWETILHDSGPSAYVCV
                     TSVADHPDVTDSRYVNLRAKDAIVDRIREKTGIRPDSGPERNRAVIHVYWRNDQAMAY
                     IDTSGERLSLRGYRKIPLAAPMQETLAAALIMATQWRGRTPLVNPMCGSGTLAIEAAL
                     IALHRAAGLGRSNYGFLHIRDFPREAWQALRKDARDASLKALPSTIIASDIDPRAVEA
                     ARRNAQTAGVDRLIEFRVCPFEKTPTPGGKGVIVMNPPYGERLEPFKIKTSHPEHRKE
                     IAPGQKVIVRKAADIVHRSGGSTTLQRLEALYEGCGDWFKSIGRGYLGYIFTGNLEMA
                     KKVGLRTRRRMTFYNGEIECRLLEYELYAGSKKHKAAPARPDDADTGAQHGSG"
     gene            complement(1994..2383)
                     /locus_tag="PLR47_06745"
     CDS             complement(1994..2383)
                     /locus_tag="PLR47_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12680.1"
                     /translation="MTDILDYISTWSALKWVVLVLIAGFIGQFGRMTAEAITEKIRLR
                     RAGKNPLTHGPQNPEEPKALSTGAVGENGLPVPVQEMKNLQAQVPVAETPPAAASPVF
                     PPDKKALKAMAKARKKEAKKKEKESLP"
     gene            2579..3670
                     /locus_tag="PLR47_06750"
     CDS             2579..3670
                     /locus_tag="PLR47_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOG12681.1"
                     /translation="MRKAIVKSITLLLIACVSACAPPPLQKKDDKIAAALADPQTSFF
                     EACRRGDIAMVMRLAEEKRIDVNQADDYAWTGLMYAVQNGHTGLAEYFLLQKSQINRQ
                     DGDGLTPLMIAANERHSDMVDLLLAFGADPLLQDRHGYTALMWAIYNKSWDCIPPLLA
                     EGKGLDVQNGFGETALHLALDYHCSDIAGILIRQNANVKFADGRGRTPLHLAAERGDE
                     DNAWLLLSRGADANAGDESGENPLMKSIQSGNPDLALLLLPRTGNLQATNREGRTALH
                     LAVVRDLPDTAAALIRRKADLNSSDEQGFTPLMLAAESGSERMTVLLAERGADLKMKN
                     KEGKTALDLAREKSHGNIATYLERKMGKP"
     gene            3667..4962
                     /locus_tag="PLR47_06755"
     CDS             3667..4962
                     /locus_tag="PLR47_06755"
                     /inference="COORDINATES: protein motif:HMM:NF017808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG12682.1"
                     /translation="MKEQPDLFRWMLAAALTIVLIAGLISCRYLSIPAQDDAVPALLP
                     SKEEKGPTVYNSAVIKGRVTGPGWKGHSTLILACRSADKKNGCGEYAATRENQDAFML
                     YLPEGRYCLIAVTDHNHDGKYEKDEASGAYGLPRQMMEITVREGDLITGVEIRTAAAG
                     ERPVRLPRECRWKIEEPVIRQATGNGDLLKIYHEYFSLENAQTGYWNPSSFMKAFGAH
                     IYLTQAYDSRKIPLLFVHGTEGTPQNWIYCYMRLDRDRYQPWFFYYPSGIRLNLAAAL
                     LQEELRELQEQYGFRKMALAAHSVGGLTARSFLIRRESEERPPFIRLFASFATPWNGF
                     GLADASRVLPHKSIPVWLDLGTHSEFISATMNAKLPPSVRHYLFYGREDKLTGGAEPD
                     SRATAGTAGVYGFDCTHDGILIDRRVFSRFNTILEKEMN"
     gene            4964..>5469
                     /locus_tag="PLR47_06760"
     CDS             4964..>5469
                     /locus_tag="PLR47_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOG12683.1"
                     /translation="MKIIRFLSESGKTLYGAYSKRRPGTAEGIEKEPWSKNITGGGEE
                     KIRKLLAPVAPVNILALGINYRKHGEETALSIPSEPILFLKATSSVTGPDHPIVLPAA
                     GSDQVDYEAELALVIGKTAKNVTPAEAWDCIFGFTCANDVSARDWQFERQKGQWARGK
                     SFDTFCPLG"
BASE COUNT         1213 a         1617 c         1559 g         1080 t
ORIGIN      
        1 tccaacagcc tgaagctgcc tcttctggaa ctggccatgc cgtctttgtc ggtcatgacc
       61 ggcatggaaa aacgaaattt cctgctgatg ctgcactccc tgatccatgc cgacggcaaa
      121 ctgagcctgc tggagctgtc cgttcaatgg attctggaaa agcatttaaa cccttccgaa
      181 gacctgtttc gcaccatcag gaaattttcc ttctcccagg tggggctgga cattgtaacg
      241 cttctttccg ccctggcctc cgccggacac acgcacgata aagacaaagc gcaaaaggcg
      301 tttgacgcag gtgtggccag aatcccggag ctggccgccc ggaaaccggt tctttcgttt
      361 caagaaatca tgtcctacgc tgccgtgaac cgggcgctcc aatcgcttac cgatgcctcc
      421 ttcaagatca aggaatccgt cgtcgatgcc tgcgcgcact gcgccttttc ggatcaagcc
      481 gtcacgttcg aggagggtga actcctgcgc gtcgtctcgc tggcgctgca atgccccctc
      541 ccgccgttta tccagccgcc gctgcgggat gccgcctgat tcgaagaagt ttcgaaaacc
      601 tgaattttta aaagattgcc aagacctatg ggctgtgtta aagcctgcgc atgtccatct
      661 ggcaaaagaa aagcaggata ttgatcacct gtccgaaagg ggttgttcct tacctgaagg
      721 cggaaatcga ggcgctggga tttcccgtcg tgcaggaaat cgacacggcc gtttccaccg
      781 agggaaccct ggcggatacg atgtctttaa acctgcacct gcgcacggcc cagcacgttt
      841 tgtatcaact gcaaatcttc aaggccctct cccccggcgc tctttacgaa cgactgaacg
      901 ccattccctg ggaaacgatt ctgcacgact ccggccccag cgcctatgtt tgcgtcacat
      961 ccgtggccga tcacccggac gttaccgatt cgcgctacgt caacctcagg gccaaggacg
     1021 ccattgttga ccgaatccgc gaaaagacgg gcattcggcc ggattccggg ccggagagaa
     1081 acagggcggt cattcatgtt tactggcgaa acgatcaggc catggcatac attgacacgt
     1141 cgggggaaag actttccctt cgcggctacc gcaagattcc gctggcggcg ccgatgcagg
     1201 aaacgctggc ggcggcgctc atcatggcca cccaatggcg gggccgaacg ccgcttgtca
     1261 atccgatgtg cggcagcggc acccttgcaa tcgaggcggc cctgatcgcg ctccatcggg
     1321 cggcgggact ggggcgcagc aattacggct tcctgcatat ccgggatttc ccgcgggaag
     1381 cgtggcaggc gctgcgcaaa gacgcacgcg acgcgtcatt gaaagctttg ccgtccacca
     1441 ttatcgcatc ggacatcgat ccccgggcgg tggaggccgc gcgccgaaac gcgcaaacgg
     1501 ccggggttga ccgcctcatc gaattcaggg tctgtccctt tgaaaaaaca cccacgccgg
     1561 gcggcaaagg cgttatcgtg atgaatcccc cctacggaga acggctggag ccttttaaaa
     1621 tcaaaacgtc tcatccggag cacaggaagg aaatcgcgcc ggggcagaaa gtcatcgtcc
     1681 gcaaggccgc cgacatcgtt caccgaagcg gcggttccac cacccttcag cgcctggaag
     1741 cgctttacga aggctgcggc gactggttca aaagcatcgg ccggggttat ctcggctata
     1801 tttttacggg caatctggag atggccaaaa aagtggggct gcgaaccagg cgccgaatga
     1861 ctttttacaa cggggaaatc gaatgccgtc tgctggagta tgaactctac gcgggaagca
     1921 aaaaacacaa ggccgcaccc gctcggccgg atgacgccga caccggcgct cagcatggct
     1981 ccggatagtc gcgttacggc agggattctt tttctttttt cttggcttct ttttttcggg
     2041 ctttggccat cgccttcagc gcttttttat ccggcggaaa cacgggcgaa gcggcagcgg
     2101 ggggcgtttc ggccacgggg acttgagcct gcaaattctt catttcctgc acaggaacgg
     2161 gaaggccgtt ttccccaacc gcgccggtgc ttagggcttt cggctcttcc ggattttgcg
     2221 gtccgtgcgt cagcggattt ttcccggccc ggcgaaggcg gatcttttcc gtgatggctt
     2281 ccgccgtcat ccggccgaac tggccgataa atcccgcaat cagaaccaga accacccact
     2341 tcagcgccga ccacgtgctg atgtaatcca gaatgtccgt cataaaggaa gcttcctttc
     2401 cggcggccgg cctttcacaa aaaaaccggc ggttccagcc tgcaatgctc ttatacagga
     2461 aaacacccgg ccatgacaag aactgttctg ctccggccaa aggttgaatt cattccgcca
     2521 tcatgataca gattcggcac attgatccat gaacaccgtc ggttcgggga gttttttcat
     2581 gcgcaaggcc atcgtgaagt cgattacgct gctgttgatc gcctgcgtct cggcttgcgc
     2641 gccgccgcct ctgcaaaaga aagatgataa gattgccgcg gccctcgcgg atccgcagac
     2701 ctcttttttt gaagcctgcc gaagaggaga catcgccatg gtcatgcgcc tggcggagga
     2761 aaaacggatc gacgtcaacc aagccgacga ttacgcctgg acaggcctga tgtacgccgt
     2821 tcaaaacgga cacaccggcc ttgccgaata ttttctcctt cagaaatcgc aaatcaaccg
     2881 gcaggatggg gacggactga cgccgctgat gatcgcggca aatgagcggc actcggacat
     2941 ggtggatctg ctgctggcgt tcggcgcgga cccgcttttg caggatcgac acggctatac
     3001 ggcgttgatg tgggccattt acaacaagag ctgggactgt atccccccct tgctggcaga
     3061 gggaaagggg ctcgatgtgc aaaacggctt cggggaaacg gcgctgcacc tggcgctcga
     3121 ctaccattgc tccgacattg ccggaatcct gatccggcaa aacgccaatg tgaagtttgc
     3181 ggacggcaga ggcagaactc ctttgcatct ggcggcggaa agaggcgatg aagacaatgc
     3241 ctggctcttg ctctccagag gagcggacgc aaatgccggg gatgaatccg gcgaaaatcc
     3301 tttgatgaaa agcattcaaa gcggcaatcc tgatctcgcc ttgctgcttc tccctcggac
     3361 cggaaacctt caggcaacca atcgggaggg tcgtacggcg ctgcacctgg ccgtcgtccg
     3421 ggatttgccg gacacggcgg ccgccctcat ccgccgcaaa gccgatctga acagctccga
     3481 cgagcagggc ttcacccctc tgatgcttgc cgcggagtcg ggttcggaaa gaatgacggt
     3541 tctgctggcg gagcgggggg ccgacctgaa gatgaagaac aaagagggca aaaccgcgct
     3601 tgatctggcc cgggaaaaaa gtcacggcaa catcgcaacc tatctggaaa gaaaaatggg
     3661 gaagccatga aggaacaacc ggaccttttc cgatggatgc ttgccgccgc gctcacgatt
     3721 gtccttatcg ccggcctgat aagttgccgg tatctcagca ttccggccca ggatgacgca
     3781 gtgcccgctc tccttccatc gaaggaagaa aaaggaccga ccgtttataa ttctgcggtg
     3841 atcaagggtc gcgtcaccgg acccgggtgg aagggacact ccacgctcat tctggcctgc
     3901 cgctccgcgg acaaaaaaaa cggatgcggc gaatacgcgg ccacccgtga aaatcaggat
     3961 gctttcatgc tgtatctgcc cgaggggcgc tattgtctta ttgccgtgac agaccacaat
     4021 cacgacggaa agtatgagaa ggacgaagct tccggcgcgt atggattacc tcgtcaaatg
     4081 atggaaatca ccgtccggga aggcgacctg atcaccggcg tggagatccg gaccgccgcg
     4141 gccggtgaac gcccggtgcg gctgccccgg gagtgccgct ggaaaatcga ggagcccgtc
     4201 attcggcagg caaccggcaa cggggatctt ctgaaaatct accatgaata tttttccctg
     4261 gaaaacgcgc agaccggcta ctggaatccg tcctccttca tgaaagcctt cggcgcgcat
     4321 atctacctga cgcaggccta cgattcacgg aaaatcccgc tcctcttcgt gcacggcacg
     4381 gagggaaccc cgcagaactg gatctattgt tacatgcgcc tggatcggga ccggtatcag
     4441 ccctggttct tttattatcc ttcgggaatc cgcctgaatc tggccgcggc gctgcttcag
     4501 gaagaactgc gggagcttca ggaacaatac ggctttcgaa aaatggccct cgccgcgcac
     4561 agcgtaggcg gactgaccgc aaggtctttt ctgattcgcc gcgaatcgga agaaaggcct
     4621 cccttcatcc ggctgtttgc atccttcgcc acgccctgga acggattcgg cctggccgac
     4681 gcctcccggg tgctgccgca caaaagcatt ccggtgtggc tggatctggg aacgcacagc
     4741 gaatttattt ccgccaccat gaacgccaaa ttgcctccct cggtccgcca ctatcttttt
     4801 tacggcaggg aagacaaact cacgggaggc gcggagccgg acagccgggc aacagccggc
     4861 acggccggcg tgtatggatt cgactgcacg cacgacggca tcctgatcga ccgcagggta
     4921 ttttcccgat tcaacaccat cctggaaaag gagatgaact gaaatgaaaa tcatccggtt
     4981 tctttccgaa agcggaaaga cgctttacgg cgcgtacagc aagcgccggc cggggacggc
     5041 cgaaggaatc gaaaaagaac cctggagcaa gaacatcaca ggcggcggtg aagaaaaaat
     5101 ccggaaactg ctggcgcccg tcgcgcccgt caatattctc gcgctgggca ttaattaccg
     5161 aaagcacggc gaggaaaccg ccctgtccat cccttcggag ccgatcctgt ttctcaaagc
     5221 caccagcagc gtcaccggac ccgatcatcc cattgtgttg cccgccgccg ggtccgatca
     5281 ggtggactat gaagcggagc tggccctggt catcggaaag acggccaaaa atgtcacgcc
     5341 tgcggaagcg tgggattgca ttttcggctt cacctgcgcc aatgatgtga gcgcgcgcga
     5401 ctggcagttc gaaaggcaga aaggccagtg ggcccgggga aaaagctttg acaccttctg
     5461 tccgctggg
//
LOCUS       DAOSIM010000135         5439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_13473_length_5439_cov_12.335810, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000135 DAOSIM010000000
VERSION     DAOSIM010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13473_length_5439_cov_12.335810"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PLR47_06765"
     CDS             complement(<1..709)
                     /locus_tag="PLR47_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOG12684.1"
                     /translation="MPIRYYSTNRRVQQASGITPFAESVSFREALLAGQAPDEGLFVP
                     DAIPQLSMKDIIALRDRPYWEAALLVARAFLSDEFSPEVLDAIVKDAYNFPVPLEAVY
                     PRAFILRLDQGPTASFKDFAARMMARLMSHCRPEGERLNILVATSGDTGSAVGEAYKG
                     VDGIDVTILYPADEVSLMQKKQLDTIGGNVRAMCVDGKFDDCQNLVKEAFADKDLARF
                     NLSSANSINFGRILPQIV"
     gene            912..1964
                     /gene="selD"
                     /locus_tag="PLR47_06770"
     CDS             912..1964
                     /gene="selD"
                     /locus_tag="PLR47_06770"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:963..965,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOG12685.1"
                     /translation="MEEKKTVRLTETVQSAGUACKIGPGDLSDILSDLPLISDPKLVT
                     GYEHAEDAGVYKITDDLALVQTVDFFTPTVDDPFTFGQIAAVNALNDIYAMGGTPLTA
                     MNIVCFPIKTMDKSVLREVLRGGLDKMREAGVLLVGGHSVEDNEIKYGLSVTGTIHPD
                     RVLFNRGARPGDRLILTKPLGTGIVATAIKAGAAGQALVEQATRCMTTLNKKAAELMI
                     AAGDIHSCTDITGFGFLGHACEMIEGSDVGLRIEASRIPILEGVLDLTQTGYLPGGLY
                     RNRNYRLHQVEKAPSCPDWIFDVCFDPQTAGGLLFSLPSEKAEPLAAAMRRAGITDAV
                     VVGDVVSDDPGKILME"
     gene            1971..3035
                     /locus_tag="PLR47_06775"
     CDS             1971..3035
                     /locus_tag="PLR47_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12686.1"
                     /translation="MNRLPFKSETERNVRKLLELAHSDDSLLLLMYGSPDPDAIASAM
                     ALREILLRTKGLSKWVFAATEPIRRQQNRELAQAMRLNIRLLNQVDAGAYRLVALVDA
                     QPSFFGDSLPAREPNIVLDHHPREGNWQAELADIRPLYGALSTILTEYLLVARVRIPR
                     NLHTALLYGIKTDTDNFDRETIAEDIRAYTYHSRHANLRLIRRIELNQTPHSYLKYFD
                     YAFHSMTHFRGRRVGFLGRVESPDVCVQVADFYLHVIGTSYVVVAGIVDDRLVIIFRG
                     DGYRQDCGAMAQQACGQIGQGGGHRSAARLEIPLDVLKTELKEDLSRQSVERFLHQCL
                     RKSHLPKEDPPAAGSGREKT"
     gene            3032..4069
                     /locus_tag="PLR47_06780"
     CDS             3032..4069
                     /locus_tag="PLR47_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12687.1"
                     /translation="MKDIGFHRETVTGRYLNPFDGFEPREMTIEERYDETTGVASRIL
                     PYRVRPAQKPDTGVYLEKSPESICPFCPELFDKMTPRFTPDIIEGGKFTRGSAFLFPN
                     AFPYDATNTVAIFSHRHFMGLDELTPDAMHDGFAVCRDYFYRMAELQQGYHYGSINWN
                     YMPPAGGGLIHPHLQTIMGKEPTSFMRRLLASARNYASASGGRNLWEDLLDREQEAGE
                     RFIASTGVVSWLAAFSPKGMAGEVDFYFREKTSFFDLTQANVEELLEGLSRIFLHLYV
                     NHFMSFNLSLYAPLMPEKNFWVQGKIVPRFEINPLGTSDINYFEKLHDEIICPIVPEQ
                     LCKELQGYFQT"
     gene            complement(4163..4447)
                     /locus_tag="PLR47_06785"
     CDS             complement(4163..4447)
                     /locus_tag="PLR47_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOG12688.1"
                     /translation="MPIYEYQCRKCGRQFEAFQGITEPDLKTCRFCRGKVQKMMSLSS
                     FSLKGTGWYATDYKGKKPQAAKPKSTETTATESSSASSDASATVKGTSDD"
     gene            complement(4465..5001)
                     /locus_tag="PLR47_06790"
     CDS             complement(4465..5001)
                     /locus_tag="PLR47_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12689.1"
                     /translation="MIRYFNAAHIPVAARAFSDAEKLVTRHFRLTEDDLRKNKYDVKT
                     LAFLEEHEVNDGAFAHLCKYSYEKSSDGKPQEKEGFEFYRVCLQDHIILDALERANTF
                     IKLSPLMLYIAVHELIHVLRFSSGAADFSAPADEKENEEKIVHSLTRSALQPARQTDM
                     NMVLDCFSSEYKISNLYN"
     gene            5184..>5439
                     /locus_tag="PLR47_06795"
     CDS             5184..>5439
                     /locus_tag="PLR47_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12690.1"
                     /translation="MAQTGNCRNKNENDENTVWGMAVLRPFALIGRNVLSWVDNLGAA
                     AIFLGMALWQIFRRKQFAKTVRQVYYIGARSITIIMLVSLF"
BASE COUNT         1173 a         1493 c         1537 g         1236 t
ORIGIN      
        1 aaacgatctg cggcagaatg cgcccgaagt tgatcgagtt ggccgacgac agattaaacc
       61 ttgccaggtc tttgtcggca aacgcttctt tgaccagatt ctggcagtcg tcgaactttc
      121 cgtcaacgca catggcccgc acgttgccgc cgatggtgtc gagctgtttt ttctgcatca
      181 ggctcacttc gtccgccggg tacaggatgg ttacgtcgat gccgtcaacg cccttgtagg
      241 cttctccgac ggcgcttccc gtgtcgccgg acgtcgccac cagaatgttg agcctttccc
      301 cttcaggccg gcagtggctc atgagccggg ccatcattcg ggcggcgaaa tccttgaagg
      361 acgccgtggg gccctggtcc agccgaagaa tgaaggcgcg gggatacacc gcttccagcg
      421 ggacggggaa attatacgca tccttcacga ttgcatcgag gacttcgggt gaaaactcat
      481 cggagagaaa cgcccgggcg accagcagcg ccgcctccca atagggccgg tcgcgaagcg
      541 caataatatc cttcatggaa agctgcggaa tggcgtcggg aacaaacagg ccctcatccg
      601 gcgcctgtcc ggccagcaac gcttcccgga aagatacgct ttcggcaaag ggcgtaatgc
      661 cggaagcctg ttgaacgcgg cgattggtgc tgtagtagcg aatgggcatg cccggtatcc
      721 tttgtgaact ggcttcctat aatctgaatg acgccaataa atcaacaaaa aacaatcctc
      781 tctgtccgcc gtccttcaat gccccgccgg ggcggacgtg aaaagcgtct gaattccgtt
      841 tggaaaatcg gcgcttctat gttaaaaaac caaacgccgt gaggcgcaaa ttctttttgg
      901 attggaacat catggaagaa aaaaagaccg ttcgtctgac ggaaaccgtc caaagcgcgg
      961 gttgagcctg caagatcggt ccgggggacc tgtccgacat tctcagcgat ttgccgctca
     1021 tctccgatcc gaaactggtg accgggtatg agcatgcgga agacgcgggc gtgtataaaa
     1081 tcaccgacga cctggcgctg gtgcagaccg tggatttttt caccccgacc gtggatgatc
     1141 ccttcacctt cggtcaaatc gccgccgtca atgccttaaa cgacatttac gcgatggggg
     1201 gcactccgct caccgccatg aacatcgttt gttttcccat taagacgatg gacaaatcgg
     1261 tgctgcgcga ggttttgcgc ggagggctgg acaagatgcg cgaagccggc gttctcttgg
     1321 tcggtggcca cagcgtggaa gacaacgaaa tcaagtatgg tctgtcggtg accggcacga
     1381 ttcatccgga ccgcgtgctg ttcaaccgcg gcgcccgccc cggtgaccgg ctcattctga
     1441 ccaagccgct gggtacgggc attgtcgcga cggccatcaa ggcgggggcg gccgggcagg
     1501 cgcttgtcga acaggcaacg cgctgcatga cgacgctcaa taaaaaagcg gccgagctga
     1561 tgatcgcggc gggagacatt cattcctgca cggatatcac gggcttcggt tttctcggac
     1621 acgcctgtga aatgattgaa ggcagcgatg tcggcctgcg gatcgaggcg tccagaattc
     1681 cgattctgga gggcgtgctg gatctcactc aaaccggcta ccttcccggc ggtctttacc
     1741 gcaaccggaa ctaccgcctt catcaggtcg agaaagctcc gtcttgtccg gactggattt
     1801 ttgacgtctg ctttgatccg cagacggcgg gaggtctgtt gttcagcctg ccttccgaga
     1861 aggcggagcc tcttgcggct gccatgcgcc gggcgggcat cacggacgcg gtcgtcgtgg
     1921 gcgatgtggt cagtgatgac ccgggtaaaa ttctcatgga gtaaagtcct ttgaatcgct
     1981 tgcccttcaa aagtgagact gagcggaatg tcagaaagct cctcgagctc gcgcattcgg
     2041 acgattcgct gctgttgctg atgtacggca gcccggatcc ggacgcaatc gcttcggcca
     2101 tggcgcttcg cgaaatcctc cttcggacca aaggtctttc caaatgggtt tttgcggcga
     2161 cggagccgat ccgccgtcag cagaaccggg aactggcgca ggcgatgcgc ctcaacattc
     2221 ggcttctgaa tcaggttgat gccggcgcct accggctagt tgcgctggtg gacgcacagc
     2281 cttctttttt tggagattcc ctcccggccc gcgagccgaa tatcgtcctg gatcatcatc
     2341 cccgcgaggg aaactggcag gccgaacttg ccgacattcg gcccttgtac ggcgcgctct
     2401 cgacgatttt aacggaatac ctgctggtcg cgcgggtgcg cattccccgc aatctgcaca
     2461 cggcgctgct ttacggcatc aagacggaca cggataattt cgaccgcgaa acgattgccg
     2521 aagacattcg cgcctacacc tatcattccc ggcacgccaa cctgcggctg atccgccgga
     2581 tcgaactcaa tcagacgccg cacagctatt tgaaatattt cgattacgcg tttcattcca
     2641 tgacgcattt ccgcggccgc cgcgtcggtt tcctgggacg cgtggaaagt ccggatgtct
     2701 gcgtgcaggt ggcggatttc tacctgcacg tgatcggcac gtcgtacgtg gtggttgcgg
     2761 gaatcgtgga tgaccggctg gtcattattt tccgggggga cggctaccgg caggactgcg
     2821 gcgcgatggc gcagcaagcc tgcggccaga tcggacaggg aggcggacac cgcagcgccg
     2881 cgcgcctgga aatccccctg gacgttctca aaacggagct gaaagaggat ttgtcccgac
     2941 agagcgtgga gcgttttctt catcaatgcc tgaggaaaag ccatttgccg aaagaagatc
     3001 cgcctgcggc cggaagtggg agggaaaaaa catgaaagac atcggatttc acagggaaac
     3061 ggtcaccgga cggtacctca atccctttga cgggtttgaa cccagggaaa tgaccattga
     3121 ggagcgctac gacgaaacca ccggcgtggc cagccgcatt cttccttacc gtgtgcggcc
     3181 cgcccagaaa ccggacacag gcgtctatct ggaaaaatcg ccggaatcga tctgcccgtt
     3241 ttgcccggag ctctttgaca agatgacgcc ccggttcacg ccggacatca tcgaaggcgg
     3301 caaattcacg agaggaagcg cttttctgtt tcccaacgcg tttccctacg acgcgacaaa
     3361 cacggttgcg atcttttcgc accgacactt catggggctg gatgaattga cccccgacgc
     3421 catgcacgac ggatttgccg tctgccggga ttatttttac cggatggccg aactgcagca
     3481 agggtaccat tacggttcga tcaactggaa ctacatgccg cccgcgggcg gcggactgat
     3541 tcatccccac ctgcagacga tcatgggcaa agagccgacc agtttcatgc ggcggcttct
     3601 ggccagcgcg cggaattacg cctccgcctc cggcggccgc aatctgtggg aagacctgct
     3661 ggatcgggag caggaagcag gcgagcgttt tattgcctcg accggcgtcg tgtcttggct
     3721 tgccgctttt tcgccaaagg gcatggcggg ggaagttgat ttttactttc gggagaaaac
     3781 ctcgtttttt gatctgaccc aggccaacgt tgaagagctt ctcgaaggtc tttcccggat
     3841 ctttctccac ctgtatgtca atcatttcat gagcttcaat ctttcgctgt atgcgcccct
     3901 gatgccggag aaaaacttct gggtgcaggg gaaaatcgtt ccgcgttttg aaatcaatcc
     3961 gctggggacg agcgacatca attattttga aaaactgcac gatgagatca tctgtccgat
     4021 tgtgccggag cagttgtgca aagaattaca ggggtatttc cagacataga ggagggaacg
     4081 gtttcaaacc gttcttcgca aaaaaaacaa aaaagcggca ttttcatgcc gctttttgtt
     4141 ggcctttttt aagtgaatgc ttttaatcgt cgcttgtgcc tttgacggtt gccgaagcat
     4201 cggatgaagc cgaagaactt tccgtcgcag tcgtctcagt gcttttcggc ttggcggcct
     4261 gaggtttttt tcccttgtag tccgtcgcgt accagccggt gcctttcagg ctgaaggatg
     4321 aaagcgacat cattttctga accttccccc tgcagaaccg gcaggttttc aaatccggct
     4381 cggtaatgcc ctgaaacgct tcaaactgcc ggccgcattt cctgcattga tattcataga
     4441 tcggcataca tcaacctcct gaaattaatt ataaagattg ctgattttat attcgctgct
     4501 gaagcagtcc aaaaccatgt tcatatccgt ttgcctggcc ggctgcagcg cgcttcgggt
     4561 caggctgtgg acgatttttt cctcattttc cttttcgtcc gcgggggcgc tgaaatcagc
     4621 ggcgcccgag gagaagcgca ggacgtggat caattcatgg acggcaatat acagcatcaa
     4681 aggactcaat ttgatgaagg tattggcgcg ctccagtgcg tccaggatga tatggtcctg
     4741 caggcacacc cggtaaaact cgaaaccttc cttctcctgc ggcttgccgt cggaagactt
     4801 ttcgtaggaa tatttgcaca ggtgtgcaaa tgcgccgtca tttacttcat gctcttccaa
     4861 aaatgcaagg gtttttacat cgtatttgtt tttccgcagg tcatcttccg tcaggcggaa
     4921 atgacgcgtc accagctttt cagcgtcgga aaacgccctg gccgccaccg gtatatgcgc
     4981 cgcgttaaaa tagcgaatca taaaaacacc ttaaatgccg ccgcccgatt tggccgtagt
     5041 atattcattc ttcgtgtttc tgtcaacacg gacgggaaca cccggacggg aaaccggcgt
     5101 caggaaatgc ttgtcaaata aagttgcctc tgttagtatc ccgaaccgtc ttcgacaggc
     5161 gatttgatct ggggaacttg ttcatggcgc aaacgggaaa ttgccggaat aaaaatgaaa
     5221 atgacgaaaa caccgtctgg ggcatggctg ttcttcgccc gtttgctttg atcggaagaa
     5281 acgttttgag ctgggtggac aacctcgggg cggcggccat ttttctgggg atggcgctgt
     5341 ggcagatttt ccgcaggaag cagtttgcca agacggtccg gcaggtttat tatatcgggg
     5401 ccagatccat cacgatcatc atgctggtca gcctgttta
//
LOCUS       DAOSIM010000136         5435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_13484_length_5435_cov_6.436803, whole genome shotgun sequence.
ACCESSION   DAOSIM010000136 DAOSIM010000000
VERSION     DAOSIM010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13484_length_5435_cov_6.436803"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..790)
                     /locus_tag="PLR47_06800"
     CDS             complement(26..790)
                     /locus_tag="PLR47_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOG12691.1"
                     /translation="MIIFDVFAFIFGAAIGSFLNVCIFRIPAEASIVKPASRCPSCGH
                     PIRFHDNIPIVSFLILRARCRDCGAKISWRYPLVEMITGLLALLLFLKYGPTLVFLSF
                     FIFTAVLIVITFIDLDHQIIPDILSLPGIPVFFLAAVFIVGLPWLEALIGLLIGGGVL
                     LAIAFVYECITKREGMGGGDIKLLAMIGGFLGWKSLIFVLLFSSLTGAVVGIAAMVVK
                     KQDTKYAIPFGPFLSAAAVAWIFWGDAFMRFLIFRN"
     gene            complement(943..1272)
                     /locus_tag="PLR47_06805"
     CDS             complement(943..1272)
                     /locus_tag="PLR47_06805"
                     /inference="COORDINATES: protein motif:HMM:NF017355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HOG12692.1"
                     /translation="MPLKNDRYTYRVTWSEEDQEYAGLCTEFPSLSWMDKTPEEALKG
                     IRKIVQGVIEDMKKTGEEIPEPIVSKKFSGKFMVRVPPEVHRNLAIESSESGISLNRL
                     VGAKLSR"
     gene            complement(1256..1516)
                     /locus_tag="PLR47_06810"
     CDS             complement(1256..1516)
                     /locus_tag="PLR47_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12693.1"
                     /translation="MAKVEDIIAQMRNNPANVRFADLSNVCNKYFGAPRHQDGSHHIY
                     KTPWKGNPRINIQNQKGKAKAYQVKQVLLAIELMEVEHATEE"
     gene            1623..2705
                     /gene="ribD"
                     /locus_tag="PLR47_06815"
     CDS             1623..2705
                     /gene="ribD"
                     /locus_tag="PLR47_06815"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOG12694.1"
                     /translation="MNDEEYMKLAIKLAAKGRCYASPNPMVGAVIVKNGQIIGRGYHQ
                     CCGQNHAEVNAIADAKGKVAGSTLYVTLEPCCHSGKTPPCTDLIIRHKIRRVVIAAAD
                     SNPLVSCRGIACLENAGIEVTRGVLEAESRSLNEVFFHFMETGLPFITVKYAQTLDGR
                     IATATGNSQWISSPASLKYAHRLRAEHDAILVGIGTVLRDNPTLTVRLVRGRNPLRVI
                     VDSNLATPADANVMRNLSETPTLIATTQKPSDPRYRKLSEAGARLMTVAPDREKHVDL
                     KKLFQKLARENTSSVLIEGGAAVITSALGQGLVNRLVVVTAPKILGKGIEAVGDLNIR
                     ELTDAKILSVRVLSKKGPDVIMDARL"
     gene            complement(2686..4377)
                     /locus_tag="PLR47_06820"
     CDS             complement(2686..4377)
                     /locus_tag="PLR47_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12695.1"
                     /translation="MNEIRLNGLTLALDEKEESLKEKTARALGTAAADIASCRVVKKA
                     LDARRSKPPRFVYALHVSLQPNVILPDVLPEGLQIQKWEGGPDGCSVVRETKETTSCV
                     SAQPESASLLHDGGQFVKASKGEMQPVVVVGSGPAGLFAAYRLSLAAIPVILLERGRP
                     IEQRAADVRLFWEQGILHPESNVLFGEGGAGTFSDGKLTSRSKNPAAGWVKKVLVEMG
                     APKAILTDAKPHIGTDRLRLVILRLRQKLIELGCDIRFENRVTDIVIRDGKIAAVAIN
                     DRQEIETDCVILAIGQSADDTYRLLQERGVSMEPKPFAMGLRVEHPQDLINSIQYGRW
                     AGHPQLPPAEYFVTAAVEEQNRSVYTFCMCPGGQVIGCSAFPGRVITNGMSNAARSGA
                     FANSAVVVNVRTDDFARAEGPLSGLAFRDEWERKAFEAGGGRYEAPAQKLVEFMEGKF
                     SGSVGQTSFMPGAKAARLSDVLPDFVAGALREGLRRINQKMPGFISPAAHLIGVETRT
                     SAPVRMRRGRDGQSENVRGLYPCGEGSGYAGGIISSALDGLKAAQSAAAAIIGRP"
     gene            4530..5030
                     /locus_tag="PLR47_06825"
     CDS             4530..5030
                     /locus_tag="PLR47_06825"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HOG12696.1"
                     /translation="MPWYAVSTKSRHEYKAYTGLCQKSLTTFLPEMEVWSKRKDRKKK
                     ITVPLFPGYLFVNAVLDNETKLTILKTIGVVRILGKKLNTEPLPVPEEKILAIQRILE
                     KKVELFSLQYPKAGEPARIMDGPFAGIEGTVITSDPKKELFVITIDLMQRSVAIKLEG
                     FQISKI"
     gene            5221..5349
                     /locus_tag="PLR47_06830"
     CDS             5221..5349
                     /locus_tag="PLR47_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12697.1"
                     /translation="MPSKEYNVQGYHPVCADETKECNGCAICATMCPEIAIEVYRE"
BASE COUNT         1326 a         1512 c         1386 g         1211 t
ORIGIN      
        1 gcatgggcgg cgcttttcac cggttttaat tcctgaagat caaaaacctc atgaaagcgt
       61 ctccccagaa aatccacgcg acggcggccg ccgacagaaa aggcccgaag ggaatggcgt
      121 atttggtgtc ctgctttttg acgaccatgg ccgcaattcc cacaaccgcg cctgtcagag
      181 aactgaacag caaaacgaag atcagtgatt tccaccccag aaaaccgccg atcatcgcca
      241 gcagcttgat gtcgccgccg cccatgcctt cccgtttggt gatgcattca taaacgaagg
      301 caatggccag aaggacgccg cctccgatga gcaatccgat caaagcctcc agccagggca
      361 ggccgacgat aaaaacggcg gccagaaaaa agaccgggat gccgggcaga gacaaaatat
      421 cgggaatgat ctgatgatcc agatcgatga aggtgatgac gatgagaacg gctgtaaaaa
      481 tgaaaaaaga tagaaaaaca agggttggac cgtatttgag aaaaagcagc aacgccagca
      541 ggcctgtgat catttcgacc agcggatagc gccaggagat tttggccccg cagtctcggc
      601 atctggcgcg caaaatcagg aagctgacaa tcggaatgtt gtcgtgaaaa cgaatcggat
      661 gtccgcagga aggacagcgg gaagcaggct tcacaatgga ggcttccgcc ggaatgcgga
      721 aaatgcagac gttaagaaag ctgccgatcg ccgcgccgaa gatgaaggca aaaacatcaa
      781 aaataatcat aaataatacc ttttgccgtc attgcgggct tgatccggaa tccagtattt
      841 tctataattt tctggatacc ggcctacgcc ggtatgacga cttttattct gtttcccgcc
      901 attgcgacac tgcctgatcg cggaatgaca gcttgcgtga ttttatcggc ttaacttcgc
      961 gccgacaaga cggttcaggc tgatgcctga ttccgatgac tcaatggcca gatttcgatg
     1021 aacctcaggg ggaacccgca ccataaattt accgctgaat ttcttactaa cgatgggttc
     1081 gggtatttct tcacccgttt ttttcatatc ctcgataaca ccctggacaa tcttgcgaat
     1141 acctttcaac gcctcttccg gtgtcttatc catccagctc aggctgggaa attcagtgca
     1201 caaaccagca tattcctgat cctcttccga ccaggttacc cgataggtat aacgatcatt
     1261 cttcagtggc atgttcaacc tccattagct caatggccaa aagcacctgc tttacctgat
     1321 atgctttagc ctttcctttc tgattttgaa tatttatgcg cggattgcct ttccatggcg
     1381 ttttatatat atggtgactt ccatcctgat gtcgcggcgc gccaaaatat ttattgcaaa
     1441 cattgctcaa atcggcaaac cgaacattcg cgggattgtt tctcatctgc gcaatgatat
     1501 cttctacttt agccatgctg aagatagtat caatattgat acttatgtca agacatttct
     1561 aacacataca tgccatcaga atttctttgc tttgattgtt tcattgaggt aatggcattt
     1621 tcatgaacga cgaagaatac atgaagctgg cgatcaaact ggccgcgaaa ggccggtgct
     1681 atgcaagccc caatccgatg gtgggcgccg tgatcgtcaa aaacggccaa atcatcggtc
     1741 ggggatacca tcagtgctgc gggcaaaatc acgcggaagt gaacgcgata gcggacgcga
     1801 agggaaaggt tgccggatcg acgctttatg tgacgctgga gccctgctgc cattccggca
     1861 agacgccccc ctgtaccgac ctcatcatcc gccacaaaat caggcgcgtc gtgattgccg
     1921 ccgccgactc caatcccctg gtttcctgcc ggggcatcgc ctgcctggaa aacgcgggaa
     1981 tcgaagtcac ccggggcgtg ctggaggctg aaagccgaag tctcaatgaa gtcttctttc
     2041 attttatgga aacggggctg ccctttatca cggtcaaata cgcccagacg ctggacgggc
     2101 gcatcgccac cgccacgggg aactcccagt ggatcagctc tcccgcttcc ctgaaatacg
     2161 cccaccgcct gcgcgcggag cacgacgcca ttttagttgg aatcggaacc gtgcttcggg
     2221 acaacccgac gctgacggtc cgtctggtcc ggggtcgcaa ccccctgagg gtgatcgtgg
     2281 attccaatct ggccacgccg gctgatgcga atgtgatgcg caatctgtcc gaaaccccta
     2341 ccctgatcgc gacgacccaa aaaccttccg atccccgcta cagaaagctt tcagaagccg
     2401 gcgcccgtct gatgacggtt gcgcccgacc gggaaaaaca tgttgatttg aagaaattgt
     2461 ttcaaaaact ggcccgcgaa aacacatcat cggtactgat tgagggtggc gccgcggtca
     2521 tcacctccgc gctggggcag ggcctggtca atcgactggt cgttgttaca gcgcccaaga
     2581 ttctcggcaa aggcattgaa gccgtaggcg atctgaacat tcgggagctc acagacgcga
     2641 aaatcctgtc cgttcgcgtt ctttcgaaaa aaggcccgga tgtgatcatg gacgcccgat
     2701 tatagccgcg gccgcgcttt gggcggcctt gaggccgtcg agcgccgaac tgataatccc
     2761 tcccgcgtat cccgatcctt ccccgcaggg ataaagtccc cgaacgtttt cactctgacc
     2821 gtccctgcct cgtcgcatgc gcacgggcgc cgaggttcgg gtttccacgc cgatcagatg
     2881 cgccgcaggc gaaataaagc ccggcatttt ctggttgatc cgccgaagcc cttcccgcag
     2941 tgcgcctgcg acaaaatcgg gaagcacatc ggagaggcgc gcggccttgg cgccgggcat
     3001 gaaactggtt tggcccaccg aaccggaaaa tttcccttcc ataaattcga caagcttttg
     3061 cgccggcgct tcgtaacggc cgccgcccgc ctcaaaggcc ttccgctccc attcatcacg
     3121 aaaagcaagg ccggaaagcg ggccttccgc ccgggcaaaa tcgtccgtgc gcacgttgac
     3181 gacgaccgcg ctgttggcaa aggcgccgct tcgggcggcg ttgctcatgc cgttggtgat
     3241 cacgcggccc ggaaacgcgc tgcagccgat cacctgccct cccggacaca tgcaaaacgt
     3301 gtaaacggag cggttttgtt cttccacggc ggcggtcaca aaatattccg ccggcggcag
     3361 ttggggatga ccggcccacc tgccgtattg aatagagttg atcaggtcct gcggatgttc
     3421 gacgcgcagc cccatcgcga aaggcttcgg ctccatgctg acgccccgct cttgcaaaag
     3481 acggtaggtg tcgtccgcgc tctgtccgat ggcgagaatc acacaatccg tttcgatttc
     3541 ctgccggtcg ttgattgcca ccgccgcgat tttgccgtcg cggatcacaa tatccgtcac
     3601 ccggttttca aagcgaatgt cgcaccccag ctctataagt ttttgcctca agcgcagaat
     3661 cacaagacgg agccgatccg ttccgatatg cggcttggcg tccgttagaa ttgccttcgg
     3721 agcacccatt tccaccagca cttttttgac ccatcccgcg gcgggatttt tggaccggct
     3781 ggtcagtttt ccgtcggaaa aagttcccgc gccgccctcg ccgaacaaaa cattgctttc
     3841 cggatggaga atgccctgct cccagaaaag cctcacgtcc gccgccctct gttcgatggg
     3901 ccgtcctctt tccagaagga ttacgggaat ggccgcgagg gaaagcctgt aggcggcaaa
     3961 aagccccgcc ggcccgctgc cgaccacgac cacgggttgc atttcccctt tcgacgcttt
     4021 tacgaactgg ccgccgtcgt gaagcagcga tgcactttcc ggctgtgctg aaacgcagga
     4081 tgttgtctcc tttgtttcac ggaccacgga acaaccatcc ggtccccctt cccacttctg
     4141 aatctgaaga ccttccggca gaacgtcagg caagatgacg tttggctgga gagaaacatg
     4201 gagcgcatac acaaaacgag gaggcttgct gcgcctggca tccagagctt ttttgacaac
     4261 acggcaggaa gcaatgtcgg cagccgccgt acccagcgcg cgggccgtct tttccttcag
     4321 gctttcctct ttttcatcca gcgcaagcgt cagaccgttg aggcgaatct cattcatcat
     4381 ttctccattg caaaaaacaa acacatgcta tagtcattca cggttctgaa gacccgtgct
     4441 acattgtttg tgtagtgcgt acctttaggt gcgcttgaaa gctttgccat gattctgttt
     4501 tcacagtaat gactttaaag gggtttgcaa tgccctggta tgccgtttcc acaaaaagcc
     4561 gtcatgaata caaagcctat accggacttt gccagaaaag cctgacaacg tttttaccgg
     4621 agatggaagt ctggagcaag cgaaaagacc gcaaaaagaa gatcacggtg ccgctttttc
     4681 ccggttatct tttcgtcaac gccgttttgg acaatgaaac caaactgacg attctcaaaa
     4741 ccatcggcgt cgtccggatt ctgggaaaga aattaaacac ggaacctttg ccggtgccgg
     4801 aagaaaaaat tcttgccatc cagcgtattt tggaaaagaa agtggagctg ttctccctgc
     4861 aatatccgaa ggcgggagag ccggcgcgga ttatggacgg accctttgcc ggcattgaag
     4921 gcacggtaat aacaagcgat ccgaaaaaag aactgttcgt catcaccatc gatttgatgc
     4981 agcgctcagt ggccattaaa ctggaaggtt ttcaaatcag caagatctga aaaccttaaa
     5041 tttcagccta aaatcccttg actttctgaa cattatgagc aagatagcat ccgccgcaag
     5101 gaaacaatta acaggtaaaa actgatctgg ggacatgttg tgaaaggata tatcaagatt
     5161 aagaaggaac tttgcaagga gtgtcaactc tgtattcatt tctgccccaa aggccacatc
     5221 atgccgtcca aagaatacaa tgttcaaggg tatcatcccg tgtgcgccga cgaaacgaaa
     5281 gaatgcaacg gctgcgcgat ttgcgccacc atgtgtccgg aaatagccat tgaggtttat
     5341 cgtgaataag gtattgatgt caggaaataa tgtgatcggc gaagccgcga ttcgcgcggg
     5401 atgccgattt tatgccggtt atcccattac gccgc
//
LOCUS       DAOSIM010000137         5383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_13624_length_5383_cov_10.011449, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000137 DAOSIM010000000
VERSION     DAOSIM010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13624_length_5383_cov_10.011449"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="PLR47_06835"
     CDS             <1..1042
                     /locus_tag="PLR47_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG12698.1"
                     /translation="AISSPLMDFLGGLGIAAVVFYGGYNVVQGHSTPGTFFSFIAAML
                     MLYEPIKRLTNVNNTINQGIAGADRIFSIIDRTPDIEDRPGAVTLPPVSRGIDIENVT
                     FCYETTPVLKNINLSIKAGEVIAFVGMSGGGKTSLVNLIPRFYDVTEGRILIDGHDIR
                     NVSLQSLRGQIAIVTQQTILFNDTVRNNIAYGDIRKTDEDIYRAARAANAHDFILRLP
                     DGYDSNIGELGTKLSGGEKQRISIARALLKDAPILILDEATSSLDTEAEIEVQEALDN
                     LMKGRTTLVIAHRLSTIRNADRIIALVNGEIVEEGNHDALMEKKGEYYRLYNLQFKDE
                     GNGNDRLARDME"
     gene            1014..2117
                     /gene="lpxK"
                     /locus_tag="PLR47_06840"
     CDS             1014..2117
                     /gene="lpxK"
                     /locus_tag="PLR47_06840"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOG12699.1"
                     /translation="MIDWPGIWNENDSSRRISPTRAILYLGSLVYRLIVSSRNRLYDR
                     QILKFVRLARPVISVGNLTVGGTGKTPCVIGLAKMLARRGYRPVVLSRGYGGKNQASV
                     NIVSDGKNIMLGAEEAGDEPLLIARSLPGIPVLTGAKRALTGQAAIERFNADVLICDD
                     AFQHRQIFRDIDIVLLDADRPLGNDRLLPRGELREPAGSLRRADCIVLTRADKAGTPP
                     DEIARITRERSIPVFRSVHRLREMVRPREHLTLPPENFRGKSICAFCGIARPASFKKS
                     LLDAGMHILSFIDFPDHYAYKRDDLEAIQKHFSTQNADAWITTEKDAMRLEESPDFLN
                     QLFIARMEMEIQPSTPSFDDFILRRLKDLILKT"
     gene            2133..3020
                     /locus_tag="PLR47_06845"
     CDS             2133..3020
                     /locus_tag="PLR47_06845"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG12700.1"
                     /translation="MSLKLKIGLWIFRVIPLAVRRAVFSGLAWLGYRLSVKHRLIVLH
                     NLTRAFPEKSPAEIVRIAKASYRSFGRVVAEFSEITRLNPDNVHQWVRIQGLEHYDEA
                     RRKGKGVLLFSAHFGNWEIGNAAMAIARKPLIFIYRILDSQFLEEAITYVRATCGNIS
                     LDKENAMRPMIRALKKGETINILIDQNVAVYDGIFVDFFGRPACTTSGLALLALHTGA
                     PVLPVFTTRMPDGKYWLEIGAEVAIRNTGNRDADVRESTQTFTGIIEEHIRKYPEQWF
                     WMHQRWKTKKCQARMVNSE"
     gene            3031..4116
                     /gene="waaF"
                     /locus_tag="PLR47_06850"
     CDS             3031..4116
                     /gene="waaF"
                     /locus_tag="PLR47_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOG12701.1"
                     /translation="MMMLEAKNKLPREGLNKILIRGTNWIGDAVMTLPAVASVRAAYP
                     KAHLSILAKPPVSDIYKMFSAADEILAYSSKFDNAPGVLRLAYSLRSKKFDAAILLQN
                     AIEAAIIALAAGIRVRAGYNSDGRGFLLTHPVRRTTEILRVHQIGYYLEMVKALGCAN
                     VERSPHLETFISAADATDVVRRHLTDMAGPFIGIAPGAAYGPAKRWLPERFAQAGDRL
                     AKELNAQVLLFGGRDDWETTEQVREQAQTNMINLAGRVPLADSVYLMSQCRLFLSNDS
                     GLMHVAGGLNIPTVAIFGSTNPVTTSPAGEKTILVRKETPCSPCLEKTCPTDFRCMTA
                     ITVEDVVAAALTLLNGPANPRRNDQKS"
     gene            4123..4710
                     /locus_tag="PLR47_06855"
     CDS             4123..4710
                     /locus_tag="PLR47_06855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG12702.1"
                     /translation="MKKEAAIFLDRDGTINEEVGYLDSLDKLAIFPSAFEAIRLINQS
                     GLKTIVVTNQAGVARDFFGEDFVETIHAALREALCQRGALIDAFYYCPHHPTEGFPPY
                     RQSCDCRKPAPGLFLRAAMDWNLDLSASWMIGDRYNDIEAAHRAGARGVLVKTGYGAD
                     ALAGAGPDGETLAGKPDLIADDILQAVHLILGSRK"
     gene            4707..>5383
                     /locus_tag="PLR47_06860"
     CDS             4707..>5383
                     /locus_tag="PLR47_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOG12703.1"
                     /translation="MNILIVKLSAIGDVIHTLPSLAELRRLYPAAHITWVVEEAAADL
                     VLGHPHLDEVIISRRKSWIKDIKAGKIAATLREVRTFVRRLRSRRYDLVIDFHGLLKS
                     SMMVFLSGGKRKLGYDSWQELSGLFLNEKIPEDMNKHAVDRYLDFLRHLGADVGKAEF
                     VLPLTDGTKDQARRLLDEYQLAEKSYIAVNPIAFWETKLWDNGKFAKLADAIRDQLKL
                     DVIFTGN"
BASE COUNT         1224 a         1590 c         1481 g         1088 t
ORIGIN      
        1 cgccatttcc agcccgctga tggattttct gggcggcctg ggcatcgccg ccgtggtttt
       61 ttacggcggc tacaacgtcg tgcagggcca ttccactccc ggcacgtttt tctcctttat
      121 cgccgcgatg ctgatgctct acgaaccgat caagcgcctc accaacgtca acaacaccat
      181 caaccagggc atcgccggcg ccgaccggat tttcagcatc atcgaccgga ccccggacat
      241 cgaggacagg cccggcgccg tcaccctgcc gccggtttcc cggggaatcg acattgaaaa
      301 tgtcacgttc tgctacgaaa cgacgccggt gctgaaaaac atcaatcttt ccatcaaagc
      361 cggggaggtc atcgcctttg tcggcatgag cggcggcgga aaaacatcac tcgtgaatct
      421 gattccccgt ttttacgacg tcaccgaggg ccgcattctg atcgacggcc atgacatccg
      481 caacgtcagt ctgcagtcgc ttcgcggcca gatcgccatc gtgacccagc agaccattct
      541 cttcaacgac accgtccgga acaacatcgc ctacggcgac attcggaaaa cagatgaaga
      601 catctaccgg gctgcccggg cggccaacgc ccacgatttc atccttcgtc tgcccgacgg
      661 ctacgacagc aacatcggcg agctggggac caaactctcc ggcggcgaaa agcagcgcat
      721 ttccatcgcg cgggcgctgc tgaaggacgc cccgattctc atcctggacg aagcgacctc
      781 ctcgctggac acggaagcgg aaatcgaagt ccaggaagcg ctggacaacc tcatgaaggg
      841 gcgcaccacg ctggtcatcg cgcaccgcct ttccacgatc cgcaacgccg accggatcat
      901 cgcgctggtc aacggagaaa tcgtggagga aggaaaccac gacgccctga tggagaaaaa
      961 aggcgaatac taccgattat acaacctgca gttcaaggac gagggaaacg gcaatgatcg
     1021 actggccagg gatatggaat gagaacgatt cgtctcgccg catcagtccg acccgggcca
     1081 ttctttatct gggctccctg gtctaccggc tgattgtctc ttcccgaaac cgtctttacg
     1141 accggcaaat tctgaagttc gtccgcctgg cccgccccgt gatcagcgtg ggaaatctga
     1201 cggtcggcgg aaccggcaaa accccctgcg tgatcgggct ggcaaaaatg ctggcgcgtc
     1261 ggggataccg gccggttgtc ctcagccgcg gctacggcgg gaaaaatcag gcgtccgtca
     1321 atatcgtttc cgacggaaaa aacatcatgc ttggcgcgga agaagccggc gacgaaccgc
     1381 tgctgatcgc ccggtcgctc cccggcattc cggtcctgac cggcgccaag cgggcactca
     1441 ccggacaggc ggccattgag cgattcaacg ccgacgtgct gatctgcgac gacgcgtttc
     1501 agcaccgtca gattttccgg gacatcgata tcgttttgct ggacgcggat aggccgctgg
     1561 gcaacgaccg acttctgccg cgcggcgaac ttcgtgaacc cgcgggaagc cttcgccgcg
     1621 cggactgcat cgtactgacg cgcgccgaca aagccggaac ccctcccgat gaaatcgcgc
     1681 ggatcacccg cgaacggagc atccccgtct tccgctccgt tcaccggctt cgggaaatgg
     1741 tccggccgcg ggagcatctt accctgccgc cggaaaactt tcgcggcaaa agtatctgcg
     1801 ccttctgcgg aatcgcccgc cccgcctcct ttaaaaaatc actgctcgac gcaggaatgc
     1861 acatcctttc atttattgac tttccggatc attacgctta caaacgcgat gacctggagg
     1921 cgattcaaaa gcatttttca acgcaaaacg cggacgcctg gataacgacg gaaaaagacg
     1981 caatgcgcct ggaggaaagt ccggattttt taaaccagct tttcatcgcg cgcatggaaa
     2041 tggaaattca accgtccaca ccgtcctttg acgactttat tctgcgacgg ctgaaagact
     2101 taatacttaa aacttaaaac tatttttcca ttatgagtct taaactgaaa atcggtctgt
     2161 ggatctttcg cgtgataccc cttgcggtca gacgggccgt cttctccggc ctcgcctggc
     2221 tggggtaccg gctgtcggtc aaacaccggc tgattgttct gcacaatctg acccgtgcct
     2281 ttcctgaaaa atcccccgcc gaaattgtgc gcattgccaa agcttcctac cgaagctttg
     2341 gccgggtcgt ggcggaattt tcagaaatca cacgtctgaa tccggacaat gtgcatcaat
     2401 gggtccgcat tcagggcctc gaacattacg acgaggcccg ccgaaaagga aaaggcgttc
     2461 tcctgttcag cgcgcacttc ggcaactggg aaatcggcaa cgccgcaatg gcaatcgcca
     2521 gaaaacctct gatcttcatt taccgcattc tggacagtca atttcttgaa gaggcaatca
     2581 cctatgttcg cgcgacctgc ggcaatattt ccctggacaa ggaaaacgcc atgcggccga
     2641 tgatccgcgc cctgaaaaag ggagaaacca tcaatattct gattgaccag aacgtggcgg
     2701 tttacgatgg aatctttgtg gattttttcg ggcggccggc ctgcacgacc tccggccttg
     2761 ccctgctggc gctccatacc ggtgcgccgg ttctgccggt ttttaccacc cgcatgcccg
     2821 acggaaaata ttggttggag atcggggcgg aagtcgccat ccgcaacacg ggaaaccgcg
     2881 atgccgatgt gcgggaaagc acccagacct tcaccggaat cattgaagag cacatccgca
     2941 aatatcccga acaatggttc tggatgcacc agcggtggaa gacgaagaaa tgccaggcga
     3001 gaatggtgaa tagtgaatag tggatggtgg atgatgatgc tggaagcaaa aaataaactg
     3061 ccgcgggagg gactgaacaa aatcctgatc cgcgggacca actggatcgg cgacgccgtg
     3121 atgacgctgc ccgccgtcgc gtccgtgcgc gcggcctatc cgaaagcgca tctgtccatt
     3181 ctggccaagc cgcccgtctc cgacatctac aaaatgtttt ccgccgccga tgaaattctt
     3241 gcctattcaa gcaaattcga caatgcgccg ggtgtccttc gcctggcgta ttcgctgcga
     3301 tctaaaaagt tcgacgcagc cattttgctg caaaacgcca tcgaagccgc catcatcgca
     3361 ctggccgccg gcattcgcgt gcgtgccggc tacaactcgg acggccgcgg ctttctactg
     3421 acgcatcccg tccggcgtac aacggaaatc cttcgcgtcc atcagatcgg ctactatctg
     3481 gaaatggtca aagcgctggg ctgcgcgaac gtggaacgct cgccgcacct ggaaactttc
     3541 atttccgcgg cggacgctac ggatgtcgtc cggcggcacc ttacggatat ggcaggaccc
     3601 ttcatcggca ttgcgcccgg cgccgcctat ggaccggcaa aacggtggct tccggaaaga
     3661 tttgcgcagg cgggagaccg cctggcaaaa gaattgaacg cgcaggttct tctcttcgga
     3721 ggcagggacg attgggaaac gactgaacag gtgcgcgaac aagcgcaaac caatatgatt
     3781 aatctggctg gccgcgtacc gcttgcggac tcggtttatc tcatgtcgca atgccggctg
     3841 tttctcagca atgattcggg cctcatgcac gtagcaggcg gcttgaatat tccgacggtc
     3901 gccattttcg gctccaccaa tcccgtgacc acctcgcccg cgggagaaaa aacgatcctg
     3961 gtgcgaaagg aaacgccgtg cagtccctgt ttggaaaaaa cctgtccgac ggatttccgc
     4021 tgcatgaccg cgatcaccgt cgaagacgtc gtcgcggcgg cgctgaccct gctgaacggc
     4081 ccggccaacc cgcgccggaa tgaccaaaag agttgacgcg aaatgaaaaa agaagcagcc
     4141 atatttctgg atcgggacgg gaccatcaac gaagaagtcg gctatctcga ctctttggac
     4201 aagctggcca ttttcccatc cgcttttgaa gcgattcgcc tcatcaatca aagcggcctc
     4261 aagacgattg tcgtcaccaa ccaggccggc gtcgcccggg attttttcgg tgaagacttc
     4321 gtggaaacca ttcacgcagc cctgcgggag gcgctgtgtc aaaggggtgc gctgatcgat
     4381 gcgttttatt actgcccgca tcatccgact gagggctttc ccccctatcg gcaaagctgc
     4441 gactgccgca agccggctcc cggcctgttt ctgcgggccg ccatggattg gaatctggat
     4501 ttatccgcat cctggatgat cggagaccgg tacaacgata tcgaggctgc tcaccgggcc
     4561 ggcgccagag gcgttctggt caaaacgggc tacggcgcgg atgcccttgc cggtgcgggc
     4621 cccgatggag aaacgcttgc gggaaagccg gacctcattg cggacgatat ccttcaggcc
     4681 gtccatctca tcctggggag ccgtaaatga atattctgat cgtcaaactg agcgccatcg
     4741 gagatgtgat tcacacgctc ccgtcccttg cggagctgcg ccgtctctat cccgcggcac
     4801 acatcacctg ggttgtagaa gaagctgcgg cggatctggt cctcggccat ccgcatctgg
     4861 acgaggttat catttcccgg cgcaaaagct ggataaaaga tatcaaggcg gggaaaatcg
     4921 cggcgacatt gcgggaagtg agaacgtttg tccgccggct caggagccgc cgctacgatc
     4981 tcgtgattga tttccacggt cttttaaaaa gttcgatgat ggttttttta agtggaggga
     5041 agagaaaact gggatatgat tcctggcagg aactgagcgg attatttctc aatgaaaaaa
     5101 ttccggaaga catgaacaag cacgcggtgg accgctacct ggattttctt cgccatctgg
     5161 gcgcggacgt cgggaaggct gaatttgttc tccccctgac ggacggcaca aaagaccagg
     5221 cccgccgtct gctggatgaa tatcaactgg cggaaaaaag ctacatcgcc gtcaatccca
     5281 ttgcgttttg ggaaaccaaa ctctgggaca acggaaagtt tgcaaaactg gccgatgcca
     5341 ttcgggatca attgaagctg gatgtgattt ttacgggaaa cga
//
LOCUS       DAOSIM010000138         5383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_13625_length_5383_cov_6.121622, whole genome shotgun sequence.
ACCESSION   DAOSIM010000138 DAOSIM010000000
VERSION     DAOSIM010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13625_length_5383_cov_6.121622"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="PLR47_06865"
     CDS             complement(<1..996)
                     /locus_tag="PLR47_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOG12704.1"
                     /translation="MTGREKDWFSKERSRREVLKIAGTAALAGMMGPLAAGRLWAGEK
                     KPNILFILTDDHRYDAFSCMGHPVVKTPNLDRIAREGALFRNTFVTTSLCSPSRASFL
                     TGQYAHTHGVVTNHTMWNDRNVTFLELVKKAGYDTAFIGKWHMPGNRLPQLRGVDEFI
                     TFTKEGGQGVYYDCPLIINGIETPRPGKYITNDLTDFALRFIEKKRKNPFCLYLSHKA
                     VHFGFRPPEHLKTLYEGQDLHLPPESDTWATLTNNNIFVGAPLPMHMLYRNYLRVVAS
                     VDEQVGRLLGLLERMGELDNTVIVYAGDNGHLFGEHGLYDKRNAYEESMRIPFFIR"
     gene            complement(993..1571)
                     /locus_tag="PLR47_06870"
     CDS             complement(993..1571)
                     /locus_tag="PLR47_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12705.1"
                     /translation="MEQKPAFQRIVIPAAAVLALMIVSINLYDASRWWKPPFLQTFFI
                     HLSALGMFAGIWLGAMIANTLAFFRGATFGERLLVCLATPIVWDAKVLSDFIGIFSGA
                     EVFYVCFHAVLMGPIFVALLCMGISEIWCRILYRRRTGDRSVKIMDCKSGLVLIVGFV
                     MTFLLLYNGGHSFYYLYMDLYSRIFLHPGGPQ"
     gene            complement(1697..>2050)
                     /locus_tag="PLR47_06875"
     CDS             complement(1697..>2050)
                     /locus_tag="PLR47_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOG12706.1"
                     /translation="EHNADCYPCDFFVSGEWKIGNLLESRLLTLMNSARRTAFARLKF
                     DRPKECKDCSIASFCMGDCTRFRFHGDSGDQRVSEYCAANQMVYRHIEPHLPEIKKRV
                     AAFRSGRREEHLKTG"
     assembly_gap    2051..2092
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2093..2839)
                     /locus_tag="PLR47_06880"
     CDS             complement(<2093..2839)
                     /locus_tag="PLR47_06880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG12707.1"
                     /translation="MQDRSAPESISLLLKPASGLCNLACDYCFYRRTKALYPGNETKM
                     TRKILETVIRKTLSVDALFNSFCWQGGEPLLMGIDFFRDVVAFQKKHARPGQIIENSL
                     QTNGLLLDAAWCEFLSKENFLVGVSLDGPPEIHNVYRKDYAGRGSFDGVMRGIGLMQQ
                     HEVSFNILCMLTDQNIRSPLELYDFFLSKGFSFLQFIHCFETHPQTGELMPFAVRAEE
                     TGEFYIRLFDAWFEKNFYDVSIRFFEDLLLY"
     gene            3136..3495
                     /locus_tag="PLR47_06885"
     CDS             3136..3495
                     /locus_tag="PLR47_06885"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOG12708.1"
                     /translation="MNRTIVFYATVDGKCPVQDFLDELPGKAAQKVVWVLRLLEDLEM
                     VPVIYFKKLIGTEDIWECRIQHASNIYRILCFFDSRSVIILTHGFQKKSMKTPRQEIE
                     RAETYRRDYLNRKGLKR"
     gene            3492..3767
                     /locus_tag="PLR47_06890"
     CDS             3492..3767
                     /locus_tag="PLR47_06890"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG12709.1"
                     /translation="MSDLKKYIAKRKKTDKAFAEGFEEGYEQFKIGVVLRQAREAAGL
                     TQEELALRLQTQKTAISRIENHAEDIKLSTLDRVAKALGKRLEVNIA"
     gene            complement(3933..4190)
                     /locus_tag="PLR47_06895"
     CDS             complement(3933..4190)
                     /locus_tag="PLR47_06895"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOG12710.1"
                     /translation="MSYRPVYTRKAVKDIGKLEAGLKKRIGTTLLRFKDNPLQYAEPL
                     TDPALGGYRFRIGDYRVIFDMEGDDIVVLRVSHRKEIYRRK"
     gene            complement(4187..4387)
                     /locus_tag="PLR47_06900"
     CDS             complement(4187..4387)
                     /locus_tag="PLR47_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12711.1"
                     /translation="MLNAIKVNLSRQEIIGAVKSMKKKDREAFLEELLATTSPEYLES
                     IKDARADYKAGRVKTHDEVFGH"
     gene            4896..>5383
                     /locus_tag="PLR47_06905"
     CDS             4896..>5383
                     /locus_tag="PLR47_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOG12712.1"
                     /translation="MGESDRTIARARLMGRIKCGQVRAIAGEQGWLDRGLPLPDDEVL
                     GAAFEQKKEHDNNPTHQSLSGAYEEQIRKWVEGNVCMTTIHQTLANQFGFTGSYSSVR
                     RLVQRLKLHQPEATCILDFAPGEAVQVDFGKGPDITDAFTGQTFKTWIFVMTLCFSRH
                     LYA"
BASE COUNT         1313 a         1352 c         1453 g         1223 t
ORIGIN      
        1 gcggatgaag aaaggaattc gcatcgactc ctcgtaggcg ttgcgtttgt cgtagagtcc
       61 gtgctcgccg aagaggtgtc cgttgtcgcc cgcataaacg atgaccgtgt tgtccagctc
      121 gcccatccgt tccagcaacc cgagcagacg gcccacctgt tcgtcaacgg atgccacgac
      181 ccgcagataa ttgcggtaga gcatatgcat gggcagcggc gcgccgacaa aaatattatt
      241 gttcgtgagc gtcgcccagg tgtccgattc gggcggcagg tgaagatcct gcccctcata
      301 gagcgttttc agatgctccg gagggcggaa gccgaaatgc acggccttgt gcgacagata
      361 caggcaaaag gggttctttc ttttcttttc gataaaccga agggcgaaat ccgtcaggtc
      421 gttggtgatg tacttgcccg gccgcggcgt ctcgatgccg ttgatgatca ggggacagtc
      481 gtaataaacg ccctggccgc cttccttggt gaaagtgatg aattcatcga cgcctcgcaa
      541 ttggggcagg cggttgcccg gcatgtgcca tttgccgatg aaagccgtgt cgtagcccgc
      601 ctttttgacg agctccagga aggtcacatt tcggtcgttc cacatggtgt gattcgtgac
      661 cacgccgtgc gtgtgcgcat actggcccgt gagaaaactt gcgcgcgacg ggctgcaaag
      721 cgatgttgtc acaaaggtgt ttcggaaaag ggcgccttcc ctggcgatgc ggtccaggtt
      781 gggggttttc accacgggat ggcccatgca actgaaggca tcgtagcgat gatcgtcggt
      841 caggataaaa aggatgttgg gttttttctc gcctgcccag agccgcccgg ccgcgagcgg
      901 tcccatcatt cccgcaagcg ccgcggttcc cgcgatcttc aggacctctc gccgggaccg
      961 ctctttcgaa aaccaatctt tttcccggcc ggtcattgcg ggcctcccgg atgcaagaaa
     1021 attctggagt acaggtccat atacaaatag tagaaactgt ggccgccgtt atacagcagc
     1081 aggaaggtca tgacaaaacc gacgatcagc accagtcccg atttgcaatc catgattttg
     1141 accgaacggt cgccggtgcg ccggcggtaa aggattctgc accagatttc ggagatgccc
     1201 atgcacaaaa gcgcgacaaa aatcgggccc atcaggacgg catggaagca gacgtaaaat
     1261 acttccgcgc cggagaagat cccaatgaaa tcggaaagga ccttggcatc ccagacgatc
     1321 ggggtcgcca ggcagaccag cagacgctcg ccgaaggttg cgcccctaaa gaaggccagc
     1381 gtattggcga tcatggcgcc cagccagatg ccggcaaaca tgcccagcgc cgaaagatgg
     1441 atgaaaaagg tttgaaggaa cggaggcttc caccagcggc ttgcatcgta aagattgatc
     1501 gagacgatca tcagcgcaag aaccgccgct gcgggaataa cgatgcgctg aaatgcaggc
     1561 ttttgctcca tggaacggtc cttaaaaaat ccacggtcaa ggatggagat tattttctcc
     1621 cgccgaaagc cgcaaatcgc ggctgttcga tttccttcaa agcgtgtgga agtataagag
     1681 caatcccctt tttgtgtcaa ccggttttca gatgctcttc ccttcgcccg ctgcggaacg
     1741 cggcaacgcg ttttttgatt tcgggcagat gcggctcgat atggcgataa accatttgat
     1801 tagcggcaca gtattcactg accctttgat cgcccgaatc cccgtggaac ctgaaccttg
     1861 tgcagtcgcc catgcaaaaa gaagcaatgg agcagtcttt gcactccttc ggccggtcaa
     1921 acttgagccg ggcaaaagcg gtccgccggg cgctgttcat cagggtcaga agccggcttt
     1981 ccagcagatt gccgattttc cactccccgg atacgaaaaa gtcgcaggga tagcagtccg
     2041 cgttgtgctc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatacaaaa
     2101 gcaggtcttc gaaaaaccgg atggaaacat catagaagtt tttttcaaac catgcgtcaa
     2161 aaagccggat atagaattcg cccgtttctt ccgcgcgcac ggcaaagggc atcagctcgc
     2221 ccgtctgcgg gtgcgtttca aaacaatgga tgaattgaag aaatgaaaaa cccttgctta
     2281 agaaaaaatc atacaattcc agaggcgatc ggatattttg atcggtgagc atgcacagga
     2341 tattgaaaga gacttcgtgc tgctgcatca gcccgatgcc ccgcatgacg ccgtcaaagc
     2401 ttccgcgccc ggcgtagtct ttccggtaaa cgttgtgaat ctccggcggg ccgtcgagac
     2461 tgacgccgac cagaaagttt tctttgctga gaaactcgca ccacgcggcg tcgagcagca
     2521 ggccgttggt ctgcagggaa ttttcaatga tctgaccggg ccgggcgtgt tttttttgaa
     2581 aggccacgac atcccggaaa aaatcgatcc ccatcaggag cggttcacct ccctgccagc
     2641 agaaactgtt gaagagggca tcgacggaca gggtttttcg aatgaccgtc tccaaaattt
     2701 tgcgggtcat cttggtttcg tttccgggat aaagcgcttt cgtccgccgg taaaagcaat
     2761 agtcgcaggc cagattgcac aggccggatg cgggcttcag cagaagcgat atcgattccg
     2821 gggcagagcg atcctgcatg agcatacaaa ttccctcgtt tcacaaggcg gtcaatcatt
     2881 caagggtttt ggagacttat atcatgcatc cgtattctct gccattcaaa atcaaaaatt
     2941 cacttgcagg caggcgcgcg agagcttctc tccccggata acaccaagca atccatgcgg
     3001 tatcatcagt caggaaatat ttcgggctat tgggaacatg aaaaaataac agtaagtctc
     3061 catgaaaaga ttatcgtcaa gatcgcctgc aaagatcatc ttgacagaag ttaacatgtg
     3121 ggttaacatg cagccgtgaa ccgaacgatc gtcttctacg caacagtgga cggcaaatgt
     3181 cccgttcagg attttctcga tgagttgccc ggcaaggcgg cacaaaaagt ggtttgggtg
     3241 ctccgtctgc tggaggacct ggagatggtg ccggtcatct atttcaagaa gctgatcggc
     3301 acggaggata tatgggaatg cagaatccaa catgcttcca atatttatcg gatattgtgt
     3361 ttctttgaca gtcgttcagt aatcatactg acccacggct tccagaaaaa gagcatgaaa
     3421 acacccagac aggaaataga aagagctgaa acctatcggc gtgattattt gaatcgtaaa
     3481 ggattaaaga gatgagtgat ctgaaaaaat atatcgcgaa acgaaaaaaa acggataagg
     3541 catttgcgga aggtttcgag gaaggctacg agcaattcaa gatcggggtt gtcctgcgcc
     3601 aggctcgaga agccgcggga ttaactcagg aggaattggc ccttcgtctc caaacccaaa
     3661 agacggcgat ttcaagaatc gaaaaccacg cggaagacat caagttgtct actttggatc
     3721 gggttgccaa agcgctcgga aaacggttag aggttaatat cgcataggtt tcattaacct
     3781 tccataagcc gcgtactcga tcgttgtttc gaatgaatga ctccggcaag gcaacatttt
     3841 tcgttacttt cgcagggtga aagggcggtt ttttgcggac aaaaagaggc cgccggaaat
     3901 tcgaccgctt tatggagaag ccttatccac cctcattttc ttctgtaaat ttccttgcgg
     3961 tggctgacgc gaagcacgac gatatcgtcg ccctccatgt cgaagataac acgataatca
     4021 ccaatgcgaa aacggtaacc gccaagtgcc gggtcggtca gcggttcagc atattggaga
     4081 ggattgtctt tgaagcgaag gagggttgtg ccgatgcgct ttttgagacc ggcttcgagc
     4141 ttgccaatgt ccttgacggc tttccgggta tataccggcc ggtaactcaa tgcccgaata
     4201 cctcgtcatg ggtctttaca cgaccggcct tatagtccgc ccgggcatcc ttgatacttt
     4261 ccaggtattc gggcgaggtt gtcgcgagca attcctccag gaaggcttcc cggtcctttt
     4321 ttttcatcga ttttacggcg ccaataatct cttgccgtga taggttgact ttgatggcgt
     4381 ttaacatgtc aaaacccttt ctattcaact atacaagacc ttcttctttt gctccgtctc
     4441 gccaaaaggc gatctgcttg gctacgtcaa ccatcccgct caatctccgc ggcctaaacg
     4501 gagaactctt ttataagata tagctttttt attgaggaac atcaatggat tttgcagtca
     4561 tgaataagga tttgcggtcc tgcaggtcga gagtggcttg ccgcgcctcg cggctcattt
     4621 ctccctggag tgccagaatc agctcgccga cttggggggt gttactgatc ggtgctatag
     4681 agccaatccg tgatcgccgt aatgaagcca gtatatgatc gccgtaatgg agccacccat
     4741 ttggggtgag gttttctacc gaggggttta aaaaaggtct cggaccatgt gatgaacgtc
     4801 agtatactcg tggcaataca acgctgggag gagctgacat gagcaacagg aggttcgaga
     4861 tgtaccaata tcgtcaagtg atacacagga tgcgcatggg agaaagtgac cggaccattg
     4921 cccgggcaag gctgatgggc cgtataaaat gtggacaggt tcgggccatt gctggggagc
     4981 aggggtggct tgaccgaggg ctgccccttc ctgacgacga ggttctgggg gcggcatttg
     5041 agcaaaagaa agagcatgat aacaatccca cgcatcagtc tttgagtggc gcctatgagg
     5101 aacagattcg caagtgggtt gagggaaacg tctgcatgac cacgattcac cagaccctgg
     5161 cgaatcaatt cggatttacc ggaagctatt cttctgtacg ccgtctggtg cagaggctga
     5221 aactccatca acccgaggcg acctgcattc tggattttgc ccccggcgag gcggtgcagg
     5281 tggacttcgg caaggggccg gacatcaccg acgccttcac cggtcagact ttcaagacct
     5341 ggatttttgt catgaccctg tgcttcagcc gtcatcttta cgc
//
LOCUS       DAOSIM010000139         5346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_13736_length_5346_cov_7.888868, whole genome shotgun sequence.
ACCESSION   DAOSIM010000139 DAOSIM010000000
VERSION     DAOSIM010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13736_length_5346_cov_7.888868"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /gene="ilvC"
                     /locus_tag="PLR47_06910"
     CDS             complement(<1..947)
                     /gene="ilvC"
                     /locus_tag="PLR47_06910"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOG12713.1"
                     /translation="MAKINFGGTVEDVVTSEEFTVKKAQEVLKNEVIAVLGYGVQGPA
                     QAFNMRDNGVSVIIGQSPEDKPYWEKAIADGWVPGQTLFPIEEAVEKATIIQYLVSDA
                     AQMILWPKVKPHLKKGDALYFSHGFSIVYKEQTSVIPPDFVDVIMVAPKGSGTSVRRN
                     FVDGSGINSSFAVHQDATGRAMERTLALGIAIGSGFLFPTTFQMEVYSDLTGERGVLM
                     GALAGMMEAQYAELRRHGHTPSEAFNETVEELTQSLIRLVGENGMDWMYANCSATAQR
                     GALDWRHKFRKAVEPVFAELYQSVAAGRETEIVLRVNSAP"
     gene            complement(992..1477)
                     /gene="ilvN"
                     /locus_tag="PLR47_06915"
     CDS             complement(992..1477)
                     /gene="ilvN"
                     /locus_tag="PLR47_06915"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOG12714.1"
                     /translation="MEKKDHIISILVYNKPDVLARIAGTLGGKGYNIDSLCVNTTTQK
                     DISKIVMTTSGSQATVTRIENQLQRLVDVISVDDLTNVESIHREMVLVRLNRTAHNTQ
                     AVKDAVDANKWKVILSGDAFVIIEITGDKTQIDFALARLEPLGVADITRTGLVALKLE
                     D"
     gene            complement(1514..3217)
                     /gene="ilvB"
                     /locus_tag="PLR47_06920"
     CDS             complement(1514..3217)
                     /gene="ilvB"
                     /locus_tag="PLR47_06920"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOG12715.1"
                     /translation="MKLKGTEILLKALEEEKVDIIFGYPGGAVLDIYDQLYKSNFRHI
                     LVRHEQGAVHAADGYARATGRVGVCLVTSGPGATNTVTGIATANMDSIPLVVFTGQVP
                     TGLIGNDAFQECDITGITRPCTKHNFLVRNIEELAMTIKEAFHIARSGRPGPVLVDLP
                     KNVMAAQTDYDPANVKIRNHEAVYKPAPKKMANVLEMLERAQKPLIMTGGGVILGRAA
                     EELTRLARRFRIPVTGTLMGLGSFPGSDPLWLGMLGMHGTYYANMAVSNCDLLLAVGV
                     RFDDRVTGTIDTFAPNAKIVHIDIDPSSINKNVTVDLPIIGDTKAALSDLLRFLDEHH
                     YEPDSAGRQAWLSEIAQWREKAPLTYCRNGEIIKPQFVIQTLHRLTGGDALITTEVGQ
                     NQMWTAQFFGFDRPNSFITSGGLGTMGFGLPAAIGVKCAFPDRQVVDIAGDGSIQMNI
                     QELATAAQYNIAVKVVLLNNGYLGMVRQWQELFYEKRYSFTDMTYAPDFVKLAEAFGV
                     AGLRATKPDEVEDLLRQGLAMDRPVLMDFRVSREECVYPMVHPGESISQMALGTREMI
                     N"
     gene            complement(3538..>5346)
                     /locus_tag="PLR47_06925"
     CDS             complement(3538..>5346)
                     /locus_tag="PLR47_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOG12716.1"
                     /translation="DEFFLEDRKMINIADVPKLVQYAFVAAEDSRFYKHRGVDLLSIF
                     RALFKNVEAGKIVQGGSTITQQVAKLMYLTPERKYVRKLKEAILSYRIDKYLTKDEIL
                     HLYLNQIYLGHGTYGIESASTGYFGKSARALTLPEAALLAGLPKAPTTYSPFLNFKRA
                     KQRQLYVLARMEEEGFITKDQMNQAAAAPLSLRPMKPKDKVAAYFVEHVRRYVQEKYG
                     ADVLYKEGLTIYTTLNLPAQTAARDALLRGLSEMEGRNKYARGLVQGALYCMDVKTGA
                     IRAMVGGRDFAKSEFNRAVQSRRQPGSAFKPLIYTAAFDKGMTPSTRFVDSPIILEDP
                     SQADGLWKPKNFDEKFQGPITMRTALVQSRNVVTVKILQEIGIDYAISYAANLGIESP
                     LAANLSLALGSAGVTVQEMVRAYGVLVNGGKKVTPYFIRKIVDRTGNVFEETKPESEQ
                     VIDPRIAFMTTYILQDVVESGTGRRVKSIGRPVGGKTGTTNDVRDAWFIGFTPSLITG
                     VWVGFDQEKSLGGHEVGGRAAAPIWLYFMEKALQGKPAETFPVPAGIVFISVDARTGR
                     PAPGGGPGTLQECFLDSAPPGEDGNECFGEREELFR"
BASE COUNT          992 a         1557 c         1574 g         1223 t
ORIGIN      
        1 ggagcgctgt tgacgcgcag gacgatttcc gtttccctgc cggcggcgac ggactggtaa
       61 agctccgcga aaaccggctc caccgccttg cggaatttat gccgccaatc gagcgcgccg
      121 cgctgggccg tcgccgaaca gttggcgtac atccagtcca tgccgttttc tccgaccagg
      181 cggatcaggc tctgcgtgag ctcttccacc gtttcattga aggcctcgct gggcgtgtgg
      241 ccgtggcggc gcagttccgc gtattgcgct tccatcattc ccgccagcgc gcccatcagc
      301 acgccgcgtt cgccggtgag atcgctgtaa acttccatct ggaaggtggt cgggaagaga
      361 aagcccgagc cgatggcgat gcccagagcc agcgtgcgct ccatagcgcg gccggttgcg
      421 tcctggtgaa cggcgaaact ggagttgatg ccgctgccgt caacaaaatt gcggcggacg
      481 ctggtgccgg aacctttggg cgcaaccatg atgacatcga caaaatccgg aggaatcacg
      541 ctggtctgtt ctttgtaaac gatggaaaaa ccgtgcgaga aataaagcgc atcgcctttt
      601 ttcaggtgcg gtttgacttt cggccacagg atcatctgcg ccgcgtcgga cacgaggtat
      661 tggatgatgg tggctttctc caccgcttct tcaatcggga aaagcgtttg gccgggcacc
      721 cagccgtcgg cgatggcttt ctcccagtac ggcttgtctt ccggagactg gccgatgatg
      781 acgctcacgc cgttgtctct catgttgaag gcctgcgcgg ggccctgcac gccgtacccc
      841 agaacggcga tcacttcatt ttttagcact tcctgggctt ttttcacggt gaactcttcc
      901 gaggtcacca cgtcttcaac ggtacctcca aaattgattt ttgccataaa accccctgtt
      961 attttttaag aatcattgtt gatggttaat gttaatcttc aagcttcaaa gctaccaggc
     1021 ccgtgcgggt gatgtcggcg acgcccagcg gctccagccg cgccagggca aagtcgatct
     1081 gcgttttgtc tccggtaatt tcgatgatca caaacgcgtc acccgacaaa atgactttcc
     1141 atttgttggc gtcgacggcg tctttgacgg cctgtgtatt gtgcgccgtc cggttcagcc
     1201 gaaccagcac catttcgcga tgaatggatt cgacgttggt caggtcgtcg acggatatga
     1261 cgtccacgag ccgctggagc tgattttcaa tccgggtgac ggtggcctga gacccgctgg
     1321 tggtcatcac gattttcgag atgtcctttt gcgtggtcgt gttcacgcaa aggctgtcga
     1381 tgttgtagcc cttgccgccc agcgttccgg caatcctggc cagcacatcc ggtttattgt
     1441 acaccaagat tgaaatgatg tggtcctttt tttccatggg taacctctcc gtaccgttat
     1501 tttgccgatc tcatcagttg atcatttccc gggtgcccag agccatctgg ctgatcgact
     1561 cgcccggatg aaccatgggg taaacgcatt cttcgcgcga cacgcgaaaa tccatgagca
     1621 cgggcctgtc catggccaga ccctgccgca aaaggtcttc cacttcgtcg ggcttggtcg
     1681 cccgaaggcc ggcgacgccg aacgcctccg ccagtttcac gaaatccggc gcgtaggtca
     1741 tatcggtaaa ggaatagcgt ttttcataaa agagctcctg ccactggcgc accatgccca
     1801 gatagccgtt gttgagcaaa accaccttga cggcgatgtt gtactgggcg gcggtggcca
     1861 gttcctgaat gttcatctga atgctgccgt cgccggcgat gtccacgacc tgccggtcgg
     1921 ggaaggcgca cttcacgccg atcgccgcgg gcagtccgaa ccccatcgtg cccagtccgc
     1981 cggaagtgat aaaggagttg ggccggtcga atccgaagaa ctgcgccgtc cacatctggt
     2041 tctggccgac ctcggtcgta atgagggcgt cgccgccggt gagccgatga agcgtttgaa
     2101 tgacgaactg cggtttgatg atctcgccgt tccggcagta ggtgagcggc gccttttccc
     2161 gccattgggc aatttcggaa agccaggcct gccgcccggc gctgtccggc tcgtaatggt
     2221 gctcgtcgag gaagcgcagc agatccgaga gggcggcttt ggtgtcgccg atgatcggca
     2281 gatccacggt cacgttcttg ttgatggaag agggatcgat gtcgatatgg acgattttgg
     2341 cgttgggagc gaacgtgtcg atcgtgccgg tcacgcggtc gtcaaagcgg acgcccaccg
     2401 ccagcagcag atcgcagttg ctgaccgcca tgttggcgta ataggtgccg tgcatgccca
     2461 gcatgcccag ccacagcgga tcgcttcccg gaaaggatcc caggcccatg agcgtgccgg
     2521 tgacgggaat ccgaaagcgg cgcgccagcc gggtcaactc ctcggcggcc cggccaagga
     2581 tgacgccgcc gcccgtcatg atcaggggct tttgcgcccg ctcgagcatt tccaggacgt
     2641 tggccatttt tttgggcgcc ggcttgtaaa cggcttcgtg gttgcggatt ttaacgttgg
     2701 ccggatcgta atcggtctgc gccgccatga cgttcttggg cagatccacc agcaccggcc
     2761 ccggccggcc ggagcgggcg atatgaaagg cttccttgat cgtcatcgcc agctcttcga
     2821 tgttgcgcac caggaagtta tgcttggtgc aggggcgcgt aatgccggtg atgtcgcatt
     2881 cctgaaaagc gtcgttgccg atgagccccg tgggcacctg gccggtaaaa accaccagag
     2941 gaatggaatc catgttggcc gtggcaatgc cggtgacggt gttggtggcg ccggggccgg
     3001 atgtgaccag gcaaacgccc accctgccgg ttgcgcgggc gtagccgtcg gccgcgtgca
     3061 ccgcgccctg ctcgtgccgg acgagaatat gccggaaatt gcttttgtag agctgatcgt
     3121 aaatatccag caccgctccg cccgggtagc cgaaaatgat gtccaccttt tcctcttcca
     3181 gcgctttgag cagaatttcc gtgcctttga gcttcattgg cgtgactccc tattcctttc
     3241 tgatttcaga ttccattaaa aaagccgcct gtttcttttg aaaaaagcgg cttggtcttc
     3301 ataaaacaaa gccgcgggct ttttggggcc cgcggcttgt atcttcagtt tttaaagata
     3361 cagagcggca gaccccattc gtacgacgac tactgcgagc cgtcctgcaa tggcctgggt
     3421 ccaaattctg ccgctgtcga aatctttcaa aaacatcatg acatcctttt taattacggc
     3481 tctttttagc gaaaaaacct ttctttgtca agggcattct ccgcgcgggc cgcattttta
     3541 tcgaaacagt tcttctcttt ccccgaaaca ttcattgccg tcttcacctg gcggagcgct
     3601 gtccagaaag cattcctgaa gggtgccggg cccgccgccc ggtgccggcc tgccggtcct
     3661 tgcatccacg ctgatgaaaa cgatgcccgc gggaaccgga aaggtttccg cgggtttccc
     3721 ctgcaaagct ttttccatga agtacagcca gatcggcgcc gccgcgcggc cgcccacttc
     3781 gtggcctccc agggattttt cctggtcgaa gccgacccag acgccggtga tcagggaagg
     3841 ggtgaagccg atgaaccagg catcgcggac gtcgttggtg gttcccgttt tgccgccgac
     3901 cggccggccg atgcttttga cccggcggcc cgtgccgctt tccacgacgt cctgcaggat
     3961 ataggtcgtc ataaaggcga tgcgggggtc gatgacctgc tccgactccg gcttggtttc
     4021 ttcaaaaacg tttccggtgc ggtcgacaat tttccggatg aagtaaggcg tgactttttt
     4081 tccgccgttt accaaaacgc cgtaggcgcg gaccatttcc tgcacggtga cgcccgccga
     4141 gcccagcgcc agggagagat tcgccgccag cggcgattca atgcccagat tggccgcata
     4201 ggagatggca tagtcgatgc cgatttcctg caggatcttg acggtgacga cattgcggga
     4261 ctgcaccaga gccgttctca tggtaatcgg tccctggaat ttttcgtcaa aatttttcgg
     4321 cttccagagc ccgtcggcct gcgacggatc ttccagaata atcggagaat ccacaaaccg
     4381 cgtggagggc gtcatgccct tgtcgaacgc ggccgtgtaa atcaggggct tgaaggcgga
     4441 gcccggctgg cgccgcgact gaacggcgcg gttgaactcg cttttggcga agtcccgtcc
     4501 cccgaccatg gcgcggatcg cgccggtttt cacgtccatg cagtacagcg cgccctgcac
     4561 cagtcctctg gcgtatttgt tccggccctc catttccgaa agcccccgca aaagcgcgtc
     4621 acgggccgcg gtctgcgcgg ggagattcag cgttgtatag atggtgagac cctccttgta
     4681 caaaacgtcc gcgccgtatt tctcctgcac gtagcggcgg acgtgttcga caaagtaggc
     4741 ggcgactttg tctttcggct tcatgggcct caggctcagg ggagcggctg ccgcctggtt
     4801 catctggtct ttcgtgatga agccttcttc ttccatgcgc gccaggacgt agagctgccg
     4861 ttgtttggcc cgcttgaagt tgagaaacgg ggaataggtg gtgggggctt tcggcaagcc
     4921 ggccaggagc gcggcttccg gcagcgtcag cgccctcgcg cttttgccga agtagcctgt
     4981 cgacgccgac tcgattccgt aggtgccgtg ccccagataa atctggttca ggtacaggtg
     5041 gaggatttca tccttggtca ggtatttgtc gatgcggtag gacagaatgg cttccttgag
     5101 ctttcggaca tattttctct ccggcgtgag atacatcagc ttggcgacct gctgggtgat
     5161 ggtgctgccg ccctgcacga ttttgcccgc ttccacgttt ttgaacagcg cgcggaaaat
     5221 gctaagcaga tcgactcccc ggtgcttgta aaagcgggaa tcctccgccg cgacaaacgc
     5281 gtactgaacc agtttcggga cgtcggcgat attgatcatc ttgcggtctt ccaggaaaaa
     5341 ctcgtc
//
LOCUS       DAOSIM010000140         5339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_13756_length_5339_cov_7.554504, whole genome shotgun sequence.
ACCESSION   DAOSIM010000140 DAOSIM010000000
VERSION     DAOSIM010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13756_length_5339_cov_7.554504"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1444
                     /locus_tag="PLR47_06930"
     CDS             <1..1444
                     /locus_tag="PLR47_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12717.1"
                     /translation="LDIIEGYRSKIDDIREERYDNGLRFINRPGEEIHILDSATGEKI
                     PIKGNPRVKLLLSNMGRLSAELIGATIRGLGINARTMPVPDIYTLQMARNHASGKECL
                     PSHLVLGSALKYLSSDQYRKDEIYILFVPTTTGPCRTGQYFVFYENLFRDLRLENVVV
                     FTLEADNSYTELGPEFTKYAWWAAVIGDYMKDVETSLRTCAADPESAMKIYDQIWQKM
                     LLVAEQDITRVLPVLKEIAQTISQIPLARKMEDCPKVLIVGEIYVRRDDFAVDELIGQ
                     FSRHGIVGKVSNVAEWFYYCDHVRRYELHKRLKLLPWHRRFFARELRDIIDWRIEHIY
                     KKRVEKNVKDALGATGLVPRTPHDMRTIMKNAEKHFVSRELHSEIAISSGVAATAMMD
                     GYSGIVNISPFACLIGRVIEGLYTPWARERKYPTISIEIDGNLLPPNVLSKLEIFMLN
                     VKRFRGKGDASTMVERENAQTPAISRKIIR"
     gene            1548..2204
                     /locus_tag="PLR47_06935"
     CDS             1548..2204
                     /locus_tag="PLR47_06935"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOG12718.1"
                     /translation="MSSPATDITGIILSGGRNTRMGVNKAFLEIGPSRMMDQTLDIFR
                     RLFSEILIITNDPLAYLEFDDAAVVTDIYKGKGPLGGIYTGLFYAKNPLAFACPCDMP
                     FLNEDFIAYMISRAGKHDIIVPELPQGYQPLHAIYSRSCLPSIKRLLLMDKLKITGFY
                     RDMRVLTIGEEQIRPFNPDGRLFSNLNTPEDVEKLGGKDLKDSRPPSAGGGPKESKTQ
                     "
     gene            2240..3544
                     /locus_tag="PLR47_06940"
     CDS             2240..3544
                     /locus_tag="PLR47_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOG12719.1"
                     /translation="MFEKEYQEKLTTPQEAVSKIAENALLAHGNLQAEPPALLKAIEN
                     RIRTNDLKKLRVFHLGTSVYASGTILSPELADCVEAYPCFVLTKERGLVYGGLSQFIP
                     NYFHQLRRLIEEYMDEMDVAMTTVSPMDKAGFFSFGTSVDITSIVWKRAKRRIVEVNR
                     HMPRVFGDALVHISDVDQIVENHQPIWESPPAQPDDIDRAIGRIVAEMVPDEATIQLG
                     FGNLPNAVATQLIHHKNLGIHTEAFSPVMVDLVKKGVINGKKKTLHPNKHVYSFALGD
                     HEMMEFMNDNPAFEGYPVSYTNDPDVIARNDNMISINATLQVDLSGQCNSEFLEGAQF
                     SATGGQLDFVRGAFNAKGGKSILTFRSTAKKNTVSRIVPQLDAGSIVTTPRMDTHYLV
                     TEYGAANLKGKCMRDRALAIIGIAHPDFRDQLMREAEKMRLF"
     gene            3894..4718
                     /locus_tag="PLR47_06945"
     CDS             3894..4718
                     /locus_tag="PLR47_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12720.1"
                     /translation="MADSPPQVKTDKEVYAEGEKIQVNFFSAPGSSRDWICIAAAGSP
                     DNEAGDYQYMPHGESQGVLAFAAPVPGKYEVRAYYNYSRNGYVVSARHGFSVAAKGAP
                     VTAPAPSARTAAAPSTARTVEKRVESASVEEFSGADGPANVAVFHFIPLGMDASPYGI
                     TVTNTLINSPGMQQAFLVLPRKDLELFLSANNLQQNDQVDNMVEVGTRLGMNFVIAGT
                     ITKRGSTITTSYKIASMARRGVIHKGQFTSSGEADLIHHVEKMSDTVIDVIRRTVR"
     gene            4821..5012
                     /locus_tag="PLR47_06950"
     CDS             4821..5012
                     /locus_tag="PLR47_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12721.1"
                     /translation="MKRPASLVIAIILFLMAAAQLIRFIVGLPVTAAGQEIPLWPSAL
                     AAVFLAALAVWLLQERKNS"
BASE COUNT         1276 a         1544 c         1418 g         1101 t
ORIGIN      
        1 tctggacatc atcgaaggct accgctccaa aatcgacgac atccgcgagg agcgatacga
       61 caacggcctg cgcttcatca accgtcccgg cgaggaaatc catattctcg acagcgccac
      121 cggcgaaaaa atcccgatca agggaaatcc gcgggtcaaa cttcttttgt ccaacatggg
      181 ccggctctcc gcggagctca tcggcgcgac catccgcggc ctgggcatca acgcccgaac
      241 catgccggtg ccggacattt acacgctgca gatggcgcgc aatcacgcct ccggcaagga
      301 atgcctgccc tcgcatctgg tgttgggcag cgcgctgaaa tatctgtcct ccgaccagta
      361 ccgcaaggat gaaatttaca tcctcttcgt gccgacgacc actggcccct gccggaccgg
      421 ccagtacttc gtgttctatg aaaacctctt ccgggacctg cgcctggaaa acgtcgtggt
      481 cttcacgctg gaagcggaca attcctacac ggagctgggg ccggaattca ccaagtacgc
      541 ctggtgggcg gccgtcatcg gcgactatat gaaggatgtg gaaacctcgc ttcgaacgtg
      601 cgccgccgat cccgagtcag ccatgaagat ttacgatcaa atctggcaga agatgctctt
      661 ggtggccgaa caggatatta cgcgggtttt gccggtcctg aaggaaatcg cgcaaaccat
      721 ctcgcaaata ccgctggcca gaaaaatgga ggactgcccc aaagtgctca ttgtcggcga
      781 aatctacgtc cgtcgcgacg acttcgccgt ggacgaactg atcggccagt tcagccgcca
      841 cggcattgtc ggcaaggtgt cgaacgtcgc cgaatggttc tactactgcg accatgtccg
      901 ccgctacgaa cttcacaagc gcctcaaact gctgccctgg caccggcgct tctttgcccg
      961 ggagttgcgg gacatcatcg actggcgcat cgagcatatc tacaaaaaac gcgtggaaaa
     1021 aaatgtcaaa gacgcgctgg gggcgaccgg cctggtgccg cgcacgccgc atgacatgcg
     1081 gacaatcatg aaaaacgcgg aaaaacattt cgtcagccgg gagttgcatt cggaaatcgc
     1141 catttccagc ggcgtcgccg ccaccgccat gatggacggc tactccggca tcgtcaacat
     1201 ctctccgttc gcctgcctga tcggccgcgt catcgagggg ctgtacacgc cctgggcgag
     1261 ggaaaggaag tatccgacca tttcgatcga gatcgacggc aacctgctgc cgcccaacgt
     1321 cctgtccaag ctggaaattt tcatgctcaa cgtgaaaagg ttccggggca agggggatgc
     1381 ctccaccatg gtggaacgcg aaaacgcaca gacgccggcc atcagccgga aaatcattcg
     1441 gtaaaacctt acggccggct ttgcctcctg ccttgccttt tttcgcttaa tctctttgtg
     1501 cattttatgg tatgccattt acaaaaacga agaaggaaac ggatccattg agcagccccg
     1561 caaccgacat taccggcatc atcctttccg gcggcaggaa cacccggatg ggcgtcaaca
     1621 aggccttcct ggaaatcggt ccctcccgca tgatggatca gacgctcgat atctttcgcc
     1681 ggctcttttc cgaaatcctc atcatcacga acgaccccct cgcctatctg gagtttgacg
     1741 acgcagccgt cgtgaccgac atctacaagg gcaaggggcc gctgggcgga atctacaccg
     1801 gtcttttcta cgcgaagaat ccgcttgcct tcgcctgtcc gtgcgacatg cctttcttaa
     1861 atgaagattt tatcgcgtac atgatcagcc gggcgggaaa gcatgacatc attgtacccg
     1921 agcttcccca aggctatcag cccctgcacg cgatttattc acgaagctgc ctgccctcca
     1981 tcaagcgcct gctgctcatg gacaaactca agatcaccgg cttttaccgc gacatgcgcg
     2041 tgctgaccat cggcgaagaa cagatccggc cgttcaatcc ggacggacgt cttttctcca
     2101 acctcaacac gccggaagat gtggaaaagc tcggcggaaa agatttgaaa gactcccggc
     2161 cgccgtccgc gggcggcggt ccgaaggaat ccaagacgca ataacgacca gggcgtcatg
     2221 cgcctgaaag gggacgaata tgtttgaaaa agagtatcag gaaaaactca ccactcctca
     2281 ggaagccgta tcgaaaattg ccgaaaacgc tttgctggcg cacggcaatc tgcaggcgga
     2341 gcccccggcg ctgttgaagg ccatcgaaaa ccgaatccgc accaatgacc tgaaaaagct
     2401 tcgggtattt catctcggca ccagcgtata tgcctccggg accatccttt caccggaact
     2461 ggccgactgc gtcgaggcct atccttgctt tgtcctgacc aaggaaaggg gtttggttta
     2521 cgggggcctt tcacaattca tcccgaacta ttttcatcag cttcgccgcc tgatcgaaga
     2581 gtacatggat gaaatggatg tcgccatgac gacggtcagc cccatggaca aagcgggctt
     2641 tttttctttc ggcacatcgg tggatattac gtccattgtc tggaagcggg ccaaacgccg
     2701 catcgttgag gtcaaccggc atatgccccg cgttttcgga gacgcgctgg tgcacatctc
     2761 ggatgtggat caaatcgtcg aaaaccacca gccgatctgg gaatctccgc ccgcgcaacc
     2821 ggatgacatc gaccgggcca tcggcagaat tgtcgctgaa atggtcccgg acgaagcaac
     2881 catccagctc ggcttcggga atcttcccaa cgcggtcgcg acgcagctca tccatcacaa
     2941 aaatctgggc attcataccg aagccttctc gcccgtcatg gtggatctgg tgaaaaaagg
     3001 ggtcatcaac gggaagaaaa agacgctgca cccgaacaag catgtctatt cctttgccct
     3061 cggggatcat gaaatgatgg agttcatgaa cgacaacccc gcctttgagg ggtatcccgt
     3121 atcctacacg aacgatccgg acgtcattgc cagaaacgac aacatgattt ccatcaacgc
     3181 caccctgcag gtggatttaa gcgggcagtg caattcggag tttctggaag gagcccagtt
     3241 ttcagcgacg ggcggccagc tcgattttgt gcgcggggcg ttcaacgcga agggagggaa
     3301 atccatcctg acgttccgct ccaccgcgaa gaaaaacacg gtctccagga tcgttcccca
     3361 gctggacgcc ggaagcatcg tcacgacgcc gaggatggat acccactatc tggtcacgga
     3421 atacggcgcc gccaacctga aaggaaagtg catgagggac cgcgctcttg ccattatcgg
     3481 cattgcgcat ccggatttcc gcgatcaact gatgagggaa gcggaaaaaa tgaggctctt
     3541 ctgatgggtt tttgaatcct cagccttcca aacgggtttt tcccccaacg acaaggacga
     3601 tgcggatcgg ggaagaaacc ggaacccacg gaaacaggct gcccttgcgc ttgaattcct
     3661 gtttttcatt ttgatcgcgt tatgctaaac tttgtccgcc gggagggatt ccccgcccag
     3721 gcaaatggaa ggtcggcccg aagaacggac ggaaaacatc aaaccaacgc cacgggagaa
     3781 acactctgcc cggcatcgaa taaaggagaa aacggatgcg gcgaaaccat ctccgaacac
     3841 cgttcattgt ttgcatcacc ctgctgtgtt gttttttttc ggctggaacg gttttggccg
     3901 acagtccccc gcaggtgaaa accgacaagg aggtttacgc cgagggggaa aagattcagg
     3961 taaatttttt cagcgctccc gggtcttcaa gggactggat ctgcatcgct gcggccggtt
     4021 cccccgacaa tgaagccgga gactatcagt acatgccgca cggcgaaagc caaggcgttc
     4081 tggcctttgc agcacccgtc cccggcaaat acgaagtgag ggcctattac aattacagcc
     4141 ggaacggtta tgtcgtctca gctcggcacg gcttctccgt cgcggccaaa ggagcccccg
     4201 taacggctcc ggcgccttcc gccagaaccg cagcggcgcc ttcaacggcc cggactgtgg
     4261 aaaaacgggt ggaaagcgcg tccgtagagg agttctccgg ggcggatggc cccgccaatg
     4321 tggccgtttt tcattttata ccgctcggca tggacgcatc cccctacggc atcacggtga
     4381 ccaataccct gatcaattcg cccggcatgc agcaggcctt tctcgtcctt cccagaaagg
     4441 acctggaatt gtttctttcc gccaataatc ttcagcaaaa cgaccaggtg gacaacatgg
     4501 ttgaggttgg aacaagactg gggatgaatt tcgtgatcgc cggcacaatc acgaagaggg
     4561 gttcgaccat tacaaccagc tacaaaattg ccagcatggc gaggcgcggc gtgattcaca
     4621 aagggcagtt cacgtcctcc ggcgaagccg acctgattca ccatgtcgag aaaatgagcg
     4681 ataccgtcat cgatgtcatt cggcgcacgg tccgctagcg ttgtgcgtcg gaaatctctt
     4741 tgggcgggga tgattccatt tcccggaata aggatttgca acatcatttc cgacattttg
     4801 cattatgaag gaggcaaaaa atgaaacgac ccgcatctct ggttattgca atcatcctgt
     4861 ttttgatggc ggccgcccag ttgatccgct ttatcgttgg ccttccggtg acggccgcgg
     4921 ggcaggaaat ccccttgtgg cccagcgccc ttgccgccgt tttcctggcg gcgctggctg
     4981 tctggctgct tcaggaaaga aaaaattcct aaacgtcatt caaccgcctc gcagcaagct
     5041 gtcgatgtat gaaatgaaca tggttatatc gcagcaagct acggagaata aactctcgtc
     5101 ccgcgggagc gggattcaaa cctgcttaaa tctggaatcg tctaataaat tatgcaatta
     5161 tgtgtattcc tgcataaaaa acttgacaac cccacctctt ccgtgtagag aaatcaaccc
     5221 atgaatgaat gttcattcat gaagcaaagc ggattcggaa tgatgaataa aaccgacaag
     5281 cgtgccgaca tcatggacgt ggccctgaat ctgattgccg agcggggttt tcacgattc
//
LOCUS       DAOSIM010000141         5331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_13792_length_5331_cov_6.674564, whole genome shotgun sequence.
ACCESSION   DAOSIM010000141 DAOSIM010000000
VERSION     DAOSIM010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13792_length_5331_cov_6.674564"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PLR47_06955"
     CDS             complement(<1..246)
                     /locus_tag="PLR47_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12722.1"
                     /translation="MAKREDIKKVLIIGSGPIVIGQACEFDYSGTQACKALRKLGYKI
                     ILVNSNPATIMTDPGMADVTYIEPLNAQTLTEIIENEK"
     gene            complement(375..809)
                     /gene="queF"
                     /locus_tag="PLR47_06960"
     CDS             complement(375..809)
                     /gene="queF"
                     /locus_tag="PLR47_06960"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOG12723.1"
                     /translation="MEKFSKKSDLAGLSLLGSGEKAKPGRKLETFPSHSKRPYTVTLS
                     TQEFTCLCPMTGQPDFAGITIQYIPDRKIVESKSLKLYFWSFRNEGVFHEHLTNIILD
                     DLVAALSPRWCKVTAQFAVRGGIAISVDAEYPEAGKSRNPKR"
     gene            943..1134
                     /locus_tag="PLR47_06965"
     CDS             943..1134
                     /locus_tag="PLR47_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12724.1"
                     /translation="MAETPITPPENTWKCSNCGNTVVQTAPPQTCPVCQQKCEFLNVT
                     CYQPECGFTGMDNRLKPAK"
     gene            1469..2704
                     /locus_tag="PLR47_06970"
     CDS             1469..2704
                     /locus_tag="PLR47_06970"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG12725.1"
                     /translation="MKSWKSLCWMFVFAFVVWGTAAQAAEIGIGFTGPLSGPAAEYGQ
                     DCYNGVDMAVKEINAAGGVTVQGKKYTFKLEKLDDRADPTLAKNNAMRFVSQNKAIAV
                     FNPVATTIGALMAIPEQNFIIAAFSSVHTIMDKKHPMIINPVPNFVAYAKNFSIIAWD
                     KGWRNGAMVVTLGAYGDAWRNVFKAVWEKKGGKILADKPANYYTETDFSAQLTAAIAT
                     KPDFLVVGGPSAPSALVIEQARSLGFKGGFVLIDQAKPDYIAKTLGNKLNLLEGMIST
                     GDIKRLPLPIMPSFMKRYNAAYKGSVVNAENCLNYNMMHIIARAVTAAQSLDAKVVRQ
                     NIVKALPTAGDKFPNELFNIEENGVMNCGTIMQYVNKGQFSKVDYVISFPKSKEEFEK
                     IKKMSKSTDPKQIRYVPLQ"
     gene            complement(2817..4622)
                     /locus_tag="PLR47_06975"
     CDS             complement(2817..4622)
                     /locus_tag="PLR47_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HOG12726.1"
                     /translation="MTREVSVDTVYDNPNNLVDLFEQSVVQYPNNRLFGTKNPASGQY
                     EWVTYKSVADRVDRLRGALKKLGLSKGDKVGVILHNCTEWFVCEQAVHGLGGALVPMY
                     LQELPKVIEYIVRDAGVKFLFVAHAGVYEKVKDFKNSIPTLQEIFVVFGEGENSMKAL
                     EEMGAKNPASSIKPRWSDLANIVYTSGTTGDPKGVMLSHGSLTLCARSGVDGFGLDET
                     MHVVAILPWAHVFGLAADLHDYIYCGGGIAFAESVEKLIQNFQEVKPTGLSVVPRIVN
                     KVYDTIMQGVAADPAKKQFFDAAVAEAVKNRDLPEKTKEFKDYDALVFSQVRKVFGGN
                     LKFFVTGSALIKPEIALFFKDVGQPTFDGYGLTETGPTVSMNSPKYGNKYGTVGTVDR
                     NMHVVIDKSRVGEDSLDGEIIVYGPHVMMGYHNKPQQTAEVMMPDTWNGFPGIRTGDR
                     GWLDEEGFLHITGRFKDEYKLANGKYVHPEGIENEAKLLRWILNIMLYGDGKDYNVAI
                     VVPDFAALQADSKIKPLLKDTLEDSLKEKALTDYLSGEIVAYLRKTYGGYEVPQKFLF
                     VAEDFTVGNGMLTQTMKLKRPLVMKQYGAKLQALY"
     gene            complement(4809..5192)
                     /locus_tag="PLR47_06980"
     CDS             complement(4809..5192)
                     /locus_tag="PLR47_06980"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HOG12727.1"
                     /translation="MSGIESNKDQFRALADNPGQEPFVMLNLLKFKKEGGRESYFRYI
                     RESGPFVEAVGAKVLYFGKPKELLQGSEDWDLLMLVSYPSRKAFLTMANNPDYLKVHE
                     FRAQGVERAVLYATDPVKFREILSE"
BASE COUNT         1193 a         1463 c         1445 g         1230 t
ORIGIN      
        1 cttttcgttt tcaatgatct ccgtcagcgt ctgggcgttg agcggctcga tatacgtaac
       61 gtcggccatg ccggggtcgg tcatgatcgt ggccgggttg gaattgacca gaatgatttt
      121 gtagccgagc ttgcggagcg ccttgcaggc ctgggttccg gagtagtcga attcgcaggc
      181 ctggccgatc acaatcggtc cggagccgat aatcagcact tttttaatgt cttcgcgttt
      241 tgccatgttg tttcctcttg tctttaccgt gggatggtta tttgttgcgc ttcagaaaga
      301 acgattttgc cgggggagta tacgaacaat gaccctgcct gtcaaagaaa ttgtattgaa
      361 agtgcggaaa tcatttaacg ctttggattc cttgacttac cggcttcggg atattccgcg
      421 tccacggaaa tggcgatgcc gccgcgaacg gcaaactggg ccgtcacctt gcaccagcgg
      481 ggggaaagcg cggccacgag gtcgtccaga atgatgttgg tgagatgctc gtgaaaaacg
      541 ccttcgttgc ggaaggacca gaagtagagt ttgagcgatt tcgattctac gattttcctg
      601 tccggaatat actgtatggt gattccggca aagtccggct gaccggtcat gggacacagg
      661 caggtaaact cctgtgtgga cagggtgacg gtatagggtc ttttggaatg gctgggaaac
      721 gtctccagct tgcggccggg cttcgctttt tcacccgatc ccaaaaggga aagtccggcc
      781 agatcgcttt ttttcgagaa cttttccatg catgtctcct gcgcccgaag ggcttttctg
      841 cttaccggaa attctttcgg ttttcaagtt tattcggttg ccaaacggat tttttctgtg
      901 gtaaagattc accactgaaa aaaaatcaag gaggcaacgg tcatggccga aacaccgatc
      961 accccgcccg aaaatacctg gaagtgcagc aactgcggaa acaccgtcgt gcagactgcg
     1021 ccgccgcaaa cctgccccgt ctgccaacag aaatgcgaat ttctgaatgt cacctgctac
     1081 cagccggaat gcggcttcac cggcatggac aaccggctga aacccgcgaa ataagcctcg
     1141 ccggctcgca aatccacgga ctccaaagac cgtccgcctt taaaaaaacg ctgaaaatag
     1201 tgcttgacaa aacagcgctc cctgaatatg tacaaccaac gctcgttgta cgtatgggtc
     1261 catgttcgtt gcgctgcctg aacctgcggg aagggcggca tggctttttg atgcttgccc
     1321 ggacattctc ctgccccgga tcgcccgtat ccccggcgat ccggtttttc taaaggaccc
     1381 aaaggggatc atcgtgatgc gcaagggaaa agcatcagcc gagccggaag cggccggtaa
     1441 tcatttaggg aagaaagggg ataatgcaat gaagagttgg aaatcgttgt gctggatgtt
     1501 cgtctttgcg tttgtggtgt ggggaacggc cgcgcaagcc gcggagatcg ggatcggatt
     1561 tacggggcct ttgagcggac cggccgccga atacggccag gattgctaca acggcgtgga
     1621 catggcggtg aaagaaatca atgccgcggg cggcgtgacc gtccagggca aaaaatacac
     1681 cttcaaactg gaaaagctcg atgaccgggc cgacccgacc ctggcaaaaa acaacgcgat
     1741 gcggtttgta agtcaaaaca aggccattgc cgttttcaat cccgttgcca cgacgatcgg
     1801 cgctttgatg gccattcctg aacagaactt catcatcgcg gccttctcca gcgtccatac
     1861 catcatggac aagaagcacc cgatgatcat caatcccgtc cccaactttg tcgcctacgc
     1921 caaaaacttc tccatcatcg cctgggacaa gggctggaga aacggcgcaa tggtggtaac
     1981 cctgggcgct tacggagatg cctggcgcaa tgtctttaaa gcggtctggg agaaaaaggg
     2041 cggcaagatt ctggccgaca aaccggcaaa ttactacacg gaaacagact tttcggcaca
     2101 gttgacggcg gcgatcgcca ccaagcccga tttcctggtc gtgggcggtc cttcggctcc
     2161 aagcgctctg gtcattgaac aggccagaag cctgggcttc aagggcgggt tcgttctgat
     2221 cgaccaggcc aagccggatt acattgccaa aacgctcgga aataaactga acctgctgga
     2281 aggaatgatc agcacgggcg acatcaaacg cctgcccctg ccgattatgc cttctttcat
     2341 gaaaagatat aatgcggctt acaaaggatc cgtggtcaac gcggaaaact gcctcaacta
     2401 caacatgatg cacatcattg cccgtgccgt caccgcggcg cagtcgctgg atgccaaggt
     2461 ggttcgccag aacatcgtca aggccctgcc cacggccggc gacaaattcc ccaacgaact
     2521 gttcaatatc gaggaaaacg gcgtgatgaa ctgcggcacc atcatgcagt acgtcaacaa
     2581 gggacagttc agcaaggtgg actacgtcat ttcgttcccg aagagcaaag aggaatttga
     2641 gaagatcaaa aagatgagca agagcacgga tccgaagcag atccgctacg tgccgcttca
     2701 gtagcttgtt ctcggtaaat cgagccggcg caatccgcac aggattcacc gggtaaaaaa
     2761 gaacccccgt tctccagaga gagcgggggt tttgtttgtg ccgaactttg cccgggctag
     2821 tagagcgcct ggagctttgc tccgtattgc ttcatgacca gcggccgttt gagcttcatc
     2881 gtctgggtca gcatgccgtt gccgaccgtg aaatcttccg cgacaaacag gaacttctgc
     2941 ggcacctcgt agccgccgta ggtttttcgc agatacgcca cgatttcccc ggaaagataa
     3001 tccgtcaggg ccttctcttt cagggagtct tcgagcgtat ctttcaaaag gggcttgatc
     3061 ttcgaatccg cctgaagagc ggcaaagtcc ggaacgacaa tggcgacgtt gtaatccttg
     3121 ccgtcgccgt agagcatgat gttcaaaatc cagcgcagca gcttggcctc gttttcgatg
     3181 ccttccggat ggacgtactt gccgttggcc agtttgtatt cgtccttgaa gcgtccggtg
     3241 atgtgcagga aaccttcttc atccagccag ccgcggtcgc ccgttcgaat gccgggaaag
     3301 ccgttccatg tatccggcat catcacctcc gccgtctgct ggggcttgtt gtggtacccc
     3361 atcatcacgt gagggccgta aacgatgatc tccccgtcga ggctgtcttc ccccacgcgg
     3421 gatttatcga tgaccacgtg catgtttctg tccacggtgc cgaccgtgcc gtacttgttg
     3481 ccgtatttgg gggagttcat gctgaccgtg ggtcccgttt cggtgagtcc gtagccgtca
     3541 aaggtcggct gtccgacatc cttgaaaaac agcgcgattt ccggtttgat cagcgcactg
     3601 ccggtgacaa aaaatttcaa attgccgccg aaaaccttgc ggacctggga aaagaccagc
     3661 gcgtcgtaat ctttgaattc ttttgttttt tcgggaaggt ccctgttttt gaccgcttcg
     3721 gccacggctg cgtcaaaaaa ctgctttttg gccggatcgg ccgcaacgcc ctgcatgatc
     3781 gtgtcataca ccttgttgac gatgcgggga acaacggaaa gccctgtggg tttgacttcc
     3841 tgaaaattct gaatgagttt ttccaccgac tcggcaaacg cgatgccgcc gccgcaatag
     3901 atataatcgt gaagatcggc ggccaggccg aagacatgcg cccagggcaa aatggccacc
     3961 acatgcatgg tctcatccag gccaaacccg tccacgccgg aacgggcgca cagcgtcagg
     4021 cttccatggg aaagcatgac gcctttcggg tcgccggtgg ttcccgaggt atagacgatg
     4081 ttggccaggt ccgaccagcg aggcttgatg gaggacgctg gattcttggc gcccatctct
     4141 tcgagtgcct tcatcgaatt ttcgccctcg ccgaaaacaa cgaatatctc ttgaagcgtt
     4201 ggaatgctgt ttttgaaatc tttgactttt tcatatacgc cggcatgcgc gacaaaaaga
     4261 aatttcacgc cggcatcccg gacgatgtat tcgatcactt tcggcagttc ctgcaggtac
     4321 atgggaacca gcgcgcctcc caggccgtgg accgcctgtt cgcagacaaa ccattccgtg
     4381 cagttgtgga ggatcacgcc cactttatcg cccttggaaa gtccaagctt tttcaaggcg
     4441 ccgcgcagac gatccacccg gtcggccacg gacttgtagg tcacccattc atactggccg
     4501 gaggccggat ttttggtgcc gaacagccgg ttgtttggat attggacaac gctctgttcg
     4561 aataaatcga ctaaattgtt cggattgtca tacacggtat ccacgcttac ttctcttgtc
     4621 ataatttcac ctctgtacag ggtattgatg tgtgtcccgc taaagctcag ggcgcctgtg
     4681 aacgttttca tcgcagtttg caccgccacg ggatccttgc gcacacttga atcctcggac
     4741 cggaacgatc cgcccggggc tcccgccgcg aattttcaat catcccgttt ggattgcaaa
     4801 gcccgctgct actccgacag gatttccctg aattttacgg ggtccgtcgc atagagcacc
     4861 gcgcgctcca cgccttgggc ccggaattca tgaaccttca gatagtcggg attgttggcc
     4921 atggtcagaa acgccttgcg cgacgggtac gagaccagca tcagcagatc ccagtcctcg
     4981 ctcccctgca gcagctcctt cggttttccg aagtacagca ctttggcgcc gacggcctcg
     5041 acaaaaggtc cggattccct gatatagcgg aagtaggact cccggcctcc ctctttttta
     5101 aacttcagca gattgagcat gacaaacggc tcctggccgg gattgtcggc cagggcccga
     5161 aactgatctt tgttggattc gatgccggac atgatgtttt ctccttcaaa aagatggact
     5221 cgacccgcct tcccggtgtc gcgaatcatt tgaaaaagcg gttcatcagt tccgccactt
     5281 cccggggctt ttcctcctgg agaaagtggg ccgctttgat ctgttcgacg g
//
LOCUS       DAOSIM010000142         5286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_13952_length_5286_cov_9.498949, whole genome shotgun sequence.
ACCESSION   DAOSIM010000142 DAOSIM010000000
VERSION     DAOSIM010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13952_length_5286_cov_9.498949"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PLR47_06985"
     CDS             <1..860
                     /locus_tag="PLR47_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOG12728.1"
                     /translation="APVPVVDVQKDSVMLGGSANVLNTIYAMGGRVFVAGVIGSDDIG
                     RGLIRQLEERRIDTNGILLEKGRPTTLKTRIVAHGQQVVRFDHESRMPITRASAARIL
                     DYVRSLRGQIGAIVISDYSKGVVSRELVEGIQAVAAESKIFLCVDPKSSDFGLYSGVH
                     VITPNHHEAQRAAGMEIVNGDDLLKLGEALLAKFDFQALLVTRGEEGMSLFERRRGIR
                     HTHFPAQAKEVYDVTGAGDTVIGVLALSLAACANLKEATALANHAAGIVVGKVGTSTV
                     SREELIGVL"
     gene            857..1423
                     /locus_tag="PLR47_06990"
     CDS             857..1423
                     /locus_tag="PLR47_06990"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOG12729.1"
                     /translation="MSKPVPAPAVKLIFSVFAPPGKWINDAICRLSVLYGPPDYVSAQ
                     IPFDDTAYYAPEMGQTLVRRFLSMEKLIRPESLPDIKLAANEIESDTRPDSGRRVNID
                     PGYLSQAHLILATGKGYTHRPYLRDGIYADLTLIYQAKKFCSLPWTYPDYGNERLWTM
                     LAAIRSRYLLQLKTAGDGAKPPVLPRKD"
     gene            1426..2304
                     /locus_tag="PLR47_06995"
     CDS             1426..2304
                     /locus_tag="PLR47_06995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOG12730.1"
                     /translation="MIKSMTGYGRVETICDGRNIVVEAKSVNHRFLEIALRTPAALYP
                     LEMEYKKKIGERFRRGRIDVSIRLEGEGAETSRVHLNMEVARDAFEVLSRLRREFNIE
                     EPVSLKNLIGFRDIFSPPAETKLDADFLDRVEQTMREALSLLVRMRQDEGIALFADMH
                     GRLQSIRDIMEAIRLRAPQVVLDYQKRLSDRVRELTAGMEPDPARLAQEVAILADRCD
                     ITEEIVRMGSHIGQFEALLASEEAEGRKIDFLLQEMNREINTIGSKSSDTDIARLVIE
                     VKSELGKLREQAQNIE"
     gene            2309..2932
                     /gene="gmk"
                     /locus_tag="PLR47_07000"
     CDS             2309..2932
                     /gene="gmk"
                     /locus_tag="PLR47_07000"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOG12731.1"
                     /translation="MKEKGICMVISAPSGSGKSTLCRRLLAACPDMEFSVSFTSREPR
                     PNEINGKDYHFISRENFQCRIERGEFVEWTENFGEFYGTSGKVLQDLLQSGKDLLLDI
                     EPRGASQVKNQLQEVVLVFVLPPSLDELLRRLQRRGHETPEAIQKRFDRAESELKEVL
                     WYDYAVINEDLDAAAKQLTAIYQAEKCRTNRLLGMIRRLYPTMKKEA"
     gene            2947..3216
                     /gene="rpoZ"
                     /locus_tag="PLR47_07005"
     CDS             2947..3216
                     /gene="rpoZ"
                     /locus_tag="PLR47_07005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOG12732.1"
                     /translation="MARITIEDSLKVAKTRFGLVSLASKRARQLLKGSRPLMESKNRE
                     IVLALREIAAGKVVYAHPEFLNGSQEDFKPIPDNTEFIGDEEYLE"
     gene            3197..3955
                     /gene="pyrF"
                     /locus_tag="PLR47_07010"
     CDS             3197..3955
                     /gene="pyrF"
                     /locus_tag="PLR47_07010"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOG12733.1"
                     /translation="MKNISNSRPEDRLIFALDAPGVEEASSWIDRLSGSVGLFKVGKE
                     LFTSAGPSIVERVLQKGGRVFLDLKFHDIPNTVARAGEAATALKVDMFNVHASGGSRM
                     IRETVQAVGALAEKKGIERPAVLAVTVLTSLNDQDLAEIGFEKTAGDLVLHLAKMARE
                     AGASGVVASPRDIAALRRALGDDFLIVTPGIRASDEKVADDQKRTLSAFEAISAGADY
                     IVVGRPIRKARDPLEACRRIIREIADGLEARNRA"
     gene            3985..4740
                     /gene="rlmB"
                     /locus_tag="PLR47_07015"
     CDS             3985..4740
                     /gene="rlmB"
                     /locus_tag="PLR47_07015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOG12734.1"
                     /translation="MEAVYGINPIKVLLSRDATPLRKIIIAEGRSGPPLQEILTLAGQ
                     KNIPVEWAPRRRLDQLTGRTDHQGVAGLGEAFAYADLEDVLKNRSPRVSQDLVLILDG
                     ILDPQNLGSVIRSAYCLGANGVVIPSDRSASVTPAAMKASAGSARRLPIARVTNLSRT
                     IDELKSRGFWIYGADARGGKSIREQDFSEPAALVMGSEARGLRPLIRKKCDFFLSIPM
                     AGNFDSLNVAVAAGILQYEILLQRLVKSSGSRA"
     gene            4858..>5286
                     /locus_tag="PLR47_07020"
     CDS             4858..>5286
                     /locus_tag="PLR47_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOG12735.1"
                     /translation="MPVFLWEATTKKGDVRKGEMDVADEMAVRTALRRQGFKSVTVKP
                     KPKDLLENIPFLQGGVEEKNVVVFCRIFSTMIDAGLPLIQCLSILAEQEENKTFAKIL
                     RTIKEDIEGGTSLTDALKKYPKVFDDLFVNLIAAGEAGGIL"
BASE COUNT         1166 a         1412 c         1681 g         1027 t
ORIGIN      
        1 aggcgccggt gccggtggtc gatgttcaga aggattccgt gatgctgggc ggctccgcca
       61 acgtcctcaa cacgatctac gcgatgggcg gcagggtctt tgtggccggc gtgatcggct
      121 ccgacgacat cggccggggt ctgatccgcc agcttgagga gcgcaggatt gacacgaatg
      181 gcattctgtt ggaaaaggga aggccgacca ccctcaagac cagaattgtg gcccacgggc
      241 agcaggtggt tcggtttgat cacgaaagcc ggatgccgat cacccgcgca agcgcggccc
      301 ggattctcga ctatgtccga tccctgcgcg ggcaaatcgg agcgatcgtg atttccgact
      361 acagcaaagg cgtggtttcc cgcgaactgg tggagggcat tcaggccgtc gccgccgaat
      421 cgaaaatttt tctatgcgtc gatccgaagt ccagcgactt cggcctttat tcgggcgtcc
      481 atgtcatcac gcccaatcat catgaggccc agcgagccgc cggcatggaa atcgtcaacg
      541 gagacgatct tctgaaactg ggggaggcgc tcttggccaa atttgatttt caggcgctcc
      601 tggtaacgcg cggcgaagag gggatgagcc tttttgagcg acgccgcggg atccgccaca
      661 cccactttcc ggcgcaggcc aaggaagtct atgatgtaac gggggccggc gacacggtca
      721 tcggcgttct ggcgctgtct ctggccgcct gcgcgaattt gaaggaggcc accgcgctgg
      781 ccaaccacgc ggcgggcatt gtcgtgggca aggtcggaac gtccaccgtg tcgcgggaag
      841 aattgatcgg cgtattatga gcaaaccggt tccggccccg gcggtgaagc tgatcttcag
      901 cgttttcgcg ccgccgggaa aatggattaa cgacgcgatt tgccgcctgt cggtcctgta
      961 cggaccgccg gactatgtga gcgcgcagat cccttttgat gatacggcgt attatgcgcc
     1021 ggaaatggga cagacgctgg tgcggcgttt tctgtcgatg gaaaagctga tccggccgga
     1081 gtcgctgccg gacatcaagc tggccgcgaa tgaaatcgaa agcgatacgc gcccggatag
     1141 cggccgccgc gtgaacattg atcccggcta cctgtcgcag gcgcacctta ttctggccac
     1201 gggcaaaggc tacacgcacc ggccttattt gcgcgacgga atttacgcgg atttgaccct
     1261 gatctatcag gccaaaaagt tttgttccct gccctggacg tatccggatt acggaaacga
     1321 gaggctctgg acgatgttag cggcgatccg gtcccgctat ctgctgcagc tgaaaacggc
     1381 cggagacggg gcaaagcccc ctgttttgcc gcgaaaggat tgaacatgat taaaagcatg
     1441 accggctacg gccgggtgga aacgatctgc gacggccgga acatcgtagt ggaagcaaaa
     1501 tccgtgaatc accggtttct ggagatcgct cttcgaacgc ccgccgcgct ctatccgctg
     1561 gagatggagt ataaaaagaa aatcggggag cgattcaggc gcggccgcat cgatgtttcc
     1621 atccggctgg agggagaagg cgccgaaacc tccagggtgc atctcaatat ggaagttgcc
     1681 cgggacgcct ttgaggttct aagccggctc agaagagagt tcaatattga agagcccgtg
     1741 agcctcaaga acctgatcgg ctttcgggac attttttcgc cgcccgcaga aacgaagctg
     1801 gacgccgatt ttctggatcg ggtcgagcag acgatgcgcg aagccctgtc gctcctggtc
     1861 cgtatgcgcc aggatgaggg gatcgccctg tttgcggata tgcacggcag gcttcagtcc
     1921 attcgggaca tcatggaggc cattcgcctg cgggcgccgc aggtggtgct ggactatcag
     1981 aagcgtcttt ccgatcgcgt ccgggagctg acggcgggaa tggagccgga tccggcccgt
     2041 ctggcgcagg aagtggccat tctggcggac cggtgcgaca tcacggagga aattgtccgc
     2101 atgggaagcc acatcggtca gtttgaggcc ctgctggcaa gcgaggaggc cgaaggccgc
     2161 aagatcgact ttcttcttca ggaaatgaac cgggaaatca acacgatcgg ttccaagagc
     2221 agcgacacgg acattgcccg cctggtgatc gaggtgaaaa gcgagctggg caaattgcgc
     2281 gaacaggcgc agaacatcga gtaggcgtat gaaggaaaag ggaatctgca tggtcatctc
     2341 cgcgccctcc gggtccggaa aaagcaccct ctgccggagg ctgctcgccg cctgtccgga
     2401 catggaattt tccgtttcct tcacgtcgcg ggagccgcgc cccaacgaga tcaacgggaa
     2461 agattaccat tttatttccc gcgagaattt tcagtgccgg atcgaacggg gcgagtttgt
     2521 cgaatggacg gaaaatttcg gcgaattcta cggaacgtcc ggcaaagtgc tgcaggatct
     2581 gctgcaaagc ggaaaggatc tgctcctcga tattgaaccg cgcggggcca gccaggtcaa
     2641 gaatcaattg caggaggtcg ttctggtttt tgtgctgccc ccctctctgg acgaactcct
     2701 aagaaggctg cagaggcggg ggcatgaaac gccggaggcg atccaaaaac gttttgaccg
     2761 ggccgaaagt gaactcaagg aagttctgtg gtatgattac gccgtcatca acgaagacct
     2821 ggacgcggcc gcaaaacagc tgaccgcgat ttaccaggcg gaaaaatgca gaacaaaccg
     2881 tttgctcggc atgatccgcc gtttatatcc aacgatgaaa aaagaggcat aggaggtgct
     2941 cgattcatgg caagaattac gatagaagat tccctaaagg tggcgaaaac ccggttcggc
     3001 ctggtgtcgc tggcgtccaa gcgcgcgcgc cagctgctga aagggtccag gcccttgatg
     3061 gagagcaaaa accgggagat tgttcttgcg cttcgggaaa tcgcggcggg caaggttgtt
     3121 tacgcccatc ctgaattttt gaacgggtcg caggaagatt tcaaaccgat accggacaac
     3181 acggaattta tcggcgatga agaatatctc gaatagccgg ccggaagacc ggctgatctt
     3241 tgcgctggat gcgccggggg ttgaggaggc ttcgtcctgg attgaccgtc tttccggcag
     3301 cgtcggcctg ttcaaggtcg gcaaggaact gttcacgtcc gcagggccgt ccatcgtgga
     3361 gcgcgttttg caaaaaggcg gacgggtttt tctggatctg aagtttcacg atattcccaa
     3421 cacggtggcc cgcgccggtg aagccgcgac ggcgctgaag gtggatatgt tcaacgtgca
     3481 tgcctcgggc ggaagccgga tgattcggga aaccgtccag gccgtggggg cgcttgccga
     3541 aaaaaaaggc attgaacgcc cggcggtgct ggccgtgacg gtgctgacca gtttgaacga
     3601 tcaggacctg gcggaaatcg gttttgaaaa aacggcgggc gatctggttc tgcatctggc
     3661 aaaaatggcc cgtgaggccg gagcgtcggg ggtggtggca tcgcccaggg atattgccgc
     3721 gctgaggcgg gcgctggggg acgattttct gatcgtgacg cccggcatcc gcgcatccga
     3781 tgaaaaagtt gcggatgatc agaaaagaac gctaagcgcc tttgaagcga tttcggcagg
     3841 cgccgattac attgtggtgg gcagacccat ccgtaaggcg cgcgatcccc tggaggcctg
     3901 ccgccggatc attcgggaga ttgccgacgg actggaagcc cgaaaccgcg cgtgacccta
     3961 tcaaaggcag acggaaggcg gatcatggag gcagtttacg gcatcaatcc catcaaagtt
     4021 ctcctttccc gggacgcaac tcctctgaga aaaatcatca ttgccgaagg ccggagcggt
     4081 ccgcctcttc aggaaatcct aaccctggcc gggcaaaaga acatccccgt cgaatgggcg
     4141 ccccgccgcc gcctggatca actgacgggc cggaccgatc atcagggagt agcggggctc
     4201 ggcgaagcgt ttgcgtatgc cgatctcgaa gatgtgctga agaaccgcag cccgcgcgtg
     4261 agccaagatc tcgttttgat tctcgacggc attctggatc cgcagaatct gggatcggtg
     4321 attcgcagcg cctattgcct gggcgccaac ggcgtcgtca ttccgagcga ccggtcggcg
     4381 tccgtcacgc ccgcggccat gaaggcctcc gccggaagcg cccggcgcct gcccatcgcc
     4441 cgcgtgacga atctgtcccg gacgatcgat gaattgaaaa gcagaggttt ctggatttac
     4501 ggggcggatg cccgcggggg aaaaagcatt cgggagcagg atttcagcga gccggcggcc
     4561 ctggtcatgg gaagcgaagc ccgggggctc aggcctttga tccggaagaa atgcgatttt
     4621 ttcctgtcga ttccgatggc ggggaatttc gactctttga acgtcgccgt ggcggcgggg
     4681 atccttcagt atgaaatcct gcttcagcgc ctggtgaaat cctccggttc ccgggcgtga
     4741 agaaggacaa agcattgcgg gcgtgttcga atttaaccgg ttgaaactgc atggaagatg
     4801 gtttatgatg cagccataaa aaaagactcc tgatcaagaa gaaaggaagt ttgcgctatg
     4861 ccggttttcc tttgggaagc gacgacgaag aagggcgatg tcagaaaagg cgagatggat
     4921 gtcgccgatg agatggccgt ccgcacggcg ctgcggcggc agggattcaa atccgtcacg
     4981 gtcaagccaa agcccaaaga ccttctggaa aacattccct ttcttcaggg gggcgtggaa
     5041 gaaaaaaacg tggtggtgtt ctgccggatt ttctccacga tgatcgacgc cggcctgccg
     5101 ctgatccagt gcctgagcat tctggccgag caggaggaaa acaagacgtt tgccaaaatt
     5161 ctccggacga tcaaggaaga catcgaaggg ggaaccagcc tgacggacgc gctgaaaaag
     5221 tatccgaagg tctttgacga cctgtttgtc aacctgatcg ccgccggcga agcgggcggc
     5281 attctg
//
LOCUS       DAOSIM010000143         5253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_14058_length_5253_cov_7.214121, whole genome shotgun sequence.
ACCESSION   DAOSIM010000143 DAOSIM010000000
VERSION     DAOSIM010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14058_length_5253_cov_7.214121"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PLR47_07025"
     CDS             complement(<1..520)
                     /locus_tag="PLR47_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12736.1"
                     /translation="MKSEHHGVLYEFILFGFFLASKIMRHKAHLMGKENLSRMPTPVM
                     FAVTHDSYFEVPSLSRIYYALKPRPDFMVMAKNDFLSGRYLSTNYAKKSKFLKSILLM
                     IDRLGLPLAIFRKLKVAAIDRPFVESFNRKKEDLRREISGQLNQFKESSARGISTLIF
                     PEGTTWGYGGLKK"
     gene            complement(575..1195)
                     /locus_tag="PLR47_07030"
     CDS             complement(575..1195)
                     /locus_tag="PLR47_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOG12737.1"
                     /translation="MRLFPKEESFFELFETLAEKIEEGGSFFLEMTRGRNYTAARVSR
                     LKEIEHEADVIAHRTYERMHKTFLTPIDREDIYALVNKMDEILDHIEGTAVRVYMYKV
                     KKPNDDIVKQAEILFSAIRIIKKIVHGLRDMKNSRMILDGCVEINTLENAGDVAMRSI
                     ITNLFNTETDAIELLKWKEIFERIEEAIDVCEDVSNILEGIVLKHA"
     gene            complement(1245..3854)
                     /locus_tag="PLR47_07035"
     CDS             complement(1245..3854)
                     /locus_tag="PLR47_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG12738.1"
                     /translation="MTARVLIVDDDGNNRLLLETLLKSRGFEVLSAVNGEEGLARARS
                     HHPDLIVSDILMPVMDGYTFCRQLKSDDALKRIPFVFFTASYNTPKDEEFAMSLGAER
                     FVFKPQEPEELIAILEEVLKESRLTPRRAKKPLGEEMEFFRQHNEVLFNQLEKKLLDL
                     EQANERLRMMEEQYRLSFENITDMVLVFDANLTITSVSPSAERILGYRPEEFVGRPID
                     DLKTILTPESFQMAVQNIGQILQGEIITSAVYDVIARDGTRKYLDISGSPLVRDGKID
                     GLVCDARDVTERVLAESARRESEELFGQFMRYFPGSAYIKDSDRRIIYLTGNTEEYFG
                     VKPQEWLGKRSEDIWPEEIALLSRKDDEAVLRGEVVQSISQRPQKDGAHTWITHRFPI
                     HRHNKPPLIGCISLDITGQKQAEENLRESEKRYRELYDFLPIPVYEMDFQSTITSVNH
                     AIYETFGGTEEDFKRGFKAWQLLTPEAVEQSVENIQRLLNGEPLGGTEYRLKRLDGTE
                     FPAIVFSRLIFEQGKPVGLRGAVVNITDRKRTEEVIRESEANFRRSMDDSPLGIRVVT
                     ADGETLYANRAILDMYGYASIEEFQGVPLKQRYTPESYAAYLARRAKRDRGERDQTEY
                     TIDIVTQNGDIRNVRAFRKQIVWNGQPHDQIIYQDITRQIQAEEALRESVGNLRKALG
                     GTIRAIASVVETKDPYTAGHQRRVADIARAIAQEMKLPRERIDGLRMAGIIHDIGKVS
                     VPSEILSSPRKLSSLEFSLIKTHAQSGHDIVREIEFPWPIARIIIEHHERMDGSGYPN
                     ALTGDQILPESRILAVADVVEAMATHRPYRPSLGLEAALDEITQNRGRLYDPEVVDAC
                     LRLFREKDYTIRD"
     gene            4381..>5253
                     /locus_tag="PLR47_07040"
     CDS             4381..>5253
                     /locus_tag="PLR47_07040"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG12739.1"
                     /translation="MKIGKIILLGSMFVLALAAWTSALSAEEVVIGYSGPLSGPAAEY
                     SQDSINGIDMAIREINAAGGISVKGETFTFKLKRLDDRAGMDQAIINARKFVNQDKAP
                     VVFNSLATTIGALLKINEAKGGEFLLVGYSSVHTHHNQGNRLLINPTPDFRSFVKAAS
                     DVAWQKGYRNGTMVVTLGEYGDAWRKHFRTVWEKKGGKIIGDIPVNYYTETDFSAQLT
                     SALATRPDFLLIGGPSATTALAVEQVRSMGFKGGFVLTDQARMDYIADILKNRKLMEG
                     MIGIANVADLPYPAS"
BASE COUNT         1118 a         1488 c         1374 g         1273 t
ORIGIN      
        1 tttttttcag gccgccgtat ccccaggtcg ttccttcggg gaaaatcagc gtcgatatcc
       61 cccgcgcgct gctttccttg aattgattca actggccgga tatttcccgc cgcagatcct
      121 cttttttccg gttgaaagac tccacaaacg gacggtcgat ggccgccact ttcagcttgc
      181 ggaaaatcgc cagaggaagg cccagtcggt cgatcatgag cagaatgctt ttcagaaact
      241 tgcttttttt cgcgtagttt gtggaaagat accggccgct caaaaagtcg tttttggcca
      301 tgaccatgaa atccggccgg ggtttcagtg catagtaaat gcgcgacagg gacggaactt
      361 cgaaatagct gtcgtgggtc acggcaaaca tgaccggagt gggcattcgg gaaagattct
      421 ctttccccat cagatgggcc ttgtggcgca tgattttgga agccagaaaa aacccgaaca
      481 aaatgaattc atacaatacg ccgtgatgct ccgacttcat tttcgcgctc ctttcgcccc
      541 gagagctctg aaaaacggtg aagggctccg ccgatcaggc atgcttcagg acaatgcctt
      601 ccagaatgtt ggaaacgtct tcgcagacgt cgatcgcctc ttcgattcgt tcaaagattt
      661 ctttccattt caagagttca atggcgtccg tctcggtgtt gaagaggttg gtgatgatgg
      721 accgcatcgc aacatcgccg gcattttcca gggtgttgat ttcgacgcag ccgtcgagaa
      781 tcatccggct gttcttcatg tcgcgcagcc cgtgaacaat cttcttgatg atccgaatcg
      841 ccgaaaaaag gatttccgcc tgtttcacga tgtcgtcgtt cggctttttc accttgtaca
      901 tatacacgcg gacggcggtg ccttcgatgt gatccagaat ttcgtccatt ttattgacca
      961 gcgcgtaaat atcttcgcga tcgatgggcg tcagaaaggt tttgtgcatc cgttcgtagg
     1021 ttctatgggc gatgacgtcc gcttcgtgct cgatttcctt cagccgggaa acccgggccg
     1081 cggtgtaatt ccgcccgcgg gtcatctcca ggaaaaaaga tccgccctct tcaatctttt
     1141 cggccagcgt ttcaaagagc tcaaaaaaac tttcttcttt cgggaaaaga cgcatgtgaa
     1201 aaatatctcc tttgattttt tgggcagcgc ctgaaacgat agcgtcaatc ccggatggtg
     1261 tagtcctttt cccggaaaag gcgcaggcag gcatccacaa cctccggatc gtaaagccgg
     1321 ccccggtttt gggttatctc gtccagggcg gcctcaagcc ccagggacgg gcggtaggga
     1381 cgatgcgtcg ccatcgcttc cacaacgtcg gccacggcca ggattctcga ctccggtaga
     1441 atttgatccc cggtcagggc gttgggatac ccggagccgt ccatccgttc atgatgctcg
     1501 atgattatcc gcgcgatggg ccagggaaat tcaatctccc ggacgatgtc atggccggat
     1561 tgcgcgtgcg tcttgatcag gctgaattcc aaagacgaca gtttcctcgg cgaactgagg
     1621 atctcggaag gcacggagac tttcccgatg tcgtgaatga tgccggccat ccgcaggccg
     1681 tcgatccgct cccgcggcag cttcatctcc tgggcgatgg cccgtgcgat atcggcaacc
     1741 cggcgctgat ggcccgccgt atagggatct ttggtttcca cgacggaggc aatggcccga
     1801 atggtcccgc ccagggcttt cctcagattt ccgacacttt cccgcagcgc ttcttcggcc
     1861 tggatttgac gggtaatgtc ctgatagata atctgatcat ggggttgccc gttccagaca
     1921 atctgcttgc ggaaagcccg gacatttcga atgtcgccgt tctgggtgac aatatcgatc
     1981 gtatattcgg tctggtccct ttcgccccgg tctctttttg cccttctggc cagatacgcg
     2041 gcatagcttt ccggtgtata gcgctgtttc aacggaacgc cttgaaactc ttcgatgctg
     2101 gcgtagccgt acatgtccag aatcgcccgg ttggcgtaaa gggtttcacc gtccgcggta
     2161 acaacccgga tgcccagcgg ggaatcgtcc atggaacggc gaaaattcgc ttcgctctcc
     2221 cggatgacct cctccgtcct tttgcggtcg gtgatgttga cgacggcccc ccgaaggccc
     2281 acgggctttc cctgctcgaa aatcaaacgg gaaaagacaa tggcgggaaa ttcggtcccg
     2341 tcgagccgct ttaaccggta ttccgtgccg cctaacggtt ccccattcag cagccgctga
     2401 atgttttcca ccgattgctc aacggcttcc ggcgtcaaga gctgccaggc cttaaagcct
     2461 cttttgaaat cctcctctgt gcccccgaaa gtttcataaa tcgcgtggtt gacagatgtg
     2521 atcgtggatt gaaaatccat ttcatagacc ggaatcggca gaaaatcata caattctcga
     2581 taccgttttt cgctttcgcg aaggttctcc tctgcttgtt tctgccctgt gatgtccaga
     2641 gaaatgcagc cgatcagagg cggtttgttg tgccggtgaa tgggaaaccg gtgcgtgatc
     2701 caggtatgcg ctccgtcctt ctggggcctt tgcgagatgg attgcaccac ttctccccgc
     2761 agaaccgctt catcgtcttt cctggacaaa agggcgatct cttcaggcca gatatcctca
     2821 ctccttttcc ccagccactc ctggggcttt acaccgaaat attcttcagt gttccccgtc
     2881 aaataaataa tgcgccggtc cgaatccttg atgtaggcgc ttcccggaaa ataccgcatg
     2941 aactggccga agagctcttc actctcccgc cgggcgctct ccgcgagcac tcgctccgtg
     3001 acatcgcggg catcacaaac gagtccgtca attttgccgt cgcgcacaag gggcgatccg
     3061 ctgatatcca gatatttgcg ggttccatcc ctggcaatca catcataaac ggcggaggta
     3121 atgatttcac cctgcagaat ctggccgatg ttttgtaccg ccatctggaa ggattccggc
     3181 gtcaggatgg tctttaaatc gtcgatgggc cggccgacga actcttccgg ccggtacccc
     3241 aggatcctct ccgcgctagg cgacacgctc gtaatcgtga gattcgcgtc aaaaacaagc
     3301 accatgtcgg tgatattttc aaagctcagc cggtactgtt cctccatcat ccgcagccgc
     3361 tcgttggcct gttccaggtc aagcagtttc ttttccagct gattgaaaag gacttcattg
     3421 tgctggcgga aaaactccat ttcctcgccg agcggctttt tcgcccgccg gggagtcaaa
     3481 cggctttctt tcaggacctc ttccagaatc gcgatcagtt cttccggttc ctggggtttg
     3541 aagacaaacc gctccgcgcc gaggctcatg gcaaactctt catctttcgg cgtgttgtat
     3601 gaagccgtga aaaacacaaa gggaatccgc ttgagggcgt cgtctgattt caactgccgg
     3661 cagaaggtat atccgtccat gaccggcatc aggatgtcgg aaacaatcag gtccgggtga
     3721 tgtgaacggg ccctggccag gccttcttcg ccgttgaccg cggaaaggac ttcaaacccc
     3781 cggcttttga gaagggtttc caaaaggagc ctattgttgc cgtcgtcgtc cacaattaaa
     3841 acgcgagctg tcattttccg cctttcttcc gttgttcttt gtgagttgcc gtgttttttc
     3901 cgttgcaaga ccggacttca acgttttaag ggactttgaa agcaggtatt tcttgtattg
     3961 gccacaataa ataggaagcc ggttttcctg tcaagaagat ttaataaccc gccctcttct
     4021 ctccagaatt tccggaacga gccggcaaac gcttttcagc cgtcggggtt ggacggacaa
     4081 aacccgatct ctttttctcc ccggtcattg tcttaactca ttctttaaca attgctttca
     4141 tttatttaat attgcaaggc aatttgcaac tcctcctgtc gggatgccgt gcgggccttc
     4201 tgtctttccg cttcctgcca ggaatctctg gaagccctcc gcgccaaacc ggaataccct
     4261 tcgggcctcg ggaaacaggt gcattccggc tgaagctgtg gtatgaatcc ggccgtggcg
     4321 gtcaggattt tgaccacaaa atcaaacagc ggcaataaaa atccgagagg cgaaaaaacc
     4381 atgaagatcg gcaaaataat cttgttaggt tcgatgtttg ttctggcatt ggcggcctgg
     4441 acatcggcgt tgtccgcgga agaagtggtc atcggataca gcggccctct gagcggaccc
     4501 gccgcagaat acagccagga cagcatcaac ggcatcgaca tggccattcg ggaaatcaac
     4561 gcagcggggg gaatctccgt caaaggggaa accttcacct tcaagctcaa acggctggac
     4621 gaccgcgcgg gcatggacca ggccatcatc aacgcccgca aattcgtgaa ccaggacaag
     4681 gcccccgtgg tgttcaactc gctggccacg accatcggcg cccttttgaa gatcaatgaa
     4741 gccaaaggcg gtgaatttct gctggtgggc tattccagcg tgcacacgca ccacaaccag
     4801 ggaaaccggc tgctcatcaa tcccacgccg gactttcgct cctttgtcaa ggccgcctcc
     4861 gacgttgcct ggcaaaaggg gtaccgcaac ggaacgatgg tcgtgaccct gggcgaatac
     4921 ggcgacgcct ggcgcaaaca tttccggacg gtctgggaga aaaaaggcgg caagatcatc
     4981 ggcgacattc ctgtcaacta ctacacggaa acggattttt ccgcgcagtt gacctccgcg
     5041 ctcgcgaccc ggccggactt tcttctcatc ggcggccctt cggcgacgac cgccctggcc
     5101 gtcgagcagg tgcgcagcat ggggttcaaa ggcggttttg tcctgacgga tcaggcccgc
     5161 atggattata tcgcggacat cctcaaaaac aggaagctga tggaaggcat gatcggcatc
     5221 gccaatgtcg cggatcttcc ctaccccgct tcc
//
LOCUS       DAOSIM010000144         5210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_14197_length_5210_cov_7.270999, whole genome shotgun sequence.
ACCESSION   DAOSIM010000144 DAOSIM010000000
VERSION     DAOSIM010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14197_length_5210_cov_7.270999"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1414)
                     /locus_tag="PLR47_07045"
     CDS             complement(116..1414)
                     /locus_tag="PLR47_07045"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOG12740.1"
                     /translation="MANVAVVGTQWGDEGKGKIVDLYTLEADVVARFQGGNNAGHTIV
                     VKGKTTILHLIPSGILHDHKICIIGNGVVFDPKVFLQELKELKEGGIFPSSTKLYVSE
                     KTHVIMPYHRSLDQAREARNSGKKIGTTGRGIGPAYEDKVARTGVRVGDLYEPGLLLE
                     KVRHSLEEKNFLFKNFFGVEPLDPKTVADEYLEYAEKIRPYVADTSLILAGEIQKGKK
                     ILFEGAQGCHLDIDHGTYPYVTSSNTVSGNAACGSGVGPGAISRVVGICKAYTTRVGE
                     GPFPTELKDAVGDHLQKVGQEFGATTGRKRRCGWLDMVLVRQAVRVSGISTLAITKLD
                     VLTGLPKIKICVGYKTAGGECREAVPASLKLFSTCEPVYEEFDGWTEDIASAQNIGEL
                     PVNARKYVQRLKELSGAGIALVSVGAGRDETIQMENPFEN"
     gene            complement(1430..3013)
                     /gene="serA"
                     /locus_tag="PLR47_07050"
     CDS             complement(1430..3013)
                     /gene="serA"
                     /locus_tag="PLR47_07050"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOG12741.1"
                     /translation="MKRVLVSDSMAPEVAAILGNTPGIKVDVKTGMKPDELKAAIRDC
                     DALIVRSATKVTAEIIGAAAKLSAIGRAGAGVDNIDIPAASKRGIVVMNTPGGNTVTT
                     AEHAVAMMMSLARRIPQAAASMKAGRWEKSSFMGNECCNKTLGIIGLGRVGSVVADRA
                     IGLKMNVIAHDPFISPEVAQQMGVKMVSLDELYANSDFITVHSPLSKETKHLVNESAF
                     AKMKKGVFLIHCARGGIVSEKALCDALKSGKVAGAAIDVFEDEPTRNMELVGMDHVIC
                     TPHLGASTDEAQTNVAIAIAHQIAAYFTTGEIKGAVNFPSVSADVMAGIRPYLDLAEK
                     LGTFQAQLLAGAIQEVTLEYSGEILNHNVAPITVSLIKGLLDPILTETVNYINAPVLA
                     KERGIRIIEVKSSETKDYTNLITLTVRTSKETSRTSGAVFGKNDPRIVRINEFSVEIA
                     PEGYMLAVSNEDQPGVIGNLGTTLGSRKVNIARLHLSRDAQSKRALVVLNTDSPVGPD
                     ILDTLRKLPHVVSITPIKM"
     gene            complement(3021..4148)
                     /locus_tag="PLR47_07055"
     CDS             complement(3021..4148)
                     /locus_tag="PLR47_07055"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF002841.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HOG12742.1"
                     /translation="MLNPNFSSGPCSKRPAWSIDALKGAPVGRSHRSALGKERIVKAM
                     NDTRKVLNIPADYLVGILPASDTGAFECAMWSLLGPKPVTVLVWESFSNGWATDVNKQ
                     LKLKPAVVKVDYGKLPDLSKVDWKTDVVFVANGTTSGVKIPNWDWIPADREGLSLCDA
                     TSYAFANKVDWSKIDVLTYSWQKCLGGEAAHGMMILSPKAVERLNSYDPPWPMPKIFR
                     LKKDGKINKAIFEGDVINTPSMLCVEDYLDAMDWAEKIGLDGLVKRSDANLKVVADWV
                     AKTDWIEFLAEDPQTRSNTSVCLSVTHPKVAAMDADAKSKFLKSISSELSKKNVAHDI
                     NSYKEAPAGYRFWCGPTIEEADLKAALAELEKVFTEKVKSL"
     gene            4399..4734
                     /locus_tag="PLR47_07060"
     CDS             4399..4734
                     /locus_tag="PLR47_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG12743.1"
                     /translation="MTENLAKIADLTGAFRLLELVSYQEHSVVSREIIRKPSGTMTVF
                     AFDAGEGLSEHTAPFDALVYLLEGKAEIAIGGKPHPVSEGEMILMPAHKPHALKAITR
                     FKMLLVMIK"
     gene            4752..>5210
                     /locus_tag="PLR47_07065"
     CDS             4752..>5210
                     /locus_tag="PLR47_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG12744.1"
                     /translation="MKFEITPGVDWVGKIDWELRKFHGEEYSTHRGSSYNAYLVRDEK
                     TALIDTVWTPFAKEFVNNLAREIDLNQIDYVIANHAEMDHSGALSALMHRIPDKPVYC
                     TANGLKSLKGHYHKDWNFQVVKTGDKLSLGKKELIFVEAMMLHWPDSMMEY"
BASE COUNT         1056 a         1444 c         1488 g         1222 t
ORIGIN      
        1 ggattaaaag tccgctgctc taccgctgag ctaacggccc cacctgaagc gtttttaaaa
       61 gaacaatgct ctctagcaac tcaccggagg cctgtcaaga agaaactgga cactttcaat
      121 tttcgaaagg attttccatc tgaatggtct cgtcgcggcc cgcgcctacg gaaaccaggg
      181 caatgccggc gccggataat tctttcaggc gctggacgta tttccgagcg tttacgggga
      241 gttcgccgat gttctgcgcg cttgcgatgt cttccgtcca gccgtcgaat tcttcgtaaa
      301 ccggctcgca ggtcgaaaac agcttcaggc tggcggggac tgcttcgcgg cattcgcccc
      361 cggcggtttt gtacccgacg cagattttga ttttgggaag ccccgtcaga acatccagtt
      421 tggtaatggc cagtgtggat atgccggaga cccggacggc ctgacgcacc agcaccatgt
      481 ccagccatcc gcaccgccgc ttgcggccgg tggtggcgcc gaattcctgc ccgactttct
      541 gcagatgatc tcccacggcg tctttgagct ccgtcgggaa aggtccctct ccgacccggg
      601 ttgtataggc tttgcagatt cccaccaccc tcgagatggc gcccgggccg acgccgcttc
      661 cgcacgcggc gtttccggag accgtgttgg aagaggtaac atagggatag gtgccgtgat
      721 cgatatccag atgacatccc tgggcgcctt caaacaggat cttctttccc ttttggattt
      781 ctccggccag aatcagggac gtgtcggcga catagggcct gatcttttcc gcgtactcca
      841 ggtactcgtc cgcgacggtc ttgggatcca ggggctcaac gccgaaaaaa ttcttgaaca
      901 ggaagttttt ttcttccaga ctgtggcgga ctttttccag gagcagcccg ggttcgtaga
      961 ggtcgcccac gcggacgccc gtgcgggcga ccttgtcttc gtacgccggg ccgatgccgc
     1021 ggccggtggt gccgattttt ttgccggagt tgcgggcctc gcgcgcctgg tccaggctgc
     1081 ggtgataggg catgatgacg tgcgtctttt cgctgacata cagcttggtg gaagagggga
     1141 aaatgcctcc ttccttcagt tccttcagct cctgaagaaa aactttcggg tcgaagacca
     1201 ctccgttgcc gataatgcag attttatgat cgtgcagaat gcccgaggga atcagatgga
     1261 gaatggtggt cttgcctttc accacgatcg tatgcccggc gttgttgccg ccctggaacc
     1321 gggcgactac atccgcctcg agcgtataaa gatcgacaat ctttcctttt ccttcatccc
     1381 cccactgggt accgacaacc gcaacattgg ccatgagatt ttttctttcc tacatcttga
     1441 tcggtgtgat ggaaacgaca tgcggcagtt tccgcaacgt gtccagaata tccggcccga
     1501 ccggtgaatc cgtgttcagg acgaccagcg ctcttttcga ctgcgcgtcc cggctcaggt
     1561 gcaggcgggc aatgttgacc ttgcggctgc ccagcgtggt tcccagattg ccgatcacgc
     1621 ccggctggtc ttcgttggag acggccagca tgtatccctc cggggcgatt tccacggaaa
     1681 attcattgat ccggacgatt ctcggatcat ttttgccgaa gactgcgccc gacgtacggc
     1741 tggtctcttt ggaggtccgg acggtcaggg taatcaggtt ggtgtagtct ttggtttccg
     1801 agcttttcac ttcaataatc cggatgcctc tttctttggc gaggaccggc gcgttgatgt
     1861 aattgacggt ttccgtcaga atcggatcca gaagcccctt gatgagggac acggtgatcg
     1921 gggcgacatt gtggttgagg atttccccgc tgtattccag cgtgacttcc tgaatggcgc
     1981 ccgcgagaag ctgagcctga aaagtgccca gcttctccgc cagatccagg tagggacgga
     2041 ttccggccat cacgtccgcg ctcaccgacg ggaaattcac cgcgcccttg atttcaccgg
     2101 tggtgaagta ggcggcgatc tggtgggcga tggcgatggc cacgttggtc tgcgcttcat
     2161 cggtagaggc gcccagatgc ggcgtgcaga tcacatggtc catgccgacg agctccatgt
     2221 ttctggtcgg ctcatcttcg aatacgtcga tggcggcgcc cgcgaccttt ccggatttca
     2281 gggcgtcaca gagcgctttt tcgctgacaa tgccgccgcg ggcgcagtga atcagaaaaa
     2341 cgcccttttt cattttcgca aacgccgatt cgttgaccag atgctttgtt tctttggaaa
     2401 gcggggaatg cacggtgatg aagtcggaat tggcgtacag ttcgtccaga ctgaccattt
     2461 tcacgcccat ctgctgcgcc acttccggag aaatgaacgg atcgtgggcg atgacgttca
     2521 tcttcaggcc gatggcgcgg tcggccacga ccgatccgac gcgccccagc ccgatgatgc
     2581 ccagggtttt attgcagcat tcattgccca tgaaactgga tttttcccag cggcccgctt
     2641 tcatcgaggc ggcggcctgc ggaattctgc gggccagcga catcatcatg gcgacggcat
     2701 gttcggcggt ggtgacggtg ttgccgccgg gcgtgttcat gacgacaatg ccgcgcttgc
     2761 tggcggcggg aatgtcaatg ttgtccactc ccgctccggc ccggccgatg gcggaaagct
     2821 tggcggccgc cccgatgatt tcggccgtaa ccttcgtggc cgaacggaca atcagggcgt
     2881 cgcagtcccg aatggctgcc ttcagctcgt cgggcttcat gccggttttg acatccacct
     2941 tgatgcccgg cgtgttgccc agaatggccg caacttcggg cgccatggaa tcgctgacta
     3001 atacccgttt catagcgttc ttacaaactt ttgacctttt cggtgaagac tttttccagt
     3061 tcggccagcg ccgcctttaa atccgcttct tcaatggtcg gaccgcacca gaaacggtag
     3121 ccggccggcg cttccttgta ggagttgatg tcgtgagcga cgttcttctt gctcagctcc
     3181 gaggaaatgc ttttcaggaa cttgcttttg gcgtccgcat ccattgccgc gactttcgga
     3241 tgcgtcacgg acaggcaaac agatgtgttc gaacgggtct ggggatcttc ggccagaaat
     3301 tcaatccagt cggttttggc cacccagtcg gccacgactt tcaggttggc gtcgcttctt
     3361 ttgaccagac cgtccagacc gattttctcc gcccagtcca tggcgtccag atagtcttcc
     3421 acgcacagca tggagggcgt gttgatgacg tctccttcaa agatggcctt gttgattttg
     3481 ccgtctttct tgaggcggaa aattttcggc atcggccagg gcgggtcata ggaattgagg
     3541 cgctcgacgg ctttcgggct cagaatcatc atgccgtgcg cggcttcgcc gcccagacac
     3601 ttctgccagg aataggtgag cacgtcgatt ttgctccagt cgaccttatt ggcaaacgcg
     3661 tagctggtgg cgtcgcaaag cgagaggcct tcacggtcgg ccggaatcca gtcccagttg
     3721 ggaattttca cgccgctggt ggtgccgttg gcgacgaaaa ccacatcggt cttccagtcc
     3781 accttggaaa ggtccggaag tttgccgtag tcaactttca cgaccgcagg cttcagcttc
     3841 agctgtttgt tgacgtccgt cgcccagccg ttggagaagc tttcccagac cagcacggta
     3901 accggtttgg ggcccagaag cgaccacatg gcgcattcga aagcgccggt atcggaagcc
     3961 ggaagaatgc ccaccaggta atccgccgga atgttcaaaa ctttgcgcgt atcgttcatg
     4021 gctttgacga tccgttcttt tcccagcgcg gaacggtgtg agcggccgac cggcgcgcct
     4081 ttcagcgcgt cgatgctcca cgctggcctc ttgctgcagg ggccggaact aaagttgggg
     4141 tttaacatgg gtaaccatcc ttaaaataaa ttttcttaat gctggcaagt tcataccagc
     4201 attaagcgct tttctcaaga aatttaaacc gtaaaagacc tcccgtgacc gggagtccgg
     4261 acggttgggg gcgaaagcac ccgaaaaaat aaaataacac tttttgactt aagtcaaata
     4321 cattatagtt cgcgcaggtt attaatccat caaacaagat gcggtcttga cggacgtact
     4381 gaaaaaagga gggaaaagat gactgaaaat ttggccaaaa ttgcggatct gaccggcgcc
     4441 tttcgtcttt tggagctggt ttcctaccag gagcactccg tggtcagccg ggaaatcatc
     4501 cggaaacctt ccggaaccat gacggttttt gcctttgacg ccggcgaggg attaagcgag
     4561 cacacggccc cttttgacgc gctcgtttat ctgctggaag gcaaggcgga aatcgctatt
     4621 gggggaaagc ctcacccggt gagtgaaggg gagatgattc tcatgccggc gcacaaaccg
     4681 catgcgctca aggcaattac ccgtttcaag atgctgctgg tcatgataaa ataaaaaaca
     4741 ggaggtgttt tatgaagttc gagattacgc cgggtgtaga ttgggttggg aaaatcgact
     4801 gggagctgcg caagtttcac ggcgaggaat actccacgca tcgcggctcg tcgtacaacg
     4861 cgtatctggt gcgagacgaa aagaccgcgc tgattgatac ggtgtggacg ccttttgcca
     4921 aagagttcgt gaacaatctg gccagggaaa tcgatctgaa ccaaatcgac tacgtgatcg
     4981 ccaatcatgc cgagatggat cactcgggag ccctgtcggc gctgatgcac cgcattcccg
     5041 acaagcccgt ttactgcacg gccaacggcc tgaaatcgct caagggccac taccacaagg
     5101 actggaactt ccaggtggtg aaaaccggcg acaaactgtc tctgggcaaa aaggagctga
     5161 ttttcgtcga agccatgatg ctgcactggc cggattccat gatggagtac
//
LOCUS       DAOSIM010000145         5207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_14206_length_5207_cov_7.765528, whole genome shotgun sequence.
ACCESSION   DAOSIM010000145 DAOSIM010000000
VERSION     DAOSIM010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14206_length_5207_cov_7.765528"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PLR47_07070"
     CDS             complement(<1..300)
                     /locus_tag="PLR47_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(82..84),aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOG12745.1"
                     /translation="MAENLATKEWQPKFVAIVCNWCTYAGADLAGISRIQYPTNVRII
                     RVPCTGRINPFYIVKALQAGADGVLVSGUHPGECHYISGNLVARRKFATLKSFLNY"
     gene            complement(338..4306)
                     /locus_tag="PLR47_07075"
     CDS             complement(338..4306)
                     /locus_tag="PLR47_07075"
                     /inference="COORDINATES: protein motif:HMM:NF009410.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOG12746.1"
                     /translation="MGTSPCKAYCPTHISVQGYVALIAEGKFKEALKLIKQDNPFPIV
                     CGRVCNHPCETACMRGKVDDPIDIMHLKQFVADLDLNSDTRYIPEKKESKGKKVAVVG
                     AGPSGLTCAYYLAIEGYDVDVFEALPVAGGWLAVGIPEYRLPKNVLKAEIKIIEDLGV
                     KIHLNTRIGKDIAFDKLKSDYSAIFIGCGTMKSSKLNIPGEDMKGVVHGVDYLLKVNL
                     GEKVSLGDKVAVIGGGNVAMDAVRTAVRTGSKEVFILYRRTRAEMPASPEEIEEAIEE
                     GIEMKFLVAPKRVVGQDGKVTGIECTRMELGEPDASGRRRPVEIKGSEFIVECDAIVP
                     AIGQEADLSFITKESGVSINKWNNVDFDKITYATNVPGIFTGGDVSTGPQTVVKAVFS
                     GKEAAKSIDRYLRGEDMKAGREKDWTKDLADKADLSNFSKVPREKYPLMKPEDRKTNF
                     KEVGLGFSEAQAIAEASRCLSCGVCSECYQCVEACIAKAIDHDMKVEEETIEVGAIIA
                     SAGFEIFDASKRGEYGYGIYKNVVTALQFERILSASGPFFGHVQRISDGKEPKKIAFI
                     QCVGSRDTSCGNSWCSSVCCMYATKEAIIGKEHAKGLEPTIFYMDIRAHGKDFDRFVN
                     RAKDEYGIRYIRSMPSTIKELQQTNNLLITYVNQDGTLTEEEFDMVVLSVGLMPPKEA
                     KQLAASLGVELEEHGFCKTSLENPVQTSRPGVFVCGAFGGPKDIPETVMEASAAAACA
                     EGLLASRRGTMITPVQNPEEKDMRGQGVRTGVFVCHCGINIGGVVDVPAVRDYAATLP
                     SVVYTADNLFTCSQDTAVKMKEVIEEQNLTRVVVASCSPRTHEGLFQENCEKAGLNRY
                     LFEMANIRDQNSWVHMHEPEAATEKAKDLLRMAVAKAQYLKPLKPGQLSVNHQALIIG
                     GGLAGMTAALSLADQGFASFIVEKEDRLGGNYNHLYKTLEGLDTRAHLKGLLDKVYNN
                     PLITVTTGAKIEKIEGFIGNYKTTVKAKDGEKVFEHGVVIVAIGAYENKPKEYLYGQN
                     AAVKTQRELETMIYEKDAKLASVKNVVMIQCVGSRDKERPYCSRYCCSEAIKNALELK
                     EADPSRDITILYRDIRTFAFKEDYYKKAREANIKFICYEEDRKPEVVAAGDKAEVKVF
                     DPILNEQVSLPADLVALSVGVVPNPENAAIGNMLKVPTNQDGFFMEAHVKLRPVDFAT
                     DGVFMCGMSHSPKFSDETVTQANAAVSRACMILTLDYIEAEGKTAFVNKERCSACGLC
                     EINCPYSAISVNVAEGCAEVNAVLCKGCGVCTASCRMNAVDLNGFNNEEVLAQIYNLN
                     EG"
     gene            complement(4328..4786)
                     /locus_tag="PLR47_07080"
                     /pseudo
     CDS             complement(4328..4786)
                     /locus_tag="PLR47_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
BASE COUNT         1158 a         1415 c         1319 g         1315 t
ORIGIN      
        1 gtagttcagg aaacttttta acgtcgcaaa cttgcgccgc gccaccagat ttcccgagat
       61 atagtggcat tcaccggggt gtcaacccga caccagaacg ccgtctgcgc cggcctgcag
      121 tgctttcacg atataaaagg gattgattcg tcccgtgcag ggtacacgga tgatccggac
      181 atttgtcgga tactgaattc tgctgatacc cgccagatcg gcgcccgcgt aggtgcacca
      241 gttgcagacg atggcaacga atttcggttg ccattctttg gttgctaaat tttcagccat
      301 atattctcct tccaacggcc tgacggtttt tagccgttta gccttcgttt aaattatata
      361 tctgcgccaa tacttcttcg ttgttgaaac cgttgaggtc cacggcattc atgcggcatg
      421 acgccgtgca aacgccgcac cccttacaga gcaccgcgtt gacttcggcg cagccttccg
      481 ccacgttcac gctgatcgcg ctgtagggac agttgatttc acacaaaccg caggcggaac
      541 atctttcctt gttcacgaag gcggtcttgc cttccgcttc gatatagtcc agcgtcagaa
      601 tcatgcaggc gcgcgatacg gcggcgttgg cctgggtgac ggtttcgtcg ctgaatttcg
      661 gtgaatgaga cataccgcac atgaacacac cgtccgtcgc gaaatcaacg ggacgaagtt
      721 tgacgtgcgc ttccatgaag aaaccgtcct gattggtcgg cactttcagc atattgccga
      781 tggccgcgtt ctccggattc ggaacgacac ccacactcaa ggccaccaga tcagcaggca
      841 gactgacctg ttcattgaga atcggatcaa aaacctttac ttctgccttg tctccggcgg
      901 ctaccacttc cggtttgcgg tcttcttcgt aacagatgaa cttgatattc gcttcacgcg
      961 ccttcttgta ataatcttcc ttgaacgcga aggtgcggat atcgcggtac agaatggtaa
     1021 tgtcgcgtga tggatcggct tctttgagtt cgagggcatt tttaatggct tcggaacagc
     1081 agtaacggct gcaatacggt ctttccttgt cgcggctacc cacacactga atcatgacca
     1141 catttttcac agacgcgagc ttggcatctt tttcataaat catggtctca agttcacgct
     1201 gggttttcac tgccgcgttc tggccgtaga gatattcctt gggtttgttt tcataggcgc
     1261 cgatggcaac aatcacgaca ccgtgttcaa acactttttc tccgtctttg gctttgaccg
     1321 tcgtcttata attgccgatg aaaccttcaa tcttctcaat tttcgcgccg gtggtcaccg
     1381 tgatcaacgg attgttgtac actttatcga gaaggccttt cagatgagcc cgtgtatcga
     1441 gaccttcgag tgttttgtaa agatgattgt aattaccgcc cagacggtcc tctttttcca
     1501 ctatgaagga cgcaaatccc tgatcggcca gcgacagagc cgcggtcata ccggccaaac
     1561 cgccgccgat aatcagcgcc tgatgattga cggatagctg accgggtttc aacggcttga
     1621 gatactgggc cttggcgaca gccatgcgca gcagatcctt ggctttttcc gtggccgctt
     1681 ccggttcgtg catatgcacc caggagttct ggtcgcggat gttggccatc tcgaagagat
     1741 aacggttcaa accggctttt tcgcagtttt cctggaacaa tccttcatgc gttcttggcg
     1801 aacaggatgc cacaaccacg cgggtcagat tctgttcctc gatgacctct ttcattttca
     1861 ccgccgtatc ctgcgagcag gtgaagagat tgtcggccgt ataaacaacg gagggcagcg
     1921 tggcggcata atcccgcaca gcgggaacgt caacgacacc gccgatgttg ataccgcaat
     1981 ggcagacaaa tacaccggtg cgcacgccct gtccgcgcat atccttttct tccggattct
     2041 gcacgggtgt aatcatcgta ccgcgacggg aagcaagcag gccttccgcg caggccgcgg
     2101 cggcagaggc ttccataacg gtttcaggaa tatccttggg accgccgaag gcaccgcaga
     2161 caaacacgcc ggggcgggat gtctgaaccg gattctccag cgatgttttg cagaagccat
     2221 gctcttccag ttcaacgccg aggctggcgg ccagttgctt ggcttccttg ggaggcatga
     2281 ggccgaccga cagaacgacc atatcgaatt cttcttcggt cagggttccg tcctgattga
     2341 cataggtaat cagcagattg ttggtctgct gcaattcttt gatggtggac ggcatggaac
     2401 gaatgtagcg gatgccatat tcgtctttgg cgcgattgac aaaccggtcg aaatctttgc
     2461 cgtgcgcacg gatgtccatg taaaagatgg tgggttccag tcctttggcg tgttcctttc
     2521 cgataatcgc ctctttggtg gcatacatac agcaaaccga tgagcaccag ctgtttccac
     2581 aggacgtatc gcgcgacccc acgcattgaa tgaaggcgat tttcttgggc tccttgccgt
     2641 cggaaattct ctggacgtga ccgaagaacg gaccggacgc agaaaggata cgttcaaatt
     2701 gaagcgcggt aaccacgttt ttgtaaatgc cgtaaccgta ttcaccgcgc ttgctggcgt
     2761 cgaaaatttc aaaaccggcc gaggcgataa tggcgccgac ttcaatggtt tcctcttcca
     2821 ctttcatgtc gtgatcgatg gccttcgcaa tacaggcctc cacgcactga taacattcgg
     2881 aacagacacc gcaggacaga cagcggctcg cttcggcaat cgcttgcgcc tcactgaatc
     2941 ccagaccgac ttctttgaaa ttggttttgc gatcctccgg tttcatcagc ggatattttt
     3001 cacggggaac tttggagaaa ttggaaagat cggctttgtc cgccagatcc ttggtccagt
     3061 ctttttcacg gccggctttc atatcctcac cgcgcagata acgatcgatg gatttggccg
     3121 cttccttacc ggaaaaaaca gccttgacga cggtctgagg accggtagaa acgtcaccac
     3181 cggtgaatat gccgggaacg ttggtcgcgt aagtgatttt atcgaaatct acattattcc
     3241 atttattgat cgagacaccg ctttccttag tgatgaacga caaatcggct tcctgaccga
     3301 tggcgggaac gatggcgtcg cattcgacga taaattcgga gcccttgatt tccacgggac
     3361 gacgacgtcc actggcgtcg ggttcaccca gttccatgcg cgtacattcg atgccggtca
     3421 cttttccatc ctggcccaca acgcgtttcg gagccacgag gaatttcatc tcgattcctt
     3481 cttcaatagc ctcctcgatt tcttccggag acgcgggcat ttccgcacgg gtgcgacgat
     3541 agaggatgaa gacttctttc gagccggttc tcaccgcggt gcgcacggcg tccatcgcga
     3601 cgttaccgcc gccgatgacg gccactttgt cgcccaggga aactttttcg cccaggttta
     3661 cttttaacag ataatcaacg ccgtgaacca cgcccttcat atcctcgccg ggaatgttga
     3721 gcttgctgct cttcatcgta ccgcagccga tgaaaattgc gctgtaatca gacttgagtt
     3781 tatcaaaggc aatatctttg ccgattctgg tattgaggtg aatcttcaca cccagatctt
     3841 caatgatctt gatttccgcc ttcagaacat tcttgggcag gcggtattca ggaataccaa
     3901 cggcgagcca gccgcccgct acgggcaggg cttcgaaaac atccacatcg tagccttcaa
     3961 tggccagata ataagcgcag gttaaaccag agggaccggc gccgacgaca gcgaccttct
     4021 tgcccttgct ttctttcttt tccggaatat agcgggtatc gctgttgaga tccaggtcag
     4081 cgacaaattg tttgaggtgc atgatatcga tcggatcatc gacttttccg cgcatacagg
     4141 ccgtctcgca gggatggtta cagacgcggc cgcaaacgat cgggaaagga ttatcctgtt
     4201 tgatgagctt gagcgcttcc ttgaatttac cttcggcaat cagcgccaca tacccctgaa
     4261 cactgatgtg tgtggggcaa taggccttac agggggacgt ccccacctta tcgattgcaa
     4321 aaccgctggg aatggcctgt ggaaaatggc gatagatggc tttgcgttcg cttaaattgc
     4381 cgttaaatcc cgcaggaacc gataccggac aaactttggc acagtcgccg cagcccgtac
     4441 atttatcgag attgacatac cgcggagcgg atgtcatttt cactttgaat tttccgggtt
     4501 cgccctctac cgaattcacg gtccgccggg tcttgatttc aacattggga tggcggccga
     4561 cttcgaccag tttgggagac aatatacagg cggaacagtc attggtcggg aaggtcttgt
     4621 ccaactgagc catgacaccg ccgatgctga ttccgttttc gacgagatag actttcattc
     4681 cggaattggc taaatcgaga gaggcctgaa tgccggcaat gccggatccg accaccatta
     4741 ctgcaccaac tttttcgtta tcttttacca cgtgattaca cctcagtaaa ttatgtaaag
     4801 aagcctggaa aaaccgtctg acacagcgtc cgaccagcgt ccgtcggcta acacactttg
     4861 aattccctgt caagccggga agattgattt aattgaattt tatgtcttaa gcctttgata
     4921 taacaaacat atattttgac cagcgttcac aaaaactgtc cttccccgtt ttggcaagcg
     4981 gcagccgttt ccggccgccg gcttgctttt gtgcgctcct accggggctt tatcaaccgg
     5041 aaaagtgacg gcggacgact ccgcttacgg attttccggc gaacacccgg caagcgctga
     5101 aagatggcgc tcgatcgcct gccagagatc gtcctttccc ttttgcgttt tcgcggaaaa
     5161 aagaagcgcg ggaaagccgg aagtgacccc aagggccttc tcgatgc
//
LOCUS       DAOSIM010000146         5201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_14231_length_5201_cov_7.659930, whole genome shotgun sequence.
ACCESSION   DAOSIM010000146 DAOSIM010000000
VERSION     DAOSIM010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14231_length_5201_cov_7.659930"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PLR47_07085"
     CDS             complement(<1..783)
                     /locus_tag="PLR47_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG12747.1"
                     /translation="MKYDPIAIEEKWQKKWEEEKAFKVTEDPQKKKYYLLEMFPYPSG
                     KIHIGHVRNYTIGDVVARYKRMKGFNVLHPIGWDAFGMPAENAAIEHKIHPSKWTHEN
                     IEHMKKQLKRMGFSYDWDRELATCEPEYYRWEQLFFIWMVEKGLAYKKRSSVNFCKKC
                     DTVLANEQVEGGLCWRCGTEVTEKTLDQWFFKITAYTEELLEYCDKLPGWPERVMTMQ
                     KNWIGKSYGCLVDFPMAEGDGVIQVFTTRQDTLFGATFMLVAA"
     gene            complement(884..1876)
                     /locus_tag="PLR47_07090"
     CDS             complement(884..1876)
                     /locus_tag="PLR47_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOG12748.1"
                     /translation="MNRDIRQFVSHHLGTAEIQYDPILKGGSDRTFYRVRVPSGATFI
                     FMEYGTEVEENAYWAEINRFMTEIGIPVPRIIAYDSRRRFMLLEDLGDADMHSLRAAP
                     WRKRRRCCLMALSAIHRLHRTPLSDVPASLKLCRGYDRSLYTWEHAYFRENFIEAACG
                     LSLSGPQERNWTEEMEALIDRLQKIPECLIHRDFQSQNIMIRNDRPVLLDFQGMRKGN
                     LFYDLASLICDPYVSFSPSERNELIAFYYRIMEPDYSFEKFTGYFWDGAVQRLMQALG
                     AYGFLGLKKNKPAFLHHMANGLKNLLTAVDNAGGLETLNHLLTECKTILARKNY"
     gene            complement(1873..2607)
                     /locus_tag="PLR47_07095"
     CDS             complement(1873..2607)
                     /locus_tag="PLR47_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOG12749.1"
                     /translation="MKHVHPDPADQKIRTAFILGAGLGTRLRPLTQLTPKPLLPVGGR
                     PIITHAMEHLRAAGVTRFIINTHHCPEKYLEAFPEGRWEDVPITFRHEPVLLDTAGGI
                     KNIEDLIGQDRRIIVYNGDIITNLPLEPLLKRHTELCTEATLALRSRGPLTNVHIDSQ
                     GFICDMRNTLHNPGVQACLFAGIYILETAFLRRLAAGKIESIVLPLIESIRQNPRSVG
                     GIVIDEGFWHDLGTMEEYRTLRDLTL"
     gene            2826..3614
                     /locus_tag="PLR47_07100"
     CDS             2826..3614
                     /locus_tag="PLR47_07100"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOG12750.1"
                     /translation="MTNIFRRGTGKRKRQGETFSPETERGGRNDFFRVRCLFFFACAI
                     LLMTGSVPATSHAGEAASQPASKEGQSPPPVAGNSAFAAADPDGQGAAAESVKKPGVL
                     RSNLLNISIRGRFSGLDVLGGTATEEFQEFDAAAQFRLPWGWYSQPGWGGELRLMAGA
                     GVLYGAGETALAVSLIPMLAFGSKDGRFSLDMGAGPALLSRHRFGDQDYGGYFQFALT
                     AGLSVPVFRGFGVGYRFLHYSDAALYGHHTTGADFHMLEIHYRF"
     gene            3769..4125
                     /locus_tag="PLR47_07105"
     CDS             3769..4125
                     /locus_tag="PLR47_07105"
                     /inference="COORDINATES: protein motif:HMM:NF037472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HOG12751.1"
                     /translation="MKKLSIALLLIVLALGGQTAQAMSGHDLVDGMKSYLKAEQDYTR
                     ADYLLAGNYMGYVQGVAEATVSDYSLPGGVTPDALYKVVADFLQKNPEKWDKPAAFLV
                     RSALLAAYPKYLIRNQ"
     gene            4143..4841
                     /locus_tag="PLR47_07110"
     CDS             4143..4841
                     /locus_tag="PLR47_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HOG12752.1"
                     /translation="MKKLWLAGALILLFSGCPPAHKTSPEEQFKKSFPQHSYETMKQT
                     SARGVYEVYNGSQIYYYLPDGDMLFVGSMISKDGTNLTQESTGKKMTSKMAGLSLDKA
                     LKIGEGKTSVVEFIDPNCHYCRQSHAFFAGRKKEVTLYVFFYPLSRDAEDKIRHIFCA
                     ADKAEEYGKVLSGKLDGKDQLNLCTDRNAEDRIKAHREASAKIGVRATPMFFIKGRLV
                     TGFDRPVIEALLKD"
     gene            complement(4881..>5201)
                     /gene="metG"
                     /locus_tag="PLR47_07115"
     CDS             complement(4881..>5201)
                     /gene="metG"
                     /locus_tag="PLR47_07115"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HOG12753.1"
                     /translation="PEIDYDEFSKVDLRVAKILSAEAVPKSNKLVKLRIDIGEERTIV
                     AGIGKDYKPQELVGKSIVIVANLKPAKLMGVESHGMLLATDSPEGLTLIGFDREPKTG
                     AKVR"
BASE COUNT         1120 a         1373 c         1514 g         1194 t
ORIGIN      
        1 ggccgccacc agcatgaagg ttgcgccgaa aagcgtgtcc tggcgcgtgg tgaagacctg
       61 aatgacgccg tcgccttccg ccatcggaaa atcgacgagg cagccgtagc ttttgccgat
      121 ccagtttttc tgcatcgtca tgacgcgctc cggccagccg ggaagcttgt cgcagtattc
      181 caaaagctcc tcggtgtagg cggtgatttt gaaaaaccac tgatcgagcg ttttttccgt
      241 gacttccgtg ccgcagcgcc agcacagacc gccttcgacc tgttcattgg ccagcaccgt
      301 gtcgcacttt ttgcagaaat tgacgctgga gcgtttcttg taggccaggc ctttttcaac
      361 catccagata aagaaaagct gttcccagcg gtagtattcc ggttcgcagg tggccaattc
      421 gcggtcccag tcgtagctga atcccatgcg cttgagctgc ttcttcatgt gctcgatgtt
      481 ttcatgcgtc cacttggacg gatgaatctt gtgctcgatg gcggcgtttt ccgccggcat
      541 gccgaaggcg tcccagccga tgggatggag aacgttgaat cccttcatgc gtttgtagcg
      601 ggccaccacg tcgccgatgg tgtaattgcg gacatgtccg atatggattt ttcccgacgg
      661 ataggggaac atttccagga ggtagtattt ctttttctgc ggatcttccg tgactttaaa
      721 agctttttct tcttcccatt tcttttgcca tttttcttca atggcgatcg gatcgtactt
      781 cataacggtg tttttctccc aaaagagttc aataatcggg gatttgctta acatatcgat
      841 acgatgagcg caacgccttt atcgaggagg aaggcagacg attttaataa tttttacggg
      901 caaggatggt tttgcattcc gtcagcagat ggttcagtgt ttcgagtccg cccgcgttgt
      961 cgacagccgt cagcaaattc ttcaggccat tggccatatg gtggagaaat gcaggtttgt
     1021 tttttttgag gccgagaaat ccgtaagcgc ccagcgcctg catcagccgc tggactgcgc
     1081 cgtcccagaa atagccggtg aatttttcaa agctgtaatc cggctccatg atccggtaat
     1141 aaaaagcaat gagttcgttt cgttccgacg gggagaaaga gacgtagggg tcgcagatca
     1201 gggaagccag atcgtaaaaa agattgccct tccgcatgcc ctggaaatca agcaataccg
     1261 gccggtcgtt tctgatcatg atattctggg actggaaatc ccggtggatc aggcactccg
     1321 ggattttttg caggcggtcg atgagcgcct ccatctcttc cgtccagttg cgctcctgcg
     1381 gtccggaaag cgaaaggccg catgcggcct cgatgaaatt ttccctgaag taggcgtgtt
     1441 cccaggtgta aagcgaccgg tcataccccc ggcacagttt cagggaagcc ggcacgtcgg
     1501 aaagcggagt ccggtgaagc ctgtggatcg cggaaagcgc catgaggcag cagcgtctcc
     1561 gcttgcgcca gggagccgcg cgcagggaat gcatatccgc gtcgccgagg tcttccagga
     1621 gcatgaaccg acgccgggag tcgtaggcga tgatgcgggg aaccggaatg ccgatctccg
     1681 tcatgaagcg gttgatttcc gcccagtagg cgttttcttc gacctcggtt ccatattcca
     1741 tgaaaatgaa ggtggcgccg gaaggcacgc gcacacggta aaaggtgcgg tcggagccgc
     1801 ccttgagaat cggatcgtac tgaatctccg ccgtgcccag atgatggctt acaaattgtc
     1861 gaatgtcccg gttcatagcg tcagatcgcg tagtgtacgg tattcttcca tcgtgcccag
     1921 atcgtgccag aagccttcat cgatgacgat tccgccgacg gaccggggat tttgccggat
     1981 gctctcgatg agcggcagca caatcgattc gattttcccc gccgcaagcc gccgcaaaaa
     2041 ggccgtttcc agaatataaa tgcccgcgaa gaggcaggcc tggacgcccg ggttgtgcaa
     2101 agtgtttctc atatcgcaga taaatccctg tgagtcaata tggacattgg tcagcggccc
     2161 ccggctgcgc agcgccagtg tcgcctctgt gcaaagctcc gtgtgtcttt tcagcagggg
     2221 ctcaagcggg aggttggtga tgatgtcgcc gttgtaaacg atgatgcggc ggtcttgccc
     2281 gatcaaatcc tcgatgttct tgatgccgcc cgccgtgtcc agaagcaccg gttcgtggcg
     2341 aaacgtgatg ggaacgtcct cccatcggcc ctccgggaac gcttcgagat atttttccgg
     2401 gcagtgatgc gtgttgatga taaaccgagt tacaccggcg gcgcgcaaat gctccatcgc
     2461 atgagtgatg atcgggcgtc cgccgaccgg cagcagcggt ttgggcgtga gctgtgtgag
     2521 cggccgaagc cttgttccca gccccgcgcc cagaatgaag gccgtgcgga ttttctggtc
     2581 cgccggatca ggatgaacat gtttcatgac cgagtattca gcaggacttt gttcaaaagt
     2641 caagcttcgc gaactctttc accgggccgg aaatttgtgt tatagaaaag acagaaatca
     2701 atgaatggtg accgttttca tggtgaatag tggatagtgg atggtgggtg gtaaaaagct
     2761 cgtagatcat ggctcatagc aaaaagcagc gagtggcgag tgaagtcgta gggcggggag
     2821 gcgggatgac gaatattttc aggcgcggca cgggcaaacg caaaaggcag ggagagacct
     2881 tttccccgga gacagaacgc ggcgggcgga acgatttttt ccgtgttcgt tgccttttct
     2941 tttttgcctg tgccatcctg ctgatgaccg gctccgttcc ggcgacgtcc cacgccggtg
     3001 aggccgcgtc tcaaccggcg tccaaagaag ggcaatcccc gccgccggtt gcggggaatt
     3061 cagcattcgc cgccgctgat cccgacggtc agggcgccgc cgcggaatca gtgaaaaagc
     3121 cgggggttct tcgatcgaac ctgttgaaca tcagcattcg gggccgcttt tcgggcctgg
     3181 atgttttggg cggaacggcg acggaagaat ttcaggaatt tgacgcggcg gcgcagttcc
     3241 ggctgccctg gggatggtat tcccaacccg gctggggagg cgagctgagg ctgatggccg
     3301 gcgccggcgt tttatacggc gccggagaaa ccgcgctggc tgtttccctg attcccatgc
     3361 tggctttcgg cagcaaagac ggaaggttca gtctggatat gggggcgggc ccggccctgc
     3421 tcagcaggca tcgcttcggg gatcaggact acggcggata ctttcagttt gcgctgaccg
     3481 cgggcctcag cgtgccggtc ttcagaggat tcggcgtcgg ctaccggttt ctgcattatt
     3541 ccgatgcggc cctctacgga catcacacga cgggagcgga ttttcacatg ctggagatcc
     3601 actaccggtt ttgaggggtc gggagcgcgg acagtccgcg ccgacccgac gactttcaaa
     3661 aacgtatcta ttgctttcca taagcgttgt tgacggatga ggaagattat gcgacattcc
     3721 ggccggcgca tgtgaacgtg aaagaaaata atcaaacagg atgtctctat gaaaaaacta
     3781 tccattgccc tgttgttgat cgtgctggct cttggcggac agaccgcaca ggccatgagc
     3841 ggtcatgatc ttgtcgatgg aatgaagtcc tatttaaaag ccgagcagga ctacaccaga
     3901 gcggattacc tgctggctgg aaactacatg ggatatgttc agggcgtggc cgaagctacg
     3961 gtttccgact actctttgcc cggcggcgtc acacccgacg cgctgtataa agtcgttgcc
     4021 gatttcctgc aaaaaaaccc ggaaaagtgg gacaaaccgg ccgcgttcct ggtgcgcagc
     4081 gccctgcttg ccgcctaccc gaaatatctg attcgaaacc aataacgcaa aggagttttt
     4141 ccgtgaaaaa attatggctt gcgggagcgc tgattctgct cttttccgga tgcccgcccg
     4201 ctcacaagac atcgccggaa gagcagttta aaaaaagctt tccccagcat tcctacgaaa
     4261 cgatgaagca aacctccgca aggggcgtct atgaggtcta taacggcagc cagatttatt
     4321 attatcttcc ggacggcgac atgcttttcg tgggcagcat gatttccaaa gacgggacca
     4381 atctcacgca ggagagcacc gggaaaaaaa tgacatccaa aatggcgggc ctgtcgctcg
     4441 acaaagccct gaagatcggc gaagggaaaa cctccgttgt ggaattcatc gatccgaact
     4501 gccattattg ccgccagtcc catgcgtttt ttgccggacg caagaaggag gtcaccctgt
     4561 atgtcttttt ctaccctttg tccagggacg cggaagacaa aatccggcat attttctgcg
     4621 ccgccgacaa agccgaagaa tacggaaaag ttttgtcggg gaagctggac gggaaggatc
     4681 agttgaatct atgcaccgac cggaatgccg aagaccggat caaagcgcac cgggaggcat
     4741 ccgcgaaaat cggcgtgcgc gcgacgccga tgttttttat caagggccgg ctggtgacgg
     4801 gttttgaccg gccggtgata gaagctctgc tgaaggactg aagggaaatg caggaaaggg
     4861 tcgcgaccgt tccctgcatg ttatctgact ttcgcgcccg ttttgggttc gcggtcgaac
     4921 ccgatcagcg tcaatccttc cggagaatcc gtcgccagca gcatgccgtg cgactcgacg
     4981 cccatcaatt tggcgggttt caggttggcc acgatgacga tggatttgcc gaccagctcc
     5041 tggggtttgt aatccttgcc gattcccgcc acaatcgtcc gctcctctcc aatgtcaatt
     5101 cgcagtttga ccagcttgtt ggatttgggc accgcctccg ccgagagaat cttcgccacg
     5161 cgcagatcca ctttggaaaa ttcatcgtag tcgatttccg g
//
LOCUS       DAOSIM010000147         5193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_14263_length_5193_cov_7.005450, whole genome shotgun sequence.
ACCESSION   DAOSIM010000147 DAOSIM010000000
VERSION     DAOSIM010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14263_length_5193_cov_7.005450"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="PLR47_07120"
     CDS             complement(<1..1296)
                     /locus_tag="PLR47_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG12754.1"
                     /translation="MLQNQVWQPLPGSRQAEIYPYLRKPDLLSSNSCIIRTPRQILLI
                     DAGALAAQTADLNRILTDCQRERSRPVVVYLTHCHLDHSLEVGRHRQIMTTAPVWIAI
                     QEQGADYLSEGDSAKTIAELYGVAFPSLRPDIRLLTETDKRRKAPRSISLMQGVGMTI
                     MTEAIHANLEQPMFQQTISIGGGDVLEIYSAPGHSPDSICIRAGEVLFIGDLLAAANP
                     MVAGISGWHREHLIETQKHVQWLLDHKPVRICYPGHGGLIPSGKVRDILQRLQRKTLS
                     LGDVSRMNEERLFQITDYALELLDEAEEVFSSIAGRLLYVAYQLECLEEEEAACRCRS
                     AMPMEKIDACLLEFRKLCRGLDSGKIRRVEFAHGTLAIVEQVKTLFDPRPLAAILPQP
                     LIHRGTSLLLDFIGIANGRRNLEEFIPTDVNAVIDDVLRA"
     gene            complement(1324..3507)
                     /locus_tag="PLR47_07125"
     CDS             complement(1324..3507)
                     /locus_tag="PLR47_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOG12755.1"
                     /translation="MQYTVQPLQQGYANRILSINVATGKMEVRPLDPEVRDFFIGGRG
                     LGLYLLHEQVTSRTKASDPENPLIFSPGPLGGIPQFPGTSKCMAISLSPLTGIPGVSN
                     FGGHFGAYLKYAGFDALTVAGTAREPVMIVIDGFKREISIVGVSEDDQVFHLEKTLVD
                     RFLREGHEKKDLAFLTTGIGAAHTSFGCINSHYYDATKPVPGGKGLFRTKQAGRTGLG
                     SVMSHKRIAAIVVLTAFPRGENPYGAADWNKVKKAGAKLHGIVRDVDPQALKMHLKGS
                     AGLISFMNKDEYQSLPVYNYQRGSHPQADQVCGKIYAQRLFEHRGMDGCFPGCNLRCT
                     KGGWVTLTSGEHRGQRVWVDGPEYETAAGFGSNLGMWHPEFIMEANWHCDNYGIDTIT
                     TAVLMAFIMECGQRGYLTGEDTDGFSLTWGDEQAALQFIHDLAYRRTELARAAARGMV
                     GLTGWIADKYAARTARTKPLEELQAFAMQTKGLPFSLYRTHRSLSMQASYAAASDIGA
                     HHAAAWLVKVDLLGAFPTFESKARALITYPRVRLGNDNLGLCKLPWVDVFNPDSMKQG
                     HADIYINPASQELYADFYNGMLGTNLTWEQIFEQTDRDINLQRVMNVLFFQSATGDRD
                     WIPDRAIGPTEDALYELEKDYNDGQLSVILNLPLPEIQRMPTGEKRAILMRHRKDELR
                     QLILVYYRERGWTDSGVPKVETLEQIGVWRFLTQEARTIIARLNG"
     gene            complement(3652..4053)
                     /locus_tag="PLR47_07130"
     CDS             complement(3652..4053)
                     /locus_tag="PLR47_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12756.1"
                     /translation="MKKILAVIVMILFAVSLVFAAEAVLPKADKNKDGKISHQEYLDA
                     SGAAFDFLDKNRDGVVTKEEMQPNKKTDADRWIREADANQDGKIHKSEHQQAARKRFF
                     SLDKDKSGHIDRNEWASDRSDLYSPFNLFTF"
     gene            complement(4071..4931)
                     /locus_tag="PLR47_07135"
     CDS             complement(4071..4931)
                     /locus_tag="PLR47_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOG12757.1"
                     /translation="MRSTNPYIPMPVEIVKITDEVDTHDIKSFRFKFLNAEDESRFQY
                     LPGQFAELSIYGKGESPIGIASSPTEPGYVEFTVQRAGAIVPGLVTAALHDMEEGGKI
                     GLRGPLGHPWPIEFLEKKNIVVVGGGFAFTTLRSLVTYMLHGDNRPRFGDITVIYGAR
                     TPGLLCYKDELAAWEKRSDIKTIITVDKGNETWKGREGFVPAVCKDVAPSPENAVAVI
                     CGPPVMIKFTLPVFFDLGFSKENILTSLEMRMKCGIGKCGRCNVGYKYVCSDGPVFSL
                     AELEELPRDF"
     gene            complement(4983..>5193)
                     /locus_tag="PLR47_07140"
     CDS             complement(4983..>5193)
                     /locus_tag="PLR47_07140"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG12758.1"
                     /translation="CMFTDFTLEASGHNPRTKVYQRLRQKINHKYSYHVRKYGVISCV
                     GCGRCTRYCPVNIDIFSIVEEAMKA"
BASE COUNT         1072 a         1519 c         1480 g         1122 t
ORIGIN      
        1 cgcccgcagc acatcgtcga tcaccgcgtt gacgtccgtt ggaataaact cctcaagatt
       61 gcggcgtccg ttcgcaatgc cgataaaatc cagaagcagg cttgtgcccc ggtgaatcag
      121 cggctggggc aggatggcgg ccagcgggcg gggatcaaag agcgttttta cctgttcgac
      181 aatcgccagc gtgccatgcg cgaattccac ccgacggatt tttccggaat ccagcccgcg
      241 gcagagtttg cgaaactcca gcaggcaggc gtcaatcttc tccatgggca tcgcgctgcg
      301 gcaccggcag gcggcctctt cctcctccag acactccagt tgataggcca cgtagagaag
      361 gcggccggca atcgacgaaa aaacctcctc ggcttcgtcc aaaagctcca gcgcgtagtc
      421 ggtgatctga aagaggcgct cctcgttcat ccggcttacg tcgcccagcg agagggtttt
      481 gcgctgaagc cgctgcagga tgtcgcggac tttcccggac ggaatcaggc cgccgtgtcc
      541 gggatagcag atcctgaccg gcttgtgatc caaaagccat tgcacgtgct tttgagtttc
      601 gatgagatgc tcccggtgcc agccggaaat tccggcaacc atcggattgg ccgcggcgag
      661 caaatcgccg ataaagagaa cttcgccggc gcggatgcag atgctgtcgg ggctgtgtcc
      721 gggcgccgaa tatatttcca ggacgtcgcc tccaccgatc gagatggtct gctggaacat
      781 cggctgctcc agattcgcgt ggatcgcctc ggtcatgatg gtcatgccca cgccttgcat
      841 cagcgaaatg gaacgggggg cttttcttcg tttgtcggtt tccgtaagca ggcggatatc
      901 cggccgcagg gacggaaaag ccacgccgta gagttccgcg atggtttttg ccgaatctcc
      961 ctcactcaga taatcggccc cctgttcctg gatggcgatc cagacgggag ccgtcgtcat
     1021 gatctgccgg tgcctgccga cctccaggga atggtccaga tgacagtggg tcaggtaaac
     1081 gacgaccggt cgggatcgtt cccgctgaca gtccgtcagg atccggttca ggtcggcggt
     1141 ctgcgcggcc agcgccccgg cgtcgatcaa aagaatctgc cggggtgtgc gaatgatgca
     1201 ggaattggag gagagaagat cgggcttgcg cagatacgga tagatttccg cctgccgact
     1261 tcccggaaga ggctgccaga cctgattctg aagcacggga gactcctttc cagccgtgaa
     1321 tcttcacccg ttcaggcggg cgatgattgt cctggcttcc tgggtcagaa aacgccatac
     1381 gccgatttgc tcaagcgtct ccacctttgg gacgcccgaa tccgtccagc cccgctcgcg
     1441 gtaatagaca agaatcaact gacgcagctc gtcttttcga tgccgcatca gaatggcgcg
     1501 cttttcgccc gtgggcattc gctgaatttc cggcagcggc aaattcagga tcacggaaag
     1561 ttgcccgtcg ttataatctt tctccagttc atagagcgcg tcctccgtgg gcccgatggc
     1621 ccggtcggga atccagtcgc gatccccggt cgcggattga aaaaacagga cattcatcac
     1681 gcgctgcaga ttgatgtccc ggtccgtttg ttcgaagatc tgctcccagg tcagattcgt
     1741 gcccagcatg ccgttgtaga aatccgcata cagttcctgc gacgccggat tgatgtaaat
     1801 gtcggcatgg ccctgcttca tggaatcggg attgaaaaca tccacccagg gcagcttgca
     1861 caggcccaga ttgtcgttgc cgaggcgaac gcggggatag gtgatgagcg ctctggcctt
     1921 ggattcaaac gtgggaaagg cgcccagcag atccactttg accagccagg cggctgcgtg
     1981 gtgcgcgccg atgtcgctgg ccgccgcgta ggaagcctgc atggatagag acctgtgcgt
     2041 gcgatacagg gaaaaaggaa ggcctttggt ctgcatggca aaggcctgca gctcctcaag
     2101 cggcttggtt cttgcggttc gcgccgcgta tttatcggcg atccagccgg tcaggcccac
     2161 catgccccgg gcggcggcgc gggccaattc ggtccggcgg tacgccagat catggatgaa
     2221 ctgcagcgcc gcctgctcat ccccccacgt caggctaaag ccgtccgtgt cttctcccgt
     2281 caggtatccg cgctggccgc attccatgat aaatgccatg agaacggccg tggtaatggt
     2341 atcgatgccg tagttgtcgc agtgccagtt ggcttccatg ataaattcag gatgccacat
     2401 gcccaggttc gagccgaatc ccgcggcggt ttcatactcg gggccgtcca cccagaccct
     2461 ttgtccgcga tgctcgccgg atgtcagcgt gacccagccg cccttggtgc agcgaagatt
     2521 gcagcccgga aagcatccgt ccatcccgcg gtgttcgaac agccgctggg cgtaaatttt
     2581 gccgcagacc tgatccgcct gcgggtgtga gccgcgctgg taattataca caggcaggga
     2641 ctggtactcg tccttgttca taaaagaaat cagaccggct gaacctttca gatgcatttt
     2701 cagcgcctgc ggatcgacgt cccggacaat cccgtgcagt ttcgcccccg cctttttaac
     2761 cttgttccag tcggccgccc cgtaaggatt ttctccgcgg ggaaaagcgg tcagaacgac
     2821 gatggccgcg atgcgcttat ggctcatcac ggaccccaga cccgtgcggc cggcctgttt
     2881 ggtgcggaaa aggcctttgc cgccgggaac cggcttcgtg gcgtcatagt aatggctgtt
     2941 gatgcacccg aaagacgtat gcgccgctcc gatgccggtc gtcaaaaagg ccaggtcctt
     3001 tttctcgtgg ccctcgcgca aaaaccggtc cacaagcgtt ttctcaaggt gaaaaacctg
     3061 atcgtcttcg gagacgccga cgatggagat ttcccgtttg aaaccgtcaa tgacaatcat
     3121 gaccggctca cgggcggttc ccgcgactgt cagcgcgtca aacccggcat atttcagata
     3181 agcgccgaaa tggcctccaa aattggagac accgggaatg ccggtcagcg gcgacaggga
     3241 aatcgccatg cacttgctcg ttccgggaaa ctgcggaatg ccgcccagcg gccccggcga
     3301 gaaaatcagg ggattttccg gatcagaggc tttggtccgg gaagtaacct gctcatgcag
     3361 cagataaagc cccagccccc ttccgccgat aaagaaatca cgaacttcgg gatccagcgg
     3421 acggacctcc attttgccgg tcgctacgtt gatggaaagg attcggttgg cgtatccctg
     3481 ctgaagcggt tgaaccgtat attgcatttg ctgttccttt gaaaatagtc cgcgcacgtt
     3541 acggcaatcc gggaaaaaat tcaaccatca aaagaggttg aaggatggac gatcccccct
     3601 ggggcaaaag cttccccagg gacggtcccg ccgcgtggtg caacacgctt ctcagaacgt
     3661 gaagagattg aaaggggaat agagatcgga gcggtcggag gcccattcat tccgatcgat
     3721 atgaccgctc ttatctttgt ccagggaaaa gaaccttttt cttgcggcct gctggtgttc
     3781 gcttttatgg attttcccgt cctggttggc gtcggcttcc cgaatccatc gatccgcgtc
     3841 ggtcttttta ttgggttgca tctcttcttt cgtcacgacg ccgtcccggt tcttgtccag
     3901 aaagtcaaac gccgcgcctg acgcatccag gtattcctgg tggctgattt tcccgtcttt
     3961 gtttttatcc gccttcggca aaacggcctc cgcggcaaaa accaatgaaa cggcaaaaag
     4021 gatcatcacg atcactgcaa gtattttctt catcattaac ctctttttga ctaaaaatcg
     4081 cgcggcagct cttccagttc cgccagtgaa aacaccggcc cgtccgaaca aacgtatttg
     4141 tagccgacat tgcagcggcc gcatttgccg atgccgcact tcatgcgcat ttccagcgac
     4201 gtcagaatat tctccttgga aaaacccaga tcaaagaaaa ccggcagcgt gaatttaatc
     4261 atgactggcg ggccgcagat gacggccacc gcgttttcgg gggacggcgc gacgtccttg
     4321 cagacggcgg ggacaaatcc ttccctgccc ttccaggttt cattgccctt gtcaacggta
     4381 atgatggtct tgatgtcgct gcgcttttcc caggcggcca gctcatcctt gtagcagagc
     4441 aggcccgggg tccgcgcgcc gtaaatgacg gtgatgtcgc cgaagcgggg gcggttgtcc
     4501 ccgtgcagca tatatgtgac cagcgacctg agcgtggtga aagcgaatcc gccgccgacc
     4561 acaacaatat tctttttctc cagaaactca atcggccagg gatgccccaa cggcccccgc
     4621 aggccgattt tgccgccctc ttccatgtcg tgcagcgccg ccgtcaccag cccgggcacg
     4681 atggcgcccg cccgctggac cgtaaactcg acgtagcccg gctccgtagg cgacgaggcg
     4741 atgccgatcg gagactctcc ttttccgtaa atggacagct ccgcgaactg gccgggcaga
     4801 tactgaaacc gggactcatc ctcggcattc agaaatttaa agcggaacga cttgatgtca
     4861 tgggtatcca cttcgtcggt gattttgaca atctcaaccg gcatggggat atagggattc
     4921 gttgaacgca tattcttcct caaattggtt tattcccgaa tgcgcctttg gggcgttcgg
     4981 cgtcacgcct tcatggcttc ttcgacaatg gaaaaaatat cgatattcac cggacaatac
     5041 cgcgtgcagc ggccgcagcc cacgcaggaa atcacgccgt atttccggac atggtaagaa
     5101 tacttgtggt taattttctg ccgcaggcgc tgatatactt tcgtgcgggg attgtggccg
     5161 ctggcttcca gcgtgaaatc cgtgaacatg cag
//
LOCUS       DAOSIM010000148         5179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_14316_length_5179_cov_6.237900, whole genome shotgun sequence.
ACCESSION   DAOSIM010000148 DAOSIM010000000
VERSION     DAOSIM010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14316_length_5179_cov_6.237900"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLR47_07145"
     CDS             <1..587
                     /locus_tag="PLR47_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG12759.1"
                     /translation="IICFGPQCMIGYHNKPEKTKEVIVEMNGMRGVRTGDRGRLDNDG
                     FLFITGRFKEEYKLANGKYIHPDAVELEMKVLPWILNAMIYGAGQEYNVGLIVPDMKL
                     LKQMAEKLNLSVEPESLFDPGNSAGRNFKALLTTEVQNHLRKTIGSYEIPQKFAFIRE
                     DFTLESGMLTQTMKLKRQAVLDKYGDMLNSLYKE"
     gene            complement(668..1429)
                     /locus_tag="PLR47_07150"
     CDS             complement(668..1429)
                     /locus_tag="PLR47_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12760.1"
                     /translation="MAVSGAIGREDNVIHLTYEDMMMIKKEIISRNPLLNLGFENEYI
                     LQSGGLGAVLAHAGVGKTALLVQMALHAMMNEQPVLHVSLNDAVSKVDIWYRELFHDM
                     ASKNDSAEITEYWENILNYRFIMTFRVEGFSVPKLEERLTDLMQQNIFKPYAVILDGL
                     KFDESGRGLLLELKELAKKYAMRIWFTVHTHRHEPPREDGLPFSFLHVADLFDVLVQL
                     SSEGPEVYIKALKGQSPGAKKDALLLDPATMLIKA"
     gene            complement(1465..3552)
                     /gene="fusA"
                     /locus_tag="PLR47_07155"
     CDS             complement(1465..3552)
                     /gene="fusA"
                     /locus_tag="PLR47_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOG12761.1"
                     /translation="MKFDDQKIRNIGISAHIDSGKTTLSERILYYTKRIHAIHDVKGK
                     DGVGATMDSMDLEKERGITIASAATYCEWKNHEINIIDTPGHVDFTIEVERSLRVLDG
                     AILVLCSVGGVQSQSITVDQQMKRYKVPCIAFINKCDRSGANPFRVIEQLRTKLGHNA
                     VAMQIPIGLETSLEGVVDLAAMKALYFDGDSGEDIRVEDIPPELLAEAAAKREELIDR
                     ASLFSDELTEAILEEKEITPELLYAAVRKGTLKREITPVYMGSAYKNKGVQPLLDGVT
                     YLLPAPSEVENVALDMNHNEEPVVLTSDPEKPIIALAFKLEDGQYGQLTYIRVYEGTV
                     EKGSTIVNVRNGKKVKVGRVVRMHADQMEDVDSLKAGYIGALFGIECSSGDTFVSPGL
                     NMTMTSMYVPKPVISLAIVPKDNKAQMAMSKALNRFSKEDPTFKSHVDHETNETIIEG
                     MGELHLDIYVERMKREYNAEVTTGNPRVAYRETITQRADFNYTHKKQTGGSGQFGRIA
                     GYIEPISDAEFVFENKIFGGAIPTQYIPACEKGFKQSMAKGPKLEFPITGVKVVINDG
                     AFHAVDSSDMAFQAAARGAFKEAYLRAKPVVHEPIMKVVVETPTEFQGSVMGLLNQRR
                     GMIIGSQDEGVMCVIEAQVPLGEMFGFSTILRSSTQGKAQFTMEFALYRQVPQSIAEK
                     IALEVAQNKKTAA"
     gene            4444..>5179
                     /locus_tag="PLR47_07160"
     CDS             4444..>5179
                     /locus_tag="PLR47_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12762.1"
                     /translation="MQEETQSKGARDESEAPLRGHFLSQAISGDAGQPFKVTINDITF
                     PAYLLNNNYEIDWINKDAESSIFQEEIHTIKEPTSRNIFQLLLRSRHENQQARSALID
                     FHLSFVKGKAVKPELQHLYDGMSPRERENLEKRYNCIESPPRQAVNKIPLKLSGQKGT
                     PIANDVYYMVFREGTLFIHAPKACQNQGIADLFASRSSVINDLLKQRMPTLVSFCVLV
                     ADLQNSVRICAELPPEEYFELINQVWA"
BASE COUNT         1245 a         1373 c         1277 g         1284 t
ORIGIN      
        1 aaatcatctg cttcggcccc cagtgcatga tcggctacca caacaaaccg gaaaaaacaa
       61 aagaagtgat cgtggaaatg aatggaatgc gcggcgtgcg caccggagac cggggacgcc
      121 tcgacaacga cgggtttctg ttcattacgg gccgcttcaa ggaagaatac aagctggcca
      181 acggaaaata catccacccg gacgccgtcg aactggaaat gaaggttctg ccctggatcc
      241 tgaacgccat gatttacggg gcgggccagg aatacaacgt gggcctgatt gttcccgaca
      301 tgaaactgct caagcagatg gccgagaagc tgaatctgtc cgtggaaccg gagagcctgt
      361 tcgatcccgg caattccgcc ggccgcaatt tcaaggcatt gctgaccacc gaggtgcaga
      421 atcatctgag aaaaacgatc ggctcttatg aaattcccca gaaattcgct tttatccggg
      481 aggatttcac gctggaaagc ggtatgctca cgcagaccat gaagctcaag cgtcaggcgg
      541 tcctggacaa atacggggat atgctgaaca gcctgtacaa agagtaaaac ggagcttccc
      601 gcaccggatg gaaacgcggg agggatcacg aaaaaacgga gacgggccgt ccgcctgtct
      661 ccgtttttta tgctttgatg agcatggtcg cgggatccag aagcagcgca tcctttttgg
      721 cgccgggcga ctggcccttg agggctttga tatagacttc gggcccctcg gaagacagct
      781 gcaccagcac atcaaaaagg tcagccacat gcagaaaaga aaacggcaga ccgtcttccc
      841 tgggcggctc atgacggtgc gtatgaacgg taaaccagat gcgcatggca tattttttgg
      901 ccaattcttt cagctcgaga agaagaccgc gtccggattc atcaaatttc agcccgtcca
      961 gaatgacggc atacggcttg aaaatattct gctgcatcag gtcggtcaga cgttcttcca
     1021 gcttaggaac gctgaatcct tccacacgaa aggtcatgat aaagcgatag ttgaggatat
     1081 tctcccaata ctccgttatt tcagcagaat cattctttga cgccatgtcg tgaaataact
     1141 cgcgatacca gatgtccacc ttgctgacgg cgtcgtttaa gctgacatgc aaaacaggct
     1201 gttcgttcat catggcgtga agagccatct gcaccaagag ggctgttttg ccgacgccgg
     1261 catgagccaa aaccgcgccc aaaccgccgc tttgtaaaat atattcgttt tcgaacccca
     1321 ggtttaaaag cggattccgt gaaataattt cttttttgat catcatcatg tcctcatatg
     1381 ttaaatggat gacattgtct tcccgtccga tcgctcctga aactgccatc tttttttact
     1441 tcatccttca gtcttcagcc tgtcttacgc cgccgtcttc ttgttttgag cgacttcgag
     1501 ggctattttt tccgcgatcg actgcggcac ctggcgataa agagcaaact ccatcgtgaa
     1561 ttgcgccttg ccctgcgtgg aggaacgaag gatggtcgaa aaaccgaaca tttccccaag
     1621 cggcacctgt gcttcaatca cgcacatgac gccttcgtcc tgtgatccga taatcatccc
     1681 gcgccgctga ttgagcagcc ccattaccga tccctgaaac tccgtgggtg tctccacaac
     1741 aactttcatg atcggttcat gaacaaccgg cttggcgcgc aggtaggctt ctttgaaagc
     1801 gccgcgggcc gccgcctgaa acgccatgtc cgaagaatcc accgcgtgga atgcgccgtc
     1861 attgatgacg actttcacgc cggtgatcgg aaactccagc ttcggccctt tggccatgct
     1921 ctgtttaaat cctttttcac aggcgggaat gtactgcgtg ggaatggcgc cgccgaatat
     1981 tttgttttca aagacaaatt cggcgtcgct tatcggttcg atgtagccgg caatgcggcc
     2041 gaactgcccg gagccgccag tttgcttttt atgcgtgtag ttgaagtccg cccgctgggt
     2101 aatggtttcc cggtaagcca cacgcggatt gccggtggtc acttccgcat tgtattcacg
     2161 cttcatgcgc tcgacgtaaa tatccagatg cagttccccc atgccctcga tgatggtttc
     2221 attggtttca tgatcaacat gggatttgaa tgtcggatct tctttggaaa aacggttcaa
     2281 cgccttggac atggccatct gagccttgtt gtctttgggc acgatggcga gcgagatgac
     2341 cggtttgggc acatacatgg acgtcatggt catgttcagt ccgggcgaaa cgaacgtgtc
     2401 gccggaagag cattcgatcc cgaacaaggc gccgatgtat ccggccttga gcgaatccac
     2461 atcctccatc tgatcggcgt gcatgcgcac gacgcggccg actttgactt tttttccgtt
     2521 tcgcacattg acgatcgtgg atcctttttc caccgtccct tcgtaaacgc gaatataggt
     2581 caactgcccg tactggccgt cttccagttt gaacgccagc gcgataatcg gtttttccgg
     2641 atcgctcgtc aggacaaccg gctcttcgtt atgattcatg tccagggcga cattttccac
     2701 ctccgacggc gcgggcagca gatacgtcac gccgtccagc agcggctgaa cgcctttgtt
     2761 cttatacgct gatcccatat aaacgggtgt gatttctctt tttaacgttc ccttgcggac
     2821 ggccgcgtaa agcagttccg gcgtgatttc cttttcctcc agaatcgctt ccgtcagctc
     2881 atcggaaaaa agggacgccc tgtcaatcag ctcttctctt ttggccgcgg cttccgccag
     2941 aagctccgga gggatgtctt ccacgcgaat atcctcgccg ctgtccccgt cgaaatacaa
     3001 ggctttcatg gccgccagat cgaccacacc ttccaggctg gtttccaatc cgatgggaat
     3061 ctgcatggct acggcgttat gccccagctt ggtccgaagc tgttcaatga cccggaaggg
     3121 attggcgccg ctgcggtcgc atttgttgat aaacgcaatg caggggactt tgtagcgctt
     3181 catctgctga tcgaccgtga tggactgcga ctgaacgccg ccgacggagc acaaaacaag
     3241 gatcgcgccg tccagaacac gcagggatcg ctctacttct atcgtgaaat ccacgtggcc
     3301 tggagtatca atgatattga tttcatggtt tttccattcg caataggtcg ccgcggaagc
     3361 gatggtgatg cctctttcct tttccaggtc catggaatcc atggtcgcgc ccacgccgtc
     3421 tttgcccttc acgtcgtgga tggcgtgaat tcttttggta taataaagaa tgcgctcgct
     3481 gagcgtggtt ttaccggaat caatgtgcgc gctgatcccg atgttgcgta ttttctgatc
     3541 gtcgaacttc atgatgcttt cctctcactg actgaatata aaaaaccccc gcatttggaa
     3601 gagtctctgc cagatggggg atccaccgta atcgagcctt agaaataagg gttggacgtt
     3661 tatatcctta atccggattt gtcaagctaa tgttttcatt aatttccagg agaactttca
     3721 gcgcatcgct ttgcgtggcc cggttaaacg cctcctgcac ttgtttcagc ggataccttg
     3781 cggtgacgat tctgtcgagg ggcttgccgg gatacttccg catgatgttc aggccgtcgg
     3841 caaaccggcc gccgccacgc tttccatata ccttcttttc aatggtttct gaaattttcg
     3901 atccttttct tataaacggg agaacatata gaaaacggga tattgtaagt caaggagttc
     3961 ttgcctggcg ttcttgcctg gtgattttta tttgtcaata attggacagt ctaataaatt
     4021 gtcttagact gtataattat acttgacaac tgtgtttttg aactgtataa gttagccgca
     4081 acaggcacaa atgtcctttt taggaagaat gtcataacca tgccactcta gggggtcaaa
     4141 atgaatgatg acgcaccgat aaggaatcaa tggattagca gcctggaggt catcaataaa
     4201 acaggaattt ccagggcaac gcttaacaac tacattaaga tgaaccttct gccccgtcct
     4261 gccgtcaaaa ggccggaaac aacagatgcc cgcgcccgaa tgctcggcta ttttcctgac
     4321 tccgtgattg atacaattat acagatcaaa gaattaaaaa agcagggttt ctccatggac
     4381 gccatcacgg agaggttgaa acaatctcgc ttaaccccgg ctattgattc aggagtcatg
     4441 aatatgcagg aagaaaccca aagcaaaggg gcccgggatg aatcagaagc ccctctccgg
     4501 ggacactttc tttctcaagc gatctccggg gacgccggcc agccattcaa ggtgaccatc
     4561 aacgatatca ccttccctgc ttacctgctg aataataatt acgagattga ctggattaac
     4621 aaggatgcgg agagttccat ttttcaggaa gaaattcaca ccatcaaaga gccgacgtca
     4681 agaaacatct ttcagctcct cttgcgcagc cgacatgaaa accaacaggc ccgaagcgcg
     4741 ctcatcgact ttcatctcag ttttgtaaag ggcaaagccg tcaaacccga actgcagcat
     4801 ttgtatgacg gcatgtcgcc cagggaaaga gaaaatcttg aaaaaagata taattgcatc
     4861 gagtccccgc cccggcaggc cgtcaacaaa atccccctga agctatccgg gcaaaaagga
     4921 acgccgattg ccaatgatgt ctattatatg gtttttcgcg aaggaacact ctttattcac
     4981 gcgccgaaag cctgtcagaa tcagggaatc gctgatttgt ttgcctcccg atccagtgtg
     5041 attaatgacc tcctcaaaca gcgaatgccc acgctggttt ccttttgcgt gctcgtggcc
     5101 gacctgcaga actcggtgcg catttgcgcc gaattgcctc cggaggaata ttttgaactc
     5161 atcaaccagg tatgggcct
//
LOCUS       DAOSIM010000149         5143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_14455_length_5143_cov_7.353577, whole genome shotgun sequence.
ACCESSION   DAOSIM010000149 DAOSIM010000000
VERSION     DAOSIM010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14455_length_5143_cov_7.353577"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLR47_07165"
     CDS             complement(<1..217)
                     /locus_tag="PLR47_07165"
                     /inference="COORDINATES: protein motif:HMM:NF026110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12763.1"
                     /translation="MADRTIHITQFDMDRLTELIDGLRLSPKANQANLDLLEKELYRA
                     VLVDPPNIPRDVITMNSKVIITDTESGE"
     gene            561..>1304
                     /gene="htpX"
                     /locus_tag="PLR47_07170"
     CDS             561..>1304
                     /gene="htpX"
                     /locus_tag="PLR47_07170"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF003965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HOG12764.1"
                     /translation="MKRVLLFIGTNIAVLVVLSIVIKLLGVDQILYANGINYLNLLIF
                     SAVFGFGGSFISLAMSKWMAKWSTGAQVIMQPRSDAEIWLVNTVQKQAAQAGIGMPDV
                     AVFESPSPNAFATGMNKNNALVAVSTGLLRSMNRNEVEAVLGHEISHVANGDMVTLSL
                     IQGVVNTFVIFLSRIVGYFVDRVVLKNEEGHGIGFFITTIVAQIVFGILASVIVMWFS
                     RRREYRADAGGARLAGVGSMIAALESLQRS"
     assembly_gap    1305..1314
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1475..2422)
                     /locus_tag="PLR47_07175"
     CDS             complement(1475..2422)
                     /locus_tag="PLR47_07175"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HOG12765.1"
                     /translation="MEKIRLGISTCLLGENVRYDGGHKLDRFLRDTLGRYVDYVPVCP
                     ETECGMGIPREAMRLEGRPDAPRLVTRTSRIDKTDMMVSWANMRIAKLASEELCGFIF
                     KSDSPSSGMERVKVYDEKGMPAKTGVGIFARMFMDAFPLLPVEEEGRLHDPQLRENFI
                     ERIFALRRWREVRRARSPRGALVDFHTRHKLLLLSHSTKLYQAMGKLVAEQKRHALQD
                     LWAKYEALLMEALKLKTTPKKNVNVLMHMMGYFREKLSADEKQELLEIFERCAQGHVP
                     LIVPLTLIGHHVRKYDEPYLKEQVYLNPHPLELQLRNHV"
     gene            2566..3783
                     /locus_tag="PLR47_07180"
     CDS             2566..3783
                     /locus_tag="PLR47_07180"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12766.1"
                     /translation="MLNHDLSFFYYIVLFAAYCFIGWVAEVMYRSAAQRKFVNAGLLF
                     GPFIPIYGIGAFLVVVLEYALQGRPIALRLAAFGLVLTLLEYAAGYLSEKIFKLKLWD
                     YSEYRFHLHGRVSLLFSFFWTVLAFGFVTMIHPEALRLSALLPAGPVQIAAIVFMVYF
                     WIDYTFSVMSLAAFRKGVAYLYEEYFNLSSLEIEDILKSFQRLREAFPDLNRYVNQNI
                     NRRIRIKIGSFLKPVLEKFFQELDGRKPFEAEYYETVREILDHEEFRKLKDYFHHNSS
                     IYRHVHDVAYLSYRISKFLKLDFRSTARGALLHDFFLYDWRHHDAPDLPREKFHGLEH
                     PKIAAANAKKYFSLNDIEEDIIRKHMWPLTIVPPKYKESYVVSFADKYLSSREFISEY
                     KKRIEKRRSKASE"
     gene            complement(3827..4447)
                     /locus_tag="PLR47_07185"
     CDS             complement(3827..4447)
                     /locus_tag="PLR47_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12767.1"
                     /translation="MPNSAILLKNIVIAPPAAQIYSRLGFKRATTSLSSGRRRETDRH
                     MEEAASMIRLQGSFLRLSIRRNDGRLISLQGRTTFRSEKLSAFLGDCRDAVLMGATAG
                     KPIMDAIAEETARGNLTAAVVYDATASEMTDAALDWIMNYVNGQLRREGRRLLPRRFS
                     AGYADFSLSSQKVIHKKLRMEEIGVSITPACILQPEKSVTAIGAIP"
     gene            complement(4460..4864)
                     /locus_tag="PLR47_07190"
     CDS             complement(4460..4864)
                     /locus_tag="PLR47_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG12768.1"
                     /translation="MKGKILIVDDEESMRRLLSQLFSAEGYEVAAAENGPSALEILRH
                     SVIDVIFLDLKLFSMDGIELCRKIRETQPVSMIFAVTGWSPLFGIEECRGAGFDDYFE
                     KPLDMKLILASVEDAFGKLNRWKRLYSNPSTP"
     gene            complement(4861..>5143)
                     /locus_tag="PLR47_07195"
     CDS             complement(4861..>5143)
                     /locus_tag="PLR47_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOG12769.1"
                     /translation="IHGSAPDIAGKNIANPLATILSVALMLRHSLDRPEEAALVEQSV
                     KSVLREGLRTPDIAAAGGGAATTEEMGEAVVRNLSSLLRGTAGKEVKSP"
BASE COUNT         1045 a         1437 c         1455 g         1196 t
ORIGIN      
        1 tttcgccgga ttccgtgtcc gtgatgatca ctttggaatt catggtaatg acatcccggg
       61 ggatattcgg aggatccacc agcacggcgc gatacagctc tttttccagc agatcgagat
      121 tggcctgatt ggctttgggc gataaacgca aaccgtcgat cagttccgtc agcctgtcca
      181 tgtcgaattg cgtaatgtgt attgtgcggt ctgccatgat gctgtgtcct ccttttggcc
      241 tggtctgggg gttgaaaacg gttgccggat ccaaaagccg caaaaacggc gattattgtt
      301 tgataaaaaa cggaacaccg tcctggccga tgctccgaat tgcaaagcac cctatcacag
      361 ttttcaaaaa aaaagaacag gcaatccgga tcttttccgc tcaaatcccc gcagcggcat
      421 tccggcggag gatttgcggg ggtgagggcc gccggagaag aaacgcttga cattccgtga
      481 cctgacatta agataaggca tcattttaaa aggtttgcca ggcaaacagt ttgcgcgatt
      541 ttaaaccgga ggagatcaaa atgaaaagag ttttgctgtt tatcgggacc aacatcgccg
      601 tgctcgtggt cttaagcatt gtcataaagc ttctcggcgt ggatcaaatt ctttacgcca
      661 atggcatcaa ttacttaaac ctgctgattt tttcggcggt tttcggcttc ggaggatcgt
      721 tcatatcgct ggccatgtcc aagtggatgg ccaagtggag caccggcgcc caggtcatca
      781 tgcagccccg ctccgacgcg gaaatctggc tggtgaacac ggttcagaag caggctgctc
      841 aggcgggcat cggcatgccg gatgtagcgg tttttgaatc gccgtcgccc aacgcctttg
      901 ctaccggcat gaataagaac aacgcgctgg tggcggtcag cacggggctt ctgcgcagca
      961 tgaaccgcaa tgaagtcgag gcggtgctgg gccacgagat cagccacgtg gccaacggcg
     1021 acatggtgac gctcagtttg attcagggcg tcgtcaacac ctttgtcatt tttctgtccc
     1081 gtatcgtcgg atattttgtg gatcgtgttg ttctgaaaaa cgaagaaggc cacggaatcg
     1141 gattttttat caccacaatc gtcgcgcaga ttgtcttcgg catcctggcc agcgtcattg
     1201 tcatgtggtt cagccgccgg cgggaatatc gcgcggatgc cggcggcgca aggcttgcgg
     1261 gcgtgggcag catgatcgcc gcgctggaga gcctgcagcg cagcnnnnnn nnnncctgcc
     1321 ggatcagatg caggccttcg gcatcagcgg aaagcccggc aaaaacagtc taaagcttct
     1381 gtttatgacg catcccccgc tggaagaccg catcgaagcg ctgaagcgct ccgccggaca
     1441 ggcggcgtaa caaagattcg cgcgggcggc cggtttatac gtggttgcgc agttgaagct
     1501 ccagcgggtg aggattcaga tacacctgct ccttcagata gggttcatcg tatttgcgca
     1561 catggtggcc gatcagcgtg agaggtacga tcaggggaac atgaccctgc gcgcagcgct
     1621 caaagatttc cagaagctcc tgtttttcat cggcggagag tttttcccga aagtagccca
     1681 tcatgtgcat cagcacattg acattctttt tcggcgtggt tttgagtttc agcgcttcca
     1741 tgagcagcgc ttcgtacttt gcccaaagat cctgcagggc gtggcgcttt tgttcggcga
     1801 ccagttttcc catggcctga tacagttttg tgctgtgcga cagcaaaagc agcttgtgcc
     1861 gtgtgtgaaa atccaccagg gcgccccggg gggacctggc cctgcggact tcgcgccagc
     1921 gcctgagcgc aaaaattctt tcaatgaagt tttcgcgaag ctgcggatca tgcagccgcc
     1981 cttcctcttc caccgggagc aggggaaaag cgtccatgaa catccgcgcg aagatgccca
     2041 cgccggtttt ggcgggcatt cctttctcat catagacctt gactctttcc atgccgctgg
     2101 acggcgaatc gctcttgaaa atgaagccgc agagctcctc ggacgcgagc tttgcgatgc
     2161 gcatattcgc ccaggaaacc atcatgtccg ttttgtcgat gcggctggtg cgcgtgacga
     2221 gccggggcgc gtccggtctg ccttccaggc gcatggcttc gcggggaatg cccatgccgc
     2281 actcggtctc cgggcaaacc ggcacataat ccacataccg ccccagcgtg tctcgcagga
     2341 aacggtcaag tttgtgtccg ccgtcatagc ggacgttctc tcccagaaga caggtgctga
     2401 tgccgagtct gattttttcc atggcgtctc cttcccgctt ccgtccatgg acggcaggcg
     2461 gtgtgttcaa aggctccttc tcataccaca gatggatgca aaatatcttt ataaaaagag
     2521 gggaattgtg tagcatcggc ctgcataaaa aggccgggga aaaccatgct gaaccatgat
     2581 ctgagcttct tctattatat tgttctgttt gcggcgtact gctttatcgg atgggtggcg
     2641 gaggtgatgt accgctccgc ggctcagcgg aagttcgtca atgcgggatt attgttcggc
     2701 cccttcattc ccatttacgg catcggcgcc tttctcgtcg ttgtcctgga gtacgctctg
     2761 caggggcggc ccatcgcgct tcgtctggct gccttcggcc tggtgctaac gcttcttgaa
     2821 tacgcggccg gctatctgtc cgaaaagatc ttcaaactga agctttggga ctactcggaa
     2881 taccgcttcc atctccacgg ccgcgtgagc cttctttttt cctttttctg gaccgtcctg
     2941 gccttcgggt ttgtgacgat gatccatccc gaagctctcc ggctttcggc cctgctgccc
     3001 gcgggccccg tgcagatcgc cgccatcgtc ttcatggtgt atttctggat cgactacact
     3061 ttctccgtga tgtcgctggc cgccttccgc aagggcgtcg cctatctcta cgaggagtac
     3121 ttcaacttaa gcagcctcga aatcgaggat attttgaaat cctttcagcg gctgcgggaa
     3181 gcttttcccg acctgaaccg gtacgtcaac cagaacatca accggcgcat ccggatcaag
     3241 atcggttcgt ttctaaagcc cgttttggaa aaattcttcc aggagcttga cggccggaag
     3301 ccgtttgaag cggagtatta cgagaccgtg cgggagattc tcgatcatga ggaattccgg
     3361 aagctcaagg actacttcca tcacaactcc tccatttatc gccatgttca cgacgtggcc
     3421 tacctgtcct accggatcag caaatttctc aagctcgatt tccgctccac ggcccgtggc
     3481 gcgctgctgc acgacttttt tctctatgac tggcgccatc acgacgcgcc ggatttgccc
     3541 agggaaaaat tccacggcct ggaacatccg aaaatcgccg cggccaacgc caaaaaatat
     3601 ttttctctca acgacattga agaggacatc atccgcaagc acatgtggcc gctcaccatc
     3661 gtgccgccga aatacaagga gtcctacgtc gtttcatttg cggacaagta tctgtcttcc
     3721 agggaattca tcagcgagta caaaaaaaga atcgagaagc gccggtcgaa ggcgtccgaa
     3781 tgaacagcgc atttttgcga tggggccgcg ggcggcttgg tttttttcag gggatggcgc
     3841 cgattgcggt aacggacttt tccggctgaa gaatgcacgc cggcgtgatg gagacgccga
     3901 tttcttccat tcgcagtttt ttgtgaatga ctttttggct ggacagggaa aagtccgcgt
     3961 agcccgcgga aaagcggcgc ggcagcaggc gcctgccttc gcgccggagc tgaccgttca
     4021 cataattcat gatccagtcg agcgccgcgt cggtcatttc actggcggtt gcgtcgtaaa
     4081 cgacggcggc ggtcaggttt ccccgggcgg tttcttccgc gatggcgtcc atgatcggtt
     4141 tgccggcagt cgcccccatc agcaccgcat cccggcagtc gcccagaaac gcggaaagct
     4201 tttcgctgcg gaaggtcgtc cgtccttgaa gggagatgag ccgcccgtca ttccggcgga
     4261 tggaaagccg caggaaggag ccctgcagtc gaatcatcga ggccgcttct tccatgtgcc
     4321 ggtccgtttc ccgccggcgg ccggatgaaa gggacgtggt cgctctttta aatccaagcc
     4381 gggaatagat ctgcgccgcg ggcggggcga tcacaatgtt tttcagcaaa atggcggagt
     4441 tgggcatgag tcgtccggct tatggagtgg aggggttcga atacagtctt ttccaccggt
     4501 tcagctttcc aaaggcgtct tcgacggagg ccaggatcag cttcatatcc agcggctttt
     4561 caaaataatc gtcaaagccg gcccctcggc attcctcgat gccgaacagc ggagaccatc
     4621 cggtgacggc aaaaatcatg gagacgggct gtgtttcgcg gatcttccgg cagagctcga
     4681 tgccgtccat gctgaagagc ttcagatcca gaaaaatgac gtcgatgacg ctgtgccgaa
     4741 gaatctccag cgccgaagga ccgttttccg cggcagcgac ctcgtagcct tccgcggaaa
     4801 agagctggga aagcagtctc cgcatggatt cctcgtcgtc cacgatcaga attttgcctt
     4861 tcatggcgat ttgacctcct ttccggccgt cccccgcagg agggaggaaa gattccggac
     4921 gacggcttcg cccatctctt cggttgtggc cgccccgccg cccgccgcgg cgatgtccgg
     4981 tgtgcgcagg ccctcccgca gaacgctttt caccgattgt tcgaccagcg ccgcctcttc
     5041 ggggcggtcc agtgaatgcc gcagcatcag ggcgaccgac agaatcgtcg ccagcgggtt
     5101 ggcgatgttc tttcccgcga tgtccggcgc ggagccgtgg atc
//
LOCUS       DAOSIM010000150         5082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_14682_length_5082_cov_6.476825, whole genome shotgun sequence.
ACCESSION   DAOSIM010000150 DAOSIM010000000
VERSION     DAOSIM010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14682_length_5082_cov_6.476825"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLR47_07200"
     CDS             <1..298
                     /locus_tag="PLR47_07200"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOG12770.1"
                     /translation="MGDKVLRHFSDMARSSFRKSDILARYGGDEFVMLLTNTSLEAAE
                     SATQRLQREFGAWAKNNGLQTGVSFGLAAISKGKNNLDDILRKADAALYEAKPK"
     gene            628..4911
                     /locus_tag="PLR47_07205"
     CDS             628..4911
                     /locus_tag="PLR47_07205"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG12771.1"
                     /translation="MNMKKRIAAGIVFGWFTLFAATGFCQDDGRIGMDQWTRSVSSKN
                     LPTPAPGGFVPAMNRGNELAKAGRYKEAMDAFSEAVRLSPQSSEAYVGRGNAKRAAKD
                     FAGAMNDYEAAVNLKPGDKDLLITLANIKTDMRKYDEAIADLDRILAADPNNLDAIVS
                     RGYARKQLKNYSGAKADFEKAMTLSPNHGFASRMLQSLGSSGQTTSPQQTQIKPLPTL
                     KPTDASHPQAGPSPVTVALPPVAGRVPAPAVSGQDGKPAVPKPFPAASQAAAPGGVLQ
                     EFDPCKPDVRISDLVWSSVEQPAQKTQKQAKSDSRFNDLLVFSSTRNNHPAAVAKAQM
                     AYLMGDLTEADQAKFDEKWSYFFRYPSDELTAYFEKLIPVLADLIEKRNMTTSAVLAR
                     DEAMKDAEEAQTYENTEALTNALMIARQQKDILRELDGELKALAAGIEALGDPPNPLA
                     NACQSRKKHEEARKVAKDALKASIVPKKLKGETGVSYAFKPELSGVEAGAKLHWDFGD
                     GEVKEVPAGEVKHIFKKPGRFVVTLSLPRKEKNEKILLADSRVFMTGVEIQQAGAAVP
                     ETKKVDFPYQDSFSVPAIPPGCSIKWNQGQKANDAVHEDTRLKNPEKYPYYYLFNFRM
                     RCPGTTVLEKGELNIPYKKTPLPYKIDYYYSGDGSPFVTMTLKYMPDHTQSQLNEYLN
                     FPSYEKAAQWKPFKFPGWEANGYIKEDQRHRGFQFIGLGENRTILEINYNSPMVQFKR
                     ESNILEQCMNGDRRCNKEESKISDDYDRDERARRVKVHTDALHKKAIEFISGIKLKGL
                     STHYNAAKSPLAMTAVDEQWLNEEPEAVTADDFKKRQERLAEAEKFYRSNIEMLQKNL
                     IREQADYAREQDPDRKAAFFFRMMGIQADIQAEQDKINTLHTGKIVHTRTQYDDYVQN
                     KFIERIKEEQEKYVKLERAAAIIRKETKKLPPGEREKAAEFVDKHINGKTLANLDEET
                     VKKAGEAIFNKTQGYNEAEAAKYEEEAYMWDDYLTRAERVKMAADIGMVGCSFLGGPM
                     ALSSAYDISTGYFQGGIKEAATQLVLQTGAFALGKYVGAGSKAATGADDAAAAAKTAA
                     RQSGKVSKGLSSAEELKIFQANRARGEKTVKEFENLNQSLKNAYLNKASQNEIAAIKK
                     QIREKTVEIQANPHAKNFLKYQGDKLTQTRFNRSIKSVHQDVEKKFYEKMRKEPYQWS
                     EFKIKEMRNASSAGSVGMDYDIGLIEKPNFIKLSDGTMKRQYWLMRDGKPATRSQWQQ
                     EAQKCWNEAYEEVALKNAERSWENLTTRIHPESYQDLAVLDGNLNQVSKAWIQQTADV
                     TRYKAMHMLKDGSMSKFEKLQEISRGTAKDLNTKLMPIFESMQKTKQVSAAAEHWKKV
                     SSVLDDFGKNKIDPITAERRIAELTGGKSIPEVVDDMSYMMESFVKLGK"
     gene            complement(4895..>5082)
                     /locus_tag="PLR47_07210"
     CDS             complement(4895..>5082)
                     /locus_tag="PLR47_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12772.1"
                     /translation="SPRMAYLIDIRLFLFAWRSDYIFIIYLSTTNFSPLKNFRSKAAE
                     SGLPVDIIKLNGITSPV"
BASE COUNT         1478 a         1236 c         1349 g         1019 t
ORIGIN      
        1 aatgggcgac aaggttctcc ggcatttttc cgacatggcg cggtcaagct tcagaaagtc
       61 cgatatcctt gcgagatacg gcggagacga gtttgtcatg cttcttacaa acacatctct
      121 ggaggccgcg gagtcggcaa ctcaaagact tcagcgcgag ttcggcgcct gggccaaaaa
      181 caacggatta cagaccggcg tcagtttcgg acttgccgcg atatccaaag gcaaaaataa
      241 tcttgatgat atcctgcgca aagcggatgc agcgctatat gaggcaaaac cgaaataggg
      301 gtcaaatctt tattcttgac atttcatctg ccgagcaatg tgttctgcaa tgttgtcatg
      361 aatcgacttg gcataaaggg tcagaagccg gatgcccagt aaaaccagca atgccgtccc
      421 agcaaaaccc tcttgacacg taaactctcg tcttttatac tcaaatccga aaaatgatat
      481 ggtttccagt ctcttcaatt tcaatggcat gaaggaatcc tatctgcttt cttaatggat
      541 gttgcgtcaa cgctattggg gcgtaattat gcaatgccat tgtaatccta acctttaaag
      601 ccaaagccag actacgggag tcgaaaaatg aatatgaaaa aacgaattgc cgcgggcatc
      661 gttttcggat ggtttactct ttttgccgcg acgggttttt gccaggatga tggcaggatc
      721 gggatggatc agtggaccag atccgtaagt tcaaaaaatt tgccgacgcc agcgccgggc
      781 ggttttgttc ccgccatgaa ccggggcaat gagcttgcga aagccggtcg ttacaaggag
      841 gcgatggacg ctttttcgga agctgtgcgt ttaagcccgc aaagcagcga ggcctacgtc
      901 ggcagaggca atgcaaaacg tgccgcaaaa gatttcgccg gagcaatgaa cgattacgag
      961 gccgcggtca atctgaaacc gggcgataag gatcttctga tcacgctggc caatattaaa
     1021 acggacatgc gaaagtacga cgaggcgatt gccgatttgg acaggattct ggctgccgac
     1081 ccaaataatc ttgacgccat cgtatcgagg ggatacgccc gcaaacaact caaaaactat
     1141 tcaggagcaa aagccgattt tgaaaaagcg atgaccctca gcccgaatca cggtttcgca
     1201 tcacgcatgc tccaatcgct gggatcatcc ggacagacga catcccccca gcaaacgcag
     1261 ataaaaccgc ttccgactct gaagccgacc gacgcgtcgc atccgcaggc aggcccatct
     1321 cccgtaacgg ttgctcttcc gcctgttgca ggccgggtcc ccgctcccgc agtttccgga
     1381 caggacggca agcccgctgt cccaaagccc ttccctgccg catcgcaggc ggctgccccg
     1441 ggcggcgttc ttcaggaatt tgatccctgc aagccggatg tccgcatcag cgatctggtg
     1501 tggtcaagcg ttgagcagcc ggcgcaaaag acgcaaaagc aagccaaaag cgattcccgc
     1561 tttaacgatc ttctcgtatt ttcatcgacg cggaacaatc atcccgcggc cgtcgccaag
     1621 gcgcaaatgg cttatctgat gggagacctg actgaggccg accaggcaaa gttcgatgaa
     1681 aaatggtcgt attttttccg ctatcccagc gatgaattaa cggcttattt tgaaaaactg
     1741 attccagtgc tggctgattt gattgaaaaa agaaacatga cgacgtccgc cgttctggcc
     1801 agagacgaag ccatgaaaga tgccgaggaa gcgcagacgt atgaaaatac ggaagcattg
     1861 accaatgcct tgatgattgc ccggcagcaa aaagacattc tgcgtgagtt ggatggagag
     1921 ttaaaggcgc tggcggcagg catcgaagcg ctgggtgatc cgcccaatcc gctggccaat
     1981 gcctgccagt ccagaaagaa gcatgaagaa gccaggaagg tcgccaaaga tgcgctgaaa
     2041 gcatccattg taccaaagaa gctcaagggt gaaacgggtg tatcttatgc gttcaagccg
     2101 gaactctcag gggttgaagc cggcgcgaaa ctgcactggg atttcggcga cggagaagtc
     2161 aaggaagtcc cggcgggaga agtgaagcat atcttcaaga aacctggacg tttcgtggtc
     2221 actctgtcgc tgccacgcaa agaaaagaac gaaaaaattc ttctggccga ctcacgggtt
     2281 ttcatgacgg gcgtcgagat tcagcaggca ggcgcagccg tgccagagac aaaaaaagtg
     2341 gatttcccct atcaggattc cttttccgtt ccggcaatcc ctccaggatg cagcataaaa
     2401 tggaatcagg gtcaaaaagc caacgacgcc gttcatgaag ataccaggct gaaaaatcct
     2461 gaaaagtatc catactatta cctctttaac ttccgaatgc gctgcccggg gacaacggtt
     2521 ctggagaaag gagaattaaa cattccgtat aaaaaaacgc cgcttcctta taaaatcgac
     2581 tattactact cgggagacgg cagccctttc gtaacgatga cacttaaata catgccggat
     2641 catacgcagt cacagctcaa tgagtatttg aattttccca gctatgaaaa ggccgcacag
     2701 tggaagccct tcaaatttcc cggatgggag gccaacggtt atattaagga agatcagcgg
     2761 catcgtggct ttcaattcat cgggctgggt gaaaaccgaa cgattcttga aattaattat
     2821 aacagtccga tggtgcagtt caaacgggaa agcaatattc tggaacagtg catgaacggc
     2881 gaccgtaggt gcaacaagga agaaagtaaa atttccgatg actatgatcg ggacgaaaga
     2941 gccaggcgcg tcaaggtgca tacggatgcc ttgcacaaga aagccattga atttatctcc
     3001 ggcatcaaac tcaaggggct gtccactcat tacaatgccg ccaaaagccc gttggcgatg
     3061 actgctgtcg atgaacaatg gctcaatgaa gaaccggaag cggtgacggc cgacgatttc
     3121 aaaaaaagac aggaacgtct tgcggaagcg gaaaaatttt accgctccaa tatcgaaatg
     3181 ctgcaaaaaa acctgatccg ggaacaggcg gactacgcgc gcgagcaaga cccggacaga
     3241 aaggccgctt tctttttccg catgatgggc attcaggccg acattcaggc cgagcaggac
     3301 aagatcaata ctttgcatac aggcaagatc gttcataccc ggacgcagta tgacgattac
     3361 gtccagaata agtttatcga aaggatcaaa gaagagcagg agaaatacgt caaactggag
     3421 cgggctgccg ccatcatccg gaaggaaacg aaaaagctgc cgccggggga aagagaaaaa
     3481 gccgcggagt ttgtcgataa acacatcaac ggcaaaaccc tggccaatct ggatgaggaa
     3541 accgtcaaaa aggcgggaga ggcaattttc aacaaaacgc agggatacaa cgaagccgag
     3601 gctgccaaat atgaagaaga agcctacatg tgggacgatt atctgacccg ggcagaacgg
     3661 gtgaaaatgg ccgccgacat cggcatggtg ggctgctcct tcctgggggg gccgatggct
     3721 ctgtcttcgg cttatgacat ctcgacgggc tattttcagg ggggcatcaa agaagcggcc
     3781 actcagttag tccttcaaac cggagctttc gccctgggaa aatatgtcgg ggccggctct
     3841 aaggcggcaa caggcgccga tgacgcagcg gcggcagcca aaacggcagc ccgtcagtcc
     3901 ggaaaagttt ccaaaggact ctccagcgcg gaagagctga agatattcca ggccaaccgg
     3961 gccaggggtg aaaaaactgt caaggaattt gagaacttga accaatcgct gaaaaatgcc
     4021 tatttaaaca aagcgtctca aaacgagatc gccgccatca agaagcaaat ccgggaaaag
     4081 acagtcgaaa ttcaggccaa tccccatgca aagaattttc tgaaatatca gggcgataaa
     4141 ctgacacaga cccgctttaa ccggagcatc aaaagcgttc atcaggatgt cgagaagaag
     4201 ttctacgaga agatgagaaa agagccgtat cagtggagtg agtttaaaat caaggaaatg
     4261 agaaacgcct ccagcgcagg ctccgtcggg atggactatg acattggatt gatcgaaaag
     4321 ccgaacttta tcaaactttc ggacggtacg atgaagcgcc aatactggct gatgagggat
     4381 ggcaagcccg ccacgcgcag ccagtggcaa caagaagcgc agaaatgctg gaatgaggcc
     4441 tatgaagagg ttgcgctgaa aaatgcagag cggtcctggg aaaatctgac gacccggatt
     4501 catcccgaga gttatcagga tctggccgtg ctggacggaa acctgaatca ggtttccaag
     4561 gcctggattc agcagacggc cgatgtgacg cgctacaagg cgatgcacat gctcaaggac
     4621 ggctccatgt ccaagtttga aaaacttcag gaaatcagcc gcgggacggc caaggatctc
     4681 aacaccaaac tgatgccgat ctttgaaagc atgcagaaga ccaagcaggt ttcagcggcg
     4741 gccgaacact ggaaaaaggt gtcttccgtg ctggatgatt tcgggaagaa caagatcgat
     4801 cccatcacgg cggagcgtcg catcgccgaa ttaacgggcg ggaaatccat tcccgaagtg
     4861 gtggacgata tgtcctatat gatggagagc tttgtcaaac tggggaagta atcccattta
     4921 acttaatgat atcgacaggc aaaccgcttt cagccgcctt gctgcggaaa tttttcaagg
     4981 gactgaaatt agtagttgac aagtatataa taaatatata gtctgatctc caagcaaaga
     5041 ggaaaagacg gatgtcgatc aaatacgcca ttcttgggct cc
//
LOCUS       DAOSIM010000151         5005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_14973_length_5005_cov_9.522020, whole genome shotgun sequence.
ACCESSION   DAOSIM010000151 DAOSIM010000000
VERSION     DAOSIM010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14973_length_5005_cov_9.522020"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLR47_07215"
     CDS             complement(<1..575)
                     /locus_tag="PLR47_07215"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOG12773.1"
                     /translation="MPENPLRLLMNPRSIAVVGASNDFRKMGTIQALSILQDGYSGDV
                     FFIHRKEKTVLGRPAFPAAADLPKAPDLAVLIVPSSSIAGLLADFGAIGTKRAIIITA
                     GFKETGSAGRSMEEKIKSVASRHGIRFVGPNCLGIINSENSLNTTVAPFTTGPGRLGF
                     ASQSGTYVTQALPYLRKNGIRYSKAISLGNEA"
     gene            756..1388
                     /locus_tag="PLR47_07220"
     CDS             756..1388
                     /locus_tag="PLR47_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOG12774.1"
                     /translation="MGLIRLLISDPAAFVMLAVLLLYSVIFHELAHGWVALRMGDETA
                     KWLGRLTLNPIKHLDPIGSLMLLVIGFGWAKPVPVNLENIPAPQRRKGLILVSAAGVT
                     ANMLIAFFALLLWRLFSPEPGGMIYSVLYTLAYINILLAAFNLIPIPPLDGSKILMGF
                     TPESFNRVLYQIEPFGFFIVLGLLLLGALNPVIAALQYGIITLISMFLPG"
     gene            1410..1988
                     /locus_tag="PLR47_07225"
     CDS             1410..1988
                     /locus_tag="PLR47_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG12775.1"
                     /translation="MMTFSELLQKRRAIRDFEDKAVPLPLVEEILRDATLAPSASNNQ
                     PCRFSVVQCRKAMKNLSDESKACLLKDHGDKKIALNPQYVNLLQDENFNVFYNAPCLI
                     FVAGPKSAGSLDVDAALAAAYIMFAAAARGLGTCWVALGANIRDPQLRGELGFSENLR
                     IVAPIILGYPKAIPAAPERHAPEILRVIAEAR"
     gene            complement(1999..3693)
                     /locus_tag="PLR47_07230"
     CDS             complement(1999..3693)
                     /locus_tag="PLR47_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12776.1"
                     /translation="MQTAGISFLLGPYQNYVANALDKMSRGNIAARIWANDFHVWKPS
                     PAEIANRMGWLHAPVETSAHVGEIRASVEPFTSGGISDVVLLGMGGSSLSAEVFSSVF
                     GSAPGFPRLQILDTTDPVVIRRAARGLNLDKTLFIVSSKSGRTLEIRSLFHYFYNLAD
                     RTPGVSANEHFMFITDEGSPMLTQAWQLQLPYVFSNNPNIGGRFAALSLVGIIPAALI
                     GVSIEKLLQSAMAAANREKADFFSGRGGSTGFLLGAALGTLAQYGRDKLTLLLPPPWK
                     PFGDWLEQLLAESLGKEGKGILPVLNEPPLDVDRYGNDRVFVAFHHDDDEWASRISEL
                     AGAGHPVLKIRINGFYDLGGQMFLWEMATAVAAHIMGINPFDQPDVEATKTHTNRMIE
                     EFRNNKEWPAPRPALSLTACEVYGDVSARTLSSALTRFLAQAGEGAYGGLQVYLSPSP
                     AIDDALQKLRLAISSRYKIAVTTGYGPRYLHSVGQLHKGDAGRGLFIQMTGPNKSDIG
                     IPDELGKPGSSLTFGELKAAQAEADRQALLDKGRKILRIHWKKDAAGGIRTLAGSL"
     gene            complement(3706..>5005)
                     /locus_tag="PLR47_07235"
     CDS             complement(3706..>5005)
                     /locus_tag="PLR47_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOG12777.1"
                     /translation="PNKQGLCDSHGSPLGEKELSGAKEACAWPTEQAFYVSGEVAKFF
                     RRAVSHGRKYEHAWQDALADYRRANPALAAELERTLSGKLPEGWDAKLAEFPAGSKDM
                     ATRKASEAVMQMIASRVPELAGGSADLNPSTCTWLKGMGDFQSPKLGKEGLHGMVGGA
                     WDYSGRNIHYGVREHAMGAISVGLALHGGVIPYTATFLTFADYMRPPMRLAALMGLRV
                     IFVFTHDSVGVGEDGPTHQPVEQIMNLRQIPNMTVIRPADANETLEAWRLALANTTGP
                     TSLIFSRQNLPLLDRSVCAPASCTGRGGYILWESSVLEPNIILMATGSEVVLALEAGR
                     RLAQEGTRVRVVSLPSWEIFDRQPKEYRDSVLPPRISARLAVEAGIKLGWEHYVGLSG
                     KIIGMETFGASAPGPVLYEKFGFTSDGIFAAAKELLTIRG"
BASE COUNT          969 a         1549 c         1479 g         1008 t
ORIGIN      
        1 gcctcgttgc ccaggctgat cgccttgctg tagcggatgc cgtttttgcg cagatacggc
       61 agcgcctgcg tcacataggt gccgctctgc gacgcgaatc ccaggcgtcc cgggccggtc
      121 gtaaacgggg ccaccgtggt gttgagcgaa ttttcggaat tgatgatccc caggcagttg
      181 gggcccacaa accggatgcc gtgccggctg gcgacggact tgatcttttc ttccatgctc
      241 cggccggccg atccggtttc cttgaagcct gcggtaatga tgatggcgcg cttcgttccg
      301 atggcgccga aatccgcaag cagccccgcg atggaggagc tcggcacgat caggaccgcc
      361 agatccggcg ctttcggcag atcggccgcc gccggaaaag cggggcgccc gagaacggtt
      421 ttttccttgc ggtgaatgaa aaacacgtcc cccgaatagc cgtcctggag gatgctcaaa
      481 gcctgaatgg tgcccatttt cctgaaatca ttactggcgc cgaccaccgc tatggagcgc
      541 ggattcatca gcagacgcaa aggattttcc ggcatggttc ctcttccttt tctgaaggtg
      601 atggaatgtg cggaacattc ctacaaatta aacgccttgt ctgtcaagat gaaaactccg
      661 gttgaatatc gcgtcttcat gatttatgct ttccgtcagg ttgaaattcc cggagacaat
      721 cattcgggaa cccgatcaag aagggagaag gggtgatggg tttaatccgc ctgctgatca
      781 gcgatccggc cgcattcgtg atgctggccg ttctgctgtt gtactcggtg atcttccacg
      841 agctggcgca cggctgggtc gccttgcgaa tgggcgacga aacggccaaa tggctgggaa
      901 ggctgacgct gaatcccatc aagcatctgg atcccatcgg gtcgctcatg ctgctcgtca
      961 tcggattcgg ctgggccaaa ccggtcccgg tcaatctgga aaacattccc gcaccgcaaa
     1021 ggcgcaaagg gctcattctg gtttcagccg ccggcgtgac ggccaacatg ctcatcgctt
     1081 ttttcgcgct tttgctgtgg cgcctttttt cccccgaacc gggcggcatg atctattcgg
     1141 tgctctacac gctggcgtat atcaacattc tgcttgccgc gttcaatctg attccgattc
     1201 cgccactgga cggctccaaa attctgatgg gattcacccc ggaatcgttt aaccgtgtgc
     1261 tgtaccagat cgagcccttc ggcttcttca ttgtgctggg cctgctgctt ctgggagcgc
     1321 tgaatccggt gattgccgcg cttcaatacg gcatcatcac gctgatatca atgtttctgc
     1381 cgggataaac gaagaaagga gaaaaaatca tgatgacttt ttccgagctt ctgcaaaaga
     1441 gaagggcaat tcgggacttt gaggacaagg cggttcccct gcctctcgtc gaggaaattc
     1501 ttcgggacgc gacgctggcg ccgtccgcca gcaacaacca gccttgccgc ttttccgtcg
     1561 tccagtgccg caaagccatg aaaaatctgt ccgacgaaag caaggcctgc ctcctgaagg
     1621 atcacggcga caagaagatc gccctgaatc cgcagtacgt gaacctgctc caggatgaaa
     1681 atttcaatgt gttctacaac gcgccctgcc tgatttttgt cgcagggccg aaatcggcgg
     1741 gctctctgga cgtggacgcg gcgcttgctg ccgcctacat catgttcgcg gcagccgccc
     1801 gggggcttgg cacctgctgg gtcgccctgg gggcgaacat ccgcgacccg cagcttaggg
     1861 gcgaactggg gttttccgaa aatctgcgga ttgtcgcgcc gattattctg ggttacccaa
     1921 aagcgattcc cgccgcgccc gaacgccacg cgccggaaat tctccgcgtg atcgccgaag
     1981 cccggtgacc gacgaaggct aaagacttcc ggccagagtc cggattccgc ccgccgcgtc
     2041 ttttttccag tggatgcgaa ggattttccg ccctttgtcc agaagcgcct ggcggtccgc
     2101 ttcggcctgc gcggccttga gctccccgaa ggtcagagac gagccgggtt tgcccagctc
     2161 gtcgggaatg ccgatgtccg atttgttcgg gccggtcatc tggataaaca ggccgcggcc
     2221 cgcgtcgcct ttgtgaagct ggcccaccga atgcagatag cgcgggccgt agccggtcgt
     2281 cacggctatc ttgtaccggc tggaaatcgc cagacgcagt ttctgcagcg cgtcgtcgat
     2341 cgcgggagag gggctgagat agacctgcaa tcccccgtac gctccctcgc cggcctgcgc
     2401 gagaaacctg gtcagggcgg aagaaagagt ccgggcggaa acatcaccgt aaacctcgca
     2461 ggcggtgagc gacagagcgg gcctgggcgc cggccattct ttgttattcc ggaattcctc
     2521 gatcatccgg ttcgtatgcg ttttggtcgc ctcgacatcc ggctggtcga agggattgat
     2581 gcccatgatg tgcgcggcga cggccgtcgc catttcccac aaaaacatct ggccgcccag
     2641 atcgtaaaag ccgttgatcc ggattttcaa gacgggatgc ccggcgcccg ccagctccga
     2701 aatcctcgac gcccactcgt cgtcgtcatg atggaacgcg acaaaaacgc ggtcgttccc
     2761 gtaccggtcc acgtccagcg gcggttcgtt cagaaccggc aaaatgccct tgccttcctt
     2821 gccgaggctt tccgcgagga gctgttccag ccagtcgccg aaaggcttcc agggcggcgg
     2881 aagcagcagc gtaagcttgt cccggccgta ctgcgccagc gtgcccagag cggcgcccag
     2941 cagaaatccc gtggagccgc cccggccgga aaagaaatcc gccttctccc ggttggccgc
     3001 ggccatcgcg ctctggagca gcttttcaat ggaaacgccg atcagcgcgg cgggaatgat
     3061 gcccacgagc gacagagccg caaagcgtcc gccgatgttg ggattattgg aaaaaacgta
     3121 cggaagctgg agctgccacg cctgggtcag catcgggctg ccctcgtcgg tgatgaacat
     3181 gaagtgctcg ttggccgaaa cccccggcgt ccggtcggcc agattgtaaa aataatggaa
     3241 aagcgagcgg atctccagcg tcctgccgga cttggaggaa acgatgaaaa gggttttgtc
     3301 gaggttgagc ccccgcgccg cgcgccgaat gaccaccgga tcggtggtgt ccagaatctg
     3361 cagcctcgga aaacccggcg cgctgccgaa gacgctgctg aacacttccg ccgacaggct
     3421 cgatccgccc atgcccagaa ggacaacgtc gctgatgccg ccgctggtga agggctccac
     3481 ggaggcgcgg atctcgccga catgggcgga cgtctccacg ggcgcgtgga gccaccccat
     3541 ccggttggcg atctccgcgg gcgacggctt ccagacatga aagtcgttgg cccagatgcg
     3601 cgcggcgatg ttgccgcggc tcattttatc cagcgcgttg gcgacgtagt tctggtaggg
     3661 gcccagtaaa aaagaaatcc cggcggtctg cactcatttg ctcctttatc cgcgaatggt
     3721 cagcaattcc ttggccgccg caaaaatgcc gtcggacgtg aacccgaatt tctcatagag
     3781 caccggtccg ggcgcggacg cgccgaaggt ctccatgccg atgatctttc ccgaaagccc
     3841 gacataatgc tcccatccca gcttgatccc cgcctccacg gccaggcgcg cactgatcct
     3901 cggcggcagg accgaatccc ggtattcctt gggctggcgg tcgaaaattt cccagctggg
     3961 cagcgacaca acgcggacgc gggttccttc ttgcgccagt ctgcgtcccg cttccagcgc
     4021 caggacaact tccgaaccgg tggccatcag gatgatgttc ggctcgagga cagacgactc
     4081 ccacaggata taaccgcccc ggcccgtgca ggaggcgggc gcacatacgg agcgatccag
     4141 aagcggcagg ttctgacggc tgaaaatcag cgacgtcggc ccggtcgtgt tggcaagcgc
     4201 cagcctccag gcctccagcg tttcgttggc gtccgccggc cttatcaccg tcatgttggg
     4261 aatctggcgc aaattcatga tctgctcgac cggctgatgc gtcgggccgt cttcgcccac
     4321 gccgacgctg tcatgcgtaa agacgaaaat gacgcgcagt cccattaaag cggccagccg
     4381 catgggcgga cgcatatagt cggcgaatgt caaaaaggtg gccgtgtagg gaatcacgcc
     4441 gccgtgcagc gccagcccca ccgagatggc gcccattgca tgctcgcgca caccgtaatg
     4501 gatattgcgc cccgaataat cccaggcgcc gcccaccatg ccatgcaggc cttccttgcc
     4561 gagcttcgga ctttgaaaat cacccatgcc cttgagccat gtgcaggtcg agggattcag
     4621 gtccgctgat cctccggcca gctccggcac tcgggaggca atcatttgca tcacggcttc
     4681 cgatgccttg cgcgtggcca tatccttgga cccggccgga aattcagcca gtttcgcatc
     4741 ccagccctcg ggcagtttgc ccgaaagagt tctttccagt tcagcggcca gggccggatt
     4801 tgcccgccgg tagtcggcca gcgcatcctg ccaggcgtgc tcgtactttc tcccgtggga
     4861 aaccgccctg cggaaaaatt tcgcgacctc gccggaaaca taaaatgcct gctccgtggg
     4921 ccaggcgcag gcttctttcg ctccgctcaa ttccttttcc cccaggggag agccgtgcga
     4981 atcgcacaga ccctgtttgt ttggg
//
LOCUS       DAOSIM010000152         4980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_15068_length_4980_cov_7.422335, whole genome shotgun sequence.
ACCESSION   DAOSIM010000152 DAOSIM010000000
VERSION     DAOSIM010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15068_length_4980_cov_7.422335"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="PLR47_07240"
     CDS             complement(<1..924)
                     /locus_tag="PLR47_07240"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOG12778.1"
                     /translation="MKKFSLITDFIPSGDQPQAIDELTRGLAAGKEHQVLLGVTGSGK
                     TFTVANVIARVGRPALIIAHNKTLAAQLYEEFKGLFPDNAVEYFVSYYDYYQPEAYLP
                     STDTYIEKDSAINEEIDKLRHEATHALLTRRDVIIVASVSCIYGLGSPEAYLGMIVQL
                     EVGREIPREDLLKRLVEIQYERNDIDFHRGTFRVRGDIVEIFPPYEDEQALRVEFFGD
                     TIEAIAQIDPLRGKKIGSLNQIAVYPGSHYVTTQQNLKRATADIRAELAVTLARYKEQ
                     NLLLEAQRLEQRTNFDLEMMEEMGYCQGIENY"
     gene            complement(924..2993)
                     /locus_tag="PLR47_07245"
     CDS             complement(924..2993)
                     /locus_tag="PLR47_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG12779.1"
                     /translation="MEEKRVRKKIPFRHKSPVYVRKTKRPEAKVFMKPEIAPPLKSVL
                     AKIGKPHPTPFVPDEFQTRALAAIRQNDCLVMAPTGSGKTWIAREGILSVMEKGGRAW
                     YASPLKALSNSKWVEFGLHFDPGNVGIITGDTKENTEAPIIVGTTEILRNQLYDSMHR
                     GEDLRCDLVILDEAHYLGDADRGVVWEEILIYLPVRVNVLLLSATIGNGEEIAAWIES
                     IRKKPCTVIREEKRPVPLYPIFLHPSGCLHPFLEGKKAAPAVYDFFHKSGRGSRKPPD
                     YAGILRVLERFNLLPAIFFLKSRAECDAALACRSGLPPLKSHGSFDGDLSEILERFPS
                     LGGHRQMRALRQAGLAAHHGGQLPVWKLLVEEMMNRGHLRAIFATTTVAAGVNFPART
                     IVLFNSDQFNGRDFAPLSATEFRQMTGRAGRRGQDNIGFMLAVPGRFMELDHVKKMLR
                     AEPEDIFSQLKNDFAMVLNLLLSQTPANVKEIFERSFAAWQQNKSGKRGRSAAERLWE
                     DFCLHLNFLKKERFVDPDGKLTDDGLWAARLRLDHPLLVAECLRKNALPDDEKLLAAA
                     VAVFAYDREDELQLAGGQWAKPLTAALRKVLLAVRPLARRLEVAGFDLPKLHSSAGAA
                     MSCWAQGRDWDDVLHLTGIADGDMASLVLRTADNLRQIASLRDTHPEIAACAMKAREA
                     ILREPVL"
     gene            3047..4291
                     /locus_tag="PLR47_07250"
     CDS             3047..4291
                     /locus_tag="PLR47_07250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG12780.1"
                     /translation="MAGNQKIFWGWYVVAGSFLVMAVNYGARYCFGVFLKPLSLEHDW
                     SRSVISLAACINMAVYSTGAVFIGRMLDRIAPRWIITCGALLAAFGFFLTSQIHTPLA
                     LYLTYGLLVGLGSSGLGVVACSSSVGKWFIKKRGIALGIATMGIGFGTVALTPLIGYL
                     NESLGWRAGLIALGIITGLVGVVISQLLMSKTHPEAHGLLPDGDKTDAAHRPTAPCVV
                     RISTRVIFQDGRFWTIAVGHGLIVMVNMSVFVHQVAYAMDQGMGSLAAAASLAVISIT
                     GFAGQFLFGWLSDRVEDPKYVYVAGIFVLLIGTIFLWRADSVSGLYLSALVYGFGYGS
                     LAPILPVMAADRFGRHVLGSIYGLLTFFIGIGGSIGPLLGGIIYDTFGTYQHLWEINI
                     AILIIMCVVMLTLKKGKPLYGQ"
     gene            4523..4798
                     /locus_tag="PLR47_07255"
     CDS             4523..4798
                     /locus_tag="PLR47_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12781.1"
                     /translation="MKKYMQTALLVGFIVLTTATFGFAQFSAGPESAFPFFHLGCLMI
                     GGLIIVSLKRKYDKLYLSEAIGSFALYAILVALFTAPVVDAIKTMIA"
BASE COUNT          989 a         1450 c         1371 g         1170 t
ORIGIN      
        1 ataattttca atgccctggc agtagcccat ctcttccatc atctccagat cgaagttggt
       61 tcgctgctcc aggcgctgcg cttccagcag gaggttctgt tccttatacc gggccagcgt
      121 cacggccagt tcggcgcgga tatccgccgt cgcccgcttg agattttgct gcgtcgtaac
      181 ataatggctg ccgggataaa cggcgatctg gttgagcgac ccgatttttt tcccgcgcag
      241 cgggtcgatc tgggcaatcg cttcaatcgt gtcgccgaag aattccacgc gcagcgcctg
      301 ctcatcctca tagggaggaa aaatctccac gatatcgccc cggacgcgga aggtgccgcg
      361 gtgaaagtcg atgtcgttgc gctcatactg gatctccacc agacgcttca acagatcctc
      421 gcgggggatt tccctgccca cttcgagctg aacgatcatt cccaggtagg cctcgggcga
      481 acccaggccg taaatgcacg agacgctggc cacgatgatg acgtcgcgcc tcgtcaaaag
      541 cgcgtgcgtg gcctcgtggc gaagcttgtc gatttcctcg ttgatggcgg aatccttttc
      601 gatgtaggtg tcggttgacg gcagataggc ttccggctga tagtaatcgt agtagctgac
      661 aaaatattcg acagcgttgt cggggaacaa acccttgaat tcctcataca gctgcgcggc
      721 cagggttttg ttgtgcgcga tgatgagcgc gggacggccg acgcgcgcga tcacgttggc
      781 aacggtaaag gttttgccgg aaccggtcac gccgagcagc acctggtgct ctttgcccgc
      841 agcaagtccc cgggtcagtt cgtcgatcgc ctgcggctga tcgccgctcg gaataaaatc
      901 cgttatgagt gaaaattttt tcattacagc accggttccc gcagaatcgc ctcccttgct
      961 ttcatcgcgc aggcggcgat ttcgggatgc gtgtcccgaa gcgaggcgat ctggcgcagg
     1021 ttgtccgccg tccgcaggac cagcgacgcc atatctccat cggcgatgcc ggtcaggtga
     1081 agcacgtcgt cccagtcccg tccctgtgcc cagcagctca tggccgcgcc cgccgacgaa
     1141 tgcagcttcg gaagatcaaa gcctgcaact tccagtctgc gggcaagcgg gcgaaccgcc
     1201 agcagcactt tgcgcagggc ggccgtcagg ggcttcgccc actggccgcc tgcaagctgc
     1261 agctcatcct cccggtcata cgcaaacacc gcgaccgccg ccgccagaag cttttcatcg
     1321 tcgggcagtg catttttccg caggcactcc gcaaccagaa gcggatggtc cagcctcagt
     1381 cgcgccgccc ataggccgtc gtccgtcagc tttccgtccg gatcgacaaa gcgctccttt
     1441 ttcaagaaat tgagatgaag gcaaaaatcc tcccacagcc gttcggcggc ggaccggccc
     1501 cttttaccgc tcttgttctg ctgccaggcg gcgaaagatc tttcaaatat ctctttgacg
     1561 ttggccggcg tctgcgacag caaaagattg agaaccatgg caaagtcgtt tttcagttga
     1621 ctgaaaatat cctccggttc ggcccggagc atttttttga cgtggtccag ctccatgaac
     1681 cggccgggaa ccgccagcat gaagccgatg ttgtcctggc cgcgccggcc ggcgcgcccg
     1741 gtcatctggc ggaattcggt tgccgacagg ggcgcaaagt cacggccgtt gaactggtcg
     1801 gaattgaaca gcacgatcgt tctggcggga aaattcacgc cggcggcgac ggtggtcgtc
     1861 gcgaaaatgg cccgcaggtg cccccggttc atcatttcct ccaccagcag cttccagacc
     1921 ggcaactgcc cgccgtgatg cgcggccagc cccgcctggc gaagcgcccg catctggcgg
     1981 tggccgccca gcgagggaaa ccgctccagg atttccgaca gatcaccgtc aaatgaacca
     2041 tggcttttca gaggcggaag gcccgaccgg caggcaagag ccgcatcgca ttccgcgcgc
     2101 gatttcagaa agaagatggc gggcaaaagg ttaaaccgct ccaaaacccg gaggatcccc
     2161 gcgtaatccg gcggcttgcg gctgcccctt ccggatttat gaaaaaaatc gtagacggcg
     2221 ggcgcggcct ttttcccttc cagaaaggga tgaagacagc ccgagggatg caggaaaatc
     2281 gggtacagag gcacggggcg cttttcctcg cggatcaccg tgcagggctt tttccggatg
     2341 gactcgatcc aggcggcgat ttcctcgccg tttccgatcg tggcggaaag cagaagcacg
     2401 ttcacgcgca ccggcagata aatcagaatt tcttcccaga ccacgccccg gtcggcgtct
     2461 cccagatagt gcgcctcatc cagaatcacg aggtcgcagc gcaaatcctc cccccggtgc
     2521 atggagtcgt agagctggtt gcgcagaatt tcggtcgtgc ccacgataat cggcgcttcg
     2581 gtgttttcct tggtgtctcc cgtgatgatg ccgacgtttc ccggatcgaa atgaaggccg
     2641 aactccaccc acttggaatt ggaaagcgcc ttgagcggcg aggcgtacca ggcgcgcccg
     2701 cccttttcca tgaccgacaa gatcccttcc cgcgcgatcc aggtttttcc ggagccggtc
     2761 ggcgccatca ccagacagtc gttttgccga atggccgcca gcgcccgcgt ctgaaattca
     2821 tccggaacga aaggcgttgg atgcggtttg ccgatttttg ccagaacgct tttgagcggc
     2881 ggggcaatct ccggtttcat gaaaactttc gcctccggac gttttgtttt tcgcacgtaa
     2941 accggagact tatgccggaa ggggattttt ttgcggactc tcttttcttc catgattatt
     3001 tgcttgtagc acacccgtta ataatggtaa agcccggccg acggttatgg caggcaatca
     3061 aaaaatattc tgggggtggt atgtcgtcgc cggctctttc ctggtgatgg cggtcaatta
     3121 cggcgcgcga tactgcttcg gcgtctttct caaacctttg tccctggaac atgattggtc
     3181 ccgctcggtc atttcccttg ccgcctgcat caatatggcg gtttactcga ccggcgccgt
     3241 cttcatcggc cgaatgctgg accgcatcgc accccgctgg atcatcacct gcggcgcgct
     3301 tctcgccgcg ttcggtttct tcctgaccag tcaaatccat acgccgttgg ctctgtatct
     3361 cacgtacggc ctgctcgtgg ggctgggctc ttccggactg ggcgtggtgg cctgcagttc
     3421 ttcggtcggc aaatggttca tcaagaaacg cggcatcgcg ctgggcattg ccaccatggg
     3481 cattggtttc ggcacggtgg cgctcacgcc gctcatcggc tatttgaacg aatccctggg
     3541 gtggcgggcg ggccttatcg cgctgggaat catcaccggc cttgtcggcg tcgtgatttc
     3601 acagcttctg atgagcaaaa cccacccgga ggcgcacggt ctgctgcccg acggagacaa
     3661 gacggacgcg gcacaccggc cgacagcgcc ttgtgtcgtc cggatttcca cgcgggttat
     3721 ttttcaggac ggccgcttct ggacgattgc cgtcggccat gggctgatcg tcatggtcaa
     3781 catgtctgtt tttgtgcatc aggtcgctta tgccatggat cagggcatgg gcagtctggc
     3841 ggccgcggca tcgctggcgg tcatcagcat aaccggcttt gccggccagt tcctttttgg
     3901 atggctttcg gacagggtcg aagatcccaa atacgtttac gtggccggta tttttgtcct
     3961 gctcatcggc accattttcc tgtggcgggc ggacagcgtg tccggcctct atctgtccgc
     4021 cctggtgtac ggcttcggat acggatcgct tgctcccatt ctgccggtca tggccgccga
     4081 ccgtttcggc aggcatgttc tgggctccat ttacggcctg ctgaccttct ttatcggaat
     4141 cggcggcagc atcggtccgc ttctgggagg aattatctac gatacattcg gcacttatca
     4201 gcatctttgg gaaatcaaca tcgccatcct tatcatcatg tgcgtggtca tgctgacgct
     4261 gaaaaaggga aaacctcttt acggccaatg aagcccctgc cattcaatat tttattcgtt
     4321 ttttgaccgt cgttgaacgt ttcattaaaa taaaacgaag tgtttcatcc tgcccgacaa
     4381 tcgtgattgt tttgcatatt cttaatattc aaataaattc atatagatat aaaatattat
     4441 cctgatattg ttcctttggc atgcttattt catagtaaaa gacagaaaaa cgaagcaacg
     4501 gtaaaaatgg aggaacggaa tcatgaaaaa atacatgcaa acagctcttt tggtcggttt
     4561 cattgtcctg accacggcaa cattcggttt tgcccagttc agcgccggcc cggagtccgc
     4621 attccccttc tttcatctgg gctgcctgat gatcggcggt ctgattattg tttccctgaa
     4681 gcgcaaatat gacaaactct atttgagtga agccatcggg tcctttgccc tttacgccat
     4741 tctggtggct cttttcacgg cgccggtcgt tgacgccatt aaaaccatga tcgcataaac
     4801 ccttccctcc acatatagta gtaaaagccc ccgcgcacac actggcgggg gtttttacgt
     4861 tggacaagaa aaaacgcttt acagcctccg ggatttcaat tattaaactc ggcgtttagg
     4921 ttttccattc agcaaattct ttctaaagga gataaagata tggctaaacg tgtttacact
//
LOCUS       DAOSIM010000153         4906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_15361_length_4906_cov_8.926819, whole genome shotgun sequence.
ACCESSION   DAOSIM010000153 DAOSIM010000000
VERSION     DAOSIM010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15361_length_4906_cov_8.926819"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PLR47_07260"
     CDS             complement(<1..714)
                     /locus_tag="PLR47_07260"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOG12782.1"
                     /translation="MTLSRVLRISALLLVLFAAVCLASLCIGVVSIPAKDLLSVLAGA
                     FGGSPSSPSEEQIILLNVRLPRILFAGIVGASLSSGGVVFQALLRNPLADPYVLGISG
                     GSALGAIIGIVLGAGSFCLGVPFLAFCGALATVFLVFFVAGGSRGLLLDNSMLLAGVV
                     VNAFFSAAILFALSVVNSMELHSITFWLMGDLSGADGKEMILAALCLLAGFGILYAQA
                     RKLNLMVQGEETALQMGVNV"
     gene            complement(865..1662)
                     /locus_tag="PLR47_07265"
     CDS             complement(865..1662)
                     /locus_tag="PLR47_07265"
                     /inference="COORDINATES: protein motif:HMM:NF018525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG12783.1"
                     /translation="MKKRWLLLIVIMILGGCDMQNRFLYFPNDDRPTAAMLASEKMAF
                     WRPAGQDYRGLISTGNAHAPSGTIVLFHGNGGTAFDRGFYLEPLGNLGFRVILAEYPL
                     YGGRPGKVGEKSFVADGLETFRLAHEDYGEPVYLLGESLGCGVAAAVARQTSVPIAGI
                     ILITPWDTLASVAKSLFPFLPVKLVLTDRYDSIENLRNFPRKIAVVGAQYDEILPIRH
                     AHQLYESLPEGRKRMWVIQNAGHNDWPFHADASLWKEITDFVKNENR"
     gene            1783..2697
                     /locus_tag="PLR47_07270"
     CDS             1783..2697
                     /locus_tag="PLR47_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HOG12784.1"
                     /translation="MNIRHFLWNKIAVSVKLRPMNSNWLLADTPETVLLAAEHLRKAS
                     ILGIDTEYDSFRYFREKLCLIQIYSPEATYVLDATAGFDFSFLARIFKSRSAVKVLHA
                     ADNDIRLLKRDYGFEFQNIFDTHRAAMILGFQQLSLDKMIREFLGVELKKSKKMQRSR
                     WDHRPLTGEQIEYAVQDVTLLPALYEKQKAALREKGLEASAKEAFAKIASAHWQERAF
                     DRRGYSKIKGYYALAGEQKELLKKLYVWRFERAREANCAVFMFLSDEKLAELAQNKGG
                     IQDILSPEKYQRYGNDLKRLLAPGAGPA"
     gene            complement(2793..2878)
                     /locus_tag="PLR47_07275"
     tRNA            complement(2793..2878)
                     /locus_tag="PLR47_07275"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2842..2844),aa:Leu,seq:gag)
     gene            complement(2956..3339)
                     /gene="secG"
                     /locus_tag="PLR47_07280"
     CDS             complement(2956..3339)
                     /gene="secG"
                     /locus_tag="PLR47_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOG12785.1"
                     /translation="MTTLLTIIHVLACITLILIVLLQAGKGANMGAAFGGSSQTVFGS
                     SGAGTFLGKMTAAVAIIFMLTSITLTYTASRKTSGLMDGSVAPATRQELPASQKPAQL
                     PALPSASPAAAPGTANMPPASPAAK"
     gene            complement(3569..4225)
                     /gene="nth"
                     /locus_tag="PLR47_07285"
     CDS             complement(3569..4225)
                     /gene="nth"
                     /locus_tag="PLR47_07285"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOG12786.1"
                     /translation="MKDENIHPIVRILKKELDLGEMPIVSHLADSERDPFVILMSTLL
                     SLRTKDEVTAVATDRLLALAVTPDAMLKVPRSKIAKAIYPVGFYRNKAKTIHHVCREL
                     IERFGSKVPDTLDDLLSLKGVGRKTANLVITLAYGKEGICVDTHVHRISNRLGYVKTK
                     TPDETEMALRGKLPRKHWIIYNTLQVFFGRKICKPVSPVCSACPIRKYCDRVDVNRSR
                     "
     gene            4552..4836
                     /locus_tag="PLR47_07290"
     CDS             4552..4836
                     /locus_tag="PLR47_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG12787.1"
                     /translation="MQKTKKYPISQTDEISRRKKKMGQVIVIEDRCKGCSFCIANCPR
                     QVLRLSTVFNKKGYHPPEVVDTTRCVNCHFCEILCPEFAIYSIEFDDFEE"
BASE COUNT         1213 a         1346 c         1307 g         1040 t
ORIGIN      
        1 gacattgacg cccatctgca gagccgtttc ctcgccctgc accatcaggt tgagtttgcg
       61 cgcctgcgcg taaagaatcc caaaacccgc cagcaggcac aaggccgcca ggatcatttc
      121 ttttccgtca gcaccggaca gatcgcccat cagccagaag gtgatgctgt gcagctccat
      181 gctgttgacg acggacaggg caaagagaat cgcggcggaa aaaaaggcgt tgaccaccac
      241 gccggccaaa agcatcgagt tgtcgaggag gagcccccgt gatccgccgg ccacgaaaaa
      301 caccagaaaa acggttgcca gcgcgccgca aaaagcaaga aacggcaccc ccagacaaaa
      361 ggagcccgcg cccagaacaa ttccgataat cgcccccagc gccgatccgc cggaaatacc
      421 cagcacataa ggatcggcga gcggattgcg cagcagcgcc tgaaaaacca cgccgccgga
      481 agataaagac gcgccgacga tgccggcaaa taatattcgc ggcagccgca cgttcagtaa
      541 aatgatttgc tcttcagacg gggaggaagg agatccgcca aacgcgccgg cgaggaccga
      601 aagcaggtcc ttcgcgggaa tcgaaacgac gccgatgcaa agcgacgcca gacagacagc
      661 cgcgaacaaa accagcaaaa gcgcgctgat tctcagaact ctcgataacg tcacccggcg
      721 gtaaatggac ttgttcctcc aataaaattt cccgccccct cacgggccgg acaaaaaggg
      781 aggagtttcc gtagtacatg tccggcaaaa ttgcaacgcg gaaacgctca gcgcatgccg
      841 gtgagggcca ggtagtggat gattctaccg gttttcattt ttaacaaagt ccgttatttc
      901 tttccacagc gaggcgtccg catgaaacgg ccagtcattg tggcccgcgt tttggatcac
      961 ccacatgcgt tttcttcctt cgggcaggct ttcgtagagt tgatgcgcgt gccggatggg
     1021 cagaatttca tcgtactgtg cgccgaccac ggcgatcttg cgggggaaat tgcgcagatt
     1081 ttcaatgctg tcgtaccggt cggtgaggac cagcttcacc ggcagaaatg gaaaaagcga
     1141 cttggccacg gacgcgagcg tgtcccacgg cgtaatcaga atgatcccgg caatcgggac
     1201 ggaagtctgt ctggcgacgg cggcggccac accgcagccc agtgattcgc ccagaagata
     1261 aaccggttca ccatagtctt catgagccag acgaaacgtc tccaaaccat cggcgacaaa
     1321 agacttttcg cccacttttc ccggacggcc tccatacagc ggatactcgg ccagaatgac
     1381 cctgaaaccg aggttcccga gcggctccag ataaaaaccg cggtcaaaag ccgtgccgcc
     1441 gttgccgtga aacaacacga tggttccgga aggcgcgtgc gcgttcccgg tggaaatcag
     1501 tcccctgtaa tcctgacccg ccggccgcca gaaggccatc ttttccgacg ccagcatggc
     1561 cgccgtcggc cggtcgtcgt tgggaaaata gagaaatcgg ttctgcatgt cacagcctcc
     1621 caaaatcatg atgacaatga gaagtagcca tcgttttttc atagaccggc atactccctt
     1681 gacgcgtccg tgaaacgtcg ggaaaaactt tgaggaaaac gtagcacatc tgatgatttt
     1741 gtaaagacct tcctgctttt tgccgcgaga cggaaggcca ttttgaacat ccgtcatttt
     1801 ttatggaata aaatcgccgt ttcggttaag ttgcgcccca tgaactcgaa ctggctactg
     1861 gcggacacgc ccgaaaccgt cttgcttgcg gcggagcacc tgcgaaaagc gtcgatactg
     1921 ggaatcgata cggaatacga ttcctttcgt tatttcaggg aaaaactctg tcttatccag
     1981 atttattccc ccgaagccac ttacgttctg gacgcaacgg ccggctttga cttctccttt
     2041 ctggccagaa ttttcaaaag cagatccgcg gtcaaggtgc tccacgcggc cgacaacgat
     2101 attcgtctgc tcaaaagaga ctacgggttt gaatttcaaa acatttttga cacgcaccgg
     2161 gccgccatga tcctggggtt tcagcaactg tccctggata aaatgattcg ggaatttctg
     2221 ggcgtggaat tgaaaaaaag caaaaaaatg cagcgttccc gatgggacca tcgtccgctg
     2281 accggcgagc agatcgaata tgccgtccag gacgtgacgc tgctgcccgc cctttatgaa
     2341 aagcagaaag cggctctccg tgaaaaaggg ctcgaggcgt cggccaaaga agctttcgcg
     2401 aaaatcgctt ccgcccattg gcaggaaaga gcgtttgacc ggcgcggcta ctcgaaaatc
     2461 aaaggatatt atgcgcttgc cggcgaacag aaagaactgc tgaaaaaact ctacgtctgg
     2521 cgctttgaac gggccagaga ggccaactgc gccgtcttca tgtttttgtc ggatgaaaag
     2581 ctggccgaac tggcgcaaaa caagggcggc attcaggata ttttatcgcc ggaaaaatac
     2641 cagcgctacg gaaatgacct gaagcggctg cttgcgcccg gcgcagggcc ggcctgaccc
     2701 tgcaaccgcc tgaaacaaaa agcacctttt ctgctgtttt tacaaaaaaa gagggatcgg
     2761 ccggtgacgg ggccgatccc tctttatatt tatggtgccg aagactggat ttgaaccagc
     2821 acgggtgtaa cccactaccc cctcaagata gcgtgtctac caattccacc acttcggcat
     2881 gaaggaaaac tcggacgctg cgggccggcc gcacgaagaa aatgcctgaa acaccggcca
     2941 atctgaaaag aaccatcact tcgccgccgg agacgccggg ggcatattcg ccgtgccggg
     3001 cgcggccgcg ggagacgcgg agggcaacgc cggcaattgc gccggttttt gcgatgcggg
     3061 aagctcctgc cttgtcgcgg gagccacgga tccgtccatc agaccagagg ttttccgcga
     3121 cgcggtatag gtcagcgtaa tcgatgtcag catgaaaatg atcgcgaccg ccgccgtcat
     3181 tttccccaaa aaggttccgg ccccgctgga gccgaaaacg gtttgactgg acccgccgaa
     3241 cgcggcgccc atgttggcgc ctttgcccgc ctgcagcaaa acgatcagta tcaacgtaat
     3301 gcaggccaga acatggataa tcgttaataa cgttgtcatt tttatatcct ttttaaatct
     3361 cctcagtgat cgggattcat atcacccgcc ggtgatttat tcaacgcttc ttttgcttac
     3421 ggatcagcaa tgaattcttc aataaaaatc atcatgtccc gcatggagtg cccgcgcctg
     3481 cccgaaagcg gcggcatcat agctaaatga cgggaaatcg tcaacttttt tcttcaatcg
     3541 aattacctgt cacccattac ccaaatcatt acctgcttcg gttcacatca acccggtcgc
     3601 agtactttcg aatcggacag gcgctgcaaa cgggcgagac gggcttgcag attttccggc
     3661 cgaaaaacac ctggagcgtg ttgtagatga tccagtgttt gcggggaagc ttgccgcgca
     3721 gggccatttc cgtttcgtcg ggcgtttttg ttttcacgta gcccaggcgg ttggaaatcc
     3781 ggtggacgtg cgtgtccacg cagatgcctt ccttcccgta cgccagcgtg atcaccagat
     3841 tcgccgtttt ccgcccgacg cccttgaggc tcagaaggtc gtccagtgtg tccggcactt
     3901 tcgaaccgaa ccgttcgatc agctcccggc agacatgatg aatcgttttg gccttgttgc
     3961 ggtaaaatcc caccggataa atggctttgg cgattttgga gcgcggaacc ttcagcatgg
     4021 cgtcgggcgt cacggcaagc gccagaagac ggtcggtggc aacggccgtg acttcgtctt
     4081 tggtgcgcag gctcagaagc gtggacatca ggatgacaaa cgggtcgcgc tcgctatcag
     4141 ccagatggga gacaatgggc atttccccca gatccagctc cttcttcaga attctgacga
     4201 ttggatggat gttttcgtct ttcatgtttt gggtctgctt accgtgcaag tttcctcagg
     4261 ttgttgccca aaggtattat gacgagactt gtcgaaaagc aagtttttat ccgcaaatcc
     4321 ttcaggacag tgatcggagt atggaaaatt attaacaaag gaccgttttg gatggtcgtg
     4381 ttttttcttg acagctcttt tggtttccta taatgccctt tttgcacctt tgcgcgcgca
     4441 agatggagtt tggcacttca gggaccaggg cttccccgat catcaagcaa attcaatcca
     4501 tccggcggaa tgccccgcac agcacgaaca cgcgggtgaa ataaaacttt catgcagaaa
     4561 acaaaaaaat atcctatcag tcagacggat gagatcagcc gccggaaaaa gaaaatgggg
     4621 caggtgatcg tgattgaaga ccgctgcaag ggatgcagtt tttgcattgc caactgcccc
     4681 cggcaggttc tgcgtctttc caccgttttc aacaagaagg ggtaccatcc gccggaggtg
     4741 gtggacacca cccgttgcgt caactgccat ttctgcgaga ttctttgtcc cgaatttgca
     4801 atttacagca tagaatttga cgatttcgaa gagtgagccg tagaaggaga gtggctttga
     4861 ctgctaaaga agtaagaaca ggaacgcatt tcatgaacgg cgacga
//
LOCUS       DAOSIM010000154         4905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_15366_length_4905_cov_7.099175, whole genome shotgun sequence.
ACCESSION   DAOSIM010000154 DAOSIM010000000
VERSION     DAOSIM010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15366_length_4905_cov_7.099175"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PLR47_07295"
     CDS             <1..776
                     /locus_tag="PLR47_07295"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOG12788.1"
                     /translation="GLSIATGATSRRIAYSTGIILILLAFFPKFASIFVIMPTPIMGA
                     SLIFAVSFMILAGLQIIMSRMIDARKTFVIGISIIFGLSVEFAPGLYRDVHPWIKPIF
                     SSSLSLATICAVVLNLIFRIGIAKTTSLVLTPGENPSEDIDHFMEEQGRAWGARSEVI
                     YKAKAAMIEFYEAAAESALTRKPVTMTARFDEFNLDVDMDYEGKPIELAASRPDARDL
                     LTDPDAPLKLAGYMIRSLADHAAMESVAGRNRVHLHFNH"
     gene            complement(872..2074)
                     /locus_tag="PLR47_07300"
     CDS             complement(872..2074)
                     /locus_tag="PLR47_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12789.1"
                     /translation="MTYYVLIAFCVIILLAYIFDITAKYTRIPGVILLIATGMFINYL
                     ASSFKIGIPDMSGMLPIMGTLGLILIVLEGSLDLAIRRDKSSLIIGSVSSAIVLFAVS
                     IGVFTYVAVYFLHYDLQASLLNIIPFAVISSAVAIPSAASLNPEDREFVTYESSISDI
                     IGILAFDFILLSSGSLGAGVLRFIAELILTFIASILLSGGLAFLLHKISHHVKYVIIM
                     TAIILVYALAKLVHLPSLLVVLIFGLVMNNNVLFKNEFSAKWINFDEFNADLGAFKNI
                     VFELTFIIRCFFFILFGFYTRLSDLLNLNNLMIAAAITASIFIFRALFFRIVLRLPLN
                     PLLYFAPRGLITILLFLSIPSILLLPFINVGIVTQVIFLTILMMMFGNMIFSKDKTPE
                     APETPIRR"
     gene            complement(2093..2773)
                     /locus_tag="PLR47_07305"
     CDS             complement(2093..2773)
                     /locus_tag="PLR47_07305"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOG12790.1"
                     /translation="MRYIVIGLGFFGSSLAAKLTSLGHEVIGIDRRSERAEELKDKIT
                     NVMIMDCTKPAALSALPLSDVDAVIVAVGEDIGSSVLILSMLKKQKVKRIIGRAINPM
                     HQNILGELGIEEIIHPEEDTATELTAMLMVQNAVNTMIVNDKIIIAELKVPDAYVGHT
                     LEAANLENRFGIRLVAVKIAPAQKSLSTLLGKEYEVDLSGDPHRPLGAKDRLIVIGRL
                     NDIRRFVD"
     gene            complement(2782..4596)
                     /locus_tag="PLR47_07310"
     CDS             complement(2782..4596)
                     /locus_tag="PLR47_07310"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOG12791.1"
                     /translation="MSYFDNEALFKKFYVASDWANTVGSAIIALAGFVFALSTLLFGS
                     LILFDFGFRKTAADSLAVHQTYLLLAAALFASKLLIELLAFRAAKWFAVVFRVLVLAV
                     VFVILLAHLHAFPEAWSVISGALGGRTALLLGSLALMATQMHHLSGTLNRINISPSFL
                     FAGSFLAMILAGAGLLMLPNATLQPISAYEALFTATSAVCVTGMMIVDPSAVFSPMGK
                     NIILGLIQTGGLGIMTFTGFFSYLFLGSASLKDRFLLKDFFSGDQLGGLYKLLLKILL
                     LTILVEAAGSVLIYRTLEGDWMHKTHNAVFHAVSAFCNAGFSVNPQEFNALHLQSNFS
                     LQFILCGLVVLGGIGFPLLLYFYKVLKFNVKSIFFRVVGRNLPHEVLLLAVGERLALH
                     ATLVLLVLGAFLYYALEAGSPLPEHFNWSQGMTALVSSVNARTSGFSMTDPGGWSYPT
                     VFLTIFLMWVGASPGSTGGGIKTTTLAVAVKTVASFLRGKDRLEIGNREIGSSTIVRA
                     FSVIVLSLMFIFAGFLLLMIFDPGRNPVHLLFECVAAFSTTGLSIAGTMTLSTPSQVV
                     LMILMFVGRIGPVIVLSGFLATQTQNLYRLPVENIKMN"
BASE COUNT         1186 a         1316 c         1444 g          959 t
ORIGIN      
        1 tcgggctttc catcgccacg ggcgcaacca gccgccgcat cgcctacagc accggaatca
       61 tcctgattct tctggccttc ttccccaagt ttgcctcgat cttcgtcatt atgcccacgc
      121 ccattatggg cgcctcgctg attttcgccg tgagcttcat gattctggcc ggtctgcaga
      181 tcatcatgtc ccgcatgatc gacgcgcgga aaacctttgt catcgggatt tccatcatct
      241 tcggactcag cgtcgaattt gcccccggtc tttaccggga tgtccacccg tggatcaaac
      301 cgatattcag ctcttccctg tcgctggcca cgatctgcgc ggtggtgctg aatctgatct
      361 tccgcatcgg catcgcgaaa accacctccc tggtcctgac ccccggcgag aatccgtccg
      421 aagacatcga ccatttcatg gaggagcagg gccgcgcctg gggcgcccgc agtgaagtca
      481 tctacaaggc gaaggcggcg atgattgaat tttacgaagc cgccgccgaa tccgctctga
      541 cgcgaaagcc cgtcacgatg accgcccgct tcgacgagtt caacctggat gtggacatgg
      601 actacgaggg aaagcccatc gaactggccg cctcaaggcc cgacgcgagg gacctcctga
      661 cggatccgga cgccccgctc aaactggccg gctacatgat tcgaagcctt gcggatcatg
      721 ccgcgatgga gtccgttgcg ggccggaacc gcgtgcatct ccacttcaac cactgatttc
      781 accggttccc gcgagccgtc tcttcgcgct ttgctgaaca tcggatcaac gacggacatg
      841 gaaagcggaa gaaagccgcg gattcgtcgg gtcagcgccg gatcggtgtt tccggggcct
      901 ccggtgtttt gtctttgctg aaaatcatat tgccgaacat catcatcaga atcgtgagaa
      961 aaatgacctg cgtgacgatg cccacgttga tgaagggcaa aagcaggatg gagggaatgc
     1021 tgaggaacag cagaatggtg atcagccccc ggggagcgaa atacagcagg ggattgagcg
     1081 gcaggcgcag gacgattctg aagaaaagcg cccggaaaat aaaaatggac gcggtaattg
     1141 cagccgcgat catcaggttg ttgaggttga gcaggtcgga cagccgggtg taaaatccga
     1201 acaggataaa aaagaagcag cggatgataa aggtcagctc gaatacgata ttcttaaagg
     1261 cgccgagatc cgcgttgaat tcatcgaagt tgatccattt tgcggagaat tcgtttttga
     1321 aaagaacgtt gttgttcatg accaggccga agatcaggac gacgagcagc gacggcaggt
     1381 gaaccagctt ggccagcgcg taaaccagaa tgatcgccgt catgatgatg acgtatttga
     1441 cgtgatggct gatcttgtgc agcagaaagg ccaggccgcc gctcaggagg atggatgcga
     1501 tgaaggtcag gatcagctcg gcgatgaacc gaagcacgcc tgctcccagg gaaccgctgc
     1561 tcaggaggat gaagtcaaac gcaaggatgc cgatgatgtc ggagatggag ctttcgtaag
     1621 tgacaaattc ccggtcttcc ggattcagac tcgccgccga gggaatggcg acggcgctgc
     1681 tgatcacggc aaagggaatg atgttgagca gggaggcctg gaggtcgtag tggagaaaat
     1741 acaccgccac gtaagtgaag acgccgatcg agacggcaaa aagcacgatg gccgatgaaa
     1801 cggagccgat gatgagactg ctcttgtcgc ggcggatggc caggtcaagg cttccttcca
     1861 gaacgatcag gatgagtccg agggtgccca tgatgggaag cattccggac atgtccggaa
     1921 tgccgatttt gaaggaagat gccaggtaat tgatgaacat gccggtcgcg atgagcagaa
     1981 tcacgcccgg aatcctggtg tatttggcgg tgatgtcgaa gatgtatgcc aggagaatga
     2041 tgacgcagaa ggcgatgaga acgtaatagg tcatggcgtt ttgtcctccg gactaatcca
     2101 cgaagcgtct gatgtcgttg agtctgccga tgacgatcag ccggtctttc gcgcccagcg
     2161 gacggtgcgg atcacccgac agatccactt cgtactcttt gccgagcagc gtggaaaggc
     2221 tcttttgggc cggtgcgatt ttgacggcca ccaaccggat gccgaagcgg ttttcgaggt
     2281 tggccgcttc cagcgtgtga ccgacgtatg cgtcaggcac cttcagttcg gcgatgatga
     2341 ttttatcatt gacaatcatg gtgttgacgg cgttttgcac catcagcatg gcggtcagct
     2401 ccgtggcggt gtcttcttcg ggatggatga tttcctcgat gcccagctcg ccgaggatgt
     2461 tctggtgcat gggattgatg gcccggccga tgatccgttt cactttctgt tttttcagca
     2521 tggatagaat cagcacggaa gaaccgatgt cctctccgac ggcgacgatg acggcgtcga
     2581 cgtccgaaag cggcagagcc gaaagcgcgg cgggcttggt gcagtccatg atcatgacgt
     2641 tggtgatttt gtccttgagc tcttcggcgc gctcgctccg gcgatcgatg ccgatgactt
     2701 cgtggcccag cgacgtcagt ttggccgcga gcgacgaacc gaaaaatcct aaaccgatga
     2761 cgatgtatct cattttgccc ttcaattcat tttaatgttc tccaccggaa gacggtagag
     2821 gttttgggtt tgggttgcaa gaaagcccga taacacgatg accgggccga tacggccgac
     2881 aaacatcaaa atcatcagaa ccacctggct cggcgtgctg agcgtcatcg taccggcaat
     2941 ggacagtccg gtggtggaaa aagcggccac gcattcaaac agcaggtgca cgggatttcg
     3001 gcccggatca aaaatcatca acagcaggaa cccggcgaaa ataaacatca gcgacagcac
     3061 aatgaccgaa aacgccctca cgatggtgga ggagccgatt tcccggttgc cgatttccag
     3121 ccggtctttc ccccttaaaa aagacgccac cgttttcacc gcgaccgcaa gcgttgtggt
     3181 cttgatgccg ccccccgtcg atcccgggga agcgccgacc cacatcagaa agatcgtcag
     3241 aaacaccgtg ggatacgacc accctcccgg atccgtcatg ctgaagccgg acgtacgcgc
     3301 gttgacgctc gaaacgagcg ccgtcatccc ctgcgaccag ttgaagtgct ccggcagggg
     3361 gctgcccgcc tccaatgcat aatacaaaaa cgctcccagc accaatagca caagggtcgc
     3421 atgcagcgca aggcgctcgc cgacggccag aagcaggact tcgtgcggga ggtttctgcc
     3481 gaccacccgg aaaaatatag attttacgtt gaacttgagg actttgtaga aataaagcaa
     3541 aagaggaaag ccgattcccc ccagaacgac cagcccgcag aggatgaact gcagggaaaa
     3601 attcgattga agatgcagcg cgttgaactc ctgcggattg accgaaaaac cggcgttgca
     3661 gaaggcggaa accgcgtgaa agacggcgtt gtgggttttg tgcatccagt cgccttccag
     3721 cgtgcggtaa atcaggaccg aacccgccgc ctccaccaga atcgtgagca gcaggatttt
     3781 cagcaggagt ttgtacaggc cgcccaactg atcgccggaa aagaaatcct tcagcagaaa
     3841 ccggtctttc agggaggcgg agcccagaaa gaggtaactg aaaaatcccg taaaggtcat
     3901 aatgcccagc ccgcctgtct ggatgagccc cagaatgatg tttttcccca tgggggaaaa
     3961 gactgcggag gggtcaacaa tcatcatgcc ggtcacgcat accgcgctgg tggccgtgaa
     4021 aagcgcctcg taggcggaaa tgggctgaag cgtggcgttg ggcagcatca aaagtccggc
     4081 gccggcgaga atcatggcca gaaaacttcc ggcaaaaaga aaagaggggg aaatattgat
     4141 ccgattcagg gtgcccgaca ggtgatgcat ttgcgtggcc atcagcgcga gcgatcccag
     4201 cagaagagct gtccggccgc ccagcgcgcc ggaaatcacg gaccaggcct ccggaaatgc
     4261 atgaaggtgc gcaagcaaaa tcacaaagac aaccgccaga accaggacgc ggaacaccac
     4321 ggcgaaccat tttgcggcgc gaaaggccag cagttcgatg agcagcttgg aggcaaacaa
     4381 tgccgccgcg agaagcaggt aggtctggtg gacggccagg ctgtccgccg cggtttttcg
     4441 gaagccgaaa tcaaaaagga tcaggctgcc aaagagcagc gtggacaggg caaagacaaa
     4501 gccagcgagc gcaataatcg ccgatcccac cgtgttcgcc cagtcggacg caacatagaa
     4561 ttttttaaac agggcctcgt tgtcgaaata gctcatgggg tgaacggtct ttcttcttgc
     4621 ggttcttcgg gggaaagtgt agcacaggtc ctgaaaatta tcagtgaaaa atcccggggg
     4681 ttgggtaacg taatccgtcg tggcgcgcat ccggagtgat tcaacggaaa tctgaacgaa
     4741 tgaatttttc aggaggctaa tgattcgact gctcggcaac cgcaatgtaa tctttattct
     4801 ggccatcgta ctcggcctcg cgctgccgca acccgcccgg tggacggtgc tggcgatgat
     4861 gcccgcgctg gcgctggtca tgacgctggc caccatccat gttcc
//
LOCUS       DAOSIM010000155         4904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_15371_length_4904_cov_7.696638, whole genome shotgun sequence.
ACCESSION   DAOSIM010000155 DAOSIM010000000
VERSION     DAOSIM010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15371_length_4904_cov_7.696638"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PLR47_07315"
     CDS             complement(<1..343)
                     /locus_tag="PLR47_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HOG12792.1"
                     /translation="MKYYPVFWNITGKKCVVIGGGEVAARKVARLLDCGADVHVVSPL
                     LVPELADLKKQGRIRHIAGEYADEHLSGAALVIGATDDEEINASISRDAKVRCIPVNI
                     VDDPQKCDFILP"
     gene            555..1754
                     /locus_tag="PLR47_07320"
     CDS             555..1754
                     /locus_tag="PLR47_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOG12793.1"
                     /translation="MLTEKNLEHYADVLLWALATAKKSVLKKNDIVLIQYDRPALALA
                     EILFDRLMDMGVHPIQRMAATFRMEKSFYRKANSKQLTFLAPGDKELYRHIHGRLFLH
                     APESLTHLRDVDPAKIGKTMVARKPLKAILDQREDLRKYSWTLCLLPTEELARQAKMP
                     LVQYARQVIRGCYLDKKEPVSEWKAIHREVSQIKKALSSLAVQCFHLESDHMDLRITP
                     GEQRKWAGVSGHNVPSFEVFVSPDWRGTEGVYYANLPSFRSGNYVKDVRISFAKGKAV
                     AVEAAQGEAFVKKQLAMDPGACRLGEFSLTDRRFSRIDRFMAETLFDENFGGKEGNCH
                     VALGASYSDTYSGDPARLTPALKKKLGFNDSALHWDLVNTEPKTVSAHLKNGKKIMIY
                     DRGIFTI"
     gene            1937..2122
                     /locus_tag="PLR47_07325"
     CDS             1937..2122
                     /locus_tag="PLR47_07325"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HOG12794.1"
                     /translation="MFNFRSPSFKKLSLDPESLQDKDMVERMLQEPRLIRRPVVKIGR
                     KVYFGASADVLADILKS"
     gene            2172..2591
                     /locus_tag="PLR47_07330"
     CDS             2172..2591
                     /locus_tag="PLR47_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOG12795.1"
                     /translation="MKYLTAIEARQFAEKWLPAWTGNHPELLAGFYSEDAFYMDPAVP
                     RGVRGKNNLLAYFRKLLAVNPNWVWTQIEGIPLEDGFLNKWLAKIPVGSVTLEVVGVC
                     FVQLDDAGKIRRNEVYFDRSALLSEMAKLKRSAPAGP"
     gene            2641..3738
                     /locus_tag="PLR47_07335"
     CDS             2641..3738
                     /locus_tag="PLR47_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOG12796.1"
                     /translation="MMEWERLIREARECLQVSPEATARYIKAKDILLEEVNARMTAHP
                     GISNLTGGNPMSMMRDNHSNHLDFMSTVFEFNSFELLVKTVPWVYRSYHSHGFSFDYF
                     PLELEAWKKAVSQFLSPEAAGEINAVYDWMLKNHERMIELSAFIPDQRETYPELKDER
                     RRFGGGLLAADFPLCMKIADSVLERENGQEALYLGLIQPVMYEIGRLWEQDKISAAEE
                     HMATSMVGRILAGLYARFPVPPANRGKAVVTSAPNEFHELGARILADMLEADGWDIFF
                     LGANTPAEELIKLVKKADPRFLAISATMPFNMDRVAAMISGIRNDPALNSVKILVGGA
                     AFNTDHTLWRRIGADAWAEDPPSAIRQVQSW"
     gene            3732..4694
                     /locus_tag="PLR47_07340"
     CDS             3732..4694
                     /locus_tag="PLR47_07340"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOG12797.1"
                     /translation="MVRDSGAPTDLESLLQGNPEKVTRYLLSSGRMAMILLDRKGVIL
                     DCNAFFLESVGLSEKPVGKSIQAFLREGLQAGWECIRGDCRPVKLTFTLNGPLEYFLS
                     GHILNVQEHYLLFAHTVRLTDHEMVVKLSRLTDELTDLTRQLNKKNRELEAANETITR
                     LMNTDPLTGLTNRRQLKERMDAELSKARRHGHPLSALMTDIDHFKSINDTYGHAAGDA
                     VLVGVASILRQTCRKEDIVARYGGEEFVVLLPDSPAAAALDCAERIRKAIEGNVFPEI
                     DRKVTVSLGATLFAPTDTEDSFIGRADAALYEAKTSGRNRVVLK"
BASE COUNT         1184 a         1403 c         1318 g          999 t
ORIGIN      
        1 agggcaaaat aaaatcgcat ttttgcgggt catccacgat attgacggga atgcacctga
       61 cctttgcatc ccgggatatg gaggcattga tttcttcgtc atccgtcgcc ccgatcacca
      121 gagcagcccc ggacagatgc tcgtccgcat attcaccggc gatgtgcctg atccggccct
      181 gtttttttaa atccgccaat tcggggacaa ggagggggct caccacatgc acatccgctc
      241 cgcaatccag aagacgcgcc acttttctgg ccgcaacctc tcctccgccg atcacgacgc
      301 attttttccc tgtaatattc caaaacaccg gataatattt caagtttttc ctttcaggcc
      361 caaaccgtgg cggactcgcg ccgcatgcgt caagctagca tgaagggatg caaaattcaa
      421 gcgggggttt gaccgggcgg attttttatc tatccggaat cagttaatta ttgacactgc
      481 cgccgctttt cgcatatggt tttttcgcaa atgccgccga tgcggcaaaa ggatcgcttg
      541 aggaaatcat aaatatgctt acggaaaaaa atcttgaaca ttacgccgat gtcctcctct
      601 gggcgcttgc cacggcaaaa aaatccgtcc tgaagaaaaa tgacatcgtt ttgattcagt
      661 acgaccggcc cgcgcttgcg cttgccgaaa ttcttttcga caggctgatg gacatgggcg
      721 ttcaccccat tcagcgaatg gccgccacct tccgcatgga aaaaagcttt taccgcaaag
      781 ccaattcgaa acaattgacc ttcctggccc ccggcgacaa agagctttat cgtcacatcc
      841 acggccggct ctttctccat gcgccggaat ctctgaccca tcttcgggac gtcgatccgg
      901 ccaaaatcgg gaaaacgatg gtcgcccgaa aacctctcaa agccattctc gaccaaagag
      961 aagatctgcg caagtatagc tggacgctgt gcctgctgcc gacggaggaa ctggcgcgcc
     1021 aggccaaaat gccgctcgta caatacgccc gtcaggtcat ccggggctgc tacctcgaca
     1081 aaaaagagcc tgtatccgaa tggaaagcca tccaccggga agtcagccag atcaagaaag
     1141 cgctcagctc gcttgcggtc caatgctttc atctggaatc cgaccacatg gatctgcgga
     1201 tcacgccggg tgaacaacgc aaatgggctg gcgtttccgg ccataacgtg cccagctttg
     1261 aagtgtttgt ctccccggac tggcggggaa ccgagggcgt gtattacgcc aacctcccct
     1321 cctttcgcag cggcaattat gtaaaagatg tccgtatttc gtttgccaaa ggaaaggccg
     1381 tcgccgtcga ggcggcacag ggcgaggcct ttgtgaaaaa acagctcgcc atggatcccg
     1441 gagcctgccg gcttggagag ttctctctta ccgaccggcg cttttcccga atcgaccgct
     1501 ttatggcgga aacgcttttt gacgaaaact tcggcgggaa ggaaggcaac tgccatgtcg
     1561 cactgggggc ctcctacagc gacacgtaca gcggcgaccc ggcgcgtctg accccggcgc
     1621 tcaagaaaaa actgggattc aacgattccg ccctgcactg ggatctggtc aacacggagc
     1681 ccaaaaccgt ctcggcgcat ctcaaaaacg gcaagaaaat catgatttac gaccggggca
     1741 tttttacaat ttaaaccggg gaggctgtct tcatatgaaa gttgatttct attgctggca
     1801 aaagtgaacc acctgccgga aagcgaggga gtttctctcg caaaagaagg tcgatgtaaa
     1861 cgagcgtgat tttttcaaaa aaccctttac cgccgacgag atcagagcgc tgctgcgcgg
     1921 aagccggcct tcggaaatgt ttaatttccg aagccccagt ttcaaaaaac tgtctctgga
     1981 cccggaaagc cttcaggaca aggatatggt tgaacggatg cttcaggagc cgcggctcat
     2041 tcgccgtccg gtcgtgaaaa tcggccgaaa agtttacttc ggcgcttcgg ccgatgtttt
     2101 ggcggacatc ctgaaatcct gaaacggttt acgaaaagag caaggaaaat ccattgtaaa
     2161 ggaaagatcc gatgaaatat ctcacggcga ttgaagcgag gcaatttgcg gaaaaatggc
     2221 tgccggcgtg gacgggaaac catcccgaat tgctggctgg cttctactcg gaagacgctt
     2281 tttacatgga tccggccgtt ccccggggcg tcaggggaaa aaataatctg ctggcttact
     2341 tccggaagct gctggccgtc aatccgaact gggtctggac gcaaatcgaa ggcattccgc
     2401 tggaagacgg atttctgaac aagtggctgg ccaaaatacc ggttggctcc gtcaccctgg
     2461 aagtggtcgg tgtttgcttt gtccaactgg acgatgcggg gaaaatccgc cgcaacgaag
     2521 tttacttcga ccgctcggct cttctttccg aaatggcaaa actaaaaaga agcgcgccgg
     2581 ccggtccttg agccgaatcc gtgcattgaa ccgcaaacaa catcctgatc atggagaaaa
     2641 atgatggaat gggaacgttt gatccgggaa gcacgggaat gcttgcaagt atctcccgaa
     2701 gcaaccgctc gctatatcaa ggcaaaggac atcctgcttg aggaggtcaa cgcccgaatg
     2761 accgcccatc ccgggatttc caacctgacc ggcggcaatc ccatgagcat gatgcgggac
     2821 aatcacagca atcacctcga tttcatgtcc accgtctttg aattcaacag ctttgagctg
     2881 ctggtcaaaa ccgtcccctg ggtataccgc tcctaccact cgcacggctt ttcgtttgac
     2941 tactttccac tggagctgga agcctggaaa aaggcagtca gccaatttct cagccccgaa
     3001 gcagccgggg aaatcaacgc cgtttacgac tggatgctga aaaaccatga acggatgatt
     3061 gaactttccg cgttcatccc cgaccagcgg gagacctatc cggaattgaa ggatgagcgc
     3121 agaagattcg gcggcggtct tctggcggcg gactttccgc tttgcatgaa aattgccgac
     3181 tccgttctgg agcgggaaaa cgggcaggaa gccctttacc tggggctgat ccagccggtc
     3241 atgtatgaaa tcggcagact gtgggaacag gataagatca gcgccgccga agagcacatg
     3301 gccacgtcca tggtgggaag aatcctggcc ggtctctatg ccaggtttcc ggttccgccg
     3361 gcaaaccggg gcaaggccgt ggtcacctcc gcacccaacg agtttcacga actcggcgca
     3421 agaattctgg ccgatatgct ggaagcggac ggatgggaca ttttcttcct gggcgcgaac
     3481 acgcctgccg aagagctcat caaactggtc aaaaaagccg atccgcgttt cctggcgatc
     3541 tcggcaacca tgccgttcaa catggaccgg gtcgcggcca tgatttcagg catccgaaac
     3601 gatcccgccc tgaactccgt caagattctg gtcggcgggg ccgctttcaa cacggaccac
     3661 acgctatggc ggcgaatcgg cgccgacgcc tgggcggaag atcccccgtc cgccatccgg
     3721 caggttcagt catggtaaga gacagcggcg cgccaaccga tctggaaagt ctgctgcagg
     3781 gaaatcccga gaaggtcacc cggtatctcc tgtcttccgg caggatggcc atgatccttc
     3841 tggaccggaa gggcgtcatt ctggattgca acgccttctt cctggaaagc gtcggattat
     3901 cggaaaaacc cgtcggcaaa tccatccagg cttttcttcg ggaaggcctc caagccggat
     3961 gggaatgcat ccgcggagat tgccggcccg tgaaactgac gtttacattg aacggaccgc
     4021 tggaatattt tctgtcgggc cacatcctga atgtccagga gcattatctc cttttcgcgc
     4081 acaccgtccg cctgaccgat catgaaatgg tcgtcaaact gtcccggctc acggatgaac
     4141 tgactgatct gacccggcaa ctcaacaaaa aaaaccggga gctggaagcc gccaacgaaa
     4201 ccatcacccg gctgatgaac accgatccct taaccgggct caccaaccgg cgtcagctca
     4261 aggaaaggat ggacgcggaa ctgtccaaag ccagacgcca cggccacccg ctttccgcgc
     4321 tcatgacgga catcgaccat ttcaaatcca tcaacgacac ctacggacac gccgcgggcg
     4381 acgcagtgct ggtcggggta gcctccattc tgcggcagac gtgccggaaa gaagacatcg
     4441 tcgcccgcta cggcggagag gaatttgtcg tcctccttcc cgattcgccc gccgcggcag
     4501 ccctggattg cgcggagcga atccgaaaag ccattgaggg aaacgtgttt ccggaaatcg
     4561 accggaaggt aaccgtcagt ttgggcgcca ctctctttgc gcccaccgac acggaggaca
     4621 gcttcatcgg gcgggcggac gccgccctgt acgaggccaa gacatccgga cgaaaccggg
     4681 tggtgctgaa atagccttcc aaagcgaagc tgaacggcgg agacgctcat tccctcttcc
     4741 acgggaccag gcgatgcggc ccggaacggc gacgttattg ccgtccggtt caaggtctaa
     4801 tgccaacgcc aactcatttc aaactcgttt gatgatatgc gaaagcgggt gcggcacccg
     4861 tgaggtcgaa ccattcaggc gagccatcag gaatgccgct gaag
//
LOCUS       DAOSIM010000156         4903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_15376_length_4903_cov_7.728548, whole genome shotgun sequence.
ACCESSION   DAOSIM010000156 DAOSIM010000000
VERSION     DAOSIM010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15376_length_4903_cov_7.728548"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..1784)
                     /locus_tag="PLR47_07345"
     CDS             complement(204..1784)
                     /locus_tag="PLR47_07345"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOG12798.1"
                     /translation="MQEKNTDQSSMNADHVRRLTQIGVALSAEKNIDRLLEKILTEAR
                     TITSADGGTLYLMSENERELQFAFIQNESMGVTMGGTGEKITWPPVPMTGADGLPNHA
                     NVSAHVAITREAVNIEDVYDASGFNFDGPRAFDQKTGYRTKSMLVVPMLNHENEIIGV
                     LQMINARNKDTGLVIPFSERARDLAWSLASQAAVALTNNLLILELQTLLEAFIQTIAL
                     AIDEKSPYTGGHVRRVAGLTMQIAEAVNACRDGHYANTRLSEDEMRELRMAAWLHDVG
                     KITTPDHVVDKATKLEKVYDRIGEIKTRFEVLKRDRLLAACGGRENPPGANKAETERE
                     LDELDDERRFLLQMNIGGEFTKDQMIERIRRIAQRQWKSDGEVRPLLTEDEVYNLSIR
                     RGTLNDEEREIINNHAALTHKMLSKLPFPKKLRRISEYAGAHHEKLDGSGYPLGLKGD
                     ELSLQSRIIALADIFEALTAKDRPYKKGKTLGEALKIMEMMVKDHHIDANLYELFIRE
                     KIYMAYAAKELASQQIDV"
     gene            complement(1904..2278)
                     /locus_tag="PLR47_07350"
     CDS             complement(1904..2278)
                     /locus_tag="PLR47_07350"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOG12799.1"
                     /translation="MNIVDSSGWLAYFADEINAKHFMAPLSSPTRLIVPAITLYEVFK
                     VILRESSENEALQAVVAMQKGQVINLNTSLALAAARLSLEHRFPMADSIILATALEFR
                     ATIWTQDADFKDLNKVKYFPKK"
     gene            complement(2275..2481)
                     /locus_tag="PLR47_07355"
     CDS             complement(2275..2481)
                     /locus_tag="PLR47_07355"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOG12800.1"
                     /translation="MQSVTVSPKYQVVIPKEVRDSLKLQPGQKMQVVEYAGRIELIPE
                     RDIKELRGFLKGINTEFKREEDRL"
     gene            2662..3279
                     /locus_tag="PLR47_07360"
     CDS             2662..3279
                     /locus_tag="PLR47_07360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG12801.1"
                     /translation="MLKIDPIKISKIKGFLDEAEAGRLYDLALEASRSAPCLEIGSYC
                     GKSSVYLGCACRENGAIFFSVDHHTGSEEQQPGQEYFDPELLDKETGKIDTFRLFRKT
                     IEDFDLQDTVVPIIGRSDIIGRAWKTPLGLVFVDGSHAYESVLKDYQVWADHVRSGGY
                     LVFHDIFPNPADGGQAPYLVYQQAVASGLFDAAPLFKSLGVLKKK"
     gene            complement(3340..>4903)
                     /locus_tag="PLR47_07365"
     CDS             complement(3340..>4903)
                     /locus_tag="PLR47_07365"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG12802.1"
                     /translation="VIAAPYPLEEFPSPPLGIAYVAAAFEAAGCEVRIFDYIISCYSR
                     EKLAAQLADFQPDAVGAGSVTMNFYDGQRILRDVKSIDPGILTMMGGPHVSFTVEETL
                     QTYPEIDVVFIGEADDTIIEFAPLIRRKDKWLQVRGLAYRDGDGIVHTGRRDFITDVD
                     RIPLPARRLLPISRYRALGYPVSMITGRGCPHGCIFCLGRKMVGAKVRRRNPALVVDE
                     IEQIIGMGFDRMNIADDLFASDTNRVREICRGIRERGLKFTWSAFARVDTVTQEMFDE
                     MASVGCDSVSFGVESGCPEMLKRVRKGITLAQAAEAVRMCKQSGMLAHASFMVGLPGE
                     TKETLQRTDEFARSLDIMYGYHYLAPFPGTTLCEKVERYDLQILTRDWSKYDANDAIV
                     KTSELVPQDIRDFVARYEAEVNGDWQKVVDNYKTGKNTPYDDLRVEGQSRTKITYAIL
                     KEDLVERLGVIDSSLAGGGSKDEAENLLIERMKESISGDPAVVESTVRDFIAKGYLRA
                     DFSDRGCIWRWS"
BASE COUNT         1018 a         1384 c         1277 g         1224 t
ORIGIN      
        1 gtgctttagc acatttatag agcggagcaa tttaccatta aatcaccgct tgcatgggct
       61 tcttaacaca tcagtcaata aaatcaagca aaaatttcct gtttgaaaaa aacccttcag
      121 tctcgcgccc cctgtaaagg gggtcagccc aattcccttt taaaaacctt cagccttcag
      181 tcttcagcct aatcccctta ttttcacaca tcgatctgct gtgaagcgag ctcctttgcg
      241 gcatacgcca tgtaaatttt ttcccgaatg aacagctcgt agaggttggc gtcgatatga
      301 tggtccttga ccatcatttc catgatcttc aaagcttcgc ccagcgtctt gcccttttta
      361 taaggccggt ccttggcggt cagcgcctca aaaatatcgg ccagcgcgat gattctcgac
      421 tgaagggaaa gctcgtcgcc cttgagtccc agcggatagc cgctgccgtc gagtttttca
      481 tgatgcgcgc cggcatactc ggagattcgc cgaagctttt tggggaaagg aagtttcgag
      541 agcatcttat gggtcagggc cgcgtggttg ttgatgattt ccctttcctc gtcattcagc
      601 gtcccgcgcc gaatgctcag gttgtacact tcatcttccg tgagcagcgg ccggacctcc
      661 ccgtcgcttt tccattgtct ttgggcgatc cgccgaatgc gctcgatcat ctgatccttc
      721 gtgaattcgc ccccgatgtt catctgcagc aggaaccggc gctcatcgtc cagttcgtcg
      781 agttctcgtt ccgtctccgc cttattcgca ccgggcgggt tttcgcgtcc gccgcacgcc
      841 gcgagcaggc ggtctctttt cagcacttcg aaccgggttt tgatttcacc gatgcggtca
      901 tacacttttt ccagttttgt cgctttatcc acgacatggt ccggcgttgt gatttttccg
      961 acatcgtgca gccaggcggc catccgcagc tccctcattt cgtcctcgga cagtcgggtg
     1021 tttgcgtaat gcccgtcccg gcaggcattg acggcttcgg caatctgcat ggtgaggccg
     1081 gccacgcgcc ggacgtgccc gccggtgtaa ggggattttt cgtcgatggc cagcgcgatc
     1141 gtttggatga aagcttccag cagggtctgc agttcaagaa tcagcagatt gttggtgagc
     1201 gccaccgccg cctgcgaggc caatgaccat gccagatcgc gggccctttc ggaaaacgga
     1261 atcaccaggc ccgtatcttt gttccgggcg ttgatcatct gcaggacgcc gatgatttca
     1321 ttctcgtgat tgagcatcgg aacgaccagc atggatttcg tgcggtagcc cgtcttctgg
     1381 tcgaacgccc tgggcccgtc aaaattgaac ccggacgcgt cataaacatc ctcgatgttc
     1441 accgcctccc gggtgatcgc cacgtgcgcg gatacgttcg cgtgattggg cagaccgtcg
     1501 gcgccggtca tgggcacggg cggccaggtg attttctcgc ccgtcccccc catggttacg
     1561 cccatggatt cattctggat gaaggcgaac tgaagttcac gctcgttttc cgacatgagg
     1621 tagagcgttc cgccgtccgc ggacgtgatg gtgcgggctt ccgtgagaat tttttccagc
     1681 agacggtcga tattcttttc agcggaaagc gccacgccga tttgcgtcag ccgtcgaacg
     1741 tggtcagcgt tcatcgaact ctggtccgtg tttttttcct gcattaaagc aatgcccgcc
     1801 ctgatcgatg atttttttgt actatctctg aaacaggctt tgctgtcaac cgtgaagttg
     1861 caggtgcgta agcacaggtg ttaaaagcaa atgagttgtg tttttatttt ttcgggaaat
     1921 atttgacctt gtttaaatct ttaaagtccg catcttgagt ccagatagta gccctgaatt
     1981 ccagggctgt tgcaagaata atactgtctg ccatgggaaa ccgatgttcc agactcagtc
     2041 ttgccgcggc cagcgccagt gatgtattta aattgatgac ttgtcctttt tgcatggcaa
     2101 caaccgcctg caatgcctca ttctcactgg attccctcag gatgactttg aatacttcgt
     2161 atagggttat tgcgggaacg ataagcctgg ttggactgct caatggcgcc ataaaatgct
     2221 ttgcattgat ttcatcggca aaataagcaa gccagcctga agaatctaca atattcataa
     2281 tcgatcttcc tcgcgcttga attccgtatt gatgcctttg agaaatccac ggagctcttt
     2341 gatatcacgt tcgggaatca gttcaatcct tccggcgtat tctacgactt gcattttttg
     2401 acccggctga agctttaagg agtctctgac ttctttgggg atgaccacct gatatttcgg
     2461 ggataccgtg acggattgca tgccaatacc tccatcgata tattgttggt attacgatat
     2521 cacaatcgat aggttttggt caatgattcg atgattcaaa ttctgatcca accaaacccc
     2581 ttcgcccttt tcgtcatctt tcaagttgca atttattcgg gggcggttta tgaaaacaaa
     2641 acttccctca aaggattgcc gatgctgaag attgacccga taaaaatatc aaaaatcaaa
     2701 ggatttctgg atgaggcgga agccggtcgc ctctatgatc tggccctcga agcctcccgg
     2761 tccgccccct gcctggaaat cggcagctac tgcggaaaat cctccgtcta tcttgggtgc
     2821 gcctgccggg aaaacggcgc catatttttt tcagtggacc atcataccgg ctcggaggag
     2881 cagcagccgg gccaggaata ttttgatccc gagctgctgg acaaggaaac ggggaaaatc
     2941 gacacgttcc ggctgtttcg caaaacgatt gaagatttcg atcttcagga caccgtcgtc
     3001 ccgattatcg gccggtcgga catcatcgga cgcgcctgga agacgccgct tggccttgtc
     3061 tttgtggacg gaagccacgc ctacgaatcc gttctgaaag actatcaagt ttgggcggat
     3121 cacgtccggt ccggcggcta tctggttttt cacgacattt ttcccaatcc cgccgacggc
     3181 ggccaggcgc cctacctcgt ttaccaacag gcggtcgctt ccggcctctt tgacgcagcg
     3241 cctcttttca aatcgctggg ggttttgaaa aagaaataaa agattgaacg gaaaaaatac
     3301 agtttagcct tataggccgc gcgcagtcat ccgaaggttt cagctccagc gccagatgca
     3361 gcccctgtcg ctgaaatcgg ctctcagata gcctttggcg ataaaatccc gcacggtcga
     3421 ttcaaccact gcgggatcgc cggaaatgga ttccttcatt ctttcgatga gcaggttttc
     3481 cgcctcgtct ttgcttccgc cgccggccag ggatgaatcg atgacgccca ggcgctccac
     3541 gaggtcttcc ttgagaatgg catacgtgat tttcgtccgg ctctgccctt caacccgcaa
     3601 atcgtcgtag ggggtgtttt tccccgtctt gtagttgtcc accacctttt gccagtcgcc
     3661 gttcacctcg gcttcatatc gggcgacaaa atcccgaatg tcctggggca caagctccga
     3721 tgttttcacg atcgcgtcgt tggcatcata tttggaccag tcgcgggtca gaatctgcag
     3781 atcatagcgc tccactttct cgcacagggt cgtgcccgga aaaggcgcaa ggtaatggta
     3841 gccgtacatg atgtccagac tccgggcaaa ttcatccgtc cgctgaagcg tttcttttgt
     3901 ctctcccggc agaccgacca taaaggaggc gtgcgccagc attccggact gcttgcacat
     3961 tcggacggcc tcggccgcct gcgcaagcgt tatgcccttg cgcacccttt tgagcatttc
     4021 cggacagccc gattcgacgc cgaaactgac gctgtcgcag cccacggacg ccatctcatc
     4081 aaacatttcc tgcgtgacgg tgtccacgcg cgcgaaagcg ctccaggtga atttcaggcc
     4141 gcgctcgcga atgccccggc agatttcccg aacgcggttt gtgtccgaag caaaaaggtc
     4201 gtcggcgatg ttcatccggt caaagcccat gccaatgatc tgctcgatct cgtccaccac
     4261 cagggccgga ttgcggcggc ggactttcgc gccgaccatt tttcttccca gacaaaaaat
     4321 gcagccgtgc ggacagccgc ggccggtgat catgctgacg ggatacccca gcgcccggta
     4381 gcgggaaatg ggcagcagac ggcgtgcggg cagcggaatg cggtccacat ccgtgatgaa
     4441 gtccctgcgg ccggtatgga cgatgccgtc gccgtcgcga tacgcgaggc cccgcacctg
     4501 aagccattta tctttccgtc gaatcagcgg cgcgaattcg atgatggtgt cgtccgcctc
     4561 gccgataaac accacgtcga tttccgggta ggtctgcagc gtctcttcca cggtaaagga
     4621 cacatggggt ccgcccatca tggtaaggat gccgggatcg atgcttttca cgtcgcgcag
     4681 aatccgctgc ccgtcgtaaa aattcatcgt gaccgaaccg gcgcccacgg catccggctg
     4741 aaaatcggcc agttgagcag ccagtttttc cctggaataa caagagatga tatagtcgaa
     4801 aatccgcact tcgcagcccg ccgcttcaaa ggccgcggcg acgtaggcaa tgcccagggg
     4861 cggcgagggg aattcttcca gcgggtaagg ggcggcaatc acg
//
LOCUS       DAOSIM010000157         4877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_15471_length_4877_cov_8.053297, whole genome shotgun sequence.
ACCESSION   DAOSIM010000157 DAOSIM010000000
VERSION     DAOSIM010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15471_length_4877_cov_8.053297"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /gene="trxB"
                     /locus_tag="PLR47_07370"
     CDS             complement(<1..930)
                     /gene="trxB"
                     /locus_tag="PLR47_07370"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOG12803.1"
                     /translation="MEKANLPETNYDVIILGGGPAGYTAGIYAARAALKTLLVEGEGT
                     VSQITITDMIENYPGIPDGIGGFELMQLFKKQAQKFGLKILPKDATGVRKIFDAPVIW
                     EATAGGRTFTALSLIAATGAMWRSLGVPGEAQFAGRGVSYCATCDGPFYRNRTVVVVG
                     GGDTAIQEALFLTHFADKVIVIHRRDRLRAAGMLQNRAFAEKKIEFVWNATVEEIAGA
                     EFVTGVKVKDVQTGKVSEIEADGAFIFVGRLPYTDLFKGLVAMDKTGFILTDENLRTD
                     APGIFAAGDCRAKLFRQVVTAAGDGANAVHSAEL"
     gene            1103..3769
                     /locus_tag="PLR47_07375"
     CDS             1103..3769
                     /locus_tag="PLR47_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG12804.1"
                     /translation="MQTQYLMAIDVGGGSGRCLLLDPETGKTLSAKRNWTHPAAPGTS
                     GLGTRIDTDGLLAKLGEASREVLSKAGVKPNRIAGIAVGGMRNTLVALDAQNNVLLAA
                     PNRDARALGEGMMLGAERGSEVHAVSGHWPTPLFLGTRLLWMKNNAPDLFRQTAAALS
                     VTDWLTFILSGKIVAERSQAGETLLFDLQKRLWAFDLIDSLGLARSLFPEPVDSGALI
                     GSLTRQAADLLGLVPGIPVAAGGADTQCGLLGAGAIKDNEAAVIAGTTMPLQLVTNEL
                     VLDKTGRLWSGLHVVPGQYVLESNGLVTGDVIDWFANLLYPDSPDTTLSLFAEAEKSV
                     PGASNVYSTFGTALFDGRTVGFPVGNLTFSHMITGEASQSRRHIARALVEGVAFSVRV
                     NLEQLASVSGRNMNDLRVAGGMSQSPLFSQIVSNVTNSPVLVPRTAEVTSVGAAVLAG
                     VGAGIFADTAAGVEKAASALTRYQPDAAGEKYRNFYAGWRQAFDRRAETDAHVGNLLI
                     NSLFEPSPAAGRAADPSFSPNIFITASMDEAAIRELEAIGKATCAQWREKQKLYDGGP
                     ELAAALAGAQIFITEMDVVDFPAIRDARDLKAIVTCRGNAVNVDLAAATAYGIPVINT
                     PGRNADAVADLTVALMVMLSRKMPGSFDFLKHGQIQSGDMAKMGEAYLKYQGKELWRK
                     TVGLIGLGNVGACVAARLARFGTNAVFYDPMVAAGEGSLLGAKKASLDELLAISDIVS
                     LHAPAIEATKGMMNRKAFDQMKPGAFFVNTARASLVDEAALLEALTSGRIAGAALDVF
                     RSEPPASDDPIVSHPNVIATPHLGGNTIETAAHQGAIAVDQIRMLLAGKKPEYLLNPD
                     VLDVFSWTAPRPEPDANTLEALAKNKRPSMTS"
     gene            3791..4468
                     /locus_tag="PLR47_07380"
     CDS             3791..4468
                     /locus_tag="PLR47_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOG12805.1"
                     /translation="MGLYDSYQKEVLEASLWLLKHGYFGSLRGTGGNVSARIAGHDGM
                     AITPSSVKYSDLTPADICIVDFTGAVVEGKYAPSIEAGMHAAIYRERPDVNAVVHTHQ
                     LYGSIFAALGRPIPVLFDEVAFALGESVEVAPYALSGSPELAANVAKTLVNRANACII
                     QNHGILALGKNMDKALLAAELLEKIAHIYCLALSTGKPVTLLPDATREMARSLRDYDV
                     EQARKKS"
     gene            complement(4528..>4877)
                     /locus_tag="PLR47_07385"
     CDS             complement(4528..>4877)
                     /locus_tag="PLR47_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOG12806.1"
                     /translation="VFNAMLSSICTAAMTLVTSGVASIEDVDRAWMGVMRMPIGPFGM
                     MDQMGLKTVWHVNDYWARNPQEIPAGQIQANADFLKEYVDRGELGARTRKGFYSYPNP
                     AFARPDFLAGEKK"
BASE COUNT          964 a         1533 c         1449 g          931 t
ORIGIN      
        1 cagctccgcg ctgtgcacgg cgttggcgcc gtcgcccgcg gcggtgacca cctggcggaa
       61 aagcttggcg cggcaatccc ccgccgcgaa aatgccgggg gcgtcggtcc gcagattttc
      121 atcggtcagg ataaagcccg ttttgtccat cgccaccagg cctttgaaca ggtccgtgta
      181 gggcaggcgg ccgacaaaaa taaacgcgcc gtcagcctcg atttcactga cctttcccgt
      241 ctgaacgtct tttaccttca cgcccgttac gaattcggcg ccggcaattt cctcgaccgt
      301 cgcattccag acaaattcga tcttcttttc ggcaaaggca cggttttgga gcattcccgc
      361 cgcgcgcaaa cggtcgcgcc ggtgaatcac gatgactttg tcggcaaagt gcgtcagaaa
      421 cagcgcttcc tgaatggcgg tatcgccgcc gcccaccacg accaccgtgc ggttgcggta
      481 aaaaggaccg tcgcaggtgg cgcagtacga aacgcctctt cccgcgaact gcgcttctcc
      541 cggaacgccc aggctccgcc acatcgcgcc ggtcgcggca atcaggctca aagctgtaaa
      601 tgtccggccg ccggctgtcg cttcccagat caccggcgca tcgaaaattt tccggacgcc
      661 ggtcgcgtct ttgggcaaaa tcttcaggcc gaatttctgc gcctgcttct tgaaaagctg
      721 catcagctcg aacccgccga tgccgtcggg aatgcctgga taattttcga tcatgtcggt
      781 gatggtaatc tggctgaccg tcccctcccc ttcgaccagc agcgttttca gcgcggcgcg
      841 cgcggcatag atgccggccg tgtagccggc gggcccgccg ccgagaatga tcacatcgta
      901 gttggtttcc ggcaggtttg ccttttccat ggaaaatctc ctgaagcttt gatgcccgac
      961 tttaatcagg aaggggctct tttgtaaagg ccaaattgag ggggcggcag gacggcgctt
     1021 tatggcacaa gagggcaaaa aggcttgact tgtccaaacc gcctttctat acttcgccaa
     1081 gcaatccaag gagcgtcaac tcatgcaaac gcaatatctg atggccattg atgtgggggg
     1141 aggctccggc cgctgccttc tgctggaccc ggaaacgggc aaaaccttat cggcaaagcg
     1201 aaactggacg catcccgccg cgcccggcac cagcggactg ggcacccgaa tcgacacgga
     1261 cggcctgctc gcaaaactgg gcgaagcctc ccgcgaagtt ctttccaagg ccggagtgaa
     1321 gccgaaccgg atcgcgggca tcgccgtcgg cggcatgcgc aacaccctcg tggccctgga
     1381 tgcgcaaaac aacgtcctgc tggccgcgcc caaccgggac gcgcgcgccc tgggcgaggg
     1441 catgatgctg ggggcggaac ggggaagcga agtccatgcc gtctccgggc actggcctac
     1501 tccgctgttt ctcggaacgc gccttctctg gatgaaaaac aacgctcccg atctctttcg
     1561 gcagacggca gcggcgctgt ccgtcacgga ctggctgacc tttatcctgt cgggaaaaat
     1621 cgtcgccgaa cgttcccagg cgggggaaac gcttcttttc gatctgcaaa agcgcctctg
     1681 ggcctttgat ctcatcgatt cgctggggct ggcacggtcg ctctttccgg agccggtgga
     1741 ttccggagcg ctcatcggca gcctcacccg gcaggcggcc gacctgctgg ggctggtccc
     1801 cggcatcccc gtggccgcgg gcggcgccga cacccagtgc ggcctgctcg gtgcgggcgc
     1861 cataaaagac aacgaagcgg ccgtcatcgc cgggaccacc atgcccctgc aactggtgac
     1921 gaatgagctg gtcctggaca aaaccggacg cctctggagc ggcctgcatg tcgttccggg
     1981 acagtacgtt ctggaaagca acggcctggt cacgggcgac gtcatcgact ggtttgccaa
     2041 tcttctctat ccggattctc cagatacgac tttgagcctc tttgcggagg cggaaaaatc
     2101 cgttccgggc gcttccaatg tctattccac gttcgggacc gccctgttcg acggaagaac
     2161 cgtgggcttc cccgtgggca acctgacctt ttcccacatg atcacggggg aagcttccca
     2221 aagccgcagg cacatcgccc gcgccctggt agaaggggtc gctttttccg tccgcgtcaa
     2281 cctcgaacag ctcgcctccg tttccggaag gaacatgaat gacctgcggg tcgccggagg
     2341 catgtcgcaa agcccgctgt tttcgcaaat cgtctccaac gtgacgaatt ctcccgtcct
     2401 cgtcccgcgc accgcggaag tgacgtccgt cggagcggcc gttctggcgg gcgtcggagc
     2461 gggcatcttt gccgatacgg cggcaggggt tgaaaaagcg gcctccgccc tgacccggta
     2521 tcagccggac gcggcggggg aaaaataccg gaatttttat gcgggctggc ggcaggcttt
     2581 cgaccggcga gccgaaacgg acgcccacgt cggaaatctg ctgatcaatt ccctgttcga
     2641 gccgtcaccc gccgcgggca gggcggcgga cccgtctttt tcgccgaata tcttcatcac
     2701 cgcttcgatg gatgaagccg cgattcgcga actcgaagcg atcggaaagg caacctgcgc
     2761 ccaatggagg gaaaagcaga agctctatga cggaggcccg gaactggccg cggcactcgc
     2821 cggcgcgcaa atcttcatca ccgaaatgga cgtggtggat tttccggcca ttcgcgatgc
     2881 tcgggatctg aaggccatcg tcacctgccg cggaaacgcc gtcaacgtcg atctcgcggc
     2941 cgcgaccgct tacggcattc ccgtcatcaa cacgccgggc cgaaacgccg atgccgtcgc
     3001 ggacctgacc gtcgcgctga tggtgatgct gtcgcgcaaa atgcccgggt cattcgactt
     3061 tctgaaacac ggacagatcc aatcgggcga catggcaaaa atgggagaag cctatctgaa
     3121 ataccagggg aaggagctct ggagaaaaac cgtcggactg atcgggctgg gcaatgtcgg
     3181 ggcctgcgtc gcggcgcgcc tcgcgcggtt cggaaccaac gctgtttttt acgatccgat
     3241 ggtcgccgcc ggggaaggct ctctgctggg cgcgaaaaaa gcatccctcg atgaactgct
     3301 ggccataagc gacatcgtca gtcttcacgc tccggccatc gaggcaacga agggaatgat
     3361 gaaccggaaa gcctttgatc aaatgaagcc cggagccttc ttcgtcaata ccgcccgcgc
     3421 gtcccttgtg gacgaagcag cccttctgga ggcgctgaca tcgggccgca tcgccggagc
     3481 cgcgctggat gttttccgaa gcgagcctcc cgcatcggac gacccgatcg tctcccaccc
     3541 caacgtgatc gccacgccgc acctgggagg caacaccatc gaaaccgctg cgcatcaggg
     3601 ggctattgcc gtggaccaga ttcgaatgct tctggcgggg aaaaaaccgg agtatctgct
     3661 caatccggat gttctggatg ttttttcctg gacggctccg cgccccgaac cggacgccaa
     3721 caccctggag gcgctggcca aaaacaaacg cccttcaatg acgtcgtaga aaccggaaag
     3781 gagttcaacc atgggcctct atgattctta tcaaaaggaa gtgctcgagg cgagcctctg
     3841 gcttttgaag cacggctact tcggctctct caggggcacc gggggaaacg tgtcggcgag
     3901 aatcgccggc catgacggca tggccatcac gccgtcttcc gtcaaatatt cggacctcac
     3961 gccggccgac atctgcatcg ttgatttcac gggcgccgtc gtcgaaggga aatatgcccc
     4021 ttcgatcgag gccggaatgc atgcggccat ctatcgcgaa cggccggatg tgaacgccgt
     4081 ggtgcacacg catcagctct acggcagcat ctttgccgcg ctgggccggc ccattcccgt
     4141 tttgtttgac gaagtcgcct ttgcgctggg cgagtcggtg gaggtcgccc cttacgccct
     4201 ttcgggaagc ccggaactgg cggccaatgt ggcaaagacg cttgtgaacc gcgccaacgc
     4261 ctgcatcatt cagaaccacg gaatcctcgc gctgggaaaa aacatggaca aggcgctcct
     4321 ggccgcggag cttttggaaa agatcgctca catttactgc ctggctctgt ccacggggaa
     4381 gccggtcact ctccttcccg atgccacacg ggaaatggcg cgcagcctgc gggattatga
     4441 cgtggagcag gcccggaaaa aatcgtagcc ttttcccgac ccgaaaaatc gtttctcctc
     4501 cggggcgcga aacggagaaa gccgccgtta ttttttctcc cccgcgagaa aatccggccg
     4561 ggcaaaagcc gggttgggat aggaataaaa tcccttcctc gttctggcgc cgagttcgcc
     4621 gcggtccacg tactctttca gaaagtccgc gtttgcctga atctggccgg ccggaatttc
     4681 ctggggattg cgcgcccagt agtcattgac gtgccagacc gttttcaggc ccatctggtc
     4741 catcatgccg aaggggccga tcggcatgcg catcactccc atccaggccc ggtccacatc
     4801 ctcgatgctg gccacgccgg aagtcacgag cgtcatggcg gccgtgcaga tgctggagag
     4861 catggcgttg aacacgt
//
LOCUS       DAOSIM010000158         4834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_15630_length_4834_cov_9.139987, whole genome shotgun sequence.
ACCESSION   DAOSIM010000158 DAOSIM010000000
VERSION     DAOSIM010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15630_length_4834_cov_9.139987"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1043)
                     /locus_tag="PLR47_07390"
     CDS             complement(48..1043)
                     /locus_tag="PLR47_07390"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOG12807.1"
                     /translation="MMKWLKANWFYLAVLAAALVLPLFITNRYYSQVITMSLLFAIGA
                     LSLNLIMGFTGQASLAHGGFFAIGAYGVAILTYTLGWNFWLALPASALLAGAVGFVVG
                     LPALRTRGSYFAITTLCLGEIIYLIAGNWMELTGGHNGIVGIPVPSPIMIPGMGQLNF
                     ESQIPQYYLVLFFLLATLFVLYRLTKSLRGLTFLATAINEDLAEAVGINTFRTKLLSF
                     VVANFFAGLAGGIYASLIGSISPSVASVAMTFNFLMYVLLGGITTLAGPIIGAFLIPV
                     LLEYLQFLQDYQMILFGFLLIIVIIYFPTGFMGLYKKVSDKMKKSKVGAKRAAER"
     gene            complement(1040..1909)
                     /locus_tag="PLR47_07395"
     CDS             complement(1040..1909)
                     /locus_tag="PLR47_07395"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOG12808.1"
                     /translation="MELFLQQVFNGIMLGSTYAIVALGLTMVFGILHVPNFAHGQMYM
                     LGAYVCFFLITMFGFSYWPALAVSAVALALGGVILEVLVFKPVRNAGGITPFIAALGA
                     LIILENAVVVLWGPAGHRIPNPYPGIIDAFGITMSTQRLLVIGVAFLLIVLLQIFIKK
                     TTLGSTIEAVAQNKEGATLVGINVNLVSAMTFAISTGLAAIAACLVAPIFMISPSMGS
                     LVGMKAFVIVILGGLGSIPGAIVGGIILGLIEAIGGGYLSAAYKDVFGFGALVLILAI
                     RPTGLFGKEIKGR"
     gene            2286..3047
                     /locus_tag="PLR47_07400"
     CDS             2286..3047
                     /locus_tag="PLR47_07400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG12809.1"
                     /translation="MIRSAVLVSIGVAITAFFSFWAVTYSFFDNAENNVHKVARIWSR
                     ILLAISKTKVQVIGRENIAPGRPQVLMANHQSDFDILIALAFVPGQFRWIAKKELFAV
                     PVFGQAMRSAGYIEIDRQNHEKALRSLDLAALRIREGKSVMTFPEGTRSRDGEIKAFK
                     QGTFYLAIQAGAPIVPISIVGSGKIMPKRSLKIKSGKIKLVIDKPIPAVGYTLESRHE
                     LIQRVRQVIISNYNAYRDAGTASLKDVQVDAKATL"
     gene            3047..>4834
                     /locus_tag="PLR47_07405"
     CDS             3047..>4834
                     /locus_tag="PLR47_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOG12810.1"
                     /translation="MQEDFSHPPSARFREIKGVVCLALSLFLFLCLFSFSPDDPSPMK
                     FIGDPSSTRNWTGIVGSHAAGWMIFLLGLASFLLPAASLALAFQFFRRPDFGLKIQRV
                     TGFLFLTLACAALFDALIPGGVTVYKTTFPSGGVIGAGLVRFLQGYFNPVGTFILLIV
                     WMMVALFFTVEFSLVSATERFSQSVRIGLSAAWGRIVSFCSGWWTRLKTEKNPPPVIE
                     DAGAKAPPRKAKAKKAEQTHFDFSRMKPDGDFQLPPFTLLSEPPQKDTRVKRDHLITN
                     SRILEKKLADFGVEGRVVEVLPGPVITMYELEPASGVKINRITSLTDDLALALMAPSI
                     RILAPIPGKSVIGIEIPNLKREPVFLKDVLDNDAFTESSLRLPIALGVDFVGAPVIAD
                     LARMPHLLIAGTTGSGKSVALNAMICSILFKAPPQDIKFLMVDPKRLELSSYEGIPHL
                     MHPVVVDPKKASQVLRWTVEEMERRYQIISQMGVKSIDAYNEMLVTRGKTRKAAREPK
                     PEPRPDADTEEAAIESDAPFEHVQLPYIVVVIDELADLMMVAPRDVEESLTRLAQMAR
                     AAGIHLILATQRPSVDVITGLIKANFPTRI"
BASE COUNT         1114 a         1436 c         1349 g          935 t
ORIGIN      
        1 tgacatcatt gacggcaatg gtgccgccaa aattttttac cagattttca cctttcagca
       61 gcacgtttgg cccccacttt cgatttcttc attttgtcgc tcactttttt atacaatccc
      121 ataaatcccg tcgggaagta gatgatcacg atgatcagca aaaatccgaa caaaatcatc
      181 tgataatcct gcaggaactg cagatattcc aggagaaccg gaatcaaaaa ggcgccgatg
      241 atcggaccgg ccagtgtcgt gatgccgccg aggagcacat acatcaggaa gttgaacgtc
      301 atggcgactg aagcgaccga gggagaaatg ctgccgatga ggctggcgta aatgccgccc
      361 gcgagccccg cgaagaaatt ggccacgacg aaagacagca gcttggtgcg gaaggtgttg
      421 atccccacgg cttccgccag gtcctcgttg atggccgtag ccagaaacgt caagccgcgc
      481 agggatttcg tcaggcgata caggacaaaa agggtcgcca gcaggaaaaa caggacgagg
      541 taatactggg gaatctggga ttcaaaattc agctgcccca tgccgggaat catgatcggg
      601 ctgggaacgg gaatgcccac gatgccgttg tggccccccg tcagttccat ccagttgcct
      661 gcgatcaggt agatgatttc gcccaggcac agcgtggtga tcgcgaagta ggacccccgg
      721 gtgcgcagcg ccggcagacc gaccacaaat ccgaccgcac cggccagaag cgccgacgcg
      781 ggaagggcca gccagaagtt ccagcccagc gtataagtga gaatcgcgac gccgtatgcg
      841 ccgatcgcaa aaaacccgcc gtgcgccagc gaggcctgtc cggtaaagcc cataatcaga
      901 ttcaggctca atgcgccgat cgcaaaaagc aggctcatcg taatgacctg actgtaatac
      961 cggttcgtta tgaaaagcgg aagaaccagg gctgccgcca gaaccgccag ataaaaccaa
     1021 tttgccttca gccacttcat catctgccct taatctcctt cccgaacagg ccggtcggcc
     1081 tgatcgcgag gatcagcacg agagcgccga aaccgaacac atccttgtat gccgccgaca
     1141 aatacccgcc gccgatggcc tcgatcaatc ccagaatgat gcctcccacg atcgctcccg
     1201 gaatgcttcc cagtccgccg agaataacga tgacaaaggc tttcatgccg acgagcgagc
     1261 ccatgctggg agaaatcatg aaaatggggg cgaccaggca ggcggcgatg gcggccagcc
     1321 cggtggagat ggcaaaagtc atggcggata ccaggttgac gttgatgccg accagcgtgg
     1381 cgccttcctt gttctgggca accgcctcga tggtgctgcc cagcgttgtt tttttaatga
     1441 agatctgcag gagaacaatc agcaggaagg caactccgat cacgagcagg cgctgcgtgg
     1501 acatcgtgat gccgaaggcg tcgatgatgc cgggatacgg attcggaatc cggtggcctg
     1561 ccggtcccca aagcacgacg accgcgtttt cgaggatgat cagcgcgccc agggcggcaa
     1621 tgaacggggt gatgcccccg gcgttgcgga ccggcttgaa gaccaggacc tccagaatga
     1681 cgccccccag tgcaagcgcg acggccgaca ccgccagcgc gggccagtag gaaaaaccga
     1741 acatggtaat taaaaagaag cagacatagg cgcccagcat gtacatctgg ccgtgcgcaa
     1801 agttcggcac atgcagaatg ccgaaaacca tcgtcaggcc cagggcgacg atggcatacg
     1861 tgctccccaa catgattccg ttaaaaacct gctgcagaaa tagttccatg gatactccat
     1921 tatccgaatg acgcagcccc gttccctgcc tttcaacaca taaagtccgg gcggcgttcc
     1981 attggtgttg ctcttgaatg tcttttgctt aggcaggctt cttaaatgag cggcaaccat
     2041 ttgtcaagcg gaaaatcctt tttttcggca tttttgacaa atttatcaaa agccgcaatc
     2101 ccttatcctg attgaaacac gacgtgaccg ggagtcatcg gcaggccgca aatgatccgt
     2161 ccgaacgagg gcggatgcct cgggagaaaa acgcgattca ctgttgactt caacaggttt
     2221 atcaagtaga aaaaccttga aaaaacattc ttggcgcctc ggcattcgag caggaaaaaa
     2281 taccgatgat ccgttcagcc gttttagtct ccataggcgt ggccatcacc gctttctttt
     2341 cattctgggc ggtgacctat tccttttttg acaatgcgga aaacaacgtc cacaaggtgg
     2401 cccggatctg gagccggatt ttgctggcga tcagcaaaac gaaagtgcag gtcatcggcc
     2461 gggagaacat cgcgcccggc agaccgcagg tgctgatggc caaccaccag agcgattttg
     2521 acattctcat cgcgctggcc ttcgttccgg gacagtttcg ctggatcgcc aaaaaagagc
     2581 tgtttgccgt tcccgtcttc ggccaggcca tgagaagcgc gggctacatt gaaatcgacc
     2641 ggcagaatca cgaaaaagcc ctgcggagcc tggacctggc ggccctgcgg atccgcgaag
     2701 gcaagtcggt catgaccttc cccgaaggca cgagaagccg cgacggggaa atcaaggcct
     2761 tcaagcaggg cactttttac ctggccattc aggcgggcgc gccgatcgtt ccgatcagca
     2821 tcgtcgggag cggcaaaatc atgccgaagc gttcgctgaa aatcaaatcc gggaaaatca
     2881 agctggtcat cgacaagccg attcccgcgg tcggatacac gctggaaagc cggcatgaac
     2941 tgattcaaag ggtccggcag gtcattatca gcaattacaa cgcctaccgc gatgccggta
     3001 cggccagcct caaagacgtc caggtggacg cgaaagcgac gctctgatgc aggaagattt
     3061 cagccatcct ccgtctgcaa gatttcggga aatcaagggc gtcgtctgcc tcgcgctgtc
     3121 gctgtttctc ttcctgtgcc tgttttcctt ttcgccggac gacccctccc ccatgaaatt
     3181 catcggcgac ccctcgtcaa cgagaaactg gacgggcatc gtcggctccc atgcggccgg
     3241 ctggatgatt tttcttctgg gccttgcctc tttcctgctg cccgccgctt ccctggcgct
     3301 ggcatttcaa tttttccggc gtccggattt cggcctgaaa atccagcgcg taacaggctt
     3361 cctgtttctg acgcttgcct gcgccgctct tttcgacgcc ctgatcccag gcggcgtcac
     3421 ggtgtacaaa acaaccttcc cgtcgggagg cgttatcggc gccggccttg tccggtttct
     3481 gcagggctac ttcaatccgg tgggaacctt tattcttctg atcgtctgga tgatggtcgc
     3541 gctgtttttc accgtcgaat tctcgctggt gtcggcaacc gaaagattct cgcagtccgt
     3601 aaggatcggg ctttccgccg catggggccg gatcgtttcc ttctgtagcg gctggtggac
     3661 gcggctgaaa accgaaaaga atccgccgcc cgtcatcgag gacgccgggg caaaagcccc
     3721 gcccagaaaa gcaaaagcga aaaaagccga gcagacgcat tttgattttt cccgaatgaa
     3781 gcccgacggc gactttcagc tgccgccgtt tacgctgctc agcgagccgc cgcaaaaaga
     3841 cacccgcgtc aaacgcgacc acctgatcac caactcgcgc attctggaaa agaagctggc
     3901 cgacttcggc gtcgaagggc gcgtggtgga ggttctgccc ggcccggtca tcaccatgta
     3961 cgagctggag ccggcttcgg gcgtcaagat caaccgcatc accagcctga ccgacgacct
     4021 ggcgctggcg ctcatggcgc ccagcatccg cattctggcg ccgattcccg gcaagtccgt
     4081 gatcggcatt gaaattccca atctcaaaag agagccggtg ttcctcaagg acgtgctgga
     4141 caacgacgcc tttaccgaat cctccctgcg tctgcccatc gcgctgggcg tggatttcgt
     4201 cggcgcgccg gttattgccg acctggccag aatgccgcac ctgctcattg ccgggaccac
     4261 cggctccggc aaaagcgtcg cgctcaacgc catgatctgc agcattcttt tcaaggcgcc
     4321 gccccaagac atcaagtttc tgatggtcga tcccaagcgg ctggagctgt cttcctacga
     4381 aggcattccg catctgatgc acccggtcgt cgtggacccg aaaaaggcct cgcaggtgct
     4441 gcgctggacg gtggaggaaa tggagcgccg ctatcagatc atcagccaga tgggcgtcaa
     4501 aagcatcgac gcctacaatg aaatgctcgt cacccgcggc aaaaccagga aggcggccag
     4561 ggagccgaaa cccgaaccac ggcccgacgc ggacacggag gaggccgcaa tcgaaagcga
     4621 cgctccgttt gagcatgtcc agctgcccta catcgtcgtc gtgatcgacg agctggccga
     4681 tctgatgatg gtcgcgcccc gggacgtgga agaatcgctg acgaggctgg cgcagatggc
     4741 gcgggcggcc ggcatccacc tgatcctcgc cacgcagcgc ccctcggtgg acgtcatcac
     4801 cggactgatc aaggccaatt tccccacgcg catt
//
LOCUS       DAOSIM010000159         4781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_15850_length_4781_cov_7.048879, whole genome shotgun sequence.
ACCESSION   DAOSIM010000159 DAOSIM010000000
VERSION     DAOSIM010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15850_length_4781_cov_7.048879"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PLR47_07410"
     CDS             complement(<1..321)
                     /locus_tag="PLR47_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12811.1"
                     /translation="MTAATQQALSGLRDLSTIQWYVIPLLAIVIYIYAREIREARKTA
                     DWNAVLAGLTIFGVDFFNETWNGWVLHFTGRSAFWTTPGETALRTMVGWNIEIMFMFL
                     LVGII"
     gene            complement(351..1817)
                     /locus_tag="PLR47_07415"
     CDS             complement(351..1817)
                     /locus_tag="PLR47_07415"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOG12812.1"
                     /translation="MRHWAAPDQTRIVFDLSDEPAYRFEIQQDRIILEFSDASFSPSL
                     PSEKIVRKPGVAKILFIRGEDQLCTVQIHLKDHLRAEVFPLKKFMDKPDRVVVDVFLA
                     EAEKAPAVVKRKAVSAPRRVIVIDPGHGGEDPGAIGKNGTYEKHVVLAISREIKKTID
                     KMPGYRAVLTRDGDYYVSFSKRLGVARKHQASLFISVHADAARNREAQGSSVYCLSTG
                     AASNEAAKLLARNENLSDIIGGVPGGEGNNQSDEIILNMFQTNTINLSKTYAADLLDQ
                     IGRVQMLKYPTFHEAPFRVLKLPDTPAVLLETAYLSNPQEEKLFKTQSFRKNIARAVA
                     SSVDRYFAGSNAVANAVAESGGEDAGESRDAKQPKKAAGAAGAEGRMKTAAYTVVRGD
                     TMQKIAQKHETDLAALLTLNNMKIHDPLFAGRKIRVPAAGAQTDGKRRLKKYTVQKGD
                     TLFSLAKSRSTTMEELRRINQMTDADVLRVGQSIRLPE"
     gene            2153..3712
                     /locus_tag="PLR47_07420"
     CDS             2153..3712
                     /locus_tag="PLR47_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOG12813.1"
                     /translation="MPENRFKEALLNPSKFPVTWELVPGRGAKEAAQEKCLAQARQAA
                     GGDKVCALTLTDNPGGTPAMSADFMGAEILRLGIEPLVHFTGKDKNRNQSESQLYALD
                     RAGVRNLLVMSGDYPVSGFQGRPAPVFDLDPIHTLQLISNMNRGLAYPGIKGAVTHQP
                     SDFFPGAVVSPFKATESEQVGQYDKLKKKIDAGAKFIVTQLGYDARKFHEALLFIKQN
                     YPQIPLVGNIYILPFGAAKMMNRNDLPGSVVTDQLLADIDRERSDPDKGEGARILRAA
                     RMYAILKGMGYSGVHIGGHNIRYEQVEEIIRQGEALVPQWTNLVRYFHYPIDNGFYFF
                     EADPATGLNRDTPTDRQCRPPDAKVGWMYGFSRFFHRLMYEPGKNLYGAMKALCRTVQ
                     GTRLEGIFHKLEHLTKVVLFDCRDCGDCALPDAAYLCPMSQCPKNQRNGPCGGSFQGW
                     CEVYPQKRLCIYVRAYARLKKHGEESRLAGNIVPPCNWDLYQTSSWINYYLGKDHTSA
                     KQGGAGSPGAP"
     gene            3791..3867
                     /locus_tag="PLR47_07425"
     tRNA            3791..3867
                     /locus_tag="PLR47_07425"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3825..3827,aa:Arg,seq:ccg)
     gene            complement(4077..>4781)
                     /locus_tag="PLR47_07430"
     CDS             complement(4077..>4781)
                     /locus_tag="PLR47_07430"
                     /inference="COORDINATES: protein motif:HMM:NF015214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HOG12814.1"
                     /translation="QVLAYAFASYHRLFGTCKMTGTLELMTGAASLIAEYNGVQNMPH
                     VRKKLAWMAYITETVQMLAKQACLDPVTEFGMDVMMPNRMAINASKWTYASNFHTMCQ
                     HMQDIGGGLTTTVPAFRDWDNPEIKPFIDKYLAAKDGVSTEERLRILRLIKDGTGNHW
                     QVDTIHGEGSMAAQEMFIYGSANWKKFQAAAKRAAHMDGWQDDTTYGKLPLMDQCVQM
                     PPIDETYKSFAPTAKK"
BASE COUNT         1030 a         1377 c         1344 g         1030 t
ORIGIN      
        1 gatgatgccc accagcagaa acatgaacat gatttcaatg ttccagccga ccattgtgcg
       61 cagcgccgtt tcgccgggcg tggtccagaa agcggaccgg cccgtgaaat gaaggaccca
      121 gccgttccag gtctcgttga agaaatcgac gccaaagatg gtcaaaccgg ccagaacggc
      181 gttccagtcc gccgttttcc tggcttccct gatttcccgg gcgtaaatgt aaatcacaat
      241 cgccagaagc ggaatcacat accattgaat agtggaaagg tcgcgaagac cgcttagagc
      301 ctgttgggtt gctgccgtca tgaaagcctc ctttcaaata tctggttgtc ttactccggc
      361 agccgaatgc tctgccccac ccgcaacaca tcggcgtccg tcatctgatt gatgcgccgc
      421 aattcttcca tggtcgtgga acggctcttc gccagcgaaa acagcgtgtc gcctttttgg
      481 actgtgtatt ttttcaggcg tcttttgccg tccgtttgcg ccccggcggc cggaacgcgg
      541 attttccgcc cggcaaaaag aggatcgtgg atcttcatat tgttcagtgt caaaagggcg
      601 gcgaggtcgg tctcgtgctt ctgcgcaatc ttctgcatcg tgtcgccccg cacgaccgtg
      661 tacgcggccg ttttcatcct gccttcggcg cccgccgccc cggcggcctt cttgggctgc
      721 tttgcatccc ggctttcccc cgcgtcttcg ccgccactct cggcaaccgc attcgcaacc
      781 gcattcgatc cggcaaaata gcggtcaacg gacgaagcca ccgccctggc gatgtttttc
      841 ctgaaggatt gcgttttgaa cagcttctct tcctgcggat tggaaaggta ggcggtttcc
      901 agaagcacgg cgggggtatc cggcagcttc agcacacgga agggcgcctc gtgaaacgtg
      961 ggatatttca gcatctgtac gcggccgatt tggtccagca gatcggccgc gtaggtcttg
     1021 gacaggttga tggtgttggt ttgaaacatg ttcaggatga tttcgtcgga ttgattgttg
     1081 ccttcgcccc cgggcacgcc gccgatgatg tcggagaggt tttcattccg ggccagcaat
     1141 ttggcggctt cgttgctggc cgctcccgtg gacagacaat aaacggagct gccctgggcc
     1201 tcacggttgc gggctgcatc cgcgtggaca ctgataaaga ggcttgcctg gtgcttgcgc
     1261 gccacgccga ggcgcttgct gaaggatacg taataatctc cgtcgcgggt caggaccgcc
     1321 cgatacccgg gcatcttgtc gatggttttc ttgatctccc ggctgatggc cagcacaaca
     1381 tgcttttcgt acgtcccgtt tttgccgatg gcgccgggat cttctccgcc gtggccggga
     1441 tcgatcacga tgacccgcct gggggccgag acggccttgc gtttcacaac ggcaggagct
     1501 ttttcagcct ccgccagaaa aacatccacc acgacgcggt ccggcttgtc catgaacttc
     1561 ttcagcggga aaacttccgc gcgcaaatga tctttcagat gaatctgaac cgtgcagagc
     1621 tgatcctccc cgcgtataaa aagaattttg gcgacgcccg gctttcggac gattttttca
     1681 gacggcagag agggagaaaa ggaggcgtcg gagaattcca ggatgatcct gtcctgctga
     1741 atctcaaacc ggtatgcggg ttcgtcgctg agatcgaaaa caatgcgcgt ctgatcgggc
     1801 gccgcccaat gcctcacatt ggatatctcc gttagcgaat accccggagc ggggaaaaat
     1861 gccggcagga aaaaggccag gcaacaccag agccgccagg ccctaagaca cacaaggacg
     1921 gaactctccc gcgcggttcg cggggagccg atggaaatgg agtgccgccc gtccatcccg
     1981 ccgcgggcgg cggaaaatgc gctcgttgcc ggattcatgc cgatttgagt aacaaatttc
     2041 aggggcaact gcaaggaaag gttgtcttca agcgctaaaa acagttgaca agctttttcg
     2101 taaatgcgat tttcagcgcg caaaaacagc gaatccattc ttgaggaaaa gcatgccgga
     2161 aaaccggttc aaagaggccc tgctgaatcc ctccaaattc cccgtgacct gggaactggt
     2221 gccgggcaga ggagcgaaag aagccgcgca ggaaaagtgc ctggcccagg caaggcaggc
     2281 agccggggga gacaaggtct gcgcgctcac gctcacggac aatccgggcg gaaccccggc
     2341 catgtccgcc gacttcatgg gcgccgaaat cctccggctg ggcatcgaac ccctcgtcca
     2401 ttttaccggc aaggacaaaa accgcaacca gtctgaaagc cagctttacg cgctggatcg
     2461 cgcaggcgtc cggaatctcc tcgtcatgag cggcgattat cccgtgtccg gttttcaggg
     2521 aaggcccgcg ccggttttcg acctggatcc cattcatacg ctgcaattga tctcgaatat
     2581 gaaccggggg cttgcctatc ccggcatcaa aggagccgtc acccatcagc cttccgattt
     2641 ttttccgggc gcggtcgtct cccccttcaa ggcgacagaa tccgagcagg tggggcaata
     2701 cgacaagctg aaaaagaaaa tcgacgccgg cgcaaagttc atcgtcacgc aactgggata
     2761 cgacgcacgg aaattccatg aagcgctcct cttcataaag cagaattatc cgcagatccc
     2821 cctggtggga aacatttaca tccttccctt cggcgcggcg aaaatgatga accgcaatga
     2881 cctgccggga tccgtcgtca cggatcaact gcttgcggac atcgatagag agcgaagcga
     2941 cccggacaag ggggaaggcg caagaatcct tcgcgcggca agaatgtacg ccattctgaa
     3001 gggaatgggc tacagcggcg ttcacatcgg cggccacaac attcgatacg aacaggtgga
     3061 agagatcatt cggcagggag aagcgcttgt tccgcagtgg accaatctgg tccggtattt
     3121 tcattatccc attgacaacg ggttttactt ctttgaagcc gatccggcaa ccggactgaa
     3181 ccgggacacc cccaccgatc ggcagtgccg cccgccggat gcgaaagtcg ggtggatgta
     3241 cggattttcc cgcttttttc accggctgat gtatgaaccc ggaaaaaacc tctacggcgc
     3301 gatgaaggcg ctgtgccgga ccgtgcaggg aaccaggctg gaggggattt ttcacaagct
     3361 ggaacattta accaaggtcg tcctgtttga ctgccgcgac tgcggcgact gcgcccttcc
     3421 cgacgcggcc tatctgtgcc ccatgtcgca atgcccgaaa aatcagcgca acggcccctg
     3481 cggcggaagt tttcagggat ggtgcgaggt ctatccccaa aagaggctgt gcatctacgt
     3541 ccgggcttac gcccggttga aaaaacacgg ggaggaatcg cgtctggccg ggaacatcgt
     3601 gccgccctgc aactgggacc tttatcaaac ctcctcctgg atcaattact atctggggaa
     3661 agaccataca tccgcaaaac aaggcggtgc gggatcgccc ggcgctccct gacagtcttc
     3721 tcccctcgtt ttcaggaaaa ggaatttaac cgttgaagat tccgagggga tagtgtagaa
     3781 gcacccgcgt gggcaattag ctcagctgga tagagcgctg gcctccgaag ccataggccg
     3841 agagttcgaa tctctcattg cccaccaagc catcatccga tacaatccaa gaaagtgcac
     3901 gaaacccgcc aggagtagcg ggttttttgc ccttcttcac acaggccaat caagaaagag
     3961 agcttcatcc gcttcccggg gatgcgcgtt gaatgtccca cggttcaatg gatcaaaacg
     4021 gatagcatct tgttcgggaa aacagccgct tttgatggat tgaaaaaccg tattcttcac
     4081 tttttcgccg tcggcgcgaa gcttttatag gtttcgtcga tcgggggcat ttgaacgcac
     4141 tgatccatca ggggcagctt gccgtacgtg gtatcgtcct gccagccgtc catgtgcgct
     4201 gcccgctttg ccgccgcctg gaatttcttc cagttggcgc tgccgtagat aaacatctcc
     4261 tgagccgcca tcgatccttc gccgtgaatc gtatcgacct gccagtgatt tcccgtaccg
     4321 tccttgatca gccgcaatat gcgcagcctc tcttcggtgg aaacgccgtc tttcgccgcc
     4381 agatacttgt cgataaacgg tttgatttcc gggttgtccc aatcccgaaa agcgggaacg
     4441 gtggtggtaa gtccgccgcc gatatcttgc atgtgctggc acatcgtatg gaagttgctc
     4501 gcgtaggtcc actttgaagc gttgatcgcc atgcggttgg gcatcatgac gtccatgccg
     4561 aactctgtta cgggatcgag acaggcctgc ttggcgagca tctggacggt ttccgtgatg
     4621 taggccatcc aggccagttt tttgcggaca tgcggcatat tctggacgcc gttgtattca
     4681 gcgatgagac tggccgcgcc cgtcatcagt tccagcgttc ccgtcatctt gcaggtgccg
     4741 aacagacgat gatagctggc aaacgcatac gccagcacct g
//
LOCUS       DAOSIM010000160         4765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_15916_length_4765_cov_7.604459, whole genome shotgun sequence.
ACCESSION   DAOSIM010000160 DAOSIM010000000
VERSION     DAOSIM010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15916_length_4765_cov_7.604459"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PLR47_07435"
     CDS             complement(<1..771)
                     /locus_tag="PLR47_07435"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOG12815.1"
                     /translation="MKLASRVAKVKPSETLAITAKANALRAQGRDVIGFGAGEPDFDT
                     PDHIKQAAIRAIEAGFTKYTPVGGTDELKDSIIAKLKTDNRLEYKRSQVVVSCGAKHT
                     LYNLAQALFEEGDEVIIPAPYWVSYPDIVVLAGGTPVILNTLEKDGFKMKPEQLKASI
                     TPRTRAVIFNGPSNPTGAAYSPDELKALAAVLADTDIFAVSDDIYEKILYADFPFANI
                     VMADSRLKDRTIVVNGVSKAYAMTGWRIGYAAGPEEIIG"
     gene            complement(775..1290)
                     /gene="coaD"
                     /locus_tag="PLR47_07440"
     CDS             complement(775..1290)
                     /gene="coaD"
                     /locus_tag="PLR47_07440"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOG12816.1"
                     /translation="MITEKTGGRVAIYPGSFDPITNGHVDIIKRGSRIFDKIIVLVAY
                     NPRKSFLFTVEERCRLIREVFSDCEYVQVDSHDGLLIDYLQSSGASIIMRGMRALSDF
                     EYEFQMALMNRRQTKNVETVFLMSGFRWFFTSSNIIKEVASLGGTVRGLVPDNVYQKL
                     LEKYPNKNNRR"
     gene            complement(1287..1862)
                     /gene="rsmD"
                     /locus_tag="PLR47_07445"
     CDS             complement(1287..1862)
                     /gene="rsmD"
                     /locus_tag="PLR47_07445"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOG12817.1"
                     /translation="MRPGSNVMRITGGKLKGRTLRFPSRSASRPTTDFLREALFNLLG
                     PQTDRSFLDLYAGSGAVGLEAASRGAKPVVFVEKSGKLIGVIRENVSSCGCSEQSRII
                     HDGVASALCALYDQPCRFDVVFADPPYGRGFIGETLRALQSCPVMEEGGTLVLQHSVR
                     EPLPALPGRWRVEKQRKYGENLLTFIRMESE"
     gene            complement(1898..2656)
                     /gene="pssA"
                     /locus_tag="PLR47_07450"
     CDS             complement(1898..2656)
                     /gene="pssA"
                     /locus_tag="PLR47_07450"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOG12818.1"
                     /translation="MNAERQIRSVRLKKGIYVLPNLITTASLFMGFYSIIATIQEKFF
                     VAAIAILVSMVFDGLDGRIARITNTTSKFGAEYDSLSDLIAFGLAPSLLAFNWAMSSY
                     GKLGWMAGFLFVACGALRLARYNIQIGLIDSKFFNGLPIPAAAAVIATTVIFVNDIMG
                     IEGIFHNPYVMICVVILSFLMVSNIKYYSGKDMKLFARMPFMTFLVVVGVLLVIIYAP
                     EVMIFVITVGYALSGPVWWLCKVISKKSGDEPAQ"
     gene            complement(2653..3294)
                     /locus_tag="PLR47_07455"
     CDS             complement(2653..3294)
                     /locus_tag="PLR47_07455"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOG12819.1"
                     /translation="MKHKSIIVAEGFPFIILSLVFTLFAAFFGVAWLTILLAALTIFI
                     IAFFRNPERRFQDAEKLVISPADGKVIKIEEVNAQGAMEGRFKKISIFMNVFNVHVNR
                     APCGGTIEKISYRPGKFVSANLDKASAENERNDVLIRTKDGSAVWTVQIAGLIARRIV
                     CWAREGDTVAKGERFGMIRFGSRVEVFLPAGSRVAVRLNDKVRAGQTPLGYLP"
     gene            3445..3699
                     /locus_tag="PLR47_07460"
     CDS             3445..3699
                     /locus_tag="PLR47_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOG12820.1"
                     /translation="MPGKVKIYTLSTCSHCKAAKKFLNENQIVFDATDVDLLQGADRE
                     AMLNEVIQYNPQRSFPTIIIGNQVIIGFKEAAIREALGIK"
     gene            3696..4028
                     /locus_tag="PLR47_07465"
     CDS             3696..4028
                     /locus_tag="PLR47_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HOG12821.1"
                     /translation="MKPAELYDLLKKAQEPKGYYFNKDKEWVLSILSDLLVNKERYGY
                     SSCPCRLATGDRGKDRDILCPCVYRAQDVKEYGSCYCNLYVSEDWNQGKIPHVYVPER
                     RPPEKISF"
     gene            4240..4389
                     /locus_tag="PLR47_07470"
                     /pseudo
     CDS             4240..4389
                     /locus_tag="PLR47_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            complement(4464..>4765)
                     /locus_tag="PLR47_07475"
     CDS             complement(4464..>4765)
                     /locus_tag="PLR47_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOG12822.1"
                     /translation="MLGEPIGAREAERIGLVNKVAPPEKFMQEAEKIAQTLAMRSAKA
                     IEELKNICAVVPRMDKIAALDMELSIGGMLFARDERKIHMNEELFLKKLRKKKQT"
BASE COUNT         1121 a         1345 c         1246 g         1053 t
ORIGIN      
        1 gccgatgatt tcttccggac ccgcggcata gccgatccgc cagccggtca tggcatacgc
       61 cttggaaacg ccgttcacca cgatggttct gtccttcagg cgtgaatccg ccatcacgat
      121 attggcaaag ggaaaatcgg cataaaggat cttttcgtag atgtcgtccg aaacggcaaa
      181 aatatccgta tccgccaaaa cggcggccag ggccttcagc tcgtccggcg aataggccgc
      241 gcccgtcgga ttggaggggc cgttgaagat gacggcccgg gtgcgggggg tgatcgaagc
      301 cttgagctgc tctggcttca ttttgaagcc gtctttctcc agcgtgttga gaattaccgg
      361 cgttcccccg gccagcacga cgatatccgg gtaggaaacc cagtagggcg ccggaatgat
      421 gacctcgtcc ccttcttcaa aaagcgcctg cgccagattg tacagcgtgt gcttggcgcc
      481 gcaggaaacg accacctgcg accgcttgta ttccagtcgg ttgtcggttt tcagcttggc
      541 gatgatcgag tccttgagtt catccgttcc gcccaccggc gtgtacttcg taaagcccgc
      601 ctcgatggcc cgaatggccg cctgcttgat gtgatcgggc gtatcgaaat caggttcgcc
      661 cgctccaaaa ccgatgacat ctcttccctg cgcccgaagg gcattggctt tggccgtaat
      721 ggccagggtt tccgagggtt tcacttttgc tactcttgac gccagtttca cgcactacct
      781 cctgttgttc ttattaggat atttttcgag cagtttctga tagacattgt ccggcaccag
      841 cccccggacc gtgcctccga ggctcgccac ttccttgatg atgttggagc tggtgaaaaa
      901 ccacctgaat ccgctcatga gaaacaccgt ctccacattc ttggtctggc ggcggttcat
      961 cagcgccatc tggaattcat attcaaagtc ggaaagggcg cgcatgcccc tcataattat
     1021 ggaggcgccg gaactctgca ggtaatcaat cagcaggccg tcatggctgt ccacctggac
     1081 gtattcgcag tcgctgaaga cctcgcggat gagccggcat cgctcttcca cggtgaaaag
     1141 aaaggatttg cgcggattat aggcaaccag aacgatgatt ttgtcaaaga tccggctgcc
     1201 ccttttgata atatccacat ggccgtttgt gatcgggtca aacgagccag ggtaaattgc
     1261 aactctgccg ccggtttttt ccgttatcat tcactttcca tccttataaa cgttaataga
     1321 ttttctccat attttctctg tttttccaca cgccaccttc cgggcagcgc gggcagcggc
     1381 tcccggacgg aatgctgaag gaccagggtc ccgccctctt ccatgaccgg acacgactgc
     1441 agggcccgaa gcgtctcccc gatgaagccc cggccgtagg gaggatcggc aaagacaacg
     1501 tcaaaccggc atggttgatc gtacagggcg caaagcgcgg aggcgactcc gtcatgaatg
     1561 atccgggatt gctccgaaca gccgcacgag gagacatttt cgcgaatgac gccgatcagc
     1621 tttccgcttt tttccacaaa aacaaccggt ttcgccccgc ggctcgccgc ctccagcccc
     1681 acggcacccg aaccggcata caagtccaga aagctccggt ccgtttgcgg ccccagcaaa
     1741 ttaaaaagag cttcccgcaa aaaatccgtc gtcggcctgg acgctgaccg cgaaggaaaa
     1801 cgcagcgttc ttcccttcag ctttcctccg gtgattctca taacgttgct gccgggccgc
     1861 atggggcttc ggatcgcttc tccttgccgc aaagagccta ttgcgccggc tcgtcgccgg
     1921 attttttgct gatgaccttg cacagccacc agaccggacc ggacagggca tacccgacgg
     1981 taataacgaa aatcatcact tccggcgcgt aaataatcac cagcaatacg ccgaccacca
     2041 ccagaaacgt catgaacggc atgcgggcaa aaagcttcat gtcttttccg ctgtagtatt
     2101 taatgttgct gaccatcaga aaagacagaa tgacgacgca gatcatcacg tacgggttgt
     2161 ggaaaattcc ttcaatgccc atgatatcat tgacaaaaat aacggttgtg gcaatgacgg
     2221 ccgcagccgc aggaatgggc agcccgttaa aaaacttgct gtcgatcagg ccgatctgaa
     2281 tattgtagcg cgccagtcga agcgctccgc aggccacaaa caggaaaccg gccatccagc
     2341 ccagttttcc gtaggaggac atcgcccagt tgaaggccag cagggacggc gcgaggccga
     2401 acgcgatcag atccgacagc gagtcgtatt cggcgccgaa tttgctggtg gtgttggtga
     2461 tgcgcgcgat gcggccatcc agtccgtcaa aaaccatgga aaccaaaatg gcaatcgcgg
     2521 ccacaaagaa cttttcctga atcgtcgcga tgatcgaata aaatcccata aataagctgg
     2581 cggtggtaat caggttcggc aggacataaa tgcccttctt aaggcgcacg ctgcgaattt
     2641 gtcgttctgc attcatggaa ggtatcctaa gggagtttgc cccgcccgga ctttgtcgtt
     2701 gagacgaacg gccactcggg aaccggcagg cagaaagact tcaacacggg aaccgaagcg
     2761 gatcatcccg aaccgttcgc ctttggcaac ggtgtcccct tctcgggccc agcagacaat
     2821 tcttctggcg atgagaccgg caatctgcac ggtccagacc gcgctgccgt ctttggttcg
     2881 gatcagaacg tcgttgcgtt cgttttccgc cgacgccttg tccagattgg ccgaaacaaa
     2941 cttccccggg cggtaggata ttttttcgat cgttcccccg cagggagcgc ggttgacgtg
     3001 aacattgaac acattcataa agatgctgat ttttttgaac cggccttcca tcgcgccctg
     3061 ggcattaacc tcttcaattt tgatgacttt gccgtcggcc gggctgatca ccagcttttc
     3121 cgcgtcctgg aagcgcctct ccgggttgcg gaaaaaagcg atgataaata tcgtcaatgc
     3181 ggcaagcaga atcgtcagcc aggccacgcc gaaaaaagcg gcaaatagag taaagacaag
     3241 ggagaggata atgaaaggaa aaccttcggc tacgataatg cttttatgtt tcataccgta
     3301 ttttttcttt cctcgattga gtttttgttg atatatgaat tgaggggcct tgtcaataaa
     3361 tctttgtccg ccgagaaccg aggatcatct tgacagaacc ccccgaaaac agttataaat
     3421 ccaaaaaatc acaggaggga aaaaatgccc gggaaagtga aaatctacac gctcagcacc
     3481 tgcagtcact gcaaggcggc caagaagttt ttaaacgaaa accaaatcgt ttttgacgcc
     3541 accgatgtcg atctccttca gggcgccgac cgggaggcca tgttgaacga ggtgatccag
     3601 tacaaccccc agcgatcctt cccgacaatc attatcggca accaggtgat catcgggttc
     3661 aaggaagctg ccattcggga ggctttggga attaaatgaa acccgcggaa ctgtatgatc
     3721 tcctgaaaaa agcccaggag cccaagggct attatttcaa taaagacaag gagtgggtgc
     3781 tttccattct ttcggacctg ctggtcaaca aggaacgcta cggctattcg tcctgcccct
     3841 gccgcctggc gacgggcgac cgcgggaagg accgcgacat cctttgcccc tgtgtttacc
     3901 gggcccagga cgtgaaagaa tacggaagct gctactgcaa cctttatgtt tccgaggact
     3961 ggaaccaggg caaaattcct cacgtgtacg tcccggaaag gcggccgccg gaaaaaatat
     4021 cattttgatt cacccgggat tgacaaacgg cccgcgcggc cataccttag agccgattta
     4081 tttcaggcgg agggaacatc ttttatgatc aatcagtaca tctgcaaaaa gaaaggcgct
     4141 ttcatcgcag aaatctgcac cgacacgacc tgtgaatggc ggctgaagaa cgaagcgttt
     4201 ttgaactgca cctgggtcgc ctgcaactac gggcctttta ccctcgagga agtcggcgac
     4261 atgatgggcg tcacccggga gcgcatccgc cagatcgaag ccaaggcgct caaaaagctc
     4321 cagcacaaga aacgccggga ccagctcaag gactttgccg cccccggcaa cgactgggac
     4381 aacctgtaaa ataaatttcg agaaaatcca ggggagaaga ttcgacgggc ggcccaacgc
     4441 ggcaagccct ttgccgcggc ggactaagtt tgttttttct tgcgcagctt cttcagaaac
     4501 agctcttcat tcatgtgaat tttccgttcg tcgcgggcaa acagcatgcc gccgatggac
     4561 aactccatgt ccagcgcggc aatcttgtcc attctgggca caacggcgca gatgttcttc
     4621 agctcttcga tggccttggc tgaccgcatg gcaagggtct gcgctatttt ttcggcttcc
     4681 tgcatgaatt tttcaggagg cgccaccttg ttgaccagac cgattctttc cgcttctctc
     4741 gcgccgatgg gctcccccag catcg
//
LOCUS       DAOSIM010000161         4751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_15970_length_4751_cov_7.048552, whole genome shotgun sequence.
ACCESSION   DAOSIM010000161 DAOSIM010000000
VERSION     DAOSIM010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15970_length_4751_cov_7.048552"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLR47_07480"
     CDS             <1..274
                     /locus_tag="PLR47_07480"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOG12823.1"
                     /translation="SFILFVMIVSILVFSLLGRPDFLHRVIYKLLLMPVIAGISYEAI
                     RFTGKYGRFKLVRLLSLPGLALQKITTREPDDDQIEVAIAAMKKVI"
     gene            434..2212
                     /locus_tag="PLR47_07485"
     CDS             434..2212
                     /locus_tag="PLR47_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOG12824.1"
                     /translation="MAHSFLWRGLLAILFAINFCLPALSAQEDSAQLTFRKTAVSKKS
                     VQYHTVRKDEAIYGIIRKMPGVTEKDIPHYLEIIKALNPRIKDWDKIYPGQKIALPSK
                     SIAGAGEEARGDATEDLSSAASGYPTYRVKKGDYLIRIVRRKLNIPTKTQQAMLRIKE
                     LNPSIKDANLLYVGQILRLPQARMQAGTGPAPSVRPGMDEAVPAPPPLSEGRPAQTGD
                     EQTAAAAMPEEQPAPAERTESAASLSPGARLEIIRHILTQMHGNLMTKGNYYLPISRT
                     AQLTIDCSAIPVVELDDQTTVFLEMKNRSSASLKKLIGGAWSNLHIVKMDAKDDVIVL
                     LKKIFAKSKDYEIIKAGKPIALGSEPDVEIPADWLINRKSKVRPSETVQGLRLVYQNR
                     DILPRAVTRLARAHSLAVTEFSPVSGLVAPREELYSLPPLTVLPKSPAADLGFAMLSY
                     LKLPADRNVEIRVFNIEKDGFNLSIKADILVSQGGRKTVVFSRTPPQEFIHVLRKTGH
                     ELIFISDRDDPATNLEKILRGVNVPFASGYFLFSGADHDQPPYTIGFKGIKIKNDEDV
                     YAVPFDVDHDLLGLMKEAWSATLIRY"
     gene            2290..3264
                     /locus_tag="PLR47_07490"
     CDS             2290..3264
                     /locus_tag="PLR47_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HOG12825.1"
                     /translation="MSIKAIALFSGGLDSILAVRLVQQQGIDVAGLTFTTPFFNAVKA
                     RAAADRIRLPLLVQDITGEHLQMLKSPRYGYGKNMNPCIDCHTLMLKIAGGKMEQTGA
                     DFLVTGEVLGQRPMSQTRQSLRMVAKNSGYADDILRPLSAQLLDPIQAELEGKIDRSR
                     LLAISGRGRKEQMRLAEAFGISHYPPPAGGCLLTDPMFARRLRDLMLHGEDREVRDYE
                     LLKYGRHFRVPEAGKIIVGRNQSDNEALRELAAENDLVCLMADFPGPLVLAPGGSEAS
                     QGAAASLCVRYSDAPSDKEARVTCRRGGQSAILTARAVSPDVSERWMI"
     gene            complement(3215..>4751)
                     /locus_tag="PLR47_07495"
     CDS             complement(3215..>4751)
                     /locus_tag="PLR47_07495"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOG12826.1"
                     /translation="IRSLGLRPSDVKKQLKGYRRGFSSLTLVRPCVPGDGIQSLSPAE
                     RRRLIRLYDAEASGCKMVKFVPASGAASRMFALWYAASERGTFGSDELDRSFFRDLRQ
                     MPFYPFLKQSDSTRRMIRRRDVRSVLQSILFPSGLDFGGLPKALIPFHAYRSGEVRTA
                     LEEHLREAADLITDGRGVCRLHFTVSAGHVKPVKTFLQSCVPRLEESRGVRFRVDLSV
                     QSPSTSIVAADGHHRPFRDEEGGLVFRPGGHGALLQNLQRIDADLIFVKNIDNIPPEF
                     LQKKILPCKKLLGGLMLETRRQVYSCLKRLERKDLSLTDLQAVSDFCRDALHSDLPKG
                     FQKLKAEEQRQWLFRLLNRPLRVCGMVRNEGEPGGGPFWVLEKDQSRTLQIVESAHVD
                     FNVPQQAGIWLEASYFNPVDMVCCIKNYRGEKFILDDYVNRDAYLISPKTEKGRELLA
                     QELPGLWNGGMAFWNTIFVELPLAAFNPVKTIYDLLRPQHRTGRAVKSSIVPTRPEKR
                     PAP"
BASE COUNT         1066 a         1371 c         1309 g         1005 t
ORIGIN      
        1 cagctttatt cttttcgtga tgattgtctc catcctggtt ttttccctgc tgggacgtcc
       61 ggacttcctg caccgggtca tttacaagct tctgctgatg ccggtaatcg cgggtatttc
      121 ttatgaagcc atacgcttta ccggaaaata cgggcgcttc aaactggtcc gtctgctgag
      181 cctgccgggg cttgccctgc agaaaatcac cacacgcgaa ccggatgatg atcagattga
      241 agtcgccatc gccgcgatga aaaaagtgat atgagatcgc tttcccgtcc gtgcgccgtc
      301 ttgtgatgtt ttgcgcaaac ctttacagag tgttcttcct gctgttttaa tcccgatgaa
      361 cattgacttt ttcataaagt tgaagtattt gaaaacgcaa gaatattctg gtttttttcg
      421 ggagcgaccc gcaatggcgc attcattttt atggagaggg ctgctggcaa tcctttttgc
      481 gataaatttc tgtcttccgg ctctgtccgc gcaggaagac agcgcgcagt tgacctttcg
      541 gaaaacggct gtctccaaaa aatccgttca gtatcacacg gtccggaagg atgaagcgat
      601 ttacggcatc atccgcaaga tgcccggcgt caccgaaaag gacattccgc attatctcga
      661 gatcatcaag gcgttgaatc ctcggatcaa agactgggac aaaatatacc ccggccaaaa
      721 gatcgcgctt ccttcgaaaa gcatcgccgg cgccggggaa gaagcccggg gggatgcaac
      781 ggaagatctt tcatcggcgg cgtccggata cccgacttat cgtgtcaaaa agggcgatta
      841 tctcatccgc attgttcggc ggaagttgaa cattccgacc aaaacgcagc aggccatgct
      901 tcgaatcaaa gaattaaatc catccatcaa ggacgccaat ctcctgtatg tgggccagat
      961 ccttcgactt ccccaggccc gaatgcaggc cggaacagga ccggcgcctt ccgtccggcc
     1021 cggcatggat gaggcggtgc cggccccgcc gcctttgtca gagggtcgac cggcgcaaac
     1081 gggtgatgag cagactgccg ccgcggcgat gccggaagaa cagcccgcgc cggccgaaag
     1141 aacagaatcg gccgcctctc tctcccccgg cgcaaggctg gagatcatca ggcacattct
     1201 gacgcaaatg catggaaacc tgatgaccaa aggcaattac tatctgccga tctccaggac
     1261 cgcccagctc acgattgact gttccgccat ccccgtagtg gagctcgatg atcagaccac
     1321 cgtctttctg gagatgaaaa accggtccag tgcttcttta aaaaaactga tcggcggcgc
     1381 gtggagcaac ctgcacatcg tcaaaatgga cgcgaaggac gacgtcatcg tccttttgaa
     1441 aaaaatattt gcaaaaagca aagactacga aatcatcaaa gccgggaaac ccatcgccct
     1501 gggatccgag ccggatgtgg aaattcccgc ggactggctg atcaaccgga aaagcaaggt
     1561 tcggccatca gagacggtcc aggggctgag actggtttac caaaaccgcg atattctgcc
     1621 gcgggccgtc acgcgccttg cccgggctca ttccctggcc gtcacggagt tttcccccgt
     1681 ctcgggtctt gtcgctccgc gggaggaact ttattccctc cctcccctga cggttttgcc
     1741 caaatcgccg gccgccgatc ttggcttcgc aatgctttcg tatctgaagc tgcccgccga
     1801 tagaaacgtg gaaatccggg tattcaatat tgaaaaggac ggcttcaacc tgtcgatcaa
     1861 ggcggacatc ctggtttcac agggcggcag gaaaaccgtg gttttttcca gaaccccgcc
     1921 gcaggaattc attcacgtcc ttcggaaaac gggccacgaa ctcattttca tttccgaccg
     1981 ggatgatccc gcgacgaacc tggaaaagat tcttcgaggc gtgaacgtcc ctttcgcgtc
     2041 cggctacttt ttattttccg gcgcggacca tgatcagccg ccctacacca tcggttttaa
     2101 aggcatcaag atcaagaacg acgaggacgt ttacgcggtt ccttttgacg tcgatcatga
     2161 tctgctggga ttgatgaagg aagcgtggtc ggcaacgctg atccggtatt agcgcgccgc
     2221 ttctgtcgat aaattgcacg atagcgctgc ggaaaatatc ccgccgcgct ttttcaataa
     2281 ggaaacttca tgagcatcaa ggccattgcc cttttttccg gcggactcga cagcattctg
     2341 gccgtccggc tggttcagca gcagggaatt gatgttgccg gtctgacctt cacgacgcct
     2401 ttcttcaacg ccgtcaaagc cagagcggcg gccgatcgaa tccgtctgcc cctgctggtg
     2461 caggacatta cgggcgaaca tctgcaaatg ctgaaatcgc cgcgctacgg ctacggcaaa
     2521 aacatgaatc cctgtatcga ctgccacacg ctgatgctca agatcgcggg cggaaagatg
     2581 gagcagacgg gcgcggattt tctcgtgacc ggcgaggtcc ttggacagcg ccccatgtcc
     2641 cagacgcggc aatcgctgcg tatggttgca aaaaattccg gctacgccga tgatatcctt
     2701 cggcctcttt ccgcccaatt gctggatccg atccaggcgg agctggaagg aaaaatcgac
     2761 cggtcgcgcc tgctggctat ttcaggacgc ggacggaaag agcagatgcg cctggcggaa
     2821 gccttcggga tttcgcatta cccgccgccg gccggaggct gcctgctgac ggacccgatg
     2881 tttgcaaggc gtctgcgcga tttgatgctg cacggagagg accgcgaagt ccgcgactat
     2941 gaactcttga aatacggacg tcacttccgc gttcccgaag ccggcaaaat catcgtcggc
     3001 aggaatcagt ccgacaatga agccctgcgg gagcttgcgg cggagaacga cctggtctgc
     3061 ctgatggccg acttccccgg tccgctcgtt ctggcccccg gcggcagtga agcgtctcag
     3121 ggcgcagccg cctcgctgtg cgtccgttat tccgacgccc cgtcagacaa ggaagcccgc
     3181 gtcacgtgcc ggcgcggcgg ccaatccgcc atcctcacgg cgcgggccgt ttctccggac
     3241 gtgtcggaac gatggatgat ttaacggccc tgccggtccg gtgctgcgga cgcagaagat
     3301 cgtaaatggt cttcacggga ttgaaagccg cgagcggaag ctcgacaaaa atggtattcc
     3361 aaaaggccat gcccccgttc cagaggccgg gcagctcctg cgcgagaagc tcgcgtccct
     3421 tctccgtctt ggggctgatc agataggcgt cccggttgac gtagtcatcc agaataaatt
     3481 tttccccgcg ataatttttg atacagcaga ccatatcgac cggattgaag taggaagcct
     3541 caagccagat gcccgcctgt tggggaacat tgaaatcgac atgggcggat tccacgatct
     3601 gcagggtccg ggattgatct ttttccagaa cccagaaggg cccgccgccc ggctctcctt
     3661 cgtttctgac catgccgcat actcgcaacg gccggttcag gagccgaaac agccactgtc
     3721 tttgttcttc cgccttcagc ttttgaaatc ctttgggcag gtcgctatgc agcgcatcgc
     3781 ggcagaaatc cgaaacggcc tggagatccg tcagggaaag atccttccgt tccagccgct
     3841 tcaggcagga ataaacctgc cgccgcgtct ccagcatcaa tcctcccagc aattttttgc
     3901 agggcagaat tttcttctgc aggaattccg gcggaatatt gtcgatgttt ttgacaaaaa
     3961 taagatcggc gtcgatccgc tgaagatttt gcaaaagggc gccgtgtccg ccgggacgga
     4021 agaccaggcc gccttcttca tcccggaaag gccggtgatg accgtcggcg gcaacaatgc
     4081 tggtcgaggg ggactgcacg gacaaatcca cgcgaaaacg aacgccgcgg ctttcttcaa
     4141 gccggggaac gcaggattgc agaaaagttt tcacgggttt gacatgcccg gcggaaaccg
     4201 tgaaatgaag ccggcagact ccgcgcccgt ccgtaatgag gtccgccgct tcccgcaggt
     4261 gttcctccag cgccgtccgg acttctccgc ttcggtaggc gtgaaaggga atgagcgctt
     4321 tgggaagccc gccgaaatcc aacccggatg ggaaaagaat gctttgcaga acgctcctga
     4381 cgtctctcct tctaatcatc cggcgcgtgg agtcgctttg tttcaagaag gggtaaaaag
     4441 gcatctgacg caaatcgcga aaaaaagacc ggtccagctc atcactgccg aaagtcccgc
     4501 gctccgatgc ggcataccac agagcaaaca tgcggctggc cgcgcccgac gctggaacaa
     4561 attttaccat tttgcagccg gaggcctcgg cgtcataaag ccgaatgagg cgcctgcgtt
     4621 ccgcggggct cagggactga atgccgtcac ccggaacaca ggggcgtaca agcgtcaaag
     4681 agctgaatcc gcgccggtag cctttcagtt gttttttcac atcggacggc cgaaggccaa
     4741 gcgatcggat a
//
LOCUS       DAOSIM010000162         4705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_16168_length_4705_cov_10.603011, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000162 DAOSIM010000000
VERSION     DAOSIM010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16168_length_4705_cov_10.603011"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..1543)
                     /locus_tag="PLR47_07500"
     CDS             complement(254..1543)
                     /locus_tag="PLR47_07500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG12827.1"
                     /translation="MSENYDLIVIGGGPGGLAAAQTASAANKRVLIIERGGWGGTCTH
                     RGCIPTKAMLACSRFYADLKKLKRVGVNVSGGAVDFAAMKKHRDQMVRIAALGAQKMI
                     AGPRVESKIGLGEILSPREVRCTGEDGVRQFLKTEKILIAWGSKPQILPGLRASERIR
                     TSDEILSLEQLPSSIIIVGGSFIGVEYATLFAELGVRVTLVELGVVLHTSTRLVRLEE
                     SGEGVKLSARKNGEDLEIQALYALLCTGRAPVLHADQLDSLGVDFTNAGIRVDKRMMT
                     SVGGIYAAGDVTGGMMLAHRASLQAKVAVEGMFGGSRYRYDEDLIPSVVYSHPQIARV
                     GLTERRALDEGLEISVVRSDYGANLMARAELMGQGFVKIIFHREVIAGAVAAGDHAAE
                     LLAPLALAVSGKWTRRQFRSWVLPHPTLGEVFAPLAD"
     gene            1725..3062
                     /gene="xseA"
                     /locus_tag="PLR47_07505"
     CDS             1725..3062
                     /gene="xseA"
                     /locus_tag="PLR47_07505"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOG12828.1"
                     /translation="MKDILTVSQLNDNIKTFLEESFGVLWVEGEVSNLRRPQSGHVYF
                     TLKDDKSQIRAVYFRPYGVRAVRFDLEDGMKILCRARLSAYPPRGEYQLIVESVEPQG
                     LGALQKAFEQLKARLAAEGLFDASRKKSLPFLPRRIGVITSPTGAVIRDILNITRRRF
                     PSVNILIAPARVQGAQATGEIITALRRLHAWGEVDVIVIARGGGSLEDLAPFNDEALA
                     REICRSTIPVVSAVGHETDFTVCDFAADLRAPTPSAAAELVVPRKIELQENLHDLRLR
                     LLAAQNRCLARQRERLASLAGRFRDPRRFLADYAIHVDDLRERLKRALAHQTESAAGR
                     LKPLQARLLGFDPRRRIREQSLLLSGLAKSLRGGWNHFLWERREKLQKNAALLSSLNP
                     LAVLRRGYSITRRLPEGEIVRDAKQLAPGNRLGIQLASGRIEARVEALQGEQA"
     gene            3118..3354
                     /locus_tag="PLR47_07510"
     CDS             3118..3354
                     /locus_tag="PLR47_07510"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOG12829.1"
                     /translation="MAKEKFEDALEKLEEIVREMEAGELPLDAALKSFEEGIRLIRFC
                     SAKLDETQRRVELLLEKDNSLQAKTFQDGQEDEA"
     gene            3344..4243
                     /locus_tag="PLR47_07515"
     CDS             3344..4243
                     /locus_tag="PLR47_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOG12830.1"
                     /translation="MKHEDFLEAYLKDRRNIVEEALDRYLPAGDQEPRELHAAMRYSV
                     FAGGKRIRPILCMASAEACGGDLAPVMPVACALELIHTYSLIHDDLPSMDNDDFRRGK
                     PTSHKVFGEAAAILAGDALLTEAFALLSRAEKVRLAAERRLAVIQEIALAAGAAGMVG
                     GQALDIRTDKEDPDIEALTDMHRRKTGALIMAAVKTGALIAGADERKLSALTGYANRI
                     GVAFQIADDILNVEGDERLMGKRTGSDAARGKITYASLLGLEAAKARLADEVGAAAAC
                     IASFDARALPLRAIARFIMERKS"
     gene            4262..>4705
                     /locus_tag="PLR47_07520"
     CDS             4262..>4705
                     /locus_tag="PLR47_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HOG12831.1"
                     /translation="MKTPEIISYSVLARVNDPADLRGLSVMELQKLAEEIRDLIISTV
                     ATTGGHLASSLGVVELTIALHYVFNTPQDRLIWDVGHQSYAHKIVTGRKEKFATLRQR
                     GGLSGFPKRSESPYDTFNVGHSGTSIAAAAAFAEAQCLKGEHNKII"
BASE COUNT          956 a         1496 c         1393 g          860 t
ORIGIN      
        1 cggtgaatat cctcccggcg cgatctcaaa gatccgcatg caaaaatggt ccgccccgtc
       61 attcttgccg ataacgacgc gggcgtcgat tcctttggcc tgatcgttgt caacgtgcac
      121 cggcgccgcc gatgcgtaat gaataatttt catcctgttc tccctgtcgt tcagtccgcc
      181 agcggcgcaa agacttcgcc gagcgtcgga tgcggaagca cccatgacct gaattgcctt
      241 ttctccctgt cgttcagtcc gccagcggcg caaagacttc gccgagcgtc ggatgcggaa
      301 gcacccatga cctgaattgc cttctcgtcc atttccccga tacggcaagc gcaagcgggg
      361 ccagaagctc ggccgcatgg tcgccggccg ccaccgcgcc cgcgatgacc tcccgatgga
      421 aaatgatttt gacaaacccc tgccccatca gctcggcgcg ggccatcaga ttggcgccgt
      481 agtcggaccg gaccacactg atctccaggc cttcgtccag ggcgcgccgc tcggtcagcc
      541 ccacgcgcgc aatctgcgga tggctgtaca ccacggatgg aatgaggtct tcatcgtacc
      601 ggtaacggct gccgccgaac atgccttcta cggcaacttt ggcctgcagg gacgcccggt
      661 gcgccagcat catgccgccg gtgacatcgc ccgccgcgta aattcccccg acactggtca
      721 tcattctttt gtccacacgg attcccgcgt tcgtaaagtc cacccccagc gagtcgagct
      781 gatcggcgtg caaaaccggc gcccggcccg tgcacagcag ggcgtacaag gcctgtattt
      841 ccaagtcttc gccgtttttc ctcgccgaca gtttgacgcc ttcccccgat tcttccaggc
      901 gaacgagccg ggtcgaggta tggagaacga cccccagctc caccagcgtc acccggacgc
      961 ccagttcggc aaacagcgtg gcgtactcaa cgccgataaa actgccgccg acaatgatta
     1021 tgctggacgg caattgttcg agactgagaa tttcatcgga cgtccgaatg cgctccgacg
     1081 ctcgaaggcc cggcagaatc tgcggcttcg atccccaggc gatcagaatc ttttccgttt
     1141 tcaaaaactg ccggacgccg tcttcgcccg tgcagcgcac ctcccggggc gaaagaattt
     1201 ctccaagtcc aattttgctt tccacccgcg ggcccgcaat cattttctgc gctcccaggg
     1261 cagcgattcg gaccatctgg tcgcggtgtt ttttcatggc ggcaaaatcg acggcgccgc
     1321 cggagacgtt cacgccgacg cgcttgagct ttttcaaatc cgcgtaaaac cggctgcagg
     1381 cgagcatcgc cttggtggga atgcagccgc ggtgcgtgca ggtccctccc cagccgcctc
     1441 tttcgatgat cagcacccgc ttgttcgcgg ccgacgccgt ctgagccgcc gcaagtcccc
     1501 ccggccctcc gccgatcaca atcaaatcat agttttcgga catgatgttc ctcccctcaa
     1561 aaaaatgttt aacacaggcc ttgaggccgg gcaaacggaa tttgcccgcc cgtatcaagg
     1621 gtgcaaaaag ggccgcggcg gaccgaaagc cgcgggcgtt cgggcggaat tgaaacttgt
     1681 catccccgtc attatctggt agttcacaaa gcaggatgga gcggatgaaa gacattctga
     1741 cggtttcaca actaaatgat aatattaaaa cctttctgga ggagtccttt ggcgtcctct
     1801 gggtggaggg ggaagtttcc aatctgcgcc gtccccagtc ggggcatgtt tatttcacgc
     1861 tcaaagacga caaaagccag attcgggcgg tgtatttccg cccctacggc gtccgggcgg
     1921 tgaggttcga cctggaagac ggcatgaaaa ttttgtgccg ggcgcgtctt tccgcctacc
     1981 cgccccgcgg cgaataccag ctcatcgtgg agtccgtgga gcctcagggc ctgggcgccc
     2041 tgcaaaaagc gtttgagcag ctcaaggcgc ggctggccgc cgaaggactg tttgatgcgt
     2101 cgcgcaaaaa gtctctgccc tttcttccgc ggcgcattgg cgtgatcacg tcgcccaccg
     2161 gcgcggtcat ccgcgacata ttaaacatca cgcgccgccg cttcccctcc gtgaacattc
     2221 tgatcgcgcc cgcccgcgtg cagggcgctc aggcgacggg cgaaatcatc acggccctgc
     2281 gccgtcttca cgcgtgggga gaagtggacg tgatcgtcat cgcgcggggc ggcggttctc
     2341 tggaggatct cgcccctttc aacgacgagg cgctggcgcg tgaaatttgc cgctcaacga
     2401 ttccggtcgt ctcggccgtc ggccatgaaa cggattttac cgtctgcgac ttcgcggcgg
     2461 acctgcgggc gcccactccg tcggcggccg cggagctggt cgtcccccga aaaatcgaac
     2521 tgcaggaaaa cctgcacgac ctgcgcctgc gtctcctggc tgcgcagaac cggtgtctgg
     2581 cccggcaaag agagcgcctt gcatcgcttg ccggccggtt cagggacccg cgccggttcc
     2641 tggccgatta cgccattcac gtagacgatc tgcgcgaacg gctgaagcgc gccctggcgc
     2701 atcaaacgga gagcgcagcc ggccggctga agccgctgca agcgcgcctg ctgggcttcg
     2761 acccccgccg gcggattcgt gaacaaagcc tgcttctttc cggacttgcc aaaagcctgc
     2821 gcggcggctg gaaccatttt ctttgggagc gccgcgaaaa gctgcagaaa aacgccgcgc
     2881 ttctttcctc cctgaacccg ctggccgttc tgcggcgcgg ctacagcatc acccggcgcc
     2941 tgccggaggg agaaatcgtc cgcgatgcaa agcagttggc gccgggaaac cggcttggca
     3001 ttcaactggc ctccggccgc atcgaggcaa gagtggaagc ccttcagggg gagcaagcat
     3061 agctcgcagc ggacatcgga gggtttctta aaaaaaatac aacgggagtg aaacacgatg
     3121 gcaaaagaaa agtttgaaga cgcgctggaa aaactggaag aaatcgtgag ggagatggaa
     3181 gcgggcgaac tgccgctgga tgccgcgctg aagtcctttg aagaaggcat ccgcctgatt
     3241 cgcttctgct cggcaaagct cgacgagacc cagcgccgcg tggagctgct gctggaaaaa
     3301 gacaactccc tgcaagccaa aacgtttcag gacggacaag aagatgaagc atgaggattt
     3361 tcttgaggcc tatctgaaag accggcgcaa tattgtggaa gaggcgctcg accgctatct
     3421 tccggccggg gaccaggagc cccgggaact gcatgccgcc atgcgctaca gcgttttcgc
     3481 cgggggcaaa aggatccggc cgattctctg catggcctcg gcggaagcgt gcggcgggga
     3541 ccttgcgccg gtgatgccgg tcgcctgcgc cctggaactg atccacacct attccctgat
     3601 ccacgacgac ctgccgtcga tggacaatga tgacttccgc cgcggcaagc ccaccagcca
     3661 caaagtattc ggcgaggcgg cggcgatcct cgcgggagac gcgcttttga cggaggcttt
     3721 cgcgctgctg tcgagggcgg aaaaagtccg tctggcggcg gagcggcggc tggccgtcat
     3781 tcaggaaatc gccctggctg cgggcgcagc gggcatggtg ggaggtcagg cgctggatat
     3841 tcgaacagac aaagaagatc cggacatcga ggccctgacg gacatgcaca ggcgcaaaac
     3901 cggcgcgctg atcatggcgg cggtcaaaac cggcgcgctc atcgcaggcg cagacgaacg
     3961 aaagctttcg gcgctgaccg gctacgcaaa ccgcatcggc gtagcttttc agattgccga
     4021 cgacattctc aacgtggaag gcgacgagcg cctcatggga aaaaggaccg gcagcgacgc
     4081 cgcccgggga aaaatcacct acgcatcact gctggggctt gaggcagcca aagcaaggct
     4141 ggccgacgag gtcggagcag ccgccgcctg catcgcctcc tttgatgcgc gcgctctgcc
     4201 gctgcgcgcg attgcccgct tcatcatgga acgaaaatcg taaaggttca ccatacccgc
     4261 aatgaaaact cccgaaatca tctcttacag cgtgctggcc cgagtcaacg acccggccga
     4321 tcttcgcgga ctttccgtca tggagctcca aaaactggcg gaggaaatcc gcgacctgat
     4381 catctccacg gtcgcgacca ccggcgggca cctggcctcg tcgctgggcg tcgtggaact
     4441 gacgattgcg ctgcactatg tcttcaatac gccgcaggac cggctgatct gggatgtagg
     4501 ccatcagtcc tacgcgcaca agatcgtcac cggccgcaag gaaaagtttg ccacgctgcg
     4561 ccaacgcggc ggcctcagcg gctttccgaa gcggtcggaa agcccctacg acacgttcaa
     4621 cgtcgggcac agcggaacct ccatcgcggc ggcggcggcc tttgccgaag cccagtgcct
     4681 gaagggcgaa cacaacaaaa tcatc
//
LOCUS       DAOSIM010000163         4700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_16197_length_4700_cov_7.997201, whole genome shotgun sequence.
ACCESSION   DAOSIM010000163 DAOSIM010000000
VERSION     DAOSIM010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16197_length_4700_cov_7.997201"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..374)
                     /locus_tag="PLR47_07525"
     CDS             complement(66..374)
                     /locus_tag="PLR47_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12832.1"
                     /translation="MDIFVRYCGGCNCQLDRSKLVSDVQRGLGPEFHLTTDLSGAPCP
                     AGLLVCGCPAACVWNSEVSAAARRWVIVAGKTVNAVEITEDRLAEAVAVEIKSILFSE
                     "
     gene            complement(394..741)
                     /locus_tag="PLR47_07530"
     CDS             complement(394..741)
                     /locus_tag="PLR47_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG12833.1"
                     /translation="MDVERCASVDNLQDLDQALLADDRVIAMVYATWCPFCRKVLPVF
                     EKWALKEKRRLLLVADDDERAADVYDIDIFPTLILFEKGRIVKRLDGRPGVGLSEKQI
                     ADFIKSCPPAEPG"
     gene            complement(788..>1007)
                     /locus_tag="PLR47_07535"
     CDS             complement(788..>1007)
                     /locus_tag="PLR47_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12834.1"
                     /translation="EGGGVAGVDGLMKPEQLANAVMESFAADEFLILPHKEVKTYIQR
                     KAADYNRWIQGMRRLQDRFLTGAPLKKV"
     assembly_gap    1008..1049
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1050..1550)
                     /locus_tag="PLR47_07540"
     CDS             complement(<1050..1550)
                     /locus_tag="PLR47_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG12835.1"
                     /translation="MEIRGKTIIVTGAASGIGRGLCRRFAKEGAKAIVAADINEAGAK
                     AVAGEAGGEAFACDVRKEADIIRLVKFTEDRWGGVDLFCSNAGIIAIGGYEVDNETWQ
                     RIWEINVLAHVFVARAVIPGMIRRGGGAIMITASAAGLLSQIGSLPYSVTKHAAVGMA
                     ENLAITY"
     gene            complement(1573..2388)
                     /gene="proC"
                     /locus_tag="PLR47_07545"
     CDS             complement(1573..2388)
                     /gene="proC"
                     /locus_tag="PLR47_07545"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOG12836.1"
                     /translation="MFTGKKVAILGGGKMGGILARGMITRKLLAPQNITVTDIDPQRL
                     EELRSALKLRVSDDNRQAVKGADIIVLAVKPQNFPDLLKEIRQAANSSKLFISIAAGV
                     TTDAIEKWLTKKPRVLRVMPNVAALAGEGAAAVAKGHFSRPDDVQYALAMLGAVGLAV
                     EVEEKLMDAVTGLSGSGPAYCFVMIEALADAGVQLGLGRDLAEKLAAQTMLGSARLCL
                     QGSHHPAQLKNMVTSPGGTTAAGLRVLEEGKIRATLTGAVEAAAKKAKELAGS"
     gene            2507..3298
                     /locus_tag="PLR47_07550"
     CDS             2507..3298
                     /locus_tag="PLR47_07550"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG12837.1"
                     /translation="MLKHFKVGDNNIACWINPADFGAHAQGLVFLHGSGGNSGAWSEQ
                     YSVLHKSFNIAAVDLPGHGQSEGSGEKDILAYVLRLKEILEVLKLKRPVLVGHSLGAA
                     IALGFASQHPQDASAVVAAGGGLTMPVNPDILDGFAKQPDLILDLMCKFSIAKENRPK
                     LFGPLRESLGQTSVDVVAGDMLACSKFDLTGELEKIRVPVLAVCGKEDKMTPPASSER
                     IAAGIAGAKLVLIEGAGHMAMLERPEAFNDALITFCREIVSDPRP"
     gene            3427..3723
                     /locus_tag="PLR47_07555"
     CDS             3427..3723
                     /locus_tag="PLR47_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOG12838.1"
                     /translation="MFALSNFIIALAKVIDIVLTVYLWIIVARALISWVNPDPYNKIV
                     IFLYRVTEPVLGPIRRMLPRHSLPIDFSPLIVLLIIIFLQYFLVQTLIQAARSF"
     gene            3737..4054
                     /locus_tag="PLR47_07560"
     CDS             3737..4054
                     /locus_tag="PLR47_07560"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOG12839.1"
                     /translation="MTDTLLIRETKNGLSFDIHVNPHASRAAIAGISGGMLKVKVTAP
                     PVEGAANDACIELLAKKLGLRKSLLKISAGAKGRKKTILVSDINRTELEQRFRSLDFP
                     PDA"
     gene            4087..>4700
                     /locus_tag="PLR47_07565"
     CDS             4087..>4700
                     /locus_tag="PLR47_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOG12840.1"
                     /translation="MDDPTEKSENAKVAKAAGIVGAATMVSRVLGVVRDMVIAAFFGA
                     SWMTDAFWVAFRIPNMLRRLLGEGSLTISFVPVFTEYLQKKTKPEALELADNAFTILS
                     VILAAVSALGIALSPVIVGLIAPGFMANPDQYQLAVFLNRLMFPYIFFIALVALCMGI
                     LNSFRHFTSPALSPVMLNIAMITAAVALHDKFDQPITALAAGVLA"
BASE COUNT          936 a         1422 c         1275 g         1025 t
ORIGIN      
        1 tgttttctat aacttacttc atctctttac gaaaacgttg ggacagtagt gatatctttt
       61 tatttttact cagaaaagag aatggatttg atttccacgg ccaccgcttc cgccagacgg
      121 tcctccgtta tttcaacggc attcaccgtt tttccggcaa cgattaccca gcgcctggcc
      181 gccgcgctga cttcggaatt ccagacgcag gcggccggac agccgcatac cagcagcccg
      241 gcggggcaag gcgcgccgga aagatccgtc gtcaggtgga actcagggcc aagcccacgc
      301 tgaacgtcgc tcaccagttt ggaccggtcc agttgacagt tgcagccgcc gcagtatcga
      361 acaaaaatat ccaaatgtgt cggactccga atttcagccg ggctcggccg gcggacagga
      421 tttgatgaaa tcggcgatct gtttctcact gaggccgacg cccggccttc cgtcgagccg
      481 cttgacgatg cggccctttt caaacaggat cagcgtcggg aaaatatcga tgtcgtacac
      541 gtccgcggcg cgctcgtcgt cgtcggccac cagaagcagg cgccgctttt cctttagcgc
      601 ccatttttca aacaccggca ggacctttcg gcagaaaggg caccaggtgg cgtacaccat
      661 ggcgatcact ctgtcgtccg ccaggagtgc ctgatccaga tcctgaaggt tgtccaccga
      721 cgcgcatctt tcgacgtcca cgggcttccc tccctctgct gatgcaaaaa tgccggaaac
      781 gcttttttta gactttcttt aacggcgcgc ccgtcaggaa cctgtcctgc agcctgcgca
      841 tgccctgaat ccaacggttg taatccgccg ccttgcgctg gatgtaggtt ttgacttcct
      901 tgtgcggcag aatcagaaat tcatcggcgg cgaacgattc catgacggcg tttgcgagct
      961 gctccggctt catcaggccg tccacccccg ccacgccgcc gccttcannn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng taggtgatcg ccagattttc cgccatgccc
     1081 accgccgcgt gcttggtgac ggaatacggc agagatccga tctgactcaa cagcccggcc
     1141 gcggaagcgg tgatcatgat cgcgccgccg cctcgccgga tcattccggg aatgaccgcc
     1201 cgcgccacga aaacatgcgc gagaacatta atctcccaga tgcgctgcca ggtttcgttg
     1261 tccacttcat agccgccgat cgcgatgatg ccggcgttgg agcagaaaag gtcaacgccg
     1321 ccccagcggt cttccgtgaa ttttaccaag cggatgatgt ccgcttcctt gcgtacgtcg
     1381 caggcaaaag cctctccgcc cgcttccccg gcgacggcct ttgcgcccgc ttcattgatg
     1441 tcggcggcga cgatggcctt cgcgccttct tttgcaaacc gccggcacag gccccggccg
     1501 atgccggaag ccgcgcccgt cacgatgatg gttttccccc tgatctccat ttttccccca
     1561 cggcttttta gttcatgaac cggcaagttc tttcgctttc ttcgcggccg cttccacggc
     1621 gcccgtcagc gtcgcgcgaa ttttcccctc ctccagcacc cgcaaaccgg cagcagtggt
     1681 tccgccggga gaggtcacca tatttttcaa ctgggcgggg tgatgacttc cctgaagaca
     1741 caggcgggcc gagcccagca tcgtctgcgc ggcaagcttt tcggccagat cacggcccag
     1801 ccccagttga acgccggcat ccgccagggc ttcgatcatt acaaaacaat aggccgggcc
     1861 gctgccgctg agtcccgtca cggcatccat gagcttctcc tccacttcca cggccaggcc
     1921 gacggcgccc agcatcgcca gggcgtactg tacgtcatcg gggcgggaaa aatgcccctt
     1981 cgccacggcc gcggcgcctt cgcccgccag cgcggcgacg ttgggcatca cgcgcaaaac
     2041 gcgcggtttt ttggtcaacc atttttcaat cgcatccgtc gtcacgccgg cggcgatgga
     2101 aataaacagc tttgaggaat tcgcagcctg ccgaatctct ttgaggaggt cgggaaaatt
     2161 ctgcggcttg acggccagaa caataatatc ggcccccttc acggcctgac ggttgtcatc
     2221 gctgacccgc agcttcagcg cagaacgcaa ctcttccagc ctctgtggat caatgtccgt
     2281 cacggtgata ttctgcgggg caaggagctt ccgggtaatc atcccccgcg ccagaatgcc
     2341 gcccattttg cctccgccga gaatcgcaac tttcttgcct gtaaacatgg ttatgtcctc
     2401 cggatggcca atacatgccc tttttcccgg catcctgtca atacaatatt gaagattgta
     2461 taaaatcccg cttttgtggt agtcacgggc tcgggaggga acactcatgc tgaaacattt
     2521 caaagtcggg gacaacaaca ttgcctgctg gatcaacccg gctgatttcg gcgcccacgc
     2581 gcagggcctg gtctttcttc acggctccgg cggcaactcc ggcgcctggt ccgaacagta
     2641 ttcggtcctg cacaaatcgt tcaacatcgc ggccgtcgac cttccggggc acgggcaatc
     2701 ggagggaagc ggagagaagg atattttggc ctatgtcctg cgtttaaagg aaatcctcga
     2761 agttttaaaa ttgaagcgcc cggttttggt cggacactcg ctgggtgcgg ctattgccct
     2821 gggttttgcc tcacaacacc cgcaggatgc aagcgccgtc gtggcggcgg gcggcggcct
     2881 gaccatgccg gtcaaccccg acattctgga cgggtttgcc aaacagccgg atttgatcct
     2941 ggatttgatg tgcaaattct ccattgccaa agaaaaccgc ccgaagctct ttgggccgct
     3001 gcgggaaagt ctgggccaga ccagtgttga tgtagtggcg ggcgacatgc tggcgtgcag
     3061 caagtttgat ctgactggag aactggaaaa gatccgcgtg ccggtactgg ccgtctgcgg
     3121 caaggaagat aaaatgacgc cgcccgcctc gtcggagcgg atcgccgccg gaattgccgg
     3181 agcgaaactt gttttgattg aaggcgcggg acacatggcg atgctggagc ggcccgaggc
     3241 gttcaatgac gcgctgatca ctttttgccg ggaaattgtt tcggatcccc gtccctgagc
     3301 ggcgtcgtcc ggatgccggc tgatggattc ttcacaggaa tcgccggcag aaaagctttc
     3361 acggaaggcc cgactctttg acacaaactc cctgcgggaa gcaaaagatc catcagtgag
     3421 gaagttatgt ttgcattgag caatttcatc atcgcgctgg caaaagtcat cgacattgtt
     3481 ctgacggtct atctgtggat catcgtcgcc cgcgctctca tttcctgggt gaatcccgat
     3541 ccttacaaca agatcgtcat tttcctgtac cgcgtcaccg aaccggtgct gggaccgatt
     3601 cgaaggatgc ttccccggca cagcctgccg attgattttt cgccgctgat cgttttgctg
     3661 atcatcatct ttctgcagta tttcctggtc cagacactga tccaggcggc gcgaagtttt
     3721 tagaaaggca aacgctttga ccgatacgct cctcattcgg gaaacaaaga acggcttatc
     3781 ctttgacatc cacgtcaatc cgcacgcctc gcgggctgcg atcgccggca tttccggcgg
     3841 catgctgaaa gttaaggtca ccgcgccgcc ggtggaaggc gcggccaacg acgcctgcat
     3901 cgaactgctg gccaaaaaac tggggctcag gaaaagcctg ttgaaaattt ccgcgggggc
     3961 caagggacgc aagaagacaa tcctggtcag cgacatcaat cgaacggaac tggagcaaag
     4021 attccgaagc ctggatttcc ctcctgacgc atagtcgccg gcaataagca aagcgcaatt
     4081 gaagaaatgg acgatcctac ggaaaaatca gaaaatgcaa aagtcgccaa ggccgccgga
     4141 atcgtgggcg cagcgacgat ggtcagccgc gttctcggcg tggtccgcga catggtcatc
     4201 gccgcctttt tcggtgcgag ctggatgacg gacgccttct gggtggcgtt tcgcattccc
     4261 aatatgctga ggcgcctgct gggcgaaggc tcgcttacga tttcatttgt tccggtgttt
     4321 accgaatacc tgcagaaaaa aacaaaaccg gaagcgctgg agctggccga caacgcgttt
     4381 acgatccttt ccgtcatcct ggccgccgtt tccgcgctgg gcattgcgct ctcgcccgtg
     4441 attgtcggac tgatcgcccc gggtttcatg gccaatcccg accaatacca gcttgcggtt
     4501 tttctgaacc gcctgatgtt cccctacatc tttttcattg cactggtcgc cctgtgcatg
     4561 gggattctga attctttccg gcatttcacc tctccggctc tgtctccggt gatgctcaac
     4621 atcgccatga tcacggccgc cgtcgctctg cacgacaaat ttgaccagcc gatcacggcg
     4681 ctggccgcgg gcgttctggc
//
LOCUS       DAOSIM010000164         4635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_16446_length_4635_cov_7.025983, whole genome shotgun sequence.
ACCESSION   DAOSIM010000164 DAOSIM010000000
VERSION     DAOSIM010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16446_length_4635_cov_7.025983"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PLR47_07570"
     CDS             <1..416
                     /locus_tag="PLR47_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12841.1"
                     /translation="GIANVADLPYPASAGFAAKYKETYKKAATWESALNYNTMHVLAK
                     AMTIAGSARDPYAIRKAVGKALPLLGDKYPSELFSIEENGVMHSAGIVQNVENGKFQK
                     AYYTLDFPKTRAEFDKYKKVSRIREPENYRWVPVE"
     gene            complement(446..2137)
                     /locus_tag="PLR47_07575"
     CDS             complement(446..2137)
                     /locus_tag="PLR47_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG12842.1"
                     /translation="MKKTDINSILKENESLKKRNRELENLLKKRVPGPVPVAQVQIYR
                     SLLHLCPASLVVSSLDDGVIYDVSDRFCRQSGFSRKEAIGRSSVELGLWVAPDEERER
                     FRDILLREGQCLNQEFRYRRKNGEVLTGMNFASLVTIDNRRYAIALVMDITARKQTEE
                     ALRESENRYRFLSEHMTDIAWIADVNLKTLYITPSLTKVLGFTPAERSRQRLDERMTP
                     DSVSRVREALARLLALEKKGKRIAEKPLTLLLEYYHRDGSTRWLETVVQGFHNTRGDL
                     TVIHGVSRDVTRRKQAQDELQRERIFLRSVIDGLPGVFYIADESDGLLFWNGNFEKTT
                     GFSEEEIRKMPVMGHIAEPDRRNLSNKIKQTALQGEISVEAGLVCKDGTVRDYLFQAN
                     RIEYQGRTCRMVAGTDITEHKQTQSALRSFAEELEDANKALRVLLRGRNEGQKAIEEK
                     LQANINDLVMPYLEKSGRAMREDPCKQYLNVLAGNLKEIVSPFMKNVQSLQKYLTPQE
                     IQVADLIRKGMRTKEIAGVLNTSDSTIGTHRNNIRKKLNLTRQGVNLRSYLQSLQ"
     gene            complement(2155..2943)
                     /locus_tag="PLR47_07580"
     CDS             complement(2155..2943)
                     /locus_tag="PLR47_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOG12843.1"
                     /translation="MEGLTVERKGNVEVITLNRPEVMNALTYDMVQGLGDLFEAAQAD
                     DDIRALLLTGAGRAFSTGADLTGKGSARKDAHTPLGMRISTFGYSRMVNSIWALEKPV
                     VCAVNGTAAGASCNLALSCDLVLAADTAKFIQVFVRRGLIPDGGGTYFLPRLVGLPRA
                     KQLMLLGEDLPASRALEWGLIYNVVPADKLMDEAMEMAKRLAAGPTRAIGMIKSMLNR
                     SLESDLAAALEREASLQGIAAGTGDFIEGVMSFLEKRPPAFKGR"
     gene            3178..4557
                     /locus_tag="PLR47_07585"
     CDS             3178..4557
                     /locus_tag="PLR47_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOG12844.1"
                     /translation="MEPSAMNALLQGQKAFFRSGKTRSISFRIASLKKLKAVISDHEK
                     EILAAVYDDLHKEATDAFATEISGVYTEIKLAIGNVSDWAARQKVPTPFFMRPSKSYI
                     YKEPYGVALIIAPWNYPFQLLILPLVGAIAAGNTAILKPSEISSHTAAILEKIIRAAF
                     PPEYICVVQGGAPETKALLELPVDYIFFTGSVPVGKAVMAAAARNLTPLTLELGGKSP
                     AIVHQTANLQTAAGRISWGKFLNAGQTCIAPDYVLAHESIRDAFLEELAKVIVKFYGR
                     DASTHPRYCRIINDRHFERLTALLDKTKVVCGGKTDAASRYIEPTVLSPADWDDPVMQ
                     EEIFGPLLPVLSYYDLDDAIRRINERPKPLALYLFANDKAVRRKIIRETSFGGGAINN
                     TIMHIVSHHLPFGGVGPSGLGRYHGQYSFETFSHAKSILRSYNRTDVADIARPYRGRM
                     MRLILKWMK"
BASE COUNT          972 a         1386 c         1276 g         1001 t
ORIGIN      
        1 tcggcatcgc caatgtcgcg gatcttccct accccgcttc cgcgggtttt gccgcaaaat
       61 acaaagaaac ctacaaaaaa gcggcgacgt gggaatccgc gctgaactac aacacgatgc
      121 atgtcctggc caaagccatg accatcgccg gctcggcccg tgatccttac gccattcgaa
      181 aggccgtggg caaggcgctg cctcttctgg gcgacaagta ccccagcgaa ctgttcagca
      241 tcgaggaaaa cggcgtcatg cattccgccg gcatcgtcca gaatgtggaa aacggaaaat
      301 ttcaaaaggc ctattacacg ctggactttc caaagaccag ggcggaattc gacaagtaca
      361 aaaaggtgag caggatcagg gaaccggaaa actaccggtg ggttcccgtc gagtaacccc
      421 gcagccggcc gttttgccgg gagcgctact gaagcgactg caggtaggaa cgaagattga
      481 cgccctggcg ggtcaggttc agttttttcc ggatattgtt ccggtgcgtt ccgatcgtgc
      541 tgtccgacgt gttgagcaca ccggcgattt ccttggtcct catgcccttg cgaatcagat
      601 cggcgacctg aatttcctgg ggggtcaggt acttctgcaa agactgcaca tttttcatga
      661 agggagacac aatctctttc agattgccgg ccagaacatt caggtattgt ttgcacggat
      721 cttcccgcat cgcccttccg ctcttttcta aatacggcat gaccaaatcg ttgatgttgg
      781 cctgcagctt ctcttcaatt gccttttgcc cttcgttccg gccgcgcagc aatacgcgca
      841 gcgccttgtt ggcgtcttcc agttcttcgg cgaagctcct gagggcgctc tgggtctgct
      901 tatgttcggt aatgtcggtg ccggcgacca tccggcaggt ccgcccctga tattcgatcc
      961 ggtttgcctg aaacaaataa tccctgacgg ttccgtcctt gcagacgaga ccggcttcca
     1021 cggaaatttc tccctgcagg gctgtttgct ttattttatt cgacagattt cgccggtccg
     1081 gttcggcaat gtggcccatc accggcatct tcctgatttc ctcttccgaa aaaccggttg
     1141 ttttctcgaa gttgccgttc cagaacaaaa gtccatccga ctcatccgcg atgtaaaata
     1201 cgcccggcag gccgtcgatc accgaccgaa ggaaaatccg ctcccgctgc aattcgtcct
     1261 gcgcctgctt gcgcctggtc acatcccggg acacgccgtg aatcaccgtc aaatcgccgc
     1321 gggtattgtg aaagccctgt acgaccgttt ccagccagcg ggtcgagccg tccctgtgat
     1381 aatattccag cagaagggtc agcggctttt cggcaattcg ctttcccttt ttttcaagag
     1441 cgagcagtct ggccagagcc tcccgcacgc gggatacgga atccggcgtc attctctcgt
     1501 ccaggcgctg acggcttctc tccgcgggag taaaacccag cactttagtg agcgacgggg
     1561 taatgtagag ggttttcagg ttcacatccg caatccacgc gatatcggtc atgtgctccg
     1621 aaaggaaccg gtaccggttc tcgctttccc gcagggcttc ctcggtttgt ttccgggccg
     1681 tgatgtccat taccagggcg atggcgtagc gccggttgtc tatcgtaacc agcgacgcga
     1741 aattcatgcc ggtgagcacc tctccgtttt ttcttcgata ccggaactcc tgattcaggc
     1801 actggccctc gcgcaggaga atgtcccgaa atctttcccg ctcctcatcg ggcgccaccc
     1861 acaatcccag ctccacggat gaccggccga tggcttcttt gcgtgaaaaa ccggactgcc
     1921 ggcaaaaccg gtcgctgaca tcatagatga cgccgtcgtc caggctgctc acgaccaggg
     1981 aggcggggca caggtgaagc agggatcggt agatttgtac ctgcgcaaca ggaaccgggc
     2041 ccggcaccct cttctttaga agattctcca gctcccgatt tctttttttg agggattcgt
     2101 tttccttcag gatcgaattg atgtccgttt ttttcatatg gcaaccgccg gaagtcagcg
     2161 gcccttgaat gcgggcggac gcttctccag aaaagacatg actccctcga tgaagtcgcc
     2221 ggttccggcg gcgatcccct gcagcgaggc ctcgcgctcc agcgccgccg cgaggtcgga
     2281 ttccagcgaa cggttcagca tgcttttgat catgccgatg gcccgggtgg gacccgcggc
     2341 aagccgcttc gccatctcca tggcctcatc catcagtttg tccgcaggca ccacgttata
     2401 gatcagcccc cattccagcg ccctggaagc gggcaaatcc tcgcccagca gcatgagctg
     2461 cttcgctcgg ggaagaccca ccagccgggg caaaaagtag gtgccgcctc catcggggat
     2521 cagcccccgc cgcacaaaca cctgaatgaa ctttgccgtg tcggcggcca gcaccaaatc
     2581 gcaggacagg gccagattgc agctggcgcc cgccgcagtg ccgttgacgg cgcataccac
     2641 cggcttttcc aacgcccaga tggagttcac catccgcgag tagccgaacg tggagatgcg
     2701 catgcccaga ggcgtatggg cgtcttttct ggcgctgcct ttgccggtga ggtcggcgcc
     2761 ggtggaaaac gcccgtccgg caccggtcag gagaagcgcg cggatatcgt cgtcggcctg
     2821 cgccgcctca aagagatctc ccagcccctg caccatgtcg tacgtgagcg cgttcatgac
     2881 ctccgggcgg ttcagtgtga tgacctcaac atttcccttg cgttcgacgg tcagtccctc
     2941 catggttcac tttcctccgt tggcttttat ggaaaagcgt tttgctgttt tctcatgcgg
     3001 actgtaggca aaacaccgcc ggatgtcaat gttaaactgg cgatgaagaa gcaacgagcg
     3061 atgagtgagg aatagggggc tttcgcttga cacgccggcg ccatgcgctt atagttgacg
     3121 cacatggacg gcgggctgtt tttagaatct tccgcattac cttctggagg cgcatgaatg
     3181 gaaccatccg cgatgaacgc gctgctgcag ggacaaaagg cttttttccg atcgggaaaa
     3241 acgagaagca tttcttttcg cattgcttcg ctgaaaaagc tcaaggccgt catcagcgat
     3301 catgaaaagg aaattctggc cgccgtgtat gacgatctgc acaaggaggc caccgacgcc
     3361 ttcgcgacgg aaatttccgg cgtgtacacc gaaatcaagc tggcgattgg aaacgtgagc
     3421 gactgggcgg cgcggcaaaa agttccgacc ccgtttttta tgagaccgtc caaaagctat
     3481 atctacaaag agccctacgg cgtggcgctg atcatcgcgc cctggaatta tccttttcag
     3541 cttttgatcc tgccgctggt cggcgcgatc gccgcgggca atacggccat cctgaaacct
     3601 tcggaaattt cgtcgcacac ggcggccatc ctggaaaaaa tcatccgcgc cgcgtttccc
     3661 ccggaataca tttgcgtcgt tcagggggga gcgcccgaaa cgaaggcgct tctggaattg
     3721 cccgtcgatt atatcttttt cacgggcagc gttcccgtcg ggaaggcggt catggcggcg
     3781 gcggccagaa acctgacccc gctgacgctg gagctgggcg gcaaaagccc cgccattgtt
     3841 catcagacgg ccaaccttca aacggcggcc ggccggatca gttggggaaa atttctgaat
     3901 gcggggcaga cctgcatcgc gccggattat gtgctggcgc acgaatccat tcgggacgcc
     3961 tttttggagg aactggcgaa agtgatcgtg aaattttacg gccgggacgc ctcaacgcat
     4021 ccgcgctact gccggatcat caacgaccgg cactttgaac gactgacggc gcttctggac
     4081 aaaaccaagg tggtgtgcgg cggcaaaacc gacgccgcct cgcgctacat cgagcccacc
     4141 gtgctgagtc cggcggactg ggatgacccc gtcatgcagg aggaaatctt cggcccgctg
     4201 cttccggtcc tttcctatta cgatctggac gacgcgatcc gccgaatcaa tgaaaggccc
     4261 aagccgcttg ccctgtatct gtttgccaac gacaaggccg tccggagaaa gatcattcgg
     4321 gaaacctcgt tcggcggcgg cgccatcaac aacaccatca tgcacatcgt gtcccatcac
     4381 ctgccgttcg gcggcgtggg tcccagcggt ctgggccggt atcacggcca atacagcttt
     4441 gaaacgtttt cccacgccaa aagcatcctg cgcagctaca accggacgga tgtcgcggat
     4501 atcgccaggc cttaccgggg aagaatgatg cggctgattc tgaaatggat gaaatgatgc
     4561 gcaaagaaaa atctccggaa tcctaacgat atggccgtca tcgaaacccg atccctgaca
     4621 aaaaccttcg gcaaa
//
LOCUS       DAOSIM010000165         4632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_16461_length_4632_cov_6.018134, whole genome shotgun sequence.
ACCESSION   DAOSIM010000165 DAOSIM010000000
VERSION     DAOSIM010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16461_length_4632_cov_6.018134"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PLR47_07590"
     CDS             <1..425
                     /locus_tag="PLR47_07590"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOG12845.1"
                     /translation="SRLPGSGGANPIGALAKRVLIIALHNTRSLAEKVDFITTPGYLD
                     GPGARERWGLPPETGPQVIITNKAVLRFDKETKEAYLESYHPGTSVDEVVKLTPWELK
                     VSDDVHETEPPRAEEIRVIREVIDPHRMITIYEKRGYV"
     gene            487..>1310
                     /locus_tag="PLR47_07595"
     CDS             487..>1310
                     /locus_tag="PLR47_07595"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HOG12846.1"
                     /translation="MEYTQQLIRFCSDLSFDTLPEEVRHKAKLCILDYVANIYGSLEL
                     DAVRQVVDFVRSLGGPEAATALACGFKTGLQNAAFINGLTGEAIEAQDGLRFGGNHSG
                     VSVIPAALAVAENLGLSGKRLIEAVAAGYEASNRTAAAMHPWHTLSGFLPTGTCGTLG
                     AAVAVSKLMGHAYPEMLNAIGIAGYILPVSMAEQLMGGYTVKIVQGGQAASAAILAAG
                     LAGKGITGSPFVLEGTELKGGITQITMKVDAKPERITEKLGEHYSINDVYFKPYTA"
     assembly_gap    1311..1320
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1321..1771
                     /locus_tag="PLR47_07600"
     CDS             <1321..1771
                     /locus_tag="PLR47_07600"
                     /inference="COORDINATES: protein motif:HMM:NF040249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12847.1"
                     /translation="YGVAALAVGKGVPEGGSFVSAQFSIPHVVAACILDGALGPAQLT
                     ERRIVDPAIIALRKKVTVKNDDELNKMYPDKTASRVEILLKNGERLVKTVDIPKGDPR
                     DPMDASDIAQKVKFFAGGRDQNKVNQIIDAILNLEKANNINELVSLI"
     gene            1798..2958
                     /locus_tag="PLR47_07605"
     CDS             1798..2958
                     /locus_tag="PLR47_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG12848.1"
                     /translation="MDFTLNEEQKMLVDTIRTMGQREKFKELARHIDETGEFPYDLLK
                     KYADLGLLGMTISAEHGGGGQPALNAILCIEELAKFSPMIAAPVFESNVGPVRVIDIF
                     GTVEQKKTIVPGVCRGELSVSVCMTEPEAGSDLTALTTRADEDGDDYILNGRKCFITG
                     GGHASHYMVYTRFGGMKGYKGIGGLLVENGSPGFTFGKQEKFMGLRGMPSCDLIFDNV
                     RVPKKNLVIAPGEFGKLMWTFDIERCGNSAMCLGIAGGAFEEAKAYAMERKAFNRPIC
                     EFQAVQFMMADMAAKIDAARLLVYRAVSGAGQGLPSIYEASMAKSYANEMVVDVTSTA
                     MQVFGGYGYSTEFPVERMYRDAKAWGVAGGTIQMLKIGIAGMIFGRRFDQRK"
     gene            2999..3169
                     /locus_tag="PLR47_07610"
     CDS             2999..3169
                     /locus_tag="PLR47_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12849.1"
                     /translation="MKKKIIMILCTVVVLIGALLVKTLWNAGEFQSLSRLYAAARQEH
                     REWAALMLPVWF"
     gene            3238..3627
                     /locus_tag="PLR47_07615"
     CDS             3238..3627
                     /locus_tag="PLR47_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12850.1"
                     /translation="MSQYYKDADDLYRIYGYFLGRVLQDEKIGTKMSKAGIIIKFMYT
                     DPDAEITIDLKNPPSKPGYYGSFYLGPCDLKEDVWSKQPADHSHIKQGGKVTAMLKLL
                     PVIKPAFQLFPVALKEMGLDHLIVTKK"
     gene            3718..>4632
                     /locus_tag="PLR47_07620"
     CDS             3718..>4632
                     /locus_tag="PLR47_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOG12851.1"
                     /translation="MKGNTGRIADIDLSTGNVSILQLPEETYRRYIGGSGLAAKIFWD
                     RADFSADPLSPEAILILMNGPLTGVKLSGVSRMNATARSPLTGGIAESSCGGYFPPAL
                     RLAGFDGLILSGRAARPSVLLIENESVSVRDAGNLWGRGVLECTRELHKTYGKKSTSL
                     IIGPAGENGVPFACILNEAHHAFGRCGMGAVMGSKNLKALIVQSGGGTLSLADPDRIK
                     SLARELNPRIKDYLISQVLHDYGTSGNLEGHMYDGDVPMRNWTSNYDEATASALTGST
                     LSETYLKKTATCAYCAVACKRIVQVDEGP"
BASE COUNT         1061 a         1248 c         1394 g          919 t
ORIGIN      
        1 aaagccgcct tcccggctcc ggcggcgcga accccatcgg agcgctggcc aaaagggtgc
       61 tcatcatcgc gcttcacaac acgcgcagcc ttgccgaaaa ggtggacttc atcacaacgc
      121 ccggttatct ggacggcccg ggcgcccgcg agcggtgggg gcttccgccg gaaacgggtc
      181 cgcaggtcat catcaccaac aaggccgtcc tgcggttcga taaggagaca aaagaggcat
      241 acctcgaaag ttatcatccc gggacgtctg tggacgaagt ggtcaagctg accccgtggg
      301 agctcaaggt aagcgatgac gtgcatgaaa ccgagccgcc ccgtgccgag gaaatccgcg
      361 tcattcggga agtcatcgat ccgcaccgga tgatcacgat ctacgagaag cgcggatatg
      421 tttaggagct ttcaccggga ggcttccgcc cgtgaacatt ccgttttctg aaggaggaga
      481 caatctatgg agtacacgca acaactgata cggttttgtt ctgatctgtc ttttgatacc
      541 ctgccggaag aagtgcggca caaggcaaaa ctctgcattc tggactacgt ggccaatatt
      601 tacggatcgc tggagctcga tgcggtgcga caggtcgttg atttcgttcg ttcactcggc
      661 ggcccggaag ccgcgacggc tctggcctgc ggttttaaaa ccggcttgca gaatgccgct
      721 ttcatcaacg gactgaccgg tgaagctatc gaggcgcagg acgggctccg cttcggaggc
      781 aatcattcgg gagtgtccgt cataccggcg gcgcttgccg tggctgaaaa cctggggctt
      841 tccggcaagc gtctgatcga ggcggtggcg gcgggttacg aggcgtccaa ccgaacggcg
      901 gcggccatgc atccatggca cacgctgtcg ggatttctgc ccaccgggac ctgtggaacc
      961 ctcggcgcgg ccgtggcggt ctccaaactg atgggccacg cctatccgga gatgctcaac
     1021 gccatcggca ttgccggcta tatcctgccg gtttccatgg cggagcagct catgggcggc
     1081 tataccgtga agattgtcca gggcggccag gcggccagcg ccgccatctt ggcggcgggg
     1141 cttgcgggaa agggaattac cggcagtcct ttcgttctgg aagggacgga gctcaagggg
     1201 ggcatcacgc agatcaccat gaaagtggac gccaaaccgg agcgcatcac ggagaagctg
     1261 ggagagcatt attccatcaa cgacgtttat ttcaagcctt acaccgcctg nnnnnnnnnn
     1321 ttacggcgtg gccgctctgg ccgtggggaa gggcgttccg gaaggcggca gtttcgtctc
     1381 tgcgcagttt tccattccgc acgtcgtggc ggcctgcatc cttgacggcg cgctgggtcc
     1441 cgctcagctt acggagcggc gcattgtcga tccggcgatc atcgcgctga gaaaaaaagt
     1501 aacggtgaag aatgatgacg aactgaataa aatgtacccg gacaaaaccg cgagccgcgt
     1561 ggaaattctg ctaaaaaacg gcgaacgctt ggtgaaaacc gtggacattc ccaaaggcga
     1621 tccgcgcgat ccgatggacg catcggatat cgcgcaaaaa gtgaagtttt tcgcgggagg
     1681 gcgggatcaa aacaaggtga atcaaatcat cgatgcgatt ttgaatctgg aaaaggccaa
     1741 taatataaat gaattagttt ctttgatcta aaccaatact tgaacaaaag gactcacatg
     1801 gattttacat taaatgaaga acagaagatg ctggtcgaca ccatcaggac catgggccag
     1861 cgtgaaaaat tcaaggagct ggcccggcac attgatgaaa cgggggaatt cccgtatgac
     1921 ctgctgaaaa aatacgccga tctgggactc ttgggaatga cgatttcggc cgaacacggc
     1981 ggagggggcc agcctgctct caacgcgatt ttgtgcatcg aggagctggc caaattcagc
     2041 cccatgattg cggcgccggt gtttgaatcc aatgtcggac cggttcgggt tatcgatatc
     2101 ttcgggaccg tggagcagaa gaaaaccatt gtgcccggcg tctgccgggg cgagttgagc
     2161 gtgtccgtat gcatgaccga gccggaagcc ggctccgacc tcaccgccct cacgacgcgc
     2221 gccgacgagg atggcgacga ttacatcctc aacggccgca aatgcttcat taccggcgga
     2281 ggacacgcca gccattacat ggtctatacg cgcttcggcg gcatgaaagg ctacaaaggc
     2341 atcggcggcc tgctggtgga gaatggatca cccggcttca cgttcggcaa acaggaaaaa
     2401 ttcatgggtc ttcggggcat gccctcctgc gatctcatct ttgacaacgt gcgtgttcca
     2461 aagaaaaatc tggtgatcgc gccgggcgag ttcggcaagc tgatgtggac ctttgacatc
     2521 gagcggtgcg gaaattctgc gatgtgtctg ggcattgcgg gcggcgcttt cgaggaggcg
     2581 aaagcctacg ccatggaaag aaaggcgttc aaccggccga tctgcgaatt ccaggcggtg
     2641 cagttcatga tggcggatat ggccgcaaaa atcgacgcgg ccaggcttct ggtctatcgg
     2701 gccgtgagcg gcgcgggaca ggggcttccg tcgatctatg aagcctcgat ggccaagtct
     2761 tacgccaacg agatggtggt ggacgtgacg agcacggcca tgcaggtgtt cggggggtac
     2821 ggctacagca cggaatttcc cgtggagcgc atgtaccgcg acgcgaaggc ctggggggtg
     2881 gcgggcggca cgattcagat gctgaaaatc ggaatcgccg gcatgatctt cggcaggcgc
     2941 ttcgaccagc gcaaataatc cggcagtttc cggaggcgtg aacaagaagg aacaagtcat
     3001 gaaaaagaag atcatcatga tcctttgtac ggttgttgtt ctgattgggg cgttgctcgt
     3061 aaagacgctg tggaatgccg gggaatttca atcactttct cgattgtacg ctgctgcccg
     3121 gcaagagcat agagaatggg cggcgttgat gttgcccgtt tggttttaat gactgatccg
     3181 gggaagttgc ggcgcggatc tctcttcgac ctttaacgaa tcaaaaagga ggcacaaatg
     3241 agccagtatt acaaggatgc ggatgatctc tacaggattt acggatattt tctgggccgg
     3301 gttcttcagg atgagaaaat cggaacaaaa atgtcaaagg cgggcatcat catcaaattc
     3361 atgtacacgg acccggacgc ggaaataacg atcgacctga aaaatccgcc gtcaaagccc
     3421 ggctactatg gctcctttta tctggggccc tgcgatctca aggaggatgt gtggtccaag
     3481 cagccggccg accattccca catcaagcag ggcggaaagg tcaccgccat gctgaagctg
     3541 ctgccggtca tcaagccagc atttcagctc tttcccgtag cgctcaagga aatgggtctt
     3601 gatcacctga tcgttacgaa aaaataaaat ttcttccggt ggtcataagg ccacgggcgc
     3661 acgatttacg gtttgattgc aagacaggaa ttacgacaaa cggcaaggag taacgatatg
     3721 aagggaaaca caggccggat tgccgacatt gacctttcca ccgggaacgt cagcattctt
     3781 caactgccgg aagaaaccta ccgccgctat atcggcggaa gcggcctggc cgccaaaatc
     3841 ttctgggacc gggccgattt ttcggccgat cccctttccc ccgaagcgat cctgattctg
     3901 atgaacggcc ccctgaccgg cgtcaaactc tccggcgtca gccgaatgaa cgcgacggcg
     3961 cgttctcccc tgaccggcgg aatcgcggaa tcctcctgcg ggggctattt cccccccgcg
     4021 ctcaggctcg ccggatttga cggcctgatt ttatccggcc gggcggcgag gccttccgtg
     4081 ctcttgatcg aaaacgaatc cgtgtccgta cgggacgccg ggaacctctg gggcagaggc
     4141 gttctggagt gcacgaggga gcttcataaa acctacggga aaaaatcgac gtcgctgatc
     4201 atcggtccgg ccggagaaaa cggggttccc tttgcctgca ttctcaacga agcccatcat
     4261 gcctttggcc gctgcggcat gggcgccgtc atgggctcca agaacctgaa ggcgcttatc
     4321 gtgcagtccg gcggcgggac gctttccctg gctgatcccg accggatcaa atcgctcgcc
     4381 cgggagttga atccgcgcat caaagattat ctgatttccc aggttcttca cgactacgga
     4441 acatccggga atctggaagg gcacatgtac gacggagatg tgccgatgcg caactggacg
     4501 tccaactatg acgaggcaac ggccagcgcc ctaacgggga gtaccctgtc agaaacctat
     4561 ctcaaaaaaa ccgccacttg cgcgtactgc gcggtcgcct gcaagagaat cgtgcaggtg
     4621 gacgaagggc cg
//
LOCUS       DAOSIM010000166         4607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_16564_length_4607_cov_7.465070, whole genome shotgun sequence.
ACCESSION   DAOSIM010000166 DAOSIM010000000
VERSION     DAOSIM010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16564_length_4607_cov_7.465070"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PLR47_07625"
     CDS             complement(<1..294)
                     /locus_tag="PLR47_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOG12852.1"
                     /translation="MDIKKICVLGAGLMGNGIAQVCAAAGYEVTLRDIEQRFIDNGMN
                     NIKKNLGRDAEKGKITKDQMEAILSRIKPTLDVAEAAKDADIVVEVVIELMDLK"
     gene            430..972
                     /locus_tag="PLR47_07630"
     CDS             430..972
                     /locus_tag="PLR47_07630"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOG12853.1"
                     /translation="MCKNPAMHIYIDGYNLIRQSVRLRRFERHSLEAGRDALIDWLVL
                     YKSRKDHRITVVFDGWVSGSAREERDCRAGIFMVYSPKGVKADEVLKRIIASSDEEIL
                     VVSSDREIASYAIRRGKAALSSPEFESIVDRQIAAPGDEFMDGKEEEDDADGRPTPKK
                     GPSRRMSRAQKQTLSKIKKL"
     gene            complement(969..2315)
                     /gene="holA"
                     /locus_tag="PLR47_07635"
     CDS             complement(969..2315)
                     /gene="holA"
                     /locus_tag="PLR47_07635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOG12854.1"
                     /translation="MSLEKVLLDLKKGKVAPCYLLYGEEEYLIQEALGDILDLLVPAS
                     DRDFGLFFIDGSEADFDLLRDRLSTPSLLGGAKVVVVKNTEIFQSKENLGNLIQKIRE
                     NLGEHPDKAVRSFQSFLKLSGFAWEDLQGGGWQKITDEQWRRAVEDDPGEDRHKWLPR
                     IVEICAGRGMTSGGDADISDSFEKLLADGLPAGNCLILTAEAVDRRKKIFKVIDKKGV
                     ILHFGAIKGEAATRETLKSEARRLLQACGKSLTSGAWAALGKKTGFQLRPSLNELEKL
                     IVFAGEKPVIGEEDVEAVVEKTREDRIFDLTNALGEKNAPAALLALQGLLDQGEPPLM
                     ILSMVSIEIRMLLQASILVQSGKLPPATPGMEFPVFQKKIYPAVSAMAESLPGKDFLP
                     GKNPYVIYKALGNCRRFSYPVLLGYLEDLVEMDRSMKSSATDPRILLERFLIKACA"
     gene            complement(2349..2999)
                     /locus_tag="PLR47_07640"
     CDS             complement(2349..2999)
                     /locus_tag="PLR47_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOG12855.1"
                     /translation="MEKQYLIDELSKEESWRIFRIMAEFVDSIEDLSRIHNGVTVFGS
                     ARVKPEDPYYQMAEKLGRLLVQNGFSVITGGGPGIMEAANKGAAEAGGQSVGMNIKLP
                     FEQKPNPYANLQIDYKYFFIRKVMFVKYAVAYIIMPGGYGTMDEFFEALTLIQTKRVK
                     SFPVIVMGREYWQGLLDWLKSVMLHKNMILPFDVEMIQIMDEPEEVVKHIQKYVIV"
     gene            complement(3135..3326)
                     /gene="rpsU"
                     /locus_tag="PLR47_07645"
     CDS             complement(3135..3326)
                     /gene="rpsU"
                     /locus_tag="PLR47_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOG12856.1"
                     /translation="MEVKVIDNDVEKALKILKNKLSKSGLFKELKVRRAYEKPSVKKK
                     RKTIEARRRLAKVQRRRNN"
     gene            complement(3689..4498)
                     /locus_tag="PLR47_07650"
     CDS             complement(3689..4498)
                     /locus_tag="PLR47_07650"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HOG12857.1"
                     /translation="MTWRLIPYRSYNAFQNMAIDEAIFRETLRNKRTPTLRFFGWRPA
                     AVSIGYFQDAQKEIHADGCRSAGVDLVRRITGGKAVYHRDEVTYSLAAGSREGLFPDD
                     IVRTYEMISSCLARGLAELGICASLAETGRKAGRAPCCFSEPAGKELVVDGRKICGSA
                     QIRSHGGFLQHGSLLMTFDPAATASLIRSPSPPEQLRDRVVGLNELIPVPVSAERLCE
                     ALRKGFCDGLGVDFAPGELTQEEWALGARLAKKYESAAWTLERKKEGFPAG"
BASE COUNT          959 a         1382 c         1184 g         1082 t
ORIGIN      
        1 cttcaggtcc atcagctcga tgaccacttc cacgacaatg tccgcgtctt tggcagcctc
       61 agcgacatcc agcgtcggct tgatccggct taaaatcgcc tccatctggt ctttggtgat
      121 ttttcctttt tcggcgtcgc ggcccaggtt ctttttgatg ttgttcatcc cgttgtcgat
      181 gaaacgctgt tcaatgtccc tgagggtcac ctcatagcct gccgccgcgc aaacctgcgc
      241 gatgccgttg cccatcaacc ccgcgcccaa cacgcatatt tttttaatgt ccataacaac
      301 ctccgattga tttattgtga tgcggttccc cgctttttca cgtgttccct gaagctacgc
      361 gacgctaaca gacggacaag ccttccgtca agaaaaatac cctctttttt cattgaatcc
      421 gccgcttcaa tgtgcaaaaa tcccgccatg catatttaca tcgacggcta taatctgatc
      481 cggcagtccg tccgcctgcg tcgcttcgag cgccacagtc tggaggccgg ccgcgacgcg
      541 ctcatcgact ggctggttct gtacaaaagc cggaaagacc accggatcac cgtcgtcttt
      601 gacggctggg taagcggctc cgcccgggaa gagcgggact gccgggccgg cattttcatg
      661 gtttactcgc ccaaaggcgt caaggccgac gaggtgctca aaagaatcat cgcctcttcg
      721 gacgaggaaa ttctggtggt ttcatccgac cgtgaaatcg cctcctatgc catccgccgg
      781 ggcaaggccg ccctgtcctc gccggagttt gaatccatcg tggaccggca gattgcggcg
      841 ccgggcgatg aattcatgga tggaaaagag gaagaggatg acgcagacgg tcgtccgacc
      901 cccaagaagg gcccgtcccg caggatgtcc cgggcacaga agcagaccct gtccaaaatc
      961 aaaaaacttt aggcgcaggc cttgattaaa aaccgctcca gcaggattcg cgggtcggtg
     1021 gccgacgatt tcatgctccg gtccatctcc accagatcct caagatagcc cagcagcacc
     1081 ggataggaaa accggcggca gttgccgagg gccttgtaaa tcacgtaggg attcttgccg
     1141 ggcagaaaat ctttgccggg caggctctcg gccatcgccg acacggcggg atagattttt
     1201 ttctgaaaca cgggaaattc catgcccggc gtcgcggggg gcagtttgcc ggattgcacc
     1261 aggatggacg cctgcaaaag cattcgaatc tcaatgctga ccatggacag gatcatcaaa
     1321 ggaggctcgc cctgatccag caggccctgc agcgccagaa gcgccgcggg cgcgtttttt
     1381 tcgccgaggg cgttggtcag atcaaaaatc ctgtcttccc tggttttttc aacgacggct
     1441 tcaacgtcct cttcgccgat cacgggtttt tcccccgcaa agacaatgag tttctccagt
     1501 tcattcaggg aaggacgaag ctggaatccc gtctttttcc ccagcgcggc ccaggcgccc
     1561 gacgtcagac tttttccgca ggcctgaagc agcctcctcg cttcgctttt gagcgtttcg
     1621 cgcgtggcgg cttccccctt gatggcgccg aaatgcagga tcacgccctt tttgtcgatg
     1681 accttgaaga tcttttttct tcggtccacc gcttccgcgg tcagaatcag gcaattcccg
     1741 gcgggaagcc cgtcagcaag aagcttttca aacgagtcgg agatgtccgc gtcgccgccg
     1801 gacgtcatgc cacggccggc gcagatttca acaatccggg gcagccactt gtgccggtcc
     1861 tctcccgggt catcctccac ggccctgcgc cactgctcgt ctgtgatttt ttgccagccc
     1921 ccgccctgca gatcctccca ggcaaacccc gacagcttta aaaacgactg gaaagaccgg
     1981 acggctttat ccgggtgctc gcctaagttc tcgcggattt tttgaatcag atttcccaga
     2041 ttttctttgg attgaaatat ttccgtgttt ttgaccacaa ccactttggc cccgcccaga
     2101 agcgatgggg ttgaaagacg gtcccgaaga agatcgaaat ccgcctcgct tccgtctata
     2161 aaaaaaagtc caaaatcccg gtcggacgcg ggcacgagaa ggtccagaat atcgccaagc
     2221 gcttcctgaa tcaggtattc ctcctccccg taaagaagat aacagggagc aactttacct
     2281 tttttgagat ccagcaggac tttttccagg gacatcaacg gctctcccgg cgggaaaacg
     2341 tgcggcgtct atacaatgac gtatttctgg atgtgtttga caacctcttc cggctcatcc
     2401 atgatttgaa tcatttcgac atcaaagggc aggatcatgt ttttgtgcag catgacgctt
     2461 ttcagccagt ccagaagacc ctgccagtac tcgcggccca tcacgatgac gggaaagctt
     2521 ttcactctct tggtctgaat cagcgtgagg gcttcaaaaa actcgtccat cgtgccatag
     2581 ccgcccggca tgatgatata ggcaacggca tacttgacaa acatcacctt gcggatgaag
     2641 aaatatttat aatcgatttg cagattggcg tagggattgg gcttttgttc aaagggcagc
     2701 ttgatgttca tccccacgga ctggccgccg gcctcggccg cgcccttgtt cgccgcttcc
     2761 atgatgccgg gaccgccgcc ggtgatcacg gagaatccgt tttgcaccag aagccttccc
     2821 agtttttccg ccatttgata ataggggtct tccggcttga cgcgggccga accgaaaacg
     2881 gtcacgccgt tgtgaatccg ggaaaggtcc tcgatcgaat ccacgaattc cgccatgatg
     2941 cgaaaaatcc gccaggactc ttccttggac aattcgtcga tgaggtattg cttttccacg
     3001 ctgcctgctc cttcaggatg ccgcccgtcc cgaataacca ttcgtctgca cgccggggcg
     3061 ccgaacgccg gactcaatgg gatcggaccg tcagcctgtt taaggaagag cggattgaag
     3121 aatccgcggt ttttttaatt gttgcggcgg cgctgtacct ttgccagtct gcggcgcgct
     3181 tcaatggttt ttcttttctt cttgaccgat ggcttttcat aagcccggcg gaccttcagc
     3241 tccttgaaca atccgctttt cgacagcttg tttttgagaa tcttaagcgc tttctccacg
     3301 tcgttgtcaa tcacttttac ttccaatgct ttacctcctc ataaatattg aatgtcatcc
     3361 ttaaatgggt gtgtcatctt gagatagatg ctggtcatcc cgagctgagt gtcgtgtcat
     3421 cctgagcttg tcgaaggatg acaccccttc gccttttaaa cgcaaaaaaa gggcagtccg
     3481 gcacacggtc tgtgccgaca accaccctca attttccaat cggttttaaa tcacgctccc
     3541 tgcccgaaaa gcccttttaa aaagctttcc cgcggttgtt gcccgcgtca aatatgcccc
     3601 gcggcacggc ttttttattt tttgcccgcc tcaggaagta tgcgtatatt cgatccccgt
     3661 gaaaattgca acaaaaataa cggcctcttt aaccggccgg aaagccttcc tttttgcgct
     3721 cgagcgtcca ggcggcgctt tcgtattttt tggcgagccg cgcgccaagc gcccattcct
     3781 cctgcgtcag ctcccccggg gcaaaatcga cgcccagccc gtcgcaaaaa ccctttcgca
     3841 gggcctcgca gagcctctcc gcgcttacgg gaacgggaat cagctcgttt aagccgacca
     3901 cgcggtctct caattgctcc ggcggcgaag gcgaccggat cagagaggcg gtcgcggccg
     3961 gatcaaacgt catcaggagc gatccgtgct gcagaaagcc accgtgcgac cggatttgcg
     4021 cgctgccgca gattttgcgg ccgtccacca ccagctcctt gcctgccggc tcggaaaagc
     4081 agcagggcgc ccttccggct ttccggccgg tttcggccag actcgcgcaa atgcccagtt
     4141 cggcgaggcc tcgcgccaga caggaactga tcatttcata cgtccggaca atgtcgtcgg
     4201 gaaacagacc ctcccggctt ccggccgcca gcgaataggt gacttcatcg cggtgataaa
     4261 cggcttttcc gccggtgatt ctgcggacca gatccactcc ggccgaccgg cacccgtctg
     4321 catggatttc tttttgcgcg tcctggaaat agccgatgga aaccgccgcc ggacgccagc
     4381 cgaaaaaacg cagtgtcggc gttctcttgt tcctcagggt ttcccggaat atggcctcgt
     4441 cgatggccat gttctgaaag gcgttatagg atcggtacgg gatcagtcgc caggtcatag
     4501 gagggcttca gtccgcttcc tgctgcagaa tgtaatcatg atagcctgcg gcatccagaa
     4561 gatcatcgag ctgctccatg tcgtccatct cgatcaccac cagccat
//
LOCUS       DAOSIM010000167         4537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_16871_length_4537_cov_8.865685, whole genome shotgun sequence.
ACCESSION   DAOSIM010000167 DAOSIM010000000
VERSION     DAOSIM010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16871_length_4537_cov_8.865685"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PLR47_07655"
     CDS             complement(<1..589)
                     /locus_tag="PLR47_07655"
                     /inference="COORDINATES: protein motif:HMM:NF015523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HOG12858.1"
                     /translation="MKLLKIYFLLTLTVMALAAGCAKKEAFRPEPPPPEKALVPADAR
                     RMDFVDDLDAESLLLAIDRSLRYYDGAGKNQTFQLVDRKVSAPRMKETLIAFREILAS
                     DIPVEQKKKRIAEEFLLLRAAGENGDGVVLFTGYYEPLLEGSLTKTEKYKYPLYRPPP
                     DIVVEKISKHETRISRREDGRLVPYYSRREIDVDGV"
     gene            complement(586..1044)
                     /locus_tag="PLR47_07660"
     CDS             complement(586..1044)
                     /locus_tag="PLR47_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOG12859.1"
                     /translation="MLRKARIGDVKTIHRLINVSAAKGEMLPRSLMDIYNSVRDFIVY
                     FDEAEQKIVGICAMNIIWENLAEIRSLYVDDAFRRKGIGRKLVEFCISDAITLELFRI
                     FTLTYKKEFFAGVGFKEVDRSILPEKVWSDCFRCPKFPDYCDETAMIIEL"
     gene            complement(1019..>2771)
                     /gene="recN"
                     /locus_tag="PLR47_07665"
     CDS             complement(1019..>2771)
                     /gene="recN"
                     /locus_tag="PLR47_07665"
                     /inference="COORDINATES: protein motif:HMM:NF008121.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOG12860.1"
                     /translation="VCLQALGKGKMLRDLSIANFAIIDELRVSFEEGLNIISGETGAG
                     KSILIGAVSLLLGDRAAADMIRTQTDTATVEALFDIQGNRALQEKLARMGFADGDDLV
                     IRRVISRSGKNKAQINGQMATLANLAAISESLINICGQHEHQVILFSENHIDILDAFG
                     GILDARGEYAAVYDRYQKCLGRMAKLEDLRRHRAEKADWIRFQLAEINDLNPVAGEDV
                     LLSDEKKVLANAQKLADWANRAYALLYADAGSINEKLKEALSQIQEIQKIDAGLNLRP
                     ADVEASYAVLQEAALTLRDYKQKLVFDPERLAAIDERQDAMGRLKRKHGQTLDAVLKR
                     KREMEEELQDVSGAEEELARLGKEKDAVAGELREKALELSKMRQRAAQSLERAVDREI
                     RDLNMPHASFAVKFLRRCSRDENTCGPKGGDEIEFYLTANAGEEPKALHKIASGGELS
                     RIVLALKNVLTRIGSVDTVVFDEVDSGIGGATAEIVGRKLKTLSAHHQVICITHLPQI
                     ASFGGPHLRVSKQVAGGRTSTVVEKIDDAQKIEEIGRMLGGVDVTETARDHARELLAA
                     AGADEGGSRHAKKSAHR"
     assembly_gap    2772..2837
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2838..3554)
                     /locus_tag="PLR47_07670"
     CDS             complement(<2838..3554)
                     /locus_tag="PLR47_07670"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOG12861.1"
                     /translation="MLSCQDIPLQEYLYKSKSLHSSPGKLSIDSGFFLCYETSSFLEP
                     VTMKIKAARADLIAVLGGDGTMLRTARYVAQHPVPIVGINMGTFGYLTEVNLNETYAA
                     FELILKGDFLTEKRMMLDAKIRRGNKIIGSGMVLNDVVINRGNLSRIVELETSVNGHY
                     LTTYKSDGLIISTPTGSTAYSLSAGGPIVVPGKELIIINPICPHTLTNRPIIFPENSD
                     LQITLWSKESGATVTLDGQES"
     gene            3653..>4537
                     /gene="rdgC"
                     /locus_tag="PLR47_07675"
     CDS             3653..>4537
                     /gene="rdgC"
                     /locus_tag="PLR47_07675"
                     /inference="COORDINATES: protein motif:HMM:NF016281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HOG12862.1"
                     /translation="MGLIKGTFTFMQFWVDGKLPQAFTTFIHSRLRGNAFREARNSTE
                     EKRMGWVSALDVLDADFEKTHYALGDYLIFSLRIDRKVIPPKLMKVRLMEEQRRFLAE
                     HKQTRIGKAMNEGIKEKVRLDIMTKSDPVPSFYDVLWAVGQNKVYFSSLSDKVADDFV
                     DLFKKTFSLGLKRTAPHEHPLVMKHKEKGGVPVEDLSLIGREFLTWLWFKSEERNGAI
                     ALGKTDEVQLHLLKRIALEAGEGEYSQGVVCSGLHAELKEGKEAIRQGKKVKEANLQL
                     QRDRDEWEFNFKADAFYFQ"
BASE COUNT          965 a         1297 c         1203 g         1006 t
ORIGIN      
        1 atacgccgtc cacatcgatt tcccggcggg agtaataggg aaccagccgg ccgtcttcac
       61 ggcggctgat tctggtttca tgtttggaaa tcttttccac gacgatgtcc ggcggcgggc
      121 ggtagagcgg atatttgtat ttctccgtct tcgtcagaga gccttccaaa agcggctcat
      181 agtagccggt aaacaggacg acgccgtccc cgttttcccc cgccgcccga agcaggagaa
      241 attcttccgc aatccgtttt ttcttctgct caacgggaat atcggatgcc agaatttcgc
      301 gaaaagcgat cagcgtttcc ttcatccgcg gcgcgctcac cttacggtcc accagctgaa
      361 aggtctgatt ttttccggca ccgtcgtaat agcgcagact gcggtcaatc gccagcagaa
      421 gggattcggc gtcgaggtcg tcgacaaagt ccatccgccg ggcatccgcc ggaaccagcg
      481 ccttctcggg cgggggcggc tccggcctga atgcctcttt ttttgcgcag ccggccgcca
      541 gggccatgac cgtcagcgtc aggagaaaat agatcttcag aagcttcata attcgatgat
      601 catcgcggtt tcgtcgcagt agtcgggaaa tttggggcag cggaaacagt ccgaccagac
      661 cttctccggc aaaatggaac ggtccacttc cttaaagcct actccggcaa agaattcttt
      721 cttgtacgtc agggtaaaaa tgcgaaacag ctccagggtg atggcgtcgg aaatgcagaa
      781 ttcaaccagc tttcggccga tgccttttct ccggaaggcg tcgtccacat aaagagaacg
      841 gatttcggcc aggttttccc agatgatgtt catcgcgcag attcccacga tcttctgctc
      901 ggcttcatca aaatacacga tgaagtcccg aacggaatta tagatgtcca tcagggaacg
      961 gggcagcatc tcccctttcg ccgccgacac attgatcagc cggtgaatgg ttttgacgtc
     1021 accgatgcgc gcttttctta gcatgtctgc ttcctccttc gtccgccccc gcggcggcca
     1081 gcaattcgcg ggcatggtcc ctcgcggttt cggtcacatc cacgccgccc agcattcggc
     1141 cgatttcctc gattttctgc gcgtcgtcga ttttttccac gaccgtggac gtccggccgc
     1201 cggcgacctg cttgctgacc cgcagatgag ggccgccaaa gctggcaatc tgcggcagat
     1261 gcgtgatgca gatcacctga tgatgcgccg acaacgtctt caacttgcgg ccgacaatct
     1321 ccgcggtggc gccgccgatg ccggaatcca cttcatcaaa cacaacggtg tctaccgaac
     1381 cgatccgcgt caaaacattc ttcagcgcca gcacgatgcg cgacaactcg ccgccggacg
     1441 caattttatg gagcgcctta ggctcctccc cggcattggc cgtcagataa aattcaatct
     1501 catcgccgcc cttcgggccg caggtgtttt cgtccctgga acagcgacgc agaaatttca
     1561 cggcaaagga ggcgtgaggc atgttcagat cgcggatttc gcggtccacc gcccgctcca
     1621 ggctttgcgc ggcccgttgc ctcatcttcg aaagctcgag cgccttttcg cgaagctctc
     1681 ccgctaccgc atctttttcc ttcccgagcc gcgccagctc ttcctccgcg ccggagacgt
     1741 cctgcagctc ttcttccatt tcccgcttcc gtttcaatac ggcgtccagc gtctggccat
     1801 gcttgcgctt gagccggccc atggcgtcct gccgttcatc gatcgccgcc agccgttcgg
     1861 gatcaaaaac cagtttttgc ttataatcac gaagcgtcag cgccgcttcc tgcaacacgg
     1921 cgtagctcgc ttccacatcg gccggccgaa gattcaaccc tgcatcgatc ttttgaattt
     1981 cctgaatctg cgagagcgct tccttgagct tttcattgat agagcccgca tccgcgtaaa
     2041 gcagcgcgta agcccggttc gcccagtccg caagcttctg cgcattggcc agtactttct
     2101 tttcatcgga aagcagaaca tcttcgcccg cgacgggatt caggtcgttg atttccgcaa
     2161 gctgaaaccg aatccaatcg gctttttccg cgcggtggcg gcgcagatcc tccagcttcg
     2221 ccatccggcc gaggcacttc tgatagcggt cataaacggc cgcgtattcc ccgcgggcgt
     2281 ccagaatgcc gccgaaagcg tcgagaatgt cgatgtgatt ttccgaaaag agaatcacct
     2341 gatgttcgtg ctgcccgcag atattgatca gcgattcgct gatcgcggcc agattcgcca
     2401 gcgttgccat ctgtccgttg atttgcgctt tgttcttgcc ggagcgcgaa atgacccggc
     2461 ggatcacgag gtcgtccccg tcggcaaacc ccatgcgggc cagcttctcc tgaagcgccc
     2521 ggttgccctg aatatcaaag agcgcctcca cggtcgccgt gtccgtttgc gtccggatca
     2581 tgtccgccgc ggcccggtcg cccaaaagca ggctgaccgc cccgatcaga atggacttgc
     2641 ccgcgccggt ctctcccgaa ataatgttca gaccttcttc aaacgaaacg cgcagttcgt
     2701 cgatgatggc aaaattagca atgctcagat cacgaagcat ttttcctttt cccagcgcct
     2761 gaaggcagac tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnngga ttcctgtccg tccagcgtca cggtggcgcc gctttccttg
     2881 gaccaaagcg tgatctgcag gtcggaattt tcggggaaaa taatcggccg gttggtcagc
     2941 gtgtggggac agatcggatt aatgatgatc aattctttgc cgggaacaac aatcgggcct
     3001 ccggcggaaa gcgaataggc cgtcgagccc gtgggggttg aaatgatcag accgtcggat
     3061 ttgtaggttg tgagataatg gccgttaacg gacgtttcca gctcgacgat gcgggaaaga
     3121 ttgccgcggt tgatcaccac gtcgttcaac accatgcccg aaccgatgat tttgttgccc
     3181 cggcggattt tcgcatccag catcatccgt ttttccgtca ggaaatcgcc cttgagaatc
     3241 agttcgaaag ccgcgtacgt ctcgttgaga ttgacttccg tgagataccc gaacgtcccc
     3301 atgttgatgc cgacgatggg cacggggtgc tgcgcgacat agcgggcggt gcgcagcatc
     3361 gtgccgtcgc cgcccagcac ggcgatcaga tccgctctgg cggccttaat tttcatggtg
     3421 acgggctcca aaaaagacga ggtttcgtaa cataaaaaga agccgctgtc tatggataat
     3481 tttccgggag atgaatgcaa actctttgat ttataaagat attcttgaag cggaatatct
     3541 tgacaggaca acatcccata aggtacggtg cgcatgctca cgcgggatta aaaccagcgg
     3601 ggcgtcataa aaccatgcca acaataacct tggctttttt ggagtaaagc atatgggatt
     3661 gatcaagggc acatttacat tcatgcagtt ttgggtggac ggtaaactgc cgcaggcctt
     3721 tacaacgttt atccacagcc ggcttcgggg caacgcgttt cgtgaagcgc gcaattccac
     3781 cgaagaaaag cggatgggtt gggtgtcggc gctggacgtc ctggacgccg attttgaaaa
     3841 aacacattac gcgctgggtg attatctgat cttttccctg cgcatcgacc gcaaggtgat
     3901 cccgccgaaa ttgatgaaag tccgcctgat ggaagaacag aggcggtttc tggccgagca
     3961 caaacaaacc cgaatcggca aggccatgaa tgaagggatc aaagaaaagg tccggctgga
     4021 catcatgacc aaaagcgacc ctgtgccctc tttttacgac gttctgtggg ccgtagggca
     4081 aaacaaggta tatttttcct ccctctccga caaggtcgcg gatgattttg tcgatttatt
     4141 caaaaaaact ttttcgctgg gcctgaagcg aacggccccc catgaacatc cgctggtgat
     4201 gaagcacaaa gaaaaaggcg gcgtccccgt ggaggatctg tctctcatcg gacgggaatt
     4261 tctcacctgg ctgtggttca agtccgaaga aagaaacggc gccatcgccc ttggcaaaac
     4321 cgacgaggtt cagttgcacc tgctcaagcg gatcgccctg gaagcgggcg agggggaata
     4381 ttcgcagggc gttgtctgct ccggtctgca cgccgaactc aaggaaggca aggaggccat
     4441 ccgccagggc aaaaaagtga aagaggcgaa ccttcagctc caacgcgacc gggatgaatg
     4501 ggaattcaat ttcaaggccg acgcctttta cttccag
//
LOCUS       DAOSIM010000168         4534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_16886_length_4534_cov_7.743693, whole genome shotgun sequence.
ACCESSION   DAOSIM010000168 DAOSIM010000000
VERSION     DAOSIM010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16886_length_4534_cov_7.743693"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PLR47_07680"
     CDS             complement(<1..310)
                     /locus_tag="PLR47_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOG12863.1"
                     /translation="MKFKKNILGIGAGYVGGPSMVMIASKCPDYRVTVVDVNPERIAA
                     WNSDRLPVYEPGLETIVKKTRGRNLFFSTGIEKGIQENDIIFVCVNTPTKTFGAGAGM
                     A"
     gene            complement(334..1275)
                     /locus_tag="PLR47_07685"
     CDS             complement(334..1275)
                     /locus_tag="PLR47_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG12864.1"
                     /translation="MKRILITGGAGFLGSHLCERLCGEGHDVICLDNFFTGSKNNISH
                     LLGQTHFELIRHDIINPIYLEVDEIYNLACPASPVHYQFNPIKTIKTNVMGAINVLGI
                     AKRTRAKILQASTSEVYGDPEVHPQHESYWGRVNPIGIRSCYDEGKRAAECLMMDYRR
                     QNAVRTTIARIFNTYGPRMAPNDGRVVSNFILQALQNRDITVYGDGSHTRSFCHASDL
                     IDGLIRLMEAPDDFYGPVNLGNPAEFTILELARLVIDLTGSRSKIVFQPLPSDDPAQR
                     KPDIALAREKLGWQPSVSLEQGLEDTIRYFRDILKTS"
     gene            1376..2230
                     /gene="rsmI"
                     /locus_tag="PLR47_07690"
     CDS             1376..2230
                     /gene="rsmI"
                     /locus_tag="PLR47_07690"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOG12865.1"
                     /translation="MDREARGVLYMVATPIGNLEDITLRALRVLKEVDLVAAEDTRRS
                     KILLSAHGIKTPLRSLHEHNEKERSAEILSRLQNGKSVAYVSDAGTPCVSDPGRHLVR
                     AARDNGIRVVPVPGACALPAALSVCGFQAEPFLFCGFLPPRSNRRRRFLESIMEEECS
                     IIFYESPARIRETLEDALDILGDRQVMAARELTKVFEEIREGKISELLAAWPGAGVKG
                     EFTVMLQGAAREPAVLSEREIGEQLLKILSEKGVSLRDAVAEAARRTQMPRKKIYDAA
                     LRIRREFP"
     gene            2412..3008
                     /locus_tag="PLR47_07695"
     CDS             2412..3008
                     /locus_tag="PLR47_07695"
                     /inference="COORDINATES: protein motif:HMM:NF017459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 family lipoprotein"
                     /protein_id="HOG12866.1"
                     /translation="MKIRPVFFALIIALALCLCACSSRGPDTLKNDAGTKAVNRIALL
                     PVENQTGDARVPKMLRLKLSEELRFKGYPQVDIEVIDGRLAAMAGAGEPGKTAIAPGV
                     VREMLGADAAMYCSLMESKVSKKIFYTPVTVSVRCELRSTATGETLWNAEGKATGRSF
                     DLRSKTLDLKALGDLEAVLEKVVDEVMESLPYGPKLSG"
     gene            3074..3841
                     /locus_tag="PLR47_07700"
     CDS             3074..3841
                     /locus_tag="PLR47_07700"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOG12867.1"
                     /translation="MREKTKIIVAVIMTLMLAAAAQAQSPVTVKMTKGRAQITSLKGK
                     VSMVCPGGKEAFYLKTYDFILPGCEAATGPDSRVEMVLPDRSVVRFSEKTKFKLVQAD
                     IGARGSRSVGISVTLGKIWTNVRKSLVGGSDRFEISCQNAVAGVRGTVYRMDVEKDQS
                     AVVKVYDGEVSVAGVSPAQPKAPVAFGPPKPVAGPTVIEGPKPVSMEQWVYIVRSMQQ
                     IQITVDGQAQKPRDFTQEEDMDDWVKWNQERDKQYGQ"
     gene            3863..4420
                     /locus_tag="PLR47_07705"
     CDS             3863..4420
                     /locus_tag="PLR47_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOG12868.1"
                     /translation="MNIPNFLSLLRIILVPVFVIFLIQAEYDRALITFVVAGLTDALD
                     GTLARLLKCQTVLGAYLDPLADKLLLATSFIMLAVIGLIPAWLAVVVISRDLIILLGI
                     AIFSLMSIAYEIKPAIISKVTTALQVGTIFFALLNKALPQDFGTFWIAVLFWATALFT
                     FGSGVVYILRGIRILNKSAPEETKK"
BASE COUNT          936 a         1197 c         1407 g          994 t
ORIGIN      
        1 cggccatgcc ggcgcccgcg ccgaaggttt tggtcggcgt gttgacgcag acgaagatga
       61 tgtcgttttc ctgaattcct ttttcgatgc cggtggagaa gaagagattc cgcccgcgcg
      121 tttttttgac gatggtctcg aggccgggtt cgtaaacggg aagccgatcc gagttccagg
      181 ccgcgatgcg ctcgggattg acgtccacga cggtcacacg gtagtccggg catttggagg
      241 cgatcatcac catcgagggg cctccgacgt agcctgcgcc gatgccgaga atgttctttt
      301 tgaatttcat atgtttgttt tcctttgccg gttctaggat gttttcagaa tatccctgaa
      361 ataccggatc gtgtcttcca gtccctgctc cagggaaacg gagggctgcc agcccagttt
      421 ttcgcgcgcc agggcgatgt cgggtttgcg ctgcgccgga tcatcggacg gcagcggctg
      481 aaagacgatc ttcgatcggc tgcccgtcag atcgatcacc aggcgcgcca gctccaggat
      541 ggtgaattcc gcgggattgc cgaggttgac cggcccgtag aaatcatcag gcgcttccat
      601 cagccggatg agtccgtcga tcagatcgct tgcgtggcag aaggagcgcg tgtggcttcc
      661 gtcgccgtaa acggtgatgt cccggttctg cagggcctga aggatgaagt tgctgacgac
      721 tcggccgtcg tttggcgcca tgcgcggtcc gtaggtgttg aagatgcggg cgatggtcgt
      781 gcggacggcg ttttgccggc gatagtccat catcaggcat tccgcggctc ttttgccctc
      841 gtcgtagcag cttcgaatgc cgatgggatt gacgcggccc cagtaggatt catgctgcgg
      901 gtgtacttcc ggatcgccat agacttccga ggtggaggcc tgaagaattt tcgcccgcgt
      961 tcgtttggcg atgcccagaa cgttgatggc gcccatgacg ttcgttttga tggttttgat
     1021 cgggttgaac tggtagtgaa cgggcgacgc cggacaggcg aggttgtaga tttcgtccac
     1081 ctcaagatag atggggttga tgatgtcgtg ccggatcagt tcgaaatgcg tctgtcccag
     1141 caggtgcgag atgttgtttt tactgccggt aaaaaagttg tccaggcaga tcacatcatg
     1201 tccttcgccg caaagccgct cgcacagatg ggagcccaaa aaacccgctc cgcccgtaat
     1261 caggattcgc ttcatatccg gttggctgtt cttccctgta gtaatttatt ttcgtctctg
     1321 ctaataaagc cgaagggctc ggatgtcaac cataaaaagt cagaggggcg gcaaaatgga
     1381 cagggaagcg cggggagttc tttacatggt ggccacgccg atcggcaatc tggaggatat
     1441 tacccttcgg gcgctgcgtg tgctgaaaga ggttgatctc gttgccgcgg aggacacccg
     1501 ccgctccaaa atcctgcttt ccgcgcatgg gatcaaaacg ccgctccgca gtctgcatga
     1561 gcacaacgag aaggagcgaa gcgccgaaat tctatccagg ctccaaaacg gaaagagcgt
     1621 cgcctacgtc tcggatgccg gaactccctg cgtctccgat cccggccggc atctcgtgcg
     1681 ggcggcccgc gacaacggca ttcgcgtggt tcccgttccg ggcgcctgcg ctttgcccgc
     1741 ggccctgtcg gtttgcggct ttcaggccga accatttttg ttctgcggct ttctcccccc
     1801 ccggtccaac cgccgccgcc gctttctgga gtccattatg gaagaggaat gctccatcat
     1861 tttctatgag tctccggcca gaatccgtga aacgctggaa gacgcgctgg acattctggg
     1921 cgaccggcag gtcatggcgg cgcgggaact gaccaaggtg tttgaagaga tccgggaggg
     1981 caagatttcc gaactgctgg ccgcatggcc cggcgcgggc gtcaaggggg aatttacggt
     2041 gatgcttcag ggcgccgccc gcgagcctgc ggttctttcg gagcgggaga tcggggaaca
     2101 gcttttgaaa atcctttcgg agaaaggcgt ttcgctgcgg gatgccgttg cggaagcggc
     2161 gcgccgaacc caaatgccca gaaaaaaaat atacgatgcg gcgcttcgaa tccgccggga
     2221 atttccgtaa gggagcgggg ccgccgcggt ttacatattg cttgaagagt tgccgtcact
     2281 atgctaatcc catgtcgaaa tgaaagcaat tccccgcggt cgggggagag ccttccgctt
     2341 cgggggagaa gtttttttgc gacccccggc ggcaaacaaa ttcgccgccc gattgaaaaa
     2401 agggagttgt catgaaaatc cgtccggttt ttttcgctct gatcattgcg ctggccctgt
     2461 gcctttgcgc ctgttcgtcg cgcggcccgg atacgctgaa aaatgatgcc ggcacaaaag
     2521 cggtgaaccg gattgccctg ctgccggtgg agaatcaaac cggagacgcc agggtgccga
     2581 aaatgctccg tctcaaattg tcggaagaac ttcgctttaa aggctatccc caggtggata
     2641 tcgaggtcat tgacggccgt ctggccgcca tggcgggcgc cggcgaaccg gggaagaccg
     2701 cgattgctcc aggggttgtc cgggaaatgc tgggcgcgga cgccgccatg tactgcagcc
     2761 tgatggaaag caaagtttcc aaaaagattt tttacacgcc ggtcaccgtg tcggtgcgtt
     2821 gcgaattgcg cagcacggca accggcgaga ccctgtggaa cgcagagggc aaggcgaccg
     2881 gcagaagctt tgatctcaga agcaaaacgc tggacctgaa agccctggga gatctggaag
     2941 cggttctgga aaaggtggtg gacgaggtga tggaatccct gccgtacgga ccgaaattga
     3001 gcggatagtc cgccgcggtc cgtccggaaa tgaggggtca aaccggtctc ggaaagatgc
     3061 aaaggagaaa cgcatgaggg aaaaaacgaa aatcatcgtg gctgtgatta tgacgctgat
     3121 gctcgccgcc gccgcgcagg cccagtcgcc ggtgaccgtc aaaatgacca aaggacgggc
     3181 tcagatcaca tcgttgaagg gaaaagtttc catggtctgt ccgggcggga aggaggcgtt
     3241 ttacctcaaa acctatgatt tcatcctgcc cgggtgcgaa gccgccacgg gcccggactc
     3301 cagggtggag atggttttgc ccgaccgaag cgttgtccgc ttctcggaga agacaaaatt
     3361 caaactggtc caggccgaca tcggcgcacg gggaagccgt tcggtcggca tttccgttac
     3421 gctcgggaaa atctggacaa acgtgcgcaa gtccctggtg ggcggaagcg accggtttga
     3481 aatctcctgc cagaatgccg tagccggggt tcgcggcact gtttaccgca tggatgtcga
     3541 aaaggaccag tccgcggtgg tcaaggttta cgacggcgaa gtcagcgtcg cgggtgtctc
     3601 cccggcgcag cctaaagcgc cggtcgcctt cgggcctccc aaaccggttg cagggccgac
     3661 ggtgattgaa gggccgaagc ccgtctcgat ggagcagtgg gtgtatatcg tccggtccat
     3721 gcagcagatt caaatcacgg tcgacggcca ggcccagaag ccgagggatt ttacgcaaga
     3781 agaggacatg gatgactggg tgaagtggaa ccaagagaga gataagcagt acggccaata
     3841 acgtcgggcg cagaatgtat agatgaatat ccctaacttt ctatcgcttt tgcggatcat
     3901 actggttccg gtgttcgtca tctttctgat tcaggcggaa tatgaccggg cgctgatcac
     3961 tttcgtcgtt gccggcttga cggacgcgct ggacggaacg ctggcccggc ttttgaaatg
     4021 ccagaccgtt ctgggcgcct atcttgaccc tcttgccgat aaacttcttt tggccacaag
     4081 ctttatcatg ctggccgtga tcggtctcat tccggcatgg ctggccgtgg tggtcatcag
     4141 ccgggacttg atcatcctgc tgggaattgc aatattctca ctgatgtcga ttgcttacga
     4201 aatcaagccg gcgattatca gcaaggtgac gacggccctg caggtgggca ccattttttt
     4261 tgccctgctg aacaaggctc tgccccagga ttttggaaca ttttggatcg ccgtgctttt
     4321 ctgggccacg gcgttgttca cctttggttc tggcgtcgtt tacattctgc gcgggatacg
     4381 gatactgaat aaatccgcac ccgaagagac gaaaaagtaa gatattatct tgacacgaaa
     4441 gcgttttgct gttaggtgac tcaccgcaaa agttgaagat aggcaagtag ctcaggggga
     4501 gagcgccatc ctgacgcggt ggatgtccag ggtt
//
LOCUS       DAOSIM010000169         4504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_17028_length_4504_cov_8.503034, whole genome shotgun sequence.
ACCESSION   DAOSIM010000169 DAOSIM010000000
VERSION     DAOSIM010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17028_length_4504_cov_8.503034"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLR47_07710"
     CDS             complement(<1..448)
                     /locus_tag="PLR47_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOG12869.1"
                     /translation="MSKTEDALKEAFAGESQANRKYLAFAAKADQEGFAQAARLFRAA
                     AEAETIHAHAHLKALKGIRSTKENLQEAVDGETHEFKSMYPAMIEAAKAEGHKEAERS
                     FIFANEVEKIHAGLYQQMLDNLDGAKETYGYYICPVCGYTAEKEAPE"
     gene            complement(482..2692)
                     /gene="katG"
                     /locus_tag="PLR47_07715"
     CDS             complement(482..2692)
                     /gene="katG"
                     /locus_tag="PLR47_07715"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011635.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HOG12870.1"
                     /translation="MAEESKCPVTGAMKKSISGRGASNRDWWPNQLNLKILHQHTQKS
                     NPMGEAFDYAKEFKKLDLKAVKKDLYALMTESQDWWPADWGHYGGLFIRMAWHSAGTY
                     RMGDGRGGAGSGSQRLAPLNSWPDNVNLDKARRLLWPIKQKYGKKISWADLMILAGNC
                     ALESMGFKTFGFAGGRVDVWEPEEDIYWGSEEEWLATSDKPKSRYTGERDLENPLASV
                     QMGLIYVNPEGPDGNPDPVASGRDVRETFARMAMNDEETVALVAGGHTFGKCHGAGPA
                     THVGPEPEAAPIEEQGLGWKSSFKSGKGIDTISSGIEGAWKPNPTNWDMGYLKVLFKY
                     EWELVKSPAGANQWLAKNVEEEDMVVDAHDPSRKRRPMMTTADLSLRYDPIYEPIARR
                     YSANPKQFADAFARAWFKLTHRDMGPRSRYLGPEVPKEVLIWQDPIPAVNHKLISKSD
                     AAFLKSKILASGLSVSGLVSTAWASASTFRGSDMRGGANGARIRLAPQKDWEVNEPER
                     LARVLKMLERIQKAFNKEQGGDKKVSLADLIVLAGCAGVEQAAKNAGYKINVPFSPGR
                     MDASQKQTDAASFGVLEPKADGFRNYQKARFTVLPEELLVDKAQLLTLTAPEMTVLIG
                     GLRVLGANFSGLPHGVFTSRPGALTCDFFVNLFDMGTEWKASVNDPGVFEGYDRKTGE
                     LKWTGTRVDLIFGSNSQLRAVAEVYACQDSPEKFLGDFVAAWNKVMNLDRFDLA"
     gene            complement(2814..3248)
                     /locus_tag="PLR47_07720"
     CDS             complement(2814..3248)
                     /locus_tag="PLR47_07720"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOG12871.1"
                     /translation="MRTNTKEDLIGQLKENGLKITPQRLAIIDALVENRYEHPGATLI
                     FNEARKKAGRVSLSTVYATLKEFSESGLIRQMEFDRLENRYDVDLSDHVNLICKRCGK
                     VVDYHIPAPLEPKNIVRKSGFVVTDTRIEFHGYCRDCLKRPD"
     gene            complement(3404..3934)
                     /locus_tag="PLR47_07725"
     CDS             complement(3404..3934)
                     /locus_tag="PLR47_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOG12872.1"
                     /translation="MSIDQILGLVTGVVFGFLMQKGRVIRFEKQVGALLIKDMTIFKF
                     MLSSILVGMVGIFILSDMGIITFSHKPMNVGAVVIGAILFGIGWAVMGFCPGTSVAAV
                     GEGRVHALFGIAGMLVGAAVFAEIYPFLQSTVMAWQDFGKISLPGALGISHWVLVPIF
                     WAATIGLFVVFEKNGL"
     gene            complement(3931..4473)
                     /locus_tag="PLR47_07730"
     CDS             complement(3931..4473)
                     /locus_tag="PLR47_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOG12873.1"
                     /translation="MRLKTKDGGWNPYFAGALVGVLAIVSVCATTELMGKTNYLGAST
                     TFVRAAGFVEQVFASEHVAQNAYFAATKIKVDWQFMLIIGIFLGSLISSLTDKSFLVE
                     KVPPIWKERFGSSMAKRGAGAFLGGIVAMIGARLADGCPSGHGLSGMMQLSASSFVAL
                     VLFFAAGALTAVLVYKRRAS"
BASE COUNT          923 a         1335 c         1185 g         1061 t
ORIGIN      
        1 tttccggcgc ttctttttcc gcggtatagc cgcagaccgg acagatataa tagccgtagg
       61 tttcttttgc cccgtccaga ttgtccagca tttgctgata aagtccggca tgaatctttt
      121 ccacctcgtt ggcgaaaata aatgagcgct ccgcctcctt gtggccttcc gcctttgccg
      181 cttcgatcat ggcaggatac atgcttttga actcatgcgt ttctccatcg accgcctcct
      241 gaaggttttc ctttgtggag cggatgccct ttaacgcctt caaatgggcg tgggcgtgaa
      301 tggtttccgc ctccgcggct gcccggaata gtcttgcggc ctgggcaaat ccctcctggt
      361 cggctttggc tgcaaacgcc agatattttc ggttcgcctg tgattctcct gcaaaagctt
      421 cctttaacgc atcttctgtt ttcgacatga ttttctcctt cattatattt tattgttaat
      481 atcacgccag gtcgaaacgg tccagattca tgactttgtt ccaggccgcc acaaaatcgc
      541 ccagaaactt ctccggggag tcctgacagg cgtaaacttc cgccaccgcc cgaagctgcg
      601 agttggaccc gaaaatcaga tcgacgcggg tgccggtcca tttgagctcg ccggtcttgc
      661 ggtcgtaccc ttcaaacaca cccggatcgt tcacggatgc cttccactcg gtgcccatgt
      721 cgaacaggtt gacgaagaag tcgcaggtga gcgcacccgg acgcgaggtg aaaacgccgt
      781 gcggcaggcc gctgaagttt gcgcccagca cgcgcaggcc gccgatgagc accgtcatct
      841 ccggcgcggt aagcgtgagc agttgcgcct tgtccaccag cagctcttcg ggcagcacgg
      901 tgaacctggc cttctgatag ttgcggaaac cgtcggcttt cggttccagc acgccaaacg
      961 aagccgcatc ggtctgtttt tgcgatgcgt ccatgcggcc gggcgagaag ggaacattta
     1021 ttttgtaccc ggcatttttc gccgcctgtt caacaccggc gcaaccggcc aaaacaatca
     1081 gatcggccag cgataccttc ttgtcgcccc cctgctcttt gttgaacgct ttctgaatgc
     1141 gctcgagcat tttgagcact ctggccagac gctccggttc gttcacttcc caatcctttt
     1201 gcggcgccag gcgaatgcgc gcgccgttgg cgccgccgcg catatcggag ccgcggaacg
     1261 tggaagcgga cgcccaggcc gtcgaaacca gtcccgaaac ggacaggccc gaagccagga
     1321 ttttgctctt gaggaaggcg gcgtccgatt tgctgatcag cttgtgattg acggcgggaa
     1381 tgggatcctg ccagattagc acttctttgg gcacttccgg gccgagataa cgcgaacgcg
     1441 ggcccatgtc gcggtgcgtc agcttgaacc aggcgcgggc gaaggcgtcg gcaaattgtt
     1501 tggggttggc cgaataacgc ctggcaatcg gttcatagat cgggtcgtag cgcagcgaga
     1561 ggtccgccgt ggtcatcatc gggcgacgct tcctggacgg atcgtgtgcg tccaccacca
     1621 tgtcttcttc ttccacattc ttggccagcc actgattggc gccggccggg cttttgacca
     1681 gttcccactc atatttgaag agcaccttca ggtagcccat atcccagttc gtcggattcg
     1741 gtttccaggc cccttcgatg ccgctggaaa tcgtgtcgat gcctttgccg cttttgaagc
     1801 tgcttttcca gccaagaccc tgctcttcga tgggagccgc ttccggttca ggtcccacgt
     1861 gggttgctgg ccctgcgccg tggcatttgc cgaaagtgtg tccgccggcg accagcgcga
     1921 cggtttcttc gtcgttcatg gccatacgcg caaaggtttc gcgcacgtcg cggccggagg
     1981 cgaccggatc gggattgccg tccggacctt cagggttcac gtagatcagc cccatctgca
     2041 cggaggcgag aggattttcg agatcgcgct ctcccgtgta gcggctcttg ggcttgtcgc
     2101 tggtggccag ccattcctct tcgctgcccc agtagatgtc ttcttccggc tcccagacgt
     2161 ccacgcgtcc gccggcgaat ccgaaggttt taaagcccat cgactccagg gcgcagttgc
     2221 cggccagaat catcagatcg gcccaggaga tttttttgcc gtatttttgc ttgatcggcc
     2281 acaaaagcct tctagcctta tcgaggttta cgttgtcggg ccaggagtta agcggcgcca
     2341 gacgctgaga cccggagccc gcgccgcccc ggccgtcgcc catgcggtag gtgcctgcgc
     2401 tgtgccaggc cattcgaatg aaaagtccgc cgtaatggcc ccagtcggcg ggccaccagt
     2461 cctgggattc ggtcatcagt gcgtagaggt ctttcttgac ggctttcagg tccagttttt
     2521 taaattcttt ggcgtagtca aacgcttcgc ccatcggatt gcttttctgt gtatgctgat
     2581 gaagaatttt gagattcaat tgattcggcc accagtcccg gtttgaggcg cctctgccgg
     2641 aaatggattt tttcatcgcg cccgtcaccg gacatttgct ttcttcggcc atttcatttc
     2701 ctcctttcct cactggttaa attggaatca ttctaagtag gaaacggtaa gacccttccc
     2761 ctcgcttcgc atgccatcgg tgttgcctcg tgcggaaccg cagaatcaaa aaatcagtcg
     2821 gggcgcttca aacaatcccg gcaatagccg tgaaactcga tccgggtgtc ggtaacgaca
     2881 aatcccgatt ttcggacaat attttttggc tcgagcggcg cgggaatgtg gtagtccacc
     2941 acctttccgc accgtttgca gatgagattc acatgatcgg agagatccac gtcatatcgg
     3001 ttctccagcc ggtcaaattc catttggcgg atcagcccgc tttcggaaaa ttccttgagc
     3061 gtcgcataca ccgtcgagag gctgacgcgc ccggcttttt tccgggcttc gttgaagatg
     3121 agcgtcgcgc cgggatgctc gtagcggttc tccaccagcg catcgataat cgccaggcgc
     3181 tgcggcgtaa ttttgagccc gttttctttt aactgaccga tcagatcttc tttggtattc
     3241 gttctcatat cggaatcatt ccagtttgga aaatcctacc ctgatccggc gcgtcctgtc
     3301 aagcggtttt tgcaaattgg aagaacgggc gatcaattga ttcccgtcca ccggcatacc
     3361 agccttcgat gccagtggac aggaacctga taacgttcag attttacaga ccatttttct
     3421 cgaacaccac aaacaggccg atcgttgccg cccagaagat cggcacaagc acccagtggg
     3481 aaatacccag agcgcccggc aggctgattt tgccgaagtc ctgccacgcc atgacggttg
     3541 attgaaggaa aggataaatc tcggcaaata ccgccgcacc gacgagcatt cccgcaatgc
     3601 cgaacaaagc gtgcacacgg ccttccccca cagccgcgac ggaagttccg gggcagaatc
     3661 ccatcaccgc ccagccgatg ccgaaaagaa ttgcaccgat aaccacagcg cccacattca
     3721 tcggtttgtg gctgaaagta atgattccca tatccgagag aatgaagatt cccaccatgc
     3781 cgaccagaat cgaggaaagc atgaatttga aaattgtcat gtcttttatc aaaagcgcgc
     3841 caacctgttt ttcaaaacgt atgacccgcc ctttctgcat cagaaatccg aacaccacac
     3901 cggtaaccaa acccaaaatt tgatcgatgc tcatgatgcc ctccttttgt ataccagcac
     3961 cgcggtcaga gcgccggccg caaaaaacag caccagcgcc acaaaagagc ttgccgacaa
     4021 ctgcatcatg ccgctgagtc cgtgtccgct gggacagcca tccgccagcc gcgcaccgat
     4081 catggccacg atgccgccca ggaaagcgcc tgcgcctctt ttggccatgg aggagccgaa
     4141 gcgttccttc cagatgggcg gcactttttc caccagaaaa cttttatccg tgagagaaga
     4201 gatcagcgag cccaggaaga ttccgatgat gagcatgaac tgccagtcca ctttgatttt
     4261 tgtggcggca aaataagcgt tttgagcaac atgctccgag gcgaaaacct gctccacaaa
     4321 accggcggcg cggacaaaag tggtggacgc gcccagataa ttggttttgc ccatcagctc
     4381 cgtcgtggcg caaactgaaa caatcgccag gacgcccacc agcgctccgg caaagtaagg
     4441 attccatcct ccgtcttttg ttttaagtct catcgttgcc tcctttcgtt gattgaatta
     4501 gcca
//
LOCUS       DAOSIM010000170         4488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_17121_length_4488_cov_7.360929, whole genome shotgun sequence.
ACCESSION   DAOSIM010000170 DAOSIM010000000
VERSION     DAOSIM010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17121_length_4488_cov_7.360929"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PLR47_07735"
     CDS             complement(<1..330)
                     /locus_tag="PLR47_07735"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOG12874.1"
                     /translation="MAGKTYKGLMVRETAENQFTRAIEELSTDGLPAGDVLVKVLFSS
                     LNYKDVLSAGGNRGVTKKYPHTPGIDAAGIVEESASPNFKAGDEVIVTSYDLGMNTSG
                     GLGQYIRV"
     gene            complement(388..1512)
                     /locus_tag="PLR47_07740"
     CDS             complement(388..1512)
                     /locus_tag="PLR47_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12875.1"
                     /translation="MIKIGLLFLSFVFVFVVSGCGNYYKVVDPVSKKVYFTDSIDQKG
                     RGVIQFKDKLSKNVVTLPESEVMEITKDQYLAGAPVKAAQPIPYCTEDDADWCGPCWT
                     NPANTWCCKHRGGKDPNCGVDATTCKYCEGCHQGDDCINICAKPPKGAGGSTNIKITN
                     ATDKDVTIAFVTGASGGACKDVKKMISYQWVAQNTNWCKNPKQEGGPNAGYCTGIVPK
                     RSFVEVTRTGENALKCLTGSILLGGKLSCPEPTGFTQGEFTLNPTDTDTEAVDISLVN
                     GVNYALTVNLPGKAWKVQDGGAAVTSIGPNAGFDGDNNKNGIFPPGCTDCIVAVEGQI
                     PCPKITPNPKCQPKRICNIYRGGWTGGTVEYVISDLPKPK"
     gene            complement(1614..2729)
                     /gene="prfA"
                     /locus_tag="PLR47_07745"
     CDS             complement(1614..2729)
                     /gene="prfA"
                     /locus_tag="PLR47_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOG12876.1"
                     /translation="MGILLNKLHDIELRYRELEGMLSDAQMASRQGLYQKYAKEHSDL
                     SELVETIRRYEKIRTDLAENQGLLADSDEEVKALAKEEIPQLQQELAELEEKIKILLL
                     PKDPNDDKNVFLEIRAGTGGDEAGLFVGDLFRMYARYAESQGWKVEIVSSSPAGGMGG
                     FKEIIAQIEGRGAYSRLKYESGTHRVQRVPVTEAQGRIHTSAVTVAIMPEAEEVEVNI
                     DPNELRIDVYRSTGHGGQSVNTTDSAVRITHIPTGLVVTCQDEKSQLKNKAKALKVLR
                     SRLLDAMVQKQNAEQAQVRKNQVGSGDRSERIRTYNFPQGRITDHRINLTRYDLDSFI
                     NGNIGAVIDALASHHQAELLNKEGKGGKGDTLKKLSF"
     gene            complement(2896..3138)
                     /gene="rpmE"
                     /locus_tag="PLR47_07750"
     CDS             complement(2896..3138)
                     /gene="rpmE"
                     /locus_tag="PLR47_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014697411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOG12877.1"
                     /translation="MKAGIHPDYKPTTITCVCGNVIETRSTKKDIKIEICSNCHPFMT
                     GKQKILDTAGRVEKFKKKYAPKAETAPAAKKTTKKA"
     gene            complement(3245..4486)
                     /gene="rho"
                     /locus_tag="PLR47_07755"
     CDS             complement(3245..4486)
                     /gene="rho"
                     /locus_tag="PLR47_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOG12878.1"
                     /translation="MEDIKRLPISELNNLAKELEVPGASGMRRQDLIFAILQTQAEQN
                     GVISGSGVLEILPDGFGFLRAVDYNYLPSPDDIYISPSQIRRFNLRTGDTISGEVRPP
                     KEGEKYFALLKVDEVNFESPESARDKILFDNLTPLYPQEKLNLEFDPENFSTRTLDLF
                     APIGKGQRALIVSPPRAGKTVLLQDIAHSITANHKEVVLMVLLIDERPEEVTDMQRSV
                     KGEVISSTFDEPASRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVVPPS
                     GKVLSGGVDSNALHKPKRFFGAARNIENGGSLTIISTALIDTGSRMDEVIFEEFKGTG
                     NMELHLDRRIADRRIFPAFDLIRSGTRKEELLIPKANLNRVWILRRLLQEMNPVDAME
                     FIIGKIKKTETNQEFLDSMNS"
BASE COUNT          867 a         1174 c         1226 g         1221 t
ORIGIN      
        1 aacccggatg tactggccca gaccgccgga tgtgttcatg cccaggtcgt agctcgtgac
       61 gatgacttcg tcgccggctt tgaagttggg cgaagcgctt tcttcgacaa tgcccgccgc
      121 gtcgatgccg ggcgtgtggg gatatttttt cgtcacgccc cggttgccgc cggcggagag
      181 cacgtcctta taattcagcg atgaaaaaag aaccttgacc agaacatctc ccgccgggag
      241 cccgtcggtg gataattctt caatggccct tgtgaactgg ttttcggctg tttctctgac
      301 catcagccct ttgtacgttt tgcctgccat aatgacctcc tcggaatgga ttgaaaagag
      361 tggagtgctt gtctgccgac ccgtcaatca tttcggtttc ggcaaatcgc tgatcacata
      421 ctccacagtt ccacccgtcc agccgccgcg ataaatattg cagatgcgtt tcggctggca
      481 tttgggattg ggcgttattt tcggacaggg aatctgcccc tctaccgcca caatacaatc
      541 ggtgcatccg ggaggaaata ttccattctt attgttgtcc ccgtcaaagc ccgcattggg
      601 gccgatagag gtgacggcgg cgcccccgtc ctgaactttc catgccttgc ccggcaggtt
      661 gacggtcaag gcatagttca cgccgtttac cagactgata tccacggcct ccgtgtcggt
      721 atccgtcgga ttcagcgtga actctccctg tgtaaatcca gtgggctcgg gacatgacaa
      781 tttgccgccc agcaaaatcg agccggtcag acatttcagg gcattttccc cggtccgcgt
      841 cacttccaca aagcttcttt tgggaacgat ccccgtgcag tatcctgcat tagggccgcc
      901 ttcctgtttg gggtttttgc accaatttgt gttctgtgca acccattgat aggaaatcat
      961 ttttttgacg tccttgcatg cgcctcccga tgcgcccgtc acaaaggcga tcgtgacgtc
     1021 tttgtcggtt gcgttggtga tcttgatgtt tgtcgatcct ccggcgcctt tcgggggctt
     1081 ggcgcaaatg ttgatgcaat catcgccctg atggcaacct tcgcagtact tgcacgttgt
     1141 cgcatccacg ccgcaattcg ggtctttccc gcccctgtgc ttgcagcacc aggtgtttgc
     1201 cggattggtc cagcacggac cgcaccagtc cgcgtcgtct tcggtgcagt acggaatcgg
     1261 ctgcgccgct ttcacggggg cgccggccaa atactgatct tttgtaattt ccataacttc
     1321 cgattccggc aatgtcacca cattttttga taatttatct ttgaactgga tgacgcctct
     1381 ccctttttga tcgatgctgt ccgtgaaata gacttttttg gaaaccggat cgacaacctt
     1441 ataatagttg ccgcatccgg aaacaacaaa aacgaaaaca aacgacagaa acaataaccc
     1501 aattttaatc ataaatgcat ttccctccat aggggacacc cttaagtatt taagttaatc
     1561 cccttaattc ttttttatct ttacgctgca atcgacctga aatctgaccg ctgttagaaa
     1621 cttaatttct taagggtgtc ccctttacct cctttaccct ctttattgag cagttcggcc
     1681 tggtgatgtg aggcgagcgc gtcgattacg gcgccgatgt tgccgttgat gaagctgtcg
     1741 aggtcgtagc gggtgaggtt gatccggtgg tcggtgatcc gtccctgtgg aaagttgtag
     1801 gtgcggatgc gctcggagcg gtcgcccgac ccgacctggt ttttacgcac ttgggcctgc
     1861 tcggcgttct gcttttgcac catggcgtcc agaagacggg atcttaatac tttcaaggcc
     1921 ttggctttgt tcttgagctg ggatttttca tcctgacagg taacgacaag gcccgtcggg
     1981 atgtgcgtaa tgcggacggc ggaatccgtg gtgttgacgc tctggccgcc gtggccggtg
     2041 gagcggtaaa cgtcgatgcg aagttcgttt gggtcgatgt tcacctccac ttcttccgct
     2101 tccggcataa tggcgacggt gacggcggag gtgtgaatgc gcccctgcgc ttccgtgacg
     2161 ggcacccgct gaacgcgatg tgtgccgctt tcgtatttca ggcggctgta ggcgcccctg
     2221 ccttcgatct gggcgatgat ttccttgaag ccgcccatgc cgcccgccgg cgatgaactg
     2281 acgatttcca ccttccagcc ctgggattcc gcgtagcgcg cgtacatgcg gaacaaatcg
     2341 ccgacaaaca ggccggcttc gtcgccgccg gtgcccgccc ggatttcaag aaagacgttt
     2401 ttgtcgtcgt tggggtcttt gggcagaagc aggattttaa ttttttcttc cagttcggca
     2461 agttcctgct gaagctgcgg aatttcttct ttggccagcg cctttacctc ttcgtcgctg
     2521 tccgccagca gtccctggtt ttccgcaagg tctgtcctga ttttttcata tctgcggatc
     2581 gtttccacca gttcggagag atccgagtgc tctttggcgt atttctgata cagtccctgc
     2641 ctggaagcca tctgggcgtc ggaaagcatc ccttcgagct cccggtagcg gagttctatg
     2701 tcatgaagtt tgttgagtaa aatacccata tacttaattt atatcgttgt ttttaatgca
     2761 ggttgtgaag ggaaggcgca ttgatagccc ttccccatgt ctgtgaggac gcaactataa
     2821 atgccgggaa agttttgatc tgttgatcat gagggcaaag tccaaaagtg tccgcctttc
     2881 ggcagaaagt taagcctatg ctttttttgt tgttttcttt gcggccggag ctgtttcggc
     2941 tttcggcgca tatttctttt tgaatttttc cacgcgcccc gccgtgtcga gaattttctg
     3001 cttgccggtc ataaacggat ggcagttgga gcagatttcg atcttgatgt cttttttggt
     3061 cgagcgcgtt tcaatgacat tgccgcaaac acacgtgatg gtggttggct tgtagtccgg
     3121 atgaatccct gctttcattt tccttcttcc tcctgatcct ctatcggttg tttggataaa
     3181 ggtctctgta attttcagca gatgtgctgc ctataccata aactttgaaa attcaagata
     3241 attcttacga attcatggaa tccagaaatt cctggttggt ttccgttttc ttgatttttc
     3301 cgatgataaa ttccatcgca tcaaccggat tcatttcctg cagcagacgc cgcagaatcc
     3361 agacgcggtt gagattggct ttgggaatca gcagctcttc cttgcgcgtg ccggaccgga
     3421 tgaggtcgaa ggcggggaag atacggcgat cggcgatgcg gcggtcgagg tgcagttcca
     3481 tgttgccggt gcccttgaac tcttcgaaaa tcacttcgtc catgcggctg cccgtgtcga
     3541 tgagcgccgt ggagataatg gtgagcgagc cgccgttttc gatgttgcgc gccgcgccga
     3601 aaaagcgctt cggtttgtga agggcgttgg aatcgacgcc gccggaaagc accttgccgg
     3661 aaggcggaac gaccgtgttg taggcgcggg ccagccttgt aatcgagtcg agcaggatga
     3721 cgacgtcctt tttgtgctcc acgaggcgct tggctttttc aatcaccatt tcagccacct
     3781 ggacgtggcg cgacgcaggc tcgtcaaagg ttgacgagat gacttcgcct ttcacgctgc
     3841 gctgcatgtc ggtgacctct tcgggacgct cgtcaatcag caaaaccatt aaaacgactt
     3901 ccttatgatt ggccgtgatg ctgtgggcga tgtcctgcag cagaacggtt ttgccggcgc
     3961 gcggcggcga gacgatgagc gcgcgctgtc ccttgccgat cggcgcgaac aaatccagcg
     4021 tccgggtgga aaaattttcc ggatcaaatt ccaggttgag tttttcctgg ggataaagag
     4081 gggtcaggtt gtcaaagagg attttatcgc gggccgactc ggggctttca aaattgactt
     4141 catcaacttt cagaagggcg aaatattttt caccttcttt ggggggacgg acttcgccgg
     4201 aaatggtgtc gccggttcgc aagttaaagc ggcggatctg tgacggtgaa atgtagatgt
     4261 cgtccgggct gggcagatag ttgtaatcga ccgcgcgcag gaagccgaag ccgtccggaa
     4321 ggatttccaa aacgccggag ccggagatga cgccgttttg ttccgcctgg gtttgcagaa
     4381 tggcaaaaat caggtcctgg cggcgcatgc cgctggctcc cgggacttcc agctctttgg
     4441 ccagattgtt gagctcgctg atgggcagcc tcttgatgtc ttcgatgt
//
LOCUS       DAOSIM010000171         4466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_17218_length_4466_cov_7.231467, whole genome shotgun sequence.
ACCESSION   DAOSIM010000171 DAOSIM010000000
VERSION     DAOSIM010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17218_length_4466_cov_7.231467"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="PLR47_07760"
     CDS             complement(<1..948)
                     /locus_tag="PLR47_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOG12879.1"
                     /translation="METQTDFLIIGSGIAGLSLAIKVAQLGSVAIVTKKEKSESNTNY
                     AQGGIAAVTDKTDSFEEHISDTLECGAGLCRKDVVEFVVREAPPRVQELIEWGVNFTK
                     SEEPPHLYDLGQEGGHHRRRVLHAKDLTGQEIERALHEKVAALKNVRIYENHIGIDLI
                     LRKDPQGRTVNCLGAYVLDINEDEVRTFRAKYTILSTGGAGKVYLITTNPDIATGDGI
                     AMAYRAGAKIANMEFIQFHPTCLFHPEAKAFLISEAVRGEGGILKLKNGATFMEKYHP
                     MNSLAPRDVVAKAIDNEIKQSGDEYVLLDISHHSREFLLN"
     gene            1060..2085
                     /gene="tsaD"
                     /locus_tag="PLR47_07765"
     CDS             1060..2085
                     /gene="tsaD"
                     /locus_tag="PLR47_07765"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOG12880.1"
                     /translation="MFVLGIESSCDETAAAVVRDGKLLSGVIASQIKDHSAYGGVVPE
                     LASRKHIEAIVPVVEQSLKEAGASFRDLEGIAVTRGPGLIGSLLVGLSAAKALAYALG
                     IAFVGVNHLEAHIMASSLGEKKPRFPFAALVVSGGHTNVYSVKNYHRFALLGQTRDDA
                     AGEALDKAAKLLDLGYPGGVVIDRMARDGNPEAYDFPRAMKDSPDFSFSGLKTSLLVM
                     MKKRGKGFAESELPDVVASYQEAVVDVLVEKTLRAARENGLSQIVICGGVAANSRLRE
                     KFSEAATREGMELFFPPMILCTDNAAMVAALGEVMLENGRSDSLDLNAVSRWPLVPRF
                     PEENGKE"
     gene            2093..2965
                     /gene="rsmA"
                     /locus_tag="PLR47_07770"
     CDS             2093..2965
                     /gene="rsmA"
                     /locus_tag="PLR47_07770"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOG12881.1"
                     /translation="MTTPGEILRRYGIRPDKRRGQSFLTDSNTVRKIAQAAKAAPEDI
                     VVEIGAGFGVLTRQIAGQAGRLIAVEIDARLVEILQKELAEFERVEIFQNDILKFDFS
                     YISKQYKSRIKVVGNVPYNISSPVVFHLLAHRSAISEFTLMLQKEVVERLISPPGHKS
                     YGVPSVLLQMHADVERLFDVSATCFYPRPKVESSVIHGTFRDQPLVELTDEAFFRRIV
                     KAAFAQRRKMLFNNLKHAQILENMSGAEIQSALDESGIDGKRRGETLSLKEFALLSNI
                     LKSKAQPSGEFVNG"
     gene            3022..4347
                     /gene="miaB"
                     /locus_tag="PLR47_07775"
     CDS             3022..4347
                     /gene="miaB"
                     /locus_tag="PLR47_07775"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOG12882.1"
                     /translation="MKNKYLYIETFGCQMNVHDSGQMAVLLRDLGYEPTEDSAKADCI
                     LINTCSIREKAEQKAMSELGRLMKLKEKNPNLIVGFAGCLAQHLGARVHGRVKNVDLV
                     FGTHNIHRLPEMMARVQKERKPVTEVGFSSSLHSLGIFAPPPKGAVGAFVSIMQGCDN
                     YCAYCVVPFLRGPEMSREPKDIVGEIEKLAGRGIREVTLLGQNVNSYGKKTGGRGDFV
                     FLLKKINDIEGIERIRFTTSHPRDLSEELISCFGSLRKLCRHIHLPVQSGSSRILKRM
                     NRGYTAEEYLEKVARLRRVCPEISITSDIIVGFPGETQKDYQETIDMMEKIRFDSVFS
                     FKYSERKGTAAQAIEGKVPETEKKLRLREVQALQDRHTQEKNTSLEGSVQEVLVEGQS
                     RNSRQDMTGRTSSWKIVNFEGRSELIGRTVPVRISRGYLHSLRGKQITP"
BASE COUNT         1009 a         1115 c         1331 g         1011 t
ORIGIN      
        1 gttcaggaga aattcgcggc tgtggtgcga gatgtccaga aggacgtatt catccccgga
       61 ttgcttgatt tcattgtcga tggctttggc caccacgtcg cgcggcgcaa ggctgttcat
      121 ggggtggtat ttttccatga aggtggcgcc gttcttcagc ttcagaatgc cgccttcgcc
      181 gcggacggcc tcgctgatta aaaacgcctt ggcttccgga tggaacaggc aggtggggtg
      241 gaactggatg aactccatgt tggcgatttt tgcgccggcg cggtaggcca tggcaatgcc
      301 gtcgcccgtg gcgatgtccg gattggtggt gatcagatag accttgcccg cgcctcccgt
      361 cgatagaatc gtatatttgg cgcggaaggt gcggacttcg tcctcgttga tgtcgagcac
      421 gtaggcgccc agacagttga cggtccggcc ctgcggatct ttccggagga tcaggtcgat
      481 gccgatgtgg ttttcgtaaa tccggacgtt tttcagcgcc gcgacttttt cgtgaagcgc
      541 ccgctcgatt tcctggccgg tgaggtcttt ggcgtgcagc acgcggcgcc ggtgatggcc
      601 cccctcctgg cccagatcgt aaaggtgggg aggctcttcc gatttcgtga aattgacgcc
      661 ccattcgatc agttcctgaa cgcgcggcgg cgcctcccgc acgacaaatt caacgacgtc
      721 cttccggcag aggcccgcgc cgcactccag cgtgtcgctg atgtgctctt caaaactgtc
      781 ggtcttgtcc gtgaccgcag cgatgccgcc ctgggcgtag ttggtgttgg attccgattt
      841 ttcctttttg gtgacgatgg cgaccgaacc gagctgcgcc actttgatgg ccagactcaa
      901 acccgcgatt ccgcttccga tgatgagaaa atcagtttgt gtttccatgt gagaaaagcc
      961 cgctcattcc cgccgctaaa agtttgttta caaatgccgg gggcttttat ataagccttt
     1021 ttcgcggttt gtaaagaaaa ttatgaaaaa ggagtttcct tgtttgtttt aggcattgag
     1081 tcatcctgcg acgaaacggc ggccgcggtg gtccgggacg gaaagttgct ctccggcgtg
     1141 attgcgtcgc agatcaagga tcacagcgca tacgggggcg tggtgccgga gctcgcctcg
     1201 cgaaagcata tcgaagcgat cgttcccgtg gtggagcagt ctttgaagga agcgggcgct
     1261 tccttccggg atctcgaggg gattgccgtc acccgcggcc cgggcctgat cggctcgctc
     1321 ctggtcgggc tctccgccgc caaggctctg gcctatgcgc tgggcattgc gtttgtcgga
     1381 gtcaaccatt tggaggcgca catcatggcg tcttcgctgg gggagaaaaa accgcggttt
     1441 ccgtttgccg ctctggtggt ttccggcggt cacaccaatg tgtattcggt gaaaaattac
     1501 caccggttcg cgctgctggg ccagacgcgg gatgatgcgg cgggagaggc gctggacaag
     1561 gcggccaaac tgctcgacct gggctacccc ggcggcgtcg tgatcgaccg gatggccaga
     1621 gacggaaatc cggaggccta cgattttccc agagccatga aagattcgcc ggatttcagc
     1681 ttcagcggac tcaaaacatc ccttttggtc atgatgaaga agcgggggaa gggctttgcg
     1741 gagagtgagc ttccggatgt cgtcgcaagc taccaggaag cggttgtgga cgtcctggtg
     1801 gagaaaacgc tcagggcagc ccgggagaat ggcctttctc aaattgtcat ctgcggcggc
     1861 gttgccgcca acagccgttt acgagagaag ttttccgaag cggccaccag ggaaggcatg
     1921 gagctgtttt tcccgccgat gattttgtgc acggacaacg cggcgatggt cgcggccctg
     1981 ggggaggtca tgctggaaaa cggaagaagc gattcactgg acctcaatgc cgtatcgcga
     2041 tggcctctgg ttccccggtt tccggaggaa aacgggaagg aatgagcatg ctatgaccac
     2101 acccggggaa attcttcgcc ggtacgggat tcgccccgat aaaagacgcg gccagtcctt
     2161 cctgacggat tcgaatacgg tgcgtaaaat cgcccaggcc gcaaaggcag cgccggaaga
     2221 cattgtcgtg gaaatcggcg ccggcttcgg cgttctgaca aggcagatcg ccggacaggc
     2281 cggcaggctc atcgccgtgg aaatcgatgc ccgcctggtg gaaattctcc aaaaggagct
     2341 tgcggaattt gagcgagtgg agattttcca aaatgatatt ttaaaattcg atttctctta
     2401 tatatcaaag cagtataaat caaggattaa agtagtcggt aatgttccgt acaatatttc
     2461 cagccccgtt gtttttcatc tgctggcgca ccggtcggcg atttccgaat ttacgctgat
     2521 gctgcaaaag gaagtggttg aaagacttat ttccccgccg gggcataaat catacggcgt
     2581 gccttccgtt ttgcttcaga tgcatgcgga cgtggaaagg ctttttgatg tatcggcaac
     2641 ctgcttctat ccgaggccga aagttgagtc ttccgtcatt cacggaacat ttcgggatca
     2701 accgctcgtt gaactcacgg acgaagcttt tttcagaaga atagtgaagg cggcttttgc
     2761 ccagcgccga aagatgcttt tcaacaattt aaaacatgcg caaattctgg agaacatgtc
     2821 cggggcggaa attcaatccg ctcttgatga atccggcatc gatgggaaaa gaagaggaga
     2881 aacgttgtct ttaaaagagt ttgccctgct gagcaatatt ttgaaatcaa aggcccagcc
     2941 atccggcgaa ttcgttaatg gataggacgg atattttctc ctgtgatata atgctcacgt
     3001 caaataaatg acaggatggt catgaaaaac aagtatttat atattgagac cttcggttgt
     3061 cagatgaacg ttcacgactc cgggcagatg gccgttttgc tccgtgatct cggttatgag
     3121 ccgactgaag attccgcaaa ggcggactgc atcctcatca acacctgttc gattcgcgag
     3181 aaggccgaac agaaggcgat gagcgaactg ggccgcctga tgaagctcaa ggaaaagaat
     3241 ccgaatctga tcgtcggttt cgccggctgt ctggcccagc atctgggcgc gcgggttcac
     3301 ggccgcgtga agaatgtcga tctggttttc ggcacgcaca atattcacag gctgccggaa
     3361 atgatggcgc gcgtccaaaa ggagcgcaag ccggtcacgg aagtcggctt ttcatcgtcg
     3421 cttcattcgc tgggcatctt tgcgccgccc ccaaaaggcg cggtcggggc cttcgtgtcc
     3481 attatgcagg ggtgcgataa ttactgcgcc tattgcgtcg ttccgttttt gcggggaccg
     3541 gaaatgagcc gggaaccaaa ggacattgtc ggggaaattg aaaaactggc cgggcgcggc
     3601 attcgggaag tgacgctttt ggggcagaat gtcaattcct acggaaaaaa aaccggcggc
     3661 cgaggcgatt ttgtgttttt attgaaaaag atcaatgata ttgaagggat tgaaagaatt
     3721 cgcttcacca cgtcgcatcc cagagacctt tcggaagaac tgatctcctg cttcggaagc
     3781 ctgcgcaagc tttgccgtca cattcatttg ccggtgcagt ccggctccag ccggatttta
     3841 aagcggatga accgggggta caccgccgaa gaatatctgg aaaaagtggc gcgcctgcga
     3901 cgggtgtgtc ccgaaatcag catcacgtcc gacatcattg tcggctttcc cggagaaacc
     3961 caaaaagatt atcaagaaac cattgacatg atggaaaaaa tcaggtttga ttcagttttt
     4021 tcgttcaagt attctgaacg caagggaacg gccgcacagg cgattgaggg caaagtgccg
     4081 gaaacggaaa aaaaattgcg gctcagggaa gtccaggcgc ttcaggacag gcacacgcag
     4141 gaaaaaaata cgtcgctgga aggcagcgtc caggaagtgc tggtggaagg gcaaagccgg
     4201 aactccaggc aggatatgac ggggcgaaca agctcctgga aaattgtcaa ttttgaagga
     4261 cgttcggagc tgatcggccg gacggtcccg gtgagaatat cgcggggata tctgcactca
     4321 ctgaggggaa aacagatcac gccataagga ggttgcgaat gctgctggag atgaaggtgt
     4381 ccgggttgac catcgatccg atcaccaaca cgccgatcgt catcctgaaa gacctgcagg
     4441 aaagcaaagc gattccgatc tggatc
//
LOCUS       DAOSIM010000172         4457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_17254_length_4457_cov_7.389141, whole genome shotgun sequence.
ACCESSION   DAOSIM010000172 DAOSIM010000000
VERSION     DAOSIM010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17254_length_4457_cov_7.389141"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..1323
                     /locus_tag="PLR47_07780"
     CDS             184..1323
                     /locus_tag="PLR47_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG12883.1"
                     /translation="MAFEITSEQEMIRLMARDFARKELEPMAAAWERQGIFPMDAVKK
                     MGELGLLGMMVPPELGGSGAGAVAYSLALQEIAYACASSAVTMSVANLSTEPLLKFGS
                     PEQKEKWLVGLAEGSLLGAFALTEPGAGSDPGSMTTTAVLRKDKYIINGTKVFITHGQ
                     YADVINLIARTGQERGAKGLSAFIIEKGMPGFTIGTKEDKLGLRASNTVELVFEDCEV
                     PAKNLLGNPGEGFKVAMLALDSGRIGIASQAVGIARAALDEALSYTQQRRQFGKTINS
                     FQAIQFMIADAATRIEAASWLTLAAADRKDRGLKFTKEASMAKLYASETANKAAYMAL
                     QVHGGYGYTKEYKVERLYRDARATTIYEGTSEVQRIVIAREVIKP"
     gene            complement(1345..2124)
                     /gene="otsB"
                     /locus_tag="PLR47_07785"
     CDS             complement(1345..2124)
                     /gene="otsB"
                     /locus_tag="PLR47_07785"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HOG12884.1"
                     /translation="MSQDSSAQYLFEKNSFPVLEHFLDRSTLFAFDLDGTLAPIVSHP
                     DAAGIPDAVQEELSVLAGKAPVAVITGRSRPDALRHLMFAPRYLVGNHGAEGLSGWKA
                     REKAFVRATRDWQRQLEALIPAEDREGIVMENKGSTISVHYRHAENRKKAHAMIRRAA
                     GRLVPPPRLVGGKCVENLIPEDAPDKGAALKLLMRQAGCSKAFFAGDDETDEDVFRLN
                     HKRILAVRVGRKNDSSARFFLREQSEIARLLREINRILGRS"
     assembly_gap    2266..2307
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2308..4356)
                     /locus_tag="PLR47_07790"
     CDS             complement(<2308..4356)
                     /locus_tag="PLR47_07790"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HOG12885.1"
                     /translation="MSLQSIKSSRSLRLTLRFVVPLVAAMALLAYAVVPLVDKLTLRW
                     SVRDMDIRSKLVTSTLQEPLGELLEQGNKKKIQSLLARATQDERLFALGYCDVQGNLL
                     FKTRAFPDGLGCSIPDPSLRKTPSLLELPQGLVHVAVNPFLMEGMPAGSLVLLHDMSF
                     FRQRSEDTRKYLLVLFAVLSLVTSVITVLIAHLSWRGWMTGVRALLRGEGLIRPFARS
                     SSSELQPLVGDLRALLRTMDAEKRMADDLTLSWNPDSLRSLLFQQLAGEQILVVSNRE
                     PYIHMKGREGIEIQRPASGLVTAVEPVLRACSGIWIAHGSGTADREVVDQKDYVRVPP
                     EHPEYTLRRIWLTEEEEKGYYYGLANEGLWPLCHIAHVRPIFRTGDWNRYVAINQRFA
                     DAVAREAERDDPVVLVQDYHFALLPGMLRQSLPRATIITFWHIPWPNPESFGICPWRE
                     EILRGMLGSTIVGFHTPFHCKNFLETVDRYLETRVNYASSTISHGGNLTLVEAYPISI
                     QWPSPWQETQPPVARCREDIRRELNLGKDILLGLGVDRLDYTKGILERLRAVECLLET
                     HPDMAGRFCFIQIAAPSRSVLEEYQAFDARVRALAAQINERFSRDGYQPVHLRVEHHE
                     PRDINRYYRAVDVCLVTSLHDGMNLVAKEFVAARDDERGVLVLSRFTGAAHELYEALI
                     V"
BASE COUNT          976 a         1333 c         1293 g          813 t
ORIGIN      
        1 aaggggaaga gttcgtctat gtcttaaagg gaaagctttc catttcggtc ggcaggaaca
       61 ccagccttct gggaccgggc cagtgcattc acttcaattc ggcgctgcat cacgcgctga
      121 gcaatccttc ccccgacaaa acggaacttc tcgtcgttct gtatattccc tgaaggaaaa
      181 acgatggcct ttgaaatcac aagcgaacag gaaatgatcc ggctcatggc cagggatttt
      241 gccagaaaag agctggaacc gatggcggcg gcatgggagc gtcagggcat tttcccgatg
      301 gacgcagtca aaaaaatggg ggagctgggc cttttgggca tgatggtgcc gcctgaactc
      361 ggcggctccg gcgccggcgc ggtagcctac tccctggcgc tgcaggaaat cgcctatgcg
      421 tgcgcgtcaa gcgcggtcac catgtcggtc gccaatctct ccacggagcc gcttctcaaa
      481 ttcggcagcc cggagcagaa agaaaaatgg ctggtgggac ttgccgaagg atcgcttctc
      541 ggcgcgtttg ctctgacgga gccgggcgcc ggctccgacc cgggatcgat gacaaccacc
      601 gccgtccttc ggaaggacaa atacatcatc aacggcacca aagtctttat cacgcacggc
      661 cagtatgccg atgtcatcaa cctgatcgcc cggacgggcc aggaaagagg cgccaaaggc
      721 ctctcggcgt tcatcatcga aaaaggcatg cccgggttta ccatcggaac caaagaggac
      781 aaactggggc tgagggcgtc caataccgta gaactggtgt tcgaggactg cgaggttccc
      841 gcgaaaaacc tgctgggcaa tcccggcgag ggattcaagg tggccatgct cgccctggac
      901 agcggcagaa tcggcattgc ctcccaggcc gtcggcatcg ccagagcggc gctggatgaa
      961 gcgctttcct atacgcagca aaggcgtcag ttcgggaaaa ccatcaattc gttccaggcc
     1021 attcaattca tgatcgcgga tgccgcaacc cgaattgaag cggcatcctg gctgacgctg
     1081 gccgcggccg accgcaagga tcgcggactg aagttcacca aggaagcttc catggccaaa
     1141 ctgtacgcct cggaaacggc caacaaggcc gcctacatgg cgctgcaggt ccacggcgga
     1201 tacggctaca ccaaagagta caaagtcgaa cgcctgtacc gggacgcccg cgccacgacc
     1261 atctatgaag gcacatcgga agttcagcgc atcgtcatcg ccagagaagt catcaaacca
     1321 tgatccttcg caagaaagcc ggactcagga tcgcccgagt atccggttga tctcgcgcaa
     1381 aagacgggcg atttcagact gctcccgaag aaagaagcgg gcgctgctgt cattcttcct
     1441 gcccacacga acggcgagaa tgcgcttatg gttcagccgg aaaacatctt catcggtctc
     1501 gtcatctccg gcaaaaaacg cctttgaaca ccccgcctgc cgcatgagca gcttcagagc
     1561 agcgcctttg tccggagcgt cttccggaat cagattttca acgcatttgc ccccgacaag
     1621 cctgggcggc ggaaccagcc ggccggcggc ccggcgaatc atggcatgtg cttttttccg
     1681 gttttcggcg tgccggtagt gaacggaaat cgttgaaccc ttgttttcca tcacgatgcc
     1741 ttcgcggtct tccgccggaa tgagcgcttc aagctgacgc tgccagtccc gcgttgcgcg
     1801 gacaaaagcc ttctcccgcg ccttccatcc cgaaagccct tccgcgccgt ggttcccgac
     1861 cagataacgg ggcgcaaaca tcagatgcct gagggcgtcc gggcgggaac gcccggtaat
     1921 gaccgcaacg ggtgcctttc cggcaagaac ggacaattcc tcctggaccg catcgggtat
     1981 tccggcggca tccgggtgcg atacaatggg cgccagcgtg ccgtcgagat caaaggcaaa
     2041 gagcgttgac cgatccagga aatgctcgag aacggggaaa gagttctttt cgaagagata
     2101 ttgagcggac gaatcctggc tcatagcgcg acaccgatcc gttccgccag cttttcgcgc
     2161 cggcggatgc gcgccgcgtc cagaagcatt cgggccgccc accggtaaac attgaaatct
     2221 ctcaccatgg accgcatgct gcgcatccgc gcctgttgtt cgaatnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnngac gatcagggct tcatacagct cgtgggccgc
     2341 tccggtaaaa cggctgagca caaggactcc ccgttcgtcg tcgcgggccg ctacgaattc
     2401 cttggccacc aggttcatgc cgtcatgcag gctcgtcacc aggcagacgt ccacggcgcg
     2461 gtagtagcgg ttgatgtcgc gcggttcgtg atgctccacc ctcagatgaa ccggctgata
     2521 gccgtcgcgg gaaaatcgtt cgttgatctg agcggccaaa gcccgtacac gggcatcaaa
     2581 ggcctggtat tcttcaagaa cggatcgcga cggagcggcg atctggatga agcaaaaccg
     2641 gccggccatg tccgggtgcg tctccagcag acattcgacg gccctcaaac gctcgagaat
     2701 tcccttggtg tagtccagcc ggtcaacgcc gagccccagc aaaatatcct tcccgaggtt
     2761 cagctcacgt cgtatgtctt ccctgcagcg ggcgaccggc ggctgcgttt cctgccacgg
     2821 cgacggccat tggatggaaa tcggataggc ttcaaccagc gtgagattgc cgccgtggga
     2881 aatcgtggaa gacgcgtaat tcacccgcgt ttcgaggtag cggtccacgg tttccaggaa
     2941 attcttgcaa tggaaaggcg tgtgaaaacc gacaatcgtg cttcccagca tgcctcgcag
     3001 gatttcttcg cgccaggggc aaatgccgaa ggattccgga ttcggccagg gaatgtgcca
     3061 gaaggtgata atggttgccc tgggaagaga ctggcggagc atgcccggca gcagagcaaa
     3121 atggtagtcc tgaaccagaa cgaccgggtc atcccgttcg gcttcccggg ccaccgcgtc
     3181 ggcaaaacgc tgattgatcg ccacgtaccg gttccagtct ccggtccgga aaatgggacg
     3241 cacatgcgca atgtggcaca gcggccagag gccctcgttg gccaaaccgt agtagtatcc
     3301 cttctcctct tcttccgtaa gccagatgcg ccgaagcgta tattcaggat gctccggcgg
     3361 cacgcggaca taatcctttt ggtccacgac ctcccggtcg gccgtgccgc tgccatgggc
     3421 gatccatatt ccggagcagg cgcgcaggac cggctccacc gccgtcacca gtccgctggc
     3481 cgggcgctga atctcaatcc cttccctgcc tttcatgtga atgtacggct cccggttgga
     3541 aaccacaaga atctgctccc ccgccagttg ctggaaaagc aaactgcgga gcgaatccgg
     3601 attccaggaa agagtgagat catcggccat gcgcttttcc gcatccatcg tgcgcaggag
     3661 ggcgcgcaga tcacccacca gcggctgaag ttcggagctg gaagaccttg caaaaggcct
     3721 gatcaggcct tcgccgcgca gcagggcgcg gacgcccgtc atccagccgc gccagctcag
     3781 atgggcgatc aggacggtga tcacggaggt caccagggaa agaacggcaa acaggaccag
     3841 aagatacttg cgcgtgtcct cgctgcgctg ccggaaaaag ctcatgtcgt gcagcaacac
     3901 cagggagccg gccggcattc cctccatgag aaagggattg acggcaacat gcaccagccc
     3961 ctgcggcaat tccagcagcg atggggtttt ccggagagaa ggatcaggaa tggaacagcc
     4021 gaggccgtcg ggaaaagccc gggttttgaa gagcagattt ccctgaacgt cgcagtaccc
     4081 cagcgcaaaa aggcgctcgt cctgcgtggc gcgggccagc agggactgaa tctttttttt
     4141 gttgccctgt tccagaagtt cgcccagagg ctcctggagt gtgctggtca ccagttttga
     4201 ccgaatgtcc atgtcccgca ccgaccagcg cagcgtcagc ttgtccacca ggggcaccac
     4261 cgcgtaagcc aggagcgcca tggccgcgac gaggggaacg acaaagcgca gcgtaagtct
     4321 cagcgaccgg gaagatttga tgctttgaag gctcatcctt ccctcccttc taaaacgcgc
     4381 tgtcccagcg cctacttaag cgaatggcgg agttgatcag gcccaccatg ctgtaggtct
     4441 gaacaaaatt cccccag
//
LOCUS       DAOSIM010000173         4448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_17308_length_4448_cov_6.806738, whole genome shotgun sequence.
ACCESSION   DAOSIM010000173 DAOSIM010000000
VERSION     DAOSIM010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17308_length_4448_cov_6.806738"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(363..1553)
                     /locus_tag="PLR47_07795"
     CDS             complement(363..1553)
                     /locus_tag="PLR47_07795"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG12886.1"
                     /translation="MKALIDEAGSQLIRRRFADASELIPAVYASLVDYLAVKELLRAG
                     PFDAAACANASLDDIDPEKVIKFVSLARRAREFPLPEGTQPKEVLAHLSLLNKERPTN
                     AAMLLFGKTPQRFLISSEIKCAHFHGLEVSKPIPFYQVYKGTVFDLVDQAVDFVLSKI
                     NLAVGTRELSVQAPVAYEMPPEVIREAIVNAVAHRDYTSNGSVQVILFSDRLEVWNPG
                     TLPPSLTLEKLRHPHGSVPGNPLLAEPLYLTKYIERMGTGTGDMIARCRKAGLPEPEF
                     ALTDGFVATVRRRPERAFEAVGGKKEAPGRRQEGTRLALSGNQVEILNQCSDACAIAD
                     LMQLVGRSDRTKFRNQVLNPLIQEGLLMMTVPDKPTSSLQKYRATERGRALLAKLKKK
                     GSGK"
     gene            complement(1608..1898)
                     /locus_tag="PLR47_07800"
     CDS             complement(1608..1898)
                     /locus_tag="PLR47_07800"
                     /inference="COORDINATES: protein motif:HMM:NF024668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HOG12887.1"
                     /translation="MSALRMKIFISSVQKEFAKERKALRDYVQGDPLLRRFFDVFLFE
                     DLPARDQRADDVYLEEVHNSDIYLALFGNQYGAPDKKGFSPTHREFNEATRL"
     gene            2342..>2833
                     /locus_tag="PLR47_07805"
     CDS             2342..>2833
                     /locus_tag="PLR47_07805"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOG12888.1"
                     /translation="MNKTGRNNSLIDVEGIMVGHFTSRKYTCGTTAILCPEGATAGVD
                     VRGSAPGTRETDLLQPMNLVEKVQAVVLTGGSVYGLATADGVVRWLEKKGWGFPLQDG
                     YVAPIVPAAALFDLGRGKQFVPPIGPKWGAAACSAAGKGVFPLGSVGAGTGAMAGGIK
                     GGLG"
     assembly_gap    2834..2843
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2844..3357
                     /locus_tag="PLR47_07810"
     CDS             <2844..3357
                     /locus_tag="PLR47_07810"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOG12889.1"
                     /translation="EALPSGIIVAAIVAVNSLGSVIDPAMGRFWEIRQEAESEFGDLG
                     KRAVKLPPPPVASAGQNTTIGVIATNAVLTKAQAQKIAQMAHDGFARAIRPSHTMFDG
                     DTIFCIGTNKRKLPATRGFFASPAAEILTEMGRSAADCMTRAITRGILEATSLSGIRA
                     FRDLDARGDS"
     gene            3396..>4448
                     /locus_tag="PLR47_07815"
     CDS             3396..>4448
                     /locus_tag="PLR47_07815"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HOG12890.1"
                     /translation="MTTQKKFFLFFGIGAAAFVVLLASAWLFVSWKLSQPDAFKEPLT
                     RMIGRELNRPVHYDEGSGSLSLRKGLAFTFTGFVIKEKDGVADFVHIRSARLRLDILP
                     LLRNQFSLRDVLLDRPVLQLKRDSNGELNIADLLAKPRKETALKINNINIDKGSVTFE
                     DQAAGNEVITTRLDDLDCAVDSPLFGSRFQYQISATVIEDRNKATLSLKGTFEPSPSD
                     KPVWASTLNASIRLKGSDLRHYAPYLNRRAAIDRLGGLLDADTNFSGTLKNFSSKGSV
                     AVKNSLFDCARAFSNPLTPRTVRIDYALSRNAGSLSLDVARLAIDRFEASGRLNIEDL
                     DKEDFFLTAIAKTSAFS"
BASE COUNT         1048 a         1178 c         1156 g         1056 t
ORIGIN      
        1 agaaatcgcc agcagtatgc cgcttcctct tttcatgcgc ggcatcatac acgactttgg
       61 cctttgtgac taccggatag attgagacac gtcaagccgt tctcaagggt agaaattaga
      121 aaaactggag ctgaaatcct gcgctttatc agatgatatt agtcccagcc ttttgcatta
      181 agtatataag tcacattcaa cgggtcttgc gcttctttta cacgacggac tgaaactcgg
      241 cagtgggaat atttttccgc ttcgcttcat catgcgtcag tgtttcaatg gttttgttgt
      301 ttgttttctt tgctgccatc ttctctacct ttcagatgcc gtgaaaaggc aaggttcgat
      361 tgtcactttc cactcccttt ctttttcagt tttgccagaa gggctctccc tctttccgtc
      421 gcgcggtatt tctgaagaga gctggtcggc ttatccggga cagtcatcat caaaaggcct
      481 tcctggatca gcggatttaa aacctggtta cggaacttgg tgcgatccga acggcccacc
      541 aactgcatca gatcggcaat cgcgcatgca tctgagcatt gattcaggat ttcaacctgg
      601 ttcccactta gtgccaacct ggtgccttcc tggcgcctgc cgggcgcctc tttcttgccg
      661 ccgacggctt caaaggcccg ctccggcctt cggcgaacgg ttgcgacaaa gccgtccgtc
      721 agggcaaatt cgggttcagg caatccggct ttacgacaac gcgcaatcat atcacccgtt
      781 cccgtgccca tacgttcgat atatttggtc aaatataaag gctccgccag aagtggatta
      841 ccgggcacgg agccatgcgg gtgccttaat ttttcgagtg taagcgaagg cggcagtgtg
      901 ccgggattcc agacttcgag ccgatctgaa aaaagtatca cctgaacgct gccgttgctg
      961 gtgtaatcgc ggtgcgcaac agcattgaca atggcctccc gaatgacttc gggcggcatt
     1021 tcataagcga caggggcctg gacgctcaat tcacgcgtgc cgacagccaa gttgattttg
     1081 gagagcacaa aatcgactgc ctgatcgact aaatcaaaaa cagtgccctt ataaacctga
     1141 taaaaaggaa tgggtttgct gacttccagg ccatgaaaat gcgcgcattt aatctccgat
     1201 gaaatcagaa atcgttgtgg tgtcttcccg aataaaagca ttgcggcatt cgtaggacgt
     1261 tccttattca gtaagctcag atgcgccaaa acctcctttg gttgtgtacc ttcaggcaga
     1321 ggaaattccc tggcgcgacg ggccagactg acaaatttga tgaccttttc cggatcgata
     1381 tcgtccaatg acgcatttgc gcatgcagcg gcgtcaaagg gtccggcgcg gagaagttcc
     1441 tttacggcca gataatccac cagactggca taaactgcgg ggatcagctc cgatgcgtcg
     1501 gcgaaacgac ggcggatgag ctgtgaaccc gcttcgtcaa taagcgcttt catttggggt
     1561 tgccggtttt catccgtcgc cccattaaca aaaatgagac gttcctttca cagccgggtt
     1621 gcttcgttga attcacgatg cgtcggggaa aaaccttttt tatcgggcgc gccatattga
     1681 ttgccgaaca aagccagata aatatcgctg ttgtgaactt cttcaagata gacatcatcc
     1741 gcgcgctgat ctctggcggg taaatcttca aagagaaata cgtcgaagaa gcggcgcaaa
     1801 agcggatcgc cctgaacata atcccgaagc gctttgcgct ctttggcaaa ttccttttga
     1861 acactgctga taaatatttt cattcgcaag gcagacatgc ttaacctttg gccatcgtaa
     1921 actttccaat catgttgtca aactcttttt cgaccttcgc tttaaagtcg gattcaatct
     1981 gatagacttc ggtaaattcg gcaaaggccc aacggccata ctggccatta ttattaacgc
     2041 cgggaatcca gtaggtgaat ttaggatcgg cttttcaaaa aagatgtttt ccatgttcat
     2101 ttccttatcc cgaggcaaga tcggcagcag atacagtaac taaatgtcgg tctagatttt
     2161 actaatggcg gaacggagta taggaagaac atcacgagat gtcaatgaaa taatagctga
     2221 aatgcctttc gacctgtggt aaatgaaacg caatcgacaa gcggattttg atcataagtt
     2281 gatcagcgat gatcgcgatc atttgaagac gtcgcacaac ctttttcgca aggagatgcc
     2341 gatgaataaa acaggccgga ataattctct gatcgatgtt gaaggaatca tggtcggtca
     2401 tttcaccagc cggaaataca cctgcggcac aaccgcgatt ctctgccccg agggagcgac
     2461 ggccggcgtg gatgttcgcg gctccgcacc gggaacgcgc gaaacagacc tgttgcagcc
     2521 catgaatctg gtggaaaagg tgcaggcggt cgtgttgacc ggcggatccg tatatggcct
     2581 ggcaacggca gacggcgtcg tgcgctggct cgaaaagaaa ggctggggat ttccgctgca
     2641 ggatggctat gtggcgccta ttgtgccggc ggccgcgctt ttcgatctgg gccggggaaa
     2701 acagtttgtt ccaccgattg gtccaaaatg gggagcggcg gcatgcagcg ccgcgggcaa
     2761 aggcgttttc cccctcgggt ccgtcggcgc gggaacgggc gccatggccg gagggatcaa
     2821 aggcggcctg ggannnnnnn nnncgaagcc ctgccctcgg gaatcatcgt cgcggcgatt
     2881 gttgccgtga actctctggg ttccgtgatc gatcccgcga tggggcgttt ctgggaaatc
     2941 cgtcaggaag cagaatcgga attcggagac ctcggaaagc gagcggtgaa gctgccaccg
     3001 ccacccgtgg cttccgcggg acagaacacg accatcggag tgatcgcgac caatgcggtt
     3061 ttgaccaaag cgcaggcgca aaaaattgcc cagatggctc acgacggctt tgcccgcgcc
     3121 atacgcccgt cacacacgat gtttgacgga gacacgatct tttgcattgg aaccaacaag
     3181 cgaaaattgc ccgcgacccg gggatttttc gcatctccgg cggcggaaat tctgaccgaa
     3241 atggggcgca gcgcagcgga ctgcatgacc cgagccatca cccggggaat cctggaagcc
     3301 accagcctta gcgggatcag ggccttccgg gatctggacg cgcgcggcga ttcttaacgc
     3361 tccgcgatcc aggatcatct taaaaaggct tctgtttgac gactcaaaag aaattctttt
     3421 tattttttgg aatcggcgct gccgcgttcg tcgttcttct tgcatccgcc tggctcttcg
     3481 tttcgtggaa actatcccaa cccgatgcct tcaaggaacc tctgacaaga atgatcggca
     3541 gggaactgaa ccgccccgtc cattacgatg agggaagcgg ctctctttcc cttcgcaaag
     3601 gactggcatt tacctttacc ggcttcgtta tcaaagaaaa ggacggcgtt gccgacttcg
     3661 ttcacatccg ttctgcccgt ctgcgcctgg atattcttcc acttcttaga aatcaattct
     3721 cactcagaga cgttcttctg gatcggcccg ttcttcaact gaagcgggac agcaacgggg
     3781 aattgaacat cgccgatctg ctggcaaagc cccggaagga aacggcgctg aaaatcaaca
     3841 acatcaacat tgacaaagga tcggtaacct ttgaggatca ggccgcggga aatgaagtga
     3901 tcaccacccg tctggatgat ctcgactgcg ccgtcgattc ccctttgttc ggaagtcggt
     3961 ttcaatatca aataagtgcg actgtaatcg aagatcggaa caaagcgacc ctttccctga
     4021 aaggcacctt tgaaccctca ccgtccgata aacctgtttg ggcaagcacc ctgaacgcgt
     4081 ctatccggct caaaggatcg gaccttcggc attacgcgcc ttacctgaat cgcagggcgg
     4141 ccattgatcg gctgggcggc ctgctggatg cggacacaaa cttttccggg accctgaaga
     4201 atttttcatc aaagggcagc gtcgcggtga aaaactctct gttcgactgc gccagagctt
     4261 tttccaatcc gctcactccg cggacggtcc gcatcgacta cgctttgagc cgcaacgccg
     4321 gaagtctttc gctggacgtg gcgcgcctgg cgatcgatcg ctttgaagca agcggccgtc
     4381 tgaacatcga ggacttggac aaggaagatt tcttcctgac cgcaattgcc aaaacgtccg
     4441 ccttttcc
//
LOCUS       DAOSIM010000174         4446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_17318_length_4446_cov_8.826008, whole genome shotgun sequence.
ACCESSION   DAOSIM010000174 DAOSIM010000000
VERSION     DAOSIM010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17318_length_4446_cov_8.826008"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PLR47_07820"
     CDS             <1..462
                     /locus_tag="PLR47_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOG12891.1"
                     /translation="NMSIESVLKELQPHLGKEVFVSGWKQVTQAQINQFADSTGDHQW
                     IHVDAEKAAAGPFGRTIAHGFLTLSHIPFFSYQAPVKIEGAKMSINYGLDKVRFLNPV
                     PSGSNIRDRIVLSGVEEKPGNRLLIRQTHTIEMEGQDKPACVAEALAMVFF"
     gene            complement(560..1534)
                     /locus_tag="PLR47_07825"
     CDS             complement(560..1534)
                     /locus_tag="PLR47_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG12892.1"
                     /translation="MADEIRKGLFRISVPLPNSPLKDLNSYVIRGEDRNLIIDTGFNR
                     TVCYEAMQAGLAELGIDLNQTDFMLTHMHADHTGLVTRLATKTSRIFFGRIDARVFDG
                     DHGWQSLIDFAALNGFPVDELQKALASHPGFKYSPQQKPVFTLLDDGDVIECGGYRLR
                     CVATPGHTQGHICLYEEDQRIFFSGDHVLFDITPHIESWAYETNSLSDYLASLDRVYA
                     LPVELVLPGHRNFQGDLKDRIRELKHHHRERADEVLEVLGGRAMNAYEIAAGMTWDID
                     CETWEDFPIAQKWFATGEAIAHLRFLEGEGRIHRHTDQAVVAFSAVRP"
     gene            complement(1599..3470)
                     /locus_tag="PLR47_07830"
     CDS             complement(1599..3470)
                     /locus_tag="PLR47_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOG12893.1"
                     /translation="MKHVMLVNAVHKEQMRMATVDEKGKLIDFNIQMAVREPITGNIY
                     KGKVLKVERGLQAAFVDYGGVKDGFLPLRDVSHEYLTEPENGSSGGRAVLKTGQEIIV
                     QVVREIIGNKGALLTSYISLPGRYLVLLPNKLCGGISRKIESEEDRRKIKTLMDQIKV
                     PEGMGFIVRTAGINRTKQEIQRDYQFLMRLWKEITKKAEDASEPSLLYQETDFGVRSL
                     RDYLTQEMDEIWVDEVETFRKMRAYLKAVAPRHLRILKQYKDKTPIFERNSLEEQIRV
                     IYQERVELKSGGYIIINPTEAMITIDVNSGRGSNKRNIEETAFRTNVDACEEIARQLR
                     LRDLGGLIVIDFIDMIDKKHIAEVEKAFKKALSMDRARIQLSRISKFGLMELSRQKKQ
                     STIQEISYTTCPYCAGSGLRPSLEYASLGAFRKIESEAVKGTYSELRVSLPHEIALYI
                     LNNKRTELLKLESTFGVCLIIEGKPDMAWDNFEIQQSMKEQALEVSAIQGVPLLNLAE
                     KTEPDAEGGEKPEAEAGEAEEPPRKKGRRRSRHKKRKSPEALKGSVATEDALPDAQPS
                     PGGMAPPVSSSQFSRFPVKPLKIVGALPEEFFPVDLSGDLFTPEMDQEDTGPEQEDN"
     gene            complement(3542..4180)
                     /locus_tag="PLR47_07835"
     CDS             complement(3542..4180)
                     /locus_tag="PLR47_07835"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOG12894.1"
                     /translation="MEAQRKARARTVLSINSQNPQMRLIRKATDVLRDGGIIIYPTDT
                     VYGLGCDLSNKRGIEKIYEIKRRNKKRPLSFVCSDLKHISQYALVTDYAYKTMKRFLP
                     GPYTFILEASRLVPRIILPKRPTTGIRVPDNQICLALIRELGQPIISTSVQTLDGEDL
                     GDPVIINEHFGRVVDLIIDGGIIVPEPSSVVSLVDDRIEVLRTGKGDVSAFI"
     gene            complement(4236..>4446)
                     /locus_tag="PLR47_07840"
     CDS             complement(4236..>4446)
                     /locus_tag="PLR47_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOG12895.1"
                     /translation="IKEMHLVDFKVRTLEGSEGTAAGVRVLLDSTDGSELWSTTGVSE
                     NIIEASWQALIDSIEYKLSKDRKKR"
BASE COUNT          868 a         1313 c         1239 g         1026 t
ORIGIN      
        1 aatatgtcga tcgaatcggt actaaaagaa ttgcagccgc acctcgggaa ggaagttttt
       61 gtttccggct ggaaacaggt gacgcaggcg cagatcaacc agttcgccga cagcaccggc
      121 gaccaccagt ggattcatgt ggacgcggaa aaggccgccg ccggaccgtt cggccggacc
      181 atcgcgcacg gattcctgac gctttcgcac atcccttttt tcagctacca ggcgcccgtg
      241 aagatcgaag gcgcgaagat gtccatcaac tacggtctgg acaaggtgcg ttttctcaat
      301 cccgttcctt cgggatcgaa catccgcgac cggatcgtgc tgtccggcgt ggaagaaaag
      361 ccgggcaacc gtcttttgat ccgccagacg cataccattg aaatggaagg gcaggacaaa
      421 ccggcgtgcg tcgcggaagc gctggccatg gtttttttct gacaggtcat cgagcgggga
      481 acggttgcag accgttcccc gctttggttt gatttgaaat gaagcggcag accatcgccg
      541 gcatcctgaa cgggacgaat cagggacgga cggccgaaaa ggctacaacc gcctgatcgg
      601 tatggcggtg aatccggcct tctccctcca ggaagcgcag atgcgcgatg gcttccccgg
      661 tggcaaacca cttctgggca atcggaaaat cctcccaggt ttcgcagtcg atgtcccagg
      721 tcatccccgc ggcaatctca taggcattca tcgcccggcc gcccagaacc tccagcactt
      781 catccgcccg ttcgcggtgg tgatgcttca gctcgcggat cctgtccttc agatccccct
      841 gaaaattccg gtgtccgggc aggaccagtt ccaccggcag ggcatacacc cggtccaggc
      901 tggccaggta gtccgaaagc gaattggtct cataggccca cgactcgatg tgcggcgtaa
      961 tgtcgaacag gacgtggtcg ccggaaaaga aaattctctg atcctcctca tacaggcaga
     1021 tgtgcccctg cgtgtggccc ggcgtggcga cgcatcgcag ccggtagcct ccgcattcga
     1081 ttacgtcgcc gtcgtccagc agggtaaaga caggcttttg ctgcggactg tatttgaatc
     1141 ccggatgact ggcgagcgcc ttctgaagct cgtcgacggg aaaaccgttc agggcggcga
     1201 agtcaatcag ggactgccag ccgtgatcgc cgtcaaaaac gcgcgcgtcg atcctgccga
     1261 agaatattcg gctggttttc gtcgccagcc tcgtcaccag gccggtgtgg tcggcatgca
     1321 tgtgggtcag catgaaatcg gtctgattca gatcgatccc cagttcggcc aggcctgcct
     1381 gcatcgcttc gtagcagacg gttcggttga aacccgtgtc gatgatcaga ttgcggtctt
     1441 cgccccggat cacgtaggag ttcaggtcct tgagcggact gttcggcaac ggcacggaga
     1501 tccggaaaag cccctttcgg atttcatccg ccatgcgctg tcctccctga cgcagagacg
     1561 gagcggtccg ggccttcggg aagtcttcga agggtcattc agttatcctc ctgctccggg
     1621 cccgtatctt cctgatccat ttccggcgtg aaaagatcgc cgcttaagtc caccggaaag
     1681 aattcttccg gcagcgcgcc gacaatcttc agcggcttga cgggaaaacg cgaaaactgc
     1741 gaggaggaaa ccggaggcgc catcccgccg ggggaaggct gcgcgtcggg caaagcgtct
     1801 tccgtcgcaa cggagccttt taatgcctcc ggcgattttc ttttcttgtg gcgggaacgg
     1861 cgccgcccct ttttcctggg gggctcttcg gcttcgccgg cttccgcttc cggcttttca
     1921 ccgccctccg cgtccggctc cgtcttttcc gcaaggttca gcaggggcac tccctgaatg
     1981 gcgctgactt ccagggcctg ctccttcatg gactgctgaa tttcgaaatt gtcccacgcc
     2041 atatcgggtt ttccttcgat aatcaggcag acgccgaagg ttgactccag cttcaaaagc
     2101 tcggtccgtt tgttgttcag gatgtacagg gcgatttcat gcggcaggct gaccctcagc
     2161 tccgaatagg tgcccttgac cgcctccgat tcgattttgc gaaacgcccc gagagaggcg
     2221 tactccagag aggggcgcag accgctgccg gcgcagtacg gacaggtcgt gtagctgatt
     2281 tcctgaatcg tggactgttt tttctgccgg gacagctcca tcaggccgaa cttggagatg
     2341 cgcgaaagct gaattctggc gcgatccatg ctcagcgcct tcttgaaggc cttttccact
     2401 tcggcaatgt gcttcttgtc gatcatatcg atgaaatcga tgacgatcag accgcccagg
     2461 tcgcgcagcc tgagctggcg cgcgatttct tcacaggcat ccacattcgt ccggaaagcg
     2521 gtttcttcga tgtttcgctt gttcgacccg cgtccggaat tgacgtcgat ggtgatcatg
     2581 gcttccgtgg gattgataat gatgtagccg ccggacttga gctccacgcg ttcctggtaa
     2641 atcacccgga tctgctcttc cagcgagttg cgctcaaaaa tcggagtctt gtccttgtac
     2701 tgcttcagga ttcgcaaatg gcggggggcg accgctttca ggtacgcgcg catcttgcgg
     2761 aaggtttcca cctcgtccac ccagatttcg tccatttcct gcgtcaggta gtcgcgcagc
     2821 gaccgcacgc cgaaatccgt ctcctggtag agcagagacg gttcggaggc gtcttcggcc
     2881 ttcttggtga tctccttcca caggcgcatc aggaactggt agtcgcgctg aatttcctgc
     2941 ttggttcggt tgatcccggc cgtgcggacg ataaaaccca tcccctcggg aaccttgatc
     3001 tggtccatca gcgtcttgat cttccggcgg tcttcctcac tttcgatttt ccgggagatg
     3061 ccgccgcaga gcttgttggg aagcagcacg agataccgtc cgggcaggga gatgtacgac
     3121 gtcagcaggg cgcccttgtt gccgatgatt tcccggacca cctgcacgat gatttcctgg
     3181 cccgtcttca gaacggccct gccgccggag gaaccgtttt ccggttcggt caggtattcg
     3241 tggctgacgt cgcgcagggg aagaaaaccg tccttgacgc cgccgtaatc cacaaaagcc
     3301 gcctgcaggc cgcgttcgac cttcagcacc ttgcctttgt aaatgttccc ggtgatcggt
     3361 tcccgaacgg ccatttgaat gttaaaatca atcagcttgc ctttttcgtc aaccgtggcc
     3421 atgcgcattt gctccttgtg cacggcgtta acgagcatta catgtttcat aaggatcctt
     3481 ttgggcggcc tttcccgggc cgcgcggctg acggacatga catcccgccg cttcgtgtat
     3541 tttatataaa ggcgctcacg tcgccttttc cagttcgaag aacttcaatc cggtcgtcca
     3601 ccaggctgac gacgctcgac ggctcgggaa cgatgatccc tccgtcaata atcagatcca
     3661 ccacgcgccc gaagtgttcg ttgatgatca cgggatcgcc gaggtcttcc ccgtccaggg
     3721 tctgaacgct ggtgctgata atcggctgcc cgagctcccg gatcaaagca agacaaatct
     3781 ggttgtccgg aacgcgaatg cccgtcgtcg gccttttcgg caaaatgatc cgcggaacca
     3841 gacgggatgc ttccagaata aacgtgtagg gtcctggcaa aaaccgcttc atggttttat
     3901 aagcataatc cgtgaccagc gcgtactgac tgatgtgctt cagatcggaa cagacaaaac
     3961 ttaaagggcg ttttttgttt cttcttttaa tctcgtatat tttctcaatg cctcgcttgt
     4021 tcgataaatc acagcccagg ccgtacactg tatccgtcgg gtagatgata atgccgccgt
     4081 ccctcaacac atccgtcgcc tttcgaatga ggcgcatctg gggattctgg ctgtttatgg
     4141 acaacaccgt tcgagccctc gctttacgtt gggcttccat atcagcaatt gaaagattta
     4201 gcaatggctg caaacggcca aaaaatcagc ggtcgtcacc tctttttccg gtctttgctg
     4261 agcttgtact cgatgctgtc gatgagcgcc tgccagctcg cctcgatgat gttttcggaa
     4321 acgcccgtcg tgctccagag ctcggagccg tccgtcgaat ccagcaaaac gcggacgccc
     4381 gcggccgtgc cttcggagcc ttccagcgtg cgcaccttga agtccaccag gtgcatttcc
     4441 ttgatt
//
LOCUS       DAOSIM010000175         4380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_17650_length_4380_cov_10.623815, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000175 DAOSIM010000000
VERSION     DAOSIM010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17650_length_4380_cov_10.623815"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2001)
                     /locus_tag="PLR47_07845"
     CDS             complement(<1..2001)
                     /locus_tag="PLR47_07845"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOG12896.1"
                     /translation="MNQKMKTILQITPLKITLFVILIALALFLLDFKFLRLVELKTLD
                     LRIASRGDLKPGGETVIAVIDEKSLSELGRWPWPRTTIAQLVRKLKSEGAKAVGFDIV
                     FSEPDINSNLKTIDALSAEMKKRGVSQSGVTDLLRRKRAAADTDAILASAMRETGNVT
                     LGYFFHFARKGSDKELAHVTAQRIAQNARRIENSRYPMVNSTSGKPSDVFLPHAFAPE
                     ANIPALSVAGRNSGYFNALPDSDGSNRWSPLVIAFQNNYYSSLAVSLVQSYLDFPNLS
                     LNLEPFGAKSVVIDDIVVPTDESGQLLINYMGPPQTFPHYSISDILAGRLPKDTFRGK
                     IVLVGATAVGIYDLRVTPFSATFPGVEIHANVIDNILHRNFLIHSSVTRFIDVCSIVL
                     FGLILGLLIPRLRPITGMIAAFLMIAAFALINFFIFFSFNTWLNLVYPLITMATIYLG
                     ITIYHYFKEEREKKKIRGAFQYYLTSSVINEMLKDPDKLKLGGDKKDLTVLFSDIRGF
                     TTISEKMTPEELVALLNEYLTTMTNQVFHYDGLLDKYMGDAIMAVFGAPLDQPDHALR
                     ACRTSLAMMKELHKLQEKWKAEGRPAFDIGVGLNSGDMVVGNMGSEMRFDYTVMGDMV
                     NLGSRLEGINKEYGTNIIISEFTYDKVKDAMCCRELDGVRVKG"
     gene            2241..2537
                     /locus_tag="PLR47_07850"
     CDS             2241..2537
                     /locus_tag="PLR47_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12897.1"
                     /translation="MSKVKPGDVLNCEVCGLSVIVDEECGCAFADLICCEELMVNKGP
                     RPPKKAAAAPKKKTAAKQAKPAAKKPAAPKPAAKKPAVKKAAPKPAAKKKPAKK"
     gene            complement(2567..4102)
                     /locus_tag="PLR47_07855"
     CDS             complement(2567..4102)
                     /locus_tag="PLR47_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HOG12898.1"
                     /translation="MTAPLCIAKSGEKEIVLLPAMANRHGLITGATGTGKTVTLQKMA
                     ESFASIGAPVFLADVKGDLSGIAAAGVASEKLMKRLEGIGIKEFHPRANTAVLWDVFG
                     EKGHPVRATVSDMGPMLLARLLNLNDTQSGVLTLVFKIADDNGLLLLDLKDLRAMIQF
                     VGDNAKQFTTEYGNISAASIGAIQRGILTLDEQGGDKFFGEPMLNIADLMQTDTEGRG
                     VVNVLAADKLYNSPKLYSTFLLWMLAELFEQLPEVGDVEKPKLVFFFDEAHLLFNDAP
                     AALLEKIEQVVRLIRSKGVGVYFVTQNPLDIPESVLGQLGNRVQHALRAFTPRDQKAV
                     KTAADTMRPNPAFNTADVITELGVGEALVSFLDEKGRPAPVERSFVIAPGSRLGVLTD
                     AERQAAVQRSTVYGFYEKTLDRESAYEKLRTSVAEAKTPAQPQAQTGKTEAGQEEPQA
                     KGGGILGGAVGDLLFGRSGPRGGKTDGLVQASAKSLARTIGSTVGRQIVRGILGSLLG
                     GKK"
BASE COUNT          933 a         1277 c         1269 g          901 t
ORIGIN      
        1 gcctttgacg cggacgccgt cgagttcgcg gcagcacatg gcgtctttca ctttgtcata
       61 ggtgaactcg ctgatgatga tattggtgcc gtattccttg ttgatccctt ccagccggga
      121 gcccaggttg accatgtccc ccatgaccgt gtagtcgaag cgcatctccg atcccatgtt
      181 gcccacgacc atgtcgccgg aattcagacc gacgccgatg tcaaacgcgg ggcgcccttc
      241 cgccttccac ttttcctgaa gcttgtgcag ctccttcatc atggccaggg aggtccggca
      301 ggcccgcagg gcgtggtccg gctgatccag cggcgcgccg aagaccgcca tgatggcgtc
      361 gcccatgtat ttgtccaaaa gcccgtcgta atggaacacc tggttcgtca tcgtcgtcag
      421 gtattcattg agcagggcga ccagctcctc cggcgtcatt ttttcggaaa tggtggtgaa
      481 gccccgaatg tccgagaaca ggacggtcag gtctttcttg tcgccgccca gcttgagctt
      541 gtccgggtcc ttcagcattt cgttgatgac cgaagaggtc agatagtact ggaaagcgcc
      601 ccggattttt ttcttttccc gctcttcctt gaagtagtgg taaatcgtga tgcccagata
      661 aatcgtcgcc atcgtgatca gcggatagac aaggttcagc caggtattga agctgaagaa
      721 aatgaagaaa ttgatcaggg caaaggcggc gatcatcagg aatgcggcga tcattccggt
      781 gatcgggcgc aggcggggaa tcaaaagccc gagaatcagc ccgaaaagaa caatcgagca
      841 gacatcgatg aaccgggtca cggaggagtg gatcaggaaa ttccggtgca gaatgttgtc
      901 gatgacgttg gcgtgaattt caacgccggg aaacgtggcg gagaaaggcg tcacgcgcag
      961 atcgtaaatg ccgacggccg tcgcgccgac cagcacgatc ttgccgcgga aagtgtcctt
     1021 cggaagccgt ccggccagaa tgtcggagat ggagtaatgg ggaaacgtct gtggcggccc
     1081 catgtaattg atcagcagct gccccgattc atccgtcgga accacgatat cgtcgatgac
     1141 cacgcttttg gcgccgaagg gctcgaggtt cagcgacaga ttgggaaaat caagatagga
     1201 ctgaaccagg gaaaccgcca gggaagaata gtaattgttc tgaaaagcga tcaccagcgg
     1261 cgaccagcgg ttcgacccgt cgctgtccgg aagggcgttg aaatagccgc tgttgcgtcc
     1321 cgcgacggac agggcgggaa tattggcttc cggcgcgaag gcgtgcggca gaaaaacgtc
     1381 gctgggtttg cccgacgtgg aattgaccat cggatagcgg gagttttcta tgcgccgggc
     1441 gttttgtgcg atgcgctgcg ccgtgacgtg cgccagctcc ttgtcggacc ccttgcgcgc
     1501 aaaatgaaag aaatacccca gcgtgacatt gccggtttct ctcatggcgg aagccagaat
     1561 cgcatccgtg tccgcggcgg ctctttttct gcgcagcaga tcagtgacgc cggattggga
     1621 aacgcctcgt tttttcattt cggcggaaag cgcgtcgatg gttttcagat tggagttgat
     1681 gtccggttcg gaaaatacga tatcaaagcc gaccgccttg gcgccttcgc ttttcagctt
     1741 ccgcaccagt tgcgcgatcg ttgtccgggg ccagggccac cggcccagtt cggaaaggct
     1801 tttttcatcg atgacggcaa tgacggtttc accgccgggc ttcagatcgc cgcgcgacgc
     1861 gatgcgcaaa tccagcgttt tgagctccac caggcgcaga aatttaaagt ccagcaaaaa
     1921 aagcgccagc gcgatcaaaa tgacaaaaag cgtgatcttc agcggcgtaa tctgcaaaat
     1981 ggtcttcatt ttctgattca tgccgtcccc tcccccgggc gaaaatcata ccagacggat
     2041 gcgtaaagtc aagctgtcct ttgtttcagg gcgctttgcc tttttcccgt ttggcggccc
     2101 gcagacgccg ggcgcgcccc ccaaaaagcg gccccgcgaa ttcaacgctt gaaagaaaaa
     2161 cggttttgca ctacaaagaa cggcatcgga tacgtcagga tcggaaggaa gaactcacaa
     2221 acaacgagga ggaattttaa atgtcaaagg taaagcccgg tgatgttttg aattgcgaag
     2281 tatgcggact ctcggttatt gtggatgagg aatgcgggtg cgcttttgcc gacctgatct
     2341 gctgtgaaga gctcatggtc aataaaggcc cccggccgcc gaaaaaagcg gccgccgcgc
     2401 caaagaaaaa aacggcggcg aagcaagcga agcctgcggc caaaaaaccg gcggccccca
     2461 aaccggccgc caagaaaccc gcggtgaaaa aggccgcccc caaaccggcc gccaaaaaga
     2521 aaccggcaaa aaaataatcg ggccggcccg ccttggccgg gcgcgtctat ttttttccgc
     2581 ccagcagaga ccccaggatc ccgcggacaa tctgccgccc gacggttgac ccgatcgttc
     2641 gggcaaggct cttggccgac gcctgaacca ggccgtccgt ttttccgccg cgcggcccgg
     2701 agcgtccgaa cagcaaatcc cccacggcgc cgcccaggat gccgccgccc ttggcctgag
     2761 gctcctcttg gccggcttcg gttttgccgg tttgagcctg cggctgcgcc ggagtcttcg
     2821 cttccgcgac ggaggtccgc agcttttcgt aggcggattc gcggtccagc gttttttcat
     2881 agaaaccgta aaccgttgac cgttgaacgg ccgcctgccg ctcggcatcg gtgagcacgc
     2941 cgagccggga gccgggcgcg atcacaaaag acctctccac cggcgccgga cggccttttt
     3001 cgtccagaaa tgaaaccagc gcctctccga cgcccagctc cgtaatcaca tccgccgtgt
     3061 taaaggcggg attgggccgc atggtgtccg ccgccgtttt caccgccttc tgatcgcgcg
     3121 gcgtgaaggc gcgcaaggcg tgctggacgc ggttccccaa ctgccccagg acgctttccg
     3181 gaatatcgag aggattctgc gtcacgaaat acactcccac ccccttggag cggatcagcc
     3241 gcaccacctg ctcgatcttt tccagaagag cggccggcgc gtcgttgaac agcaggtgcg
     3301 cctcgtcaaa aaagaacacc agtttgggct tttcaacatc ccccacttcc ggcaactgtt
     3361 caaagagttc ggccagcatc cacaaaagaa acgtggaata cagcttgggg ctgttgtaga
     3421 gcttgtcggc cgccagcacg ttgaccacgc cccgcccctc agtgtccgtc tgcatcaggt
     3481 cggcaatgtt cagcatcggt tctccgaaga atttgtcgcc gccctgctcg tccagcgtca
     3541 gaatcccccg ctgaatcgct ccgatggaag ccgcggaaat gttgccgtat tccgtggtga
     3601 actgtttggc gttgtcgccc acgaactgaa tcatggcccg cagatccttc agatccagca
     3661 gcagcaggcc gttgtcgtcg gcaatcttga aaaccagcgt cagcacgccc gactgggtat
     3721 cgttgagatt gagcagcctc gccagcagca tcgggcccat gtcggagacg gtggcgcgca
     3781 ccgggtgccc cttttccccg aacacgtccc ataacacggc cgtgttggcg cgcgggtgga
     3841 attccttgat gccgatccct tccaggcgct tcatcaattt ctcggaagcg acgcccgcag
     3901 cagcgatgcc ggagaggtcg cccttgacgt cggccagaaa aaccggcgct ccgatggacg
     3961 cgaaggattc ggccattttt tgcagcgtga cggttttccc cgttccggtt gcgccggtaa
     4021 tgagcccgtg gcggttggcc atggcgggca gcagaacgat ttccttttct ccggactttg
     4081 cgatgcaaag cggtgcggtc atgacggcct cctgatgtga atttttgccg atggatcttt
     4141 caacttcttt gagcgcgact atagaaagaa tccggccgtc tgtcaatcat cattcgaaaa
     4201 gcgcgatcct tcaaaaaaac acaaaaaatc acttttccgg cccccttcac ggcgcttgac
     4261 cgcccggttt ttcggtggta gagcaggctc aaatttacgt gggggccatg tgaacagcca
     4321 caggcgggaa cctttctgga gcggcgtggt ccgttccatg ggcattgtgt tcggcgacat
//
LOCUS       DAOSIM010000176         4351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_17819_length_4351_cov_12.318669, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000176 DAOSIM010000000
VERSION     DAOSIM010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17819_length_4351_cov_12.318669"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PLR47_07860"
     CDS             <1..1009
                     /locus_tag="PLR47_07860"
                     /inference="COORDINATES: protein motif:HMM:NF017808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG12899.1"
                     /translation="QDTFMLYLPEGRYRLFAVTDYNHNGKYEKNEASGVYGSGSQVLE
                     IAVREGELISGVEIRTAAADEHPVQLPWEWRLKKDEPIILQATGNGDVLKIYHEYFSL
                     ENAQVGYWNPSSFMKMFGAHIYFAQAYDPRKIPLLFVHGTEGSPQNWIYFDMRLDRSK
                     YQPWFFYYPSGIRLNLASALLYEELRELHEKFGFRKMALAAHSVGGLTARSYLNRYGS
                     EDSNAFLRLFASFATPWSGFGLADASQILPHKSIPVWLDLGTQSDFISTTMDAKLPSA
                     IQYYLFYGKTDKLSGDTALDSRAVAGAAGVYGFDCTHDGILSDRRVFFQFNAILEKEM
                     F"
     gene            1011..1814
                     /locus_tag="PLR47_07865"
     CDS             1011..1814
                     /locus_tag="PLR47_07865"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOG12900.1"
                     /translation="MKIVRFISKRQDTLYGVYDPQRPGTARPIEGDPWNAENGGREEP
                     IERLLAPVAPVNILALGINYRKHGEETALSVPAEPILFLKATSSVTGPDHPILLPAAG
                     PDQVDYEAELAVVIEKTAKNIAPAEAPDYIFGYTCANDVSARDWQFDRQKGQWARGKS
                     FDTFCPLGPWIVTKDEIENPDDLSIRAVLNGKTVQESRTSEMIFDVRTIVSRLSRSMT
                     LHPGTVILTGTPEGVGFARRPPLFLKPGDEIRVEIEKIGTLSNRVIAES"
     gene            1811..2158
                     /locus_tag="PLR47_07870"
     CDS             1811..2158
                     /locus_tag="PLR47_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12901.1"
                     /translation="MKIIRTRWVSRRRLAAINLFGIVFAPKGEILAPRTLNHEAIHTA
                     QMKEMLFIFFYLWYFLEWLFRLRRKTSAYRRISFEREAYYHEHDKDYLKNRKPYHWIR
                     YLSQTTENRPRSE"
     gene            2159..2713
                     /locus_tag="PLR47_07875"
     CDS             2159..2713
                     /locus_tag="PLR47_07875"
                     /inference="COORDINATES: protein motif:HMM:NF022586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HOG12902.1"
                     /translation="MQTTWKTGSEKIYTRAIDISTREYDAERIVVEGFLRDDRYQDTH
                     TITGETFPRGTLHHMSIRLLVNCSTLVIEDLDMELISVPREVCHETMGFLAPVKGMTI
                     ARGFTARIKKLVGGPKGCAHVVELLLAMAPAVLQGVAAARSRRPSSVDAGQSKIILKY
                     LINTCHAWREDGPFVKALKASGIC"
     gene            2714..3934
                     /locus_tag="PLR47_07880"
     CDS             2714..3934
                     /locus_tag="PLR47_07880"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG12903.1"
                     /translation="MTATAKEDSDRDTETTPWRSTLLVMCVAQLFSIVGFAFVLPFIP
                     FYIREIGVTDEKLVPVWSGILLAASSLTMTIFAPIWGFLSDRFGRKVMVERAMFAGAF
                     ITMAMGMIDNVYQLLVLRLISGAFTGTISASISLVSSVLPGTRMGLGLGLMQVAVFLG
                     MSVGPWIGGMIADIIGYRLTFIAGGVILLVGGLLVLIGAKEKFIRPSAESLKQGGSMK
                     TLFAMPGFISLLVVFFIFHFSINIAIPILPLFIEEVGHLKTRVASTTGLLLAVSGATA
                     CISAAAIGYLSDRKGHKKVLVVNLFITSLMWIAHALSRNISQLLVVRILFGFAAGGNL
                     PTMNALVGKLTPRESYGKAYGLMASMTSLGMTLGPLFGGIMASYAGLRWPFVIVSFLL
                     SFIMIPIILRLKVQ"
     gene            complement(4053..>4351)
                     /locus_tag="PLR47_07885"
     CDS             complement(4053..>4351)
                     /locus_tag="PLR47_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12904.1"
                     /translation="TLVRVLTRRKLPTNITATWDFSPEIFSNIESNLQIVKPTSSALK
                     TDQSLKGIKGIVLSDGSVIEGKILKMSTDTVIIQTQDGKVSSYSFEKEVRCFVK"
BASE COUNT          967 a         1201 c         1156 g         1027 t
ORIGIN      
        1 tcaggatact ttcatgctgt acctgcccga ggggcgctac cggctttttg ccgtaacgga
       61 ctacaatcac aacggtaagt acgaaaagaa tgaagcctcc ggcgtgtacg gatcgggaag
      121 ccaggttttg gaaattgccg tccgggaggg cgaactgatt tccggcgtgg agatccggac
      181 cgccgcagcc gatgaacatc cggtgcaact gccctgggaa tggcgcctga aaaaggatga
      241 gcctatcatc ctacaggcaa ccggcaacgg agatgttctg aaaatttacc atgaatattt
      301 ttctttggaa aacgcccagg tcggctactg gaatccgtcg tccttcatga aaatgttcgg
      361 cgcgcatatc tattttgcgc aggcctatga tccccggaaa attccgctgc tcttcgtgca
      421 cggcacggag ggcagtccgc aaaactggat ctactttgat atgcgtctgg accgcagcaa
      481 ataccagccc tggttctttt attatccgtc gggaattcgc ctgaatcttg cctcggccct
      541 gctctatgaa gaattgcgtg agcttcacga aaaattcggt ttccggaaaa tggccctcgc
      601 cgcgcacagc gtcggcggcc tgaccgcaag atcttacctg aaccgttatg ggtcggaaga
      661 tagcaatgcc ttcctccggc tgtttgcatc ctttgccaca ccctggagcg gattcggcct
      721 ggccgacgcg tcccaaattc tgccgcataa aagcattccg gtctggctgg atttgggaac
      781 gcaaagcgat tttatttcca ccacgatgga cgccaagctg ccttccgcaa tccaatatta
      841 tcttttttac ggaaagacgg ataaactatc gggagatacg gcgctggaca gccgggcggt
      901 ggccggcgcg gccggcgtgt atggattcga ctgcacgcac gacggcatcc tgagcgaccg
      961 cagggtattt ttccaattca atgccattct ggaaaaggag atgttttgag atgaaaattg
     1021 tccgatttat ttccaaaaga caagacaccc tatacggcgt ttacgaccca caacggcctg
     1081 gtacggccag acccattgaa ggagatccct ggaacgcgga aaacggcggc cgcgaagaac
     1141 cgattgaacg gctgctggcg cccgtcgcgc ccgtcaacat tctcgcgctg ggcatcaatt
     1201 accgaaagca cggcgaggaa accgcccttt ccgttcctgc agagccgatt ctgtttctca
     1261 aagccaccag cagcgtcacc gggcccgacc accccatcct gctgcccgcc gccgggcccg
     1321 atcaggtgga ctacgaagcg gaactggccg tggtcatcga gaagacggca aaaaacatcg
     1381 cgcctgcgga agcgccggac tacattttcg gctacacctg cgccaacgac gtcagcgcgc
     1441 gcgactggca gtttgaccgg cagaaaggcc agtgggcccg gggaaaaagc tttgacacct
     1501 tctgtccgct ggggccgtgg atcgtcacga aggatgaaat cgaaaacccc gatgatctgt
     1561 caatccgcgc cgtgctgaac ggaaaaacgg ttcaggaatc ccgcacgtcg gaaatgatct
     1621 tcgacgtccg gacgatcgtc agccgcctga gccgttccat gaccctgcat ccgggaaccg
     1681 tgattctgac cggcacgccg gaaggcgtgg gatttgcgcg ccgtccgccg ctctttctca
     1741 aacccggcga tgagatccgc gttgaaattg aaaaaatcgg aacgttgagc aatcgggtga
     1801 tcgcagaatc atgaaaatca ttcgcacacg ctgggtttcg cggcgccggc tcgcggccat
     1861 caatctattt ggcatcgttt ttgcgccaaa gggagaaatc ctcgcgccgc gaacgctcaa
     1921 ccatgaagcg atccacacgg cgcaaatgaa ggaaatgctc tttatttttt tctatctgtg
     1981 gtactttctg gaatggcttt tccggcttcg aagaaaaacc agcgcctatc gaaggatcag
     2041 tttcgagcgg gaagcctatt atcatgagca cgacaaagat tatttgaaaa atcgaaagcc
     2101 ttaccactgg atccgatatt tgtcacaaac gaccgaaaac cggccaagga gcgaataaat
     2161 gcaaaccaca tggaaaaccg gcagtgaaaa aatttacacg cgcgccatcg atatctcgac
     2221 ccgggaatat gacgcggaaa ggattgtcgt cgagggcttt ctccgggacg accgctacca
     2281 ggacacgcac accatcaccg gcgaaacgtt tccccgcggc acgcttcatc acatgagcat
     2341 tcggctgctg gtcaactgct ccaccctggt gatcgaagat ctggatatgg agctgatttc
     2401 cgttccgcgg gaagtgtgcc acgaaacgat ggggtttctg gccccggtca aaggcatgac
     2461 catcgcccgg ggattcaccg cccgcattaa aaaattggtg ggcgggccca aaggctgcgc
     2521 gcacgttgtg gagcttctgc tcgccatggc cccggcggtc ctgcagggcg tcgccgccgc
     2581 ccggtcgcgc cgtccttcca gcgtggatgc cgggcagtca aaaatcatcc ttaaatacct
     2641 gatcaacacc tgccacgcct ggcgggaaga cggcccgttc gtgaaagcgt tgaaagcgtc
     2701 aggaatttgt tgaatgaccg caacggcaaa agaagattcc gacagggaca cggaaaccac
     2761 gccatggcgt tcaactctcc ttgtcatgtg cgtcgcgcaa ttgttttcca tcgtggggtt
     2821 tgcttttgtc ctgcctttca tccccttcta tatccgtgaa atcggcgtaa cggatgaaaa
     2881 gctggtcccg gtctggtctg gaatcctttt agcggcgtca agtctcacca tgaccatttt
     2941 tgcccccatc tggggatttc tttcagaccg ctttggccgg aaagtcatgg tggaacgggc
     3001 catgtttgcg ggcgctttca ttaccatggc catgggcatg atcgacaacg tctatcaact
     3061 gctcgtcctt cgtctgatct ccggcgcttt cacgggaacc atttccgcct ccatttcgct
     3121 ggtctcctct gtcctgcccg gcacgagaat ggggctggga ctggggttga tgcaggtcgc
     3181 cgtgtttctg ggaatgtccg tggggccctg gattggcggc atgattgccg acatcatcgg
     3241 ctatcgtctt accttcatcg cggggggcgt catcctgctg gtgggcggtc tgctggtgtt
     3301 gatcggggcg aaggagaaat ttatccgtcc ctccgccgaa tcattgaaac agggcgggag
     3361 catgaagacg ctctttgcca tgccgggctt catctcgttg ctggttgtct ttttcatctt
     3421 ccatttttca attaatatcg ccatccccat tctgccgctt ttcatcgagg aagtcggaca
     3481 cctgaagacg cgcgtcgcct ctacgacagg cctgttgctt gccgtcagcg gcgccaccgc
     3541 ctgcatttcc gccgccgcga tcggttatct gagcgatcgc aagggtcaca agaaagtcct
     3601 cgtcgttaat cttttcatca ccagcctcat gtggattgct catgctctgt cgcgaaacat
     3661 cagccagctg cttgtggtcc ggatcctttt cggatttgca gccggaggga acctgcccac
     3721 catgaatgcc ctggtgggaa aactcacccc gagggaaagc tacggaaagg cgtatggtct
     3781 gatggcttcc atgacgtcgc tcggcatgac gctgggacct ctgttcggcg gaatcatggc
     3841 gtcctacgcg gggctgcgct ggccgttcgt catcgtcagc tttctcctga gcttcattat
     3901 gatccccatc atcctgagat taaaagtaca gtagatgtgc tgcatcacac cgggagcatt
     3961 aggtctggct gatccaagca tgggttgatt attactggca gagactaact ttgcaaactt
     4021 gattatttaa tgttgaacca cttgctctcc tatcatttaa caaaacaacg gacttctttt
     4081 tcaaatgaat atgatgatac ctttccgtcc tgtgtctgaa ttatcacagt gtccgtgctc
     4141 attttgagta ttttcccttc gatgacactt ccatcagaca acacaatgcc ttttattccc
     4201 tttaagctct ggtctgtttt taaagcggat gaagtaggct ttactatttg aagattcgat
     4261 tcaatattag agaagatttc gggtgaaaaa tcccatgtcg ctgttatgtt agtagggagt
     4321 ttccttcttg taaggacgcg gactaatgtt t
//
LOCUS       DAOSIM010000177         4336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_17890_length_4336_cov_8.003737, whole genome shotgun sequence.
ACCESSION   DAOSIM010000177 DAOSIM010000000
VERSION     DAOSIM010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17890_length_4336_cov_8.003737"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..538)
                     /locus_tag="PLR47_07890"
     CDS             complement(68..538)
                     /locus_tag="PLR47_07890"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOG12905.1"
                     /translation="MKKHLRKILVMAIIAFIASACASQDRFIVHHTNGTVLDTKTNLM
                     WAARDNGSDVNWTGAKSYCENYAAGHYKDWRMPTSEELAGLYDARQTREAPCAGNFNI
                     HMTTELIQVTCFAAWSSETRSADASLYNFIYGRPDWYLQSHTYGMRALPVRNNK"
     gene            863..1696
                     /locus_tag="PLR47_07895"
     CDS             863..1696
                     /locus_tag="PLR47_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOG12906.1"
                     /translation="MMSENRPAKFAGASRYVLDDELAKIVNISMALEMPLLLKGEPGT
                     GKTMLAHAIAENLRMPLIVLNVKSSMKLIDALYQYDTLTRLNDSRFSDSGRDVSNIEE
                     YIRMGKIGQAFVSDRKVVLLIDEIDKADTDFQDDMLDILDQMQFDIMEIDRTVKAKSR
                     PVIVITSNAKKDLSDPFLGRCNFHHIAFPDREMMRRIIGVHFPDLNRDLTSACLDTFY
                     RLREVRGVEKKPATRELINWMRALQADPDFNVKSLRSAGIPFLGVLFKKSTDLYLAAH
                     Q"
     gene            1778..>2617
                     /locus_tag="PLR47_07900"
     CDS             1778..>2617
                     /locus_tag="PLR47_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12907.1"
                     /translation="MFVNFFYTLRDKGVPVTPTSFLRLQKALGMGLVASLDDFYSVAR
                     SLLVKSEKYFDIYDQAFSAAFRGMADVDPTDAELTEAVQAMLSEWLRDPGGMARALGL
                     SEEQVKQMTPDELVRHFLRRLKEQTEAHHGGSYWIGSRGTSPTGHSGDHPGGMRVGGQ
                     SRNKSAIKVAMERRYRDYSQSGPITASQMGEALKRLKHLQPAGPRDVVNIDKTIDSTM
                     RNAGEIEIIFDRRMADRLKVKLMIDNGGWSMDPYVEIVQALFHYAQFQFKELEIYYFH
                     NTIY"
     assembly_gap    2618..2627
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2628..2904
                     /locus_tag="PLR47_07905"
     CDS             <2628..2904
                     /locus_tag="PLR47_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12908.1"
                     /translation="RLIIVGDASMAPYELNDAHGAIYLGQNEARPSMDYLKFLSRTFR
                     HSVWLNPQPQDSWFYTWTVKQIAGIFPMFELSLDGLDKAVHRLRSRN"
     gene            2917..3486
                     /locus_tag="PLR47_07910"
     CDS             2917..3486
                     /locus_tag="PLR47_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOG12909.1"
                     /translation="MRKQIALIMLLVFLALPALLFAQTQPPRVGAALPDLKIQKPADS
                     AGLKYLGLSGSGTFAVDQVRAQALIIQIFSLYCPYCQKDAPNMNRFFGLIEGNPRLRG
                     KIKILGIGAGNTQFEVNTFKKKYKVEFALVPDGDFKIHKIIGEVRTPYFIIVKLDGPK
                     KREVVYSRLGVHEDVEAFLAETVRLTGIQ"
     gene            3500..4090
                     /locus_tag="PLR47_07915"
     CDS             3500..4090
                     /locus_tag="PLR47_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOG12910.1"
                     /translation="MKRKIACALLFAALFAAPAFALSDAYKGNVFNTGRLKPVDSVLK
                     VKVGQAAPDFTLRAVSGKKVSLTDYRGKKNVVLSFVPAAWTPVCSDQWPGYNVVQELF
                     EENDAVLLGISVDNIPTLHSWTSQMGQLWFDVLSDFWPHGAAASRYGILRSDGTAERA
                     IIIIDRKGIIRYIDVHDINERPTLESIIGQLEKLRK"
BASE COUNT          976 a         1157 c         1240 g          953 t
ORIGIN      
        1 ccgcctcgtt atcctttgaa tgcaacttgg tataaatatc ctcgcaaaag ttagtgttaa
       61 gaatggccta tttgttattc cggaccggaa gcgcccgcat gccgtaggtg tgcgactgga
      121 gataccagtc gggcctcccg taaatgaaat tgtacaaaga ggcgtcggcg ctgcgcgttt
      181 cggacgacca ggcggcaaag caggtcacct ggatcagctc cgtggtcatg tgaatgttga
      241 aattgccggc gcacggcgcc tcgcgggtct gccgcgcgtc gtacaaaccg gcgagttcct
      301 cagaggtcgg catccgccag tccttgtaat gaccggcggc gtaattctcg cagtaggatt
      361 tcgcgccggt ccagttgacg tcgctgccgt tgtctctggc cgcccacatc aggtttgttt
      421 tggtgtcgag gacggttccg ttcgtgtgat ggacgataaa acggtcctgg ctcgcacagg
      481 ctgacgcgat gaaggcgatg atcgccatca ccagtatttt gcgaagatgc tttttcatgg
      541 gaatctcctt atcgttcggg gaaagtaaaa cgaatcaaat ccgcctttcc tgcatgcgcc
      601 aaaagtatag gaaaagcggg tgaacctgtc aagtgaatct tggctctcaa atcctggcta
      661 tggccgagtt cggcgtaaat gaaaatattc cgcgttaatt tttgcgccaa aacgtgttat
      721 ggctccgcaa gcggccaaac aggcccattg gccgaaggtc gcttgaaggc ggcagcggat
      781 gttgttgacg aaacgcttcc cggcattata ttgaacgggt ggcggccgcg gatcaaaaac
      841 acgataagga gcaacgataa agatgatgtc tgaaaatcgc cctgcaaaat ttgccggggc
      901 atcacgctat gtgctggatg atgagcttgc taaaattgtt aatatttcca tggccttgga
      961 gatgccgctt ctgctcaagg gcgaaccggg gaccggcaaa accatgctgg cgcatgccat
     1021 tgccgaaaac ctgcgcatgc cccttattgt tctgaatgtc aaatccagca tgaagctgat
     1081 tgacgccctg tatcagtacg acacgctgac ccgactcaac gacagccgtt tttccgattc
     1141 cgggcgggat gtgagcaata tcgaggaata tatcaggatg ggcaaaatcg gccaggcgtt
     1201 tgtctccgac aggaaagtcg tgctgctcat cgacgagatc gacaaggccg acacggattt
     1261 tcaggatgac atgctcgaca tccttgacca gatgcagttc gatatcatgg aaatcgaccg
     1321 gacggtgaaa gcgaagagcc ggccggtgat cgtgatcacg tccaacgcca agaaggatct
     1381 gtccgatccg tttctggggc gctgcaattt ccatcacatc gctttccccg accgtgaaat
     1441 gatgcgcagg atcatcggcg tgcattttcc ggatttgaac agggatctga cctcggcctg
     1501 cctggatacg ttttaccgcc tgcgcgaggt gcgcggcgtg gaaaaaaagc cggccacgcg
     1561 cgaactgatc aactggatgc gcgcgctcca ggccgatccg gatttcaatg taaaaagcct
     1621 gcggtcggcc ggcatccctt ttttgggcgt gctgttcaaa aaaagcaccg acctttacct
     1681 ggctgcgcac cagtaaaaaa agtccggcag gcgccgccgc cgggttgccc ttgcgggcgg
     1741 gcttccatgc ccgcgccggg ttttcgggag acgagacatg tttgtcaatt ttttctacac
     1801 gcttcgtgac aagggtgttc ccgtgacgcc cacgtctttc ctgcgtctgc agaaagcgct
     1861 gggaatgggg cttgtcgcct cgctggacga tttttacagc gtcgcgcgca gccttctggt
     1921 caaaagcgaa aaatactttg acatctatga tcaggcgttt tccgccgctt ttcggggcat
     1981 ggccgatgtc gatcccaccg acgcggagct gaccgaggcc gtccaggcga tgctttccga
     2041 gtggctcagg gatccgggcg gcatggcccg ggcgctgggg ctttccgaag aacaggtgaa
     2101 acagatgacg cccgacgaac tcgtgcggca ttttctcagg cgcctcaagg agcagaccga
     2161 agcccatcac ggcggcagct actggatcgg ttcgcgcggc acatcgccca cggggcactc
     2221 cggcgatcat ccgggcggca tgcgggtggg cggccagtcg cgcaacaagt ccgccatcaa
     2281 ggtggcgatg gagcgccgct accgggatta ttcgcagtcc ggccccatca cggcttcgca
     2341 gatgggcgag gcgctcaaaa ggctgaagca ccttcagcct gctggccccc gggacgtcgt
     2401 caacatcgac aaaacgattg attcgacgat gcgcaacgcg ggggaaattg aaatcatttt
     2461 tgaccgccga atggccgacc ggctgaaagt caagctgatg atcgacaacg gcggctggtc
     2521 gatggatccc tacgtggaaa tcgttcaggc gctgttccat tacgcgcagt ttcagttcaa
     2581 ggaactggag atttattact ttcacaacac gatttatnnn nnnnnnngcg gctgattatc
     2641 gtgggggacg cgtccatggc gccctatgaa ctcaatgacg cgcacggagc gatctacctc
     2701 ggccagaatg aagcccggcc ttccatggat tatctgaagt ttctgtcccg gacgtttcgc
     2761 cactcggtct ggctcaatcc gcagccgcag gattcgtggt tttacacatg gacggtgaaa
     2821 cagattgccg ggatttttcc gatgttcgag ctgtcgctgg acgggctgga taaagccgtc
     2881 caccgcctga gatcccgaaa ttgacgagga ggagtcatga gaaaacaaat tgctttgata
     2941 atgttgctgg tgtttttagc cctgccggcc ctgcttttcg cccaaacgca gccgccgcgg
     3001 gtgggcgccg cgctgcccga cctgaaaatc cagaaacccg cggattcggc cggcctgaaa
     3061 tatctgggcc tctccggctc cggaaccttt gccgtggatc aggttcgcgc ccaggcgttg
     3121 atcatccaga tcttcagcct ctactgccct tactgccaga aggacgcgcc caacatgaac
     3181 cgctttttcg gcctgatcga ggggaatccg cgtctcaggg gaaaaatcaa gatcctcggc
     3241 atcggcgcgg gaaatacgca gtttgaagtg aacacgttta aaaagaagta caaggttgaa
     3301 tttgcgctgg ttccggacgg ggattttaaa attcacaaaa tcatcggcga agtccgcact
     3361 ccgtatttca tcatcgtgaa actggacggc cccaaaaaac gggaagtggt ttattccagg
     3421 ctgggcgttc acgaagacgt ggaagcgttt ctggccgaaa cggtccgcct gaccggaatt
     3481 cagtaaacgg gaggcctcca tgaaaagaaa gattgcatgc gcgttgctct ttgccgcctt
     3541 gttcgccgct ccggcgttcg ccctttccga cgcctacaag ggaaatgtgt tcaataccgg
     3601 caggctcaag ccggtggaca gcgttttaaa agtcaaagtc ggccaggccg ccccggactt
     3661 cacgctgcgg gccgtaagcg gcaaaaaggt gtcgctcaca gactaccggg gaaaaaagaa
     3721 tgtcgtgctt tcctttgttc ccgccgcctg gacgccggtt tgctccgacc agtggcccgg
     3781 gtacaacgta gtgcaggagc tgtttgaaga aaacgacgcc gtgctcctgg gcatcagcgt
     3841 tgacaacatt ccgacgcttc attcctggac cagccagatg gggcagctct ggtttgacgt
     3901 gctgtccgat ttctggccgc acggcgcggc ggcttcccgt tacggcattc tgcgttccga
     3961 cggcacggcg gagcgggcga tcatcatcat cgatcgaaaa ggcataatcc gctacattga
     4021 tgttcatgac atcaacgagc gtccgaccct ggaaagcatt atcgggcagt tggaaaaact
     4081 gcggaaataa gttttttaat tttccttgac aggggttttc aattgcggta cgcactatga
     4141 ctgtaagtca taaggccgtt ctgttggtat ttgccaagca ttaacagaga cttgcaaaaa
     4201 ggacgatgaa aggccttcat tctgcattcg aaggagagcc gctcttgagc tcggaagaaa
     4261 gaagaaagaa agaaaaggaa aaccggaaaa attccatttt gaaggccgcc cgcaagctgt
     4321 tttttgaacg ggggtt
//
LOCUS       DAOSIM010000178         4310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_18029_length_4310_cov_8.239483, whole genome shotgun sequence.
ACCESSION   DAOSIM010000178 DAOSIM010000000
VERSION     DAOSIM010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18029_length_4310_cov_8.239483"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /gene="rpsE"
                     /locus_tag="PLR47_07920"
     CDS             complement(<1..176)
                     /gene="rpsE"
                     /locus_tag="PLR47_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416232.1"
                     /note="located at the back of the 30S subunit body where
                     it stabilizes the conformation of the head with respect to
                     the body; contacts S4 and S8; with S4 and S12 plays a role
                     in translational accuracy; mutations in this gene result
                     in spectinomycin resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOG12911.1"
                     /translation="MKEVELLDRVIAINRTAKVVKGGRRFRFSAIVVVGDGQGMVGAG
                     LGKAHEVPEAIRKG"
     gene            complement(231..596)
                     /gene="rplR"
                     /locus_tag="PLR47_07925"
     CDS             complement(231..596)
                     /gene="rplR"
                     /locus_tag="PLR47_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOG12912.1"
                     /translation="MASKKTLKVREKREKRVRGKLTGTAERPRLSVFRSDKHIYAQVV
                     NDAQGKTIATASTLSRELAGKIRGIKKVEVAREVGKLIAARLQTLGVEKVVFDRGRFL
                     YHGRVKALADGARESGLKF"
     gene            complement(633..1175)
                     /gene="rplF"
                     /locus_tag="PLR47_07930"
     CDS             complement(633..1175)
                     /gene="rplF"
                     /locus_tag="PLR47_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOG12913.1"
                     /translation="MSRIGKQPITFPKNVKVSQSGSVVKVEGPKGTLSSKMPDGVAMT
                     VRENQLTIERASDDRLARSYHGLARTLVQNMVTGVSAGFEKGLEISGVGYRAELAGST
                     LKLVLGFSSPVEYSIPKGIDIKIEKQVNLVVSGINKELVGRVASEIRALKKPEPYKGK
                     GIKYAGEHIRRKAGKAAGAK"
     gene            complement(1210..1611)
                     /gene="rpsH"
                     /locus_tag="PLR47_07935"
     CDS             complement(1210..1611)
                     /gene="rpsH"
                     /locus_tag="PLR47_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOG12914.1"
                     /translation="MGMTDPIADMLTRIRNGNKARFKTVSVHMSQLNMNIARVLKTNG
                     YIHNYEAVKNDKGHPMLKIALKYPDAKHTVITDIQRVSKPGRRVYVAADQVPKVLNGF
                     GISIISTSRGIMTDQEARESNVGGEVLCKVW"
     gene            complement(1634..1819)
                     /locus_tag="PLR47_07940"
     CDS             complement(1634..1819)
                     /locus_tag="PLR47_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOG12915.1"
                     /translation="MAKKSLIAKAKRKPKFAVREYNRCQICGRPRAYYRKFEMCRICL
                     RKLSLQGELPGVIKSSW"
     gene            complement(1847..2386)
                     /gene="rplE"
                     /locus_tag="PLR47_07945"
     CDS             complement(1847..2386)
                     /gene="rplE"
                     /locus_tag="PLR47_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOG12916.1"
                     /translation="MARLKEYYLKTVVPALVKEFDYKNPMQVPKMEKIVVNMGLGEAI
                     SNVKIIDGAVQEMAMITGQKPVVTKAKKSIATFKLRQGMPIGCSVTLRKDVMYEFFDR
                     LVNVALPKVRDFRGISASAFDGRGNYSIGLHEQIIFPEIEYDKIEKIKGMNITIVTTA
                     RTDEEARALLRLMGVPFKN"
     gene            complement(2393..2716)
                     /gene="rplX"
                     /locus_tag="PLR47_07950"
     CDS             complement(2393..2716)
                     /gene="rplX"
                     /locus_tag="PLR47_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOG12917.1"
                     /translation="MSLGRLKKGDLVKVLAGKDKGKTGKILKAIPEKNKVVVEKINVI
                     KRHKRPDQKSKGGVLEKEAAMDVSKVSIICGKCKEATRIRNRILEDGKKIRICSKCND
                     VIDVG"
     gene            complement(2739..3107)
                     /gene="rplN"
                     /locus_tag="PLR47_07955"
     CDS             complement(2739..3107)
                     /gene="rplN"
                     /locus_tag="PLR47_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOG12918.1"
                     /translation="MIQMQTILNVADNSGAKRVACIKVLGGSKRRFASLGDVIVVAVK
                     EAIPNSKVKKGDVIKAVIVRTVKEVRRPDGTYLKFDDNSAVLISNQMEPIGTRIFGPV
                     ARELRAKQFMKIISLAPEVL"
     gene            complement(3151..3408)
                     /gene="rpsQ"
                     /locus_tag="PLR47_07960"
     CDS             complement(3151..3408)
                     /gene="rpsQ"
                     /locus_tag="PLR47_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOG12919.1"
                     /translation="MAEKSNKRTIKGVVVSDKMDKTIVVKAERLVKHPVFHKYVRRHV
                     KYKAHDEQNQCRIGDTVVIIESRPMSRDKRWRMLEVLEKAK"
     gene            complement(3410..3604)
                     /gene="rpmC"
                     /locus_tag="PLR47_07965"
     CDS             complement(3410..3604)
                     /gene="rpmC"
                     /locus_tag="PLR47_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOG12920.1"
                     /translation="MKISEIRGLGINELQTKKNEMAEELFRLKIRHAGGQLESTATLG
                     RLRRDIARIHTVMKEKEAAG"
     gene            complement(3594..4016)
                     /gene="rplP"
                     /locus_tag="PLR47_07970"
     CDS             complement(3594..4016)
                     /gene="rplP"
                     /locus_tag="PLR47_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOG12921.1"
                     /translation="MLMPKRVKYRKLQKGRMGGKAVRGGTIAFGDYGLQAAECGWVTA
                     RQIEAARVAMTRYVKRGGKIWIRVFPHKSITKKPAETRMGKGKGAPEGWVAVVKPGAV
                     LYEMEGVTKDVAREAFRLAAHKLAISTKFCSRELSDEN"
     gene            complement(4064..>4310)
                     /gene="rpsC"
                     /locus_tag="PLR47_07975"
     CDS             complement(4064..>4310)
                     /gene="rpsC"
                     /locus_tag="PLR47_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOG12922.1"
                     /translation="KCVGYALKFGAKGIKISCAGRLGGAEIARSEWYREGRVPLHTLR
                     ADIDYGFIEAHTAYGLVGVKVLIFHGEVLPQKNEKAS"
BASE COUNT          778 a         1360 c         1016 g         1156 t
ORIGIN      
        1 attcccttgc ggatcgcctc gggcacttcg tgggcttttc ccagtcccgc gccgaccatg
       61 ccctggccgt cgccgaccac cacgatcgcg ctgaaacgaa accgtcttcc tcccttgacg
      121 acctttgccg tgcggttaat ggcaatgacc cggtcgagga gctctacttc cttcatgtct
      181 ctgctgtcaa ttgattcttc tctgatcatg ggttccgttc acatacgtaa ttaaaattta
      241 agaccgcttt cccgtgcccc gtcggccagg gcttttacgc ggccgtgata gagaaaacgc
      301 cctcggtcaa aaacgacttt ctccactccc agcgtctgca ggcgcgccgc aatcagcttg
      361 cccacttccc gggccacctc caccttcttg attccacgaa ttttgccggc cagttccctg
      421 ctcagcgtgg aagccgtcgc gatcgtcttg ccctgcgcgt cattgaccac ctgagcgtaa
      481 atgtgcttgt ccgaccggaa gacagaaagg cggggccgct cggcggttcc cgtcagtttg
      541 cccctgactc tcttttccct tttctctctt accttcagcg tctttttgga agccaatgtt
      601 ccacctcttt tttgctgtgt ttccctaaac gattatttcg cgcccgcagc cttgccggct
      661 ttccggcgaa tatgctcgcc cgcgtacttg atgccctttc ccttgtaggg ttccggcttc
      721 ttcagcgccc ggatctccga ggcgacacgg cccaccagct ccttgttgat gccggaaacg
      781 acgaggttga cctgcttttc gatcttgatg tcaatccctt tgggaatgct gtattcgacc
      841 gggctggaaa atcccaggac cagcttcagg gtcgatccgg cgagttccgc gcggtacccg
      901 acgccggaaa tttcaagtcc cttctcaaaa ccggcgctga cgccggtcac catgttctgc
      961 accagcgtcc gggccaggcc gtgataggac cgggccaggc gatcatcgga cgctctctcg
     1021 atcgtcagct gattttccct gaccgtcatc gcgacgccgt ccggcatttt tgaagacagg
     1081 gtccccttgg gcccctccac ttttacgaca ctgccgctct ggctgacctt cacattcttt
     1141 ggaaacgtga ttggttgttt acctattctc gacatgaata agagctcccg tctaccctct
     1201 gtaattgaac taccaaacct tgcaaagcac ttccccgccg acattcgatt cccttgcttc
     1261 ctgatcggtc atgatgccgc gggacgtgga aataatcgaa atgccgaatc cgttgagcac
     1321 tttgggaacc tggtcggccg caacgtacac cctgcgcccg ggtttgctga ctctctgaat
     1381 atcggtaatc accgtgtgct tggcatccgg atatttgagg gcgatcttga gcatcggatg
     1441 acccttgtca ttcttcacgg cctcgtaatt atggatatat ccgttcgttt tcagcaccct
     1501 ggcaatgttc atattcaact gcgacatgtg aacactgacc gtcttgaagc gggctttgtt
     1561 cccgttgcgt atccgggtca gcatatcggc aataggatcc gtcatcccca tgcttgttct
     1621 ccttacacga tgtttaccag cttgatttga tcacgccggg aagttcgccc tgcagggaca
     1681 gcttcctcag gcagattctg cacatctcga attttctata gtacgcccgg ggccttccgc
     1741 aaatctgaca gcggttgtat tcccggaccg caaatttcgg ttttcttttt gcctttgcga
     1801 tcaacgactt ttttgccacg ctacaattcc tccggagctt cctggtttag ttcttaaagg
     1861 gcacgcccat cagccgcagc agcgcgcggg cctcttcgtc cgtccgggct gtggtcacaa
     1921 tggtaatgtt catccccttg atcttttcga ttttgtcata ctcgatttcc gggaaaataa
     1981 tctgctcgtg aagcccgatg gaatagttgc cccgcccgtc aaaagcgctg gctgatattc
     2041 ctcgaaaatc gcggactttc ggcagggcca cgttcaccag gcggtcaaag aactcgtaca
     2101 tcacgtcttt gcggagcgtc actgaacagc cgatcggcat gccctggcgg agcttgaagg
     2161 tcgcgatgga ttttttcgct ttggtgacca ccggcttctg ccccgtgatc atcgccattt
     2221 cctgcacggc gccgtcaatg atcttcacgt tggagatggc ctcgcccaga cccatgttga
     2281 cgacaatctt ctccatcttc ggaacctgca tgggattctt gtagtcgaat tccttgacca
     2341 gggccggcac tacggttttc aaatagtatt cctttaatct tgccattttt tcctaaccca
     2401 catcgatgac gtcattgcac ttgctgcaga ttcgaatttt cttgccgtcc tcgaggatac
     2461 ggttccgaat ccgggtcgcc tctttgcatt ttccgcagat gatgctgacc ttggagacat
     2521 ccatcgccgc ctctttttcc agaacgccgc ccttgctttt ctgatcaggc ctcttgtgcc
     2581 gcttgatcac attgatcttt tcgacgacca ctttgttttt ctcgggaatg gctttcagaa
     2641 tcttgccggt tttccccttg tccttgccgg ccagaacttt caccaggtcc ccttttttca
     2701 atctgcctaa actcattttt cccctcttca aaaccggctt acaaaacttc tggcgccaga
     2761 gaaatgattt tcatgaactg cttggcccgc aattcgcggg cgaccggccc gaaaattctt
     2821 gtgccgatcg gctccatctg attgctgatg agaacggccg agttgtcgtc aaacttgaga
     2881 tacgttccgt ccggccgcct cacttccttc acggtgcgga cgatgaccgc cttgatgaca
     2941 tcgccttttt tcaccttgga attcggaatc gcctctttca ccgccacgac aatcacatcg
     3001 cccagagaag cgaagcgccg ctttgatccg cccagcactt taatgcacgc gacccgcttg
     3061 gcgccggaat tgtcggcaac atttaaaatg gtctgcatct gaatcataaa tctaccccac
     3121 tgtcacaacc ggcccggacg agcccgtttt ctatttcgct ttctccagaa cttccagcat
     3181 tctccagcgc ttgtcccggc tcatcggccg cgactcaatg atgacgacgg tatccccgat
     3241 ccggcactgg ttttgctcat catgagcctt gtatttcacg tgccggcgga cgtatttgtg
     3301 aaacaccgga tgcttcacca gccgttcggc tttcaccacg atggttttgt ccattttgtc
     3361 gctgacgacc acgcctttga tggtgcgttt gttgctcttt tcagccatgt taacctgcgg
     3421 cctccttttc cttcatcacc gtatggatgc gggcaatgtc cctgcgcagc cgccccaggg
     3481 tcgccgtgga ttccagctgt cccccggcgt gccgaatctt caagcggaac agctcttccg
     3541 ccatttcatt ctttttggtc tgaagctcgt tgattccgag acctctgatt tcgctaattt
     3601 tcatccgaca gctcccttga acaaaacttg gtcgaaatcg ccagtttgtg cgccgccaga
     3661 cgaaaggctt ccctggccac gtctttggtg acgccttcca tctcgtagag caccgctccg
     3721 ggcttcacga ccgccaccca gccttccggc gcgcccttgc cttttcccat gcgggtctcc
     3781 gcgggttttt tcgtgatgga cttgtgcgga aacacgcgaa tccagatttt gccgccgcgc
     3841 tttacgtacc gggtcatcgc gacacgcgcc gcctcgatct gccgggcggt cacccatccg
     3901 cattccgccg cctgcaaccc atagtcgcca aaggcaatgg tgccgccgcg aaccgctttt
     3961 ccgcccatgc ggcccttctg caattttctg tattttaccc gttttggcat taacataagc
     4021 tacaactccc gtttatcttc aacaagccct gcctgcgacg caatcaggac gctttctcgt
     4081 tcttctgggg caacacttct ccgtgaaaaa tcagaacctt gacgccgacc agcccgtagg
     4141 ccgtgtgggc ttcgataaag ccgtagtcga tgtcggcccg cagcgtatgc aggggaaccc
     4201 ggccttcgcg gtaccactcc gagcgggcga tttcggcgcc ccccagccgt ccggcgcagc
     4261 tgattttgat gcctttggcg ccgaacttca gcgcgtagcc gacgcacttt
//
LOCUS       DAOSIM010000179         4310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_18030_length_4310_cov_7.866980, whole genome shotgun sequence.
ACCESSION   DAOSIM010000179 DAOSIM010000000
VERSION     DAOSIM010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18030_length_4310_cov_7.866980"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1627)
                     /gene="metG"
                     /locus_tag="PLR47_07980"
     CDS             complement(<1..1627)
                     /gene="metG"
                     /locus_tag="PLR47_07980"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOG12923.1"
                     /translation="MSRPFYITTPIYYVNASPHIGHAYTTIVADVLARFARMTGRDTF
                     FVTGTDEHGDKIAEAAQKAGITPKAYADGISAQFRGLWPELAITNDYFIRTTDENHME
                     TVRRILQKVYDAGDIYFGSYEGFYCVGCERFYTEKELVDGKCPDHQTEPEHRKESNYF
                     FRMSKYQEWLTGYIQEHPDFIRPERYRNEVLAFLREPLEDLCISRPKTRLEWGITLPF
                     DDQYVTYVWFDALINYVTAVGYPDGGNFKKFWPQAQHLIAKDILKPHGIYWPTMLKAA
                     GIEPYRHLNVHGYWNVDSSKMSKSLGNVVRPLDLKDKYGLDAFRYFLLRDMVFGLDSN
                     FSEEAFVARLNSDLANDLGNLVSRSVTMAIKYCDGKIPAQPDAALEGRLPELAQKTVA
                     EVEACFADMTLHKALMAVWELVGVANKYIVENEPWSLAKDPAQGEKLAAIMYRLLEVL
                     RAVAILISPFMPQAAQKILQTIGLDAQETCDLNAIRSGAPLPAGAALSRGESLFPRVS
                     AEKEAPPPKKPAVELKPEIDYDEFSKVDLRVAKI"
     gene            complement(1647..2423)
                     /gene="ricT"
                     /locus_tag="PLR47_07985"
     CDS             complement(1647..2423)
                     /gene="ricT"
                     /locus_tag="PLR47_07985"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOG12924.1"
                     /translation="MQTNILGVKFKKEGRIYHFDAADLILHKNDQVLVNTDNGVAMGT
                     VVTDVRRSEASELPGSLKNIIRKVTADDLRVREELELLEEEARKYCIGKIAEKGLSMK
                     LITVECLFDRSKMIFYFTADARVDFRELVKDLVSRFKTRIELKQIGARQEARIVKGIA
                     VCGRTVCCAGILQNLDRVTVKMAKEQSMSLNPEKISGLCGRLMCCLSFEHEGYADARK
                     GAREAAKIEPVRESPGSGETPPPARIRTSSPREPKKKRKA"
     gene            complement(2438..3418)
                     /gene="holB"
                     /locus_tag="PLR47_07990"
     CDS             complement(2438..3418)
                     /gene="holB"
                     /locus_tag="PLR47_07990"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOG12925.1"
                     /translation="MSFANIYGHHQQITMLQKAMARKRVGHSYIFSGLAAIGKKTLAL
                     EFARALNCENAGIRQDACGSCPSCRKMTRGNHPDIHVLEAQTQFIRIADIRGIQDQMT
                     FRPLEGRKRVFIIDDADKMNEQAANALLKTLEEPSADNLLILVTARPFWLPQTILSRC
                     LHVRFNPLPAETVARFLTDQKGMDPAGAPALASLSGGSIGRALEMDSEDMIAFRARLS
                     NLLRSAGIGEPMNRLVLASFLGQDKKRLQQGLSILHTYFRDALVYRETARASMVINAD
                     DLPAVESLAGRLKGSQMLHNIAVLENSRQSLDMNVNKSLTLEAMAFKLHL"
     gene            complement(3441..4088)
                     /gene="tmk"
                     /locus_tag="PLR47_07995"
     CDS             complement(3441..4088)
                     /gene="tmk"
                     /locus_tag="PLR47_07995"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOG12926.1"
                     /translation="MNTFITFEGVEGSGKSTQIKRLGDYLERENIPVVLTQEPSGTPI
                     GRKIGDILFHRENVALCPETELLLFLAARAQHVREVILPALQSGKTVLCDRYSDATFA
                     YQAAGRGLDPAFVAAANDYCAMQVKPALTILFDLPVEIGLARAGSRDARRKDPAAADR
                     FEREKIDFHDRVRQAYLNLYAANPERFRLIDASKSEDEIESCVRGHVMKFIHQQQ"
     gene            complement(4066..>4310)
                     /locus_tag="PLR47_08000"
     CDS             complement(4066..>4310)
                     /locus_tag="PLR47_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12927.1"
                     /translation="APAAINARWKRSFPEKKSNIHAPFILGEYPSRPADFPLLFLNVV
                     RPFGAIITLKNGFCTRSSLIQSRDAERSVHEHIHHF"
BASE COUNT          801 a         1227 c         1292 g          990 t
ORIGIN      
        1 gaatcttcgc cacgcgcaga tccactttgg aaaattcatc gtagtcgatt tccggcttga
       61 gctcaacggc cggttttttc ggcggcggcg cttctttttc ggcgctgacg cgcgggaaga
      121 gcgactcacc gcgggaaaga gccgcgcccg ctggcagggg ggcgccgctg cggatcgcgt
      181 tcagatcgca ggtctcctga gcgtccagtc cgatcgtttg caggattttt tgggccgcct
      241 gcggcatgaa cggtgaaatc aaaatggcga cggcgcgcaa aacttccaga agacggtaca
      301 tgatggcggc cagtttttcg ccctgggccg gatctttcgc gagcgaccat ggttcgtttt
      361 ccacgatgta tttattggcg acgccgacga gttcccagac ggccatgagc gctttgtgaa
      421 gcgtcatgtc ggcaaagcag gcttccacct ccgccacggt cttttgcgcc agttccggaa
      481 gtctgccttc cagcgccgcg tcgggctgcg cgggaatctt tccgtcgcag tatttaatgg
      541 ccatggtcac ggagcggctg accaggttgc ccaggtcgtt tgccagatcg gagtttagcc
      601 ttgcgacaaa cgcctcctcc gagaaattgg aatcgaggcc gaacaccatg tctctcagca
      661 gaaaatagcg gaaggcgtcc aggccgtact tgtccttcag atccagcggc cggacgacat
      721 tccccagact cttggacatt ttgctcgagt ccacattcca gtagccgtgg acgttgagat
      781 gccggtaggg ctcgatgccc gcggccttga gcatggtcgg ccagtaaatg ccgtgcggct
      841 tcagaatgtc cttggcgatg agatgctggg cctgaggcca gaattttttg aaatttcctc
      901 cgtccggata accgacggcc gtgacgtagt tgatgagcgc gtcaaaccag acataggtga
      961 cgtactgatc gtcgaacggc agcgtgatgc cccattccag gcgcgtcttg ggccgcgaga
     1021 tgcagaggtc ttccagcggc tcgcgcagga aggccaggac ttcgttgcgg tagcgctccg
     1081 gccggatgaa atccggatgc tcctgaatgt agcctgtgag ccactcctgg tatttgctca
     1141 tgcggaagaa atagttgctt tccttgcggt gctccggttc ggtctggtga tcggggcact
     1201 tgccgtccac cagctccttt tccgtataaa accgctcgca gccgacgcag tagaaccctt
     1261 cataggagcc gaagtagata tcgcccgcgt cgtaaacctt ctgcaggatg cgccggacgg
     1321 tttccatgtg attttcatcg gtcgtgcgaa tgaaatagtc gttggtaatg gccagttcgg
     1381 gccagaggcc gcggaactgc gcgctgatgc cgtccgcgta ggctttcggg gtgatgcccg
     1441 ctttttgcgc ggcctcggcg attttgtcgc cgtgctcgtc cgttccggtg acgaaaaacg
     1501 tatcccgtcc ggtcatgcgg gcaaagcgcg ccagcacgtc ggcgacgatg gtcgtgtagg
     1561 cgtgcccgat gtgcggcgac gcgttgacgt agtaaatagg ggtggtgatg taaaaaggtc
     1621 tggacatgat cgtttgctgc tccttgtcac gctttccgtt tctttttcgg ttcccgcgga
     1681 gaactggtcc tgatccgggc cggaggcggt gtttcgccgc tccccgggct ttcccggacg
     1741 ggctcgattt tggccgcttc ccgggcgcct tttcgggcgt cggcgtatcc ctcgtgttca
     1801 aaggacaggc agcacatcag tcttccgcac aatccggaaa ttttctccgg attcagcgac
     1861 atgctttgtt ctttggccat tttgacggtc acgcggtcca ggttctgcaa aatgcccgcg
     1921 cagcagaccg tccggccgca taccgcgatc cccttgacga ttctcgcctc ctggcgcgcg
     1981 ccgatctgct tgagctcgat ccgcgttttg aatcgggaga ccaggtcctt gaccagctcg
     2041 cgaaaatcca cccgggcgtc cgccgtaaaa tagaagatca ttttgcttcg gtcgaaaagg
     2101 cattccaccg tgatcagttt catggaaagt cccttttcgg cgattttgcc gatgcagtat
     2161 ttcctggctt cctcttccag cagctccagt tcttcgcgga cccgcaaatc gtccgcggta
     2221 accttgcgga tgatgttttt caggctgccg gggagctcgg aggcctccga gcggcggaca
     2281 tcggtgacga ccgttcccat cgcgacgccg ttgtcggtgt tgaccagcac ctgatcgttc
     2341 ttgtgcagga tcaggtcggc cgcgtcgaag tgatagatcc ggccttcttt tttgaatttt
     2401 acgccaagaa tgttggtctg catggacttt gcccttttta cagatgaagc ttaaatgcca
     2461 ttgcttccaa tgtcaacgat ttgttcacgt tcatgtccag cgactgccgg gaattttcca
     2521 gcacggcgat attatgtagc atctgcgagc ccttcaggcg gccggccagc gactcaacgg
     2581 cgggcagatc gtccgcgttg atgaccatgg acgcccgcgc cgtttccctg tacaccagcg
     2641 cgtcccggaa atacgtgtgg aggatcgaca ggccctgctg aaggcgtttt ttgtcctggc
     2701 ccaggaaaga agcgaggacc agccggttca tcggttctcc gatgccggca gaccggagaa
     2761 gattgctcaa tcgggcgcga aacgcaatca tgtcttccga gtccatttcc agcgcccggc
     2821 cgatcgagcc gccggaaaga gaggcgagcg ccggggcccc tgcgggatcc atgcccttct
     2881 gatcggttaa aaaccgcgcg acggtttcgg ccggaagcgg attgaagcgc acgtggaggc
     2941 atcgggagag gatggtctgc ggaagccaga agggacgggc cgtcaccagg atgagaagat
     3001 tgtccgccga cggttcctcc agcgttttca gaagggcgtt ggccgcctgt tcgttcattt
     3061 tgtccgcgtc gtcgatgatg aagacgcgct ttcgtccttc cagcggacga aaggtcatct
     3121 ggtcctgaat gccgcggatg tcggcaatgc ggataaactg cgtctgtgct tccaggacgt
     3181 gaatgtccgg gtgattgccg cgcgtcattt tccggcagga agggcagctt ccgcaggcat
     3241 cctgcctgat gcctgcgttt tcgcagttta gcgcccgtgc gaattccagg gccagcgttt
     3301 tcttgccgat ggccgcaagt ccgctgaaaa tgtaggaatg cccgacccgc tttcgggcca
     3361 tggctttctg cagcatggtg atttgctgat gatgaccgta tatgtttgca aaagacatcg
     3421 gaacgcaacc gccgcgaaaa ttattgctgt tggtggatga atttcatcac gtgcccgcgg
     3481 acgcagcttt cgatttcatc ttcgcttttg gacgcgtcga tcaatcgaaa ccgttcggga
     3541 ttcgccgcgt acaggttcag gtaggcctgc cgcacccggt cgtggaagtc gattttttcc
     3601 cgctcaaagc ggtcggctgc cgccggatct ttgcgcctgg cgtcgcggct gcccgcccgt
     3661 gcaagcccga tttccaccgg cagatcgaaa aggatcgtca gcgccggttt tacctgcatc
     3721 gcgcaataat cgttggcggc cgcgacgaaa gcgggatcca gtccccggcc cgccgcctgg
     3781 taggcaaaag tcgcgtcgga ataccggtcg caaagaacgg tttttccgct ttgcagcgcg
     3841 ggcagaatca cctcccgcac gtgctgggcg cgggccgcca aaaacagcag aagctccgtt
     3901 tccgggcaca gggcgacgtt ttcgcggtgg aagagaatat cgccgatttt acggccgatc
     3961 ggagtgcccg acggctcctg tgtcaggacc acgggaatgt tttcccgctc caggtaatcg
     4021 cccagacgct tgatttgcgt ggattttcca gatccttcga ctccttcaaa agtgatgaat
     4081 gtgttcatga acgcttcttt cggcatcccg ggattggatc aaggatgacc tcgtgcaaaa
     4141 gccgtttttc aatgtgatga tggccccgaa ggggcggacg acgttcaaaa aaagcagggg
     4201 aaaatccgcc ggacgggacg gatattcccc caggataaaa ggcgcgtgga tgttactttt
     4261 cttctccggg aacgatcgct tccaccgggc attgatcgca gcaggcgccg
//
LOCUS       DAOSIM010000180         4291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_18136_length_4291_cov_6.724976, whole genome shotgun sequence.
ACCESSION   DAOSIM010000180 DAOSIM010000000
VERSION     DAOSIM010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18136_length_4291_cov_6.724976"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="PLR47_08005"
     CDS             complement(<1..1501)
                     /locus_tag="PLR47_08005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG12928.1"
                     /translation="MTDFFSRLMRGFQGRGYASTPATLWFLVVFAIAVVLRCIAWSNT
                     AVITPDGAAYIFQAKAIFYGQWKDIADCSAGFKFFTIYPFLVAASYNVCPDWVIAGRS
                     INFTFGVATIIPLYLLLRQFLDYRISALTALIVAVSPFFVGTSVDVLRDPTYWLFAMT
                     GLYLFTVCMKNDNWLFLFFSSLFFLLAAWTKHEAIILPAASFIYLLWKDRHPVKILIF
                     LSPVVAVVALAFIIAGFMNTAVSELNKVDVFVAGFLNSFSSYAALSNELGVLEKSIDG
                     QTQSILKFFLREAQMNIWLVALGMLINRLLEASMYVLMIPFIIGFAKLKRIKEDPRLI
                     YFLFLAAAVLSALYLFIIQTWGLQYRYVAIFTLSSIVFAGIGLETVVKWSSARYQLKE
                     TLVIAILACLMLLSSLPKNMSARDPDKIVFKEIGEFMVKHEGGNNRAISVSSPMEIQR
                     WISFYANLNYQGAVCPEASDQTSWEIFAKQKTLVEQLKQRNIRYFLWTEK"
     gene            complement(1551..2402)
                     /gene="folD"
                     /locus_tag="PLR47_08010"
     CDS             complement(1551..2402)
                     /gene="folD"
                     /locus_tag="PLR47_08010"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOG12929.1"
                     /translation="MSIIIDGNKIAQDIRQAVREEASDLARRTGIVPGLAVVLAGDDP
                     ASKVYVGRKAKACAEVGFLSREYKLPADVGEAELLALIRDLNNDRRIHGILVQLPLPK
                     HIVTDKIIAAIDPNKDVDGFHPANVGRLVTGSPLFIPCTPRGIMELIARTGIELSGKD
                     AVVIGRSNIVGKPMAFLLLAQNATVTMCHSKTKDLPAAARRADVLIAAVGKPHMITAD
                     MVKEGAVVIDVGVNRLENGKLAGDVAFDEVAPKASAITPVPGGVGPMTIAMLMKNTLD
                     AARMAAV"
     gene            2641..4101
                     /locus_tag="PLR47_08015"
     CDS             2641..4101
                     /locus_tag="PLR47_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG12930.1"
                     /translation="MKAVNLALLLTESVQLHADRPALIADNQRITYRQLQSAVSAVAR
                     SLKNMELEKGDKVAIMLPNIPEFVYSCFAVFQSGAVAVLLNTGSTPYEMNYLLNNSDA
                     KILITHSAGEKRYQEIKDQLTTCRNILTVDPAKNAFLSENESGAPELPSVPIDPEDPA
                     VMIYTAGLTGKPLGAVLTHANLCSELDVVHATFRRTPDDVGLCLIPLFHSFGVTVNML
                     NVIQAGCSTVMMDRLTMDSLFSTLEREKISYIVAVPRLYMGMVFYEKAAKYDLSSLKL
                     CVTGGSAMPPDFIPVFEQKFGVRILEGYGLTEAAPACSFNRMETVAKPGSIGTALEGI
                     DIKIVDEDGRELPRDTIGELIVRGGNVMKGYYKDKAATAAVLKDGWLYTGDLGRMDEE
                     GYIFLTGIKKRMIITSGFNVYPREVETILEMHPAVKECRVVGKQDLMRGELVKAQVVL
                     REGFHPDEKDIIRHCKVYLSPYKVPREVEFIPSIGE"
BASE COUNT         1082 a         1148 c         1107 g          954 t
ORIGIN      
        1 ttttttccgt ccataaaaag taccgtatat tcctttgctt gagctgctcc accagtgttt
       61 tttgcttcgc aaagatttcc caactggttt gatcggaagc ttcagggcag acggcgccct
      121 gataattcag attcgcataa aacgatatcc atctctgaat ttccatgggg gaagaaacgc
      181 ttattgcccg gttattgcct ccctcatgct tgaccataaa ttcgccgatt tctttaaaga
      241 cgatcttgtc cggatccctt gcagacatat tcttgggaag cgaggagagc agcatcaggc
      301 aggccaggat tgcaatcacg agcgtctcct tcaattgata tctggcgctc gaccacttga
      361 cgacggtttc cagtccaatc cccgcaaaaa caatggatga caaagtaaat atcgccacat
      421 agcggtactg caatccccat gtttggatga tgaacaaata caatgctgaa agaacagcgg
      481 cagccaaaaa cagaaagtat atcaggcgag ggtcttcctt gattcttttt agctttgcaa
      541 aaccgataat gaaagggatc atcaatacgt acatggaagc ttccagcaac cggtttatga
      601 gcattcccaa tgcaacaagc cagatattca tttgtgcttc ccgtaagaaa aatttcaaaa
      661 tggactgggt ctgtccgtct atgctctttt ccaacacgcc caattcgttg ctgagagcgg
      721 cataacttga aaacgaattt aaaaaaccgg ccacaaatac atccactttg ttcagttcgc
      781 ttacagcagt gttcatgaag ccggcaataa taaaggcaag cgccacgaca gccacaaccg
      841 gcgacagaaa aataaggatt tttacaggat ggcggtcttt ccaaagcaag taaataaaag
      901 atgccgccgg caggatgatg gcttcatgtt ttgtccatgc ggccagcagg aagaaaaggc
      961 tgctgaaaaa caagaaaagc caattgtcat tcttcatgca taccgtgaat aaatacagcc
     1021 ccgtcatcgc aaataaccaa taggtgggat ctctaaggac atccacgctg gtgccgacaa
     1081 aaaacggact taccgcaaca atgagagcgg tcaacgcgct gattctatag tccaggaatt
     1141 gccgcagcag cagataaagc ggaatgatgg ttgcgacgcc aaaggtaaag ttgatggatc
     1201 tgccggcgat aacccagtcc gggcaaacat tataggaggc ggcaaccaag aaaggatata
     1261 tggtgaaaaa tttaaagccg gcactgcaat cggctatatc tttccactgg ccgtaaaaaa
     1321 tggccttggc ttgaaaaata taggcggcgc cgtcgggagt tatcacagcg gtgttggacc
     1381 aggcaatgca gcgcaagaca acggcaatgg caaaaacaac gagaaaccaa agcgttgctg
     1441 gagttgaagc gtatcctctg ccttgaaatc ctctcataag gcgggaaaag aaatctgtca
     1501 aatttcagca cccccatgac aacaaatatt cttgtaccgt atgaggagac ttacactgcg
     1561 gccattctcg ccgcgtccag cgtgtttttc atgagcatgg cgatggtcat ggggccaacg
     1621 ccgccgggca cgggcgtaat agcggatgct ttgggagcga cttcatcaaa cgcgacatcg
     1681 cccgccagtt tgccgttctc caggcggttg acgccgacgt caatcaccac cgctccctcc
     1741 ttgaccatgt ccgcggtgat catgtgcggc tttccgacgg cggcgattaa tacatcggcg
     1801 cgtcttgccg cggcgggcag gtccttcgtt ttggaatggc acatcgtcac cgtggcattt
     1861 tgcgccaata gcagaaacgc catgggcttg ccgacgatgt tgctgcgtcc gatcaccacc
     1921 gcgtcttttc cggaaagctc aatgccggtg cgggcgatca gctccatgat gccccggggc
     1981 gtacacggaa taaacagcgg cgagccggtg acgagacggc cgacgttggc cggatgaaaa
     2041 ccgtccacgt ctttgttcgg gtcgatggcg gcgattattt tatccgtgac gatatgtttg
     2101 ggcaggggaa gctgaaccaa aatgccgtga attcgccggt cgttgttcag atcgcggatc
     2161 agggccagaa gctccgcctc gccgacatcg gcgggcagct tgtattcgcg cgacagaaaa
     2221 cccacctccg cgcaggcctt ggcctttctt cccacgtaaa ctttcgaggc gggatcatcg
     2281 cccgccagca cgacggccag gccgggcacg atgccggtcc ttcgggccaa atcagacgct
     2341 tcttcccgaa ccgcctggcg gatatcctgc gcaattttat ttccgtctat aatgatcgac
     2401 ataacttccc ttattttccg atgccgcccc ggtgcttgcc ggtgtgacgc gccggcatga
     2461 ctgttttgga ttcttttcaa tttctcggcc gcaatttttc agcgcgttgg aatatagtgt
     2521 aaaaacaaaa ccctggcaag gaaaaaaccg ccgcgcggat gaaatcggag cacgcatgga
     2581 tatcttcttg ataaaatgcc gattctctgt taagggagcc tcaaaaaaat gtgggggaat
     2641 atgaaagctg ttaatctggc attgctgctg acagagtccg ttcaactgca cgccgaccgg
     2701 cccgcgctga ttgcggacaa ccagcgaatt acctaccggc agctgcagtc ggccgtttcg
     2761 gccgtcgcga ggtctttgaa aaacatggag ctggaaaagg gagacaaggt ggccatcatg
     2821 ctgcccaata tccccgagtt tgtttacagc tgcttcgccg tttttcaatc cggcgccgtg
     2881 gccgtgctgc tcaacacggg atccacgccc tatgaaatga actatctgct caacaacagc
     2941 gacgccaaaa ttctcatcac gcactcggcg ggtgaaaagc gctatcagga aatcaaggat
     3001 caactgacca cctgccgcaa cattctgacg gtcgatccgg cgaaaaacgc tttcctttcg
     3061 gaaaacgaat ccggagcccc ggagcttcct tccgtgccca ttgaccccga ggatccggcc
     3121 gtcatgattt acaccgccgg tctgaccggc aaaccgctgg gcgcggtgct gacccacgcc
     3181 aatctctgct cggagctcga tgtggtccat gccacttttc gaagaacacc cgacgatgtg
     3241 ggcctctgcc tgattcccct tttccattcc ttcggcgtca cggtgaacat gctcaacgtc
     3301 attcaggccg gctgctccac ggtgatgatg gaccggttga cgatggatag tcttttttcc
     3361 acgctcgaaa gagaaaaaat atcgtatatc gtcgccgtcc cccgcctcta catgggcatg
     3421 gtcttttacg aaaaagctgc caaatatgat ctcagctcgc tgaagttatg cgtcaccggc
     3481 ggctcggcca tgccgcccga tttcatccct gtttttgaac agaaattcgg cgtccgcatt
     3541 ctggaaggct atggtctgac ggaagcggcg ccagcctgtt ccttcaacag aatggaaacc
     3601 gtggcaaaac ccggttccat cggaaccgcc ctggaaggca tcgacatcaa aattgttgac
     3661 gaggacggac gggagctgcc gcgcgatacg atcggcgaac tgattgtccg gggcggcaac
     3721 gtcatgaaag gctactataa agacaaagcg gccaccgcgg ccgttctgaa ggacggctgg
     3781 ctttacaccg gggacctggg ccgaatggac gaggaaggct acattttcct gactggcatc
     3841 aagaagcgga tgatcatcac cagcggtttc aatgtctatc ctcgggaagt ggaaacgatt
     3901 ctggagatgc atccggcggt gaaggaatgc cgcgtcgtgg gcaagcagga cctgatgcgc
     3961 ggcgagctgg tcaaggcgca ggttgtcctg cgcgagggat ttcatccgga cgaaaaagac
     4021 atcatccgcc actgcaaggt gtatctctct ccctacaaag tcccccggga agtggaattc
     4081 atcccgtcga ttggcgaata aaaccaaaca gccgggcacc acgcccggct gtttcttcca
     4141 tccaccacag cgggtctgaa gaaaggctgt tgcccaaacg atatcccttt gagcggccat
     4201 tgagagcgtt ttgttatata aatcttagcg aagcgtaaga tcagcaatga caactgtttg
     4261 agccgaaggc gagttttgtc attgcctgaa g
//
LOCUS       DAOSIM010000181         4181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_18763_length_4181_cov_7.811440, whole genome shotgun sequence.
ACCESSION   DAOSIM010000181 DAOSIM010000000
VERSION     DAOSIM010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18763_length_4181_cov_7.811440"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1067)
                     /gene="mdh"
                     /locus_tag="PLR47_08020"
     CDS             complement(135..1067)
                     /gene="mdh"
                     /locus_tag="PLR47_08020"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOG12931.1"
                     /translation="MKQKVTVVGAGNVGATAAQRLAEKELADVVLIDIVEGVPQGKSL
                     DLAQAAPIEKHDAHLAGANDYEASAGSDIVIITAGIPRKPGMSRDDLLATNRGIMKNV
                     VRQVVRHSPGAILIIVSNPLDAMCHVAMEESGFPKNRVIGMAGVLDSARFRAFIAAEL
                     DVSVENVQAMVLGGHGDTMVPLPRFSTVAGIPITEWMTPERIESLVARTRSGGAEIVG
                     LLKTGSAYYAPASAAVEMAESILKDKKKILPCAAYLEGEYGFSGLFVGVPVKLGKNGI
                     EQIISLRLTPEEQKALEKSAAAVRELVEAMKKMA"
     gene            complement(1207..1758)
                     /gene="cobU"
                     /locus_tag="PLR47_08025"
     CDS             complement(1207..1758)
                     /gene="cobU"
                     /locus_tag="PLR47_08025"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOG12932.1"
                     /translation="MISKIPENGMTLILGGARSGKSTFAEQTARESGKPVLFIATAAA
                     GDEEMAERIRRHRASRPAGWQTLELPMNVGRSLETTEAGVVIVDCITLLVSNILLALP
                     ENAPEDTVMEEIRAEMDNLIAAQARLGGRWLVVSNEVGLGLVPPYPLGRLYRDALGRA
                     NQILAAAASRVIFLVAGLPLTVK"
     gene            complement(1812..2612)
                     /gene="cbiR"
                     /locus_tag="PLR47_08030"
     CDS             complement(1812..2612)
                     /gene="cbiR"
                     /locus_tag="PLR47_08030"
                     /inference="COORDINATES: protein motif:HMM:NF041277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobamide remodeling phosphodiesterase CbiR"
                     /protein_id="HOG12933.1"
                     /translation="MTYPFRLGATSYVVPADILTNVRYLAGKVLDVELVLFEVDDGQN
                     NLPGKAEIAELAAIARGCDLTYTVHLPLDLRLADDGFPRHASLDKARRVIDGTLALNP
                     WAYVLHLDGKAVLNLSDPESLLRWRDQAVRALELVGQWAGGPRRLAVENLEGYPPDFY
                     DPVLERIAVSRTVDVGHLWLDGCDPVAYLKNALLRTRVIHIHGIEGRDHRSLAKAPRE
                     KLKAVLDELVRSEYRGVVTLEVFSEVDFLTSCAAVKAVMHADMPSPEQ"
     gene            complement(2681..3400)
                     /gene="cobS"
                     /locus_tag="PLR47_08035"
     CDS             complement(2681..3400)
                     /gene="cobS"
                     /locus_tag="PLR47_08035"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOG12934.1"
                     /translation="MQNLRTAFGLLTTLPVGISETWQPGDSGRAAFWYPVVGLAVGGL
                     VWLAWLGLNLWFTPLVSAVLALGVWVGLTGGLHLDGLADCCDALPASVVVERRLEILK
                     DPHLGTFGGIGLIIVLFAKAAALSALAPSQGLGIVLAATLGRWLILPASRLPQARPGG
                     MGADFAAGVRPAAVWITATLPISLAVLLGMQGAFAVLAALISGAALLRLAWKRIGGVT
                     GDVLGALVEVAETAVLLTFTV"
     gene            complement(3437..>4181)
                     /gene="cobT"
                     /locus_tag="PLR47_08040"
     CDS             complement(3437..>4181)
                     /gene="cobT"
                     /locus_tag="PLR47_08040"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF000996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOG12935.1"
                     /translation="AATHIVDIGVASDFDPSLPGLWRRKVARGTSNMAKGPAMTRAQA
                     ERALACGMEVLAEIAEKGLDVVATGDMGIGNTTASSAIVAVMTGLPAARVTGRGTGLD
                     DEGLARKIQVIEQAVAVNQPDPNDAMDVLCKVGGLEIAGLAGVMIAAAARRIPVAVDG
                     FISTAAAMIGVGLVPDLRHYLFGAHRSVEIGHRAMHRHLGLAPLLNLSLRLGEGTGAA
                     LAFHLMEAACRILREMATFEEAGVSGKGL"
BASE COUNT          838 a         1344 c         1211 g          788 t
ORIGIN      
        1 tcaaacagtt gtcattgctg atctttcgct tcgccaagat ttatataaca aaaccctctc
       61 aatggccgct caaagggata tcgtttgggc aacagccctt tgatttgtaa gcctgctttc
      121 gcgggatcgc cgggtcaggc catctttttc atcgcttcca ccagttcccg gacggcggcg
      181 gcggatttct ccagggcctt ttgctcctcg ggcgtgagtc gcagcgaaat aatctgctcg
      241 atgccgtttt tacccagttt gaccggcacg ccgacaaaca aaccggaaaa gccgtattcc
      301 ccttccagat aagcggcgca gggaagaatt ttcttcttgt ctttgagaat ggattccgcc
      361 atttcgacgg ccgccgaagc aggcgcatag taggcgctgc cggttttcaa aaggccgacg
      421 atttccgctc cgccgctgcg cgttcgcgca accagcgatt cgatccgttc gggcgtcatc
      481 cattccgtga tcggaatgcc cgccactgtg gaaaaacgcg gcagcggcac cattgtgtcc
      541 ccgtggccgc ccaggaccat cgcctgtacg ttttctacgg aaacgtccag ctccgcggcg
      601 atgaaagccc gaaagcgcgc cgaatccaga acgccggcca tgccgataac gcggtttttg
      661 ggaaagccgc tttcttccat cgccacatgg cacatggcgt cgagcggatt gctgacaata
      721 atgaggatgg cgccggggga gtggcggacc acctggcgta cgacattttt catgatgccg
      781 cggttggtcg ccagcaagtc atcgcggctc atgcccggct tgcggggaat gcccgccgtg
      841 atgattacga tgtcggagcc ggcggaagcc tcgtagtcgt tggcgcccgc aaggtgcgcg
      901 tcgtgctttt caatcggcgc ggcctgagcc aggtccagag atttgccctg cggcacgcct
      961 tcgacaatat cgatcaatac cacatcggcc aattctttct ccgccagcct ttgcgccgcc
     1021 gtcgccccga cattgccggc tcccacaacg gttactttct gtttcatgtt gcccatttcc
     1081 tttattggtt gattgaattt gcaacggttt tttcacctac acccaaaaga caaataaatc
     1141 aattgctgcg gtggaatcat cgttttggcg gcgtaaaacg cgcctcgaaa aagcccgcgg
     1201 cgctcgctac ttcaccgtga gcggaaggcc cgcaaccagg aagatcaccc gtgaggccgc
     1261 cgcggccagg atctggttgg cgcggcccag cgcgtcgcgg tagaggcgcc ccagcggata
     1321 gggcggcacg aggcccaaac ccacttcatt cgacacgacc agccaccgcc cgcccagacg
     1381 ggcctgcgcg gcaatgagat tgtccatttc ggcgcgaatt tcttccatga cggtgtcttc
     1441 gggcgcgttt tcgggcaggg ccaggaggat gttgctgacc aggagcgtta tgcaatcgac
     1501 aatgaccacg ccggcttcgg tcgtttcgag gctgcggccg acattcatcg gcagttcgag
     1561 cgtctgccag ccggcggggc gcgaggcccg gtggcggcgg attctttcgg ccatttcctc
     1621 atcgcccgcg gcggcggtgg cgataaaaag tacgggcttt cccgactcac gggcggtttg
     1681 ttctgcaaaa gtgcttttac cgctgcgggc gccgcccagg atgagtgtca tgccgttttc
     1741 cggaatttta gaaatcatgg atgttttcct tgagcacaag agtccttgcc gcctgccggg
     1801 gcggctgcca tctactgttc gggagagggc atgtccgcat gcatcaccgc ttttacggcc
     1861 gcgcacgagg tcagaaaatc aacctcggaa aaaacctcca gcgtcaccac accgcggtat
     1921 tcggagcgga ccagttcgtc cagaacggct ttcagcttct cccgtggcgc ctttgcaaga
     1981 gagcggtgat cgcggccctc aatgccgtga atgtgaatca cgcgcgttcg cagcagcgcg
     2041 ttttttaaat acgcgaccgg atcgcagccg tcaagccaca gatgaccgac atcgacggtc
     2101 cggctgacgg cgatccgctc cagcaccgga tcgtaaaaat ccggcggata gccttccaga
     2161 ttttccacgg ccagacgccg cgggccgccg gcccattggc cgacaagctc aagtgcacgg
     2221 acggcctggt cccgccagcg aagcagactt tccgggtccg aaaggttcaa aacggctttt
     2281 ccgtccagat gcaggacata cgcccaggga ttaagagcca gcgtgccgtc gatgacgcgg
     2341 cgggccttgt ccagggaagc gtggcgcggg aaaccgtcat cggccaggcg caaatccagc
     2401 ggcagatgga ctgtgtaggt caaatcacaa ccgcgggcga tggcggcaag ctctgcaatt
     2461 tcggctttcc cgggcaggtt gttctggccg tcatcgacct cgaagagcac cagttccaca
     2521 tccagcactt tcccggccag atagcggaca ttcgtcagga tgtccgcggg aaccacgtag
     2581 gaggttgcac ccagtcgaaa aggatatgtc atgttgctcc ttaaaaacac cgccgccatt
     2641 ttgccccacc cgctctatca tcagggggaa tcgccttgtt tcatacggtg aaggtcaata
     2701 aaaccgccgt ttcggccact tcaaccagcg cgcccagtac gtctccagtc accccgccga
     2761 tgcgcttcca ggccagacgc agcaatgccg ctcccgaaat cagcgcggcc aggacagcaa
     2821 acgcgccctg cattcccagc aaaaccgcaa gactgatcgg caatgtcgcc gtaatccaaa
     2881 ccgcggccgg ccgaaccccg gccgcaaaat cagcgcccat gccgccgggg cgcgcctgcg
     2941 gcagccgcga ggcaggcagt atgagccagc ggccaagcgt cgccgcaagg acaatcccca
     3001 agccctggga aggcgcgagg gcactgagcg ccgcggcttt ggcgaaaagc acgatgatca
     3061 aaccgatgcc gccgaacgtt cccagatgcg gatccttaag aatttccagg cgtctttcca
     3121 cgacaacgga agcgggcaga gcgtcgcagc agtcggccag gccgtcgagg tgcaggccgc
     3181 cggtaagtcc gacccagact ccaagcgcaa gcacggcgga aacaagcggc gtaaaccata
     3241 ggttcagtcc gagccaggcc agccagacca ggccgcccac cgcaagcccg accaccggat
     3301 accagaaggc ggcccgtccg ctgtctcccg gctgccaggt ttccgatatg ccgacaggca
     3361 gcgtagtcag caatccgaaa gccgtacgca aattctgcat atagttcctt ttatccttca
     3421 gtcttcagcc ttcagtctat aaacctttcc cgctcacgcc tgcctcttca aacgtcgcca
     3481 tttcccgcag aatgcggcag gccgcttcca tcaggtgaaa cgccagcgcc gcgcccgtgc
     3541 cttcgccaag ccgcaggctg aggttcagca ggggggcaag accaagatgc ctgtgcatgg
     3601 cccgatgccc gatctccacg gaccggtgcg cgccgaaaag ataatggcgc agatccggca
     3661 caagcccgac cccgatcatc gcggcggccg ttgaaatgaa gccgtccacc gcgacgggga
     3721 tgcggcgggc ggcggcggcg atcatcacgc cggccagacc cgcaatttcc agcccgccga
     3781 ctttgcaaag cacgtccatc gcatcgttcg ggtcgggctg gttgacggcg acggcctgtt
     3841 cgatcacctg tatcttgcgc gcgagacctt catcgtcaag gcccgtgcct cgcccggtga
     3901 cccgggccgc cggcaatccc gtcatcacgg cgacaatggc cgaggacgcg gtcgtgttgc
     3961 cgatgcccat gtcgccggtg gcaaccacat cgaggccttt ttcggcaatt tcggccagca
     4021 cctccatgcc gcaggcaagg gcccgctcgg cctgggcgcg ggtcatggcc ggtccctttg
     4081 ccatattgct cgttccgcgc gccactttcc gccgccagag tccgggaagg gacggatcaa
     4141 aatcggaagc gacgccgata tcgacaatat gcgtcgcggc g
//
LOCUS       DAOSIM010000182         4129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_19067_length_4129_cov_5.098675, whole genome shotgun sequence.
ACCESSION   DAOSIM010000182 DAOSIM010000000
VERSION     DAOSIM010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19067_length_4129_cov_5.098675"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PLR47_08045"
     CDS             <1..542
                     /locus_tag="PLR47_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOG12936.1"
                     /translation="VEGTFKRYYGTYGKHTGDGLLYYFLKERDNQYMMNAIACALEIR
                     ENMKKLSMDWKMRKGWFNELCLNIGINEGQEYFGTIPSSPNIEFTALGDTVNFAGRLS
                     DFARFGAIWATKNLINKLSVEDRSGIRFGIRHRENDREILIENSFGRVMDMIVPDSQN
                     FNKFMDIATLAITEVVEKR"
     gene            complement(886..2649)
                     /locus_tag="PLR47_08050"
     CDS             complement(886..2649)
                     /locus_tag="PLR47_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG12937.1"
                     /translation="MNTESSNPTSKATQKKLFGEGEFSSLAEAILSSAGVGIYIVQNG
                     TFVYVSPLYQKLTGYSEKELIGTRSLGYIHPEDRAAVREKAIRSLKTKSLKPYEYRFV
                     KKHQEIMWALETITPISYEGQRATLGSFMDISHRKKVETALQKSEENYRLLFESAGQG
                     ILIAKDETIQFANAALADIVGYPKKVITTRPFISFIHPDDRKIVLNRHKMRLKGEIPE
                     NDYQFRAVTSNGTVKWLQINARIITWDGVPASLSFITDITDRRQAQEELRASEEKYRT
                     IFENAQEGIYQTTCEGKFIMANQAMARILGYDAPEELLDAQSDLARRIYVNPEDRTKV
                     IEQVQREGVVRGQEIQFYRRDGRKIWISRTMRIVRDDQNNEPYLEGIVEDITEKKESV
                     DRLRKALGGTVQAIASVVETKDPYTAGHQRRVADLARAMGTEMHLSSDQIEGLRMAGI
                     IHDIGKISVPAEILSTPRRLTAIEFSLIKMHAQSGFDILKDVDFPWPIARMVLEHHER
                     MDGSGYPNGLTGNDILLESRVLAVADVVEAMATHRPYRPALGLEAALEEINSKKGILY
                     DPQVVSVCQRLFQDKNFVIKA"
     gene            2816..3034
                     /locus_tag="PLR47_08055"
     CDS             2816..3034
                     /locus_tag="PLR47_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12938.1"
                     /translation="MTVKKETTKKRAVKKASPAAKRAAKPDLKEFLEEVQQKAYELYQ
                     DRLRSGVAGDEISDWFEAEKAIKEKYHL"
     gene            3085..>4129
                     /gene="glgC"
                     /locus_tag="PLR47_08060"
     CDS             3085..>4129
                     /gene="glgC"
                     /locus_tag="PLR47_08060"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOG12939.1"
                     /translation="MDANQEQADGQNYYGYVKTKIATEVTRQTLVLILAGGEGSRLGE
                     LTKWRAKPAVPFGGKYRMIDFPLSNCVNSGLRRIGVLTQYKAHSLIRHLQQAWSFMRA
                     EIGEFVEIIPAQQRMSKEWYRGTADALYQNIDIIHRHAPEYVLVLGGDHIYTMDYSKM
                     LMQHVNSKADMTVACIEVPRMEACDFGVMSVDESMRISRFTEKPADPEAMPGKPDLAL
                     ASMGIYVFSTRFLYDSLIEDARDENSAHDFGRSIIPRAIHNSVAMAYPYRDEQGKMAY
                     WRDVGTVDAYWKANMELCAVEPELNLYNQKWPIWTYQTQHPPAKFIFDDEGRRGEAIN
                     SLVSGGCIIAGARV"
BASE COUNT          985 a         1085 c         1071 g          988 t
ORIGIN      
        1 gcgtggaagg cacattcaag cggtactacg gcacctacgg caagcatacg ggcgatgggc
       61 tgctttatta ttttctcaag gagcgggaca atcaatacat gatgaacgca attgcctgtg
      121 cgctggaaat acgcgaaaac atgaaaaaat taagcatgga ctggaaaatg cgcaaaggct
      181 ggttcaatga attgtgcctc aatatcggca ttaatgaggg tcaggaatac ttcggcacta
      241 ttccttcatc ccccaatatt gaatttaccg ctctgggaga caccgtcaat tttgccggac
      301 ggttatccga ttttgcccgg ttcggcgcga tctgggcaac aaaaaatttg atcaacaagc
      361 tttccgtgga agatcgttcc ggcattcgct ttggaattcg gcacagggaa aacgacaggg
      421 agatactgat tgaaaactca ttcggccgcg tcatggacat gatcgtgcct gatagtcaaa
      481 atttcaacaa attcatggat attgccacac tggcgatcac cgaagttgtg gaaaagcgct
      541 agaatcctga catctgctca ttccgaatcc ggcagatcgt aaattttacg ccgaaggctt
      601 agcaaaaagc ataaaacgtc aaaaatattt cgcaaaatag ctgaagaatc atcggcattt
      661 tctgttgaaa acattaacag cgcctcaagt atttccacag acttatcaac aactgctcaa
      721 cagggttgca aacaagcaat tccacaaggg gcaagttggt ggatcgtgat ttttctcgac
      781 aagaaagttt ttttgactga aaagttatct tgacgattgt tttgttattt caaattatta
      841 agattaatca atcagttttt actttaatga tgggcattcc ctgattcatg ctttgatgac
      901 aaaattctta tcctggaaaa gcctctggca gacagatacc acttgcggat cgtagagaat
      961 ccccttcttt gagttgattt cctccagcgc ggcttccagc cccagggcgg gccggtaggg
     1021 acggtgcgtc gccatggctt ccaccacatc ggccacggcc agaactctcg actccagaag
     1081 aatgtcgttc ccggtcaaac cattcggata tccggacccg tccatccgct catggtgctc
     1141 cagaaccatt ctggcgatgg gccagggaaa atccacgtcc ttcaggatgt caaagccgga
     1201 ctgggcatgc atcttgatta ggctgaattc aatggcggtc agacgccggg gcgtactcag
     1261 aatctcggcc gggacggaaa tcttgccgat gtcgtggatg atgccggcca ttctcagtcc
     1321 ctcgatctga tccgaactca ggtgcatttc cgtgcccatg gcccgggcca ggtcggccac
     1381 ccttctctgg tgtccggcgg tatacggatc tttggtttcg acgacggagg caatggcctg
     1441 aacggttccg cccagggctt tgcgcagacg gtccacgctt tccttttttt cagtgatgtc
     1501 ttctacgatg ccttccaggt agggctcatt gttttgatca tcgcggacga ttctcatggt
     1561 gcgggatatc cagattttcc gtccgtccct tcggtaaaac tgaatttcct gcccccggac
     1621 gaccccttcc ctctggacct gttcaatgac ttttgtccga tcttcgggat tgacgtaaat
     1681 ccgccgggca aggtcggact gtgcatcaag cagttcttcc ggcgcgtcgt atcccaggat
     1741 cctcgccatg gcctgattgg ccatgatgaa tttcccttca catgtagtct ggtagatccc
     1801 ctcctgggcg ttttcaaaga tggtgcggta tttttcttcg cttgcgcgaa gctcttcctg
     1861 agcctgtctt cggtcggtga tgtccgtgat gaagctcagg gaggcaggca cgccgtccca
     1921 ggtaatgatc ctggcgttga tttgcagcca cttgaccgtc ccattcgagg taacggcccg
     1981 aaactgataa tcgttttccg ggatttcccc cttcaatctc attttgtgcc ggttcagaac
     2041 gatcttacga tcatcgggat ggataaaaga tataaacggg cgggtggtga tgactttttt
     2101 agggtatccg acaatatcgg cgagggcggc attggcaaac tgaatggtct cgtctttggc
     2161 aatcaggatt ccctgccccg cactttcaaa aagcagacgg taattttctt cgcttttctg
     2221 aagcgccgtt tccaccttct tgcggtggga gatgtccatg aagctcccca gcgtcgccct
     2281 ttgcccttcg tacgagatgg gcgtgatggt ctccagagcc cacatgatct cctggtgctt
     2341 cttgacgaac cggtactcat agggtttaag gcttttcgtt ttgaggcttc ggatcgcttt
     2401 ttccctgacg gccgcccgat cctccgggtg aatgtaaccc agagaacggg tgccgatcag
     2461 ttccttttcg gaataaccgg tcaacttttg gtacagtggg ctgacgtaga caaacgtgcc
     2521 gttttgaacg atgtaaattc cgacgccggc gctggataaa atggcctcgg ccagagatga
     2581 aaattcgccc tcgccaaaaa gctttttttg ggtcgccttt gatgttggat tgctggactc
     2641 agtattcaag aatttcccgc cttatcgtga gtgatttcga aaacatcatt tgttcatcaa
     2701 tggggctttc atacacagaa taatgcttca tttcaagtaa aatacaggca ttgacggccg
     2761 gccggcaaaa gtgctattga aacagcgtct gaaaacatca accgaggagg cgatcatgac
     2821 ggtcaaaaaa gaaaccacca aaaaacgcgc ggtcaaaaaa gcatcgcctg cggcaaaaag
     2881 ggcggcaaag cctgacctga aagaatttct ggaagaggtc cagcaaaaag cttacgagct
     2941 ttaccaggac aggctccgct ccggcgttgc cggcgacgaa atatccgact ggtttgaagc
     3001 cgaaaaagcg atcaaagaaa aatatcatct gtagcgttgt actgatcctg atggaaacca
     3061 cttaataacc ggggttgaaa cgtcatggac gcaaatcaag aacaagcgga cgggcagaat
     3121 tactacggct atgtgaaaac caagattgcc actgaggtca cccggcaaac gctggtgctg
     3181 atcctggcgg gcggcgaggg atcccgcctg ggcgaactga ccaaatggcg cgccaaaccg
     3241 gcggttcctt tcggcggcaa ataccgcatg atcgattttc ccctgtcgaa ctgcgtcaac
     3301 tccggcttgc gccgcatcgg cgttctgacc cagtataagg cgcactcgct gatccgccat
     3361 ttgcagcagg cctggagctt catgcgcgcc gagatcggcg agttcgtgga gatcattccc
     3421 gcccagcagc gcatgagcaa ggaatggtat cgcggaacgg ccgacgcgct ttaccagaat
     3481 atcgacatca ttcaccgcca tgcgcccgaa tacgtgctgg tgctgggcgg cgatcatatc
     3541 tacacgatgg attactccaa gatgctgatg cagcacgtca attcaaaagc cgatatgacg
     3601 gtggcctgca tcgaggtgcc gcgcatggag gcgtgcgatt tcggcgtcat gtcggtcgat
     3661 gaaagcatgc gcatctcccg cttcaccgag aaaccggccg atccggaggc catgcccggc
     3721 aaaccggatc tcgccctggc ctccatgggg atttacgtct tctccacacg ctttttgtac
     3781 gattccctga tcgaagacgc cagggatgaa aactccgccc atgatttcgg aagaagcata
     3841 attccccgcg ccatccacaa cagcgtggcc atggcgtatc cctaccgcga cgagcagggc
     3901 aagatggcct actggcgtga cgtggggacc gtagatgcct actggaaggc caatatggag
     3961 ctttgcgccg tcgagcctga gctgaacctg tataatcaga agtggccgat ctggacctac
     4021 cagacgcagc atccgcccgc gaagttcatc tttgacgacg aaggacggcg cggcgaagcc
     4081 atcaactcgc tggtcagcgg cggctgcatc atcgccggcg cccgcgtca
//
LOCUS       DAOSIM010000183         4111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_19166_length_4111_cov_6.265779, whole genome shotgun sequence.
ACCESSION   DAOSIM010000183 DAOSIM010000000
VERSION     DAOSIM010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19166_length_4111_cov_6.265779"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..883)
                     /locus_tag="PLR47_08065"
     CDS             complement(173..883)
                     /locus_tag="PLR47_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12940.1"
                     /translation="MKEFDEESLAQFDGRDGRPCCIAHGDRVIDVSASKLWKTGSHMK
                     RHQAGSDLTLAIGAAPHGTDVLDKYPQIGIFKKKNAKPFLPAPLEALMERVPFLRRHP
                     HPMVVHFPMVFSMAPVFFYLLYLLTGERSFEATALHCLAADILFIVPAIATGFLTWWV
                     NYQAKFMKPVRIKIYASFILLAVAATAFLWRILRPEAFAAFGTEAVIYFILLLSMAVL
                     VSIIGFFGGGLTFPAEKK"
     gene            1379..2764
                     /locus_tag="PLR47_08070"
     CDS             1379..2764
                     /locus_tag="PLR47_08070"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG12941.1"
                     /translation="MGDRMFIMFVHLVQNLAVLMVMAYVTTRIPWFGDLLNRRFSLKN
                     SLLLGLVFGLISIFGTLSAVKTFGALANFRDLGPAIAGLLAGPVAGGVAGLIGGMHRY
                     SLGGITGVPCSIATVLAGLICGGFYLWKKGRPIRIREATAVMAVVTILHGFVITPLIL
                     GLSEEIRQILRTVMLPMLLVNCAGIALFFFILHNLNRERLNEAEKHRIDSELRIAREI
                     QMGMLPAPSQNGDPRFAVHAVLKPAKEVGGDLYHFFAKDNDRICLAVGDVSGKGISAS
                     LHMAITQKLIKALALSGEEPAELLGRLNRELCDGNESMMFVTLFVAFIHLESGKTVFG
                     NGGHNPPYRIRRGDVSRMVLEPGLALGIRENAVYTNQRLCLEAGDTLFLYTDGVTEAM
                     NGRKELFGEKRLAAALSRLRDSDPEGICRGLLEELAHFVGQSEPSDDITMLAVRLTEK
                     SNAAAGAVRGG"
     gene            2802..3134
                     /locus_tag="PLR47_08075"
     CDS             2802..3134
                     /locus_tag="PLR47_08075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG12942.1"
                     /translation="MKVWSKRQQDVVVLAVQGRLDAATAPLFEEQVLPMISAGDKNFL
                     LDLAGLDYISSAALRRLLFFAKRAEDQGGRVVLLSVQNHILEILDMAGFTQLFAIFAD
                     QDEALRSF"
     gene            3146..>4111
                     /locus_tag="PLR47_08080"
     CDS             3146..>4111
                     /locus_tag="PLR47_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOG12943.1"
                     /translation="MPRKPSNLIYGVDENPGPIALVLMGLQHISLMSIAFIFPVIIIE
                     AIGGNLEEAEGLIRMAMLATGIGTILQGINRGPVGSGYLCPLLNGPAFLSASLMAGKL
                     GGLSLIFGMTAVAGVFEAFFSRLLSRMRAVFPAEVTGTIVTMVGIEVVPLSVRKFLGI
                     DPTHSMPDSTAVLIAMITLGAMACFNVWGKGKLRLYSVLLGMIVGYAASAMAGLLSPD
                     TFRVMMEAPFFALPAVGHYGFSFEWVLLVPFLVAALSSALKTMGDVTTCQKINDAQWK
                     RPDMVNISGGVLACGISNILSGMLGALGQSVSSSNIGLSIATGATS"
BASE COUNT          788 a         1153 c         1247 g          923 t
ORIGIN      
        1 cagccctttc accgtgaccg tgcgggtgtc cgacttgaag cgggcgacgc cttttccgat
       61 gaaatatccc ccgaggagaa gccccgccag caggcagacg ccgacgatga cggcggccgg
      121 aaccaaggaa cggttttcat gagtggtcat agtttttcct ccttttaaaa ggttattttt
      181 tttccgcggg aaacgtcaaa ccgcctccaa aaaatccaat gatggagacc agcacagcca
      241 tcgaaagcag gagaataaaa tatatgacgg cttccgtccc gaaagcggcg aaagcttccg
      301 gacgaagaat tcgccagaga aacgcggtgg cggcgacggc cagaaggata aaggaggcgt
      361 atattttaat gcggacgggt ttcatgaact ttgcctgata attgacccac caggtcaaaa
      421 aaccggtggc gatggccggc acgatgaaaa gaatatccgc cgccaggcag tgcagggccg
      481 tcgcctcgaa cgaccgctcg ccggtcaaaa gatagagaag atagaaaaac accggcgcca
      541 tggagaagac catgggaaaa tgaacaacca tcgggtgcgg gtggcggcgc agaaaaggaa
      601 cgcgctccat gagcgcttcc agaggcgcgg gcaaaaaagg ttttgcgttc ttctttttaa
      661 aaattccaat ctgcgggtat ttgtccagaa cgtccgtccc atggggcgcg gccccgatgg
      721 ccagggtcag atcgcttccg gcctgatggc gcttcatgtg agaccctgtt ttccagagct
      781 tgcttgcgct gacatcgatc accctgtctc catgcgcaat gcagcagggc cttccgtccc
      841 ggccgtcaaa ttgagccagg gattcttcgt cgaattcttt catggcgcct ccttcttctg
      901 gaatctgatt ccgggccggc cggcaaagcc gagggccttt tgccgcccca cgggttgatt
      961 tgaatatcct cggcgcaggc agggcagaag gctttggggt ccacatggcc gcatccgtag
     1021 ccccaaaggt caaattttct gcactcccgg cacaggggcg cgtcgcagcc gtcacaatag
     1081 acgactgcgt cattttttcc gcaaacgcgg cagctcgttt ttgtttcgat gattctcagt
     1141 gcaaggctcc gatgcggttc tccgccgcta aaattggtaa cgcaagccgg ccgcaagtcc
     1201 atgggatctc aggcgggact cgatgccgtt cacagcgacg gcggctttat aacacacttt
     1261 tttcaaaaca gacatcggga agatgcgatt gcgccttgcg ggggctgaaa attcatatta
     1321 tgatgcccac gtttttcagc ggcggctcgc cgacggaaac ctggaagagg ggttgtttat
     1381 gggggacagg atgttcatca tgttcgtgca cctggttcag aacctcgcgg tgctgatggt
     1441 gatggcctat gttacgaccc gcattccctg gttcggcgat ttgctgaatc ggcggttctc
     1501 tttgaaaaac agtctgcttc tgggtttggt tttcgggctg atttcaattt tcgggacgct
     1561 cagcgccgtg aagacgttcg gcgcgctggc caacttccgc gatctggggc cggcgattgc
     1621 gggcctgctg gccggtccgg tcgcaggggg agtggccggc ctgatcggcg gcatgcaccg
     1681 gtattctctg ggagggataa cgggcgttcc ctgcagcatt gccacggttc tggccggact
     1741 gatctgcggc ggtttttatc tgtggaaaaa gggccgtccg atccggatcc gggaggcaac
     1801 cgccgtcatg gcggtcgtca cgattctgca cggctttgtc attactccgc tcatactggg
     1861 actttcggaa gagatccggc agattctgcg gaccgtgatg ctgcccatgc tgctggtcaa
     1921 ctgcgccggc atcgcgctgt tttttttcat ccttcacaac ctcaaccggg agcgcctcaa
     1981 cgaggcggag aagcatcgca tcgacagcga actgcgcatc gcccgcgaaa ttcagatggg
     2041 aatgctgccc gcaccgtctc aaaacggaga tccccgcttt gccgttcacg ccgttttgaa
     2101 gcccgccaaa gaggtgggcg gcgatctgta tcattttttt gcaaaggaca atgaccggat
     2161 ttgtctcgcc gtcggcgatg tgtccgggaa gggcatctcg gcgtctttgc acatggccat
     2221 cacccagaag ctcatcaagg cgctggcgct ctcgggtgag gagcccgcgg agcttctggg
     2281 ccggttgaac cgggagctct gcgacggcaa cgagtcgatg atgttcgtga cgctctttgt
     2341 cgcctttatc catctggaaa gcggaaagac cgtcttcggc aacggcggac acaatcctcc
     2401 ctatcggatc cggcgcggcg atgtttcgcg aatggttctg gagcccggac tggcgctggg
     2461 cattcgggaa aatgcggtct ataccaatca gcggctttgt ctggaggcgg gagacacgct
     2521 ttttctctac accgacgggg tgaccgaagc catgaacggc cgcaaggaac ttttcggcga
     2581 aaaacgtctt gcggcggctc tttcgcgcct gcgggattct gatccggaag ggatctgccg
     2641 ggggctcctg gaggagctcg ctcattttgt tggacaaagc gagcccagtg atgatatcac
     2701 catgctcgcc gtgcggctga cggaaaaatc gaatgcggcg gccggggcgg tccgcggcgg
     2761 ctgagccggc gcaggcgcct tcgcgggaag aggagcggtt gatgaaagta tggtccaaaa
     2821 gacaacagga tgtagtggtt cttgccgttc aggggcgtct cgatgcggcg acggcgcctc
     2881 tttttgaaga gcaggttttg ccgatgattt ccgccgggga caaaaatttt ctgctggatc
     2941 tggccggtct ggactacatc agttcggcgg cgctgcggcg tcttctgttc ttcgcgaaac
     3001 gggcggaaga ccagggcggc agggttgttc ttttatccgt tcagaaccac attctcgaga
     3061 tcctggacat ggccggcttc acccagttgt tcgccatttt cgccgaccag gatgaggccc
     3121 tccgttcctt ttgaggaaaa cgcccatgcc ccggaaaccc tccaatctca tctatggcgt
     3181 cgatgaaaac ccaggcccga tcgcgctcgt cctgatgggg cttcagcaca tctccctgat
     3241 gtccatcgcc tttattttcc ccgtcattat catcgaagcc atcggcggga acctggagga
     3301 agcagagggg ctgattcgaa tggccatgct ggccacgggc atcgggacga tcctgcaggg
     3361 gatcaaccgg gggccggtcg gttcgggcta tctgtgcccg cttctcaacg ggccggcctt
     3421 cctgtccgct tcgctgatgg cgggcaaact gggcggcctt tccctgatct tcggcatgac
     3481 ggcggtcgcc ggtgttttcg aggctttctt ctcccgtttg ctgtcgcgaa tgcgggccgt
     3541 ttttcccgcc gaggtgacgg gcaccattgt gacgatggtg gggatcgaag tcgttcccct
     3601 ttccgtccgg aagtttctgg gaatcgaccc gacgcattcc atgccggatt cgacggctgt
     3661 tctcattgcg atgatcacgc tgggggccat ggcctgcttc aatgtctggg gcaagggcaa
     3721 acttcgcctg tattcggtgc tcctgggaat gatcgtcgga tacgcggcgt ccgccatggc
     3781 ggggcttttg agccccgaca ccttccgggt gatgatggaa gcccctttct ttgcgctgcc
     3841 ggccgtcggc cattacgggt tttcttttga gtgggtcctt ctggttcctt ttctggtcgc
     3901 ggctctctcg tcggctctca aaaccatggg ggatgtgacc acctgccaga aaatcaacga
     3961 tgcccagtgg aagcgccccg acatggtcaa catcagcggc ggcgtcctgg cctgcggcat
     4021 cagcaacatt ctgtccggaa tgctgggcgc tctggggcaa tccgtttcgt cgagcaatat
     4081 cgggctttcc atcgccacgg gcgcaaccag c
//
LOCUS       DAOSIM010000184         4105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_19203_length_4105_cov_7.828642, whole genome shotgun sequence.
ACCESSION   DAOSIM010000184 DAOSIM010000000
VERSION     DAOSIM010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19203_length_4105_cov_7.828642"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="PLR47_08085"
     CDS             complement(<1..1353)
                     /locus_tag="PLR47_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOG12944.1"
                     /translation="MKKEHLRNVAIIAHVDHGKTTLLNAMLKQTGIFRSHQAVEDRVM
                     DSMALEKERGITIAAKNTAVEYQGVKINIVDTPGHADFGGEVERSLNMVDGVLLLVDA
                     SEGPLPQTRFVLKKALARHLPIILVINKIDRPDARVEEVISETYDLFIDLGAEENQIE
                     FPILYTNAKTGVSHAKRGDDSSDLQPLLQAILDAIPAPQGDDGANTQFLVTNLDYDPY
                     VGQLAVGRLQAGKLEMNKPYALLAKEKIISGVKLSALYTFYGLAKKPVTVAESGDIIS
                     LAGVENVTIGDTISSEDRPEALPRLTVDEPTVSMIFYVNDGPFAGREGKYLTSRHLLE
                     RLEKEALRNVAVRIKKLDRTDAFEVCGRGELQMAVLIETMRREGFEMTVSKPQVITRK
                     QDGKTLEPLERLLLDIPEEFVGRITEKLSIRKGRLESLVNKGSGRVSMEFLIPSRGLI
                     G"
     gene            1746..2186
                     /locus_tag="PLR47_08090"
     CDS             1746..2186
                     /locus_tag="PLR47_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOG12945.1"
                     /translation="MTYIKINFGGNFEDEFQKAVDEVFHLVRPAFKNYECIWRPGVDV
                     CESADEVIVLADMAGLNKEELQIEVNRHKLKISGIRKALQLLQSARYRQAEIPHGYFE
                     RSIALPAPVDAQSSVASYADGILMVRMRKLPVNKAHRISVLSAP"
     gene            2220..>4105
                     /gene="lon"
                     /locus_tag="PLR47_08095"
     CDS             2220..>4105
                     /gene="lon"
                     /locus_tag="PLR47_08095"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOG12946.1"
                     /translation="MTEQNVSENKNTFNIPKVIPILPLYNVMVFPKTMIPLEVTGSAS
                     ALVDEAMTKDRLVGLIMARKEPDAAQPYTRDDLHAMGTCAVILKMAKTAENRTQLLLQ
                     GLSRFSIEELVGGKPYQQARIRLVDEKEVKDLETEALMANLLTLFDRILKLSPFLPPE
                     FGPMAKSITEAGTLADLITSVINAPVEEKQKVLDTADVKQRLKELTRMVNHQMEVLEL
                     GNKIQTKVKDDIDKNQREYYLRQQLKAIRQELGESDENTVETDEYRKKIEEKNLPGEA
                     KKEALRELERLSRMHPSSAEYTVSSTYLDWITMLPWHEAATDNLDIAAARKILDEDHY
                     GLEKPKKRIIEYLAVRKLKPDSKGPILCFVGPPGTGKTSLGHSIARALGRKFWRIALG
                     GVRDEAEIRGHRRTYIGALPGRIIQGLRRAESNNPVFMLDEIDKLGSDFRGDPSSALL
                     EVLDPQQNNTFTDHYLDVPFDLSKVLFIATANMLDTIPPALLDRLEVIELTGYTQEEK
                     MKIAERYLIPRQLAENGLSASQFKLTGKALSTIINGYTREAGVRNLEREIANACRGVA
                     AQIAEGSLKSKTVTEKDVHRYLGAVRVPTDIDARITKPGIAIGLAWTPVGGDMLFIEA
                     TAMKG"
BASE COUNT          894 a         1156 c         1196 g          859 t
ORIGIN      
        1 tccgatgagg ccccgcgagg gaatcagaaa ttccatgctg acgcggccgc tgcccttgtt
       61 gacgaggctt tccagtcttc ccttgcgaat ggaaagcttt tccgtgatgc ggcccacgaa
      121 ctcttcgggg atgtcgagca gcagacgctc caagggttca agcgtttttc cgtcctgctt
      181 tcgggtgatg acctgcggct tggagacggt catctcgaat ccctctcgtc gcatggtttc
      241 aatcagtacg gccatctgca gttcgccccg tccgcacact tcaaacgcgt cggtgcggtc
      301 cagttttttg atccggaccg caacgttgcg cagcgcttcc ttttcgagcc tttccagaag
      361 gtggcgggac gtcaggtatt tgccttcccg gccggcaaac ggcccgtcgt tgacgtagaa
      421 gatcatggaa acggtcggtt cgtccaccgt cagccggggc agggcctccg gacggtcctc
      481 cgatgaaatc gtgtcgccga tggtgacgtt ttccacgccg gccagagaga taatgtcgcc
      541 cgactccgca acggtgaccg gctttttggc cagtccgtag aaggtataaa gggcggacag
      601 cttgacgccg cttatgatct tctcttttgc caagagggca tagggcttgt tcatttccag
      661 ttttccggcc tgcaggcggc cgacggccag ttgaccgaca tacggatcat aatccagatt
      721 cgtcactaaa aattgcgtat tggcgccgtc gtctccctga ggcgcgggaa tcgcgtccag
      781 aatggcctgc agcagcggct gcaaatcgga actgtcgtcg ccgcgtttgg cgtggctgac
      841 gccggtcttg gcattggtgt acagaatggg gaactcaatc tgattttctt cggcccccag
      901 atcgatgaag aggtcgtagg tttcggagat gacttcttcc acgcgcgcgt ccggccggtc
      961 gatcttgttg atgacgagaa tgatgggcag atgacgcgcc agggcttttt tcaggacaaa
     1021 gcgggtctgg ggcagagggc cttcgctcgc gtccaccagc agcaggacgc cgtccaccat
     1081 gttgaggctg cgttcgactt cgcctccgaa atcggcatgg ccgggggtgt cgacgatgtt
     1141 gatcttgacg ccctgatatt ccacggccgt gtttttcgcc gcgatggtga tgccgcgttc
     1201 cttttccagc gccatcgagt ccatcacgcg gtcttccacc gcctggtggg agcggaaaat
     1261 cccggtctgc ttgagcatgg cgttgagcag cgtggttttt ccgtgatcga catgggcaat
     1321 gatcgcgaca tttctcaagt gttctttttt catttttctc tttctatccg gtcgtcaatg
     1381 ggaaggtgtt ttcgaactga acgaatcgaa tcgcaaccgc tgaaatgtcc cgcctgactg
     1441 ttttttgggc gagtgagctc tcccatagca aaatatcgcc gattaaacca ctgatttttt
     1501 ttcaggtttc ctctcgtatc cctggcttaa actattccct gcgtttgttg acgcactcac
     1561 cgtgaaaaga acagtgaaaa gcaacgagtg gaaagcgcaa agaggaaaaa ctcatcgctc
     1621 attgctcatc acttttccct atccaccatt caccatccac tattttcccc cgggccttta
     1681 caaatccgct ttgaatcact atacttttta cgcagtcaaa aaatcgcatt tgagaggcac
     1741 aggaaatgac ctacatcaaa atcaattttg gcggtaattt tgaagacgaa ttccagaaag
     1801 cggtggatga agtctttcac ctggttcgcc cggcctttaa aaattatgaa tgcatctggc
     1861 gccccggcgt tgatgtgtgc gaatcggcgg acgaagtgat cgtgctggcc gacatggcgg
     1921 ggctgaacaa ggaagaactg cagatcgaag tcaatcgcca caagctcaaa atatccggca
     1981 ttcgcaaggc gcttcagctt ctgcaaagcg cccgctaccg ccaggcggaa attccgcacg
     2041 ggtatttcga aagaagcatt gccctgccgg cgcccgtgga cgcgcagtcc tccgttgcct
     2101 cttacgccga cggcattctg atggtgcgga tgcgcaagct gccggtcaac aaggcgcacc
     2161 ggatctcggt cctttcggcg ccctagcggc ttttcgccct acgacaacag gaggttacca
     2221 tgacggaaca aaatgtttct gaaaataaaa acacgttcaa tatcccgaaa gtgattccca
     2281 tcctgccgct ttacaacgtg atggtgtttc ccaaaacgat gattccgctg gaggtgacgg
     2341 gcagcgcctc ggcgctggtg gatgaggcca tgaccaaaga ccggctggtg gggctgatca
     2401 tggccagaaa ggagccggat gccgcccagc cgtacacccg ggacgacctg catgcgatgg
     2461 gcacctgcgc ggtgattctg aagatggcca agacggcgga aaaccgaacg cagcttctat
     2521 tgcaggggct cagccgtttt tccatcgaag agcttgtcgg ggggaagccc taccagcagg
     2581 cgcgcattcg gctggtggac gaaaaggaag tcaaggatct ggaaaccgag gcgctgatgg
     2641 cgaatctgct gacgctgttt gaccggattt tgaagctttc gccgtttctg ccgccggaat
     2701 tcgggccgat ggccaagtcc atcaccgaag cgggaacgct ggcggatttg attacctccg
     2761 tcatcaacgc gccggtggag gagaagcaga aggtgctgga tacggccgac gtcaaacagc
     2821 gcctcaaaga gctgacccga atggtgaacc accagatgga agtcttggaa ctgggcaaca
     2881 agattcaaac caaggtgaag gacgacatcg acaagaacca gcgggaatat tatctgcgcc
     2941 agcagctcaa ggccatccgc caggagctgg gggaaagcga tgaaaacacg gtggagacgg
     3001 acgagtaccg caaaaaaata gaagagaaaa acctgcccgg ggaagccaaa aaagaggcgt
     3061 tgcgcgagct ggagaggctt tcgcgcatgc acccgtcctc ggcggagtac acggtttcct
     3121 ccacgtacct cgactggatc accatgctgc cgtggcatga agccgcgacg gacaacctgg
     3181 acatcgccgc cgcgcgcaaa attctggacg aggatcatta cgggcttgaa aagcccaaaa
     3241 agcggatcat tgaatacctg gcggtccgca agctcaagcc ggattccaag ggtccgatcc
     3301 tctgcttcgt ggggcctccg ggcaccggca aaacctcgct ggggcattcc atcgcgcggg
     3361 cgctggggcg caagttctgg cgcatcgcgc tgggcggcgt gcgcgacgaa gcggaaatcc
     3421 gcggccatcg ccgcacctac atcggcgctc tgccgggccg gatcattcag ggcctgaggc
     3481 gcgcggaatc gaacaatccc gtgttcatgc tcgatgagat cgacaagctt ggaagcgact
     3541 tccgcggcga tccctcttcc gcgctcctgg aggtgctgga cccgcagcag aacaacacct
     3601 tcaccgacca ttacctggat gtgccgttcg atttgtcgaa agtgctgttc atcgccacgg
     3661 ccaacatgct tgacacgatt ccgccagcgc ttctggaccg tctggaggtg atcgaactga
     3721 ccggctacac gcaggaagaa aagatgaaga tcgcagagcg gtatctcatt ccgcggcagc
     3781 ttgcggaaaa cggcctttcc gcttcccagt tcaaactgac gggcaaggcg ctttccacga
     3841 tcatcaacgg ctacacgcgg gaagcgggcg tgcgcaacct ggagcgggag atcgccaacg
     3901 cctgccgcgg cgtggcggcg cagattgccg aagggagcct caagtccaaa accgtgacgg
     3961 agaaagacgt gcaccggtat ctgggagcgg tgcgcgtgcc gacggacatc gatgcgcgga
     4021 tcacgaaacc gggcatcgcc atcggtttgg cctggacgcc cgtgggcggc gacatgcttt
     4081 tcatcgaagc caccgccatg aaggg
//
LOCUS       DAOSIM010000185         4077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_19352_length_4077_cov_9.732720, whole genome shotgun sequence.
ACCESSION   DAOSIM010000185 DAOSIM010000000
VERSION     DAOSIM010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19352_length_4077_cov_9.732720"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..2212
                     /locus_tag="PLR47_08100"
     CDS             41..2212
                     /locus_tag="PLR47_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOG12947.1"
                     /translation="MKKMQPTLAAERQYAVEKEKFVPVSQYFGEDTFSHAVIKEKLPK
                     DVFKKLMQAMTENKSLDEETANVVAHAMKEWALEKGATHFAHWFQPMTGITAEKHDAF
                     ADPCGPGAVIERFSGKQLVQGEPDASSFPSGGIRATFEARGYTAWDMSSPAFIKRNGI
                     STTLCIPTVFISYTGQVLDKKTPLLRSLRALNQSALRMLKLLGAKSVKKVQVNLGIEQ
                     EYFLIDMDYYYKRQDLVLAGRTVVGAPPPKGQELEDQYFGSIKERISSFMHDVEEELY
                     KLGIPAKTRHNEVAPSQYEIAPVFEEANLATDHNQMIMETLKHVAKKHRLSALLHEKP
                     FAKINGSGKHVNWSLSDDRGNNLLNPGKTPQDNISFLVFLIAAVRAVYRHGDFLRATV
                     ASCGNDHRLGANEAPPAILSVFLGEQLTQILDNIEKGIVHKATNAEIIDLGISSLSQV
                     SKDYTDRNRTSPFAFTGNKFEFRAVGSSQSVACPAYAINTIVAESLDELAEKIKAKGS
                     KNINQAVFDVLKNEIAQLRPILFNGDNYSKEWAAQAKKLGLPNEKTTPGALKALVTDR
                     ALRLFEKYRVLSNEELRARYLIHTERYIKDLEIEVNCLINICLTQVIPAAAAYQQKLA
                     RAILDAKEVLGSAAVISSQAELLKKILDLINDIYAACREITAGMQAAKAMDGEQKKAE
                     ALCARVKPKMDELREYVDALENIVDDEIWPLPKFWEMLFIC"
     gene            2231..3760
                     /gene="guaA"
                     /locus_tag="PLR47_08105"
     CDS             2231..3760
                     /gene="guaA"
                     /locus_tag="PLR47_08105"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOG12948.1"
                     /translation="MILIIDFGSQYNQLIARRVRENQVYCRIEPPELPLSAIRAMAPE
                     GIILSGGPASIYAAGAPRAQSGIFELGIPVLGICYGLQFMVDALGGRVIGSAKREYGF
                     AELTIKKQKGLFSGVKPKTTCWMSHGDSIGELPRGFSVTASTANTKVAAAEDARRRFY
                     GLQFHPEVVHTREGGKMLANFLFGVCNCARTWSMRSFVAEAVEDIRRQVGKEKVILGL
                     SGGVDSSVAAALLHRAIGKQLTCVFVDNGVLREGEAKRVIDMFKKHLRINLRFVRAGR
                     IFLQRLKGVTDPERKRKIIGRTFIEVFDAEARKLKDVRFLAQGTLYPDLIESRSAFGG
                     PSAVIKSHHNVGGLPKTMKLKLVEPLRHLFKDEVRLLGKELGLADDVVWRQPFPGPGL
                     AIRIIGEVTVPRLSLLKRADRILHQEIRDANLYGKLWQSFTILLPLKSVGIMGDQRTY
                     ENIVAIRAVTSDDAMTADWARLPHDLLARISTRIINEVRGINRVVYDISSKPPGTIEW
                     E"
BASE COUNT          921 a         1170 c         1187 g          799 t
ORIGIN      
        1 gagtaaagag gagcatctct tgcaaaagga tggcaaagcc atgaaaaaga tgcaacccac
       61 gctcgcggcg gaaaggcaat acgcggtcga aaaggaaaag tttgttcccg tatcgcagta
      121 ttttggcgag gacaccttca gccatgcggt cattaaagag aagcttccca aagatgtttt
      181 caaaaaattg atgcaggcga tgactgaaaa caagtcgctg gacgaagaga ccgccaatgt
      241 cgtcgcgcac gccatgaagg agtgggcgct ggaaaagggc gcgacccact ttgcgcactg
      301 gtttcagccc atgacgggca tcaccgccga aaagcacgac gcctttgccg atccctgcgg
      361 ccccggcgcg gtgatcgagc ggttttccgg caagcagctg gtgcaggggg agccggacgc
      421 atcgagtttc ccctccggcg gcattcgggc gacctttgag gcccgcggct acacggcgtg
      481 ggacatgtct tcgcccgcct tcatcaagcg caacggaatc tccacgacgc tttgcattcc
      541 gacggttttt atttcctata cgggccaggt gctggacaag aaaacgccgc ttctgcggtc
      601 gctgcgcgcg ctgaatcaaa gcgcgcttcg catgctgaaa ctgctggggg caaaaagcgt
      661 gaaaaaagtc caggtcaatc tgggcatcga gcaggagtat tttctgatcg acatggacta
      721 ctactacaag cgccaggacc tggtgctggc cggccggacg gtggtcggcg cgccgccgcc
      781 caagggacag gagctggaag accagtattt cggcagcatc aaggaacgga tttcgtcctt
      841 catgcacgac gtggaggaag agctctacaa gcttggcatt ccggccaaga ccaggcacaa
      901 cgaggttgcg cccagccagt atgaaatcgc gcccgtgttt gaggaagcca atctggccac
      961 ggaccacaac cagatgatca tggagacgct gaagcacgtc gcgaaaaagc atcgcctttc
     1021 cgcgcttctg catgaaaagc cgtttgccaa aatcaacggc tccggcaagc atgtaaactg
     1081 gtcgctttcc gatgaccgcg gcaacaatct gctcaacccc ggcaaaacac cgcaggacaa
     1141 tatctctttt ctggtgtttc tcatcgccgc ggtccgcgcc gtttaccggc acggagattt
     1201 tctgcgcgcc acggtcgcat cctgcggcaa tgaccaccgt ctgggcgcca acgaagcgcc
     1261 gccggccatc ctctccgtgt tcctgggcga acagttgacg cagatcctgg acaatatcga
     1321 aaaaggcatc gtgcacaaag cgaccaacgc ggaaatcatc gatctgggca tttcctctct
     1381 gtctcaggtc agcaaggatt acacggaccg caacaggacc tctccttttg cctttaccgg
     1441 caacaagttc gaattccgcg cggtgggctc gtctcagtcc gttgcctgcc ccgcctacgc
     1501 catcaatacg atcgtggcgg aaagcctgga cgaactggcc gaaaagatca aggccaaagg
     1561 atccaagaac atcaaccaag cggtttttga cgtcctgaaa aacgagatcg cgcagcttcg
     1621 gccgattctg ttcaacgggg acaactattc aaaggaatgg gcggcccaag cgaagaagct
     1681 cggcctgccc aatgaaaaaa caacacccgg cgcgctcaaa gccctggtga ccgaccgggc
     1741 gcttcgtctt tttgaaaaat atcgggtgct ttccaacgag gagctgagag cccgctacct
     1801 gatccacacc gagcgctaca tcaaggatct ggaaatcgag gtcaactgcc tcatcaacat
     1861 ctgtctgacg caggtgatcc cggcggccgc ggcctatcag caaaaactgg cgcgggcgat
     1921 tcttgacgcc aaagaagtcc tcgggtccgc cgcggtgatt tcctcccagg cggagctttt
     1981 gaagaaaatt ctggatttga tcaacgacat atacgcggcg tgcagggaaa tcacggccgg
     2041 aatgcaggcc gccaaggcga tggacggcga gcagaaaaaa gcggaggcgc tttgcgccag
     2101 ggtcaaaccg aaaatggacg agcttcgcga atatgtggac gctcttgaaa atatcgtgga
     2161 tgatgaaatc tggccgctcc ccaaattctg ggaaatgctc tttatctgct gagaagacag
     2221 gaaccccctc atgattctga ttattgattt cggctcgcag tacaaccagc tcatcgcgcg
     2281 gcgggttcgc gaaaaccagg tgtattgccg gatcgagccg cccgaacttc ccctttccgc
     2341 cattcgggcg atggcgccgg aaggcatcat tctgtccggc ggcccggcca gcatttacgc
     2401 cgccggcgcg ccgcgcgcac agagcggcat ctttgagctg gggattccgg tgctgggaat
     2461 ctgctacggc ctgcagttca tggtggacgc cctgggcggg cgggtcatcg gctcggccaa
     2521 acgggaatac ggtttcgccg aactcaccat caaaaaacaa aaaggcctct tttccggcgt
     2581 gaagccgaaa acgacctgct ggatgagcca cggcgactcc atcggagaac tgccgcgggg
     2641 cttttccgtc acggcttcca cggccaacac gaaggtcgcg gccgccgagg acgcccgaag
     2701 gcgcttttac ggcctgcaat tccatcccga ggtggttcac acccgcgagg gcgggaaaat
     2761 gctggccaat tttcttttcg gggtctgcaa ctgcgccagg acctggtcga tgcggtcttt
     2821 tgtcgccgag gccgtggagg acattcgccg gcaggtggga aaggaaaagg tcattctcgg
     2881 gctttccggc ggcgtcgatt cgtcggtcgc ggcggcgctc ctgcaccggg cgatcggcaa
     2941 acagctcacc tgcgtttttg tggacaacgg cgttctgcgc gaaggagaag caaagcgcgt
     3001 catcgacatg ttcaagaagc acctgcgcat caacctgcgg tttgtccggg cgggaagaat
     3061 attccttcaa aggctcaaag gggtgacgga tcccgaaagg aagcgcaaga tcatcggccg
     3121 gacgtttatt gaagtgttcg acgcggaagc gcgcaaactg aaagatgtcc ggtttctggc
     3181 gcagggaacg ctgtaccccg atctgattga atcgcggtcc gctttcggcg gccccagcgc
     3241 ggtgatcaag tcgcaccaca atgtcggcgg tttgcccaaa accatgaaat tgaagctggt
     3301 ggagccgctg aggcatctgt tcaaggatga agtgcggctc ctgggaaagg aattggggct
     3361 ggcggacgac gtggtttggc ggcagccttt tcccggaccg ggcctggcca tccgcatcat
     3421 cggggaagtg acggttccgc gcctgtccct tctgaagcgg gcggaccgga tactgcatca
     3481 ggaaatccgc gacgccaatc tctacggcaa attgtggcag tcgtttacga ttttacttcc
     3541 gctgaaaagc gtgggcatta tgggcgacca gcgaacgtat gaaaacatcg tggcgatccg
     3601 ggccgtcacc agcgacgacg ccatgaccgc cgactgggcc cgcctgccgc atgacctgct
     3661 cgcgcgtatt tccacccgca tcatcaacga ggtgcgcggc atcaaccgcg tcgtgtacga
     3721 catcagctcc aagccccccg gcacgatcga gtgggaataa gcgaattaca ttttattccg
     3781 tcatacaact tgttggcaac ttttataatg ggttttcaga aaagttgtca accaatcatt
     3841 gaagagtctg acgccgccgc aggccggtga gaagagggga tcatggcgca cgcatcgcgt
     3901 ctcgccgtga tacccccttt tttttgttat tcgtaaatga tttcgtccgc ggcttccgct
     3961 tccgcggcat ccagttcgca gtcgcgggtc aggcccgccg ccttggctat gaaatattcc
     4021 cggtaccatt catgggcggt gatcatggac atgacctcgc tcgttccggt ccagatg
//
LOCUS       DAOSIM010000186         4046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_19555_length_4046_cov_8.907291, whole genome shotgun sequence.
ACCESSION   DAOSIM010000186 DAOSIM010000000
VERSION     DAOSIM010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19555_length_4046_cov_8.907291"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PLR47_08110"
     CDS             complement(<1..300)
                     /locus_tag="PLR47_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOG12949.1"
                     /translation="MEPIKIMPCLDMKNGRVVKGVHFVDIKEAGDPVENAAFYQAEGA
                     DELAMLDIAATLENRKTRLEWVKKVSAQISIPLTVGGGISSLEDIEMVLGAGASKV"
     gene            486..1451
                     /locus_tag="PLR47_08115"
     CDS             486..1451
                     /locus_tag="PLR47_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOG12950.1"
                     /translation="MKMKEIKASTMNVEKFIDEKVRDIRSAVGDGLAVNALSGGVDSC
                     VVTALGHKALGGRLKTYMIDNGIMRENEPARVQTAFKKLGIHVEVIDASKAFFAALRG
                     LTDPEEKREAITQTFYKNVFGKLVVKSKAKYLLQGTILTDVDETVAGIKRQHNVFEQL
                     GIDPQKAFGYKILEPLIELRKDGVRKAGAGLGLPSAMFDRIPFPGPALAARVIGEVTP
                     ARIALVRKATTITEAALKDTKAFQYLAILHEDRVTGMRDGKRVFGNQIEIRSWESTDA
                     RRASPSRLSFDVLEKIAAKITKEIPEVVSVTYNITTKPTSTIEAI"
     gene            complement(1720..2520)
                     /locus_tag="PLR47_08120"
     CDS             complement(1720..2520)
                     /locus_tag="PLR47_08120"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG12951.1"
                     /translation="MDLGIQGKISVVTGGGKGIGEAIALRLASEGAKVVLADLDSEAA
                     KKAADAIQAQGGNALAVPCNTTNPDQVDALMEKTGETYGAVDILVNNVGGGTGIDLVV
                     RSKVEDWDKTVEMNLKTAYLCCRAAAKVMIPRKRGRIINMSSISGKQGEPLLGSYCAA
                     KFGVIGFTQTLARELARYDITVNAVCPGYVFTPGWEKLAQALKDSQPALADKSLLEIF
                     EARVRSVTPLRRPQEASEIAGLVAYLASYEARSITGQAISIDGGIATG"
     gene            2800..>4046
                     /locus_tag="PLR47_08125"
     CDS             2800..>4046
                     /locus_tag="PLR47_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOG12952.1"
                     /translation="MEKENPKNESAPAKKTPDEKQKKEKAPLRPELAELIKRLSFGLD
                     ENRPDAVEKRKKKNQRTARANIEAFCDPGSFVEYGALAVAAQRGRRSVDDLIAKTPAD
                     GLIAGVAAVNGAHFPEDKARCMIMAYDYTVLAGTQGYFNHKKMDRMLELAYEQKLPLV
                     FFGEGGGGRPGDVDAMTVMVAGLDLGTFGSFARLSGRVPVVGIVSGPCFAGNAALLGC
                     CDVIISTKNSNIGMGGPVMIEGGGLGVFKPEEVGPMDVQTQNGVVDIEVADDIEAVAA
                     AKKYISFFQGPLPAWTAGDPLKLRDVIPESRKRAYNVRSVIKAIADTDSFIELRPRFG
                     PGMVTGLLRIEGRPFGVIANNPMHMAGAIEAEGADKAARLMMLCNAHGLPILSLCDTP
                     GFMVGPEIEKRAQVRHVCRMFVVG"
BASE COUNT          894 a         1208 c         1123 g          821 t
ORIGIN      
        1 cactttgctc gcgcccgcgc ccagcaccat ttcgatgtcc tccagcgagg aaatgccgcc
       61 gccgacggtc agcggaatgc tgatctgggc ggaaaccttc ttcacccatt ccagccttgt
      121 tttgcggttt tcgagtgtgg cggcgatgtc cagcatcgcc agttcatccg cgccttcggc
      181 ctgatagaat gcggcgttct cgaccggatc gcccgcctct ttgatgtcga caaaatgaac
      241 tccttttacc acccttccgt ttttcatatc caggcagggc atgattttga tcggttccat
      301 tccactctcc tttccggggg aagcctttgc gccggctttg cccttcaaac ggcctgcttt
      361 ctaaaccgaa cttcccaaat ttacaatcga aatgtttttg atatttttaa tcttaatgct
      421 tgaaacgcgg ggggttttca tttaaacaaa cctgcaacct attttaccgc aaaggagccg
      481 ccaaaatgaa aatgaaggaa atcaaagcca gcaccatgaa tgtggaaaaa tttatcgacg
      541 aaaaggtccg ggacatccgc agcgccgtgg gcgacggcct cgccgtcaat gcgctctcgg
      601 gcggcgtgga ctcctgcgtc gtcaccgccc tgggacacaa ggcgctgggc ggccgcctca
      661 aaacctacat gatcgacaac ggcatcatgc gggaaaatga gccggcccgg gtgcagacgg
      721 ccttcaagaa actgggcatc catgtggaag tcatcgatgc ctccaaagcc ttctttgccg
      781 ccctcagggg cctcaccgat cccgaagaaa aaagggaagc catcacgcag actttttaca
      841 aaaatgtttt cggcaaactc gtcgtcaaaa gcaaggccaa atatcttctg cagggcacca
      901 ttcttaccga cgtggatgaa accgtcgccg gcatcaagcg ccagcacaac gtctttgaac
      961 agctgggcat cgacccgcaa aaagcgttcg gctataaaat tctggagccg ctcatcgagc
     1021 tgcgcaagga cggagttcgc aaagccggcg cggggctggg cctgccttcc gccatgttcg
     1081 accggattcc cttcccgggg ccggcgctcg ccgcccgcgt gatcggggag gtcacacccg
     1141 ccaggattgc gctggtgaga aaagccacga ccatcaccga agcggcgctc aaagacacga
     1201 aagccttcca gtacctggcg attctgcatg aagaccgcgt cacgggaatg cgcgacggca
     1261 agcgcgtctt cggcaatcag atcgaaatcc gctcctggga aagcacggac gcacgcaggg
     1321 caagcccttc ccggctgtcc tttgacgtac tggaaaagat cgccgcaaaa attacgaaag
     1381 agattccgga agtcgtgagc gtcacctaca acatcacgac caaaccgacc tccaccatcg
     1441 aggccattta gcgctgggga acacggcctt gccgcaaatt tcaaaaaaga aggggcaggt
     1501 ttccctgccc cttcccgtta tcttcaagac ttttcgttcc tatcgtttca gaaagataat
     1561 ggggttgttg gttgccgtgg acagtttttt gacgcgctcg tattctttct gatccccccg
     1621 ccaaacataa agctccgact tgtcgaactt gccgttcgta atagtcccga cggacgccat
     1681 aatccgcaga cggcccgggc ggatgagaag ccgtatggtc taccccgtcg cgatgccgcc
     1741 gtcgatgctg atcgcctggc ctgtgatgct gcgggcttcg taagacgcca gataggccac
     1801 cagcccggcg atttcagagg cctcctgagg acggcgcaga ggggttacgg atcggacacg
     1861 cgcttcaaag atttccagca ggcttttgtc cgccagggcc ggctgcgaat ccttgagcgc
     1921 ctgcgccagc ttttcccagc ccggcgtgaa aacatagccg gggcacacgg cattgaccgt
     1981 aatgtcgtag cgcgccagct ccctggccag ggtttgcgta aatccgatga cgccgaattt
     2041 tgcggcacag tacgaaccca gaagcggctc tccctgtttt cctgaaatgg aggacatatt
     2101 gatgattcgt ccccgttttc tgggaatcat gactttggcg gcggcccggc agcagagata
     2161 ggcggtcttc agattcattt ccaccgtctt gtcccagtcc tcaaccttcg accggaccac
     2221 caggtcaatc cccgtgcctc cgcccacgtt gttgaccaga atatcgacag cgccgtaagt
     2281 ctctccggtt ttctccatga gggcatcgac ctggtccgga ttcgtggtat tgcaggggac
     2341 ggccagcgca ttccctccct gcgcctgaat cgcgtcggca gctttttttg ccgcctccga
     2401 atccagatcc gccaaaacca ccttcgctcc ttcggaagcc aggcgcaaag cgatcgcttc
     2461 gccgatgccc ttgcctccgc ccgtgacaac actgatcttc ccttgaatgc ccaggtccat
     2521 tgtttcgccc ccctgaaatg atttacgtct gaaatacggc aaaaaaatgg ttttgtccat
     2581 gttgatattt taatgacgaa ttcaggctca ccccgcccca tcccgccgcg atgcggctat
     2641 ttccattgac atcaaatttt attttagcta ttcgaaggcg gcaggttaaa aaaaccggtt
     2701 catggtaaga tcgacgtccc ggcgcggggc cggaagaaca accgccgaac cggacggcat
     2761 catgccggaa tcaatagtca atgggggagt ctgaagcgca tggaaaaaga aaacccgaaa
     2821 aacgaatcgg ccccggcaaa aaaaacgccg gatgaaaaac agaaaaagga aaaggcccct
     2881 ttgcggccgg aactggccga actgatcaag cgactttcgt tcggcctgga tgaaaaccgc
     2941 cctgacgcgg tggaaaaaag aaaaaagaaa aatcagcgga ccgcccgcgc caacatcgag
     3001 gccttttgcg atccgggaag ctttgtcgaa tacggagcgc tggccgtggc cgcgcagcgg
     3061 gggcgccgct ccgtggacga cctgatcgcc aaaacgcccg ccgacggtct gatcgccgga
     3121 gtcgccgccg tcaacggagc gcatttcccg gaggacaagg cccgctgcat gatcatggcc
     3181 tacgactaca cggtgcttgc cggaacgcag ggatatttca accacaagaa aatggaccgc
     3241 atgctggaac tggcctatga acaaaagctg ccgctggtct ttttcggcga agggggagga
     3301 ggaagacccg gcgacgtgga cgcgatgacc gtgatggtcg cggggctgga tctgggcact
     3361 ttcggttctt tcgcacggct cagcggacgt gttccggtcg tcggcattgt ttccggcccc
     3421 tgcttcgcgg gaaatgccgc gctgctgggc tgctgcgatg tcatcatctc cacgaagaat
     3481 tccaatatcg gcatgggcgg tccggtgatg atcgaaggcg gcgggctggg ggtcttcaaa
     3541 cccgaggaag tcggcccgat ggacgtgcaa acccaaaacg gcgtagtgga tattgaagtg
     3601 gcggacgaca tcgaagccgt cgccgcggcc aaaaagtata tctctttctt tcagggtccc
     3661 ctccccgcgt ggacggcggg cgatccgctc aaacttcgcg acgtgattcc cgaaagccgc
     3721 aagcgcgcct ataatgtgcg cagcgtcatc aaggcgatcg cggatacgga ttccttcatc
     3781 gaactgcgcc cccgcttcgg ccccggcatg gtcacggggc tgctgcggat tgaagggcgt
     3841 ccattcggcg tgatcgcgaa caaccccatg cacatggcgg gcgccattga agccgagggc
     3901 gcggacaagg ccgcgcgcct catgatgctg tgcaacgcgc acggactgcc gattctgtcg
     3961 ctgtgcgaca cgccgggctt catggttggt cccgaaattg aaaaacgggc gcaggtccgc
     4021 catgtctgcc ggatgtttgt cgtcgg
//
LOCUS       DAOSIM010000187         4011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_19789_length_4011_cov_8.042214, whole genome shotgun sequence.
ACCESSION   DAOSIM010000187 DAOSIM010000000
VERSION     DAOSIM010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19789_length_4011_cov_8.042214"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PLR47_08130"
     CDS             <1..519
                     /locus_tag="PLR47_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOG12953.1"
                     /translation="STAALAMGDALAIALIDKRKFQEKDFYKFHPGGSLGARLRATVR
                     DAMITGDRIPRVLSGAAARKAIEVIDRMNVGFVLVTDARDQLLGILTDGDVRRIVSRG
                     STFEGMTIDQVMTSNPKTIDEKASLAETVEFMQKKEITSLAVVNGKKKLKGYVHLHDI
                     FGRGGSVNISLP"
     gene            540..1073
                     /locus_tag="PLR47_08135"
     CDS             540..1073
                     /locus_tag="PLR47_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOG12954.1"
                     /translation="MALFEFEGKKPQVPSDSFVHPAAVLIGDVRLGHECLIAPGVSIR
                     ADFGPVIIGDRSSVQDNSVIHVYPGSESVIGNDVTVAHGAILHDVHIHSRCMIGIGAI
                     ILFNAVCEEDVFVSVGSLVPRGMRVPAGKIVAGNPAQIKRDATEKDKAAARDGIEAYR
                     YLCRQYLATMKPVTGPA"
     gene            complement(1255..2022)
                     /locus_tag="PLR47_08140"
     CDS             complement(1255..2022)
                     /locus_tag="PLR47_08140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG12955.1"
                     /translation="MASKNENRFDDFFENEKYVSLKNHLYNYLLRKRAVEEAMSSEEK
                     GLVLEIGSGISPVFTSWDRVVYSDVSASALKMLQKIHGRGQYVVADAMNLPFEDGLFS
                     HVIASEVLEHLEDDRRALAEIARVIRPGGILIVTFPHRHFYFAHDDRFVRHCRRYELS
                     EMISRLEEAGFYAVEVQKVLGPLEKLTMLAVTGFASVAQKFQRKERASKDNVPPPKFL
                     IRLFQVMNRFYAVLARFDAWIMPRSLSTVLLIRAKKL"
     gene            complement(2032..2775)
                     /locus_tag="PLR47_08145"
     CDS             complement(2032..2775)
                     /locus_tag="PLR47_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG12956.1"
                     /translation="MFKGKRVVVVMPAYNAAQTLKMTYNEVMAQEMVDLVILVDDASS
                     DQTSIIARGLPKTRVHVHEKNSGYGANQKTCYRMALEEGADIVIMVHPDYQYTPKLIP
                     AMASMIGNGLYHCVLGSRILGGHAVKDGMPIWKYIANRFLTLAENMMLGAKLSEYHTG
                     YRAFSRELLEKLPLERNSDDFVFDNQMLAQIIWQGYTIAEVSCPTKYFTEASSINFRR
                     SVQYGIGCLATAVSFRLSKAGLASSPLFS"
     gene            complement(3230..3316)
                     /locus_tag="PLR47_08150"
                     /pseudo
     CDS             complement(3230..3316)
                     /locus_tag="PLR47_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003112842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            complement(3537..3806)
                     /locus_tag="PLR47_08155"
     CDS             complement(3537..3806)
                     /locus_tag="PLR47_08155"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOG12957.1"
                     /translation="MDLPVDFWNADKLYHCAAYGWLALLPAIGFADRRAALSAAFSMI
                     ILGLLLEAGQYFIPGRMFSVLDILANTLGVMLGILPGRFIGKRLE"
     gene            complement(3790..>4011)
                     /locus_tag="PLR47_08160"
     CDS             complement(3790..>4011)
                     /locus_tag="PLR47_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12958.1"
                     /translation="LHTVEVAGSIPAPPTIQKSGIILSRLIPFLFETDENKTAGKIRQ
                     MVSANPFSLGSLDWHGHFSIAFAPGGSAG"
BASE COUNT         1028 a         1068 c         1018 g          897 t
ORIGIN      
        1 tccaccgccg cgctggccat gggagacgcg ctggccatcg cgctcatcga caaacgcaaa
       61 tttcaggaaa aggattttta caagttccat cccggagggt ccctgggagc aaggctccgg
      121 gcgaccgtcc gggacgcaat gattacgggg gatcgaatcc cgcgcgttct ctccggagca
      181 gccgcgcgaa aggcgatcga agtgatcgac cggatgaatg tcggattcgt gctggtgacg
      241 gatgccaggg accaactgct tggaattctg accgacggcg acgtgcgccg gatcgtcagc
      301 cggggatcca cctttgaagg gatgacgatt gaccaggtca tgacctccaa tccgaaaacc
      361 atcgatgaaa aagcttcgct ggcggaaacc gtcgagttca tgcagaaaaa ggaaatcact
      421 tccctggccg tcgtcaacgg aaagaaaaaa ttaaaagggt atgtccacct gcacgacatc
      481 ttcggccggg gcggctcggt caacatttct ttaccctgac aaaacgaaag gggatggcga
      541 tggcgctgtt cgaatttgaa ggcaaaaagc cgcaggttcc gtccgattct tttgttcacc
      601 ccgcggccgt tttaatcggc gacgtccgcc tcgggcacga atgcctgatc gcaccgggcg
      661 tttccatccg cgcggacttc ggcccggtca tcatcggcga ccgctccagc gtgcaggaca
      721 attccgtcat tcacgtctat cccggctccg agtccgtcat cggcaacgac gtcaccgtgg
      781 cgcacggcgc cattctgcac gacgtgcata tccattcccg atgcatgatc ggcatcggcg
      841 ccatcatcct tttcaacgcc gtctgcgagg aagatgtttt tgtttccgtg ggatcgctgg
      901 ttccaagagg catgcgcgtg cccgccggca aaatcgtcgc cggcaatccc gcccagatca
      961 agcgggacgc aacggagaag gacaaagcag cagccagaga cggcatcgaa gcctaccgct
     1021 acctctgcag gcaatacctg gccaccatga aaccggtcac gggccccgcc taaattaggg
     1081 acaccgccct atttccattt ttttatgcag ggcgagtctt cagactcgcc aaaccctcgt
     1141 gcaaaatcaa cttggcttgg cgcacctgaa ggtacgccct acaaacagac gtcaattact
     1201 taacccttaa aacttaaatc ttaaagctca gaaataccga ggcgctgtcc ctaattataa
     1261 ttttttggcc cggatcagca ggacagtcga taacgagcgg ggcatgatcc aggcgtcgaa
     1321 gcgggccaaa accgcgtaaa accgattcat cacctgaaag agacggatca ggaatttcgg
     1381 gggcggaacg ttatctttgg aagcacgctc tttccgttgg aatttctgcg ccacggaggc
     1441 gaagccggta accgccagca ttgtcagttt ttccagaggc ccaagcacct tttgaacttc
     1501 cacggcgtaa aatccggctt cctccaaccg agaaatcatc tcggacagct cgtagcggcg
     1561 gcaatgacga acgaaacgat cgtcatgcgc aaagtaaaaa tgacgatggg gaaacgtcac
     1621 aatcaaaatc ccgccaggcc tgataactct agcgatttcc gctagcgccc tacgatcgtc
     1681 ttccagatgc tccagaacct ccgaggcgat cacatgggaa aacaatccgt cctcaaaagg
     1741 cagattcatc gcgtccgcga caacgtattg cccccggcca tgtatttttt gcagcatttt
     1801 cagggcggat gcggacacat cagaatagac cacccgatcc caggaagtaa agaccggcga
     1861 gatgccgctg ccgatctcca ggaccagacc tttttcttcc gagctcatcg cctcttcaac
     1921 ggcgcgtttt cgcaagaggt aattgtacaa gtgatttttc agggaaacat atttctcgtt
     1981 ttcaaaaaaa tcatcgaagc ggttttcgtt tttggatgcc atgaccggtt ctcaagaaaa
     2041 aagcggtgaa ctggccagac ccgctttgga caggcggaaa ctcacggccg tcgccagaca
     2101 gccgatgccg tattggacgc tgcgcctgaa gttgatggac gaggcttcgg taaagtattt
     2161 ggtcggacaa ctgacctcgg caatggtata gccctgccag ataatctggg ccagcatctg
     2221 attgtcaaaa acaaaatcgt cggaattccg ctccagcggc aatttctcca ggagttcccg
     2281 tgaaaaggcg cggtagcccg tgtgatactc ggagagcttc gcgcccagca tcatattttc
     2341 cgcaagcgtg agaaatcgat tggcgatgta cttccagatc ggcatcccgt ccttcaccgc
     2401 atggcctccc agaattctgg agccgaggac acagtgataa agtccgttgc cgatcatgga
     2461 tgccatggcc gggatcagct tcggcgtgta ttgataatcg gggtgaacca tgatgacgat
     2521 gtccgcgccc tcctccagag ccatccgata gcaggttttc tgattggcgc cgtatccgct
     2581 gttcttttcg tggacatgaa ccctcgtttt gggcagtccg cgggcaataa tggatgtttg
     2641 atcggaactg gcatcgtcca ccagaatcac caggtccacc atctcctgtg ccatcacctc
     2701 attgtaggtc attttcagcg tctgcgccgc gttgtacgcg ggcatgacca caacaactct
     2761 ttttccttta aacattcccc gaatctccgc aatcgcgttg atattgatca gtcagctcac
     2821 cctccagaaa ggataacgct ggaactttat acatgatgtc ggcgacaaat gaaatatgaa
     2881 tgatggaacg tttttcagtt ggaaagaaaa gggcttcatg gaaaagcccg aaggatcgtg
     2941 aaataatctt gttttaagga gtagcagtaa cagtaaccgt gccgttcggg tcaatggtat
     3001 aggtaacatt gcttgcgtta tgctttgccg gtgccgtcca ggtatcgagc ttatcatccg
     3061 taatgttgag cgtgacgtcg ctggaattga aaaatttctg actatgcatg ccgtcaatcc
     3121 cgcaggtcgt cgaaccttcc attgtggaaa agtaaacctg gcaggccgta taagcgcttc
     3181 ttagttcagc ggttgctttg gtgttgtacg acttgactct gtaagcggaa aattgcggga
     3241 tggcgattgc cgccagaatg ccgatgatcg ccacaacaat catcaactcg atgagggtga
     3301 aacctttttt attcattttt catccccttg tctgaatttt catattgttt cagcaactaa
     3361 tataccaaag gcaaatgcga cttacgcttt ttttgtatgg ggattaaatc aaaaattgca
     3421 aataaattca agaacaaaga aagatcgaaa aaagccgctc cacaaaaggc gcaaaaacct
     3481 ttttacacaa aaaggtgatt ttcacccgaa caagctattt tgaccgtaaa tcattgtcat
     3541 tccagccgtt ttccgatgaa gcgccccgga agaatgccca gcatcactcc cagcgtgttg
     3601 gcaaggatat caagaactga aaacattctt ccgggaataa aatactgccc ggcttccagg
     3661 agcaacccaa gaatgatcat ggaaaaagcg gcggacaaag ccgcgcggcg atcggcaaaa
     3721 ccgatggcag gcagaagcgc cagccatccg tacgcggcgc aatgatagag tttgtccgcg
     3781 ttccagaaat caaccggcag atccacccgg ggcaaaagcg atagaaaaat gaccgtgcca
     3841 atcgaggctc cccagagaaa aaggattggc cgagaccatt tgcctgattt tgccggctgt
     3901 tttgttttca tcagtttcaa aaagaaaagg gattaaccgt gaaaggataa tccctgattt
     3961 ttgaatggta ggcggtgctg ggattgaacc agcgacttct accgtgtgaa g
//
LOCUS       DAOSIM010000188         3975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_20031_length_3975_cov_8.836224, whole genome shotgun sequence.
ACCESSION   DAOSIM010000188 DAOSIM010000000
VERSION     DAOSIM010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20031_length_3975_cov_8.836224"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PLR47_08165"
     CDS             complement(<1..444)
                     /locus_tag="PLR47_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOG12959.1"
                     /translation="MDYKNIIVTTENFVTTIVLNRPPMNSVNLGIREEFFDAVTNIEK
                     SRETRAVIVTGAGEKGFSAGMDVSDVANITRGPNGNDIMNAISRSAKPYIAAINGHAL
                     GGGCETAIACHFRIMTDNPKALIGLPEVNLGITPGWGGIQRLPRLL"
     gene            complement(552..1433)
                     /locus_tag="PLR47_08170"
     CDS             complement(552..1433)
                     /locus_tag="PLR47_08170"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOG12960.1"
                     /translation="MKIDLRQIQSLGSGLTRPEGVMALDDGGVRTADAHGRCARIDPD
                     GRTTFFGQLGGLPNGICIDTDGNCIVANIGNGEVQSLSPDGKHAVLMTEAQGKKMSTP
                     NFPFLDFAGRLWVSNSTDNPDIDASLKSPVPDGCLVLIANGRTPRIVAEGICFANGVA
                     VDADEQYVYVAETMKRRILRYRIHADDTVGPAEVYGPEFLGKLGFPDGIAFDEAGNLW
                     IAFPVANAVGCIDPRGGLEICADDPQGEVLNRPANICFGGKDRRTAFIGSLGGANVPF
                     FEVPHSGQRLVHQKSRP"
     gene            1675..2427
                     /locus_tag="PLR47_08175"
     CDS             1675..2427
                     /locus_tag="PLR47_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG12961.1"
                     /translation="MFNRIVSVVFLVFIALTSIPLFLTALLLRLATYPFDRRLRLLHL
                     FTCFWASLYTWTMPAWRIYIEGREKIRKNATYMVVSNHQSQLDILVAFRLFFHYKWVS
                     KIEIFRVPLIGWNMRLNRYVQLKRGDKQSIEKMLRDCAVHLEEGSSIFMFPEGTRSPD
                     GEVKAFKPGAFQLALQKNVPILPIVISGTNKALPKYSMNFNGVQKIYVKVLEEIPYEA
                     FAHLSVAQTAEMVHGFITRQLRLMKECTPDPA"
     gene            complement(2452..3573)
                     /locus_tag="PLR47_08180"
     CDS             complement(2452..3573)
                     /locus_tag="PLR47_08180"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG12962.1"
                     /translation="MKQPDGLMKAQSQAAEFAARHVAARADLQTTQEFPLDLWREMGE
                     AGLFKIGIPEIHGGTGGGHSDLLKAGEVFVRNGLNLGLGLSWIFQQVIARFVVEEFGT
                     PGQRRQYLPAAALGNCTMSFAVSEPKRGASPKTLATQAEKTGTGYELTGEKTYLTNGP
                     IADLFVVVAATDDAAQRRRFTAFIVPRGTQGLTVTPPMPLNLLKPSPHGGIQLNRCPV
                     PKASVLGREGNAWPDIVVPLGETEDVVMMGPVLGGMAAQLDLLRIALRERSASAEKTL
                     PGEWGALNSLWQALRSIACEAAGRLDRGNESPAPLSIAFARFAALFHADVARFTERLR
                     IEEPERFSLLQKDMEFMEMLQKRRLQIRQAKMGAAQLAS"
     gene            complement(3570..>3975)
                     /locus_tag="PLR47_08185"
     CDS             complement(3570..>3975)
                     /locus_tag="PLR47_08185"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG12963.1"
                     /translation="VFIDQLTNVTGIFEEQDISEAIRVVMEEADVHYSAPTDYGETLR
                     NFNKDYMDLLTKKTTLIIVGDGRSNYMNPEDAILGAMRERCRRVIWLNPEPENLWGTG
                     DSEIQTYLRHCHEVRACRNVNQLFTFIEELVI"
BASE COUNT          765 a         1157 c         1146 g          907 t
ORIGIN      
        1 cagaagcctg ggcagccgct gaatgccgcc ccagccgggc gtgatgccca gattgacttc
       61 cggcagtccg atgagcgcct tggggttgtc cgtcatgatg cggaaatggc aggcgatcgc
      121 cgtttcgcaa ccgccgccca gggcgtgccc gttgattgcc gcgatgtagg gtttggcgga
      181 gcgcgagatg gcgttcatga tgtcgttgcc gttgggtccg cgggtgatgt tggccacgtc
      241 ggagacatcc attccggcgg agaagccttt ttcacccgcg ccggtgacga tgacagcccg
      301 cgtttccctg ctcttttcaa tgttcgtgac cgcgtcgaaa aattcctcgc gaatgcccag
      361 attcacggag ttcatcggcg ggcgattcaa aacaatcgtc gtcacgaaat tttcggttgt
      421 cacaataatg ttcttgtaat ccatctcttt tctcctttct aatggtttta tggttgattt
      481 tgagtctttt gatggccatt cgccctgtcg ctgacccgta caccttcagc cgatgaccta
      541 tagcccatcg cctatggcct gcttttctga tgcaccagcc gctggcccga atgcggcact
      601 tcaaaaaagg gaacgttcgc gccgccaagg cttccgataa aagccgtgcg gcggtccttc
      661 ccgccgaagc agatattggc cggacggttc agcacctcgc cttgcggatc gtccgcgcaa
      721 atctccaggc cgccccgcgg atcaatgcaa ccgacggcat tggcgacagg aaaggcaatc
      781 cagaggttgc cggcttcgtc aaacgcgatg ccgtcgggaa agccgagctt gcccaggaat
      841 tcaggaccgt aaacctctgc cggtccgacc gtgtcgtcgg catgaatcct gtagcgcaga
      901 atgcggcgtt tcatggtttc cgccacataa acatattgtt cgtccgcatc caccgcgacg
      961 ccgttggcaa aacaaatgcc ttcggcgact attcggggcg tccggccgtt cgcgatcaac
     1021 accaggcatc cgtcgggaac gggcgactta agtgatgcgt cgatatccgg gttgtccgta
     1081 gagttggaaa cccagaggcg gcccgcaaaa tccagaaagg ggaaattggg cgtggacatt
     1141 ttcttgccct gcgcttcggt catcaacacg gcatgctttc cgtccgggga cagagactgg
     1201 acttcgccgt tgccgatgtt ggcgacaatg cagttgccgt ccgtgtcgat gcagatgccg
     1261 ttgggcagac cgcccaattg gccgaaaaaa gtcgtccggc cgtccggatc aatgcgggcg
     1321 cagcgtccgt gggcgtccgc cgtgcggacg ccgccgtcat ccagcgccat gacgccttcg
     1381 gggcgggtca gtccgctccc gagggattga atctgccgaa gatcgatttt catgactctc
     1441 ctgctgtttg tcgcgtatgt cgggacccta tcacaatttg atggtcgcgg gcaatcatta
     1501 gtcgccggtt cgggctgttc tttttcgccg ggaaaaaacc gccggctgcg aaacctgtcg
     1561 ccggcgccgc gaaggaatgc gcctgcatga aggaatgtga ttgacaggtt cgaatcttct
     1621 gtctatactc cgcgccatcc tgacaggaaa taatcgcaag taaacggatt ttccatgttc
     1681 aatcgcatcg tcagcgttgt ttttctggtc tttatcgccc tgacatccat cccgttgttt
     1741 ttgacggctc tgctcctgcg tctggcgacc tacccctttg accggcgttt gcggcttttg
     1801 cacttattca cgtgtttctg ggcttcattg tacacctgga caatgccggc ctggcggatt
     1861 tacatcgaag gccgcgaaaa aatccggaaa aacgcgacgt acatggttgt atccaatcat
     1921 cagtcgcagc tcgatatcct ggttgccttc cggcttttct ttcactacaa gtgggtgtcc
     1981 aagattgaaa tattccgtgt gccgctgatc ggctggaata tgcggctgaa ccgctatgtt
     2041 cagctcaagc gcggcgacaa gcagagcatc gaaaaaatgc tgagggattg cgcggtgcac
     2101 ctggaagagg gaagttccat tttcatgttt ccggaaggca cacggtcgcc cgacggcgag
     2161 gtcaaggcct tcaaacccgg cgctttccag cttgcgctcc aaaagaatgt gcccattctg
     2221 ccgattgtca tcagcgggac caataaggct cttcccaaat acagcatgaa ttttaacggt
     2281 gtgcagaaaa tttacgtcaa ggtattggaa gaaattccgt atgaggcctt cgcgcatctt
     2341 tcggtcgccc aaactgctga aatggttcac ggttttatca cccgtcaact gcggctgatg
     2401 aaggaatgca cgccggaccc ggcctgatcg cttttttcac gaaaagcccg ctcaggaagc
     2461 aagctgtgcc gcgcccattt ttgcctggcg gatctgcagg cgtctttttt gaagcatttc
     2521 cataaattcc atgtcttttt gaagaaggct gaagcgttcg ggctcttcga tgcgcagacg
     2581 ttcggtgaac cgggcgacgt cggcgtgaaa caacgccgcg aagcgggcaa aggcgatgct
     2641 tagaggcgcg ggcgattcat tgccccggtc cagcctgccg gctgcttcgc aggcgatgct
     2701 tcgcagggcc tgccacaggg aattcaacgc gccccattca cccggcagag ttttctccgc
     2761 ggatgcggag cgctcccgaa gggcgattct cagaagatcc agctgcgcgg ccattccgcc
     2821 cagaacgggt cccatcatca ccacgtcttc cgtttctccc agggggacga cgatgtccgg
     2881 ccaggcgttt ccttcccggc cgaggacgga tgccttcggg accgggcagc ggttcagttg
     2941 aatgccgcca tgcggcgaag gctttaacaa attcaagggc atcggcgggg tgacggtgag
     3001 gccttgcgtg ccgcgcggaa cgatgaacgc ggtaaatctt ctgcgctgtg ccgcgtcgtc
     3061 cgttgcggcg acgaccacaa acaggtctgc aatcggtccg ttggtcaggt aggttttttc
     3121 gcctgtcagc tcataccccg tcccggtttt ttcagcctgc gtggccagcg ttttagggga
     3181 agcgccccgt tttggctcgg aaacggcaaa gctcatcgtg cagttgccca gggcggcggc
     3241 gggaagatat tgccggcgct gccccggcgt gccgaattct tccacgacaa agcgggcgat
     3301 cacctgctgg aagatccagg ataagccgag tcccagattg aggccgtttc gaacaaaaac
     3361 ctccccggct ttcaagaggt cggaatgccc gccgcccgtc ccgccgtgaa tttccggaat
     3421 gccgattttg aaaagtccgg cctcgcccat ctcccgccac agatccaggg gaaattcctg
     3481 cgtggtctgc aaatccgcgc gggccgcgac gtgccgggcg gcaaactccg ccgcctgcga
     3541 ctgcgctttc atgaggccgt ccggctgttt catatgacca gctcctcgat gaacgtgaac
     3601 aactggttga cgtttcggca ggcgcggact tcgtgacagt ggcgcaggta ggtctggatc
     3661 tcgctgtcgc ccgtgcccca caggttttcc ggttcggggt tgagccagat gacccggcgg
     3721 catcgttccc gcatggcgcc cagaatcgcg tcttccggat tcatgtaatt cgaccgcccg
     3781 tcgccgacga tgatgagcgt cgttttcttc gtcagcaggt ccatgtagtc tttgttgaaa
     3841 tttctgagcg tctcgccgta atcggtcggc gccgagtagt gcacgtccgc ttcttccatg
     3901 acgacgcgga tggcttcgga aatatcctgc tcctcaaaga tgccggtgac gttcgtcaac
     3961 tggtcgatga agaca
//
LOCUS       DAOSIM010000189         3951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_20174_length_3951_cov_7.237166, whole genome shotgun sequence.
ACCESSION   DAOSIM010000189 DAOSIM010000000
VERSION     DAOSIM010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20174_length_3951_cov_7.237166"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="PLR47_08190"
     CDS             <1..1042
                     /locus_tag="PLR47_08190"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG12964.1"
                     /translation="VLMYQPISQIPKIMAGVNVSKTDLEIAKTDQARATLQVKQTAEK
                     LYFGLLIQEKRREEAQIRLDLAQKKLYDVESAVLAGKTTPSSLTGLRANAADEEQNLL
                     KINVQMDDYFDDLRRLTGLKSASFALAPVPVDDADPSAATNGVSGRQENQDLKIAALY
                     QTKAQHAVDAGHYSYLPSLGILGGYVYQHGAADFTGDIRYSFISAGFGLDPQWQKRDA
                     FIGVVLNWNLQDLVANTYVTRQRIFLKKQAEENLANTREQVHADTEKARRKLTQAAQL
                     IGVAGKVVAYRREDFKIQTDRFSAGLNLEADWLAAKAALAKSEADLLAARLSYRMAWT
                     DLQILTGRYGGE"
     gene            complement(1027..1731)
                     /locus_tag="PLR47_08195"
     CDS             complement(1027..1731)
                     /locus_tag="PLR47_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOG12965.1"
                     /translation="MADEIKTNILNICERIAAAAIRAGRDPAGIRLMAVSKTVEPERI
                     RQALDAGITLLGENYVQEAREKIPVVGRPAVWHMIGHLQTNKVKYVVNLFDWIHSVDR
                     LELARELDKRAGQRSRRLNVLIEVNVSGEASKNGAAPGQVLELVRQISILPNVSVRGL
                     MTMPPYSDDPENSRPCFIVLRQLRDEIVSAALPGISMDELSMGMTDDFEVAIEEGATI
                     IRVGRAIFGERRYSPP"
     gene            complement(1737..2336)
                     /locus_tag="PLR47_08200"
     CDS             complement(1737..2336)
                     /locus_tag="PLR47_08200"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOG12966.1"
                     /translation="MIMVLSLSSPLILASASPRRRELLRSVGLTFKIRPADVDETGLP
                     DESPRAHVRRLSRDKAGAIAHQYPKALVLGADTIVVIDGRILGKPKNKKEAREMLQRL
                     SSRRHTVLTGFTIACVRAGMSRTKIVQSTVQFKKISPEETAWYVNGDEPYDKAGGYAA
                     QGKGASFIQAIRGSYTNVIGLPLCEVVEELNHLGVLHFR"
     gene            2791..2919
                     /locus_tag="PLR47_08205"
     CDS             2791..2919
                     /locus_tag="PLR47_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12967.1"
                     /translation="MIAFDDEKTNLDVIMKALRAGDFAVQDKPVYLKAFPAETQSN"
     gene            complement(2907..>3951)
                     /locus_tag="PLR47_08210"
     CDS             complement(2907..>3951)
                     /locus_tag="PLR47_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOG12968.1"
                     /translation="TRFWPRSREKKPKHLLDIVSDRTIIQETVDRIKPLIKPKNILIV
                     TGKKHARELMRQLPEVPAKNIIIEPVGRNTAACIGLAALHIQKKVKDDVMVVLPSDHG
                     IADPARYRSVIAAAARAAEKKSALVTIGLTPASPHTGFGYMEGSASAGNVAGEKLLRV
                     KAFREKPNLEQARIFLQNGNFFWNSGMFIWNASTILKEIEKFLPDLHSGLMTIKSSLG
                     TSSEARTVSKIYRSLASVSIDYGVMEKAKDVFMIPAGFGWSDVGSWDTLWEISQKDFK
                     GNALAGGSQAMYENAENTLVYSPNKLVALIGIKDILVVETKDALLVCQRGQSQDVKKI
                     VDALEAEGKRQLL"
BASE COUNT          846 a         1072 c         1121 g          912 t
ORIGIN      
        1 agtgctgatg tatcagccca tttcgcagat tccgaaaatc atggccgggg tcaatgtgtc
       61 caagacggat ctggaaatcg ccaaaaccga tcaggcaaga gcgacgctgc aggtgaagca
      121 gaccgccgaa aaactctatt tcggcctgct gattcaggag aagcgcaggg aagaggccca
      181 aatccgactg gatctggccc aaaaaaaact ctacgacgtg gaaagcgccg tgctggccgg
      241 caagacgact ccctccagcc tgaccggtct cagggccaat gccgccgacg aagagcaaaa
      301 ccttctcaag atcaacgtcc agatggacga ttattttgac gatttaaggc ggctgaccgg
      361 tttaaaatcc gcctcctttg cacttgcgcc cgtgcccgtg gacgacgcgg acccgagcgc
      421 cgcaacaaac ggtgtttccg ggcggcagga gaaccaggat ttgaaaatcg ccgcgctgta
      481 tcagacgaag gcgcagcacg cggtggatgc cggacactac agctacctgc cgagtttagg
      541 cattctgggc ggctacgttt accagcacgg cgcagccgat tttaccggtg atatccggta
      601 ttcgtttatc agcgccggct tcggtctgga tccccagtgg caaaagcggg atgcgtttat
      661 cggcgtggtt ttgaactgga atctccagga cctggtcgcc aacacctacg ttacccgcca
      721 gcgcattttt ctgaaaaagc aggccgagga gaatctggcc aataccaggg aacaggtcca
      781 tgccgacacg gaaaaagcga ggcgcaaact gacgcaggcc gctcaattga tcggcgttgc
      841 cgggaaagtc gttgcatacc gccgcgaaga tttcaaaatt caaaccgacc ggttttccgc
      901 cggactgaac ctggaagcgg actggctggc ggcaaaggcc gccctggcga aatccgaagc
      961 ggatctgctg gccgcgcggc tgagctaccg catggcctgg accgaccttc aaatcctaac
     1021 cggacgctat ggaggcgaat agcgtctttc cccgaaaatc gcgcggccga cgcggataat
     1081 ggtggcgcct tcttcaatgg ccacttcaaa atcatccgtc atgcccatgg agagttcgtc
     1141 cattgaaatg cctggaaggg cggcggaaac gatttcatcg cgcagttgac gaagcacaat
     1201 aaaacaaggg cgcgaatttt ccggatcatc ggaatagggc ggcatcgtca tcaaacccct
     1261 cacgcttaca ttcggtaaga tggaaatctg acggaccagt tccaaaactt gcccgggcgc
     1321 tgcgccgttt ttcgatgcct cgccgctgac attgacctcg atgagcacat tcaaccgtcg
     1381 gctgcgctgg ccggctcttt tatccaactc ccgcgccagt tccaaacggt ccaccgaatg
     1441 aatccaatca aagaggttga cgacatactt gaccttgttg gtttgcaggt ggccgatcat
     1501 gtgccatacg gcaggccgtc cgacgacggg gattttctcg cgggcctcct gaacgtagtt
     1561 ttcacccaaa agcgtgattc ccgcatccag ggcctgccgg atgcgttccg gttccacggt
     1621 tttggagacc gccatcagac gaatgcccgc cgggtcgcgg cccgcgcgga ttgcggccgc
     1681 tgcgattctt tcgcaaatgt tcaaaatatt ggtcttgatt tcatcggcca tggtctttac
     1741 ctgaaatgaa ggacacccaa atggttcaat tcttccacga cttcacaaag cggcaacccg
     1801 atcacattgg tataagagcc ccggatggcc tgaatgaagg acgcgccctt gccctgagcc
     1861 gcatagccgc cggctttgtc atagggctcg tcgccattga cgtaccatgc cgtttcttca
     1921 gggctgattt ttttaaactg cacggtggat tggacgattt ttgttctgga cattcccgca
     1981 cggacgcagg cgatcgtaaa accggtcaaa acggtgtgtc gccggctgct cagccgctgc
     2041 agcatttcac gggcctcttt tttgtttttg ggttttccca gaatccggcc gtcgatgacc
     2101 acaatggtgt ccgcgcccag caccagggct ttcgggtatt gatgggcgat cgcgcctgct
     2161 ttatctctgg acagccggcg aacgtgagcg cgcgggcttt catccggaag gccggtttca
     2221 tccacgtccg ccggacggat tttgaacgtc aaaccgaccg aacggagcag ttctctccgg
     2281 cgtggtgagg cggaggccag gatcagggga gacgacaggg acagaaccat aatcatttct
     2341 ttctttttta aagtcgggct ttttattcca gaataatcat ggcctgtcaa ttcgaaattg
     2401 gcattgcgtt tttcggaagc gtggtttagt ggaagctcat tggtggatgg tgaatcgtgg
     2461 atggtgaatg gtggactgta gggaacggct tgtgaccttt aggttatcgc gaccgttcca
     2521 tcgaaaaaca gggacagcgc ccatttactg tagggcgggc cttcaggccc gcagtgataa
     2581 atcatggatg gtgtgatgag aaaaatgaag aggctggttg cgatatttct gtttctggtg
     2641 ctgtcggtcg gtgcggccgg tgcggcggag agaatcgtgc agttgaaggt gcccggatgt
     2701 ttttcgtgag gcgccgaccg aaggataggg actatcctga agaaaattga cggggttcaa
     2761 aaacatcaga cgcaaagacc gaacagcctg gtgattgcct ttgacgatga aaagaccaac
     2821 ctggacgtga ttatgaaagc cctgcgggca ggcgattttg ccgtacagga caagccggtt
     2881 tatctgaaag cttttcccgc cgaaactcaa agcaactgac gctttccctc tgcctccagc
     2941 gcgtccacga ttttcttgac gtcctgcgac tgccctcttt ggcagaccag cagggcgtct
     3001 ttcgtttcga ccaccaggat atcctttatt ccgatgagcg ccaccagctt gttcgggctg
     3061 tatacgagcg tgttttcggc gttctcgtac atagcctgcg aaccgcccgc gagcgcgttg
     3121 cctttgaagt ccttctggga gatttcccac agtgtatccc aacttcccac gtcgctccag
     3181 ccgaagccgg ccggaatcat gaagacgtct ttggcttttt ccatgacgcc gtagtcgatg
     3241 gagacggacg cgaggcttct gtagattttt gacaccgttc gggcttcgga agatgttccc
     3301 aaagaagatt taatcgtcat cagccccgaa tgcagatccg gcaggaattt ttcaatttct
     3361 ttcaggatgg ttgaggcatt ccagataaac atgccgctgt tccagaaaaa attgccgttt
     3421 tgcagaaaga tccgggcctg ctccaggttc ggcttttcgc gaaacgcttt gacccgcagc
     3481 agcttttcgc ctgcaacatt tcctgccgaa gcgctgcctt ccatgtagcc gaacccggtg
     3541 tgcgggctgg cgggagtgag gccgatggtg accagggcgc ttttcttttc ggcggcccgg
     3601 gccgctgcgg cgatgacgct gcggtatcgt gcaggatcgg cgatgccgtg gtcggacggc
     3661 agaacgacca tgacatcgtc tttgactttt ttctgaatgt ggagagcggc gagcccgatg
     3721 caggcggccg tgtttcgccc gaccggttcg ataatgatgt ttttcgcggg gacttccggc
     3781 aattgtctca tcagctcccg cgcatgtttt ttccccgtta cgatcagaat gtttttgggt
     3841 ttgatgagcg gcttgatccg gtcgacggtt tcctgaatga tcgtccggtc gctgacgata
     3901 tccagcagat gctttggctt cttttcccgg ctgcgcggcc agaaccttgt t
//
LOCUS       DAOSIM010000190         3905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_20463_length_3905_cov_6.134026, whole genome shotgun sequence.
ACCESSION   DAOSIM010000190 DAOSIM010000000
VERSION     DAOSIM010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20463_length_3905_cov_6.134026"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLR47_08215"
     CDS             complement(<1..219)
                     /locus_tag="PLR47_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOG12969.1"
                     /translation="MTYRETYLRWKECVAEPDLLAELEAIQNDDAQIQDRFLRDLPFG
                     TAGLRGVIGAGIARMNRYVVGRATQGLAD"
     gene            complement(269..2269)
                     /locus_tag="PLR47_08220"
     CDS             complement(269..2269)
                     /locus_tag="PLR47_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOG12970.1"
                     /translation="MLINYKKEMMHDSTMPEYRDPAGAVVTGTPVVLRFRTKLENVES
                     VYLCLIGDGFHEDHQMRLAGDAWQTEITAPSIPDVYWYYFMVRVGGRLSYYGAASGRT
                     CGIGAVYSAPPPAYQLTVYNAAFEVPGWFQKSVMYQIFPDRFHFGGDSTVKKGIAHHR
                     SKGRSVYLHDTWDEPPLYAPLPGETAYNPCDYFGGTLSGIEASLDYLQSLGVTVLYLN
                     PIFEAASNHRYNTADYFRIDPVLGTEEDFRSLCRAAEERGMRILLDGVFSHTGSDSIY
                     FNREKAYDSPGAANSKDSPYYTWYTFEHYPDQYRCWWGFTSLPEINKQDSGWRRYVIT
                     GSDSVVKHWLQAGAAGYRLDVADELPDDVLAKIYAAAKQTRPEAVMIGEVWEDATTKH
                     SYGVKRRYALGGLLDSVTNYPLRSALIAFLKGQSDAFDLKHFLVDQAANYPAPLYYAL
                     TNLLSSHDVERVRTALSARIDPHQLTREQQASFVVSDSQNKKGARRQRLAAVLQFCLP
                     GVPCVYYGDEKGMTGFLDPFNRAPFTRKAPYDLTEEYRRLAGLRASADALRTGRVVYF
                     APDADCLGVLRYITGGKDALGRPAEDGVYFMAVNRSDARRLVVFDFSGKHSLLCEADQ
                     RSLRPLFNGTFTCRLTQKSFTFIDALLEISLDPLGALWLKIA"
     gene            complement(2306..>3905)
                     /gene="glgP"
                     /locus_tag="PLR47_08225"
     CDS             complement(2306..>3905)
                     /gene="glgP"
                     /locus_tag="PLR47_08225"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005977 - glycogen metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HOG12971.1"
                     /translation="QYYFLASATMQCIVREHKKRFGDVHTLPDKIVIQINDLHPALAI
                     PELIRILIDEEGLGWDDANVIAGRVFNYTNHTVMQEALAAWPEHVFQSMLPRVYAIVA
                     EISERFSRQLWKVFSQDRDKISRLAIIAYDEVRMANLCLAVCRKVNGVSQLHGRILKT
                     RTFRDFYVMFPGKFTAVTNGITQRRWLAAANPALCALIADHIGDKFLRDYNELERLRP
                     LADKPRFLADFAEVKRGNKRRLAEQVAKQQGIVLDPDSVFDVQAKRLHEYKRQLLKVL
                     HILHLYNCFTSDAGYALPRPVTFLFAAKASPAYWRAKSIIRLINAAAALVESHPRTRD
                     NIRVAFLENYNVSLAERLIPAADISEQISTAGYEASGTGNMKFMLNGAVTLGTMDGAN
                     VEIFERVGRDNIFIFGASAGEIDRMEIDNSYRPKDLFDHNEDIRAAVSRLVDGTLPGV
                     APDQFQDIYQSLLTGDYDRADRYFLLYDFDPYRAAFENVLAAYTDEPAWTKRAVLNTA
                     GAGFFSADRTIDEYNRQIWGLRPI"
BASE COUNT          815 a         1157 c         1134 g          799 t
ORIGIN      
        1 atccgccagt ccctgcgtcg cccggccgac gacatagcgg ttcattcggg caatgcccgc
       61 gccgataacg ccgcgcagtc ccgccgttcc aaacggcagg tccctcagga aacggtcctg
      121 aatctgtgcg tcatcgttct gaatggcctc cagttcggcc agcaggtccg gttccgcaac
      181 gcattctttc cagcgcaaat aggtttcccg gtacgtcata atacctctcc ctttttgatt
      241 ggttccatct ccaggctctg tgcacccttc aggcgatctt cagccacaag gccccgaggg
      301 gatccagcga aatttccaaa agcgcgtcga tgaaagtgaa ggacttttgc gtcaaccggc
      361 aggtgaacgt gccgttgaac agagggcgaa ggcttcgctg atccgcttcg caaagaagcg
      421 aatgctttcc cgaaaaatca aagaccacca gccgcctcgc gtccgaacgg ttgaccgcca
      481 tgaaatagac gccatcctcg gccggccggc ccagagcgtc ttttccgccg gtaatgtagc
      541 gcaaaacgcc gaggcagtcg gcgtcggggg cgaagtacac cacgcggccc gttcgcagcg
      601 cgtctgccga agcgcggagc ccggccagcc gccggtattc ttccgtcagg tcgtacggcg
      661 ctttccgggt aaaaggcgcg cggttgaaag gatcgagaaa tcctgtcatt cctttttcgt
      721 ctccgtaata gacgcaggga acgccgggga ggcagaactg cagcaccgcg gccagccgct
      781 ggcgcctcgc gccttttttg ttctggctgt cgctgacgac aaaggaagcc tgctgctccc
      841 gggtcagctg atgaggatcg atgcgcgcgc tgagcgctgt gcgcacacgc tccacgtcgt
      901 gggaggaaag cagattggtc agggcgtagt aaaggggagc gggataattg gccgcctgat
      961 ccaccaggaa atgcttcagg tcaaaggcgt cggactggcc cttcagaaaa gcgatcaggg
     1021 cgctgcgcag cgggtaattc gtgacggagt ccagaaggcc tcccagcgca tagcgccttt
     1081 ttacgccata gctgtgcttc gtggtcgcgt cttcccaaac ctcgccgatc atcaccgctt
     1141 ccggccgggt ctgttttgcg gcggcgtaaa tcttcgccag gacgtcgtcg ggcagctcat
     1201 cggccacatc gagacggtag cctgccgcgc ccgcctgaag ccagtgcttc acgacgctgt
     1261 cggagccggt gatgacgtat cgccgccagc ctgaatcctg cttgttgatt tcgggcaatg
     1321 acgtaaaccc ccaccagcag cggtattgat cgggataatg ctcaaacgtg taccacgtgt
     1381 aatagggaga atccttgctg ttggcggcgc ccggactgtc atacgctttt tcccggttaa
     1441 aatagatgct gtcggagccg gtatgggaaa acacgccgtc cagaagaatg cgcattccgc
     1501 gttcttcggc cgcacggcac agggagcgga aatcctcctc ggtgcccagc accggatcga
     1561 tgcggaaata gtcggcggta ttgtaccggt ggttggaggc cgcctcaaag atcggattca
     1621 aatacaacac cgtcacgccc aggctttgca gatagtccaa cgacgcctcg atgccgctca
     1681 gcgtaccgcc aaagtaatca caggggttat aggccgtctc ccccggcagc ggagcataga
     1741 gcggcggttc gtcccacgtg tcgtgtaaat agacgctgcg gcccttggaa cgatgatgcg
     1801 caatgccctt ttttaccgtc gagtctcctc cgaagtgaaa acgatctgga aaaatctgat
     1861 acattacgct tttctgaaac caaccgggaa cctcaaaagc ggcattgtaa acggtcagct
     1921 ggtatgcggg cggcggcgcg ctgtaaacgg cgccgatgcc gcaggttcgt cccgatgcag
     1981 cgccgtaata gctgagtctt ccccccacgc gcaccatgaa ataataccag tacacatcgg
     2041 gaatcgaagg cgctgtgatt tccgtctgcc aggcatcgcc cgcaagccgc atctgatgat
     2101 cttcgtgaaa gccgtctccg atcaggcaca gatagacgct ttccacattt tccagctttg
     2161 tccgaaagcg cagaaccacc ggcgtgcccg tgacgacggc ccctgcagga tcgcggtatt
     2221 ccggcatcgt cgaatcatgc atcatttctt ttttgtagtt gatcagcatc gctgccgtcc
     2281 ttttgcgcgc ttcagattgc gcacatcaga tgggccgaag cccccagatc tgacggttgt
     2341 attcgtcgat ggtccggtcc gcgctgaaaa atcccgcgcc ggctgtgttg agcacggccc
     2401 ttttcgtcca ggccggctca tccgtgtaag cggcaagcac attttcaaac gcggcgcggt
     2461 aagggtcaaa atcgtagagc agaaagtaac ggtcggcgcg gtcgtaatcg ccggtgagca
     2521 gggactggta aatatcctga aactggtcgg gcgcgacgcc cggcagcgtt ccgtccacca
     2581 aacgggaaac ggccgcccga atgtcctcgt tgtggtcaaa gagatccttc gggcgatagc
     2641 tgttgtcgat ttccattcgg tcaatctcgc ctgcgcttgc gccaaaaatg aagatgttgt
     2701 cccgccccac cctttcaaag atttccacat tcgcgccgtc catggttccg agcgtcaccg
     2761 cgccgttgag catgaatttc atattgcccg tgccggaggc ttcatacccc gccgtggata
     2821 tctgttcact gatgtccgcc gcgggaatca ggcgttcggc cagcgagacg ttgtaatttt
     2881 ccaggaaagc gacacgaatg ttgtcccgcg tgcgcggatg cgactccacc agagcagccg
     2941 cggcattgat gagccggatg atgctcttgg cccgccagta ggccggcgaa gccttggcgg
     3001 caaacagaaa cgtcaccggc cggggcagcg cataccccgc gtcggaggta aagcaattgt
     3061 acagatgaag aatgtgcagc actttcaaca gctggcgctt gtattcatgc agccttttgg
     3121 cctgcacgtc aaagaccgag tcgggatcaa ggacaatgcc ctgctgtttg gccacctgtt
     3181 cggccagacg ccgcttgttg ccgcgcttga cttccgcgaa atcggccaga aaccgcggct
     3241 tgtcggccag cggcctgagc cgctccagct cgttgtagtc ccgtagaaat ttatcgccga
     3301 tatggtcggc aatcaacgcg caaagggcgg ggttcgccgc ggccagccag cgccgctggg
     3361 tgatgccgtt ggtgacggcg gtgaatttcc ccggaaacat cacatagaaa tcccgaaagg
     3421 tccgcgtctt cagaatccgg ccgtgaagct gcgagacgcc gttaaccttg cggcagaccg
     3481 ccaagcagag attggccatg cggacttcgt catacgcgat aatggccaga cggctgattt
     3541 tatcgcggtc ctgtgaaaag actttccaga gctgccggct gaagcgttcg gatatttcgg
     3601 cgacaatcgc gtaaacgcgc ggcagcatcg actgaaaaac atgctcgggc caggcggcca
     3661 gagcctcctg catgacggtg tgattggtgt agttgaacac gcggccggca atgacgttgg
     3721 cgtcgtccca tcccaacccc tcttcgtcga tcagaatgcg aatcagctcg ggaatggcca
     3781 gcgccggatg caaatcattg atctgaatga cgattttgtc gggcagcgtg tgcacgtcgc
     3841 caaagcgctt tttgtgttcg cggacgatgc actgcatcgt ggcggaagcc agaaaataat
     3901 actgc
//
LOCUS       DAOSIM010000191         3897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_20527_length_3897_cov_6.996877, whole genome shotgun sequence.
ACCESSION   DAOSIM010000191 DAOSIM010000000
VERSION     DAOSIM010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20527_length_3897_cov_6.996877"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..1001)
                     /locus_tag="PLR47_08230"
     CDS             complement(279..1001)
                     /locus_tag="PLR47_08230"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOG12972.1"
                     /translation="MKRIILFILVMAALLCLPYLVTGSRTAEAAKPAKASCQSCHADF
                     AKVVPKEHPPVKGNDLSACTSCHAPDLAGTAQKNTFSARMHRAHVPPKGSLDCTACHT
                     WTPGKNFGLIGHKGSRGAPSKDDMAMMKEIFSSWAGSNYTDNLHARAGITCTQCHGKD
                     IPKADDTVENGRCLACHGPLDKLAQKTEPKDFKDRNPHKSHLGDIACTVCHKGHAESK
                     VYCLSCHPNFKMKIMGASQAKQ"
     gene            complement(1065..2627)
                     /locus_tag="PLR47_08235"
     CDS             complement(1065..2627)
                     /locus_tag="PLR47_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HOG12973.1"
                     /translation="MKEEIGEKVENKGMSRRSILKGAAALGVIAAGGGLALNMAAPGT
                     AEAMVRKMPKKWDETWDVVVIGSGFAGLAAAAEAAGAGAKVVILEKMPIYGGNSIING
                     GGYGAYDDKLKLRKKLNLGDDTAKQHFDDTLKGGDYYNIPELVQVLVNESPDALNWMI
                     DEGGLKLRDVLTRGGGHTAYRCHFSETMWGKDYTEALKKIAEKRGAKMILGSEVTWIW
                     RKDADPKSPVLGLEVKRGRRTLNVKAQKAVVLASGGFSQDVKMRMEHYPKLTKDFNCT
                     NQKGATGEMIRFAKAVGADTLQMNFIQLYPFADPNTGTLDTPAVFPFSGPGRGAIYVN
                     KNGKRFVSELERRDVCAFAQTNLGPGMKPTYTIVSAAMIPLMGYKQEEVDAGLRKGRL
                     VMADSIGELAKKIGLPSDVFEKTVSDHNRYLQEGKDPDFNKPITKAMLPLDKGPFYAV
                     AQWPAVHHTMGGLRINKNAQVIDIFGAVIPRLYAAGEVAGGVHGSNRLGTNATADCVV
                     FGRVAGKNAAKA"
     gene            complement(2864..3100)
                     /locus_tag="PLR47_08240"
     CDS             complement(2864..3100)
                     /locus_tag="PLR47_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOG12974.1"
                     /translation="MHDLVKLSRKENDIASDTLLNWFSKEQVEEEATAEKILRQMEMI
                     GDSKQGIYLLDRDLATSNFTAGSPIDPAAYNLFN"
     gene            complement(3617..>3897)
                     /locus_tag="PLR47_08245"
     CDS             complement(3617..>3897)
                     /locus_tag="PLR47_08245"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG12975.1"
                     /translation="NDFNRVIELTPRNAKAYLGRGVCQAFQGQFSKAIEDLNKSIELD
                     PKLPDAYNIRGMVYAQIGNPRAISDFQKVCDMGHAQGCSNWQRILKSR"
BASE COUNT          824 a         1074 c         1010 g          989 t
ORIGIN      
        1 ggaaaccctt gatatcagtc cactggcata gttacaactt taacgggaat ttaggtttat
       61 atacatgaat tcataaaatg acaaaccatt tgtcatgaaa ggatggacaa tcccgcatcg
      121 ttttcggctt tgtggcttcc gtgctgcccc ctttccggcc ggcgaaaaac tctttacaca
      181 accgctgatc aatgtggcag agcggattca caaagaaaaa cagggctgaa gagacaatgt
      241 ttcctcagcc ctgctcaagt ggttttaccc tgagtatgtt attgttttgc ttgagatgcg
      301 cccatgatct tcatcttgaa gttcggatgg caactcaggc agtaaacctt ggattccgcg
      361 tgccccttgt ggcagaccgt gcaggcaatg tcgcccagat gcgacttgtg gggattgcgg
      421 tccttgaaat ccttcggttc cgttttttgt gccagtttgt ccaggggacc gtgacaggcc
      481 aggcaacggc cgttctccac cgtatcatcg gcctttggaa tatccttgcc gtggcactgg
      541 gtgcaggtga tccccgcccg ggcatgaaga ttgtccgtgt aattggagcc ggcccaggag
      601 ctgaaaatct ccttcatcat ggccatatcg tctttgctcg gcgcccccct ggagcctttg
      661 tgcccgatca acccgaaatt cttccccggc gtccaggtat gacaggccgt gcaatccagt
      721 gagcctttcg gcggcacatg cgcccggtgc atccgagccg aaaaggtgtt tttctgtgcg
      781 gtacccgcca gatccggtgc atggcacgat gtacaggccg aaagatcatt cccctttacg
      841 ggagggtgct ccttgggaac gaccttggca aaatcggcat ggcagctctg gcaagatgcc
      901 ttggccggtt tggccgcctc ggccgtgcgg ctgccggtca ctaaataggg caggcacaac
      961 agggcggcca taacgagaat gaataagatg attcttttca tggtcaacct tccttgatga
     1021 tgaaaagaaa ggggaccatg gtttccatgg ccccccgtaa cgcatcaagc ttttgctgca
     1081 ttctttccgg caacgcgacc gaagacgaca caatctgccg tcgcgtttgt tccgagcctg
     1141 tttgacccat gaacgcctcc ggcaacttct ccggctgcat agagtctcgg gatgacagcg
     1201 ccaaaaatgt caatgacctg ggcgttcttg ttgatcctca ggccgcccat ggtgtgatga
     1261 acggccggcc actgggcaac ggcgtaaaag ggtcccttgt cgagaggaag catcgctttg
     1321 gtaatcggct tgttgaaatc gggatctttt ccctcctgca gatagcgatt gtggtcgctg
     1381 accgtcttct caaagacatc ggaggggagg ccgatttttt tcgcaagctc tccgatcgaa
     1441 tccgccatga caaggcggcc tttcctcaag ccggcatcca cttcctcctg cttgtaaccc
     1501 atcaggggaa tcatggcggc gctgacgatg gtataggtgg gcttcatgcc gggtcccagg
     1561 ttggtctgtg caaaggcgca gacgtcccgg cgctccagtt cactgacaaa acgtttcccg
     1621 ttcttgttga cataaatagc accgcggccc ggaccgctga aaggaaacac ggcaggagta
     1681 tccagggtgc cagtattcgg atcggcaaag ggatagagct ggatgaaatt catctgcagg
     1741 gtatccgcgc cgacagcctt ggcaaaccgg atcatttcgc cggttgcacc cttctggttc
     1801 gtgcagttga agtccttcgt gagcttggga tagtgttcca tccgcatctt gacatcctgg
     1861 ctgaacccgc cggaagccag aacaaccgcc ttttgggcct tgacattcaa cgttctcctg
     1921 ccccttttga cttccaaacc gaggacgggg ctcttcggat cggcgtcttt gcgccatatc
     1981 caggtaacct ccgaaccgag gatcattttt gccccacgct tctccgcgat ctttttcagc
     2041 gcttcggtat agtcctttcc ccacattgtt tcagaaaaat ggcaacggta agccgtgtgc
     2101 ccgccgcctc tggtcaagac gtcacgaagc ttgagaccgc cttcatcgat catccagttc
     2161 agggcgtccg gcgattcgtt gaccagaacc tgcacaagct ccgggatgtt gtaataatcg
     2221 ccccctttga gggtgtcgtc gaaatgctgt tttgcggtat cgtcgcccag gttcagtttc
     2281 ttacgaagtt tcaatttgtc atcataggcg ccgtagccgc cgccgttgat gatggagttt
     2341 cctccataga tgggcatttt ttccagaatg accactttgg ctccagcccc cgccgcttcg
     2401 gctgccgctg ccagtcctgc aaacccggag ccgatcacca cgacatccca ggtctcgtcc
     2461 catttcttgg gcattttcct gaccattgcc tccgccgtcc ccggtgcagc catgttcagg
     2521 gccagacctc cgcccgccgc gatcacaccc agggcagcag cccctttcag gatggaacga
     2581 cggctcatgc ctttgttttc gactttttct ccgatttctt ctttcattat ggtacctcct
     2641 catatcgaat gaaaattgtt tgattgaaaa ttaaatcatc tccgtgacat cgacatcccg
     2701 gaaccctatc caaacagatt gaatcccttt ttgtagaaaa acgcgcctca tgccgtttca
     2761 tggataaaat attaagaaca cggacatgct ctgtccatca gacaaaccat aaaaaaagca
     2821 taggatttca taacccacct gaagcagcag ctgatcgatt caatcagttg aacaagttgt
     2881 aggcggccgg atcgatcggc gatcctgccg taaagttgct ggttgccaga tcccggtcca
     2941 gcagatagat tccctgcttg ctgtccccga tcatctccat ctgcctgaga atcttttccg
     3001 ccgtggcttc ctcttccacc tgttctttgg aaaaccagtt caggagcgtg tccgaggcga
     3061 tgtcgttctc cttccgggaa agcttgacca gatcatgaat ctttgccgta tatcatatat
     3121 atattgataa gaacttgcct ttgacggttc gtagtatagg aaagccattt tctcgtgtca
     3181 aggggtaatt ttcaccggaa atcaggggtt ggttatgtgg tcgggcagtg tatgttgatt
     3241 ggcggaggtc cttctggcgc catcggctct gtttccggac gaattgcccg tcaggtaggc
     3301 attttgaggc gcttttcgga cagacctctg ctgcacgcct caatttttca aagcgtcatg
     3361 cctggatgta atcgtcccag gagtattggg ggtctccata gaaatgatcg tcatgggcgg
     3421 ggatctgtga gacgtcatcc ggaatggata gggcagccgg cctgcctgcg ttatgtatgc
     3481 agaccggcag gcaatggatt ttcggcggcg gccttgctct gacaagccag atgcccagat
     3541 gttcgaggat ggcccggatc acctgtctgt cttcgatgaa aaagaacggc gcctatcaca
     3601 tcgccgtcaa agtagatcac cggctcttca atatccgttg ccagttgctg cacccctggg
     3661 cgtgtcccat gtcacagacc ttctgaaaat ccgatattgc ccggggattg ccgatctgag
     3721 catagaccat ccccctgatg ttataggcat caggtaattt tggatcgagt tcgatggact
     3781 tgttgagatc ctcgatcgct ttactgaact ggccctggaa ggcctggcag acaccacggc
     3841 caagataggc cttggcattg cgcggcgtca gttcgatgac ccggttgaaa tcgttgg
//
LOCUS       DAOSIM010000192         3892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_20562_length_3892_cov_7.444618, whole genome shotgun sequence.
ACCESSION   DAOSIM010000192 DAOSIM010000000
VERSION     DAOSIM010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20562_length_3892_cov_7.444618"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLR47_08250"
     CDS             <1..254
                     /locus_tag="PLR47_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12976.1"
                     /translation="IPGFPKDYLCYIMGVSRIPALTFIIISTIGRFFGTMMLSVSGNM
                     AREEHYAMLAVIGVASAVVAFLAWRYHEKILELLKNHKG"
     gene            299..1282
                     /locus_tag="PLR47_08255"
     CDS             299..1282
                     /locus_tag="PLR47_08255"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOG12977.1"
                     /translation="MRIQKPRSLNEKTGRSAAAPAVWLRALRLHFVLPSILPALLGGI
                     MAWAAGHPLNGLHFALVVIGVTFNHFGLNLVDDVLDYRHTVDLQAGGEKNFFTGGSGV
                     LPEGLLKDYQMLTAASLFFAATVVIGIYLAWACGWPVLVFGVFGMACSIFYTAPPIRF
                     GYRGFGELGLLVNFGPVIVLGAYFVQARTLAIEPFIASLVPGLLMASMILINEIPDYE
                     ADRRGGKWNLVARFGRKTGALLYGACLACACGILVLSSVAGVLSPFALLGLVSLPFAA
                     KSLAVLRRHLNDPLAMAPANLAMIKVHALTVAAMVAAYLLEVLIAARKFIC"
     gene            1311..2279
                     /locus_tag="PLR47_08260"
     CDS             1311..2279
                     /locus_tag="PLR47_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG12978.1"
                     /translation="MISEISENFYRITLPMPFRLRHVHAYMLVESGRAVLFDAGMLMP
                     GAFEKLEADLTGIGIAAADICDIYLTHTHTDHCGLAGRIQEKSKARLHLSNEARQVYD
                     RFQQGEDLIARARLFYARHGLPAEDVEAIVEEIEDLRGRIPLLEVATLLRDREIRRFG
                     SRAFEVIFTPGHAAGHVCFFFRDEGLLLAGDHILPYIAPSLSPNIDDEVFQPLASYLE
                     SLAVIEKLPISIIHPGHGNSFNGVHERIREIREHHALKKDMLQRRIGALPQTTFGICK
                     ETMRTGADGWEQFMALNETYVYLQHLKREGAIREGMVNNTLVYSAV"
     gene            complement(2290..2766)
                     /locus_tag="PLR47_08265"
     CDS             complement(2290..2766)
                     /locus_tag="PLR47_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12979.1"
                     /translation="MLKDYQEKILELLEELELNMCNLYLLFADRFPKYRDLWADISRQ
                     ETSHADGIKKLHLLAGEDRVLFDEKLTKTYTVKMFITSIQEAQTRTKSGKMSLINALS
                     VSHDFEQAVLEKGFYNFFSGQDDETRTVIRQIKEETLDHQMKMKNAWEQERKSAGP"
     gene            complement(2901..>3892)
                     /locus_tag="PLR47_08270"
     CDS             complement(2901..>3892)
                     /locus_tag="PLR47_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG12980.1"
                     /translation="MDACFKNEDLKAVVSQLWVFYGAPVDKESALIFMAATESYLSDG
                     AWHIKGTSQALADGYAARIRELDGTVQTGTLVTKILLENGRATGVQTANGKTYTARYI
                     VANTDPYQLADKLVGRENLPAKYLEKIDAMKPANSLFGVYIGLNVDLKALGYNDTEIF
                     YNTYRDSMKNYEAMMKGDFEKGAVAITIYTNLGDPVYAPKGKSVVKLDAYSDISVWPK
                     DRAEYAKLKEQKVDELIALAARVIPELKDPKNITVKEGYTPKTIERFTMNKGGVVYGF
                     YLSPEQWQKIPNDTPIKNVFITSNWTQAWHGVCAGQVNGWRAARLILDREGIE"
BASE COUNT          740 a         1078 c         1100 g          974 t
ORIGIN      
        1 tgattcccgg ctttcccaag gattatctgt gctacatcat gggcgtcagc cgcattccgg
       61 cgctgacctt tatcattatt tccacgatcg gccggttttt cggcacgatg atgctgtcgg
      121 tcagcggcaa tatggcgcgc gaggagcatt acgcgatgct ggccgtcatc ggcgtcgcgt
      181 ccgccgtggt tgcgtttctg gcatggcggt atcacgagaa aattctggag ctgctcaaaa
      241 accataaggg gtaaaagccg ccgacaagcg gtccgtgcgg acgagacgaa ggcccgtcat
      301 gagaattcaa aaaccacgct ccctaaatga aaagacaggc cgaagcgcgg ccgcgccggc
      361 ggtctggctt cgggcgctgc gccttcattt tgttctgccc agtattctgc cggcgctctt
      421 gggagggatc atggcctggg ccgctgggca tcccctgaac ggtcttcatt tcgccctggt
      481 cgtgatcggc gtcaccttca atcatttcgg gctcaacctg gtggatgatg ttctggatta
      541 ccggcatacg gttgacctgc aggcaggcgg cgagaagaat ttttttaccg ggggcagcgg
      601 cgtcctgccg gagggactgc tgaaagacta tcagatgctg acggcggctt cccttttttt
      661 tgcagcaacg gtcgtcatcg gcatttatct ggcgtgggcc tgcggatggc cggtgctggt
      721 tttcggagtg tttggcatgg cctgctccat tttttacacc gcgccgccga tccgcttcgg
      781 ctatcgggga tttggagaac tgggcctgct cgtcaatttc ggccccgtga ttgttctggg
      841 ggcgtatttt gttcaagcgc ggacgctggc catcgaacct tttatcgctt cgctggtgcc
      901 ggggctgctg atggcgtcga tgatcctcat caacgaaatt ccggattacg aagcggaccg
      961 gcgcggcgga aaatggaacc tcgttgcccg cttcggaaga aaaacgggtg cgctccttta
     1021 tggggcctgc ctggcgtgcg cctgcggcat tctggtcctc tcgtccgtgg ccggggtttt
     1081 gtcccctttt gcgctcttgg gccttgtcag tcttcccttt gccgcaaagt cccttgccgt
     1141 cctgcgacgc cacctgaacg atccgctggc gatggcgccg gccaatctgg cgatgattaa
     1201 agtccacgcc ctcacggttg ccgccatggt tgccgcctat cttctggaag ttttgattgc
     1261 agcccggaaa tttatttgtt aaacattcac aaaccataag gctgcctttg atgatttccg
     1321 aaatttccga aaacttctat cgcatcaccc tgcccatgcc gtttcgcctg cggcacgtcc
     1381 acgcctacat gctggtggaa agcggccggg cggttctctt cgatgcgggt atgctcatgc
     1441 ccggcgcctt tgagaaattg gaagcggatc tgacgggcat cggcattgcc gcggccgata
     1501 tttgcgatat ttacctgacc cacacgcata ccgatcactg cggcctggcc ggccgaatcc
     1561 aggaaaaatc aaaagcccgc cttcatctgt cgaatgaagc ccggcaggtt tatgaccgtt
     1621 ttcagcaggg agaagacctg atcgcgcggg cccgcctctt ttacgcccgg cacggcttgc
     1681 ccgcggaaga tgtggaagcc atcgtggagg aaattgaaga cctgcgcgga cggattccgc
     1741 tcctcgaggt tgccacgctt ttgcgggacc gggaaatccg gcgctttggc agccgggcgt
     1801 ttgaggtgat cttcacgccg gggcacgcag cggggcatgt ctgcttcttt tttcgtgacg
     1861 aggggctttt gctggcgggg gatcatattc tgccctacat tgcgccgagc ttaagcccga
     1921 acatcgacga tgaggttttc cagccgctgg cgagctacct ggaatcgctg gcagtcattg
     1981 aaaaactgcc catcagcatc attcatccgg ggcatggaaa ttctttcaac ggcgttcacg
     2041 agcggattcg ggagattcgg gaacaccacg cgctgaaaaa agacatgctg caaagaagga
     2101 ttggcgccct cccccaaacc actttcggca tctgcaaaga aacgatgaga acgggcgcgg
     2161 acggctggga gcaattcatg gcgctcaacg aaacttacgt ttacctgcag cacctcaaac
     2221 gggaaggcgc aattcgtgaa ggtatggtga ataataccct tgtttattcg gcggtttgac
     2281 aattcggaat tatgggcccg ccgattttct ttcctgctcc caggcgtttt tcattttcat
     2341 ctggtgatcg agcgtttctt ctttgatctg ccggatcacc gttctggttt catcgtcctg
     2401 gccggaaaaa aaattataaa atcctttttc aagaacggcc tgttcgaaat catgactgac
     2461 cgacagagcg ttgatcagcg acattttgcc cgattttgtc cgggtctgcg cttcctgaat
     2521 gctggtgata aacatcttga ccgtgtaggt tttggtcagc ttttcatcaa acagaacgcg
     2581 atcttcaccg gccagaaggt gcaatttttt tattccatcc gcgtggctgg tttcctggcg
     2641 ggagatgtcg gcccataaat ctctgtattt ggggaagcgg tcggcaaaca gcagataaag
     2701 attgcacatg ttcaattcga gctcttccag aagctccaat attttttcct gataatcctt
     2761 cagcatcgcc gattacctcc gccgacttgc aatattcgcc cgcccccggg cgtcgaagca
     2821 taaaagctgc ctgaaaaaat catcagagcg gggccgcgga aatcttcacc tccgcggcgc
     2881 ccgcagagcc gtcgtccggt ttactcgatg ccttcccggt ccagaatcag acgcgccgcg
     2941 cgccagccgt tgacctggcc ggcgcatacg ccgtgccacg cctgcgtcca gttgctggtg
     3001 atgaatacat ttttaatcgg cgtgtcgttg ggaattttct gccactgttc cggcgacaga
     3061 taaaagccgt aaaccacgcc gcctttgttc attgtgaagc gctcgatggt tttcggcgtg
     3121 tagccttcct tgacggtgat gtttttgggg tctttgagtt cggggatcac ccgggcggcc
     3181 agagcgatga gctcgtcaac cttttgctcc ttcagtttgg catattccgc ccggtccttc
     3241 ggccagacgg agatgtcgga ataggcgtcc agcttgacca cggatttgcc tttcggcgca
     3301 tagacgggat cgcccaggtt tgtatagatc gtgatggcca ccgctccctt ttcgaagtcg
     3361 cccttcatca tggcttcata attcttcatg ctgtcccggt aggtgttgta gaagatttcc
     3421 gtatcgttgt agcccagggc tttcagatcg acattcaggc cgatataaac gccgaacagg
     3481 gaattggcgg gcttcatcgc gtcgattttt tccaggtatt tagccggaag attttcccgg
     3541 ccgacgagct tgtcggccag ttgataggga tccgtgttgg cgacaatata gcgggccgtg
     3601 taggttttgc cgttcgcggt ttggacgccg gttgcccggc cgttttccag aaggattttc
     3661 gtcaccagcg tgcccgtttg gacggttccg tccagctcgc gaatgcgggc cgcgtaaccg
     3721 tcggccagcg cctgggatgt gcctttgata tgccaggcgc cgtcgctcag gtagctttcg
     3781 gtggccgcca tgaaaatgag cgccgattcc ttgtccaccg gagcgccgta gaaaacccat
     3841 agctgggaaa ccaccgcttt aagatcctcg tttttaaagc aggcatccat ca
//
LOCUS       DAOSIM010000193         3863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_20753_length_3863_cov_8.682248, whole genome shotgun sequence.
ACCESSION   DAOSIM010000193 DAOSIM010000000
VERSION     DAOSIM010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20753_length_3863_cov_8.682248"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2251
                     /gene="cysS"
                     /locus_tag="PLR47_08275"
     CDS             <1..2251
                     /gene="cysS"
                     /locus_tag="PLR47_08275"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOG12981.1"
                     /translation="LNPNKKVTILAKLESANPGGSIKDRTALSMIENAEKRGDLRPGK
                     VILEATSGNTGIGLAMVAAAKGYKICLAMSEAASEERKKILRAMGAELIFTPAPLGTD
                     GAIEVAYRMLRENPEKYFCTDQFNNPDNVEAHYCGTAQEIWDQTEGRVTAVVVTLGTT
                     GTAMGLSKRLKELNPNIRIIGVEPYLKHKIQGLKNMRESYRPGIFDKKRLDEKVNILD
                     EDAFETARRLAREEGILAGMSSGAALFVAARKAREMEEGLIVVIFPDSGERYLSTELF
                     TVKEELATMSFYNALKRRKTLFRPLKPEEVRLRTCGPTVHDAPHLGNYRRLVVSDLLC
                     RYLAFKGYAVKHVVDIVDCGDKSIRGSEKAGLNLADYSGRYLRVFLEDVRFLRIRPEN
                     IYVKASEDADAALAIVGKLVDRGYAYEKLHSVYFDISKLGDYGSLSNVDLAKTRTGRS
                     VDLDDYEKDSPADFALLKRAGLSELKRGIYTKTKWGNVRPGWHLECAAISQKYLGAAY
                     DIHVSGADETFPHCENLVAISKACFGNGGAQYWIGAELVLVDGRKMSRSLNNIATMAD
                     LRQSGYGGRDVRFFLLGMNYRKPISYSENALRMAGNTVKKMDTLISRLRAVSHSANDF
                     PEVDQLIYDLNHGFEHALDDDLNISGALAALFDFIGAVNTPLAGGKISRQDASKILEA
                     LQKINDVLQIFEFKEQIDDQGIRELIQKREAARQAGLWEEADSLRNRLAGLGVEVLDS
                     PQGTIWHFR"
     gene            2263..3084
                     /gene="amrB"
                     /locus_tag="PLR47_08280"
     CDS             2263..3084
                     /gene="amrB"
                     /locus_tag="PLR47_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOG12982.1"
                     /translation="MENIRKSVIAGSWYPGDPAVLRREIEEYFRKVPVREISGEVAGL
                     IAPHAGYRYSGQVAAYAYNLVRGKKYDGVVLIGPSHRVAFPGVSVWGSGAFETPLGLV
                     PVHEALVREISRFDKNIIDFPAAHAQEHSLEIQLPFLQAALGDFSFVPLVMGDQSAAV
                     CRRLAEAVVRAGENRRLLLVASSDLSHFHEDEQARALDGVATRRLRDNDAEGLLEKLA
                     HDQTEACGGGPMAAVMMAAKRLGASHSLLLKYAHSGNVTGDNTSVVGYASAVFYQ"
     gene            3100..3648
                     /gene="amrA"
                     /locus_tag="PLR47_08285"
     CDS             3100..3648
                     /gene="amrA"
                     /locus_tag="PLR47_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOG12983.1"
                     /translation="MELTDQEKTALLEIARRAVIAKVANRELPRLSGDLPILKEKRGA
                     FVTLKKRGHLRGCIGYIKAVKSLGETVQEMAVAAAFHDPRFPSVKSEEIRDLKFEISV
                     LSPLRKIQSIDEIEVGNHGLFVVRGYNSGLLLPQVASEYGWDRETFLRETCFKAGLPH
                     KAWKDKETDIYIFSANYFSDRD"
BASE COUNT          883 a          998 c         1193 g          789 t
ORIGIN      
        1 gctgaatccg aataaaaagg tcacgattct ggccaagctg gagtccgcga atccgggcgg
       61 gtccatcaaa gaccgcacgg cgctcagcat gattgaaaac gccgaaaaaa gaggtgatct
      121 gaggcccggg aaggtgatcc tggaggccac cagcggcaat accggcatcg gactggccat
      181 ggtggctgcc gcaaagggct acaagatttg tctggccatg tccgaggcgg ccagcgaaga
      241 gcgcaaaaag attctgcggg cgatgggcgc cgagctgatc tttacccccg cgccgctggg
      301 gacggacggc gccatcgaag tcgcctaccg gatgcttcgg gaaaatccgg aaaagtattt
      361 ctgcaccgac cagttcaaca acccggacaa cgtcgaggct cattattgcg ggacggcgca
      421 ggaaatctgg gaccagacgg agggccgggt caccgccgtg gtggtcacgc tcgggacgac
      481 cggcaccgcc atgggccttt ccaaacggct caaggaactg aatccgaaca ttcgaatcat
      541 cggcgtcgaa ccgtacctga agcacaagat tcagggcctg aaaaacatgc gggagtccta
      601 ccgccccggc atcttcgaca aaaagcgcct ggacgaaaaa gtgaacatcc tggacgaaga
      661 cgcctttgag acggcccgcc ggctggcccg ggaagaaggc attctcgccg gcatgagttc
      721 cggagcggcc ctgtttgtcg cggcccggaa agcccgcgag atggaagaag ggctgatcgt
      781 ggtgattttc ccggacagcg gggagcgcta tctgagcacg gaattgttca cggtcaaaga
      841 agagctggcg acgatgagct tttacaatgc gctcaaacgc cggaaaacgc ttttccgtcc
      901 gctgaaaccg gaggaggtgc ggctgcgcac gtgcggcccg accgttcatg acgctcccca
      961 tctgggaaat taccgccgcc tggtggtatc cgacctgctg tgccgctacc tggcgttcaa
     1021 gggctacgcc gtcaagcatg tcgtcgatat cgtggactgc ggcgacaagt cgattcgggg
     1081 gtcggaaaag gcgggactga atctcgcgga ttattccggc cggtatttgc gggtgtttct
     1141 ggaggatgtc cggtttttgc ggatccgtcc ggaaaatatt tacgtcaaag cctccgaaga
     1201 cgccgacgcc gcgcttgcga ttgtcggcaa gctcgtcgat cggggctacg cctatgagaa
     1261 gctgcactcc gtttatttcg acatttccaa gctgggagac tacggcagtc tgtccaatgt
     1321 cgatctggcc aaaacccgca ccggccggtc ggtggatctc gatgattacg aaaaggacag
     1381 cccggcggac ttcgcccttt tgaaaagagc cggcctgtcc gagctcaaaa gggggattta
     1441 caccaagacg aaatggggca atgtgcggcc gggctggcat ctggaatgcg cggccatttc
     1501 gcagaaatac ctgggcgcgg cctatgacat ccacgtcagc ggggcggatg aaacgtttcc
     1561 gcactgcgag aatcttgtcg cgatcagcaa ggcatgtttc gggaacggcg gagcgcagta
     1621 ctggatcggg gccgaactgg ttctggtgga cggcaggaaa atgtcccgct ccctgaacaa
     1681 tattgcgacg atggccgacc ttcggcaaag cgggtacggc ggccgggatg tccgtttttt
     1741 cctgctgggc atgaattacc gcaaaccgat cagctattcg gaaaacgccc tgaggatggc
     1801 cggaaacact gtgaaaaaga tggacacgct catttcccgc ctcagggcag tgagccattc
     1861 cgccaatgat tttccggagg tggaccagct gatttatgat cttaaccacg gctttgaaca
     1921 tgcactggat gacgacctca acatttccgg cgctctggcg gcgcttttcg atttcatcgg
     1981 cgccgtcaac acgcccctgg ccggggggaa aatcagcagg caggatgcca gtaagatcct
     2041 ggaggcgctt caaaaaatca atgatgttct tcaaatattt gaattcaaag agcaaattga
     2101 cgatcagggg ataagggaac tgattcaaaa aagggaggcc gcaagacagg cgggcctctg
     2161 ggaggaggcg gattcgttga gaaaccggct ggccgggctc ggcgttgagg ttctcgacag
     2221 cccgcagggg accatctggc atttccggta ggggaaaggc aaatggagaa tattcgaaaa
     2281 tccgtcatcg cgggttcatg gtaccccggg gaccccgccg ttttgaggcg cgaaatcgaa
     2341 gaatatttcc gcaaggttcc cgtccgggag atttccgggg aagtggccgg tctgattgcg
     2401 cctcatgccg gctaccggta ttccggccag gtggcggcat acgcgtataa cctggtccgg
     2461 ggaaaaaaat acgacggggt cgttctcatc gggccctccc accgggttgc ttttccgggt
     2521 gtttccgtct ggggcagcgg agcttttgaa acccccctgg gcctggttcc ggtccatgag
     2581 gcgctggtgc gggaaatcag tcgtttcgac aagaatatca ttgattttcc ggcggcgcac
     2641 gctcaggagc attcgctgga aattcagctt cctttcctgc aggctgctct gggcgatttc
     2701 tcctttgttc cgctggtgat gggagaccag agtgccgcgg tctgccgaag gctggcggaa
     2761 gcggtcgtac gggctggcga aaaccggcgc ctgctgctgg tggccagcag cgatctgtcg
     2821 cattttcacg aggatgagca ggccagagcg ctggacggcg tcgcgacgag gcgcctgcgg
     2881 gacaacgacg cggaagggct tctggaaaaa ctggcgcacg accaaaccga agcctgcggc
     2941 ggcggtccga tggccgctgt catgatggcg gcaaagagac tgggcgcttc tcattcgctc
     3001 cttttgaagt acgcccactc agggaacgtg accggggata atacatccgt ggtcggctac
     3061 gcttctgcgg ttttttacca ataaaaaaaa gaggttgata tggagctgac agatcaggaa
     3121 aaaacggcgc ttttggaaat tgccagaagg gcggtcatcg ccaaggtcgc aaaccgcgaa
     3181 ttgccaaggc tctccgggga tttgccgatt ctgaaggaaa aaagaggcgc gttcgtcacc
     3241 ctgaagaaaa gggggcatct gcgcggctgc atcgggtaca tcaaggcggt caagtccctg
     3301 ggggaaacgg tgcaggaaat ggcggtcgcc gcggcttttc acgatcctcg ctttccgtcc
     3361 gtgaaaagcg aagaaatccg tgatttaaaa tttgaaatat cggtgctgag tcctctcaga
     3421 aagattcaga gcattgacga aatagaggtc ggaaatcacg gcctgttcgt cgtcaggggc
     3481 tataattccg gtttgctgct gccgcaggtg gcctccgagt acggctggga ccgggaaacg
     3541 tttctgcgcg agacgtgttt taaagcgggt ctgccccaca aggcatggaa agacaaggaa
     3601 acggatattt atattttctc cgcgaattat ttcagcgacc gggattgaat tttccccgtt
     3661 tcgtcaacgg aagaattttc caaataattt aaaaatttac ttgacaaacg cgaatataaa
     3721 aattagtgtc ctcaattcgc acgaagcggt gttgtcgctt ttttgcgttt aaagcggcgg
     3781 cggctttatg tgaaaacgta atgattcaga tggtttgctg gcgtagctca atcggtagag
     3841 cagctgattt gtaatcagca ggt
//
LOCUS       DAOSIM010000194         3843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_20878_length_3843_cov_9.016103, whole genome shotgun sequence.
ACCESSION   DAOSIM010000194 DAOSIM010000000
VERSION     DAOSIM010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20878_length_3843_cov_9.016103"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..743)
                     /locus_tag="PLR47_08290"
     CDS             complement(111..743)
                     /locus_tag="PLR47_08290"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOG12984.1"
                     /translation="MTTYAFAGFWRRFIAYMIDGFIVSVVIIFLGVVAGVAYIAGAMS
                     GDGSAIVAQMTDPDKLALLTAWIWLLTFAVNIAYFTYFHGSTGRTPGKRLLGLQVVSV
                     TGESISFGTAFLRSVGYLVSSLVFCLGYLWVAFDRRKQGWHDKIAGTVVIIREPRGNE
                     SGISIPDSSSGPQSPAGFGCVPVNVSQPEENKPDAPSVQGASGVDGQKMP"
     gene            complement(740..1513)
                     /locus_tag="PLR47_08295"
     CDS             complement(740..1513)
                     /locus_tag="PLR47_08295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOG12985.1"
                     /translation="MTLPRIYSPETLQNASQCVLGADQLRYLKTVLRLKPGDAVLVFD
                     GYGHEFEARIENFSPSGVAIRLGKLLPPVKRKIRLTLAQGIPKAGKMDGIVKTAAELG
                     TDVIIPVAAARSIRRIEGEKSSAKVARWQKIVQEAARCTRNSYVTVVEPVLSFADMLK
                     RADSGARKLIFWEEEDRQTIRNVLQDDRFSGAAHYFIIVGPEGGLAREEVDRARESGF
                     VSVSLGRQILKVETAAAAILSIIQYEKGIFSSVAEGGGK"
     gene            complement(1562..2506)
                     /gene="prmA"
                     /locus_tag="PLR47_08300"
     CDS             complement(1562..2506)
                     /gene="prmA"
                     /locus_tag="PLR47_08300"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOG12986.1"
                     /translation="MTQKEPEKWLKIQISASAEQIDALSNFLEETGAQGVFSESLMPP
                     GADDFPESSGLEVINAFFPADVRAEKRMKAVQKYLKSLEEIFPDAAVPVLSSETITDP
                     DWGEQWKKYFKPIRVCQNIVVKPTWERYAPDSRDIVVEIDPGMAFGTGQHASTRMCME
                     AIEDIILKDRSTGEWKVLDAGCGTGILGITAAKMGAQDVICVDNDPRATEIAAQNTVI
                     NAVEDRVQILNQDAARISRPRNLIIANLTAKLLLKLRPQLVRLLLPGGYLIISGIIEN
                     DVPAMEEHFIVAPLVQHRRLTEKEWVCCVLRKENGGPS"
     gene            2685..3425
                     /locus_tag="PLR47_08305"
     CDS             2685..3425
                     /locus_tag="PLR47_08305"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOG12987.1"
                     /translation="MQEPLVEPIRTDSLKEIFVLRFEELILSGKIKIGEKLPPERELA
                     LQLGVSRPVVHEGLVELASRGLVSIKPRFGATVNDYRKEGSIALMASLIQYQKGNLEP
                     ELLESLLQIRMMLEPPFARLAARNRTSEQILELHGILEKEAAANGRNPQALTDLDFEF
                     HLLIALASGNRVYPLLLNSFRSVYTNLSGQFFRNTKVTDAVHGYHEKMVNAVESGNET
                     RAASVMKAILNHGEKHLREMIGQESLSL"
     gene            3448..>3843
                     /locus_tag="PLR47_08310"
     CDS             3448..>3843
                     /locus_tag="PLR47_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12988.1"
                     /translation="MKTALANEVHLKEPKALSGRIQWLRDYYFRGTERPWNNEFTCWT
                     TGTPWDVIYNELTFYIVPETYTLLNTLGASYLQAARPVALHRDFWTWPLVERRAWFVK
                     EAIVNYVPQEILPGDLLAGARFNIQTSMCF"
BASE COUNT          843 a         1128 c         1042 g          830 t
ORIGIN      
        1 gccgacactc ggattttcag tccgatgctc taccgactga gctaccgacc cgaataacag
       61 gcaaaacctg tttaaatgcc gaaaacgtct ataaaacttg attccctttg tcaaggcatt
      121 ttttgtccgt ccactcccga cgcgccctgg acggaaggcg catcaggctt attttcttcc
      181 ggttgtgaaa cgttgacggg gacgcagccg aatccggcgg gactttgcgg tcctgatgaa
      241 gaatcaggga tcgaaattcc ggattcattt ccccgcggtt cgcggatgat cactaccgtg
      301 ccggcaatct tgtcatgcca gccctgcttt ctccggtcaa aggcaaccca gaggtatccc
      361 aggcaaaaaa ccaggctgga aaccaggtag ccgaccgatc tcaaaaaagc cgtgccgaaa
      421 gaaatcgatt cgccggtgac ggacaccacc tgcaggccca gcagcctttt tcctggtgtg
      481 cgtccggtgg agccgtgaaa ataggtaaag taggcaatat tgaccgcaaa cgtcagaagc
      541 cagatccagg cggtcagcaa agccagtttg tccgggtccg tcatctgggc gacgattgcg
      601 ctgccgtctc cggacattgc gccggcaatg taggccacgc ctgccacaac ccccagaaaa
      661 atgatcacga cgctgacgat gaatccgtcg atcatgtatg caatgaaacg ccgccagaag
      721 cccgcgaatg cgtatgtggt catttgccgc ctccttccgc gacggagctg aagatgcctt
      781 tctcgtactg gatgatcgac agaattgccg ccgccgctgt ttccactttt aaaatctgcc
      841 ggccaaggct cacggaaaca aagccgcttt ccctggcccg atccacttcc tcccgggcaa
      901 gcccgccttc cggtccgaca ataataaaat aatgcgccgc gccggaaaac cggtcatcct
      961 gcaaaacgtt ccgaatcgtt tgccggtcct cttcctccca gaaaatcagc ttccgcgcgc
     1021 cggaatccgc acgtttcagc atgtcggcaa aggacaggac cggctccacc acggtcacgt
     1081 aggaattccg cgtgcagcgc gccgcttcct gaacgatttt ttgccagcgg gcgactttcg
     1141 cggacgattt ttccccttcg attcttcgga tggaacgggc cgccgccacg gggatgatga
     1201 cgtccgttcc cagctccgcg gccgttttga cgatcccgtc catttttccc gctttgggaa
     1261 tcccctgcgc cagcgtcagg cggatttttc tcttcaccgg cggaaggagc ttgcccagac
     1321 gaattgcaac cccggacggg ctgaaatttt caatccgcgc ttcaaactcg tggccgtagc
     1381 cgtcaaaaac cagaacggcg tcgcccggct tcaggcgcag aaccgtcttc agataccgaa
     1441 gctgatccgc gccgagcacg cattgcgaag cgttttgcag cgtctcggga ctgtagattc
     1501 ggggcaacgt caacgtcaga cactttccag caaaaaagat tggcttgccg cctgaaagcg
     1561 atcaggacgg tccgccgttt tccttgcgca gcacgcagca gacccactct ttttctgtca
     1621 gacggcggtg ctgcaccagc ggcgcaacaa tgaaatgctc ctccattgcg ggcacgtcgt
     1681 tttcaatgat ccccgaaatg atcagataac cgccgggcag gagaagccgg acgagctgcg
     1741 ggcgcagttt gaggagcagt ttggccgtca ggttggcaat gatcagattg cgcggacggc
     1801 tgatgcgcgc tgcgtcctgg ttcaggattt gaacccggtc ttccacagcg ttgatgaccg
     1861 tattctgggc cgcaatctcc gtggccctgg gatcattgtc cacgcagatc acatcctgcg
     1921 cgcccatctt ggcggcggta atgcccagaa tgccggtgcc gcatccggca tcaagcactt
     1981 tccattcgcc agtcgagcgg tccttcagaa tgatatcctc tatcgcttcc atgcacatgc
     2041 gcgtggacgc gtgctgaccg gtcccaaacg ccatgcccgg atcgatctcc acgacaatgt
     2101 cgcggctgtc cggcgcgtat ctttcccagg tgggtttgac gacgatattc tggcaaactc
     2161 ggatcggctt gaaatatttt ttccactgct ctccccagtc gggatcggtg atggtttcgg
     2221 aagaaagtac gggcacggcg gcgtccggaa aaatttcttc cagacttttg agatattttt
     2281 gaaccgcctt catccgcttt tcggcgcgca cgtcggcggg aaagaaggcg ttgatgactt
     2341 ccagtcccga ggattcggga aaatcatcgg cgccgggcgg catcagcgat tctgaaaaaa
     2401 cgccctgcgc ccccgtttct tccagaaaat tgctcagggc gtcgatttgt tccgcggacg
     2461 ccgaaatctg aattttcaac catttttcgg gttctttttg cgtcatggcc cagccttcaa
     2521 tgaggtagtg tgaaaagcgg cttttatata gcctgttctt ccgaatatat caagcagccc
     2581 tgtcatggcc tgcggcctgc agaatttggc ttgacaaaaa cagtctcgga gcttattctg
     2641 acgctggtaa taccaccagc caggaaggcc gctgaggaga tatgatgcag gaaccccttg
     2701 ttgaacccat ccgcacagac agcttaaagg agatcttcgt gctccggttt gaagagctga
     2761 ttctttccgg aaaaatcaag atcggagaga agcttccgcc ggagcgggag cttgccctgc
     2821 agctgggcgt gagccgcccg gtggtgcacg aaggactggt cgaactggcg tcgcgcggac
     2881 tggtcagcat caaaccgcgc ttcggcgcca ccgtcaacga ttaccgcaag gaaggctcca
     2941 tcgccctgat ggcctcgctg attcagtacc agaaaggaaa tctggaaccg gagcttctgg
     3001 aaagcctgct gcagattcga atgatgctgg agccgccctt tgcgcggctg gcggcccgca
     3061 accggacttc ggagcaaatc ctggaactcc acggcatcct ggaaaaggaa gcggcagcca
     3121 acggccggaa tcctcaggcg ctcacggacc tcgattttga atttcacctg ctgattgcgc
     3181 tggcttccgg caaccgggtt tacccgcttc ttctgaattc gttccggtcg gtttatacga
     3241 acctttccgg ccagtttttc agaaacacga aagtaaccga cgccgtccac ggttatcacg
     3301 aaaagatggt caacgccgtg gaatccggca acgaaaccag ggcagcctcc gtgatgaagg
     3361 ccattctgaa ccacggggaa aagcatcttc gggaaatgat cgggcaggaa agcctgtccc
     3421 tgtaaccttt tttaccggga gggaaccatg aaaaccgcac tggcaaacga agttcatctg
     3481 aaagagccca aagcattatc cggccgcatt cagtggctgc gcgattatta tttccgcgga
     3541 accgagcggc cctggaacaa cgaatttacc tgctggacga ccggaactcc gtgggacgtg
     3601 atttacaacg agctgacgtt ttacatcgtg ccggaaacct acacgctgct caacacactg
     3661 ggcgcatcct atcttcaggc cgcgcggccc gtggcgctgc accgggattt ctggacatgg
     3721 ccgctcgtgg aacggcgggc ctggtttgtg aaggaagcca tcgtaaacta cgtgccgcag
     3781 gaaattcttc cgggggattt gctcgccggc gcgaggttca atattcaaac gtccatgtgc
     3841 ttc
//
LOCUS       DAOSIM010000195         3834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_20942_length_3834_cov_6.952104, whole genome shotgun sequence.
ACCESSION   DAOSIM010000195 DAOSIM010000000
VERSION     DAOSIM010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20942_length_3834_cov_6.952104"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLR47_08315"
     CDS             <1..188
                     /locus_tag="PLR47_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12989.1"
                     /translation="GGLLSGHPVIAAGLYSMAAVVRQIRGDAGGFQVQKAKTGLVQGV
                     NGLGGQSHCVWILDKDK"
     gene            200..1357
                     /locus_tag="PLR47_08320"
     CDS             200..1357
                     /locus_tag="PLR47_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12990.1"
                     /translation="MAKRVAIVGTGQTFHKSHRPDVNGQELIHEAVARALADADLKLK
                     DIDAVVIGNMDHFEGINYVDCWSVDGSGGTMKPIIKLTTGGTTGCTVAIGGYHLVGSG
                     MFDRVLIIGWEKNSESDTTGAITTAFDPIWDRLVFAGAISGLAAEAQAYMARYGATDR
                     DGARVSVRDRKHAMNNPHAQLRKEVSLEEVLASPMLADPIHLLDVCPRSDGACAVIMA
                     SEDIAEKICPKPDWIWGTAARHSYSYLGDADYGRLTSMREGSQELWKKAGIKEPRKEL
                     DVVEMYQPYSFGGLIWIEDMGLVGPGEAPRYIWDGNTDMGGELPINPSGGVISCNPIG
                     ATGLIRCAEAAMQVMGKAEGRQVPDVNFAFSSGFGGCWWTDMILHGKKKPA"
     gene            1378..1842
                     /locus_tag="PLR47_08325"
     CDS             1378..1842
                     /locus_tag="PLR47_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HOG12991.1"
                     /translation="MAEKENIIIESGDAVQPFAYSVGMHGSRFFAEIRDHKRFLGIKC
                     PKCGKVYIPPRGVCGDCFVEMKDWVEVGPKGVIGTFTILRYAFIDPETGAQKPVPYGY
                     GFIRFDGADTLFQHYINIEDEAKIRVGARVEPVFAGERKGSIRDIEYFRVID"
     gene            1842..2744
                     /locus_tag="PLR47_08330"
     CDS             1842..2744
                     /locus_tag="PLR47_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12992.1"
                     /translation="MAAKESPTVEINPFKRILKMPGALCGGISTGSDKIRSGYGNGDC
                     LYFDFEHLVFAVADGTERFPWASRDLLQRLSERLLRSGSPETARDWKDMMNTGVYDGQ
                     KYQHKTTFSAVALRREGEDVFLIIANGGDSVVTVMDGLTGKIRRQTGRNMDFAGRSRQ
                     IVDVTEHRMPDRDHRVLLSTDGFDDVWRFYLRRSPAGSARGVLERVGLDGIGEVISGL
                     LEEHRDRFEYDDVGFILLDPGAVKPVKGKALIMGGTRPFEENRYRLQYTPEAHDRWIP
                     DAEWAGQEEMLDGAGIRVLNTVRC"
     assembly_gap    2910..2919
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2920..3409
                     /locus_tag="PLR47_08335"
     CDS             <2920..3409
                     /locus_tag="PLR47_08335"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HOG12993.1"
                     /translation="EAGLSTGLVHYYFKNKEDLLFKLLKEMNANLRDNLKKAMGELAE
                     PRDKLMAFCDEAFALVDREKAYFYVLIDFWAQINHDARIRQANVKLFQSYRAEIAAII
                     QEGAAKGVFTVEDVNLTSVIIIALIQGTIIQYAIDREAFNYSEIKEKIKEQILSRIVN
                     GK"
     gene            3429..>3834
                     /locus_tag="PLR47_08340"
     CDS             3429..>3834
                     /locus_tag="PLR47_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG12994.1"
                     /translation="MEFGFTEEQLMFRDSVYKFAKKEIVPLVEEADLKSEFSMEVWRK
                     LGEMGILGLPFPEELGGSGASVETCCLAGEALGHAGVDQGHLLALGAHTYLCADTIFK
                     HGSPEQLKKYVPKLASGEWIGCMGLTEPGAGSD"
BASE COUNT          846 a         1000 c         1256 g          722 t
ORIGIN      
        1 ccggcggact gctttccggg catcccgtca tcgccgccgg cctctacagc atggccgcgg
       61 tggtccggca gattcgggga gacgcgggcg gttttcaggt gcaaaaagcg aaaaccggcc
      121 tggtgcaggg tgtcaacggg ctgggcggcc agtcccactg tgtgtggatt ctggacaagg
      181 acaagtagga ggacaaacca tggcgaaacg agttgcaatt gtaggaaccg gacagacctt
      241 ccataagagc catcgccccg acgtcaacgg acaggagctg atccatgagg cagttgcccg
      301 tgcgctggcg gacgccgacc tgaagctgaa ggacatcgac gccgtcgtca tcgggaacat
      361 ggatcatttt gaggggatca actacgtgga ctgctggagc gtggacggtt ccggcgggac
      421 gatgaagccc attatcaagc tgaccaccgg cggaaccacc ggctgcaccg tcgccatcgg
      481 cggatatcat ctggtcggct cgggaatgtt cgaccgagtg ctgatcatcg gctgggaaaa
      541 gaactccgaa tcggacacga ccggcgccat caccacggct tttgatccca tctgggaccg
      601 cctggtcttc gccggcgcaa tatcgggtct ggcggcggag gcgcaggctt acatggcccg
      661 ctacggcgcg acggaccgcg acggcgcccg ggtgagcgtg cgcgaccgga aacatgcgat
      721 gaacaatccg cacgcgcagc ttcgaaagga agtgagcctg gaagaagtgc tggcttcccc
      781 gatgctggcg gacccgatcc atcttttgga cgtttgcccc cgctccgacg gcgcgtgcgc
      841 ggtcatcatg gccagtgaag acatcgccga aaagatctgc ccgaaaccgg actggatctg
      901 gggcacggct gcgcgccact cctacagcta cctgggcgac gccgattacg gccgcctgac
      961 cagcatgcgc gaaggatcgc aggaactctg gaagaaggcg ggcatcaaag agccgcgcaa
     1021 agaactggat gttgtcgaga tgtaccagcc ctattcgttc ggcggtttga tctggatcga
     1081 ggacatgggc ctggtgggcc ccggcgaggc gccgcggtat atctgggacg gcaacaccga
     1141 catgggcggc gaactgccga tcaatccgtc gggcggcgtg atttcctgca atcccatcgg
     1201 ggcgaccggg ctgatccgct gcgcggaagc ggccatgcag gtgatgggca aagccgaagg
     1261 ccggcaggtt ccggacgtca actttgcctt cagtagcggt ttcggcggct gctggtggac
     1321 cgatatgatt ctgcacggaa agaaaaaacc ggcctgaagc aacaaggagg aaccagcatg
     1381 gcggaaaaag aaaacatcat tatcgaatcg ggcgacgcgg ttcagccctt cgcgtattcc
     1441 gtgggcatgc acggaagcag gttttttgcg gagattcgcg accataagcg gtttctgggg
     1501 atcaagtgcc ccaaatgcgg gaaggtgtat attccgccgc gcggcgtctg cggagactgc
     1561 tttgtcgaga tgaaagactg ggtcgaagtc gggccgaaag gggtcatcgg cacctttacg
     1621 attcttcgct acgcgtttat cgacccggaa acgggcgcgc agaagccggt cccctatggc
     1681 tacgggttta tcaggtttga cggcgccgac accctgttcc agcactacat caacattgag
     1741 gatgaagcga agattcgcgt gggcgcccgc gtggagccgg tatttgccgg agagcgaaag
     1801 ggcagcattc gggatattga gtattttcgg gtcatcgact gatggccgca aaggagtcgc
     1861 cgacggttga gatcaatcca ttcaagcgga ttctgaagat gccgggggct ctgtgcgggg
     1921 gaatcagcac cggcagcgat aaaatccgca gcggatacgg caacggggac tgcctttact
     1981 ttgattttga gcacctggtc ttcgccgtgg cggacggcac ggaacgcttc ccctgggcgt
     2041 cgagggatct tttgcagcgg ctgtcggaga ggcttttgcg gtccggttcc ccggaaacgg
     2101 cccgggattg gaaggacatg atgaacaccg gggtctatga cggccagaag taccagcata
     2161 agacgacctt cagcgccgtc gcccttcgcc gtgaaggaga ggatgttttt ctgatcatcg
     2221 cgaacggagg cgacagcgtc gtaacggtca tggatggcct tacgggaaag attcgccgtc
     2281 agacggggcg caacatggat tttgcgggaa gaagcaggca gattgtggat gtgacggagc
     2341 accggatgcc ggaccgggac caccgggtgc ttctttccac ggacgggttc gatgatgtct
     2401 ggcgcttcta tctgcgccgg tcgcccgccg gctccgcgcg cggcgttctc gaaagggttg
     2461 ggctggacgg aatcggcgag gtgatttccg ggcttctgga agagcaccgg gaccgttttg
     2521 agtatgatga tgtcggcttt attcttctgg atccgggcgc ggtgaagccg gtgaagggaa
     2581 aagctctgat catgggagga acgcggcctt ttgaagaaaa tcgctaccgg ctgcagtata
     2641 caccggaggc gcatgaccgg tggatccccg atgccgagtg ggccggacag gaggaaatgc
     2701 ttgacggcgc gggaatccgg gtgctgaaca cggtgcggtg ctgaaggacc ggattccctt
     2761 tcatgcaaga aagtgttgca gaagagatga atgagcggaa caaaaatgat ttgctgaatc
     2821 tgaggcggtc ccaactgacg gcggcggcgt acaaagtggt tgcgcagaag gggtacaacg
     2881 acttcaccat caaggatatt gccgaggaan nnnnnnnnng gaagccggcc tgtccacggg
     2941 gctggtccac tactacttca aaaacaagga agatcttctt ttcaagctgc tcaaggagat
     3001 gaacgccaat cttcgggaca atctgaaaaa ggccatgggg gagcttgccg aacccaggga
     3061 taaactgatg gctttctgcg acgaagcctt tgcgctggtg gaccgggaaa aggcgtattt
     3121 ttacgtgctc atcgattttt gggcgcagat caatcacgat gccaggattc gccaggcgaa
     3181 cgtcaaactt tttcagagct accgcgccga gatcgcggcg atcattcagg aaggggcggc
     3241 caaaggcgtt ttcaccgtgg aggacgtgaa cctgacgtcc gtgatcatca tcgcgctcat
     3301 tcagggaacc atcatccagt acgcgattga tcgcgaggcg ttcaattaca gcgagatcaa
     3361 ggagaaaatc aaggagcaga ttctctccag aatcgtcaac ggaaaataat gcattaaagg
     3421 aggcattcat ggaattcggt tttacggaag aacagctgat gttcagagat tcggtctata
     3481 agttcgcaaa aaaggaaatt gtgccgctgg tcgaggaggc agatctcaaa agcgagttct
     3541 ccatggaggt gtggcggaaa ctcggtgaga tgggcatcct gggccttccg tttcccgaag
     3601 aactgggcgg atcgggcgcc agtgtagaga cctgctgcct ggccggcgaa gccctgggcc
     3661 atgccggcgt cgatcaggga catctgcttg ccctgggcgc ccacacttac ctgtgcgcgg
     3721 acacgatctt caagcacggc tcgcccgaac agctgaaaaa gtacgttccg aagctggcca
     3781 gcggcgaatg gatcgggtgc atgggactga cggagccggg cgccggttcg gacg
//
LOCUS       DAOSIM010000196         3810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_21100_length_3810_cov_5.860719, whole genome shotgun sequence.
ACCESSION   DAOSIM010000196 DAOSIM010000000
VERSION     DAOSIM010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21100_length_3810_cov_5.860719"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..54
                     /gene="ssrA"
                     /locus_tag="PLR47_08345"
     tmRNA           <1..54
                     /gene="ssrA"
                     /locus_tag="PLR47_08345"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            262..2103
                     /locus_tag="PLR47_08350"
     CDS             262..2103
                     /locus_tag="PLR47_08350"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOG12995.1"
                     /translation="MSIIKLIEQKQKVGAFIVLLICILLSVASIITASLVQRDFGGVE
                     VSDVYFKNVNGVIVRAKLFVPDGATRDNPMPGMVYIHGYQNNRETGDAYSIETARRGI
                     VVLNIDAIGRGHSGLPGEPSDPGFDPTYGGKSAVAYLKSLVYVRTDAVGIMGHSLGAE
                     MAYLVALDDPTLAATVITGYGYTAEATPAMPRNMLMIIGRYDEFRGRMTGTRSIEKEW
                     MATKRTSKAFGIAHPELSTTYGDFSKGTARRVFVPRITHVQESHNACAITETVEWLKS
                     ALNPPPAKWIDARSQIWQVKEWATLVAMLAGFASLFPLALILIRLPFFAAIRRTRRDR
                     YECTMKDYTLYGSVNGIIMWLYLPLILVLFGIHKYLVPIHHAFPMMMVNAIVWWLLWV
                     NVIGLVLFRRWYKKRAQTQGVMLSDLGVSFSDAGFHLDRRALIKVFILSALLFGFMYA
                     VEFAAEKAFLVNYRFVYPFMNDLTPYRALMMLLYWPFVLFCFLVTGAFLHGQLRRPSD
                     NGWLTTFAARSAYAGLALCLPLILFLAVQYVPLFTTGFIPLVGPNGLFVAFVINLFPV
                     ILLFAITAALSTWLHQLTGTVYAGAILNSLIITWVFASSQVIAPIPV"
     gene            2166..2858
                     /gene="budA"
                     /locus_tag="PLR47_08355"
     CDS             2166..2858
                     /gene="budA"
                     /locus_tag="PLR47_08355"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HOG12996.1"
                     /translation="MLYISSPINALLEGFYRDDITFEMLKEKGDFGIGTFNNLDGEMI
                     ALNGSFFQLDLDGTAFPVDGKMKTPFAVLCHFKPMLTEEIIGPLSGATFMERLRHILP
                     SDNMFYAIHMAGSFASVETRSVPRTENYRPLAEATHDQKIRHFENIEGHLVGFYTPSF
                     VPSVSVPGYHFHFIDKTLSAGGHLLRCEPNHLDIQLQIFFSMELTLPKTLDYLTASFE
                     RNAREDLKKAER"
     gene            3064..>3810
                     /locus_tag="PLR47_08360"
     CDS             3064..>3810
                     /locus_tag="PLR47_08360"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOG12997.1"
                     /translation="MNDSNKKREHWATRAGFVLATAGSAVGLGNIWRFPYMTGEHGGA
                     AFILIYLVAVLLLGYPLVVNEMVLGRAAQRDPVGVFKVLAPNSPWWLVGALGVFTGFI
                     ILSYYAVVAGWSMAYIYKVVVATSVPGIDHARVFQDHIGSLWEPILWQALFLILTVGI
                     IAAGVVKGIQRWSMVLMPLLFVLLLILIVRSVTLPGAGEGLAYYLHPDFSKVTGRTLL
                     AALAQAFFSLSLGMGAIMTYGSYLDDKDEIP"
BASE COUNT          787 a         1024 c         1026 g          973 t
ORIGIN      
        1 gaagctccag cggaaggttt tcggacgcgg gttcaattcc cgccgcctcc accaagacaa
       61 aatccaacaa agtccaaaga agtgcaaaac ccgttagaaa taacgggttt tttgttgctt
      121 attgtccaaa gggggattat gtattcgaaa aaatggatgt ttggatcctg cgaactcaag
      181 aaagaaattg aaacaggtgc gaaaagccgg taggaatatc ggaatcaatg acattaatga
      241 tctatatgcc aggaggctgc aatgtccata attaaactta ttgaacaaaa acaaaaggtt
      301 ggcgcattta ttgttctgtt gatttgcatc ctgctgtcgg tggcatcgat catcacggct
      361 tctctcgttc agagagattt cggcggcgtg gaagtatcgg atgtctattt taaaaatgtc
      421 aacggcgtca tagtgcgggc aaagcttttt gtccccgatg gcgcaacgcg cgacaatccc
      481 atgcccggca tggtttacat tcacggctac cagaacaacc gcgaaacggg cgacgcctat
      541 tccatcgaga ccgcacgacg cgggatcgtg gtgctgaaca ttgatgcgat cggacgcgga
      601 cactccggcc tgccgggcga gccttccgat cccgggttcg atcctactta cggcggcaaa
      661 agcgcggtgg cctatcttaa atcgcttgtt tatgtccgaa cggacgcggt cgggatcatg
      721 ggacacagcc ttggcgcgga aatggcctac ctcgttgcac ttgacgaccc gacgcttgcg
      781 gcgaccgtta tcacgggata cggctacacg gcggaggcaa cgcccgccat gccgcgcaac
      841 atgcttatga tcatcggccg gtacgatgaa tttcgcggca ggatgaccgg gacgcggagt
      901 atcgaaaagg agtggatggc cacgaagcgg acatcgaagg cctttggcat tgcccatccg
      961 gaactatcca ccacgtatgg ggatttcagc aagggaaccg cccgtcgcgt gtttgtgccc
     1021 cgcattaccc acgtgcagga atcccacaac gcctgcgcca ttaccgaaac ggtcgaatgg
     1081 ctgaaatcgg cattgaatcc cccgcccgca aaatggatcg atgcccgcag ccagatctgg
     1141 caggttaaag agtgggcgac tctggttgcg atgcttgcag gattcgcgtc gctttttccg
     1201 ctggccctca tcctgatccg tcttcctttc tttgccgcga tacgccgcac ccgccgggac
     1261 cgctatgaat gcaccatgaa ggattacaca ctctatggat ccgtgaacgg catcatcatg
     1321 tggctctatc tgccgctgat tctggtgctc ttcggcatac acaaatacct tgtgccgata
     1381 caccatgcct tccccatgat gatggtcaac gccatcgtct ggtggctgct ctgggtcaac
     1441 gtcatcggac tggttctttt ccgacggtgg tataaaaaac gggcgcagac gcagggcgtc
     1501 atgctttcgg acctgggcgt ttctttttcc gacgccggct ttcatcttga ccgccgggcc
     1561 ctgataaaag tttttattct gtccgcgttg ctttttggtt ttatgtacgc cgtggagttt
     1621 gccgccgaaa aagcattcct cgtcaactac cgctttgtct atccgtttat gaacgacctg
     1681 acgccgtatc gcgccttgat gatgctgctc tactggccct ttgtcctgtt ttgtttcctt
     1741 gtaaccggcg cctttctcca cggccagcta cgacgcccct ccgataacgg atggctcacg
     1801 acgtttgcgg cgcgctcagc atacgccggt ctcgctttgt gcctgccgct gattctgttt
     1861 ctggctgttc agtatgttcc gttattcacc accgggttca ttccgttggt ggggcccaac
     1921 ggcctctttg tggcgtttgt catcaacctg tttcccgtca tacttctttt tgcgattacc
     1981 gcggcgctct cgacatggct gcaccagctg acgggcacgg tgtacgcggg cgcgatactc
     2041 aattcgctca tcatcacctg ggtgtttgct tcgtcgcagg tcatcgcccc cataccggta
     2101 taaaagagag ggcagggggc aatgatgagc gatccgctat ttcatttttt tgcttgaggc
     2161 cattcatgct ttacatcagc agccccatta atgcattgct ggaaggattt taccgggatg
     2221 atatcacgtt cgagatgttg aaagagaagg gggatttcgg catcggaacg ttcaacaacc
     2281 tggacgggga aatgatcgct ctcaacggtt cctttttcca gctcgacctt gacggaaccg
     2341 cctttcccgt ggacggaaaa atgaaaaccc cgtttgccgt tctttgccat ttcaagccga
     2401 tgcttaccga agaaatcatc gggccgttat ccggtgcgac attcatggag cgtctgcggc
     2461 atattcttcc gtccgacaat atgttttatg ccattcacat ggcaggttcg tttgcgtcgg
     2521 tcgagacgcg ctccgttccg cgaaccgaaa attaccgtcc gctggcggaa gcgacgcatg
     2581 accagaaaat ccgtcatttc gaaaatattg aaggccatct ggtcggattt tacacgcctt
     2641 cctttgttcc gtccgtcagt gtgccgggat accattttca cttcatcgac aagaccctgt
     2701 cggcgggcgg ccaccttctg cggtgcgaac caaaccatct ggacattcaa ctgcagattt
     2761 ttttcagcat ggagctgacg cttcccaaga cgctggatta tctcacggcg tcctttgaac
     2821 gaaacgcgcg ggaggatttg aaaaaagcgg aacgctgatt attgaagcaa ggtctggctt
     2881 cactcccaat gcttttcccg gttgatccgt tatttgatgc cggactttct ggtttcatga
     2941 aaacgaaccc ggcggcgtcc tcttctacgg tttttacctt gacactccgc gtggaaattc
     3001 ctatagttcg cggcgaaaaa acgtccgccg ccaatggaag cgcggggacc ggggacaaat
     3061 gagatgaatg actcaaataa aaagagggag cactgggcaa ccagggcagg attcgttctt
     3121 gccacggcgg gatcggccgt cggattggga aacatctggc gatttcccta catgacgggc
     3181 gaacatggcg gtgcggcctt catattaatc tatctggtgg ccgtcttgct tttgggttat
     3241 cccctggttg tcaatgaaat ggtcctgggt cgcgcggccc agcgggatcc cgtgggggtt
     3301 tttaaggttc ttgccccgaa cagcccctgg tggctggtgg gggcgctggg tgttttcacg
     3361 ggtttcatta ttctttccta ctatgccgtc gtcgccggct ggtcgatggc ctacatatac
     3421 aaggtagttg tcgccacgtc cgttccggga atcgatcatg cccgtgtttt tcaggatcat
     3481 atcggcagct tatgggagcc gattctctgg caggccctgt ttctgatcct gacggtcggc
     3541 attattgccg ccggtgtcgt taaaggcata cagcgatggt ccatggtttt gatgcctctt
     3601 ctcttcgtgc ttttgctgat tttaattgtc cggtcggtga cgctcccggg cgctggagag
     3661 ggtcttgcct actacctgca tccggatttc agcaaagtga cgggacggac gctgctggcc
     3721 gccctggcgc aggcattctt ttctctgagt ctgggaatgg gcgccatcat gacctatgga
     3781 agctatctgg acgacaagga tgaaattccg
//
LOCUS       DAOSIM010000197         3801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_21159_length_3801_cov_4.861986, whole genome shotgun sequence.
ACCESSION   DAOSIM010000197 DAOSIM010000000
VERSION     DAOSIM010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21159_length_3801_cov_4.861986"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PLR47_08365"
     CDS             complement(<1..838)
                     /locus_tag="PLR47_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOG12998.1"
                     /translation="MKKILRIDLSNRQYGFEDINSVYAGLGGRGFTSKVVSEEVSPKT
                     DPLGMANKLVFAPGILAGTAAPNNGRLSVGAKSPLTGGIKEANAGGAAAQKIARLGIR
                     GIVVEGFSSSLTMAKVTKDGVEFIPADEFKGMGNYEIIDRLRAGHGDGISVISIGPGG
                     EKLVKAAGISVTSPDFKIRMAARGGLGAVMGSKNLKALIIDDAGMKGVEVADAAALKK
                     AAADLTKGILSHPLTAGFRALGTPLLVNMINGSGCLPTRNYSMGQFKDAEKISGEYMA
                     ESQ"
     gene            complement(887..1180)
                     /locus_tag="PLR47_08370"
     CDS             complement(887..1180)
                     /locus_tag="PLR47_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG12999.1"
                     /translation="MMPVIDQIKEKQGQVTYPVKIKVRLFGLLSRYVPGYDHAKGIVV
                     EAQEEITYGDLITLLNLPESEIGFFSAGGIMKRHEEHIADGDEIKIFMPLAGG"
     gene            complement(1202..2020)
                     /locus_tag="PLR47_08375"
     CDS             complement(1202..2020)
                     /locus_tag="PLR47_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HOG13000.1"
                     /translation="MSVWNIKVLYYGELKIPKAMATPGLDADLLIDSPYLGFLLQNGK
                     RNVLVDTGISDNYIVDGKAFGNFPARGGLRYVEQSLEKAGVSPLEIDTILFTHLHNDH
                     AGNTSIFKNARLIFQKDEWANLLDPLPIVRVRKDYDLALIDELKAADCVKVHGDFELT
                     GGIKCFRTPGHTPGSMSVAVNTPNGIRILVGDLWPLTCLAFSRQTEITDMEGRKHRIT
                     PAPREYGHFMPPSSLICDFADYYDSCNRILAMIPEDSPEYIVAGHEPALVFTGV"
     gene            complement(2062..3360)
                     /locus_tag="PLR47_08380"
     CDS             complement(2062..3360)
                     /locus_tag="PLR47_08380"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG13001.1"
                     /translation="MKNATPEPSAEFKVSGFQRTSILVVCFLLYLINFMDRQVLSAVL
                     EPMKIDLGLTDAQAGILQTAFFLSMAVLGIPASYLVDRWSRRKTMSVVAIVWSVFTYV
                     TGLGRSFIGVLLPRIVVGVGEAGFPAASTAMLSSVYPQKSRGRILGIFNIAVPLGSAL
                     GALLGGYLSAHYGGWRTPFYVFAVPGVILGILAYFLKDYKTVIVVDEHGNKTGFGATI
                     VQLCKIPTIRWVYIGYAMHMSMLFSFAAWGGAFIMRAQNVSEAAAGMILGLSAITGII
                     GTPIGGVFADLWQKKNSKGRSFIPILSTSLAAVVLFLALMLDFKGPGFALVALYGVLA
                     VMAIPAVSSISQDVVSPGLKGVSWGMAGVVTMICGAAWAPSAVGIISDILGGGAYGLK
                     VALMCMCLCGFVSCFCFWRGSRHYPKDVEKVQGYVLECER"
     gene            complement(3385..>3801)
                     /locus_tag="PLR47_08385"
     CDS             complement(3385..>3801)
                     /locus_tag="PLR47_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13002.1"
                     /translation="MGTYFTGEPTREYRDMFELAASVHERVVRELKPGMKGSDVDRFV
                     EPFRKSGYMAASLVSGWCNYNSLPFVGQTNPDFPEKQRQNHLDYVFKPGLCVQVMAYP
                     IAPDFQKGLWVGSTCIFTEDGLRELNKYPVRELRVI"
BASE COUNT          906 a         1117 c          909 g          869 t
ORIGIN      
        1 cctggctttc cgccatgtac tcgccggaga tcttctccgc atccttaaac tgccccatcg
       61 aataattcct ggtgggaagg catcccgaac cattgatcat gttgacgagg aggggcgtcc
      121 ctaaagccct gaaccccgcg gtgagaggat gagataaaat gcccttcgtc aagtcggcgg
      181 cggctttttt gagcgctgcc gcatccgcca cttcaacgcc tttcattccg gcgtcatcga
      241 tgatcagcgc cttaagattt tttgaaccca ttaccgcccc cagaccgccc cgggccgcca
      301 ttctgatctt gaagtcgggg gaagtaacgg atatgccggc ggcctttaca agcttttcac
      361 cgccagggcc gatgctaatg acagatatcc cgtcaccgtg accggccctg agcctgtcga
      421 tgatttcata attccccatg cctttaaatt catcagccgg aataaactcg acgccgtctt
      481 tcgtcacctt cgccattgtc agcgatgaag aaaacccctc gacaacaatg ccccggatgc
      541 caagccgtgc gatcttctga gcggccgccc cgccggcatt ggcttccttg atgccgcccg
      601 tcagcgggct tttcgctccc acggaaagac ggccgttgtt gggcgcggca gtaccggcga
      661 ggattccggg cgcaaagaca agcttgttgg ccataccgag tggatccgtc tttggagaca
      721 cttcttcact gacgactttt gaagtaaacc cccgtccgcc cagaccggca tagacggagt
      781 ttatatcttc gaatccgtac tgcctgttgc tcagatcgat tcttaatatc tttttcatag
      841 tttgtgtttt cctccacaca ttgtcctgat gtactcccgc cccttctcat ccacccgcaa
      901 gaggcatgaa tattttgatt tcatctccgt ctgcgatatg ctcttcatgc cgcttcatga
      961 tgccgcctgc gctgaaaaaa cctatctcgc tttccggcag gtttaataat gtgatcaaat
     1021 ctccgtacgt aatctcttcc tgcgcctcga caacaatccc tttggcatgg tcataaccgg
     1081 gcacatacct tgaaagtaat ccgaaaagcc tgactttaat tttcacggga taagtcacct
     1141 gcccctgctt ttcttttatc tgatcaatca ccggcatcat gcatgaatcc cgacagcgat
     1201 tctaaacgcc ggtaaacaca agcgccggct cgtggccggc cactatgtac tccgggctgt
     1261 cttcaggaat cattgccaga atcctgttgc agctgtcgta gtaatcagca aaatcacaga
     1321 tgaggctgga cggcggcatg aagtggccgt attcgcgcgg cgccggagtg atcctgtgct
     1381 tcctgccctc catatccgta atttcggtct gccgggaaaa ggccaggcag gtcaaaggcc
     1441 agaggtcgcc gacaaggatc cggattccgt tgggcgtatt gaccgcaacg gacatggacc
     1501 caggcgtatg ccccggcgtt ctgaaacatt tgatgccccc ggtcaattcg aaatcgccgt
     1561 gaactttgac gcagtcggca gccttcaact cgtcgatcaa agccagatcg tagtctttgc
     1621 ggactctgac aatcggcagt ggatcgagca gattcgccca ttcgtctttt tgaaaaatca
     1681 gccgggcgtt tttgaatatc gacgtattcc cggcgtggtc attgtgcaga tgggtgaaaa
     1741 gaatcgtatc aatctcgagt ggactcaccc cggccttctc cagggattgt tcgacatatc
     1801 tgaggccgcc tctggccgga aaatttccaa aagccttccc gtcaacgatg tagttgtccg
     1861 aaatgccggt atccaccaga acattgcgct tgccgttttg cagaagaaat cccagatacg
     1921 gcgaatcaat gagcagatcc gcgtcaagac cgggtgtcgc catcgctttg ggaatcttga
     1981 gttctccata ataaagcacc ttgatattcc atacactcat atttactcct ccttcattta
     2041 caatcgtgtc tgccggcggt ttcaccgctc gcattcgaga acatatccct gaaccttttc
     2101 cacatccttc ggatagtgcc gggagcctct ccagaagcag aaacaggaca caaagccgca
     2161 caagcacatg cacatcaggg ctaccttaag gccgtaagcg ccgcctccca ggatatcgga
     2221 aataatcccc acagccgagg gcgcccaggc ggcgccgcaa atcatcgtga cgacaccggc
     2281 catcccccag gaaaccccct tgagtccggg actcaccacg tcctgagaaa tggagctcac
     2341 cgccggaatt gccataacgg ccaacacgcc atagagtgcg accagagcaa atcccgggcc
     2401 cttgaaatcg agcatcaggg ctaaaaacag cacaaccgcg gcaagggatg tagacaggat
     2461 agggatgaaa gatcgtcctt tgctgttctt tttctgccac aaatccgcga acacaccacc
     2521 aatcggtgta ccgataatac ccgttatggc ggagagcccc aagatcatac cggctgccgc
     2581 ctcagatacg ttctgggcgc gcatgatgaa cgcgccgccc caggcggcaa aagaaaaaag
     2641 catggacata tgcatggcat agccgatgta tacccacctg atggtaggga ttttacaaag
     2701 ctgaacaatc gtggccccga agccggtttt gttcccatgt tcgtcgacta cgatcacggt
     2761 tttgtagtct ttgagaaaat aggccagaat ccccagaatg acacccggta cggcaaaaac
     2821 ataaaaaggt gtccgccagc cgccataatg agcggataga tacccgccca gcaaagcccc
     2881 cagagcggag cccaagggaa cagcgatatt gaatattccc aggattctgc ctctggattt
     2941 ttgaggatat acgctgctca gcatcgccgt gctcgcggca ggaaatccgg cctcgccgac
     3001 cccaaccacg atccggggca aaagcacgcc gatgaagctc cggcccaggc cggtaacgta
     3061 agtaaagaca ctccagacaa tcgcaaccac agacatcgtc ttgcgcctgc tccagcgatc
     3121 caccagatag gaggcgggaa tccccagaac agccatactc aggaaaaagg ctgtttgcag
     3181 aattccggcc tgagcgtctg taaggcccag atctattttc atgggttcca gcaccgctga
     3241 aagcacctgt cgatccataa agttgatcag gtacagcagg aaacaaacca ccagtatact
     3301 ggttctttga aatccgctca ctttgaattc tgcagatggt tcaggtgtcg catttttcat
     3361 cggatcttct cctcgtaatt tcttttaaat cactcttaac tctctgaccg gatacttgtt
     3421 cagttccctc aatccatcct cggtaaatat gcaggtgctt cccacccaca gccctttctg
     3481 aaaatcgggc gcgatcggat aggccatcac ctgtacgcag aggcccggtt tgaagacgta
     3541 gtcgagatgg ttctgtcttt gcttttctgg aaaatcaggg tttgtctgcc cgacgaaagg
     3601 cagtgagttg taattgcacc agccggaaac caggcttgca gccatgtaac cggattttct
     3661 gaaaggctcg acaaacctgt ccacgtcgct tcctttcatg cccggcttga gctcacgaac
     3721 cacccgttca tgaaccgacg cagccagctc aaacatgtcg cggtattccc gggtcggctc
     3781 tcccgtaaaa taggtgccca t
//
LOCUS       DAOSIM010000198         3793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_21197_length_3793_cov_7.319422, whole genome shotgun sequence.
ACCESSION   DAOSIM010000198 DAOSIM010000000
VERSION     DAOSIM010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21197_length_3793_cov_7.319422"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1068)
                     /locus_tag="PLR47_08390"
     CDS             complement(46..1068)
                     /locus_tag="PLR47_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG13003.1"
                     /translation="MEKRLIKKDALAGIARKMAGDAMVWAPVKKEDNVLFAPLGTGDE
                     PVFTYLNSKNAPKNVFFPHTETLMKFTRTPKGMEFSSEVNQSGQSVLFGVRPCDAHSF
                     VLLDKLFDQEKYKDAYYIEKRKNTTVISLACVKPPYATCFCTSVDGEPVSADGADILL
                     TDLGGDYLVEFITDKGEKLASYFGDAKADAAADAKKAEIAAKARDAIQSKIPAHEIKP
                     ILDVNFDNPFWDTIHRKCLACGTCTFMCPTCHCFDISDEVKGSDGVRIRSWDSCMFPL
                     FTKETSGHNPRSSQKERWRQRTMHKFKYYIDMFNAISCVGCGRCVMACPVNIDIRKVV
                     ADISKL"
     gene            complement(1137..1985)
                     /locus_tag="PLR47_08395"
     CDS             complement(1137..1985)
                     /locus_tag="PLR47_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HOG13004.1"
                     /translation="MKVSYYPGCSLHAMGKEYDQSMKAVSRALNIELKEVDDWSCCGA
                     SAAHNTNFDLSIALPARNLIAAEKDAMDVMVPCAACFNRFKMAEHHLKSDKDLKARIE
                     GVLGARYQGGISIRNPIDVIYNEIGIDALAAKVVKPMTGLKPVSYYGCLLLRPPEVCQ
                     FENYENPFMLDKMMGALGADVKNWSYKTDCCGGSLSVSKTPIVVAMCDKLMAAAREAG
                     ANCVVTACPMCMANLEMRPSPNMKMPVFYFTELISLAMGLPGSKDTFNSHLTDPQPLL
                     NQLRLI"
     gene            complement(2057..2584)
                     /locus_tag="PLR47_08400"
     CDS             complement(2057..2584)
                     /locus_tag="PLR47_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG13005.1"
                     /translation="METFLEEVNKKIHGVPLQACYHCRKCSAGCPAASYMEYNPNKVI
                     KMIQNGQKDKLLSSSTIWLCLSCETCITRCPNQVDIARMMDVLRQMAIEEGVAAKEAN
                     VLKFHEAFLSSIKFGGRVHEMLMTVHYKLKSGDLFSDADLAPTMLLKGKLALLPPRTR
                     DLKSVRGIFEKTKSK"
     gene            complement(2608..>3634)
                     /locus_tag="PLR47_08405"
     CDS             complement(2608..>3634)
                     /locus_tag="PLR47_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG13006.1"
                     /translation="GEIITVENIETKLREEAKRLLLEKKAEVIIGYEKGTLPLTATPC
                     FITKPEEADKLVWNNLCSQNLAKFVHDLITQHKNSQKRVKPEDRKRKIIGVVARGCTT
                     RSMVIHLQERQYERDDVCIIGVPCDGYVDGKGLKVKIDGADIMDGAVEGGQIKMKTSD
                     GDKTVVLKDVLADCCKTCRFNNPIISDVMIGSPAPPMNPDAEYDEVNAFEAKAMEERW
                     AYFTKEMEKCMRCNACRQACPSCYCPVCFVEQSQPQWVGIGEDATDTQVFQFMRLYHM
                     VGRCVDCGSCVAVCPMGVDLRKFLKKLDKDCFEFFGNRAGSGMEDMPPLGKFDEHHDK
                     GDFVYNP"
     assembly_gap    3635..3721
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          771 a         1040 c          977 g          918 t
ORIGIN      
        1 caacgggata aggaatatac gggttttgca ttacgaactc cctgattata atttagaaat
       61 atctgcaacc actttacgga tatcaatgtt tacgggacaa gccatgacgc accggccgca
      121 gcccacgcag gaaatcgcat tgaacatgtc aatgtaatac ttgaacttgt gcatggtccg
      181 ctggcgccat ctctccttct gcgaagaacg cggattgtgt ccggacgttt cctttgtgaa
      241 gagcggaaac atgcaggaat cccaagaccg gatgcgcacg ccgtccgatc ccttgacctc
      301 gtcgctgata tcgaagcagt ggcaggtggg gcacataaac gtgcaggttc cgcaggccag
      361 gcacttgcga tgaatggtgt cccagaaagg attgtcgaaa ttgacgtcga gaatcggttt
      421 gatttcatgc gccggaattt tggactggat cgcatccctg gctttggcgg caatctccgc
      481 tttcttggca tcggcggccg cgtcggcttt ggcgtcgccg aaataggagg cgagcttctc
      541 gcccttatcg gtgatgaatt ccaccagata atcgcctccg aggtcggtga gcagtatgtc
      601 cgcgccgtcg gctgaaaccg gctctccgtc caccgacgtg cagaaacagg ttgcgtaagg
      661 cggcttcacg caggccagcg agatcaccgt cgtgtttttc cgtttttcaa tatagtacgc
      721 gtccttgtat ttttcctgat cgaaaagctt gtccagcaga acaaagctgt gggcgtcgca
      781 ggggcgcacg ccgaacaaaa ccgactgccc ggattgattg acctccgacg aaaattccat
      841 gcctttcggc gtgcgggtga acttcatcag ggtctccgtg tgagggaaga aaacattttt
      901 cggcgcgttt ttggaattca gataggtgaa aaccggctcg tcgcccgttc cgaggggtgc
      961 aaaaagaacg ttgtcttctt tctttaccgg cgcccaaacc atcgcatctc cggccatttt
     1021 cctggcaata cccgccagcg catctttctt gatcaaacgt ttttccataa ttatccctaa
     1081 ttaatttttt atccagctta tcagtttcaa agcgtcgggt gttgtttcct caccgatcaa
     1141 atcaaacgca gttgattcag aagcggctga ggatcggtca ggtgcgaatt gaaggtatcc
     1201 ttcgaacccg ggagccccat cgccagcgaa atcaattccg taaaatagaa caccggcatc
     1261 ttcatgttcg gactggggcg catttccagg ttggccatgc acatcggaca tgccgtgacc
     1321 acacagttgg cgcccgcttc tctcgccgca gccatcagct tgtcgcacat cgccaccacg
     1381 atgggggttt tgctgaccga gagcgaaccg ccgcagcagt ccgttttata cgaccagttc
     1441 ttcacgtccg cccccagcgc tcccatcatt ttgtcgagca tgaaggggtt ttcgtaattt
     1501 tcaaactggc agacttccgg cggacgaagc agcagacagc cgtaatagga cacgggcttg
     1561 aggccggtca tgggttttac gactttcgcg gccagtgcgt cgatgccgat ttcattgtaa
     1621 atcacgtcaa tcgggttgcg gatggagatg ccgccctgat acctggcgcc cagcactcct
     1681 tcgatgcggg ctttgaggtc cttgtcggac ttcaggtgat gctccgccat tttgaagcgg
     1741 ttgaaacagg cggcgcaggg aaccatgaca tccattgcgt ccttttccgc ggcaatcaga
     1801 ttgcgcgccg gcagggcgat ggacagatcg aaattcgtgt tgtgcgcggc cgacgctccg
     1861 cagcaggacc aatcgtcgac ttctttgagt tcgatgttga gggctcggct gacggccttc
     1921 atggactgat cgtattcttt tcccatcgcg tgcagtgagc aacccggata atatgaaact
     1981 ttcaagagag tagcctcctt ttaaactgtt acttcagcgc gccctggccg ccgggggcct
     2041 ttcgcgcgat attccatcat ttgctctttg ttttctcaaa aatgcctcga accgatttca
     2101 gatcccgcgt cctgggcggc agaagcgcca gtttcccctt caggagcatg gtcggggcaa
     2161 ggtccgcatc cgaaaagagg tctccggatt taagtttgta atgaaccgtc atcagcattt
     2221 catggacgcg gccgccgaac ttgatgctgg acaggaacgc ctcgtggaat ttgagaacgt
     2281 tggcctcttt tgcggcaacc ccttcttcaa tcgccatctg gcggagaacg tccatcatcc
     2341 gcgcaatgtc cacctggttg gggcagcggg taatgcaggt ctcacacgac aggcaaagcc
     2401 agatcgtcga actggacagc agcttgtcct tctggccgtt ctggatcatc ttgatcacct
     2461 tgttggggtt atattccatg taggacgccg cgggacatcc ggccgagcat ttccggcagt
     2521 gatagcaggc ctgcagcgga acgccgtgta tttttttgtt tacttcttcc agaaaagttt
     2581 ccatgtaaac tccatcatct cattcaatta cgggttgtag acaaaatcgc ctttatcgtg
     2641 atgttcatcg aatttcccca ggggcggcat gtcttccatg ccggacccgg cccggttgcc
     2701 gaaaaattca aagcaatcct tgtcgagttt cttgaggaat ttgcgcagat ccacgcccat
     2761 cggacagacg gcgacgcagg aaccgcagtc cacgcagcgt ccgaccatgt gatacagacg
     2821 catgaactgg aacacctgcg tatcggtcgc gtcctcgcca atgccaaccc actgcggctg
     2881 gctctgctcc acaaagcaaa ccggacagta gcaggaggga caggcctggc ggcaggcgtt
     2941 gcaacgcatg cacttttcca tttccttggt gaaatacgcc cagcgttctt ccatggcctt
     3001 ggcttcgaac gcgttgactt cgtcgtattc cgcatccggg ttcatgggcg gcgcgggcga
     3061 tccgatcatc acgtcggaaa taatcggatt gttgaagcgg caggtcttgc agcagtccgc
     3121 cagcacgtct ttgagcacga cggtcttgtc gccgtcggac gttttcatct tgatctggcc
     3181 gccctcgacg gcgccgtcca tgatatccgc gccgtcaatt ttcactttca gtcctttgcc
     3241 gtccacgtag ccgtcgcagg gaacgccgat gatgcagacg tcgtcgcgtt catactgtct
     3301 ttcctgcaga tggatgacca tggagcgcgt cgtgcagccg cgggcaacca caccgatgat
     3361 tttcctcttc cggtcttccg gtttcacgcg cttctgtgaa tttttgtgct gcgtgatcag
     3421 atcgtgcaca aacttggcca ggttctgtga gcagaggttg ttccagacca gtttgtcggc
     3481 ctcttccggc ttcgtgatga aacaaggcgt cgccgtcaac ggaagcgttc ctttttcata
     3541 gccgataatc acctcggcct tcttttccaa aagcagcctc tttgcttctt cccgcaattt
     3601 tgtttcgatg ttttccacag tgatgatttc cccgnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nccgcgccac cagatttcct gagatatagt ggcattcacc cgggtgtcaa cccgaaacca
     3781 gaacgccgtc ggc
//
LOCUS       DAOSIM010000199         3781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_21287_length_3781_cov_6.560655, whole genome shotgun sequence.
ACCESSION   DAOSIM010000199 DAOSIM010000000
VERSION     DAOSIM010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21287_length_3781_cov_6.560655"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLR47_08410"
     CDS             complement(<1..253)
                     /locus_tag="PLR47_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HOG13007.1"
                     /translation="MKVVYSEEYKQVYSYDPASNPGRIESIEFALAGKVDYVEPRPAT
                     EEEIRAVHTPNHIAQVRREGVYDIAALAAGGAIRAAEIGR"
     gene            complement(268..1041)
                     /locus_tag="PLR47_08415"
     CDS             complement(268..1041)
                     /locus_tag="PLR47_08415"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HOG13008.1"
                     /translation="MCLILFAYHVHPAYRLILAANRDEFYDRPSAPADFWNSHPQMLA
                     GMDLKEKGTWLGVTKEGKFAAITNYRDPASWKADAPSRGKLVSRYLTGSAGPEKYLRN
                     VVKKADIYNGFNLLLGDGADLFVYSNRGNIQKLSPGIYGLSNELLDAPWPKVRRGKKL
                     LKKALAQKGTELEEALFELLSDRAAPPDRNLPDTGIGLEWERLLSTMFIKSPVYGTRS
                     STLLFIGKNRRVKLIEKIYNGEEEPWLVSRFAFPVEGRT"
     gene            complement(1123..1887)
                     /locus_tag="PLR47_08420"
     CDS             complement(1123..1887)
                     /locus_tag="PLR47_08420"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOG13009.1"
                     /translation="MDQKIDQVAKLAAKAGKIAVFTGAGISTESGIPDFRGPGGLWTK
                     YDPDDFTIDKFLGSHATRKKMWQRLREGGLMEDAQPNAAHYAVVELEKMGKLLSLVTQ
                     NVDNLHQKAGSSPALIRELHGNMQRLVCLNCRESYPIALVKEKYAGSDDVPTCDYCRG
                     ILKPDVIFFGEALPQQTLAQAMREAEDCDLMIVIGSSLVVYPAAYVPVHARQAGADLV
                     IINKGETEQDHIADVRIDAPAGEVMTKILNRLKAIG"
     gene            complement(1985..2758)
                     /locus_tag="PLR47_08425"
     CDS             complement(1985..2758)
                     /locus_tag="PLR47_08425"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOG13010.1"
                     /translation="MKKMAAGIIGGTHGMGRWLAGLLREQGFAVRVAGRETKTTAADL
                     ADRCDVVVVAVPIAATADVIREVGPKVKKGRLLMDLTSLKAEPVALMLAHSEADVVGC
                     HPLFGPSVNEPAGHPIVLCRGRGDDGHVLMKDVFGKAGYPVLETTPEAHDKMMSIIQA
                     LNHFNTIALGMAVAEAGIPFSELQPFSTPMFKTKMEMVQKVFTETPGLYADILAGNPR
                     TKKIMASYARVVRKIRALMDAGDAAGLKKMMEDTAKTLF"
     gene            complement(2755..>3781)
                     /gene="aroC"
                     /locus_tag="PLR47_08430"
     CDS             complement(2755..>3781)
                     /gene="aroC"
                     /locus_tag="PLR47_08430"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOG13011.1"
                     /translation="SHGPALGAVIDGCPAGIELAVEEIQRELDRRRPSASAASTSRAE
                     KDRVEILSGVFEGQTTGTPISLLVANADAQSSHYEDLKDVFRPGHGDYTYWKKYGMRD
                     WRGGGRASGRETVARVAAGAVARKVVRAAGMEVTAYTHSIGGIEISSKALKQDPHWLE
                     KVRENSLYCPDSQASAAMEERLSAVRKEGDTLGGVVEILVRGCPAGLGEPVFDKMDAD
                     LAKALMSIGTVKAVEVGDGCEVASKKGSEVNDPMDAEGFVKNSAGGILAGITTGQDIV
                     FRVYCKPIPSIALPQQTVDESGRQRSIQVVGRHDVCVIPRIVPVCEAMVLLALADHWL
                     RRKAGVR"
BASE COUNT          759 a         1210 c         1006 g          806 t
ORIGIN      
        1 accgtccgat ttccgcggcc ctgatcgccc cgcccgcggc aagcgcggcg atgtcgtaaa
       61 cgccctcgcg ccggacctgt gcgatgtgat tcggcgtgtg cacggcgcgg atctcttctt
      121 ccgtggcggg ccgcggctcc acgtagtcca ctttccccgc cagcgcgaat tcgatggatt
      181 cgatccttcc ggggttggat gcggggtcat acgagtaaac ctgtttgtat tcctccgaat
      241 aaaccacctt catggggcga cctcctttca tgtccggccc tccacgggaa acgcaaaccg
      301 gctgaccagc cagggctctt cctcgccgtt gtaaattttt tctatcagtt taacccgcct
      361 gtttttcccg atgaagagca gcgttgaaga gcgcgtgccg taaacggggc ttttgataaa
      421 catcgtcgag agcagcctct cccactcgag gccgatgccc gtgtccggca gattccggtc
      481 gggtggcgcg gcccggtccg aaagaagctc aaacagcgcc tcttccagtt ctgttccttt
      541 ctgggccagg gctttcttca ataatttttt gccgcgccga accttgggcc agggcgcatc
      601 gaggagctcg ttgctcaagc cgtaaatccc gggagaaagt ttttggatgt tcccccggtt
      661 ggaatacaca aagaggtccg caccgtcgcc caaaagaagg ttaaacccat tgtaaatgtc
      721 ggcttttttg acaacattcc gtagatattt ttcggggccg gcagatccgg tcagataccg
      781 gctgaccagc ttgccgcggg acggcgcgtc cgctttccag gaagcgggat cgcgataatt
      841 ggtaatggcg gcgaatttgc cttctttcgt gacgcccagc catgttcctt tttcttttaa
      901 atccatcccc gccagcatct gcggatgact gttccagaaa tccgccggcg cggaaggccg
      961 gtcgtagaat tcatcgcggt tggccgccag gatcaggcgg tatgcgggat gcacatgata
     1021 ggcaaaaagg atcaggcaca taagaatcct caggcggtta gactgaaggc tgaagactga
     1081 aggtcaaaag gaacgggcgc acagcgcccc tcaaaacagc ctttagccta tcgcctttag
     1141 cctattcaat attttcgtca tgacctcccc ggccggggcg tcaatgcgaa cgtcggcgat
     1201 gtgatcctgt tcggtttcgc ccttattgat gatcaccaga tcagcccccg cctgcctggc
     1261 atgaaccgga acataggcgg cggggtaaac caccagcgac gagccgatca cgatcatcaa
     1321 atcgcagtct tccgcttccc gcatcgcctg agccagcgtc tgctgaggca gggcctcccc
     1381 gaagaaaatc acatcgggct tgaggatgcc ccggcagtaa tcgcaggtcg gcacatcgtc
     1441 ggaaccggca tatttttcct tgaccagggc aatcgggtag ctctcacggc agttcaggca
     1501 aaccagcctc tgcatgttgc cgtgcaattc ccggatcagc gcgggcgaac tccccgcctt
     1561 ctgatgcaga ttgtccacat tctgcgtgac taaagacagc agtttgccca tcttctccag
     1621 ctccaccacg gcataatgag ccgcgttggg ctgcgcgtct tccatcaggc cgccttcccg
     1681 caggcgctgc cacatttttt tgcgagttgc gtgagatccc agaaatttat cgatggtgaa
     1741 atcatccgga tcgtacttgg tccagagccc tccggggccg cgaaaatcag gaatgccgga
     1801 ttccgtgctg atgcccgcgc cggtgaaaac ggcaattttt ccggctttcg ccgccagctt
     1861 cgcaacttga tcgatttttt gatccataaa agacgggcgc tcctttgatt aaaacgttat
     1921 gcagtgtgaa caaaatacac catctgtctc aaaaatcaaa agaaaatctc ccgtccggac
     1981 cgtctcagaa cagcgttttt gccgtatcct ccatcatctt cttgaggccg gcggcgtcgc
     2041 ctgcgtccat cagggcgcga atcttccgca cgacgcgtgc gtaggacgcc atgatctttt
     2101 tcgtgcgggg attcccggca agaatgtctg cgtaaagccc cggcgtttcg gtaaacacct
     2161 tttgcaccat ctccattttg gttttaaaca tcggcgtgga aaacggctgc agctccgaaa
     2221 aaggaattcc agcttcggca accgccatgc ccaacgcgat ggtattgaaa tggttgaggg
     2281 cctgaatgat cgacatcatc ttgtcatgcg cctcgggcgt tgtctccaga accggataac
     2341 cggcttttcc aaaaacgtcc ttcatcaaaa catggccgtc atcgccccgc ccccggcaca
     2401 gaacgatggg atgtccggca ggctcgttga ccgacgggcc gaacagcgga tggcagccga
     2461 cgacatccgc ctcggaatga gcgagcatca gcgccaccgg ctccgctttc agcgacgtca
     2521 aatccatcag cagccggcct ttcttcactt tcggtccgac ttcccgaatg acgtccgccg
     2581 tcgccgcaat cggaacggca accaccacca cgtcgcaccg gtcggccaga tccgccgccg
     2641 tggttttggt ttctctgccc gccacgcgaa cggcaaagcc ctgctcacga agcagaccgg
     2701 ccagccagcg gcccatgccg tgcgtgccgc cgataatgcc cgccgccatt ttcttcatcg
     2761 gacacccgct ttccgccgaa gccagtggtc cgccagcgcc agcagaacca tcgcttcgca
     2821 aaccggaacg atcctcggaa tgacgcaaac atcgtgtctg ccgacgacct gaatcgaacg
     2881 ctgccggccg ctttcgtcca ccgtctgctg cggcagtgcg atggagggga tgggtttgca
     2941 gtacacccgg aagacaatgt cctggccggt ggtgatgccc gcgaggattc cgcccgcgga
     3001 atttttgaca aagccttcgg catccatcgg atcgttgacc tcggaccctt tttttgacgc
     3061 gacttcacag ccgtctccca cttccacggc cttcaccgtt ccgatgctca tcagggcttt
     3121 ggccagatcc gcgtccatct tgtcaaaaac cggctccccc aaaccggccg ggcatccccg
     3181 gacaagaatc tccacaacgc ctcccagcgt atccccttcc ttccgaacgg cggacagtcg
     3241 ctcctccatt gccgccgacg cctgcgaatc cggacaatac agggagttct cccgaacttt
     3301 ttccagccag tgcggatcct gcttcagagc cttcgaagaa atctcaatgc cgccgatgct
     3361 gtgagtatag gcggtcactt ccatgccggc ggcccggacc actttccggg ccacggcccc
     3421 cgccgcaacg cgggcgacgg tttcgcgccc cgaagcacga cctcctcccc gccagtcccg
     3481 catgccgtat tttttccagt acgtgtagtc cccgtggccg ggacgaaata cgtctttcaa
     3541 atcctcgtag tgcgaagatt gcgcgtcggc atttgccacc agaagagaaa tcggcgtgcc
     3601 ggtggtttga ccctcgaaaa cgccggacaa aatttccacc cggtcttttt cggcgcggga
     3661 cgtggaagcc gctgacgcgg aaggcctgcg gcgatccagc tcccgctgaa tctcttcgac
     3721 agccagttcg atcccggccg gacagccgtc aatcaccgcg cccagcgctg ggccgtgcga
     3781 c
//
LOCUS       DAOSIM010000200         3761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_21436_length_3761_cov_7.287642, whole genome shotgun sequence.
ACCESSION   DAOSIM010000200 DAOSIM010000000
VERSION     DAOSIM010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21436_length_3761_cov_7.287642"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..>1192
                     /locus_tag="PLR47_08435"
     CDS             107..>1192
                     /locus_tag="PLR47_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HOG13012.1"
                     /translation="MKDALEAELKKQNMADVDVLATGCLGFCEKGPMMIIHPDKYFYQ
                     SVKESDIPEIVEKTIGKGEIISRLLYRHPETQKTLFREEDLPFYKKQLRAVFGKNGMI
                     DPSRIEDYFAIDGYQALAKVLAEMTPEQVISEVKKAGLRGRGGGGFHAAVKWKGVRNA
                     AGEPKYVIANGDEGDPGAYMDRSLMEGNPHSVIEGMIIGAYAVGSNHGYIYVRNEYPL
                     AVENLSKAIEAAKANGLLGKNILGTDFSFDIQINKGAGAFVCGESSALFASIEGRAGE
                     PRAKYVHAVDKGLYDKPTVLNNVETWANVPLIINKGADWFASIGTEGSKGTKIFSLVG
                     KINNTGLVEVPMGTTLYEIIYDMGGGIP"
     assembly_gap    1193..1202
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1203..1818
                     /locus_tag="PLR47_08440"
     CDS             <1203..1818
                     /locus_tag="PLR47_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOG13013.1"
                     /translation="VQTGGPSGGCIPESLLDIPVDFDKLYEVGSMMGSGGMIVMDETS
                     CMVDVARYFLEFLKEESCGKCVPCREGVRRMSDILEDICAGKGREGDVEILEAMGKAI
                     ADGSLCALGGSAPNPVLSTIRYFREEYDAHIREHRCPAGVCKALIAYSIDAEKCTGCG
                     VCVKVCATQAITGEKKKAHSIDKDKCTRCGACIQSCKFDAIKVK"
     gene            1833..2546
                     /locus_tag="PLR47_08445"
     CDS             1833..2546
                     /locus_tag="PLR47_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOG13014.1"
                     /translation="MVNMMIDGKKVSAPKGAMLLESIRGAGISVPTLCAHEAISRSGA
                     CRLCVVEIRKGNRTRIVTSCLYGVEEGLIVDTKSERVLNVRRLVMELLLSRCPESDVL
                     QKMARDMGVEPQPRYAADQDKGKCILCRSCVRVCEEVVGVSAIGLFSRGSCKEVGTPY
                     NEKSEVCIGCGACAYVCPTGHIEMTSTGDKRRIWGRSFKMQACSKCGKFFAPVDQLKF
                     ISKKTGASMEELSVCTECR"
     gene            2687..3382
                     /locus_tag="PLR47_08450"
     CDS             2687..3382
                     /locus_tag="PLR47_08450"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG13015.1"
                     /translation="MKDSGHGVIRLLTGMRDARLVWDTLGFLYNQRIYPLIAGLYESI
                     AEELKVQDGARILEAGAGRGYLSLLLASRNPKARFTGIDFSRMQVRRAERYRLQKKIF
                     NCSFQQNDVQSLPFPEAAFDAAVSVGSIKHWSDSRRGLSELRRVIKPGGCLMIAETNR
                     KTPDDELREFGKRFPVPFVPRGLLFWGLRRVVFGQSLSEDQLADAAGRAGFYPVERRP
                     VTTCPYVMVKAWK"
     gene            3509..>3761
                     /locus_tag="PLR47_08455"
     CDS             3509..>3761
                     /locus_tag="PLR47_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOG13016.1"
                     /translation="MKRNPLIHRRLAAARLPVMLFFLAALLAACSMFSAKPVQPAKIA
                     VVLGAGASKGFAHIGVLKVLEAQQIPVHMVVGTSAGSIVG"
BASE COUNT          842 a         1017 c         1131 g          761 t
ORIGIN      
        1 acaaagccac gcaatactgg acaacttgac gctgtattac ggtccgaaaa gaaagtttgt
       61 cgcaatttgt gccggtacag gctgccgcgc ttacggcagt cttaaagtga aagacgccct
      121 ggaagcggag ttgaaaaaac aaaatatggc ggatgtggac gtcctggcca ccggctgcct
      181 ggggttttgt gaaaaagggc cgatgatgat catccatccc gacaaatatt tttatcagtc
      241 ggtcaaggaa agcgacattc cggaaatagt tgaaaaaacc atcggcaaag gcgaaatcat
      301 cagccgtctt ttataccgtc atccggaaac ccaaaagacc cttttccggg aagaggacct
      361 tcctttctac aaaaaacagc ttcgtgcagt cttcggcaaa aacggcatga tcgacccgtc
      421 gcgcatcgag gattattttg ccatcgacgg ttatcaggcg ctggccaaag tccttgccga
      481 aatgacgccc gagcaggtga tcagtgaagt gaagaaagcc ggtctgcgcg gccgcggcgg
      541 cggcggcttt cacgcagccg tcaaatggaa gggcgtccgc aatgcggcgg gagagcccaa
      601 atatgtgatt gccaatggtg acgaagggga cccgggtgcg tacatggacc gcagtctcat
      661 ggaaggtaac ccgcacagtg taatcgaggg catgattatc ggcgcctacg ccgtcgggtc
      721 gaatcacggc tatatctatg tccgcaacga atacccgctg gccgtggaaa acctttcgaa
      781 agccattgaa gcggcaaaag caaacgggct tttaggaaaa aatattctgg gcactgattt
      841 ttcgtttgat atccagatca acaaaggggc gggcgcgttt gtctgcggcg aatcttcggc
      901 gctttttgcc tcgattgaag gtcgtgccgg cgagccgcgc gccaaatacg ttcatgcggt
      961 cgacaaaggg ctttacgaca aaccgacagt gctgaacaac gtcgagacct gggccaacgt
     1021 gccgctcatc atcaataagg gtgcggactg gttcgcctcc atcggaacgg aaggctccaa
     1081 ggggaccaaa attttctcgc tggtcggcaa aataaacaat acaggccttg ttgaagtccc
     1141 gatgggcacg acgctctacg aaatcatcta cgacatgggc ggcggcattc ccnnnnnnnn
     1201 nncgtccaga ccggcggccc ctccggcggc tgcattccgg agagcctgct ggatattccc
     1261 gtggattttg acaagcttta cgaagtcggg tcgatgatgg gctccggcgg catgatcgtc
     1321 atggacgaaa cgtcctgcat ggtggacgtt gcccggtatt tcctggagtt tctgaaggaa
     1381 gaatcctgcg gcaagtgcgt gccctgccgc gaaggcgttc gccggatgag cgacattctg
     1441 gaggacatct gcgcgggaaa aggcagggaa ggagacgtgg agattctgga ggccatgggg
     1501 aaggcgatag ccgacggctc gctttgcgcc ctgggcggct ccgcccccaa cccggtttta
     1561 agcacgatcc ggtatttccg cgaagagtat gacgcgcaca ttcgcgagca ccggtgtccg
     1621 gcgggcgtct gcaaggcgct cattgcctac agcatcgacg cggaaaaatg caccggctgc
     1681 ggcgtctgcg tcaaggtctg cgccacgcag gccatcaccg gcgaaaagaa gaaggcccac
     1741 agcattgata aagacaagtg cacccgctgc ggcgcgtgca tccagagctg caaattcgac
     1801 gcaatcaaag tgaagtaaag gtggagcaag ccatggtaaa tatgatgata gacggaaaaa
     1861 aagtgtcggc gcccaaaggg gcgatgcttc tggaatccat acgcggcgcg ggcatttcag
     1921 tccccactct ctgcgcgcat gaagcgatct cacggtccgg agcgtgccgc ctgtgcgtgg
     1981 tggaaatcag gaagggaaac cggacgcgca tcgtcacctc ctgcctctac ggcgtggaag
     2041 aagggctgat tgttgacaca aaaagtgagc gggttctcaa tgtccgccgg ctggtcatgg
     2101 aattgcttct ttcgcgctgc ccggaatccg acgtactgca aaagatggcc cgggacatgg
     2161 gtgtggagcc gcagccccgg tatgccgcag accaggataa gggcaagtgc attttgtgcc
     2221 gctcctgcgt gcgcgtctgc gaggaagtgg tcggcgtgag cgccatcggg ttgttttccc
     2281 ggggttcctg caaggaagtc ggcacgccgt ataacgagaa atcggaagtc tgcatcggct
     2341 gcggcgcgtg cgcctatgtc tgccccaccg ggcatatcga aatgacctcg accggcgaca
     2401 agcgcaggat atgggggcgt tcctttaaaa tgcaggcctg ctccaagtgc ggcaaatttt
     2461 tcgctcccgt ggaccagttg aaattcatca gcaaaaaaac cggcgcgtcc atggaagagc
     2521 tgtccgtctg tacggaatgc cgctgaaccg ttgcctgttg cgcctcgcct gtgaaaaatt
     2581 gagcccggtc ctgaaaatat caggccgggc tttttaaaga aacaaagcat ttatgaagac
     2641 agtcccagaa catcgcgcaa cgattcgccc ggaagaaaac ggatgcatga aggattcggg
     2701 tcacggggtg atccggcttc tgaccgggat gcgggacgct cgcctcgtct gggacactct
     2761 gggtttcctt tacaaccagc ggatttaccc gctgatcgcc gggctttacg aatccatcgc
     2821 ggaagaactg aaggtgcaag acggggcgcg gattctcgag gcgggcgcgg gccggggcta
     2881 cctgtcgctg ctgctggcgt cgagaaatcc caaagcccgt tttacgggaa tcgacttttc
     2941 ccgaatgcag gttcgccggg ccgagcgcta ccgcctgcag aaaaaaattt tcaactgttc
     3001 ttttcagcaa aacgatgttc agtccctgcc gtttccggaa gcggcctttg acgcggcggt
     3061 cagcgtggga tcgatcaagc actggtccga ttcccgccgg gggctttcgg agctgcggcg
     3121 ggtcatcaag ccgggcggct gcctgatgat cgcggaaacg aaccgcaaga cgccggatga
     3181 cgaactgcgg gaattcggca aacgcttccc agttcctttt gtgccccggg gccttctttt
     3241 ctggggactg cgccgcgtcg tcttcgggca gagcctgtcg gaagatcagc ttgcggacgc
     3301 cgcaggccgg gccggctttt acccggtcga gcgacggccc gtgaccactt gcccctacgt
     3361 catggtaaaa gcctggaaat aaccggcaag gggctttctg atttcctttc ttcgaacatc
     3421 ccgttgccat ttctcaccgg ctgcagtata acatcacgcg gggtttgatg aaaaatttcc
     3481 ggttgaggaa tccaaaaggg aaaatcatat gaaacgcaat cctctgatcc atcgtcggct
     3541 cgcggcggcc cgccttccgg tgatgttgtt ttttctggcg gcgcttctgg ccgcctgctc
     3601 gatgttctcc gcaaaaccgg tccagccggc caaaatcgcc gtcgtcctgg gggccggagc
     3661 ctccaagggc ttcgcgcata tcggcgtttt gaaggttttg gaggctcagc aaattcccgt
     3721 tcacatggtg gtgggcacca gcgcgggcag catcgtggga a
//
LOCUS       DAOSIM010000201         3755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_21490_length_3755_cov_7.704865, whole genome shotgun sequence.
ACCESSION   DAOSIM010000201 DAOSIM010000000
VERSION     DAOSIM010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21490_length_3755_cov_7.704865"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLR47_08460"
     CDS             complement(<1..269)
                     /locus_tag="PLR47_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HOG13017.1"
                     /translation="MSVIQEVIEKVKQTDPDQPEFHQAVAEVMETLEPTVKKHPEFVK
                     ASIYERIVEPERAVIFRVPWMDDQGKVRVNRGFRVQFNNAIGPFKG"
     gene            complement(303..1304)
                     /locus_tag="PLR47_08465"
     CDS             complement(303..1304)
                     /locus_tag="PLR47_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HOG13018.1"
                     /translation="MKELFKPEDIERKVLLILKILHESPDPMGARVIARRMSDYHVQL
                     SERTVRYHLKIMDERGLTKLVGRRDGRVITEQGFAEISDARVQDKIGLSISRIDLLSF
                     RTTFDVKKKRGLVPVNVSFFPKEHFSRALSIMKPVFQQKLAVSSRVAVAQAGKSLGDI
                     AVPEGSVGFATVCSIVINGVLLKHGIPIDSKFGGILQMRGGKILRFVELIHYAGSSLD
                     PSEIFIRGKMTSVRSVARTGEGKILANFREIPALSLPLVERVMEDFAGAGLHGVLSIG
                     HIGHPVCQTHVDMNKIGMILIGGLNPVAAVCEEGLTVDNKAMSTVMEFEKMRDIETI"
     gene            complement(1431..>3755)
                     /locus_tag="PLR47_08470"
     CDS             complement(1431..>3755)
                     /locus_tag="PLR47_08470"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOG13019.1"
                     /translation="MVSSELYGLVEVAHRFLRMNGDAGGGQLFVLDSHRGGGAKGIAA
                     CFYDGTPIRLEERDLQTAEITTRDIDRGLYPHFFLKEISESIRSVRGTMRGKYRIIDS
                     GPARLRISFNLGEDVVPDSIRNGLQSGLIQHIFVIGQGTAAVAGQAVADTLTHCLRGS
                     RLDIRSRIASELSGFGLRDDLSDTLVIPITQSGTTTDTNRAVSMARARGAQILSIVNR
                     RQSDITASSHGVFYTSDGRDIEMSVASTKAFYAQLVAGQLLALYFAKLLGTMSDEAIV
                     RRIRHLENIPQAMQRLFDRRDEIAACVTQGAGKRYWAVVGSGPNKAAADEIRIKLSEM
                     CYKTISSDIVENKKHIDLSAEPLILVCAAGNPDVVLDDIAKDIAIFRAHKAATVVFAD
                     EGDLRFHPAADAVIAVPKTPAPLSVILNTMAGHLWGYYAACAIDGEAQLFRAFRGRLA
                     LGRLQKKKTKLSVLDVFDLMADPSFRRIINDFCSRFQSRLQGGALSALGGKTIADLTL
                     LLKYASGKLPLQDIRQDFPGHEDLVQPFDLLDETLGAAIDELTRPIDAIRHQAKTVTV
                     GTSRKEKEPQGVVFDLLGALNFSVKNLTWRNIRTIHRLQPSVGAVEGYTLYRVRGLDE
                     KGNPGPASTIAVCAKGGAALRMSSRAEQPTTLMGVKKTIVSTGHAYLGRGKIDSASIM
                     VIPLQETDGRIGNLLLIHIAFNERLSGPEKIEVLGHRYDDIRNLINEYNLAWNDRYME
                     ALPLECLFGESVEEIARRIQARCNPSPVKNTAFT"
BASE COUNT          731 a         1072 c         1142 g          810 t
ORIGIN      
        1 cccttgaacg ggccgatggc gttgttgaac tggacgcgga agccgcggtt gacccgcacc
       61 ttcccctgat cgtccatcca gggcacacga aagatcacgg ccctctccgg ctcgacaatc
      121 cgttcgtaga tgctggcctt tacgaattca gggtgctttt ttacggtagg ctccagcgtt
      181 tccatgactt ccgcgaccgc ctgatgaaat tcgggctggt ccggatcggt ctgtttgact
      241 ttctcgatga cttcttgaat gactgacata cggttaatcc ccctgtaaat atgatttttt
      301 ggttaaattg tctcaatatc gcgcattttc tcaaattcca tgacggtgga catggctttg
      361 ttgtccaccg tcagtccttc ctcgcaaacc gccgccaccg gattcaggcc gccgatcaga
      421 atcatcccga ttttattcat atccacatgg gtctgacaaa ccgggtgtcc aatgtggccg
      481 atggaaagaa cgccgtgcag gccggccccg gcgaaatcct ccatcacccg ctccaccaga
      541 gggaggctga gcgcggggat ttcccggaag ttggccagaa tttttccttc gccggtcctg
      601 gcgacgcttc ggacggacgt cattttgccg cggatgaaga tttccgaagg gtccagcgag
      661 gagccggcgt agtgaatcag ctccacgaaa cgaaggattt tcccgccgcg catctggaga
      721 atgccgccga atttggagtc gatcggaatg ccgtgcttca acagaacgcc gttgatgacg
      781 atgctgcaga cggtggcgaa gccgacgctg ccttccggca ccgcgatgtc ccccaggctt
      841 tttccggcct gcgccacggc cacgcgcgag ctgacggcca gtttttgctg aaacacgggc
      901 ttcatgatgg agagggcccg ggaaaaatgc tctttcggaa agaaggagac gttgaccggc
      961 accaggccgc gcttcttttt gacgtcaaag gtggttcgaa acgagagaag gtcgatgcgc
     1021 gaaatggaaa ggccgatctt atcctgcacg cgggcgtcgg aaatttcggc aaatccctgt
     1081 tcggtgatta cgcggccgtc gcggcggccc acgagtttgg tgagcccccg ttcatccatg
     1141 atcttcaggt ggtagcggac cgttctctcg ctgagctgca cgtgataatc cgacatccgg
     1201 cgggcaatga cgcgcgcgcc catcggatct gggctttcgt ggaggatctt cagaatcaga
     1261 agaaccttcc gttcaatgtc ttctggttta aaaagctctt tcattcgact tccaatcggc
     1321 aatcggcaat cgtttgccgt atccctactt gcaaaccgtt tgtttgtcaa ataaaatttt
     1381 cggccgcttc gaaagagcgc tccggaaggc aaaaagagcg gggaagggcc tcacgtgaag
     1441 gccgtgtttt tcacggggga agggttgcag cgggcctgaa tccgcctggc gatttcctcg
     1501 acggattccc caaacaggca ttccagcggc agcgcctcca tgtaccggtc gttccacgcc
     1561 aggttgtatt cgttgattaa attgcggatg tcgtcatacc ggtgtcccag gacctcgatt
     1621 ttttccggcc cgctcaagcg ctcattgaag gcgatatgga tcagcagcag gtttccgatc
     1681 cggccgtccg tttcctgcag ggggatgacc atgatggaag cggaatcgat ttttccccgg
     1741 cccagataag catggcctgt gctgacgatg gttttcttga cccccatcag agttgtgggc
     1801 tgctccgcgc gggaggacat gcgcagggct gcgccgcctt tggcgcatac cgcaatggtc
     1861 gaagccggtc cgggatttcc cttttcgtcg aggccgcgca cacggtagag cgtgtagcct
     1921 tccaccgccc ccacggacgg ctggaggcgg tgaatcgtcc tgatgttgcg ccaggtcaga
     1981 ttttttacgg agaagttcag cgctccgagc aggtcgaaaa caacgccctg gggctctttt
     2041 tcctttcggc tggttcccac cgtcacggtt ttggcctgat ggcggatggc gtcgatcgga
     2101 cgggtcagtt catcaatggc ggctcccagg gtttcatcca gcaggtcgaa cggctggacc
     2161 aggtcttcgt gtccggggaa atcctggcgg atgtcctgaa gcggcagttt cccggatgcg
     2221 tacttcagca gcagcgtgag atcggcgatg gttttgccgc ccagcgcgct caacgcgccg
     2281 ccctgaagcc gggattgaaa ccggctgcag aaatcgttga tgatccggcg gaaggaagga
     2341 tcggccatca gatcaaaaac gtccaaaacg gataacttcg tttttttctt ttgcagccgg
     2401 ccgagcgcca ggcggccgcg gaacgccctg aagagctgcg cttccccgtc gatggcgcag
     2461 gccgcgtaat agccccacag gtggcccgcc atggtattga gaatcacgga aaggggggcg
     2521 ggtgtctttg gaacggcaat gaccgcgtcg gccgcgggat gaaagcgcaa atccccttca
     2581 tcggcgaaaa cgacggtcgc cgctttgtgc gcccgaaaga tggcgatgtc cttcgcgatg
     2641 tcgtccagta cgacatcggg attgccggcg gcgcagacca gaatcagcgg ctcggcggac
     2701 aggtcgatgt gctttttatt ttccacgatg tccgaagaaa tggttttgta gcacatttcg
     2761 gaaagcttga tgcggatttc gtcggccgcc gccttgttgg ggccgctgcc cacgacggcc
     2821 cagtagcgct tgcccgcgcc ctgcgttaca caggcggcga tttcgtcgcg ccggtcgaac
     2881 aagcgctgca tggcttgcgg aatgttttca agatgccgga tccggcggac aatggcttcg
     2941 tcgctcatcg tccccagcag tttggcgaaa tacagggcca gcaactgtcc ggcaacgagc
     3001 tgggcgtaga aggccttggt ggaggcgacg gacatttcaa tgtcccgtcc gtcgctggtg
     3061 taaaaaacgc cgtgcgacga ggcggtgata tccgactggc ggcggttgac gatggagaga
     3121 atctgcgcgc cgcgcgccct ggccatggac acggcccggt tggtgtccgt ggtggttccg
     3181 gactgcgtga tggggatgac cagcgtatcc gacagatcgt cccgaaggcc gaagccgctc
     3241 agctcagacg cgatgcgaga ccggatgtcg aggcggctgc cccggaggca gtgcgtcagg
     3301 gtatccgcca ccgcctggcc ggccaccgcc gccgttcctt ggccgatgac gaagatgtgt
     3361 tggatcaaac cgctttgcag accgtttcga atcgaatcgg gaaccacatc ctcgcccaga
     3421 ttgaaagaaa tccgcaaacg ggccgggccg gagtcgatga tccggtattt gccccgcatt
     3481 gttccccgga cggagcggat ggattctgaa atttctttga gaaagaaatg aggatagagg
     3541 ccgcggtcaa tgtcccgggt cgtgatttcg gcggtttgca gatcccgctc ttcgaggcga
     3601 atcggggttc cgtcgtagaa gcaggcggcg atcccctttg cgcctccgcc gcggtgtgaa
     3661 tccagaacaa acagttgccc tccgccggcg tcgccgttca ttcggaggaa gcggtgcgcg
     3721 acctccacca gaccgtagag ctccgaggag accat
//
LOCUS       DAOSIM010000202         3749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_21529_length_3749_cov_7.452626, whole genome shotgun sequence.
ACCESSION   DAOSIM010000202 DAOSIM010000000
VERSION     DAOSIM010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21529_length_3749_cov_7.452626"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1528)
                     /locus_tag="PLR47_08475"
     CDS             complement(140..1528)
                     /locus_tag="PLR47_08475"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG13020.1"
                     /translation="MSKNVLLINPWIYDFAAYDFWLKPLGLLYLGGLLRANHHRISCI
                     DCLDPYNSLMLQKSGKASKRHAYGHGRFFRQVIEKPSCLEAIDRSYCRYGMDPSVFRK
                     EIARFSDTDVVLVTSMMTYWYPGVFDVIKILKEMLPGVPVILGGKYASLCHDHAAANS
                     GADFAVRGRGEKQILELMRSVFGEDIVFNPEEDDLDALPYPAFDLLGRIDQVPIITSL
                     GCPYRCSYCSSHLFYDRHMKRNPGKVADEIEYWHRRFGVMDFSFYDDALLADPESLII
                     PLLHDIQRRNLSCRFHCPNGLHVREIDAPLSRLLFESGFKTIRFGFETANFEAQRETG
                     GKVRNEELKRAVAHLKEAGYKTRDIGVYLLCGLPGQQAREVEESIDFVVRCGARPVLA
                     EYSPIPGTPMWPEAVAASIFDIQREPLYHNNTLLCCRNNDFSYEAYRGMKERIQALPK
                     ENGFGNGTSDSN"
     gene            1679..2128
                     /gene="dtd"
                     /locus_tag="PLR47_08480"
     CDS             1679..2128
                     /gene="dtd"
                     /locus_tag="PLR47_08480"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOG13021.1"
                     /translation="MRAVLQRVDRAGVNINSRLFSSISQGLLVLLGVEKGDSRKDADY
                     VLEKTVNLRIFEDEQGKMNRSLADIGGEMMVVSQFTLLADCVKGRRPSFAGAEEPEKA
                     NELYEYFVSEASMKVKKLATGRFQEMMQIELINNGPVTILLDSRKTV"
     gene            2131..2619
                     /locus_tag="PLR47_08485"
     CDS             2131..2619
                     /locus_tag="PLR47_08485"
                     /inference="COORDINATES: protein motif:HMM:NF018624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HOG13022.1"
                     /translation="MNENDIRIDKDGIWYYRGAHMFRKDILCIFFEHLKRDDSGRYFI
                     ELNGEICGLEVEDTAFVVTAVCKIQDACGSETLEILLSDDRKEALDAGTLAVGKDNVL
                     YCRVKNNRFPARFTRKSYYQLAEFIEPDGDENEFCVVFDGRKYPIALASHNHDNGGNH
                     VR"
     gene            2609..>3749
                     /gene="guaB"
                     /locus_tag="PLR47_08490"
     CDS             2609..>3749
                     /gene="guaB"
                     /locus_tag="PLR47_08490"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOG13023.1"
                     /translation="MLDDVVRESLTFDDLLLVPAASSILPRDVETSTWLTQRIKLNIP
                     IVSAAMDTVTESRTAICMAQEGGIGIIHRNMSIERQAIEVDRVKKSESGMVVDPITIE
                     PERKVSDALALMHQYSISGVPVVKNEKLVGILTNRDLRFEENLDQPVSNVMTKKNLIT
                     VKSSITLEESKKLLHKHRIEKLLVVDDENRLKGLITIKDIEKIRRYPNACKDSLGRLR
                     VGAAVGIIDREPRIEALLAAGVDVIAIDTSHGHSAGVIDAIRSTKANFPKCELIAGNI
                     ATVEGAQALIDAGVDAVKVGVGPGSICTTRIIAGVGVAQMSAIRDTYKVASRQGIPVI
                     ADGGIKYSGDIVKALGCGANAVMIGGLFAGTEESPGETILFQGRSY"
BASE COUNT          949 a          981 c         1051 g          768 t
ORIGIN      
        1 gatgcaaaac aaaatgatga aagaggccgc aaacagcgtc atcacgaatt ccagcggatt
       61 ggccagttga aaggagcttt tcaggagaag gacgccgaac accagaaaaa atccggacaa
      121 cagaaaaatg acgatgtttt taattcgaat cagaagttcc attcccaaaa ccgttttctt
      181 tcggcagcgc ttgaatgcgc tctttcatcc cgcgataagc ctcataactg aaatcattgt
      241 tgcggcagca caacagagtg ttgttatgat acagaggctc gcgctgaatg tcaaatatgg
      301 aagcggcgac ggcttccggc cacatggggg ttcccggaat cggcgagtac tcggccagca
      361 cgggccttgc gccgcaccgg acgacaaaat cgatgctctc ttccacctcc cgggcttgct
      421 ggcccggcag gccgcacaaa aggtaaactc cgatgtcccg tgttttgtat cctgcctctt
      481 tcagatgcgc gaccgcgcgc ttcagttcct catttcgcac cttgccgccg gtttcccgct
      541 gcgcctcgaa attggctgtt tcaaagccga agcgaatcgt tttgaagccg gattcaaaga
      601 gcagacggct gagcggcgcg tcgatttccc ggacatgcag gccgttcggg cagtgaaacc
      661 ggcaggatag attgcgtctt tgaatgtcgt gaagcagagg aatgatcagg ctttcgggat
      721 cggccagaag ggcgtcgtcg tagaagctga aatccatgac gccgaatcgc cgatgccagt
      781 attcgatctc gtctgcgact tttccgggat tgcgcttcat atgccggtcg taaaacaaat
      841 gggaggaaca gtagctgcag cggtagggac agcccagcga agtgatgatg ggaacctggt
      901 caatccggcc caacaggtca aacgcagggt agggcagggc atccagatcg tcttcttcgg
      961 gattgaagac gatgtcttct ccaaaaacgc ttcgcatcaa ctcgagaatc tgcttttcac
     1021 cccgtccgcg aacagcgaaa tccgccccag aattggccgc cgcatgatca tggcaaaggg
     1081 aggcatactt tcctcccagg atgacgggaa cgcccggcag catctccttg agtattttaa
     1141 tcacgtcaaa aaccccggga taccagtagg tcatcatgga cgtgaccagc acgacatccg
     1201 tatcggagaa gcgtgcaatc tcttttcgga aaacggacgg atccattcca tatcggcagt
     1261 agctgcggtc aatcgcctcg aggcaggacg gcttttcaat gacctgccgg aagaagcggc
     1321 catgcccgta agcgtggcgt ttggacgctt tgccgctttt ttgcagcatg agcgaattat
     1381 aaggatcaag gcaatcaatg caggaaatgc gatgatggtt ggcgcgcagc agcccgccca
     1441 ggtataaaag ccccaggggt ttcagccaga aatcatacgc cgcaaaatca taaatccagg
     1501 gattgattaa cagaacgttc ttgctcatgg ggtgtcttta gcatacaagc ccataattta
     1561 caaagtgcat gaagcatatg tcgagaggca attcgtgatc agcagcagga ttttcagaaa
     1621 gaacttgaag actcgggcaa ttgttgctaa catgcccaaa ctttttaagg gatgcttcat
     1681 gcgggcagta cttcaaagag ttgaccgggc cggcgtcaac ataaattcac gattattttc
     1741 ctctatttcg caagggttgc tggtattgct tggcgtggaa aaaggcgatt cccggaaaga
     1801 cgcggattat gttctggaaa aaacagtcaa tttgcgaata tttgaggatg aacagggaaa
     1861 gatgaaccgc tcgcttgccg acattggcgg cgaaatgatg gtggtttcgc agtttacact
     1921 gctggccgac tgcgtcaagg ggcgaaggcc gtcgtttgcc ggggcggaag agccggagaa
     1981 ggccaatgag ctgtatgaat attttgtgtc cgaggcgtct atgaaggtga agaagctggc
     2041 caccggcagg tttcaggaaa tgatgcaaat cgaactgatc aacaacggac cggtgacgat
     2101 tctgctcgac agcaggaaaa cggtttgacc atgaacgaaa acgacattcg gatcgacaaa
     2161 gacgggatct ggtattaccg cggcgcgcac atgttccgca aagacatttt gtgcattttc
     2221 ttcgagcacc tgaagcggga cgacagcggc cggtatttca tcgagctcaa cggagaaatc
     2281 tgcggactgg aggttgaaga cacggccttt gtcgtaacgg cggtttgcaa aatccaggac
     2341 gcctgcggtt ctgaaacact ggaaatcctg ttgagcgacg accggaagga ggcgctggac
     2401 gccggcacgc tggccgtcgg caaagacaac gtcctgtatt gccgcgtgaa aaacaaccgg
     2461 ttccccgcgc gctttacccg gaaaagctac tatcaactgg cggaattcat tgagcccgac
     2521 ggcgatgaaa acgaattttg cgtcgttttc gacggtcgga aatatccgat tgcgcttgct
     2581 tcccataacc atgataacgg aggaaaccat gttagatgat gttgtaagag agtccctgac
     2641 gtttgacgac ctgctcttgg tgcccgcggc ttccagcatt ttgccgcgcg acgtcgaaac
     2701 atccacgtgg ctgacgcaga gaatcaaact gaacattccc atcgtctcgg cggccatgga
     2761 cacggtgacg gaatcccgca cggccatctg catggctcag gagggcggca tcggcattat
     2821 tcaccggaat atgagcatcg aacgccaggc catcgaagtg gaccgggtga aaaaatcgga
     2881 aagcggcatg gtggtcgatc cgatcaccat cgaaccggaa agaaaagtca gcgacgcgct
     2941 ggccctgatg caccagtatt ccatctccgg cgtgccggtc gtcaaaaatg aaaaactggt
     3001 cggcattctg accaaccgcg atctgcgctt tgaagaaaac ctggatcagc ccgtgtccaa
     3061 cgtgatgacc aaaaaaaatc tcatcaccgt aaagtccagc atcaccctgg aagaatccaa
     3121 aaagctgctc cacaaacacc gcatcgaaaa gcttttggtc gtggacgatg aaaaccggct
     3181 caaaggcctg atcaccatca aggacattga aaaaatccgc cgctatccca acgcctgcaa
     3241 ggactcgctc ggaaggctgc gcgtcggcgc ggccgtgggc atcatcgacc gggagcccag
     3301 aatcgaggcg cttctggcgg cgggtgtgga cgtgattgcc attgacacct ctcacggaca
     3361 ttcggcgggc gtgattgacg ccatccgctc caccaaggcg aatttcccca aatgcgagct
     3421 catcgcggga aacattgcaa cggtcgaggg ggcccaggcg ctcatcgatg ccggagtgga
     3481 tgcggtaaag gtcggagtgg ggcccggctc catctgcacc acccgcatta tcgcgggcgt
     3541 gggcgtggcg cagatgtccg cgatccgaga cacctataaa gtcgcctccc gtcagggcat
     3601 tcccgtcatt gccgacggcg gcatcaagta ttccggcgac atcgtcaagg ccctgggctg
     3661 cggcgcaaac gccgtcatga tcggcggact gtttgccgga accgaagaaa gcccaggtga
     3721 aacgattttg ttccaggggc gcagttaca
//
LOCUS       DAOSIM010000203         3748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_21538_length_3748_cov_10.959112, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000203 DAOSIM010000000
VERSION     DAOSIM010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21538_length_3748_cov_10.959112"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PLR47_08495"
     CDS             complement(<1..351)
                     /locus_tag="PLR47_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG13024.1"
                     /translation="MLRILYQPYKWLIFAPYLAVSTLFFGSLTVVLAVVTNPRITSFI
                     CGTVWSRLNGYLTPIRVRVTGRGNIDPTQSYVIVANHQSQYDIFVLYGWLGIDFKWVM
                     KQELRKVPGIGIGCE"
     gene            complement(446..928)
                     /locus_tag="PLR47_08500"
     CDS             complement(446..928)
                     /locus_tag="PLR47_08500"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOG13025.1"
                     /translation="MQVFAASDIVEVAIRIEENGINFYKFAEQIAKQEDAKKLFSQLA
                     QAEAAHKRTFEKFFAAMEKYNPPEQYAGEYSTYLRNYVDNNLIFTKEIMDRQLAKVTD
                     TVGAFEFAMQRELDSVLYYHEIKNLVPAAQHEAIDKIIEEERRHYTMLCDMKKRYENR
                     "
     gene            complement(1010..2071)
                     /gene="corA"
                     /locus_tag="PLR47_08505"
     CDS             complement(1010..2071)
                     /gene="corA"
                     /locus_tag="PLR47_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOG13026.1"
                     /translation="MTLAMKRRSKKAGLSPGTLMHIGHAYEDSSRIRLMRYNEAALSE
                     KEIFSPEELIEEKKKSGILWVQVDGLQDVGRLEEIGRIFGLHPLILEDILNTDQRPKA
                     EDFTDYIFIVLKNIQPDAAPNLHAEQISILLGPNFVLSFQEKAGTLFDPIRERIRTDK
                     GRIRKAGADYLAHHLMDAIVDQYFVVLEDIDDKIELLEDDLIRKTTPDKLQAIHLLKR
                     ELILLRKSLWPLREAIGLLQRSDSPLIADSDLIYFKDISDHIIAVVDTVDTFRDMLAG
                     MLDIYLSSASMRMNQVMKVLTIIATIFMPLTFLAGIYGMNFANMPELGWRWGYFAVLG
                     VMLTIALGMLMLFRRKKWI"
     assembly_gap    2217..2226
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2227..3457)
                     /locus_tag="PLR47_08510"
     CDS             complement(<2227..3457)
                     /locus_tag="PLR47_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOG13027.1"
                     /translation="MDMVNAAASVIKNIRIQDILDMAIIAGMIFALLMWFKTRASRFV
                     LIGILLLGGVYLAARFFQLYLTVIVLQGFFAILLFVLVVIFQEDLRGFFERIAMLGQL
                     RKEAPPLSRLQKTANIIAETAGNLAKKRVGALLVIQGNDPLDRHLNGGIPLHGTASEP
                     LLESLFDPHSPGHDGAVILKDDRVAMFGCHLPLSVNTARHGNIGLRHTAALGLTERSD
                     ALCVVVSEERGTISVSEGESLSVVPGASTLHEVLEKYYAKYRPAPKKRPLRAWLSENT
                     KEKLIAVGLAFVLWATFGYQRDTVRRDFFVPLEYKNMPAGWQFVAPPVKEAKITLQGP
                     EQAFRLMNEDSLKLSLDVSALAQKNQEFSLNRNMVNTPNGLSIAEISPVKIKVFASRQ
                     IVAHLPVHVATVNRLPDH"
BASE COUNT          829 a          986 c         1054 g          869 t
ORIGIN      
        1 ttcgcagccg atcccgatgc cgggcacttt tctcaattcc tgcttcatca cccatttgaa
       61 gtcaatcccc agccagccgt acaggacaaa gatatcatac tggctttgat ggttcgcgac
      121 gatgacatac gactgcgttg ggtcgatgtt gcctcttcca gtgacccgaa cccggatcgg
      181 cgtgagatag ccgttgagac gagaccagac ggttccgcag atgaagctgg taatccgggg
      241 atttgtgaca acggccagca ccaccgtcag agacccgaaa aagagggtgg agaccgccag
      301 atacggagca aagatcaacc acttgtacgg ttgatacagg attctcagca ttttattcac
      361 cgttttgttt ccaaataaaa attcggatca tcgtttgatc gaacggctga ataaggcttg
      421 ggccggaccc gtacgggcgt tttcgctatc ggttttcgta gcgttttttc atgtcgcaca
      481 gcatggtgta gtgccgtctt tcctcttcaa tgattttgtc gatggcttca tgctgggccg
      541 ccggaaccag gtttttgatt tcatggtagt acaggaccga atccagttcg cgctgcatgg
      601 caaactcaaa ggcccccacc gtgtccgtca ctttcgcgag ctgccggtcc atgatttctt
      661 tggtaaagat caggttgttg tccacgtagt tgcgcagata ggtgctgtat tctccggcat
      721 actgctccgg tggattgtat ttttccatgg cggcgaaaaa cttttcaaac gtgcgcttgt
      781 gcgcggcttc cgcctgcgcg agctgggaga agagcttttt ggcgtcttcc tgcttggcga
      841 tctgttcggc gaatttgtaa aaattgattc cattttcctc aattctaatc gccacttcga
      901 cgatgtcgct tgctgcgaat acctgcatag ggtttcctcc tgaaagaata tgatgaccgc
      961 ttaaaactga acatatttta acggctattg caaccttttt ggcgatgttt caaatccatt
     1021 ttttcctgcg gaacaacata agcattccaa gggcgatcgt caacatgacg cccagaacgg
     1081 caaagtagcc ccaccgccag cccagctccg gcatattggc gaaattcatg ccgtagatgc
     1141 ccgccagaaa cgtcaacggc atgaaaatag tggcgataat ggtgagaacc ttcatgacct
     1201 gattcatccg catgctggcg gaggacaggt agatgtccag catgccggcc agcatgtccc
     1261 ggaaggtgtc caccgtgtca acgacggcga taatgtggtc ggaaatgtct ttgaaataaa
     1321 tcaggtccga atcggcaatg agcggggaat ccgaccgctg caagagaccg atggcctccc
     1381 gaagcggcca gagcgacttg cgcagcaaga tcagttctct tttcagcagg tggatcgcct
     1441 gcagtttgtc gggcgtggtt ttccggatga ggtcatcttc caggagttca attttgtcgt
     1501 cgatgtcctc cagcacaaca aaatactgat cgacgatggc gtccatcaga tgatgcgcca
     1561 ggtaatccgc ccctgccttg cggattcgtc ccttgtcggt gcggattctc tcgcggatgg
     1621 gatcgaacag cgttcccgct ttttcctgaa aggacaaaac gaaattcggg cctagaagga
     1681 tgctgatctg ctccgcgtgc aggttcgggg cggcgtcagg ctggatgttt tttaaaacga
     1741 tgaagatata atccgtgaag tcttccgctt tcggccgctg atcggtgttg agaatgtctt
     1801 ccagaatcag gggatgaagg ccgaagatgc ggccgatttc ctccagacgc ccgacgtcct
     1861 gcagaccgtc cacttggacc cagaggatgc ccgatttttt cttttcctcg atgagttcct
     1921 ccggcgaaaa tatttccttt tccgaaaggg cggcttcgtt gtagcgcatc agccgaatgc
     1981 gggatgaatc ctcgtaggca tggccgatgt gcatcagggt ccccgggctc aatccggcct
     2041 ttttcgatct tcttttcatg gcgagggtca tgggtttggc tccttgggct tgacgcggac
     2101 ggtgacccgc acctcggcgg attttccgtc cgggaaagag gcgccgggga tggtgttgac
     2161 cttgagcgcc accgtcatgg aggaagtgac gctttgcaga tcgaggggct ccgtttnnnn
     2221 nnnnnnggtg atccggcagc cggttgacgg tcgcgacgtg gaccggcaga tgcgcgacaa
     2281 tctgccgcga cgcgaaaacc ttgattttta cggggctgat ttcggcaatc gacaaaccgt
     2341 tgggcgtgtt caccatgttc cggttcaggg aaaactcctg atttttctgc gccagcgccg
     2401 atacatccag cgagagcttc aacgaatctt cattcatcag ccgaaaggcc tgttcgggtc
     2461 cctgcagggt tattttggcc tccttcaccg gcggggcgac gaactgccat ccggccggca
     2521 tgtttttata ctcgagggga acgaaaaaat cacggcgcac cgtgtcccgc tgataaccga
     2581 aggttgccca caacacaaag gccagcccca cggcaatcaa tttttccttt gtgttttcgc
     2641 tgagccaggc ccggagaggt ctttttttcg gtgcgggccg gtatttcgcg tagtattttt
     2701 ccaaaacctc gtgaagcgtc gaggcgccgg ggacgacgga aagcgactcc ccctcggaga
     2761 cggaaatggt gccgcgctct tcggacacga caacgcacag ggcgtccgaa cgctccgtca
     2821 gccccagcgc ggccgtgtgg cgcagaccga tgtttccgtg tcgcgcggta ttgacggaca
     2881 ggggcaaatg acagccgaac atcgccacgc ggtcatcctt gaggatcacg gccccgtcat
     2941 ggcccgggga atggggatcg aaaaggcttt ccagaagcgg ttcgctcgcg gtgccgtgta
     3001 agggaatgcc gccgttcaaa tgtcggtcca gcgggtcatt cccctggatg accagcaagg
     3061 cgccgacgcg cttcttggca agattacccg cggtttccgc gatgatgttc gcggtttttt
     3121 gcagccgcga caggggcgga gcctctttgc gaagctggcc cagcatggca atgcgctcaa
     3181 agaagccgcg caggtcttcc tgaaagatca ccaccaggac gaacagcaaa atggcgaaga
     3241 atccctggag cacaatcacg gtaagataga gctgaaaaaa ccgggcggcc agatacacgc
     3301 cgcccagaag caggatgccg atcaggacaa atcgcgacgc cctggtttta aaccacatca
     3361 ggagggcgaa aatcattccg gcgataatcg ccatgtccag gatgtcctga attcgtatgt
     3421 ttttgatgac ggacgcggcg gcgttgacca tatccataac ggcacgtttc cagtttgaga
     3481 gaaatagacg gcaatgtata acggcatttg atgacggatg tcaaggaaca aggcaaaaaa
     3541 acgccccccc ggagaaaggg gaggcggatg gtaggggcaa accgcgagaa actccttttc
     3601 ccgaagcgtc gccggcaaat gccgtcttcg ggaaaggggc tcttgctcta gtagtatttt
     3661 ccggcgatat agtcgctcaa ctgattgatg agaaaatcct tgttggagat gatcagcttc
     3721 aggacgtcgc cgatggaaat gatgccga
//
LOCUS       DAOSIM010000204         3744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_21570_length_3744_cov_7.718352, whole genome shotgun sequence.
ACCESSION   DAOSIM010000204 DAOSIM010000000
VERSION     DAOSIM010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21570_length_3744_cov_7.718352"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="PLR47_08515"
     CDS             complement(<1..1000)
                     /locus_tag="PLR47_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOG13028.1"
                     /translation="MNSGDTAWVLTAGALVLLMTIPGLAFFYGGLVRRKNVLSILMQC
                     FIIVCVISLQWVLFGYSLAFGPDFHGIIGTLEWAGLAGVGAAPNSNYAATIPHSVFMI
                     FQAMFAIITPALIIGAYAERVKFPAFLLFTLLWATFVYDPLAHWVWGAGGWLKSLGGL
                     DFAGGIVVHVSSGISALVLALLLGKRVGYNHKPIRPHNLPFTVLGAALLWFGWFGFNA
                     GSALAADGLAANAFVTTNTATAAAGLTWALIEWWHNGTPTILGVATGAVAGLVAVTPA
                     CGFVNPMNAIFIGIIVALVCYTAVAVIKGKLGYDDSLDAFGVHGVGGTVGTIATGLF"
     gene            1332..>2510
                     /locus_tag="PLR47_08520"
     CDS             1332..>2510
                     /locus_tag="PLR47_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOG13029.1"
                     /translation="MIPYARVSSGISGLDKILNYLQMGDNVVWQVDHIEDYKKFVDPY
                     VEQALADRQRLVYMRFANHPALLEARPGIKICQLNANSGFESFSTEVHNIIRDEGRDV
                     FYVFDCLSDLLQAWATDLMIGNFFVITCPYLFELNTVAYFAILRSRHSFKTVARIRET
                     TQVLLDICNREGRLCVHPLKAWKRYTPTMFLPHIMEDQACVPILNSADAASFLSCLTD
                     RNATGPERNLDYWDRIFLKAGSLLENPDAAPEKQDMVEILSRVMIGREQRILSLVREY
                     FDLEDLVEIKKRLIGTGFIGGKSVGMLLARNILRKDPSFDWNAVLEIHDSFYVGSDIF
                     YSYMVQNGWWKLLMAQKTDEGYFDVARELKNKMLHGVFPDEIKEQFQLMLEYFGQAPI
                     I"
     assembly_gap    2511..2520
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2521..>3744
                     /locus_tag="PLR47_08525"
     CDS             <2521..>3744
                     /locus_tag="PLR47_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOG13030.1"
                     /translation="SSLLEDAFGNAFAGKYESYFCVNQGTPEERYRHFEEAVRRIFAS
                     TMNEDALTYRLQRGMAQQDEQMALLVQRVSGSHRGSYFFPGLAGVGLSYNTFVWQKGM
                     DPKAGMLRIVFGLGTRAVNRVENDYPRIVALDAPTVKPYTKPEDIKRFSQHEADVLNL
                     RDNEFQTLPTAELLREDLVANLNWIVEEDTEAADYLRAAGRDASQAWIITFDELLSAT
                     PFAKTMSKMLKTLEQIYRYPVDIEFTVNFNAAGDPRINLLQCRPFQTKGHYRRVELPE
                     NISRSRTLIRQDANFMGGSVYQTISRLICIDPRAYTRLTLSEKHEVARLTGKLNRQIG
                     RREAMPTILLGPGRWGTTTPAMGVPVTFSEINNITAIGEIAYQDGALIPDLSFGTHFF
                     QDMVEMDIFYMAIYPE"
BASE COUNT          883 a         1087 c         1030 g          734 t
ORIGIN      
        1 cgaataatcc cgtggcgatc gttccgaccg tcccgcccac gccgtgcacg ccgaaggcgt
       61 ccagagaatc atcgtaaccc agtttgcctt taatgacggc aacggcggtg tagcagacca
      121 gggcgacgat gatgccgatg aagatggcat tcatcgggtt gacgaacccg caggccgggg
      181 tgaccgcaac cagaccggcg acggcgccgg tggccacgcc cagaatcgtc ggagttccat
      241 tgtgccacca ttcaatcagg gcccatgtca aaccggcggc ggctgtcgcc gtattcgtag
      301 tcacaaaggc gttggccgcc aggccgtcgg ccgccagagc gcttcccgcg ttaaacccga
      361 accagccgaa ccacaaaagc gccgcgccta aaacggtgaa cggcaggtta tgcggacgaa
      421 tgggtttgtg gttgtagccg actctctttc ccagcagaag cgccagcacc agggccgaaa
      481 ttcccgagct cacatgcacg acgatgccgc cggcgaaatc cagcccgccc aaagacttga
      541 gccagccgcc cgcgccccag acccaatgcg ccagcggatc ataaacgaag gtcgcccaca
      601 agagcgtaaa gagcagaaag gccgggaatt tcacgcgctc ggcatacgcg ccgatgatga
      661 gcgccggcgt gatgatggcg aacatcgcct gaaagatcat aaagacgctg tgcggaatgg
      721 tcgccgcgta attgctgttg ggagcggcgc cgacaccggc cagtcccgcc cactccagcg
      781 ttccgatgat gccgtgaaaa tccggaccga aggccagcga atagccgaag agcacccact
      841 gcagggaaat gacgcagacg atgatgaagc actgcattaa aatggaaaga acatttttgc
      901 gccggacaag acctccgtaa aaaaatgcca gccctggaat ggtcattaaa agaaccaggg
      961 cgccggcggt taacacccac gctgtatctc cggaattcat gatggtggac ctcctgtaag
     1021 aattttttca ttttttgact tgagcaagga tggtgccatc aggattgtca acttcttgaa
     1081 tattgaaatc acagataatt tatcgatcaa tggccgtttc aaataagaac aatattgtgt
     1141 gatttatcaa gaatcagaat caaaatgtgt acgtggcgtc ttgccggaat ggctggaaat
     1201 ttgttcaaaa atgttcggca aatgacccgt aaaaaagacg aaggatatgc gcccgaacgc
     1261 cggtggcata aaaattgaag accgtttgaa agagacgttt aagatttgca aagaaaagga
     1321 agcagaatgt tatgatccct tatgcgcgcg tcagttccgg catcagcggt ctggataaaa
     1381 tcctcaacta tctgcagatg ggcgacaatg tcgtctggca ggtggaccat atcgaggact
     1441 ataaaaaatt tgtcgatccc tatgtcgagc aggccctggc cgatcgtcag cgcctcgttt
     1501 acatgcgttt tgccaaccac cccgcccttc tggaggcgcg ccccggaatc aaaatctgtc
     1561 aactcaacgc aaattcagga tttgaatcct tttccacaga ggtccacaac atcatccgcg
     1621 acgaaggccg cgacgtgttt tacgtttttg actgtctgtc cgatctgctg caggcctggg
     1681 ccaccgacct gatgatcggc aacttcttcg tcatcacctg cccttaccta tttgagctca
     1741 acacggtcgc gtatttcgcc atcctgcgca gccgccattc tttcaaaacc gtcgcccgta
     1801 tccgcgaaac gacgcaggtt ctgctggata tctgcaaccg cgaaggaaga ctctgcgtcc
     1861 atccgctcaa ggcctggaag cgctacacgc ccaccatgtt tctgccgcac atcatggaag
     1921 accaggcctg cgttccgatt ttaaacagcg cggacgccgc gagctttctg tcctgcctga
     1981 cggacaggaa tgccaccggc cccgagcgca atctcgatta ctgggaccgc atctttctga
     2041 aagccggcag ccttctggaa aatcccgacg cggccccgga aaagcaggac atggtggaaa
     2101 ttctttcccg cgtgatgatc ggcagggagc agcggatcct gtcgctggtg cgggaatact
     2161 tcgacctgga agacctggtg gaaatcaaga agcgccttat cggcacgggg tttatcggcg
     2221 ggaaatccgt cggcatgctg ctggcgcgca acattctgcg caaggatccg tcctttgact
     2281 ggaacgccgt tctggaaatc cacgattcct tctatgtcgg ctcggacatt ttctattcct
     2341 acatggtgca gaacggctgg tggaagctgc tgatggcgca gaaaaccgac gaaggatatt
     2401 ttgatgtcgc gcgcgagctc aaaaacaaga tgctgcacgg cgtgtttccc gacgaaatca
     2461 aggagcagtt ccagctcatg ctggaatatt tcggccaggc gccgatcatc nnnnnnnnnn
     2521 agttcgcttc tggaagacgc cttcggcaac gcctttgccg gaaaatacga aagctatttc
     2581 tgcgtcaacc agggaacgcc ggaagagcgc taccggcatt tcgaggaggc cgtccgcaga
     2641 atcttcgcct ccacgatgaa cgaggacgcg ctcacctacc gcctgcagcg cggaatggcc
     2701 caacaggatg aacagatggc gcttctggtg cagcgcgttt ccggctccca ccgcggcagc
     2761 tacttctttc ccggcctggc gggcgtcggc ctgtcctaca atacgtttgt ctggcagaag
     2821 ggcatggatc cgaaagccgg catgctgagg atcgttttcg gcctgggtac gcgcgccgtc
     2881 aaccgcgtgg aaaatgacta cccgcgcatc gtcgcgctgg acgcgcccac cgtcaaaccc
     2941 tacacgaagc ccgaggacat caagcggttt tcccagcacg aagcggatgt cctgaatctg
     3001 cgcgacaatg aatttcagac gctgccgacc gccgaactgc tccgcgaaga cctggtggcg
     3061 aacctgaact ggatcgtgga ggaagacacg gaagcggccg actatctgag ggccgcgggg
     3121 cgcgatgcga gtcaggcctg gatcatcacg tttgacgaac tgctctcggc gacgccgttt
     3181 gcgaaaacca tgtccaaaat gttaaaaacg ctggagcaga tttaccgcta cccggtggac
     3241 attgagttta ccgtcaattt caacgcggcg ggcgatccgc ggatcaacct gctgcagtgc
     3301 cgtccctttc agaccaaggg ccactaccgc cgggtggagc tgccggaaaa catcagccgg
     3361 tccagaacac tgatccggca ggacgccaac ttcatgggcg gcagcgttta ccaaactatc
     3421 tcccgcctga tctgcatcga cccccgggcg tatacccggc tcacgctttc ggagaagcac
     3481 gaggttgcca ggctcaccgg caagctcaac cggcagatcg gacgccgcga ggccatgccg
     3541 acgattctgc tgggcccggg gcgatgggga acgaccacgc ccgcgatggg cgttccggtc
     3601 acgttttcgg aaatcaacaa cattaccgcc atcggcgaga tcgcttacca ggacggcgcg
     3661 ctcatccccg atctgtcctt tggaacgcat tttttccagg acatggtgga aatggacatc
     3721 ttttacatgg ccatttatcc cgaa
//
LOCUS       DAOSIM010000205         3741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_21597_length_3741_cov_7.837765, whole genome shotgun sequence.
ACCESSION   DAOSIM010000205 DAOSIM010000000
VERSION     DAOSIM010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21597_length_3741_cov_7.837765"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..1283)
                     /locus_tag="PLR47_08530"
     CDS             complement(174..1283)
                     /locus_tag="PLR47_08530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG13031.1"
                     /translation="MNYFIRICTCIMILISLSGCHGAAPEARDSRFTVIAQDMPYPTD
                     QYIRIGYTLKMREYEKEGLDLQRIDILDGSSKAVLFTIGKKDIPKIYKDPMPVNPYYP
                     PDKLDHYYVSLQLPIPLGRTVPKSVTHRFSFRDQQTNRDLSMEGGTFLPDQAMTPIVI
                     ASPVRGKNWLFVNQSTMGYHFNAMFFALGKRGTGERFAFDNVQFNDDLTEYFHGDPAK
                     NESYFNYGESLYAVADGTVVAMRDGLPENSGNDHSVTFKEPIDLAGNYIIIDIGKGRY
                     AFYAHCRTHSIRVKAGDQVREGDVIALLGNSGNSDAPHLHFQICDAPDFFFSRGLPFV
                     LKKYTKIAQSGQDVLPPPKEHMNAMMEELTVISFD"
     gene            complement(1312..>3741)
                     /locus_tag="PLR47_08535"
     CDS             complement(1312..>3741)
                     /locus_tag="PLR47_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOG13032.1"
                     /translation="EKPSRYIGAEVNAVRKENTGVRFLLAFPDTYEVGMSHLGLQILY
                     SVLNALPDAAAERCFAPWPDRERQLRERRLPLTSLETQSPIADFDVIGFSLQYELSYT
                     NMLTMLDLGEIPLQSADRRNEHPLVVAGGPCAFNPAPLAAFIDAFVIGEGEEAVVEIA
                     GALAEAKKQNSGKDAMLEILANLDGVYVPALHKNKIIKKRKVMDLNRWLHPVAPVVPL
                     MQAVHDRIVLEIARGCTRGCRFCQAGMIWRPYRERNARLITEMADRALAATGHDEISL
                     LALSSGDYSCIEPLLQNLMRKHCAQRVALALPSLRVETLSQTLIEEIKRTRKTSFTLA
                     PEAGTDKMRRIINKGNSAEDLLASVDQVFAAGWKSIKLYFMIGLPHEEESDLEGIVDL
                     ASQALRASRGRGQVTVSLSTFVPKPHTPFQWESQLSIEETCARQNFIRQRTHHRHLSV
                     KWHDARMSLLEGLFSRGDEKIGDALLLAWQKGCRFDGWGELFRFDPWQEALREAGIQT
                     DDYLRERSADEPLPWDNIDCGVSRDFLQSERHKADMETDTPDCRYEACQDCGVCDFKN
                     VQNIFSDRDLPEPAAESRTPAESGPEKVYRLNFAKTGRARFLSHLELAAALTRALRRS
                     SLSLCYSAGFHPHPKISFATATSVGMESRQEYLDVTALQHSRDLSILKDEINKALPDG
                     MIITGLQELSFAARDLARSLQGFAYELILPPDTDEEILSRLSRGIENFLAAESFPISR
                     PSKGKTVTRDIRPFVEALTIRTGEKTVDLTVRHAQTGSVRPVDILRHVLGFSEEETLR
                     IGVVKTKTILA"
BASE COUNT          772 a         1003 c         1123 g          843 t
ORIGIN      
        1 agaaaaagcc ggggacgctg cccgtgaagg aagaagacct gccggcgtta atcaaaaagc
       61 tcacggcgga tatgaaacag gcggccaaaa acatggaatt tgaaaaggcc gccgaactgc
      121 gcgacgaaat caaagagctg aatcaaatcc tgctggagat gggttagccg gcttcaatca
      181 aagctgatga ccgtaagctc ctccatcatg gcgttcatat gctctttggg cggcggcagg
      241 acatcctgtc cgctttgtgc gattttcgtg tattttttca ggacaaacgg cagcccgcgg
      301 gagaaaaaga aatccggcgc gtcgcagatc tgaaaatgca ggtggggcgc gtcggaattg
      361 ccggaattcc ccagcagggc gatcacgtcg ccttctctaa cctgatctcc tgccttgacc
      421 ctgatcgaat gcgtgcggca gtgcgcgtaa aaggcataac ggccttttcc gatgtcgatg
      481 atgatgtagt tgccggccag atcgatgggt tctttgaagg tcacgctgtg gtcattgccg
      541 gaattttccg gcagaccgtc gcgcatggcc accaccgttc cgtccgccac ggcgtacagg
      601 gactccccgt aattgaagta ggattcgttt ttagcgggat ccccgtgaaa atattccgtc
      661 agatcgtcat tgaattgcac attgtcaaaa gcaaagcgct cgccggtccc ccttttcccg
      721 agtgcaaaaa acatggcgtt gaaatgatat cccatcgtgg actgattgac aaacagccag
      781 tttttgccgc ggacgggcga agcaatgacg atgggcgtca tcgcctgatc cggcaggaag
      841 gttccgcctt ccatcgacag gtcccggttt gtctgctgat cccggaaaga aaaccggtgc
      901 gtcacgctct ttggaacggt gcggcccaaa ggaatgggaa gctgcaggct gacgtagtaa
      961 tgatccagtt tgtcgggcgg ataataggga ttgacgggca tcggatcttt gtagattttg
     1021 ggaatatctt ttttcccgat ggtaaagagc accgccttgg agctgccgtc gaggatgtcg
     1081 attcgctgca ggtccaggcc ttctttttca tattcccgca ttttcagcgt atagccgatc
     1141 cggatgtatt gatccgtggg gtagggcatg tcctgggcaa tgacggtaaa tcggctgtct
     1201 cgcgcctcgg gggctgcgcc gtggcaccct gaaagactga tgagaatcat gatacatgtg
     1261 cagatcctta tgaaatagtt catttggttc cctccttgat ttttcggatc ttcatgcaag
     1321 aatcgttttc gtcttgacga ctccaatccg cagtgtttct tcttcggaaa agcccaaaac
     1381 atgcctcagg atatcgacgg ggcggacgga gccggtctgc gcgtgccgta cggttaagtc
     1441 cacggttttt tccccggtgc ggatggtcag ggcttccaca aaaggccgga tgtccctggt
     1501 gacggttttt cccttggatg gcctggaaat gggaaatgat tcagccgcca gaaaattttc
     1561 gatgccgcgg gagaggcggc tgaggatttc ctcatccgtg tccggcggaa gaatcagttc
     1621 ataggcaaag ccctgaagag aacgggccag gtcccgggcg gcaaaggaaa gttcctgtaa
     1681 gcccgtgatg atcatgccgt cgggcaaggc tttgttgatc tcgtctttca aaatcgataa
     1741 atcccgtgaa tgctgcaggg cggtgacgtc caggtattcc tgccggcttt ccatacccac
     1801 tgatgtggcc gtggcgaagg agattttggg gtgcgggtga aaccccgccg agtagcacag
     1861 ggaaagggag ctgcgcctga gcgcgcgggt gagcgccgcg gcgagctcca gatgagaaag
     1921 aaagcgggcc cggccggttt tggcgaagtt caggcggtag actttttcgg ggccggattc
     1981 ggcaggcgtc cggctttccg ctgccggctc cggcagatcg cggtctgaga aaatattttg
     2041 aacgtttttg aaatcgcaga cgccgcaatc ctggcaggcc tcgtagcggc agtccggcgt
     2101 gtcggtttcc atgtcggctt tgtgcctttc gctttgcaga aaatcccggc tcacgccgca
     2161 gtcgatgttg tcccagggca gaggctcgtc cgccgagcgt tcgcgcaggt aatcgtccgt
     2221 ttggattccc gcttcccgca gggcttcctg ccacggatca aatcggaaga gctcacccca
     2281 gccgtcaaaa cggcagcctt tttgccaggc gagcaaaagc gcatctccga ttttttcatc
     2341 gccccgggaa aacaggcctt cgagcaggct catccgcgcg tcgtgccact tgacgctgag
     2401 atggcggtga tgggtgcgct gacggatgaa attctgccgg gcgcaggttt cttcgatcga
     2461 cagttgggat tcccactgaa agggcgtgtg cggcttcggg acgaacgtcg aaaggctgac
     2521 ggtgacctgc ccccggccgc gggatgcgcg cagcgcctgg gaggccagat ccacaattcc
     2581 ttccagatcg ctttcttctt cgtgcggcag gccgatcatg aagtacagct tgatggattt
     2641 ccatcccgcg gcaaagacct gatccacgga tgccagcaga tcctccgcgg agttgccttt
     2701 gttgatgatc cggcgcattt tatccgttcc cgcctcaggc gcaagggtga aactggtttt
     2761 acgggtgcgc ttgatttctt caatcagggt ttggctcagc gtttcgaccc tgagcgatgg
     2821 cagggccagg gccacgcgtt gggcgcaatg tttccgcatc aggttttgga ggagcggttc
     2881 aatgcagctg tagtctccgg aactgagagc caaaagagat atttcatcat ggccggttgc
     2941 cgcgagcgcc cggtcggcca tctccgtgat caaccgggcg tttctctccc ggtaaggtcg
     3001 ccagatcatg cccgcctggc aaaaccggca gccccgcgtg cagccccggg caatttccag
     3061 gacgatgcgg tcgtggacgg cttgcatcag cggcaccacc ggcgcgacag gatgaagcca
     3121 gcggttgaga tccatcacct tccgcttttt gatgattttg tttttgtgca gagccggaac
     3181 ataaacgccg tcaaggttcg caaggatttc aagcatcgcg tcctttcccg aattttgctt
     3241 tttggcttct gccagggcgc cggcgatttc caccaccgct tcttcgcctt ccccgatgac
     3301 aaatgcgtcg atgaaagcgg caagcggcgc ggggttgaat gcgcaggggc cgcccgcgac
     3361 aaccagagga tgttcgttgc gccggtcggc ggactggagc ggtatttcgc caaggtccag
     3421 catcgtgagc atattcgtat aggacagttc gtattgcagc gagaaaccga tcacatcaaa
     3481 atcggcgatg gggctttggg tttccagaga ggtcagggga agacggcgct cccgcaattg
     3541 ccgttcccgg tccggccagg gggcaaaaca cctttcggcg gccgcatcgg gaagcgcgtt
     3601 taagacggaa tataaaattt gcaggcccag atgcgacatg cccacttcat acgtgtcggg
     3661 aaaggccagc agaaagcgta cgccggtgtt ttccttccgg acggcgttga cttccgcgcc
     3721 gatataacga ctgggctttt c
//
LOCUS       DAOSIM010000206         3722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_21734_length_3722_cov_8.416144, whole genome shotgun sequence.
ACCESSION   DAOSIM010000206 DAOSIM010000000
VERSION     DAOSIM010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21734_length_3722_cov_8.416144"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1359)
                     /gene="aspS"
                     /locus_tag="PLR47_08540"
     CDS             complement(<1..1359)
                     /gene="aspS"
                     /locus_tag="PLR47_08540"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOG13033.1"
                     /translation="MQFSERVFCGGLSLKDIGRRVLLAGWVDAFRDHGGLLFIHLRDR
                     SGIVQIVFSPDVAANDVCRKAASLRSEYCVAVSGEVRKRLAGTENPNIETGQIEVFVD
                     KLTVLSESDALPFAVSDKAMVAGASSAGSDHVAEDLRLQYRYLDIRRSAMRDNLTARH
                     RIFRCIREFLDSRGFVEIETPVLTASTPEGARDYLVPSRIHPQNFYALPQSPQLFKQL
                     LMIGGMERYFQLARCFRDEDLRPNRQPEFTQLDIEASFIDEEFLYELIEELTVRMFAV
                     GGIELPRPFARMAYTEAMDTTGSDRPDLRFGLRFVDVTDVFSETRYSIFRQILQRGGC
                     IKGINIQGQSEKLSKNVLQNEYAREIAPSFGAKGMTWMRAEGGKLESNIVQFFSEQEL
                     AELKRRFAVEDGDVLILIADPSYTVVTSALGQLRLHLAGRLDLIPENSFYPVWITEFP
                     LFE"
     gene            complement(1551..2630)
                     /gene="asd"
                     /locus_tag="PLR47_08545"
     CDS             complement(1551..2630)
                     /gene="asd"
                     /locus_tag="PLR47_08545"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF006416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOG13034.1"
                     /translation="MLKIAIVGATGIVGQQAIVSLLGHPWFQITKLVASERSATKKYI
                     DALRDANGSIRWWCPEELPADIANMVVEEAASFDPTSVDVIFSTMDAGPAKELEPKYA
                     KTTPVISTASAFRYEPDTPILVGGVNMDHAALLAVQQKNRGWKGFISPKSNCTIAGLV
                     VSLKPILDNFGVKQVVATSLQAMSGAGRTPGLSAMDMMENVVPYIAGEEPKVETEPQK
                     ILGKLAGDKIVNAPFGITATCTRVPVIDGHLVVAFVQTEKPCTPEDVKKVMTEYGKDF
                     AELHLPSSPAHLIDVTDDPFRPQPRVDRDKGGGMTVTVGRIRKDPILENGIKYVCLAH
                     NTKLGAAKGALQTAEYLVKNFLKLV"
     gene            complement(2624..3592)
                     /locus_tag="PLR47_08550"
     CDS             complement(2624..3592)
                     /locus_tag="PLR47_08550"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOG13035.1"
                     /translation="MKKNMIVFDGIQSITQDYRDVFVTIGNFDGVHLSHQHICRKLAA
                     EAGKAGRKSLVITFDPHPKMILHPNIRPFYLITTRAEKLELIEKCGVDATVVIAFDQH
                     YSKTAAEAFVQEFLWNQLAIRKIIIGHDYTFGQARQGNDAFLKTKGRKLGFSVEVIPA
                     FKINDEIVSSTLIRNYILEGDVRTAAKLMGRHYNAAGIVGSGAGRGAGLGYPTANIEP
                     EKELLPPPGIYAAFVLVDGESCMGALNIGAKPTFEDYTSTFEVFLLDYAGDLRGKKIN
                     VLFVEKLRDIVKFDGPESLKRQIAADVEKTRIILKQEKTFGGKLLC"
BASE COUNT          752 a         1061 c         1078 g          831 t
ORIGIN      
        1 ttcgaagagc ggaaactccg tgatccagac cgggtaaaag ctgttttcgg gaatgagatc
       61 cagacggccc gccaggtgca ggcgaagctg gcccagcgcg gaggtcacca ccgtgtagga
      121 aggatcggcg atcagaatga gcacgtcccc gtcttcaacc gcgaagcggc gcttcaattc
      181 ggcgagttcc tgctcgctga aaaactgaac gatgttggat tcaagctttc cgccctcggc
      241 gcgcatccag gtcatgccct tggcgccgaa cgacggcgcg atttccctgg cgtattcgtt
      301 ttgcaaaacg tttttgctga gtttttccga ctggccttga atattgatgc ccttgatgca
      361 gccgccgcgc tgaaggatct ggcggaagat cgaataacgg gtttccgaaa agacgtccgt
      421 cacatcaacg aagcgaagcc caaagcgcag gtcgggccgg tcggagccgg ttgtgtccat
      481 cgcttccgtg taggccatgc gcgcgaaggg cctcggcagt tcgatgccgc cgaccgcgaa
      541 cattcggacg gtcagctctt cgatcagttc gtagaggaac tcttcatcga tgaacgacgc
      601 ttcgatgtcg agctgcgtga attccggctg tcggttgggg cgcaggtctt cgtcgcggaa
      661 gcagcgcgcg agctggaagt agcgctccat gccgccgatc atcagaagct gtttgaagag
      721 ctgcggcgac tggggcaggg cgtagaaatt ctgcgggtga atccggctgg gcaccaggta
      781 atctcgcgcg ccttcgggcg tgctggccgt gagcaccggc gtttcgattt ccacgaaccc
      841 ccgggaatcc agaaattcgc ggatgcaccg aaaaatgcgg tggcgcgccg tcagattgtc
      901 gcgcatggcg ctccggcgga tgtccaggta ccggtactga aggcgcagat cctcggccac
      961 atggtcggag ccggcggacg aagcgcccgc caccatggcc ttgtcggaaa cagcaaaggg
     1021 cagggcgtcg gattcggaca gcaccgtaag cttgtccacg aacacttcga tttgtcccgt
     1081 ttcgatgttg gggttttccg ttcccgcaag gcgttttcga acttcgcccg aaaccgcgac
     1141 gcagtattcg gaacgcaacg aggccgcctt ccggcagaca tcatttgcgg cgacatccgg
     1201 gctgaagaca atctgcacaa tgccgctgcg gtcgcgcagg tggataaata aaagcccgcc
     1261 gtgatcgcgg aaagcgtcca cccagccggc cagaagcaca cggcggccga tgtctttgag
     1321 agaaagcccg ccgcaaaaaa cgcgctcaga aaattgcata tccatcatcc tttccgttta
     1381 taccgtggag gtacaaattt gtgccgcgga gcgtatcaaa actgttctga tgtttcaaga
     1441 agatatcggg atgggccaaa gaccgaaaac ctgctcttcc gccggtgtcc gcgagcgggc
     1501 aaaaaaagcc gcggcatgga agatttccgc gccgcggccc tgttggaaga ttagactaac
     1561 ttcaggaaat ttttcaccag atactccgcg gtttgcagag cgcccttggc tgcccccagc
     1621 ttggtgttgt gcgccaggca gacgtacttg atcccgtttt ccagaatcgg gtccttgcgg
     1681 atgcgaccga cggtgacggt catgccgccg cccttgtcgc ggtccactct gggctgcgga
     1741 cggaacggat cgtcggtgac gtcgatcaga tgcgccggag agctgggcag atgcagttcg
     1801 gcgaagtcct ttccgtattc ggtcatcact ttcttgacgt cttccggggt gcagggcttt
     1861 tcggtctgca cgaacgcgac caccagatgg ccgtcgatga ccggcacgcg ggtgcaggtg
     1921 gcggtgatcc cgaacggcgc gttgacgatt ttatccccgg ccagtttgcc cagaattttt
     1981 tgcggctcgg tttcgacctt cggctcttcg cccgcgatgt agggcacgac gttttccatc
     2041 atgtccatgg cggaaaggcc gggggtgcgc cccgcgcccg acatggcctg cagagaggtg
     2101 gcgaccacct gcttcacgcc gaagttgtcc agaatgggtt taagcgacac gaccagcccg
     2161 gcgatggtgc agttggactt gggagagatg aatcccttcc agccgcggtt cttctgctgc
     2221 acggccaaaa gcgccgcgtg gtccatgttg acgccgccca ctaaaatggg agtatccggt
     2281 tcataccgga aggccgaggc ggtgctgatc accggggtgg ttttggcgta cttgggctcc
     2341 agctccttgg cggggcctgc gtccatggtc gagaaaatga catcgacgga agtcggatcg
     2401 aagctcgccg cttcttccac caccatattg gcgatgtccg ccggcagctc ttccgggcac
     2461 caccagcgga tggagccatt ggcgtcgcgc aatgcgtcga tatatttctt tgtagccgag
     2521 cgctccgagg cgacaagctt ggtgatttga aaccacgggt gtcccagaag actcacgatc
     2581 gcctgctgac ccacgatgcc ggttgcgccc acaatggcaa ttttcaacat aaaagttttc
     2641 ctccaaatgt tttttcctgt ttcaatatta tcctggtttt ctccacgtcc gccgcgatct
     2701 gtcgcttcaa gctttcggga ccgtcgaact tgacaatgtc ccgcagcttc tccacaaaca
     2761 gcacgtttat tttttttccg cgcaggtccc ccgcatagtc cagcaggaag acttcaaacg
     2821 tggacgtgta atcttcaaaa gtcggtttgg cgccgatgtt gagcgctccc atgcagcttt
     2881 cgccgtccac cagcacaaag gccgcgtaaa tgccgggcgg cggcagcagt tccttttccg
     2941 gttcgatatt ggcggtggga taccccaggc ccgcgccgcg ccccgctccg ctgcccacaa
     3001 tgcccgccgc gttgtaatgg cgtcccatca gcttcgccgc cgtccggaca tcgccttcca
     3061 gaatataatt gcggatcagc gtgctgctga cgatttcgtc gttgatctta aaggccggaa
     3121 tcacctccac ggaaaaaccg agttttcgcc ccttggtctt cagaaaagcg tcgtttccct
     3181 gccgggcctg gccgaatgtg tagtcatgcc cgatgataat cttgcggatg gccagttgat
     3241 tccacaaaaa ttcctgcaca aatgcttcgg cggcggtttt ggagtaatgc tgatcaaaag
     3301 cgatgaccac cgtcgcgtca acgccgcatt tttcaatcaa ttcaagcttt tccgccctcg
     3361 ttgtaatcag gtaaaagggc cggatatttg gatgaagaat cattttaggg tgcggatcaa
     3421 aggtgatgac cagcgatttc cgcccggctt ttcctgcttc cgcggcaagc ttccggcaga
     3481 tgtgctggtg gctcagatgc actccgtcaa aattgccgat ggtgacgaac acgtcccggt
     3541 aatcctgcgt tatggattga attccgtcaa aaacgatcat gttcttcttc actgcccttt
     3601 cgtaagttgt gcaacatagc attttgggca ttaaattcaa gtcattttag cttgacaaga
     3661 ctttcggttc ggtatagtca ctttcgcgtg ccgaagtggc ggaattggta gacgcgctag
     3721 gt
//
LOCUS       DAOSIM010000207         3711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_21826_length_3711_cov_6.463621, whole genome shotgun sequence.
ACCESSION   DAOSIM010000207 DAOSIM010000000
VERSION     DAOSIM010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21826_length_3711_cov_6.463621"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PLR47_08555"
     CDS             <1..403
                     /locus_tag="PLR47_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOG13036.1"
                     /translation="MMPTASAVFSDILDSARNVLKGISGRVPLRSINETGMENIQLVP
                     MDDIETKYYFRFTALDRPGVLSKISGILGANDISLATVIQKAREEAGAVPIVMTTYKA
                     KEKNVRRALTKIDRLDIVRDKTILIRIEDEL"
     gene            471..1697
                     /locus_tag="PLR47_08560"
     CDS             471..1697
                     /locus_tag="PLR47_08560"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOG13037.1"
                     /translation="MKYVVLLGDGMADYPSGQLGGKTPLQCAVTPFLDQIASEGTLGL
                     VDTIPEGFTPGSDVANLSVLGYDPKETYTGRGPLEAASQGIALGQGDIAYRCNLVHIG
                     ETQEGPAFMDDFTAGHISSPEARQIVLDLNKTLHSPRCQFYPGVGYRHLLVWRGAAGS
                     PQTTPPHDIPGKPIAPHLPQGDHAEEILGLMHSAAQILNEHPVNAARAAEGKKTANSI
                     WLWGQGGKPRMAPLTEKYKLTGGMISAVDLLNGLGIYAGLKPLRVEGATGYIDTNYEG
                     KAAMALDALKFMDFVFLHLEAPDEMGHEGNAKGKIMAIECFDEKIVGPILTRIGELGE
                     YRILVLSDHPTPIDVKTHVGDPSPFAVLSSKREENLARGLKFTEAQARDAGLIVSPGH
                     RLMDGFLQDWRAFLGK"
     gene            1687..2106
                     /locus_tag="PLR47_08565"
     CDS             1687..2106
                     /locus_tag="PLR47_08565"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOG13038.1"
                     /translation="MESNLTTFRVIYADTDAMGIVYHTNYIRWFELGRNELMRQMGIV
                     YSDLENLGLHLPLTTVFCRYLQPARYDDLLTVETCFDYIKRASIRYSSKIWDENQRKL
                     LVEGYTIHACTSNEGKIRRMPALITGLIEKYSIRKGE"
     gene            2108..2788
                     /locus_tag="PLR47_08570"
     CDS             2108..2788
                     /locus_tag="PLR47_08570"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG13039.1"
                     /translation="MAAKLSKKELKSPDAFQTTFDRVGEYVEENKSRVIIAAAALSCA
                     VLLVLGIYFYWSYYSGSALKLYAKAQDNILKGGDNPETEKANIPILQKLADQYPHSWS
                     GRMAHYHLANIYYNQGDLDRAIASYETFVAKTGSDKTGVRFLALTSLGYAYEARKDFR
                     AALKYFEQAQKGYHTGFEMMSLRNVARAHEALNDKAKAMEYYQKALEKTTDSAAQLYL
                     RRKIAALG"
     gene            2841..>3711
                     /locus_tag="PLR47_08575"
     CDS             2841..>3711
                     /locus_tag="PLR47_08575"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOG13040.1"
                     /translation="MKKELMSGNEAIARGVYEAGVRFAAAYPGTPSTEIMEQFARYDG
                     VYAEWAPNEKVAVEVAIGAALGGAKALAVMKHVGVNVAADPIFTVSYTGIEGALVIIS
                     ADDPSMHSSQNEQDNRNYAKFAKIPMLEPSDPQEAKEYVKLAFAISEQFDTPVFLRTT
                     TRVSHSKSVVTLSEPRVPEDKTALAHHAAKYVMVPINARIRRVEVEKRQKALQKFAET
                     FPENKMEINDPAVGIITAGMPYNYAKDVFPDYSYLKLGMVYPLPVKMIRHFASRVKKI
                     YVIEELDPYLEEQI"
BASE COUNT          895 a         1047 c         1077 g          692 t
ORIGIN      
        1 catgatgccg acggccagcg cggttttcag cgatattctg gacagcgccc gcaacgtgct
       61 gaaggggatt tccggccggg tgccgcttcg gtccatcaat gaaaccggca tggagaatat
      121 ccaactggtt ccgatggacg acatcgaaac gaagtattac ttccgtttca ccgccctgga
      181 ccgcccgggc gttctgtcga agatatccgg cattcttggc gcaaatgaca tcagtctcgc
      241 caccgtgatc cagaaggcga gggaagaagc gggggccgtg cccatcgtga tgacaaccta
      301 taaagcgaaa gagaagaatg tgcgcagggc gctcacgaaa atcgaccggc tcgacattgt
      361 ccgggacaaa accattctca ttcgcatcga agacgagttg tagcgcaaac ccgcaaagcg
      421 ggtcgtttcg aaacccgcgc ggcatgtaaa aatggaaggg gcgtggctcc gtgaaatacg
      481 tggttttact gggagacggg atggcggatt atccgagcgg gcagcttggc gggaaaactc
      541 cgcttcagtg cgccgtaacg ccctttttgg accagatcgc atccgaaggc actctggggc
      601 tggtggacac gattcccgaa gggttcacgc ccggctcgga cgtggccaat ctcagcgttc
      661 tgggctacga tccgaaagaa acctacacgg gccgcgggcc gctggaagcc gcgagccagg
      721 gcattgcgct ggggcaaggc gacatcgcct accgctgcaa cctggtccat atcggggaga
      781 cccaggaggg tccggcgttc atggacgatt ttaccgccgg acacatctct tcccccgaag
      841 ccagacagat cgttctggat ctcaacaaaa cactccattc gccccggtgc cagttttatc
      901 ccggcgtggg atatcggcac ctgctggttt ggcgcggcgc agccgggtct ccccaaacca
      961 ctccgccgca cgacatcccc ggcaaaccca ttgccccgca tctgccgcag ggcgatcacg
     1021 cggaggaaat tctcgggctg atgcacagcg cggctcaaat cctcaacgaa catccggtca
     1081 acgccgcccg cgccgcggaa ggaaagaaaa cggccaactc catctggctg tgggggcagg
     1141 gggggaaacc gcgaatggcg cccctgaccg aaaaatataa gcttacgggc ggcatgattt
     1201 ccgcggtgga tctgctcaac gggctgggca tttacgcggg cttgaagcct ctgcgcgtgg
     1261 agggggccac aggctacatc gacaccaact acgaaggaaa ggccgcaatg gcgctcgacg
     1321 cgctgaagtt catggatttt gtatttcttc atctggaagc gcccgacgag atgggccatg
     1381 aaggcaacgc gaaaggcaag atcatggcca tcgagtgttt cgacgaaaaa atcgtcggac
     1441 cgattctgac gcgcatcggc gaactggggg aataccgtat tctggtgctc agcgatcatc
     1501 cgacgccgat cgacgtgaaa acccacgtgg gcgatccgag cccctttgcg gtcctgtcat
     1561 ccaaacggga agaaaacctt gcccggggac tgaaattcac cgaagcgcag gcccgcgacg
     1621 ccggtctgat cgtgtctccc gggcatcggc tgatggacgg cttccttcag gactggagag
     1681 cattccttgg aaagtaacct gacgacattc cgcgtgatct acgccgacac ggacgcgatg
     1741 ggcattgtct atcacaccaa ctatatccgc tggtttgagc tgggccggaa tgaattgatg
     1801 cggcaaatgg ggattgtcta ttcggatctg gaaaacctgg ggctccatct gccattgacg
     1861 acggtgttct gccgctatct ccagccggcc agatacgacg acctgctcac ggtggagact
     1921 tgctttgact atatcaagag ggccagcatc cgttacagct ccaagatctg ggacgaaaat
     1981 cagcgaaaac tgctcgtgga aggatacacc atccatgcct gcaccagcaa cgaaggaaaa
     2041 atccggagga tgccggcatt gatcaccggg ttaatcgaga aatattctat tcgcaaaggg
     2101 gagtaaaatg gccgccaagt tatccaagaa agagctgaaa agcccggacg cttttcaaac
     2161 cacctttgac agagtgggcg agtacgtcga ggaaaataaa agccgcgtga tcatcgccgc
     2221 cgcggccctg tcgtgcgccg tgctgctggt cctgggcatt tatttttact ggagctatta
     2281 ttccggttcc gcgctgaaat tatacgccaa agcacaggac aatatcctga agggcggcga
     2341 caatccggaa acggaaaagg ccaacatccc gatcctgcag aagctggcgg atcagtaccc
     2401 ccacagctgg agcggccgga tggctcatta ccatcttgcc aatatttact acaaccaggg
     2461 agacctggac cgggcgatcg cttcctacga aacattcgtc gccaaaacag gatcggataa
     2521 aacgggcgtg agatttctgg cgctgacgtc tctgggctac gcttatgaag ccaggaagga
     2581 tttcagggcg gctctcaaat attttgagca ggcgcaaaaa ggttaccata ccggctttga
     2641 aatgatgagt ctgcgcaacg tggcgcgcgc tcacgaagcg ctgaacgaca aggccaaggc
     2701 aatggagtac taccaaaagg ctctggaaaa aacaacggat tcggcggccc aactctatct
     2761 tcgaagaaaa atagccgccc tgggctgagt tcccgcggcg ggtcccggac cgccatcggt
     2821 tatggaggag gcgcaagcag ttgaaaaaag aattgatgtc tggaaacgaa gccatcgccc
     2881 gcggcgtgta cgaggcgggc gtcaggtttg cggctgccta tccgggcacg cccagcaccg
     2941 aaatcatgga gcagtttgcc cggtacgacg gtgtttacgc cgaatgggcg cccaatgaaa
     3001 aagtcgcggt ggaggtggcc atcggcgcgg ccctgggcgg cgcgaaagcc ctggcggtca
     3061 tgaagcacgt cggcgtgaac gtggccgccg atccgatttt tacagtaagc tacaccggaa
     3121 tcgagggcgc actggtcatc atcagcgccg atgacccgtc catgcacagc tcgcaaaacg
     3181 agcaggacaa ccgaaactac gccaagttcg ccaaaattcc catgctggag ccgtccgatc
     3241 cccaggaggc caaggagtat gtcaagcttg ccttcgccat cagcgaacaa ttcgacactc
     3301 cggtttttct gcgcacgact acgcgcgtct cccattccaa gtccgtggtc acgctcagcg
     3361 agccgcgggt tcccgaagac aaaaccgcgc ttgcgcatca tgcggcaaaa tacgtcatgg
     3421 ttcccatcaa cgcgcgcatc cgccgcgtgg aagtggaaaa gcgccagaaa gccctgcaga
     3481 agtttgcgga gacgtttccg gaaaataaaa tggaaatcaa cgatccggcg gtcggcatca
     3541 ttaccgcggg catgccatac aactacgcga aggacgtttt tccggattat tcctatctga
     3601 agctgggcat ggtctatccg ctgccggtga aaatgatccg gcatttcgcc tccagggtca
     3661 aaaagattta tgtcatcgag gagctcgatc cctatctgga agagcagatc a
//
LOCUS       DAOSIM010000208         3696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_21946_length_3696_cov_10.132656, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000208 DAOSIM010000000
VERSION     DAOSIM010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21946_length_3696_cov_10.132656"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            358..807
                     /locus_tag="PLR47_08580"
     CDS             358..807
                     /locus_tag="PLR47_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOG13041.1"
                     /translation="MKIRDMLKATGFKSQSLITVGADEFVSAAIQKLIQHDKGALPVT
                     NEKDELIGIITERDIVRKCLARGGNCDDKKVGDIMTHEVAIASMEDDLDYAINTMRNK
                     KIRHIPIADGKKVVGMISMRDILGFQYEETKAEIKYIKLMPQRPVFK"
     gene            1087..1998
                     /gene="ftsY"
                     /locus_tag="PLR47_08585"
     CDS             1087..1998
                     /gene="ftsY"
                     /locus_tag="PLR47_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOG13042.1"
                     /translation="MGLFDKLKAGLTKTRDALTKNLDQLVFGKRELNPALYDELEDLM
                     ISADMGPQFTFDLIEDVKQRVKRKELSDASQIKKVLQERMIAILRKMETPLFINKNEA
                     FVIMAVGVNGSGKTTTIGKLAHMLRRDGHEVLLVAADTFRAAAIEQLEVWGTRVCAPV
                     IKQKMNADPAAVVFDAVGKIKAGFNGVVIVDTAGRLHTRTNLMEELKKVKRVIGKELP
                     GAPHEILLVLDATTGQNAVIQAKMFQEEIGATGIVLTKLDGTSKGGVVVRIAGELGLP
                     VRFIGVGEGLEDLRPFDSGDFTAALLD"
     gene            2025..2666
                     /locus_tag="PLR47_08590"
     CDS             2025..2666
                     /locus_tag="PLR47_08590"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOG13043.1"
                     /translation="MKIFAIGDIHGCLDQLQRLLKKIPADPEQDTLVFIGDFIDRGPE
                     SRGVVDCVLELCEIYAGMVCLCGNHESMLLDYLEGVEEELYLQNGGSTTLNDYGISLL
                     DPPRIRKAKIPADHLRFYESLLPYYETDQYLFVHAGLRPGIPRAEQSAQDMLWIRREF
                     IDSEADFGKRVVFGHTHFSAPLVAENKIGIDTGAVFGGRLTCAELPTLNFYQV"
     gene            2669..3589
                     /gene="ftcD"
                     /locus_tag="PLR47_08595"
     CDS             2669..3589
                     /gene="ftcD"
                     /locus_tag="PLR47_08595"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOG13044.1"
                     /translation="MKIIECVPNFSEGCDQAGIESIAGVLKAFPAIRLIDYSGDSDHN
                     RSVFTFLGKPEDVLEAALAASGQALKLIDMRKQTGVHPRLGAVDVVPFIPLGRTKMED
                     AVDLAHLFGRQLFERFGVPVYFYGHAALVPERRELANVRRGGYEALSDKMSNPGDAPD
                     AGKPVFNPRAGATAVGARELLVAYNINLNCDDLRLAQKIASLIREKSGGFPHVRAIGV
                     ILKSRRLAQVSMNLTNCKETPFKAVYERVEALAAERGVEILESELIGLAPKCAFAGTK
                     PQKLKLANFSKDRLLETHLKDFKDAESAGG"
BASE COUNT          859 a          961 c         1095 g          781 t
ORIGIN      
        1 gagcacgatg gaagtcgccg acacgctctt cggcgtgacc atgcaaaagc agggaatatc
       61 gtccctggta tccgtcaccc tcaattaatt cctttcctca gcccgcaatg ctaaaaaccc
      121 gtcggcagga cggcgtgcgg cgaacgtgcg agagcttttc aggaggccct gcgtttgcgc
      181 ttcggcgcgg tgcggaagaa aatgcttgat ttcagggaaa aatgagtgat atgaaaaaca
      241 agaaattccg ggagccggcg atggtttctg tgccggcata aaacattttc attcagaaaa
      301 cgcttcttaa cctgctgcag agcaactttt ttctattgga aaagggagga caatgaaatg
      361 aaaatacggg acatgttgaa agccacggga ttcaaaagtc aatcgttaat taccgtcggt
      421 gcggatgaat ttgtttccgc ggcgatccag aagctgattc agcacgataa aggggcgctc
      481 cccgtcacga atgaaaagga tgagctgatc ggcatcatta cggaaagaga catcgtgcgc
      541 aagtgcctcg cgcgcggggg gaattgcgac gacaagaagg tcggagacat catgacccac
      601 gaggtcgcca ttgcttcgat ggaagacgat ctggattacg cgatcaacac gatgagaaat
      661 aaaaagattc ggcatattcc gatcgcggac ggcaaaaagg tggtgggcat gatttccatg
      721 cgggacatcc tgggatttca gtacgaggag accaaggcgg aaatcaagta catcaaactg
      781 atgccccagc ggccggtgtt caaatagaat tttgttcggg cccgccgccg ctgaatccgg
      841 ggcgggcccg tcctttatcc gccgccgttg ttcgaaatcc gggccctttc ttttcccgcg
      901 ggcggggatt caccgcccgg cctgctcgga attccgccct tcctttcaga aaatcatcca
      961 tttctttact ttggcgccga aagccgctat gaattccata aggccgcgac ggttttcccc
     1021 tgtcggccgc atgacggctg ttttgatttc ccgacgggga ggtttgcaga aaggaacaag
     1081 aagaaagtgg gtttattcga caaactcaaa gcggggctga ccaagacccg ggacgccctc
     1141 accaaaaatc tggatcagct cgttttcggc aaaagggaat tgaatccggc actttacgac
     1201 gagctggagg atctgatgat ttccgctgac atgggtccgc agtttacctt tgacctcatc
     1261 gaagacgtca agcagcgggt caagcgcaag gaactttccg acgcgtcaca gatcaaaaaa
     1321 gttttgcagg agcgaatgat cgccattctg cgcaagatgg aaaccccgct ttttatcaat
     1381 aaaaacgaag cttttgtcat catggccgtc ggggtaaacg gaagcggcaa gaccaccacc
     1441 atcggcaaac tggcccacat gctgcgccgg gacggccacg aggtcttgct ggttgccgcc
     1501 gacaccttcc gtgcggcggc catcgagcag ctggaggtct gggggacccg cgtctgtgcg
     1561 cccgtgatca agcagaaaat gaatgcggat cccgccgccg ttgttttcga cgccgtggga
     1621 aagattaaag ccggtttcaa cggcgtggtg atcgtggata ccgccggccg cctgcacacc
     1681 cggaccaatc tgatggaaga gctcaaaaag gtcaaaaggg tcatcggcaa agagctgccc
     1741 ggggctccgc atgagatcct gctggttctg gacgccacga cgggccagaa tgccgtcatc
     1801 caggccaaaa tgtttcagga ggaaatcggg gcgaccggca tcgtgctgac caagctggac
     1861 gggacttcca agggtggagt ggtggtgcgc atcgccggcg aactgggtct tccggtgcgc
     1921 tttatcggcg tcggcgaggg gctggaggat ctgcggccct ttgacagcgg agattttacg
     1981 gccgcccttc tggattaggc ggccgctcat gaaggcggcg gatcatgaaa atattcgcga
     2041 tcggcgacat tcacggctgc ctggatcagc ttcagcgcct tctcaagaaa attccggccg
     2101 atccggaaca ggacacgctg gtttttatcg gcgatttcat tgaccgaggc ccggaaagca
     2161 gaggcgtggt tgattgcgtt ttggagcttt gtgaaatcta tgccggcatg gtctgccttt
     2221 gcggcaatca tgaatccatg ctcctggact atctggaagg cgtggaggag gagttgtact
     2281 tgcaaaacgg cggttccacg acgctcaatg actacgggat atctcttttg gatcccccgc
     2341 ggatcagaaa ggcaaaaata cccgctgatc atctgcgttt ttatgaatcg cttctgccgt
     2401 attatgaaac ggaccaatat ctgtttgttc acgccggcct gcgtccgggg atacctcgcg
     2461 ccgagcagtc cgcacaggat atgctgtgga ttcggcggga atttatcgat tccgaagccg
     2521 atttcggaaa aagggtggtt ttcggacata cccatttcag cgcgcctctg gttgccgaaa
     2581 acaagatcgg gattgacacg ggcgcggttt tcggcggcag gctcacctgc gcggagctgc
     2641 cgactttaaa tttttatcag gtgtaaatat gaaaattatc gaatgtgtgc cgaatttcag
     2701 cgaaggatgc gatcaggccg gaatcgaatc gattgccggc gtcttaaagg cgtttcccgc
     2761 catccgcctg attgactaca gcggggacag cgatcataac cggagcgtct tcacgtttct
     2821 gggaaagcct gaagacgtgc tcgaagcggc gctggccgca tcgggtcagg cgctgaaact
     2881 gattgacatg cgcaagcaga ccggcgtcca tccgcgcctg ggggccgttg acgtggtgcc
     2941 cttcatcccc ctggggagaa caaaaatgga agatgcggtt gatctggcgc acctctttgg
     3001 ccggcagtta tttgaacgat tcggcgtgcc ggtttatttt tacggccacg cggcgctggt
     3061 tcccgagcgc agggagctgg ccaatgtccg tcgcggcggg tacgaggcgc tgtcggacaa
     3121 gatgtccaac cccggcgacg cgccggatgc cggcaagccc gtctttaatc cgcgcgcggg
     3181 cgcaaccgcg gtcggcgcgc gcgaactcct ggtggcgtat aacatcaatt tgaattgcga
     3241 cgatttgcgc ctggcgcaaa aaatcgcatc actcattcgg gaaaaaagcg gcggatttcc
     3301 gcatgtgcgc gccatcggcg tcattttaaa aagcaggcgt ctggcgcagg tctccatgaa
     3361 cctgaccaac tgcaaagaaa caccgtttaa agcggtttat gaacgggtgg aggccctggc
     3421 cgccgaacgg ggcgttgaga ttctggagtc ggagttgatc ggactggcgc ccaaatgcgc
     3481 ttttgcgggc acgaagccgc aaaagctgaa actggcaaac ttttcgaaag acaggctttt
     3541 ggaaacgcat ctgaaggatt tcaaggacgc ggaaagcgcc ggaggttgac agtccgcgca
     3601 aagaatgtta aatcgccggc tccttcggga actccagcca aggctgcccc tgtagctcag
     3661 caggatagag cgacagattc ctaatctgtg ggccgc
//
LOCUS       DAOSIM010000209         3696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_21947_length_3696_cov_8.784125, whole genome shotgun sequence.
ACCESSION   DAOSIM010000209 DAOSIM010000000
VERSION     DAOSIM010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21947_length_3696_cov_8.784125"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..537
                     /locus_tag="PLR47_08600"
     CDS             94..537
                     /locus_tag="PLR47_08600"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOG13045.1"
                     /translation="MKNMMIAFMALLLVASFLPAGHAADPSITGVWSLPVLKGKDKGK
                     ERFQVEIFEKDGVYYGKIVKLTFLPENALCTKCKKEKKDKPLMGMLMLWNLKKEAGRY
                     VEGKIYDVEAGKEYKCSIAQITPDRLKITASLLFLSESHYWTRVK"
     gene            638..1072
                     /locus_tag="PLR47_08605"
     CDS             638..1072
                     /locus_tag="PLR47_08605"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOG13046.1"
                     /translation="MRMKFGFILGIIAVAIFATTSLWAADPIVGKWKTIDDETKQTKS
                     IVEIYLQDGKAFGKIAQLLRPEDKGKLCTKCTGADKDKPTEGLVIVKNMKDEGDQWAG
                     GTILDPAKGKEYKCKIKAIEGGNKLQVKGCIAFVCRTQIWVK"
     gene            complement(1317..3014)
                     /locus_tag="PLR47_08610"
     CDS             complement(1317..3014)
                     /locus_tag="PLR47_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOG13047.1"
                     /translation="MKFLSAIISTISGSKIVKANIGLLAKYLLVIIVLITAYSIVFHY
                     LMDLEGQRHSWMTGFYWTLVVMSTLGFGDITFTSDIGRAFSVLVLLSGVILLLILLPF
                     VFIKFFFAPWMEAQARSRAPRELPPDTRNHVIITHYDPVTADLIEKLVSYKKDYVVVV
                     DELQRALQLYDKSIRVAVGNIDDPKTYSRLRAQNAALVVATNSDQMNTNITLTLREDF
                     EHLPIVTTAESPHSVDILELAGSSRVLQLHDLLGGSLAAWTIAGDCKSNMIGRYDQLV
                     IAQAPVIGTPLVGKTLAESGIRETFGLAVVGIWERGTFIIPRPNTVIERTSVLVFAGT
                     EAAVAAYDEVYSFYQICKVAGDPVIIVGGGRVGHAIALRFQKADIPYLIIERAKKRSE
                     EDEHFVFGDAADIHTLQKAWFEKAPAALITSHDDATNIYLTKYLRSLRPDMQILSRAS
                     EDRNVSTLHRAGADFVMSYASLGANAIFNFLMNEETMFLAEGLNFFHLPAPRSLIGKA
                     LAQSGIREKTGCTVVAIQHRGETVINPEPSHAIDKDDELILIGTYDAESSFLGKFPA"
     gene            complement(3119..>3696)
                     /locus_tag="PLR47_08615"
     CDS             complement(3119..>3696)
                     /locus_tag="PLR47_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HOG13048.1"
                     /translation="FFSTSAVGYYGFHEDEELTESSPAGGDFLARLASDWEAEALRAK
                     DKGARVVITRFGIVLGPNGGALGQMIPLFKFFLGGPLGSGRQWFSWVHLQDLTAAFLF
                     LIGHPEISGAVNLCAPQPVRNRELGNAIGRVLHRPSFLPAPGFMIEIILGEFGSVLLK
                     GQRVIPRRLLDAGFRFSYPEIDEALHSIIKP"
BASE COUNT          899 a          924 c         1089 g          784 t
ORIGIN      
        1 ccccaatttt ccccttgcgt gtagatagca ttgctcatat actcgcgcgc ggtaaagcga
       61 tggcttgcgg gattttattg gaggaggaca cgtatgaaaa acatgatgat agcttttatg
      121 gcgcttcttt tggtcgcatc gtttttaccg gcgggtcatg cggcggatcc gtcgattacc
      181 ggtgtgtggt ctctgccggt tctgaagggc aaggacaagg gcaaggagcg gtttcaggtt
      241 gaaatctttg aaaaagacgg cgtgtattac ggcaaaattg taaaattgac ttttctgccc
      301 gaaaacgcgc tttgcaccaa atgcaagaaa gaaaaaaaag acaagccgct gatggggatg
      361 ctgatgttat ggaatctgaa aaaagaagcc ggccggtacg tcgaaggaaa gatttatgac
      421 gtcgaggcag ggaaggaata taaatgcagc atcgcgcaaa tcaccccgga caggctgaaa
      481 attacggcca gtttactttt tttgagcgaa agccactact ggaccagggt gaagtaagtg
      541 ttcctgattt ctagatcggg taattttagg aggcttgaca cggggactta atttgtgtag
      601 atttcgaaac caaaataaaa aatggaggtt tatagaaatg agaatgaaat tcggatttat
      661 attagggatc atcgctgttg ccatttttgc taccactagt ctctgggccg ccgatccgat
      721 cgtgggaaag tggaaaacca tcgatgatga aacaaagcag acgaaatcca tcgtagaaat
      781 ttaccttcag gatggaaagg catttggcaa aatcgcacag cttctgcgtc ccgaagacaa
      841 aggaaaactc tgtaccaagt gcacaggcgc tgacaaggat aaacccactg aaggcctagt
      901 tattgtaaaa aacatgaagg atgaaggaga tcaatgggcc ggtggaacga ttttagaccc
      961 agccaagggc aaagaataca aatgcaagat aaaggccatt gaaggcggga acaagctgca
     1021 ggttaaaggc tgcattgcct ttgtatgccg cacacagatc tgggttaaat agttcttaat
     1081 ggaaccggga tgattcttta ggattggcag aagaatagaa ttactttcgt ttcccggatc
     1141 aagaacagca tgatgtatta tagaatccct tcttgcacac cgaaaggaac gcaggaaggg
     1201 attttctatt tgaaaattac gattcatgga ggagcatctc cggatcaaat gtagaagttt
     1261 gacgctgatt tttcctgcaa ccacgccgtg cgaagaaggc cgacgctggc tgaagatcag
     1321 gcgggaaatt tccccagaaa ggagctctcc gcgtcgtaag tgccgatgag aatgagctcg
     1381 tcgtccttgt cgatggcgtg cgacggctcc gggttgatga cggtttcgcc cctgtgctga
     1441 atggcgacca cagtgcaccc ggttttttcc cgaatgccgg actgcgccag cgccttcccg
     1501 atcagggacc tgggcgccgg cagatggaag aaattcagcc cctccgccag aaacatcgtt
     1561 tcctcattca tcagaaaatt gaagatggcg ttggcgccga gcgaggcgta ggacatgacg
     1621 aagtccgccc ccgcgcggtg cagcgtggag acgttgcggt cctcgctggc gcggctcaga
     1681 atctgcatgt ccggccgaag gcttctcaga tatttggtga gataaatatt ggtggcgtcg
     1741 tcgtgcgagg tgatgagcgc cgcgggagct ttttcaaacc aggccttctg cagggtgtga
     1801 atatccgccg cgtccccgaa aacaaaatgc tcgtcctcct cgctgcgctt tttggcccgt
     1861 tcgataatca ggtacggaat gtccgccttt tgaaagcgca aggcgatggc atggccgacg
     1921 cgtcctccgc cgacaatgat gaccgggtcg cccgccacct tgcagatctg atagaaggaa
     1981 tacacctcgt cgtaggccgc cacggcggct tccgtgccgg cgaacaccag aacgctggtg
     2041 cgttcgatga ccgtgttcgg ccggggtatg atgaaggtgc cccgctccca gatgcccacc
     2101 accgccaggc cgaaggtttc acggattccg ctttcagcca gtgttttgcc gaccagcggt
     2161 gtgccgatga ccggcgcctg cgcgatcacc aactggtcat agcggccgat catgttggat
     2221 ttgcagtctc ccgcgatagt ccaggcggcc agcgaacccc ccagcagatc gtgaagctgc
     2281 aggacgcgcg aacttcccgc cagttcaagg atgtccacgg aatggggaga ctccgccgtg
     2341 gtgacaatcg gcagatgctc gaagtcttcc cgcagcgtga gggtgatgtt ggtgttcatc
     2401 tggtcgctgt tggtggccac gaccagcgcg gcgttctggg cgcgcagtct tgagtaggtt
     2461 ttggggtcgt cgatattgcc gacggcgacg cggatgcttt tgtcgtagag ctgaagcgcc
     2521 cgctgcagct catccacgac gacaacgtag tccttcttgt agcttaccag tttttcgatc
     2581 aggtcggcgg tcaccgggtc ataatgcgta atgatcacat ggttgcgggt atccggggga
     2641 agttcccggg gcgcgcggct tcgcgcctgc gcctccatcc agggggcgaa aaaaaacttg
     2701 atgaagacaa agggaagaag gatcagcagc agaatcacgc cggaaagaag caccaggacg
     2761 gaaaaggccc ggccgatgtc gctggtgaag gtgatgtctc cgaaccccag cgtggacatg
     2821 accaccaacg tccagtagaa gcccgtcatc caggaatgcc gctggccttc cagatccatg
     2881 agatagtgaa agacaatgct gtaggcggtg atcagcacga tgatgaccag aaggtacttt
     2941 gccaaaaggc cgatattggc tttgacgatt ttgcttccgg agatggtgct gataatggcc
     3001 gacaaaaatt tcacgagaga agcctctggg tttttcgctg atggctccag tatatgaagc
     3061 gcagcggggc ctgtcaatca aaatcagtcc gcttctgaag cgccgcgcgg cggctcatct
     3121 atggtttgat gatgctgtgc agcgcttcgt cgatctccgg ataggaaaag cgaaaccccg
     3181 catccagcag gcggcggggg atgacccgct ggcctttgag cagcaccgag ccgaactcgc
     3241 ccaaaataat ttcaatcatg aagccggggg cgggcaggaa ggaggggcgg tgcagaacgc
     3301 ggccgatggc gtttcccagt tcccggtttc ttacgggctg gggcgcgcac aggttcaccg
     3361 cgccggagat ttccggatgc ccaatgagaa agagaaatgc ggccgtgagg tcctgcaggt
     3421 gcacccatga aaaccactgg cgtcccgaac ccagcgggcc tcccaggaag aacttgaaca
     3481 gagggatcat ctgacccagc gcgcctccgt tggggccgag cacgatgccg aagcgggtga
     3541 tgaccacgcg agcgcctttg tctttggcgc gcagggcttc tgcttcccag tcggaagcca
     3601 gacgggccag aaagtcgccg ccggctggag aggattcggt aagctcctcg tcttcatgaa
     3661 acccgtaata cccgaccgcc gaagtgctga aaaaca
//
LOCUS       DAOSIM010000210         3619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_22546_length_3619_cov_7.080808, whole genome shotgun sequence.
ACCESSION   DAOSIM010000210 DAOSIM010000000
VERSION     DAOSIM010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22546_length_3619_cov_7.080808"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PLR47_08620"
     CDS             complement(<1..692)
                     /locus_tag="PLR47_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOG13049.1"
                     /translation="MTPSSADIIIRRVRAEGRRSLTEMESKKLIASYGVPVARDRMAA
                     SEREAVLQAQKTGYPVVVKGHGAKLQHKTERGLVKANLKSAKEVREAFRAIRASAGAD
                     GEGCIVSPQIAGSREWVAGLIRDVQFGPVVMFGLGGIFTEALADTAFRIAPLDRREAK
                     RMIGEISSRKLLGNFRGEAPVNADQLADVLLGLSRLGLERPDIREVDVNPLITTPKGN
                     VVAVDALVILDE"
     gene            910..1353
                     /locus_tag="PLR47_08625"
     CDS             910..1353
                     /locus_tag="PLR47_08625"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOG13050.1"
                     /translation="MMDWSFSPALVWAVVGLVLLIAELMAVSFILCFIGLGALVVALT
                     AWLGLTPSFSSQLIVFTVSSLLLMALLRKTARKLFAGQADAPPDYAGQRVPVVGAIPP
                     GGEGTIQYRGSVWIAFSDAAETIPEGAVVQIEAVEGIRVKVKPAA"
     gene            1373..2281
                     /locus_tag="PLR47_08630"
     CDS             1373..2281
                     /locus_tag="PLR47_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HOG13051.1"
                     /translation="MEVVLIVMALLAVVFIVKGVCIIPQQSAYVIERLGKFNRVLHAG
                     ISYIIPFVDRKAYVHTLKEQALDIPEQICITRDNVQVGVDGVIFIQVFDPQMASYGIS
                     NYVFSIVQLAQTTMRSEVGKIDLDKTFEERTTINSAIVSAINDASRTWGVKILRYEIK
                     NIRPPENVLNAMEKQMQAEREKRAVILQSEGEKQSAVNVAEGQKQKVVLESEAVRQKQ
                     INEATGQAEAIRAIASATADGLKAVAGAVQTEGGMEAVQLRVAEKLVEQFGHLAKQGN
                     TLILPANFGDISSLIATAMSVVKQTK"
     gene            2636..>2819
                     /locus_tag="PLR47_08635"
     CDS             2636..>2819
                     /locus_tag="PLR47_08635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13052.1"
                     /translation="MNLEKKTKKELVDLVTSLQKRLSVQEHTQLNVTSEKDLYKILAY
                     SSQVGCYIMQDGQFQFI"
     assembly_gap    2820..2829
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2830..>3619
                     /locus_tag="PLR47_08640"
     CDS             <2830..>3619
                     /locus_tag="PLR47_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG13053.1"
                     /translation="YTGYPEKKLLKMSPPSLILPEDRRRTAKNAIMMLKGQRFSPYEF
                     RIVTKDGRIKWIMETAMSITFKGRRAILGNSMDITERKEAGRRLEELEALESSILDAI
                     PQAVVGLHERRINFANHAVKMIFGWHREELIGKSIRLIYRNEEEADKIGEIFYRKLEH
                     QRTYETEFPCRHKDGQEMLCRMKASRIGEKLREKRVVVTYEDITQEKKAQEELERSRE
                     ELRNLSIHLQSVREEECARVARKIHDELGQSLTALQMDLSWLLNR"
BASE COUNT          800 a         1062 c          995 g          752 t
ORIGIN      
        1 ttttcatcca gaatcaccag cgcatccacc gccacgacat tccccttcgg agttgtgatc
       61 agcggattga catccacctc ccgaatgtcc ggccgctcca gtccaagtct cgacagaccg
      121 agcaaaacat cggccagttg atcggcatta acgggcgcct cgccgcgaaa attccccagc
      181 agctttcggg acgatatttc cccgatcatt cgttttgcct cccgccggtc caaaggagca
      241 atgcggaaag cggtgtcggc cagcgcttcg gtaaaaattc cgccgaggcc gaacatcacc
      301 accggcccga actggacgtc ccgaatcagg ccggcaaccc attcccggct gcccgcgatc
      361 tgcggagaaa cgatgcaccc ctccccgtcg gcgcccgcgg acgcccgaat ggcgcggaac
      421 gcttcccgca cttcttttgc ggatttcagg ttggctttga ccaggccgcg ttcggtttta
      481 tgctgaagct tcgcgccgtg ccccttgacg accaccggat agcctgtctt ttgcgcctgc
      541 aaaaccgctt cccgctcgct tgcggccatc cggtcgcgcg caacgggaac tccataagat
      601 gcaatcagtt ttttggattc catttccgtc agggaccgac ggccttcggc tctcacacgg
      661 cgaatgataa tgtccgcgct tgatggggtc atatttttcc ctcgaagttt tattgtgaac
      721 agggcatgaa ccgttaacac aaggccggac gttagtcaac ccggaggccg cttcgtccgg
      781 cgcaatactt cccgcgtggg cagatggcaa gcggacattt tcactttggc aaaaatagga
      841 catttctaaa atggcttgac tacaatcccc gttctttcta tacttcaata aacagaaagg
      901 cttgccgtca tgatggattg gtcattttct cccgcgctgg tctgggcggt cgtcgggctc
      961 gtgctcctca tcgcggagct gatggccgtg agcttcattc tctgcttcat cggcctgggt
     1021 gcgctcgtcg tcgcgcttac cgcctggctg ggactcacgc cttccttcag cagccagttg
     1081 atcgtcttta cggtttcatc cctcctgctg atggcgctgc ttcgaaaaac cgcccggaaa
     1141 ctcttcgcgg gccaggccga cgctccgccg gattacgcgg ggcaaagagt tcccgtcgtc
     1201 ggggctattc ccccgggcgg cgagggaacg attcagtacc ggggctcggt ctggatcgcc
     1261 ttttccgacg cggccgaaac gataccggaa ggcgcggtcg tgcagattga agccgtcgag
     1321 ggcatccgcg tcaaagtcaa acccgcagca taaacaaaag gagggaagaa tcatggaagt
     1381 tgtcctgatt gttatggcgc tcttggccgt ggttttcatc gtcaagggcg tttgcattat
     1441 tccgcagcag agcgcctacg tcatcgagcg gctggggaaa tttaaccggg tgcttcatgc
     1501 gggcatcagt tacatcatcc ccttcgtgga ccgcaaagct tatgtgcata cgctcaaaga
     1561 gcaggctctc gacatcccgg agcagatctg catcacgcgc gacaatgtcc aggttggcgt
     1621 ggacggcgtg atcttcattc aggtcttcga tccgcaaatg gcctcgtacg ggatcagcaa
     1681 ttacgtgttt tccatcgtgc agctggcgca gaccaccatg cgcagcgaag tcggcaagat
     1741 cgatctggat aaaacgtttg aagagcgaac caccatcaat tccgccatcg tctccgccat
     1801 caatgacgcc tcccggacct ggggcgtgaa aatcctgcgc tacgaaatca aaaacatccg
     1861 gcctccggaa aacgtgctga atgccatgga aaaacagatg caggccgaac gggaaaagcg
     1921 cgcggtgatt ctgcagtcgg aaggcgagaa gcagtcggcc gtcaatgtgg ccgagggcca
     1981 gaaacagaaa gtcgtgctgg aatcggaagc cgtgcgccag aagcagatca atgaggcgac
     2041 cggccaggcg gaagccatcc gcgcgatcgc ctccgccacg gcggacggtc tgaaagcggt
     2101 ggccggcgcg gtacagaccg agggcggcat ggaggcggtg cagctgcgcg ttgcggaaaa
     2161 actggtcgag cagttcggcc atctggccaa gcagggaaat acgctcattc tgccggccaa
     2221 cttcggtgac atctcctcgc tgatcgccac cgccatgagc gtcgtcaagc aaacgaagta
     2281 aatcgtcatg gttggaccgg ctcaccatga cacttgttgg tcgtcgctcg tcgttcgtct
     2341 tcctataacc catcgcccgt agggaacggt cgcgactgtt ccctacattc caccattcac
     2401 tctcttgtca tcccgagccc gtcgaggaat gccttcgact tttcaccgct tcctcttatt
     2461 aaaccttaac cacttaaaac ttaaaacttt taccccttgc ccatcgccca tcgcctattg
     2521 cccattacct catacctgtc ttttcacttt ccactcatcg ctcgttacta aggggtttat
     2581 ttttccctcc aattgtgttt agatgccgca agttcagtcc aaaacaggca agcggatgaa
     2641 tctggagaaa aaaacaaaaa aagaactggt cgatctggtc acgtctttgc agaagcggct
     2701 gtcggtccag gagcacacgc agctgaatgt cacgtcggaa aaggacctgt acaagattct
     2761 ggcgtacagc tctcaggtcg gctgctacat catgcaggac ggacagtttc aattcatcan
     2821 nnnnnnnnnt tacacgggct atcccgaaaa aaagcttttg aagatgagcc ccccttcgct
     2881 cattctgccg gaagaccgtc gccgaacggc caaaaacgcg atcatgatgc tgaaggggca
     2941 gcgcttctcc ccctatgagt ttaggattgt cacgaaagac gggcggatca aatggatcat
     3001 ggaaaccgcc atgtccatca ccttcaaggg gcggcgggcc attctgggca attccatgga
     3061 tatcaccgag cgcaaggagg ccggccggcg tctggaggag cttgaagcgc tggaatcgtc
     3121 cattctcgac gcaattcccc aggccgtggt gggcctgcac gagcggcgca tcaatttcgc
     3181 caatcacgcc gtcaagatga tcttcggctg gcaccgggaa gagctgatcg gcaaaagcat
     3241 ccggctgatc taccgcaacg aggaagaagc ggataaaatc ggcgagattt tttacaggaa
     3301 gctggagcat cagagaacct atgaaacgga gtttccctgc cggcacaagg acgggcagga
     3361 aatgctctgc cgaatgaagg cctcccgcat cggggaaaag ctgcgcgaga agcgggtggt
     3421 ggtgacctac gaggacatca cgcaggagaa aaaggcgcag gaggagctgg agcgctcccg
     3481 cgaggagctg cgcaaccttt ccattcacct tcagtcggtg cgcgaggagg aatgcgcgcg
     3541 cgtcgcccgc aagattcacg acgaactggg ccagtcgctc accgcgctgc agatggacct
     3601 gtcctggctg ttgaaccgt
//
LOCUS       DAOSIM010000211         3616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_22564_length_3616_cov_10.361696, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000211 DAOSIM010000000
VERSION     DAOSIM010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22564_length_3616_cov_10.361696"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..720)
                     /locus_tag="PLR47_08645"
     CDS             complement(238..720)
                     /locus_tag="PLR47_08645"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOG13054.1"
                     /translation="MQVFAASDIVEVAIRIEENGINFYKFAEQIAKQEDAKKLFSQLA
                     QAEAAHKRTFEKFFAAMEKYNPPEQYAGEYSTYLRNYVDNNLIFTKEIMDRQLAKVTD
                     TVGAFEFAMQRELDSVLYYHEIKNLVPAAQHEAIDKIIEEERRHYTMLCDMKKRYENR
                     "
     gene            complement(802..1863)
                     /gene="corA"
                     /locus_tag="PLR47_08650"
     CDS             complement(802..1863)
                     /gene="corA"
                     /locus_tag="PLR47_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOG13055.1"
                     /translation="MTIAVKKRSKKAGLSPGALIHIGRDYEDSSGIRLMRYDEATLEE
                     KEIFSPTELAAEKKKPGVLWVHVDGLKDIRRLENIGRVFGLHPLILEDILSTDQRPKA
                     EDFTDYIFIVLKNIQPDAGPALHAEQISILLGQNFVLSFQEKAGTLFDPIRERIRADK
                     GRIRKAGADYLAHHLMDAVVDQYFAVLEDIDDKIELLEDDLVRKTTPDKLQSIHLLKR
                     ELILLRKSLWPLREAIGLLQRADSPLIADSDLIYFKDISDHIIAVIDTVDTFRDMLAG
                     MLDVYLSSASMRMNQVMKVLTIIATIFMPLTFLAGIYGMNFANMPELGWRWGYFAVLG
                     VMLTIALGMLMLFRRKKWI"
     gene            complement(1876..3327)
                     /locus_tag="PLR47_08655"
     CDS             complement(1876..3327)
                     /locus_tag="PLR47_08655"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOG13056.1"
                     /translation="MDLISTAASVVKNIRVQDILDMAIIAGMIFALLMWFKTRASRFV
                     LIGVLLLGGVYLAARFFQLYLTVIVLQGFFAILLFVLVVIFQEDLRGFFERIAMLGQL
                     RKQAPPLSRLEKTANIIAETAGNLAKKRVGALMVIQGNDPLDRHLTGGIPLNGAASEP
                     LLESLFDPHSPGHDGAVILKEDRVTLFGCHLPLSVNTARPGNMGLRHTAALGLTERSD
                     ALCVVVSEERGSISVSEGESLSVIPGASTLHEVLEKYYAKYRPAPQKRPLRAWLSENT
                     KEKLIAVGLAFVLWATFGYQRDTVRRDFYVPLEYKNMPARWHFSEPPVKEVKVTLQGP
                     EQAFRLMNADSLKLSLDLSALAQKKLEFSLNRDMVNAPPGLSIAEISPSKIRIYAARQ
                     VAAHLPVHVATVNEPPDDLALQKISVRPAALSVMLDPKINPGGLRLETETLDLQNITS
                     TGTFTLKVKPLSGAFFPDGKSPQVRVTVRVRKK"
BASE COUNT          783 a          975 c         1033 g          825 t
ORIGIN      
        1 tcgttccgca gatgaagctg gtgatccggg gattcgtgac aacggccagc accaccgtca
       61 gagatccgaa aaacagtgtg gaaacagcca gatacggagc aaagatcaac catttgtagg
      121 gttgatacaa aatacgcagt attttattca ccgttttctt tccaaatcaa aattcggagc
      181 atcagttgat cgaacggctg aatagggcct gggtccgaac ccgtacgggc gttttcgcta
      241 tcggttttcg tagcgttttt tcatgtcgca cagcatggtg tagtgccgtc tttcctcttc
      301 aatgattttg tcgatggctt catgctgggc cgccggaacc aggtttttga tttcatggta
      361 gtacaggacc gaatccagtt cgcgctgcat ggcaaactca aaggccccca ccgtgtccgt
      421 cactttcgcg agctgccggt ccatgatttc tttggtaaag atcaggttgt tgtccacgta
      481 gttgcgcaga taggtgctgt attctccggc atactgctcc ggtggattgt atttttccat
      541 ggcggcgaaa aacttttcaa acgtgcgctt gtgcgcggct tccgcctgcg cgagctggga
      601 gaagagcttt ttggcgtctt cctgcttggc gatctgttcg gcgaatttgt aaaaattgat
      661 tccattttcc tcaattctaa tcgccacttc gacgatgtcg cttgctgcga atacctgcat
      721 agggtttcct cctgaaagaa tatgatgacc gcttaaaact gaacatattt taacggctat
      781 tgcaaccttt ttggcgatgt ttcaaatcca ttttttcctg cggaacaaca taagcattcc
      841 cagggcgatc gtcaacatga cgcccaggac ggcaaagtag ccccatcgcc agccaagctc
      901 cggcatattg gcgaaattca tgccgtagat gcccgccaga aaagtcaaag gcatgaaaat
      961 ggtcgcgata atggtcagca ccttcatgac ctggttcatc cgcatgctgg cggaggacag
     1021 gtagacgtcc agcatgccgg ccagcatgtc ccggaaggtg tccaccgtgt cgatgacggc
     1081 gataatgtgg tcggaaatgt ccttgaaata aatcaggtcc gaatcggcaa tgagcgggga
     1141 atcagcccgc tgcaagagac cgatggcctc ccgaagcggc cagagcgact tgcgcagcaa
     1201 aatcagctct cttttcagca ggtggatcga ctgcagtttg tcgggcgtgg ttttcctgac
     1261 gaggtcatct tccaggagtt caattttgtc gtcgatgtcc tccagcacgg caaaatactg
     1321 atcgacgact gcgtccatca ggtgatgcgc caggtaatcc gcccctgcct tgcggattcg
     1381 tcccttgtcg gcgcggattc tttcacggat gggatcgaac agcgttcccg ctttttcctg
     1441 aaaggacaaa acgaaattct ggcccagcag gatgctgatc tgctccgcgt gcagggccgg
     1501 gccggcgtca ggctggatgt tttttaaaac gatgaaaata taatccgtga agtcttccgc
     1561 cttcggccgc tggtcggtgc tgagaatgtc ttccaggatc aggggatgaa ggccgaacac
     1621 gcggccgatg ttctccagac gccgtatatc cttcaggccg tcgacgtgga cccagaggac
     1681 gcccggtttt ttcttttccg cggccagttc cgtaggcgag aagatttcct tctcctccag
     1741 cgtggcttcg tcgtagcgca tcagccggat gccggatgaa tcctcatagt cgcggccgat
     1801 atggatcagg gctccggggc tcaacccggc ttttttcgat ctctttttca cggcgatggt
     1861 catggcgctg gctccttatt ttttcctgac gcggacggtg acccggacct gcggggattt
     1921 tccgtccggg aaaaaagcgc cggacaacgg cttgactttc agcgtgaaag tcccggtgga
     1981 agtgatgttt tgcagatcga gggtctccgt ttccagccga aggccgccgg gattgatctt
     2041 ggggtccagc atcacgctca gcgcggccgg ccgcacggat attttctgca gggccaggtc
     2101 atccggcggc tcgttgacgg tcgcaacgtg gaccggtaga tgcgcagcca cctgccgggc
     2161 cgcgtaaatc cggattttgg aggggctgat ctcggcaatc gacaaacccg gcggagcgtt
     2221 caccatgtcc cggttcaggg aaaattccag ttttttctga gccagcgccg acagatccag
     2281 cgaaagcttc agcgaatctg cattcatcag ccggaaggcc tgttcgggtc cctgcagcgt
     2341 taccttgacc tccttcaccg gaggttcgct gaaatgccat ctggccggca tatttttgta
     2401 ttcgagggga acgtaaaagt cacggcgcac cgtgtcccgc tgatagccga acgtggccca
     2461 caagacaaag gccaggccga cggcaatcaa tttttccttt gtgttttcgc tgagccaggc
     2521 ccgaagaggt cttttttgcg gcgcgggccg gtacttcgcg tagtattttt ccagaacctc
     2581 gtggagcgtc gaggcgccgg ggatgacgga aagcgactcc ccctcggaga cggagatgct
     2641 gccgcgctct tcggacacga caacacacag ggcatccgat cgttccgtga gccccagcgc
     2701 ggccgtgtgg cgcagcccca tgtttccggg ccgcgcggta ttgacggaca ggggcagatg
     2761 acagccgaac agggttacgc ggtcttcctt gaggatgacc gccccgtcgt ggcccggaga
     2821 atgaggatcg aaaaggctct ccagaagcgg ctcgctcgcg gcgccgttta agggaatgcc
     2881 gccggtcaaa tggcggtcca gcggatcgtt tccctggatg accatcaggg cgccgacgcg
     2941 ctttttggcg agattccccg ccgtctccgc gatgatgttc gcggtttttt ccagcctgga
     3001 caggggcggc gcctgtttgc gaagctggcc cagcatggcg atgcgctcaa aaaagccgcg
     3061 caggtcttcc tggaaaatca ccaccagcac aaacagcaaa atggcgaaaa atccctgaag
     3121 cacaatcacg gtgagataaa gctgaaaaaa ccgggcggcc aggtaaacgc cgcccaacag
     3181 caaaacgccg atcaggacaa agcgcgacgc cctggttttg aaccacatca gcagggcgaa
     3241 aatcatcccg gcgatgatcg ccatgtccag aatgtcctga acccgtatgt ttttgacgac
     3301 ggacgcggcg gtgctgatca aatccatggc ggcacgtttc cagttcgaga aaaatgactg
     3361 gctgtgtata gcggcatttg atgacggatg tcaaggatca aggcaaaaaa acgcctcccc
     3421 gaaaagggga ggcgaatggg cggggcaaac cgcgagaaac tccttttccc gaagcgtcgt
     3481 cggcaaatgc cgtcttcggg aaaagggctt cgcctttagt agtattttcc ggcgatgtag
     3541 tcgctgagct gattgatgag gaaatccttg ttggagatga tcagcttcag gacgtcgccg
     3601 atggaaatga tgccga
//
LOCUS       DAOSIM010000212         3616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_22565_length_3616_cov_9.158102, whole genome shotgun sequence.
ACCESSION   DAOSIM010000212 DAOSIM010000000
VERSION     DAOSIM010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22565_length_3616_cov_9.158102"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLR47_08660"
     CDS             <1..408
                     /locus_tag="PLR47_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13057.1"
                     /translation="PAADDVENVYLYNIDHLQNIVDENIKNRRREALKAEAIIEEEAA
                     NYVSWLKELEAVPTIVSLRSKAENIVRAEMEKASVWMQSLENADREKVEALVHGIVNK
                     VLHAPVAVMKEESAEFASQDIVAAARQLFRLDD"
     gene            590..1504
                     /gene="hemC"
                     /locus_tag="PLR47_08665"
     CDS             590..1504
                     /gene="hemC"
                     /locus_tag="PLR47_08665"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOG13058.1"
                     /translation="MNIKIGTRGSKLALVQTNSAVAALQKAAPEIEAEICVIRTSGDI
                     MQDVSLTQIGGQGVFVKEIEEALLAGRIDLAIHSMKDVPGETPEGLVFAAILPREDVR
                     DVLVTRGRVKFEYLPKGAKIGTGSQRRGAQLKSFMPDLEIVPLRGNIDTRLKKIETEN
                     LTGIVLAAAGVKRLGYSEAVTQFLPTEIMLPAVGQGALGLQVRKSDAELAGLCARLNH
                     AVTEAEVTAERAYLRALGGGCRLPIAAYGLMEGKRLTLEGLLAAPNGGEVLRDKVWGE
                     PGEAEAMGRKLAEMILEKGGRRLLELMG"
     gene            1506..3038
                     /gene="cobA"
                     /locus_tag="PLR47_08670"
     CDS             1506..3038
                     /gene="cobA"
                     /locus_tag="PLR47_08670"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOG13059.1"
                     /translation="MKKGKVYIIGAGPGDPGLITLRAVEALKLADAVVYDNLVNEELL
                     AYAPVGARRIYAGKQGGDHTLTQDKINDLLVREALDGRTVARLKGGDPFIFGRGGEEA
                     EELAASGIPFEVIPGVTSAIAVPAYAGIPLTHRGLTSTVAFITGHEDPTKEKSDIDWQ
                     ALAGIGTLVFLMGVKNLGQITDALILRGKSPETPAALIRRGTLPEQQTLTGTLANIEE
                     LARANQFKPPAILVVGKVVELRDTLGWFDSKPLFGLGVVVTRPERQADDLAKLLAAEG
                     ARPIAFPTIAIEPPSNWSELDAALEELESYQWIIFTSANGVHFFFERLRQKGKDMRDL
                     KGVKVCCIGPATARQLEARGIGVDLVPEEFIAEGILKSFVALNIQGQKILIPRAAKAR
                     DLLPEGLKKMGAEVVVATTYRTVNSGRKKEELAVRLAAGDVDVITFTSSSTVTNFVDI
                     MGKDFALPPSVRVACIGPVTAGTAAKAGFKIDILQNEYTMEGLVRALVDHFKHPEGKD
                     SP"
     gene            3035..>3616
                     /locus_tag="PLR47_08675"
     CDS             3035..>3616
                     /locus_tag="PLR47_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG13060.1"
                     /translation="MIGISKLYCGAVEPSDVLRYNRHSGSLPSHLLQFSADKKPVVVW
                     NMTRRCNLKCIHCYSNSADIDYPDELTTQEAKKMIDDLAAFGSPVLLFSGGEPLMRKD
                     LLELAKYATDKGMRAVISTNGTLITKDIAAELKKIGLSYVGVSLDGLQPTHDRFRGRK
                     GAFEKAIEGIRNCREAGIKVGLRFTVNRHNVADV"
BASE COUNT          800 a          985 c         1153 g          678 t
ORIGIN      
        1 ccggcggcgg acgacgttga aaacgtctat ctgtacaaca tcgaccacct gcagaacatt
       61 gtcgatgaga atatcaaaaa ccggcgtcgc gaggcgctga aagccgaagc gatcatcgag
      121 gaagaggcgg ccaattacgt aagctggctc aaggagctgg aggccgtgcc caccatcgtg
      181 tcgcttcgga gcaaggctga gaacatcgtg cgggcggaaa tggaaaaagc gtccgtctgg
      241 atgcaaagcc tggaaaacgc agaccgggaa aaagtcgaag ccctggtgca cggcattgtc
      301 aacaaggtgc tgcacgcgcc ggtcgcggtg atgaaagagg aaagcgccga atttgcctcc
      361 caggatatcg tggcggcggc caggcaactc tttagattgg atgattaatg cagggaacgg
      421 tttgaaacct ttccctgcga cttccctccc tcgaggggag ggattgaggg agggtgaatt
      481 tggccggaga aattgctgac agccgtcatg ttcgttggat attaaactta ccccctcccc
      541 ttaatcccct cccgcaaggg gcggggagaa aaaatgaatc gggagtttta tgaatattaa
      601 aatcggaaca agaggcagta aactggcgct ggtgcagacg aattccgcgg tggctgccct
      661 gcagaaagcg gcgccggaga tcgaggctga aatctgcgtc atcaggacca gcggcgatat
      721 tatgcaggac gtctctctga cgcagatcgg cgggcagggg gtttttgtaa aagaaattga
      781 agaggcgctt cttgccggtc ggattgacct ggcaatccat tccatgaaag acgttcccgg
      841 cgagactccg gaggggctcg tctttgcggc gattctgccg cgcgaggacg tccgcgatgt
      901 tctggtgacc cgcggcaggg tcaaatttga atatttgcca aagggggcta agatcgggac
      961 cggttcgcag cggcgcggcg cgcagttgaa atcgtttatg ccggatctgg aaattgttcc
     1021 gctgcggggc aacatcgaca cgcgcctgaa aaagatcgaa acggaaaatc tcaccggcat
     1081 tgttcttgcc gcggcgggcg tcaagcgtct gggctattca gaagcggtga cgcagtttct
     1141 gccgacggaa atcatgctgc cggccgtggg acagggcgct ctgggtctcc aggtgagaaa
     1201 aagtgatgct gagctggcgg ggctttgcgc gaggctcaac catgccgtga ccgaagcgga
     1261 agtgacggcg gagcgcgcct atctgcgggc gctgggcggc ggctgccgcc tgccgattgc
     1321 cgcttacggc ctcatggaag gcaaacggct gacgctggaa ggcctgctgg ccgcgcccaa
     1381 cggcggggaa gtgctgcgcg acaaggtctg gggcgagccg ggcgaagccg aagcgatggg
     1441 acgaaagctt gcggagatga ttctggaaaa aggcggccgc cgcctgctgg agttgatggg
     1501 gtaaaatgaa aaagggtaaa gtttatatta tcggtgcggg gccgggggac ccgggcctca
     1561 tcacgctcag ggcggtggag gccctgaagc ttgccgatgc ggtggtttac gacaatctgg
     1621 tcaatgaaga gcttctggcc tacgcgccgg tcggcgcacg cagaatatac gcgggcaagc
     1681 agggcgggga ccacacactg acgcaggaca aaatcaacga ccttctggtc cgggaagctc
     1741 tggacggccg cacagtggcg cgcctcaagg gcggcgatcc ttttatcttt gggcgcggcg
     1801 gcgaggaagc ggaagagctg gccgcttccg gcattccctt tgaagtgatt cccggcgtga
     1861 cctccgccat cgcggtgccg gcctatgcgg gcattccgct cacgcaccgg gggcttacct
     1921 ccacggtggc gtttattacc ggccacgaag atccgaccaa ggaaaaaagc gacatcgact
     1981 ggcaggcgct tgcgggcatc gggacgctgg tgtttctgat gggcgtgaaa aatcttggtc
     2041 agatcaccga cgcattgatc ttgcgcggaa agtctccgga gacaccggct gcgttgattc
     2101 gccggggcac gctgcccgaa cagcaaaccc ttacgggcac gctggcgaac attgaggaac
     2161 tggcccgcgc caaccaattc aagccgcccg ccattctggt ggtggggaag gtggtcgaat
     2221 tgcgcgatac gctggggtgg ttcgattcga agcccctctt cggcctgggc gtcgttgtca
     2281 cgcggcccga acggcaggcc gacgatctgg caaagctttt ggcggctgaa ggcgcacggc
     2341 cgatcgcgtt tccaaccatc gccatcgagc cgccttccaa ctggagtgaa ctcgacgcgg
     2401 ccctggagga actggagtcg taccagtgga tcattttcac cagcgccaac ggcgtgcatt
     2461 tctttttcga gcgcctgcgg caaaagggca aagacatgcg cgacctcaag ggtgtcaaag
     2521 tctgctgcat cggcccggct acggcaagac agttggaggc ccgcggcatc ggtgtggatc
     2581 tggtgccgga ggagttcatc gccgaaggta ttttaaaatc ctttgtcgcc ctgaacattc
     2641 aaggccaaaa aattctgatt ccccgcgcgg ccaaagcccg tgatcttctg cccgaaggcc
     2701 tcaaaaagat gggggccgaa gttgtcgtgg ccacaaccta ccggacggtg aattccggcc
     2761 ggaagaaaga ggaactggct gtgcggcttg cggcgggaga cgtggacgtc atcacgttta
     2821 ccagctcctc caccgtgacg aattttgtgg acatcatggg aaaggatttt gcgctgccgc
     2881 cctccgtccg tgtggcctgc atcggtcccg taacggccgg gaccgccgcg aaagcgggat
     2941 tcaaaatcga tatcctccag aacgaataca cgatggaggg tctggttcgg gcgctcgtcg
     3001 atcactttaa gcatccggaa ggaaaggaca gtccatgatc ggcatttcca aactgtactg
     3061 cggagcggtg gagccatccg acgtgctgcg ctacaaccgg cactcgggat cgctgccgtc
     3121 gcatctgctg caattttcgg cggacaaaaa acctgtggtg gtctggaaca tgacgcgccg
     3181 ctgcaacctc aaatgcattc actgctattc caactcggcc gacatcgact atcccgatga
     3241 gctgacgacc caagaagcca aaaaaatgat cgatgacctg gcggctttcg gatcaccggt
     3301 cctgctgttt tccggcggcg aaccgctgat gcgtaaggat ttgcttgaac tggcaaagta
     3361 cgcgacggac aagggcatgc gcgcggtcat ctctaccaac ggcacgctca ttaccaaaga
     3421 catcgccgcc gaactcaaaa aaatcggcct gtcgtatgtc ggcgtcagcc tcgacggttt
     3481 gcagccgacg catgaccgtt tccgcggcag aaaaggcgcg tttgaaaaag ccatcgaagg
     3541 cattcgcaac tgccgggaag caggcatcaa ggtgggcctg cgcttcaccg tcaaccgcca
     3601 caacgtggcc gatgtg
//
LOCUS       DAOSIM010000213         3603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_22661_length_3603_cov_7.001409, whole genome shotgun sequence.
ACCESSION   DAOSIM010000213 DAOSIM010000000
VERSION     DAOSIM010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22661_length_3603_cov_7.001409"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="PLR47_08680"
     CDS             <1..1216
                     /locus_tag="PLR47_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOG13061.1"
                     /translation="IGESDIQGKFSIDDFRKPAVAFQVKADRLHARDLGWTSPKGDAT
                     FSGVSAQAVWKGNDLLVNRLSFGLGQSLFNASGHLRDFAEPKITAELASPYIHSDDIA
                     LLASLKTSKKDGSAPSNITVDATVRANAGKLEGVEFSGLNTVLNYTTGILNVSSLEAG
                     VFDGRIRAKGKAALRPDGQNRYDANLSVNGVSLEKLQSIMDIGDGLITGSLSVSGDLT
                     AAGSQAADLKNTAAGTLQIRAEKGVLRKFSVLSKIFSLLNVSQLIRLKLPDMAKDGMP
                     YSAISAQASVQNGVFSSKDFFISGDAMEISAAGSVDVFRKELDGIAGIHPLQTLDLVA
                     SKMPIVGWLILDKNGNILTIYVKINGAWKDPNVKPMPVRSIGRETLGVFRRLFQLPEK
                     LITDTGEVILGN"
     gene            1227..2564
                     /locus_tag="PLR47_08685"
     CDS             1227..2564
                     /locus_tag="PLR47_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOG13062.1"
                     /translation="MAENKTRIQRTMDFIRKDIWRIRGTGLPPGKSFFLKLARVLILS
                     IRGFDEDKCQLRASSLTFYSLVSIVPIAAMAFGIAKGFGFQKVLEEQLREKLAGHEDI
                     LANVIQFSDALLANTKGGLIAGVGLVVLFWAVIKMLRQIEESFNDIWGIREKRTIGRM
                     FGDYLSLILICPVILVLSGGVNVFITTQVNLILEKFAILGSFGPVIFFLLKLLPYTLM
                     WALFTFLYIFMPNTQVKFSAGIIAGIITGTIFQVVEWAYITFQVGVAQYNAIYGSFAA
                     LPLFLAWLQLSWLIVLYGAELSFAYQNVDTYEFEPDALAASHRLRTLLSLRIAHHMVQ
                     RFVQGEKPATARDISNQLEIPIRFVNEILFNLVKSNILSMARTEGITEQGYQPARDVN
                     TLTVQYVIGAMDKRGLNQTPFTRSPEFDALSASLETFDQTIERQPENKLLRDL"
     gene            2566..3168
                     /locus_tag="PLR47_08690"
     CDS             2566..3168
                     /locus_tag="PLR47_08690"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HOG13063.1"
                     /translation="MIAKRSFLFRALLGITCLCWMEGCAPTHIVMDVESRAAPSFPAP
                     ACKKFSFLSPEGEMPLTENLLPIIRGELEKKGLVHDRAKPHVLVLIKAGTRAEQRQEP
                     VVSRPVPSLQPTPKGATMEMHYVIEGGGFYTVHIRWISMDVFDSAGRKPGDELWQGKV
                     ASEGEEDLDSVAKCLISGLLADYPAGPGKTRKILKLNECK"
     gene            complement(3152..>3603)
                     /locus_tag="PLR47_08695"
     CDS             complement(3152..>3603)
                     /locus_tag="PLR47_08695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG13064.1"
                     /translation="SLHMAMLIVGKVLTGSLADRLGLLKSYLVCMAGLVIAILLLYGA
                     QLMWVAVVFSVLFGFSIAVRTVLPPLMTAKVLGQKHFAVIYGLLNIFTTLGTAVGVPL
                     SGFIYDWTKSYSLAFALYIGLCLIAAAAGIAVMIRGEKKQSSVICIR"
BASE COUNT          848 a         1031 c          955 g          769 t
ORIGIN      
        1 aatcggagaa agcgacattc agggaaagtt cagcatcgat gatttccgca aaccggccgt
       61 cgcgtttcag gtcaaggctg atcggctcca tgccagggac ctcggatgga caagcccgaa
      121 aggcgacgca acgttttccg gcgtcagcgc acaggccgtc tggaaaggca atgaccttct
      181 cgtcaaccgc ctttctttcg gcctgggaca atctcttttc aacgcatcgg gtcacctccg
      241 ggactttgcc gagccgaaaa tcacggcgga actggcctct ccctacatcc attccgacga
      301 catcgctcta ctggcctccc tgaaaacgtc caaaaaagac ggaagcgcgc cgtccaacat
      361 aaccgttgac gcaaccgttc gcgcgaatgc gggaaagctg gaaggcgtcg aattcagcgg
      421 cctgaatact gtgctgaact atacgaccgg aatcctgaat gtctcatccc tggaagcggg
      481 cgtttttgac ggacgcattc gggcaaaagg caaagcggct cttcgtcctg atggtcagaa
      541 ccgctatgat gcaaaccttt ccgtcaacgg ggtgagtctg gaaaaactcc agagcatcat
      601 ggacatcgga gacggtctga tcaccggcag tctgtcggtt tccggagacc tgaccgctgc
      661 cggaagtcaa gccgccgatc tgaaaaatac agcggccgga acgctgcaaa tccgcgccga
      721 aaaaggcgtc ctgagaaagt tttcggtcct ttccaaaatc ttctccctgc tgaatgtttc
      781 gcagcttatc cggctgaaac tgccggacat ggccaaggac ggcatgcctt acagcgccat
      841 tagcgcccag gcctccgttc aaaacggcgt gttttcatcc aaagatttct ttatttccgg
      901 cgacgcaatg gaaatctcgg cggcgggcag cgttgacgtg tttcgaaaag agctggacgg
      961 catcgccggc attcaccccc tgcaaaccct ggatctggtt gcttcaaaaa tgccgattgt
     1021 aggctggctg attctggata aaaacgggaa tatcctcacc atttatgtta aaatcaacgg
     1081 cgcgtggaaa gacccgaatg tcaagcccat gccggtgaga tccatcggca gagaaaccct
     1141 gggcgttttc cgtcgtcttt tccaactgcc ggaaaagctg atcaccgaca cgggggaagt
     1201 cattctgggg aactaaagga ggcatcatgg ccgaaaataa aacacgaatt caaagaacca
     1261 tggatttcat ccggaaagac atctggagaa ttcgcggcac gggacttcct cccggaaaat
     1321 ctttttttct gaagctggcc agggtgctga ttctttccat ccgcggcttt gacgaagaca
     1381 aatgccagct tcgcgcatcc tcgctgacgt tttattcgct ggtctcgatt gtccccatcg
     1441 ccgccatggc cttcggcatc gccaaaggct tcggctttca gaaagtgctg gaagagcaac
     1501 tgcgggaaaa actggccggc catgaagata ttctggccaa tgtgatccag ttttccgacg
     1561 cccttctcgc caacacaaag ggcggcctga tcgccggcgt cggcctggtg gtgcttttct
     1621 gggccgtcat caaaatgctg aggcagatcg aagagtcttt caacgacatc tggggcattc
     1681 gggaaaaacg aaccatcggc cggatgttcg gcgattatct gtctctcatc ctgatctgcc
     1741 cggtcatcct ggttctatcg ggcggtgtga acgtttttat taccacgcag gtgaacctga
     1801 ttctggagaa attcgccatt ttgggaagct tcggccccgt catctttttt cttctgaagc
     1861 tgctgcccta taccctcatg tgggcgcttt tcaccttcct ctatattttc atgccgaaca
     1921 cccaggtaaa attttccgcc ggcattatcg cgggaatcat caccggaacg atctttcagg
     1981 tggtcgaatg ggcctatatt acgttccagg tcggcgttgc ccagtacaat gccatttacg
     2041 gtagcttcgc cgccctgccc cttttcctcg cctggcttca gttgagctgg ctgatcgtgc
     2101 tgtacggggc ggagctttcg tttgcctacc agaacgtgga cacttacgag tttgaaccgg
     2161 atgcgctcgc ggccagccac cgccttcgca cgctgctttc cctgcgcatc gcccatcata
     2221 tggtccagcg gttcgttcag ggagaaaaac cggcgacggc ccgagatata tccaatcagc
     2281 tggaaattcc cattcgcttc gtcaacgaaa ttctgttcaa tctggtgaaa agcaatattc
     2341 tttcgatggc ccggacggag ggaatcacgg agcagggtta tcagccggcg cgcgacgtca
     2401 atacgctgac cgtccagtat gtcatcggcg caatggacaa acggggcctg aaccagacgc
     2461 ctttcacgcg ctcccctgaa ttcgacgccc tttcggcttc cctggaaacg tttgatcaaa
     2521 cgatagagcg tcaaccggaa aacaagctgc tcagggattt gtagcatgat cgccaaaaga
     2581 tcatttttat tccgcgccct gctggggata acctgtctgt gctggatgga aggctgcgcc
     2641 ccgacgcata tcgtgatgga tgtcgaaagc cgcgcggcgc caagcttccc tgccccggct
     2701 tgcaaaaagt tttccttcct ttcacctgaa ggcgagatgc ccctgacgga aaacctgttg
     2761 cccatcatcc gcggcgagct ggaaaaaaaa ggcctggtgc atgaccgcgc caagcctcac
     2821 gtcctcgtcc tgatcaaagc cggcacaaga gccgagcaaa ggcaggagcc ggtcgtatcc
     2881 cgcccggtcc cgtctcttca acccacgcca aaaggcgcca caatggaaat gcactatgtc
     2941 atcgagggag gcggattcta cacggttcat attcgctgga tcagcatgga cgtcttcgac
     3001 tcggcaggcc gcaagccggg cgatgaactc tggcagggca aggtcgcaag cgagggagag
     3061 gaagacttgg acagcgtggc caaatgcctg atctccgggc tgcttgcaga ttatcctgcc
     3121 ggaccgggca aaactagaaa aattctcaaa ctcaacgaat gcaaatgacg gacgattgtt
     3181 ttttttcgcc ccggatcatg acggcaatgc cggctgcggc cgcgatcagg caaagcccta
     3241 tatacagagc aaatgccagg gaataactct ttgtccagtc gtaaataaag ccggaaagcg
     3301 gcacgcccac ggccgtcccc agcgttgtga agatgttgag cagtccgtag atgaccgcaa
     3361 aatgcttctg ccccaaaact ttcgccgtca tgagcggcgg caggacggtg cgcaccgcga
     3421 tggaaaaacc gaaaagaacg ctgaaaacga cagccaccca catcaactgc gccccgtaaa
     3481 ggaggagaat ggcaatcacc agtccggcca tgcagaccag atagcttttc agaaggccca
     3541 gccggtcagc caggctgccc gtcagcacct tgccgacaat cagcatggcc atgtgcaggg
     3601 aca
//
LOCUS       DAOSIM010000214         3596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_22704_length_3596_cov_7.385767, whole genome shotgun sequence.
ACCESSION   DAOSIM010000214 DAOSIM010000000
VERSION     DAOSIM010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22704_length_3596_cov_7.385767"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1374)
                     /gene="nrfD"
                     /locus_tag="PLR47_08700"
     CDS             complement(91..1374)
                     /gene="nrfD"
                     /locus_tag="PLR47_08700"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOG13065.1"
                     /translation="MGCPENSSWKGCVQDAVRRVFSGGPVYLFFLGLFAVLLVAGIAA
                     GIHSVYIVGSRHAYGSTREIPLAMLIATYTFFVITSTGLCLVSSIGHIFGVERFMPIA
                     KRAVLLSVITIMSGFLVIGLELENPLRLGIYLFLSPNFTSNIWWMGVLYSFEVVLLIL
                     EFIFILLDNHKASVAAGLLGILFGIAAISNLGGVFAMLNGREFWYGPYLPIFFIASAL
                     LMGCAAVIFFTVLAYRIQREPLESPMADALAAVGKLAGLMLAVIMFFTIWRMVALAVG
                     GTHELLVRDALVSGPFAFNFWVMEAGLGMILPFALVLWSAGKRIGPLFLASALVIFSS
                     FFMRLDMVVAGQIVPMYWELGVREYARLHEYSPTGHEILVVLAGVGLTGAAFLLGEKV
                     FQGFREVEGAGSGSGGTPEDVAQEPRSASKGKVDV"
     gene            complement(1412..1960)
                     /locus_tag="PLR47_08705"
     CDS             complement(1412..1960)
                     /locus_tag="PLR47_08705"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOG13066.1"
                     /translation="MKNRSILFAALMILLLCLYGQGSFAQGQRAKKAAPAKVVRLAGA
                     QAWDCAACHRTQKVLPKNHLDIQEMPYVGCLVCHLSEEGFAGSLKGKMPGSHLHAFRN
                     VQCAGCHGREKKPSAVAKSRCLDCHGSAAAVAGKTQNTKPNNPHDSPHYGTELDCNFC
                     HHQHAGSEDYCAKCHKFNFVVP"
     gene            complement(2144..2848)
                     /locus_tag="PLR47_08710"
     CDS             complement(2144..2848)
                     /locus_tag="PLR47_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG13067.1"
                     /translation="MLKLINVTTMIGEHQVIHDVSLQIARGDFVALLGGNGAGKTSLF
                     RTIVGVLPPAKGRIEFKGEEISHIAAHKIVGKGIALCPEGRQLFPQLSVQKNLMLGAY
                     HRKDKKGVEETLEQVYGLFPRLKERYKQMAGTFSGGEQQMLAIGRAIMSKPEMLLLDE
                     PSMGLAPLVVEGIADAITQLNQMGITVFLSEQNAQIALMITHRGYVLENGRLVLEGLS
                     RDLITNEKVKKAYLGT"
     gene            complement(2841..>3596)
                     /locus_tag="PLR47_08715"
     CDS             complement(2841..>3596)
                     /locus_tag="PLR47_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG13068.1"
                     /translation="KGENLVKNFGGTIAVNDVSFEVHEGEILSIIGPNGAGKSVLFSL
                     ISGAGKPTSGSIRLAGDEIAGQSASQISRKGIARTFQLTALFEKLPVAANLALGYYKR
                     TKSGFWGGILHTPQWKRDKKMVEEKVLQTLEFVGLGPRAFDIASTISQGEQRLLSIGI
                     ALISDPRLILFDEPTGGLIQEDTEMITRLIRKINQKGITVCLIEHKMRMIMDLADRIV
                     VLNYGRKIAEGKPADVAADPQVIEAYLGRKKDA"
BASE COUNT          782 a         1075 c          993 g          746 t
ORIGIN      
        1 cgattttcag aaattcccgg cggtccattt cggaaatctt ttccgggttt ttcagcatgg
       61 tttctgcgtc cagtgatttg aagatcatgg ttagacgtca acctttcctt ttgacgccga
      121 ccgcggctcc tgcgcgacgt cttcaggcgt cccgcctgaa ccggacccgg cgccctcaac
      181 ctctctgaat ccctgaaaaa ctttttctcc cagcagaaac gccgcgccgg taagtccgac
      241 gccggccagc accaccagaa tttcatgacc ggtgggcgaa tactcgtgga gtctggcata
      301 ctccctgacg ccgagttccc agtacatggg aacaatctgc cccgcaacca ccatatccag
      361 gcgcatgaaa aaggagctga atatcacgag ggcggaagcc agaaacaggg gcccgatccg
      421 cttgccggcg gaccagagga ccagcgcgaa gggaaggatc atgcccaggc cggcctccat
      481 cacccagaaa ttaaaggcga aggggccgct gaccagggcg tcgcgcacca aaagctcatg
      541 cgttcctccc accgccagcg caaccatccg ccagatcgtg aaaaacatga tcaccgccag
      601 catcagaccg gcaagttttc caaccgccgc cagagcgtcg gccatggggc tctccagcgg
      661 ttcacgctga atgcggtagg cgagaaccgt aaaaaaaatc acggcggcgc agcccatcag
      721 cagggcggag gcaatgaaga aaataggcag atagggtccg taccagaatt cccggccgtt
      781 gagcatggca aagacgcccc ccaggttgct gatggcggca atgccgaaga ggatccccag
      841 aaggcccgcc gccaccgacg ctttgtggtt gtccagcagg atgaagataa attcaaggat
      901 cagcaggacc acctcgaagg agtagagaac ccccatccac cagatattgg aggtgaagtt
      961 cggcgagaga aacaggtaga tgcccagccg cagcggattt tccagctcca ggccgatgac
     1021 gaggaatccc gacatgatgg tgatcacgga cagcagcacc gcccgcttcg cgatgggcat
     1081 gaaacgctcc acgccgaaga tgtgtccgat ggaggacacg aggcacaggc cggtcgaggt
     1141 gatgacaaaa aaggtatagg tggcgatgag catggccagc ggaatctccc gggtgctgcc
     1201 gtaggcgtgg cggcttccca cgatgtagac ggagtgaatt cccgcggcaa tccccgcgac
     1261 cagcagcacg gcaaacagac ccagaaaaaa cagatacacc gggccgccgg agaagacgcg
     1321 ccggactgcg tcctgaacac agcctttcca tgatgaattt tccggacatc ccatcgtcat
     1381 ttttccttat gccttcccgg gccggttcgt gttacggcac cacaaaatta aatttgtggc
     1441 actttgcgca ataatcctcc gacccggcat gctggtggtg gcaaaaatta cagtccagct
     1501 ccgtcccgta gtggggcgag tcgtgcggat tgttcggttt ggtgttttgt gtctttccgg
     1561 caaccgcggc agcgctgccg tggcagtcca gacaccggct tttggcgacc gcagacggct
     1621 ttttctcccg gccgtggcac ccggcgcatt gaacattccg gaaggcatgc aggtgactgc
     1681 cgggcatttt ccccttgagc gagccggcaa agccttcttc ggaaagatgg caaaccaggc
     1741 agccgacata cggcatttct tgaatgtcca ggtgattttt cggaagaact ttttgcgtgc
     1801 gatggcaggc cgcgcagtcc caggcctgcg cgccggcaag ccggacaacc ttggctggag
     1861 ctgctttctt cgcccgctgt ccctgggcaa agcttccctg tccgtacagg caaagaagga
     1921 gaatcatcag agcggcaaac aatattgatc ggttcttcac aaaacctcgc aagctcgcaa
     1981 gctgggtcat ttctaacaaa taagccggtt cgtgtcaaac caattcatgc tctgcggcaa
     2041 ccggcgcaac ctttcggaga caccgataaa aagagctctc cccccgaagg ggcaagagct
     2101 cttggaattc aggatttgag tccgatccgg cggctggaaa aattcaggtt cccaggtacg
     2161 cctttttcac cttttcattg gtgatcaggt ccctgctcaa tccctccagc acgagacggc
     2221 cgttttccag aacatagccc cggtgcgtga tcatcagggc tatctgcgcg ttctgctcgg
     2281 acaaaaagac ggtgatgccc atttgattga gctgcgtgat cgcgtcggcg atgccttcca
     2341 ccaccagcgg cgccagtccc atggagggct cgtccagaag gagcatctcc ggcttggaca
     2401 tgatggcccg cccgatggcc agcatctgct gctcgccgcc ggaaaaagtt ccggccattt
     2461 gtttgtagcg ctccttgagg cgcgggaaaa gcccgtaaac ctgctccagc gtctcttcaa
     2521 cgcccttctt gtccttgcgg tggtacgcgc ccagcatgag atttttctgg acggaaagct
     2581 gcggaaagag ctggcgcccc tcgggacaga gcgcgatgcc ctttcccacg attttatgcg
     2641 cggcgatgtg gctgatctcc tcgcctttga attcgattct gccttttgcc ggcggcaaaa
     2701 ctccgacaat ggtgcggaac aaactggttt tcccggcgcc gtttccgccc aacagcgcaa
     2761 caaagtctcc gcgggcaatc tgcagcgaaa catcgtggat cacctgatgc tcgccaatca
     2821 tcgtcgtgac atttattaac ttaagcatcc ttttttcttc ctaagtaggc ttcgatgacc
     2881 tgcgggtccg cggccacgtc cgccggcttg ccttccgcaa ttttccggcc gtagtttaac
     2941 acgacaatcc tgtccgccaa atccatgatc atccgcatct tgtgttcaat caggcaaacg
     3001 gtgatgccct tctggttgat cttgcggatc agccgcgtga tcatctccgt gtcttcctga
     3061 atcagaccgc ccgtcggttc gtcaaacaga atcaggcggg ggtcgctgat gagcgcgatg
     3121 ccgatggaga gcagccgctg ctccccctgg gaaatcgtgg aggcgatgtc gaaggctctg
     3181 gggcccaggc cgacgaattc aagggtctga agaacctttt cctccaccat ctttttgtcc
     3241 cgcttccatt gcggcgtgtg caaaattccg ccccagaacc cgcttttcgt gcgtttgtaa
     3301 taccccagcg ccagattggc cgcgacggga agcttttcga aaagggccgt caactggaag
     3361 gtccgggcga tgcctttgcg ggatatctga ctggcgctct ggcctgcgat ttcatccccc
     3421 gccaaccgga tgctgcccga tgtcggcttg cccgcgccgc tgatgaggct gaagagaacg
     3481 gatttgcccg cgccgttggg gccgatgatg gacaggattt ccccttcatg gacttcaaag
     3541 ctgacatcat tgacggcaat ggtgccgcca aaatttttta ccagattttc accttt
//
LOCUS       DAOSIM010000215         3575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_22881_length_3575_cov_7.343750, whole genome shotgun sequence.
ACCESSION   DAOSIM010000215 DAOSIM010000000
VERSION     DAOSIM010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22881_length_3575_cov_7.343750"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1684
                     /gene="dnaG"
                     /locus_tag="PLR47_08720"
     CDS             <1..1684
                     /gene="dnaG"
                     /locus_tag="PLR47_08720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOG13069.1"
                     /translation="KKAGRNYLGLCPFHQEKTPSFTVSREKQIFYCFGCGEGGNVITL
                     LMKMANKSFPEAIQYLAEKTGVLLPVRTFGRDSREKDSLHDEILRLNARAAQFFARQL
                     SSPGGRTARDYLQKRAVTEDIVRQFRMGYAPDAWRSLADDIEGGGLSLKMAEQAGLVI
                     AGKEGGFYDRFRGRLMFPIENPFGEIVAFGGRILGEGEPKYLNSPESPVYSKGKNLYG
                     LNKAKEAIRRKGFCLMVEGYFDVISLWNAGVCNVVATLGTALTRDHLELLRRYTQDVV
                     ALFDPDTAGRKALDRSLELFLGANMRARALILPDGCDPDDFVKKHGPARLDELIALAP
                     SISDYYIDTVLGNGRTFEENRDLVREALEFAGKINNEIEKNLFIKRLAEKSGVDQVLL
                     KREARRKAAPAPSGKKASAPTAMNLLELNLIRLMLEYPQRALLVEQENILDCFLENVL
                     KDLGTKILETYKLLGYVDINVLLPSDEDKPLRERLYKMSVEEPPAEDDQVERNFADNM
                     RRIREKWYKEQKRQVQHKLRQAQEGGNQDLIRELTFQKQNLMKEENKYVNKS"
     gene            1702..3315
                     /gene="rpoD"
                     /locus_tag="PLR47_08725"
                     /pseudo
     CDS             1702..3315
                     /gene="rpoD"
                     /locus_tag="PLR47_08725"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
BASE COUNT          955 a          892 c         1048 g          680 t
ORIGIN      
        1 gaaaaaggcg ggccggaatt atctggggct ttgtcccttt catcaggaaa agacgccttc
       61 gttcaccgtc agccgcgaga aacagatttt ctactgtttc ggttgcgggg aaggcggcaa
      121 tgtcattacg ctgctgatga aaatggccaa caagagtttt cccgaggcca ttcaatacct
      181 ggcggaaaaa acaggcgtcc tgttgccggt gcggaccttc ggccgggaca gtcgagaaaa
      241 ggattccctg cacgatgaga ttcttcgcct gaatgctagg gcggcgcagt tctttgcccg
      301 tcagctctcc tcgccggggg gcaggacggc ccgcgactat ctgcagaaaa gagccgtcac
      361 cgaagacatt gtccggcaat tccgcatggg atacgctccg gatgcctggc gctccctggc
      421 ggatgacatc gaaggcggcg gtttgtccct gaaaatggct gaacaggccg gtcttgtcat
      481 cgccggaaaa gaggggggtt tctatgaccg gtttcgcggc cgcctgatgt tccccattga
      541 aaatcctttc ggtgaaatcg tcgcctttgg cggacggatc ctcggagagg gggaaccaaa
      601 atatctcaac tcgccggaat cgccggttta cagcaaaggg aaaaatttat atggactcaa
      661 caaggccaaa gaggcgatcc gccgcaaggg tttttgcctc atggtggaag ggtattttga
      721 cgtaatctcc ctgtggaatg cgggggtgtg caatgtcgtg gccacgctgg gcacggcgct
      781 gacccgcgat catctggagc tgctcaggcg ctacacgcag gacgttgtcg ccctgtttga
      841 tccggacacg gccggacgca aggcgctgga ccggtcgctg gagctttttc tgggggccaa
      901 tatgcgcgcc cgtgctttaa ttttgcccga cggctgcgac ccggatgatt ttgtaaaaaa
      961 acacggaccg gcccggctgg atgagctgat cgcccttgcg ccgtccatca gcgattatta
     1021 tattgacact gttctgggaa acggcaggac atttgaagaa aaccgcgatc tggtaagaga
     1081 agcgttggaa tttgcgggga aaatcaacaa cgaaatcgaa aagaatctct ttatcaagcg
     1141 cctcgcggaa aaatcgggcg tcgatcaggt gttgttgaag cgggaggccc gccgtaaagc
     1201 ggcgcccgcc ccgtccggta aaaaagcatc tgccccgacc gcgatgaatc tcctggagct
     1261 gaatctgatc cggctcatgc tggagtatcc gcagagagcg ctcctggtgg agcaggaaaa
     1321 catactggac tgctttctgg agaacgtatt gaaagacctg ggaacgaaga tccttgagac
     1381 ttataaactg ctggggtacg tggacatcaa cgttctgctg ccctcggatg aagacaaacc
     1441 gctgcgggag cgcctttata aaatgagcgt ggaagagcct ccggccgagg acgaccaggt
     1501 ggagcgaaat tttgccgata acatgcgaag aatccgtgaa aaatggtata aggaacaaaa
     1561 gcgtcaggtt cagcacaaac tgcggcaggc gcaggaaggc gggaatcagg atctcatccg
     1621 ggaactgacc ttccagaaac agaacctgat gaaggaagaa aataaatacg tgaacaaatc
     1681 ataacattcg ggggcatcca aatggcgaaa gaaatacagt ccgatgaatt taaaaaactg
     1741 ttatccctgg gggaagaaaa gggtttttta acctatgatg acgtgaatga catgctgccg
     1801 gcagatttga attcctcgga gcaaatcgat gatattatca tgctgtttgg agaaaaaaac
     1861 atcgacatca ttgatatgga gcagggggaa aaactggtgg tgaaaaagcc ggtggaagac
     1921 gccctgccgt ccaaactccc ggaagctctc gcgctggttc ccggcgtggg aaaaaccggc
     1981 gatccggtca agatgtactt gcgggaaatg gggctggttt cgctcctgag ccgtgaaggc
     2041 gaagtcgaga tcgccaagaa aatcgaagag ggcgcccgcg aaaccatgtg ggccattttc
     2101 agcctgaccg tttccatcga cgaagtgctc agcatcggcg aaaagctgga atccggggaa
     2161 atccgcgtca aaaatgtggt ggacaacatc gaagatgaag aaggattcat ggaggaggat
     2221 gatcataaag acagagtgct ccggctgatc gcccgaatcc gtcttttcaa cgaccgcaat
     2281 aaagtcctgc gcgagaagct caaatccaag accatgaaac ccaagcaaag ggaagcattg
     2341 agcgcggagc tggcgaaaaa cacgaagaac atcatcacgc tttgccggaa aatccgcttc
     2401 agcaagaagc agatgcaccg ttttatcgca aggctgaaag tcttcgcgga cgagattgaa
     2461 cgctccgaac gcgtgatcaa tcatttcaag aaggagtcgg gcctgacgat tacgcaaatg
     2521 gaaaaggcct ggtcccgaat gaagaaatcc aaacaggacg agcggcaggc ggccaggtcc
     2581 gccaaggtct ccatcgagtg gctgcacaga agccaggcgt ccgtcacgga ggcgcaaaaa
     2641 cggctgcggc gcattgccaa ggacgtggga attccaaccg ccacgctcaa gacggtggtc
     2701 tattccatag aagacggcga agccaaggcc gaaatcgcca aacggaagct ggtcgaagcc
     2761 aacctgcggc tggtggtcag cattgccaag aaatatacca atcgcggcct gcagtttctg
     2821 gacctgattc aggaaggcaa tatcgggctc atgaaagcgg tggataaatt cgaataccag
     2881 cggggctaca agttttccac ctatgcaacc tggtggatcc gtcaggccat cacgcgcgcc
     2941 attgccgacc aggcccgcac cattcgcatt ccggtccaca tgattgaaac gatcaacaaa
     3001 ctgatccgga cgtcgcgcta ccttgttcag gaactggggc gcgaaccgac gccggaggaa
     3061 atcgccaaca aaatggaata tccgctggaa aaagtgcgca aggtgctcaa aatcgccaag
     3121 gagccgattt ccctggaaac gccgatcggc gaggaggagg acagtcatct tggggacttc
     3181 atcgaggaca aaaagatcat gtctccttcg gaggccacca tcagcatgga tctggcggac
     3241 cagacgcgca aaatcctgtc cacgctgacg ccgcgggaag aaaaggtcct gcggatgcgc
     3301 ttcggcatca gtgatcggct gggtacggcg gtcatggggg aagtgtcggt ggaggaaacc
     3361 gtgatcgccg agatggaaga gccggaagtc gtggaagaag agccgaagaa agcaaagcat
     3421 tcttcccgca agcgtccggc aaaataatat tgacaagcac cgggattggg tatatttcaa
     3481 cgcaccttga tgccggttaa atccgcgcaa atgctcaaaa aggcgggcct atagctcaat
     3541 tggtagagcc accggctcat aaccggtagg tccct
//
LOCUS       DAOSIM010000216         3525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_23273_length_3525_cov_7.982421, whole genome shotgun sequence.
ACCESSION   DAOSIM010000216 DAOSIM010000000
VERSION     DAOSIM010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23273_length_3525_cov_7.982421"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PLR47_08730"
     CDS             <1..747
                     /locus_tag="PLR47_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOG13070.1"
                     /translation="VDSLLAVKYLVFDKKVCTLPQLADALRANWKGHEVLQAMAKNRA
                     PKYGRDDDEADELALRVMQLWCAETWRHRTQSTGRRYRPGMLSWNYWAGDGYIMAASA
                     DGRTKGQFLSNAICPSNGADIKGPTSNANSVGKVLGGKAEDGRGDWLDYLNVLPNGAS
                     HTITFNPSILRDPVHKDKFKAFLKGYTQNGGTALQINMLDSGMLKDAQDHPENYRHLL
                     VRITGYNAYFTTVGRELQNEVIERVSHQQF"
     gene            760..1788
                     /locus_tag="PLR47_08735"
     CDS             760..1788
                     /locus_tag="PLR47_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOG13071.1"
                     /translation="MTSSKGKNGLVLQILRMSTEDGPGIRTSVFFKGCPLGCLWCHNP
                     ESISPKPQVQWLHTSCIGCGNCVEICPEKALEKTTDGIMINRRLCTGCGLCAEECPTT
                     AMELLGKKWSVSDLVHELVKDRVYFEQSGGGVTLTGGEAALQHEFCLPLLKELRAVGI
                     STALDTCGQAPQKTLASLLPYIDVLLYDLKEIDPEKHKGFTGAGNAKILENAVFAARF
                     KKDHPYPRTLWIRTPVIPNSTDTPENIAGIGRFIHENLEGAVDRWELCSFNNLCRDKY
                     KRLGLNWPFAEAELMKKTVMENLAGVAKSAAPKTNVCWSGSTTLEKRVDSQEPPEKQN
                     KRKPVCAG"
     gene            1812..2657
                     /locus_tag="PLR47_08740"
     CDS             1812..2657
                     /locus_tag="PLR47_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13072.1"
                     /translation="MTIKNRFSESDMKAFAPSEKVGIIATVTPEGLPHLSLLTSVMAS
                     TPTQLTIGEFSTGVSKAYLQQTRKTGFAIVTLDKKLWRGKALWTHLKKEGPEYEVYNQ
                     QPMFRYNTYFGINTVHYLELKETTEGSPLPLVAVAASAILTRLAKGAAAQSAKVKALS
                     PFGKNLFDRLDSLKFLSFIDDDGFPTVIPVIQCQACDENRLAFYPGVFAEELNRLEAG
                     KTMAIFGMTMEMEDVLVRGVFQGYARRRGIRLGTLDIDWVYNSMPPNHGQIHPPLPLK
                     AVTDF"
     gene            complement(2683..3168)
                     /locus_tag="PLR47_08745"
     CDS             complement(2683..3168)
                     /locus_tag="PLR47_08745"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOG13073.1"
                     /translation="MMVVWVLLGIMVCAGSAFAANETGRDGRFVAYDDGTVLDTTTNL
                     MWADKDNGVDIQWIAAKTFCENFSTGGYDDWRMPTKNELAGLYSGSSRDAHGYHLATS
                     LISLTGCCPWTSDTCISMLPKYEMAGYYAFNLSFITGQSVCSQQGLDSDVRVLPVRFH
                     R"
     gene            complement(3209..>3525)
                     /locus_tag="PLR47_08750"
     CDS             complement(3209..>3525)
                     /locus_tag="PLR47_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13074.1"
                     /translation="AVISKTTFDSGSPARTHSYDAGAAWVSLALQGSLKNLVVHCMQG
                     FDYDKAKEALRVPDDYAVEAMIAVGRPGNKEDLPEYLQEREFPSPRKKLDEIVMEGVF
                     QK"
BASE COUNT          816 a         1013 c          999 g          697 t
ORIGIN      
        1 gtggattcgc tcctcgccgt caagtatctg gtctttgata aaaaagtctg cacgctgccg
       61 cagttggcgg acgcgcttcg ggccaactgg aaggggcatg aagtgcttca ggccatggcc
      121 aaaaaccgcg cccccaagta cggccgggac gatgatgagg cggacgaact ggcgctgcgc
      181 gtcatgcaac tctggtgcgc agagacgtgg cggcacagga cccagtccac cgggcgccgg
      241 tatcgaccgg gaatgctgag ctggaactac tgggcgggcg acggctacat catggcggca
      301 agcgccgacg ggcgcaccaa gggtcagttt ctctccaatg ccatctgtcc gtccaacggg
      361 gcggacatca aaggaccgac ttccaacgcc aactccgtcg ggaaagtact gggcggcaaa
      421 gccgaagacg gccggggcga ctggctggac tatctgaacg tccttccgaa cggcgcgagc
      481 cacaccatta cgttcaatcc gtcgattctg cgggatccgg tgcacaagga taaattcaaa
      541 gcgtttctga aaggctacac ccagaatggc ggaaccgcgc tgcagatcaa catgctggat
      601 tccggcatgc tgaaagacgc gcaggatcat cccgaaaact accggcatct tctggtgcgg
      661 atcaccggct acaatgccta tttcacaacc gtgggaaggg aactgcaaaa tgaagtcatt
      721 gaacgggtca gtcaccagca gttttaaggc ggagaaccca tgacgtcgtc caaaggaaaa
      781 aatgggctgg ttctgcagat tctccgcatg tccacggagg acgggcccgg cattcgcacg
      841 agcgttttct tcaagggttg tcctctcggc tgcctgtggt gccacaatcc cgaaagcatc
      901 agccccaagc ctcaggttca gtggcttcac acgagctgca tcggctgcgg caactgcgtg
      961 gaaatctgcc cggaaaaagc gctggagaag acgaccgacg gcatcatgat caaccgccgg
     1021 ctctgcaccg gctgcggttt gtgcgcggaa gaatgcccca cgaccgccat ggagcttctg
     1081 ggcaaaaaat ggagcgtttc cgatctcgtc catgaactgg tcaaggaccg cgtttacttc
     1141 gaacagtccg gcggcggcgt aaccctgacc ggcggagagg cggccctgca gcacgaattc
     1201 tgtctgcccc ttttgaaaga gctgagagcc gtcgggattt ctacggccct ggacacctgc
     1261 gggcaggcgc cccaaaaaac gctggcttcc cttttgccgt acatcgatgt cctcctgtat
     1321 gacctcaagg agatcgatcc ggaaaagcac aaaggattca ccggagcggg caacgcgaaa
     1381 attctggaaa acgccgtctt cgcggcgcgc ttcaaaaaag accatccgta tcccaggacg
     1441 ctgtggatta gaacgcccgt cattccgaat tcgacggaca cgcccgaaaa catcgcgggc
     1501 atcggccggt tcattcacga aaatctggaa ggagcggttg accgctggga gctttgctcc
     1561 ttcaacaatt tatgcaggga caagtacaaa cggctgggcc tgaactggcc ttttgccgaa
     1621 gcggaactca tgaaaaaaac cgtcatggaa aatttggccg gtgtcgcgaa aagcgcggct
     1681 ccgaagacga acgtctgctg gagcggatcg acgacgctgg aaaaaagagt ggattcacaa
     1741 gaaccgccgg agaaacagaa caaacgcaaa ccggtttgcg ccggctgatt tcaagagaaa
     1801 ggaaaaattc catgaccatc aaaaaccgtt tttcggaatc ggacatgaag gcctttgcgc
     1861 cgtcggagaa agtcggcatc atcgcgaccg tgacgccgga agggctgccg cacctgtcgc
     1921 ttctgacctc ggtcatggcc tcaacgccca cgcagttgac gatcggcgag tttagcacgg
     1981 gcgtgagcaa agcctacctg cagcagaccc ggaaaaccgg ttttgccatt gtgacgctgg
     2041 acaagaaact ctggcggggg aaagcgctgt ggacacactt gaagaaagaa ggcccggaat
     2101 acgaagtcta taatcagcag ccgatgttcc gatacaatac gtatttcgga atcaacacgg
     2161 tccactatct ggagctgaag gaaaccacgg aagggtcgcc gctgcctctg gttgccgtcg
     2221 cggcgtcggc gattctgacc agactcgcga aaggcgccgc cgcgcaaagc gcgaaagtga
     2281 aggcgctttc gccgttcggg aaaaatctgt ttgaccgcct ggattccctc aaatttttat
     2341 cgttcattga cgacgacgga tttccgaccg tcatccccgt tattcaatgc caggcctgcg
     2401 acgaaaaccg tctggcgttt tatccgggcg tatttgccga agagctgaat cggctggagg
     2461 cgggaaagac aatggcgatt ttcgggatga ccatggagat ggaggatgtt ctggtccggg
     2521 gcgtctttca gggctacgcc cggcgccggg gcatcaggct gggaacgctg gatatagact
     2581 gggtctataa ttccatgccc cccaaccacg ggcagattca cccgccgctt cctctgaaag
     2641 cggtgacgga tttctgactt tttcctgctt tttcctgacc tgctaccgat ggaagcgcac
     2701 cggaaggacc cggacgtcgc tgtccaagcc ctgttgcgaa cagacgctct ggccggtgat
     2761 gaacgacagg ttgaaggcgt agtaaccggc catttcatac ttcggaagca tggaaatgca
     2821 ggtatccgaa gtccaggggc agcagcccgt caggctaatg agggatgtgg ccaggtgata
     2881 cccgtgagcg tcacgacttg agccgctgta caatcccgcc aattcatttt tcgtcggcat
     2941 ccgccagtcg tcgtatccgc ccgtgctgaa gttttcacag aatgtcttgg cggcaatcca
     3001 ttggatatcg actccgttgt ccttatccgc ccacatcaga ttggtcgtgg tatccaggac
     3061 ggtcccgtcg tcataggcga caaaccggcc atccctgccg gtttcattgg ccgcaaacgc
     3121 ggatccggcg cagaccataa tccccagcaa aacccataca accatcatga ttccacgtgt
     3181 ttttctcatc atcaccctcg ctgtacggct acttttgaaa aaccccctcc atcacgatct
     3241 catccaactt ttttcgcggc gaagggaatt cccgttcctg gagatattcg ggaaggtctt
     3301 ctttgttccc ggggcggccc acggcaatca tcgcctccac ggcgtagtca tccggcactc
     3361 gcagcgcttc tttggctttg tcatagtcga atccctgcat gcaatgcacc accagatttt
     3421 tcagcgaccc ctgcagggcc agactgaccc acgcggcccc cgcgtcatac gaatgcgttc
     3481 gcgcggggct gccggaatca aacgtggttt tggaaatcac ggcga
//
LOCUS       DAOSIM010000217         3477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_23634_length_3477_cov_7.100526, whole genome shotgun sequence.
ACCESSION   DAOSIM010000217 DAOSIM010000000
VERSION     DAOSIM010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23634_length_3477_cov_7.100526"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1181
                     /gene="pheA"
                     /locus_tag="PLR47_08755"
     CDS             102..1181
                     /gene="pheA"
                     /locus_tag="PLR47_08755"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOG13075.1"
                     /translation="MKKPLESLREEMRKKDRDIVRLLNERSEISMKIGRVKGQAGFSV
                     YDPAQEAKVIRYLRELNGGPLSDSQVTSIFREILSASRDLQRPTRVAYLGPEASFSHQ
                     ASLLHFGSSCQFFPQQDIARVFDEVEKGEVEWGVVPVENSLEGSVNVTLDRLVLTPLA
                     LQAEIDLRISQCLISQAKSLKGIQKVYSHPQALAQCRSWLRAHLPDAATGEMESTTAA
                     VQMVRGKKTAAAIGSEPAASAYGLNLLARSIEDHSSNTTRFLVIGRGETAPAGDDKTS
                     IIFAAPDVPGALHRALACFAKRKINLAKIESHPVKERLWEYFFFVDMIGHLQDRSVKN
                     CLKELKNKTVYLKTLGSYPRAKSEK"
     gene            1178..2737
                     /locus_tag="PLR47_08760"
     CDS             1178..2737
                     /locus_tag="PLR47_08760"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOG13076.1"
                     /translation="MICIPVMEPTQREALRAAAKSAVLADVIELRMDWIADGDVSELI
                     SAARRASAGVKVIVTCRRREEFLLAQENLPALPAKERTGAAKMKLLEKAVLSGADFID
                     IELAEGARAISRMRSLCHKRPNPARLIVSWHDPFKTPSLNGLKKIFDACVQAGADIVK
                     MVPYARSLSDNAAVLSLMDYAKDQGREIIAMCMGEKGQISRILAPSRGSFLGFAVLPG
                     GRPSAPGQLTLREMRAFQKLLSGRQRFFEPPQGDLHFVLLGNPVRQSLSPLMHQAAFR
                     TMGINASYSAFCVTDLAAAVAGIRAMNIQGASVTIPFKTAVMDFLDEIDADAAAVGAV
                     NTIVNDHGRLIGHNTDWSALMDALREETQLKGKKFIILGAGGTARAAAYGITKEGGRP
                     IIVNRTPEKGRKLADRFDCPFYPLMEIGKVRAFGLINTTPVGMFPDSHSSPVDSSILN
                     GFRIVADVIYNPLKTGLIRDAEAKGCRVVPGLEMFVRQGARQLNLWTGRKAPLAVMRN
                     AVRERLMRHEF"
     gene            2727..>3477
                     /locus_tag="PLR47_08765"
     CDS             2727..>3477
                     /locus_tag="PLR47_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13077.1"
                     /translation="MNSKTNLPENVSSVEVPGSKSLTQRALIAAALATGESLLRGALI
                     AEDTLRLMEGLKLLGARLEQDVSGIRVIGTGGVIANPDREIFLGNNGTALRFLTALVC
                     LGRGMYVLTGEPRLLERPVGPLVDALKDMGVKINCRNNCPPVTVSANGLKGGQIALTD
                     IESSQYVSALLLCAPCTAKGIELSLTGNIVSAPYIDLTVGVMKDFGAKITQTDRQLYR
                     VGTQPIYSGRIYDVEGDASSASYFFLAAALLR"
BASE COUNT          769 a          894 c         1086 g          728 t
ORIGIN      
        1 aatgtggtcc attttgtcct gatccggaaa atcggcatgc cgtttgtcaa cggcggcatc
       61 gaggacgaaa cgatccgcgg cgttctggag gacatgaaag catgaagaaa ccgctggaga
      121 gcctaaggga agagatgaga aaaaaggatc gggatattgt ccggctgctc aatgaacgct
      181 cggaaatatc catgaagatt ggccgggtga aggggcaggc cggtttttcg gtttacgatc
      241 cggcgcagga agccaaagtg atccggtacc tgcgtgaact gaacggcggc ccgctgtcgg
      301 acagtcaggt cacgtccatt ttccgggaga tcctctcggc ctcccgcgat ttgcagaggc
      361 caacgcgcgt cgcctacctg gggccggagg cgtctttcag ccatcaggct tcgcttttgc
      421 atttcggctc ctcctgccaa ttctttccgc agcaggacat tgcgcgggtt ttcgatgaag
      481 tggaaaaggg agaggtggaa tggggcgtcg ttcccgtaga aaattcactg gaaggttcag
      541 tcaatgtcac gctggaccgg ctggtgctga cgccgcttgc ccttcaggcg gagattgatc
      601 tgcgcatcag ccagtgcctg atttcacaag cgaaaagcct gaaaggcatc caaaaggtct
      661 attcgcatcc gcaggcgctg gcgcagtgcc ggtcttggct tcgcgcgcat ctgcccgacg
      721 ccgcaacggg tgaaatggaa agcacaacgg ccgccgttca aatggtgcgg gggaaaaaaa
      781 cggcggcagc catcggaagt gagcctgcgg cttccgccta cggtttgaac ctgctcgcgc
      841 gaagcatcga ggaccattcc tccaacacga cgcgttttct ggtgatcggc cggggcgaga
      901 cagcgcctgc cggcgatgac aaaacctcga ttattttcgc cgcgccggat gttcccgggg
      961 cgctgcaccg ggcgctggcg tgtttcgcga agcgaaagat caatctggcc aagatcgagt
     1021 cgcatccagt gaaggaaagg ctgtgggaat atttcttttt tgtggatatg atcggtcatc
     1081 tgcaggaccg gtcggtgaaa aactgcctca aggaattgaa aaacaaaacc gtatatctga
     1141 aaacactggg gtcctatccg cgggccaaga gtgaaaaatg atctgcatac ccgtcatgga
     1201 accgacgcag cgggaagctc tccgggcggc ggcaaaaagc gccgtcctgg ccgacgtgat
     1261 cgaattgcgc atggattgga tcgcggacgg agacgtgtcg gagttgatca gcgcagcaag
     1321 gcgcgcgtcc gcaggcgtca aagtcatcgt gacgtgccgc aggagagaag agtttttgct
     1381 tgcacaggaa aacctcccgg cccttcccgc gaaagaaaga accggagcgg ccaaaatgaa
     1441 actgcttgaa aaggctgttt tatccggcgc ggatttcatt gatatcgagc ttgcggaagg
     1501 ggcaagagcc atctccagaa tgcgatccct gtgccacaag cggccaaatc ccgcgcggct
     1561 gatcgtttcc tggcacgacc cgttcaaaac cccgtcgcta aacgggttga agaaaatatt
     1621 tgatgcctgt gtgcaggccg gcgcagatat cgtgaagatg gtcccgtatg cccgaagcct
     1681 gtctgataac gccgccgtac tgagcctgat ggattacgcg aaagatcagg ggcgggaaat
     1741 catcgccatg tgcatgggcg agaagggtca gatcagccgg attctggcgc cgtcgcgggg
     1801 aagcttcctg ggcttcgccg tcttgccggg cggacggccc tccgcgcccg gccagttgac
     1861 gctcagggag atgagggctt ttcagaaact tttgtccggc cgtcaacgat tttttgagcc
     1921 gccgcagggg gatctccatt ttgttcttct gggaaacccc gttcgccaga gtttgtcgcc
     1981 gttgatgcat caggcggctt tccggacaat gggcatcaac gcttcttaca gtgccttttg
     2041 cgtcaccgat ctggccgccg ctgtagccgg cattcgggca atgaacatac agggggcatc
     2101 ggttacgatt ccgttcaaaa cggcggttat ggactttctg gacgaaatcg acgcggatgc
     2161 ggcagccgtg ggagcggtca acacgatcgt gaatgatcac ggccgcctga ttggccataa
     2221 taccgactgg tctgccctga tggatgcgtt gcgtgaggaa acgcagctga agggcaaaaa
     2281 atttatcatt ctgggggcgg gcggaacagc gcgggccgcc gcgtatggaa ttacaaaaga
     2341 aggcggacgg ccgatcattg tgaatcggac gccggagaag ggcaggaaac tggccgaccg
     2401 gttcgattgt ccgttttatc ccttgatgga aatcggcaaa gtccgggctt tcggcctcat
     2461 caatacaacg ccggtgggca tgtttccgga ttctcattca tcgccggtcg attcgtccat
     2521 cctgaacggc ttccgtatcg tggcggatgt catttataac cccctgaaga ccggattgat
     2581 tcgcgatgcg gaggcgaaag ggtgcagggt tgtccccggg ctggagatgt ttgtccgcca
     2641 gggagcgcgg cagttgaact tatggacggg cagaaaagct cccctggccg tgatgagaaa
     2701 cgcagtcagg gaaaggttga tgaggcatga attctaaaac aaacctgccg gaaaatgttt
     2761 caagcgtgga ggtgcccgga tcaaagagtc tgacgcagcg ggctttgatt gccgccgcgc
     2821 tggccactgg agagtcgttg ctccggggcg ctctcattgc ggaggacacc ctgcgcctga
     2881 tggaaggtct gaagctgctg ggtgcgaggc tggaacagga tgtaagcggc ataagggtta
     2941 tcggaaccgg cggagtgatt gccaatccgg accgtgaaat atttttggga aacaacggca
     3001 cggcgctgcg gtttttaacg gcgctggttt gtctgggacg cggtatgtat gttctgaccg
     3061 gggagccgcg tctgctggaa aggccggttg gccctctggt ggatgctctg aaggacatgg
     3121 gcgtgaaaat caactgtcga aataattgtc cgccggtgac ggtatcggcc aacggactga
     3181 agggcgggca aattgcactg acggacatcg agagctccca atatgtttca gcgcttttgc
     3241 tttgcgctcc ctgcacagcc aaggggatcg aactttccct cacgggaaat atcgtttccg
     3301 cgccctacat tgatttaacc gtcggcgtca tgaaggattt cggcgcgaaa attacgcaaa
     3361 cagaccgcca actatacagg gtcggaacgc agccgattta ttcgggacgg atttacgatg
     3421 tggaaggaga tgcctccagc gcctcctatt ttttcctggc ggcagccctt ttgagaa
//
LOCUS       DAOSIM010000218         3467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_23738_length_3467_cov_7.770809, whole genome shotgun sequence.
ACCESSION   DAOSIM010000218 DAOSIM010000000
VERSION     DAOSIM010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23738_length_3467_cov_7.770809"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PLR47_08770"
     CDS             complement(<1..744)
                     /locus_tag="PLR47_08770"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG13078.1"
                     /translation="MASLWVDERDIKFQLFEMFNIGETLLGKGRYADHDADMCNMVLD
                     QAQKFAEVEIAPTYPDEVHRKPVEAVFKDGKVFAPEAYHRLYKLYCEGGWMTTADSPE
                     VGGQAFPFIIGAACANMFLAANQAFLMYPGLTHGAARLVEDFFQNPLRDIILEKMFSG
                     VWAGTMCLTEPGAGSDVGILKTTAKKNADGTYSITGTKCFISAGDHDLAENIIHPVLA
                     RIEGDPAGTKGISIFIVPKIRINEKGELGE"
     gene            complement(897..1283)
                     /locus_tag="PLR47_08775"
     CDS             complement(897..1283)
                     /locus_tag="PLR47_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOG13079.1"
                     /translation="MAIEKGQTFSGSEKVDKYRPIYYAGASGDFNPIHIDPEFGKMVG
                     LGGAILQGLCTLAFASKTVTDWAGDPGKLKRLKCRFSAPVLMEDTISVQGTVTDVQSG
                     RARIDLKVVNQNGVEVLQKVEAEVEA"
     gene            complement(1294..1731)
                     /locus_tag="PLR47_08780"
     CDS             complement(1294..1731)
                     /locus_tag="PLR47_08780"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HOG13080.1"
                     /translation="MAIDAKFIGKAYPSYTYEVGREKIKEYAKAIKNFDPHYLEDDFA
                     AKSKYGTIIAPPTFAVVYGAYLIEPVFNDRDLNLNMAMLVHGEQELEFFEVVKAGDVI
                     TTGAKIVHIQNKEKLDIIAIEVQSKNQHGRDVCRGIYTFVVRK"
     gene            complement(1793..2965)
                     /locus_tag="PLR47_08785"
     CDS             complement(1793..2965)
                     /locus_tag="PLR47_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG13081.1"
                     /translation="MNYDLTPEQLSIKDNFTKFCTKEIEPRAALLDEAPHEEVPGLIR
                     ENIKKMAGIGYLGIGHEEAYGGTNLDLISQAIAGEAVAAACASTFLSCGASSGLFGMP
                     LRLFGSEEQKKKYLPGIIKGEIIGCFGLTEPEAGSDAASIKTAAVKDGDRWILNGTKT
                     FITNATIADVALIFAYNDKAKGPGAGVTCFLVEAGTPGFKAGKPFDKMGFRGSPTAEL
                     ILEDCEVPDSAVLGTVGSGFIQAMMTLEYGRIGMATVCLGIASKCLELANKYSKERVV
                     FGKPINRFQDISFKLADMLIMSDTARLLIYQAAWAKQTGRPESAVLASVAKVWASETG
                     KQISDLAVQIHGGYGYMKEYPVERLYRDARLGEIGEGASEIQRILIAKDLIRRYAA"
     gene            complement(3016..>3467)
                     /locus_tag="PLR47_08790"
     CDS             complement(3016..>3467)
                     /locus_tag="PLR47_08790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG13082.1"
                     /translation="EYTHETLSWDRSSLLAPFVGALQFAIESCTKYSQERVQFGKPIN
                     AFQAIQHKLADIKIIQEAAKMVVYRVAHDKDSGKPLNHLHTSIAKSVVGDYGIKAASD
                     AVQIFGGYGFIHEYPIERFLRDVKLAQIGGGTSEMQKFIISRILSFM"
BASE COUNT          672 a          996 c          967 g          832 t
ORIGIN      
        1 ttcgcccagc tcgcccttct cgttgatgcg gatcttggga acgatgaaaa tggagatgcc
       61 cttggtgccg gcgggatcgc cttcgatgcg ggccaggacc gggtgaatga tgttttcggc
      121 gagatcatgg tcgccggcgg agatgaaaca tttggtgccg gtgatggaat acgtgccgtc
      181 ggcgttcttc ttggccgtag ttttcagaat gcccacgtcg gagccggcgc ccggctcggt
      241 caggcacatc gttccggccc agacaccgga gaacatcttt tcaagaataa tgtcccgcag
      301 gggattctgg aaaaagtcct ccaccaggcg cgccgcgccg tgggtcagac cggggtacat
      361 gagaaacgcc tgattggcgg ccagaaacat gttggcgcag gccgccccga tgatgaacgg
      421 aaaagcctga ccgccgactt cgggcgaatc cgccgtcgtc atccagccgc cttcgcaata
      481 aagcttgtag agacggtgat aggcttccgg tgcgaaaacc ttgccgtcct tgaagaccgc
      541 ttcgacgggt ttgcggtgga cctcatccgg ataggtgggc gcgatctcga cctcggcaaa
      601 tttctgcgcc tggtccagca ccatgttgca catatccgca tcgtgatcgg cgtaacggcc
      661 ctttcccagg agcgtttccc cgatgttgaa catttcaaaa agctggaatt tgatatccct
      721 ttcgtcaacc cacaaacttg ccatacaaac ctccttgttt tcttttattt atgaacgagt
      781 aatcgttccg gtttccccct gaagagcgtg cgggaaggtg cttcgacgaa gttcatatgg
      841 caattgagaa caattgtcaa gcgttcggga atatttattc tgcaaacggc tgcattttac
      901 gcttcgacct cggcttcaac cttctgaagc acttccacgc cgttctggtt gaccaccttc
      961 agatcaatcc tggcgcgtcc cgactgcaca tccgtgacgg ttccctggac cgaaatcgta
     1021 tcttccatca gcaccggagc gctgaaccgg catttgagcc tcttgagctt cccgggatct
     1081 cccgcccagt cggtgacggt tttggacgca aaggcaagtg tgcacaatcc ctgaagaatc
     1141 gcccctccaa gcccgaccat tttcccgaat tcgggatcga tatgaatcgg attgaaatcg
     1201 ccggatgcgc cggcgtagta aatgggcctg tatttatcga ccttctcgct tccgctgaag
     1261 gtctgtcctt tttcaatggc catggcgtgc ctcctacttt ctgacgacaa aggtgtaaat
     1321 tccacggcag acgtcccggc cgtgctggtt tttcgactga acttcaatgg cgatgatatc
     1381 cagcttttcc ttgttctgaa tgtgcactat ctttgcgccg gtggtgatga catctcccgc
     1441 cttgaccacc tcgaaaaact ccagttcctg ctctccgtgg accagcatcg ccatgttcag
     1501 gttcagatcc cggtcgttga acaccggctc gatcaggtag gcgccataga ccaccgcaaa
     1561 ggtgggcggg gcgatgatcg ttccgtactt cgatttggcg gcgaaatcgt cttccagata
     1621 atgcggatcg aaattcttga tcgctttcgc atactctttg attttttccc tgcccacctc
     1681 ataggtatag gatgggtagg ccttgccgat gaattttgcg tcgatggcca tgggtctttc
     1741 tccttgcagc gtcatgtctc cggtcggaaa gcgcgcgcct tcgtctgaac ggctaggccg
     1801 cgtacctccg gatcaggtct ttggcgatca ggatgcgctg gatttccgaa gcgccctcgc
     1861 cgatttctcc cagcctcgcg tcgcggtaga ggcgctccac ggggtattcc ttcatgtagc
     1921 cgtagccccc gtgaatctga acggccaggt cggagatctg cttgccggtc tcggaagccc
     1981 agactttggc cacggaggcc agcacggcgg actcgggacg gccggtctgc ttggcccacg
     2041 cggcctgata aatgaggagc ctcgccgtat cggacatgat cagcatatcg gcaagcttga
     2101 acgaaatgtc ctgaaagcgg ttgatcggct ttccgaagac cacgcgctcc ttggaatact
     2161 tgttggccag ctccaggcac ttggacgcaa tgcccaggca aacggtcgcc atgccgatcc
     2221 ggccgtattc cagcgtcatc atggcctgga taaagccgct gcccaccgtg cccagaacgg
     2281 cgctgtccgg cacttcgcag tcctcgagga tcagttcggc ggtcggggag ccgcgaaatc
     2341 ccattttgtc gaaaggtttg cccgctttga accccggtgt gcccgcctcc accaggaagc
     2401 aggtcacgcc ggcccccggt cccttcgcct tgtcgttata agcgaagatc agcgccacgt
     2461 cggcaatggt ggcgttggta atgaatgttt tggttccgtt gagaatccag cggtccccgt
     2521 ctttcacggc ggccgtcttg atggacgcgg cgtcggagcc ggcttccggt tcggtgaggc
     2581 caaaacagcc gatgatttcc cctttgatga tgccgggaag atattttttc ttctgctctt
     2641 cggagccgaa cagcctcagc ggcatgccga acaggccgga ggatgcgccg caggataaaa
     2701 aggtggacgc gcaggcggcc gcgaccgctt cccccgcgat ggcctggctg atcaggtcga
     2761 gattcgttcc gccgtaagct tcttcgtgtc cgatgccgag atagccgatg ccggccatct
     2821 tcttgatatt ttctctgatc agcccgggga cttcttcatg gggcgcttca tcgagcagcg
     2881 cggcgcgcgg ttcgatttcc tttgtgcaaa atttcgtaaa attgtccttt atcgacaact
     2941 gttccggcgt taaatcatag ttcatggcat attctccttc aatggatgtc tggttattct
     3001 gcttccggcc tgaggctaca taaagctcag gatgcgggag atgatgaatt tctgcatttc
     3061 cgatgtccct ccgccgatct gcgccagttt cacatccctc aaaaaccgct cgatcggata
     3121 ttcgtggatg aacccgtagc cgccgaaaat ctggactgcg tcgctggcgg ctttgatgcc
     3181 gtagtcgccg accacggatt tcgcgatgga cgtatgcaaa tgattcaaag gctttccgga
     3241 atccttgtcg tgagccacgc ggtaaacaac cattttggcg gcctcctgga tgatcttgat
     3301 gtccgccagc ttgtgctgaa tcgcctggaa cgcgttgatc ggcttgccga actgcaccct
     3361 ttcctgggag tatttggtgc aggactcgat ggcgaactgc agggcgccga cgaaaggcgc
     3421 cagaagggag ctgcggtccc aggacagggt ctcatgggtg tattcaa
//
LOCUS       DAOSIM010000219         3449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_23887_length_3449_cov_7.945197, whole genome shotgun sequence.
ACCESSION   DAOSIM010000219 DAOSIM010000000
VERSION     DAOSIM010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23887_length_3449_cov_7.945197"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PLR47_08795"
     CDS             <1..376
                     /locus_tag="PLR47_08795"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOG13083.1"
                     /translation="AVPHAPPEVAGTFNYRGTLTPVVDLTVLLTGSPSRPRMSTRIIL
                     VNYQGEDGRFHILGLLAEAVTEMIECKKENLQQPVVGVDGAAYLGDTIFDERGSLQVL
                     DMERLLTRDLLFTLFPPEVTNG"
     gene            373..1710
                     /locus_tag="PLR47_08800"
     CDS             373..1710
                     /locus_tag="PLR47_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOG13084.1"
                     /translation="MNAPAAVMEFITHSLGMEQGSAGKDAVRRAVAFAMRQEGVSDAR
                     EYEKLFLSSPAARQKLIDAVVVGETWFFRDRGPFLWLARHARQWLETHPGSTLKILSS
                     PCATGEEPYSIVMTLLAAGLPPSAFTVEGVDASARALETAGRACYPKSAFRGSIGEDV
                     ARFFETTPSGRKVAEAVASLVSFSPENLALPGSLAGRGPYDIIFCRNLLIYMTPEARL
                     RIFDRMNRLLIPGGLLFTGHTETNFWHQRGYQLLPWDRAFALAKPASPAQPAAIRTKA
                     PTKPSVRPGENFPRKTGAAAQEATSSPSNTGQGKTAGKGRGQPAKSETASENFKLSQA
                     DEQIQEARRLADTGDMKGAARLCRECTRKFGPAAEAYCLMGILSMAEQDSNGAEDYFL
                     KALYLDPGHYESLVHISLICRQKGNEKKAALYRERALRTAGNKGGSHGSEKEQ"
     gene            1707..2372
                     /locus_tag="PLR47_08805"
     CDS             1707..2372
                     /locus_tag="PLR47_08805"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOG13085.1"
                     /translation="MTTNTEKYCWKAKGIYGDGSCPELPRLIHCRRCPVFAAAGRQHL
                     DRNLPPAYLGERAAVMALPKETGRLGTLSVMVFRLCGEKFAVKTIYFQEAAEPSPVHT
                     LPLKVNRIFRGIANINGELLLCLSVADLLDLTFEAQESGPAPAYERMLVVGQEGQRFA
                     FPVEQILGVHRIAPEDLGEVPATLSRSARALTRGIFLLDGSPVGLLDEERLFPAMTRS
                     LNP"
     gene            2369..>3449
                     /locus_tag="PLR47_08810"
     CDS             2369..>3449
                     /locus_tag="PLR47_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOG13086.1"
                     /translation="MTADAAPIDPLMLDLFRVELENNTRTLEAGLVTAERDQTPERIE
                     PLMRAAHSIKGAARIVGLVKAVALAHAMEDVLSAAQSGKRPLTSAAVDRLLKGNDFFA
                     ALSRVDIQELPAALDKGADEITGMSAELRNFLSSPPESVPSPPPRQETAPVKEKPAGN
                     AAPEANAAAARTKEAEPQKPPEAAVLESACPGPQIAPPAARSRRVQDQGEGGLVRVRS
                     ENMNRLMALAGECLVQAQSVKTVYQSLLALKKEIFEIEKPLRDFVERQEANGQDEGAE
                     GLQQTLSGAAAAQQTVSREIVRFERFSRRLEHLAHRLYNEVISSRMVPFSDGLHGFPR
                     LVRDLARETGKQIRFEIAGAATPVDR"
BASE COUNT          688 a         1095 c         1063 g          603 t
ORIGIN      
        1 ggccgttcct cacgcgccgc cggaagtcgc gggaacgttc aattaccggg gaacgctcac
       61 cccggtggtg gacctgaccg tgcttctgac cggctctcca tcccgtcccc gaatgagcac
      121 gcgcattatt ctcgtcaact atcaggggga agacggccgc ttccacattc tgggtcttct
      181 ggccgaggcg gtgacggaga tgatcgaatg caaaaaggaa aaccttcagc agccggtggt
      241 cggcgtcgac ggcgcggcct atctggggga tacgatattt gacgagcggg gctcccttca
      301 ggtgctggac atggaacggc tgctgacccg cgacctgctg ttcactcttt ttccgccgga
      361 ggtcaccaac ggatgaacgc gcctgccgcc gtcatggaat tcatcacgca ttccctgggc
      421 atggagcagg gctccgccgg aaaagatgcc gtgcgccgcg ccgtcgcttt cgccatgcgt
      481 caggaaggcg tttcggatgc gcgggagtat gaaaagctgt ttttgtcctc tcccgccgca
      541 aggcagaagc tgattgacgc cgtcgtcgtc ggggaaacct ggtttttccg ggaccggggg
      601 ccttttctct ggcttgcccg ccacgcccgc caatggctgg agacgcatcc gggaagcacc
      661 ttgaagattc tgtcgtcccc ctgcgcaacc ggcgaggagc cctactccat cgtcatgacc
      721 ctgctggccg ccggcctgcc gccttcggcc ttcacggtgg aaggcgtgga cgcgagcgcg
      781 cgggccctcg agacggccgg ccgcgcctgc tacccgaaaa gcgctttccg gggaagcatc
      841 ggcgaggatg tcgcccgctt tttcgaaacg acgccttcgg gccgcaaagt cgccgaggcc
      901 gtcgcaagcc tggtctcctt ttcaccggag aacctggcgc tgcccggaag ccttgccgga
      961 cgcggtccgt acgacattat tttctgcaga aaccttctca tctacatgac ccccgaggcc
     1021 cgtcttcgga tttttgaccg gatgaacagg cttctgatcc ccggcggtct gctcttcacc
     1081 ggccatacgg aaacaaattt ctggcatcag cgcggctatc agctcctccc gtgggacagg
     1141 gcctttgccc tggccaagcc cgcttccccg gctcagcccg cggcaatcag gacaaaagcc
     1201 ccgacaaaac cgtcggtccg accgggcgaa aacttccccc gcaaaaccgg cgccgcggcg
     1261 caggaagcaa catcgtcccc ttcaaacacc ggtcagggga agactgcggg caagggacgg
     1321 gggcagccgg caaaatccga aaccgcaagt gaaaatttca aactttcgca ggcggacgag
     1381 cagattcagg aggcccggcg gctggccgac acaggcgaca tgaagggcgc cgcccgtctc
     1441 tgccgggaat gcacgcgcaa attcggaccg gccgccgaag cctattgcct gatgggaatc
     1501 ctctccatgg ccgagcagga ttcgaacggc gcggaggact attttttgaa ggccctctac
     1561 ctcgatcccg gccactacga aagcctggtc cacatcagcc tgatctgccg gcagaaggga
     1621 aacgaaaaaa aagcggcgct ttaccgggag cgcgccctgc gaacggcagg aaataaaggc
     1681 ggatcgcacg gatccgaaaa ggaacaatga ccacgaacac ggaaaaatac tgctggaagg
     1741 caaaaggaat ctacggcgac ggatcctgtc cggaactgcc ccgtctgatt cactgccgcc
     1801 gctgccccgt tttcgccgca gcgggaagac agcatctgga ccggaacctc ccgccggcat
     1861 acctcgggga gcgggccgcc gtgatggcgt tgcccaaaga gacaggcagg ctgggaacgc
     1921 tgtccgtgat ggttttccgc ctgtgcggag agaagtttgc cgtcaaaacc atctattttc
     1981 aggaagcggc ggaaccttcc ccggttcaca cactgccttt gaaggtcaac cggattttcc
     2041 ggggaatcgc caacatcaac ggagaactgc ttctgtgcct gtccgtcgcg gaccttctgg
     2101 atttgacctt cgaggcgcag gaaagcgggc ctgcgccggc ttatgagcga atgctggtgg
     2161 tcgggcagga gggacagcgc ttcgcgtttc ccgtcgagca gattctgggg gttcatcgga
     2221 ttgccccgga ggatctcggg gaagtcccgg ccacactttc acgaagcgcg agagccctca
     2281 cccggggaat tttcctgctt gacggaagcc cggtcgggct tctggatgaa gagcggctct
     2341 tccccgccat gacaaggagt ctgaacccat gaccgcggat gccgccccca ttgatccttt
     2401 gatgctcgac ctgttccgcg tggagctgga gaacaacacc cggacgctgg aagccggcct
     2461 ggtcacggcg gagcgcgatc agacgccgga gcgcatcgaa ccgctgatgc gggccgccca
     2521 ttccatcaag ggagccgccc ggatcgtggg gctcgtcaaa gccgttgcgc ttgcccacgc
     2581 catggaggac gttctgtcgg cggcacaaag cggaaaacgg ccgctgacct ccgcggcggt
     2641 ggaccggttg ctcaaaggga atgatttttt tgccgctctt tcccgcgtgg acattcagga
     2701 actgcccgcc gcgctggata agggagccga tgaaattacc ggcatgtcgg cggaactgag
     2761 aaactttctc tcctctcccc cggagagcgt tccctcgccg cccccgcggc aggaaaccgc
     2821 tcccgttaaa gaaaaaccgg ccgggaatgc agcgccagaa gccaatgccg ccgcggcgcg
     2881 caccaaagag gccgaacctc aaaagccgcc ggaggcagcc gttttggaaa gcgcctgtcc
     2941 gggaccgcaa atcgcgccgc cggccgcccg ttcccgccgc gtacaggatc agggcgaagg
     3001 gggactggta cgcgtccggt ccgaaaacat gaaccgcctg atggccctgg cgggcgaatg
     3061 cctcgttcag gcccagtccg tcaaaaccgt ctatcaaagt cttctggccc tcaaaaagga
     3121 gatctttgaa atcgaaaaac ccctgcgcga ctttgtggag cggcaggagg ccaacgggca
     3181 ggacgaagga gcggaaggcc tgcaacagac gctgtccggc gcggcggcgg cccagcagac
     3241 cgtctcgcgg gaaattgtcc ggttcgagcg tttttcacgc cgactggaac acctggcgca
     3301 tcgcctttac aacgaagtca tctcgagccg catggtgcct ttttcggacg gtctccatgg
     3361 ctttccccgt ctggtcaggg atctggcccg ggaaacgggg aaacagatcc gctttgaaat
     3421 cgccggtgcc gccacgcccg tggaccggg
//
LOCUS       DAOSIM010000220         3418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_24160_length_3418_cov_6.929825, whole genome shotgun sequence.
ACCESSION   DAOSIM010000220 DAOSIM010000000
VERSION     DAOSIM010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24160_length_3418_cov_6.929825"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="PLR47_08815"
     CDS             <1..157
                     /locus_tag="PLR47_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003084710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOG13087.1"
                     /translation="GFIEQEGGSDIFVHFSAIQSSGFKTLTEGQQVSFDVVQGKKGME
                     AENVRAL"
     gene            361..1773
                     /locus_tag="PLR47_08820"
     CDS             361..1773
                     /locus_tag="PLR47_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOG13088.1"
                     /translation="MKRKAHRLDPFFRPGSVALFGSMQEGWFFGAAVIISDLLKWNYR
                     GKIYPVHPAARTVYGIPVFPDLAHVKDIPELAVIVTSFRHAPGILEQCGAKGVKAAVI
                     VSDGFSEAGPEGRIRQEKLLGIAKSHGIQVMGPNTVGVFSSESRFTTVPYDRGYEYQK
                     QGRLSIITQTGMYSPQAMAWHEYEAGINKVIDLGNMCDVDETDCLDYLGEDPSTDVIS
                     LYMEHSRRPRMLLDVLKKVSRKKPVLCLMPGGSPGAAQAMASHTGSLAGNADLYDALL
                     RQGGALRVEEYEDLRDCATPFLRYPLPRGNRLGIITFSGAIGIQAIDAAEAFGLSIGK
                     LSPESRRKLAAQSGTLGGHPIDIGPASATAGAGIFDLYQKCFDILAADENIDCIYLNA
                     YVSNGLNPAFYEEMLGRIGSFRAKPVVSWCYGPSQQLVCEFGKLAERHGIPFFPNSKK
                     AVRSLSYMARYARRKTSVSE"
     gene            complement(1830..2315)
                     /locus_tag="PLR47_08825"
     CDS             complement(1830..2315)
                     /locus_tag="PLR47_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13089.1"
                     /translation="MKRFLKVVIAGGLVLAFAAGCASAGTMLKSEAVSAVPEKKFEKI
                     IRLDTPECLPLQPDAGFVFVEPGGKVITDFPRQIAAASLYSLDRYERLNRDRMGAFVI
                     KDRQGGVRGYYELLFEYRAFIWERGEEILLQISIPRGRRGTIADAGINAAGMGAISVG
                     R"
     gene            complement(2334..2963)
                     /locus_tag="PLR47_08830"
     CDS             complement(2334..2963)
                     /locus_tag="PLR47_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13090.1"
                     /translation="MQLNHPEATQQALGILRSGDPFQWYVIPLLVIVLYIYANEYEKG
                     NYRVIAGGLSLYMVHWFVEIVNALVQHFSGHALWTVPAGTAFEILVGVGIEISLMFSI
                     AGLVVCKLMPKDPAVKILGIPNRLFFAVVNAAFASILEIFLVRTPAFVWVYDWWGAFP
                     VFVTVYIPFFAAACYVHDWQPRGQKLFIGSLFLVNAAALVVFAGILGWI"
     gene            complement(2976..3311)
                     /locus_tag="PLR47_08835"
     CDS             complement(2976..3311)
                     /locus_tag="PLR47_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG13091.1"
                     /translation="MKNLFADIPESPEDEWMETLLRAPGFRVERIVSRGHCSPCGFWY
                     DQEENEWILLLQGSAGLRVEGQKEILVLRPGDSFHLDRRKKHRVEWTASGQNTVWLAV
                     YYTGSSNFE"
BASE COUNT          782 a         1054 c          908 g          674 t
ORIGIN      
        1 cggcttcatc gagcaagagg gcgggagtga tattttcgtg cacttcagcg cgatccagtc
       61 cagcggcttc aaaaccctca ccgagggcca gcaggtcagc tttgacgtgg ttcagggcaa
      121 aaagggcatg gaagcggaaa atgtcagagc cctctaaggg tcaggcgaaa acatccgcaa
      181 aagcgcgccg caaaacggcg cgcttttttt attccccccc gcctgcgcgc agcggttcct
      241 tcggcgggaa attgccggcc cggatcagcg cgaaatccgc cgggccaatt accatttgaa
      301 atgttttgcc ggttgcgata agggtaacgc cacacgtcac ttccctaaag gagcaccggc
      361 atgaagcgaa aggctcaccg tctggatccg tttttccgtc ccggaagcgt cgccctgttc
      421 ggcagcatgc aggaaggctg gtttttcggc gcagccgtca tcatcagcga cctgctgaaa
      481 tggaactacc ggggaaagat ctatcccgtt catcccgccg cccgaaccgt ttacggaatc
      541 ccggtctttc cggatctggc ccatgtgaag gacattcccg aactggccgt catcgtcacc
      601 tccttcaggc acgcgcccgg cattctcgaa cagtgcggcg cgaagggcgt aaaggccgcc
      661 gtcatcgttt ccgacggctt cagcgaagcg gggccggaag gcagaatccg gcaggaaaag
      721 ctgcttggaa tcgcaaaatc ccacggcatt caggtcatgg gaccaaacac cgtcggggtc
      781 ttcagctccg aaagccggtt caccacggtt ccctacgacc gcggctatga gtaccaaaaa
      841 cagggaagac tttccatcat cacccaaacc ggcatgtaca gcccgcaggc catggcgtgg
      901 catgaatacg aagccggaat caacaaagtc attgatctgg gaaatatgtg cgatgtcgat
      961 gagacggact gcctggacta tctcggcgag gacccgagca cggacgtgat ctccctgtac
     1021 atggagcact ctcgaagacc gcggatgctg ctcgacgttc taaagaaagt atcgaggaaa
     1081 aagccggtcc tgtgcctcat gccgggcggc tctcccggcg cggcgcaggc gatggcctct
     1141 cacaccggtt ctctggccgg gaacgctgac ctgtatgacg cgctccttcg gcagggcggc
     1201 gctctgaggg ttgaggaata tgaagatctt cgcgattgcg ccacgccgtt tctccgttat
     1261 cctctgcccc ggggcaatcg cctcggcatc atcaccttct ccggggccat cggcatccag
     1321 gcgatcgatg ccgccgaagc cttcggtctt tccatcggca agctttcccc ggaaagccgg
     1381 cggaagcttg ccgcccaaag cggtacgctg ggcggccacc ccatcgacat cgggccggcc
     1441 tccgccacgg caggcgcggg aatattcgac ctgtaccaaa aatgcttcga cattctcgcc
     1501 gcggatgaaa acatcgactg catttacctc aacgcctatg tttccaacgg attgaacccg
     1561 gctttttatg aagaaatgct tggccgaatc ggatccttcc gggcaaaacc ggtcgtcagc
     1621 tggtgctacg gtccgtcgca gcaactggtt tgcgaatttg gaaaactcgc cgaacgccac
     1681 ggcattccct ttttcccaaa cagcaaaaaa gccgtccggt ctctttccta catggcccga
     1741 tacgcccgcc ggaaaacatc tgtttccgaa taaccgcttc ggcgccgctt cttcctccgc
     1801 cccggacgaa cggccgcaag ccgcgcggtt tagcggccga cggaaatcgc ccccatgccc
     1861 gcggcattta ttccggcgtc cgcgatggtg ccccgcctgc ctctgggaat cgagatctgc
     1921 aatagaattt cctcgccccg ctcccagata aacgcccgat actcgaacag caattcatag
     1981 taaccgcgca caccgccctg gcggtctttg atgacaaacg cccccatgcg atcccggttc
     2041 agccgttcgt atcggtccag cgaatagagg ctggcggctg cgatctgccg cggaaaatcc
     2101 gtaatgactt taccccccgg ctccacaaag acaaatccgg catccggctg cagcggaagg
     2161 cattccggtg tgtcgagccg tatgatcttc tcaaatttct tttccggcac ggcgctcacc
     2221 gcttcgcttt tgagcatcgt gccggcagac gcgcagccgg cggcgaacgc caggacaagc
     2281 cctcccgcga tgacaacctt caaaaaccgc ttcatttttg tatcctcact ttctcagatc
     2341 cacccgagaa ttcccgcgaa cacgaccagg gcggcggcat tcacgaggaa aagtgagccg
     2401 atgaacagct tctgcccgcg cggctgccag tcatgcacgt agcaggcggc ggcaaaaaag
     2461 ggaatgtaaa ccgtcacgaa aaccggaaaa gcgccccacc agtcgtaaac ccagacaaat
     2521 gccggggttc tcaccaggaa gatttccaga atggaggcaa aagccgcatt gacgacggcg
     2581 aagaacagac gattcgggat tcccagaatc ttcacggccg gatctttcgg catcagtttg
     2641 cagaccacaa gcccggcgat cgaaaacatc aggctgattt cgatgccgac gcccaccaga
     2701 atttcaaagg cggtccccgc cggcacggtc cacagggcgt ggccggaaaa atgctgcacg
     2761 agggcgttga cgatttccac aaaccagtgg accatgtaaa gggacagtcc tccggcgatc
     2821 acgcggtaat tgcccttttc atactcattg gcatagatgt acaggacaat caccagaaga
     2881 ggaatgacat accactgaaa agggtcgccg cttcttaaga tgccgagcgc ctgctgggtt
     2941 gcctcagggt ggttcaattg catcgcatgc ctcctttatt cgaagttgga tgaaccggtg
     3001 taatagacgg ccagccatac cgtattctgc ccggaagccg tccattccac ccggtgtttc
     3061 ttgcggcgat ccaggtgaaa ggaatctccc ggacgcaaaa ccagaatttc cttttgccct
     3121 tccacgcgca agccggcgct tccctgcagg aggaggatcc attcattctc ctcctggtca
     3181 taccagaaac cgcaaggcga gcaatggccg cgggaaacaa ttctctctac gcgaaaaccg
     3241 ggcgcccgaa gaagcgtttc catccattcg tcttccggcg attcgggaat gtcggcaaaa
     3301 agatttttca cgcgttcgcc ctcccgctaa tacaatcttt aaaattgtac ttcttatacc
     3361 cgattttcat cataacgttc aaccccattg aacatttggt tcaggaaaaa gcgggaaa
//
LOCUS       DAOSIM010000221         3401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_24312_length_3401_cov_6.945906, whole genome shotgun sequence.
ACCESSION   DAOSIM010000221 DAOSIM010000000
VERSION     DAOSIM010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24312_length_3401_cov_6.945906"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /gene="nadE"
                     /locus_tag="PLR47_08840"
     CDS             <1..487
                     /gene="nadE"
                     /locus_tag="PLR47_08840"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417818.1"
                     /note="catalyzes the formation of nicotinamide adenine
                     dinucleotide (NAD) from nicotinic acid adenine
                     dinucleotide (NAAD) using either ammonia or glutamine as
                     the amide donor and ATP; ammonia-utilizing enzymes include
                     the ones from Bacillus and Escherichia coli while
                     glutamine-utilizing enzymes include the Mycobacterial one;
                     forms homodimers; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOG13092.1"
                     /translation="VAQTHQFSPEVSGILLEVLETEISPELIPGDDNGQPHQKTEEAI
                     GPYELQDFINFYITRFGYLPTKVAFLAWLAWRDKTRGAWPDVPPEKQNAYSLREIKKW
                     LHVYLMRFFKTSQYKRSCVPNGPKVGSGGSLSPRGDWRAPSDSEAAVWLEEWKKIPEK
                     E"
     gene            722..985
                     /locus_tag="PLR47_08845"
     CDS             722..985
                     /locus_tag="PLR47_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13093.1"
                     /translation="MPQITLKETVTRKLDIPLETLVKVIDSLSAADRKKLLSRIERPA
                     PSLQKFKKDKLTAIMADFAKTDLYEKEFLTEMEAGLKKSSVYR"
     gene            991..1245
                     /locus_tag="PLR47_08850"
     CDS             991..1245
                     /locus_tag="PLR47_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13094.1"
                     /translation="MPKIAELRPDLQEYINIHGLWKKWVKAKSLFENNPFHASLNTEL
                     LEPRHRAIYSFRLDKKYRALFVYLGKEVEVISITKHYRKS"
     gene            1421..1618
                     /locus_tag="PLR47_08855"
     CDS             1421..1618
                     /locus_tag="PLR47_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HOG13095.1"
                     /translation="MKPSYKDFDATELFCPQCGRAVPVRKRLLLVLSEGDKYDYSCVY
                     CGTSVGDKLVKETANTKILLR"
     gene            complement(1623..2084)
                     /locus_tag="PLR47_08860"
     CDS             complement(1623..2084)
                     /locus_tag="PLR47_08860"
                     /inference="COORDINATES: protein motif:HMM:NF008448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HOG13096.1"
                     /translation="MARMTFSNKWGRVAVAKKPQAADAKKSVEDVVREMKSAAGAPGG
                     EDYREKSLAIHGLVCARCGREFSGASRQLLTVHHKDGNHHNNPPDGSNWENLCVYCHE
                     DVHSREVLADQLGDKVSGRDVSLVYGGSSPAVSESSGLLAQKLQQALQKKK"
     gene            complement(2091..2480)
                     /locus_tag="PLR47_08865"
     CDS             complement(2091..2480)
                     /locus_tag="PLR47_08865"
                     /inference="COORDINATES: protein motif:HMM:NF017876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HOG13097.1"
                     /translation="METGLILIGVALLLLVIFSIPILWKLWRAANDVTVTLQALNERL
                     PAILKNMEDISANINDSTTAINGQVQKYSATAERFHVVIKEVAGGIETISPLILHSPL
                     FQKISALMPVMKGVRVFLDVLTGKQKG"
     gene            complement(2513..2890)
                     /locus_tag="PLR47_08870"
     CDS             complement(2513..2890)
                     /locus_tag="PLR47_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOG13098.1"
                     /translation="MAERDWNLVKAFVVGGLIGAAIGILFAPKSGKETRQDISLKADE
                     LLSKAREEYEKASEKGRAAYEAATVRARELEGLAREKAGEISEKVSELAAQSTEKLAE
                     GSGRLKKAFDAGVEAYREEKNKI"
BASE COUNT          823 a          900 c          874 g          804 t
ORIGIN      
        1 ggtggcgcag acgcatcagt tttcaccgga ggtgtccggc attctgctgg aggttttgga
       61 aacagaaatt tcgccggagc tgatcccggg cgacgacaac ggacagccgc atcagaaaac
      121 ggaagaagcc atcgggcctt atgaactgca ggatttcatc aatttttata ttacccgctt
      181 tggctacctg ccaaccaaag ttgcctttct ggcctggctg gcctggaggg ataaaacccg
      241 cggcgcatgg ccggatgttc cgcctgaaaa gcaaaacgcg tacagcctgc gggaaatcaa
      301 gaagtggctg catgtgtatt tgatgcgatt tttcaagacg tcccagtata agcgatcctg
      361 cgtgcccaac ggccccaagg tgggctcggg cggatcgctt tccccgcgcg gcgactggcg
      421 cgcgcccagc gacagcgaag cggccgtctg gcttgaggaa tggaaaaaaa tcccggaaaa
      481 ggaataattg ccccatcgtc ctcataaccg cagggcgtac cttcaggtgc gacaatccta
      541 atgaatgtag ggcgcacctt caggtgcgcc ggtcaagcgg gtctgaagac ccgccctaca
      601 aacccaacct gaattgtgca ggaaaggaaa gatcgtaatg tagggcgcac cttcaggtgc
      661 gcctgtgacg cttgaccatt caggctgatc tgctatactg tcgtcaaaag gaggttttgt
      721 catgccgcag ataacattaa aagaaaccgt cacgagaaaa ttagatattc ccctcgaaac
      781 cctggtgaag gtgattgatt ctctcagcgc ggccgacagg aagaaattgt tgtcgaggat
      841 agaacgccct gctccttctt tgcaaaaatt caaaaaagac aaattgacgg cgattatggc
      901 tgattttgcc aaaacggatc tgtatgaaaa ggaatttcta accgaaatgg aggcaggttt
      961 aaaaaaatca tccgtctaca ggtaaagcct atgccaaaga tcgccgaatt acgtccagat
     1021 ttacaagaat atatcaatat tcatggactt tggaagaaat gggtgaaagc aaaatccctt
     1081 tttgaaaaca accctttcca tgcctcctta aatacggagt tgcttgaacc aagacatcga
     1141 gccatctatt cctttcgtct cgataaaaaa tatcgcgccc ttttcgtgta tctgggtaaa
     1201 gaggttgaag tgatttccat aacaaaacat tatcggaaat cgtaacgaaa agattctcaa
     1261 aacgtagggc gaaccttcag gtgcgacaat cctaatgaat gtagggcgaa ccttcaggtg
     1321 agccggtcaa gcgggtctga taacctgacc acctaaaggt ggcacgaggt cacaagcgac
     1381 ccgccctgca ttgtaaagag aataagggag ttgttttcga atgaaaccat cctacaaaga
     1441 ttttgacgcc acggaattgt tctgtccgca gtgcgggcgg gccgttccgg tgcgcaaacg
     1501 cctgctgctg gttttatccg aaggggacaa gtacgactac agttgcgtgt actgcggaac
     1561 atccgtgggc gacaaactgg tcaaagaaac cgccaacacc aaaatccttc ttcgctaatc
     1621 acttattttt tcttctgcag ggcctgttgt aatttctggg cgagcaggcc ggaggattcc
     1681 gacaccgccg gggaagaccc gccgtagacc aggctgacgt cccggccgga cactttgtct
     1741 cccagttgat ccgccagcac ttcccggctg tggacgtcct cgtgacagta aacgcagagg
     1801 ttttcccagt tgctgccgtc cgggggattg ttgtgatgat tgccgtcctt gtggtgaacg
     1861 gtcagcaatt gccggctggc gccggaaaac tcacggccgc agcgggcgca aaccagtccg
     1921 tgaatggcga gcgacttttc acggtagtcc tcgcctccgg gagccccggc ggcgcttttc
     1981 atttcgcgaa ccacgtcttc tacggatttc ttcgcgtccg cagcctgagg ctttttggca
     2041 acggcaacgc ggccccactt attggaaaag gtcatcctgg ccatggcgca tcagcccttt
     2101 tgctttccgg tcaacacatc caggaaaacc cttacccctt tcataacggg catcagcgcc
     2161 gaaattttct gaaacaacgg cgagtgaaga atgagcgggg agatcgtctc tattccgcct
     2221 gcgacttcct ttatcaccac atgaaagcgc tcggccgtag ctgaatattt ctggacttgg
     2281 ccgttgatcg ccgtcgtcga gtcattgatg ttggcggaaa tatcttccat gttttttaaa
     2341 atggccggca gccgctcgtt taaagcctga agagtaaccg taacatcatt ggcggcgcgc
     2401 cacagtttcc acaaaatcgg aatgctgaaa atcaccagca gcagcaaggc aacgccaatc
     2461 aaaattaaac cggtttccat cgtttatctc ccccgatatt gttgcgacga agtcagattt
     2521 tgtttttttc ttcacgatag gcttccacgc ctgcgtcaaa ggcttttttt aagcggccgg
     2581 aaccctccgc tagtttctcg gtgctctgag cggccagctc actgactttt tcgctgattt
     2641 cccccgcttt ttccctggca agcccttcaa gttcccttgc gcgaacagtg gccgcttcat
     2701 aggccgctct gcctttttca gacgcttttt catactcttc tctggctttt gacagaagtt
     2761 catcggcttt caacgaaata tcctgacgtg tttcttttcc gcttttcggc gcaaacaaaa
     2821 tgccgatggc cgcgccaatc aggccgccaa ccacaaatgc tttcaccaaa ttccaatctc
     2881 tttccgccat tggttcacct ccattaaatt attttttcgc cgtttttata acacattttt
     2941 aaatgattag ccacgccgga caaaaagggg tgtcatggtg agactggcaa gcggtgagcc
     3001 tgttgaacca tgaggcgtag ggaacggtcg cggcggttcc ctgcggggtc cggggtaatg
     3061 ggcaagccag cgtcgggata aactcatggc attcgggcag cgcaaacgcc gaatgaccgc
     3121 actgaataaa aaaaccgggg aacctgtgcg ttggcaaggt tccccggttc tataatcctg
     3181 caattgatta tctgattatt ttgccggtgc aggggccgga gcttctgctg ccggagcttc
     3241 tgctgccgga gccgcgggtg ctgccggggc ttctgctgcc ggagccgcag gcgctgcggg
     3301 agcttcaacc gcaggagccg gtgcgggttc ttcctgcttg ctgcatccta ctgccgcaag
     3361 agacaaagaa gccaagaaaa gcacgctaac aaaaatggcc a
//
LOCUS       DAOSIM010000222         3376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_24557_length_3376_cov_6.690455, whole genome shotgun sequence.
ACCESSION   DAOSIM010000222 DAOSIM010000000
VERSION     DAOSIM010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24557_length_3376_cov_6.690455"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..896
                     /locus_tag="PLR47_08875"
     CDS             15..896
                     /locus_tag="PLR47_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOG13099.1"
                     /translation="MAFNPPAKMVALVGNAGVGKAKLSIFNLLLRGFMAGAYIAIGAA
                     LCTVCSTGAAPILGAGMAKLISGAVFPVGLIAIVLTGMELFTGDCMIVPVAAMMKKVT
                     WGAVMRNWVWVYIGNFVGSLAYAYVMVIGPFVTGQPDGTMAVNAFGDNAVGIAVAKIL
                     EYKAVGGAGLWSCFIKAIGCNFLVNIAILLAITADDIIGKFFGIWFPIMAFVATGFEH
                     CVANMYFLPAGKFLTDWYPAVIAKKGGLLLANGGLSWGDIWIWNLIPATIGNIFGGFL
                     FIGVTYFLMFRNDLPKD"
     gene            906..1376
                     /locus_tag="PLR47_08880"
     CDS             906..1376
                     /locus_tag="PLR47_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOG13100.1"
                     /translation="MSVFAWIVFFICAGAVALNYAATGNASFLWYAIPGLILLLVIPM
                     TLAWMSRRSFTQARQQSEHQARSCKIGKIVPAMIGNVVRISGEVRKISFKWLNRPHFH
                     IQDATGLIRVIMFTAPANKVAAGDKVEAIGIVMKYPLSKERIAISAVSVKRIEN"
     gene            1387..1527
                     /locus_tag="PLR47_08885"
     CDS             1387..1527
                     /locus_tag="PLR47_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13101.1"
                     /translation="MDSREGRCIFGFLLLGLAPVLATAGGAGAGMILFAAGLLVAITA
                     VG"
     gene            1609..2172
                     /locus_tag="PLR47_08890"
     CDS             1609..2172
                     /locus_tag="PLR47_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HOG13102.1"
                     /translation="MSDNLANPAPLGLMGFGMTTVLLNIHNAGLFEMSAVILAMGIFY
                     GGIAQIIAGCMEFKKGNTFGTTAFTSYGLFWLTLVFIIYFNGKFGIPANTMGFLACYL
                     FMWGLFTFFMWIGTWKANRGLQVVFLSLTILFWMLAARDAFGWTGTWAVLTGYEGIFC
                     GFSAIYCAMAQVINENFKRVVLPLGQK"
     gene            complement(2280..3077)
                     /locus_tag="PLR47_08895"
     CDS             complement(2280..3077)
                     /locus_tag="PLR47_08895"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOG13103.1"
                     /translation="MDVLQAIILGLVQGLGEFLPISSSAHLVLVPWLMNWPYSGLTFD
                     IALHVGTLIAVVIYFWKDWLRLLHKGFTKPKEREGKLFWYLVLATIPGALIGFLLEDV
                     AETVFRHPVLIACMLILLGVILYVVDRRGKKQIAVDHISLKTSLLIGLSQALAIIPGV
                     SRSGVTMTAALALGMTREGAARFSFLLSAPIILGAALVKAPELAANPSMVDAAFLTGM
                     AVACLSGLAAIGFLLRYVQTRTFLPFVAYRFIAGALVIGVAWFRCAS"
BASE COUNT          778 a          901 c          924 g          773 t
ORIGIN      
        1 ggaggtaacg ttttatggct ttcaatcccc ccgcaaaaat ggtcgctctc gtcggtaacg
       61 cgggagtcgg caaggctaaa ctttcaatct ttaatctgct gcttcgagga ttcatggcgg
      121 gcgcctacat cgccatcgga gcggcgctgt gtaccgtctg ctcaaccgga gcggcgccga
      181 ttctcggagc gggcatggcc aagttaatca gcggcgcggt cttccctgtc ggcctgattg
      241 ccatcgtcct caccggcatg gagctcttca ccggcgactg catgatcgtc cccgtggccg
      301 ccatgatgaa gaaggtcacc tggggcgccg tcatgagaaa ctgggtatgg gtttacatcg
      361 gaaactttgt cggctcgctg gcctacgcgt acgtcatggt gatcggaccc tttgtcacgg
      421 gacagccgga cggcaccatg gccgtgaacg cattcgggga caacgcggtc ggaatcgccg
      481 tggcgaaaat ccttgaatac aaggccgtcg gcggcgcggg cctatggtcc tgcttcatca
      541 aggccatcgg ctgtaacttc ctggtcaaca tcgccattct gctggctatt acggcggacg
      601 acatcatcgg aaaatttttc ggcatctggt tcccgatcat ggccttcgtc gcaaccggct
      661 tcgagcactg cgtggccaac atgtattttc ttccggcggg aaaattccta accgattggt
      721 acccggccgt catcgcgaag aaaggcggac tgcttctggc caacggaggg ctttcgtggg
      781 gcgatatctg gatctggaac ctgatccccg caaccatcgg aaacatcttc ggcggattcc
      841 ttttcatcgg ggttacctac ttcctcatgt tcaggaatga tctgcccaag gactagaggg
      901 aacccatgtc cgtatttgcc tggatcgtct tttttatttg cgcgggagcg gtggcgctga
      961 attacgccgc gacgggaaac gcgagctttt tgtggtatgc catccccggc ctgattctgc
     1021 ttttggtcat cccgatgacc ctggcctgga tgagccgcag aagtttcacg caggcccgcc
     1081 aacagtctga acatcaggcg cgctcctgca agattggaaa aattgtcccg gcaatgatcg
     1141 gcaatgtagt gcgaatatcg ggtgaggttc ggaaaatcag cttcaaatgg ctcaaccggc
     1201 ctcactttca cattcaagac gcgacgggat tgattcgggt catcatgttc accgcgcccg
     1261 ccaacaaagt ggcggcgggc gacaaggtgg aggccatcgg cattgtgatg aaatatccgc
     1321 tgtcgaaaga acggatcgcc atatcagccg tcagcgtcaa acgtatcgaa aattaaggag
     1381 acgattatgg actcaagaga aggaagatgc atcttcgggt ttcttttgct tggattggcc
     1441 cctgttctgg ccacggcagg cggagcgggc gcgggaatga ttctgtttgc cgcgggtttg
     1501 ctggtcgcga tcacggccgt cggttagaaa atcatttgcg gaagaaagta acggcgcaat
     1561 ccgccgttac tttcccctga cgcattttaa acattcaagg agtattttat gtctgacaat
     1621 cttgctaatc cggccccgct tggtttgatg gggtttggaa tgaccacggt tcttctaaac
     1681 atccataacg cgggcctgtt tgagatgagc gcagtgattc tggccatggg cattttctat
     1741 ggaggaatcg cgcagattat cgcgggctgc atggaattca aaaaaggcaa tacgttcggc
     1801 acgaccgctt ttacgtccta cgggctcttc tggctgaccc ttgtgttcat catttacttc
     1861 aacggaaaat tcggcatacc cgccaatacg atgggatttc tggcgtgtta tctctttatg
     1921 tggggcctgt tcacattttt catgtggatc ggcacatgga aggccaatcg gggcctgcag
     1981 gttgtctttc tgtcactgac gattctgttt tggatgctcg cggcgcgcga cgcgttcggc
     2041 tggaccggaa catgggcggt tttaaccggc tacgaaggca tcttctgcgg attttcggcc
     2101 atttactgcg cgatggcgca ggtaatcaac gaaaatttca aaagagtggt actgccgctg
     2161 gggcagaaat agattgaatt ccattgcggt cggaataaaa ttctgatcca tcgatcctga
     2221 aggaaaaaag aggctctccc gtgatcataa cgggggagcc tttttcactg gaccgggatt
     2281 tatgaagcgc acctgaacca ggcgacgcca atcaccagag cccccgcaat aaaccgatac
     2341 gccacaaacg gcagaaacgt ccgggtctga acgtagcgaa gcagaaagcc gatggcggca
     2401 aggccggaga ggcacgccac cgccatgccg gtcagaaatg ccgcatccac catggaagga
     2461 ttggcggcaa gctccggagc ttttaccagc gcggccccca gaatgatcgg cgccgagagc
     2521 agaaaggaaa agcgcgccgc gccctcgcgg gtcatgccga gcgccagcgc tgccgtcatc
     2581 gtaacgccgg agcgggaaac gccgggaata atggccagcg cctgtgacaa accaatcagc
     2641 aaactggttt tgagcgaaat atgatccacg gcaatttgtt ttttaccccg gcggtcaacc
     2701 acataaagaa taacgcccag aaggatcagc atgcaggcga tcagaacggg gtggcggaaa
     2761 accgtttcgg caacatcctc gagcaaaaag ccgatcagcg cgccgggaat cgtcgccagg
     2821 accagatacc agaagagctt tccttccctt tcttttggtt tcgtaaaccc cttatgcagc
     2881 agcctgagcc agtctttcca gaaatagatc accaccgcaa tgagggtgcc gacatgcagg
     2941 gcgatatcaa acgtcaggcc ggagtacggc cagttcatga gccagggaac cagcaccaga
     3001 tgcgcggagc tcgagatggg aagaaattcc cccagcccct gcaccaaacc gagaatgatc
     3061 gcctgcaaaa cgtccatgac cgctccttta gcctcttttc gccgtggccg ttgccttacc
     3121 tgataaaacc ggttttgtcc acccctgccg gccaggaaac agcggaaaaa aaatcctttg
     3181 cacctcttct gatcatttct ttcaatccgt tgaacatttc attgaaagaa aaacgccgaa
     3241 acgattgttt tgcgccgggt tgagtctggt gggcttacac gttaagccta ataatatctt
     3301 tatgttgcta gcctttatct tctgttttcc aattctggca caaaattttc attaagtata
     3361 aggcaaatga aacaaa
//
LOCUS       DAOSIM010000223         3371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_24612_length_3371_cov_6.634801, whole genome shotgun sequence.
ACCESSION   DAOSIM010000223 DAOSIM010000000
VERSION     DAOSIM010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24612_length_3371_cov_6.634801"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="PLR47_08900"
     CDS             complement(<1..1406)
                     /locus_tag="PLR47_08900"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOG13104.1"
                     /translation="MKTKVIFKKLLSSHQNVSFKNYLPLNSALVSDIGKAVTAIFDEL
                     GGASLLKSSGEVFIKPNAVDAKAYSHTRIEVLREIILYWKKAGAKKIYLFENSTQANY
                     TRLVYSATGYARLCRETGAKPIYLDEEKSRPLPFTGRGPVGVKDPDGYDLTSFEMPET
                     VMRLIEERDRHLYINVPKLKTHSMGVVTLGVKNQWGFPMQKCRGFDHNYNLPQKLVDV
                     LGYIRPDVTLIEGVEGTIHGHYFATALADKQVRPFRVLIAGKNVVAADIAGARVFGLN
                     VDDVPHLRIAIERGFSDGVKGPQDIELSGDIASLEKIDLIGDMPTDGHYPWDLYPEFP
                     PDVTVMKGNKLVCREGCLNNPLCVMQTMYMDNAGKGGWTLVLGRGHDPSVVDGIKGRV
                     LIAGHCAIDEVSEKLIKRLGRNKVYLSGECNNLCATVEAMFHLMKVDPLSYVPLNPLV
                     SSAAFLMARLNGSTSRVP"
     gene            1828..2916
                     /locus_tag="PLR47_08905"
     CDS             1828..2916
                     /locus_tag="PLR47_08905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOG13105.1"
                     /translation="MEKQTQTEAESTSGVFADCQLGAGFDEEFFLLNFGTRLLTAFTD
                     REILIDIALETLADFSQGKRIAIMSLDEQNKTLEIDGVFFQRQSSRPQMSFPVEKTVA
                     GKWMSQKTVGVAPLILEGEVPLPAESGRPEDSQCLCLPLIGSSFRPVGLATIEIPAGR
                     CFDFFEAQQLRVLSTALAVSLENTKLFSRVIHDSLTNLYTRRFYEIRVEEEIAKLRRN
                     SGRLSIILFDLDQFKNINDSFGHLTGDEVLRQFAALMLDHVRKGSTLISRYGGEEFIL
                     LMPDARVEEALRLAERIVSLCASHEFGDGRRKIQMTVSGGVAFTDHTEAVSQTELFER
                     ADQALYEAKHSGRNRVVVWKNISAPYRM"
     gene            2962..>3371
                     /locus_tag="PLR47_08910"
     CDS             2962..>3371
                     /locus_tag="PLR47_08910"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004396 - hexokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexokinase family protein"
                     /protein_id="HOG13106.1"
                     /translation="MTEKSGSFRVASMNQTIRTYLSNLEDLFSFSPSDAQRAAVRFHE
                     EMRRGLDGQTSSLRMLPSYVGRPTGAEQGRFLALDLGGTNLRVLAVRLDGRGNAEITA
                     VNRLAVPETVMRGPGEALFDFLAEGVAVFLEEHRL"
BASE COUNT          801 a          929 c          821 g          820 t
ORIGIN      
        1 tgcggcaccc gtgaggtcga accattcagg cgagccatca ggaatgccgc tgaagaaaca
       61 agcggattca aaggcacgta cgaaagagga tctaccttca tcagatgaaa catggcttca
      121 acggtggcgc atagattatt gcattcgccg cttaaatata ccttattccg ccccagccgt
      181 ttgatgagct tttcggatac ttcatcgatc gcgcaatgcc cggcaataag aactctgccc
      241 tttatcccat ccaccacgga tggatcgtga cctcttccca aaaccaacgt ccagccgccc
      301 ttgccggcat tgtccatata catcgtttgc attacgcaga gcgggttatt caggcaacct
      361 tcccggcaca caagtttgtt ccctttcatt actgtcacat caggcggaaa ttccggatag
      421 aggtcccagg ggtagtgccc atccgtcggc atatcgccga taaggtcaat tttttcaagg
      481 ctggcgatat cgcccgaaag ttcaatatcc tgcggcccct tcacgccgtc tgagaagccg
      541 cgttcaatcg caattctgag atggggcaca tcgtcaacat tcaatccaaa aacacgggcg
      601 ccggcaatat cggcggcaac cacattttta ccggcaatca gcacacggaa aggccgcacc
      661 tgtttatcgg caagcgccgt ggcaaaataa tgaccgtgaa tcgttccttc aacgccttca
      721 atcagcgtta catcaggcct gatgtacccg agcacatcga caagcttttg cggcaaatta
      781 tagttatggt caaaaccccg gcatttctgc atcgggaagc cccactggtt tttaacgcca
      841 agcgtcacaa ctcccatgga atgggttttc agtttcggca cattgatata caaatgacgg
      901 tcccgctctt ctatcagccg cataaccgtc tcgggcattt caaaagacgt caaatcgtag
      961 ccatcggggt ctttgactcc gacaggcccg cgaccggtaa agggcagagg ccggcttttc
     1021 tcttcatcga gatatatcgg ctttgcgccc gtttcacggc aaagccttgc atatcccgtg
     1081 gcgctataca ccaaccttgt atagtttgcc tgcgtcgagt tttcgaaaag atatattttc
     1141 ttcgcgccgg ctttcttcca atacaaaatg atttcccgaa gaacctcaat tctggtgtgg
     1201 ctgtaagctt ttgcatcaac cgcattgggt tttataaaaa cttcaccgct cgatttcagc
     1261 aacgacgccc cgcccaattc atcaaagatg gctgtgacag ccttgccgat atcactcaca
     1321 agggcgctgt tcaggggcag gtaattttta aaacttacat tctgatggct ggagagaagc
     1381 tttttaaaga taacctttgt tttcatttct tctctttttg atttgattca caaagcgtat
     1441 tgtaaagagt aaatatctgc gtgaatgttg ctgcacctgt cgcttttcat ggcagtgaat
     1501 acatatgagg gcggcttttg cttgtcaagc atatttcttg ctgcggcaag tttggttata
     1561 tgatcgtatt tggaacaaac cgattttaaa aagcaaccta tgttatcaac ttcaaaaacg
     1621 attttttatg aatatttttg tccatccggg tcttccgtac ggctacctgc tatgcgcatc
     1681 aaccggatgg accaaaaaaa gattctcttt gacagaccgg actggcctga cctatactcc
     1741 gtgccgcagg agaaaggagc gctgtttcac cttttgtcgt ttcattttcc tgaacaacac
     1801 cttatagtcg cgtacaaaag gaaccatatg gaaaaacaga ctcagaccga agcagaaagc
     1861 acctccggcg tttttgccga ctgtcaactg ggcgccggtt ttgacgagga attttttctt
     1921 ctcaatttcg gcacccggct cctcaccgcc tttaccgaca gggagattct gatcgacatt
     1981 gccctcgaaa ccctggccga tttctcccag ggaaaaagaa tcgccatcat gagcctcgac
     2041 gaacagaaca aaacattgga aatcgacggt gtcttttttc agcgccaatc ttcccgtccg
     2101 caaatgtctt ttcccgtgga aaaaacggtt gcgggaaaat ggatgtctca aaaaacggtc
     2161 ggcgtcgctc cgctcatcct ggaaggagag gttcccctcc cggcggagag cggacgcccg
     2221 gaggacagcc agtgtttgtg ccttccgctg atcgggtcct ctttccgccc ggtgggactg
     2281 gccacgattg aaatcccggc gggacgctgc ttcgactttt tcgaggcgca gcaattacga
     2341 gtcctcagca cggctctggc cgtctctctg gaaaacacaa agcttttctc ccgggtcatt
     2401 cacgacagtc tgaccaacct ttacacccgc aggttctatg aaatcagggt ggaagaagag
     2461 attgccaagc taagacgaaa cagcggacgc ctgtccatca ttctttttga tctggaccag
     2521 ttcaaaaaca tcaatgattc tttcggccac ctgacgggcg acgaagttct gcgccaattc
     2581 gccgcgctca tgctggacca cgtgcgcaaa ggctccacgc tgatcagccg ctacggagga
     2641 gaagagttca tccttctcat gcccgatgcc cgcgtggagg aagcgctccg tctggccgaa
     2701 agaatcgtct ctctgtgcgc gagccatgag tttggcgacg gccggagaaa aatccaaatg
     2761 acagtcagcg gcggtgtggc gtttaccgac catacggaag ccgtctccca gacagaactt
     2821 ttcgaaaggg cggatcaggc gctttacgaa gccaaacaca gcggacgaaa ccgtgttgtt
     2881 gtctggaaaa acataagcgc cccctaccgg atgtagtctt ttaaaattcg gaacggcgcc
     2941 gccgatgccg aaagcccgcc tgtgacggaa aaaagcggtt cttttcgcgt ggcgagtatg
     3001 aatcagacca ttcgcacata tctatcgaat ctggaggatc tcttttcctt ttctccctcc
     3061 gacgcgcaac gcgccgctgt ccgcttccat gaagaaatgc ggcgcggtct ggacggacaa
     3121 accagctctc tgagaatgct tccctcctat gtcggccgtc cgacgggcgc ggaacaggga
     3181 cgctttctgg cgctcgatct gggcggcacc aacctgcgcg tcctggcggt ccgtctggac
     3241 ggccggggca acgctgaaat cacggccgta aaccgtctgg ctgttccgga aaccgtcatg
     3301 cgcggaccgg gggaggcgct tttcgatttt ctggccgagg gcgtcgctgt ttttctggaa
     3361 gagcaccggc t
//
LOCUS       DAOSIM010000224         3350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_24800_length_3350_cov_10.177542, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000224 DAOSIM010000000
VERSION     DAOSIM010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24800_length_3350_cov_10.177542"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3350)
                     /locus_tag="PLR47_08915"
     CDS             complement(<1..>3350)
                     /locus_tag="PLR47_08915"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOG13107.1"
                     /translation="DSWVSSALKVNLERTAAEVQIPLQYAPLLEIVKDHYGLRKKTSE
                     LLTELNHPYVNWDFVLRELKSISIGNFHLYSRHADGLAGLWILLTIYFDILKSAAGED
                     VKDSALHYLFDYADTVLLHSEEFLERNLPLFPEMIESLAAMAKGDPALFRKCSSYLKR
                     IIRTAAEKNVDLATPAFDALLYKMFRATYAFWLSQPDPALWLIAERRGDAPPNETPYL
                     ELIRPLSHHRFRRLMLELEALRPREGEINGARIRDFLALPDYFQILDTYLRVASALEK
                     TEAYAGRHLVKLDFLFGIMTAPGLRDIHAGAMREINASLRLVFQEEKKENLDIFVRKI
                     FGFLKRNTSRGEFRSAGIDCILTAAREVFAQNDHPLVEAFMDELIAYGFEYPEIQGAT
                     AEWQVRVNPEHVRMIRAWLEMIALKPRWTKRLISALIIHLKTGGIFIRDTDLIQRDIS
                     RLLNTDIAPAYSLIKQLLRLFPVYFSEIGAEGELRDLTTRVDELSCRNDRLVDFFRKQ
                     SHVESNSLLVEFIEDMFRFWVSGDKKSIRKHVPDEIFDQVAGEGPYFDGMHRALLYLF
                     PLVGNQPQNFLEWDPERIAAEISRIQNVSETDRERASLMVRIYQLIYKKYHPRYHDLL
                     TDLETAGVFAAPDLSSLKRSLAEKHHYRSLTLILKCLGSLKARILSGKKTRSFENIYY
                     KRHIAAGIPSMYGTYHEEKFDALGLSLRLESLAGMLFEELIQSLNLQFITKRTIIRIH
                     TYLWTYLTALDLEGISTEGLVAKVRFITSALPIKQFSMEQYLDIFRFISRGIQDVIRD
                     YYIDAHSVNLPVILRQLQARADQTGAAAGGALPDQFIYQQSENFLRGVISSAFGLQVL
                     DNFVHTVIKTLNAELEKFKDNKRIVNLLMDYNPDLAVSPLYGRNPKMDNQILLGNKGY
                     FLKKLVSFGFQVPPGFIITTEVFRGFDAVYGYKYIFRDLARRVNREIAALERKTGRRF
                     GDPVHPLLLSVRSGATVSLPGMMRSFLNVGINAAIAENLSARKDFQWAAWDSYRRFLQ
                     TWGMFQGLSRDFFDAIMDGFKQKHGVQRKIQFPPERMRQIALAYREGILDSGIPLSDN
                     PLRQLRHAVLQVFDSWYAEQARIYRHQM"
BASE COUNT          729 a          942 c          974 g          705 t
ORIGIN      
        1 gcatctggtg gcggtagatg cgcgcctgct cggcatacca ggaatcaaaa acctgcaaaa
       61 ccgcgtggcg gagctggcga agcggattat ccgacagcgg aatgccgctg tccagaatgc
      121 cctcccggta ggcaagggcg atctgcctca tccgctcggg cggaaactga atttttctct
      181 gcacgccgtg cttctgcttg aatccgtcca tgatggcgtc gaagaaatcc cggctcagcc
      241 cctggaacat tccccaggtc tgaagaaagc ggcggtagga atcccaggcc gcccactgga
      301 aatcctttct ggcgctgagg ttttcggcaa tcgcggcgtt gatgccgacg tttaaaaaag
      361 agcgcatcat gccgggcagg gaaaccgtcg ctccgcttcg caccgacaac aaaagcggat
      421 gaacggggtc gccgaaacgc cggccggttt tccgctccag cgcggcgatt tccctgttga
      481 cccgccgggc gaggtcccgg aaaatgtact tgtacccgta gaccgcgtca aatccccgaa
      541 acacttccgt ggtgatgatg aagcccggcg gcacctgaaa cccgaaggag acgagcttct
      601 tgaggaaata gcccttgttg cccagcagaa tctggttgtc cattttcgga ttcctgccgt
      661 agaggggcga gacggcaagg tccggattgt aatccatcag aaggttgacg attcttttgt
      721 tgtccttgaa tttttccagc tccgcgttga gcgttttgat gacggtgtgg acaaaattat
      781 ccagcacctg cagtccaaag gcggaagaga tcacgccgcg cagaaaattc tccgactgct
      841 gatagatgaa ttgatccgga agggccccgc cggcggcggc gcccgtctga tccgcccgcg
      901 cctgaagctg acggagaatc accggcagat tgacgctgtg cgcgtcgatg tagtagtcgc
      961 gaatgacgtc ctgaatgccc ctggagatga accggaaaat atccaggtac tgctccatgg
     1021 aaaactgctt gatgggcagc gcgctggtga tgaaccgcac cttggccacc agtccttcgg
     1081 tggagattcc ctccagatcc agcgcggtca gataggtcca cagataggtg tgaatcctga
     1141 tgatggtgcg cttggtgatg aactgcagat tcagggactg gatcagctct tcaaacagca
     1201 tgcccgcgag gctttccagg cgcagggaaa gccccagcgc gtcgaatttc tcctcgtggt
     1261 aggttccgta catggacgga atgcccgcgg caatgtgcct tttgtagtaa atgttttcaa
     1321 acgaccgggt ttttttcccg gagagaatgc gggccttgag cgaaccgagg catttcagaa
     1381 tgagcgtcag gcttcggtaa tggtgttttt cggccagcga tcgcttcagc gacgaaaggt
     1441 ccggcgcggc aaaaacgccg gcggtctcca ggtccgtgag cagatcatga tagcggggat
     1501 gatatttctt gtagatcaac tggtaaatgc ggaccatcag cgacgcgcgc tcccggtccg
     1561 tttcggaaac attctgaatc cgggaaatct ccgcggcaat tctctccggg tcccactcca
     1621 gaaagttctg cggctgattt ccgacaaggg gaaagaggta aagcagcgca cggtgcattc
     1681 cgtcaaaata aggaccctcg ccggccacct gatcgaaaat ttcatcggga acatgcttgc
     1741 ggatggattt tttgtccccg gaaacccaga agcggaacat atcctcgata aattcaacca
     1801 gcaggctgtt gctctccacg tgggactgct tgcggaaaaa atcgaccagc cggtcgttgc
     1861 ggcaggacag ttcgtccaca cgggtggtga ggtccctcag ttctccctcc gcgccgattt
     1921 cgctgaagta cacgggaaac aggcgcaaca gctgcttgat caggctgtag gccggcgcaa
     1981 tgtccgtgtt gagcaggcgg gaaatgtcgc gctgaatcag gtccgtatcc cgaatgaaaa
     2041 tcccgccggt tttcaggtga atgatcaggg cggaaatgag ccttttcgtc cagcgcggct
     2101 tcagggcgat catttccagc caggcgcgga tcattcggac atgctcgggg ttgacccgca
     2161 cctgccattc ggccgttgct ccctgaatct ccggatattc aaaaccgtag gcaatcagtt
     2221 cgtccatgaa cgcttccacc agggggtggt cgttttgcgc aaagacttcc cgggccgccg
     2281 tgagaatgca gtcgatcccc gcgctgcgga attcgccgcg cgacgtattt cttttcagga
     2341 agccgaagat cttcctcacg aaaatatcca gattctcttt tttttcctcc tgaaaaacca
     2401 gcctgagcga cgcgttgatt tcccgcatgg cgccggcatg aatgtccctg aggcccggcg
     2461 ccgtcatgat gccgaaaagg aaatcgagct ttacgaggtg gcggcccgcg taggcttcgg
     2521 ttttttccag cgcggaggcc acccgcaggt aggtgtcgag aatctgaaaa taatccggca
     2581 gggccagaaa gtcccgaatg cgcgcgccgt ttatctcccc ttcacgcgga cgaagcgctt
     2641 cgagctccag catcagcctg cggaaccggt gatggctgag cggacggatc agctccagat
     2701 agggggtttc gttgggcggg gcatcccccc ggcgttcggc aatcagccac agcgcggggt
     2761 ccggctgact cagccagaag gcgtaggtcg cacggaacat tttgtacaaa agcgcgtcaa
     2821 acgccggcgt ggccaggtcc acgttcttct ccgccgccgt ccggatgatt cttttcaggt
     2881 aggaagagca tttcctgaac agcgccggat cgccctttgc catcgctgcc agggactcga
     2941 tcatctccgg aaacagcggc aggttcctct ccagaaattc ctcgctgtgc agaagcaccg
     3001 tgtccgcata gtcgaacaga tagtgcagcg cgctgtcctt cacgtcttcg ccggccgcgg
     3061 atttcagaat atcgaaataa atggtcagca ggatccataa accggcaagc ccgtccgcat
     3121 ggcggctgta gaggtgaaaa ttgccgatgg agatggactt gagctcccga aggacaaagt
     3181 cccagttgac atagggatga ttgagctccg tcaaaagctc gctggtcttt ttcctgaggc
     3241 cgtagtggtc tttgacaatc tcaagcagcg gcgcgtactg cagcggaatc tgaacctccg
     3301 ccgccgtgcg ctccagattg actttcagcg ccgatgaaac ccaggaatcg
//
LOCUS       DAOSIM010000225         3316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_25118_length_3316_cov_7.048451, whole genome shotgun sequence.
ACCESSION   DAOSIM010000225 DAOSIM010000000
VERSION     DAOSIM010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25118_length_3316_cov_7.048451"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PLR47_08920"
     CDS             <1..796
                     /locus_tag="PLR47_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOG13108.1"
                     /translation="AEVARECGALTVAVVTKPFQFEGKRRNAQAEEGIVQLRDVVDTL
                     IVVPNQRLLSLGGRGLSLIEAFKKADDILYQAVKGISDLIIVPGLINLDFADVKSIMS
                     SMGMALMGTGTASGENRAVEAAQRAISSPLLEDNTIQGAQGILLSITGGPDMSLYEVN
                     EASSLIQAEAHEDANIIFGTVIDENMKDEIRITVIATGFEDAKKKQVPSNVTHLGGFR
                     REDLSTPAFMRKSKSGDPANVVTMGVNDENENVDIDIPTFLRRQAD"
     gene            855..2543
                     /locus_tag="PLR47_08925"
     CDS             855..2543
                     /locus_tag="PLR47_08925"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG13109.1"
                     /translation="MNWKEKKKYDALLARERGVMKKVWGTCLTVCLAYPNLYRIGMAN
                     LGFQTVYKAINDLPSLLCERVFLPAGDGAEYAAGAVEPLSLESRKPLRDFDILAFSVS
                     FENDYPAILKMLEWSGIPLKSKDRADGHPLVIGGGIALTLNPEPLADFFDIFVLGEAE
                     EILPQFARVFAEARRLELDRKDLFNDLQKQIPGIYVPSLYAVSYFPEGKIKDIAPRTE
                     GLPKKIQISPIRNINAFCTTEVVSGADAEMADMFLVEVNRGCPHLCRFCAAGFVYAPP
                     RFRGYEEIVAAVDRGLMIKNKIGLVGTAVSDHPDLIKICRYIVGRGAQAGIGSLRLDK
                     IDESIVDLLKTGGIETVALAPEAGSQRLRDLLRKGITTDDILNAAKLLIEKEMSNLRL
                     YFMVGLPSEEDSDIDAIIDLVKKIQHTALAQTKGKRKFRRITLSINQFIPKPRTPLQW
                     CPLAEVQDVGRRIKKITHAFRSDKQIHVIADVPKWAYVQALLSSGDRRVGDILLAAHR
                     LNGNWMRAFKEVTINPDFYVYRQKDVNEFLPWDIIEAGLPKKALIKEYQKALPD"
     gene            2596..2889
                     /locus_tag="PLR47_08930"
     CDS             2596..2889
                     /locus_tag="PLR47_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13110.1"
                     /translation="MKAFSVMPTEAGIDLILISEDFRNHDYWSRIDILSEAIYDVFVP
                     IEVVGFTPEEWDNQTSPLYDYAKQGEILYAARPCQCYDAFPCFSLRKFISEPV"
     gene            complement(2958..>3316)
                     /locus_tag="PLR47_08935"
     CDS             complement(2958..>3316)
                     /locus_tag="PLR47_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13111.1"
                     /translation="DMSIEKQITVMQKIEKISKSSGGGFWATHPSWENRIKNVATTTK
                     SSSQIRNIKTTAQSPSPLMEGDQKHKEIKGIVLNDGNVVEGQLLEMSTDTVMIQTEDG
                     KVSTYSFEKEVRGFVK"
BASE COUNT          775 a          860 c          898 g          783 t
ORIGIN      
        1 cgcggaggtg gcccgggaat gcggcgcgct gaccgttgcc gtggtcacca agccgtttca
       61 gtttgaaggc aaaagaagaa acgcgcaggc ggaagaaggc atcgtgcagc tgcgcgatgt
      121 ggtggatacc ctgatcgtgg ttcccaatca gcggctgctg agcctgggcg ggcgggggct
      181 ttccctgatc gaagccttca agaaagccga tgacattctc tatcaggccg taaaaggaat
      241 atccgatctg atcatcgtcc cgggactgat caaccttgat tttgccgacg tcaagagcat
      301 catgagcagc atgggcatgg cgctgatggg gaccggcacg gccagcggcg aaaaccgcgc
      361 cgtcgaggcc gcgcagcggg cgatttcctc gccgcttttg gaggacaaca ccatccaggg
      421 cgcgcagggc atcctgctca gcatcaccgg cggtcccgac atgagcctgt acgaagtaaa
      481 cgaggcctcg tccctgattc aggccgaggc gcacgaggat gccaatatca tcttcggcac
      541 ggtcattgac gaaaacatga aagatgagat ccgcattacc gtgatcgcaa ccggcttcga
      601 ggacgccaaa aagaagcagg ttccctccaa tgtgacgcat ctgggcggct tccggcggga
      661 ggatctgtca acgccggcgt ttatgcgcaa gagcaaatcc ggcgatccgg ccaatgtggt
      721 aacgatgggc gtcaacgacg aaaacgaaaa cgtggatatc gacattccca cgttcctgcg
      781 ccggcaggcg gactaggaaa acatcgtggc tgtccgaatg cgggctggat cgcaaaggag
      841 gccatgaact gtccatgaac tggaaggaaa agaaaaaata cgatgccctg ctggctcgcg
      901 agcggggcgt gatgaaaaaa gtctggggaa cctgcctcac ggtttgcctg gcctatccca
      961 atctttaccg gatcggcatg gccaacctgg gcttccagac ggtttataaa gcaattaacg
     1021 atctcccctc ccttctttgt gagagagtgt ttctacccgc cggcgacgga gccgaatacg
     1081 ccgccggcgc ggtggaacct ctcagcctgg aaagccggaa gccgcttcgc gattttgata
     1141 ttctggcgtt ttccgtctct tttgaaaacg attaccccgc catcctgaaa atgctggaat
     1201 ggagcggcat cccgctgaaa tcgaaggacc gcgcggacgg gcacccgctg gtgatcggcg
     1261 gcggcattgc gctgacgctc aatccggagc ctctggcgga ttttttcgat atctttgttt
     1321 taggtgaagc cgaggaaatc ctgccgcagt ttgcgcgggt gtttgctgaa gcccggcggc
     1381 ttgaactcga ccggaaagac ttgttcaacg atttacaaaa acaaataccg ggaatttacg
     1441 ttccctcgct ctacgcggtg tcctattttc ccgaaggcaa gattaaggat attgcgccgc
     1501 gcacggaggg actgccaaag aaaattcaga tttcacccat cagaaatatt aatgcttttt
     1561 gcacgaccga agttgtcagc ggcgccgatg cggaaatggc ggacatgttt cttgtagaag
     1621 tcaaccgcgg ctgtcctcac ctctgccggt tttgcgcggc aggttttgtt tacgcgccgc
     1681 cgcgcttccg cggctatgaa gaaatcgtgg ccgccgtcga tcgcggtctc atgataaaaa
     1741 ataaaatcgg cctggtcggc acggcggttt ccgatcatcc cgacctgata aaaatctgcc
     1801 ggtatattgt tggtcgcgga gctcaggcgg gcatcggctc gttgcgcctg gacaagattg
     1861 atgaaagcat tgtagatctg ctcaagaccg gcggcatcga gacggtcgcc ctcgcgcccg
     1921 aggcgggaag ccagcggctg cgcgatctgc tgcgcaaggg gatcacaacg gacgatatcc
     1981 tgaacgccgc gaagcttttg attgaaaaag aaatgtccaa cctgcgtctg tactttatgg
     2041 tgggcctgcc ttcggaagaa gattccgata tcgacgccat catcgatctg gtcaaaaaaa
     2101 tccagcacac ggcgctggcc caaacaaaag gaaaacggaa attccggcgc atcacgctga
     2161 gcattaatca gtttattccc aaaccgcgaa cgccgctcca atggtgcccg ctggcggagg
     2221 ttcaggatgt ggggcgcaga atcaaaaaga tcacccacgc gtttcgctcc gacaagcaga
     2281 ttcacgtcat cgccgacgtg ccgaagtggg cctacgtgca ggcgctcttg tcttccgggg
     2341 atcgtcgtgt gggtgacatt ctgctggccg cgcatcgtct aaacggcaac tggatgcggg
     2401 cgttcaaaga ggtgaccatc aatcccgact tctacgtcta tcgtcaaaag gatgtaaatg
     2461 aattcctgcc gtgggacatc atcgaagccg gccttcccaa aaaagcgctg atcaaagaat
     2521 atcaaaaggc tctacctgat tgatcttccc tccctcgtgc gctttttaga gtatgagggg
     2581 agggattgag ggagggtgaa agccttttcc gtcatgccga cggaggcagg catcgatctg
     2641 atcctcattt cagaagactt ccggaaccat gattactggt caaggattga tatactgtcc
     2701 gaagccatct atgatgtttt tgtcccgatt gaggttgtcg gtttcacgcc tgaagagtgg
     2761 gacaaccaaa catcaccgtt atatgattat gccaagcaag gtgaaatttt atacgcagcg
     2821 cgaccatgtc aatgttatga tgcttttcct tgtttttcat tgaggaagtt tataagtgag
     2881 ccagtttaat caatgtcatt agtgattttc tgaaaagata cgaagagccc tgcccgtgta
     2941 cccccgtact ttatctgtta tttgacaaac ccacgaactt ctttttcaaa tgaataggtt
     3001 gatacctttc cgtcctctgt ctgaatcatc acggtgtccg tgctcatttc aagtagttgc
     3061 ccttcgacaa catttccatc atttaacacg atgcctttta tttctttgtg tttctgatct
     3121 ccttccatta gcggcgaagg agattgcgct gtcgttttga tgtttctaat ctgtgaagaa
     3181 gacttcgttg tcgttgctac gtttttaatc cggttttccc atgacggatg tgtcgcccaa
     3241 aaaccaccac cacttgattt agatattttt tctatttttt gcattacggt gatctgtttt
     3301 tcgatggaca tatcaa
//
LOCUS       DAOSIM010000226         3288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_25391_length_3288_cov_8.196412, whole genome shotgun sequence.
ACCESSION   DAOSIM010000226 DAOSIM010000000
VERSION     DAOSIM010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25391_length_3288_cov_8.196412"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PLR47_08940"
     CDS             complement(<1..682)
                     /locus_tag="PLR47_08940"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOG13112.1"
                     /translation="MFQKLLTGFPLLIFFLVLGAVTWLLGHILKPFFFAIFWAILLAA
                     IFDPVNEWLRRKVKNPNVSAGLALAAVILTLILPAGLILTLLVEQSLDLYQSMNQNGG
                     SWMQNLTGFLQSVGSHPLLARFQPDHAMITGKSIELLKTFAEFLVKNLSAFTQNTVVF
                     LIEFAVALYCLFYFFRDGEELFKTISRYLPVDDRHIHTFTSEFLVTAKATLKFTFVIG
                     GIQGLLGGL"
     gene            complement(698..1606)
                     /locus_tag="PLR47_08945"
     CDS             complement(698..1606)
                     /locus_tag="PLR47_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13113.1"
                     /translation="MNENEDRILFEKPNLALLRKDHAVFDMHFHTCRTDGRDTVRAIA
                     NRARALGVGIAVTDHNDIASALEMAQHRDVPSIPGIEITSREGTHILVYFDECRHLKK
                     FYTKYVQPFLGREIMSSTKLSMEEIIQSARLFPSVTIFPHPYCMAYTGICNVNFEQVR
                     LERLLDAVDGVEVINAENIHRWNLRCALLGLYLKKAITGGSDGHSLYHMGRVVTYARC
                     EPSGPAMLDAVKSGAARVVGKEINLLRKVAANTAKLKVPVAAYPERLGKNIRYSYRLI
                     HIKSILIQRQWHLRMYRRRSRLHPPV"
     gene            1731..2309
                     /locus_tag="PLR47_08950"
     CDS             1731..2309
                     /locus_tag="PLR47_08950"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG13114.1"
                     /translation="MKPQGPPGDLTLFLQALNFSAQKHRDQRRKDSAASPYINHPIEV
                     AFLLWTAGGVHDEAAILAAILHDTIEDTDTTPEEIRALFGDEVLGLVLEMSDDKSLPK
                     QERKLKQIVRSPRLSPHAKQIKLADKICNVHDIAFAPPDGWPLTRRIDYLRWAQNVID
                     GLRGANPKLEKRFDETLALARQKLASENEGKP"
     gene            complement(2310..>3288)
                     /locus_tag="PLR47_08955"
     CDS             complement(2310..>3288)
                     /locus_tag="PLR47_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13115.1"
                     /translation="FISNRFVRIEVGVDNNETKNDGLVRWAQSAGARSKPTLQENIGG
                     NFLSDSSKVTAQRQNFGPKNLLLGPNVLARLEVPAVPEPVPVPPVPAPQVPAPVPPVV
                     VPPIVVPPVVIPPAPAPEPPKPAPPAKPPKAWEYKTPFVFGNLDYPENLDFAFPRETK
                     AKGKDSFEMSRSFLVETSEYVTTNATQYAQVVELAKPVTVHKVGLALHKFGGEGWIWV
                     DIFKDQDGKPGDILHTTQMISLDDISGKPGYRWVDFKFGDKEKPVLMPGAYWIALGFS
                     GTPIMNWFYTYGKPVGPVYGTRYKSVFAQDWSGALNYEFNYRVVGMTVK"
BASE COUNT          727 a          963 c          940 g          658 t
ORIGIN      
        1 ccagcccgcc caaaagcccc tgaatgccgc cgatgacaaa agtgaatttc agcgtggctt
       61 tcgccgtgac gagaaattcc gacgtaaagg tgtggatatg tctgtcatcc accggcaggt
      121 agcgggaaat ggtcttgaac agctcttccc cgtcccggaa aaaataaaac aggcagtaga
      181 gcgccacggc aaactcgatc aggaaaacca ccgtgttctg cgtaaaggcc gaaagatttt
      241 tgaccagaaa ttcggcgaag gttttcagca gttcgatgga ttttccggta atcatcgcgt
      301 gatccggctg aaaacgcgcc agcaggggat ggctgccgac ggactgaagg aagccggtaa
      361 ggttctgcat ccacgagccg ccgttctgat tcatggattg atagaggtcc agcgattgct
      421 cgacgagcag ggtaagaatc agcccggccg gaagaatcag cgtgagaatg accgccgcga
      481 gcgccagccc cgccgaaaca ttcggatttt tcaccttccg ccgcagccat tcgttgacgg
      541 gatcgaaaat cgcggccagc agaatggccc agaaaatcgc aaagaaaaac ggcttcagaa
      601 tgtggcccag aagccaggtc accgcgccca ggaccagaaa gaagatcagc aggggaaaac
      661 cggtcagaag tttttggaac atcgaaaagc gcctccttta caccggcgga tgcagccggc
      721 ttcttcgacg gtacatgcgc aggtgccact gccgctgaat gaggatggat ttgatgtgga
      781 tcagccggta gctgtagcgg atatttttcc ccagccgctc cgggtaagcc gcgacgggaa
      841 ccttcagctt tgccgtgttc gcggccactt tccgaagcag gttgatttcc ttgccgacaa
      901 cccgggcggc gccgcttttc acggcgtcaa gcattgcggg accggatggt tcgcagcggg
      961 catacgttac aaccctgccc atgtggtaca gggagtggcc gtcgctgccg ccggtgatcg
     1021 cttttttcag gtacaggccc agcagagcgc agcgcaaatt ccagcggtga atattttcgg
     1081 cgttgatgac ctccacgccg tccaccgcgt ccagcaggcg ttccagccgc acctgctcga
     1141 aattcacgtt gcagattccc gtgtaggcca tgcagtaggg gtgaggaaaa atcgtcacgg
     1201 aaggaaaaag cctggccgat tggataatct cttccatgga gagcttcgtg gagctcatga
     1261 tctcccggcc cagaaacggc tggacgtatt tcgtgtaaaa ctttttcaaa tggcggcact
     1321 cgtcaaaata caccaggatg tgcgtgcctt cacgggaggt gatttcgatg ccgggaatgc
     1381 ttggcacgtc ccggtgctgg gccatttcca gggcgcttgc gatgtcgttg tgatcggtga
     1441 cggcaatccc cacgcccagg gcccgcgccc ggttcgcgat cgcccgcacg gtgtcgcgcc
     1501 cgtcggtgcg gcaggtgtga aaatgcatgt cgaacacggc atggtctttg cgcaaaagcg
     1561 ccaggttcgg tttttcaaaa aggattctgt cttcgttttc gttcatcaac ttctcaccgg
     1621 ttgcccgggg cgtttgttta gcacagattc ccgggaatca caaacgcgac gcggccgcat
     1681 ccttcgccgc gaatttgcgg gaatgcgccg cctgtggtat gctccagccc atgaaaccgc
     1741 aaggcccgcc gggcgacctg accctgtttt tgcaggcgct gaacttttca gcccaaaagc
     1801 atcgcgacca gcgcagaaag gattcggccg cgtcgcccta catcaaccat ccgatcgaag
     1861 tcgcctttct gctgtggacg gcgggcggcg ttcatgacga agccgcgatt cttgccgcga
     1921 ttctccacga cacgattgaa gacacggaca cgacgccgga agaaatccgc gccctcttcg
     1981 gcgacgaagt cctcggcctg gtgctggaaa tgagcgacga caaaagcctg cccaaacagg
     2041 aaagaaaact caaacagatt gtccgctccc cccggttgtc gccgcacgcc aaacagatca
     2101 agctggccga taaaatctgc aacgttcacg acatcgcctt cgccccgccg gacggctggc
     2161 cgctcacgag gagaatcgac tacctgcggt gggcccaaaa tgtcatcgac ggcctgcgcg
     2221 gcgccaaccc aaaactggaa aagcgcttcg acgaaacgct ggctttggcc cggcaaaagc
     2281 tggcctcgga aaacgaaggc aaaccgtaat tatttcactg tcatcccgac cacccggtag
     2341 ttgaattcat agttgagcgc gccgctccag tcctgggcaa agacgctctt gtagcgtgtg
     2401 ccgtaaaccg gcccgaccgg cttgccgtag gtataaaacc agttcatgat cggcgtgccc
     2461 gaaaatccca gcgcgatcca ataggcgccg ggcatcaaca ccggtttttc cttatcgccg
     2521 aatttgaaat ccacccagcg gtaaccgggc ttgccggaaa tgtcgtccag actgatcatc
     2581 tgcgtcgtgt gcagaatatc cccgggcttt ccgtcctgat ccttgaaaat atccacccag
     2641 atccatccct cgcctccgaa tttgtgaaga gccagcccca ccttatggac tgtcaccggt
     2701 tttgccagtt ccaccacctg ggcatactgc gtcgcgttgg tggtcacgta ctccgaggtt
     2761 tccaccagaa aactccttga catttcaaag gaatccttcc ctttggcttt ggtttcgcgg
     2821 ggaaacgcaa aatccagatt ttccggataa tccaggttgc cgaaaacgaa aggcgtcttg
     2881 tattcccagg ccttgggagg tttcgccggg ggcgccggtt tgggcggttc gggcgcggga
     2941 gccggcggaa tcacaaccgg cggaaccacg atcggaggca ccacgactgg cggcaccggc
     3001 gcgggaacct gaggcgcagg aaccggagga accgggacgg gttcaggtac ggcgggaacc
     3061 tccaggcgcg ccagaacatt cggccccaga agcaggttct tcggtccgaa attctgacgc
     3121 tgcgccgtca ctttgctgga atcggaaagg aagtttccgc cgatgttttc ctgcagcgtg
     3181 ggcttgcttc gcgctccggc gctctgggcc cagcggacca ggccgtcgtt cttcgtttcg
     3241 ttgttgtcca cgccgacttc gatgcgcaca aaacggttgg aaatgaaa
//
LOCUS       DAOSIM010000227         3269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_25592_length_3269_cov_10.654014, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000227 DAOSIM010000000
VERSION     DAOSIM010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25592_length_3269_cov_10.654014"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /gene="hflX"
                     /locus_tag="PLR47_08960"
     CDS             <1..904
                     /gene="hflX"
                     /locus_tag="PLR47_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOG13116.1"
                     /translation="FDHELTPAQVRSIGDFTGLKVIDRTQVILDIFARRAHSREGKIQ
                     VELAQLKYLLPRLMHKDTSLSRLAGGIGGTGPGETRLEIDRRRIRERIHRLEKDLKSV
                     AKSRGQRRGRREKSGLPVISIVGYTNAGKSTLLNTLTQSAVLAEDKLFATLDTKSARL
                     RFPRDTEAIITDTVGFIRNLPRELFSAFRATLDELQEADLLLHVIDVSGSRFEEQIAA
                     VESILEELEIHDKPAIRVFNKTDRLENKALLENLCRRFDAVPVSALDKTTLFGLLERI
                     EAALPNTNFSGRGNYDSEAGESDE"
     gene            976..2961
                     /locus_tag="PLR47_08965"
     CDS             976..2961
                     /locus_tag="PLR47_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOG13117.1"
                     /translation="MKLRYIIAVWAIVFFAASGLAAEKSITIVHTNDMHSHLQGFSPE
                     IDYQPMKLHADETLGGWSRAASVIQKFRREKNNPVLVVDAGDYTMGSFFHMLNREEAF
                     ELRLLNAMGYDVVTLGNHEFDLKPAGLAAALRTAKAKGGAPQIVFASAVFDRQKPELA
                     DLEDAFSQTGVKSCTILERGGLRIGVFGILGKDAIEVSPFAKPLKFRDPIETARDMVN
                     ILRNREKADLVVCLSHGGLRDNPKKSEDEILASEVKGIDVIVSGHTHTVLGKPIIVND
                     TIIVQAGSYGKQVGVLDIAWESGRVTLKNYALIPVNSSVAGDPKIQKMIDAFKQQLNT
                     RLLGLQNLSYDRIVAETAWDLKLVAGESPLGNLLADSIRWSVNRVSFDPNDPSSRVVV
                     AVESNGVIRDDLLRGKTGKIGFGDLFRTIPLGIGVDDTMSYPLISCYLYGHEIKRALE
                     ILTSVRPLKSNDDYYLQLSGLRFSYNPRRVYFDRVTAIAIGSEEEGYRPLDYSRSNRQ
                     LHRIAANIYNATFLQIVGRFTYSLLEIVPKDKNGKPLAKLSDALVDADPVLPGIQELK
                     EWEGVLRYVQSFPDTNGNGIPDLPAKYSGTLGRIVEAPSCNPVHLFSKPAASTIIVLA
                     AAGVVLLLIVAAVMVRKGRRLKAEGSKSAGSYKTEGF"
     gene            2930..>3269
                     /locus_tag="PLR47_08970"
     CDS             2930..>3269
                     /locus_tag="PLR47_08970"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOG13118.1"
                     /translation="MRDHTKLKAFELADNLVDLVYRATESFPKEELFGLTSQIRRSAI
                     SIPSNIVEGCARHTQADYIRFLYIAFGSLRELHYQLNLSKRLGFLTNEALLSIEPKVT
                     ETEKVLNGLIK"
BASE COUNT          809 a          875 c          904 g          681 t
ORIGIN      
        1 ctttgaccat gaactgacgc ccgcgcaggt gcgctccatc ggcgatttta ccggactcaa
       61 agtaatcgac cgcacgcagg tgattctgga tatctttgcc cgccgcgctc acagccgcga
      121 aggaaaaatt caggtggagc tggcgcagct caagtatctg ctcccgcgtc tcatgcacaa
      181 agacacgtcc ttatcaagac tggcgggcgg catcgggggg acgggcccgg gggaaaccag
      241 gctggaaatc gaccgccgcc gaatccgcga gcgcattcac cggctggaaa aagatttgaa
      301 atccgtggcc aagtcgcgcg gccagcgaag gggcagaagg gaaaaatcgg gccttccggt
      361 gatttccatt gtcggctata ccaacgcggg caagtccacg ctgctcaaca cgctgacgca
      421 aagcgccgtc ctcgcggaag acaagctgtt tgccaccctc gacacgaaaa gcgcgaggct
      481 gcggtttccg cgggatacgg aagccatcat tacggacacg gtcggtttta tccggaacct
      541 gccccgggaa ctgttcagcg ctttccgggc gacgcttgac gaacttcagg aagcggacct
      601 gctgctgcac gtcattgacg tgagcggcag ccggtttgaa gaacagattg cggcggtcga
      661 aagcatcctg gaagagctgg aaatccacga caagccggcc attcgcgttt tcaacaaaac
      721 ggaccgcctt gaaaacaagg ctctgctgga gaatctgtgc aggcggtttg acgccgtacc
      781 cgtatccgct ctggacaaaa ccaccctctt cggacttctg gaaaggattg aagcggcgct
      841 gccgaataca aacttttcag gacgggggaa ttatgatagt gaagcggggg aaagtgacga
      901 gtgaaaagac aggtcagagg taatgggtaa tgggttgaag gactcaccat gacagaaaag
      961 gaaaaaggga aaataatgaa attaagatat atcattgctg tctgggccat cgtgtttttc
     1021 gccgccagtg gtttggctgc cgaaaaatcg attaccattg tacacaccaa tgacatgcac
     1081 tcccacctgc agggattttc gccggagatc gactatcaac cgatgaagct ccatgccgac
     1141 gagacccttg gcggctggtc gcgcgcggca agcgttatac aaaaattcag aagagaaaaa
     1201 aacaatccgg tcctagtcgt ggatgcgggc gattacacga tggggtcttt ttttcacatg
     1261 ctgaaccgtg aagaggcttt cgaactgcgc ctgctcaacg ccatgggcta tgacgttgtt
     1321 acgctgggca accatgaatt cgacctcaag ccggcagggc tggccgccgc cctcagaacg
     1381 gccaaagcca aaggcggagc gccgcagatc gttttcgcca gcgccgtatt tgaccggcaa
     1441 aagcctgagc tggcggatct ggaagacgcg ttttcccaaa ccggcgtgaa aagctgcacg
     1501 atcctggagc ggggcggact gagaatcggc gtctttggaa ttctgggaaa ggacgccatc
     1561 gaggtctctc ctttcgccaa acccctgaaa ttccgagacc ccatcgagac cgcccgggac
     1621 atggtgaata tccttcggaa tcgggaaaaa gcggacctgg tggtctgcct ctcgcacggc
     1681 ggacttcgcg acaaccccaa aaaatccgaa gatgaaatcc tggcctccga agtcaaaggc
     1741 atcgacgtta tcgtcagcgg tcacacgcac accgtgctcg gcaagccgat catcgtcaat
     1801 gacactatca tcgttcaggc cgggagttac ggaaagcagg tcggcgttct ggatatcgcc
     1861 tgggaatcag gccgggtgac actgaaaaac tacgccttga tccccgtcaa cagttccgtt
     1921 gccggagatc cgaagattca gaagatgatc gacgccttca aacagcagct caacacgcgc
     1981 ctgctcggtt tgcaaaatct gtcctacgac cggatcgtcg ccgaaaccgc gtgggatctg
     2041 aaactcgttg ccggtgaatc gcccctgggc aatctgctgg ccgattccat ccgctggtcc
     2101 gtcaaccggg tctcgttcga tccaaacgat ccgtcaagcc gggtggtggt tgccgtcgaa
     2161 tccaacggcg tcatacgtga cgacctgctt cggggaaaaa ccggaaagat cggcttcggc
     2221 gatctgttcc gcacgattcc gctgggcatc ggcgtggacg ataccatgag ctatcccctg
     2281 atttcctgtt acctgtacgg gcatgaaatc aaaagagcgc tggaaattct gaccagcgtg
     2341 cggccgctca aaagcaatga tgactattat ctgcagctct ccgggctccg cttttcctat
     2401 aatccgcgcc gggtttactt tgaccgcgtc accgccatag caatcggcag cgaggaagag
     2461 ggataccggc ccctagatta cagccggtcc aaccggcaat tgcaccggat cgcagccaat
     2521 atttacaatg cgactttttt gcagattgtg ggccggttta cctattcgct tttggaaatc
     2581 gttccgaaag acaaaaacgg aaagcctctg gcaaaactct ccgacgcgct cgtggacgcc
     2641 gacccggtcc tgcccggcat tcaggagctg aaagaatggg agggcgtcct tcggtacgtt
     2701 cagtcctttc cggacaccaa cggcaacggc attccggatc ttccggctaa atattcggga
     2761 acactcggcc gcatcgtgga ggcgcccagc tgcaacccgg tccatctgtt ctccaaacct
     2821 gccgcctcga ccatcatcgt gctggcggcg gcaggcgtcg ttttgttgtt gattgttgca
     2881 gcggttatgg taagaaaagg ccgaaggctg aaggctgaag gaagcaagag tgcgggatca
     2941 tacaaaactg aaggcttttg agctggcgga caatcttgtt gatttagttt atagagcaac
     3001 agaaagcttt ccaaaagaag agttattcgg acttacttcg caaattcgta ggtcggcgat
     3061 ttcaatacct tcaaatattg ttgaagggtg cgcaagacac actcaagccg attatataag
     3121 atttctttat attgcattcg gttcgttaag agaactgcat tatcagttga atttatccaa
     3181 gcggctgggc ttcctgacaa atgaagcatt attgagtatt gaaccaaaag ttactgaaac
     3241 tgaaaaagtg ctgaacggct tgatcaaat
//
LOCUS       DAOSIM010000228         3246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_25840_length_3246_cov_8.568160, whole genome shotgun sequence.
ACCESSION   DAOSIM010000228 DAOSIM010000000
VERSION     DAOSIM010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25840_length_3246_cov_8.568160"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1593)
                     /locus_tag="PLR47_08975"
     CDS             complement(<1..1593)
                     /locus_tag="PLR47_08975"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOG13119.1"
                     /translation="MLYSEMHLPTGREVPSDAEVVSNQLMIRAGMIRKLTSGIYSYLP
                     LGYRVIRKVEQIVREEMNKAGAQEVHLPMVQPAELWQESGRWNYYGKELLRFRDRNDR
                     DYCLGPTHEEVITDLVRHDIKTYRQLPKNLYQIQTKFRDEVRPRFGVMRCREFGMKDA
                     YSFDADEAGAEKSYEKMFAAYNNIFRRCGLKFRPVEADSGSIGGSFSHEFMVLAESGE
                     DAIAFCEKCDYAANLEKAEIARPEKTEPGDGDRPALESVETPNVRTIDEVSAFLNVSP
                     QQIVKTLIFNADGRACAVLIRGDHEVNEIKVKNFLGAAELELADDEMIFKATGAPRGF
                     AGPVGVKVRVIADYSILGLADMVTGANRENHHLKNVNLGRDFAVEAFADLRVVQPGDA
                     CPRCAGEIKFARGIEVGHVFKLGTKYSKAMKAVYLDRDGREKTMIMGCYGIGIGRTAA
                     ACIEQNFDQNGIIWPMPLAPYAVIVTPVNINETEVMKAAREIYDELLAAGVEVILDDR
                     DERAGVKFKDADLIGIPLRIVVG"
     gene            complement(1603..2673)
                     /gene="ispG"
                     /locus_tag="PLR47_08980"
                     /gene_synonym="gcpE"
     CDS             complement(1603..2673)
                     /gene="ispG"
                     /locus_tag="PLR47_08980"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOG13120.1"
                     /translation="MEASFAARRKTRALQVGCVAIGGDATVAVQSMTSTDTRDVAATV
                     RQIRALERAGCEIIRVAIPDAEAVRALPDIKKRIAIPLIADIHFQARLAVAAIQKGVD
                     GIRINPGNMAGDKIAEIVRAAKDHQTVIRIGVNAGSLEKDLLARYGVSARALCESALR
                     GAARLEDLGFERIKLSIKSSDVPMMVEACRLVAARTDYPLHLGVTEAGTRVDAAVKSA
                     LGIGMLLADGIGDTIRVSVTGNPVLELPIAWSILRALKIRMVGAEIISCPTCGRCEID
                     LAGLAVRVEKALAGRKKYIKVALMGCVVNGPGEAAEADIGIAGGRGFGMLFKKGVACK
                     KVKEKDFVPVLLEEIRSLDKEG"
     gene            complement(2691..>3246)
                     /locus_tag="PLR47_08985"
     CDS             complement(2691..>3246)
                     /locus_tag="PLR47_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOG13121.1"
                     /translation="GVGHAVGYAMESGIPYRRPLVKYTPGYGRSYTPPTQEIRDLVAT
                     MKLVAVRDVINGCRMVICDDSIVRGTQLKNLTVKKLWDNGAKEIHIRPACPPLMYPCI
                     YASSTRTTSELASRKAIRALEGKDLEDISEYLDTTSAKHAKMIDWIRRDLNVTSLQYM
                     TIEDMIAAIGLPADQLCLHCWLGK"
BASE COUNT          562 a         1026 c          944 g          714 t
ORIGIN      
        1 ccccacgaca atccgcaggg gaatgccgat cagatcggcg tccttgaatt tcacgcctgc
       61 gcgctcatcc cggtcgtcga ggatcacttc cacacccgcc gccagaagtt catcgtaaat
      121 ctcccgggcc gccttcatca cctccgtctc gttgatgttg acgggcgtga cgatcactgc
      181 gtagggcgcg agcggcatcg gccagatgat gccgttttgg tcaaagtttt gttcgatgca
      241 ggcggccgcc gtccggccga tgccgatgcc gtagcagccc atgatcatgg ttttttccct
      301 gccgtcgcga tcgaggtaaa cggctttcat ggccttcgag tatttcgtgc ccagcttgaa
      361 aacgtgtccg acttctatgc cgcgggcgaa tttgatttcg cccgcgcagc gcgggcaggc
      421 gtcgcccggc tgaaccacgc gcagatccgc gaaagcttcc acggcaaagt ccctgccgag
      481 gttcacgttt ttcaggtgat gattttcacg gttcgccccg gtcaccatgt cggccagccc
      541 gaggatcgaa taatcggcga tcacacgtac tttgacgccg acgggacccg cgaagccgcg
      601 cggcgcgccg gtggccttga aaatcatttc gtcgtcggcc agttccagtt cggccgcgcc
      661 cagaaaattt ttcaccttga tttcgtttac ctcatgatcg ccgcgaatga ggaccgcgca
      721 ggccctgccg tccgcgttga agatgagcgt tttgacgatc tgttgcggag aaacgttcag
      781 aaaagcgctg acctcgtcaa tggtgcgcac attcggcgtc tccacgcttt ccagggccgg
      841 ccggtccccg tcgccgggtt cggttttttc gggtctcgcg atttccgcct tttccagatt
      901 ggccgcatag tcgcattttt cgcagaacgc gatggcatcc tcgcccgatt ctgccagcac
      961 catgaactcg tgcgaaaaac tgccgccgat gctgccggaa tccgcttcca ccgggcggaa
     1021 tttgaggccg caccgccgga agatgttgtt gtaggcggca aacattttct cgtagctttt
     1081 ttccgcgccc gcctcgtccg cgtcaaagct gtaagcgtcc ttcatgccga attcccggca
     1141 gcgcatcacg ccgaagcgcg gacgcacttc atcgcggaac ttggtctgaa tctgatacag
     1201 gttcttcgga agctggcggt aggtcttgat gtcgtggcgg accaggtccg tgatgacttc
     1261 ctcgtgggtg ggccccagac agtagtcgcg gtcgttgcgg tcgcggaacc gaaggagctc
     1321 cttgccgtag tagttccagc ggcccgattc ctgccagagc tccgccggct gcaccatggg
     1381 caggtgcact tcctgggcgc cggctttgtt catttcctcc cggacaatct gctccacttt
     1441 tcgaatgacc cggtacccga gcggcagata ggaatagatg ccgctggtga gcttgcggat
     1501 cattcccgcc cgaatcatca gttgattgct gaccacctcc gcgtcggacg gcacttcccg
     1561 tccggtgggc aggtgcattt ccgaataaag cattcctcct ccttaccctt ccttgtccag
     1621 ggatcgaatt tcttcgagca gcaccgggac aaaatctttt tccttcactt ttttgcaggc
     1681 aacgcccttt ttgaacagca tgccgaagcc gcggcccccg gcaatgccga tgtccgcttc
     1741 cgccgcttcg ccggggccgt tgaccacgca gcccatcagc gccaccttga tatatttttt
     1801 tctgccggcc agggcttttt ccacccgtac cgccaggccg gccagatcga tctcgcaacg
     1861 cccgcacgtc gggcaggaga tgatttccgc cccgaccatt cgaatcttca acgcccgcag
     1921 aatgctccag gcaatcggca gttccaaaac ggggttgccc gtgacggaca cccggatggt
     1981 gtcgccgatg ccgtcggcca gcagcatgcc gatgcccagc gcggatttga ccgccgcgtc
     2041 cacccgcgtt cccgcctccg tcactcccag gtgcaggggg taatccgtcc tggcggcgac
     2101 caggcggcag gcttccacca tcatcggcac gtcggaggat ttgattgaca gtttgatcct
     2161 ttcaaagccc aggtcttcca gccgggctgc gccgcgcagg gcgctttcgc aaagggcccg
     2221 ggctgaaacg ccgtatcggg ccagcaggtc cttctcgagc gaaccggcgt tgacgccgat
     2281 ccggatgacc gtctgatgat ctttggccgc ccggacgatt tcggcgattt tgtccccggc
     2341 catgttgccc ggattgatcc gaatgccgtc cacccctttt tgaatggcgg cgacggccag
     2401 gcgtgcctgg aaatgaatat cggcgatcag cgggatggca attcttttct tgatgtccgg
     2461 cagcgcccgg accgcttccg cgtcgggaat ggccacccgg ataatttcac agcccgcacg
     2521 ctccagggcg cggatctgcc gtacggtcgc cgcaacatcg cgggtgtccg tactggtcat
     2581 ggactggacg gcaaccgtcg cgtcccctcc tatggcgacg caacccacct gaagggctct
     2641 ggtttttcgt cttgctgcaa aagaggcttc cataggactg atttctgaag tcattttccc
     2701 agccagcagt gcaggcagag ctggtccgcg ggaaggccga tggccgcgat catgtcttca
     2761 atcgtcatgt actgaagcga cgtgacgttc aggtcgcgcc ggatccagtc gatcattttc
     2821 gcgtgctttg ccgacgtcgt atcgagatac tccgaaatat cctcgaggtc cttcccctcc
     2881 agggcgcgga tcgccttgcg gctggccagc tcggaggtgg tccgggtgga ggaggcgtaa
     2941 atgcagggat acatcagcgg cggacaggcc ggacggatat ggatttcctt cgctccgttg
     3001 tcccacagtt ttttgaccgt cagattcttg agctgcgtgc cgcggacgat ggaatcgtcg
     3061 cagatgacca tccggcagcc gttgatgacg tcgcgcacgg caaccagctt catggtggcc
     3121 accaggtcgc gaatttcctg ggtcgggggc gtgtagcttc ttccataacc cggggtgtac
     3181 ttgaccagcg gacgccggta gggaatgccg gattccatgg cgtagccgac ggcatgcccg
     3241 acgccg
//
LOCUS       DAOSIM010000229         3238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_25924_length_3238_cov_6.994345, whole genome shotgun sequence.
ACCESSION   DAOSIM010000229 DAOSIM010000000
VERSION     DAOSIM010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25924_length_3238_cov_6.994345"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PLR47_08990"
     CDS             <1..538
                     /locus_tag="PLR47_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOG13122.1"
                     /translation="EIALRTQQILAEETGVVNTIDPLAGSYFVESLTNEMEAKAWEYI
                     EKIDAMGGMIAAIERGYPQMEIADAAYAFQRQVEAGEKIMVGVNKYATEARHPVPLVE
                     IDEAVGEEQVRRLKDLRRRRNGRAVSQSLADIRAACKSGENVMPHCIAAVKNLATQQE
                     ICDVYREVYGEYQDPGLF"
     gene            608..1012
                     /locus_tag="PLR47_08995"
     CDS             608..1012
                     /locus_tag="PLR47_08995"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOG13123.1"
                     /translation="MPERKIRVMVAKPGLDGHDRGARILARCFRDAGFEVIYTGCHQT
                     PEQVAAAAIQEDVDLVGLSCLSGAHRVLFPRVVELLREKNASDIAVIGGGIIPEQDFQ
                     MLYDAGIQAIFTPGASLDSIVEWIRTHVRTGN"
     gene            993..2009
                     /gene="meaB"
                     /locus_tag="PLR47_09000"
     CDS             993..2009
                     /gene="meaB"
                     /locus_tag="PLR47_09000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOG13124.1"
                     /translation="MSGPETDGRGKHHDMTETTQKICAGDVRSASRLIRDLEDKRPEA
                     RRRLRQLFFHTGRSFIIGITGSPGAGKSTLTDALISELRKQQKTVGVLAVDPSSPFSG
                     GALLGDRVRMLRHAEDTGVFIRSLATRGALGGLARAAGDAIHVMEAMGKDVILVETVG
                     IGQQELDIIHHAHSIIVVLVPGMGDEIQTMKAGVMEIADVFVINKADRPGAKQLRTEL
                     ASLLNASPHGPQDWIPPIVSIGNLYEPENFAKKTAELMDAVAKHHAHLITSGQLGERT
                     ERKAVMEITDALRACILEPVLERLIADGELTKIAKRIQRRETDPFTEAETIARRFLKT
                     GETP"
     gene            2006..2587
                     /locus_tag="PLR47_09005"
     CDS             2006..2587
                     /locus_tag="PLR47_09005"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOG13125.1"
                     /translation="MKKKPGKAAPKSKPARADAAANRGLVIVITGDGKGKTTSAFGQA
                     LRAVGQGYKVYMMQFMKGRNYGEFKAARRCLPSLTIRRSGLDSFVMRGSPAPVDIELA
                     RKGFESARKAIASGRYDMVILDEINVAADFKLIELADLIQLIQSRPPGLDLILTGRYA
                     AREVIRLADTVSEIKDIKHHYAAGIKGRAGIEY"
     gene            2606..>3238
                     /locus_tag="PLR47_09010"
     CDS             2606..>3238
                     /locus_tag="PLR47_09010"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOG13126.1"
                     /translation="MFAWFASPEAWVSLATLTALEIVLGIDNIIFISILVGRLPAQKR
                     NFARNLGLLLAMLTRLALLFSIVWVANLTQPWFTLFGLDISGRDIILIGGGLFLLAKA
                     THEIHSSLEGVEEEKPKLKTVSLAPVLIQIALLDVVFSLDSVITAVGLARHISIMAIA
                     IIVAVGIMLVAAKPIAEFVDAHPTIKILALSFLILVGVTLMVEGFDVHVPK"
BASE COUNT          695 a          980 c          990 g          573 t
ORIGIN      
        1 ggaaatcgcg ctgcgcaccc agcagattct ggccgaagaa acgggcgtgg tcaatacgat
       61 cgatcccctg gccggctcct acttcgtcga atccctgacc aacgagatgg aggcgaaagc
      121 ctgggagtac atcgaaaaaa tcgacgccat gggcggcatg atcgccgcca tcgaaagagg
      181 ctatccgcag atggaaatcg cggacgcggc ctacgcgttc cagcggcagg tcgaagccgg
      241 ggaaaaaatc atggtgggcg tcaacaaata cgccaccgaa gcgcggcacc cggtgccgct
      301 ggtggaaatt gacgaagcag tgggcgaaga gcaggtccgg cggctgaaag atctgcgcag
      361 acgccgcaac ggccgagccg tgagccagtc gcttgccgac attcgcgccg cctgcaaaag
      421 cggtgaaaac gtcatgccgc actgcatcgc ggcggtaaaa aacctggcca ctcagcagga
      481 aatctgcgac gtgtaccgcg aggtttacgg agagtaccaa gacccgggtt tattctaaaa
      541 cggcagagaa cggccgcgac agtccaagag tcgcaagtcc gtttcctgcc gcaatcatgg
      601 agattttatg ccggaaagaa aaattcgagt gatggtggcc aagcccggcc tggacggcca
      661 cgaccggggc gcgcgcatcc tggcgcgctg ttttcgcgac gccggttttg aagtcattta
      721 caccggctgt caccaaaccc cggagcaggt ggcggccgcg gcgattcagg aggacgtgga
      781 cctcgtgggg ctaagctgcc tgtcgggcgc gcatcgcgtt ctgtttccgc gcgtcgtgga
      841 gctgcttcgg gaaaagaacg cctccgatat tgccgtgatc gggggcggca tcattcccga
      901 gcaggatttt caaatgctct acgacgcggg catccaggcg atctttacgc ccggcgcttc
      961 gctggattcc attgtagaat ggattcgcac ccatgtccgg accggaaact gacgggcgcg
     1021 gcaaacatca cgacatgacc gaaaccaccc agaaaatatg cgcgggcgac gtccgctcgg
     1081 cctcgcggct gatccgggac ctggaagaca aaaggcccga agcgcgccgg cggctccggc
     1141 agcttttttt tcacacgggc cgatccttca tcatcggcat caccggctcg cccggcgcgg
     1201 gcaaaagcac gctcaccgat gcgctgatca gcgagcttcg caaacagcaa aaaaccgtgg
     1261 gcgttctggc cgttgatccg agcagcccct tttccggagg cgcgctcttg ggcgaccgcg
     1321 tccgcatgct gcgccatgcg gaagacacgg gcgtgttcat ccggtcgctg gccacgcgcg
     1381 gcgcactggg cgggctggcg cgtgcggcgg gcgacgcgat tcacgtcatg gaagccatgg
     1441 gcaaggacgt catcctggtg gagacggtcg gcatcggcca gcaggagctc gacatcattc
     1501 accatgcgca cagcatcatc gtggtgctgg ttcccggcat gggcgatgaa atccagacca
     1561 tgaaagcggg cgtcatggaa atcgccgacg tatttgtcat caacaaggcg gaccgtccgg
     1621 gcgcaaagca gttgagaacc gagctggcct cgctgctcaa cgcctcgccg cacgggcctc
     1681 aggactggat tccgcccatc gtcagcatcg gcaatctgta tgaaccggaa aacttcgcca
     1741 aaaaaacggc ggagctgatg gacgcggtcg cgaagcacca cgcgcacctc atcaccagcg
     1801 ggcagctcgg cgagcggacg gagcgcaagg ccgtcatgga aatcaccgac gccctgcgcg
     1861 cctgcattct ggagccggtg ctcgaacgac tgatcgcgga cggcgagctc acaaaaatcg
     1921 ccaaaagaat tcagcgccgg gaaacggacc ccttcaccga ggccgaaacc atcgcccgac
     1981 gtttcctcaa aacgggagaa accccatgaa aaagaaaccc gggaaagccg ccccaaaaag
     2041 caaaccggcg cgtgcggacg cagcggcaaa ccgaggcctg gtcatcgtga tcaccggcga
     2101 cggcaagggc aagaccacct ccgcgttcgg acaggcgctg cgcgccgtcg gccaggggta
     2161 caaagtgtac atgatgcagt tcatgaaagg ccgcaactac ggcgagttca aagccgcccg
     2221 ccgatgcctg ccttccctga cgatccgccg ctcaggcctc gacagcttcg tcatgcgcgg
     2281 cagtccggcc ccggtggata ttgaactggc ccgaaagggc tttgaatctg ccagaaaagc
     2341 gattgcgtcc ggccggtacg atatggtcat tctcgacgaa atcaacgtgg cggcggactt
     2401 taagctcatt gagcttgcgg atctgattca attgattcaa agcaggccgc cggggctcga
     2461 tttgatcctc accggccggt acgcggcccg ggaagtcatt cggctggccg acacggtcag
     2521 tgaaatcaag gacatcaaac accattacgc cgccggcatc aaggggcggg cgggaattga
     2581 atactgattt tttccggaga aagatatgtt tgcatggttt gccagtccgg aggcctgggt
     2641 ctccctggcc accctgacgg ccctggagat cgtcctgggc atcgacaaca tcatttttat
     2701 ttccattctg gtggggaggc tccccgccca aaaaagaaac ttcgcgcgca atctgggatt
     2761 gttgctggcg atgctcaccc ggctggcgct gcttttttcg atcgtctggg tggccaacct
     2821 gacccagccc tggttcaccc ttttcgggct ggacatttcc ggtcgggata tcattttaat
     2881 cggcggcggg ctgtttctgc tggccaaggc cacgcatgaa atccacagca gcctggaagg
     2941 ggtcgaggag gaaaaaccga agctcaaaac cgtgtccctg gcgccggtcc tgattcagat
     3001 cgcgcttctg gacgtcgttt tctcgctgga ttccgtcatc acagccgtcg gcctggcccg
     3061 gcacatctcg atcatggcga tcgccatcat cgtcgccgtg ggcatcatgc tggtcgccgc
     3121 caagccgatt gccgaattcg tggacgcgca cccgaccatc aagattctgg cgctttcctt
     3181 cctgatcctg gtgggcgtca cgctgatggt cgaaggcttt gacgtgcatg tgccgaaa
//
LOCUS       DAOSIM010000230         3223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_26088_length_3223_cov_8.031250, whole genome shotgun sequence.
ACCESSION   DAOSIM010000230 DAOSIM010000000
VERSION     DAOSIM010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26088_length_3223_cov_8.031250"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2451)
                     /locus_tag="PLR47_09015"
     CDS             complement(<1..2451)
                     /locus_tag="PLR47_09015"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HOG13127.1"
                     /translation="MKSSVYQWGRLLLWIAADETQAEEQWPFAGGPFRPLKRPSVFFK
                     NLPSDYLLALDAEKSVSCGPAELRRLLALARDTRAGMIYSDYYEKNGSRFAGRPLNDY
                     QAGSLRDHFHFGPFFILSGAAVSSAVRKYGALPADPDSAFYDLRLRISLEHELIRVPE
                     FLYTVSAKEKMPSKKTGRKSEAHFSYVARENLIRQKQLEKIATRHLRRLGAWLPPRPA
                     ADRQKEEILWKASIVIPVFNRRKTIADALQSALAQQTDFPFNILVVDNHSTDGTTKLL
                     KTFAAQYPHVHHLVPSRHDLGIGGCWNEAIYSPLCGRYAVQLDSDDLYASPHTLQIMI
                     NELRRGPCAMAVGSYTLVNEHLKKIPPGLIDHREWTARNGHNNLLRVDGIGAPRAFDT
                     AILRRIGFPNVSYGEDYAAALRLSREYRIGRVYESVYLCRRWKDNTDAGLSVEKQNRN
                     DFYKDRLRTLEIKARQQINEKEKSTENINDKNQKECCHSRAGGNPKFLNTGFRVALRL
                     PGMTNEAVAAPPQTADRQTGSSVSLAPNQIFAEFPEGGSQTLHALCRALVESQKKSWP
                     AFAAACRDLASVQTRELSLGDYSVTLQYNPARTVSSGAAVDAESIRKRPCFLCAENRP
                     EEQSGILYRGDYQILCNPAPIFEHHLTVAALTHLPQDIRLSLDAFLDIAHDAARDYAV
                     FYNGPACGASAPDHLHFQMIPARALPFLNELKEGSFPIAPSAILHRPAKNCDRRVLLF
                     KSDHAGLLKEHMTRFLDAARKQLATQEEPLLNVFCTYGKHGWTITVFLRRKHRPDAYF
                     APGKSGIFVSPGAIDMAGV"
     gene            complement(2468..3001)
                     /locus_tag="PLR47_09020"
     CDS             complement(2468..3001)
                     /locus_tag="PLR47_09020"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOG13128.1"
                     /translation="MKKPGVLSAIRIGFLCLSCFCLIGCGYGFAPQGEHIDKRIVNIY
                     VAPFVNKTAEAEAENILRAAFIDQILQSSRFKSALTLEQADAVINGTVISITTGVLSY
                     RNNILAAEERMTITLEASLREKDSGKTIWSSQNVAGTSDYKLQDSVNPKPARQQALAK
                     LAKDTAENTFNLMMSDF"
     gene            complement(3012..>3223)
                     /locus_tag="PLR47_09025"
     CDS             complement(3012..>3223)
                     /locus_tag="PLR47_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13129.1"
                     /translation="ACATEEEMTVVIQINGKLRSRMTVPVDCGAEKIKADALADEKIA
                     SLIGGNKVLKVIYVPKKLVNLVVAS"
BASE COUNT          640 a          866 c          973 g          744 t
ORIGIN      
        1 gacgccggcc atgtcaatgg cgccgggcga gacgaagatg ccgcttttcc ccggtgcaaa
       61 ataggcgtcg ggccggtgtt tccgcctcaa aaacacggtg atcgtccaac cgtgctttcc
      121 gtaagtgcaa aaaacattca gcagaggctc ttcctgcgtc gcaagctgtt tccgggcggc
      181 atccaggaat cgcgtcatgt gctctttcag gagtccggca tggtccgact tgaagagcag
      241 gacgcggcga tcgcagtttt ttgccggacg atgcaggatg gcggatggcg caatcgggaa
      301 agatccttct ttcagttcat tcaaaaatgg gagcgcacgg gccggaatca tctgaaagtg
      361 caggtggtcc ggcgcggaag ccccgcaggc cgggccgttg tagaatacgg catagtcccg
      421 cgctgcgtca tgggcgatat ccagaaatgc gtccagtgat aaccggatat cctgcggcag
      481 gtgcgtgagc gccgccacgg tgaggtggtg ttcgaagatg ggcgccgggt tgcagaggat
      541 ctgataatcg ccgcggtaca gaatgccgga ctgctcttcc ggacggtttt cggcgcagag
      601 aaaacagggc cgtttccgga tcgattccgc gtccacggcc gcgccgctgc tgacggtgcg
      661 ggccgggttg tactgcaggg tgacgctgta gtcgcccaag gagagttccc gggtttgcac
      721 cgacgccaga tcacggcagg ccgccgcgaa ggcaggccag cttttctttt gcgactcaac
      781 gagcgcgcgg cacaaggcgt gcagggtttg gcttccgcct tcaggaaatt cagcaaagat
      841 ttgattcggt gcaagtgaaa cagaggaacc agtctgtcgg tctgctgttt gaggaggcgc
      901 cgcaactgct tcatttgtca ttccgggcaa gcgaagcgcg acccggaatc cagtatttaa
      961 aaattttgga ttcccgcctg cgcgggaatg acaacactct ttttgatttt tatcgtttat
     1021 attctcagta gatttttctt tttcgtttat ctgctgcctg gctttgattt ccagcgttcg
     1081 cagccggtcc ttgtaaaaat cattgcggtt ttgcttttcc acggacaggc ctgcgtcggt
     1141 gttgtccttc cagcggcggc agagatagac gctttcataa acccggccga tgcggtactc
     1201 gcgggagagc ctcagcgccg cggcgtagtc ttccccgtaa ctcacattgg gaaagccgat
     1261 ccggcgaaga atggccgtgt cgaaagcccg cggggcgccg atgccgtcca ctcgcaaaag
     1321 attgttgtgg ccgtttcgcg cggtccactc ccgatgatcg atgagacccg gcggaatctt
     1381 tttcaaatgt tcgtttacca gtgtatagga gcccacagcc atcgcgcagg gaccgcggcg
     1441 cagttcgttg atcataattt gcagcgtgtg cggcgacgcg taaagatcgt cggaatccag
     1501 ttgaacggcg tagcggccgc acaggggcga gtaaatcgcc tcgttccagc atccgccgat
     1561 gcccagatca tgccgggacg gaacaagatg atgaacatga gggtattgag cggcaaaggt
     1621 tttcagaagc ttcgtcgtgc cgtccgtgga gtggttgtcc accaccagaa tattgaaggg
     1681 aaaatccgtt tgttgcgcaa gcgcgctttg aagggcgtcg gctatggttt tccggcgatt
     1741 gaatacggga atgacaatgc tggccttcca gagaatctct tccttctgcc ggtccgcggc
     1801 gggccttgga ggcagccagg cgcccaggcg ccgcagatgg cgggtggcta ttttttccag
     1861 ttgtttttgc cggatcaaat tctcccgggc gacgtaggaa aaatgcgctt cggatttcct
     1921 gccggttttt ttggaaggca ttttttcttt tgcggatacc gtgtagagga attcgggcac
     1981 gcggatcagt tcatgctcca gcgaaattct caggcgcaaa tcgtagaagg cgctgtccgg
     2041 atcggccggc agcgcgccgt attttcgaac cgctgaagag acggcggcgc ccgataaaat
     2101 gaaaaagggg ccgaaatgaa aatgatcgcg cagggatccg gcctggtagt cgttcaacgg
     2161 ccgcccggcg aaacggctgc cgtttttctc gtagtagtcc gaataaatca tgccggcccg
     2221 cgtgtcgcgg gcaagggcca gaagtcttcg aagctccgca gggccgcagg acacgctttt
     2281 ttccgcatcc agcgccagca gataatccga cggaaggttt ttgaaaaata cggaaggccg
     2341 tttcagcggc cggaagggac cgcctgcgaa aggccattgc tcttccgcct gcgtttcatc
     2401 cgcggcgatc cagagcaaaa gccgtcccca ttgatacact gaacttttca tgatttcccg
     2461 gacgccgtca gaagtcggac atcatcagat taaaggtgtt ttccgccgtg tccttcgcca
     2521 gcttggccag ggcctgttgc cgggccggtt tcggattgac gctgtcctgc agtttgtagt
     2581 ccgacgtgcc tgcgacattt tgcgaagacc agatggtttt gccgctgtcc ttttcgcgaa
     2641 ggctggcctc cagggtaatc gtcattctct cttccgcggc cagaatgttg ttgcggtaag
     2701 aaagaacacc cgtggtgatg ctgatgacgg tgccgttgat gacggcgtcc gcctgttcaa
     2761 gggtaagcgc gcttttgaaa cggctgctct gaaggatctg gtcgatgaag gccgcccgca
     2821 gaatgttttc cgcttcggcc tccgcggttt tgttgacgaa aggcgcgacg taaatgttga
     2881 cgattcgttt gtcgatgtgt tcgccctgcg gggcaaaccc gtagccgcag cctatcaggc
     2941 agaagcagga caggcacaaa aaccctattc ggatcgcgga caacactcct ggttttttca
     3001 tggcgtttcc tttaagaagc gacgacgagg ttgaccagct ttttggggac gtaaatcact
     3061 ttcagcactt tattcccccc gatgagcgag gcgatttttt catccgccag cgcgtcggcc
     3121 ttgatttttt ccgcgccgca atcgaccggc accgtcatcc ggctgcggag cttgccgttg
     3181 atctggatga cgacggtcat ttcttcctcg gtggcgcagg ccg
//
LOCUS       DAOSIM010000231         3205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_26265_length_3205_cov_11.472063, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000231 DAOSIM010000000
VERSION     DAOSIM010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26265_length_3205_cov_11.472063"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PLR47_09030"
     CDS             <1..704
                     /locus_tag="PLR47_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOG13130.1"
                     /translation="ALRVGVFGAEPWSEQMRAEIEKAMNLKAFNIYGLSEIMGPGVAM
                     ECDEARDGMHIFEDHFLVETIDPETGEVLPPGAEGELVFTTLTKEAFPLIRYRTRDIS
                     RLNPAPCRCGRTHYRMGRVMGRSDDMLIIRGVNVFPSQIEAVLVGIEGLEPHYQLIVD
                     REGTLDTLEVQVELTEKQFDGADEVKVLQRLENQLKKDMKDYLGITARIKLVEPKTIQ
                     RSEGKASRVIDKRKL"
     gene            complement(798..2624)
                     /locus_tag="PLR47_09035"
     CDS             complement(798..2624)
                     /locus_tag="PLR47_09035"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOG13131.1"
                     /translation="MQSNIAKAFGRTVLIGSGAIGTCLRGTVGIGDEPVELLNLRSPE
                     TVKNLHAAYRDAGSQILVTNTFAANALVLGEAGAADRCDDVNRAGVALAREAGGPEGL
                     VWASVGPLGLGLRLEDYPDAALEEIYRRQCLALTEADALLLETFAEPREAKAAMAAAA
                     ATGLPVIFQIGNTGGGGQRWERVDRLLDEAHRAGVIAVGTNCHHPNEIVRVASYIAAH
                     TNLPVTASANAGHPQIERGAITYEYSPEAFASFALELAAAGVAVIGGCCGTTPEHIRK
                     LSEALGGRPVAARPQPAVFAEPVGPAVRQAGEAANPIRSLLAQKPFIISVEIRAERTR
                     SLEEIVQGAEVVAQAGADMFDVPDNPGATVGRDAMVTAARLQSALDVPSIFHLGVTQS
                     NLMRLHSSLIGGWDLGVRGLIAVTGDAPSMGHLGSLAHRVMDVRSSVELLRLIRSLRE
                     GKIINGEGLADPPDFCAGCAIARPIPAQIKWLKTKIDAGAEFVFSQPVFALDDFHRLR
                     DALAGLQIRFFPGIMPLTSRRNAAFLAGGRIPGIVVSSEVAAGFERYESPEDQRRFGL
                     DRACELAAGIAAEASGLYLIMPFGKSCYEDAVRIVREVRKGK"
BASE COUNT          668 a          995 c          940 g          602 t
ORIGIN      
        1 tggcgcttcg cgtcggcgtt ttcggcgcgg agccctggag cgaacaaatg cgggccgaga
       61 tcgaaaaagc gatgaaccta aaggccttca acatctacgg cctgtccgaa atcatgggtc
      121 cgggcgtggc catggaatgc gacgaggcgc gcgacggcat gcacatcttt gaagatcatt
      181 ttctcgtgga gaccatcgat cccgaaacgg gagaagtcct gccgcccggc gccgaagggg
      241 aactggtttt caccacgctg accaaagagg ccttcccgct catccgctac cggacgcggg
      301 acatttcccg cctgaacccg gcgccctgcc gctgcgggcg gacgcattac cgcatggggc
      361 gcgtcatggg cagaagcgac gatatgctga tcatccgcgg cgtcaatgtt ttcccctcgc
      421 agattgaagc cgtgctggtc ggcatcgaag gtctggagcc gcactaccag ttgatcgtgg
      481 accgggaagg aacgctggac acgctggaag ttcaggtgga attgacggaa aagcaattcg
      541 acggcgccga tgaagtcaag gtgctgcagc gtctggagaa tcaactgaaa aaggacatga
      601 aggattatct gggcatcacc gccaggatca aactggtcga gcccaaaacc attcagcgct
      661 ccgaaggcaa ggccagccgc gtgatcgata agagaaaatt gtgaagagcg catgacacat
      721 agctgacagc tcacggctca tcgtggatgg tgaatagctg accgtgacac catccactat
      781 ccactatcca ccatccacta ttttcccttc ctcacctctc ggacgatgcg gacggcgtct
      841 tcgtagcagc ttttgccgaa cggcatgatg agataaaggc cggaagcctc cgcggcaatc
      901 cccgctgcaa gctcgcaggc tcggtccagg ccgaagcggc gctgatcttc aggcgactcg
      961 taccgttcaa aacccgccgc gacttctgaa gaaacaacta tgcccggaat ccgtccgccg
     1021 gccagaaaag ctgcgttgcg ccggctcgta agcggcataa tgccggggaa aaagcggatc
     1081 tgcagccccg caagcgcgtc gcgcagtctg tggaagtcgt caagcgcaaa gaccggctgg
     1141 gaaaaaacaa actcggcgcc ggcgtcgatc ttggttttga gccacttgat ctgcgcggga
     1201 atgggccggg caatcgcgca gccggcgcaa aagtccggcg gatcggccag gccctcgccg
     1261 ttgatgattt tgccttcgcg cagggagcgg atcagccgca ggagctccac ggagctgcgc
     1321 acgtccatca cgcggtgggc aagactcccc agatgtccca tggaaggcgc gtcgcccgtc
     1381 accgcgataa gcccccgcac tcccagatcc cagccgccga tgagcgatga gtgcaggcgc
     1441 atcagattgg actgcgtcac cccgagatgg aagatggagg gaacatccag cgcggactga
     1501 aggcgggcgg cggtcaccat cgcatcgcgc ccgaccgtcg cgccgggatt gtccggcacg
     1561 tcgaacatat ccgccccggc ttgagccacg acctcggcac cctgcacgat ttcttcgaga
     1621 gaccgcgtgc gctcggcacg gatctccaca ctgatgataa acggcttttg cgccagcagc
     1681 gagcgaatgg gattggccgc ttcgcctgcc tgtctgacgg cggggccgac cggttcggcg
     1741 aaaacggcag gctgcggacg cgccgcaacc ggccggccgc ccaatgcttc ggatagtttg
     1801 cgaatgtgtt ccggcgtcgt gccgcagcag ccgccgatga ccgcgacgcc tgcggcggcc
     1861 agctcaagcg caaatgaggc aaatgcctcc ggcgaatatt cgtaggtgat agcgccgcgc
     1921 tcaatttgcg gatgtccggc gttggccgac gcggtgacgg gcagattggt atgcgcggca
     1981 atgtaggagg cgacacggac gatttcattc ggatggtggc agttggttcc cacggcaatg
     2041 acgccggccc ggtgcgcctc gtcaagcaga cggtccactc gttcccatct ctgccctccc
     2101 ccgccggtgt tgccgatctg aaaaatgacg ggcaggcccg tcgcggcggc cgcggccatg
     2161 gcggctttgg cttcgcgcgg ctcggcaaac gtctccagga gcagcgcatc ggcttcggtg
     2221 agagcaaggc actgtctccg atagatctcc tccagcgccg cgtccggata atcctccagg
     2281 cgaagcccca ggcccagagg cccgaccgaa gcccagacca ggccttccgg accgcctgcc
     2341 tccctcgcca gcgccacgcc cgcgcggttg acgtcgtcgc accgatcggc tgcgcccgcc
     2401 tcgcccagaa ccagagcgtt tgccgcaaac gtgttggtca ccagaatctg tgatcccgca
     2461 tcccggtaag ccgcgtgcag gtttttgacg gtttcggggc ttctgagatt gagaagctcc
     2521 accggctcat cgccgattcc aaccgtcccg cgcaggcagg tgccgattgc cccgctgccg
     2581 atcagcacgg tgcgtccgaa ggcctttgca atatttgatt gcatatccat ctccttaccg
     2641 ggggattgca ggccacaatt gctgccggtt cggtcctcta tatatcacat cagtgaaatt
     2701 tttgaatggt gaaggcggat gaaacgatta tctttatccg gcaccgatcc gcaggacacc
     2761 cgcacaatac tgaccgactt atctgtaaac ttaaatactt aagggcgtcc ccttagcccg
     2821 caggacaccc gcacaatact gaccgactta tctgtaaact taaatactta agggtgtccc
     2881 cttagcccgc ttttctggat tccctccttc gcgggcatgg caaggtaaaa agttcaccct
     2941 ccctgaatcc ctcccatcaa gggagggaac ttacagcctc ggaacggttt aaaaaccgtt
     3001 ccctacatat ttctgtccta aaagcctgga gcggtcgcga ccgttcctta cgccctacat
     3061 gaaactttct tcggggatat cgatcgcgct gcggtcgccg tacttcatgg tcttcagata
     3121 tccgaagatg tcgggaacat gattcatgac atagaaccgg gccgtggcga ttttgccctt
     3181 gtagaaatcg gcgtcgattt cgcct
//
LOCUS       DAOSIM010000232         3204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_26275_length_3204_cov_6.772626, whole genome shotgun sequence.
ACCESSION   DAOSIM010000232 DAOSIM010000000
VERSION     DAOSIM010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26275_length_3204_cov_6.772626"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PLR47_09040"
     CDS             <1..433
                     /locus_tag="PLR47_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOG13132.1"
                     /translation="HMAGMVHDLGKISVPSEILSKPTRLTDLERRLIKTHSQAGYDIL
                     KDIEFPWPIARIVLEHHERMDGSGYPNGLKGDQVLPESRILAVADVVEAMASHRPYRA
                     GLGIEAALAEIEANRGTLYDPVVADVCLKLFREKGYRLPES"
     gene            complement(508..990)
                     /locus_tag="PLR47_09045"
     CDS             complement(508..990)
                     /locus_tag="PLR47_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13133.1"
                     /translation="MRDRFGNFIKSVSVCLLLAMMGCDGYTIIPKQEVSGIPNAPYEQ
                     IIKLENPEGAPDRTLAGVVHATGKATVCTDYPRQLVLKSLDDLEKPEKQAFAHFQNYA
                     IHDGDETFGYVSLPVEYGANLWRRDTDPVCKYSVQILPPRHRPGQTDYQDAVQGSTSW
                     "
     gene            1209..1712
                     /locus_tag="PLR47_09050"
     CDS             1209..1712
                     /locus_tag="PLR47_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13134.1"
                     /translation="MKVFKIKFGKSGENADAFGIRFLGDFEGKVGRNGSFSEIIPAAD
                     SAPMIMHQRITIWFTFERVRPFDTVSGFVRQLTELLKEKDYIVKYSFIDGLFNTTVMN
                     DPNPTEGLSDQKVPGNAAAGFSVTAEKQSAKLKFTANEIVSVGEAAAKLGLKIYGRKL
                     ERVSVNR"
     gene            complement(1845..2063)
                     /locus_tag="PLR47_09055"
     CDS             complement(1845..2063)
                     /locus_tag="PLR47_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOG13135.1"
                     /translation="MRSALSVRLPEKMALELDAVGRATGRNKSDIVKESLGEFLWEAR
                     FRRLKKRLSPKAKAAGLITNGNVFKAIS"
     gene            complement(2339..2620)
                     /locus_tag="PLR47_09060"
     CDS             complement(2339..2620)
                     /locus_tag="PLR47_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HOG13136.1"
                     /translation="MPVKRCLLNGRTIGSLNDLYDQLSKKLFLPDHFGRNLDALWDVL
                     TADVPGPFKIVWKQAGLSKKSMGGDFNRAVKLLRDLEKERDDFQLTLEP"
     gene            complement(2611..3105)
                     /locus_tag="PLR47_09065"
     CDS             complement(2611..3105)
                     /locus_tag="PLR47_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HOG13137.1"
                     /translation="MRVTTASAAMMRWTALAAALLLLFLCPLVLTQACAASCEKVVRS
                     LNERLAPAIDERELVEVLRVLNRTQNKKLPAKFVSKQTAQARGWKPGKDLWSIAVLRG
                     CSIGGDPFWNREERLPEKKWREADLDYKGGRRGPKRLVFSRDGARCVTVDHYRTFTEV
                     LPCR"
BASE COUNT          683 a          907 c          875 g          739 t
ORIGIN      
        1 tcatatggcg ggcatggtcc atgatctggg caaaatctcc gttccctccg agattctcag
       61 caaacccacc aggctgaccg acctggagcg ccggctcatc aaaacccact cgcaggccgg
      121 ctatgacatc ctcaaggaca tcgagtttcc ctggcccatt gcgaggatcg ttctggaaca
      181 tcatgaacgg atggacggtt ccggttatcc caacggcctt aaaggggatc aggtgcttcc
      241 ggagtcccgc atcctggccg ttgcggatgt cgtggaagcg atggcctctc atcgtcccta
      301 ccgtgccggg cttggcatcg aagccgccct ggccgagatc gaagcgaacc gggggactct
      361 ctatgacccg gttgtggcgg atgtctgtct gaaattgttt cgcgaaaagg gctatcgcct
      421 gcccgaatcc tgaaaatcaa tgcttccgat ccggcattcg ctgaagcagc ccctccggtc
      481 gtccggagtg gaaacgcttc tcgcccgcta ccagcttgtt gagccctgga ccgcgtcctg
      541 atagtcggtc tgcccgggcc ggtgccgggg cggaaggatt tgcacggagt atttgcagac
      601 cgggtctgtg tccctgcgcc agagattcgc cccgtactcc acgggaaggg aaacatagcc
      661 gaacgtttca tcgccgtcgt gaatcgcgta attctggaag tgcgcgaaag cctgcttctc
      721 cggtttttcc agatcgtcga ggcttttgag caccagctgc cgcggataat ccgtgcacac
      781 ggttgctttc cccgtggcgt ggacgacgcc ggccagcgtt ctgtcgggag ccccctcggg
      841 attttccagc ttgatgattt gttcgtaggg agcattggga atcccggaga cttcctgctt
      901 ggggataatg gtatagccgt cgcagcccat catcgccaac aaaagacaca ccgaaacact
      961 tttgatgaaa tttccaaagc gatctctcat ctttcccttc cgcctttccc ggaagcgggc
     1021 gccaaagccg atccggccgg cgccctttcc gatgccgtca ggctactatc gccgggaagg
     1081 cttgtcaagg acggccgaag acatggccag acaatttcct gaaatacacg tttttgcctt
     1141 cggctttttt gtggtatagt gccgccgctt tgcggcaaaa aactcctgcg cgatacggaa
     1201 cggaaaacat gaaagtattc aaaatcaaat ttgggaagag cggcgaaaac gcagatgctt
     1261 tcggaatacg ttttctgggg gattttgagg gcaaggtggg ccggaacggc agcttcagcg
     1321 aaattattcc cgcagcggat tcggcgccca tgatcatgca tcaaaggatt acgatctggt
     1381 ttacgtttga aagagtcaga ccgttcgaca ccgtctccgg ctttgtccgc cagttgacgg
     1441 aactactgaa agagaaagat tacatcgtca aatactcctt tattgacgga ttgttcaata
     1501 cgaccgtgat gaatgacccg aatcccacgg aggggctttc cgaccagaag gttcccggca
     1561 acgccgccgc cggtttttcc gtcacagccg aaaaacagag cgccaaattg aaatttacgg
     1621 caaatgagat cgtgtcggtc ggcgaagcgg ccgcgaaatt gggcctcaag atttatggca
     1681 gaaagctgga gagagtcagt gtcaaccggt aaattggccc ggcctgtgtt gtaaattcat
     1741 gccctcttcc gttgggtgac atttgaaatt ctcgttctaa aatgtaacca tgaatgcgga
     1801 aatccggcgc gccttccttc ccccttgtat tgacgcagca attttcacga tatggccttg
     1861 aaaacgttgc cattcgtgat caggcctgcc gcttttgctt tggggctaag gcgtttcttc
     1921 agccggcgga atctggcctc ccacaaaaac tcccccagcg actcttttac gatatcgctt
     1981 ttatttcttc ctgttgcacg gccaacggca tccagttcca acgccatttt ctccggcagt
     2041 ctgacagaca gagcgcttct catcctgcgc ccggaatatt attttcggat catccggttg
     2101 atgcgtacgt aaacatcgta cgtaatagaa ggaagaacga aaaatgcaga tgaatcttaa
     2161 aaattgcctt ccggtatgcc ggataacgcg cttgaatatg gaggggcgtc aattcatttg
     2221 tgaattcata atgtctttgg gcattgccgc agccaacccc gggaatggag attcgtcatg
     2281 tttctgttgt ttaacttgaa gcccttgaac atgcccgtgt cgatgatcgg gcaaaaggct
     2341 acggctcgag ggtcaattga aaatcgtctc tttccttttc caggtcccga agcagtttca
     2401 cggcccggtt aaaatccccg cccatggatt tcttggaaag gccggcctgt ttccagacga
     2461 ttttgaaggg gccgggaacg tccgcggtca gcacgtccca cagcgcgtcg aggttgcggc
     2521 cgaagtgatc gggcaggaag agctttttgg aaagctggtc atagaggtcg ttcagcgagc
     2581 cgatggtgcg tccgtttaac aggcatcttt ttaccggcat gggagcacct ccgtaaaagt
     2641 ccggtagtgg tccacggtta cgcatcgcgc tccgtctcgg gaaaagacca gacgcttcgg
     2701 gcccctgcgt ccacccttgt agtccagatc ggcttcccgc catttttttt caggaagcct
     2761 ttcttcccgg ttccaaaacg gatcgccgcc gatgctgcag ccccggagca ccgcgatcga
     2821 ccacaggtcc tttccgggct tccatccccg cgcctgggcc gtctgtttgc tcacgaattt
     2881 ggccggaagc tttttgtttt gcgtccggtt cagaacacgg agcacctcaa ccagctcccg
     2941 ctcgtcgatg gccggggcca gccgctcatt caggctccgg acgactttct cgcaggacgc
     3001 tgcgcaggct tgcgtcagca ccagagggca caggaacaaa agaagaaggg ccgccgcgag
     3061 agccgtccat ctcatcatag ccgctgaagc agtcgttacg cgcattcgct gtagccgcag
     3121 aggcggcaga cggcgcagcc gccctcgtgc tcgatgatgc cgccgcagtc cggacaggcg
     3181 cctttgagga aaacgcgctt caca
//
LOCUS       DAOSIM010000233         3182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_26498_length_3182_cov_7.696834, whole genome shotgun sequence.
ACCESSION   DAOSIM010000233 DAOSIM010000000
VERSION     DAOSIM010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26498_length_3182_cov_7.696834"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..716)
                     /locus_tag="PLR47_09070"
     CDS             complement(237..716)
                     /locus_tag="PLR47_09070"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOG13138.1"
                     /translation="MAGIQVGDVFGDFSLKSHTEAAVDTAQFAGQKILLSFHPLAWTE
                     VCAKQMQSLEARLDDFRNHNTVPLGISVDSAPCKKAWAKNLGIEKVDLLADFWPHGGL
                     AARLGLFIDKLGFSERANIILDEKRKAVFVKVYPLRELPDIQEMMDFLRITQLPNQR"
     gene            complement(793..1491)
                     /locus_tag="PLR47_09075"
     CDS             complement(793..1491)
                     /locus_tag="PLR47_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HOG13139.1"
                     /translation="MLQIVQFRYGDNLAYLLYGPTEALAIDGGAWQEILAFLEQNRLM
                     LAYVTNTHRHYDHTPGDDHLLRKTKAGFLDCTAFADGEKILIDGEPVIAYRTPGHSMD
                     SVCFHAGQNLVTGDTLFNATVGNCFTGDLKGFFDSIQRLMALPDDTRIFAGHDYVRDS
                     LAFARHLEPDNPEIERFRQTCDPHFLFSTLAQEKTMNPYLRFNEEPILRLLQKRGLPH
                     ATEWERWQSLMAIE"
     gene            complement(1502..2041)
                     /locus_tag="PLR47_09080"
     CDS             complement(1502..2041)
                     /locus_tag="PLR47_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOG13140.1"
                     /translation="MLSRSAAVLVLVDFQGNLAQSMDRKEELFANTVKLIRGFQILNL
                     PVVATEQTPEKLGTTIPLIARELGSGKPIAKETFSCWANPSFRDELESLTRRHVVLAG
                     IESHVCVYQTALDLLRNGYTVHLAADAVSSRKPENRRIGIDAIRSGGAHLTSAEMVLF
                     ELLGSAADSKSRDIFKIVK"
     gene            2148..2927
                     /locus_tag="PLR47_09085"
     CDS             2148..2927
                     /locus_tag="PLR47_09085"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOG13141.1"
                     /translation="MTLPANEKILLHEYREGRFAEVELPFSAEYALTLNINGSPYVTM
                     ACSGKDLREHVTGYLITEGIISGADQIARLEVHEKDLAVDVFLMNDRIVAERLEQIKT
                     ISAAGGRTRTRLPGEELVRTRLPGVGAEVILGAMSEFLSYSREHEATHGVHSAALYSL
                     EGGRLIFFDEIGRHNAIDKVIGRAAIQGISLEDKMICSTGRISSEIAFKIIHARAPVL
                     ISRASPTTLSVSLLRRFNILTVIRAVNGRFFVVNGKESIRV"
     gene            complement(2975..>3182)
                     /locus_tag="PLR47_09090"
     CDS             complement(2975..>3182)
                     /locus_tag="PLR47_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOG13142.1"
                     /translation="IAKYIEKNGEGIQHMALRVDDLEAALAELKAKGVRLIDEKPRYG
                     AGGAKIAFVHPKSTGGILLELSER"
BASE COUNT          627 a          876 c          948 g          731 t
ORIGIN      
        1 atgctgcata cccggctcgt gcgcgggtgg cgcggccggc gcatggccgt gatgtcgatg
       61 gtcggcttcg gctgcatgct cttcacgtat ctgggggtga atttattgcc ctctttgcac
      121 agctacatga gctgacgcca ctcccaactc aaagcgctat ctttgttggc tgagtcgtcg
      181 gcatcctcaa cgtatataaa aatacgcctc gtcgcctcct ctcagccgcc gcgatatcat
      241 ctttgatttg ggagctgcgt tattcttaag aaatccatca tttcctggat gtccggcagt
      301 tcccgaaggg gatagacttt gacgaagacg gcttttcgct tctcgtccag aatgatgttg
      361 gcccgctcgg agaagccgag tttgtcgatg aagaggccca gcctcgcggc gaggccgccg
      421 tggggccaga agtccgccag aagatcgact ttttcgatgc cgagattttt agcccaggct
      481 tttttgcagg gagcgctgtc cacggagatt cccaacggaa cggtgttgtg attccggaag
      541 tcgtccaggc gggcttccag cgactgcatc tgcttcgcgc agacttccgt ccaggccagc
      601 ggatgaaagg agagcagaat tttttggccg gcaaactgcg ccgtatccac cgcggcttcc
      661 gtgtgactct ttaacgagaa gtctccaaac acatctccga cttgaattcc ggccatgatg
      721 cttctccttt cgtatttcgt cgttcgtatc tcgtgtctcg cgaaccctga accctgaatt
      781 gttgaacctg tttcactcga tcgccatcag cgactgccag cgctcccatt cggtggcgtg
      841 cggaaggccg cgcttctgca gcagccgaag gataggctcc tcgttgaatc tcaggtaagg
      901 attcatcgtc ttttcctggg cgagagtgga gaagagaaaa tgcggatcgc aggtttgccg
      961 gaaacgctcg atttcgggat tgtccggctc caaatgccgg gcgaaagcta gcgaatccct
     1021 cacataatca tggccggcga aaatgcgcgt gtcgtcgggc agggccatca gacgctgaat
     1081 ggaatcgaaa aatcctttaa gatcgccggt aaaacagttg ccgactgtcg cgttgaagag
     1141 agtgtcgccg gtcacgaggt tttgccccgc gtgaaagcat acggaatcca tggaatggcc
     1201 gggtgtccga tatgcgatga ccggttcgcc gtcgatgagg attttttctc cgtcggcaaa
     1261 cgccgtacag tcaagaaacc cggcttttgt ctttctcagc aggtgatcgt cgccgggcgt
     1321 gtggtcatag tgccggtgcg tgttggtgac gtacgcgagc ataagccggt tctgctcgag
     1381 aaacgcgagg atttcctgcc aggcgccgcc gtcgatcgcc agggcctccg tcgggccgta
     1441 cagcaggtag gccagattgt ctccgtagcg gaattgtacg atctggagca tgccgttttc
     1501 cctattttac gattttaaag atgtctctgg atttggaatc ggcggcgctg ccgagcagtt
     1561 caaacagaac catctccgcc gacgtgagat gagcgccgcc gctcctgatc gcgtcgatgc
     1621 cgatccggcg gttttccggc ttgcgcgacg aaacggcgtc ggcggccaga tgaaccgtgt
     1681 agccgttgcg aagaaggtcc agcgccgtct ggtaaacaca gacgtggctt tcaatgccgg
     1741 ccaggacgac atgccggcgg gtgagggatt cgagctcgtc gcggaatgag ggattggccc
     1801 agcagctgaa cgtctccttg gcaatgggtt ttccgcttcc cagctcccgg gcgatcaggg
     1861 gaatcgtcgt ccccagtttt tccggtgtct gctccgttgc cacaacgggc aggttcagga
     1921 tctgaaatcc ccggatcagc ttgacggtat tggcaaaaag ttcttccttg cggtccatgg
     1981 actgggccag gttgccctga aaatcgacca gaaccagcac ggcggcgctt cggctgagca
     2041 tgtttccccc ttttatggct gcttttgccg aacaatagtg aatttgcttt ggggttgtca
     2101 atcattatta ttgctggtaa taaggctaaa aagcctggag ataaaacatg acgctgccgg
     2161 ccaatgaaaa gattcttctg catgaatacc gggaaggccg ctttgcggaa gtggagctgc
     2221 ccttttccgc ggaatatgcg ctgacgctga atatcaacgg cagcccctac gtcacgatgg
     2281 cctgctccgg aaaggatctg cgggagcacg tgaccgggta tctgatcacg gaaggaatca
     2341 tttcgggcgc ggatcagatc gcaaggctgg aggttcatga aaaagatctg gccgtcgacg
     2401 tctttctcat gaatgaccgg atcgtcgcgg aaaggctcga acagatcaaa accatttccg
     2461 cggcgggggg aaggacccgc acgcggctgc ccggggagga actggtccgc acgcggctgc
     2521 ccggggtggg ggcggaggtc atcctggggg ccatgagcga gtttctgtcc tattcgcggg
     2581 agcatgaggc cacgcacggc gttcacagcg cggcgcttta ttcgctggag ggcgggcggc
     2641 tgatcttctt cgacgaaatc ggccgtcaca acgccatcga caaggtgatc ggtcgcgcgg
     2701 ccattcaggg catcagtctg gaagacaaga tgatctgctc gacggggcgc atctcgagtg
     2761 agatcgcctt caaaatcatc cacgcccgcg cgccggttct gatttcgcgc gcctctccga
     2821 ccacgctgag cgtgagcctt ctgcgccggt ttaatattct gaccgtcatc cgcgccgtca
     2881 acggccggtt ctttgtcgta aacggcaaag aaagcattcg ggtatagcgt ccgcttcggg
     2941 cgcttcagga tgccgcccgg gagcgcgagg cgttttatct ttcgctcagt tccagcaaaa
     3001 tgccgccggt tgatttggga tgaacaaagg cgattttcgc gccgcctgcg ccatagcggg
     3061 gcttctcgtc gatcaggcga acgcctttgg ctttgagttc ggccaacgcc gcttcaagat
     3121 catccacccg cagggccatg tgctgaatgc cctcgccgtt tttttcaatg tatttggcaa
     3181 tg
//
LOCUS       DAOSIM010000234         3162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_26708_length_3162_cov_17.982620, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000234 DAOSIM010000000
VERSION     DAOSIM010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26708_length_3162_cov_17.982620"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /gene="thrC"
                     /locus_tag="PLR47_09095"
     CDS             complement(<1..1035)
                     /gene="thrC"
                     /locus_tag="PLR47_09095"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOG13143.1"
                     /translation="MPIRYYSTNRHVKQAAGITPFTGSVSFREALLAGQSPDEGLFVP
                     DAIPRLSMKDMVALRDQPYWQAALLVARAFLSDEFSPDVLEAIVKDAYNFPVPLEAVY
                     PRAFILRLDQGPTASFKDFAARMMARLMSHCRPEGERLNILVATSGDTGSAVGEAYKG
                     VDGIDVTILYPADEVSLMQKKQLDTIGGNVRAMCVDGKFDDCQNLVKEAFADKDLARF
                     NLSSANSINFGRILPQIVYYVYAYAQLAGAGEEVVVSVPSGNFGNALGCEYARRMGLP
                     VAKLIMPTNENDEFPAFLETGIYTKLSPSRACISNAMNVGHPSNLARFFELYGGTVDR
                     TGMVHRYPDLE"
     gene            1338..2390
                     /gene="selD"
                     /locus_tag="PLR47_09100"
     CDS             1338..2390
                     /gene="selD"
                     /locus_tag="PLR47_09100"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1389..1391,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOG13144.1"
                     /translation="MEEKKTVRLTETVSSAGUACKIGPGDLSDILSDLPLISDPKLVT
                     GYEHAEDAGVYKITDDLALVQTVDFFTPTVDDPFTFGQIAAVNALNDIYAMGGTPLTA
                     MNIVCFPIKTMDKSVLREVLRGGLDKMREAGVLLVGGHSVEDNEIKYGLSVTGTIHPD
                     RVLFNRGARPGDRLILTKPLGTGIVATAIKAGAAGQALVEQATRCMTTLNKKAAELMI
                     AAGDIHSCTDITGFGFLGHACEMIEGSDVGLRIEASRIPILEGVLDLTQTGYLPGGLY
                     RNRNYRLHQVEKAPSCPDWIFDVCFDPQTAGGLLFSLPSEKAEPLAAAMRRAGITDAV
                     VVGDVVSDDPGKILME"
     gene            2397..>3162
                     /locus_tag="PLR47_09105"
     CDS             2397..>3162
                     /locus_tag="PLR47_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13145.1"
                     /translation="MNRLPFKSETERNVRKLLELAHSDDSLLLLMYGSPDPDAIASAM
                     ALREILLRTKGLSKWVFAATEPIRRQQNRELAQAMRLNIRLLNQVDAGAYRLVALVDA
                     QPSFFGDSLPAREPNIVLDHHPREGNWQAELADIRPLYGALSTILTEYLLVARVRIPR
                     NLHTALLYGIKTDTDNFDRETIAEDIRAYTYHSRHANLRLIRRIELNQTPRSYLKYFD
                     HAFHSMTHFRGRRIGFLGYVESPDVCVQVADFYLHVI"
BASE COUNT          663 a          901 c          920 g          678 t
ORIGIN      
        1 ctccaggtcg ggatagcggt gaaccattcc cgtgcggtcc acggtgccgc cgtacaattc
       61 aaagaatcgc gccaagttgc tgggatgccc cacgttcatg gcgttggaaa tgcaggcgcg
      121 cgacggcgac aatttcgtgt agattccggt ttccagaaaa gcgggaaact cgtcgttttc
      181 attggtgggc atgatcagtt tggccaccgg caatcccatg cggcgggcgt attcgcagcc
      241 cagggcgttt ccgaaattgc cggaagggac ggagaccacc acctcctcgc cggcgccggc
      301 cagttgggcg taggcataga catagtaaac gatctgcggc agaatgcgcc cgaagttgat
      361 cgagttggcc gacgacagat tgaaccttgc cagatctttg tcggcaaacg cttctttgac
      421 cagattctga cagtcgtcga attttccgtc cacgcacatg gcccgcacgt tgccgccgat
      481 ggtgtcgagc tgctttttct gcatcaggct cacttcatcc gccggataga ggatggtcac
      541 atcaatgccg tcaacgccct tgtaggcttc tccgactgcg cttcccgtat cgccggacgt
      601 cgccaccaga atattgagcc tctctccttc aggccggcag tggctcatga gccgcgccat
      661 catgcgggcg gcgaaatcct tgaaggacgc cgtggggccc tggtccaacc gaaggataaa
      721 ggcgcgcgga tacaccgcct ccagcgggac gggaaagttg tacgcgtcct tcacaatcgc
      781 ctcgaggaca tcgggtgaaa actcatcgga gagaaacgcc cgggcgacca gcagcgcggc
      841 ctgccagtaa ggttggtcgc gcagcgcaac catatccttc atggaaagcc gcggaatggc
      901 atcgggaaca aaaagtccct catccggcga ctgcccggcc agcagcgctt cccgaaaaga
      961 tacgctgccg gtaaagggag taattcccgc agcctgtttt acatgacgat tggtgctgta
     1021 gtagcgaatg ggcatgcccg ttgtcccttt caaaggtgat tttctataat ctgattggcg
     1081 caaataaatc aacaaaaaac aataccctcc tgtcgggtgt ttgcctgggc gatctgccgg
     1141 tacggtcatg gacggcgttt tgtcttctcc gaagggcctg cggtttttcc ggcgggcaaa
     1201 aataaattgc aaaaccggct gttcggaatc cggaaagctg tcttttccga aatccatttg
     1261 gaaaatagcg gcttctatgc taaaaaacca aacgcttcag gaatgggaag cgcaaattct
     1321 ttttcgggtt gaaaagcatg gaagaaaaaa aaaccgttcg tttgacggaa accgtatcga
     1381 gcgcgggttg agcctgcaag atcggtccgg gggacctgtc cgacattctc agcgatttgc
     1441 cgctcatctc cgatccgaaa ctggtgaccg ggtatgagca tgcggaagac gcgggcgtgt
     1501 ataaaatcac cgacgacctg gcgctggtgc agaccgtgga ttttttcacc ccgaccgtgg
     1561 atgatccctt caccttcggt caaatcgccg ccgtcaatgc cttaaacgac atttacgcga
     1621 tggggggcac tccgctcacc gccatgaaca tcgtttgttt tcccattaag acgatggaca
     1681 aatcggtgct gcgcgaggtt ttgcgcggag ggctggacaa gatgcgcgaa gccggcgttc
     1741 tcttggtcgg tggccacagc gtggaagaca acgaaatcaa gtatggtctg tcggtgaccg
     1801 gcacgattca tccggaccgc gtgctgttca accgcggcgc ccgccccggt gaccggctca
     1861 ttctgaccaa gccgctgggt acgggcattg tcgcgacggc catcaaggcg ggggcggccg
     1921 ggcaggcgct tgtcgaacag gcaacgcgct gcatgacgac gctcaataaa aaagcggccg
     1981 agctgatgat cgcggcggga gacattcatt cctgcacgga tatcacgggc ttcggttttc
     2041 tcggacacgc ctgtgaaatg attgaaggca gcgatgtcgg cctgcggatc gaggcgtcca
     2101 gaattccgat tctggagggc gtgctggatc tcactcaaac cggctacctt cccggcggtc
     2161 tttaccgcaa ccggaactac cgccttcatc aggtcgagaa agctccgtct tgtccggact
     2221 ggatttttga cgtctgcttt gatccgcaga cggcgggagg tctgttgttc agcctgcctt
     2281 ccgagaaggc ggagcctctt gcggctgcca tgcgccgggc gggcatcacg gacgcggtcg
     2341 tcgtgggcga tgtggtcagt gatgacccgg gtaaaattct catggagtaa agtcctttga
     2401 atcgcttgcc cttcaaaagt gagactgagc ggaatgtcag aaagctcctc gagctcgcgc
     2461 attcggacga ttcgctgctg ttgctgatgt acggcagccc ggatccggac gcaatcgctt
     2521 cggccatggc gcttcgcgaa atcctccttc ggaccaaagg tctttccaaa tgggtttttg
     2581 cggcgacgga gccgatccgc cgtcagcaga accgggaact ggcgcaggcg atgcgcctca
     2641 acattcggct tctgaatcag gttgatgccg gcgcctaccg gctagttgcg ctggtggacg
     2701 cacagccttc tttttttgga gattccctcc cggcccgcga gccgaatatc gtcctggatc
     2761 atcatccccg cgagggaaac tggcaggccg aacttgccga cattcggccc ttgtacggcg
     2821 cgctctcgac gattttaacg gaatacctgc tggtcgcgcg ggtgcgcatt ccccgcaatc
     2881 tgcacacggc gctgctttac ggcatcaaga cggacacgga taatttcgac cgcgaaacga
     2941 ttgccgaaga cattcgcgcc tacacctatc attcccggca cgccaacctg cggctgatcc
     3001 gccggatcga actcaatcag acgccgcgca gctatctgaa atatttcgac cacgcgtttc
     3061 attccatgac acatttccgc ggacgccgca tcggtttcct ggggtacgtg gaaagtccgg
     3121 atgtctgcgt gcaggtggcg gatttttatc tgcacgtgat cg
//
LOCUS       DAOSIM010000235         3154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_26798_length_3154_cov_9.099387, whole genome shotgun sequence.
ACCESSION   DAOSIM010000235 DAOSIM010000000
VERSION     DAOSIM010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26798_length_3154_cov_9.099387"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PLR47_09110"
     CDS             complement(<1..193)
                     /locus_tag="PLR47_09110"
                     /inference="COORDINATES: protein motif:HMM:NF024402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13146.1"
                     /translation="MKTSGKTALLFMLYLSQGLPFGFQATALPALLRERGLSLAAIGF
                     AGALAAPWLLKPLWAPLVDR"
     gene            419..1252
                     /locus_tag="PLR47_09115"
     CDS             419..1252
                     /locus_tag="PLR47_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOG13147.1"
                     /translation="MAEKNLVYRGKSKDVFRITSGAYKGKYRFVFTDRGTGYFENGKP
                     VFDPGYDVVVGEIPGKGAIACRFATYFFQLLKSKGVATHYIDTTSDNEMIVEPAIPLS
                     MKVQGPAFPGSAPLSNLEFTWRNSATGSFWRRYPFIRPCTNLHRVVEAWTKGDSDVLI
                     TMEALAETGALARKEIAQSVKLVQKIADIVSKEFASRNLHVIDGKFELGRLKYGDGKI
                     VLIDEISPDVLRVCRGYKPNGNGDCTRFRACTVTGESDGKRTIKNRNQVSAAELVKIF
                     R"
     gene            complement(1277..1693)
                     /locus_tag="PLR47_09120"
     CDS             complement(1277..1693)
                     /locus_tag="PLR47_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13148.1"
                     /translation="MKISIPDRSLYYKGLMILVRRDREIHEKEKKFMMSIGKKLGFDR
                     KFCAKTMEDILVNHYITDEPPRFSQAGVARSFLLDGLKLSAADGQIHEAEISWLTSVA
                     KQNGLADFWNEELVKFRSSPPAGISGDHFEVTKFQW"
     gene            complement(1713..2114)
                     /locus_tag="PLR47_09125"
     CDS             complement(1713..2114)
                     /locus_tag="PLR47_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein OmpA"
                     /protein_id="HOG13149.1"
                     /translation="MKKWMVPWMIFLFAGCTVSPVLYPNAHLSNVGNDQAQRDIALCR
                     EQADMYVKSDAAKEAAKDTVIGGIGGAVVGGAIGAVDGSFGRGVGMGAAGGAAAGLVS
                     GIIHASQPDPLYINFVNRCLSEKGYEIIGWQ"
     gene            2286..>3154
                     /locus_tag="PLR47_09130"
     CDS             2286..>3154
                     /locus_tag="PLR47_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOG13150.1"
                     /translation="MQIPGPNPFSRQGQRDGLSRWIPLIATLRGYQRGWLWRDLAAGL
                     ALTALLVPVGMSYAAASGLPAIYGLYATIIPLAAYAVFGPSRIMVLGPDSALVGLIAA
                     TILPLSAGNPEKAVALASVLAVLSGLLCIAAGLAKFGFITDLLSKPIRDGYINGIALT
                     VLVGQLPKVLGFSVSPGSFLSEIGELAQGIGSGQIHPIACVIGLSCLAVILGFRRLAP
                     AIPGVLIAVAGATILVSWLPGPVREGIAVIGPLPQGLPQFRIPDVSPAEIGELIAAAV
                     AIALVSFTDMSAVS"
BASE COUNT          632 a          894 c          954 g          674 t
ORIGIN      
        1 agcggtccac cagcggcgcc cacagcggct tgagcagcca gggcgcggcc agcgccccgg
       61 caaaaccgat ggcggcaagc gagaggcccc tttcccgcag gagcgctgga agcgcggtgg
      121 cctgaaagcc gaaggggagg ccctgcgaaa ggtaaagcat gaacaggagt gccgttttgc
      181 cgctggtctt cacatgcgtt ctccaaggga gcctgggcgc gccgggaggt ttccggtgag
      241 ccgggcagac tataaaaaac gggagcctga atgtcaatcg atgatagcaa tcccgcaaag
      301 ccagttttcg tttgacacgt ccggcctttc tttatatgca gcatcctgat ttttttgaac
      361 agggcggcgt ggcacaaaac ggttgcacgg ttgaaaattc attaaggaga ggacactcat
      421 ggcggagaaa aacctggttt accgaggcaa gtcgaaagat gtatttagaa tcacaagcgg
      481 ggcgtacaaa ggaaagtacc gttttgtgtt taccgaccgg ggcaccggct actttgaaaa
      541 cggcaagccg gtttttgatc cgggatacga cgttgtcgtc ggtgaaattc ccggaaaagg
      601 cgccatcgcg tgccggtttg ccacgtattt tttccagctt ctgaaatcca agggcgtcgc
      661 cacgcattat attgatacga cgagcgacaa cgaaatgatc gtggagccgg ccattccgct
      721 gagcatgaag gtgcagggac cggcgtttcc cggttcggct cctctttcca acctggagtt
      781 cacctggagg aacagcgcaa ccggaagctt ctggaggcgg tatcccttca tccggccctg
      841 cacgaacctc caccgggtgg tcgaagcgtg gacgaaaggg gacagcgacg ttctgatcac
      901 gatggaggcg ctggccgaaa cgggcgcgct ggcccgaaag gaaatcgcgc agagcgtgaa
      961 gctggttcaa aaaatcgccg acatcgtttc gaaagaattt gcgtcccgaa atctgcatgt
     1021 catcgacgga aagttcgagc tgggacggct gaaatacggc gacggcaaaa tcgtgctgat
     1081 cgatgaaata tcgcctgacg tcctgcgggt gtgccggggc tacaaaccga acgggaacgg
     1141 ggactgcacg cggttcaggg cctgcaccgt cacgggggaa tcggacggca aacggacgat
     1201 caaaaacaga aatcaggtat cggcggcgga gctggtcaaa attttccggt aggcgtcagc
     1261 gcgggtgcgt cgggcgtcac cattgaaatt ttgtcacctc gaagtgatcg ccggaaattc
     1321 cggcgggggg agaggaccgg aatttcacaa gctcctcatt ccagaaatcg gcaagtccgt
     1381 tttgtttggc cacggaggtg agccaggaga tttcggcttc gtggatttgt ccgtcggccg
     1441 cggagagctt cagaccgtcc aggagaaacg aacgggcgac gccggcttgt gagaagcggg
     1501 gcggctcgtc ggtgatgtag tggttcacca gaatgtcctc catggtcttg gcgcagaatt
     1561 tccggtcaaa tcccagcttt ttcccgatgc tcatcatgaa tttcttttct ttttcatgga
     1621 tctcgcggtc ccggcggacc agaatcatca atcctttata gtaaagactg cgatccggga
     1681 tggaaatttt catgggcgcc gtcctttggc cgtcactgcc atccgatgat ttcatagccc
     1741 ttttcgctga ggcagcgatt gacgaaattg atataaagcg ggtcgggctg agaggcatgg
     1801 atgatcccgc tcaccagtcc cgccgccgca ccgccggccg cgcccatgcc cacgcccctg
     1861 ccgaagcttc cgtccaccgc gccgatcgcg ccgccgacca cggcgccgcc gatgcctccg
     1921 atgacggtgt cttttgcggc ttctttggcc gcgtcggact tgacgtacat gtcggcctgc
     1981 tcccggcaca gggcaatgtc ccgctgggcc tgatcatttc cgacattgct taagtgggcg
     2041 ttggggtaaa gcacggggct caccgtgcag ccggcgaaca aaaaaatcat ccaggggacc
     2101 atccattttt tcatcttgaa acaccctttc tttgccggga cttcggaaaa tgcaatgatg
     2161 ctatacaacg atttgccaaa aatgacaaat gccgggggga gacctcgaaa agggatttca
     2221 tgttgtaaaa accggtgaaa atttataagc tcttcatcat tttcctgagg tgcgccgtgg
     2281 cggctatgca aatcccgggg ccaaatccat tttcacgtca aggacagcgg gacggtcttt
     2341 cacgatggat tcccctgatc gcgaccctgc gcgggtatca gcgcggctgg ctgtggcgcg
     2401 acctggcggc cggattggcg ctgacggccc ttctggtgcc ggtgggaatg agttatgccg
     2461 cggcatcggg cctgcccgcc atttacggac tgtatgccac catcattccg cttgcggcct
     2521 atgccgtctt cggccccagc cgcatcatgg ttctgggacc ggactccgcg ctggtcggcc
     2581 tcatcgccgc gacgattctg ccgctttccg ccggaaaccc ggaaaaggcg gtggcgctgg
     2641 cctccgtgct ggcggttctg tccgggctgt tatgcattgc ggcgggtctg gcaaaattcg
     2701 gcttcatcac cgatcttctc tccaagccca ttcgggacgg ctacatcaac ggcattgcgc
     2761 tgaccgtgct cgtcggccag ttgcccaaag tgctcggctt ttccgtcagc ccgggcagtt
     2821 tcctttcgga aatcggagaa ctggcgcagg gcatcggcag cggacagatt catccgatcg
     2881 cctgcgtcat cggcctttcc tgtctggcgg tgattctcgg attccgccgc ttagcgccgg
     2941 ccattcccgg cgtgcttatc gccgtggcgg gcgcgacgat tctggtttcc tggcttcccg
     3001 gtcccgtccg ggagggaatc gccgtcatcg gtccgctgcc gcagggtctg ccgcaattcc
     3061 ggattcccga tgtgtcgccc gccgaaatcg gtgaactgat tgccgccgcc gttgcgattg
     3121 ccctggtgtc ctttaccgat atgagcgcgg tttc
//
LOCUS       DAOSIM010000236         3111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_27241_length_3111_cov_8.182264, whole genome shotgun sequence.
ACCESSION   DAOSIM010000236 DAOSIM010000000
VERSION     DAOSIM010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27241_length_3111_cov_8.182264"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PLR47_09135"
     CDS             <1..647
                     /locus_tag="PLR47_09135"
                     /inference="COORDINATES: protein motif:HMM:NF014822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HOG13151.1"
                     /translation="FILPYSHDEVVHGKKSLLGKMYGAYDEKFASLRLLLGFMYAHPG
                     KKLLFMGGEFGQFIEWDYRKELDWFLLGYESHDRMRRYVQALNEFYTRTPALYEIEDG
                     WDGFAWLNVNNAKQSAVSFLRRGARPEEVCVCAFNFTPVPVERFVIGLPSKGALREVF
                     SSNEARFGGSGHGAARVIQAVHKEFEGHPYRAAVDLPPLAAVYFELDSNKKGLE"
     gene            644..2125
                     /locus_tag="PLR47_09140"
     CDS             644..2125
                     /locus_tag="PLR47_09140"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOG13152.1"
                     /translation="MNRALQEIPAEKKMKTQPSVLMIGAECAPFAKTGGLADVIGTLT
                     RELKTLEFDVRLMLPFHRIIKHRNRDRVRHLVSFSVDIGGRAQYVGVEQFDWEGIPVY
                     FIDNEHYFGHAIYCGGDFEGEQYAYVSRAAIEALPLIGFSPRILHVNDWHTAMIPMLL
                     KTQYDRLQQGRCRTVLSIHNLGYQGRFHFGFVSHLLGIESRYYHPDNIEYHGDANFLK
                     GGIVYADKLVTVSPTYAREILTAYYGEGLEGILATRAEDLTGILNGIDTNAFNPAADP
                     FIPYPYDASSLSAKQKNKEALIAELGLNLPFDAPIIGMVTRLTKQKGIDLVQRVFDEI
                     IAAGAGFALIGTGDHAYEHFFRGAAHRTGGRAVGVTQFNDPLARRIYAGCDFFLMPSQ
                     FEPCGLSQMIALRYGTLPIVRATGGLRDTVFPYNEYTDEGNGFTFENYNAHDMLHTIR
                     YALGVYENKERLDSLRLRAMGQDLSFQKSALAYAKLYADAAGK"
     gene            2229..>3111
                     /locus_tag="PLR47_09145"
     CDS             2229..>3111
                     /locus_tag="PLR47_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOG13153.1"
                     /translation="MKNRIAKIKGIIEGKLKRHYGRTLENASDEDMFQVTAMSVRDIM
                     LERGVLANETVEKKGQKTLCYLSAEFLMGRALVNNLINLGLLTEYRAVMAEIGYPFEK
                     LEEQEMEAALGNGGLGRLAACFLDSLSTLNLPVTGYGIRYEYGMFRQHIVDGRQTEVP
                     DDWTAKGDIWEIERREEQVEVHFDGTVEEVWTDSGMTCEHRDFQTVLAVPHDMPVIGY
                     ESHTPATLRLWRAECPSGFDLHSFNKGDFVCMVRQKELAESISKVLYPQDNHMTGRML
                     RLRQYYFLASATMQCIVR"
BASE COUNT          680 a          839 c          953 g          639 t
ORIGIN      
        1 acttcattct gccgtattcc cacgacgagg tggttcacgg caaaaaatcc ctgctgggca
       61 aaatgtacgg cgcctacgac gagaaattcg catcgctgcg cctgctgctg ggattcatgt
      121 acgcgcaccc cggaaaaaaa ctgctcttca tgggcggcga gttcgggcaa tttatcgagt
      181 gggattaccg gaaggaactc gactggtttc tgctgggtta tgaaagccat gaccggatgc
      241 gccgctacgt acaggcgctg aacgagtttt acacccgcac cccggcgtta tacgaaatcg
      301 aggacgggtg ggacggattt gcctggctca atgtgaacaa tgcaaagcag agcgccgtga
      361 gttttctgag gcgtggcgcg cgccccgaag aggtctgcgt gtgcgctttc aactttacgc
      421 cggtgccggt ggagcggttt gtaatcgggt tgccgtccaa aggagcgctg cgggaagtct
      481 tcagcagcaa cgaggcgagg tttggaggaa gcgggcacgg cgcggcaagg gtcattcaag
      541 ccgtccataa agaatttgaa ggacatccgt atcgtgcggc cgttgatctg ccgccgcttg
      601 cggccgtcta ttttgaattg gattcaaata aaaagggttt ggaatgaaca gagcattgca
      661 ggaaattccg gctgaaaaaa agatgaagac ccagccttcg gttctgatga tcggggcgga
      721 atgcgcgccg tttgccaaaa cgggggggct ggcggatgtc atcgggacgc tgacgaggga
      781 gctgaaaacg ctggaattcg atgttcggct gatgctgccg tttcatcgca tcatcaagca
      841 ccggaaccgg gaccgcgtgc gccacctggt cagcttcagc gtggacatcg gcgggcgagc
      901 ccagtatgtg ggagtcgagc agttcgactg ggagggcatc cccgtttact tcatcgacaa
      961 cgagcattac ttcggacacg ccatttactg cggcggcgat ttcgagggcg aacagtacgc
     1021 atatgtttcg cgggcggcga tcgaggcgct cccgctgatc ggcttttcgc cccggattct
     1081 gcacgtcaac gactggcaca cggccatgat tcccatgctg ctgaaaaccc agtacgaccg
     1141 cctgcaacag ggacgatgcc gtaccgttct ctccattcac aacctcggct atcaggggcg
     1201 cttccatttc ggcttcgttt cccatctgct gggcatcgag agccgctatt atcatccgga
     1261 caatatcgaa tatcacggag acgcgaattt tttaaagggc ggcatcgttt acgcggacaa
     1321 gctggttacg gtcagtccga cttacgcccg ggagatcctg acggcctatt acggcgaggg
     1381 gctggagggc attctggcca ctcgggcgga ggacctgacc ggcattctca acgggattga
     1441 caccaacgcc ttcaatccgg cggcggaccc gttcatcccc tatccgtatg acgcctcgtc
     1501 tctttccgca aagcagaaaa acaaggaggc cctcatagcc gagctcggcc tcaaccttcc
     1561 tttcgacgcc cccatcatcg gcatggtaac aaggctgacg aagcagaagg gaattgacct
     1621 ggtccagcgg gtcttcgacg aaatcatcgc ggcgggggca ggtttcgcgc tgatcgggac
     1681 gggcgatcac gcctatgagc actttttccg cggggccgcg caccggacgg gcggcagggc
     1741 cgtcggcgtg acgcagttca atgatccgct ggcgcgccgc atctacgcgg gatgcgattt
     1801 tttcctgatg ccctcgcagt ttgaaccctg cggcctttcg cagatgatcg cgctgcgcta
     1861 cgggacgctg ccgattgtcc gggcgacggg cgggcttcgg gacacggttt ttccctacaa
     1921 tgaatacacg gacgaaggca acggcttcac ttttgaaaac tacaatgcgc acgacatgct
     1981 tcatacgatt cgctacgcac ttggggtcta tgagaacaag gagcgtctgg actcgcttcg
     2041 cctgcgggcg atgggccagg acttgagttt tcaaaaaagc gcgcttgctt acgcgaagtt
     2101 atatgcggat gcggccggaa aatgagccgc ggattttcca ttgacaggcg ccggggcgta
     2161 aaataaaaga attccccatt ccggaggaag cgggccatgc ttccgattca atgattaaag
     2221 gcgaacaggt gaaaaacagg attgcgaaaa taaaaggaat tattgagggg aaactgaaac
     2281 ggcactacgg ccgcacgctg gagaacgcct cggacgagga catgtttcag gtcacggcga
     2341 tgagcgtccg cgacatcatg ctggagcggg gggtcctggc caacgaaacg gttgaaaaga
     2401 aagggcagaa aacgctctgc tatctttcgg cggagttttt aatgggtaga gccctggtga
     2461 acaatctcat caatctcggc ctgcttacgg agtaccgggc cgttatggct gaaatcggct
     2521 acccctttga aaagctcgaa gagcaggaaa tggaagcggc gctgggcaac gggggcttgg
     2581 ggaggctcgc ggcctgcttt ctggattctc tttccacgct gaatcttccg gtgaccggct
     2641 acggcatccg ctacgaatac ggcatgttcc gccagcacat cgttgatggg cggcagacgg
     2701 aagttccgga cgactggacg gcaaagggcg acatctggga aatcgaacgc cgcgaggaac
     2761 aggtggaggt ccactttgac ggcacggtgg aagaggtgtg gactgacagc ggaatgacct
     2821 gcgagcacag ggattttcag accgtactcg ccgtgcccca tgacatgccg gtcatcggct
     2881 atgaaagcca cacgcctgcg acgctccggc tctggcgtgc ggaatgtccg tccggatttg
     2941 atcttcactc cttcaacaag ggcgattttg tctgcatggt gcgccagaaa gagctggccg
     3001 agtcgatttc caaggtgctg tatccccagg acaaccacat gaccgggcgc atgctgcgcc
     3061 tgcggcagta ttattttctg gcttccgcca cgatgcagtg catcgtccgc g
//
LOCUS       DAOSIM010000237         3103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_27338_length_3103_cov_7.223753, whole genome shotgun sequence.
ACCESSION   DAOSIM010000237 DAOSIM010000000
VERSION     DAOSIM010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27338_length_3103_cov_7.223753"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PLR47_09150"
     CDS             complement(<1..471)
                     /locus_tag="PLR47_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HOG13154.1"
                     /translation="MVCFRKRQAGKALWGGLLALALVLIGLPCLCLADAGVEDRLAIC
                     AGIQEDAARLQCFDELAGKVKTAPHVTHAEDKSAPLGRVDAPEPVRLSVMTKEWDLDT
                     DHRSHSFVIRPHRMNYFLPVAYNSSPNDEANLEYDPSAKAQHNEAKFQISFKAKI"
     gene            653..2554
                     /locus_tag="PLR47_09155"
     CDS             653..2554
                     /locus_tag="PLR47_09155"
                     /inference="COORDINATES: protein motif:HMM:NF016915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13155.1"
                     /translation="MKIMSKTVRILLVIMIVAALGGCALFKLREDVRISKDSCLLFGE
                     VLHPSKQQKPIIVVAYSRSDGIIQVADYAVLSEAGQYELLAQEGSYEIFAFEDVNGSL
                     SYDPGEPGGFYGKPDPVKTQMGGVIVGLDIMLDASGKKPAPTFAGSLAAFSGGKSKPP
                     TSAGFVADLNNPAFSAENGAAGFWAPLEYFKRTGCNIFFLEPYDPKKTPILFVHGAAG
                     SPQDWRCFIQNLDRSRYQAWVFYYPSGARLDTIYFLLQTKLYELHRKYNFENLYIVAH
                     SMGGLVSRAAIMEDSTFSDSIRLFVSISSPWGGEGRAKTGVEKSPAVIPSWKDVEPDS
                     PYIQRIFAKKLDPSIRYYLFFGHKGGGSLFRQNNDNTVTLESMLDPRAQADALKVTGL
                     NEDHVSILCSPEMLRHFQTVLAATEEYKDKTYVRSRGYVQVEHAFDPPGTKIPSQMSL
                     VLAPSGTNQKEKQLKIDPLQFRQETGAIVPGRYDVSLCALGFRTEPDHQALEVKAGQI
                     TEAKFTLKPQGMIANLIYAKTALDDRFWGFYRELPGKAKIRSIRLTGQGISRSLTPDD
                     RMSDREVLATFLASRDYAHKNSFAFFDLPAGAYDLTIEADGCEAWSEKVKAQPGEFVP
                     PAPIRLIVK"
     gene            complement(2562..>3103)
                     /gene="kdsB"
                     /locus_tag="PLR47_09160"
     CDS             complement(2562..>3103)
                     /gene="kdsB"
                     /locus_tag="PLR47_09160"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOG13156.1"
                     /translation="MTSAAHRSGTDRIAEAVRSLNLSADAIVVNIQGDQPIFEPMQVD
                     EVITPLLADPAVGMSTLIYKIIRPEEITHPHAVKVVFDADFNALYFSRSTIPFVRDKN
                     LAADYYKHHGIYAYRLDFLGVFTKLPEGKLEKLEALEQLRALEYGYKIKVVITPHDSV
                     EVDNEQELDRVRRILQERK"
BASE COUNT          692 a          897 c          849 g          665 t
ORIGIN      
        1 gatcttggcc ttgaagctga tctggaattt ggcttcatta tgctgcgctt tggccgaggg
       61 gtcgtattcc agattcgcct catcattagg cgacgaatta taggcgaccg gcagaaaata
      121 gttcatccgg tgcggtctga tcacaaaaga atgactgcgg tggtccgtgt ccagatccca
      181 ctctttggtc atgacggaga gccggaccgg ctccggagcg tccacccggc ctaaaggcgc
      241 ggatttgtct tccgcatgcg taacgtgagg cgctgttttc acttttcccg caagctcatc
      301 gaagcactgc aaacgcgctg catcctcctg gatccccgcg caaatagcca gacgatcttc
      361 cactccggca tcagcaaggc aaaggcaagg caaaccgatc aaaacaaggg ccagtgccag
      421 gaggccgccc caaagcgcct tgcccgcctg ccgctttctg aaacacacca ttttctcctc
      481 ctttccaccg gacggcgacg gtccggcgcg ccgcgaccgc aagtccttcc tttgacattg
      541 caacggcccg cggcttctga tagacttccg gcgaatgttt aaacagatga tctggaataa
      601 tgcaacgggc tgctaacacg ggctcgtggg ccgctgcatc ggagccggaa aaatgaaaat
      661 catgtcaaaa acagtccgta tccttcttgt gatcatgatc gtcgcggcgc ttggcggctg
      721 cgcgcttttc aagctgcgcg aggacgtccg gatttccaag gattcctgcc tgcttttcgg
      781 cgaagtcctc catccgtcca aacagcaaaa acccattatc gtggtcgcct acagccggtc
      841 cgacggaatc atccaggtcg ccgattatgc cgtattgtcg gaggccgggc agtacgaatt
      901 gctggcccaa gaggggagct atgaaatttt tgcctttgag gacgtcaacg gatccctctc
      961 ctatgacccg ggtgaaccgg gcggcttcta cgggaagccc gatccggtga aaacgcagat
     1021 gggcggtgtc atcgtgggat tggacataat gctcgatgct tccggcaaaa aaccggcgcc
     1081 gacgtttgcc ggcagtctgg cagccttttc cggcggcaaa agcaagccgc cgacttccgc
     1141 cggatttgtg gcagacctca acaacccggc cttttccgcc gaaaacggag ccgccggatt
     1201 ttgggcgccg ctggaatact tcaagcggac cggctgcaat atttttttcc ttgagcccta
     1261 cgatcccaaa aaaacgccga ttcttttcgt gcatggagcc gccggctccc cgcaggactg
     1321 gcgctgtttc atccagaatc ttgaccgctc ccgttatcag gcctgggtct tttattaccc
     1381 ctccggcgcc cggctggaca cgatctattt tctcctgcag accaaactct acgaactgca
     1441 tcggaaatac aacttcgaaa acctgtatat cgtcgcgcac agcatgggag gactcgtttc
     1501 ccgcgccgcc attatggaag acagcacctt ctccgattcc atccgtcttt ttgtttcgat
     1561 ttcctcgccc tggggaggag aggggcgggc aaaaaccggc gtggaaaaat cacccgccgt
     1621 cattccaagc tggaaggacg ttgaaccgga cagcccttac atccagagga tctttgccaa
     1681 gaaactggat ccgtcgatcc gctactatct gtttttcggc cataagggag gcggcagtct
     1741 tttccggcag aacaacgaca ataccgttac gctggagagc atgctggatc cgcgggcgca
     1801 ggcggacgcg ctgaaagtga ccggcctcaa tgaagatcat gtcagcattc tgtgcagtcc
     1861 ggaaatgctt cggcacttcc agacggtgct tgccgctacg gaggaataca aggacaaaac
     1921 ctacgtccgc tccaggggat acgtgcaggt ggagcatgcc ttcgatccgc ccggcacgaa
     1981 aattccttcg cagatgtcgc tggtgctggc gccgtccgga acgaatcaaa aggaaaagca
     2041 gctcaaaatc gatccgcttc aattccggca ggagacgggg gccatcgtgc ccggccgata
     2101 tgatgtcagc ctctgcgcgc tggggttcag gacggaaccg gatcaccagg cccttgaggt
     2161 caaggccgga cagataacgg aagccaaatt tacgctgaaa ccgcagggaa tgatcgccaa
     2221 cctcatctac gccaaaacgg cgctggatga ccgcttctgg ggattttaca gagaattgcc
     2281 gggaaaagcg aaaatccgct ccattcggct gaccggccag ggcatctccc gatcattgac
     2341 gccggacgac cgaatgagcg accgggaagt gctggccacc tttctggctt cccgggacta
     2401 cgcccacaaa aactcttttg ctttttttga tttgcccgcg ggagcgtatg atttaaccat
     2461 tgaagccgac ggctgcgaag cctggtcgga aaaagtaaaa gcgcagcccg gagaatttgt
     2521 tcccccagcg cccattcggc ttattgtgaa ataaaaagga tttattttct ttcctgcagg
     2581 attcggcgaa cccggtcgag ctcctgctca ttgtccactt ccacggaatc gtgcggcgtg
     2641 atcaccacct tgattttgta gccgtattcc agcgcccgaa gctgttccag ggcttcgagc
     2701 ttttccagtt tcccctcggg cagtttggtg aatacaccaa ggaaatcaag tcgatacgcg
     2761 taaatcccgt gatgcttgta gtagtccgcc gcaagatttt tgtcgcgcac aaagggaatc
     2821 gtcgagcgcg aaaaatacag cgcgttgaag tcggcgtcaa aaaccacctt gaccgcgtgc
     2881 gggtgggtga tttcctccgg ccgaatgatt ttgtaaatca gcgtggacat gccgacagcg
     2941 gggtccgcca gcaggggcgt gatcacttca tccacctgca tcggctcgaa aatcggctgg
     3001 tcgccctgaa tgttgacgac aatcgcatcg gcggaaagat tcaaagaccg gacggcttcg
     3061 gcaatgcggt ccgtgccgga gcggtgcgcc gcagacgtca tga
//
LOCUS       DAOSIM010000238         3090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_27497_length_3090_cov_9.070181, whole genome shotgun sequence.
ACCESSION   DAOSIM010000238 DAOSIM010000000
VERSION     DAOSIM010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27497_length_3090_cov_9.070181"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1858)
                     /gene="carB"
                     /locus_tag="PLR47_09165"
     CDS             complement(<1..1858)
                     /gene="carB"
                     /locus_tag="PLR47_09165"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOG13157.1"
                     /translation="MPKRTDIQKILLIGSGPIIISQACEFDYSGTQAVKALREEGYEV
                     VLINSNPATIMTDPEMADRTYVEPITPEAVEKIIARERPDALLPTLGGQTGLNTAVAL
                     AESGVLARYGVEMIGASLPVIHKAEDREKFRHAMERIGLSMPKSGFARTMEDVRRIAE
                     NIGFPIIIRAAFTLGGTGSGVAYNWEELNEIAKAGLDASMISEVMVEQSVLGWKEYEL
                     EVMRDRADNVVIICSIENFDAMGVHTGESITVAPAQTLTDREYQKMRDAAIAIMREIG
                     VETGGSNVQFAVHPNTGEMIVVEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     IPNDITRETMASFEPAIDYVVTKIPRWTFEKFPETEDFLTTSMKSVGETMAIGRTFKE
                     SLQKAIRSLETGRFGFGQALPDEKQARDELLKSKLIKPNSLRLFYIAEALKSGKTVDD
                     VYVWTKVDPWFLHQIQDILEAEKELAGAEPSAKLLRDAKAMGFSDRALGALWNKTEFE
                     ITCAAEFEAYTPYYYSTYEQESETRPSAKKKVAIIGGGPNRIGQGIEFDYCCVHASFA
                     LREEGIESIMINSNPETVSTDYDTSDKLFFEPVTLEDVLHILEAEKPDGVIVQ"
     assembly_gap    1866..1875
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1876..2817)
                     /gene="carA"
                     /locus_tag="PLR47_09170"
     CDS             complement(<1876..2817)
                     /gene="carA"
                     /locus_tag="PLR47_09170"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOG13158.1"
                     /translation="MSLFKKNRRALLVLEDGSVFEGSAFAGEGEALGEVVFNTGMTGY
                     QEIVTDPSYKGQIVAMTYPLIGSTGVNDEDMESAGLHLEGFIVREYQSHPSNWRMTKS
                     LKAFLEDAGKIGMEGLDTRALTRRLRLSGSMKGIISTLSRDIPSLLTKVQNAPGLVGR
                     DLVRGVSCSKPYRWEKGAPETVSLPVGKSPGKLRVVVLDCGVKYNILRLLELQGCEVI
                     VLPAGATDSQILSCSPDGVLFSNGPGDPAVLPYIVEAARGVIGKTPVFGICLGHQILG
                     QAVGGRTEKLRFGHHGINQPVKNLETGRVEISSQNHGF"
     gene            complement(2804..>3090)
                     /gene="pyrG"
                     /locus_tag="PLR47_09175"
     CDS             complement(2804..>3090)
                     /gene="pyrG"
                     /locus_tag="PLR47_09175"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416741.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HOG13159.1"
                     /translation="ISERHRHRYEFNNDFKKTLTEKGLRITGASPDGALAEIVEIADH
                     PWFLGCQFHPEFKSRPTSPHPLFSKFIETALKRPGMRQRSQGDRQPHVPL"
BASE COUNT          587 a          888 c          898 g          707 t
ORIGIN      
        1 actgcacgat cacgccgtcg ggcttttccg cctcaagaat gtgcaggacg tcttccagcg
       61 tgaccggctc gaaaaacagt ttatcggagg tgtcgtagtc ggtgctgact gtctccgggt
      121 tggagttgat catgatgctt tcgatgcctt cttcacgcaa ggcaaacgag gcatgaacgc
      181 agcagtagtc aaactcaatg ccctggccga tgcggttggg cccgccgccg atgatggcca
      241 ctttcttttt ggccgagggc cgtgtttcgc tttcctgttc gtaggtggaa tagtaataag
      301 gcgtgtaggc ttcaaactcc gccgcgcagg tgatttcgaa ctccgtttta ttccagagcg
      361 cccccagcgc gcggtcggaa aaacccatgg ccttggcgtc gcgcaaaagt ttcgccgagg
      421 gttcggcccc ggccaactct ttttccgcct ccagaatatc ctgaatctga tgcaaaaacc
      481 agggatcgac tttcgtccaa acgtaaacgt catcgaccgt cttgccgctt ttcagcgctt
      541 cggcgatgta aaagagacgc agggaattgg gtttgataag cttgcttttc aaaagttcgt
      601 ctctggcctg cttctcatcg ggcagcgcct ggccgaagcc gaagcggccg gtttccagcg
      661 aacggatcgc cttttgcagc gattctttaa atgtccggcc gatcgccatc gtttcgccca
      721 ccgacttcat cgacgtcgtg aggaaatctt ccgtctcggg aaatttctca aacgtccagc
      781 ggggaatttt tgtcaccaca tagtcaatcg caggttcaaa ggacgccatg gtttcgcggg
      841 tgatgtcgtt gggaatttca tcgagcgtgt agcccaccgc aagctttgcc gcgatcttgg
      901 caatcgggaa ccccgtggct ttggaggcca gcgccgacga gcgcgaaacg cggggattca
      961 tctcgacgac aatcatctct ccggtgttcg gatgaacggc aaactgcaca ttggatccgc
     1021 ccgtttccac gccgatttcc cgcatgatgg caatcgcggc atcccgcatt ttttgatact
     1081 cgcggtcggt aagcgtctgc gcgggggcga cggtgatcga ttcgccggtg tgtacgccca
     1141 tcgcgtcgaa gttttcgatc gaacagataa tgacgacgtt gtcggcgcgg tcgcgcatca
     1201 cttccagctc gtattctttc cagcccagca ccgactgctc gaccatcact tcggagatca
     1261 tgctggcgtc gaggcccgcc ttggcgattt cgtttagctc ttcccagttg taggctacgc
     1321 cgctgccggt gccgcccagg gtgaaggcgg cgcggatgat aatcggaaag ccgatgtttt
     1381 ccgcgatccg ccggacgtct tccatggtgc gggcaaatcc gcttttaggc atggaaaggc
     1441 cgatgcgctc catcgcatgg cggaactttt cacggtcttc ggccttgtgg atgaccggca
     1501 gagaagcgcc gatcatttcc acgccgtacc gggcgaggac gcccgattcg gccagggcca
     1561 cggcggtatt gaggccggtc tgtccgccca gcgtgggcaa aagcgcgtcc ggccgctcgc
     1621 gcgcgatgat tttttccacg gcctcgggcg tgatcggctc cacgtaggtg cggtcggcca
     1681 tttccggatc ggtcatgatg gttgccggat tggagttgat gaggacaact tcatagcctt
     1741 cttcccgcag ggctttcacg gcctgcgtgc cggaatagtc aaactcacag gcctggctga
     1801 tgatgatcgg cccggacccg atgagcagta ttttttgaat gtcggttcgt ttcggcacgg
     1861 gttacnnnnn nnnnnaaaac cgtggttctg ggacgaaatt tccacccggc cggtttccag
     1921 atttttcacc ggctggttga tgccgtgatg gccgaacctg agtttttcgg tcctgccgcc
     1981 cacggcctgg ccgagaatct gatgccccag acagatgccg aagaccggcg ttttcccgat
     2041 cacgccgcgt gcggcctcca caatgtacgg caggacggcc ggatctcccg gcccgttgga
     2101 gaaaagaacg ccgtcgggtg agcaggacag aatctgggaa tccgtggccc cggccgggag
     2161 aacgatcact tcgcagcctt gcaattccag aagccgcaga atattatatt tgacgccgca
     2221 gtccagcacg accactcgga gctttcccgg agatttcccg acaggaagac tgacggtttc
     2281 cggagcgcct ttttcccagc gatagggctt ggagcaggaa acgccgcgca ccaggtcccg
     2341 tccgaccagc ccgggggcgt tttgcacttt tgtaagcaga gagggaatgt cccgggatag
     2401 ggtcgaaatt attcctttca ttgaaccgga aaggcgcagg cggcgcgtca gtgcgcgcgt
     2461 gtccagcccc tccatgccga tttttcccgc atcctccaga aaggccttga ggcttttcgt
     2521 catccgccag ttgctggggt gggattgata ttcccggacg atgaagccct ccagatgaag
     2581 tcccgccgac tccatatcct cgtcattgac gccggtgctg ccgatcagcg gatacgtcat
     2641 ggcgacgatc tggcctttat aggaagggtc ggtgacaatt tcctgatagc cggtcatgcc
     2701 ggtgttgaag acgacttccc ccagggcctc tccctcgccg gcaaaggcgg aaccttcaaa
     2761 aacgcttccg tcttcaagaa cgagcaaagc ccgccgattt tttttaaaga gggacatggg
     2821 gttgcctatc tccttgcgac ctttggcgca tgccgggccg tttcaacgcc gtttcgataa
     2881 atttgctgaa aagcggatgc ggcgacgtcg ggcgggattt gaattccggg tgaaactggc
     2941 acccgagaaa ccacgggtga tcggcaattt cgacgatctc cgccagcgcg ccgtccggcg
     3001 acgcgccggt gatgcgcagt cctttttcgg tcagcgtctt tttaaagtca ttgttgaatt
     3061 catagcggtg gcggtgcctc tccgagattt
//
LOCUS       DAOSIM010000239         3019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_28365_length_3019_cov_6.776316, whole genome shotgun sequence.
ACCESSION   DAOSIM010000239 DAOSIM010000000
VERSION     DAOSIM010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28365_length_3019_cov_6.776316"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..812
                     /locus_tag="PLR47_09180"
     CDS             132..812
                     /locus_tag="PLR47_09180"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOG13160.1"
                     /translation="MAHTEFIERDAPAIAAFAKKAAAGAATPREKAVKLFYAVRDGIY
                     YDPYRIDPRREAFRATTILRQGYGYCVTKAVLLAALMRSQGIACRIHLADVRNHLTTK
                     RLKEMMKTDIFYNHGYNDVWLDGRWLKVTPTFNLSLCEKFKVKPLDWDGQSDAVLHPF
                     DMEGRRHMEYIHDRGSFDDLPFDFLVETFITRYGGSIEEFQRQTAKTGRFEEEAEGEA
                     KKVSGKSV"
     gene            809..2176
                     /locus_tag="PLR47_09185"
     CDS             809..2176
                     /locus_tag="PLR47_09185"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOG13161.1"
                     /translation="MTTEADLSELKAILDERRVSAGQSVRHLHSRDESFHSPSLPEVV
                     VWPRSTEEVSRLVRWACRKKVPVTAWGAGTSLEGNPIPVRGGMVMDFSEMNRVLALLA
                     EDFQADVQPGVVYKELNRQLAREGLFFPPDPGAAATIGGMIGNNASGIRSVRYGATKD
                     YVMRLTVVLPGGDVIHAGNRARKSSSGYNLAALFTGSEGTLGIITEATLKLAGLPVNF
                     MAVRATFPAVRRATDTVYAIMRAGLSPVAMEFLDANVVHALNADRGLTLEENPTLLME
                     FSGYSEQGLSEEMSFVEEICFAKGALSLDRGIGAEERARLWEIRHQTYESVKRCHIGL
                     SPLIMDVAVPLSRYGEMVEFSKQEVRDLTAYLFGHAGDGNIHVHVMDNPQDPKRWARV
                     EEASRSIVMKALEFEGTCTGEHGVGIGKSRFMEREHGASLILMKRLKGLLDPENLLNP
                     GKFFL"
     gene            2217..>3019
                     /locus_tag="PLR47_09190"
     CDS             2217..>3019
                     /locus_tag="PLR47_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13162.1"
                     /translation="MENIIYRGVLTKTAERSFLAGDNGSRHLEDEKIWSGYLTHWSGK
                     KVCARRLPQKDYDTGRPLLILWPDEPALTPPFVEIYFNERLVKYPLSFLGHLAVLADG
                     KVFNFSHWMNENEAMNPEEYFFRPALGEFAPDPASGRDNTENPQRPYYDKFGRLFMRT
                     IHVLRISGLDTRRLSGFFFAELEKIRGTPTDPKEPGKYRDFHILTRSCATIIRDGFHS
                     LGFEKIRGVFPRDLFVSMAYFFLKQHRLPNAQASLHTLRQLQVPEAAPSA"
BASE COUNT          644 a          935 c          874 g          566 t
ORIGIN      
        1 ccgagaacat tgtctcgcac cgctttgggc tggatcaggc ggcggaagct ttccgcgccc
       61 agcttcagga ccccgccgcc gtcaaggtca tgattatgcc tcaagaaagg tgaactatgc
      121 cggaatcctg tttggcgcat acggaattca tcgagcggga cgcccccgca attgccgctt
      181 tcgctaaaaa agccgccgcg ggcgctgcga cgccccggga aaaagccgtc aagctcttct
      241 acgccgtccg cgacggcatc tattacgacc cctaccggat cgatccccgg cgcgaagcgt
      301 tccgggcaac caccatcctt cgccagggat acggatactg cgtgaccaaa gcggttctgc
      361 tggccgcgct gatgcggtcg cagggcattg cctgccgaat ccatctcgcc gatgtccgca
      421 atcacctcac cacaaagcgc ctcaaagaaa tgatgaagac ggacattttt tacaatcacg
      481 gctacaacga cgtttggctg gacggccgct ggctgaaagt gacgccgacc ttcaacctgt
      541 cgctatgtga aaaattcaaa gtcaagccgc tcgactggga cggacaatcc gacgccgtgc
      601 ttcatccctt tgacatggag ggacggcggc acatggaata catccatgac cgcggctcct
      661 ttgacgatct gccctttgac ttcctcgtcg aaaccttcat cacgcgctac ggcggctcga
      721 tcgaggaatt tcagcgtcag acggccaaaa ccggccggtt tgaagaagaa gccgagggcg
      781 aggccaagaa agtatccgga aaatccgtat gaccacagag gctgatctct ccgaactgaa
      841 agccattctg gacgaacgcc gcgtctccgc gggtcaatcc gtgcgccatc ttcattcccg
      901 tgacgaatcc tttcactcgc cttcccttcc ggaggttgtc gtgtggcccc gctccaccga
      961 agaagtcagc cgtctggtcc gctgggcctg ccggaagaaa gtccccgtga cagcctgggg
     1021 cgcgggaacc agcctggagg gaaatcccat tcccgtccgc ggcggcatgg tcatggattt
     1081 cagcgaaatg aaccgcgtcc tggcgctgct cgcggaggac tttcaggcgg atgttcagcc
     1141 cggcgtcgtt tacaaggagc tcaaccggca gctcgcccgc gaaggtctct tctttccgcc
     1201 cgatccgggc gcggcggcca ccatcggcgg catgatcggc aacaacgcca gcggcatccg
     1261 gtcggtgcgc tacggagcaa caaaggatta cgtcatgcgc ctgaccgtcg ttttgccggg
     1321 aggcgatgtc attcacgcgg gcaaccgcgc gcgcaaaagt tcgtcgggct acaatctggc
     1381 cgctctcttt accggatcgg aaggaacgct gggcatcatc acggaagcca cgctgaagct
     1441 tgccggtctg ccggtgaact tcatggccgt ccgggcaacc ttcccggccg tccgccgggc
     1501 gaccgacacg gtgtacgcga tcatgcgcgc gggcctgtcg cccgtcgcca tggaatttct
     1561 ggacgccaat gtcgtgcacg cgctcaacgc cgaccgggga ctgacgctgg aggaaaatcc
     1621 cacgctgctg atggagtttt ccggatacag cgaacagggg ctcagcgaag aaatgagctt
     1681 tgtggaagag atctgctttg ccaaaggcgc gctttcgctc gaccgcggga tcggcgcgga
     1741 ggaaagggcc cgcctctggg aaatccgcca tcagacctac gaatccgtca agcgctgcca
     1801 catcggcctt tccccgctga tcatggacgt ggcggtgccc ctgtcccgct acggcgagat
     1861 ggtggaattc agcaagcagg aagtgcggga cctgacggcc tacctcttcg gccatgccgg
     1921 ggacggcaac attcatgtcc atgtcatgga caatccgcag gatcccaagc gctgggcccg
     1981 cgtggaagaa gccagccgca gcatcgtcat gaaagcgctg gagttcgaag gcacctgcac
     2041 cggcgagcac ggcgtcggca tcggcaaaag ccgcttcatg gagagggagc acggggcaag
     2101 tctgattctc atgaagcggc tgaaaggact gctcgacccg gaaaaccttc tcaatcccgg
     2161 aaaatttttt ctctgatcca aacccgccgg aaaggccgtc aagggagctt cccgccatgg
     2221 aaaacatcat ctacaggggc gtgctgacca aaaccgctga acggtctttc ctggccggag
     2281 acaacggcag ccggcatctg gaagacgaaa aaatctggag cgggtatttg acgcactgga
     2341 gcggaaaaaa ggtatgcgcc cgcaggctgc cgcagaaaga ttatgatacg ggaaggcccc
     2401 tcctcatcct gtggcccgac gagcccgcgc tcacgccgcc cttcgtcgag atctacttca
     2461 atgagcggct cgtgaaatac cccttgtcgt ttctggggca tctggccgtt ttggcagacg
     2521 ggaaagtctt caacttttcg cactggatga acgagaacga ggccatgaat ccggaagaat
     2581 attttttccg gccggcgctg ggcgaattcg ccccggatcc ggcgagcgga cgcgacaaca
     2641 cggagaatcc ccaaaggcct tactacgaca agttcggcag gcttttcatg cggacgattc
     2701 atgtgctgag gatttccggc ctcgacacgc gaaggctatc cggatttttt ttcgccgaac
     2761 ttgaaaaaat ccgcggcacg ccgaccgatc cgaaagagcc cggcaagtac cgggactttc
     2821 acatcctgac ccgaagctgc gccacgatca ttcgggacgg atttcatagc ctgggatttg
     2881 agaagatccg cggcgtcttc ccccgggatc tgttcgtcag catggcttat ttcttcttaa
     2941 aacagcatcg gctgccgaac gcacaggctt cccttcatac gctccggcag cttcaggttc
     3001 ccgaggccgc ccccagcgc
//
LOCUS       DAOSIM010000240         3008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_28494_length_3008_cov_8.365730, whole genome shotgun sequence.
ACCESSION   DAOSIM010000240 DAOSIM010000000
VERSION     DAOSIM010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28494_length_3008_cov_8.365730"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..504
                     /locus_tag="PLR47_09195"
     CDS             259..504
                     /locus_tag="PLR47_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13163.1"
                     /translation="MKKVAGYVVSLLLLFAVLGCASYYKVVDPVSKNVYYTQSIDNRG
                     SGVIQFKDQVSKNKVTLPQSEIMEITEDQFLAGTRGQ"
     gene            594..1574
                     /locus_tag="PLR47_09200"
     CDS             594..1574
                     /locus_tag="PLR47_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOG13164.1"
                     /translation="MAKEEQSPHGMTLPSYEDLFARNYGIFSEARQERLRRASVLIVG
                     CGGIGGTVAIILARSGVGRFVLVDFDRYEPANMNRQIACFADTLGRNKAQVIGEQIVK
                     INPGAAVEIHDRLLDHVRIASLIPGADVVFPAADDFAFSIMVFRDAQKLGRMALLAVP
                     SGTWANVCLISPRGPSVEDIEGVPRLETYEALKRMFETRKYKLGTYDFPLRADWRIEA
                     YRSFIEDDGRPPQICPSVWICASLAALEVVKHLTGQWKPVVAPRYWMITKNRIRIQRV
                     NGPYVQTLLVWQRRILWRVFQTRLGPGLECLQNLWWPIFCRERKNRQDRI"
     gene            1571..2515
                     /locus_tag="PLR47_09205"
     CDS             1571..2515
                     /locus_tag="PLR47_09205"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOG13165.1"
                     /translation="MKTDDALGYTDLFRRNYGILSTAQQERIRKARILIVGDSGVGET
                     LAVLLSRSGCEHFVLIGRDAYEPADMNRQPCCGADTIGRSKVPVIAEVLKSINSEITV
                     DVFRTLPPPEALDGWMSRADIVIPAVDDLAYSVLLFRAARKNGKTAVLCMSSGAMGWV
                     SVFTPESSTLEDCLGIPDLDYEGLTDVVRTQEFKCAQYHYVTAGGWRVDWYREYFRGE
                     RPLAQLCAVAWLAASLAALEILKVVSGKWPFVCAPRCWSIHKAEVSLARFSRLVKYHR
                     KLGWLIFGGRRGRPLHRWTGLFWSRFFRYWQERQRRAD"
     gene            complement(2629..>3008)
                     /locus_tag="PLR47_09210"
     CDS             complement(2629..>3008)
                     /locus_tag="PLR47_09210"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOG13166.1"
                     /translation="LTGYVHPFAHKIGGMYHLPHGRVIGAVMPPVFEFYNETVSRRLG
                     RLADVIGASKPGMSLHQKAAAFIASIRGFNRAYGIGETIPELREADFPEIAESVHGEC
                     VPYPVPRIMDDGDIFRILKALKG"
BASE COUNT          606 a          827 c          898 g          677 t
ORIGIN      
        1 tttcccgctt tttcctgaac caaatgttca atggggttga acgttatgat gaaaaacttt
       61 gattcgcgat gcaaaagacg cattcgatgc gggatcgatt tggaggagcg cttccggcgg
      121 gaaccgccac gtactcatgc gtttttgcct caagtttgcc ttgacaaaaa cttttggtca
      181 ttttagattt cgcctacaga aaaaattgga ttttccataa gcaacaggca attgccgcgg
      241 ggaaaaggag aagagtaaat gaaaaaagtc gcagggtatg tcgtttcgtt gttgttgctg
      301 tttgccgttc tggggtgcgc cagttattac aaggtcgtcg atccggtttc caagaatgtc
      361 tattacacgc agagcatcga caacaggggc agcggcgtca tccagtttaa agatcaggtg
      421 tccaagaaca aagtgacgct gccgcagtcc gagatcatgg aaatcacgga agatcagttc
      481 ctggccggca cgcgcggtca ataacggcgc ggcatttttg tccggagtgt cccggcagaa
      541 cgcgcggcga agcttttttc cacaacgctt gaattttatc cgtcagggga atcatggcca
      601 aggaagaaca gtctcctcat ggaatgaccc tgccgtccta cgaagatctg tttgccagaa
      661 attacggaat tttttccgaa gcccggcagg agaggctccg ccgggcttcc gttcttatcg
      721 tgggatgcgg aggaatcggc ggcactgtcg cgatcatcct tgcccgctcg ggggtgggcc
      781 gtttcgtgct cgtggatttt gaccgctacg aaccggccaa catgaaccgg cagattgcct
      841 gttttgccga cacgctcggc cgcaacaagg cgcaagtgat cggagagcag atcgtaaaaa
      901 tcaatcccgg tgccgccgtc gagattcatg accggcttct ggaccatgtc cggattgctt
      961 ccctgattcc cggggctgac gttgttttcc cggccgccga tgatttcgcc ttcagcatca
     1021 tggtcttccg ggatgcgcag aagcttggcc ggatggcgct gctggcagtg ccctccggaa
     1081 cctgggcgaa cgtctgcctg atttccccgc gcggcccatc ggttgaagat atcgaaggcg
     1141 ttccccggct cgaaacctac gaagcgctga agcggatgtt tgaaacccgg aagtataagc
     1201 tgggcaccta cgattttcct cttcgggcgg attggcgaat tgaggcctac aggtcgttta
     1261 tcgaggacga cggtcgtccc cctcagattt gcccctccgt ctggatttgc gcatccctcg
     1321 cggccctgga ggtcgtcaaa cacctgaccg gccagtggaa acccgtcgtc gctccgaggt
     1381 actggatgat cacgaaaaac cgcatccgga tccagcgggt caatggcccg tatgtccaga
     1441 cgctgcttgt ttggcagaga aggatcctct ggcgcgtttt tcaaacgcgg ctcggtccgg
     1501 gactggagtg cttgcaaaac ctgtggtggc cgattttctg ccgcgaaaga aagaaccgcc
     1561 aggatcgcat atgaagacgg acgatgctct cggctacacg gatcttttcc ggagaaatta
     1621 cggtatactc tccacggctc agcaggagcg aatcagaaaa gcacgaatcc tcatcgtcgg
     1681 cgacagcggc gttggagaaa cgctggcggt tcttctgtcc cgctccgggt gcgagcactt
     1741 tgtcctgata ggacgggatg cttacgaacc tgcggacatg aaccgtcaac cctgctgcgg
     1801 cgcggacacc atcggccgga gcaaagttcc ggtgattgcg gaggttctga aatccatcaa
     1861 ttccgaaatc accgttgacg ttttccggac gcttcccccg ccggaggccc tggacggatg
     1921 gatgtcccgc gcggacatcg tcattcccgc cgttgatgat ctggcataca gcgtcctgct
     1981 tttccgcgca gcgagaaaaa acggcaagac cgcggttctg tgcatgtctt ccggcgccat
     2041 gggctgggtc agtgtgttta cgcccgaatc gagcacgctc gaagattgcc tcggcattcc
     2101 cgatcttgac tacgagggat tgacggatgt cgtgcgcacg caggaattca aatgcgccca
     2161 gtaccattat gttacggcag gcgggtggcg ggtggactgg tatcgggagt attttcgggg
     2221 cgagcgtcct ctggcacagc tttgcgccgt ggcgtggctc gcggcaagcc tggccgccct
     2281 ggaaatcctc aaagtcgttt cggggaagtg gcctttcgtc tgcgcgcccc gatgctggtc
     2341 gattcataaa gcggaagtgt cgcttgcccg tttcagccgg ttggtcaagt accatcgaaa
     2401 actgggctgg ctgatctttg gcggccggcg gggcaggccg ctgcatcgct ggacaggtct
     2461 gttctggagc cggtttttcc ggtactggca ggagcgccag cgccgcgcgg attgacgggg
     2521 catatcgccg ggatcgtttg tttgattctt ccgcaccggc cggatggcgc cggagtccgg
     2581 tttccttcaa aaagcggacg cggccgtatt cttttctgaa cggccggctc accccttcaa
     2641 tgcttttaag attctgaaaa tgtccccgtc gtccatgatt ctgggaaccg gataggggac
     2701 gcactccccg tgcaccgatt ccgcgatttc cgggaagtcc gcttcccgaa gctccggaat
     2761 ggtctcgccg atgccgtagg cgcggttaaa gccgcgaatg gacgcaatga aggcggccgc
     2821 tttctgatgc aggctcatgc cgggttttga ggctccgatg acatcggcca ggcgcccgag
     2881 gcggcggctg accgtctcat tgtaaaattc aaaaaccggc ggcatcaccg cgccgatgac
     2941 ccgtccgtga ggcagatgat acatgccgcc gatcttatgg gcgaacgggt ggacatagcc
     3001 ggtcaatg
//
LOCUS       DAOSIM010000241         2981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_28820_length_2981_cov_6.945660, whole genome shotgun sequence.
ACCESSION   DAOSIM010000241 DAOSIM010000000
VERSION     DAOSIM010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28820_length_2981_cov_6.945660"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="PLR47_09215"
     CDS             <1..1213
                     /locus_tag="PLR47_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13167.1"
                     /translation="TEHMFFEGDRIKAVREMILADNLEGRKKALAKLLPMQREDFYGI
                     LKAMEGYGVTIRLLDPPLHEFVPHEEDAQMEMAREMGISIDAVKAKVASLHEFNPMLG
                     HRGCRLGITFPEITEMQARAIFEAACQLVREGVDAKPEVMVPLVGIVKELASQKAIIV
                     ETARKVMEETGVKVDYSVGTMIEVPRAAVTADEIAKEAAFFSFGTNDLTQMTFGYSRD
                     DAGKFLPDYVAQKILPVDPFRVLDRDGVGKLVRLAVEKGRGVNAKLKVGICGEHGGEP
                     SSIEFCHLTGLNYVSCSPFRVPIARLAAAQAALKNAAPAKKAVASVKKTVLAKAKEVI
                     KTAKAVKKAVTVAVKTAKKPAVKKAVAKKTAPKKAAAKKPAAKKTVVKKAAPKKASVK
                     KPAAKKTAKKK"
     gene            complement(1313..1792)
                     /locus_tag="PLR47_09220"
     CDS             complement(1313..1792)
                     /locus_tag="PLR47_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13168.1"
                     /translation="MITAVDTNIILDVLIPEEPFGESSKALLDCHLSKGKLILSEVVF
                     AELAARFPSEEELRSFLADTRISLVYSDENSLYLAGSRWAEYARKSAKNRFSCGKCGH
                     AFEPSCPQCKAALTKRLHVLADFLIGAHALVQADCILSRDLGVYKTYFSDLTVIDSV"
     gene            complement(1776..2018)
                     /locus_tag="PLR47_09225"
     CDS             complement(1776..2018)
                     /locus_tag="PLR47_09225"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOG13169.1"
                     /translation="MMITARVTSKGQVTIPKEVRERLGVREGEDIGFEEREGLFVVSK
                     VVTVSPFDKWVGKLKHLKGKRSDDLVREARGHDHSR"
     gene            complement(2165..2578)
                     /locus_tag="PLR47_09230"
     CDS             complement(2165..2578)
                     /locus_tag="PLR47_09230"
                     /inference="COORDINATES: protein motif:HMM:NF024060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HOG13170.1"
                     /translation="MKEKIICFSLGLSSGDTEKGKASFNAANDAACELVVAPVTGDML
                     DMKVGDALGNVISGSKPDDLIPVLPDQFPYRVVVVAALEREQVFSVMRSFKAVLPDPQ
                     SLIFAVITETALGWTFRDYAAHLAQEHEEMKSRNR"
BASE COUNT          759 a          852 c          758 g          612 t
ORIGIN      
        1 caccgaacac atgttctttg aaggcgaccg catcaaggcc gtgcgcgaaa tgattctggc
       61 cgacaacctg gaaggccgca aaaaggcgct ggccaaactg ctccccatgc agcgcgagga
      121 cttctacggc attctcaaag ccatggaagg atacggcgtc accatccgcc tgctcgaccc
      181 gcctctgcat gaattcgttc cgcacgaaga agacgctcaa atggaaatgg cccgcgaaat
      241 gggcatctcc atcgacgccg tcaaagccaa agtggcgtcg ctgcatgaat tcaacccgat
      301 gctgggacac cgcggctgcc gcctgggcat caccttccct gaaattacgg aaatgcaggc
      361 ccgggccatc tttgaggcgg cctgccagct cgtccgcgaa ggcgtggacg ccaagcccga
      421 agtcatggtc ccgctggtcg gcatcgtgaa ggagctcgcc tcgcagaaag ccatcatcgt
      481 cgagaccgcc cggaaggtca tggaagaaac gggcgtgaaa gtcgattact ccgtgggcac
      541 gatgatcgaa gtgccccgcg cggccgttac ggccgatgaa atagcgaaag aagccgcatt
      601 tttctccttc ggcaccaacg acctcacgca gatgaccttc ggctacagcc gcgacgacgc
      661 gggcaagttc ctgcccgact atgtcgccca gaaaatcctg cccgtcgatc cgttccgtgt
      721 gctggaccgc gacggcgtcg gcaaactcgt ccggttggcc gtggagaaag gccgcggcgt
      781 caacgccaaa ctgaaagtcg gcatctgcgg cgaacacggc ggtgaaccaa gctcgatcga
      841 gttctgccac ctgaccggcc tcaactacgt gagctgctcg ccgttccggg tccccattgc
      901 gaggctcgcc gcagctcagg cggcgctgaa aaacgccgct ccggcgaaga aagccgttgc
      961 cagtgtcaaa aagacagtcc ttgccaaggc gaaagaagtc atcaaaaccg ccaaggcagt
     1021 gaagaaagcc gtcaccgtgg ctgtgaagac cgccaaaaaa ccggccgtga agaaagccgt
     1081 cgcgaaaaaa acggcaccga agaaagctgc cgcaaagaaa ccggctgcaa agaaaaccgt
     1141 tgtgaaaaaa gcagcaccca aaaaggcctc cgtgaagaaa ccggcagcca aaaaaactgc
     1201 gaagaagaaa taagttctac gcgactcttc attgaacaac agccggggcg gcctgaaccg
     1261 ccccggctgt tttattccgg ccggtgtttc atcctatgga gtggcgaatt gctcataccg
     1321 aatcgataac cgtcaagtcg ctgaaatagg ttttatagac gccaaggtcc cgagaaagaa
     1381 tgcaatcagc ctggacaagg gcgtgggcgc cgatgagaaa atctgccagg acatgaagcc
     1441 gcttcgtcag ggccgccttg cactggggac aactcggctc aaaagcatgg ccgcatttgc
     1501 cgcaggaaaa cctgtttttc gcgcttttcc tcgcgtattc cgcccatctc gaacccgcca
     1561 gatagagcga attctcatcg gaatagacaa ggcttatcct ggtgtcggca agaaacgacc
     1621 taagctcctc ttcagaggga aaccgggcgg ccaattcagc gaaaaccacc tcagagagga
     1681 taagtttccc tttggataaa tggcaatcca gaagcgcctt ggaggattcg ccgaagggtt
     1741 cttcgggaat cagaacatcg aggatgatgt tcgtgtcaac ggctgtgatc atgcccgcgg
     1801 gcctccctca ccagatcgtc gcttcgcttc cctttcagat gcttcagctt acccacccat
     1861 ttgtcaaagg gggataccgt gacaaccttg ctgaccacaa ataggccctc cctctcctca
     1921 aagcctatat cttccccttc gcggaccccc aacctctcgc gaacctcttt gggtatggtc
     1981 acctgccctt tggacgttac tctggcggta atcatcatgc acctctcggt aaggatattt
     2041 ttccttacca tcatggtaag gggaaaagca tcatttgtca acaaccttta gtctccgcat
     2101 ctttttgcgg ctaagaggaa aagagaggaa aaggggacgg ttctatttat ttttctattt
     2161 atttttaacg gtttcgcgac ttcatttctt catgttcctg cgcaaggtgg gccgcataat
     2221 ccctgaacgt ccagccgagg gctgtttcgg tgatgacggc aaagatcagc gactgcgggt
     2281 ccggcaggac cgccttgaag gaccgcatca cggaaaagac ctgttcgcgc tccagcgcgg
     2341 caaccactac gacccgatag ggaaactgat ccggcaaaac cgggattaag tcgtccggct
     2401 tcgaaccgga aatgacgttt cccagcgcat cgccaacttt catgtccagc atgtcgccgg
     2461 tcacgggcgc tacgaccaac tcgcacgccg catcattggc cgcgttgaac gacgccttgc
     2521 ctttttccgt gtcaccggaa gacaatccca ggctgaagca aatgattttc tccttcataa
     2581 cggcatattc ctccaggctg ttccgaaacg tcaaaaaatt ggttttggcg cgtttaaacc
     2641 gcttctgcag gttgaatatg gtacagggat gccggcaatt gcaagcaaaa aacaaaaatc
     2701 ggatccgtct ggattgataa aatccgcctt tacggcggcg atgaaaatcc ccgctgaacc
     2761 cgatcctttt tcgacaaatc gttaagcttc tttaaaattt ctttcaatca acaagtcttt
     2821 tctgcccgga tccttcaaaa gcaattttat gcaaatatca atatatatcg taatgttata
     2881 acaatctttg tcccgctctg caattctggc acgggttttt cattaggtat aaggcaaatg
     2941 aaacaaaaac cctcagggag gaaacagatc atgaagaaga c
//
LOCUS       DAOSIM010000242         2963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_29043_length_2963_cov_7.351444, whole genome shotgun sequence.
ACCESSION   DAOSIM010000242 DAOSIM010000000
VERSION     DAOSIM010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29043_length_2963_cov_7.351444"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..871
                     /locus_tag="PLR47_09235"
     CDS             89..871
                     /locus_tag="PLR47_09235"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG13171.1"
                     /translation="MFFDPPGSHTAEDRHQTPRGRCLDSRKEKLILDLVAPAAGESVL
                     GVGCGNGDDLLLFGRKKCLLTGMDPSQAALDAARSRLGESCELVLGDAADIPFSDNQF
                     DVVTLTGGLQRLANPRKVLAEAVRVSRNRVFVGFLNRHSPAGTGKAARKLFGFPEVSD
                     VRFFSVGEMRTLIGQAMPGARIQWGSVMCLPGPAYDLAPELEDLLPIRKNPLGAYVGM
                     VFAVHCTYRTVQHPVMDSFQLKAKARAAAPEAVRDMLRGGGR"
     gene            868..1878
                     /gene="amrS"
                     /locus_tag="PLR47_09240"
     CDS             868..1878
                     /gene="amrS"
                     /locus_tag="PLR47_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOG13172.1"
                     /translation="MIREAMLYEKLPEDRVQCSVCAHRCKINSGRRGLCGVRENRDGV
                     LYSLVFGTLIAEHVDPIEKKPFFHVYPGSRSYSVATVGCNFSCSFCQNHEISQMPRST
                     LMIMGEEVPPAMIVERAKETDCRTIAYTYTEPTVYLETALEIAKLACREGLKNLFVTN
                     GYMTPETLDSMSPYLAAANVDLKSFQEAFYKQQCGAMLAPVLDSLKKMKDLGIWVEVT
                     TLLIPGLNDSADELKDIAFFIRELGPETPWHVSRFHPQFKMLSTPSTPVSSLRLAGLI
                     GKEAGLLHVYTGNVPGDEGENTFCSNCGKMLIRRYGFKIIDDFLSEHHCPSCGRKLEG
                     LF"
     gene            2061..>2963
                     /locus_tag="PLR47_09245"
     CDS             2061..>2963
                     /locus_tag="PLR47_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG13173.1"
                     /translation="MKLKRLEIAGFKSFRDKVVIDFSRGVNAVVGPNGCGKSNVVDAI
                     RWVMGEQRVKALRGKKMDDVIFNGSEDAAPVSMAEVVMTLSSDGSSFPGVYGELTEVS
                     ISRKNILDGESEYCINRVPCRLLDVKEFFMGTGVGARTYSLIEQGAVANLVEAKPEDR
                     RLFIEDAAGVSKYKSRKETAVRRMEATKQNILRLNDIHKEVKTQLNAITRQAKKAEQY
                     KAVKQRMKETEIALSRQAYAKLSGKNAALQDERAQIAGQADAVGAKMESRESALQEMK
                     AALLEKDEQIGLRQTELYEVKNSIN"
BASE COUNT          660 a          813 c          863 g          627 t
ORIGIN      
        1 ggactgcacg gacagacacc tgaaaaaatg ccaccgggac atgtcggaca tcggcggctg
       61 aactgtttca acgaggggtg aaggttccat gttttttgat ccgccaggct cccatacggc
      121 agaagacagg catcagaccc cgcggggacg gtgtctggat tcgcgaaagg aaaaactgat
      181 tctggacctg gtcgctcccg ccgccgggga atccgtcctg ggcgtggggt gcgggaacgg
      241 agacgacctg ctcctgtttg ggcggaaaaa atgcctgctc acgggaatgg acccgtcgca
      301 agccgctctg gacgcggccc gaagcaggct tggcgaatcg tgcgaactgg ttttggggga
      361 cgcggccgac attccttttt cggacaatca gttcgacgtg gtcaccctga ccggcggcct
      421 gcagcgcttg gcgaatccgc gaaaagtcct tgcggaggcg gtccgggtca gccgcaatcg
      481 cgtctttgtc ggttttttaa accggcattc cccggccggg accggaaagg ctgcaaggaa
      541 gcttttcggt tttcccgaag tgtcggatgt gcgctttttc tcggttggag aaatgcggac
      601 gctcattggc caagccatgc ccggcgcccg gattcagtgg gggtccgtga tgtgccttcc
      661 cggtccggcg tatgaccttg cgccggaact ggaagacctt cttcccataa ggaaaaatcc
      721 gctgggagcg tacgtcggga tggtttttgc cgttcactgc acgtatcgga ccgtccagca
      781 tcccgtgatg gattcttttc aactcaaggc aaaagcgcgg gctgccgctc cggaagccgt
      841 tcgggacatg ctgaggggag gcgggagatg atccgcgaag cgatgctcta cgagaaactg
      901 ccggaagacc gcgtgcagtg ctccgtctgc gcgcaccggt gcaaaatcaa ttccggccgg
      961 cgcggcctgt gcggcgtgcg cgaaaaccgg gacggtgttt tgtattccct tgttttcggg
     1021 acgctgattg ccgagcacgt ggaccccatt gaaaaaaagc cctttttcca tgtttatccc
     1081 ggttcccgct cctattctgt ggctaccgtc ggctgcaatt tttcctgctc gttctgccag
     1141 aaccacgaaa tctcccagat gccccgttcc acgctcatga tcatggggga agaagtgccg
     1201 cccgcgatga ttgtcgaacg ggcgaaagaa acggattgcc ggacaatcgc ctatacctac
     1261 acagagccga cggtttacct ggaaaccgca ctggaaatcg cgaagctcgc ctgccgggag
     1321 ggcctgaaaa acctgtttgt caccaacggc tatatgacgc cagaaaccct ggattcgatg
     1381 tcgccttacc tggccgccgc caatgtggat ttgaagtcct ttcaggaagc gttttataaa
     1441 cagcagtgcg gcgcgatgct cgctcccgtt ttggacagcc tcaaaaaaat gaaagacctc
     1501 ggcatctggg tggaggtcac cacgcttctg attcccggtc tgaacgattc cgctgacgaa
     1561 ttaaaggata tcgccttctt catccgggaa ctggggccgg aaaccccctg gcacgtcagc
     1621 cggttccatc cgcagttcaa aatgctgtcc accccttcca cccccgtttc ttccctgcgt
     1681 ctcgccggtt tgatcgggaa agaagccgga ttgcttcatg tttacaccgg caacgtgccg
     1741 ggggatgaag gggaaaacac cttctgctca aactgcggca aaatgttgat ccgtcgctat
     1801 ggctttaaaa taattgatga ttttttgtcg gaacatcatt gcccctcctg cggccgaaag
     1861 ctggaaggtc ttttttaaac cagccccgtg aaaaaaccgc tttttcatcc ctcccattcg
     1921 actccatttt caggtcaaag cactatatat tgtggtgtcg ttttttcaaa aatacatttt
     1981 gttgtatttt cactttacaa aggaacgttc ttttgttagg ctttttcaga atttacaacc
     2041 atttccactg aagatgattt atgaagctga aacgtctgga gatagcaggt ttcaaatctt
     2101 tccgcgacaa ggtcgtgatt gacttttccc gcggcgtcaa cgcggttgtc ggtcccaacg
     2161 gctgcggaaa atcgaatgtg gtcgatgcga tacgctgggt catgggcgaa cagcgggtca
     2221 aggcgctgcg cggcaagaag atggacgacg tcatcttcaa cggcagcgag gacgccgccc
     2281 ccgtgagcat ggcggaagtc gtgatgacgc tgtcgagcga cggatcgtcc tttcccggcg
     2341 tttacggcga attgacggaa gtgtccattt cgcgcaaaaa cattctggac ggggagagcg
     2401 aatactgcat caaccgcgtg ccctgccgcc tgctggacgt aaaagagttc ttcatgggga
     2461 cgggcgtggg ggccaggacc tattcgctca tcgagcaggg agccgtggcc aacctggtgg
     2521 aagccaagcc ggaagaccgc cgcctcttca tcgaagatgc ggcaggcgtg tccaagtata
     2581 aaagccgcaa ggaaaccgcc gttcgcagaa tggaggccac caagcagaac attctgcgcc
     2641 tcaacgacat tcacaaggaa gtcaaaaccc agctcaacgc cattacccgc caggccaaaa
     2701 aagcggaaca gtacaaggcc gtgaagcagc gcatgaagga aaccgagatt gcgctgtcga
     2761 ggcaggcgta cgcaaagctt tccggaaaga acgccgcgct gcaggacgag cgggcgcaaa
     2821 tcgccgggca ggcggacgcg gtcggggcga agatggaatc cagggaatcc gcgctgcagg
     2881 agatgaaggc ggccctcctg gagaaggatg agcagatcgg ccttcgccag actgagcttt
     2941 acgaggtgaa gaattccatc aac
//
LOCUS       DAOSIM010000243         2959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_29096_length_2959_cov_6.018939, whole genome shotgun sequence.
ACCESSION   DAOSIM010000243 DAOSIM010000000
VERSION     DAOSIM010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29096_length_2959_cov_6.018939"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLR47_09250"
     CDS             complement(<1..234)
                     /locus_tag="PLR47_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOG13174.1"
                     /translation="MKKSYILALDQGTTSSRAVIYDREGVVVKIAQNEFSQIYPQAGW
                     VEHDPMEIWGSQRGAISEVMAATGISPEEIAAIG"
     gene            421..1197
                     /locus_tag="PLR47_09255"
     CDS             421..1197
                     /locus_tag="PLR47_09255"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOG13175.1"
                     /translation="MTTTIFYYTGTGNSLWTARRLASLLEEAQCVSMKRCTDVKIACG
                     AQRIGLVFPVHIWGVPPPVVEFVRRLNVNTSQYLFAMAVNAGQVAATLIQLQSVLGEK
                     QLRLSSGFSIDLPSNYIPWGGAPAPDIQQKKFDAALAKLNRIAAVVRGEEERPPEKGP
                     FWQNWILSALYRISLPHVAGMDKSFFADEKCSACGICEKICPARNIEIEGEKPVWQHR
                     CEQCFACLQWCPEEAIQYGRGTRNKKRYRHPEISLKDMLA"
     gene            complement(1214..1597)
                     /locus_tag="PLR47_09260"
     CDS             complement(1214..1597)
                     /locus_tag="PLR47_09260"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOG13176.1"
                     /translation="MRQIIVDTDILILHLQGYEDARKALSEAARNSLLCCSAITVGEI
                     HAGMKETEQEATEKLLDSLLVIDVDRNIAALAGDYRRTIKSHQLELDDCLIAATCSIH
                     KATLITGNVKHYPMKDFEKKSVRMT"
     gene            complement(1578..1853)
                     /locus_tag="PLR47_09265"
     CDS             complement(1578..1853)
                     /locus_tag="PLR47_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13177.1"
                     /translation="MNTALKQANFQIPEDLLNELRATVGKGELSKFVSHALKRELQRL
                     RQQTAINETFGIWKSEEHPELAKGINDFVRTLRKSARGKRTNATNHR"
     gene            complement(1946..2443)
                     /gene="lspA"
                     /locus_tag="PLR47_09270"
     CDS             complement(1946..2443)
                     /gene="lspA"
                     /locus_tag="PLR47_09270"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOG13178.1"
                     /translation="MKKNLLMFILGAAVIIALDQITKSAVLARFSLHETLEVIPGFFN
                     LVYVMNPGAAFGFLADASATFRYVFFIGITIAAAGLIVYYLVKSNLRNMLLAVSLTLI
                     FGGAVGNLIDRLRFGAVVDFLDVYVGTAHWPAFNVADSAITCGAILMIWEMLRNRKSK
                     QTSST"
     gene            complement(2620..>2959)
                     /locus_tag="PLR47_09275"
     CDS             complement(2620..>2959)
                     /locus_tag="PLR47_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HOG13179.1"
                     /translation="KSEIAKAVEQARKEKVIGHSLDARISIAAPDKMKDLFAAHLEDL
                     RALLIVSQLQLVDESAIANPYKSEEIKGMAVGVEKARGAKCERCWIYEESVGTHADHP
                     TVCARCLSNL"
BASE COUNT          678 a          782 c          759 g          740 t
ORIGIN      
        1 gccgatcgcc gcgatctctt cgggggagat gccggtggcg gccatcactt cgcttatcgc
       61 gccgcgctgc gagccccata tttccatggg atcatgctct acccagccgg cctggggata
      121 aatctgtgag aattcgtttt gagcgatttt taccacgacg ccttcccggt cataaatcac
      181 cgcccgcgaa ctggtcgtgc cctggtcaag agctaaaatg taggatttct tcatcggcca
      241 ctcctgaaca aaatcaatat tgtcctgcac tatgacataa taccgaggtt tttgccaaat
      301 aaaaaaggcg cgcccgggcg ccatttggaa aatctgcgct ccataaatca tttgacagga
      361 gaagtatcgt gaggatattc tcccacaaaa caaagacctc atcaccggac gtgcaggacc
      421 atgaccacaa caatctttta ctataccggc accggcaact ccctgtggac cgcccgaaga
      481 ctggcttcac ttttggaaga ggcacaatgt gtttccatga agcgttgcac ggatgtaaaa
      541 attgcctgcg gcgcccagcg gatcggactg gttttcccgg tgcatatctg gggcgtgccg
      601 ccgcccgtcg tcgaatttgt caggcggctg aatgtcaata cgtcccaata cctgtttgcg
      661 atggccgtca acgccggcca ggtcgccgcc acgctgattc agttgcagag cgttttaggg
      721 gagaagcagc ttcgtctctc gtccggattt tccatcgatc tgcccagcaa ttatattccc
      781 tggggcggag ccccggctcc cgacattcag caaaaaaaat ttgacgcggc gcttgctaaa
      841 ctgaaccgga ttgcggcggt ggtccgggga gaggaagaac gtccgccgga gaaggggccc
      901 ttctggcaga attggatttt gtccgcgctc taccgaattt cccttccgca tgtggcaggg
      961 atggataaat ctttttttgc cgatgaaaaa tgcagcgcct gcgggatttg tgaaaaaatc
     1021 tgccccgcgc ggaatattga gatagaaggg gagaaacctg tctggcagca ccgctgcgaa
     1081 cagtgttttg cctgcctcca gtggtgtccg gaagaggcga tccagtacgg caggggcacc
     1141 cggaacaaaa agcgctaccg ccatccggaa atttcattga aggatatgct ggcttaaaaa
     1201 agagacggca gagttatgtc attctgacgg attttttttc aaaatccttc atcggataat
     1261 gtttgacatt gcccgttatg agagtggcct tgtggattga gcacgtggcg gcaatcaggc
     1321 agtcgtccag ctccaattga tgacttttaa tggtgcgtcg gtaatctccc gccagggctg
     1381 caatatttct gtcaacatca atcaccagaa ggctatccag taatttttca gttgcctctt
     1441 gctctgtttc cttcatgccc gcatgtatct caccgacggt aatggcggag caacacaata
     1501 gtgaattccg ggcggcctcg ctgagggcct tgcgggcatc ttcgtagcct tgcagatgaa
     1561 ggatcaggat gtccgtgtca acgatgattt gtcgcattgg tcctttttcc ccgcgccgat
     1621 tttctcaaag tgcgcacaaa atcatttatt ccctttgcca attcagggtg ttcctcgctt
     1681 ttccatatgc cgaaggtctc gtttatagct gtctgctgtc gcagacgctg gagttcgcgc
     1741 ttcagcgcat ggctgacgaa cttgcttaat tctccttttc cgacagtcgc ccgcagttcg
     1801 ttcagcaggt cttcaggaat ttgaaaattg gcctgcttca atgcagtgtt catgagcttt
     1861 gcctccattt ctataactct ttataggtat tcttgtagat atacccgtaa aagtcaacaa
     1921 tgaaatatta ctggatcata actgatcagg ttgatgatgt ttgtttgctt ttccggttgc
     1981 ggagcatttc ccagatcatc aggatggcgc cgcaggtaat ggcggagtcc gccacgttga
     2041 aagcgggcca gtgggccgtc ccgacataga catccagaaa atcaacaact gctccgaatc
     2101 gcagccggtc gatcagattt ccgacggcgc cgccgaaaat caaggtcagg gaaacggcca
     2161 gcagcatgtt tcgcagattg cttttcacga gataataaac aatcaaaccg gcggccgcaa
     2221 tcgtgatgcc gatgaagaat acataacgga aggtcgcgga ggcatcggcc agaaatccga
     2281 aggccgcgcc gggattcatg acatagacca ggttgaaaaa accgggaatg acttccagcg
     2341 tttcatgaag agaaaaacgc gccaggacgg ctgattttgt aatttgatcc agcgcgatga
     2401 tgaccgccgc gcccagaatg aacattaaaa gatttttctt caaaaaaact ccttgttgcc
     2461 tgtatggcta tagtgatggc gggtctaaag gcccgcccta cgtttcactc gtcactcttc
     2521 agttttttac aggaatgcag cgcgggtctt tagacccgca ttaggcggac ctgaaggtcc
     2581 gccctacgtt cacttttccc atcacccagg acccgttctc tacagattcg acaggcagcg
     2641 ggcgcaaacc gtgggatgat ccgcatgggt tccgaccgat tcttcataaa tccagcagcg
     2701 ttcgcatttg gcgccgcggg ccttttccac gccgaccgcc attcccttga tttcctcgct
     2761 tttgtaagga ttggcaatgg cggattcatc caccagctgc agttgcgaaa caatcagaag
     2821 cgcgcgcagg tcttccagat gcgcggcaaa cagatctttc attttgtccg gcgcggcgat
     2881 gctgattcgt gcatccagcg aatgtccgat cactttttcc ttgcgcgcct gttcgacggc
     2941 tttggcgatt tcgcttttg
//
LOCUS       DAOSIM010000244         2941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_29324_length_2941_cov_6.978170, whole genome shotgun sequence.
ACCESSION   DAOSIM010000244 DAOSIM010000000
VERSION     DAOSIM010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29324_length_2941_cov_6.978170"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /gene="dusB"
                     /locus_tag="PLR47_09280"
     CDS             complement(<1..879)
                     /gene="dusB"
                     /locus_tag="PLR47_09280"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOG13180.1"
                     /translation="MSNVISDLQGKALLAPLAGVTNLPFRLVARRLGCSLCFTEMISA
                     NGLIRESAQTLEYLKTSPEDLPLGIQLFGADPLVMARAAVMVAGQNPALIDINMGCPV
                     KKVVKTGSGAVLMKNPLLAGRIIAAVVRAAKLPVTVKIRSGWSRSSINAVEIAKIAED
                     SGSSAIIVHGRTADQGFSGVADWNIIGSVKRAVRIPVIGNGDIREPEDALRMLAQTGC
                     DAVMVGRGALGNPWIFMGIHQLLAGEKDHGRPSWPDRQEVMNQHWEDEVRYCGRHFAD
                     RSFRKHLLWYTKGLAGS"
     gene            complement(1001..1726)
                     /locus_tag="PLR47_09285"
     CDS             complement(1001..1726)
                     /locus_tag="PLR47_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOG13181.1"
                     /translation="MILAAGKGTRMKSDMAKVLHVLEGKPLLHYSLKAAREAGAAKIV
                     VVIGHQAGRVREAFADPDLIFVDQMPQLGTGHAVMQAGSALAGYQGLTVILCGDVPLL
                     KPETICSLIRHHQETKACVTVLTTEPPGPHAYGRIVKNSRGDVLKIVEHRDATENERK
                     ILEINTGIYCVETQFLFAALAQVKNDNQQKEYYLTDIVEIARREDRKVQAFLTEDYVE
                     VMGINTLEELGKAGVYLRTGDKR"
     gene            complement(1765..>2941)
                     /locus_tag="PLR47_09290"
     CDS             complement(1765..>2941)
                     /locus_tag="PLR47_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOG13182.1"
                     /translation="GSSAARQAVKLKEHPEWFISTALIGTNLAVIVASTLATGLLIKY
                     FGAKLGEQIAFLIMLPTLFIIIIIRSIFQHYAESMAIRVAHFIRLSSYVFYPLAYSVA
                     AISRGAVNLSSDPKIRQHSYITKEGLKYILGEKTEGGDILTTEKEMVSRVFDFSELTA
                     EKIMVPISALTALPQTMTIEEAACVVAAKKYMRIPVYADTAYNIVGILHYFDLLDMLL
                     QQQQTAAADSEKATVAACMRADVFYVPETKKAGPLLMDMQRKREHMAVVVDEYGGAVG
                     IVTMEDIGEEIVGSIDEEYNKGEKLYKKTAPGRYLVNGRMKIEDLNQILLTRLPEGNY
                     ETLGGFLIAQAGRIPLRRERLEFGGMIFVVEDSDQKSIREVLVIAPQPLEGPLTHTG"
BASE COUNT          638 a          890 c          742 g          671 t
ORIGIN      
        1 cgaaccggcc aaacccttgg tgtaccagag caaatgtttg cgaaaagacc tgtccgcaaa
       61 atgcctgccg caatagcgaa cttcatcttc ccagtgttga ttcatgacct cctgccgatc
      121 cggccatgag gggcggccat gatccttttc gcccgccaat aattgatgaa tccccatgaa
      181 tatccatgga ttgcccaatg cgcctcttcc gaccatcacc gcgtcgcacc cggtttgcgc
      241 cagcattcga agcgcgtctt caggttcccg aatatcgccg tttccaatca ccggaatacg
      301 gacggcgcgc tttacggagc cgataatgtt ccaatccgca acgccggaaa agccctgatc
      361 ggccgtacgg ccgtgaacga tgattgcgga agaccctgaa tcctctgcaa ttttagctat
      421 ttcaacggcg ttgatggagc ttcggctcca accggaacgg attttaacgg ttaccggcag
      481 ctttgccgcc cgaacaacgg ccgctatgat ccggcccgcg agaaggggat tcttcatcaa
      541 tacggcgccg gagccggttt taacgacctt tttcacggga cagcccatat tgatgtcgat
      601 cagggcggga ttttgcccgg caaccattac ggcggccctg gccatgacaa ggggatcggc
      661 cccgaaaagc tgaatcccca gaggcaggtc ctccggggat gttttcaaat attcaagcgt
      721 ttgcgccgat tcgcgaatca ggccgttggc gctgatcatt tcggtaaagc acagcgaaca
      781 gccgagccgg cgggccacca gccgaaacgg cagattcgta acgccggcca gcggcgcgag
      841 cagcgccttg ccctgaagat cagaaattac attggacacg aatcaaaacc atcccactat
      901 agcgagtcta agcaggctgt tgcccaaaca atatcccttt gagcggccat tgagagggtt
      961 ttgttatctt tcctgtcgcc catcgcctct cgccccttac ctatcgctta tcgcctgttc
     1021 ttaaataaac accggctttc cccagctctt cgagggtatt gattcccatc acctcgacat
     1081 aatcctccgt caggaaagcc tgtaccttgc ggtcctcccg ccgggcgatt tccacgatat
     1141 ccgtcaggta atattctttc tgctgattat cgtttttcac ctgggccagc gcggcgaaca
     1201 aaaactgcgt ctcaacgcaa taaatccccg tattgatttc cagaatcttt cgctcgttct
     1261 ccgtggcgtc ccggtgctcg acaattttca ggacgtcgcc gcgggagttt tttacgatgc
     1321 ggccgtaggc gtgcggcccg ggcggctcgg ttgtcagcac cgtcacgcat gccttggttt
     1381 cctgatgatg acgaatcagc gaacaaatgg tttccggctt caaaagcggc acgtcgccgc
     1441 agaggatgac ggtcaagccc tgatagccgg caagcgcgct cccggcctgc atcacggcgt
     1501 gtcctgttcc cagttgcggc atctgatcca cgaaaatcag atccgggtcg gcaaacgcct
     1561 cgcgcaccct gcccgcctga tgtccgatca ccacaacgat tttcgccgcg ccggcttcgc
     1621 gggccgcttt cagcgaatag tgcagcaggg gcttcccttc cagaacatga agcactttgg
     1681 ccatatccga tttcatgcgg gtccccttcc ccgccgcgag aatcaaagcg cagaactgca
     1741 atttcttttc caacgtcttc ccccttagcc cgtgtgtgtc agcggcccct ccaggggctg
     1801 cggagcaatc accaaaactt cccgaatgga tttctgatcc gagtcctcca cgacaaaaat
     1861 catcccgccg aactccagcc tttcccggcg cagggggatt ctccccgcct gcgcaatcag
     1921 aaaaccgcca agggtttcat agttgccctc gggcaacctt gtcaacagga tctggttcaa
     1981 atcctcgatt ttcattcggc cgttcaccag ataccggccg ggggccgttt ttttgtagag
     2041 cttttccccc ttgttgtact cttcatcgat gctgccgaca atttcctccc cgatgtcctc
     2101 catggtcacg attccgaccg cgcctccgta ttcatccacg accaccgcca tgtgctcgcg
     2161 ttttctctgc atatccatca aaagcggacc ggcctttttc gtttccggaa cataaaaaac
     2221 atccgcccgc atgcaggcgg ccaccgtggc cttttcggaa tcggccgccg ccgtttgctg
     2281 ctgttgcaac aacatgtcca gaagatcgaa ataatgcaaa ataccgacaa tgttataggc
     2341 ggtgtcggcg taaacgggaa tgcgcatata tttttttgcc gcgaccacac aggccgcttc
     2401 ttcaatcgtc atcgtttgcg gcagagcggt caacgctgaa atcggcacca tgatcttttc
     2461 cgccgtgagt tcggaaaaat caaagacgcg gctgaccatt tccttttccg ttgtcagaat
     2521 gtcgccgccc tccgtctttt cccccagaat atactttaac ccttctttgg taatatagga
     2581 gtgctgtctg attttcggat cggaagaaag attcaccgcg ccccgggaga tcgccgcgac
     2641 cgagtaggcc agcggataaa acacatagga ggaaagacgg atgaaatggg ccacccggat
     2701 ggccatggac tccgcatagt gctggaagat gctgcggata ataatgatga taaacagggt
     2761 gggcagcatg atcaggaagg cgatttgctc gcctagtttc gcgccgaaat atttgatcag
     2821 aagcccggtg gccagcgtgg acgcgacaat aacggccaga ttggtgccga tgagcgccgt
     2881 ggagataaac cattcgggat gttccttcaa tttgaccgcc tggcgggcgg cggacgatcc
     2941 c
//
LOCUS       DAOSIM010000245         2930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_29434_length_2930_cov_7.016696, whole genome shotgun sequence.
ACCESSION   DAOSIM010000245 DAOSIM010000000
VERSION     DAOSIM010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29434_length_2930_cov_7.016696"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..803)
                     /locus_tag="PLR47_09295"
     CDS             complement(261..803)
                     /locus_tag="PLR47_09295"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOG13183.1"
                     /translation="MRKMQLGKAFTLIESGPVVLITTHDGKKNNIMTISWTMVVDFTP
                     VFAITTGPWNYSFAALQKTKECVMAVPTVDLLDRVVGAGTCSGADTDKFEKFLLTPVK
                     GKHVQAPLIKECLANIECKVVDIVRKHNIVVLEAVAAYFDPSRKERRTLHAVGDGTFI
                     VDGRKLDRREMMRSKIPAGL"
     gene            complement(886..1608)
                     /locus_tag="PLR47_09300"
     CDS             complement(886..1608)
                     /locus_tag="PLR47_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG13184.1"
                     /translation="MKQWTTKGGQVVRRLSHGMYNCFLVSLNGRHLLVDACRKGSWKK
                     LSRELDELGVNGGSLAGLVLTHTHYDHAENAAAIKDRYNPKIIVHKKEADYVRRGENP
                     VIHGTLPLTGWISDRLSHWYLSQFFRYRPVDGDLTVAERYDLKPLGFQGYLLSTPGHS
                     PGSLSVILENEIAIVGDALFGVFKGSAIPPVAADEKRMVASWEKLLKTGCSLYLPAHG
                     SERGREILERQHEKYKRKYDRP"
     gene            1978..2481
                     /locus_tag="PLR47_09305"
     CDS             1978..2481
                     /locus_tag="PLR47_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HOG13185.1"
                     /translation="MIEIFINRSSGRLQAAPIRITLKSHRSRHAAAFRQTLFFKIAGL
                     PVSGEVCAGFPSPAEEELRGHISFDEYLVAHPESSFLLSVTGDSMAGAGIMDKDWVII
                     EKNRRPKNGDIILAEVDGAWTIKYYRKKGTRITLETAGAGDPPMVPREELRIAGVVTA
                     LVRKYHR"
     gene            2640..>2930
                     /locus_tag="PLR47_09310"
     CDS             2640..>2930
                     /locus_tag="PLR47_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOG13186.1"
                     /translation="MSDLIFSLVSWPRAIVHIDGDAFFTSCEEAVHPELRGKPLITGG
                     ERGIVACASYAAKRLGVKRGVPLSDARKMCPDLIILPSDYETYSLFSRRMFAV"
BASE COUNT          612 a          861 c          781 g          676 t
ORIGIN      
        1 ccttgacttt ttctcccagg caccagactt cccggccgtc attcaagctc tccaggtact
       61 gctcaacggt cttgatcatt tttattttcc cttattcgtt gtgagagaaa gcttaaaaaa
      121 tatttttaat gagcaaaatg cctgttcctc catgctcctt tcaatgaagc atgtcaaaat
      181 agtaatgcaa ccgggtggac cagcagcagg catcctccat acaggttcgg aaaccggcgg
      241 accccctgga tgtgctgtca tcagagaccg gccggaattt ttgagcgcat catttccctg
      301 cggtccaact tgcgcccatc cacgataaaa gttccatcgc cgacggcgtg aagcgttcgc
      361 ctctcctttc gtgaaggatc aaagtacgcc gcaacggctt cgagaacgac gatattgtgc
      421 ttccggacaa tgtccacgac tttgcattcg atattcgcca gacattcttt tatcaggggc
      481 gcctggacat gctttccctt cacgggggtc agcaggaatt tttcaaattt gtccgtgtcg
      541 gcgccggaac aggttcccgc gccgacgacc cgatccagca gatcaaccgt gggaacggcc
      601 ataacgcact ccttcgtttt ttgaagagcg gcgaaggaat agttccaggg cccggtggtg
      661 atggcaaata ccggcgtaaa gtccaccacc atggtccacg agatcgtcat gatgttgttc
      721 ttctttccgt catgtgtcgt aatgaggaca accggaccgg attcgatcaa cgtaaacgct
      781 ttaccgagct gcatttttct catgtgcgca cacgctcctt tccctgttgg cccgaccatg
      841 ccgacgccgt ccgtctttca aacccggatt gccgaagacc cggcgctacg gccggtcata
      901 tttccgtttg tacttttcat gctgccgctc gagaatctct ctgccgcgct ccgacccgtg
      961 cgccggaaga taaagagagc agcccgtctt cagcagcttt tcccagcttg ccaccatgcg
     1021 cttctcatcc gccgcgacgg gaggaatcgc cgatccctta aagacgccga acagggcatc
     1081 gccgacgatc gcgatttcgt tttcaaggat gacgctgagg gacccgggcg aatgacccgg
     1141 cgtggaaaga agataccctt gaaaccccag aggctttaaa tcgtaccgtt ccgcgaccgt
     1201 caggtcgccg tcgacgggcc ggtagcggaa gaactgtgaa agataccagt gcgacaggcg
     1261 gtcagagatc catccggtca gcggcagcgt tccgtggatc acgggatttt cgcccctgcg
     1321 cacgtagtcc gcttctttct tgtggacaat gatttttggg ttgtagcgat ctttgatcgc
     1381 cgccgcgttt tccgcgtggt cgtaatgggt gtgcgtcaga accaggccgg ccagcgagcc
     1441 gccgtttacg cccagttcat ccaattcccg ggagagcttt ttccagcttc cctttctgca
     1501 ggcatccacc agcagatgcc ggccgttcaa ggaaacgaga aagcagttgt acatgccgtg
     1561 ggaaagcctg cggacaacct gcccgccttt tgtcgtccat tgcttcatcc cttcctcctt
     1621 cggccgaaaa tcgtttgcgt tgcggacgtc cgtctacaac tacagcccga atcgtgacgc
     1681 cccggcctct ccccgacgca tcacgtccgc cggaaccacc cgcacgcccg gcgtcaactt
     1741 gccggcagcg gggaaggacg ccttacctga tgcatctacc acaaaccgaa gcggttttca
     1801 ggcattttca tcgctttgag gacagggtcg cacccggcac cggcgggtgg atttgccttg
     1861 acaatagaac gtacgttcta tatagagagt ttccggagaa atccgaagaa atcccggccg
     1921 tggaagcggc ttcaccctct tacaccccgg gaagaaaaaa acaggaaagc attgaagatg
     1981 attgagattt ttatcaaccg gtccagcggc cggcttcagg ccgcgcccat acggatcacc
     2041 ttaaaatcac accggagcag gcatgctgcc gctttccgcc agactctttt tttcaaaatc
     2101 gcggggttgc cggtcagcgg ggaagtttgc gccggcttcc cttctcccgc ggaagaggag
     2161 ctgcgcggtc atatttcttt tgacgagtat ctggtggcgc atccggaatc ctcgtttctg
     2221 ctctccgtca cgggcgattc catggccggt gccgggatca tggacaagga ctgggtcatc
     2281 attgaaaaaa accggcggcc gaaaaacggg gatatcattc tggcggaagt ggacggcgcc
     2341 tggacgataa aatactaccg caaaaaggga acccgcatca cactggaaac ggccggcgcc
     2401 ggagatcctc cgatggtccc gcgtgaggag ctgcgcatcg cgggagtggt aacggcgctg
     2461 gtgaggaagt atcatcgata aggcaagaag cgatggtgga tggtgaatgg tggatggtgg
     2521 atgccccgtc atggttcgac tggctcacca tgacactctt tttacccact acccaattac
     2581 ccattaccta tgacctatgg tccctgaccc ctgaccccta acctatggtc tgatttttta
     2641 tgtccgattt aattttttct cttgtttcct ggccgcgcgc cattgtccac attgacggcg
     2701 acgccttttt tacgtcctgt gaagaggcgg tgcacccgga gcttcggggc aagccgctca
     2761 ttaccggcgg cgagcgcgga atcgtcgcct gcgccagcta cgcggccaag cggctggggg
     2821 tcaaacgcgg cgtgccgctt tccgatgcgc gcaaaatgtg tccggacttg atcattctgc
     2881 cgtcggatta cgaaacctac agcctctttt cccggcggat gtttgccgtc
//
LOCUS       DAOSIM010000246         2923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_29543_length_2923_cov_5.769526, whole genome shotgun sequence.
ACCESSION   DAOSIM010000246 DAOSIM010000000
VERSION     DAOSIM010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29543_length_2923_cov_5.769526"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PLR47_09315"
     CDS             complement(<1..192)
                     /locus_tag="PLR47_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HOG13187.1"
                     /translation="MNPHVFREYDVRGIVDKDLNEVFIVNLGRAIGTYALQHKVRTMT
                     VGRDCRLSSDDYRRFLIAGL"
     gene            complement(289..975)
                     /locus_tag="PLR47_09320"
     CDS             complement(289..975)
                     /locus_tag="PLR47_09320"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG13188.1"
                     /translation="MAPILFDPSAIDTVIFDFDGTLARLNIDFEQMRRDVASLIDRWG
                     VDPRSLQNRFVLEMIGEVQALLGKNSGDHAASFTREAFRIIEEIEVEAAQSGSLFDGA
                     KELFTGLRKASLQAGIITRNCEKAVRTVFPDIHDYCPVVVCRNDVRRVKPHPEQINLA
                     LSRLGAKPGRCVMIGDHPIDIETGKNAGTRTAGVLTGRFQEKDFLRAGADMVLREAPD
                     ILRRLKPEDF"
     gene            complement(980..1819)
                     /locus_tag="PLR47_09325"
     CDS             complement(980..1819)
                     /locus_tag="PLR47_09325"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOG13189.1"
                     /translation="MTMDLTLLRDKDAFPLYVLERLNRTPVDYLEKHLSIERSNAAGG
                     KHLEAGVVLLLLFKNSEFHFLLIKRSNAVAQSGDISCPGGMLERPVDEMLSHIVSKTG
                     ILRTAGNRALHELPNKDGETQSLIRLFLMNALRESWEEIGLSPLNVEFLGALPTYSLT
                     LFTRTIFPLAGLVREPWPYRPNDEVDKMLEIPVSDFFRSSSYALLELSPENGTSDPRR
                     NFQFPCLVIPDGKGGEDILWGATFFIIANFLREVTGGAFPAETPGRIVTKILSPHYTS
                     GNR"
     gene            complement(1816..2808)
                     /locus_tag="PLR47_09330"
     CDS             complement(1816..2808)
                     /locus_tag="PLR47_09330"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOG13190.1"
                     /translation="MTAGNGNPSATFQPRSFLKNRHLQSVLASSHLMIPPHGPLRRHS
                     REFIIETPGGSKLLSYLSHHPRSRGMMILLHGWEGSSSSAYILAAGAYFFDRNFSVCR
                     LNLRDHGDSHHLNQGLFHGALIEETFDAVNAVARMAGNLPVYLLGFSLGANFSLRIAM
                     RHSRTPVGNLQKVFAVSPPLDPYRTTQTIDRGPALYRNYFLRKWKRSLRKKQKFFPDQ
                     YDFAGMLRARSCMELTEKMMAYFPEFPSYRDYFRLYTLTDNSFCDLGVPVKIFFAADD
                     PVIALDDFRKLRNHGFLTVSEQPFGGHCGFVDLFPRRRWYNEQIFEEFQQERNA"
BASE COUNT          664 a          767 c          874 g          618 t
ORIGIN      
        1 caaccccgcg atcagaaaac ggcggtagtc gtccgaactc aggcggcagt cgcgcccgac
       61 ggtcatggtc cgaactttat gctgaagcgc atatgtgccg atggcgcgtc ccagatttac
      121 gataaagacc tcgttgaggt ccttgtcaac gatgccgcgc acgtcgtatt cacgaaagac
      181 atgtggattc attttttccc tcaaaatcaa agaagatgat gcgtccgggc ttccggcgcc
      241 gtccgcttct atacaattta tccggggatg tcaacccggg aaggtcgttt aaaaatcttc
      301 aggcttcagc cgcctgagaa tgtccggggc ttccctcagc accatgtccg ctcccgcccg
      361 cagaaaatcc ttttcctgaa agcggccggt caggacaccg gcggttcggg ttcccgcgtt
      421 tttccccgtc tcaatgtcga tcgggtggtc gccgatcatc acgcatcggc cgggttttgc
      481 gcccagcctc gacagggcga ggttgatctg ctcggggtgc ggcttgacgc gccggacgtc
      541 attgcggcag acgaccaccg gacaataatc gtgaatatcc ggaaagacgg tccggacggc
      601 tttctcacag ttgcgggtga tgattccggc ctgaagggag gctttcctga gacccgtgaa
      661 cagctctttg gcgccgtcaa acagggagcc gctttgcgcc gcttccactt caatctcttc
      721 gatgattcga aaggcttccc gcgtgaacga agctgcatgg tctcctgaat tttttccaag
      781 cagggcctga acttcgccga tcatttccaa aacaaaccgg ttttgcagac ttcgggggtc
      841 cacgccccag cggtcaatca gtgaggccac gtcccggcgc atttgttcaa aatcgatgtt
      901 cagccgggcc agtgttccgt caaaatcaaa aatcacggtg tcgatggccg aaggatcgaa
      961 aagaataggg gccataggtt caacggtttc ctgatgtgta gtgcggggag agaatttttg
     1021 tgacgatgcg cccgggcgtt tccgccggaa aggcgccgcc cgtgacctcc cgcaggaagt
     1081 ttgcaataat gaagaacgtt gcgccccaga ggatgtcttc gccgcccttg ccgtccggaa
     1141 tgaccaggca ggggaattgg aagttgcggc gcgggtcgga tgttccgttc tccgggctga
     1201 gctccaggag agcgtaggaa gagctgcgga aaaaatcgct gacgggaatt tccagcattt
     1261 tgtccacttc gtcgttgggc cggtagggcc agggctccct gaccaggcct gccagcggaa
     1321 agatggtccg ggtgaaaagc gtgagcgagt aggtgggcag ggcgccaaga aattcaacgt
     1381 tgagcgggct caggccgatt tcctcccagg attcgcgaag cgcgttcatc agaaaaagcc
     1441 gaatgagcga ttgggtctca ccgtctttgt taggcagttc gtgaagcgcc cggttgcctg
     1501 ccgttcgaag aatgccggtt ttcgaaacga tgtggctcag catctcatcg accggccgct
     1561 ccagcatgcc gccggggcag ctgatgtcgc cggactgggc gacggcattc gaacgcttga
     1621 tcagcaaaaa atgaaattcg gaatttttga acagcagcag cagcacaacg ccggcttcca
     1681 aatgtttgcc gcccgcggcg ttggagcgtt caatcgaaag gtgcttttcc aaatagtcaa
     1741 cgggtgtccg gttcaggcgc tccagaacat aaagcggaaa ggcgtccttg tcccgcagca
     1801 gcgttaagtc catggtcatg cgtttctctc ttgttgaaat tcttcaaaaa tctgttcatt
     1861 gtaccagcgc cgccggggaa acaggtccac aaaaccgcag tgaccgccga acggctgctc
     1921 ggaaaccgtc agaaaaccgt gattgcgaag cttccggaag tcatcgaggg cgatgacggg
     1981 atcatcggcc gcgaagaata ttttgaccgg cacgccgaga tcacaaaaag aattatccgt
     2041 cagagtgtat agccgaaaat agtcccgata ggaaggaaat tccgggaaat aagccatcat
     2101 cttctccgtc agctccatgc aggaacgcgc ccgaagcatg ccggcaaagt cgtattgatc
     2161 cgggaaaaac ttctgttttt ttctgagcga acgcttccac tttcgcagaa agtagttccg
     2221 gtaaagagcg gggccgcggt cgatggtctg cgtcgttctg taagggtcga ggggcggact
     2281 gacggcgaac accttttgca ggtttccgac gggggtccgg gaatggcgca tcgcgatcct
     2341 gagcgagaaa ttggcgccga gggaaaaccc caggagataa accggcaggt ttccggccat
     2401 tcttgcaacc gcgtttaccg cgtcgaaggt ttcctcgatc agcgccccgt gaaagaggcc
     2461 ttgattgagg tgatgggaat cgccgtgatc ccttaaattg aggcggcaaa cggaaaaatt
     2521 ccggtcgaaa aagtaggcgc cggcggccag aatgtaggcc gaagaggagg agccttccca
     2581 gccgtgcagc agaatcatca ttccccggga acgggggtga tgcgacagat aggacagcag
     2641 cttcgatccg ccgggcgttt cgatgatgaa ttccctggaa tgcctgcgca gggggccgtg
     2701 agggggaatc atcaggtgtg aagacgccag aacggactgc aagtgccggt tcttcaaaaa
     2761 agaccggggt tgaaaggttg cagaagggtt tccgtttccg gcagtcatga gtttcccgtt
     2821 ttattaaaat gatttgcaat atccaggcgt tgacatcgcg gaaggatgaa atggtggcgg
     2881 tgcctggatt tgaaccaggg acactacgga tatgagccgt atg
//
LOCUS       DAOSIM010000247         2916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_29642_length_2916_cov_5.307235, whole genome shotgun sequence.
ACCESSION   DAOSIM010000247 DAOSIM010000000
VERSION     DAOSIM010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29642_length_2916_cov_5.307235"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /gene="rpmH"
                     /locus_tag="PLR47_09335"
     CDS             complement(<1..126)
                     /gene="rpmH"
                     /locus_tag="PLR47_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOG13191.1"
                     /translation="MKKTLTNKSNTKRKRTHGFLVRMATKTGRQVLSRRRAKGRKR"
     gene            522..2393
                     /locus_tag="PLR47_09340"
     CDS             522..2393
                     /locus_tag="PLR47_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOG13192.1"
                     /translation="MALNPLLDSRDQRFVLFEALEADKLNRYERFADFDRDTYEATLD
                     LAEQIAVEQVYPANAQADKTGAIYDPAAKTVKVPEGFHAAMKSYREAGFSGLSNSPEF
                     GGTGMPETVYRAAMEYFCAAGVAFSSYDTLKVGACNLIIHHGSEELKKIYVEKMVAGQ
                     WGGTMCLTEPSAGSDVGALKTRAVRQADGTYKITGQKIFITAGDNDLYENIIHPVLAR
                     IDGDPPGTPGISIFIVPKFHVNPDGTSGKRNDVATTGIEHKMGIKGSATCSLSFGDND
                     QCVGWLLGQERQGMKIMFQMMNEARLDVSQQGLGTASSAYMHAVTYAKNRLQGQDPAK
                     KGDFTSVPIIRHPDVKRMLIWMKAQVEAMRMLTLLAAYSADMANVEKGTPEGREAQGL
                     LDFLIPLCKAGNTDLAWLVTSEAMQVFGGYGYCSEYPIEQLARDCKILAIYEGTNGIQ
                     SMDLTMRKLLMNPDMFNFKVFKKRIALSCEKAGGIVEDKYIAAMKNAVDAMDATVAKL
                     TEWRDRKRILDILAVAQPLQQAMRMVAHAWMHLWSMTVAIPKLKAIAGDIKGEKAASL
                     AKDNAEAAFYMGKVLSGQFYLGAELKHLTGYLDYVTAGEPAAVNECFENIFTGALPQ"
BASE COUNT          712 a          752 c          785 g          667 t
ORIGIN      
        1 tctctttctg cctttggctc ttctgcggct cagcacctgc ctgccggttt tggtcgccat
       61 gcggaccaga aatccatggg tcctttttct cttggtattg gatttattcg tcaaagtctt
      121 tttcatgtta aacccctaaa agtaataggg cattgcctaa accacagtca ccttcatttg
      181 tcaaggtcaa agtggtcctg ttcacgcagc ttttccgccg gttggccgat tgacggggcg
      241 ctggtccgca ttcggaagtc gtttttgagt ttttccgcag atgccggttc atctgcggcg
      301 ggtccaattc catccaatcg gctttttaaa gaagattcca ttcggcatcg gacagccagc
      361 aacgctcgcc cgccggattc gagatcgatg atgacttttt ttctcaagtg tgctagggag
      421 ccctcacaca aaatatcgta ttgcggcaaa aaaagacagt cttcgcgtcc attgaaattt
      481 caggcgctgc gggctaaaaa tatcaaaaaa ggagtgtaat catggcatta aatccgttac
      541 tcgattccag agatcagagg tttgtcctgt ttgaagcgct ggaggcggac aaactcaacc
      601 ggtatgagag gtttgctgat ttcgaccgcg acacgtatga ggcgacactg gaccttgccg
      661 agcagattgc cgtcgaacag gtctatcccg cgaacgcgca ggccgacaaa acaggcgcga
      721 tatacgaccc ggccgccaaa accgtcaaag ttcccgaagg ttttcatgcg gccatgaaaa
      781 gctacaggga ggcgggattt tcgggtctga gcaactctcc cgaattcggc ggcacgggca
      841 tgcctgaaac cgtctatcgc gctgcgatgg agtatttctg cgcggccggc gtggccttca
      901 gctcgtatga tacgctgaag gtcggcgcct gcaacctgat cattcatcac ggttccgagg
      961 aactcaagaa aatctatgta gagaaaatgg ttgcaggcca gtggggcgga accatgtgcc
     1021 ttaccgaacc ttccgccggc tccgatgtcg gtgcgctgaa aacccgcgcc gtccggcagg
     1081 ccgacggcac ttacaaaatc accggccaga aaatattcat cacggcgggc gacaatgatc
     1141 tgtacgaaaa catcattcat ccagttctgg cgagaatcga cggcgatccc cccggaaccc
     1201 ccggcatctc cattttcatc gttcccaaat ttcacgtcaa tcccgatggc acgtcaggca
     1261 agcgcaatga cgtcgcgacc accggcatcg aacacaaaat gggcatcaag gggtcggcca
     1321 cctgttctct ctctttcggc gacaacgacc agtgcgtcgg ctggcttctg ggtcaggagc
     1381 gtcagggcat gaagatcatg ttccagatga tgaacgaagc gcgtctcgac gtttcacagc
     1441 agggtctggg cacggccagc tccgcctata tgcatgcggt gacgtatgcg aaaaaccgtc
     1501 tccaggggca ggacccggcc aaaaagggtg attttacctc cgtgccgatc atcaggcatc
     1561 ccgatgtgaa aagaatgctc atctggatga aggcacaagt ggaagccatg agaatgctga
     1621 cgctgcttgc cgcctactcg gcggacatgg cgaatgtcga aaaaggaaca ccggaaggcc
     1681 gcgaggcgca ggggcttttg gatttcctga ttcccctgtg caaggccggc aacacggacc
     1741 tggcctggct tgtcacttcc gaggccatgc aggtcttcgg cggctacggc tactgctcgg
     1801 agtatccgat cgaacagctc gcccgcgact gcaagattct ggcgatttat gaaggaacca
     1861 acggcattca gtcgatggat ttgacgatgc gcaagctgct catgaatccg gacatgttca
     1921 atttcaaggt tttcaaaaag agaatcgccc tgagctgcga aaaggccggg ggcattgtgg
     1981 aagacaaata tatcgcggcc atgaaaaacg ccgttgacgc gatggacgca accgtggcga
     2041 aactgacaga atggagagac cggaaaagga ttctcgatat tctggccgtc gcccagccgc
     2101 tccagcaggc catgcgcatg gttgcgcacg cctggatgca tttgtggtcc atgacggttg
     2161 ccatcccgaa actgaaagcc atcgctggcg acatcaaggg agaaaaagcg gcatcgctgg
     2221 caaaggacaa cgcggaagcc gccttctaca tgggcaaagt cctgtccggt caattctatc
     2281 tgggtgccga gctgaaacat ctcaccggct atctggatta tgtaacagcc ggcgagcccg
     2341 cggccgtcaa cgaatgtttt gaaaacatct ttaccggggc gttgccccag taggaaggcc
     2401 gccgccgcac gctccctgat gcctgctttg aaaagccccc ccccgacgct ttcacgcggg
     2461 gggattttct caaaggatcc gccggaggga caatccataa gtacatggaa agacgagttt
     2521 aatcagattt tggaaaataa tcgggaaata atgcaatttc ttcttgacag ttttgaacat
     2581 agagagtaga agctcacttc tgtccgtcag agatgtgctt gaaggcccta gcagttttca
     2641 agaggtttac cgatggtgcc actctttgtt gtcggatgat tcctgtttgc ggacggaaca
     2701 aaatttgaaa tttcagcttg acaatgaaat cgaattgatt taaatgtcac agctcgtttc
     2761 aagaaggctc tttggaaatt gaatagtggg aaaagaaatt tgtgggtcct agtagttttt
     2821 tgaagtatca agcgagttca aaaggaagat ttatcttcct ttacaggatt ttaaactgga
     2881 gagtttgatc ctggctcaga actaacgctg gcggcg
//
LOCUS       DAOSIM010000248         2913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_29672_length_2913_cov_15.303709, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000248 DAOSIM010000000
VERSION     DAOSIM010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29672_length_2913_cov_15.303709"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="PLR47_09345"
     CDS             <1..1105
                     /locus_tag="PLR47_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOG13193.1"
                     /translation="AVLSGVKEIRQVIDDARELWEAKKQKTILFVDEIHRFNKAQQDA
                     FLPHVESGLITLIGATTENPSFEVIAPLLSRTRVMLLKQFTEEDLMAILKRALSDPQR
                     GLGKFAIQIDDETIRFIVGISDGDARTALNNLEAAASMVQSLPEEERTITAKSAEEAL
                     LKKALLYDKDGEEHYNLISALHKSMRGSDPDATLYWLGRMLAAGEDPLYILRRMVRFA
                     SEDIGNADPHALVLTMAAQQAFHFIGLPEGELAIAQAAVYLAVAPKSNSLYTGYGKTK
                     DLINKTGYLPVPLHIRNAPTKLMKELEYGKDYKYAHDYQDAFVPQEYFPDKLQGRVLY
                     SPTDAGYEKIIRERVEEWRRRKNKAKKESEKKG"
     gene            1564..>2913
                     /locus_tag="PLR47_09350"
     CDS             1564..>2913
                     /locus_tag="PLR47_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOG13194.1"
                     /translation="MRIFQKKETIVQRGKRPYKGKFLQPLAVGIVSAVLVALILVMGY
                     LDIRRNETNLVGFMEDQALTTISVLLRQTEDNLKSIAAAKGKMPSHLKTAKEAESAYS
                     IQLVYDAITKLGQQIDEQWKKKLMSNAYLQKFAEDHYFWYVAILNMRGDILYQSGPLK
                     TDMLSKSDLFINGRKATTVELLEKIRIKEDIGYVGVKLKRGGGIAVFCLDRKGRLYWS
                     LKVAVERAMSKMGEGHGIAYLQVFNDQKILLGNIGELPAGLTSRDLNDQEMMKGKKGI
                     LSRKVDPQGIKILDVAAPFILENPYLPKEKIAGIVRIGLDRGSMDKIIAENRQNIFIF
                     MLFVVGIALLSMWLLYHDQNRHLAGIIDMERRLEKAERLSSLGQLAAGVAHEIRNPLN
                     AISMATQRLKRDFIPPDETKAREFKNLSTVIRDEIRRLNEIIEEFLSFSKSRRLELSN
                     "
BASE COUNT          814 a          723 c          737 g          639 t
ORIGIN      
        1 cgccgtcctt tccggcgtca aggaaatccg gcaggtgatt gacgacgccc gggaactctg
       61 ggaggccaaa aagcaaaaaa ccattttatt tgtcgacgaa atccatcgtt ttaacaaggc
      121 ccagcaggac gcttttttgc cgcacgtgga aagcgggttg attacgctga tcggcgcaac
      181 cacggagaat ccctcttttg aagtcatcgc gcctcttttg tccagaacgc gtgtgatgct
      241 gctcaaacag tttacggaag aagacctgat ggccattctg aagcgtgcac tttccgatcc
      301 gcaaagaggt ctgggcaaat tcgccatcca aatcgacgac gaaaccatac ggtttatcgt
      361 ggggatttcc gacggcgacg ctcgaacggc gctcaacaac ctggaggcgg cggcctcgat
      421 ggtccagtcg ctgcctgagg aagaaaggac gatcacggcg aaatccgccg aggaagcgct
      481 gctgaaaaaa gcccttctgt acgacaagga cggagaggag cattacaatc tgatttccgc
      541 gcttcataaa agcatgcgtg gaagcgatcc ggacgcaacg ctctactggc tgggccgcat
      601 gctggctgcc ggagaggacc cgctttacat tctgcggcgc atggtgcgct ttgcctccga
      661 agatatcggc aacgccgatc cccacgccct ggtgttgacg atggccgccc agcaggcgtt
      721 tcatttcatc ggcctgccgg aaggcgaact ggccatcgcg caggcggccg tctatctggc
      781 cgtggcgccc aaaagcaatt ccctgtacac cggctacgga aaaaccaagg atttaattaa
      841 caaaaccggg tatcttcccg ttcccctgca catccgcaac gccccgacga aactcatgaa
      901 ggaactggag tacggcaagg actacaaata cgcgcatgac taccaggatg cttttgttcc
      961 ccaggaatat tttcccgaca aacttcaggg aagagtgctt tactccccga ccgacgccgg
     1021 gtatgaaaaa atcatcagag aaagagtcga agagtggcgg cgccggaaaa acaaagcaaa
     1081 aaaggaatcg gagaaaaaag gataaaaaaa cgggaggctt gttggaagcc tcccgtcagg
     1141 agagaagagt gaaggcctgg ttaccttccc gggttatttt tgctgtcggc taatcatgcc
     1201 cgtgatattt tagtcgacac ttatatcttt ttctgattgg ttatctagca gataccatgc
     1261 cacagaggga tttatattgt aagttattga tttgtaaaaa gattaataaa taaaccacct
     1321 tctttgcttc catttcaaga caaaaccttt gacaacaaat gtccaattga cgatcgataa
     1381 aaaaatttat caagtaatat tagatagatg aacgaaagac aatttttgtc cacatttgga
     1441 caaaaatgtc cacctgagca gtattccatg ttgacttttt tcttgaaaag cacaatgatc
     1501 actggaaaac gtacaaatta gatttcaaaa gcgtttttca tgtgcgcaca aaataaggac
     1561 aacatgagaa tttttcaaaa aaaagaaact attgtccaac gtggcaagcg tccctacaag
     1621 ggaaaatttc tccagccgct ggccgtcggc atcgtgtcgg cagtcctggt cgctctgatt
     1681 ctggtgatgg gctatctgga tatccgccgc aacgaaacca acctggtcgg atttatggaa
     1741 gatcaggccc tgaccaccat cagcgtcctt ttgcgccaga cggaagataa tctgaaaagc
     1801 attgccgccg ccaaagggaa aatgccgtcc catctcaaaa cggccaaaga ggcggagtcc
     1861 gcctattcca tccagctggt ttacgatgcc atcaccaaac tggggcaaca gatcgacgaa
     1921 cagtggaaaa agaaattaat gagcaacgcc taccttcaaa agtttgcgga agatcattat
     1981 ttttggtacg tggccattct gaatatgcga ggcgatatcc tctatcaaag cggtcccctt
     2041 aaaaccgaca tgctgtcgaa aagcgatttg ttcatcaacg ggcgcaaggc aacgaccgtt
     2101 gaactgctgg aaaaaatccg catcaaggag gacatcggat acgtcggagt aaaactaaaa
     2161 agaggcggtg gaatcgccgt cttctgtctg gaccgaaaag gcaggttgta ctggagtctg
     2221 aaagtcgccg ttgaaagggc catgagcaaa atgggcgaag gccacggcat cgcctacctg
     2281 caggtcttta atgatcaaaa aatcctgctg ggaaatatcg gagaattgcc ggccggtttg
     2341 accagccggg atctcaacga tcaggaaatg atgaaaggga aaaaaggaat cttgagcagg
     2401 aaagtggacc cgcagggaat caagattctg gatgtagccg ctcccttcat tctggaaaac
     2461 ccctatctgc cgaaagaaaa gattgccggg atcgtccgca tcggtctgga ccgcggttcc
     2521 atggataaaa tcattgccga gaaccggcag aatattttta ttttcatgct gttcgttgtc
     2581 ggcatcgctc ttttgtccat gtggctgctc taccacgatc agaaccggca tctggccggc
     2641 attatcgaca tggagcgccg gctggaaaag gccgagcgtc tttcttcgct ggggcagctg
     2701 gccgccgggg tggcgcacga aattcgcaac ccgctcaacg ccatcagcat ggcgacccag
     2761 cgcctgaaaa gagatttcat ccctccggac gaaacaaagg cccgggagtt taaaaatctt
     2821 tccacggtca tccgcgatga aatccggcgc ctcaatgaaa tcatcgaaga gtttctttct
     2881 ttttccaaga gcagaaggct ggagttgagc aat
//
LOCUS       DAOSIM010000249         2911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_29703_length_2911_cov_5.764006, whole genome shotgun sequence.
ACCESSION   DAOSIM010000249 DAOSIM010000000
VERSION     DAOSIM010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29703_length_2911_cov_5.764006"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /gene="lptD"
                     /locus_tag="PLR47_09355"
     CDS             <1..697
                     /gene="lptD"
                     /locus_tag="PLR47_09355"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HOG13195.1"
                     /translation="HEIKPEIFYSYIPSVNQDNLPDFIPWVNSYMANSLSSNIGYTNV
                     LTEQNAVAWALTNTLTARVQGKSGSRSYFELLRFKLFQVYDINEAKKDMAGSTTERRP
                     VSDLGIELDLKPHPYIAFAARNKYNPYVGWKEMNYDLGLHDWRGDNLTVGYRYTLDSL
                     EEINMALKLNLTRGVSGNLILRRDQFNDRIVENTFGLAYRAQCWGVGVDYTKTYDDER
                     IMLKFSLAGLGMF"
     assembly_gap    846..927
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <928..1267
                     /gene="rplM"
                     /locus_tag="PLR47_09360"
     CDS             <928..1267
                     /gene="rplM"
                     /locus_tag="PLR47_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOG13196.1"
                     /translation="EIALRLRGKHKAIYTPHVDTGDFIVVVNAEKITLTGKKLTDKIY
                     YSYSGYPGGLRETAAGKMLVSKPENLIRIAVAGMLPKNNLGRKMLKKLKVYSGNAHPH
                     EAQCPQTLTV"
     gene            1310..1702
                     /gene="rpsI"
                     /locus_tag="PLR47_09365"
     CDS             1310..1702
                     /gene="rpsI"
                     /locus_tag="PLR47_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOG13197.1"
                     /translation="MTVQRFYATGKRKSAIARVYMKSGSGSIMVNKRNYEEYFTRPSL
                     KMIIRQPLEITGKKDQFDFYVNVSGGGAAGQAGAVKHGISKALLECDAELRPILKKAG
                     FLTRDARIKERKKYGQPGARKRFQFSKR"
     gene            1748..2788
                     /gene="argC"
                     /locus_tag="PLR47_09370"
     CDS             1748..2788
                     /gene="argC"
                     /locus_tag="PLR47_09370"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOG13198.1"
                     /translation="MKTKAAIFGASGYTGQELIRILSAHPQVELVAVTSRRYAGVKVS
                     DVFPSLSGLTFLSYTNASPREIAKLCDVVFLALPHGVSMEIAPVFIEAGKKVIDLSAD
                     YRLRDLATYEQWYAKHTSAGLFGQAVYGLPELYRETVKTSPLIANPGCYPTSIILGLA
                     PALKNKLLEVSTIIADSASGVSGAGRDPVVGSLFCEVDGGFKAYKVGKHRHTPEIEQE
                     LHQLAGEKFVISFTPHLLPVKRGILSTMYATLKKEVTEAELRDLYASFYAGEKFVRIC
                     PAGAYPNISSVCGSNFCDIGLTVDPRTGRVIMISAIDNLIKGAAGQAVQNMNLVCGLP
                     EDSGLGTPPLYP"
BASE COUNT          698 a          706 c          781 g          644 t
ORIGIN      
        1 acatgaaatc aagcctgaaa ttttctattc gtacattccc agcgtgaacc aggataatct
       61 tcccgatttc attccctggg taaattccta catggccaac tctctgtcat ccaatatcgg
      121 ctataccaat gttttaacgg aacaaaatgc ggtggcctgg gcgctgacca atactctgac
      181 cgcaagggta cagggcaaat ccggatcgcg cagctacttt gaattattgc gttttaaact
      241 atttcaggtg tacgacatca acgaagccaa aaaagacatg gccggttcca ccacggagag
      301 gcggccggtc tcggatctgg ggatcgaact tgatttgaag ccacaccctt acatcgcctt
      361 tgccgcgcgc aataagtaca atccttatgt aggctggaag gagatgaatt acgatctggg
      421 actccatgat tggcgcggag acaatctgac ggtaggatac cgctatacgc ttgattctct
      481 tgaggaaatt aacatggccc taaagctcaa tctcacccgg ggggtgagcg gaaatctgat
      541 tctcaggcgg gatcagttta atgatcggat agtggaaaat acgttcggtc tggcctatcg
      601 agcgcaatgc tggggcgtgg gagtggatta taccaagacc tacgatgacg aaagaatcat
      661 gctgaaattc tccctggccg gattgggcat gttttaaatt gtgacgccct gcttgtccgg
      721 cgctgccttt tcccgtggag ggttggaagg cggcatgacc ctgtcacagg ctgcggcaaa
      781 ggaattcaaa tattttccaa acattttcct tgacaaagag cgtggaattg catagcttcc
      841 accttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnntga aatcgccctg cgccttcggg gcaagcacaa
      961 agcgatttat acgccgcatg tggataccgg cgattttatt gtcgtggtca atgccgaaaa
     1021 gatcacgctg accggcaaga agctaacaga taagatttat tattcctact ccggttaccc
     1081 gggggggctc agggaaacgg cggccgggaa gatgttggtt tccaaaccgg aaaatctgat
     1141 tcgaattgcc gtggcaggga tgctgccgaa aaacaacctt ggcagaaaaa tgttaaaaaa
     1201 actaaaggtt tatagtggaa atgcgcatcc gcatgaagcc cagtgtccgc aaaccctgac
     1261 ggtttgatga tccgggctgt ttatggatta atttattttg ggagaggtca tgacagtaca
     1321 acggttttat gcaacgggga aaagaaagag cgccattgcc cgcgtttata tgaaatcagg
     1381 cagcggcagc attatggtga acaaaagaaa ctatgaagaa tattttacgc gtcccagtct
     1441 gaaaatgatc attcgccagc ctctggagat cacggggaag aaggaccagt tcgactttta
     1501 cgtcaatgtc agcggcggag gcgcggcggg tcaggccgga gcggtgaagc atgggatttc
     1561 caaagcgctt ctggaatgcg acgccgagtt gcgccccatc ctgaaaaaag cgggttttct
     1621 gacgcgagac gcccgcatca aggagagaaa gaagtacggc cagccgggcg cccgcaagag
     1681 attccaattc tccaaacgtt aatcattcaa ggggggcctt caggctcccc ttttttttgg
     1741 agtttttatg aaaacgaaag cagcgatatt cggcgccagc ggctatacgg ggcaggaact
     1801 cattcggatt ctgtccgcac atccacaggt ggagctggtt gccgttacct ccaggcgcta
     1861 cgccggcgtg aaggtctcgg acgtttttcc gtctctttcc ggcctgacat ttctctcgta
     1921 caccaacgct tcgccccggg aaatcgcaaa gctttgcgat gtcgtttttc tggcgctgcc
     1981 gcacggcgtg tccatggaga tcgccccggt ctttatcgag gcggggaaaa aggtgatcga
     2041 tctgtccgcc gactaccgcc tgcgggattt ggcaacctat gaacagtggt atgccaaaca
     2101 taccagtgcc ggtctgttcg gacaggcggt ttacgggctt ccggaacttt accgggaaac
     2161 cgttaaaaca tcgccgttga ttgccaatcc cggctgttat ccgaccagca ttattctggg
     2221 gcttgcgccc gcgctcaaaa acaaactcct ggaggtttcc acgatcatcg ccgattcggc
     2281 ttccggggta agcggagcgg gaagggaccc ggtcgtcgga tcgctttttt gtgaagtaga
     2341 cgggggcttt aaagcctata aggtcgggaa gcacaggcac accccggaaa tcgagcagga
     2401 actccatcag ctggccggtg aaaaattcgt catttccttc acaccgcatt tgctgccggt
     2461 caaaaggggc attctgagca cgatgtatgc gacgctgaag aaagaggtga cggaggcgga
     2521 gctgcgggac ctgtatgcat ccttctacgc cggggaaaaa ttcgtgcgga tttgtcccgc
     2581 gggcgcctat cccaacattt cctccgtttg cgggtccaac ttctgcgaca tcggcctgac
     2641 cgttgatccg cggacgggcc gggtgatcat gatttccgcg atcgacaatc tcatcaaagg
     2701 cgccgccggc caggccgtgc agaacatgaa tctggtttgc gggctgcccg aagacagcgg
     2761 tctcgggacg cccccgctgt atccctgacg aaggcaagaa aatgacccag aaaatctata
     2821 atacgaagac cagaaaaaaa gaaatctttc agccgatccg ggaaggcgcg gtcgggatgt
     2881 acgtttgcgg cattacggcg tatgatgtct g
//
LOCUS       DAOSIM010000250         2878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_30129_length_2878_cov_9.591215, whole genome shotgun sequence.
ACCESSION   DAOSIM010000250 DAOSIM010000000
VERSION     DAOSIM010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30129_length_2878_cov_9.591215"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PLR47_09375"
     CDS             complement(<1..810)
                     /locus_tag="PLR47_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG13199.1"
                     /translation="MNNSGLTPREIVERLDRHIIGQADAKRAVAIALRNRWRRQNVPE
                     DLADEISPKNIIMIGPTGVGKTEIARRLAKLDNSPFLKVEASKFTEVGYVGRDVESMV
                     RDLVELSINMLKAEEQDHVRARAAEIAEDRLLDLLLPPRPAERNVGDMLTDAEPEQAS
                     LPLNEEAARRQDATREKLRKLLHDGKLDERMVELEIAEPRSGPMIEIFSAAGMEDMGL
                     NLKDMLGSMMPQKKKKRNVKVPEALEIIASEEAQKLIDMDKVTKTALDRVEQ"
     gene            complement(829..1404)
                     /gene="hslV"
                     /locus_tag="PLR47_09380"
     CDS             complement(829..1404)
                     /gene="hslV"
                     /locus_tag="PLR47_09380"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007302170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOG13200.1"
                     /translation="MKIRGTTILAIRKGDKITVAGDGQVTLDTTILKHGARKVRRLYN
                     NEVIVGFAGATADAFTLFDRFDQKLEQYNGNLLRAAVELTKDWRTDRVLRHLEALMIA
                     VSRDYSLIISGNGDVIESDDDVMAIGSGGAFAQAAARALVRHSNLTATEIAREAMKIA
                     SEICIYTNDHVTIEEIDLSEEKSKEDKKSKK"
     gene            complement(1518..2426)
                     /gene="xerC"
                     /locus_tag="PLR47_09385"
     CDS             complement(1518..2426)
                     /gene="xerC"
                     /locus_tag="PLR47_09385"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOG13201.1"
                     /translation="MKELLAAFRTFLDVQRNVSEHTRKAYLTDIEEYGEFLRNSSAQK
                     SVADADTDTVRSYMAFLHGRKLKKSSINRKISSLRAFYRFLVREGTVRNNPAQDVQTP
                     KTEKYMPTFLSVDEAFDLLEASGSDPSLSGLRNLAMLELFYSSGLRLAELAGLDEDDV
                     NFSAALVKVRGKGKKERIVPVGKRALEALKRYLSATEDTRRKLFREGMGGALFLNERG
                     GRITTRSIARIVDTATVKSGIGRKISPHALRHSFATHLLAAGADLRSIQEMLGHESLS
                     TTQKYTAVNINRLMEVYDKAHPRAKK"
     gene            complement(2433..2876)
                     /locus_tag="PLR47_09390"
     CDS             complement(2433..2876)
                     /locus_tag="PLR47_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOG13202.1"
                     /translation="MVILKDLQESKAIPIWIGLFEASAIATELEHVVFSRPMTHDLMN
                     EFLKALDVTVNRIEIVDIRNNTFFAAVYLVRDGQTYRVDARPSDAIALALRAGASIFV
                     EESVLEKSRNIDFGIKLADIDKFKEDKVREFLENLSAEDFGKYKM"
BASE COUNT          509 a          898 c          734 g          737 t
ORIGIN      
        1 ctgctccacg cggtccagcg cggttttggt gaccttgtcc atgtcaatga gcttctgcgc
       61 ctcttcggac gcgatgatct cgagggcttc cggcaccttg acgtttcttt ttttcttctt
      121 ctgcggcatc atgctgccca gcatgtcctt cagattgagt cccatgtctt ccatgccggc
      181 ggcggaaaaa atctcgatca tggggccgct cctcggctcg gcgatttcca gctccaccat
      241 gcgttcgtcg agtttcccgt cgtgcagaag ttttcgcagc ttttcgcgcg tcgcgtcctg
      301 cctgcgcgcc gcctcttcgt tgagcggcag actcgcctgc tccggttccg cgtccgtcag
      361 catgtcgccg acgttccttt ccgcgggccg cggcggcagc agcagatcca gcagccggtc
      421 ttccgcgatt tccgcggctc tcgcccgcac gtgatcctgc tcctcggcct tcagcatgtt
      481 gatcgaaagc tccaccagat cccgcaccat ggattccacg tcccgcccga cgtagccgac
      541 ttccgtaaat ttcgaagcct ccaccttcag aaacggggaa ttgtccagtt tggcaagcct
      601 tctggcgatt tccgttttgc cgactcccgt ggggccgatc atgataatat ttttcggcga
      661 tatttcatcc gccagatctt ccggaacatt ctgcctgcgc cagcggtttc gcagcgcgat
      721 ggccacggcc cgtttggcgt cggcctggcc gatgatatgc cggtccagcc gttccacaat
      781 ctcgcggggt gtcaaacctg aattgttcat ttttcttctg tgactccttt actttttgct
      841 ctttttgtcc tctttggact tctcctcact gaggtcgatt tcttcaatcg ttacgtggtc
      901 gttggtgtag atgcatattt ccgaggcaat cttcatcgct tctctcgcga tttccgtggc
      961 ggttagattg ctgtggcgca ccagcgcgcg ggccgcggcc tgcgcaaaag cgccgcccga
     1021 tccgatggcc atcacgtcgt catccgattc aatcacatcg ccgtttccgg aaatgatgag
     1081 cgaatagtcc cggctgaccg cgatcatcag cgcttccaga tgccgcagca cccggtcggt
     1141 ccgccagtcc ttggtcagct ccacggcggc gcgaagcagg ttcccgttgt actgttccag
     1201 tttctgatca aagcggtcaa acagcgtgaa ggcgtcggcg gttgcgccgg cgaatccgac
     1261 aatcacctcg ttgttgtaca gccttcgcac cttgcgcgcg ccatgcttca gaatcgtcgt
     1321 gtccagcgta acctgaccgt cgccggccac cgtgatttta tcgcccttac ggatcgccag
     1381 aatggttgtg cctctgatct tcatttggtc tcccatactt cgcagctcat aactgatggc
     1441 tcatagctta tggtttgttt ctcttcactc attgctcatc actcgtctct cgtcgctcat
     1501 tgctcgtcac tcatcgctta tttctttgct ctggggtgag ccttgtcgta cacttccatc
     1561 aggcggttga tgttgaccgc cgtgtatttc tgcgtcgtgg aaagactctc atgccccagc
     1621 atttcctgga tcgaacgcaa atcggcgccg gccgccagca gatgcgtggc aaagctgtgc
     1681 cgaagcgcgt gagggctgat cttccgtccg atgccgcttt tgacggtggc cgtatccacg
     1741 atgcgggcaa tgcttcgggt cgtaatccgc cctccccgtt cgttcaaaaa aagcgcgccg
     1801 cccatccctt cacggaaaag cttcctcctc gtgtcctccg tcgcggacaa atatcgcttc
     1861 agcgcctcca gcgctctttt gcccacgggg acaattctct cttttttccc cttgccgcgc
     1921 accttgacca gcgcagcgct gaaatttaca tcgtcctcgt ccagaccggc cagctccgcc
     1981 agcctgagcc ccgaagaata gaacagctcc agcatcgcca gattgcgcag accggacaaa
     2041 gacgggtccg agccggatgc ttccaggaga tcaaaggctt catcaaccga caaaaaagtc
     2101 ggcatgtact tctccgtctt cggcgtctga acgtcctggg ccggattgtt ccggacggtt
     2161 ccctcccgca ccaaaaacct gtaaaaagcc cgcagggagg atattttgcg gttgatggac
     2221 gactttttca gcttccggcc gtgcaaaaac gccatgtacg accggacggt gtccgtatcg
     2281 gcatccgcca cgcttttttg agcggatgaa ttgcgcagga actcgccata ttcctcgata
     2341 tccgtcagat aggccttgcg ggtatgctcc gacacattac gctggacgtc cagaaatgtc
     2401 cgaaaagcgg caagcagttc tttcatttct tttcacattt tatatttccc gaaatcttcc
     2461 gcggataagt tttccaaaaa ctcccttacc ttgtcttcct tgaatttgtc gatgtcggca
     2521 agcttgattc cgaaatcgat gttgcgggat ttttccagga cgctctcctc cacgaaaatc
     2581 gaggcgccgg ccctcagcgc cagcgcgatg gcgtcgctgg gacgcgcgtc aacccggtag
     2641 gtctgcccgt cccgaaccag atacacggcc gcgaaaaaag tgttgttgcg gatatcgaca
     2701 atttcaatcc ggttgaccgt gacgtccagg gccttgagga attcgttcat caggtcatgg
     2761 gtcatcggtc tggaaaacac cacgtgctcc agttccgtgg cgatggcgct ggcttcgaac
     2821 aggccgatcc agatcggaat cgctttgctt tcctgcaggt ctttcaggat gacgatcg
//
LOCUS       DAOSIM010000251         2866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_30302_length_2866_cov_7.453575, whole genome shotgun sequence.
ACCESSION   DAOSIM010000251 DAOSIM010000000
VERSION     DAOSIM010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30302_length_2866_cov_7.453575"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLR47_09395"
     CDS             complement(<1..223)
                     /locus_tag="PLR47_09395"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOG13203.1"
                     /translation="MSEGFLSIGELSRKLEMSQRTIRYYEEIGLLNSIKRVEGGRRIY
                     TEKDLRRLKLIKRLKIMGMTLSEMQELESM"
     gene            complement(278..1831)
                     /locus_tag="PLR47_09400"
     CDS             complement(278..1831)
                     /locus_tag="PLR47_09400"
                     /inference="COORDINATES: protein motif:HMM:NF006182.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-CoA synthetase"
                     /protein_id="HOG13204.1"
                     /translation="MSNICYRDTIGDMARRAATKYGDKIAIIFRDEKISFYELERQAR
                     RFANLMTSYGVKKGDRVAIYAYNSHYYPISMMGLAKIGAIQVPINYMLNAEEIAYVVN
                     HSGSRIFIVEDILYPIIAKDQAKFTTVEKWGFLPLGDSLVPDGFFNMITEMEAMTYDE
                     PQVEVSAEDIVQIPYTSGTESKPKGAMLSHRALMSQYHSCIFDGQYETDDVSLHALPL
                     FHCAQLHCFLMPYLYVGATNVIMHKADPLEMIRLIEKYQITHMFAPPTVWIGILNHPE
                     FKNYYHSSLKKAAYGASIMPVEIIKQLAVTFRGLKLWNYYGQTEMGPVATILKPEDQL
                     LKPGSAGKPVLNVETRLMDDDGKFVPLGEVGEIVHRSGHVMTGYLNDSEKTSEAFEFG
                     WFHSGDLGRFDADGYLYVVDRKKDMIKTGGENVASREVEEVLYQYPGIEEAAVIGLSD
                     PKWIEIVAAVVVPKKGASLIDKDIIAHCRERLAAFKCPKKVILLSRLPKNPSGKILKR
                     ELKEKFAAS"
     gene            complement(1939..>2866)
                     /locus_tag="PLR47_09405"
     CDS             complement(1939..>2866)
                     /locus_tag="PLR47_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG13205.1"
                     /translation="PVFHCFGCVMSTLNSVYHATAMVLLEYFEPKLSLESIHKEKCTA
                     INGVPTMFIAMLHHPDFERYDMTSLRTGIMAGAPCPVEAMNQVRTKMHCPEVVIAFGQ
                     TESSPVMTMTRRDDPVEMRVATVGRLLPDIEGKIVDPETGEDLPPNTQGEIVTRSACI
                     MKGYYKMPEATTAAIDADGWLHTGDLGEIDPNGYFKVTGRIKDMIIRGGENIYPRELE
                     EFIFTHPKVQNVQVVGIPDSKYGEQVLAAIHLKNGNRATAEEFIEFCKGKIARHKIPK
                     YWEFVDEYPMTASGKIQKFKIREIFEKKYKAD"
BASE COUNT          549 a          812 c          785 g          720 t
ORIGIN      
        1 acatggattc gagttcctgc atttcggaaa gcgtcatgcc catgatcttc agccttttga
       61 tcagtttcag gcgacgaagg tccttttcgg tgtagatccg gcgtccgcct tccacgcgct
      121 tgatggaatt gagcagaccg atctcttcgt aatagcggat ggtccgctga ctcatctcca
      181 gctttctgga gagctccccg atggacaaaa agccttcgct catgatcggc tccctttgct
      241 tttcaaagtc acgggcggcg tccgcccgcg gccggattta cgacgcggca aacttttcct
      301 tgagttcgcg ctttagaatt ttcccagaag gattcttggg cagtctggaa agcagaatca
      361 cctttttggg gcatttgaag gcggccagcc tttccctgca gtgggcaatg atgtccttgt
      421 cgattaacga agcgcctttt ttcggcacga ccaccgccgc cacgatttcg atccacttgg
      481 gatcggaaag accgatgacg gccgcttctt cgatgcccgg atactgatag agcacttcct
      541 cgacctcgcg ggaagccacg ttctcgccgc cggttttgat catgtccttc ttgcggtcca
      601 ccacgtagag atagccgtcg gcgtcaaaac ggcccagatc gcctgagtgg aaccagccga
      661 actcaaaggc ttcggatgtc ttttccgaat cgttgaggta gcccgtcatg acgtggccgg
      721 agcggtgaac gatttccccc acttctccca gaggaacaaa ttttccgtcg tcgtccatca
      781 gccgcgtttc cacgttcaga accggcttgc ccgccgaacc cggcttcaga agctggtctt
      841 ccggcttcag gatcgtggcc accggcccca tctccgtctg gccgtagtag ttccacagtt
      901 tgaggccgcg gaaggtcacc gcgagctgct tgatgatttc caccggcatg atgctggcgc
      961 cgtaggccgc ctttttcagg ctgctgtggt agtagttttt gaactccggg tggttgagga
     1021 ttccgatcca caccgtgggc ggagcgaaca tgtgcgtgat ctggtatttt tcaatgagcc
     1081 tgatcatctc cagggggtcg gccttgtgca tgattacgtt ggtggcgccg acatacagat
     1141 acggcatgag aaaacagtga agctgcgcgc agtgaaacag gggcagcgcg tgcaggctca
     1201 cgtcgtcggt ttcatactgg ccgtcgaaga tgcagctgtg gtactgggac atcagcgccc
     1261 tgtgcgagag catcgccccc ttgggcttgg attccgtgcc gctggtgtag gggatctgca
     1321 caatgtcttc ggcgctgacc tctacctgag gctcgtcgta ggtcatggcc tccatttccg
     1381 tgatcatgtt gaagaaaccg tcaggaacca gcgaatcccc cagcggaagg aagccccact
     1441 tttcgaccgt cgtgaatttc gcctggtcct tggcgatgat cggatacagg atgtcttcca
     1501 cgataaagat tctggagccg gaatgattga cgacgtaagc aatttcttcg gcattcagca
     1561 tgtagttgat gggaacttga atggcgccga tcttggccag gcccatcatg ctgatgggat
     1621 agtagtgcga attgtaggcg tagatcgcca cccggtcgcc ctttttgacg ccgtagctgg
     1681 tcatgagatt ggcaaagcgc cgcgcctgcc gctcgagttc ataaaaagaa atcttctcgt
     1741 cgcggaaaat gatggcgatc ttgtcgccgt atttggtggc cgccctgcgg gccatatcgc
     1801 cgatcgtgtc acgataacaa atattgctca tcttgaaatc ctttcgcttt tgttctcctg
     1861 ctttatccta tagcccatag cctatcgcct cttgcctgtt ttttttccat ccaccatcca
     1921 ctatccacca cagcttcttc agtctgcttt atacttcttt tcaaatatct cacgaatctt
     1981 gaacttctga atcttgccgc tggccgtcat cgggtattca tccacgaact cccagtattt
     2041 ggggatcttg tggcgcgcga tttttccctt gcagaattcg atgaattcct ccgccgtcgc
     2101 ccggttgccg ttcttcagat ggatggccgc gagaacctgc tcgccgtatt tggaatccgg
     2161 aatcccgaca acctggacat tttgcacctt gggatgcgtg aagatgaatt cttccagctc
     2221 ccgcggataa atattttcgc cgccccgaat gatcatgtcc ttgatccgtc cggtgacttt
     2281 aaaatagccg ttcgggtcga tctcgccgag gtcccccgtg tgcagccacc cgtcggcgtc
     2341 gatggcggcg gtcgtggctt ccggcatctt gtagtagccc ttcatgatgc aggcgctacg
     2401 cgtgacgatt tcaccctggg tgttcggagg caaatcctcg ccggtttcgg gatcgacgat
     2461 ttttccttcg atgtccggca gcagacggcc gaccgtggcc acgcgcattt ccaccggatc
     2521 gtcgcggcgg gtcatggtca tgaccggcga agattccgtc tggccgaacg cgatgacgac
     2581 ttcggggcag tgcattttcg tgcgcacctg attcatggcc tcgaccgggc agggcgcgcc
     2641 ggccataatg ccggtccgca gggacgtcat gtcatagcgt tcaaaatcgg gatgatggag
     2701 catggcgatg aacatggtcg gcacgccgtt gatcgccgtg catttttcct tgtgaatcga
     2761 ctccagggac agcttcggtt caaaatactc cagcagcacc atggcggtcg catggtaaac
     2821 gctgttgagc gtgctcatga cgcacccgaa gcaatgaaag acgggc
//
LOCUS       DAOSIM010000252         2864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_30336_length_2864_cov_9.266643, whole genome shotgun sequence.
ACCESSION   DAOSIM010000252 DAOSIM010000000
VERSION     DAOSIM010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30336_length_2864_cov_9.266643"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..746
                     /locus_tag="PLR47_09410"
     CDS             267..746
                     /locus_tag="PLR47_09410"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOG13206.1"
                     /translation="MKKRASRNDRENGFSMFEVIVVILLIGVVAAFVAVRGSSKTVYD
                     LASEAEILKGHLRYAQFRAMSDTESWGMSLSTNGYQLLKNKAPATDSLPNESGPSHAL
                     PSGITITTGAGTAIHFNERGIPVGDGDAPLTAETEIALSHGSATQTIFIKPETGFIP"
     gene            743..1177
                     /locus_tag="PLR47_09415"
     CDS             743..1177
                     /locus_tag="PLR47_09415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOG13207.1"
                     /translation="MKRYLANRRGFTLLEIIVTLTILSILGVMVFQFMRSSLVRSTEP
                     FSIVAKTFVLSEVAENITADYLQNIPRNVPALKASIGAEGSEHINSGYCPAATDCRYS
                     VVDNHYIAFDGNHEVPTTVQGLLKVTIRGEQNETITMVFIQP"
     gene            1189..1725
                     /locus_tag="PLR47_09420"
     CDS             1189..1725
                     /locus_tag="PLR47_09420"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOG13208.1"
                     /translation="MMKSSKKGFTLIELIFTLVLAGIIVSAVGMGIVHVVQGFMFTKE
                     NAAALQKGQMALSRISLELKKAAGISDASTATSISFDTYADTGGPTPTLVTQMIRLNG
                     DKVEFVGSSGNPNILTDRAAAGNGLVFRYYTHYNDPEASQCDPKEARIIAITLRLAAA
                     DGVVKTFTARVRPRNLPY"
     gene            1735..>2864
                     /locus_tag="PLR47_09425"
     CDS             1735..>2864
                     /locus_tag="PLR47_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13209.1"
                     /translation="MNLRQPHGSILINLIVITTIVAVLGAGMLYLTTTSTYASLFPNS
                     QARAYYLAEAGIAHAQQLLAQNIHNPSFYPGGHRGIDPPVTYTLANGDRFILTSYDFN
                     NGGTPDPTRVVVEAIGVVHAGTWMESKKKVTKSGILRDDPNPVDPTPSKKTEEFIDFM
                     VNDQVFVYGSAMSFSGNNVSGADATVVIRGPLDTDDTNLGASIAATTIYIDGHVELNK
                     GSAGLGSSSSPGNIYINGDLTLWNGSRSIYGDVYVNGNLRLKDAKIYGNIYVNGTVEL
                     GWTPTLSANANIYYVGDGNPDTPDIIHPKNYDQSILNKCHKIPYGAVQAFPSFSMPDV
                     AIPDPRGSAWFASKGYVSSGAPVSGLKIYTEGDYNSSTWRPTA"
BASE COUNT          718 a          771 c          726 g          649 t
ORIGIN      
        1 atcatatatt tggtaatttc ataacggcaa gcagaaagat gcgtgaaccg ggaagaggca
       61 gcaaatgcat cgggcgtatg cgtccggatg tcttgcagcc ggattttctg ttatgaagag
      121 agatgaagat tctgttataa aaagcagatt gttagtcttc ttgacatatc ttcttttctt
      181 ccataggatc ggcagtaacc attttaaggc ggctcgattg atgccgtgaa cctgtgccgc
      241 cggaaggccg gatggaagag acggagatga aaaagagagc ttcgcggaat gacagggaaa
      301 acggtttttc gatgttcgaa gtgatcgttg tcattttgct catcggcgtc gtcgctgcct
      361 ttgttgctgt ccgcggatca tcgaagacgg tttacgatct ggcgtcggaa gcggagatcc
      421 tcaaggggca tctgcgttac gctcaattca gggcgatgag cgataccgaa tcctggggaa
      481 tgagcctctc cacaaatggt tatcaactgc tgaaaaacaa ggcgcctgcc acggactcgc
      541 tgcccaatga aagcggcccc tcccatgctt tgccttcagg aatcaccatc acgaccggcg
      601 caggaacggc tattcatttc aacgaacggg gaattccggt gggcgacggc gacgcgccgc
      661 tgaccgcaga aaccgaaatt gccctgtccc atggatccgc cacacagacc atcttcataa
      721 aacccgaaac ggggttcatc ccatgaaacg ttacctggcc aaccgcagag gattcaccct
      781 cctggaaatc atcgtcacgt tgaccatcct gtccattctg ggcgtcatgg tgtttcaatt
      841 catgaggtct tctcttgtca ggagcacgga acccttttcc atcgttgcca aaacatttgt
      901 cctgagcgaa gtggctgaga atatcacggc ggactacctg caaaacatcc ctcggaatgt
      961 acccgcgctg aaagccagca tcggagcgga aggatcggaa cacatcaaca gcggatattg
     1021 tcccgccgcg acggattgcc gatattcggt tgtcgacaat cactacatcg cctttgatgg
     1081 gaatcatgaa gttccaacca ctgttcaggg attattaaag gtcaccatcc gtggcgaaca
     1141 aaatgaaacg atcaccatgg tttttatcca gccatgaagg aatgtcgaat gatgaaatca
     1201 tccaaaaaag gctttactct gatcgaattg atcttcacgc tggttttggc gggaatcatt
     1261 gtttcggccg ttggaatggg catcgttcac gtcgttcagg gattcatgtt cacgaaagag
     1321 aacgccgccg ctctacagaa gggacagatg gccctcagcc gtatttcact ggagttgaaa
     1381 aaagccgctg gcatttcgga cgcctcgacg gcgacatcga tttctttcga tacctatgcg
     1441 gacacgggcg gaccgacacc gaccctcgtg acgcagatga tccggttaaa cggcgacaaa
     1501 gtggaatttg tcggctcctc aggaaatccc aatatcttga cagatcgggc ggctgccggc
     1561 aacggcctgg tctttcggta ctacacccat tacaacgatc cggaagcaag tcagtgtgac
     1621 ccgaaggaag ccagaataat cgccattacg ttgcgtcttg ccgcagcgga cggcgtcgtc
     1681 aaaactttca cggctcgcgt tcggcccaga aatttgccgt attgaggagt ctgtatgaat
     1741 ctccgtcaac ctcacggttc cattttaatt aatttgattg tcatcaccac aatcgttgcg
     1801 gttctgggcg cgggaatgct gtatctgacg acgacttcaa catacgccag tcttttcccg
     1861 aacagccagg cccgcgctta ctacctggcc gaagcgggca ttgcccacgc gcaacagctt
     1921 ctggctcaaa acatccacaa tccttccttt tatcccggag gccacagagg cattgacccg
     1981 ccggtcacct acactttggc caacggcgac cggttcatcc tgaccagcta tgacttcaac
     2041 aacggaggaa cccccgatcc gactcgcgtc gttgtcgaag ccatcggcgt cgtgcatgcc
     2101 ggcacctgga tggaatccaa gaagaaagtg accaagagcg gcatccttcg tgacgacccc
     2161 aatcccgttg acccgacgcc cagcaagaaa accgaagagt tcatcgattt catggtcaac
     2221 gatcaggtgt tcgtttacgg cagcgccatg agcttcagtg gaaataacgt atcgggcgcc
     2281 gatgcaaccg ttgtgattcg cggacccttg gatacggatg ataccaacct gggcgcttct
     2341 attgcagcta cgaccattta catagacgga catgtagaat tgaacaaggg cagcgcaggg
     2401 ctcggatcca gttcttcgcc cggaaatatt tatatcaacg gcgatttaac cctgtggaac
     2461 ggatcacgca gcatctatgg agatgtttac gtgaatggga atcttcgcct gaaggatgcg
     2521 aaaatctacg gaaacattta tgtcaacgga acggtcgaac tgggctggac ccccaccctt
     2581 tccgcaaatg ccaacatcta ctatgtagga gacggcaatc ccgatacgcc cgacatcatt
     2641 catcccaaaa attacgatca atccattctg aacaaatgtc acaaaatacc ctatggagct
     2701 gtgcaggctt ttccgtcctt ctccatgccg gacgtagcca tcccggatcc gcgcggcagc
     2761 gcctggtttg catccaaagg ctatgtgtcg tccggcgcgc cggtaagcgg tttgaaaata
     2821 tataccgaag gcgattacaa ctcatcgacc tggcggccta cggc
//
LOCUS       DAOSIM010000253         2863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_30344_length_2863_cov_7.257479, whole genome shotgun sequence.
ACCESSION   DAOSIM010000253 DAOSIM010000000
VERSION     DAOSIM010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30344_length_2863_cov_7.257479"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1919)
                     /locus_tag="PLR47_09430"
     CDS             complement(84..1919)
                     /locus_tag="PLR47_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOG13210.1"
                     /translation="MNEIMPSYDPPTVMGIFMHQARRFGDRRMSLAKKEGVWQTKTWR
                     QALEALPAAVMGLVSLGLQKGDAVGIISRTRREWSEADMTILTAGGVAIGIYPSASLW
                     EMTHVISHSELQICFVEDEPLLEKLLAVRVKTGLPRTMILFEPSDRPLPPDVMTLESL
                     IAHGRIAYEADPSRFETIWRAVRPEDPATIAYTSGTTGPPKGAVITHANLYFTVMNAT
                     RMHHYDESDFGIAFLPLTHMLQRMTVYAAMHLGIVGAYAENLDKLVDNFRELHPTVQV
                     SVPQIFERIYNRVHQMLDSGSPVKRRIFHWAVAVGRKAALQRGSNRSLPASLALQHAI
                     AHRLVFSKIHDVFGGRVKYLLCGAAPMPLELLEFFDAVGLLILEGYGLTETVAPAAVN
                     RAEHYKFGTVGQLIPGMEAKVAGDGELLLRGRGLFQGYYKDPEATAAAIDAEGWFHTG
                     DIGVIDDEGFLSITGRKKDLIVTAGGKNIAPQNIENLIRSMPLISQAMACGDRRKYLT
                     ALITLNEQELIEFAGRKTIEERDPGVLAGHPAVFRAVDEHVQAVNRRLAPHEQIRRFT
                     ILPRDFTEEAGELTPTLKIRRQEIVRRYGNEIELLYSPKGGESGR"
     gene            2068..>2863
                     /locus_tag="PLR47_09435"
     CDS             2068..>2863
                     /locus_tag="PLR47_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13211.1"
                     /translation="MRISNNDDGIYRQLQIQLDRYPIGYPATKSGVEIDLLKYFFTPL
                     EAKIALCLTLRSISLTPIRRRLKGQFHIDLSPEEMSALLDKMFQKGVIRRSESAGSFH
                     YGIAMLAIGMFEYHVDDLTPELVKMMHQYFDEAFSAEFFRSSLPQLRTSPHLKALVPE
                     YLVEPYDNMRRFIQHTRETIHVANCVCKQGEALLGRPCKQTDHYEVCLLIGKTGYAQR
                     GRAREISKDECLKILDMAEERGMVLQPGNSRKPACICICCGCCCGVL"
BASE COUNT          629 a          857 c          756 g          621 t
ORIGIN      
        1 gcgggcgcct ttgccaagca gcttttccct gggccggtcg tcttccggcc agtccttgat
       61 tcccgaggat tgaagttttt cgctcatctt ccgctttccc ctcctttggg tgaatagaga
      121 agctcgattt cgttcccgta gcggcgaaca atctcctgtc ggcggatctt cagcgtcggc
      181 gtcaattcac ccgcctcctc cgtgaaatcc ctgggaagaa tggtgaagcg cctgatctgt
      241 tcgtgcggag ccaggcggcg gttgaccgcc tgcacatgct catccacggc ccggaacacc
      301 gccggatggc cggccagcac gccgggatca cgttcttcga tggttttccg gccggcaaac
      361 tcgatcagct cctgctcatt cagggtgatc agcgccgtga gatatttccg gcgatcgccg
      421 cacgccatcg cctgactgat gagcggcatc gaccggatca ggttctcgat gttctgcgga
      481 gcaatgttct tgccgcccgc cgtaacgatc aggtctttct tccggccggt aatggaaaga
      541 aatccctcgt cgtcgataac gccgatgtcg cccgtgtgaa accatccttc ggcgtcgatg
      601 gcggcggccg ttgcttccgg atccttgtag tagccctgaa aaaggcccct tccccgaagg
      661 agcagctcgc catcgccggc cacttttgcc tccatacccg gaatcaactg ccccacggtt
      721 ccgaatttat aatgttccgc gcgattgacc gccgccggcg ccacggtttc cgtcaacccg
      781 taaccctcaa gaatcaaaag ccccaccgca tcgaaaaatt cgagaagctc aagcggcatg
      841 ggcgccgctc cgcaaagaag atatttgacc cggccgccga aaacatcatg aattttcgaa
      901 aaaaccagac gatgggcgat agcgtgctgc agcgccagtg aagccggcaa ggagcggttg
      961 ctcccgcgtt gaagagcggc tttccggccc acggccacgg cccagtgaaa aatgcggcgc
     1021 tttacaggcg atcccgaatc cagcatctgg tgaacccggt tgtagatacg ctcaaaaatc
     1081 tgaggcacgc tgacctgaac cgtcggatga agctcacgaa aattgtccac aagcttatcc
     1141 aggttttcgg cgtatgcccc gacgattcca aggtgcatcg ccgcatagac ggtcattctc
     1201 tgaagcatat gggtcagagg cagaaaggcg atgccgaaat cgctttcatc gtaatggtgc
     1261 attcgggtcg cgttcatcac ggtgaaataa agattggcgt gcgtaatcac ggcccccttc
     1321 ggcggtcccg tcgttccgga ggtgtaggcg atcgtcgccg gatcttccgg ccggacagcc
     1381 cgccagatcg tctcaaaacg gctcgggtcg gcttcataag cgatccggcc gtgtgcgatc
     1441 aagctttcca gcgtcatgac atcgggggga agcgggcggt ccgatggttc gaaaagaatc
     1501 atggtccggg gcagacccgt tttcacccga acagccagaa gcttctccag aagcggctcg
     1561 tcttcgacaa aacagatttg caattcactg tgagaaatga catgcgtcat ctcccagagg
     1621 ctggccgacg gataaatgcc gatcgcaacc ccgccggcgg tcagaatggt catgtcggct
     1681 tcgctccatt ccctgcgcgt gcggctgatg attccaacag catcgccctt ttgtaatccc
     1741 agggaaacga gtcccataac ggccgccggc agagcctcca gcgcctgccg ccacgtcttg
     1801 gtttgccaga caccctcttt tttggccaga gacattcttc gatcgccgaa gcggcgcgcc
     1861 tgatgcatga agatccccat cacggtcggg ggatcatatg acggcatgat ctcgttcaaa
     1921 aacagatcct ttgtcttttt gcaatttttt gttgctggaa tataacctta tgacccgcga
     1981 tcgtcaatca acatctttgc cgggggtaca tttgcttgac aaaatcattc tttccctcga
     2041 tgatagaatc caaattcagg gagcatcatg cgcatatcaa acaacgacga tgggatctac
     2101 cggcaactgc aaattcaact ggaccggtat ccgatcgggt atcccgcgac gaaaagcggc
     2161 gtggaaatcg atcttctgaa atatttcttt acgccgctgg aagcaaagat cgccctgtgt
     2221 ctgacgcttc gttccatttc gctgacaccc atccgcagga gactgaaagg ccaatttcac
     2281 atcgaccttt cccccgaaga gatgtctgcc ctgctggaca aaatgtttca aaaaggcgtc
     2341 atccgccgtt cggaaagcgc cggttccttt cactacggca ttgccatgct ggccatcggc
     2401 atgtttgaat accacgtcga tgatcttacc cctgaactcg taaaaatgat gcaccaatat
     2461 tttgacgaag ccttcagcgc ggaattcttc cggtcctccc tgccccaact tcgcaccagc
     2521 ccgcatctga aggccctggt tccggaatat cttgtcgaac cctacgacaa catgcgccgc
     2581 ttcatccaac atacccgcga gacaattcac gtggccaact gcgtctgcaa acagggagag
     2641 gcgctgctgg gaaggccctg caaacagacg gaccattatg aagtttgtct gctcatcggg
     2701 aaaaccggct acgcccagcg cgggcgggcc agagaaatct ccaaggacga atgtctgaag
     2761 atcctggaca tggcggaaga aagaggaatg gtgctgcagc cgggcaattc ccgaaagccc
     2821 gcctgcatct gcatttgctg cggctgctgc tgcggcgtgc tga
//
LOCUS       DAOSIM010000254         2856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_30452_length_2856_cov_7.727954, whole genome shotgun sequence.
ACCESSION   DAOSIM010000254 DAOSIM010000000
VERSION     DAOSIM010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30452_length_2856_cov_7.727954"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            293..604
                     /locus_tag="PLR47_09440"
     CDS             293..604
                     /locus_tag="PLR47_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13212.1"
                     /translation="MSKIIVTDIEQIINQDKRLFWDKTGINSESDKYVIAERILEWGT
                     EKEVRTVLSCYGKDFVRRVVTESRNLSPKTVNYFSVIFGIPRESTRCFSDASRPIWRP
                     Y"
     gene            589..816
                     /locus_tag="PLR47_09445"
     CDS             589..816
                     /locus_tag="PLR47_09445"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOG13213.1"
                     /translation="MAAILKNPVLQELPAQTYLAGGTAAALHLGHRLSVDFDFFTPQE
                     IDSLQWHQRLQSAYGADFTLSAEKIEKIRWC"
     gene            897..1220
                     /locus_tag="PLR47_09450"
     CDS             897..1220
                     /locus_tag="PLR47_09450"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOG13214.1"
                     /translation="MPVAPLADLACMKLIAVNQRGACKDFIDLKFIMEHERILLSFLL
                     QAVGRKYNVGEEMFFQLKKSLVYFDDAERDLNVNMFAPKSGKFERLSPGEWLKTKQFF
                     YSTVG"
     gene            complement(1281..1724)
                     /locus_tag="PLR47_09455"
     CDS             complement(1281..1724)
                     /locus_tag="PLR47_09455"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOG13215.1"
                     /translation="MDEKVRKAIFKRMEQEPFGRKFGITLVDIKEGYARVEMRFTSDM
                     ENMFGIAHGGAIFSLMDAAFEVASNSHGTMAVALNMSVNYLASPAKGALINAEASEIN
                     KTKRTAAYDIRATDESGKLLAACQALVYRLDKPLPFLEDKDRLSP"
     gene            complement(1941..>2856)
                     /locus_tag="PLR47_09460"
     CDS             complement(1941..>2856)
                     /locus_tag="PLR47_09460"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOG13216.1"
                     /translation="NGVKELVVLGMLLAARNVAEGLSWAKGLVGEGDKVPDLIEKGKA
                     KFVGTEILGKKLGVVGLGAIGTMIANTAEDLGMEVMGYDPFLSIEAAWKLSRNIKKAP
                     SLDRLLSESDYITLHVPQNKDTKGFMNAEKFAIMKKGVVLLNFARGGLVETAALKKAI
                     ADGVVSRYVTDFPDEEVIKTDKVIAIPHLGASTEESEENCAIMATMQIMDFLENGNIK
                     NSVNFPECILDRSGKTRLTISNKNEPGMIEKITHLLADNKLNIADMINKSRGSIAYNI
                     IDVEGEISAADLIKKFESVNGIIGVRVL"
BASE COUNT          654 a          720 c          746 g          736 t
ORIGIN      
        1 gacagggcgg gatctatgtc ggagagcgcc aggtgaagtc tccggatgaa aagctttcag
       61 cgggcgactt tgccggcggc cgagaactgc gcatccgcaa gggcaagaaa aaatatctga
      121 tgatagaaat aggggattag actgattccc ctgcgggtga acgcgcgacc taaagctgaa
      181 gaatagagga tagaggcgaa aggccatagg cgaggggata aggcttgaac ggtaatccct
      241 ttcattgcaa aatgacggat attaatgtaa tttattgcaa acaaaattcc ttgtgagcaa
      301 aatcatcgtg accgatatcg agcaaattat aaatcaggat aaaaggcttt tctgggataa
      361 aaccggaatc aattccgaaa gcgacaaata tgtaatcgcc gaacgtattc tcgaatgggg
      421 aacggaaaaa gaggtccgga cggttctttc ctgctacggc aaagactttg tgaggcgcgt
      481 cgtcacggaa agcaggaacc tcagtcctaa aacggtcaat tacttttcag tgatcttcgg
      541 cattccgcgg gagtcgacgc gatgtttttc cgatgcatca cggccgatat ggcggccata
      601 ttaaaaaatc cggttttgca agaattgccc gcgcaaactt atcttgcggg cggcacggcc
      661 gcggctttac atttggggca ccgtctatcc gttgattttg atttttttac tccccaggag
      721 attgacagtc tgcagtggca tcagcgcctg caaagcgctt atggcgctga ttttacatta
      781 tccgccgaaa aaattgaaaa aatacgctgg tgttgaattt aaacacaacc ggattcagca
      841 tttttcgcta tccctattcc ttgcttcatg aacccatttg tgatgccgga ttgcccatgc
      901 ccgttgcgcc gcttgcggat ctggcatgca tgaaacttat agccgtaaat cagcgcggcg
      961 cctgtaaaga ttttattgac ctcaaattta ttatggaaca cgaaaggatt ttactgtcct
     1021 ttctattgca ggccgtcggc cgaaaataca acgttgggga agaaatgttt tttcaactta
     1081 aaaaaagcct cgtttatttt gatgatgcgg agcgtgatct aaatgtcaac atgttcgcgc
     1141 cgaaatcggg aaaattcgag cgcctttcac cgggagagtg gttaaaaacg aagcagtttt
     1201 tttacagcac tgtggggtga agactatttc cgaaattcct tcctgaattc gtccgaattc
     1261 caaaccgaag cttatgaact ttatggtgag aggcgatctt tatcctcaag gaagggcagc
     1321 ggtttatcca ggcgatacac cagcgcctgg caggcagcca gaagctttcc cgactcgtct
     1381 gtggcgcgga tatcgtaagc ggcggttctt ttggttttat tgatttcact ggcttcggcg
     1441 tttatcagcg cgcctttggc gggggaggcc aggtaattga cgctcatgtt gagggcgacg
     1501 gccatggtcc catgcgaatt ggatgcgact tcaaacgccg cgtccatcag cgagaagatc
     1561 gcgccgccat gcgctatgcc gaacatgttt tccatatcgg acgtaaatcg catttccacg
     1621 cgggcgtagc cttcttttat atccaccagg gtgatgccga atttcctgcc gaatggctcc
     1681 tgctccatcc gtttgaagat tgcttttctg actttttcat ccatccctgt tctcctgcgg
     1741 cattatccgg gtcggccggt ttggctatgt cttttaacag gttggcaggc aaagggcaac
     1801 caaaaccccg tgtctccacg ggaagctgcc ccgggcacgt ggagcccaac tgcttgcgga
     1861 ggactgcccg atgcgtttgt caaaaaaaag cgccaagctt gaagccaggc gctttttgta
     1921 gaatatcttt caggtgttga ttaaagaact ctgacgccga tgatcccatt gacggactca
     1981 aatttcttaa tgagatccgc ggcgctgatt tcgccttcca cgtcgatgat gttataggcg
     2041 atgctgcctc tgcttttgtt gatcatgtcg gcaatgttca gcttgttgtc ggccagcaga
     2101 tgcgtgattt tctcgatcat gccgggctcg ttcttgttgg aaatggtgag ccgggtcttg
     2161 ccggaccggt cgaggatgca ctccgggaag ttgacggagt ttttgatgtt tccgttctcc
     2221 aggaaatcca taatctgcat ggtggccata atggcgcagt tttcctcgga ttcctcggtg
     2281 gacgcgccca gatgcggaat ggcgatgacc ttgtccgttt tgatgacctc ttcatccggg
     2341 aagtccgtca cgtagcggct cacgacaccg tcggcaatgg cttttttcag ggccgcggtc
     2401 tccacgagtc cgcctctggc gaagttcagc aaaaccacgc ccttcttcat gatcgcaaat
     2461 ttctcagcgt tcatgaagcc cttggtatcc ttgttctgcg gaacgtgcag cgtgatgtag
     2521 tcggattccg aaagcagtcg atccaggctc ggcgccttct tgatattgcg ggacagcttc
     2581 caggccgcct cgatggacaa aaacggatcg taccccatga cttccatgcc cagatcctcg
     2641 gccgtgttgg cgatcatggt gccgatggcg cccaggccca ccacgccgag ttttttgccc
     2701 aggatctccg tgccgacaaa cttggctttc cctttttcga tcaggtcggg aaccttgtcg
     2761 ccttcaccca ccagcccttt ggcccaggac aggccttccg cgacgttgcg cgccgcaagc
     2821 agcatgccca aaacgaccag ttccttcacg ccgttg
//
LOCUS       DAOSIM010000255         2844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_30621_length_2844_cov_8.864826, whole genome shotgun sequence.
ACCESSION   DAOSIM010000255 DAOSIM010000000
VERSION     DAOSIM010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30621_length_2844_cov_8.864826"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>434)
                     /locus_tag="PLR47_09465"
     CDS             complement(<1..>434)
                     /locus_tag="PLR47_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HOG13217.1"
                     /translation="FVFYNTPGAEAPAEMTFVIPEKKAFCGAEILSQTMHNLLPVRGA
                     KVRNALLWAKYLDDALTYAAGCEVYFGQHNWPIRGRDRIKEFITMHRDVYRYTHDQTV
                     RLINAGYTPREIADAIQLPKALESYWGARGYYGDLRHNVKAV"
     assembly_gap    435..444
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<445..1179)
                     /locus_tag="PLR47_09470"
     CDS             complement(<445..1179)
                     /locus_tag="PLR47_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG13218.1"
                     /translation="MKRIAKQIGPAALCLAAIFLLTACEKDSGVGGVAGDAGEAAIAA
                     NQTMAQKLDFNRDQDFQEALRGLIAKPQGRILAADGTVLVDLGAFHFVAGEAPPTVNP
                     SLWRHAKLNAGIGLFKVVDGIYQLRGFDLGNMTLIEGKTGWIVVDPLTCRESAAFAFA
                     FAQKHLGEKPVSAMIITHSHVDHFGGALGIVSPREAASRRLPVIAPQGFLEEAISENL
                     MVGTAMARRSSYQFGKDLERSAKGMVD"
     gene            complement(1320..>1735)
                     /locus_tag="PLR47_09475"
     CDS             complement(1320..>1735)
                     /locus_tag="PLR47_09475"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13219.1"
                     /translation="FTLVFFASMFVLMESVWQSGFFQNLLSAFSLNMTGIPVIMILSV
                     GVSQLISNVPMAALFLPLMLSGGAGAKELMALAAGSTIAGNLFILGAASNVIIIQHAE
                     KENRGESISFLEFAKAGIPLTILQTAVYLLFFKMN"
     assembly_gap    1736..1768
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1769..2558)
                     /locus_tag="PLR47_09480"
     CDS             complement(<1769..2558)
                     /locus_tag="PLR47_09480"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOG13220.1"
                     /translation="MNLSILILAVVFLLIAIRQIGSARLQIWQIMLGGAVLVILAGEI
                     SPRAAFHAIHFDVIFFLFGMFIIGQAMEDSGYLTHICHRLFGRAKSVDALVLAILFTM
                     GIGSAVLMNDTLAIIGTPIMLLLARRNIISPKLLLLCLAFAITTGSVMSPIGNPQNLL
                     IALGGKIENPFLTFFQYLFAPTIVNLFMAYIVLKVLFKKEFHRTQLEPGIEDIRHIRL
                     AIVARLSLILVVCLILAKVALAVMNLFAPVPFDFKLTAIALIGCS"
     gene            complement(2577..>2844)
                     /locus_tag="PLR47_09485"
     CDS             complement(2577..>2844)
                     /locus_tag="PLR47_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOG13221.1"
                     /translation="LRDFGDKLRDRLASGVILLGSKAEGKALLLCMVTKDIAGKYHAG
                     NIIKELAPLVGGKGGGRPDMAQAGGTKPENLDQVFPALEKLLTA"
BASE COUNT          632 a          755 c          836 g          578 t
ORIGIN      
        1 acacggcctt gacgttgtgc cgcagatcgc cgtagtagcc gcgcgccccc cagtaagatt
       61 ccagagcctt cggcaattgg atggcgtcgg caatttcccg gggagtataa ccggcgttga
      121 tgaggcgcac ggtttggtca tgggtgtagc ggtaaacgtc gcggtgcatg gtgatgaatt
      181 ctttgattcg atccctgccc cgaatcggcc agttgtgctg gccgaaatac acttcgcaac
      241 cggcggcata tgtaagcgcg tcgtcaagat atttggccca gagcagcgca tttctcacct
      301 ttgccccgcg aaccggcagc aggttgtgca tggtctggga aaggatctcc gcgccgcaaa
      361 acgccttctt ttcgggaatg acaaacgtca tttcagcggg ggcttccgcg ccgggcgtat
      421 tgtaaaagac gaagnnnnnn nnnnatcgac cattcctttg gcggagcgtt ccagatcttt
      481 tccgaactgg taggaggagc gccttgccat cgccgtgccc accatcagat tttcgctgat
      541 ggcttcttcc agaaatccct gaggcgcaat gaccggaagg cggcgcgatg ccgcctcccg
      601 cggcgaaaca atacccaaag cgccgccgaa gtgatccaca tgactgtggg tgataatcat
      661 tgccgaaacc ggcttttcgc ccaggtgctt ttgggcaaag gcgaaggcaa aggccgcgct
      721 ttcccggcag gtgagcggat cgaccacgat ccagccggtt ttgccctcga tcagcgtcat
      781 gttgccgaga tcaaagccgc gcagctggta gatgccgtcg acgactttga agagtccgat
      841 cccggcgttg agcttcgcgt gacgccacag gcttgggttg accgtcggcg gggcctcgcc
      901 cgcaacaaaa tgaaaggcgc ctaagtccac caggaccgtt ccgtccgccg cgaggatccg
      961 cccctggggt tttgcgatca agccgcgcag agcctcctga aagtcctgat cccggttgaa
     1021 gtcgagtttt tgcgccatgg tttggttcgc cgctatggcc gcctcgccgg cgtcaccggc
     1081 taccccgccg actccgctgt ccttctcgca ggcggtaagc agaaatattg ccgccagaca
     1141 cagagccgcg ggccctattt gcttcgcgat ccttttcatg gtgttctcct gaagttttcc
     1201 gtctgtgaaa aaacattctg gacgggaaaa tttttcgact ttgtcccgca cgcgcaaaag
     1261 gctgaacttt gtgaatacag gtaatcgcac cgcggaactt ctgtcaatgg tttttcggtt
     1321 cagttcatct tgaagaaaag caggtaaaca gccgtctgga ggatcgtcaa cgggattccg
     1381 gcttttgcaa attcgagaaa ggaaatgctt tcgccgcggt tttccttctc cgcgtgctga
     1441 atgatgatga cgttgctggc cgcgccgaga atgaaaagat tgcccgcgat ggtggagccg
     1501 gccgcaagcg ccatgagctc tttcgcgccg gcgccgccgg aaagcatgag cggcagaaaa
     1561 agcgccgcca tgggcacgtt ggagatcagc tgactgaccc cgacggagag gatcataatg
     1621 acggggatgc cggtcatgtt cagcgaaaag gccgaaagca gattttgaaa aaaaccggat
     1681 tgccaaacgc tttccatcag aacaaacatg gacgcgaaga agaccaaggt aaaccnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnngc gaacagccga tcagggcaat ggcggtcagc
     1801 ttgaagtcaa agggcacggg cgcaaaaaga ttcatcacgg caagagctac tttagccaga
     1861 atcaggcaaa ccacaaggat cagggaaaga cgcgcgacta tggccagccg gatgtgccga
     1921 atgtcctcga tgccgggctc cagctgtgtg cggtgaaact cttttttgaa aagaaccttc
     1981 aggacgatgt aggccatgaa aagattgacg atcgtcggcg cgaacagata ctggaagaac
     2041 gttagaaagg gattttcaat ttttcctccc agggcaatca gcagattttg aggattgccg
     2101 atggggctca tgacgcttcc cgtggtgatg gcaaacgcca ggcaaagcag aagcagtttt
     2161 ggggaaataa tgtttcttcg ggccaagagc agcatgatgg gagtgccgat gatggcaagg
     2221 gtgtcgttca tgaggacagc ggatccgatt cccatggtga aaaggatggc cagcaccagc
     2281 gcatcgacgg actttgcccg gccgaacagc ctatggcaga tgtgggtgag atacccggag
     2341 tcttccatcg cctgcccgat gataaacatg ccgaagagaa agaaaatgac gtcaaaatga
     2401 atggcgtgaa aggctgcgcg cggcgatatt tctcccgcaa ggatcacgag gacggcgccg
     2461 ccgagcatga tctgccagat ctgcagcctg gcgctgccga tttgccgtat ggcgatcagg
     2521 agaaaaacga cggcaaggat caggatggaa aggttcatgc ggggccgccg gtttttctat
     2581 gcggtgagca gcttttccag cgcggggaat acttgatcca gattttccgg ctttgtgccg
     2641 ccggcctggg ccatgtcggg ccggcctccg cccttgccgc cgaccagcgg ggcgagttcc
     2701 ttgatgatgt ttccggcatg atacttcccg gcgatatctt tggtaaccat gcacaaaagg
     2761 agcgccttgc cttcggcttt gctccccaga agaatgacgc cggaggcgag ccggtcgcgc
     2821 agtttgtcgc cgaaatcgcg cagg
//
LOCUS       DAOSIM010000256         2832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_30803_length_2832_cov_8.625135, whole genome shotgun sequence.
ACCESSION   DAOSIM010000256 DAOSIM010000000
VERSION     DAOSIM010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30803_length_2832_cov_8.625135"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PLR47_09490"
     CDS             <1..331
                     /locus_tag="PLR47_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOG13222.1"
                     /translation="LILVSAGYDIYYQDPLGGMRVTPDGFAAMARVLLNIADRCCSGR
                     VAGVLEGGYHTVGLARSAKATLEEMFDETHWTDRKLDELDEQADDENQPVIQAVIKGM
                     RPYWNVF"
     gene            341..628
                     /gene="gatC"
                     /locus_tag="PLR47_09495"
     CDS             341..628
                     /gene="gatC"
                     /locus_tag="PLR47_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOG13223.1"
                     /translation="MKLSTSDVEYVARLARLEISEAEKDKFTAQLNDILRYIDQLNEL
                     DTAGVAPMSHAIAVTNAFREDKVVESLGTQKALANAPDARGEFFRVPKVIE"
     gene            753..2240
                     /gene="gatA"
                     /locus_tag="PLR47_09500"
     CDS             753..2240
                     /gene="gatA"
                     /locus_tag="PLR47_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOG13224.1"
                     /translation="MPANRIVEAFMELHQLTIHELQEKIRGGGASALQIAESVFSRID
                     AVEDRVHSYIRLMKKEAMAAAVRADEQIKKGDIQPLTGIPVALKDIVCTQGIPTTCGS
                     HILHQFVPPYNATVVEKLAEAGAVFVGKANMDEFAMGSSTETSSFGPTRNPWDLERIP
                     GGSSGGSAAAVAAGECIASIGSDTGGSIRQPAALCGVVGMKPTYGRVSRFGLIAFASS
                     LDQIGPFTRDVEDCAIMMNVLAGYDPKESTSVRAEVPDYRRFIGRDIQGWRIGIPGEY
                     FIEGIDPEVSAAVRKAIETVRQCGASIVEISLPHTRYCVAVYYIIAPAEASSNLARYD
                     GVRYGLRTADARDLAEMYEKTRMQGFGAEVKRRIMIGTYALSAGYYDAYYGKASQVRA
                     LIRRDFDEAFTKCDAILTPTSPTPAFRLGEKTDDPLQMYLSDIFTISANLAGIPGISV
                     PCGFSASGLPIGVQFLTGHFEEGRLLQVASAYEKSAAVEKRRPSL"
     gene            2240..>2832
                     /gene="gatB"
                     /locus_tag="PLR47_09505"
     CDS             2240..>2832
                     /gene="gatB"
                     /locus_tag="PLR47_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOG13225.1"
                     /translation="MDFETIIGLEVHAQLLTDTKIFCNCSTKFGASPNSHACPVCLGM
                     PGVLPVLNKKVVEYAMKMALATNCTINPRNVFARKNYFYPDLPKGYQISQYAYPLAEH
                     GYLMIETGERPRKIGITRIHMEEDAGKLIHDDNNPASYVDLNRTGVPLIEIVGEPDIR
                     SPEEAAEYLRRLHEILVYLEICDGNMEEGSFRCDANVS"
BASE COUNT          606 a          796 c          864 g          566 t
ORIGIN      
        1 gttgattctg gtctccgcgg gctacgacat ttactatcag gatccgctgg gcggcatgcg
       61 cgtcacgccc gatgggtttg cggcgatggc acgcgtattg ctcaatattg ccgatcggtg
      121 ctgttcgggg cgtgtggccg gcgttctgga aggcgggtat cataccgtgg ggctggcccg
      181 gtcggccaag gcgacgctgg aagagatgtt tgacgagacg cattggacgg accggaaact
      241 ggatgagctc gacgagcagg cagacgatga aaatcagccg gttattcaag cggtgatcaa
      301 gggcatgcgg ccttactgga atgtgtttta ggaggatgtt ttgaagctca gcacgtcgga
      361 cgtcgaatac gtcgccagac tggcgcgtct ggaaatatcg gaggcggaaa aggacaagtt
      421 taccgcccag ctcaatgata ttctgcgcta catcgatcag ttaaacgagc tggacacggc
      481 gggcgtggcg ccgatgagcc acgcgattgc cgtgaccaac gcgtttcgtg aagacaaggt
      541 tgtggaatcg cttggcaccc aaaaggcgct ggccaacgcc ccggacgcac gcggcgagtt
      601 tttcagagtc cccaaagtga tcgaatgatg cgcccggggg aaagttctgc cgcccgcgcg
      661 aatgattgga accgattttc cagtcgttgc ccggcaggaa accattttct gatacacaaa
      721 cgggcaatcc gggctgaaaa aagcacggat ttgtgcccgc aaacaggatt gttgaggcgt
      781 ttatggagct gcaccagttg accattcatg aattgcagga aaaaatccga ggcggcggcg
      841 cttcggccct gcagattgcc gaatccgttt tttcgcgcat cgacgcggtg gaagaccgcg
      901 tccattccta tatccgcctg atgaagaaag aagcgatggc cgccgcggta cgggcggacg
      961 agcaaattaa aaaaggcgac attcagcctt tgaccggcat tcccgtcgct ctcaaggaca
     1021 ttgtctgcac tcaaggcatt cccaccacgt gcggctcgca cattctgcat caatttgtgc
     1081 cgccctacaa cgcgacggtt gtggaaaagc tggcggaggc cggggccgtg tttgtgggca
     1141 aggccaacat ggatgaattt gccatggggt cctccacgga aacctcgtct tttggcccca
     1201 cgcgaaatcc ctgggacctg gaaagaattc cgggcgggtc cagcggcggt tcggccgcgg
     1261 cggtggccgc cggcgaatgc atcgcctcca tcggctccga tacgggcggc tcgatccgtc
     1321 agccggcggc gctctgcggc gtggtcggga tgaagccgac ctacgggcgc gtttcccgat
     1381 tcggcctgat cgccttcgcc tcttcgctcg atcagatcgg cccgtttacc agagatgtgg
     1441 aagattgcgc gatcatgatg aacgttctgg cgggctatga cccgaaagaa tccacgtcgg
     1501 tccgggcgga agttcccgac taccgccggt ttatcggccg cgacattcaa ggctggagaa
     1561 tcggcattcc cggagaatat ttcatagaag gaatcgaccc ggaagtgtcg gccgccgtcc
     1621 ggaaagcgat tgaaacggtt cggcagtgcg gcgcttcgat tgtggagatt tcgcttccgc
     1681 acacccggta ctgcgtggcg gtttactaca tcatcgcgcc ggcggaggcc tcctccaacc
     1741 tggcgcgcta cgacggcgtc cggtatggtt tgcggacggc ggacgcgcgc gaccttgccg
     1801 aaatgtatga gaaaacccgg atgcagggct tcggcgcgga agtgaagcgc cggatcatga
     1861 tcggcaccta cgccctttcc gccggatact acgacgccta ctacggaaaa gcctcgcagg
     1921 tgcgggcgct gatccgccgg gactttgacg aggccttcac gaaatgtgac gcgatcctga
     1981 cgcccacgtc gccgacaccc gcgtttaggc tgggagagaa aacggacgac ccgcttcaaa
     2041 tgtatctttc cgatattttt actatttcgg ccaatctcgc gggaattcca ggcatctccg
     2101 ttccctgcgg cttcagcgcg tccggtctgc cgataggcgt ccagtttctg accggccatt
     2161 tcgaggaggg gaggctgttg caggtggctt cggcttatga aaaaagcgcc gccgtggaaa
     2221 aaaggaggcc ttctttataa tggattttga aaccatcatc gggctggaag ttcacgccca
     2281 actgctcacg gacacgaaaa ttttctgcaa ctgttccacc aagttcggag cgtcgcccaa
     2341 cagccacgcc tgcccggttt gcctgggcat gccgggcgtt ctgccggtgc tcaacaagaa
     2401 agtcgtggaa tacgccatga agatggcgct ggcgacgaac tgcaccatca atccccgaaa
     2461 cgtcttcgca aggaagaatt atttttatcc cgacctgccc aagggctacc agatttcgca
     2521 gtacgcctat ccgctcgcgg agcacgggta cctcatgatc gagacgggcg agaggccccg
     2581 gaagatcggc atcacaagga ttcacatgga ggaggacgcg ggcaagctga tccatgacga
     2641 caataacccg gccagttacg tggatctgaa ccgcaccggc gtgccgctca ttgaaatcgt
     2701 cggcgaaccg gacatccgct cacccgaaga ggcggcggaa tacctccggc gactgcatga
     2761 aattctagtg tatctggaaa tctgcgacgg caacatggaa gaaggctctt tccgctgcga
     2821 cgccaacgtg tc
//
LOCUS       DAOSIM010000257         2822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_30941_length_2822_cov_9.091073, whole genome shotgun sequence.
ACCESSION   DAOSIM010000257 DAOSIM010000000
VERSION     DAOSIM010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30941_length_2822_cov_9.091073"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PLR47_09510"
     CDS             <1..924
                     /locus_tag="PLR47_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG13226.1"
                     /translation="PVTPLVAIGGALYIMYGMVLSQPVNAAVSLVLTLAGIPVLYGMT
                     RGLPRFEGFAVSKKYLVLGGTALILAVLLITPKFIDTRPVLRVAVEISNPPIAFEDET
                     GKPAGLSIDTIQAVAGEMGYRIAFRPTAFEHIPLALEKDLVDVAVGELTVTPERSRRA
                     AFSRSYRQGTLALLVRPESPAAALEDLRGKEIGVKDRSTGDGYMKQLSGYRLLRLDVS
                     SDLLELFNEGALDAVVFDKPIIDEWLARGLAKGRSIPLGEQEQYAIAYRKENARLGRE
                     IDRALDKLIKSGHMEELQKKWLSGRQERPSS"
     gene            921..>1280
                     /locus_tag="PLR47_09515"
     CDS             921..>1280
                     /locus_tag="PLR47_09515"
                     /inference="COORDINATES: protein motif:HMM:NF015286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HOG13227.1"
                     /translation="MNVIATSAFDIFKIGPGPSSSHTLGPMKAAGCFREACAGLKKAA
                     LEKATAIEVSLYGSLATTGKGHGTDRSVIAGLLGWNPETVDPDAFDPLGTDSKMVYTF
                     CAGKKEIPFRAGDIHFDT"
     assembly_gap    1281..1318
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1319..2265
                     /locus_tag="PLR47_09520"
     CDS             <1319..2265
                     /locus_tag="PLR47_09520"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOG13228.1"
                     /translation="ILLEQEYYSVGGGFILCKGEAERPRPIPPVPYASMHQLRAQMDR
                     LNIGLVELLRRNEKVVSGLDASQVDTGLDAIIAAMVSAVERGLATDAILPGSIGLARK
                     APVLLHRAQTMPDSPERFIILLNAYALAASEENAAGHRVVTAPTSGSSGIIPGIIYFA
                     LKHLGISRELIREGLLVASAIGGIARHNASISGAEVGCQGEVGVASAMAAAMIAHLRG
                     CQMAVIENAAEIALEHHLGLTCDPIGGYVQIPCIERNAVGAIHAYNAYLLASAGDPTR
                     QKIDFDAVVEAMLETGRDMSERYKETARAGLAVCSIRC"
     gene            complement(2276..>2822)
                     /locus_tag="PLR47_09525"
     CDS             complement(2276..>2822)
                     /locus_tag="PLR47_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOG13229.1"
                     /translation="RFEQTLSLFRKYGSRYGFDPLMLAAQGYQESRLDQSKRSPAGAI
                     GIMQIMPKTGRSLKVGDIRITEPNIHAGAKYLDLLMANYFSDARFSDQDRSLFSFAGY
                     NAGPGKISRMRTLAKERGLDPNRWFNNVELVTAEKVGFETTTYVRNIYKYYIAYKLTI
                     SSMEEKQKILEQAVADPSRKP"
BASE COUNT          512 a          833 c          864 g          575 t
ORIGIN      
        1 cccgtgacgc cgcttgtcgc catcggcggc gccctctaca tcatgtacgg catggtcctg
       61 agccagcccg tgaatgccgc ggtttcgctg gtcctgacgc tggccggaat cccggtcctg
      121 tacggcatga cccgtgggct gccccgcttc gaaggctttg cggtcagcaa aaaatatctc
      181 gttctgggag ggacggcgct tatccttgcc gttttgctga tcaccccgaa gtttatcgac
      241 acccgaccgg tgctgcgggt tgccgttgaa atctccaatc cgcccattgc ctttgaggac
      301 gaaacgggca agccggcggg gcttagcatc gatacgattc aggcggtcgc cggggagatg
      361 gggtatcgga tcgccttccg ccccacagcg ttcgagcata ttcccctggc cctggaaaag
      421 gatctggtcg atgtcgccgt cggcgaactg acggtgaccc cggagcgcag ccggcgggcg
      481 gctttctccc gaagctatcg tcaggggacg ctggccctgc tggtgcgccc ggagagcccg
      541 gccgccgcgc tggaggattt gcggggaaag gagatcggcg tcaaggatcg ttcgaccggc
      601 gatggctaca tgaaacagct ttccggctat cgcctgctca ggctcgacgt ttcctccgat
      661 ctgctggagc ttttcaacga aggcgcgctg gatgccgtcg tcttcgacaa gcccatcatc
      721 gacgagtggc tggcccgggg gctcgccaaa gggcgatcca ttccgctggg tgagcaggag
      781 caatatgcca ttgcctaccg gaaagaaaac gcaaggcttg gccgtgagat cgaccgtgcg
      841 ctggacaaat tgatcaaaag cggccatatg gaagagctgc agaagaaatg gctgagcggc
      901 cgccaggaaa ggccttcctc atgaacgtga ttgcgacatc ggcattcgat atcttcaaaa
      961 tcggtccggg tccttccagc tctcacacgc tggggcccat gaaggcggcc ggctgcttcc
     1021 gcgaagcctg cgccggactg aaaaaagccg ctttggaaaa ggcgacggcc atcgaggtat
     1081 ccctgtacgg gtcgctcgcc accacgggaa aaggacacgg gacggatcgc tccgtaattg
     1141 ccggcctgct gggatggaat ccggagaccg tcgatccgga cgccttcgac ccgcttggca
     1201 cggattcgaa aatggtttat accttctgcg ccggcaaaaa ggagatccct ttccgcgcgg
     1261 gcgatattca tttcgacaca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
     1321 attctcctgg agcaggaata ctactccgtc ggagggggct tcattctctg caagggagag
     1381 gcggagcgtc cgcgtccgat tccgccggtt ccctacgcca gcatgcacca gcttcgcgct
     1441 cagatggacc gtctgaacat tggcctcgtc gaactcctgc ggcgcaacga gaaggtggtt
     1501 tccggtctgg acgccagcca ggtggatacg ggactggatg caatcatcgc cgcgatggta
     1561 agcgccgtcg aacgcggtct ggccacggac gccattctgc ccggatccat cggtctggcc
     1621 cgcaaagcgc cggttcttct gcatcgggcg caaacgatgc ccgattcgcc ggagcgattc
     1681 atcattttgc tcaacgccta tgcgctggcc gcctccgagg aaaacgcggc ggggcatagg
     1741 gtcgtgacgg cgccgacgtc cggctcgtcc ggaatcattc ccggaatcat ttactttgcg
     1801 ttgaagcatc tggggatttc ccgggagctg attcgcgagg ggcttctcgt cgcctcggcc
     1861 atcggcggca ttgcccgcca caacgccagc atctccggag ccgaagtcgg ctgccagggg
     1921 gaagtcggcg tcgcttcggc catggcggcc gccatgatcg cgcatctgcg gggctgccag
     1981 atggccgtca ttgaaaatgc cgcggaaatc gccctggagc atcatctggg actgacctgt
     2041 gaccccatcg gcggttatgt tcagattccc tgcattgaac gcaacgccgt cggcgcgatc
     2101 cacgcctaca atgcctatct tctcgcgtcc gcgggggatc cgacccgcca gaagatcgac
     2161 ttcgacgccg tggtggaagc gatgctggaa acgggccggg atatgtctga gcggtacaag
     2221 gaaacggccc gggccggact ggccgtctgt tccatccgtt gttgatcagg gtccgtcatg
     2281 gtttgcggga gggatccgca acggcctgtt cgaggatttt ttgtttttcc tccatgctgc
     2341 tgatcgtcag cttgtaggca atgtaatatt tgtaaatatt ccggacgtag gtggtggtct
     2401 caaaccccac tttttcggcg gtgaccagct cgacgttatt gaaccaccgg ttcgggtcca
     2461 ggccgcgctc tttcgccagc gttcgcattc tcgagatttt tcccggcccc gcgttgtagc
     2521 cggcaaagga aaaaagcgat cggtcctgat cgctgaagcg ggcatccgaa aagtagttgg
     2581 ccatgagcag gtccagatac ttggcgccgg cgtgaatgtt gggctcggtg attcgaatgt
     2641 ctcccacttt cagcgaccgg cccgttttcg gcatgatctg catgatgccg atcgcgcccg
     2701 cggggctgcg tttgctctgg tccagccggg attcctggta tccctgggcg gccagcatca
     2761 gcggatcgaa tccgtaccgg ctgccgtatt tccgaaacag gctcaaggtt tgttcaaacc
     2821 gc
//
LOCUS       DAOSIM010000258         2808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_31137_length_2808_cov_10.293861, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000258 DAOSIM010000000
VERSION     DAOSIM010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31137_length_2808_cov_10.293861"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLR47_09530"
     CDS             <1..184
                     /locus_tag="PLR47_09530"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOG13230.1"
                     /translation="DEDYRVVRRVEFSRFVLNQDKGSAIRGPGRVDLFCGFGPEAQAT
                     AGSLKEKGELFFLLLK"
     gene            522..752
                     /locus_tag="PLR47_09535"
     CDS             522..752
                     /locus_tag="PLR47_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Y4mF family
                     antitoxin"
                     /protein_id="HOG13231.1"
                     /translation="MKIKAATAANIGAAIRKKRKEDGLTLADAAALCGVGYRFLSDLE
                     NGKPTAQMGKVLQVLTALGLDLYIASREWPDE"
     gene            745..1989
                     /locus_tag="PLR47_09540"
     CDS             745..1989
                     /locus_tag="PLR47_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HOG13232.1"
                     /translation="MNNHIGNPGLDVYLRDQKVGRLWLDEKRRFVFQYDEAWISKKGA
                     IPLSLHLPLRAETYPDDLSRPFFSNLLPEAEIKRIIAQRLQISVNNDFAMLGSIGGEC
                     AGAVSVLPSGIAPAVKPGYRELKEEELHKIIIDLPRRPLMAGVEGMRLSLAGAQNKLP
                     VYMEDDRIFIASGNAPSTHILKPPIRDLEDTVENEAFCMMLAQKMALPAPPVKIRRGL
                     DQLFIIERYDRSRDKSGQIVRMHQEDFCQALGFLPDQKYESEGGPSLKHCFTLLQEKS
                     IRPAADRMTLLRWVIFNFLIGNADAHAKNLAMIFTDRGPRLAPFYDLICTQVYPDLTE
                     KAAMRIGGENRPSWIQQKHWEKLGEAVAIKPGLIVKTWKDMSSGIIPKAQVMVNDFKK
                     SHGSTGIIEKIMAVIEKRAGEV"
     gene            2176..2463
                     /locus_tag="PLR47_09545"
     CDS             2176..2463
                     /locus_tag="PLR47_09545"
                     /inference="COORDINATES: protein motif:HMM:NF039183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HOG13233.1"
                     /translation="MNEILLGLAIALQIGDGVTTCFALQKQGVRERNAILRRLFDRLG
                     VVRGLALAKSFGVAVCWIAYAYGGPFAVYTLGFIVALYAVVVINNLKSLCR"
     gene            complement(2511..>2808)
                     /locus_tag="PLR47_09550"
     CDS             complement(2511..>2808)
                     /locus_tag="PLR47_09550"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG13234.1"
                     /translation="LTKLMTNNLFIGGLPESAYQGSSVTVPAGANLYVFSDGVYEVDP
                     PEGSMWTLDQLGEYLLQHKMEAGEDIDALYLKMQDYHGQKILEDDFSMLAVHFT"
BASE COUNT          682 a          733 c          771 g          622 t
ORIGIN      
        1 ggacgaggat taccgggtgg tccgccgcgt cgaattttcg cgctttgtcc tgaaccagga
       61 caagggatcc gccattcggg gccccgggcg cgtcgatctg ttctgcgggt tcggccccga
      121 agcgcaggcc accgccggca gcctcaagga aaaaggcgaa ctcttctttc tgctgctcaa
      181 ataaattcga tctttactta tcgccactga ccgggcgctt tccattttga cgggacaggc
      241 cgatcattaa aacagatcag aatatcttcc gcataaggat atgcatttgg tctggagcaa
      301 ccatgccggc atctcctaaa gttttcggtt caggtgagat acgtctgaac cggcaaaatc
      361 atatcggatc ataatcatgc ttgcagcaat tccccatcgg gaatattcat gcagcaagaa
      421 gcatattcgc cataaacgat tccctatcgg gaatatattg cttgactgtg gcgttataag
      481 cggttattat tcccgaaaag gaatgacgga gaacaggaaa gttgaagatc aaagcagcca
      541 cggcggcaaa tatcggcgca gccatcagga aaaaacgcaa ggaagacgga ctgactctgg
      601 ccgatgcggc ggctttatgc ggcgtcgggt acagattttt gtctgatctg gaaaacggca
      661 agccgaccgc gcagatggga aaagtcctgc aggtgctgac ggcgctggga ctcgatcttt
      721 acattgcatc cagggagtgg ccggatgaat aatcatatcg gcaatccggg attggatgtc
      781 tatctccggg atcagaaagt tgggcgcctg tggctggatg aaaaaagacg ctttgtgttt
      841 caatatgacg aggcctggat cagcaaaaaa ggcgccatac cattatcttt gcacctcccc
      901 ctgagagctg agacctatcc tgatgatctg tcccgtcctt ttttttccaa tcttctgccg
      961 gaagcggaga tcaaacgaat catcgctcag cgtctgcaaa tttcagtaaa caatgacttt
     1021 gccatgctgg gcagcatcgg cggcgaatgc gccggggcgg tatccgtgct cccctcaggc
     1081 attgcgcctg ctgtgaaacc gggttatcgg gaacttaaag aagaagaact gcacaagatt
     1141 atcatcgacc tgccccgaag gccgttgatg gccggcgtgg aagggatgcg gctttccctg
     1201 gccggagcgc aaaacaagct gccggtttac atggaggacg accgcatctt catcgcctcg
     1261 ggaaatgcgc caagcacgca tattctgaaa ccgccaatca gggatctgga ggatacggta
     1321 gaaaatgaag ctttttgcat gatgctggct caaaagatgg cgctgcccgc tccgccggtt
     1381 aaaatccgcc gggggcttga tcagcttttc atcatcgagc gttatgaccg gagccgcgat
     1441 aagagtggcc aaatcgtgcg catgcaccag gaagactttt gccaggccct tggatttctg
     1501 cccgatcaga aatacgaaag tgaaggcggc ccttctctaa aacactgttt tactctgcta
     1561 caggaaaaaa gcatacgccc cgctgccgac cgcatgaccc tgttgcgatg ggtgatcttc
     1621 aacttcctga tcggcaatgc cgacgcccac gccaagaatc tggccatgat cttcaccgac
     1681 cgggggccgc gcctggcgcc cttttacgat ctgatctgca cgcaggtcta tccggattta
     1741 acggaaaagg cggccatgcg gataggcggc gaaaacaggc cgtcctggat acagcagaaa
     1801 cactgggaga aactgggcga ggccgttgcg atcaagccgg gccttattgt aaaaacatgg
     1861 aaagatatgt ccagcggcat cattccaaag gcgcaagtca tggtgaatga tttcaaaaaa
     1921 tcacacggaa gcacaggaat cattgaaaag ataatggccg tcattgagaa acgggccggt
     1981 gaagtctgaa acaaaggtca ggccaacccg ccgagcctga agaaaaacat gcacagtcct
     2041 ccaacaaaaa ttggggtcgc aagatccggg ttgcccagcg tcggcatatc ggcaaagcga
     2101 tacattccac gtcccagtcg ctcaatgacg ccttgatcgc gcatcttgta gcggatccgg
     2161 gaaggatgga aatcaatgaa tgagatcctt ttaggcctgg ccatagccct gcagatcggc
     2221 gacggagtga cgacctgttt tgcccttcaa aagcagggcg tgagggaacg aaacgccatt
     2281 ctgcgccggc tgtttgacag gctcggcgtt gtccgcggac tggcgctggc caaaagcttc
     2341 ggcgttgcgg tttgctggat tgcctacgcg tacggcgggc cgtttgccgt ttacactctg
     2401 ggcttcatcg ttgctttgta tgcggttgtc gtgatcaata atcttaaatc gctttgccgg
     2461 tgacgtttcc cgccacggtc gcaaagtcct gttgccgcac atccccgatg ctatgtgaaa
     2521 tgaacggcca gcatggaaaa gtcgtcttcc aggattttct ggccgtggta gtcctgcatc
     2581 ttcaggtaca gagcgtcaat gtcttcgccc gcttccattt tgtgctgcag cagatattcc
     2641 cccagttgat ccagcgtcca catgctgcct tcgggaggat ccacttcgta aacgccgtcg
     2701 gaaaagacgt aaagattcgc tccggccgga acagtgacgg aacttccctg atacgcgctt
     2761 tccggaaggc cgccgatgaa caggttgttc gtcatgagtt tggtcagg
//
LOCUS       DAOSIM010000259         2775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_31587_length_2775_cov_9.386397, whole genome shotgun sequence.
ACCESSION   DAOSIM010000259 DAOSIM010000000
VERSION     DAOSIM010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31587_length_2775_cov_9.386397"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PLR47_09555"
     CDS             <1..674
                     /locus_tag="PLR47_09555"
                     /inference="COORDINATES: protein motif:HMM:NF015214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HOG13235.1"
                     /translation="ALLFALFHRHSYSGCKPAIGDVILGTAALAAECNNVHKESHVRE
                     KLAELIMTIELGYAAGYTASDLGKPEVYIPGMGFVPFGPGSYIPHSIYCNVGRCLSGE
                     AVWREAEILCDIAGGVVATFPHEKDFTNPATGEALLKYTKRNPKMSPEDQAQFWRYLG
                     DHLCSATGGIVNVGNYHGGGSPIMEQIAITTQYDIESRKKLVKYIAGMSDGDREVFAP
                     KPDKK"
     gene            814..1617
                     /locus_tag="PLR47_09560"
     CDS             814..1617
                     /locus_tag="PLR47_09560"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOG13236.1"
                     /translation="MFAMFDISQFSLKDKVAVVTGGSRGIGQAIAYGFAKAGAKVVIT
                     SRKAQDLEATACEMTAIGCEVLVVPAHLGKIGEVYRVVDTVLEKFGRIDILVNNAGAS
                     PAMASVLDTDERLWDTLMNLNLKGTYFMSQAAARVMKKQGGGKIINVASIDGFKPEPF
                     VSVYSISKAGVRMITKAFAMELARDNIQVNSICPGPISTKMMNSHWMHLPPEEAKKQK
                     EEMCKHLPTCRIGEPDEIAGAAVYLASEASNYTTGAEIVIDGGLLLGMH"
     gene            1635..>2775
                     /locus_tag="PLR47_09565"
     CDS             1635..>2775
                     /locus_tag="PLR47_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOG13237.1"
                     /translation="MKTLEHLLSPITIRSLTIPNRLVMPPMGTALGNPDNTVSEANLA
                     YIKRRAQSGAGLIITEITEVHPLGSASPRCIGVWDDKFIPGLSRLADAVHARGSKIAM
                     QLHHTGRENYLLQKKNKAVAPSAIPSYIFGFLGTPREMTLAEIEETIAAFGSAAVRAR
                     KAGFDAVELHGAHGYLLMQFLSAHSNQRKDQYGGDFRGRARFMIECLEEVRRQVGPDF
                     PMSIRISGEEYIKNGYTITDMEAILPDLVSAGADIINVSFGTHGSPAVNIDTPNPSAP
                     VEYAPGFKSHLARRVKEGAGVPVISVGRYTDPYFMDEVIARGDADMIAVARQHLADPD
                     FLKNAQAGHPEDTLECLACNQGCIERLSLEALPIRCAINPQTGQEL"
BASE COUNT          596 a          841 c          815 g          523 t
ORIGIN      
        1 tggcgctgct gtttgccctg ttccaccgtc attcctactc cggctgcaag cccgccatcg
       61 gcgacgttat tttgggcacc gcggcgctgg ccgccgaatg caacaacgtc cacaaggaat
      121 cccatgtccg tgagaaactg gccgaactga tcatgacgat tgaactggga tatgccgccg
      181 gctacaccgc ctccgatctg ggcaagccgg aagtctatat tcccggaatg ggttttgtgc
      241 cgtttggccc cggctcctat attccccatt ccatttactg caacgtgggc cgctgcctgt
      301 cgggcgaggc ggtctggcgc gaggcggaga ttctgtgcga catcgcgggc ggcgtcgtgg
      361 cgacgttccc gcatgagaaa gattttacca atccggccac gggcgaggcg ctcctgaagt
      421 acaccaagcg caatccgaag atgtcgccgg aggaccaggc gcagttctgg cgctacctgg
      481 gcgatcatct ctgttcggcc accggcggca ttgtcaacgt cggcaactat cacggcggcg
      541 gctctccgat catggagcag attgccatca ccacgcagta cgatatcgag tcccgcaaga
      601 agctggtgaa atacatcgcc ggcatgagcg acggcgaccg ggaagttttt gcgcccaaac
      661 cggacaagaa ataaccatct tacccgcggg ggtcgcctgc ggggagcggc ttcgcaccgt
      721 tccccgcggc ggacgccccg tatttttcct gatcaaaagg ggttgcaatt tccgctgcat
      781 cttttctgac gcatccaaca acataaaaaa ggagtgtttg ccatgtttga tatttcccag
      841 ttttcattaa aggataaagt ggcggttgtc accggcggca gccgcggcat cggccaggcg
      901 attgcctacg gtttcgccaa agcaggggcc aaagtggtta ttaccagccg aaaggcgcag
      961 gatctggaag ccaccgcctg cgaaatgacg gccatcggct gcgaggtgct ggtggtgccg
     1021 gcgcatctgg gcaaaatcgg cgaagtttat cgggtggtgg acacggttct ggagaaattc
     1081 ggacgaatcg acattctggt caacaacgcc ggcgccagcc ccgccatggc ctccgtcctg
     1141 gacacggacg agcgcctctg ggatacgctg atgaatctca acctcaaagg cacctacttc
     1201 atgagccagg cggcggccag ggtcatgaaa aagcagggcg gcggaaaaat catcaacgtc
     1261 gcctccatcg acgggttcaa gccggagccg tttgtcagcg tgtattccat ttccaaggcg
     1321 ggtgtgcgga tgatcaccaa ggccttcgcc atggaactgg caagggacaa cattcaggtc
     1381 aattccatct gtcccggccc catcagcaca aagatgatga attcccactg gatgcacctg
     1441 ccgccggaag aggcgaaaaa gcagaaggaa gagatgtgca agcatctgcc gacctgccgg
     1501 atcggggaac cggatgaaat cgccggagcc gccgtttacc tggcctcgga agcgtcaaac
     1561 tacacaacgg gcgccgaaat cgttatcgac ggcggattgc tgttagggat gcattaacgc
     1621 gaaggagaac gattatgaag accctcgagc atttgttgtc cccgatcacc atccgttcgc
     1681 tgacgattcc caaccggctg gtcatgcccc ccatgggaac agccctcggc aatcccgaca
     1741 acaccgtcag cgaagccaac ctggcctata tcaaacgcag ggcgcagagc ggcgccggcc
     1801 tcatcatcac ggaaatcacg gaagtgcacc cgctgggttc cgcctccccg cgatgcatcg
     1861 gcgtctggga tgacaaattc attcccggcc tgagcaggct tgccgatgcg gtgcatgcgc
     1921 ggggaagcaa aatcgccatg cagcttcacc atacgggccg cgagaactac ctgctgcaga
     1981 agaaaaacaa ggctgtcgca ccttcggcaa tccccagtta tatcttcggt tttctcggaa
     2041 cgccgcgcga gatgacgctt gccgaaatcg aggaaaccat tgccgctttc ggcagcgcgg
     2101 ccgtgcgggc gcgaaaggcg ggatttgacg ccgttgagct tcacggagcg catggctatc
     2161 tcctcatgca gtttctttcg gcgcacagca atcagcgcaa ggatcagtac ggaggcgatt
     2221 tccgcggacg ggcgcgcttt atgatcgaat gcctcgagga agtccgccgc caggtcggtc
     2281 cggatttccc catgtcgatc cggatctccg gcgaggagta tatcaaaaac ggctacacca
     2341 tcaccgacat ggaagccatc ctccccgatc tggtgagcgc cggcgcggac atcatcaatg
     2401 tttccttcgg cacgcacggc agtcccgccg tgaacatcga tacgccgaac ccgagcgcgc
     2461 cggtcgaata tgcccccggc ttcaagtccc acctggcgcg cagggtgaaa gagggcgcgg
     2521 gcgttccggt gatttccgtc gggcgctaca cggatcctta tttcatggac gaagtcatcg
     2581 cccgcggcga cgcggacatg attgccgtgg cgcgccagca tctggccgat ccggatttcc
     2641 tcaaaaacgc gcaggccgga catcccgagg acacgctgga gtgcctggcc tgcaatcagg
     2701 gctgcattga gcgcctctcc ctggaggcgc tgccgattcg ctgcgccatc aatccgcaaa
     2761 ccggtcagga actga
//
LOCUS       DAOSIM010000260         2764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_31771_length_2764_cov_6.086379, whole genome shotgun sequence.
ACCESSION   DAOSIM010000260 DAOSIM010000000
VERSION     DAOSIM010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31771_length_2764_cov_6.086379"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..947
                     /locus_tag="PLR47_09570"
     CDS             138..947
                     /locus_tag="PLR47_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG13238.1"
                     /translation="MPPKERLLIEWFERYTARYRDAVDLLPAALELKYRHSRRVAENA
                     RLIALGLKLDESEIQLAEICGLVHDIGRFPQYSRYGTFRDADTLDHGLAGRKTLESEG
                     VPSLLDEDEWARVSCAVEYHNRKTDDLPADLPENARQLLNIIRDADKLDIMDLVLHSV
                     ARDGFRELPGMLPHIRLSRELTPSVVEEFQKHRSISTSHLETVADFLLMLAFWFYDFN
                     FFSSRQIAVSRDLIGRLQKELPETPFIRELFAGIRKMTNSEGMEISLPDLK"
     gene            complement(1007..2524)
                     /locus_tag="PLR47_09575"
     CDS             complement(1007..2524)
                     /locus_tag="PLR47_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HOG13239.1"
                     /translation="MSIKLMEKARIHCDRTAIVAPEGVFSYRQLLDASARVAAFLLEG
                     SPDLRERPVAFLTPPGFRYVALQWGIWRAGGIAVPLSVSHPRPELEYVLADTEPAAVL
                     ADSDFVARLQPSAETLSFRFARAVDALEHAPGALPDVAPARRAMILYTSGTTGRPKGV
                     VLTHLNLTAQIESLVKAWEWYAGDHILEFLPLHHIHGIVNVISCALRAGAVCEILPAF
                     DAHAVWERIGRGGLTLFMAVPTIYAKLIDAWRSSSPADRQRFSEGCRRMRLLVSGSAA
                     LPVSILEMWREISGHVLLERYGMTEIGMALSNPLHGMRVPGAVGTPLPGVEVRLVDDA
                     GKPVAPGTPGEIEVRGETVFSEYWRRPEDNRQAFRDGWFQTGDVAVFESGSYRILGRN
                     SVDIIKTGGEKVSALEIESILLTYPAIRECAVVGIPDPIWGEKVCAALVPEAGQEPDV
                     MMLRAWLKERLAPYKAPKEIAILRELPRNAMGKLIKAELKKFFQLSWGRGDDKSC"
BASE COUNT          595 a          826 c          812 g          531 t
ORIGIN      
        1 acggatgttt atcttatcaa aagcggtccc gacgcaggga aaaagatact gtaagagccg
       61 gcccggtgaa aaattttaaa gccccgtctc cgaaaagaga agatgatcct ttcctccttt
      121 cggcacacag gaaaatcatg ccccccaaag aacggcttct gatcgaatgg ttcgaacggt
      181 acacggcgcg ctaccgagac gcggtggatt tgctgcctgc cgcgctggag ctcaagtacc
      241 gccacagccg gcgcgtcgcg gagaatgccc gtctgatcgc cctcggtttg aagctcgacg
      301 agtcggaaat tcagctcgcg gaaatctgcg gactggttca cgacatcgga cggtttccgc
      361 aatacagccg ttacggcact ttccgggacg cggatacact ggaccacggc ctggccggtc
      421 ggaagacgct ggagtccgaa ggcgtgccgt ccctgctgga cgaagatgag tgggcgcgtg
      481 tttcctgcgc cgtcgagtac cacaaccgga aaacggacga tctgcccgcg gaccttcccg
      541 aaaatgcccg gcagttgctg aacatcattc gggacgcgga caaactggat atcatggatc
      601 tggttttgca ttccgtcgcc cgcgacggat ttcgcgagct tcccggcatg ctgccccaca
      661 ttcgcttaag ccgggaactg acgccctccg tcgtggagga atttcaaaaa cacaggtcca
      721 tctctacaag ccatctggaa acggttgcgg actttctgct gatgctggct ttctggtttt
      781 acgatttcaa tttcttttca tcccgacaga tcgcggtttc ccgcgacctg atcggccggc
      841 tgcaaaaaga actgccggag acccctttca tccgggagct ttttgccggc atcaggaaaa
      901 tgacaaattc cgaagggatg gagatttcac tccctgacct gaaatagttt tcaactgcac
      961 atggacgcga ccgatgaatg gatgcagtcg aatttgacag acacgatcaa cagcttttgt
     1021 catctcccct accccaagaa agctggaaga acttcttcag ctcggccttg atgagtttcc
     1081 ccatcgcgtt ccggggcaat tcgcgaagaa tcgcgatctc cttgggagcc ttgtacgggg
     1141 ccaggcgctc cttgagccag gcgcgcagca tcatgacatc cggttcctga ccggcctcgg
     1201 ggacgagggc ggcacagacc ttctcccccc agatcggatc gggaatgccg acgacggcgc
     1261 actcccgaat cgccggatac gtcagcagaa tcgactcgat ctccagcgcg gaaaccttct
     1321 ctcctcccgt cttgatgatg tccacgctgt tgcgtccgag aatgcgatag gatccgcttt
     1381 cgaacaccgc gacgtcgccg gtctggaacc atccgtcgcg gaacgcctgc cggttgtcct
     1441 ccggccggcg ccagtactcg gagaagacgg tctcgccgcg cacctcgatc tctcccgggg
     1501 ttccgggcgc aaccggcttt ccggcatcgt ccacgaggcg cacctcaact cccggcagcg
     1561 gagtgccaac cgcgccgggg acgcgcattc cgtgcagggg attggacagg gccatgccga
     1621 tctccgtcat cccgtatcgt tcgagcagga catgcccgga gatctctctc cacatctcca
     1681 agatcgaaac aggcagggcc gcggaccccg acaccaataa ccgcatgcgt cggcagcctt
     1741 cggagaagcg ctgccggtcg gcgggggacg acgaccgcca ggcgtcgatc agtttggcat
     1801 agatggtcgg caccgccatg aagagcgtca gaccgccccg gccaatgcgt tcccagaccg
     1861 cgtgggcgtc gaatgcgggg aggatctcgc agacggcgcc cgcccgcagc gcgcaggaga
     1921 tgacgttcac aatgccgtga atgtgatgga ggggcaggaa ttccagaata tgatcgcccg
     1981 cgtaccattc ccaggccttg accaggcttt cgatctgggc cgtgaggttg aggtgagtca
     2041 aaaccacgcc cttcggcctg ccggtggtgc cgctcgtgta gagaatcatc gcccggcgcg
     2101 cgggggcaac gtcgggcaag gcaccgggcg cgtgttcaag ggcatcaacg gcccgggcga
     2161 atcggaaact cagggtttcg gcggagggct gcagacgcgc gacgaaatcg gaatcggcga
     2221 gaaccgccgc gggttcggta tcggcaagca cgtattccag ttccggccgt ggatgggaaa
     2281 cggacagcgg caccgcaatg ccgccggcgc gccagatgcc ccactggagc gccacatagc
     2341 ggaatcccgg aggcgtcagg aaggcgacgg gccgctcccg caggtccggc gatccctcca
     2401 gaagaaaggc ggcgacgcgg gccgaggcat ccagcaactg ccgataggaa aacacacctt
     2461 ccggggcgac aatcgccgtc cgatcgcagt ggatacgggc cttttccatg agcttgatcg
     2521 acatcgtgcc tccgtggacg gtcgttgggt tagatgacgg gtgacggaat cggtcagagg
     2581 attggggtca cccattttat tctggttcag taatccttaa atgattttca gcaaacgtac
     2641 ataaaggatt tccaccatgg cgttttgatg aagaagcagt gttcaatagt caaaatttat
     2701 atacaaatac actactgtcc ggaataatcc gtaaataagt tcaactggtt actgttttcg
     2761 acca
//
LOCUS       DAOSIM010000261         2750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_31971_length_2750_cov_7.968089, whole genome shotgun sequence.
ACCESSION   DAOSIM010000261 DAOSIM010000000
VERSION     DAOSIM010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31971_length_2750_cov_7.968089"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..643
                     /gene="rpsD"
                     /locus_tag="PLR47_09580"
     CDS             14..643
                     /gene="rpsD"
                     /locus_tag="PLR47_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOG13240.1"
                     /translation="MARYTDSSCRLCRREGLKLFLKGDRCYSEKCSFERRGYAPGDHG
                     QSHKKQQSDYGTQLREKQKLKRMYGLLEKQFHGYFEKADRQKGITGTNLLVLLERRLD
                     NMIFRLGFANSRIEARQLISHGHFLVNGKAVNIPSYLLKAGDEVTVKEGSRKINRILE
                     AMETVARRGVPNWLGLDKDNFKATIKMLPVREDLTMPVQEQLVVELYSK"
     gene            688..>1143
                     /locus_tag="PLR47_09585"
     CDS             688..>1143
                     /locus_tag="PLR47_09585"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416241.1"
                     /note="catalyzes the transcription of DNA into RNA using
                     the four ribonucleoside triphosphates as substrates.
                     Dimerization of the alpha subunit is the first step in the
                     sequential assembly of subunits to form the holoenzyme;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOG13241.1"
                     /translation="MQRNWSSLIRPKRVDVDEATHTRFYGEFTIQPLERGFGITLGNA
                     LRRVLLSSIQGAAITSVKIDGVLHEFSTIPGVKEDVTDVILNLKGVRFKMGSADTKVV
                     KFNVTSHGVITAGDIITDGAIEVLNPDHYIATLSGGGSLKAEMVVKMGKG"
     assembly_gap    1144..1153
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1154..1625
                     /locus_tag="PLR47_09590"
     CDS             <1154..1625
                     /locus_tag="PLR47_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HOG13242.1"
                     /translation="TINIDAIFSPIKKVNYVVSHARVGQITDYDKLVLEVWTDGSLNP
                     GDAIAYSAKILKQQLDVFINFEEVEEDAAPEQESPGGNVNEILMRSVEDLELSVRSAN
                     CLKNAGINTIGELVQKTEAEMLKTKNFGRKSLSEIKDILNEYGLAFGMKPDENA"
     gene            1704..2108
                     /gene="rplQ"
                     /locus_tag="PLR47_09595"
     CDS             1704..2108
                     /gene="rplQ"
                     /locus_tag="PLR47_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013573706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOG13243.1"
                     /translation="MYHGKAGRKLGRSSSHREAMFRNMVTSIIRHESIRTTDATAKEV
                     RKLADRMITLGKRGDLHARRQALSVVRDKEMVAKLFGELAERFRNRAGGYTRIVKVGY
                     RFGDNAPVSILEYIPDENKKEKAKPKKAKKAE"
     gene            2221..2481
                     /locus_tag="PLR47_09600"
     CDS             2221..2481
                     /locus_tag="PLR47_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HOG13244.1"
                     /translation="MAEKKIGEVLKFFAKPSVAAVHLSEGELQVGDSIKFSGHTTEFT
                     EVVQSMEVDNKPVQKAVAGDSIGLKVSDRVRPGDEVFKILPD"
     gene            2572..>2750
                     /locus_tag="PLR47_09605"
     CDS             2572..>2750
                     /locus_tag="PLR47_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG13245.1"
                     /translation="MAKILVVDDEKDIADLVSYNLEREGFAVVKAFDGETALAKVRSE
                     KPDLLILDLMLPGVGG"
BASE COUNT          716 a          634 c          805 g          585 t
ORIGIN      
        1 aagggaggct tttttggcaa gatatacgga ttcctcatgt cgtttgtgcc gtcgtgaagg
       61 cttgaagctt tttttgaagg gcgatcggtg ttattcggag aaatgttcct ttgaacgaag
      121 aggctacgct ccgggagatc atggccagtc ccacaagaaa cagcagtcgg actacggcac
      181 ccagttgcgc gaaaagcaga agctcaaaag aatgtacggg cttctggaaa agcagttcca
      241 cggctacttt gaaaaggcgg accgccaaaa gggcattacg ggcacgaatt tgctggtgct
      301 tctggagcgc aggctggaca acatgatttt ccgcctgggg tttgccaatt cccgaatcga
      361 agcgcggcag ctcatcagcc acggtcattt tctggtgaac gggaaagcgg tgaacatccc
      421 gtcctatttg cttaaggcag gcgacgaggt aaccgtcaag gaaggctccc ggaagatcaa
      481 ccggattctg gaggccatgg aaaccgttgc ccggcgcggt gtgccgaatt ggctgggatt
      541 ggacaaagac aattttaaag cgaccatcaa gatgctgccg gtccgcgagg atctgacgat
      601 gccggtgcag gaacagcttg tggtagagct ttattcaaag taaaccgcca acagattgaa
      661 taaatcaact taagggtggg tttgattatg cagagaaact ggtcgagttt aattcgtccc
      721 aaacgtgtag acgttgatga agccacgcat acgcggttct atggagaatt taccattcaa
      781 cctctggaaa gaggatttgg aatcacactg ggcaacgcgt tgagaagggt tctgttgtcg
      841 tctattcagg gagcggccat cacatccgtc aagatcgacg gtgttttgca cgagttttcc
      901 acgattcccg gcgtcaagga agacgtcacg gacgtgattt tgaacctgaa gggcgtccgc
      961 ttcaagatgg gttcggcgga cacgaaagtt gtaaaattca acgtgaccag ccacggcgtc
     1021 attaccgccg gcgacatcat tacggacgga gcgatcgagg tcctgaatcc cgatcactat
     1081 atcgccacgc tgtccggcgg cggttcgctg aaggcggaaa tggtcgtgaa aatgggcaag
     1141 gggnnnnnnn nnncaccatc aatatcgacg ccattttttc gccgatcaag aaagtcaact
     1201 acgtggtatc tcacgcccgc gtcggacaga tcacggatta cgacaagctg gtgctggaag
     1261 tctggacgga tggaagcctg aacccgggcg atgccatcgc gtattcggcc aagattctga
     1321 agcagcagct ggatgtgttc atcaattttg aggaagtcga ggaagatgcc gctccggaac
     1381 aggagtctcc cggcggcaac gtcaatgaaa tcctcatgcg gtcggtggaa gacctggaac
     1441 tctccgtgcg ttcggccaac tgcctgaaaa acgcggggat caacacgatc ggggagctgg
     1501 ttcagaagac cgaggcggaa atgctcaaaa ccaagaattt cggacgcaag tccctgagtg
     1561 aaatcaagga tattctcaac gaatacggcc ttgccttcgg catgaagccg gatgaaaatg
     1621 cttaaaaagg aaaccgaccg gaacgcggga cggcgtttga tgatggattc atcaggggac
     1681 ggtcaggaaa ggataaagta accatgtatc acggtaaggc gggcagaaaa ctgggacgat
     1741 cgtccagcca tcgggaagcc atgtttcgca atatggtgac atcgatcatc aggcatgaaa
     1801 gcatccggac aaccgatgcg acggccaagg aagtgcgcaa gctggcggat cgtatgatta
     1861 cgctcggaaa aagaggcgat ctgcacgcgc gccggcaggc actgtcggtg gttcgggata
     1921 aagagatggt cgccaagctt ttcggcgagc tggccgagcg cttccgcaat cgggcgggcg
     1981 gctacacccg gatcgtcaaa gtcggctacc gctttggcga caacgcgccg gtttccattc
     2041 tggaatatat tccggatgag aacaagaaag aaaaggcaaa gccgaagaag gcaaaaaagg
     2101 ctgaatagcc aataaaactg aatcataaaa ggcagggcgc gcgccctgcc ttttttttgt
     2161 ggttgttttt tcaaatcgat caaggtattt atgggcaact actgcaaagg aggaatcgag
     2221 atggcagaaa agaaaatcgg cgaagtgctc aagttttttg cgaagccttc ggtggcggca
     2281 gtccatcttt cggagggcga attgcaggtc ggcgacagca taaaattttc cggccatacg
     2341 acggaattta ccgaagtagt ccagtccatg gaagtggaca acaagccggt gcagaaagcc
     2401 gtggcgggcg attccatcgg cctgaaagtc tcggaccgtg ttcgtcccgg agatgaagtt
     2461 tttaaaattc ttcccgacta acccccggta agcgtttaat cgaagccggc gtttattttt
     2521 aatgcaggcg atgcggattg ctgtccattc atgccgtgac agtctttgca aatggccaaa
     2581 attctcgttg ttgacgatga aaaagacatc gcggatctgg tgtcctataa tctggagcga
     2641 gagggattcg ccgtggtaaa agcctttgac ggcgaaacgg cgcttgccaa agtcaggtcc
     2701 gaaaaaccgg atttgctgat tctggatttg atgctgcccg gggtcggcgg
//
LOCUS       DAOSIM010000262         2739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_32139_length_2739_cov_7.388972, whole genome shotgun sequence.
ACCESSION   DAOSIM010000262 DAOSIM010000000
VERSION     DAOSIM010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32139_length_2739_cov_7.388972"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="PLR47_09610"
     CDS             complement(<1..1452)
                     /locus_tag="PLR47_09610"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOG13246.1"
                     /translation="MSEKTPTETGRPEEKYYSGCMYADQDLWRFHAAKAYLSRVSIAD
                     EDVQALKDLAGKGAVVYAIKQRSKLNSLILAEVTARKGLPMPVYSHGVNMSFWQPLSK
                     MMKFLWSSFLRRFRKNEIARQGKLEFLAGKVAARESVIIHLGESEFIESRASEDAIAG
                     LMNLQAGLPFPIYIVPVLVAYGRRREKEDESLFNILFGQTEHTGTMRRLITFIRYSKQ
                     AFILPAEPVHLPEYLSANSLLTKNELVMDLRGELIDRIEEEKTSVVGPALKSREAMMG
                     MVMHDPKLNGFLDEYARNNKKDRAGLARDVRRYLNEIASDYRETFVELWDKVLTWLWN
                     TVYDGVSIDSEGLANVRNISKKMPFVIISCHRSHIDYLLLSYVFYKNNIQLPFIAAGN
                     NLSFFPLGYIFRRSGAFFLRRSFRGNEVYGEVFSKYMAMLLQEGLPLEFFIEGGRSRT
                     GKMVMPKYGLLSMIIQAYQDKYCENLAAVPVYIG"
     gene            complement(1500..>2739)
                     /gene="ptsP"
                     /locus_tag="PLR47_09615"
     CDS             complement(1500..>2739)
                     /gene="ptsP"
                     /locus_tag="PLR47_09615"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOG13247.1"
                     /translation="MKMDKIVGFATDSGGRTSHTAIVARSMELPAVVGLDNVTRFVRT
                     GDDIIVDGTSGLVVVNPYPDMLKRYEEKKRHYDAAKDEYLKYARKPAVTVDGYSVKIG
                     SNIEFVEEIPSAVSHGAEHVGLYRTEFLYIYRDELPTEEDHFNNYRQVVEEKNFSWAT
                     IRTFDLGGDKFTKYQKHARELNPQMGLRAIRFCLKEIEIFKTQLRAIWRVSALGRVKI
                     LFPMISGMEEIREAKRLLEETRRELLAQGAPIADRMEIGAMIEVPAAAVIADQLATEV
                     DYFSIGTNDLIQYALAIDRSNERVTYLYEPLHPAVLRLIKRIVDSAHKAGIRVAMCGE
                     MAGDPLCCPILLGMELDELSMNHLAIPRIKRILQQSSLEESRKLLEKALSYNNAAEVR
                     ACVQDYMTRRFPDEIQKREL"
BASE COUNT          563 a          801 c          761 g          614 t
ORIGIN      
        1 gccgatgtac acgggcaccg cggccagatt ctcgcaatac ttgtcctgat aggcctgaat
       61 aatcatggac aaaagcccgt atttgggcat caccattttc ccggtgcggc ttcggccgcc
      121 ctcgataaag aactcgagcg gaagaccttc ctgaagcagc atggccatat atttggaaaa
      181 gacctctcca taaacctcgt tgccgcgaaa gctcctccgc agaaaaaacg cgccggaccg
      241 tcggaaaata taccccagcg ggaagaaaga aaggttgttt cccgcggcga taaacggaag
      301 ctgaatgttg tttttgtaaa aaacatagga caacagcaga taatcgatgt ggctgcggtg
      361 gcaggaaata atgacaaacg gcattttctt ggaaatattg cgcacgttgg ccagcccttc
      421 ggagtcgatg gacacgccgt catacaccgt gttccacagc caggtcagaa ccttgtccca
      481 caattccacg aacgtttccc ggtaatccga cgcgatttcg ttcagatagc ggcggacgtc
      541 ccgggccagg ccagcccggt cttttttgtt gttccgggcg tattcatcca ggaacccgtt
      601 cagcttcgga tcgtgcatga ccatgcccat catggcttcg cgcgatttca gcgccggtcc
      661 gaccacggaa gtcttttcct cttcgatccg gtcaatcagc tctccgcgca aatccattac
      721 gagttcattt ttggtcaaaa gactgttcgc ggacagatac tccggcagat gaaccggttc
      781 ggcgggaagg ataaaggcct gcttggaata gcgtatgaac gtgatgagac ggcgcatcgt
      841 tcccgtgtgc tccgtctggc cgaaaaggat gttgaaaagg ctttcatcct ccttctcccg
      901 tcgccggccg tacgccacga gcaccgggac gatgtagatg ggaaacggga ggccggcctg
      961 caggttcatc aggccggcaa tggcatcctc ggaggcgcgg ctttcgataa actccgactc
     1021 tcccagatga atgatgacgc tttcccgggc ggcgactttt ccggcgagga attcgagctt
     1081 cccctggcgg gcgatctcgt ttttccggaa gcggcgcagg aaagaagacc agaggaactt
     1141 catcatcttg gagagcggct gccaaaaaga catgttgacg ccgtggctgt ataccggcat
     1201 gggcaggcct ttgcgcgcgg tcacctcggc caggatcagg ctgttgagtt tgcttctttg
     1261 cttgatggcg taaaccacgg cccccttgcc ggcaagatcc ttcagcgcct ggacatcctc
     1321 gtcggcgatg gaaacccgcg acagataggc cttggcggca tgaaagcgcc agagatcctg
     1381 atcggcatac atgcagccgg agtaatattt ctcttccggc cgtccggttt ccgtgggcgt
     1441 tttttcgctc atactctcca gtctcttcgt ttgccgggcc cgggattcgc gtccggcgcc
     1501 tacagttccc tcttctgaat ctcatcggga aaacgccggg tcatgtagtc ctggacgcag
     1561 gcccgaactt cggcggcgtt gttgtaggac agggccttct ccagaagctt tcgggattcc
     1621 tccaggctgg actgctgaag gattctcttg atgcggggaa tggccagatg gttcatgctc
     1681 agttcgtcca gctccatccc cagaaggatc ggacagcaaa gaggatcgcc ggccatctct
     1741 ccgcacatgg ccacgcgaat gccggccttg tgcgccgagt ccacgatgcg tttgatcagc
     1801 ctcagaacgg ccggatgcag gggctcgtag aggtaggtga ccctttcatt ggagcggtca
     1861 atcgccagcg cgtactgaat caggtcattg gtgccgatgc tgaaataatc gacttccgtc
     1921 gccagctggt cggcaatcac ggcggccgcc ggcacttcaa tcatcgcccc gatttccatc
     1981 cggtcggcga tcggcgcccc ctgggccagc agctcccggc gagtctcttc cagcagccgc
     2041 ttggcttccc ggatttcctc catgccggaa atcatcggga ataaaatttt caccctgccc
     2101 agcgcgctga cgcgccagat cgcgcgaagc tgtgttttga aaatctcgat ttccttcagg
     2161 cagaagcgga tggcccgcag gcccatctgc ggattcagct ccctggcatg cttctgatat
     2221 ttggtgaact tgtcgccgcc cagatcaaac gtgcggatgg tcgcccagga aaagtttttt
     2281 tcctcgacca cctgccggta gttattgaag tgatcttctt ccgtcggcag ttcatcgcgg
     2341 tagatataga gaaattccgt ccggtacagc ccgacgtgct ccgccccgtg cgacaccgcc
     2401 gaagggatct cttcgacaaa ttcaatattg gaaccgattt tcacgctgta gccatccacc
     2461 gtcacggccg gcttgcgggc gtatttcaga tattcatcct tggcggcatc gtagtggcgc
     2521 ttcttttcct catacctttt gagcatgtcg ggatagggat tgaccaccac gagccccgac
     2581 gtgccgtcca cgataatgtc gtcgccggtg cggacaaagc gggtgacatt atccagcccg
     2641 acgacggcgg gcaattccat ggagcgggcc acgatcgccg tgtgggacgt ccggccgccg
     2701 ctgtcggtgg caaaaccgac gattttatcc atcttcatc
//
LOCUS       DAOSIM010000263         2728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_32332_length_2728_cov_6.412271, whole genome shotgun sequence.
ACCESSION   DAOSIM010000263 DAOSIM010000000
VERSION     DAOSIM010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32332_length_2728_cov_6.412271"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2360
                     /locus_tag="PLR47_09620"
     CDS             <1..2360
                     /locus_tag="PLR47_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13248.1"
                     /translation="EEINMMLSAAARQDQATGDRFVTEYDALTEAFRKEANTARRAFS
                     EGKKEEVERSKEAMRWLVKRRKSLQNVRDYFNLTDAEMMSATNRRNPALMDDAEFKQF
                     LRNVELKAVELAETRQEKMALLKLIEDKRLKKVDNYRRALELPTFNDMTAQQLRDFAK
                     LLEPFQDDDIFLTQRELETVDRTDLKGIKTWREARERLAKEAGVPVEELNKVHVEALD
                     SFRWDTSLRERDPFFGILVEQMTQSYLGAQLRAHEIENRVYELARKSEKSRDRGIIDR
                     MIPQDDLIMAFLEAPMDRKAAIVEQMTPEQIDFANYMQQYFAGALEYLIATKSIERGR
                     ENYFVHIRKTFLENLKDGGLKKAVSEIFKTYQQDQMVFNILDDDTGKILPLEKFFQFS
                     LQRTDTMDPTRNVTKAFLTYVNTFEKKKMYDAIIPKLDIYAQALTPTRYTPRGLEIDR
                     SLKTFVNKWINNKKGRRISFDSTLRQGGPLDVGIRALRAFTTILDLGLSPVVQTAAFL
                     GEQVTNAAMLGIKAMAIGTARMRTAQGKAILKKYESWVGRSLWEEFTVPGKEITERLS
                     HALFAGFHLSSVFANKQFLLASLTEQEWATGEISTERLAEMRLDMGRFRVVPGTGSLV
                     GSTSVGDAVMQYKKWAAPFARTLAMDAQKFLGDLKSKPIGEALTSREARELYRLAYLS
                     MAVFVVGAMAGADDDDKSVMGKAKARMYREAMTLMQGVDPTLWLASPRLLTWLYQTAS
                     ALKSVALMETYKSGDKEGELKGPDEMKRQFVPGVVRSVSKEENKR"
BASE COUNT          698 a          639 c          772 g          619 t
ORIGIN      
        1 ccgaagaaat caatatgatg ctatcggccg cggccaggca ggaccaggcg acgggggatc
       61 ggtttgttac agaatacgac gctttgaccg aggctttccg gaaagaagcg aacacggcac
      121 ggagggcctt ttcagaaggt aagaaagaag aggttgagcg ctcgaaagag gctatgcgct
      181 ggttggtgaa gcgccggaaa tctctgcaaa acgtccggga ttacttcaat ttgacagacg
      241 ctgaaatgat gagtgccaca aaccgccgga atccggcctt gatggatgac gcggagttta
      301 agcagtttct ccgaaacgtg gaattgaaag ccgttgagct tgcggaaacc cgtcaagaga
      361 agatggcatt acttaagctc atcgaagata agcgcttgaa aaaggtggac aactaccggc
      421 gagcgctgga actgcctacc tttaacgaca tgaccgccca gcagttacgg gactttgcga
      481 aactgcttga gccgttccag gatgacgata ttttcttaac gcaaagggaa ttggaaaccg
      541 ttgaccggac ggaccttaaa gggatcaaaa catggcgcga ggcgcgagag cgattggcga
      601 aagaggcggg tgtgccggtc gaggaactga ataaggtcca cgtcgaggcg cttgactcat
      661 tccggtggga cacgtccttg agagagcgag accccttctt cggtatcctt gttgaacaga
      721 tgacgcaatc atatttgggg gcgcaactgc gggcgcacga aattgaaaac agggtttatg
      781 agctggcccg gaagtctgag aagtccagag acaggggcat tattgacagg atgattcctc
      841 aagacgatct gatcatggcc ttccttgagg ccccgatgga ccgcaaggcg gccatcgtcg
      901 agcagatgac gccggagcaa atcgacttcg caaactacat gcagcagtat ttcgccgggg
      961 cgctggaata tctgattgcc acaaaaagca ttgagcgtgg ccgggaaaac tattttgtgc
     1021 atatccggaa aactttcctt gagaatttga aagacggtgg cttgaaaaaa gctgtttcgg
     1081 agattttcaa aacatatcag caggatcaaa tggtttttaa tatcctcgat gacgataccg
     1141 gaaagatcct ccctctggaa aagttctttc agttcagttt acagcgaacc gacacgatgg
     1201 acccgacgcg caacgtcacg aaggcgtttt tgacctatgt caacactttt gaaaaaaaga
     1261 agatgtacga cgccattatc ccgaagctgg acatttacgc gcaagcgctg acgccgacgc
     1321 gatacacgcc gagagggctg gaaattgaca ggtcattgaa aaccttcgtc aataagtgga
     1381 tcaataacaa aaagggcagg agaatatcct ttgattcgac gctacggcag ggcgggcccc
     1441 tggatgtggg gattcgggcg ctgcgggcat tcacaacgat cctggacctg gggctttctc
     1501 cggttgtcca gactgcggcg tttcttggcg aacaggtgac aaatgcggcc atgctgggaa
     1561 ttaaggccat ggctatcgga acggcacgga tgcggacagc acaaggcaag gcaatcctga
     1621 aaaaatatga atcatgggtt ggccggtcgc tttgggaaga atttacggtt ccgggcaagg
     1681 aaattactga acgtctgtca catgcccttt ttgccgggtt tcatctgtcg agcgtgtttg
     1741 ccaataagca gtttctttta gcctcactga ccgaacagga atgggcgacg ggggaaatca
     1801 gcacggagag actggccgaa atgcggctgg atatggggcg cttccgtgtt gttccgggta
     1861 ctggttcgct ggttgggtca acgtccgttg gtgatgctgt catgcagtat aaaaaatggg
     1921 cggctccttt tgcgcggacg ctggccatgg atgcccagaa gtttctcggg gatctgaaat
     1981 ccaaacctat cggggaggct ttaacgagca gggaggcgcg ggagctttac cggctggctt
     2041 acctatcgat ggctgttttt gtggtcgggg cgatggccgg agctgatgac gatgataaat
     2101 cagtaatggg aaaagccaaa gcccggatgt atcgggaagc aatgaccttg atgcagggcg
     2161 ttgacccgac gttatggctg gcgtcgccgc gcttgctgac gtggctctac caaacagcct
     2221 ccgctttaaa gagtgtggca ctgatggaaa cctataaatc cggagacaaa gaaggcgaat
     2281 tgaaagggcc ggatgaaatg aagcgtcagt ttgtgccggg cgtcgttcgg agcgtatcga
     2341 aagaagagaa caagcgttga catcctcccc ggcctgaagg acggggcttt acggcccgtc
     2401 tgataaaccc cttttatatc ctcaatctta cgccggtagt aggcagacct ctgcgccttg
     2461 tattcggctt catacgcttt tttttggctc atgcaccctc ctacaagtcg tcccgtccca
     2521 ggggtttatc cagatgcacc acgtccgcat cccaggcata aaataaaggc actgcttctg
     2581 ctcgtcgatc gactggcaga ggcaacgccc ttttgactgt ttccatgttt tttctttttt
     2641 atcacacatt atttaataac atccttccac gacaacccca cgttattaca caacttatca
     2701 attaaacgca gttgttcttg aaattttt
//
LOCUS       DAOSIM010000264         2719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_32469_length_2719_cov_6.153904, whole genome shotgun sequence.
ACCESSION   DAOSIM010000264 DAOSIM010000000
VERSION     DAOSIM010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32469_length_2719_cov_6.153904"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..691
                     /locus_tag="PLR47_09625"
     CDS             197..691
                     /locus_tag="PLR47_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOG13249.1"
                     /translation="MKAQDTELLQEFTPEQVAQLDRIIEKHKGQAGGLIPVLEEAQVC
                     LQYLPLPVQRRIAAGLNLPLSRVYGVVTFYSFFTMTPRGKHTIRVCLGTACYVRGGKE
                     LTQSLEKQFGIKDGETTADRMFTLESVRCLGACGLGPVIVVNEDVHGRVKPAKIKELL
                     SQYN"
     gene            721..1104
                     /locus_tag="PLR47_09630"
     CDS             721..1104
                     /locus_tag="PLR47_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOG13250.1"
                     /translation="MARLTIDDLKKIKEKVHKEMSLRDGDRRVKVTVHMGTCGIASGA
                     KEVMDSLLEEIESAKVNDVIVTTSGCMGLCSREPLVTVEVVNQEPIKYEYVNPNKMRQ
                     IFKRHILAGEVQTPFVLAKGAEIVK"
     gene            1169..>2719
                     /locus_tag="PLR47_09635"
     CDS             1169..>2719
                     /locus_tag="PLR47_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOG13251.1"
                     /translation="MKIFRSHLLLCGGTGCHASGSPGVKSALLEEIVKRKLADEVKVV
                     ETGCNGFCALGPIMVVYPEGVIYMNLKPEDMPELVEEHLVKGRVVEKLLYREPGTDKT
                     IPTMQDIPFFAHQELRALRNRGLIDPEKIEEYIARDGYQGMAKALTEMTPEQIVQEIL
                     DSGLRGRGGAGFPTGLKWKFAAQSKGDIKYVLCNADEGDPGAFMDRSILEADPHSILE
                     GMVIAAKAIGAHHGYIYCRAEYPLAIHRLNTAIEQAKEAGLLGNDILGTGFNFDLEIY
                     QGAGAFVCGEETALMTSIEGKRGMPRPRPPFPAVAGLWQKPSVLNNVETLANVGQIIQ
                     RGAKWHSSIGTEKSKGTKVFALTGDVNNVGLVEVPMGTTLGTIVYDIGGGIPNDKKFK
                     AAQLGGPSGGCIPIEHLNAPVDYEKVAELGAIMGSGGLIVMNEDKCAVDMARFFMDFC
                     VDESCGKCTPCREGTKRMLDILTDITQGKGKPGDIELLEDMAEVIKNAALCGLGQTAP
                     NPVLSTIRY"
BASE COUNT          725 a          704 c          735 g          555 t
ORIGIN      
        1 atgttgattt tttttacaaa cacaggcata atgaccgccg gtttgaaatg gatacgcgcg
       61 atattcgcaa tgaaatcgag gatgtgccca taattcatcc cgaaatcatt aaatatataa
      121 gaggcgtctt gaccgagggg atcgctcaaa ttcaatccga agcctgatgc gcctaccgaa
      181 ccgaggagga atttaaatga aagcccaaga cacagaactt ttacaggagt tcacacccga
      241 acaggtggcc cagctcgacc gaatcattga aaaacacaag ggacaggcag ggggactgat
      301 ccctgttctg gaagaagcgc aggtctgtct tcaatatctg cccctgccgg tgcaaagaag
      361 aatcgccgcc ggtttgaatc ttccattaag ccgcgtgtac ggcgtggtca ctttttattc
      421 atttttcacc atgacaccac gcggaaaaca tacaatccgc gtttgtctgg gcaccgcctg
      481 ctacgtgcgc ggcggcaagg aattgacgca atccctggaa aaacagttcg gcatcaaaga
      541 cggagagaca actgcagacc gcatgttcac gctcgaatcc gtccgctgcc tcggcgcttg
      601 cggtctgggt ccggtcatcg tcgtgaatga agacgtgcac ggacgcgtca aaccggccaa
      661 gatcaaagaa cttttaagtc agtataatta acctttcagc atataacgga ggaaacaccc
      721 atggcaagat taacaatcga tgatttaaaa aaaatcaaag aaaaggtgca taaggagatg
      781 tctttgcgcg atggagaccg tcgcgtcaaa gtcactgttc atatgggaac ctgcggcatc
      841 gcttccggcg ccaaagaagt aatggattcc ctgctggaag aaatcgaatc tgccaaagtc
      901 aacgacgtaa tcgtgacgac atcggggtgt atggggcttt gcagccgcga gccgctggtt
      961 actgtcgagg ttgtcaatca ggaaccgatt aagtatgaat atgtcaatcc caataaaatg
     1021 agacagattt ttaaaagaca cattctggcc ggagaggtgc aaacgccatt tgtcctggcc
     1081 aagggtgcgg aaatcgtaaa ataaaatatg accggtcgcg cgtcagggac gctgatgcgt
     1141 atggatcgtt aaggaggata tggtatcaat gaaaatattt cgttctcact tgctgttgtg
     1201 cggcggcacc ggctgtcacg catccggcag cccgggtgta aaaagcgctc tgctggaaga
     1261 aatcgtcaaa agaaagctgg cggatgaagt gaaagtcgtg gaaacgggat gcaacgggtt
     1321 ttgcgcgctc ggtcccatca tggtggtcta tccggaaggt gtgatttaca tgaatctcaa
     1381 accggaggat atgcccgaac tggtggaaga gcacctcgtc aagggacgcg tggtcgaaaa
     1441 actcctctat cgcgaaccgg gcacggacaa gaccatcccc accatgcagg acatcccttt
     1501 cttcgcccat caggagctga gggcgctgag aaaccgcggt ttgattgatc cggaaaaaat
     1561 tgaagaatat atcgcccgcg acgggtatca gggaatggcc aaggcgctca cggaaatgac
     1621 gccagaacaa attgtccagg aaatcctcga ttcaggactg cgcggccgcg gcggagcagg
     1681 attccccacc ggcctgaaat ggaaattcgc cgctcaatcc aaaggcgaca ttaaatacgt
     1741 actgtgtaac gccgacgaag gcgatccggg agcgttcatg gatcgctcaa ttctggaggc
     1801 cgacccccac tccattttgg aaggcatggt catcgccgcc aaagccatcg gcgctcatca
     1861 tggctatatc tactgccgtg ccgagtaccc gctggccatt caccgcctga ataccgctat
     1921 tgagcaggcc aaggaagccg gccttttggg taatgatatt ttgggaacag ggttcaattt
     1981 cgatctggaa atctaccagg gtgcgggagc tttcgtttgc ggtgaggaaa cagcgctgat
     2041 gacctcgatt gaaggcaaac gcggcatgcc ccggccccgt cctcccttcc cggccgtggc
     2101 cggcctgtgg cagaaaccca gcgtcctcaa taacgtggaa acactcgcca acgtaggaca
     2161 gatcatccag cgcggcgcca aatggcactc atccattggc acggaaaaga gcaaaggcac
     2221 aaaggttttc gccctcaccg gcgacgtcaa caacgtcggg ctggtcgaag ttcccatggg
     2281 aaccactctg ggtacgatcg tctatgatat cggcggcggc attcccaatg acaagaaatt
     2341 caaagcagcg caactgggcg gcccctccgg cggctgtatt ccgattgagc acttaaacgc
     2401 tccggtcgat tacgaaaaag tagcggaact gggcgccatc atgggctccg gcggccttat
     2461 cgtcatgaac gaggacaaat gcgccgtcga tatggcacgg tttttcatgg acttctgcgt
     2521 ggacgaatcc tgcggtaaat gcaccccctg ccgcgaagga acaaagcgca tgctcgatat
     2581 cctgacagac atcacccaag gtaaaggcaa gccgggagat atcgaacttc tggaagatat
     2641 ggccgaggtc attaaaaacg ccgctctgtg cggcctggga cagacggcgc ccaaccccgt
     2701 tttgagcacc atccgctac
//
LOCUS       DAOSIM010000265         2705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_32679_length_2705_cov_8.048679, whole genome shotgun sequence.
ACCESSION   DAOSIM010000265 DAOSIM010000000
VERSION     DAOSIM010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32679_length_2705_cov_8.048679"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PLR47_09640"
     CDS             <1..344
                     /locus_tag="PLR47_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13252.1"
                     /translation="AFLHPFTIHHPPFTIFVRGYSSAQSVTEPSPTGNDAYLKYFALS
                     ITYEVWPRPETCNSVWHVLLKLLHLSRKERSRAFPAFHCPIIAKFIEAGLKTRPTSFF
                     DELFPMAYRLF"
     gene            complement(383..1297)
                     /locus_tag="PLR47_09645"
     CDS             complement(383..1297)
                     /locus_tag="PLR47_09645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG13253.1"
                     /translation="MEISFIIVNWNTKDLLRGCLDSIAKTAGSLLHEIIVVDNASSDG
                     SADMLTREYPSVRLIANSENRGFGAANNQGFAVMKGKYALLINTDAVLTEGAAGKLWS
                     FAEANPRAAIVCGQLLNADGSKQNSVAAFPTLLTLCLNTSLLEYLFPGRYPSKRYDHR
                     EPLEVESAIGACMMVRGQALKEVSFFDERYFFFFEETDLAFAMKRAGWSVCQVPDALI
                     YHFQGKSIGHNAKSRIEFYRSRYQFLRKWHGSAYYRAAAAVIFLRLLADALLNAAGVV
                     FTLGLSKNLRRKLAVYTQLIRWHFEGKR"
     gene            complement(1307..2158)
                     /locus_tag="PLR47_09650"
     CDS             complement(1307..2158)
                     /locus_tag="PLR47_09650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG13254.1"
                     /translation="MEKIPLSVAIITKNEEENLRDCLQSVAFAAQIVVVDSASSDATP
                     AIAAEFGCDFYTESWHGFGPQKQIAIDRCSRPWILVLDADERIPPETAEVIRKITAGE
                     LAAAAGAVKTSGSPADASASVNGFSFPRKNFFAGRWIRHAGWWPDRVVRLFRRQAGRM
                     TQAAVHEAVEVDGRIIELDVPIEHYTESSLGRILQKIDKYSTLGAQEAFAAGKRSSPA
                     AALFRAFLTFVQGYILRLGFMDGRQGLTLAVTDAVNKFFKYAKLSELEEAGRGETRRS
                     SSSRPNA"
     gene            2407..>2705
                     /locus_tag="PLR47_09655"
     CDS             2407..>2705
                     /locus_tag="PLR47_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG13255.1"
                     /translation="MSKIAVVIPKYGLAGGAEQFASELTGRLRDKTKDAFHVLANRWA
                     SDSGVVRFEKIPIVSFPKWLTTISFAWFAQRQIRRGNFDLVHSHERIFAADLFTL"
BASE COUNT          586 a          761 c          740 g          618 t
ORIGIN      
        1 ctgcatttct ccacccattc actattcacc atccaccatt cactatattt gtccgcggat
       61 acagctccgc ccagtcggtt acagaaccat cgccgacggg aaacgatgca tacctaaaat
      121 attttgcact gtcaataact tacgaggttt ggccacggcc agaaacctgc aatagcgtct
      181 ggcacgtttt attaaagttg ctgcatctgt cgcgcaagga acgctcgcga gcgttccccg
      241 catttcactg cccaatcatt gctaaattca ttgaagcggg tctgaagacc cgccctacat
      301 cttttttcga tgagctgttt cctatggcct atcgcctttt ttaacttcca ccattcacca
      361 ttcactatcc accatttttc actcatcgct tcccctcgaa gtgccagcgg atgagctggg
      421 tgtaaacggc cagtttccgc cggaggtttt tagacaagcc caatgtgaac acgacgcccg
      481 cggcgttgag cagggcatcc gcgagcagcc tgagaaaaat aaccgcggcg gcggcccggt
      541 aataagcgct tccgtgccac ttgcgcagga actggtagcg cgaccggtaa aattcaatcc
      601 gcgattttgc gttgtgtccg atgcttttgc cctgaaagtg atagatgagc gcatccggaa
      661 cctggcagac gctccacccg gcgcgcttca tcgcaaaggc caggtccgtt tcctcaaaaa
      721 agaaaaagta gcgctcgtcg aaaaaagata cttctttcaa cgcctggccg cgcaccatca
      781 tgcacgcgcc gatggcggat tcgacttcca gcggctctct gtggtcatag cgcttgctgg
      841 gatagcggcc gggaaaaaga tattcaagca atgatgtgtt caggcaaagc gtgagcagcg
      901 tgggaaatgc ggcgacggaa ttctgtttgc tgccgtcggc attgaggagc tggccgcaga
      961 caatggccgc acggggattg gcttcggcaa aactccaaag cttgcccgcc gcgccttcgg
     1021 taagaactgc gtccgtgttg atgagcaggg cgtatttgcc cttcatgacg gcaaagccct
     1081 gattgttggc ggcgccgaat ccgcggtttt ccgaattggc gatcagccgg acggaaggat
     1141 attcccgcgt cagcatgtcc gccgagccgt ccgaagaggc gttgtccacg acgatgattt
     1201 catgcaaaag ggaaccggcg gttttggcga tggaatccag gcagccgcgc agaaggtctt
     1261 tggtgttcca gttgacgatg ataaaagaaa tttccattgc tgaatttcac gcattcgggc
     1321 gggaggacga tgaccggcgc gtctcgccgc ggcccgcctc ttccagctcg ctcagtttgg
     1381 catacttgaa aaatttattg accgcatcgg tgacggccag cgtgaggccc tgccgcccat
     1441 ccatgaatcc gaggcgcaaa atgtacccct gcacgaaggt tagaaaagcg cgaaaaagag
     1501 cggcggcggg cgatgagcgc tttccggcgg caaaggcttc ctgggcgccc agcgtcgagt
     1561 acttgtcgat tttctgcaaa atcctgccaa ggctgctttc tgtgtaatgc tcgatgggga
     1621 cgtccagctc gatgatccgg ccgtccactt cgacggcttc atgtacggcg gcttgggtca
     1681 ttctgcctgc ctgcctgcgg aacaggcgga cgacgcggtc gggccaccag ccggcatgcc
     1741 ggatccagcg gcccgcgaaa aaatttttgc gcggaaaact gaagccgttg acacttgctg
     1801 atgcatccgc cggggaaccg gaagttttta ccgcacccgc ggccgcagca agttcacccg
     1861 ccgtaatctt tcgaatcact tccgccgttt ccggtggaat ccgctcgtcc gcgtccagca
     1921 caagaatcca gggccggctg caccgatcga tggcgatctg cttttgcggg ccgaaaccgt
     1981 gccaggattc ggtgtaaaag tcgcatccga attccgcggc aatcgcaggc gtcgcgtcgc
     2041 tgcttgcgga atcgaccaca acaatctggg cggcaaaggc aacgctttgc aggcaatcgc
     2101 gaaggttctc ttcttcgttt ttcgtgatga tcgccacgga aaggggaatt ttttccatcc
     2161 aatacctgaa tatttcgaag atttttgttt gcccgtctct ttaaagcaca attccggcgg
     2221 cgacgcaaat cctatattag gccatgattt ttttcttcaa ctgtgtttat atgaaaagcc
     2281 tccctctctg tatccctccc cctcgatggg ggagggaagg gtgggggtga ttattatccg
     2341 ttgtgcccga ctcgggtatg aaggttagtg ctggcgtcag aaaaaacagc caaaagaaat
     2401 atttttatga gcaaaatcgc ggtggtgatc cccaaatacg gactggcggg cggcgcggaa
     2461 cagttcgctt ccgagctgac cggcaggctt cgcgacaaaa ctaaggacgc ctttcatgtg
     2521 ctggccaacc gctgggcctc ggattccggc gtggtccggt ttgagaagat ccccatcgtt
     2581 tcgttcccca aatggctgac gacgatcagc ttcgcgtggt ttgcgcaacg ccaaatccgg
     2641 cgcggcaatt ttgacctggt gcacagccac gaacggattt ttgcggccga tttgttcacg
     2701 ctgca
//
LOCUS       DAOSIM010000266         2692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_32868_length_2692_cov_7.830489, whole genome shotgun sequence.
ACCESSION   DAOSIM010000266 DAOSIM010000000
VERSION     DAOSIM010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32868_length_2692_cov_7.830489"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PLR47_09660"
     CDS             complement(<1..762)
                     /locus_tag="PLR47_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HOG13256.1"
                     /translation="MDIETRKQNGILTIEFNRPDKKNSITAAMYQQMADAIQAGEDDR
                     AVRVLLFCGKPEIFSAGNDLEDFMNTKSDVADRPVAQFIRNLSLAAKPVIAAVSGMAI
                     GIGVTMLLHCDQIYAAQGAKFSMPFAKLGLCPEFASSRILPQIVGYQRAAELLFFGEP
                     FTAEQAFDMGLVNKVLPAAELMPYASAQAARLAALPPTSLRVTKQLLKARHLDAVTQQ
                     IAEENRHFSAMLKAPAAKEAFAAFYEHRKPDYSKIF"
     gene            complement(788..>2692)
                     /locus_tag="PLR47_09665"
     CDS             complement(788..>2692)
                     /locus_tag="PLR47_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOG13257.1"
                     /translation="SIIGFLITGILIGPFGLKLIDDIAGISLMADIGVAFLMFTIAVE
                     IRLSRFLKHLSEILLTGGLQLFCTFVVGLLAGLAMQLSVSQSVLIGFLLVHSSSALIL
                     KILKDRDDESSPQGRISVGVILFQDIMVVPMMLMIPFLAAGSGPDALLILWKLFKSLL
                     IIVVILVAGRYVIPFALERLVSMNMRDVLVIVSVVITMGIAWITESLGLSLAIGAFLA
                     GLALSDTDFTHQIISDVSPFRDVFLSVFFVSFGMILNLDFLKAHPGPILLASLGIILI
                     KFAVVASLVFLMKYPLRSAILCGVLLSQIGEFSFVLASQGYRSQIIAGDIYQTFIGAS
                     VLTFVITPLLASLAYGLLNRKNSSHSDSTGPLQGQRISLYNHVIICGMGLNGRNLARV
                     LKNTAISYAMIDLNFQKIKKAKNGGDTNALWGDASNVEILRRAGVEKARVMVIAISDR
                     FLTKNCLSNAKALNPKLHVIVRTKYLADIEELLALGADDVIPEEFETSIQIFSRVLKM
                     FHIPNSIVLAQGNIIRNKSYGILREVRYTQEAFDQIGQILAQGTIETCYIAPGNPTIG
                     KSIRDVNLKAETGAMIINIIRKNQTITNPPGDFVFEAEDQLILFGSHSAIDLGLKKLE
                     GNPADGSPAV"
BASE COUNT          596 a          707 c          846 g          543 t
ORIGIN      
        1 gaaaatcttc gaataatccg gtttgcgatg ctcgtagaaa gcggcgaagg cttccttggc
       61 cgccggcgct ttaagcatgg cgctgaaatg ccggttttct tcggcgattt gttgggtcac
      121 cgcgtcgagg tgccgcgctt tgagaagctg cttggtgacg cgcagggagg tgggcggcag
      181 ggcggcaagc ctggcggcct gagcagaggc gtaaggcatc agctccgctg ccggcagcac
      241 tttgttgacg agccccatgt cgaaagcctg ttcggccgta aagggttcgc cgaaaaagag
      301 cagctccgcg gcgcgctgat agccgacaat ctgcggcaga atacggctgg aggcaaattc
      361 cgggcacagc cccagtttgg caaacggcat ggagaatttg gcgccctgcg cggcgtaaat
      421 ctggtcgcag tgcagaagca tcgtgacgcc gatgccgatg gccattcccg aaaccgcggc
      481 gatcaccggt ttggccgcga ggctcaaatt gcggatgaac tgcgccacgg gacggtccgc
      541 gacatcgctc ttcgtattca tgaaatcttc cagatcgttg cccgcggaaa aaatttccgg
      601 tttgccgcaa aagagcaaaa cgcgcacggc ccggtcgtct tcgccggcct gaatcgcgtc
      661 cgccatctgc tggtacatgg cggccgtaat ggagtttttc ttgtccggcc ggttgaattc
      721 gatggtcagg attccgtttt gctttctggt ttcgatgtcc atttgttctc cttaagggtt
      781 taaaaaatta taccgccggg gatccgtcgg cggggtttcc ctccagcttc ttcagcccca
      841 ggtcgatggc gctgtggctg ccgaaaagaa tcaactgatc ttccgcctcg aaaacaaaat
      901 cgccgggcgg attcgtgatg gtctggtttt tgcggatgat gttgatgatc atggcgcccg
      961 tttccgcctt caggttgacg tcgcggatgc ttttgccgat cgtcgggttg ccgggggcga
     1021 tgtagcaggt ttcaatggtg ccctgcgcca gaatctggcc gatctggtcg aaggcctcct
     1081 gggtatagcg cacttcgcgc aaaatgccgt aggatttgtt ccggatgatg ttgccctgcg
     1141 ccagcacgat gctgttggga atgtgaaaca tcttgaggac gcggctgaag atctgaatgg
     1201 aggtctcgaa ttcctcgggg atgacatcgt cggcgcccag ggccagcagt tcctcgatgt
     1261 cggcaaggta tttcgtccgg acgatcacgt gcagcttcgg attgagggct ttggcgttgg
     1321 acaggcagtt tttcgtaaga aaacggtcgg agatggcgat gaccatgacg cgcgccttct
     1381 ccacgccggc gcgccgcaga atttccacgt tggacgcgtc gccccagagc gcgtttgtgt
     1441 ccccgccgtt tttcgccttt ttgattttct ggaaattcag atcgatcatg gcgtagctga
     1501 tggcggtatt tttcagcacc ctggccagat ttctgccgtt gagccccatc ccgcagataa
     1561 tcacgtggtt gtaaagggaa atgcgctgac cctgaagggg gccggtggaa tcggaatgcg
     1621 aagagttttt gcggttgagc agcccgtagg ccagcgaggc caaaaggggc gtgatgacaa
     1681 aagtcagaac cgacgcgccg atgaaggtct gataaatgtc cccggcaatg atctggcttc
     1741 ggtagccctg cgaggccaga acaaaggaaa attcgccgat ctgggacagc aggactccgc
     1801 agagtatcgc gcttcgcaaa ggatacttca tcaggaagac caggctcgca accacggcaa
     1861 acttgatgag aatgatcccc agagaggcca gcaggatggg tccgggatgg gccttgagaa
     1921 aatcaagatt gagaatcatg ccgaaggata cgaaaaacac ggacaggaag acgtcgcgga
     1981 aggggctgac atcgctgatg atctggtgcg tgaaatccgt gtcggaaaga gccaggccgg
     2041 caaggaacgc gccgatggcg agcgacagac ccagcgattc cgtgatccag gcgatcccca
     2101 tggtgatgac cacggacacg atgaccagca cgtcgcgcat gttcatgctg acgaggcgct
     2161 ccagcgcaaa cgggatcacg tacctgcccg cgaccagaat cacgacaatg atcaggaggg
     2221 acttgaaaag cttccagaga atcagaaggg cgtccggacc gctgccggcg gccagaaagg
     2281 ggatcatcag catcatcggc acgaccatga tgtcctggaa taaaatgacg ccgacggaga
     2341 ttcgcccctg cggcgacgat tcgtcgtccc gatccttgag gattttcaga atcagcgccg
     2401 aggagctgtg gaccagcaga aagccgatca ggacggactg gctgacgctg agctgcatgg
     2461 ccagtccggc gagcagtccg acgacgaagg tgcagaaaag ctgcagaccg cccgtgagga
     2521 gaatctcgga gaggtgcttc agaaagcggg acagccggat ctcgacggcg atggtgaaca
     2581 tcagaaatgc gacgccgatg tccgccatca gtgaaatgcc ggcgatgtcg tcgatcagtt
     2641 tcagaccgaa aggcccgatc agaatgccgg tgatgagaaa gccgatgatg ga
//
LOCUS       DAOSIM010000267         2681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_33073_length_2681_cov_7.955826, whole genome shotgun sequence.
ACCESSION   DAOSIM010000267 DAOSIM010000000
VERSION     DAOSIM010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33073_length_2681_cov_7.955826"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1536)
                     /locus_tag="PLR47_09670"
     CDS             complement(<1..1536)
                     /locus_tag="PLR47_09670"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOG13258.1"
                     /translation="MKKSYSEKVVSAETAIRSISRGKRVFIGSYCGEPQHLVSALLDH
                     TDHFSDVEVVRFLNLEGSLMGLVAEETQGRNYHVRSIYQGSGMIRGLTASRRFLTPMN
                     LYTVPSLFQKRHIPIHYALIQTAPPDEFGWLNLGISVDITLAAAHAADVVIVQVNPNM
                     PVVPGYGMIHVDEVDFIVEHEEDLLSVYPTPRIASTDRIAKLLSSLIEDGSTLHISPG
                     FSEELVLEALGEKQNLGIHSLMILDVMKKLAEKGVITNRKKGFNDGKMVASGAIGSEG
                     LYRYLNGNPAIEFRPCDYVSNPVMIARHHKMTAVNRVTSIDLKGQVAADGSAQNHFSD
                     VAGLVDFSRGAAMAPGGKSIVVVPSASEDGLSSNIVLEQAAGTVAIPAADVTYVVTEY
                     GAVNLFGKNVQERAMAMISVAHPDFRESLFEQARREGLIGQERKLYESQFGVYPAWLE
                     EVLTIAGQKVTLRPVKTVDDRLIQEHFYEMEEKDVAKRFFGRKHHFYWDEMKDMFIVD
                     YAKN"
     gene            complement(1547..>2681)
                     /locus_tag="PLR47_09675"
     CDS             complement(1547..>2681)
                     /locus_tag="PLR47_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13259.1"
                     /translation="GGWIQRNIPTAGLLGSIGGVGLMLLGFLPMIEIFNEAIVGLVAL
                     GLIFAALYSRLQFPGRIHGVVAALLVGTGIHFALGWSGCLPEVQPPSWDLHLCLPVFS
                     LDFFKILPHSLQYLPLAIPFGILTIIGGINNTESARLAGDAYRPRDILLTEAVTSLVS
                     AFFGGVAQTTPYIGHPAYKKMGATWRYTLATGLLVGVGSLVGLMSLAVSLVPRAVIAP
                     IFLFIGFEIVHQAYRESPPAHSPAVSLSMLPVIASLVTIILGQFLAALNIPANQLPER
                     LQQLHQTLNLLANGFILTGLLWGSLLAFLIDHKAKLAALCAAVCGVLTLFGVIHSVLP
                     TGELYLPWQAAGGAHYQLALAYGILSVILLILTGRTDLQKHSH"
BASE COUNT          601 a          769 c          795 g          516 t
ORIGIN      
        1 gttcttcgcg taatcgacga tgaacatgtc tttcatttca tcccagtaaa agtggtgctt
       61 gcgtccgaag aaacgcttgg ccacgtcctt ttcttccatt tcataaaaat gctcctgaat
      121 gagccggtcg tccacggttt tgacgggacg cagcgtgact ttctggccgg cgatggtcaa
      181 aacctcctcc agccaggccg gatatacgcc gaactgggat tcatacagtt ttcgttcctg
      241 gccgatcagg ccctcgcggc gcgcctgttc aaagaggctc tcccggaagt cggggtgggc
      301 cacgctgatc atggccatgg cgcgctcctg aacgtttttc ccgaacaggt tgacggcgcc
      361 gtattcggtg accacatacg tcacgtccgc cgccggaatg gcaacggtgc cggcggcctg
      421 ctccagaacg atgttgctcg acagcccgtc ttcgcttgcg gacgggacaa cgacaatgga
      481 tttcccgccc ggcgccatcg ccgcgccccg gctgaagtcc accagccccg ccacgtcgga
      541 aaaatggttt tgcgccgacc cgtcggccgc aacctggccc ttgaggtcaa tcgaggtcac
      601 gcgattgacg gccgtcatct tgtggtggcg ggcaatcatg accggattgc tcacataatc
      661 gcagggccgg aattcgatcg cgggatttcc attcagatac cggtaaagcc cctcgctgcc
      721 gatggcgccc gaagccacca tcttgccgtc gttgaatcct tttttccggt tggtaatgac
      781 gcctttttcc gcaagctttt tcatgacgtc cagaatcatc agcgagtgga tgcccagatt
      841 ttgcttttcc cccaaggcct ccagaaccag ctcctcggaa aaaccggggc tgatgtgcag
      901 cgtcgagccg tcttcaatca ggctggacaa gagcttggcg atccggtccg tgctggcgat
      961 tctgggcgtg gggtaaaccg acagcagatc ttcttcgtgt tcgacgataa aatcgacctc
     1021 atcgacgtgg atcatcccgt agcccggaac cacgggcatg ttcgggttga cctgaacgat
     1081 gacgacatcc gccgcgtggg cggccgccag cgtgatgtcc acggaaatgc ccaggttgag
     1141 ccacccgaat tcatcgggag gcgcggtctg aataagcgcg tagtgaatgg gaatatggcg
     1201 cttctgaaac aaagacggga ccgtgtagag attcatgggg gtcagaaaac gccgggaagc
     1261 cgtcagcccc cggatcatgc ccgatccctg atagatggag cgtacgtgat aattgcgccc
     1321 ttgtgtttct tccgcaacca agcccatgag agagccttcc aggttgagaa agcggaccac
     1381 ttccacatcc gagaaatggt cggtgtggtc cagaagcgcg gaaacgaggt gctgaggctc
     1441 tccgcagtac gaaccgatga acaccctttt tccccgggaa atggaacgaa tcgccgtttc
     1501 ggcggaaacc actttttccg aataggattt tttcatgctg cctccttcag tgggaatgtt
     1561 tttgtaaatc cgttcttccc gtcagaatca acaaaatgac cgacaggatt ccgtaggcca
     1621 gggcaagttg gtaatgcgct ccccctgccg cctgccaggg cagataaagc tctcccgtgg
     1681 gcagaaccga atgaatcacg ccaaaaagcg tcagcacgcc gcagacggcg gcgcacaggg
     1741 ccgcaagctt cgctttgtga tcgatcagaa aggcaagcag gctgccccag agaagcccgg
     1801 tcagaataaa gccgttcgcc agcagattca gcgtttgatg caactgctgc aaacgctccg
     1861 gcagttggtt ggctggaatg ttcagggcgg caagaaactg gccgagaatg atggtcacca
     1921 ggctggcgat aaccggcagc atgctcagac tcaccgcggg ggaatgagcc gggggagatt
     1981 cccggtaggc ctgatggacg atttcaaaac cgatgaaaag aaagatgggc gcgatgaccg
     2041 cccgcggcac cagactgacc gccagcgaca tcaacccgac cagagagccg acgccgacga
     2101 gcaaaccggt tgcaagcgtg tagcgccagg tcgcccccat ctttttatag gcgggatggc
     2161 cgatgtaggg ggttgtctgc gccacgccgc cgaaaaaggc ggaaacgagc gacgtgacgg
     2221 cttcggtgag aagaatgtcc cgggggcggt aagcgtcgcc ggccagacgg gcgctttccg
     2281 tgttgttgat tcctccgata atcgtcagga ttccaaaggg aatggccagt ggaaggtact
     2341 gaaggctgtg cggcaggatc ttgaaaaaat caagggaaaa taccggaaga cacaggtgca
     2401 gatcccagga gggcggctga acttcgggca ggcagccgct ccaccccagc gcaaagtgaa
     2461 ttcccgtgcc gacgagcagg gccgccacga cgccgtgaat gcgtccggga aattgaagcc
     2521 ggctgtagag cgcggcaaag atcagcccca gggcgaccag accgacgatc gcttcgttaa
     2581 agatctcgat catcggcaga aagcccaaaa gcatcagtcc gacgccgccg atggacccga
     2641 gcagaccggc ggtgggaatg ttgcgttgaa tccatccgcc g
//
LOCUS       DAOSIM010000268         2677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_33133_length_2677_cov_8.341724, whole genome shotgun sequence.
ACCESSION   DAOSIM010000268 DAOSIM010000000
VERSION     DAOSIM010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33133_length_2677_cov_8.341724"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="PLR47_09680"
     CDS             <1..829
                     /locus_tag="PLR47_09680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012236.2"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOG13260.1"
                     /translation="EGEELYREMEESGVLGNTVMVFGQMNEPPGARFRVGHAALTMAE
                     YFRDDAKQDVLLMIDNIFRFIQAGMEVSGLLGQLPSRLGYQPTLATELAELEERICNT
                     KTGAITSIQAVYVPADDFTDPSAVHTFGHLSATIALSRKRASEGLYPAIDLLQSGSKM
                     LMPNIAGERHYKIAQEIRKTLAVYEDLKDIIAMLGISELSREDQRTVFRARRLERFFT
                     QPFFVTEQFTGTAGKMVSREETLNGCERILNDEFAEYPERALYMIGPIEEAKIEHVA"
     gene            819..1199
                     /locus_tag="PLR47_09685"
     CDS             819..1199
                     /locus_tag="PLR47_09685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOG13261.1"
                     /translation="MLLKILLPAEVMMEREVKKIVAEAENGSFCLMPNHIDFVATLAP
                     GIFSFVPTEGGQELLAMDVGTLVKKGSDVLVSTRNAVKAAELGKLKQVVVEQYDALDE
                     REKLVRSAAAKLEASLIRRFVELK"
     gene            1257..1586
                     /locus_tag="PLR47_09690"
     CDS             1257..1586
                     /locus_tag="PLR47_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOG13262.1"
                     /translation="MVEIRRTTNRRRQMADHFAEKVAQREALRIKGLKHKNETVWFGL
                     GMFGIVGWAVAIPTLIGVALGLWIDRTWPSQYSWALMGLVAGVIVGCINAAYWVRKIR
                     SRIIEEE"
     gene            1588..1890
                     /locus_tag="PLR47_09695"
     CDS             1588..1890
                     /locus_tag="PLR47_09695"
                     /inference="COORDINATES: protein motif:HMM:NF024368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HOG13263.1"
                     /translation="MTPNALGMFVLMGAAGMALGALYFVGLWQTVKRLPQTEGRGLLL
                     LVSLVARLSVLLAIFYVLLQDGRWERLAAVLIGFVVMRKILTVRLGPQKTASAMQE"
     gene            1927..2655
                     /locus_tag="PLR47_09700"
     CDS             1927..2655
                     /locus_tag="PLR47_09700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOG13264.1"
                     /translation="MEIVALSQISPDQVVVWSWNFITLNVTILYTWLVMGILVGGSIW
                     VTRNLSSEMNVSRWQHFLEIVVSIVRGEISEMTKKGADNYVPLIGTLFLFICTSNVLA
                     VVPGYIAPTSSMTTTAALATCVFVAVPYYGISRNGVLHYVKEYFQPNFIFFPFHVMGE
                     LSRTLALTVRLFGNIMSHEKVIGILLAVTPFLFPVVMQILGLLIGVIQAYIFAILSMV
                     YIASALSAEDEDHHAEAKKEGSAA"
BASE COUNT          556 a          720 c          818 g          583 t
ORIGIN      
        1 cgaaggagag gagctctacc gggaaatgga ggaaagcggc gttctgggga acaccgtcat
       61 ggtgttcggc cagatgaacg aaccgccggg cgcgcggttt cgcgtcggac acgcggcgct
      121 gaccatggcc gagtatttcc gcgacgatgc caaacaggac gtgcttctga tgatcgacaa
      181 catcttccgc ttcatccagg cgggcatgga agtgtcgggc cttctggggc agctgccgtc
      241 ccggctcggg tatcagccga cgctggccac ggagctcgcg gagctcgagg agcgcatctg
      301 caacaccaag accggcgcaa tcacctccat tcaggccgtt tatgttccgg cggacgattt
      361 caccgatccc tcggccgtgc acaccttcgg tcacctgtcc gcgaccatcg cgctttcgcg
      421 caagcgcgcc agtgaaggct tgtatcccgc catcgatctg ctccagtcgg gatccaaaat
      481 gctgatgccc aacatcgccg gtgaacggca ctacaaaatc gcccaggaaa ttcgcaagac
      541 cctcgcggtg tatgaagacc tcaaggacat tatcgccatg ctgggcattt ccgaactgtc
      601 ccgcgaggat cagcgcaccg tgttccgcgc gcgccggctg gagcggtttt tcacgcagcc
      661 tttctttgtc accgagcagt tcaccggcac ggccggaaaa atggtttccc gggaagaaac
      721 gctcaacggg tgcgagcgga tcctgaacga tgagtttgcc gagtatccgg agcgcgcgct
      781 ttacatgatc ggaccgattg aagaggcgaa gatagaacat gttgcttaaa attttattgc
      841 cggcggaagt catgatggag cgggaggtca agaaaatcgt cgcggaagcg gaaaacggtt
      901 ctttctgcct gatgccgaac catatcgatt ttgtcgccac gctggccccg ggcatctttt
      961 cgtttgtgcc gaccgaaggc ggccaagaac ttctggccat ggatgtcggc acgctggtga
     1021 aaaagggatc ggatgttctg gtgtccacgc gcaatgccgt gaaggcggcg gaactgggaa
     1081 agctcaagca ggtggtggtg gaacagtatg acgcgctcga cgagcgggaa aaactggtcc
     1141 gctccgccgc ggcaaaactg gaggcgtcgc tgattcggcg cttcgtggaa ctgaagtagg
     1201 gcgcgatccc cgaacgcgcc gcacggaccc caaacgagca aggaaggaat ctcaacatgg
     1261 ttgaaatacg aagaaccacc aaccggcgcc ggcagatggc ggatcacttc gcggaaaaag
     1321 tcgcccagcg ggaggcgctg cgcatcaaag gcctcaagca taagaatgaa acggtctggt
     1381 tcggcctggg aatgttcggc atcgtcggat gggcggtggc gattcccacg ctgatcggcg
     1441 tggccctggg gctctggatc gaccggacct ggcccagcca gtattcctgg gcgctcatgg
     1501 ggctggtggc cggcgtgatc gtcggctgca tcaacgcggc ctattgggtc agaaaaatcc
     1561 gcagccggat catcgaagag gaatgacatg acgccgaacg ctttggggat gtttgttttg
     1621 atgggtgccg cgggaatggc gctgggcgct ctctattttg tcggtctgtg gcagaccgtg
     1681 aagaggctgc cgcaaacgga aggccgcggc cttcttctgc tggtcagcct ggtggcgcgc
     1741 ctgtccgtcc ttttggccat attctacgtt ttgcttcagg acggccgctg ggaaagactg
     1801 gccgcggtcc tgatcggttt cgtggtgatg cgcaaaatcc tgacggttcg tctgggtccg
     1861 caaaagacgg cgtcagcgat gcaggaatga cgaaaagggt tttgatcaac cggggaagga
     1921 gattctatgg aaatcgtcgc gttaagtcag atcagcccgg atcaggtggt tgtctggagc
     1981 tggaatttca tcacgctcaa tgtgaccatt ctctatacct ggctggtgat gggcattctg
     2041 gtgggcggtt ccatttgggt gacccgcaat ctttcttcag aaatgaatgt atcgcgctgg
     2101 cagcatttcc tggagatcgt ggtttccatc gtccgcggag aaatcagcga gatgacgaag
     2161 aagggggcgg acaattatgt tcccctgatc ggcacgctct ttttattcat ctgcacctcc
     2221 aatgtgctgg ccgtggtgcc gggatatatc gcgccgacgt cttccatgac gaccacggcg
     2281 gcgctggcca cctgcgtatt tgtcgccgtt ccgtactatg gaatttcccg aaacggcgtt
     2341 ttgcattatg taaaggagta ttttcagccg aactttattt tcttcccgtt ccacgtcatg
     2401 ggtgaactgt cccggacgct cgcgctcacc gttcgtcttt tcggcaacat catgagtcat
     2461 gaaaaggtca tcggaattct gctggccgtc acgccctttc tgtttcccgt cgtgatgcag
     2521 attctggggc ttctgatcgg cgtgattcag gcctacattt tcgccattct ttccatggta
     2581 tatattgcct cggcgctttc cgccgaggac gaagatcatc acgcggaagc caaaaaagag
     2641 ggctctgcag cctgataaaa ttgaaataaa ggaggag
//
LOCUS       DAOSIM010000269         2648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_33596_length_2648_cov_5.578866, whole genome shotgun sequence.
ACCESSION   DAOSIM010000269 DAOSIM010000000
VERSION     DAOSIM010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33596_length_2648_cov_5.578866"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2305)
                     /gene="dnaE"
                     /locus_tag="PLR47_09705"
     CDS             complement(<1..2305)
                     /gene="dnaE"
                     /locus_tag="PLR47_09705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOG13265.1"
                     /translation="MKQADFVHLHVHTQYSLLDGMIRLDDLFQKAKEYEMPAIAITDH
                     GTMFGAIDFYKQACAAGIKPIIGCELYVAPRGLADRTPSPAGETARHLVVWAKDMQGY
                     KNLMKLTSVAHLDGFYYRPRVDKALLKECSQGLIASSACLHGEIASHIVRGRMEDARR
                     AAREFQEIFGEDNFYLEVMENGIPEQKTANRGLMELGRELSIPLIATNDCHYLNADHA
                     EAHDVLLCIQTGRTIEDKDRMSMSSGQFYVRSSGEMQALFAEMPEAVSNTMDVANRCN
                     LTLEFGKFYLPKYEIKDPGESLEDHLDRKATQGLEKLFPVILKHWKEDEAAVRQKYTD
                     RLRSELEIIKKMGFAGYFLIVSDFIKHAKHTGVPVGPGRGSAAGSLVAYAIRITDIDP
                     LLYGLFFERFLNPDRISMPDIDIDFCQERRGEIIKYVTEKYGKDRVSQICTFGKMMAK
                     GVIRDVGRALNIPYGEVDRIAKLVPTFPLNIKLADAFKAEPRFEEERRKNPKIDKMLS
                     LSLVLEGLNRHSSVHAAGIVISDVPLVERVPLFSPKKDDRVSQYSMKDIEAVGLTKFD
                     FLGLKTLTVIKNAVDFIRDSHHVDVDVNNLPLDDEETYRLLAKGETDGVFQLESAGMK
                     DLLVNFKPDRIEDVITLIAAYRPGPMKMIPDFIARKHGKQQIAYELPQLEPILKETYG
                     IILYQEQVMQIANVIGGYTMAQADTLRKVMGKKQVAAMEKEKPRFLEGAKKKTINENK
                     AKIIWNQMEDFAEYGFNKSHSAAYAMIT"
     gene            complement(2310..>2648)
                     /locus_tag="PLR47_09710"
     CDS             complement(2310..>2648)
                     /locus_tag="PLR47_09710"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOG13266.1"
                     /translation="FQGRSYKVYRGMGSLEAMKMGSRDRYYQDDVEAPSKTVPEGIEG
                     RVPFRGTLSASIVQLIGGLKAGMGYVGCRTIAELIEKSRFVRITSAGLRESHVHDVII
                     TKEAPNYWID"
BASE COUNT          525 a          741 c          728 g          654 t
ORIGIN      
        1 aggtgatcat cgcgtaggcg gcgctgtgag acttgttgaa accgtattcg gcgaagtctt
       61 ccatctgatt ccatatgatt tttgccttgt tctcgttgat cgttttcttt ttcgcgcctt
      121 caagaaaacg gggtttctcc ttttccatcg ccgctacctg ctttttcccc atcactttgc
      181 gcagcgtgtc ggcctgcgcc atggtatatc ctccaatgac gttggcgatc tgcatgacct
      241 gttcctggta aaggatgatg ccgtaggttt ctttgaggat cggctccagc tggggaagct
      301 cataggcaat ctgctgcttt ccatgcttgc gggcgataaa atcgggaatc atcttcatcg
      361 gtcccggccg gtaggcagca atcagcgtga tgacgtcttc gatgcggtcc ggcttgaaat
      421 tgaccagcag gtctttcatc ccggcgcttt ccagctggaa aacaccgtcg gtttccccct
      481 ttgcgagcag acggtaggtt tcttcatcat cgagaggaag attgttcacg tcaacatcga
      541 catggtggga atcccggatg aaatccacgg cgttcttaat gaccgtcagc gtcttgagac
      601 ccaaaaaatc aaatttggtc agcccgacgg cttcgatatc cttcatcgaa tactgggaaa
      661 ccctgtcatc ttttttggga gaaaaaagcg gaacacgctc gaccagcgga acgtcggata
      721 tcacaatgcc ggcggcgtgc acggaagaat gccggttgag gccttccaga accagcgaca
      781 gcgacagcat tttgtcgatc ttcgggttct tcctgcgttc ttcctcaaaa cgcggctccg
      841 ccttgaacgc atccgctagc ttgatgttca gcggaaaggt cggcaccagt ttggcaattc
      901 ggtccacttc gccataggga atgttcaggg ctctgcccac atcccgaatc acgcccttgg
      961 ccatcatctt gccgaaggtg cagatctggg agactctgtc cttgccgtat ttttccgtga
     1021 cgtatttgat gatttcaccg cggcgttcct ggcagaaatc gatgtcgatg tccggcatgc
     1081 tgatccggtc cggattgaga aaacgctcga aaaacaatcc gtagagcaag ggatcgatgt
     1141 cggtgatgcg aatggcatat gccaccagcg atccggcagc ggagccgcgt cccggaccga
     1201 cgggcacccc cgtgtgcttg gcgtgcttga taaaatcgga aacgatcaga aaatatccgg
     1261 caaaccccat ttttttgatg atttccagct ctgagcgcag gcgatccgtg tatttttgcc
     1321 gaacggccgc ctcgtcctct ttccagtgct tcaaaatcac cgggaacagt ttttccaggc
     1381 cctgggtcgc cttgcgatcc agatgatcct caaggctttc tccggggtcc ttgatctcgt
     1441 atttcggcag gtagaatttc ccgaattcca gggtcaggtt gcatcgattc gcgacatcca
     1501 tcgtattgga gactgcttcg ggcatttcgg caaaaagcgc ctgcatttcc ccggaggaac
     1561 ggacgtaaaa ctggcccgac gacatggaca tccggtcttt gtcttcgatg gttcttcccg
     1621 tctgaatgca cagcaaaaca tcgtgcgctt ccgcgtgatc tgcgttcaga tagtgacagt
     1681 cgttcgtggc gatgagcgga atggagagct ccctgccaag ctccatcaag ccccggttgg
     1741 cggttttctg ctcgggaatg ccgttttcca tgacctccag atagaaattg tcctcgccga
     1801 aaatttcctg aaactcacgg gcggccctgc gggcgtcttc catgcggccc cggacgatat
     1861 gcgaagcgat ttccccgtgg aggcatgcgc ttgacgcgat cagaccttgg gagcattctt
     1921 tcagcagggc cttgtccacg cgcggccggt agtaaaatcc atccagatgg gccacggaag
     1981 tcagcttcat cagatttttg tagccctgca tgtctttggc ccaaaccacc agatggcgcg
     2041 ccgtttcacc ggcgggcgac ggggttctgt ccgcgagccc gcggggcgcg acataaagtt
     2101 cgcagccgat gatcggcttg atgccggccg cgcaggcctg cttgtagaag tcgatcgcgc
     2161 cgaacatcgt gccgtggtcg gtgatggcga tcgccggcat ctcgtattct ttggcctttt
     2221 gaaagaggtc gtccaggcgg atcatgccgt cgagaaggct gtactgcgta tggacgtgga
     2281 gatggacgaa atcggcttgc ttcatttggc taatctatcc agtaattggg cgcttccttg
     2341 gtgatgatca cgtcgtgaac gtggctttcc ctgaggccgg ccgacgtaat gcggacgaac
     2401 cgggattttt cgatcagttc cgcaatggtc cggcatccga cgtagcccat gccggctttc
     2461 agcccgccga tcagttggac gatgctggcg gaaagcgtgc cgcggaaagg caccctgccc
     2521 tcgatccctt ccggaaccgt cttggaaggc gcttcgacgt cgtcctgata gtagcggtcg
     2581 cggcttccca ttttcatggc ctccagcgat cccatgccgc gatatacctt gtaactgcgc
     2641 ccctgaaa
//
LOCUS       DAOSIM010000270         2647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_33608_length_2647_cov_7.008873, whole genome shotgun sequence.
ACCESSION   DAOSIM010000270 DAOSIM010000000
VERSION     DAOSIM010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33608_length_2647_cov_7.008873"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PLR47_09715"
     CDS             complement(<1..634)
                     /locus_tag="PLR47_09715"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOG13267.1"
                     /translation="MNPEDAKYWLALKSLEGVGNTLVGPLLERFGSPASVFSAPVHEL
                     AGVRGMSRKIAEAILAFSDWDGILRQIDGLNQAGFRVVTFLDECYPKNLLNMYDRPAL
                     LYVAGVLNSDDISIAIVGSRHASTYGRYTTDRISRELALRGVTIVSGMARGIDSCAHR
                     GALAAKGRTIAVLGSGLDVIYPPENKKLFDAIVQQGAVISEYPPGTQPLPF"
     gene            802..2520
                     /locus_tag="PLR47_09720"
     CDS             802..2520
                     /locus_tag="PLR47_09720"
                     /inference="COORDINATES: protein motif:HMM:NF040132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13268.1"
                     /translation="MNKNIVFFICATLMFCSFSAFALTPSDPKGTVRPADGAAASKNL
                     HPEEAHPVLTIMPREVDLGFIQPGSTAFGEFKLSDITPGTVESAASCPDGWEALSGKA
                     LPLKVSHEPVSLRLELTVADTPEKARIDKTKDPAYAVRMKMEAAGRELICRKSLKPGH
                     HRKDIPLTLAGGARNIFVEFRIQTAQGIPSISLYPQRLDLGTQSPGKILSKKIELANR
                     SREKLHWSVSSHPEAGKSAPPPEPKQERYFSFRQEELPEPGKYVIPEHLKDAMELTGK
                     WTERNGYPVSRGGGALKYRFQGTGISIFLQSRTPEGNCVVYLDEAPVSLPDELSGESE
                     KEEILIAEGLADGPHTLNLVIRGGALELEGVKVFGREVQRGPKGWITVFPNSGTTLSE
                     IDYLNVKVDTTSLAPGIYGDDIVFQTNAGEETAEVYVSIVPDAGHKVLDVYLYSSDYD
                     YLYTSDPQTEARRLYQNRYVKEGIAFRLFAPQTPGTTAFHRWYNPAIKDHFYSHDRTG
                     GGKRLDGYIYEGNIGNIATSRMTNTRELYRWYNPSTGRHYYSTQPKVPPALKKYRFDG
                     IAGYVR"
BASE COUNT          637 a          746 c          716 g          548 t
ORIGIN      
        1 gaaacggcag gggctgcgtc cccggcggat attcggagat gacggcgccc tgctgcacaa
       61 tcgcgtcgaa cagctttttg ttttccggag ggtagattac atccaggccg ctccccagaa
      121 cggcaatcgt tctgcccttt gccgccagcg cgccgcggtg cgcacaggaa tcgattcccc
      181 gcgccatgcc gctgacaatg gtgacacccc ggagcgcaag ttcccggctg attctgtccg
      241 tggtgtaccg tccataagtg ctggcatgcc gggacccgac aatggcaatg gaaatgtcgt
      301 cgctgttcaa aacgcccgca acgtaaagca gggcagggcg gtcatacata ttcaaaagat
      361 ttttaggata gcattcgtcc agaaaagtca ccacccggaa gcccgcctga ttcagaccgt
      421 cgatctggcg cagaatgccg tcccagtcgc taaacgcgag aatcgcttcc gcgatctttc
      481 tgctcatgcc gcggactccc gccagttcgt gaaccggagc cgaaaagacg gaggccgggg
      541 agccgaaacg ctccagcagg gggccaacga gcgtgttgcc gacgccctcc agggatttca
      601 gcgccagcca gtattttgcg tcttcaggat tcataggcaa tgacgacaga gtgttggtat
      661 gcaaatttat ggtgccggaa aattaggctg aacattacat cttctcgatt tactctgtca
      721 agtattttaa aatgatttaa aatggttgcg taaaatgctt ctttacgcta aaaaagcgca
      781 acctattcac cagaggaatc catgaataaa aatattgttt ttttcatttg cgcaactttg
      841 atgttttgct cgttttccgc tttcgcgctg acgccttcgg acccgaaagg gaccgtcagg
      901 ccggccgacg gagctgccgc ttcaaagaac cttcatccgg aggaggcgca tcccgtcctg
      961 acgattatgc ccagggaagt ggacctggga ttcatccagc cgggctcaac cgcttttggt
     1021 gaattcaagc tcagcgacat cacgccggga accgtggaat ccgcggcatc ctgcccggac
     1081 ggctgggaag ctctttccgg aaaagcgctt ccgctgaaag tctcccatga accggtttct
     1141 ttgcggctgg aattgacggt cgccgacact cctgaaaaag cgcgcatcga taaaaccaaa
     1201 gaccccgcct atgcggtgag gatgaaaatg gaggccgccg gcagggagtt gatctgccgc
     1261 aaaagtctga aacccggaca tcaccggaag gacattccgc ttaccctggc cggcggtgca
     1321 agaaatattt ttgtggaatt ccggattcag accgcacagg gaattccgtc catcagcttg
     1381 tatccccagc gtttggacct tggcactcag tcgcccggga aaattctgtc caaaaagata
     1441 gagctggcca acagaagcag ggaaaagctt cactggtccg tctccagcca cccggaagcc
     1501 ggaaagtccg cgcctccccc agaacccaaa caggaaaggt acttttcttt ccgccaggag
     1561 gaattgccgg agcccggaaa atacgtcatt cccgagcacc tcaaggacgc catggagctc
     1621 accggaaaat ggaccgaaag aaacggctac cccgtaagcc ggggcggcgg cgcgctgaaa
     1681 taccgctttc agggaaccgg catcagcatt tttctgcagt ctcgcacgcc ggaaggaaat
     1741 tgcgttgttt atctggacga agccccggtg agcctgccgg acgaactgtc cggggagtcg
     1801 gaaaaagagg aaattctaat cgcggaaggc ctggccgacg gcccccacac ccttaacctg
     1861 gtcatccggg ggggcgctct ggaactggaa ggcgtgaaag tattcggccg ggaagtacag
     1921 cgggggccga agggctggat cacggttttt cccaattccg gcacaacgct gagcgaaatc
     1981 gattatctca acgtcaaggt ggatactacc tctcttgcgc ccggcattta cggcgacgat
     2041 atcgtctttc aaaccaacgc cggggaggag accgcggagg tgtatgtcag catcgttccc
     2101 gacgcgggtc ataaagtcct ggatgtctat ttatattcca gtgattatga ctatctctac
     2161 acatcggacc cccagacgga agcccggcgt ctttaccaga accgatacgt caaggaaggc
     2221 attgcctttc ggcttttcgc gccgcaaact cccggcacga ccgcctttca ccgctggtac
     2281 aatcccgcca taaaggacca tttttattcc cacgaccgga ccggcggggg gaaaaggctg
     2341 gatggatata tttatgaagg gaacattggc aacatcgcca cgtcccgcat gaccaatacc
     2401 cgcgaattgt accgctggta caatccgtcc accggccggc actactattc gacccagccg
     2461 aaagtgccgc ccgccctgaa gaaataccgc tttgacggca tcgccgggta tgtccggtag
     2521 aataaaagat tcaccctttt taaccttgac aaaaaaaaga agaggggata ttaggcgttc
     2581 tctttttggt gcaggattaa atgcgcctgt agctcagctg gatagagcaa cggacttcta
     2641 atccgtg
//
LOCUS       DAOSIM010000271         2614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_34175_length_2614_cov_7.526377, whole genome shotgun sequence.
ACCESSION   DAOSIM010000271 DAOSIM010000000
VERSION     DAOSIM010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34175_length_2614_cov_7.526377"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PLR47_09725"
     CDS             complement(<1..567)
                     /locus_tag="PLR47_09725"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOG13269.1"
                     /translation="MQVKDFSYSLPEELIAQHPRPQRDASRMMKIDRGAKTIEDDLFA
                     ALPDYLEPDDVLVINDSRVIPARIYGKKATGAILELLLLTRRETNGEAQSWEVMARPA
                     KRMKEQDVIDLGHSCEALVVSRISDKKWLMTFCAKGGFENYLERFGRAPLPPYIKRKK
                     SADPDQTDDRRRYQTIYAKTPGSVAAPTA"
     gene            complement(637..822)
                     /locus_tag="PLR47_09730"
     CDS             complement(637..822)
                     /locus_tag="PLR47_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HOG13270.1"
                     /translation="MDYFLCVMGMVFIVEGLPYLVFPEKMKIYLVKISELPENTLRIT
                     GICAIALGLILLYFGRR"
     gene            complement(826..1638)
                     /gene="mazG"
                     /locus_tag="PLR47_09735"
     CDS             complement(826..1638)
                     /gene="mazG"
                     /locus_tag="PLR47_09735"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOG13271.1"
                     /translation="MAPSIVIMNQTQTQLQELVALIQRLRAPDGCPWDRKQTRQDIGK
                     YLLDECYEVMDALAEDDPAHIQEELGDLLFQILFIAEISAEAGEFSLSDVMQGIREKM
                     IRRHPHVFGDVKVESVQDVKDNWQEIKKKERGGQAPGENPFGGIPRSVPALKRAHKIS
                     SAASRLGFDWPDANAVLDKLKEELVELEEARKQGNEFQIEEEMGDLFFTLVNLGRFLN
                     VDAEKAAAGAADKFVRRWSYMTQRLAALGRSPSEATQAELDSIWNEAKQKGI"
     gene            complement(1620..2018)
                     /locus_tag="PLR47_09740"
     CDS             complement(1620..2018)
                     /locus_tag="PLR47_09740"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOG13272.1"
                     /translation="MSDQDIRERTQKTAEKLFSGGAKLERPYSLWREFDRGLANDFSR
                     FITGNLYSREVLTIQERQMAACAMLAALKARDELKVHANAALNVGCDPKKLVEIFFQT
                     ATYAGMPAVNEALAAFREVLEARGEWPLPS"
     gene            complement(2032..>2614)
                     /locus_tag="PLR47_09745"
     CDS             complement(2032..>2614)
                     /locus_tag="PLR47_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOG13273.1"
                     /translation="SDPITNMSFSAQGYALLNEKLAPDIAVLEGGYSIEKALPYVNVG
                     IILAMAGLDFSQVREPDYDAQRIRQSPEITRLIEEEARNVMTLFKKKKNLKKQVTGSA
                     AYIRREKNIYYDTDNIWEKQTETVRVCDQCGGVVMIDSAADTGKRIYAVILPNSCCPE
                     CRENGENFFARMNSSQYNHVYFQDRQRDVFIVK"
BASE COUNT          501 a          758 c          730 g          625 t
ORIGIN      
        1 ggcggtcggc gcggcgacgg agccgggcgt tttcgcgtaa atggtctggt agcgccggcg
       61 gtcgtccgtt tgatccggat ccgcgctctt ttttcttttg atgtagggag gaagaggcgc
      121 ccgcccgaaa cgttccaggt agttttcaaa accgcctttt gcgcaaaagg tcatcagcca
      181 ttttttgtcg gaaatcctgg agacgaccag cgcctcgcag gaatgcccga ggtcgatgac
      241 gtcctgttct ttcatcctct tggccggccg ggccataacc tcccatgact gggcctcgcc
      301 gttcgtttct ctgcgtgtga ggagcaagag ctccaggatc gcgccggtcg cctttttgcc
      361 gtagattctg gccggaatga ctcgggaatc attgatcacg agaacatcgt ctggttccag
      421 atagtccggc agggcggcaa acaggtcatc ctcgatggtt ttggcgccgc gatcaatttt
      481 catcatgcgc gaggcgtccc tttgcggacg cgggtgctgc gcgatgagct cttcgggaag
      541 agagtatgaa aaatccttta cctgcatcgt ttccttcaag agtcgaaaaa gttcaggcaa
      601 ataatcagaa tccgccgggg ctgtcaatga aaaccgtcag cgccttccga aataaagcag
      661 aatcaggccc agcgctatgg cgcaaatgcc cgtgatgcgc agcgtgtttt ccggcagctc
      721 cgagatcttg accagataga ttttcatttt ttccgggaaa accagatagg gaaggccttc
      781 aacgataaaa accatgccca tgacgcaaag aaaataatcc aatcctcaga ttcccttctg
      841 tttggcttcg ttccagatcg aatccagctc ggcttgcgtt gcctcagaag gcgagcggcc
      901 gagggcggca agcctctgtg tcatgtaaga ccagcgcctc acaaatttat ccgcggcgcc
      961 ggcggccgcc ttttcggcgt ccacgttgag aaagcggccg agattcacca gcgtgaaaaa
     1021 gagatcgccc atttcttctt cgatttgaaa ttcgtttccc tgtttccgcg cttcctcaag
     1081 ctcgaccagt tcttctttta atttgtccag aaccgcgttc gcgtccggcc agtcgaagcc
     1141 gaggcgggag gcggcggacg atattttatg cgcccgcttc agcgccggca ccgaacgggg
     1201 aatgccgccg aaaggattct cgccgggcgc ctggccgccg cgctccttct ttttgatttc
     1261 ctgccagttg tcctttacat cctgtacgga ttcaaccttc acgtcgccga aaacatgcgg
     1321 atgccgccgg atcatttttt cccgaatgcc ctgcatgacg tccgacaggg aaaactcgcc
     1381 cgcttcggcc gaaatttcgg cgataaacaa tatctggaaa agcagatcgc ccagctcttc
     1441 ctgaatgtgc gccggatcat cttcggccag cgcgtccatc acctcatagc attcgtccag
     1501 cagatatttt ccgatgtcct gccttgtctg cttccggtcc caggggcagc cgtccggcgc
     1561 gcgcagtctt tggatcagcg cgacaagctc ctgcagttgt gtttgggtct ggttcatgat
     1621 cacgatggaa ggggccactc gccgcgcgcc tcaaggactt ccctgaaagc ggccagcgct
     1681 tcattgacgg ccggcatgcc ggcgtaggtt gccgtctgaa agaaaatttc gaccagtttc
     1741 ttcggatcgc agcccacgtt cagggcggcg ttggcgtgga ccttcagctc gtcccgggcc
     1801 ttgagcgcgg ccagcatcgc gcaggcggcc atctggcgct cctgaatcgt caggacctcg
     1861 cgcgaataga ggttccccgt gatgaagcgc gaaaaatcat tggccagccc gcggtcaaac
     1921 tcgcgccaca gggaataggg gcgctccagt ttggcgccgc cggaaaaaag tttttccgcc
     1981 gttttctgcg ttctttccct gatatcctga tccgacatgt tgcctcccgg gctatttgac
     2041 gatgaaaacg tctctctgcc tgtcctgaaa ataaacatgg ttgtactgcg aactgttcat
     2101 gcgggcgaaa aagttctccc cgttttcgcg gcattcgggg cagcaactgt tgggcagaat
     2161 gaccgcgtag attctcttgc ccgtgtcggc ggccgagtcg atcataacca cccctccgca
     2221 ttggtcgcag acccggacgg tttcggtttg cttctcccag atgttgtccg tgtcgtagta
     2281 aatgttcttt tcacggcgaa tgtaggcggc ggagccggtg acctgcttct tcaggttttt
     2341 ctttttcttg aacagggtca taacattccg ggcttcctcc tcgatcagcc gggtaatttc
     2401 cggcgactgg cggatgcgct gggcgtcgta atccggctcg cgaacctggc tgaagtccag
     2461 tccggccatc gccagaatga tccccacgtt gacgtagggc agggcctttt caatggagta
     2521 gcctccttcc agcacggcaa tgtcgggcgc gagtttttcg ttgagcagcg cgtacccctg
     2581 ggcgctgaag ctcatgttcg tgatcgggtc gctg
//
LOCUS       DAOSIM010000272         2613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_34190_length_2613_cov_7.393276, whole genome shotgun sequence.
ACCESSION   DAOSIM010000272 DAOSIM010000000
VERSION     DAOSIM010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34190_length_2613_cov_7.393276"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="PLR47_09750"
     CDS             <1..1378
                     /locus_tag="PLR47_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOG13274.1"
                     /translation="VFGMALPAFAADMMQFLKENGFADQSKDVLTIPSAALEKRADVL
                     RRRLAALDACVKGLKEEKGEALRRMNADYRAYEEVLKAMRLSVISSRAMFLTGWMDAK
                     EKTILMEIMKEVCGGGFIVSEKKDANAPVRLKNLRLFRPFELLVKTMGMPSSNELDPT
                     PLTAITYVMMFGLMFGDLGQGIVLMLCGWLLKKFGRKKAREELAQAGGILLACGGSAA
                     VCGLLYGSVFSSEHLLPALWFHPTAQIMRLFFFTIMMGVVFILVGLCLNIINSFLNAD
                     YSEAFLEKRGLAILFVYAAVVVFVLLYQKTGEPPALWALGVFIFLPLMIFSLRGLLGV
                     LFMEKPKPHSISEYIVETIMEVVEIALGLFANTISFIRVGAFALSHAGLSIVTYTLAG
                     MADPSLKSAGAIIIIVCGNIFIIGFEGLICGIQSMRLEYYEFFSKFFRGDGVVFSPFT
                     LPAKASEV"
     gene            1380..1712
                     /locus_tag="PLR47_09755"
     CDS             1380..1712
                     /locus_tag="PLR47_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HOG13275.1"
                     /translation="MTRSRQLAFLMGAIFLCALWSLFPAMGWASEGVPAKAFTDAIGL
                     AYIGAGVAVGFACLASGYAVAKIGSAAIGAVSEKPELMGRTLVFLGLAEGIAIYGLII
                     AIMILNKL"
     gene            1745..1972
                     /locus_tag="PLR47_09760"
     CDS             1745..1972
                     /locus_tag="PLR47_09760"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOG13276.1"
                     /translation="MPSRAAAALERIVGKKDAGIVLITSALASELGGRITEINLGRPS
                     PVVVEIPGIDDPEGFRGSVMAYVQEALGISL"
     gene            2133..>2613
                     /locus_tag="PLR47_09765"
     CDS             2133..>2613
                     /locus_tag="PLR47_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13277.1"
                     /translation="MDNTTLEDSIRRASEEMIAGIREKEARELERLDEDCAAEIERFR
                     KKTQEETQARMDQELSRLENRAVLERKKLKLTTVERFIHRIVDDVMKGIRSHPRYRQF
                     LVDAAAGAAAEIPGNVEVRLAPNDGVYGNEIDEALRAAGGDRQIRIEADAGIGWGGCL
                     "
BASE COUNT          522 a          678 c          835 g          578 t
ORIGIN      
        1 tgtgttcggg atggccctgc cggcttttgc cgccgacatg atgcagtttc tgaaagaaaa
       61 cggatttgcc gaccaatcga aggatgtcct gaccattccc tccgcggcgc tggaaaaacg
      121 cgcggacgtt ctgcggcggc gtcttgccgc gctggatgcc tgcgtcaagg gcctgaaaga
      181 ggaaaaagga gaggccctcc ggcggatgaa cgccgattac cgggcctatg aggaagtgct
      241 caaagccatg cgcctgtccg tgatttcatc gcgggccatg tttctcacgg ggtggatgga
      301 cgcaaaggaa aagacaatcc tcatggaaat catgaaggag gtttgcggcg gcgggtttat
      361 cgtgtccgaa aagaaggacg ccaacgcccc ggtccggttg aaaaacctgc ggctgttccg
      421 gccctttgaa ctgcttgtca aaaccatggg aatgccctcc tcaaacgagc tggatcccac
      481 gccgctgacg gccattacct atgtcatgat gttcggactg atgttcggcg atcttgggca
      541 gggcatcgtc ctgatgctgt gcggctggct gctgaaaaag ttcggccgga agaaagcccg
      601 cgaagaactg gcgcaggcgg gaggcattct tctggcctgc ggcggctcgg cggccgtctg
      661 cggtctgctt tacggcagcg tcttttccag cgagcacctc ctgcccgctc tgtggtttca
      721 ccccacggct cagatcatgc gcctgttttt cttcaccatc atgatgggcg tggtgtttat
      781 ccttgtcggc ctttgcctca acatcatcaa cagttttttg aatgcggatt attccgaagc
      841 ctttctggag aagcggggac tggccatcct cttcgtttac gcggctgttg tcgtattcgt
      901 cctgctctac cagaagaccg gcgagccccc cgccttatgg gccctggggg tttttatctt
      961 ccttccgctg atgatctttt cgctccgagg cctgctgggc gtgctgttta tggaaaaacc
     1021 caaaccgcac agcatttcgg aatacatcgt tgaaacgatc atggaggtgg tggagattgc
     1081 gctgggtctt tttgccaata cgatctcttt cattcgcgtg ggcgcctttg ctctttcgca
     1141 cgcgggactg agcattgtca cctatacgct cgcggggatg gccgacccgt ccctgaaatc
     1201 cgcgggcgcc atcatcatca tcgtgtgcgg caatattttt atcatcggtt tcgaaggtct
     1261 gatttgcggc attcagtcca tgcgtctcga atattacgag tttttcagca agtttttcag
     1321 aggagacggg gtggtctttt ccccgtttac actaccggca aaagcatcgg aggtgtgaga
     1381 tgaccaggag caggcagttg gcgtttctga tgggggcgat tttcctgtgc gccctgtgga
     1441 gtctctttcc ggccatggga tgggcgtccg agggagtccc ggccaaagcg tttacggacg
     1501 caatcgggct ggcctacatc ggcgcggggg tggccgtggg gtttgcctgt ctggccagcg
     1561 gctacgcggt ggccaagatc ggctccgcgg ccatcggtgc ggtgagcgaa aaaccggagc
     1621 tgatggggcg cactctggta tttctggggt tggcggaagg cattgccatt tacggcctga
     1681 ttatcgccat catgattctg aataagctgt aatatggaaa aaatttacat gatcggagac
     1741 atggataccg tcacgggccg ccgccgcgct cgaaaggatc gtcgggaaaa aagacgcggg
     1801 aatcgtgctc atcaccagcg cgctggcgtc ggagctcggc gggcgcatta cggagatcaa
     1861 cctcggcagg ccgtccccgg tggtggtcga gattcccggc atcgacgacc cggaagggtt
     1921 tcgcggatcc gtgatggcgt acgtgcagga ggccctggga atatcattgt gagcatcatc
     1981 gccggagcat tgtagggaac ggtcgcgacc gttccttgtg gggttacgcg ggatgaataa
     2041 tagaaaaaac tcatggctca tggaatgcag ggaaaggtct tgccgcccgc aagggtggtg
     2101 gtgaccgttc cctcacgaca gaaggattgg ctatggataa cacaacactc gaagattcca
     2161 ttcggcgggc gtcggaagaa atgattgccg gaatccgcga gaaggaagcc cgcgagcttg
     2221 agcggctgga cgaggattgc gcggccgaga ttgagcgttt ccgcaaaaag acacaagaag
     2281 aaacgcaggc gcgcatggat caggagcttt cccggctgga aaaccgggcc gttctggaga
     2341 ggaaaaagct caagctgacc acggtggagc gattcattca ccggatcgtc gatgatgtaa
     2401 tgaagggaat tcggagccat ccgcggtatc ggcaatttct cgtggatgcg gcagcgggcg
     2461 ccgccgcgga aattccgggg aatgtggaag tgcggcttgc gccgaacgac ggggtttacg
     2521 gaaacgagat tgacgaggct ctccgcgcgg cggggggcga tcggcagatt cgcattgagg
     2581 ccgatgccgg catcggttgg ggcggctgcc tgg
//
LOCUS       DAOSIM010000273         2612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_34212_length_2612_cov_9.483379, whole genome shotgun sequence.
ACCESSION   DAOSIM010000273 DAOSIM010000000
VERSION     DAOSIM010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34212_length_2612_cov_9.483379"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /locus_tag="PLR47_09770"
     CDS             complement(<1..1599)
                     /locus_tag="PLR47_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOG13278.1"
                     /translation="MFPKQANDVSILCIDDDPDVLNLLKKILTGAGYSVNVAADGKQG
                     IAEALSRQPDLILMDVMMPEIDGYVACFELQANPETKFIPVIFLTALDDEVNRAKAFA
                     VGAADHLSKPIRKDVLLRKIDEQIKTSSRWQKLKTRIAAKDEVVQPPAIFSQFKQSLL
                     NFVEPAQAARIRDRLSALAPQDIYNICEPMGIPQRQMTEWISEFLSIPVIAKIDRESL
                     LLGVIPVSFARENRVVARLDENQQRSFILCNPFDWSLMDALKKLFGLKSNAGLNLAAP
                     EDIDALFQQSDVESLVKLPGEEQAESAERMLAIDDAEQQEDEGEITQEINSEIIALVN
                     RILVDAYNKRASDIHFDSGFRGQPFKVRFRIDGVCQSAYFIPEKSKRAIISRIKIISN
                     LDISERRKPQSGKFMVRVRGEKVEFRVEVTPTVENNENIVLRILAKSKPVAIHQLGLS
                     PHNLEAFESMITKPQGVVLCVGPTGSGKTTTLHSALGHINKPEITIWTAEDPVEIQQA
                     GLKQVQINSKIGFTFSEALRSFLRA"
     gene            1890..>2612
                     /locus_tag="PLR47_09775"
     CDS             1890..>2612
                     /locus_tag="PLR47_09775"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG13279.1"
                     /translation="MDFWLKDEQKMLRYNIREFAEQEIAPVAMELDEKEEFSCDLTRK
                     MAKLGLFGIYVPAEFGGAGMDYLSYIIAVEEICRIDGSHGATVAAGNSLGIGPIYYYG
                     SKKQKNEWLPRLCAGEILASFGLTEPNAGSDAGASKTNARLENGQWVVNGSKIFITNS
                     TSKMAGICTVQAITGTKGQKVGPYGSIETKEVSCILVPNGTPGFTTKKMTKKMMWRSS
                     DTGELYFDDCRVPEENLLGKRGE"
BASE COUNT          592 a          702 c          719 g          599 t
ORIGIN      
        1 ggcccgcagg aaagaccgga gcgcttcgct gaacgtgaag ccgattttgc tgttgatctg
       61 cacctgcttc agtccggcct gctggatttc cacgggatct tccgccgtcc agatggtaat
      121 ctccggttta ttgatgtgcc cgagagccga gtgcagcgtg gtcgtttttc cggacccggt
      181 gggtccgacg cacagcacga cgccctgcgg cttcgtaatc atgctttcaa aagcttccag
      241 attgtgggga gaaagcccga gctgatggat ggccaccggc tttgacttgg ccagaattct
      301 cagaacaata ttttcattat tttccaccgt gggcgtcact tccacgcgga attccacttt
      361 ctcgccgcga acgcgcacca tgaacttgcc gctctgcggc tttcggcgtt ccgaaatatc
      421 cagattggag atgatcttga ttctcgagat gatggctctt ttggactttt ccggaatgaa
      481 atacgccgac tgacaaacgc cgtcgatgcg gaatcggacc ttgaacggct gtccccgaaa
      541 gccggaatca aaatgaatgt ccgatgcccg cttgttatag gcgtccacga ggatccggtt
      601 gacgagggca atgatttcgg agttgatttc ctgggtaatt tccccttcgt cttcctgctg
      661 ttcggcatcg tcgatggcca gcattctttc ggctgactcg gcctgctctt ctccgggaag
      721 cttcaccaga gactcgacat ccgactgctg gaaaagggca tcaatgtctt caggcgccgc
      781 cagattcagg cccgcgttgc ttttcaggcc gaacagtttc ttgagcgcgt ccatcaggct
      841 ccagtcaaag gggttgcaca gaatgaacga tctttgctga ttttcgtcca gccttgccac
      901 aacgcggttt tccctggcga aagaaacggg aatgaccccg agaagaaggc tttcccggtc
      961 aattttggcg atcacgggga tggacagaaa ttctgaaatc cactccgtca tctgccgctg
     1021 gggaatgccc atcggctcgc agatattgta aatgtcctgg ggcgccagcg cggacagacg
     1081 gtcccgaatg cgggcggcct gtgcaggctc tacaaagttc agcaaggatt gcttgaactg
     1141 ggaaaaaatg gccggcggct gcacgacttc atccttggcg gcgatcctgg ttttcagctt
     1201 ctgccagcgg gagctcgtct tgatctgctc gtcgatcttg cgaagcaaaa cgtcttttcg
     1261 gatcggcttg gacaggtggt ccgccgcgcc caccgcaaag gccttggccc gattgacttc
     1321 atcgtcaagg gccgtgagaa agatcacggg gatgaacttg gtttccggat tggcctgcag
     1381 ttcaaaacag gccacgtagc cgtcgatctc cggcatcatc acgtccatca gaatcagatc
     1441 cggctgccgg ctgagggctt ccgcgattcc ctgcttgccg tccgcggcca cgttgaccga
     1501 atagcctgcc ccggtcagaa tctttttgag cagattcaga acatccggat cgtcatcaat
     1561 gcacaggatg gaaacatcat tggcttgttt tggaaacatt ttgcctcctt gcagagcgtt
     1621 aaacccgaaa gggcggctca ttgtcaagaa gaaagaatgg tccgtttgca caacgacccg
     1681 gaaataaaaa ttttttcttg acaatacgac acccgctgga gtattgggaa gcatattggt
     1741 tgaccgtcca atcaatattt ggatcatccg tccaatcctg gcacgccgct ggattgtcaa
     1801 aaccgcaccg tccggtgttt tgatggaatt tttatgggcg ccttgaatga aaataaaact
     1861 tacaaaatac agttcttggg agggcaacta tggatttctg gctgaaagac gagcagaaaa
     1921 tgcttcgcta caacattcga gaattcgccg aacaggaaat cgccccggtc gcaatggagt
     1981 tggatgaaaa ggaagaattt tcctgtgacc tgacccggaa aatggccaag cttggtctgt
     2041 tcggcattta tgtgcctgct gaattcggcg gagcgggcat ggattatctt tcctatatca
     2101 tcgcggttga ggaaatctgc cgaatcgacg gttcgcacgg cgcgacggtt gccgcgggca
     2161 actccctggg catcggcccg atttattatt acggaagcaa aaagcagaaa aatgagtggc
     2221 tgccgcgcct ctgcgccggc gaaatcctgg cgtccttcgg gctcaccgaa cccaatgccg
     2281 gctctgatgc cggcgccagc aagaccaacg cgcggctgga gaacgggcag tgggtggtga
     2341 acggcagcaa aattttcatc accaattcca ccagcaaaat ggccggcatc tgcaccgtgc
     2401 aggccattac cgggacaaaa ggccagaaag tcggccccta cggctcgatt gaaaccaagg
     2461 aagtgtcctg cattctcgtt ccgaacggaa cgccgggctt caccacgaag aagatgacca
     2521 agaaaatgat gtggcgctcc tccgataccg gcgagcttta tttcgacgac tgccgggtgc
     2581 cggaggaaaa tctgctggga aagcgcggcg aa
//
LOCUS       DAOSIM010000274         2609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_34269_length_2609_cov_7.933046, whole genome shotgun sequence.
ACCESSION   DAOSIM010000274 DAOSIM010000000
VERSION     DAOSIM010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34269_length_2609_cov_7.933046"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..485)
                     /locus_tag="PLR47_09780"
     CDS             complement(84..485)
                     /locus_tag="PLR47_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13280.1"
                     /translation="MMDALFSFFTAHPTVFTLLTIFIVIAILYFILSKFIKLAVLLLI
                     VILLAGGANLLSDPASMPGKIKQNAQTFITGGKQIWHTVSGLWKDASDIADRVQKAPK
                     EIKSSLKTAPEKTGERGEKKKSAPEKKPAAK"
     gene            603..1919
                     /gene="mtaB"
                     /locus_tag="PLR47_09785"
     CDS             603..1919
                     /gene="mtaB"
                     /locus_tag="PLR47_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOG13281.1"
                     /translation="MNMLKVGLATLGCKVNQCDSAALAESLKSAGFSITPFNAFADVY
                     IINTCTVTAFADFQARQLIRRALRQNPAARVVVTGCYAQTEPRTLAGIEGVSHVVGND
                     HKHDIPELLFRPVSGTPQILAGDILKQRCFPSAPACGLAGRTRAFFKIQDGCSAFCSY
                     CIVPFARGPSRSLPLGQVLDGARNFIARGFREIVLTGIHLGDYGHDLQPRTSLVRALN
                     ALLSNHPETRLRLSSIEPNEISNELLGLFKRFPNLCPHLHIPLQSGDDGILKSMKRRY
                     DKAFYRALIENVIRTAEDIAVGIDVMVGFPGEGEKEFCSTMELLEKIPAAYLHVFPYS
                     ERPGTAAAKLSPKVPATVKKARAAALRELGANKREAFARRFPGKVLPVLVEQTRDKTT
                     GLARGFSHNYLPVLLDKSTASLVNKIVNVRIDQIRDGKLIGRVLDE"
     gene            1912..>2609
                     /gene="rnc"
                     /locus_tag="PLR47_09790"
     CDS             1912..>2609
                     /gene="rnc"
                     /locus_tag="PLR47_09790"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOG13282.1"
                     /translation="MNNDRMQKLAELQEKLSYVFRDATLLSTALTHRSYVNENPQSGG
                     EDNERLEFLGDAVLGLCVSDLLMKKHPDFDEGTLSKIRSALVNEKPLADLALQLGLGD
                     CLLLGRGEEHSGGRAKESLLANALEAVIAAIYLDSSFSKTKTLLRRLMKPMMKDEAIA
                     AQSPDYKTALQELCQKKYKTAPIYTLLAESGPEHEKSFEMEVVIGDDIRKIGCGRSKK
                     EAQKQAAQKAWESL"
BASE COUNT          600 a          755 c          705 g          549 t
ORIGIN      
        1 cacgcccggg gacgaagcgg gcttgaggcg catcggcgtc aatctgacca gcgatccgaa
       61 tttttcaacc aacgatcttt ttatcactta gccgccggtt ttttctccgg agcgctcttt
      121 ttcttttcac cgcgctcgcc ggttttttcc ggcgccgttt tcagcgatga tttgatttcc
      181 ttcggggctt tctgcacccg gtccgcgatg tcgcttgcat ccttccagag gccgctgacc
      241 gtatgccata tctgttttcc gccggtgatg aaggtctggg cattttgctt gattttaccg
      301 ggcatggatg ctgggtccga aagcaggttt gcgccgccgg ccagcagaat cacaatgagc
      361 agaagaacgg ctaattttat gaatttgctc aggataaagt aaagaatggc gataacgata
      421 aaaatcgtca gcaacgtaaa aaccgtcgga tgcgccgtaa aaaaggaaaa caaagcgtcc
      481 atcattcagt ccccttcccg tcaggtgagc ctttagctca ttttgctcat aaaatccatt
      541 tgtttgtctt gacaagaaac cggttgtccc ctagaaaatc gctgtccaga aggatcaagg
      601 aaatgaatat gctcaaagtc ggcctcgcca cactggggtg caaagtcaat caatgcgatt
      661 ccgcggcgct ggcggaaagc ctgaaatccg ccggtttttc cataacgccg tttaacgcct
      721 ttgccgacgt ctatatcatc aacacctgca ccgtcaccgc atttgccgac tttcaggcgc
      781 gccagctcat ccgcagggcg cttcggcaaa atcctgcagc ccgcgtcgtc gtaaccggct
      841 gttacgctca gacggaaccc cgaacgctgg ccggcatcga aggcgtttcc cacgtggtag
      901 gcaacgacca taagcatgac attcccgaac tgcttttccg tccggtctcc ggcacgccgc
      961 aaattctggc gggcgacatc ctgaagcagc gctgcttccc ttccgccccc gcgtgcgggc
     1021 tggcgggacg gacccgcgcc ttcttcaaga ttcaggacgg atgcagcgcc ttttgcagct
     1081 actgcatcgt gccgtttgcc cgcggcccga gccgcagcct gccgcttggc caggtgctgg
     1141 acggcgcgcg gaatttcatc gcccgtggct tccgggagat tgttctcacc ggcattcatc
     1201 tgggagacta cggtcacgac ctgcagcccc ggaccagcct cgttcgcgcg ctgaatgcct
     1261 tgctgtcgaa ccaccccgaa acaaggcttc ggctcagctc catcgagccc aacgagatat
     1321 cgaatgaact gcttggactg ttcaaacgct ttccaaacct ttgcccgcat ctgcacattc
     1381 ccctgcaaag cggcgacgac ggcattttga aaagcatgaa acgacgctac gacaaggcct
     1441 tttatcgcgc gctcattgaa aatgtgatcc ggacggcgga ggatattgcc gtcggcattg
     1501 atgtcatggt cggcttcccc ggcgaagggg aaaaggaatt ttgcagcacg atggagctcc
     1561 tggaaaagat tcccgccgca tatctgcacg tcttccccta ttcggagcgg ccgggaaccg
     1621 ccgccgcaaa actgtctccc aaggttccgg caacggtcaa aaaggcgagg gcggccgccc
     1681 tgcgcgagct gggcgccaac aagagagagg ccttcgcgcg gcgctttccg ggaaaggtcc
     1741 ttcccgttct ggtggaacaa acccgggaca aaactaccgg actggccagg ggtttttcac
     1801 acaattatct tccggtactg ctggacaaat ccacagcttc tttagtaaat aagatcgtca
     1861 acgtcagaat cgatcagatt cgtgacggca agctgattgg aagggttttg gatgaataac
     1921 gacagaatgc aaaaactggc cgagcttcag gaaaagcttt cctatgtctt ccgcgatgca
     1981 acgctgcttt ccacggcact gacgcaccgc tcctacgtca acgaaaatcc ccagtcaggc
     2041 ggcgaggaca atgagaggct tgaatttctg ggcgacgcgg tcctggggct ttgcgtgagc
     2101 gatcttctga tgaagaaaca ccctgatttc gacgaaggca ccctgtccaa aatccgctcc
     2161 gcgctggtca atgaaaaacc cctggcggat ctggcgttgc agctgggtct gggagactgt
     2221 cttcttctgg gccggggaga agagcactcc ggaggccgcg ccaaggagtc ccttctggcc
     2281 aacgcgctgg aggcggtcat tgccgcgatc tatctggatt caagcttcag caaaaccaag
     2341 acgctgctca ggcgcctgat gaagcccatg atgaaggacg aggcgatcgc cgcgcaaagc
     2401 ccggattaca aaaccgccct gcaggagctt tgccagaaaa agtacaaaac cgctccgatc
     2461 tataccctgc ttgctgaaag cggccccgag cacgaaaaaa gctttgaaat ggaagtcgtc
     2521 atcggagacg acattcggaa gatcggctgc ggaagaagca aaaaagaagc gcaaaagcag
     2581 gcggcgcaaa aagcctggga aagtctgca
//
LOCUS       DAOSIM010000275         2607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_34310_length_2607_cov_7.641066, whole genome shotgun sequence.
ACCESSION   DAOSIM010000275 DAOSIM010000000
VERSION     DAOSIM010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34310_length_2607_cov_7.641066"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1465)
                     /locus_tag="PLR47_09795"
     CDS             complement(<1..1465)
                     /locus_tag="PLR47_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOG13283.1"
                     /translation="MNTDRSTNFFARQARARKNGRRQLIAFFFAVAVIVLVTTLAIRL
                     AWYLYVGTNAYTSFNAGEAHDYHQRLSTFTFFDPAFFIFMSMAIVIVILAASIYKMHT
                     LKKGGGAVAEMLGASRIDSKTTDPAKRRLINVVEEMAIASGIPVPQVFVLDSEPNINA
                     FAAGLELSDAAVTVTRGALTRLNRDELQGVVAHEFSHILNGDMRLNVQLIGILFGILF
                     LGIAGQKFLSGGRVSLRAGLPAMAAGIFLVIIGQAGSFLGRLMQCAIARQQEFLADAS
                     AVQFTRNPPGLAGALKKIGGGAFGSRIGSAKARQASHLFFSESHPASWFSFLDTHPPL
                     ITRIRLLDPSFDGKFPRMADEPIAPKPIYTDPFGGTGHYRRPSVRGASALAADVTSLV
                     GSPLPGHLEESRTILDALPQDVREMAATPRGAASVVFALLIGRDASRREQQAAALNRT
                     SVLQGDTEMVFSLGDQLAGLSNSLKLPLLELAMPSLSV"
     gene            complement(1474..2067)
                     /locus_tag="PLR47_09800"
     CDS             complement(1474..2067)
                     /locus_tag="PLR47_09800"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOG13284.1"
                     /translation="MIPLIVLVVIIAAAAFYGVGIYNHLITLRNLYRNAFTQIDIQLK
                     RRYDLIPNLVEAAKGYLAHERGTLEAVMKARNSAVDASKAAAQSPGSAAAMTGLSQAE
                     GALAGVLSRLLAVVEAYPDLKANQTIADLMEELTSTENKIGFARQAYNDAVTGYNIQR
                     EKFPQNVIASAFDFKAAALLDAVEKPEERTAPKVSFS"
     gene            complement(2130..>2607)
                     /locus_tag="PLR47_09805"
     CDS             complement(2130..>2607)
                     /locus_tag="PLR47_09805"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416620.1"
                     /note="catalyzes the formation of glutamate 5-phosphate
                     from glutamate in proline biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOG13285.1"
                     /translation="AAAKKVASIGIPCIIAPGKKKKVLSDIMAGREIGTLFLPQTAKL
                     ASRKYWIAFTLRPRGRLILDDGAKKALLEKGKSLLPSGITGVEGEFDPGDPVACVDRD
                     GNLLAKGLVNYSDGDVRRILGLKTAQIFSVLGHKDYDEVIHRDNLVVTGDLKKNKG"
BASE COUNT          488 a          745 c          849 g          525 t
ORIGIN      
        1 tgaccgacaa agacggcatg gccagttcca gaagaggcag cttcaggctg ttggacaacc
       61 cggccaactg atcgcccaga gagaaaacca tttccgtgtc tccctgcagg accgatgttc
      121 ggttcagcgc agcggcctgc tgttcacggc gtgaggcatc cctgccgatg aggagcgcga
      181 aaacgacgga ggctgcgccc ctgggtgtcg cggccatctc gcggacgtcc tgcggcaggg
      241 cgtccaaaat ggtccgactt tcttcaagat ggccgggcaa agggctgccg accagagagg
      301 taacgtccgc cgccagtgcc gatgcacctc gaacggaggg ccggcgataa tgcccggttc
      361 ccccgaacgg gtccgtgtag atgggtttgg gggcgatcgg ttcgtcggcc attcggggga
      421 acttgccgtc aaaggaagga tccagcaggc ggatgcgggt gatgagcggc ggatgcgtgt
      481 ccagaaagga aaaccagctt gcgggatggc tttcgctgaa aaataaatgg ctggcctgcc
      541 gcgccttggc cgatccgatg cgcgaaccga aggcgccgcc gccgattttt ttgagcgcgc
      601 ccgccaaccc cggcggattg cgcgtgaact gcaccgcgga ggcgtccgcc agaaattcct
      661 gctgacgggc gatggcgcat tgcatcaggc ggcctagaaa cgagccggcc tgcccgatga
      721 tgaccaggaa aatcccggcg gccatcgccg gaagccccgc ccgcaacgat actcttccgc
      781 cggagagaaa tttttgtccg gcaatgccca ggaaaagaat gccgaagagg atgccgatga
      841 gctggacgtt gaggcgcatg tcgccgttga gaatgtggct gaactcgtgg gcgacgacgc
      901 cctgcagttc gtcgcggttg agcctggtga gcgccccgcg cgtgacggtt accgccgcgt
      961 cgctcaattc cagtccggcg gcaaaggcgt tgatgttggg ttcggaatcc aggacaaaga
     1021 cctgcgggac tgggatgccc gaggcgatgg ccatttcctc caccacgttg atcagacggc
     1081 gtttggccgg atccgttgtt ttggagtcga tccggctggc gcccagcatt tccgccacgg
     1141 cgccgccgcc ttttttcaga gtgtgcatct tgtagatgct ggcggccagg atcacgatga
     1201 caatggccat ggacatgaag atgaaaaacg cgggatcgaa aaacgtgaag gtggaaagcc
     1261 tctggtggta gtcgtgggct tcgccggcgt tgaaggaggt gtaggcgttt gtgccgacat
     1321 acaggtacca ggccaggcgg atggccaggg tcgtcaccag cacaattacg gccacggcga
     1381 agaaaaacgc gatgagctgc cgccggccgt ttttgcgggc cctggcctgc ctggcaaaga
     1441 agttggtcga acggtcggtg ttcatgcttt ccgtcaggag aaagacactt tgggcgccgt
     1501 ccgctcttcg ggtttttcca cggcgtccag aagggcggcg gccttgaaat caaacgcgga
     1561 cgcgatcacg ttctggggaa atttttcccg ctgaatgttg tagccggtga cggcgtcgtt
     1621 gtaagcctgc cgcgcgaagc cgatcttgtt ttccgtcgag gtcagctcct ccatcagatc
     1681 ggcgatggtc tggttggctt tcagatccgg gtaggcttca accaccgcca gcagcctgga
     1741 aagaactccg gcaagcgccc cttccgcctg gctcaagccc gtcatggccg cggcgctgcc
     1801 gggagactgg gcggccgctt tgcttgcgtc cacggcggaa tttcgcgctt tcatgacggc
     1861 ttcgagcgtg ccgcgttcgt gcgccaggta gcctttggcc gcctccacca ggttgggaat
     1921 cagatcgtag cggcgcttga gctgaatgtc gatctgggtg aaggcgttcc ggtaaagatt
     1981 gcgcagcgtg atcaggtgat tgtaaattcc gacaccataa aacgcggcgg cggcgatgat
     2041 gacgaccaga acgatcaatg gaatcatgtt aacctcctta acgttgcgcc cggacgggcg
     2101 catttctggc gctgatcgga tttatcggat catcctttgt ttttcttgag gtcgccggtg
     2161 acgaccagat tgtcgcggtg gatcacctcg tcatagtcct tgtggcccag cacggaaaaa
     2221 atctgtgcgg tcttcagtcc cagtatccgg cggacatcgc cgtcgctgta attgacgagc
     2281 cccttggcca gcaggtttcc atcgcggtcc acgcaggcga cgggatcccc cggatcgaat
     2341 tccccttcca cgccggtgat gcccgacggc agcaggcttt tgcctttttc gagcagggct
     2401 ttcttggcgc cgtcgtcgag aatcaggcgt ccgcgggggc gcagcgtgaa ggcgatccag
     2461 tacttgcggc tggccagttt cgcggtctgc ggcagaaaca gcgtgccgat ttcccggccc
     2521 gccatgatgt cggacagcac tttctttttc tttcccggcg cgatgatgca gggaatgccg
     2581 atggaggcga cttttttggc ggcggcg
//
LOCUS       DAOSIM010000276         2605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_34353_length_2605_cov_7.041961, whole genome shotgun sequence.
ACCESSION   DAOSIM010000276 DAOSIM010000000
VERSION     DAOSIM010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34353_length_2605_cov_7.041961"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /gene="rpsK"
                     /locus_tag="PLR47_09810"
     CDS             complement(<1..270)
                     /gene="rpsK"
                     /locus_tag="PLR47_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOG13286.1"
                     /translation="MAKAVRKTGKKKEKKNITEGVAHIQSTFNNTIITITDMTGNVIS
                     WSSAGLQGFKGSRKSTPFAAQMAAEDAVRKAKDQGMRRVQVYIKGP"
     gene            complement(298..>634)
                     /gene="rpsM"
                     /locus_tag="PLR47_09815"
     CDS             complement(298..>634)
                     /gene="rpsM"
                     /locus_tag="PLR47_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOG13287.1"
                     /translation="NKRMEVALTYIYGIGKTRAMQILKESGVSPDTKTDQLADSEISA
                     IRGIIDKEGKVEGDLRREVSMSIKRLMDIGAYRGLRHRKGLPVRGQRTHTNGRTRKGP
                     RRAIAGKKK"
     assembly_gap    635..644
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<645..746)
                     /gene="rpmJ"
                     /locus_tag="PLR47_09820"
     CDS             complement(<645..746)
                     /gene="rpmJ"
                     /locus_tag="PLR47_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOG13288.1"
                     /translation="MKVRSSVKKICEKCKIVKRKGVLRVICENPKHKQ"
     gene            complement(781..999)
                     /gene="infA"
                     /locus_tag="PLR47_09825"
     CDS             complement(781..999)
                     /gene="infA"
                     /locus_tag="PLR47_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOG13289.1"
                     /translation="MAKEEAIEVEGRVLEPLPNAMFRVELENGHKVLAHISGKMRMHF
                     IKILPGDKVTVELSPYDLTRGRITYRTK"
     gene            complement(1036..1785)
                     /gene="map"
                     /locus_tag="PLR47_09830"
     CDS             complement(1036..1785)
                     /gene="map"
                     /locus_tag="PLR47_09830"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOG13290.1"
                     /translation="MVILKQPDEIDKARASSRIVAEVLSVLREKVKPGVTTRELDQIA
                     EGVTEKRGARPAFKGYRGYPHSLCASVNEEVVHGMPSGRILKEGDIIGLDFGVLYHGF
                     YGDAAITLPVGRVSEEAVRLMRVTEESLCAAIDQAASGNRLGDISAAVQKTAESAGFS
                     VVRDFVGHGIGRNMHEDPQIPNFGKKGRGIELRTGMILAIEPMVNAGRYGVKILPDGW
                     TVITADGSLSAHFEHSVAITDNGPEILSSLN"
     gene            complement(1810..>2605)
                     /locus_tag="PLR47_09835"
     CDS             complement(1810..>2605)
                     /locus_tag="PLR47_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HOG13291.1"
                     /translation="GETITEKGIGNGISLIIFAGIVCNLPAAIGNTWRLYSSGELHFL
                     ILVLLGLFMIAVIGAIVYVEAAQRRIPVQYAKRIVGRKMYGGQTTHLPLKINSAGVIP
                     PIFASSVIMFPATIANFAPAGLMKTISGYLMPGRLGYEILFVGLIFFFCFFYTAVTIK
                     PDDMAENMKKFGGYVPGIRPGKKTAEYIEEILERLTFSGAIYVSIICVLPSVLISQLN
                     TPFYFGGTSLLIVVGVAMDTASQIESHLLTRQYEGFMKKGHMARRR"
BASE COUNT          530 a          806 c          628 g          631 t
ORIGIN      
        1 cggacctttg atataaacct ggacgcggcg catgccctga tccttggctt tgcgaaccgc
       61 gtcttccgcc gccatctgcg cggcaaacgg cgtgctcttg cgcgaaccct tgaagccctg
      121 cagccccgcc gaggaccagg agatcacgtt gccggtcatg tccgtaatcg tgatgatcgt
      181 attattgaaa gtggattgaa tatgggcaac cccttccgta atatttttct tttctttttt
      241 ctttcccgtt tttctgactg ctttcgccat ggttcctccg gtcgaaaatg tgttgcgcta
      301 ttttttcttg cccgcgatcg cccgtctcgg gccttttctc gtccgtccgt tggtgtgcgt
      361 tctctgcccc cggaccggaa gccccttgcg gtggcgcagg ccgcggtaag cgccgatatc
      421 catcagtcgc ttgatcgaca tggaaacctc acgccgcaga tccccttcga cctttccctc
      481 tttatcgata atgccgcgaa tggccgatat ctcggagtcg gccagctggt cggtcttggt
      541 gtccggactg acgccagatt ccttcagaat ctgcatcgcc cgggttttgc cgatgccgta
      601 aatgtacgtt aaggcaactt ccattcgctt gtttnnnnnn nnnnctgctt atgtttgggg
      661 ttttcacaaa tcacgcgcaa aacacccttg cgtttgacga ttttgcactt ctcgcatatt
      721 tttttcactg acgatcgtac tttcacgact tccactcctt ccggccggca accaaggctt
      781 tcacttcgtc ctgtacgtga tccggccccg ggtcaaatca tagggggaaa gctccaccgt
      841 aaccttgtcc ccgggcaata tttttataaa atgcatccgc attttgccgg atatgtgcgc
      901 cagaacctta tgtccgtttt ccagttccac tcgaaacatg gcgttcggca ggggctccag
      961 gacccgacct tcaacttcta ttgcttcctc tttggccata cattactcaa gtaccggttc
     1021 atgttcatga aaaaatcagt tcaaactgct caatatctcc ggcccgttgt ccgtgatcgc
     1081 aaccgaatgc tcgaaatggg ccgacaaact tccgtccgcc gtaatcaccg tccagccgtc
     1141 cggtaaaatc ttgaccccgt acctgcccgc gttgaccatc ggctcgatgg cgagaatcat
     1201 ccccgtccgc agctcgattc cccggccttt ttttccgaaa ttcggaatct gggggtcctc
     1261 gtgcatgttc ctgccgatgc cgtgtccgac aaaatcccga accacggaaa atccggccga
     1321 ttccgccgtt ttctgaaccg ccgccgaaat atcccccagg cggttcccgg aagcggcctg
     1381 gtcaattgcc gcgcagaggc tttcttcggt cacgcgcatc agccgaaccg cctcttcgct
     1441 cacccggccg acgggaagcg tgatcgccgc atccccgtaa aagccgtgat agagaacgcc
     1501 gaagtccagt ccgataatgt cgccttcttt caagatccgg ccggacggca ttccgtgaac
     1561 gacttcctcg ttgaccgacg cgcacaggga atggggatag ccccgataac ctttaaacgc
     1621 cggtctggcc ccacgctttt ccgtgacccc ttcagcgatt tgatccagct cccgcgtggt
     1681 cacgcccggt tttacttttt cccgaaggac gctcaggact tccgccacaa tccggctgct
     1741 cgctctggct ttgtcgatct catccggctg tttcagaatc accatgagcc ccaaccatcc
     1801 atccgcaatc tatcgtctgc gcgccatatg tccttttttc atgaacccct catactggcg
     1861 ggtcagcaga tgcgattcga tctggctggc cgtgtccatg gccacgccga caacaatcag
     1921 cagagacgtg ccgccgaaat agaaaggcgt gttcaactga gaaatcagca cactgggcag
     1981 cacgcagatg atggaaacat aaatcgcgcc gctgaaggtg agtctctcca gaatttcctc
     2041 gatatattcg gcggttttct tgcccgggcg gattcccgga acataccctc cgaatttctt
     2101 catgttttcc gccatgtcat ccggtttgat cgtcacggcc gtatagaaaa agcaaaagaa
     2161 gaaaatcaat ccgacaaaaa gaatttcata gcccagccgc ccgggcatca ggtagcccga
     2221 aatcgtcttc atcaggccgg ccggcgcaaa attggcgatg gtcgccggaa acataatcac
     2281 cgacgacgcg aaaatcggcg gaatgacgcc cgcggaattg atcttgagcg gcagatgagt
     2341 cgtctgtccg ccgtacattt tccgtcccac gattcttttg gcgtactgga ccggaattcg
     2401 tctctgcgcg gcttccacgt aaacaatcgc gccgatcacg gcaatcatga acagccccag
     2461 cagcaccagg atgaggaaat gcagttcgcc ggacgaatac aggcgccagg tgttgccgat
     2521 ggccgccggc agattgcaga caatgcccgc gaaaatgatc agcgagatgc cgttgccgat
     2581 ccccttctcc gtaatggtct ctccc
//
LOCUS       DAOSIM010000277         2574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_34867_length_2574_cov_6.718142, whole genome shotgun sequence.
ACCESSION   DAOSIM010000277 DAOSIM010000000
VERSION     DAOSIM010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34867_length_2574_cov_6.718142"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PLR47_09840"
     CDS             <1..292
                     /locus_tag="PLR47_09840"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG13292.1"
                     /translation="RIDAESCTGCGVCVKRCQMDAIVADGKIFRVDPDRCIGCGLCAT
                     RCKPKAARLIRKDRITVPPMNTEMLYLSILMERAGRKKMIVNMLKLLFGQPL"
     gene            complement(321..1367)
                     /locus_tag="PLR47_09845"
     CDS             complement(321..1367)
                     /locus_tag="PLR47_09845"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13293.1"
                     /translation="MTTNLENLSATLVSVLTAFEAGMRRLHPLAVPAIRGELAVYEPV
                     LAAVRASLKTEDCNARSEEDRQALLRACDFLLQAVKNFGREEDLQDAFFSALRASRRT
                     CRAQEALFPLCRLFPAVHRYFSEPGADILPEPSTGDSRVGLMHVGANSDLRARGGYSL
                     YIPSSPFLASARPLVVALHGGYGHGRDFIWTWIREARSRGFVICAPTAQAMTWSIGRI
                     DLDGKALTQHLEEVCSRVPIDRNRILLTGMSDGGTFALALGLTREEGIFSKIAAVSCA
                     LPPVKINRPEEKHVLWIHGAQDWIFPVERTVQASRYLQQSGVDIKLKVIPDLSHAYPR
                     ETNGTILKWFGIEG"
     gene            complement(1364..>2574)
                     /locus_tag="PLR47_09850"
     CDS             complement(1364..>2574)
                     /locus_tag="PLR47_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG13294.1"
                     /translation="EESEIVGGRARSFRDEEGYIREYGAHSHRLAHKGIANEVFRRLG
                     EKIDFLPEAHDGKLIFKGRLWERPEGPIGFLKTPMLSFRARLVLLALFAKIKKTDPAT
                     WYHKTLLDFYKTSFRNPEVEAFLPFLGMTIMCPDPDKVSAGEVIDFLKRVLAAGVGVG
                     EPRGGSAGIFQTLSRHIDRGGEIHLHEKAEKIIIESRRVTGVKTGRATYPASVVLFAA
                     RLPLAVDLLDRSLLPATVVNYAKNIEHSSGLVIDFITDRPITGIRAGILGVDVPIWAR
                     FQSSADPSFTPDGKHLSSWGIMLPWHFDGDASVVAGAEQRMKSVISAVFPDFLPAVIE
                     ERKIVATVMNGNVLTPAQSKPNRPDIECSGISGLYFIGDTVRGDGCSGDISFSSAMKA
                     ADKILSDQKV"
BASE COUNT          576 a          758 c          707 g          533 t
ORIGIN      
        1 gcgcatcgac gccgaatcct gcacgggatg cggcgtctgc gtgaagcgct gtcagatgga
       61 cgcgatcgtc gcggacggca aaatcttccg ggtggatccg gaccggtgca tcggctgcgg
      121 cttgtgcgca acccggtgca aacccaaggc cgctcgtctc atcagaaaag acagaatcac
      181 cgtgcctccg atgaacacgg aaatgctgta cttgagcatc ctgatggaac gggcgggaag
      241 aaaaaagatg atcgtcaaca tgctgaagct tcttttcggc caaccattat aaagggatct
      301 cccatgcctg ccttcgggca tcatccctca attccaaacc atttcaggat tgtgccgttt
      361 gtttcacgcg ggtaggcatg tgatagatcg ggaatcacct tgagcttgat atccacgccc
      421 gattgttgca aataccggct agcctgcacc gtccgctcca ccggaaagat ccaatcctgc
      481 gcaccgtgaa tccacagcac atgtttttct tcgggccggt ttatcttcac cggaggcaaa
      541 gcgcaggaaa cggctgcaat cttcgagaaa ataccttcct cccgggtcag tcccagggcc
      601 agggcaaagg tcccgccgtc ggacattccg gtcaaaagaa tgcggtttcg gtcaatggga
      661 acgcgggagc aaacctcctc cagatgttgc gtcagggctt ttccatcgag atcaatgcgg
      721 ccgattgacc aggtcatggc ctgcgcggtt ggggcgcaaa taacaaaccc cctgcttctc
      781 gcctcccgaa tccaggtcca gataaaatct ctgccgtggc cgtatccgcc atgcagcgcg
      841 acaaccagcg gccgggcgct cgctaagaaa ggggatgaag gaatgtagag ggaataaccg
      901 cccctggcgc gcaggtcact gtttgcaccg acatgcatca ggccgacgcg ggaatccccg
      961 gtggatggct cgggcaagat gtcagctccc ggttccgaaa agtaacggtg aacggcgggg
     1021 aaaaggcggc agagtggaaa cagcgcttcc tgcgcccggc atgttctgcg tgaagcgcga
     1081 agcgcggaga aaaaagcgtc ctgaaggtct tcttccctgc cgaaattttt gacggcctga
     1141 aggagaaaat cgcaggcgcg caaaagcgcc tgacggtctt cttccgaccg ggcgttgcag
     1201 tcttccgtct tcaatgatgc ccggacagcc gccagcacgg gttcataaac ggccagttca
     1261 ccccgaatgg cgggaaccgc cagggggtgc agtctgcgca tgcccgcttc gaatgccgtc
     1321 aaaacggaaa ccagcgtcgc cgataggttt tccaggtttg ttgtcatacc ttctgatcgc
     1381 tcaggatctt gtccgcggct ttcattgcgg aagaaaaact gatgtcgccc gagcagccgt
     1441 cccctctcac cgtatcgccg ataaaataga gtccactgat tcccgaacat tcgatatccg
     1501 gccggttcgg tttggactgc gccggcgtca ggacattccc gttcatgacc gtcgcgacaa
     1561 tttttctctc ttcgatcacc gcgggcaaaa aatccggaaa aacagccgag atcacgcttt
     1621 tcattctttg ctccgcaccg gcgacgacgc tcgcgtcgcc gtcgaaatgc cacggcagca
     1681 taattcccca actggaaaga tgcttcccgt cgggggtaaa cgaaggatcg gcgctggact
     1741 ggaagcgcgc ccagatcggg acgtctacgc cgagaatccc ggcacgaatg ccggtaatgg
     1801 gtctgtccgt gatgaaatca atgaccagac cggaactgtg ctcaatattt ttcgcgtaat
     1861 tcacaacggt cgcgggcagc agtgaacggt cgagcagatc aacggccagg ggaagccggg
     1921 cggcaaaaag cacaaccgac gctggatagg tcgcccgtcc ggttttcacg cccgtgaccc
     1981 gccggctttc gatgatgatt ttttccgctt tttcatgaag gtgaatttca ccgcccctgt
     2041 cgatgtgccg gctcagcgtt tgaaagattc cggcggatcc gccgcgaggc tccccgacgc
     2101 cgacaccggc cgccagcacc cgctttaaga agtcgatcac ttcacccgcg ctgaccttat
     2161 ccggatcggg acacatgatc gtcatcccca gaaacggcag gaaggcctcg acttccggat
     2221 tccggaagga ggttttgtaa aaatccagga gtgtcttatg ataccaagtc gccggatcgg
     2281 tctttttaat tttggcaaac aatgccagaa gcaccaacct ggcccgaaac gacagcatgg
     2341 gggttttcaa aaaaccaatc ggcccttcgg gtctttccca gagcctgcct ttgaagatca
     2401 gtttgccgtc atgtgcctcg ggcagaaaat cgattttttc tcccagtctt ctgaacacct
     2461 cgttggcgat gcccttgtgc gcaagcctgt gcgaatgcgc cccgtattcc cgaatatatc
     2521 cttcttcatc ccgaaacgat cgggcgcggc cgccgacgat ttcggattct tcca
//
LOCUS       DAOSIM010000278         2555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_35194_length_2555_cov_7.338400, whole genome shotgun sequence.
ACCESSION   DAOSIM010000278 DAOSIM010000000
VERSION     DAOSIM010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35194_length_2555_cov_7.338400"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="PLR47_09855"
     CDS             complement(<1..958)
                     /locus_tag="PLR47_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOG13295.1"
                     /translation="MAKYEPKQLRNLAVIGHGGTGKTSLCESLLFITGKNERLGRVDD
                     NTSTMDFEPEEQKKHVSISSAVNFVEWDKHKINIVDTPGDSNFTFDIQCSLSIVDNAV
                     VVIDSVGGVEFQTQKVWEIANGYSLPRLIFVNRMDRERADFNKVLESIKTKLKKKATP
                     VCLPIGAEDKFQGIVDLIAMKAYTFADNKGAGKAVDIPADLADEAAELRSSMVEDIAE
                     ADDELMNKYLEAGELSAEEIMSGLRKGVIAGTLLPVICGSAVKGIGVTLLADLAVSSF
                     ASPVDRGPVKGKKPQTDSVEERQPSEDAPFSAIVFKTIADPYA"
     gene            complement(1122..>2555)
                     /locus_tag="PLR47_09860"
     CDS             complement(1122..>2555)
                     /locus_tag="PLR47_09860"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13296.1"
                     /translation="INSLSHMYLSRDLSLLHSLPVDVRDAFLSRWLGSTFDSSWMVVA
                     FSLPVFLSYGLVYQAGLAFYPIALSAIVCLCLLASAASSLLVLLGAAILPAGKIRTLF
                     VIMGVGLALLLILVLRMIRPEQLVNPDSFASVVLYLNSMQALDWPWLPTTWITDTVRL
                     ALKREWGPSLFSLTLSASCFFSLMFFTQTAAGALYFRGFSRAQTTAKRLFTPVKFHGY
                     SWETLLNFLSRPARAFAVKEIRSFFRDSTQWPQLFLICALIVVYLYNFSVLPLDKSPM
                     KTVYLQNVFSFLNVGLAAFVLSAIAARFVFPAVSMEGEAFWIIQSAPVSMKTFLWIKF
                     FLYYIPLLMLSEILIAASNILLGVTPFMMFLSTVTIFCLVPAIVGLAVGLGARYPNFK
                     SENPALSATSFGGVLFMLLSFGLIALVIMLEAGPVYRIFMSRMHGHAFSLLQILWFIL
                     SFCLALFLCVLAVFYPMRQGEKNLLKR"
BASE COUNT          666 a          639 c          738 g          512 t
ORIGIN      
        1 ccgcgtaagg gtcggcgatg gttttgaata cgatggctga gaaaggagcg tcttccgagg
       61 gctgcctttc ttcgacgctg tccgtttgag gttttttgcc tttgaccggc ccgcggtcca
      121 cgggggatgc gaaagagctg acggccagat ccgcaagcag agtgacgccg atgcctttaa
      181 cggcggagcc gcaaatgacg ggaagaaggg ttcccgcaat tacgcctttt cgcaaaccgg
      241 acatgatctc ttcagcggaa agttcgccgg cttccaggta tttattcatc aattcgtcat
      301 cggcttcggc aatgtcttcc accatcgatg agcgaagctc ggctgcttca tcggccaggt
      361 cggctggaat atccacggct tttcccgcgc ctttgttgtc ggcgaaggtg taagccttca
      421 tggcgatcag gtccacgatg ccctgaaatt tgtcttccgc gccgatcggc agacagacgg
      481 gggttgcttt tttcttgagc tttgttttga tgctttccag cactttattg aaatccgccc
      541 gttcgcggtc catgcggttg acgaaaatca ggcgcgggag agaataccca ttcgcgattt
      601 cccagacttt ttgcgtctgg aattcgacgc cgcccacgga gtcaattacg acgacggcgt
      661 tgtccacgat gctcagggaa cattgaatat caaaggtaaa gttggagtca cccggcgtat
      721 ccacgatatt gattttatgc ttatcccatt cgacaaaatt gaccgccgat gagatggaaa
      781 catgcttttt ctgctcttcc ggctcaaagt ccatggtgga cgtattgtcg tcaacgcgtc
      841 ccagacgttc atttttgccg gtaataaata aaagggattc acaaagagag gttttgcctg
      901 tgccgccgtg gccgataacg gccagatttc tgagttgttt gggttcgtac tttgccatgg
      961 aaactccttt cgatatctca acaaatccgc agaagatctg atgaaacggg tcattgcaga
     1021 atgcgcgttt gtttagcgta tccaacaagt aaaatcaaga taattttcgg cttctggcga
     1081 ttttgaggct cctttttccc ccggggaagc cggacggagg atcatctctt cagcaggttc
     1141 ttttcgccct gcctcatggg gtagaaaacc gcgaggacgc aaagaaacag cgcaagacaa
     1201 aaggacagga taaaccagag gatctgaagc agggaaaagg catggccgtg cattctggac
     1261 atgaagattc tgtaaaccgg accggcctcc agcatgatca ccagggcgat cagaccgaaa
     1321 gaaagcagca tgaaaaggac gccgccgaaa ctggtggccg acaaggccgg attttccgac
     1381 ttgaagttcg ggtagcgggc gcctaagcca acggccagcc cgacgatggc cgggaccagg
     1441 cagaagatgg tgacggtgga caaaaacatc atgaacggag ttacgcccag cagaatgttg
     1501 gatgcggcaa tcagaatttc ggacagcatc aaaagcggaa tatagtagag aaaaaacttg
     1561 atccacagga atgttttcat cgaaacgggg gcggattgaa tgatccagaa cgcttctcct
     1621 tccatgctca ccgcgggaaa aacaaaacgg gcggcaatgg cggaaagcac gaaggcggcc
     1681 agccccacgt tcaaaaatga aaacacattc tgcaaataga ccgtcttcat gggcgattta
     1741 tccaggggca gaacggagaa gttgtagaga tagacgacga tcagggcgca gatcagaaag
     1801 agctgcggcc actgcgtcga atcccggaaa aaggagcgga tttcctttac ggcaaaggct
     1861 ctcgccggcc gggaaagaaa attcagaagc gtttcccaac tgtatccgtg gaatttcacg
     1921 ggggtaaaaa gccgtttggc ggtggtctgg gcgcgggaaa acccccgaaa atagagggcg
     1981 cccgcggcgg tttgggtaaa aaacatcaat gaaaagaagc agcttgccga gagcgtcagg
     2041 ctgaaaagcg acggacccca ttcccttttc agggccagac gaaccgtatc cgtgatccag
     2101 gtggtgggaa gccacggcca gtccagcgcc tgcatggagt tcaggtacag aacaaccgag
     2161 gcgaagctgt ccggattgac cagctgctcc ggacgaatca tgcgcagaac caaaataagg
     2221 agcagcgcca ggcctacgcc catgatgacg aaaagagtcc ggatctttcc ggcgggaaga
     2281 atggccgcgc caagaagcac cagcaggctg cttgccgcgg aagccagcag gcacagacag
     2341 acgattgcgg aaagcgcgat ggggtaaaag gcaaggccgg cctgatagac gaggccgtag
     2401 gacaggaaaa cgggaaggct gaaggccacc accatccagg aagaatcgaa cgtgctgccc
     2461 agccagcgcg acaggaatgc atccctgacg tcgaccggca gggaatgcag cagggacagg
     2521 tcccggctta aatacatgtg cgacaggctg ttgat
//
LOCUS       DAOSIM010000279         2545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_35355_length_2545_cov_5.995582, whole genome shotgun sequence.
ACCESSION   DAOSIM010000279 DAOSIM010000000
VERSION     DAOSIM010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35355_length_2545_cov_5.995582"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..792)
                     /locus_tag="PLR47_09865"
     CDS             complement(19..792)
                     /locus_tag="PLR47_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG13297.1"
                     /translation="MAHFKVEHLSISFGGIKAVNDVSFEVEHNKIFSIIGPNGSGKTT
                     IFNMISGIYKPGAGEVLLEGENLVGLRPDTIARRGVARTFQNIQLFGNATVMDNLMLG
                     RHIHMKTGILSGAFMWGRHSPSAKEEIRHRELIEHIVDFLDLQSLRDQFVSNLPYGKR
                     KLVELGRALALEPKVLLLDEPSAGMNTEEKDDLRIWIKDIQEDYQVTILLIEHDMNMV
                     MGISDRILAISQGVKIIEGTPQEVQKHPDVIAAYLGEED"
     gene            complement(876..2063)
                     /locus_tag="PLR47_09870"
     CDS             complement(876..2063)
                     /locus_tag="PLR47_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG13298.1"
                     /translation="MKKVLMAVMILCAVLMIPFVAGAGKPGFDDKEIRIAQWGPQTGP
                     AAPWGSVARGSDILFKIINDEGGIHGRKIKYFIRDDQYNPAQTKAVVKELVEREGIFA
                     FVGGVSAAAGMAVKGYLAENKVIWVGPATAINEYVFPVQPYLFGIYPLYDDEASILTK
                     YAAQKLKAKKIAFFYQNDAYGKSGLDGCKDRMKALKMQLVQEVSTEPGEKDLSSQILR
                     LKNSGADVVIMWVNPTSAVIALKTAATVGFKPQWISSNTLSDYVLMNKITGGLWEGVI
                     TGAFAEAPDSNTPLLKKYREAAKKYAPEERWGTFYMAGILFAEPLVEAIKRAGRNLST
                     DAVLKQLNSMKKFQGIGPPVTWTPKVHQGSDAIMIQQCGPNSSYITLQNWTANELATW
                     KKK"
     gene            complement(2127..>2545)
                     /locus_tag="PLR47_09875"
     CDS             complement(2127..>2545)
                     /locus_tag="PLR47_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13299.1"
                     /translation="CAAVLIAGLLIWDAPRNRVQLFWEKGAIEEAAHRYFQAEKAGDL
                     KQTYALLAPSSAYRQAHSYDSYLKDIAGYPPVKIDTYQIVDVYRLRDNDRRDQYPGVD
                     KLVQVEVEMTFVNSGRNNVFNYSFTFLKEKGSWYKG"
BASE COUNT          485 a          763 c          683 g          614 t
ORIGIN      
        1 tatttttgac cgagagcatt agtcctcctc tcccagatag gcggcaatca cgtcggggtg
       61 cttctgcact tcctgcggcg tgccctcgat aatcttcacg ccctgactga tggccagaat
      121 cctgtcggag atgcccatca ccatgttcat gtcgtgctcg atgagcagaa tcgtcacctg
      181 atagtcttcc tgaatgtcct tgatccagat gcgcaaatcg tccttttctt cggtgttcat
      241 gccggcggac ggttcgtcca gcaggagcac cttgggctca agggccagcg cccgccccag
      301 ctccaccagt ttgcgcttgc cgtagggcag gttggacaca aactggtcgc gcagggactg
      361 caggtccaga aaatcgacga tgtgctcgat gagttcgcga tgccggattt cctccttcgc
      421 gctcggagaa tgccgccccc acatgaaagc gccgctgaga atccccgttt tcatgtggat
      481 gtgccgcccc agcatcaggt tgtccatgac cgtggcgttg ccgaaaagct gaatgttctg
      541 aaacgtccgc gccacgcctc tgcgggcgat cgtgtccgga cgcaggccga ccagattctc
      601 gccttccagc agcacctctc cggcgccggg cttgtagatg ccgctgatca tgttgaagat
      661 cgtggtcttg ccgctgccgt tcggtccgat gatcgaaaag attttattgt gctccacttc
      721 gaagctgaca tcattgactg ccttgatgcc gccgaaagag atgctcaggt gttccacttt
      781 aaagtgcgcc ataagccttt taacccgtca ccgccaccac cggtggttat cgccgggaga
      841 aggcggttcg acccgccttc ccccggcgtc ttgaattatt tcttcttcca ggttgccaac
      901 tcgttggcgg tccagttctg cagcgtgatg taggaggaat tggggccgca ctgctgaatc
      961 ataatggcgt ccgatccctg atgcaccttc ggcgtccagg tcaccggggg gccgatcccc
     1021 tgaaactttt tcatcgagtt gagctgcttg agcaccgcgt ccgtgctgag gttgcggccg
     1081 gctcttttga tcgcttcgac cagcggttcg gcaaagagaa tgcccgccat gtagaaggtg
     1141 ccccagcgtt cttccggcgc gtatttcttg gcggcttccc ggtatttctt caaaagcggc
     1201 gtgttggaat cgggcgcctc ggcaaacgct ccggtgatca cgccttccca caatccaccg
     1261 gtgatcttgt tcatcagcac gtagtcggaa agcgtattgg aggatatcca ctgcggtttg
     1321 aagccgacgg tcgcggccgt cttgagcgcg atcacggccg aggtgggatt gacccacatg
     1381 atcacgacgt cggccccgga atttttcagg cgcaggatct gggaagacag gtctttttcg
     1441 cccggctccg tggaaacttc ctgcaccagc tgcatcttga gggctttcat ccggtccttg
     1501 catccatcca ggccgctttt gccgtaggcg tcattctggt agaagaaagc gatttttttg
     1561 gctttgagct tctgggccgc gtatttggta agaatgctgg cctcgtcgtc atacaggggg
     1621 taaatcccga aaagataggg ctgcacgggg aatacatatt cgttgatggc cgtggccgga
     1681 ccgacccaga tcactttgtt ctcggccaga taccccttga ccgccatgcc ggccgccgcg
     1741 ctcacgcctc cgacaaaggc aaaaatgcct tcgcgctcca ccagttcctt caccaccgcc
     1801 ttggtctggg cgggattgta ctgatcgtcg cggatgaagt atttgatttt gcggccgtga
     1861 atgccgcctt cgtcgttgat gattttaaac agaatgtcgc tcccccgggc aaccgacccc
     1921 cagggcgcgg cgggccctgt ctgaggcccc cactgagcaa tccggatttc cttgtcgtca
     1981 aatcccggtt tgcccgcgcc ggccacaaac ggaatcatca ggaccgcgca taaaatcatg
     2041 actgccatca gtaccttttt cataacccct ccttttgttt cttgtggaat gttgacggtg
     2101 ctttctcctt caaacccttt tgtgtttcat cccttgtacc agcttccctt ttccttgaga
     2161 aaagtgaaac tgtaattaaa aacattattc ctgcccgaat tgacaaaggt catttccact
     2221 tccacctgaa ccagcttgtc cactccggga tactgatccc ggcgatcgtt gtcccgcagg
     2281 cgatagacat cgacaatctg ataagtatcg atctttacgg gcgggtagcc ggcaatatcc
     2341 ttcaagtacg aatcgtagga gtgcgcctgc cggtaagcgg acgaaggcgc cagaagcgca
     2401 taggtctgct tcaggtcccc ggccttttcc gcctggaaat accggtgggc ggcctcttca
     2461 atcgccccct tttcccaaaa aagctgcacc cggttgcggg gagcatccca gatcagcagc
     2521 ccggcgatca gcaccgccgc gcagg
//
LOCUS       DAOSIM010000280         2531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_35624_length_2531_cov_6.234249, whole genome shotgun sequence.
ACCESSION   DAOSIM010000280 DAOSIM010000000
VERSION     DAOSIM010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35624_length_2531_cov_6.234249"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PLR47_09880"
     CDS             <1..610
                     /locus_tag="PLR47_09880"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG13300.1"
                     /translation="PGDARPVFLFLSHFHLDHIAGLHTLTKFLLGGGLTICCPAGGRD
                     VLQTFVNAPFTVPLEGLPYPVRMLELPGEAASLPFPVQALPLLHSGLTLGYRLEIGAK
                     VIAYCPDTGYCENAVILARGADLLITECAYKEGQSSESWPHLNPETAGRIAREARALR
                     LALTHFDARIYRSLPERMEAQTAARKEFPAAFAACDDMILEC"
     gene            620..2209
                     /locus_tag="PLR47_09885"
     CDS             620..2209
                     /locus_tag="PLR47_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOG13301.1"
                     /translation="MKFKLFPNTAPGKCLLASRHFPWAFVRRLPFLPAAVLFLTLLAS
                     CAPQYFSPVPGDAFTRKDLRELSGRELWHGFVFNGEKVGFARLKIEPDANGQAYTLFS
                     EASLRIRFLGMGKSISIRGEDRVAPDLSLISFQYEQTMDDKPLILKGEIRDGRLLVVQ
                     RSGSEAKTFETKISDPLYPASGINLYPVLQGMKTGDDYAYPVYDPQTQSVVDVSQSVV
                     AFEESRRLGIEPSFKLETRMSGHVAESWVNSQGETVFERGMGGVLITFKESEKGAKSF
                     LAEAGMGKKDLIFDFSLIRTDTPVTCPRKADFLEATVEIPKNTLSPLQSPHQNISPQE
                     RDGKTVWICRIRKADPDVPDLTRQALNAKDRFLFLAPSHHIESDHPEVLKAARKAVEN
                     ARTPREKVERLTSWVANEIKDEPVDSFSALEVLHARKGECQAHTLLYTAMARALGIPT
                     RLTGGIVYMEGMGFLYHAWAESFVDNWIPVDPTFNQVPVDATHIKLVEGPDWLSLLPM
                     GSIIGHIKIRNIRFRCGENSD"
     gene            2276..>2531
                     /locus_tag="PLR47_09890"
     CDS             2276..>2531
                     /locus_tag="PLR47_09890"
                     /inference="COORDINATES: protein motif:HMM:NF016750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HOG13302.1"
                     /translation="MGTIHKTGCVLCAQNCGLEIEVENNRIVKVRGDKSNAKSEGYIC
                     RKGLNVAYHQHNADRLKYPLKKVGDKFERISWDQAIDEIAT"
BASE COUNT          574 a          743 c          695 g          519 t
ORIGIN      
        1 gcccggcgac gcaaggcccg tgttcctttt tctcagccac tttcatctgg atcacattgc
       61 gggccttcac accctgacca aatttctact cggcggcggg ttgaccatct gctgccccgc
      121 cggcggccgg gacgttcttc aaacgttcgt gaatgctccg ttcactgtcc cgctcgaagg
      181 tctgccctat ccggtccgta tgctcgaact gccgggagaa gccgcatccc tgccctttcc
      241 cgttcaggcc ctgccgcttc tccattcagg gctgacgctt ggatatcgtc tggagatcgg
      301 cgcgaaagtc atcgcctact gcccggatac gggttactgc gaaaacgccg tgatcctggc
      361 gcgcggcgcg gatctgctga tcacggaatg cgcctacaaa gaaggtcagt cgtcggagag
      421 ctggcctcac ctgaatcccg agacggccgg ccggatcgcc cgggaagccc gagccctgcg
      481 tctggcgctg actcactttg acgcccgcat ctaccgaagc cttccggaaa gaatggaggc
      541 gcaaaccgcg gcccgaaagg aattccccgc cgcctttgcc gcctgtgacg acatgatcct
      601 tgaatgctga agacgcgcga tgaaatttaa gctttttcca aacacagctc cgggaaaatg
      661 ccttcttgct tcgcgtcatt ttccctgggc ttttgtgcgc cggctgccgt ttttgccggc
      721 ggccgttctg ttcctgacgc tcctcgcgtc ctgcgcaccg caatattttt cacccgtccc
      781 cggggatgct ttcacgcgca aggatctgcg ggagctgtcc ggccgagagc tttggcacgg
      841 gtttgttttc aacggtgaaa aagtggggtt tgcccgcctg aagatagaac cggatgccaa
      901 cgggcaggcc tacacactgt tttccgaggc cagcctgcgc atccgttttc tcgggatggg
      961 caagagcatc agcattcggg gcgaagatcg ggtggccccc gatctttcgc tgatctcttt
     1021 ccaatacgaa cagacgatgg acgacaagcc cctgatcctc aaaggcgaaa tccgtgacgg
     1081 ccgcctgttg gttgttcaac gatcggggag cgaagccaaa accttcgaaa caaagatttc
     1141 ggatccactg tatccggcaa gcggaatcaa cctttatccc gtccttcagg gaatgaagac
     1201 cggcgacgat tacgcctatc cggtctatga tccgcagacg cagtccgtcg tggacgtgtc
     1261 ccagtcggtc gttgcctttg aagaaagccg gcggcttgga attgaaccct ccttcaaact
     1321 ggagacgcga atgtcggggc acgtcgcaga aagctgggtc aattctcaag gagaaacagt
     1381 cttcgagcgc ggcatggggg gcgtgctgat cacgttcaag gaaagcgaaa agggcgcgaa
     1441 gagttttctg gccgaagcag gcatgggcaa aaaagatttg atttttgact tcagcctcat
     1501 ccggacggac acacccgtca cctgtccccg aaaagcggat tttctggagg caaccgtcga
     1561 aatcccgaaa aacaccctgt ctcccctgca aagcccccat cagaatattt ccccgcaaga
     1621 gcgcgacgga aaaacggttt ggatctgccg cattcgcaaa gcggaccccg atgttccgga
     1681 cctgacccgt caggcgctga acgcaaaaga tcgcttcctt ttcctcgccc cttcgcatca
     1741 cattgaaagc gatcatccgg aagttctcaa ggcggcccgg aaagctgttg aaaacgccag
     1801 gacaccccgc gaaaaggtcg aacgcttaac ctcctgggtg gcaaatgaaa tcaaggatga
     1861 accggtggat tctttctctg cgctggaggt ccttcacgcc cgaaagggag aatgccaggc
     1921 ccacaccctg ctttacacgg ccatggcccg agcgctggga atccccacgc gtttgaccgg
     1981 gggcatcgtt tatatggaag gcatgggatt tctctatcat gcctgggcgg aaagctttgt
     2041 ggacaactgg attcccgtgg atccgacgtt caatcaggtt cccgtggacg cgacccatat
     2101 caaactggtg gaaggccccg attggctttc cctcctgccc atgggaagca tcatcggaca
     2161 catcaaaatc aggaacattc ggttccgttg cggcgaaaac agcgattgac aaacgatgca
     2221 ttttcattaa aaggggtata gaacgattgg ccgacaggcc ggaaaaggag atgccatggg
     2281 aacgattcat aaaaccggct gtgtcctctg cgcgcagaac tgcggtttgg aaatcgaagt
     2341 ggaaaataac cgcatcgtca aggtcagagg cgacaaatcc aacgccaaaa gcgaagggta
     2401 tatctgccgc aagggactga acgtcgccta tcaccagcac aacgccgacc gcctgaaata
     2461 tcccctgaaa aaagtcgggg acaaattcga gcggatctcc tgggaccagg ccatcgatga
     2521 aatcgcaacg a
//
LOCUS       DAOSIM010000281         2498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_36240_length_2498_cov_7.084323, whole genome shotgun sequence.
ACCESSION   DAOSIM010000281 DAOSIM010000000
VERSION     DAOSIM010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36240_length_2498_cov_7.084323"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="PLR47_09895"
     CDS             <1..853
                     /locus_tag="PLR47_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG13303.1"
                     /translation="QTKYFFCKTMPQYQKTKRIAFLWEDLAWTTEWRKGISYINLPTW
                     EKMAKDCGLEVVYSKAVKPRGTMYLPILQDIKKSNADLIFYISSWFTDTESFAKQWSD
                     SAARDIQVNLYGGVAQTQDYWKMTGGKGLGVISSYTDLDTIPLTPKTIPLVKKAHARK
                     IPMQIHVHIAYADIYHFKSAIERAKGTGNIKTLIKAMEDVETVYSLGKMKYETRKIKP
                     FYHSRVRVDAANPWKTYPGYFLQLIVQFQEGGKIAFLHESCEENEKLVSKWVDPKRYK
                     TPAELRK"
     gene            1098..1715
                     /locus_tag="PLR47_09900"
     CDS             1098..1715
                     /locus_tag="PLR47_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13304.1"
                     /translation="MPKNTILHALVFVQNWGEEDGLMTFLRGNPHAVSIFHVMGRHSY
                     LIDSNFDDREQLHDWINRLKALRLSSGVPAVISVQTQKVIQVFKQKNDFDLKDYKDLT
                     DRFHFFMLIDNPHHDEKLIELLKKMRIVSSILHVQGANSFIVEIITESYDSYKKMLGK
                     AKELKSIQHIETLEVLSVKKYRNQVIDKGGELSYPQADLRELYTL"
     gene            complement(1774..>2498)
                     /locus_tag="PLR47_09905"
     CDS             complement(1774..>2498)
                     /locus_tag="PLR47_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOG13305.1"
                     /translation="INHPPANAMAQGVLRDLTDLLNRCEKDDSVRVLIITGTGPKCFS
                     AGADITEFADLQSGKVPQYDGNDVYFKIENYPKIIIAAIQGLALGGGLELACSCHLRI
                     MAEEAVVGLPEVKLGLNPGWGGTQRLPRYIGKTKALELMLTGDFMPAKEALALGLLNK
                     VVPAAEVLETAKKLAKKLAGGAPVAQREIMKAVRLGLEGTLYDGITRVEKAAFQTLLV
                     TEDFKEGAKAFLEKRPAQFKGR"
BASE COUNT          587 a          695 c          668 g          548 t
ORIGIN      
        1 ccagacgaag tatttcttct gcaagaccat gccccagtat cagaaaacga aaaggatcgc
       61 tttcctgtgg gaggatctgg cctggacgac ggaatggcgc aaaggcatca gctacatcaa
      121 tctgcccacc tgggaaaaaa tggccaagga ctgcggtctg gaagtggtgt acagcaaggc
      181 ggtcaaaccc cgcggcacca tgtatctgcc gattctgcag gacatcaaaa aatccaacgc
      241 cgatttgatt ttctacatca gctcgtggtt tacggacacg gaatcctttg ccaaacagtg
      301 gtctgattcc gcggcccggg acatccaggt gaatctctac ggcggcgtcg cccagaccca
      361 ggattactgg aaaatgacgg gcggcaaggg cttgggcgtg atcagctcct acacggatct
      421 ggatacgatc ccgctgacgc ccaaaaccat ccccctggtg aaaaaagcgc atgcgcgcaa
      481 aatccccatg cagatccatg tccacatcgc gtatgccgat atttaccatt tcaagtcagc
      541 catcgaaaga gccaagggaa ccggcaacat caaaaccttg atcaaagcga tggaagatgt
      601 ggaaaccgtc tactccctgg gcaagatgaa atatgaaacc cggaaaatta aacctttcta
      661 tcattcccgc gtgagagttg acgcggctaa tccctggaag acttatcccg gctactttct
      721 gcagttgatc gtccagttcc aggagggcgg caagattgcc ttcctgcatg agtcctgcga
      781 ggaaaacgaa aagctggtgt ccaaatgggt ggatcccaaa cgctacaaaa caccggcgga
      841 gttgaggaag taattgatta tagggaacag tcgcgactgt tccctgcaga ctgaaggctg
      901 aaaggaaggg ggcggcattc ttgtgaaggc cgcccccttt tccttttccg ccacggttct
      961 tccgttcccg gcccgccgcg ctcctgaaat gccgcttgac acttccgcgc tttgtttcat
     1021 atacttcgtg ccgtaaaaac aaaagctgtc cggcattcat tttccacgca gccgggacgg
     1081 gattcgaagg aggaagcatg ccgaaaaata cgatattgca cgcgctggtt tttgtgcaaa
     1141 actgggggga agaagacggg ttgatgactt ttctgagagg aaacccgcac gccgtttcca
     1201 tcttccacgt gatgggccgg cacagctacc tcatcgactc caattttgac gaccgggagc
     1261 agcttcatga ctggatcaac cggctcaagg cgctccggct ttcctcgggc gtgcccgccg
     1321 tgatttccgt gcagacgcag aaagtcatcc aggtcttcaa gcaaaaaaac gattttgacc
     1381 tgaaggatta caaggatctg acggaccggt ttcacttttt catgcttatc gacaatcccc
     1441 accatgacga aaagctgatc gaactcctga aaaaaatgcg cattgtcagc tccattctgc
     1501 acgtgcaggg cgcgaactcc tttatcgtgg aaatcatcac ggaatcctac gacagctaca
     1561 aaaagatgct cggcaaggcc aaggagctca agagcattca gcacattgaa acgctggaag
     1621 ttctttccgt caaaaaatac cgcaatcagg tcattgacaa aggcggagag ctttcctatc
     1681 cgcaggcgga tcttcgggaa ctctataccc tgtagtcctc aaaaaaaacg gcggccaaac
     1741 cggccgccgt ttttcgtttt tacgccattt tccctaacgg cccttgaact gcgccggtct
     1801 cttttccaga aacgccttgg cgccttcttt gaaatcttcg gtgaccagca gggtttggaa
     1861 ggcggctttc tcaaccctgg tgatcccgtc gtaaagggtg ccctccaatc ccaggcgaac
     1921 ggctttcatg atctcgcgct gtgccacggg agcgccgccc gccagttttt tggccagttt
     1981 tttggcggtt tccaggactt cggcggcggg gacgaccttg ttgagcaggc cgagcgcgag
     2041 cgcttctttg gccggcatga agtcgccggt gagcatcagc tccagcgcct tggttttgcc
     2101 gatatagcgg ggcaggcgct gagttccgcc ccagccggga ttgaggccga gcttgacttc
     2161 gggaaggccc accaccgctt cttccgccat gattctcaaa tggcaggagc aggccagctc
     2221 caggcccccg cccagggcca ggccctgaat ggccgcgatg atgatcttcg ggtaattttc
     2281 aattttgaaa tatacgtcgt tgccgtcgta ctggggcact ttgcccgact ggaggtcggc
     2341 aaattcggtg atgtccgcgc ccgctgaaaa gcatttcggg ccggtgccgg taatgatgag
     2401 cacgcgcacg gagtcgtcct tttcgcagcg gttgagcagg tcggttaaat cccgcaaaac
     2461 cccctgcgcc attgcgttgg ccggcggatg attgatgg
//
LOCUS       DAOSIM010000282         2493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_36340_length_2493_cov_7.214930, whole genome shotgun sequence.
ACCESSION   DAOSIM010000282 DAOSIM010000000
VERSION     DAOSIM010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36340_length_2493_cov_7.214930"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="PLR47_09910"
     CDS             complement(<1..1293)
                     /locus_tag="PLR47_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG13306.1"
                     /translation="MAIFEYGEYKNTYEAPDNLVDIFENSAANFANNLFIGEKDSSGA
                     YQWATYAQIATRVNHLRAGLASLGVQAGDTVGIIANNRKEWVIGEFATQGLRAAFVPM
                     YEKELVTMWKYIIKDAAVKVLLVSKPEILEKVKNFPAEIPTLQHIYLIEGTGDKTMAA
                     LEKKGAEKPVPSQKPKYSDVAVLIYTSGTTGEPKGVLLSHGNLAENVKAGLAWFLNLT
                     EQSRCISMLPWAHSFGITADLHAFMLRGGSIGIMGSVETLIDDLPKIQPTFMITVPRV
                     FNKVYNGVWAKMREEGGLKLKLFEMALAAAKQKRETGKVGLKYKLLDKIVLAKIRDRF
                     GGRLDNAVTGSAPMNVEIAKFFIDVGIPTYDAYGLSETSPAITINSPLMGNRLGSVGK
                     PINKTKVVIDRSRTGDSGDDGEIICFGPQCMIGYHNKPE"
     gene            complement(1548..>2493)
                     /locus_tag="PLR47_09915"
     CDS             complement(1548..>2493)
                     /locus_tag="PLR47_09915"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOG13307.1"
                     /translation="GLEHPHTPAHLLFANNYYLSALGSMTAPLANRMAASNIGKKILG
                     QMGIATYIPFPKFSFQTLSREKKNRNRGERKVAFFYGCFINFYRPDIGRKIIRMLQSF
                     HINVVLPPQVCCGLPALGNGNLALARTFARKNVASLSNYIDAGYDIVYTCTSCGLCLL
                     QDYQGILEIPEAKKIAENCYDLHEYLLKLIAEREIHPVFREVNRTLAYHIPCHLRALK
                     IGYPAADLMKRIPDLDFTILDDACCGLSGSYGFKKTNEAAALQLGERAVSLVRKTGAA
                     AIISDCGSCRMQMGGLSGMDAFDPAEILCESLGIRDKK"
BASE COUNT          512 a          672 c          702 g          607 t
ORIGIN      
        1 ttccggtttg ttgtggtagc cgatcatgca ctgggggccg aagcagatga tttcgccgtc
       61 gtcgccgctg tcacccgtcc gcgaccggtc gatcaccacc ttggttttgt tgatcggttt
      121 tccgacagac cccaggcggt tgcccatcag cggcgagttg atggtaatgg ctggagacgt
      181 ttcgctcagc ccataggcgt cataggtcgg gatgcccaca tcgatgaaga atttggcgat
      241 ttccacgttc atcggggcgc tgccggtcac cgcgttgtcg aggcgtccgc caaagcgatc
      301 acgaatcttg gccaaaacga ttttgtccag aagcttgtat ttcagaccga ccttgcccgt
      361 ttcccgtttt tgctttgccg ccgccagcgc catttcaaag agcttcagct tcagcccgcc
      421 ttcctccctc attttggccc agacgccgtt atagactttg ttgaacaccc tgggcactgt
      481 gatcatgaac gtcggttgaa tcttgggcag atcgtcgatc aatgtttcga ccgatcccat
      541 gatgccgatg gagcctcccc gcagcatgaa cgcgtgcagg tccgccgtaa tgccgaagga
      601 gtgagcccag ggaagcatgg aaatgcagcg gctttgctcg gtcaggttca aaaaccaggc
      661 cagtcccgcc ttgacgtttt ccgccaggtt gccgtgggaa agcaaaaccc ccttgggctc
      721 tccggtggtt cccgatgtgt agatcagaac ggcgacgtcg gagtatttgg gcttctggga
      781 cggaaccggc ttttcggcgc ctttcttttc cagggcggcc atcgtcttgt cccccgtgcc
      841 ttcgatcaga tagatatgct ggagcgttgg aatttcagcc gggaagtttt tcactttttc
      901 cagaatctcc ggtttggaga cgagcaggac cttgacggcg gcgtccttga tgatatattt
      961 ccacatcgtc accagttctt tttcatacat cggcacgaag gcagctcgca atccctgcgt
     1021 ggcgaattct ccgatcaccc attccttgcg gttattcgcg atgattccga ccgtgtcccc
     1081 ggcctggact cccagcgacg caagaccggc ccgcaggtga ttgacgcggg ttgcgatttg
     1141 cgcgtaggtt gcccactggt acgctccgga agaatctttt tctccaataa acagattatt
     1201 ggcgaagttg gcggcgctgt tttcaaaaat gtccaccaga ttatccgggg cctcatacgt
     1261 gttcttatac tcaccatatt caaaaatcgc catgccctta cccgctcctt tcgaaatttg
     1321 attacgcgtt cgtgtaaaag aatctgtgaa aatgattgta cggttatgct ttgttcgtca
     1381 gtgaatcgag ccggacgctt ctttttccaa caacacaggg ccacatcttg tcgcattcaa
     1441 agaattgaaa tccggaaaga gctctgaagg agcgtagaag cggctgaatg accgtctttc
     1501 cgacggcttc tctataaagg aaaaacagac ggcaagtcaa gataaattta ttttttatcc
     1561 cggatgccaa gcgattcgca cagaatttcc gccggatcaa acgcgtccat tccggaaagg
     1621 ccgcccatct gcatgcggca cgatccgcaa tcggagatga ttgccgcggc gccggttttg
     1681 cgaaccaatg atacggcccg ctcgcccagt tggagagccg cggcttcgtt ggtcttcttg
     1741 aatccgtagc tgccggaaag tccgcagcag gcatcgtcga ggatcgtgaa gtccagatcc
     1801 ggaatccgct tcatcaggtc ggcggccggg tagccgattt tcagcgctcg cagatggcag
     1861 ggaatatggt aggccagcgt gcggttgact tcccgaaaga cgggatgaat ctcccgctcc
     1921 gcgatgagct tgagcaggta ttcatgcagg tcgtagcaat tttccgcgat ctttttcgct
     1981 tcgggaattt ccagaatccc ctggtagtcc tgcagcaggc acaagccgca ggacgtgcag
     2041 gtgtagacga tgtcataccc cgcgtcgata tagttggaaa gggaggcgac gttctttcgg
     2101 gcaaaggttc gcgccagcgc cagattgccg ttgcccagcg cgggcagtcc gcagcacacc
     2161 tgggggggaa ggacgacatt gatgtgaaag gactgcagca ttcgaatgat ttttcgtccg
     2221 atgtcggggc ggtagaagtt gatgaagcag ccgtaaaaga aagcgacctt ccgctctccg
     2281 cggttgcggt ttttcttttc ccggctgagc gtttgaaaac tgaatttggg gaaagggata
     2341 tacgtcgcga tgcccatttg cccgaggatt ttttttccaa tattggaggc ggccatccgg
     2401 ttggccagcg gcgccgtcat cgacccgagc gccgagaggt agtagttgtt ggcaaaaagc
     2461 agatgcgccg gcgtgtgggg gtgttccagg ccg
//
LOCUS       DAOSIM010000283         2489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_36404_length_2489_cov_8.290058, whole genome shotgun sequence.
ACCESSION   DAOSIM010000283 DAOSIM010000000
VERSION     DAOSIM010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36404_length_2489_cov_8.290058"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PLR47_09920"
     CDS             complement(<1..555)
                     /locus_tag="PLR47_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG13308.1"
                     /translation="MRLKDKAAIITGSGRGIGEGIVMRFAQEGAKIIVNDVNEADAMA
                     VVEKVRAGGGQAVAVLGSVSDREVVQKMVDTAVAQFGTLDIIVNNAGITRDVMLHKMT
                     DDQWKAVIDVNLTGVYYGIQCAGRVMREKKYGKIINISSVSALGNAGQLNYSASKAGV
                     IGMTKTAAKELGPKNVNVNAIAPGM"
     gene            complement(629..1396)
                     /locus_tag="PLR47_09925"
     CDS             complement(629..1396)
                     /locus_tag="PLR47_09925"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOG13309.1"
                     /translation="MSDPDIIAFCNQKGGVGKTTCALNIAVGLTLLRKKVLVIDFDPQ
                     AHLTYSLGLDAHNLEGTIYSVMNGAAPLAGAVMEIKGMKVLPANLNLGRLETELSALP
                     KKEMILKNRLADTSDVQYIIIDCPPSSGLLTVNALVACREVFIPLQMEFLALKGMSRL
                     LTLIEDVNKKLNKDGPVYRIIPTRFDARKRLNNAIMDKVRERFGTRVFNTVIRENIAV
                     AEAPSFGLSIFEYAPKSHGAEDYLALCREIIRKRPAG"
     gene            complement(1442..2302)
                     /locus_tag="PLR47_09930"
     CDS             complement(1442..2302)
                     /locus_tag="PLR47_09930"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG13310.1"
                     /translation="MKRDIPELKHQDVGGAELPYLHYPGGEKQMLFCHATGFLPWLWH
                     PIIEEFGPEYSIWAPYICNYRSCDPHQGGLKWNIIAEDLGAFCRAQKIENHVQVGHSM
                     GATVSTIAASLFGVRPRGLFLIEPIFLPEEFYSLTPSVEGHPLASKSIKRLNYWQSEQ
                     DAHAYLKSRSFFTTWDEEILHLYKRFGMEKMPEGDMRLACSPESEAAMFMGGWCCNPW
                     PLLEKIGCPALVLEGEKSPNVDFVDLHRAVSLLPRGEFGSVAGAGHLIPMEKPREITL
                     LIKEFAAKLA"
BASE COUNT          475 a          740 c          695 g          579 t
ORIGIN      
        1 catgcccggc gcgatggcgt tgacgtttac gtttttcggc cccagctcct tggcggcggt
       61 cttggtcatg ccgatcaccc ccgctttcga ggccgagtag ttgagctggc ccgcgtttcc
      121 cagcgcgctc accgaggaaa tgttgatgat cttgccgtac tttttctcgc gcatgacgcg
      181 gcccgcgcac tggatgccgt agtaaacgcc cgtcaggttc acatcgatga cggctttcca
      241 ctggtcgtcc gtcattttgt gaagcatcac gtcgcgggtg atgcccgcgt tgttgacgat
      301 gatgtcgagc gtgccgaatt gcgccacggc ggtgtcgacc attttctgga caacttcccg
      361 gtcggacacg ctgcccagaa cggccacggc ctgcccgccg ccggccctga ctttctccac
      421 gacggccatg gcatccgcct cgttgacgtc atttacgatg atcttcgcgc cttcctgggc
      481 aaaacgcatc acaatgcctt ctccgatgcc gcgccccgat ccggttatga tggctgcctt
      541 gtctttcagt ctcatacgat ctccttttca tttttttcgg tggtcaaccg tttaccggtt
      601 tttcccgttg tttcccttca tgaccccctc agccggcggg ccgcttgcgg atgatttccc
      661 tgcagagcgc cagataatcc tccgccccgt ggcttttcgg agcgtattca aaaatggaca
      721 ggccgaaact gggggcttcc gccacggcga tgttttcgcg gatgaccgtg ttgaacacgc
      781 gcgtgccgaa gcgctcgcgc accttgtcca tgatggcatt gttgagccgt ttgcgggcat
      841 cgaagcgcgt gggaatgatc cgatacacgg gcccatcctt gttcaatttt ttattgacgt
      901 cttcgatcag cgtcagaagc cggctcatgc ctttgagcgc cagaaactcc atctgcagcg
      961 gtataaagac ctcgcggcag gcaaccaggg cgttcacggt cagaagcccc gacgacggcg
     1021 ggcagtcgat aataatatac tggacatcgg aagtgtcggc caaacgattt ttcaggatca
     1081 tttccttttt gggaagcgcg gataattccg tttccagcct tcccagattc agattggcgg
     1141 gcagcacttt catccctttg atttccatga ccgcgccggc aagcggtgcg gcgccgttca
     1201 tgaccgaata aatcgtgcct tccagattgt gggcgtccag tcccagagaa taagtaaggt
     1261 gcgcctgcgg atcgaaatcg atcaccagaa cttttttcct aagcagggtc agaccgacgg
     1321 cgatgttgag ggcgcaggtc gtctttccca cgcctccctt ctggttgcaa aaagcgatga
     1381 tgtcaggatc ggacatgatt caccttcctt tgcgccctcc cgcagccggg ggttccgaat
     1441 ctcaggcaag ctttgccgcg aactccttga tcagcagggt aatttcccgg ggtttctcca
     1501 tcgggatcag atgaccggcg cccgccacgg acccgaactc cccgcgcggc aggagcgaga
     1561 cggcccggtg gagatccacg aaatcaacgt tggggctctt ctccccttcg agcacgagcg
     1621 ccggacagcc gatcttttcc agaagcggcc agggattgca gcaccagccg cccatgaaca
     1681 tggccgcttc actctccggc gagcaggcca gacgcatgtc gccttccggc attttttcca
     1741 tgccgaagcg cttgtacaga tggaggatct cttcatccca ggtcgtaaaa aaggagcggg
     1801 atttcaaata ggcgtgcgcg tcttgctcgc tttgccagta gttgaggcgt ttgatcgact
     1861 tcgacgccag cggatggcct tcgacgctgg gcgtcaggct gtagaattcc tccggcagga
     1921 aaatcggctc gatcagaaac aggccgcgcg gacgaacacc gaaaagggaa gcggcgatcg
     1981 tcgaaaccgt ggcgcccatg gaatggccga cctgcacgtg attctcaatc ttctgcgcgc
     2041 ggcagaacgc gcccaggtcc tcggcgatga tattccactt caggccgccc tgatgcgggt
     2101 cgcatgaccg gtaattgcag atgtagggcg cccagatgga atattcgggg ccgaactcct
     2161 caatgatcgg gtgccagagc cagggcagga aaccggtggc atggcagaag agcatctgct
     2221 tttcgcctcc cggataatgc aggtagggca gttcggcgcc gcccacgtcc tgatgcttca
     2281 attcggggat gtctcttttc atcggcaaag caatcctttt ccagtgaaca ttccgctcgc
     2341 ggggcgcagt cggcatgttt ttttaaaaga cgaatacggc ggcggcaacc accgtcgtga
     2401 aatgattatc catgcatatt ttcgactggg tcacattcag cgtccggacg atcttgccgc
     2461 tgattttgaa gatttccttt ttttcatcc
//
LOCUS       DAOSIM010000284         2487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_36444_length_2487_cov_7.525082, whole genome shotgun sequence.
ACCESSION   DAOSIM010000284 DAOSIM010000000
VERSION     DAOSIM010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36444_length_2487_cov_7.525082"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PLR47_09935"
     CDS             complement(<1..744)
                     /locus_tag="PLR47_09935"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOG13311.1"
                     /translation="MKSIFARRPGAAPFGELPRGQRTAKPSLHFPSRISPGNVVVLRS
                     VNAMDIQYSLIRSRKRKKTLSLQIGSRSEITVQAPYHMPAAEIQRFVKEKQAWIEKSL
                     RRQALQQASHSKKACETGEQFYYLGEACPLEAFFDPLEYPGVVFRDHRFFLNCPADRK
                     LRKYFLTAWYRKQARRHLTQRVDFYSRLLGLTPGGIRITSAEKRWGSCSEKNHLSFAF
                     RLIMAPPQMVDYVVVHEMMHIREKNHSSKF"
     gene            672..1139
                     /locus_tag="PLR47_09940"
     CDS             672..1139
                     /locus_tag="PLR47_09940"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HOG13312.1"
                     /translation="MPSVGREAALRKGPRRACGQRCFSFSGDCDRARKKSREVKPMEE
                     KNLRPWGYYQILSDAADHKVKRILVVPGGTLSLQRHTRRAEHWYAVSGSGAAAIDGKV
                     HPLSAGGAVDIPKGATHRLENTAREDLVIIEVQTGTYFGEDDIERLEDKYGRA"
     gene            complement(1151..>2487)
                     /locus_tag="PLR47_09945"
     CDS             complement(1151..>2487)
                     /locus_tag="PLR47_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOG13313.1"
                     /translation="AGGVEASLRRIAHYDFWSNAVRRSILFDARIDILAYGMAEKAVL
                     EIARRLNAGEDLRNIRGTCVIENEPPPDYLELPSFENAAADKRAFLQMFSRFSANQDP
                     ATAQGLAQRHADRFLVHHSPQPHLTEAELDAVYALPFTRLVHPFYAKQGKVRAIDTIS
                     FSLTTHRGCFGGCRFCSIALHEGRTILSRSEASILQEAREIAARPDFKGVLADVGGAT
                     ANMYGIGCARQPARGACADKECLYPQPCPSLKNDHTRQIRLLAGLRSIPGVKKVFVAS
                     GVRHDLVLADKKHGVSYLRELVKHHVSGQLKIAPEHTAPGVLKLMGKPPAQTLTEFKA
                     LFAKLNNEEKRKQFLTYYFIAAHPGCTEEDMRALQSFAARELHTVLEQVQIYTPLPST
                     WSALMYWTGIDPANGRKIFAEKDPVKKQRQKDMAAARRRFPGAKHQKAGAKK"
BASE COUNT          512 a          683 c          774 g          518 t
ORIGIN      
        1 gaattttgaa gaatgatttt tctcccggat atgcatcatt tcatgaacaa ccacgtaatc
       61 caccatttgc ggcggcgcca tgatcaggcg aaaagcgaag gataaatgat tcttttcaga
      121 gcaggagccc caccgttttt cggcggaggt aatccgaatg ccgccgggcg tgagacccag
      181 aagccggctg tagaaatcca cccgttgggt gagatgccgc cgggcctgct tccggtacca
      241 ggccgtcagg aaatatttac gcagtttccg gtccgcgggg cagttcagaa aaaaacgatg
      301 atccctgaac accactcccg gatactccag cgggtcaaaa aaagcttcca gcgggcaggc
      361 ctcgcccaga tagtaaaact gttcgcccgt ttcacaggct ttcttggaat gcgaggcctg
      421 ctgcagggcc tgccggcgca gggatttttc gatccaggcc tgtttttctt tgacaaagcg
      481 ctgaatctcc gcggccggca tgtgataagg cgcctgaacc gttatttccg aacggctgcc
      541 gatttgcagc gacagggtct tttttcgttt tcggcttcgg atcagcgaat attgaatatc
      601 catggcgttt acggagcgaa gcactaccac attgccgggg gaaatgcgag acgggaaatg
      661 aagagacggt tttgccgtcc gttggccgcg aggcagctct ccgaaagggg ccgcgccggg
      721 cctgcgggca aagatgcttt tcattttccg gggattgtga tagggccagg aaaaaaagcc
      781 gggaagtgaa accaatggaa gagaaaaatc ttcgcccctg gggctactat caaatcctgt
      841 ccgatgccgc cgatcacaag gttaaaagaa ttctggtggt gccgggcggc accctgagcc
      901 tgcaacgcca caccagacga gcggagcact ggtatgcggt ttcgggcagc ggcgcggcgg
      961 cgattgacgg caaagtccat cccctttcgg ccggcggcgc cgtggatatt ccaaagggcg
     1021 ccactcaccg cctggaaaac accgcccgcg aagatctggt gatcatcgaa gtgcagaccg
     1081 ggacctattt cggcgaggat gacatcgaga ggctcgaaga caaatacggc agggcgtaaa
     1141 gaaagggctg ctacttcttc gcgccggcct tctgatgctt tgcgccgggg aaccggcgtc
     1201 gggcggcggc catgtctttc tgcctttgtt ttttgacggg gtccttttct gcaaaaattt
     1261 tccgtccgtt ggccggatcg attcccgtcc agtacatcag cgccgaccag gtggatggca
     1321 gaggcgtgta aatctgcacc tgctccaaaa cggtgtgcag ctcccgcgcg gcgaaggatt
     1381 gaagagcgcg catatcttcc tcggtgcatc ccggatgagc ggcgatgaaa taataggtca
     1441 gaaactgttt tcgtttttcc tcattattca attttgcaaa gagcgccttg aattcggtta
     1501 gcgtctgcgc cggcggtttg cccatcagct tcaaaacgcc cggcgcggta tgctccggcg
     1561 caatcttcag ctggccggaa acgtggtgtt ttacgagttc acgcaggtag gatacgccgt
     1621 gctttttatc cgccagcacc agatcgtggc ggacgccgga ggcgacaaac acttttttga
     1681 cgccgggaat gctgcgcaat cccgcgagga gtcgaatctg ccgggtgtga tcgtttttaa
     1741 gcgaagggca gggctgcgga taaagacact ctttgtccgc gcaagcgccc cgggcgggtt
     1801 ggcgtgcgca accgatgccg tacatgttgg ccgtcgcgcc gcccacgtcc gccagaaccc
     1861 ctttgaagtc cggccgtgcg gcgatctccc tcgcttcctg caaaatggaa gcttccgacc
     1921 ggctcaggat ggtccggcct tcgtgcaatg cgatggagca aaaccggcac ccgccgaagc
     1981 agccgcggtg cgtcgtgagg gaaaacgaga tggtgtcgat ggcgcggact ttgccctgct
     2041 ttgcgtaaaa cgggtgtacg agccgggtga aggggagcgc gtaaaccgcg tccagttccg
     2101 cttccgtcag gtgcggctgc ggggaatggt gaacgagaaa gcggtcggcg tgccgctgcg
     2161 cgagcccctg cgccgtggcc gggtcctggt tggcggaaaa acggctgaac atctgaagga
     2221 aggctctttt atcggccgcc gcgttttcaa aagaaggcag ttccaggtaa tcggggggcg
     2281 gttcgttttc gatgacgcag gttccgcgga tgttgcggag gtcttctccg gcgttgagac
     2341 gccgcgcgat ttccaggacc gccttctccg ccatcccgta ggccagtatg tcgatgcggg
     2401 cgtcgaacag aatggagcgg cggacggcat ttgaccagaa gtcgtagtgc gcgatgcgcc
     2461 gcagactggc ttcgacgccg ccggcga
//
LOCUS       DAOSIM010000285         2482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_36540_length_2482_cov_34.889164, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000285 DAOSIM010000000
VERSION     DAOSIM010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36540_length_2482_cov_34.889164"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PLR47_09950"
     CDS             complement(<1..501)
                     /locus_tag="PLR47_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13314.1"
                     /translation="MGENISDYSQNIVDLRRHMWMAGVEVELRRLIWQIAVVGKYISA
                     KIHESNRKLAGFKNIYGEEQLALDRVSDDILKSKLHHSGFVSHYASEELDDIVAIGNG
                     KEKYIVTADPLDGSSLVDTNLAIGTIIGIHKNSIFDCGRNSMVAALYITYGPLITMIY
                     SAGRGAH"
     gene            complement(987..1403)
                     /locus_tag="PLR47_09955"
     CDS             complement(987..1403)
                     /locus_tag="PLR47_09955"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOG13315.1"
                     /translation="MDAFLEMGRQVLASQPFSVLIGAELTGFAVGSAEIKVPIQPKLK
                     QQHGFLHGGVVSYAADNALTYVGGSVLGPAVVTSEFKINYVRPAKGDYIVARAAAVYV
                     GKNQAVCRCDVFAVKGGEESLCAIAQGTINKLGQSK"
     gene            complement(1421..2419)
                     /locus_tag="PLR47_09960"
     CDS             complement(1421..2419)
                     /locus_tag="PLR47_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13316.1"
                     /translation="MLNVVEDEKLIARYARLFTKSFRPFVDEKIRVKLGHQGANFDAK
                     VAWSKKLNLWIYSQATRGVRYWNVFGRGKPPASGHLTITAEINFPWKGIDRKTGAAFA
                     RDRQNRIYVIHRGKIGGGKKGVGKSLFEKNYRGIWAWMEDGDSLSQVAVVGLLGSERF
                     ALQAAGFVEKIEKLKLAASRSFQTSLDFPEVFFDGLRVGNPPSSAPDEEIYAGDHDLV
                     VGQLASLLSRRKYKIGNDERLELFMMRPASDGVSCLFAVCADRREKSVLAAAAKLLLE
                     KSTRGGHPRAILVLPEEEANRYAEPMRRISIEVFGYRPEGEKILFPDLDLAGFDRD"
BASE COUNT          550 a          735 c          629 g          568 t
ORIGIN      
        1 atgcgcgccc ctgccggccg aataaatcat cgtgatcagc gggccgtagg taatatacag
       61 cgcggcgacc atggaattcc gtccgcagtc gaaaatggag ttcttgtgaa tgccgatgat
      121 cgtgccgatg gccagattgg tgtccacaag ggacgatccg tccagcggat cggcggtcac
      181 gatgtatttt tccttgccgt tgccgatggc cacgatgtcg tccagctctt cggacgcgta
      241 gtggctgaca aaaccggaat gatgcagttt gcttttaaga atatcgtcgc tcacgcggtc
      301 cagcgcgagc tgctcctctc cgtaaatgtt tttaaaaccg gccagtttgc ggttggattc
      361 atgaatttta gcggaaatgt actttccgac aacggcgatc tgccagatca gccttctcag
      421 ctccacttcg acgccggcca tccacatgtg gcgcctcagg tcaacaatgt tttgtgaata
      481 atcactgatg ttctcgccca tagcttctcc ttgatatctt tcgttttttg gtttaccgaa
      541 ccagcaagcg cggtattgtc cgggtccaca ggcatgcagc aaggtttatt aaccaaatat
      601 ccccggccat tacaagcaaa tatttcattc tttcccgttt attgggctga caggaaaaaa
      661 catttttaca tgctttcgcc gcaaaacaaa catcgtgaag catttgcttt cggattccca
      721 atccggtcgg cctgcacgga aaagccggtt gtgcaccatc ttctttttca ttccctgctc
      781 ggcgcagggc aattgtttta tggatttcat ggatcgcaca acaggtctgt atccaataaa
      841 aggaggggaa agcggcagca ttccatgcag ccggaaaaac cggacgaccg gaccgaccct
      901 acggccgccg tcaacaacgt ccggcgcttt ttccatgaag cggccgggcg gctttcattg
      961 atatcaaggc tgtgatccga ctcaggctat ttggactgtc ccaatttgtt aatggtgccc
     1021 tgggcgatcg cgcagagact ttcttcgccg cccttgacgg caaaaacatc gcagcggcag
     1081 accgcctgat ttttcccgac gtaaaccgcc gcggcccgcg cgacaatata gtcacccttg
     1141 gccggccgca cgtaattgat tttgaattcc gacgtcacga cggcggggcc caaaacactg
     1201 ccgcccacat aggtcagcgc attgtccgcc gcgtagctga ccacgccgcc atgcagaaac
     1261 ccatgctgct gtttgagctt tggttggatg ggaaccttga tttcagcgct gcccacggca
     1321 aaaccggtca gctccgctcc gatcaaaaca ctgaagggct gcgaggcaag aacctgccgg
     1381 cccatttcca aaaacgcatc cataaaaaac gtgcctctcg tcaatcccgg tcaaacccgg
     1441 ccaggtccag gtccgggaaa agtatttttt cgccttccgg gcgatagccg aagacttcaa
     1501 tcgatatccg tcgcatgggc tctgcgtaac ggtttgcctc ttcctccggg agaaccagga
     1561 tcgcccgggg atggccgccc cgggtggatt tttccaggag cagcttcgcg gcagcggcca
     1621 gcacactttt ttcgcgccgg tcggcgcaaa cggcaaaaag gcaggatacg ccgtccgagg
     1681 cgggccgcat catgaacaat tccaacctct catcatttcc gattttatat ttccggcgcg
     1741 aaaggaggga agccagctgg ccgacgacca gatcatgatc gccggcatag atctcttcat
     1801 caggagcgga tgaaggcggg tttccgacgc gcaagccgtc aaaaaacact tccggaaaat
     1861 ccagcgacgt ctggaaggag cgggaggccg ccaatttgag cttttcgatc ttttcgacaa
     1921 acccggcggc ctgcagggca aaccgttcgc ttcccaacag tccgaccact gcgacctgcg
     1981 aaagggaatc gccgtcctcc atccaggccc agatcccccg gtagtttttc tcaaacagag
     2041 atttgccgac gccttttttg ccgcctccga ttttcccccg gtgaatcaca taaatgcggt
     2101 tttgccggtc ccgggcaaag gcggcgccgg tcttccggtc gatgcccttc cacggaaaat
     2161 tgatttccgc cgtaatcgtc aggtggcccg acgccggagg cttgcctctt ccgaacacat
     2221 tccagtagcg gacgccccgg gtcgcctgcg agtaaatcca gagattcagc ttttttgacc
     2281 aggcaacttt ggcgtcgaaa ttcgccccct gatgccccag tttcacccga atcttttcat
     2341 cgacaaacgg ccggaagctt ttcgtaaaga gacgcgcata ccgggcaatc agtttttcat
     2401 cttcgacaac gttcagcata tgccctcctt acactaatcc agccgttttt taaagcgggc
     2461 aatcgctccg gcaaacaccg cg
//
LOCUS       DAOSIM010000286         2475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_36678_length_2475_cov_8.664050, whole genome shotgun sequence.
ACCESSION   DAOSIM010000286 DAOSIM010000000
VERSION     DAOSIM010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36678_length_2475_cov_8.664050"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1516)
                     /gene="alaS"
                     /locus_tag="PLR47_09965"
     CDS             complement(<1..>1516)
                     /gene="alaS"
                     /locus_tag="PLR47_09965"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOG13317.1"
                     /translation="GMVQFKNAFLGLENRGYTRAVSCQKCARAGGKHNDLENVGVTAR
                     HHTFFEMLGNFSFGDYFKEEAIAWAWEYLTDVVKLPREKLWVTIFENDDDAFTIWHEK
                     MKVPADRIVRMGEKSNFWMMGETGPCGPCSEIIFDQGEGTGCGRPECDIHCECDRHLE
                     IWNNVFTQFDRDASGKLTELPRPNIDTGMGLERLTAVIQGVKSNYDTDLFAPILRFVE
                     KTSGKAYGKNAESDISIRVIADHSRALTFLIGDGILPSNEGRGYVLRRILRRAARHGK
                     MLGIGKPFLNEAAMVVIDMMKYTYPDLAEKASFITKVILNEEQRFMETLDAGLRILQE
                     EAEALKKAGKTVLQGEMIFKLYDTFGFPTDLTADIVKKDGITLDTAGFEAEMERQRQR
                     ARVAWKGSGEEAVAECYLKASSSGVVSAFCGYEAVRRDEAKVTAIFVDGKSVGAAPAG
                     EKAEIVLDTTPFYGESGGQTGDTGYLEGEGFRFHVENTKKPVENFIVHQGVILSG"
     assembly_gap    1517..1593
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1594..2056)
                     /locus_tag="PLR47_09970"
     CDS             complement(<1594..2056)
                     /locus_tag="PLR47_09970"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOG13318.1"
                     /translation="MKALDLEAAKQKAYRLLSLRPHSERELAKKLLDKGFPKTIVKEA
                     LEKLHELKYLNDASFAGGRARHLAVGKLWGNRKIAACLREKGVDDRLIEDAIALARRE
                     LSEEEAISLWMKKKASKGRADRKEKQKIFQSLMGRGFPPGLILKMLGTPAEE"
     gene            complement(2058..>2475)
                     /locus_tag="PLR47_09975"
     CDS             complement(2058..>2475)
                     /locus_tag="PLR47_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HOG13319.1"
                     /translation="IGVFFGNPETTTGGNALKFYSSQRLDIRKMTSIKNGQDVVGFRT
                     RVKVVKNKMAPPFREAEFDIIFGEGISREGDVLDLAAANGIIEKSGAWYSYKGDRIGQ
                     GRDNSRVFLKENPDVLAAVERDVREKLGIVVKDQEE"
BASE COUNT          431 a          723 c          646 g          598 t
ORIGIN      
        1 tccgctcagg ataacgccct gatggacgat gaaattctca acgggttttt tcgtattctc
       61 cacatgaaaa cggaatcctt cgccttcgag atagcccgta tctcccgtct gtccccccga
      121 ttccccgtaa aacggcgttg tatccaggac gatttccgcc ttttcgccgg ccggcgccgc
      181 tccgacggac ttgccgtcca cgaaaatggc cgtgactttc gcttcgtctc tgcgaacggc
      241 ttcgtacccg caaaaagcgc tcacgacgcc ggagctcgac gccttgaggt agcactccgc
      301 cacggcttct tcgccgctcc ccttccaggc gacgcgggcg cgctggcgct ggcgttccat
      361 ctcggcttca aagcctgccg tgtccagcgt gatcccgtct tttttgacga tgtccgccgt
      421 aaggtcggtg gggaagccga aggtatcgta cagtttgaaa atcatttcgc cttgcaggac
      481 ggtctttccc gcttttttga gcgcttccgc ttcttcctgc aaaatgcgca ggcccgcgtc
      541 gagcgtttcc atgaagcgct gctcttcgtt gaggatcacc ttggtgatga aggacgcctt
      601 ttcagcgagg tccgggtagg tgtatttcat catgtcgatg acgaccatcg cggcctcgtt
      661 taaaaacggc ttgccgatgc cgagcatttt gccgtgtcgc gcggcgcggc gcaggattct
      721 tcgaagcacg tagccgcggc cttcattgga aggcaaaatg ccgtcgccga tcaaaaaggt
      781 gagcgcccgg ctgtgatcgg cgatcacgcg gatggaaata tcgctctcgg catttttccc
      841 gtaggctttt ccggaggttt tttctacgaa acgcaggatc ggcgcaaaca ggtccgtatc
      901 gtagttgctt ttgacgccct gaataacggc cgtgaggcgc tcgagcccca tgcccgtgtc
      961 gatgttgggc ctggggagct ccgtcagttt gcccgacgca tcgcggtcga actgcgtgaa
     1021 gacgttgttc cagatttcca ggtggcggtc gcattcgcag tgaatgtcgc attcgggacg
     1081 cccgcagccc gtaccttctc cctgatcgaa aataatttcg gagcaggggc cgcaggggcc
     1141 ggtttcgccc atcatccaga aattgctttt ttcgcccatg cggacgatgc ggtccgccgg
     1201 cactttcatt ttttcatgcc agatggtgaa cgcatcgtcg tcgttttcaa aaatcgtcac
     1261 ccagagtttt tccctgggaa gcttcacgac gtcggtaaga tattcccagg cccaggcaat
     1321 ggcttcctcc ttgaaatagt cgccgaagga gaagttgccc agcatttcaa aaaaggtatg
     1381 atggcgcgcg gtgacgccga cgttttccag gtcgttgtgt ttgccgccgg cgcgcgcgca
     1441 cttctggcag gagacggccc gcgtgtagcc gcggttttcc aggcccagaa aagcgttttt
     1501 aaactgcacc atgcccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncttcctc cgccggcgtt cccagcatct
     1621 ttaaaatcag gcccggcggg aagccccttc ccatcaggct ttgaaaaatt ttttgttttt
     1681 ctttccggtc cgcccgccct ttggacgcct tcttcttcat ccacagggag atggcctctt
     1741 cttcggacag ctcccggcgc gccagggcga ttgcgtcttc tatcagccgg tcgtccacgc
     1801 ccttttcccg caggcaggcc gcgattttcc ggtttcccca gagtttgccc accgccagat
     1861 ggcgcgcccg gccgccggcg aaagaggcgt cgttgagata cttcaattca tggagttttt
     1921 ccagtgcttc ttttacgatg gttttgggga aacctttatc cagcagtttt ttcgcaagct
     1981 cgcgctccga atgagggcgc agggacaaaa gccgatacgc tttttgcttg gccgcctcca
     2041 ggtccagcgc tttcacgcta ttcttcctgg tctttgacga caatgcccag cttttcccgg
     2101 acatctcttt ccacggccgc cagaacatcc ggattttcct tcaggaaaac ccgggagttg
     2161 tcccgtccct gaccgatcct gtcccctttg taggaatacc aggcgccgct tttttcaata
     2221 atgccgtttg ccgccgccag atcaaggaca tcgccttcgc gggaaatgcc ttcgccgaaa
     2281 atgatgtcga attccgcttc ccgaaagggg ggcgccatct tgtttttcac gaccttgacc
     2341 ctggtccgga atccgacgac atcctgaccg tttttgatgg aagtcatttt gcggatatcg
     2401 aggcgctggg acgaataaaa tttcagcgcg ttgccgccgg tggtcgtctc gggattgccg
     2461 aagaacacgc cgatt
//
LOCUS       DAOSIM010000287         2448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_37183_length_2448_cov_6.463853, whole genome shotgun sequence.
ACCESSION   DAOSIM010000287 DAOSIM010000000
VERSION     DAOSIM010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37183_length_2448_cov_6.463853"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PLR47_09980"
     CDS             <1..630
                     /locus_tag="PLR47_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13320.1"
                     /translation="RLNLYSNVYFHRTTRALDLHLQEIFSETLRLMYPENPSKHLDAY
                     LGCDEWSLFSEVRGWLSVKDARRKKLALEWRKIFARRLKWKMSFAAEISVDSMQRGTE
                     FVGAQNYEARVRECLPASLQRLPFRVDVATQDPRPLNPIAEPGKRINIFNPVTGKTSP
                     EPLNEIYRFIPARVRHFRVFALNHDHDEELARAAESALGNIERSIPTNV"
     gene            complement(690..1454)
                     /gene="ubiE"
                     /locus_tag="PLR47_09985"
     CDS             complement(690..1454)
                     /gene="ubiE"
                     /locus_tag="PLR47_09985"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOG13321.1"
                     /translation="MNTNVPEKYPRVQEISDAQRIRIVKEIFSTVTGKYDFLNRLLSL
                     RRDVAWRKFAAKKMRFFQTRRYLDVACGTGDLSIAAAHMHPDISVTGLDFVNEMVAAA
                     KDKVQKKNLAGRIQIMEGDALHLPFEDNRFDVAGIAFGIRNIPDRARALSEMLRVVVP
                     GGQVMILEMTFVQNRFFKWIYYVYLNFLLPAFAKIFSKNPAAYTYLADSIMNFPSPGQ
                     FAKILEEAGMVHVEKYSLTFGITWLHVGRKAMGNRL"
     gene            complement(1598..2095)
                     /locus_tag="PLR47_09990"
     CDS             complement(1598..2095)
                     /locus_tag="PLR47_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOG13322.1"
                     /translation="MMRIKNLLPTIARPAARDDDHPFYSLQRQMNSLFDDFLAGFEAA
                     PRALSAGGFGAFIPSVDVKESDKDFVIRAELPGVEEKDIEVTITGDSVTIKGEKKEEK
                     EDKGKNYYYMERSYGSFNRVIPLAGETDANKASAGFKNGVLNICIPKIQSAKAKGTKV
                     PIKAG"
BASE COUNT          529 a          674 c          641 g          604 t
ORIGIN      
        1 cgcctgaatc tctacagcaa cgtgtatttt caccgcacga cgcgcgcgct ggatctgcac
       61 ctgcaggaaa ttttttccga aacgctgcgc ctgatgtatc cggaaaatcc atcaaagcat
      121 cttgacgcat acctgggctg cgacgagtgg tcgctgttca gcgaggtgcg gggctggctt
      181 tccgtcaaag acgcgcgccg caaaaaactg gctctggagt ggcggaagat ttttgcaagg
      241 cggctgaagt ggaagatgtc ttttgccgcc gaaatctccg tcgattcgat gcagcggggg
      301 acggagtttg tcggcgccca aaactatgag gcgagagttc gggaatgcct gcccgcctcg
      361 cttcagcgcc tgccgtttcg cgtggacgtt gccacgcagg acccgcgtcc gctcaatccg
      421 atcgccgaac cgggcaaacg catcaatatc ttcaatcccg tcacgggcaa aacgtcaccc
      481 gaaccgctca atgaaatcta ccggttcatt ccggcccgcg tccggcactt ccgcgtgttc
      541 gcgctgaatc atgatcatga cgaggagctg gcccgggcgg ccgaatcggc gctgggcaac
      601 atcgaacgct ccattccgac gaatgtgtga gaaacaggcg aggggccagg ggcgataggc
      661 cataggcaac aaatcctata gcccatagcc tatagcctat tgcccattgc ctttcttccc
      721 acatgcagcc aggtgatgcc gaaggtcagg gaatattttt ccacatggac catgcccgct
      781 tcttcgagaa tttttgcaaa ttgtccggga gacgggaaat tcatgatgga atcggccagg
      841 taggtgtagg cggcggggtt tttggaaaag atttttgcaa acgcgggcag cagaaaattc
      901 agataaacat aataaatcca tttgaaaaaa cggttttgga caaaggtcat ctccagaatc
      961 atgacctgtc cgccggggac gacgacgcga agcatttcgg aaagcgcgcg ggcgcggtcg
     1021 ggaatgttgc ggatgccgaa ggcgatgccc gcgacgtcga agcggttgtc ttcaaaaggc
     1081 agatgaaggg cgtcgccttc catgatttga atccgtccgg caaggttttt cttctgaacc
     1141 ttatctttgg ccgccgccac catctcgttg acaaagtcaa ggccggtgac ggaaatatcc
     1201 ggatgcatgt gggccgccgc aattgaaaga tcccccgttc cgcaggcgac gtccagatac
     1261 cgccgtgttt gaaagaaccg catttttttt gcggcgaatt tgcgccaggc cacgtcgcgc
     1321 cgaaggctca gcagacggtt taaaaagtcg tattttccgg tcacggtgga gaaaatctct
     1381 ttgacgatgc ggatgcgctg ggcgtcggag atttcctgaa cgcgcggata tttttcgggg
     1441 acgttcgtat tcataaaaca ggttcctgtc gtttttcctg ccgcggattt agcagtccgg
     1501 gcggggaaat gcaacaagaa agcaacgagt aaaaaaaccg ccgcccgtca ggtgaagggc
     1561 ggcggtaatc catgttgtct tgaacttcag ccttcttcta ccccgctttg atgggaacct
     1621 tggtcccttt ggctttagcc gactggattt tcggaatgca aatgttcagg acgccgtttt
     1681 taaagccggc ggacgccttg ttcgcgtcgg tttccccggc cagagggatc acgcggttga
     1741 aagagccgta agagcgctcc atgtagtaat aatttttccc cttgtcttcc ttttcttcct
     1801 tcttttcgcc tttgattgtt actgaatcgc cggtgatcgt tacttcaata tctttttcct
     1861 ccacgccggg aagctccgcg cggatcacaa aatccttgtc gctttccttg acgtccaccg
     1921 acgggatgaa cgctccgaat cctccggcgg aaagcgcgcg cggcgcggct tcaaatccgg
     1981 caaggaaatc atcaaacaaa ctattcattt ggcgctgcaa agaatagaag ggatgatcgt
     2041 catctcttgc cgcaggcctt gcaatggtgg gcagcagatt ttttattctc atcatcatat
     2101 cctcctttct tttggatcta aagggtcggc gtcgggcact gtcatggttc gacaagctca
     2161 ccactcgaca ggctcatcat gacacgctgt attgttaccc taatcaaatc gaaaggtatg
     2221 agccatgagc ttttccctta ttacccatta cctctgacct atggcccctc gcctctcttt
     2281 tcactcatca ctttccgctt ttcacttctt gcctatcgcc catggcctct cgcctgtttg
     2341 tttaccatcc actacgttat gccgccttga tcgcaatctt tttcggtttc acttcttccg
     2401 ccttcggcag ctccagtctc agaacgccgt tcttgacggt cgcgacga
//
LOCUS       DAOSIM010000288         2426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_37621_length_2426_cov_9.045129, whole genome shotgun sequence.
ACCESSION   DAOSIM010000288 DAOSIM010000000
VERSION     DAOSIM010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37621_length_2426_cov_9.045129"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PLR47_09995"
     CDS             <1..335
                     /locus_tag="PLR47_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOG13323.1"
                     /translation="PFCRAHMAQLRRDYDSFVKLDTEILVAGPETPETFRDYWQKHDL
                     PFVGLPDPEHRVLKLYGQEVKLFKLGRLPAQMLIDRTGKVRFVHYGHSMADIPPNEEI
                     LHLIETQL"
     gene            438..1253
                     /locus_tag="PLR47_10000"
     CDS             438..1253
                     /locus_tag="PLR47_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG13324.1"
                     /translation="MKNLKGRNVLLTGAGSGIGRLMAHYVADEGANTAIIDIQAEALA
                     KTAKEVRLRKVKVKEYVCDISDCVSVGDVVARIKQDFGQIDVLINNAGTVIGKSFFDL
                     TLEEMQRTMNINYWGHIYLTKAVIGDMVARNDGAIVNVASSSGLIGMPILSDYAASKF
                     AEVGFSESLRRELKKFNYTGVTVTCVCPYIIDTGLFKGFKPMAMSPFIKPEVAARAIV
                     EGLKKGKPYVMLPPLSIYSSLMLKLLPTPLFDWILKMSGAEKAMDCFEGRKSC"
     gene            1290..>2426
                     /locus_tag="PLR47_10005"
     CDS             1290..>2426
                     /locus_tag="PLR47_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOG13325.1"
                     /translation="MNLEFANIGEMVQTQGRRYPDRIAIRFYERDITYRELDEQSNRV
                     ANGLLRLGVRKGDRVCMLMDNSPEFYYTYFGINKIGAIAGPVNCWWQIAEIRYLLKDS
                     GAVVLFCDHTYRGHIDKIIGETPDLQHVIEREAADDRFLPFEKMLTEPVALADAAISP
                     DDVSTIVYTSGTTGSPKGVLLTHRNILTNSWQASLLANIDEKDVILCFLPLFHVNGLV
                     ITGTSPLSKGSQIVLRRNFSASEFWECVAKYRVSIFSGVPTVYQILLNTPGFEGLDVS
                     SLRYGVCGAAPMPVETIRKFEETFNMIIVEGYGLTESTAGATANPINGVRKIGSIGIP
                     FAGTEIRILDDEDKEVPQGDVGEICIRGDHVMKGYFNKPEDTAQT"
BASE COUNT          588 a          646 c          679 g          513 t
ORIGIN      
        1 gaccgttctg ccgcgcgcac atggcgcagt tgcgccggga ttacgattcg ttcgtgaagc
       61 tcgatacgga aatccttgtt gcaggccctg aaacacccga gacattccgc gactactggc
      121 aaaaacatga cctgccgttt gtcggccttc cggatccgga gcaccgggtt ctgaagcttt
      181 acggccagga agtgaagctc ttcaagcttg gccgtctgcc cgcgcagatg ctgatcgacc
      241 gcacgggaaa ggtccgcttc gtgcactacg gccactccat ggcggacatt ccccccaatg
      301 aggagatact ccatttgatt gaaacgcagc tatgaagcaa caccctcata gaccgaactg
      361 ccgggaaaag agtcgatgcc ccaaaccgat gaaaaaaaat aaatgatgag gccggaataa
      421 ttcagcagga agtgcgaatg aaaaatctga aaggcaggaa tgttctcctt acgggcgccg
      481 gaagcggcat aggtcggctg atggcccact atgtggccga tgagggcgcc aatacggcca
      541 taatcgatat tcaagcagag gcccttgcga aaacagccaa ggaggttcgg ctgcggaaag
      601 tcaaggtgaa agagtatgtt tgcgatatat ccgattgtgt atccgtcgga gatgtggttg
      661 cccggatcaa gcaggatttc ggacaaattg acgtgctcat caacaatgcc ggcactgtaa
      721 tcggaaaatc atttttcgat ctgacgctgg aagagatgca gcgcaccatg aatatcaatt
      781 actgggggca catctattta accaaggccg tcatcggaga catggtggcg cgcaatgacg
      841 gggcgattgt caatgttgca tcctccagcg gtcttatcgg catgccgatc ctgtcggatt
      901 acgccgccag caaattcgcc gaagtcggat tctccgaatc gctgcggcgg gaactcaaaa
      961 aattcaatta caccggcgtc acggtgacct gcgtttgccc gtacatcatc gacaccggtc
     1021 tgttcaaggg tttcaaaccc atggcgatga gcccttttat caagcctgag gtcgcggcgc
     1081 gtgcgattgt ggaagggctc aagaagggca agccttatgt catgctcccg cctttgagca
     1141 tttacagttc gctgatgctg aaacttctgc ctacacccct gttcgactgg atcctgaaga
     1201 tgtccggcgc cgagaaggcg atggactgct ttgaaggcag aaaatcatgt taaattagaa
     1261 atgttgatca tcatggaaag gagatcagca tgaatctcga atttgcgaac atcggggaaa
     1321 tggtgcaaac gcagggtcgc cgctatccgg acagaatcgc catccgcttt tacgaacggg
     1381 acatcacgta tcgtgaactg gatgaacaga gcaaccgcgt ggccaacggt cttcttcggc
     1441 tgggcgtccg aaaaggcgac cgggtctgca tgctcatgga taacagcccg gaattttact
     1501 atacttattt tgggatcaac aagatcgggg ccatcgccgg tccggtcaac tgctggtggc
     1561 agatcgcaga aatccgttac ctgctgaaag attccggagc ggtggtcctg ttttgcgacc
     1621 acacctaccg ggggcacatc gataagatca tcggtgaaac gccggatctg cagcacgtca
     1681 ttgaacggga agcggccgac gatcgttttt tgccgttcga aaaaatgctg acggagccgg
     1741 tggctcttgc ggatgcggcg atcagccccg acgacgtctc cacgatcgtc tatacctcgg
     1801 gaaccaccgg aagcccgaag ggagttttgc tcacccacag gaatatcctg accaattcct
     1861 ggcaggccag tctgctggcc aacatcgatg aaaaagacgt cattttgtgc ttcctgccgc
     1921 tcttccacgt caacggcctc gtgattaccg ggacatctcc cttgagcaaa ggttcacaga
     1981 tcgtcctgcg ccgcaacttc tccgcctcgg agttctggga atgcgtcgcg aagtatcgcg
     2041 tctcgatttt cagcggcgtg ccgacggttt accagattct cctgaatacg ccggggtttg
     2101 aaggattgga cgtgtcgtcg ctgcgctacg gcgtttgcgg cgcggctccc atgcccgtgg
     2161 agaccatccg gaaatttgag gaaaccttta acatgattat cgtcgaggga tacgggctga
     2221 ccgaaagtac cgccggggcg acagccaacc ccatcaacgg cgtccggaaa atcggctcca
     2281 tcgggattcc ctttgcggga accgaaatcc ggattctgga cgatgaggac aaggaggtcc
     2341 cccagggaga cgtgggagaa atttgcattc ggggcgacca tgtcatgaaa ggctacttca
     2401 acaaaccgga ggacaccgcc cagacc
//
LOCUS       DAOSIM010000289         2426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_37623_length_2426_cov_8.397301, whole genome shotgun sequence.
ACCESSION   DAOSIM010000289 DAOSIM010000000
VERSION     DAOSIM010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37623_length_2426_cov_8.397301"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLR47_10010"
     CDS             <1..226
                     /locus_tag="PLR47_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13326.1"
                     /translation="AKATLCKVTLTETEKELCLDVTDNGIGITQWQVDSPRSFGMIGM
                     RERAHLWCGTVQIQNVKPSGTAVRVLIPLQ"
     gene            242..874
                     /locus_tag="PLR47_10015"
     CDS             242..874
                     /locus_tag="PLR47_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG13327.1"
                     /translation="MIRILVADDHAVVRQGVVQILAEVKDMLVKDEAQNGSETLDKVL
                     KYEYDVVLLDISMPGRSGLEVLEDIKAQRPKLAVLILSMHPEEQYAVRALRAGASGYL
                     TKASAPQELIGAIRKVAGGGKYVTASLAEKLAEELDIDTRKLPHERLSNREHQVMMML
                     ASGKTVSDIADELCLSVKTISTYRTRILAKMGMKKNAELTLYAVHNKLIN"
     gene            1235..1624
                     /locus_tag="PLR47_10020"
                     /pseudo
     CDS             1235..1624
                     /locus_tag="PLR47_10020"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
     gene            1719..>2426
                     /locus_tag="PLR47_10025"
     CDS             1719..>2426
                     /locus_tag="PLR47_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOG13328.1"
                     /translation="MDARMFVREPVHTETTTVTGAQNLHDFFYPKSIAVIGASDVKGK
                     LGYNVFSNLVSHQYPGKLYPINPGKETVMGVRAYRSILDVPDPVDLAIVIVPAKAAET
                     AIADCCAKGVRFVVVEAAGFAESGKEGKEAQARIEAIIRKHGCRVLGPNCSGVINTHH
                     NMVQSIGLLGDLKKGNVGMVAQAGVYAAGILTGLSNVLDFGIVATIGNKMDINETDIL
                     EYLSDDDHISVIVMYMED"
BASE COUNT          556 a          728 c          719 g          423 t
ORIGIN      
        1 cgccaaagcg acgctctgca aggtgacgct gacggaaacg gaaaaggagc tttgcctgga
       61 cgtgacggac aacgggatcg gcatcacgca gtggcaggtg gactcccccc ggtccttcgg
      121 gatgatcgga atgcgcgagc gggcgcatct ctggtgcggc accgtccaga tccaaaacgt
      181 caagccgtcc ggaaccgccg tgcgggtgct gattccgctt cagtaaagga gagctgcaaa
      241 catgatacgc atactggtcg ccgatgatca cgccgtagtg cggcagggcg tggtccagat
      301 tctcgcggaa gtgaaggata tgctggtcaa ggacgaagcc cagaacggct cggaaacgct
      361 ggacaaggtc ctgaagtatg aatatgacgt ggtcctgctt gacatctcca tgccggggcg
      421 aagcggcctg gaggtcctgg aggacatcaa ggcccagcgg cccaaactgg ccgtactgat
      481 tctgagcatg caccccgagg agcagtacgc ggtgcgcgcg ctcagggcgg gcgcgtccgg
      541 atatctcacc aaggccagcg cgccgcagga gctgatcggc gccatccgca aggtggccgg
      601 gggcgggaag tacgtcacgg cgtcgctggc ggaaaagctg gccgaggagc tggacatcga
      661 cacccgcaag cttccccacg agaggctctc caaccgcgaa caccaggtca tgatgatgct
      721 ggcttccggc aaaaccgtat ccgacatcgc cgatgaactg tgcctgagcg tgaaaacgat
      781 cagcacctac cgcacccgca ttctggccaa gatgggcatg aagaaaaacg cggagctcac
      841 cctttacgcc gtccacaaca aactcatcaa ctagaagcgc aaggcgcgga tttatccctt
      901 ttcagaatca tttcaaccat ctccgattca ctccgtttca tccgttgtcc gacgatttgt
      961 cagacaaaag ccgtcagcgc ttcctgcatc gttccgacag aacattcagc tccctgctga
     1021 taggatcctt ttttttcctg tgctacacca ccatcaaagg aaacggcaaa aatcgcgacc
     1081 ggaaaccatc acggcacaag gagggtttat gacaagggga tcttcgacat cacagcggca
     1141 aaagacggcg gcgtccaacc aggacggcaa gctgctcagc gacgaactgg cggacatcat
     1201 ccatcaggcc gtccgggaca accgcagtta cctcatggag aacgagagca aggaaattct
     1261 cgaaggcgcg ggcattgcga ccacgggcta cctggtggcg cgctccgaag acgaagcgct
     1321 ggcgctgtgt gaaaaaatcg gctttcccgt cgccatgaaa atcgtttcgc ccgatgtcgt
     1381 gcataaatcg gacgcgggcg gcgtccggct gaatcttcac accgccgacg acgtgcgcaa
     1441 gacctaccgc gccatggtgg aaaccttcaa ataccagcac atcgagggcg tctcgattca
     1501 gcgcatggca gcttccggca ttgaggccat catcggcgtg acgcgcgatc ccagcttcgg
     1561 cccgctcatc atgttcggac tgggcggcgt gtttgtcgaa gtgcttcgcg acgtgtcgtt
     1621 tcgggttctg ccgatcaccg aaaaggacgc ggcggaaatg atcgaagaaa tccgcggctc
     1681 tgaatccgct gtttctctat tccgaaggct acatcgccgt ggacgcccga atgttcgtcc
     1741 gcgaaccggt gcacacggag accaccaccg tcacgggcgc gcagaacctc cacgatttct
     1801 tctatcccaa aagcatcgcc gtgatcggcg catcggacgt caagggaaaa ctgggctaca
     1861 acgttttcag caacctggtc agccaccagt atcccgggaa gctctacccg atcaaccccg
     1921 gcaaggaaac ggtgatgggt gttcgcgcct accggtcgat cctggacgtg ccggatccgg
     1981 tggacctggc catcgtgatc gttcccgcca aggcggccga gacggccatc gcggactgct
     2041 gcgccaaggg cgtccgtttc gtcgtcgtgg aggcggcggg attcgcggaa agcggaaaag
     2101 aagggaagga agcccaggcg cgcatcgaag ccatcattcg aaagcacggc tgccgcgtgc
     2161 tggggcccaa ctgctccggc gtgatcaaca cccaccacaa catggtgcag tccatcgggc
     2221 tgctcgggga tctgaagaag ggcaacgtcg gcatggtcgc ccaagccggc gtttacgccg
     2281 ccggcatcct tacgggactg agcaacgtgc tggacttcgg catcgtggcc accatcggca
     2341 acaagatgga catcaatgaa acggacattc tcgaatacct gagcgacgac gatcacattt
     2401 ccgtcatcgt catgtacatg gaagac
//
LOCUS       DAOSIM010000290         2418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_37792_length_2418_cov_7.506136, whole genome shotgun sequence.
ACCESSION   DAOSIM010000290 DAOSIM010000000
VERSION     DAOSIM010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37792_length_2418_cov_7.506136"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="PLR47_10030"
     CDS             complement(<1..1105)
                     /locus_tag="PLR47_10030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOG13329.1"
                     /translation="MSDAVIVEAVRSPGGRYKRGGLAQTRSDAYGIQVVKGLLARVPE
                     VKPEEIDDLIVGCAFPEAEQGMILGRTLAMGAGLPISCSGMTVNRFCSAGIQAIADAT
                     AKIRAGWSDIIIAGGCETMSHIPMGGTAMRPNPDWKFDGSMPNLWVNMGNTAENVAEH
                     YNISREDMDKMGVESNRRAHEAIKAGKFKDEIIPIQAFKYKIKNGKRIRETVTFDTDD
                     GVRWPAKMEDMAKLKSPFRLGGNCTAANSSQMTDGAAFSMLMSAEKAKELKLKPIARL
                     AYFAVAGVAAEEMGVGPAYAIPKVLKMAGLTPKDIDVYEINEAFASQAIYSCRVVGIE
                     DRYWAGDINPNGGAIALGHPLGCTGAKLTAQLLH"
     gene            complement(1188..>2418)
                     /locus_tag="PLR47_10035"
     CDS             complement(1188..>2418)
                     /locus_tag="PLR47_10035"
                     /inference="COORDINATES: protein motif:HMM:NF027439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOG13330.1"
                     /translation="GYNNKLGPFEAWDAVGVKEAVEVMKKLKLKVPKKIDEMLKKGCE
                     SFYATKADGKYYYDFEKKGYAKIDENPKIILLPDFKSRNKIVKENESATLIDIGDGVV
                     CLEFHTKMNSIDEGLNQMLNDSCDIVEKDFEGMVVANHDERAFSAGANVFAVLIAAQK
                     GEWDLLEKSIEALQNGNMRMKYLEKPVVTAPAGLALGGGCEIAMHGARCLPNGETYMG
                     LVEVGVGVIPAGGGCKELLVRLTEGLPDGVVANGSNLQWYMAKAFENIATAKVATSAM
                     EAMELGYIRKSEKINMSRDQQIYEAKQIALSLVMAGYKPPRPALIPVMGENLRGFAEG
                     VLMNMRYGKYISDYDLFVSRKVAYVLSGGDCAEGTYVSEQEILDLEREAFLSLMGQTK
                     THERIMHMLTKGKPLRN"
BASE COUNT          511 a          685 c          583 g          639 t
ORIGIN      
        1 catgcagcaa ctgagcggtc aattttgcgc cggtgcatcc cagcggatgg ccgagggcaa
       61 tcgcgccgcc gttgggattg atgtcacccg cccaatagcg gtcttcgata ccgaccacgc
      121 ggcaggaata aatagcctgt gacgcaaagg cttcattgat ttcatacaca tcaatgtctt
      181 tcggcgtcaa gccggccatt ttcaggacct tcggaatcgc gtaagcaggg ccgacaccca
      241 tttcttccgc cgcaacgcca gcaactgcaa aatatgccag tctggcaatg ggtttcagtt
      301 tcaattcctt cgctttttcc gccgacatca gcatggagaa ggctgcgccg tctgtcatct
      361 gtgaagagtt ggcagcggtg cagtttccgc ctaaccggaa gggcgacttc agttttgcca
      421 tgtcttccat ctttgcaggc caacgcacac cgtcatccgt gtcaaacgtg acggtttcgc
      481 gaatgcgttt gccattttta atcttgtatt tgaaggcctg aatgggaatg atttcatcct
      541 tgaactttcc ggccttgatg gcttcatgag cgcggcgatt gctttccaca cccatcttgt
      601 ccatatcttc acgcgaaata ttgtaatgtt cggctacatt ttcggccgtg ttgcccatat
      661 taacccagag attgggcatg ctgccgtcaa atttccaatc cgggttcgga cgcatggcgg
      721 taccgcccat gggaatatgg ctcatggtct cgcagccacc ggcaataata atatccgacc
      781 agccggcgcg gatttttgcc gtagcgtcgg caatggcctg aataccagcc gaacaaaaac
      841 ggttgacggt catgccggag catgaaatgg gcaagcccgc tcccatcgcc agagtccgcc
      901 ccagaatcat gccctgctcg gcttccggaa acgcacaacc aacgatcaaa tcatctattt
      961 cttcgggttt tacttccgga actctcgcca gaaggccctt gactacctga atgccatagg
     1021 catccgacct ggtttgtgca agaccacctc ttttataacg gccgcctgga cttcttacgg
     1081 cttcaacaat tacagcatca ctcatggttt tcctccttca tttctagaag gcgcggaggc
     1141 ttttctgaac tttttttcaa accgccgcgc tcattatttc ataatagtta gttgcgcaac
     1201 ggtttgcctt ttgtcagcat gtgcatgatg cgttcgtgcg ttttcgtctg tcccatcagg
     1261 ctcaggaaag cttctctttc cagatccaga atttcctgct ccgaaacata ggtgccttcc
     1321 gcacagtcgc cgccggagag aacataagcc acttttctgg atacgaacag atcataatca
     1381 gaaatgtatt tgccgtatct catgttcatc agtacgcctt cagcgaagcc gcggagattt
     1441 tctcccatca cgggaatcaa agcgggtctg ggcggtttgt aaccggccat caccagggac
     1501 aaagcaatct gtttggcttc gtagatctgc tgatcacggc tcatgttgat tttttccgac
     1561 ttgcggatgt agcccagttc catggcttcc atcgcgctgg tggctacttt cgccgtggct
     1621 atgttttcaa aagctttagc catgtaccac tgcagattgg acccgtttgc cacaacgcca
     1681 tccggaaggc cttcggtcaa gcgaaccaga agttccttgc agccgccgcc cgcgggaatc
     1741 acgccgacac cgacttcgac cagacccata taggtctcgc cgttgggcag acagcgggcg
     1801 ccgtgcatgg cgatttcgca gccgccgccc agagccaggc cggcaggagc ggttacaacg
     1861 ggcttttcaa ggtatttcat tctcatattg ccgttctgca gggcttcaat gctcttttcc
     1921 agcagatccc actcgccctt ctgagcagcg atcagaaccg cgaaaacgtt ggcgcccgcg
     1981 gagaatgccc gctcgtcgtg attggcgacc accatgcctt cgaaatcttt ctcgacgata
     2041 tcgcaggaat cgttgagcat ctgattcaag ccttcgtcaa tcgaattcat cttggtatgg
     2101 aattccaggc agaccacgcc gtcgccgatg tcaatgaggg tggcgctttc attttctttg
     2161 acaatcttgt tgcggctctt gaaatccggc aacaggatga ttttgggatt ttcgtcaatc
     2221 ttggcatagc ccttcttttc gaaatcgtag taatatttgc cgtccgcctt ggttgcgtag
     2281 aaggattcac aacctttttt cagcatttca tcgatctttt tggggacttt cagcttcagt
     2341 ttcttcatca cttcgacggc ttccttgacg ccgacggcat cccaggcctc gaagggaccc
     2401 agcttgttgt tgtagccc
//
LOCUS       DAOSIM010000291         2407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_38046_length_2407_cov_7.826105, whole genome shotgun sequence.
ACCESSION   DAOSIM010000291 DAOSIM010000000
VERSION     DAOSIM010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38046_length_2407_cov_7.826105"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PLR47_10040"
     CDS             complement(<1..737)
                     /locus_tag="PLR47_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOG13331.1"
                     /translation="MEVFQRLFTMARQYYARFILAAICMIVAGGLQSALPLIAKPAID
                     EIFMSKNIAALKWIPLAVVAIFLFKGLANYGQNILMSSIGLRIVTNLRNQLYEQIQKQ
                     SLSFFAEHPTGLLMSRITNDVQSVQTASSEAITALVKDTFMLICLVGVVFYTDWKLAL
                     IAMVVFPLTIYPISRFGKKMRKVTTSSQITMGTLNSLLQETISGTRIVKAFCMEKYEN
                     DRFAAENERLFKYSMKAVSVNAISSPL"
     gene            complement(746..1897)
                     /gene="lpxB"
                     /locus_tag="PLR47_10045"
     CDS             complement(746..1897)
                     /gene="lpxB"
                     /locus_tag="PLR47_10045"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOG13332.1"
                     /translation="MNKNSGSAPRVMIVAGEASGDLHGALLVREMLAVNPSVEFFGIG
                     GNQMREAGVKLLAHAADIAVVGVSEVFSKTAVLLRTISRMKKSLDRLKPNLVILIDFP
                     DFNLNIAARAAKKRGIRVFYYISPQVWAWREGRVEQIKKLVDKMAVILPFEVDFYAAH
                     GFDVHWVGHPLLDIVKTTFTAQEARAHFGLSANKTTIGLLPGSRNSEIQKLLPEMIQA
                     AQIISQKFPTAQYILPLAETLEQKTVAGFLQGSGLNIQIVAGRTYDVLACCDLALVTS
                     GTATLETGLMGVPMVIVYKVSLFSELIGRMIIRQQHIGLVNILAGKTVAPELIQRDAR
                     GPRIASEALAILQNREKRQQIIRELGNIRATLGEPGAAGRAAQIACNMI"
     gene            complement(1920..2150)
                     /locus_tag="PLR47_10050"
     CDS             complement(1920..2150)
                     /locus_tag="PLR47_10050"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOG13333.1"
                     /translation="MIPMVDLKRQYARLKKEIDPKITPKTKAIIPVHRFGHPADMAPV
                     MDIAREVLSLPMFPELTGEEIRQAAEAIRHVC"
     gene            complement(2147..>2407)
                     /locus_tag="PLR47_10055"
     CDS             complement(2147..>2407)
                     /locus_tag="PLR47_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG13334.1"
                     /translation="GYHSVDCRKREVLSFSKITGDDGKPQIVQNALELGGHDPLEEEI
                     RSFVEAVRGRSEPRVSGEDGRKSLALAIEILQKMKTGEGLRL"
BASE COUNT          506 a          664 c          685 g          552 t
ORIGIN      
        1 atcagcgggc tggaaatggc gtttacggaa acggccttca tgctgtattt gaacagacgc
       61 tcgttttccg cggcgaaccg atcgttttca tatttttcca tgcagaaggc ttttacgatg
      121 cgcgtgccgg aaatggtttc ctgcaggagc gagttcagcg ttcccatggt aatctgcgag
      181 gaggtggtca cctttctcat cttttttccg aaacgggaaa tcggataaat cgtcagcgga
      241 aaaacaacca tggcgatgag cgcgagcttc cagtcggtat aaaaaacaac tcccaccagg
      301 cagatcagca tgaaggtgtc cttgaccagc gccgtgatgg cctccgacga cgcggtttga
      361 acggactgca cgtcgttggt aatccgcgac atcagcaatc cggttggatg ttcggcaaaa
      421 aaggataaag actgcttctg aatctgctca tagagctgat tgcgcagatt ggtgacgatg
      481 cgcaaaccga tggagctcat cagaatattc tgtccgtagt tggccagccc cttgaacaga
      541 aaaatcgcga cgacggccaa gggaatccac ttcagggcgg cgatgttttt gctcatgaaa
      601 atttcgtcga tggcgggctt ggcaatcagc ggcagagcgg attgcagccc tccggcgaca
      661 atcatgcaga tggcggccag gataaaacgg gcgtaatact gccgggccat tgtaaataat
      721 ctttgaaata cttccatggt tcgtcctata tcatattgca ggcgatttgc gcagctcttc
      781 ctgcggcgcc gggttcgccg agtgtggcgc gaatgttgcc cagctcccga atgatctgct
      841 gccgtttttc ccggttttgt aaaatcgcca gagcctccga cgcgatgcgc ggaccgcggg
      901 cgtctcgctg aatcaattcc ggtgcgaccg ttttcccggc cagaatgttg accagtccga
      961 tgtgctgctg ccggatgatc atccggccga tcagctccga aaagagggag accttgtaaa
     1021 cgatgaccat cggaacgccc atcaggcctg tctccagcgt tgccgttccg gaggtcacga
     1081 gcgccagatc gcagcaggcc agaacgtcat aggtgcgccc ggcgacgatt tgaatattca
     1141 ggccggagcc ttgcaaaaag ccggcgacgg ttttttgctc cagggtttcc gccaggggca
     1201 ggatgtactg cgccgtggga aatttttgag aaatgatttg cgccgcctga atcatttccg
     1261 gcagcagctt ctgaatttcc gaattccggc ttccgggaag caggccgatg gtcgttttgt
     1321 ttgccgaaag gccgaagtga gcgcgggcct cctgagccgt gaaggttgtt ttcacgatgt
     1381 cgagcagggg atgcccgacc caatggacgt cgaatccgtg cgcggcgtaa aaatccactt
     1441 caaacggcag aatcaccgcc atcttgtcca ccagtttctt gatctgttca acgcgccctt
     1501 cacgccaggc ccacacctgc gggctgatgt agtaaaagac ccgaatgcct ctttttttgg
     1561 cggcgcgggc cgcgatgttc agattgaaat cgggaaaatc gatcaggatg accaggttgg
     1621 gcttcagccg gtccagggat ttcttcattc gggaaatggt cctgagcagg acggcggttt
     1681 ttgaaaacac ttccgacacg ccgaccaccg caatatccgc cgcgtgcgcc agcagcttca
     1741 cgcccgcttc ccgcatttga ttgccgccga tgccgaaaaa ttccacagag ggattgacgg
     1801 ccagcatttc ccggacgagc agggcgccat gcagatcgcc ggaggcctct ccggcaacga
     1861 tcatcactct gggagcgctt ccgctgtttt tgttcatgat ggccttcctc ccgcccggtt
     1921 cagcaaacat gacggatcgc ttcggcggcc tgccggattt cttccccggt cagttccgga
     1981 aacatcggca gggaaagaac ctcgcgcgcg atgtccatca ccggggccat gtcggcggga
     2041 tggccgaacc ggtggacggg aatgatcgct tttgtttttg gagtgatttt cgggtcgatt
     2101 tcttttttca gacgggcata ctgtcttttc aaatccacca tcgggatcat aggcgcaggc
     2161 cctcgccggt tttcattttc tgaagaatct cgatggccag cgccagagac ttgcggccgt
     2221 cctctccgga cacgcgcggc tcagagcggc cccgcacggc ttcgacaaac gagcggattt
     2281 cttcttcgag ggggtcatgg ccgcccagct cgagagcgtt ttgaacaatt tgcggtttgc
     2341 cgtcgtcgcc cgtgattttg gaaaacgaca gaacctctct tttccggcag tccacggagt
     2401 gatagcc
//
LOCUS       DAOSIM010000292         2360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_39012_length_2360_cov_6.090239, whole genome shotgun sequence.
ACCESSION   DAOSIM010000292 DAOSIM010000000
VERSION     DAOSIM010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39012_length_2360_cov_6.090239"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1938)
                     /gene="lon"
                     /locus_tag="PLR47_10060"
     CDS             complement(<1..1938)
                     /gene="lon"
                     /locus_tag="PLR47_10060"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOG13335.1"
                     /translation="MTEEKDNTRTGTSNIIPLLPLRDVVVFPHMIVPLFVGREKSIAA
                     LESAMKYEKGIFMVAQKNAKKDDPSEDDIYSVGTIGIIIQLLRLPDGTVKVLVEGKAR
                     GAILEYVKNDDFFLVRVADIEDVDDDPNDVRKQALMRSIKESFELYLKLSKKVHVEMM
                     GTIAGIEDPSKLADVVVTHLNVKLEDKQRIMEIFSIGERLEAIYSLMLSEIEILQVEE
                     KIKRRVKKQMEKTQKDYYLNEQMRAIQKEMGEKDDFKNEIAELEKRLKQKKLSEEAAK
                     KVRQEIKKLQMMAPMSAEATVVRNYIDWLLDMPWSEVTENQYTLKESEAILDEDHYGL
                     KKVKERIIEYLAVQSLVKKNKGPILCLVGPPGVGKTSIAKSVARATNRKFIRISLGGV
                     RDEAEIRGHRRTYIGAMPGKIIQSLKKAGSNNPVFCLDEVDKLCSDFRGDPSSALLEV
                     LDPEQNVAFNDNYLDVDYDLSDILFITTANVLQTIPAPLQDRMEVIRIAGYTEQEKMQ
                     IAKRFLLAKEMEANGLTPESIEFTNQALLRIIRQYTREAGVRNLEREIASICRKVAKE
                     IVSNGNRRKIVISSKNIPKYLGVPKFRHGETEENSQVGLTTGLAWTEFGGELLVIEAS
                     IMEGTGRMVMTGKLGDVMQESV"
     gene            complement(1964..>2360)
                     /locus_tag="PLR47_10065"
     CDS             complement(1964..>2360)
                     /locus_tag="PLR47_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOG13336.1"
                     /translation="VRSEDLLKFGLIPEFIGRLPVIATLDDLEEAALVRILVEPKDAI
                     IKQYKKLFELENVNLKFTDGALRCVAKLSVERKSGARGLRSILENTMLEVMYDIPSQR
                     DIKECVISEEVVLNQEKPILIYDQKSESA"
BASE COUNT          441 a          685 c          619 g          615 t
ORIGIN      
        1 caccgattcc tgcatcacgt cgcccagctt gccggtcatg accatgcggc ccgtcccttc
       61 cataatggag gcctcgatga cgagcagttc gccgccgaac tccgtccagg ccaggcccgt
      121 cgtcaaaccg acctggctgt tttcctccgt ttcgccgtgg cggaattttg gaacgcccag
      181 gtatttcggg atatttttgg agctgatgac aattttcctg cggttcccgt tgctgacgat
      241 ttccttggcg accttgcggc agatggacgc aatttcccgc tccaggttgc gcacgccggc
      301 ttcccgcgtg tactggcgga taatcctgag cagggcctga ttggtgaatt cgatggactc
      361 cggcgtcagg ccgttggctt ccatttcctt ggcgagcagg aaccgcttcg cgatctgcat
      421 tttttcctgc tccgtgtagc cggcaatgcg gatgacttcc atccggtcct gcaggggcgc
      481 cgggatcgtc tgcagaacat tggccgtcgt aatgaacaaa atatcggaca aatcataatc
      541 cacgtccaga taattgtcgt taaacgccac gttctgctcg ggatccagaa cttccagaag
      601 cgccgaagag ggatcgccgc gaaaatcgga gcagagcttg tccacttcat ccaggcagaa
      661 aacgggattg ttggatccgg cctttttcag cgattgaatg atcttgccgg gcatggcccc
      721 gatgtaggtc cggcgatggc cgcggatttc ggcttcgtca cggacgccgc ccagggagat
      781 gcggataaat ttccggttcg tcgcgcgcgc gacggatttg gcaatcgacg tttttcccac
      841 gccgggaggc ccgacgaggc agagaatcgg ccccttgttt ttcttgacca gcgactgaac
      901 cgccaggtat tcgatgatcc gttccttgac ctttttcagg ccgtagtgat cctcgtcgag
      961 aatcgcctcc gactccttga gggtatactg gttctccgtg acctccgacc agggcatgtc
     1021 cagaagccag tcaatgtaat tgcgaacgac ggtggcctcc gccgacatgg gcgccatcat
     1081 ctgcagtttc ttgatttcct ggcggacctt tttggcggct tcctcggaga gctttttctg
     1141 cttcaggcgc ttttccagct ccgcgatttc atttttaaag tcgtctttct cgcccatttc
     1201 cttctggatg gcgcgcatct gctcgtttaa gtagtaatcc ttctgcgtct tttccatctg
     1261 cttcttgacg cgccgcttga tcttttcctc cacctgcaga atttcgatct cggagagcat
     1321 cagcgaataa atcgcctcca gcctctcccc gatgctgaag atttccatga tccgctgctt
     1381 gtcttcaagc ttgacgttca gatgggtcac cacgacatcg gccagtttgg aagggtcttc
     1441 gatgccggca atcgtcccca tcatttccac gtggactttc ttgctgagct tcagatacag
     1501 ctcgaaggat tccttgatgc tgcgcatcag cgcctgtttc cgcacatcgt tcgggtcatc
     1561 atcgacatct tcgatatccg cgacacggac gaggaagaaa tcgtcgtttt tcacgtattc
     1621 gagaatggcg ccgcgcgcct ttccctccac cagcaccttc accgtgccgt ccggcagccg
     1681 caaaagctgg atgatgatgc cgatggttcc taccgaataa atatcgtctt ccgacggatc
     1741 gtctttcttg gcgtttttct gggccaccat gaaaatccct ttttcatact tcatggcgga
     1801 ttccagggcg gcgatggact tctcccgccc gacaaacagc ggaacaatca tatggggaaa
     1861 cacaaccaca tcgcgaagcg gcaaaagcgg aatgatgttg gatgtgccgg tacgggtgtt
     1921 gtctttttct tcggtcattt tctcttcctg cggcggttat gccttatgcg gattcggatt
     1981 tctgatcata gatcaaaatc ggtttttcct ggttgagcac cacttcttcg ctgatgacgc
     2041 attcctttat gtcccgctgc gacggaatgt cgtacatcac ttccagcatg gtgttttcca
     2101 gaatggagcg caggccgcgc gcgccggatt tcctctccac cgacagtttg gccacgcagc
     2161 gcaaggcgcc gtccgtaaac ttgaggttca cgttttccag ctcaaagagc ttcttgtact
     2221 gcttgatgat ggcgtccttg ggctccacca gaatgcggac cagcgccgct tcttccaggt
     2281 cgtcgagcgt ggcgatcacg ggcagacgtc cgatgaattc gggaatcagg ccgaatttga
     2341 gcaggtcttc ggaccggact
//
LOCUS       DAOSIM010000293         2347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_39302_length_2347_cov_13.102967, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000293 DAOSIM010000000
VERSION     DAOSIM010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39302_length_2347_cov_13.102967"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2347
                     /locus_tag="PLR47_10070"
     CDS             <1..>2347
                     /locus_tag="PLR47_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG13337.1"
                     /translation="TNPDGSIVRIRDVGRTELGTESYDIEGTYNGKPAAGLAIRQAPG
                     ANALDTADAVKAKLEEMSQYFPHGMKVVYPYDTTPFVRVAIDEVVKTLFEAILLVFLV
                     MYLFMGNFRATFIPTIAVPVVLLGTFAVLGAFGFSINMLTMFAMVLAIGLLVDDAIVV
                     VENVERIMTEEGLSPREATAKSMDQITSALIGIGLVLSAVFGPMAFFPGSTGVIYRQF
                     SVTIITSMLLSVVVALILTPVLCASLLKPVSNGHEPAENAIWFLRPFFSWFDRNFFWL
                     RDRYLDVVKHSLAKKIRYMLIYAGIVILIALLFVRLPTGYLPDEDQGVLMAQILMPTG
                     STLEQTREVSEEVQRYFLEEEKEVVESYMLVSGIGFSGRAQNNGMVFIKLKDWNLRKG
                     KAAKVKAIAERAMRHFSTNRKAVIFAFPPPAVVELGNAQGVDFQLLDRGGLGHAALMN
                     ARNQLIGMASKDPRLASVRPNGMEDVPEFRIDVDWEKAGELGLPIHSIHNTISATFGG
                     AYANDFIQGGRVKRVYVQADAPYRMLPKDLEKIYVRNTAGKMAPFTSFASTRWTFGSP
                     QLERFNGFPSLNIWAEPAAHHSSGEAMQALEELSGKLPKGIGYDWTGLSYQERMATSQ
                     GPLLYIFSILVIFLCVAALYESWSIPFVNLLMLPLGVFGALAATYFRGLPNDVYFQIG
                     FLTTMGLSTKNAILIVQFIEEQMHQGQGLVEATLSAVRIRFRPVIMTSLAFFFGTLPL
                     AVARGAGAGAMNAIGTAVTGGMVSATFIDLIFIPLFFVVISR"
BASE COUNT          458 a          564 c          702 g          623 t
ORIGIN      
        1 accaatcccg acggatcgat tgtcaggatc agggacgtgg ggcggacgga actgggaacc
       61 gagtcctacg acattgaagg tacctacaac ggcaagccgg ctgccggttt ggccattcgc
      121 caggcgccgg gagcgaacgc tctggatacg gccgatgcgg ttaaagcgaa gcttgaggaa
      181 atgagccagt attttcctca cgggatgaaa gtggtttacc cgtacgatac cacgccgttt
      241 gtgagggtgg caattgatga ggtggtcaag accttgtttg aagccattct gctggtcttt
      301 ctggtcatgt atctttttat ggggaatttt cgggcgacgt tcattccgac catcgcggta
      361 ccggtggtcc ttttaggtac ttttgccgtg ctcggagcct tcggattttc cattaacatg
      421 ttgacgatgt ttgccatggt tttggccatc ggtcttttgg tcgacgacgc gattgtggtg
      481 gttgagaacg tggaacggat catgacggag gaaggactgt ctccgcgtga agctacggcc
      541 aaatcgatgg accagattac gagcgccctg atcgggatcg ggctggtgct ttcggcggtt
      601 ttcggtccca tggcattttt cccgggttcg accggcgtga tttaccgtca gttttccgtg
      661 accattatta cttccatgct gctatcggtt gtggtggcgc tgatcctgac tcccgttctt
      721 tgcgcctcat tgcttaaacc ggtgtccaat ggacacgagc ccgcggaaaa tgcgatctgg
      781 tttttgcggc ctttcttttc gtggtttgac aggaactttt tctggctccg ggatcgttat
      841 ctggatgtgg tgaagcattc gctcgcgaaa aaaatacgct atatgctcat ttacgccgga
      901 atcgtgattt tgatcgcgct tctttttgtg cggttgccga ccgggtattt gccggatgaa
      961 gatcagggcg tattgatggc ccagattctc atgccgaccg gctcgaccct ggaacagacc
     1021 agagaggtat cggaggaagt tcaacggtat tttctggaag aggaaaaaga agtcgtggaa
     1081 tcctacatgt tggtttcggg cattggattt tcggggaggg cgcaaaacaa tggcatggtt
     1141 ttcattaaac tgaaagactg gaatttacgc aaaggcaaag ccgcgaaggt gaaggccata
     1201 gcggagaggg ccatgcggca tttttcgacg aatcgcaaag ccgtgatctt tgctttcccg
     1261 ccgcccgcgg tcgttgaatt ggggaacgct caaggggttg actttcagtt gctggatcgt
     1321 ggtggtttgg ggcatgcggc cctgatgaac gcgcgcaacc aactgatcgg gatggcgtcg
     1381 aaggaccccc ggctggccag tgtccggccg aacggcatgg aggacgtgcc ggaattccgg
     1441 attgacgttg actgggaaaa agccggagaa ctggggctgc cgatccactc aatccataac
     1501 acgatttccg ccacttttgg aggagcgtat gccaatgatt ttatccaggg cggccgggtc
     1561 aagcgggttt acgtccaggc ggacgcgccc tatcgcatgc tccccaaaga tcttgaaaaa
     1621 atttatgtgc gtaacacggc cggtaaaatg gctcccttta cgtccttcgc ctcgacccgc
     1681 tggaccttcg gttctcctca gcttgagcgg ttcaacggtt ttccttccct gaatatctgg
     1741 gcggaacctg ccgcgcatca tagttcgggt gaggcgatgc aagcgctcga ggagctgtcc
     1801 gggaagctgc cgaaagggat tggttacgac tggacggggc tttcctatca ggaaaggatg
     1861 gcgacatctc aagggccgct tctctatata ttttctattc tggttatttt tctgtgtgtg
     1921 gcggcgcttt atgaaagctg gagtattcct ttcgtgaatc ttttgatgct tcctctcgga
     1981 gtgttcggcg cgttggccgc gacgtatttt cgggggctgc cgaacgatgt ctatttccag
     2041 atcgggtttc tgacgacgat gggactttcg accaaaaacg cgatcctgat tgtccagttt
     2101 atcgaagaac aaatgcacca ggggcagggg cttgttgagg caactctgtc ggcggtcagg
     2161 atccggtttc ggcccgttat catgacctcg ctggcttttt tctttgggac actgcctctt
     2221 gccgttgcca ggggagcggg cgccggcgcc atgaacgcga tcggtacggc cgtaaccgga
     2281 ggtatggtat ccgctacttt tattgacctg atttttattc cgctgttttt cgtggtgatc
     2341 tcccgtg
//
LOCUS       DAOSIM010000294         2329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_39700_length_2329_cov_7.227353, whole genome shotgun sequence.
ACCESSION   DAOSIM010000294 DAOSIM010000000
VERSION     DAOSIM010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39700_length_2329_cov_7.227353"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="PLR47_10075"
     CDS             complement(<1..162)
                     /locus_tag="PLR47_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG13338.1"
                     /translation="MAYVITDECIACGSCEDECPVGAITEGEDKYVIDAKLCTDCGAC
                     CDQCPVEAIV"
     gene            complement(222..443)
                     /locus_tag="PLR47_10080"
     CDS             complement(222..443)
                     /locus_tag="PLR47_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOG13339.1"
                     /translation="MKEQVQKALDKVRPGLQADGGDVELIDVSPEGVVKVKLTGACRG
                     CPMSQMTLKMGIEKILKQQVPSVKEVVSA"
     gene            complement(627..1073)
                     /locus_tag="PLR47_10085"
     CDS             complement(627..1073)
                     /locus_tag="PLR47_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13340.1"
                     /translation="MMKKVCARFLLVVAVLSVIACGGLRYSQLDPAAKDYHPKRIAVF
                     PADVGTYEEARNAIEQIVPGVLMETKWFANVIDTATLQRQIQANEELRKISMEYISKL
                     QTLNYSDPALSRKIGELSGTDGFLLVAVDYWNYTVEKRNWPRSASA"
     gene            complement(1091..>2329)
                     /locus_tag="PLR47_10090"
     CDS             complement(1091..>2329)
                     /locus_tag="PLR47_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOG13341.1"
                     /translation="AQTTQDSDHYREIVRKIQKKNPRAVVFNTICSSTEKRQEEIISL
                     AAEMDALFVVGGKNSANTRRLADLARKQNTTTFHIETVRELKNIDLGPYKSIGVSAGA
                     STPNWIIDQITDHLAEKKSRPNKKTAFLLNLWLWMVKTDFYSALGAGCLALAGMLLQN
                     IPLSVASIAVASFFVYAMHVLNRLVTSKESGLIGSFREPFYLRHEKMFCLAAFASLLV
                     ALLLSLTGSIYAFGLLLFISAAGGIYNMKLLPGNRRFGRLRDIPGSKNFFTAVAWGIV
                     TAVLPALSRGWDFSAGMAVAFIFTFVLVFIRSAMSDIMDMQSDRLLGRETIPVIIGKE
                     KTQALLKIILLILLAILIIAPIAGWSPTLSIFLVLCVLYVWICFGLCDRRAGFSGATI
                     EGLLETSYIIAGFAVLGCKT"
BASE COUNT          511 a          656 c          615 g          547 t
ORIGIN      
        1 aacgatcgct tccaccgggc attgatcgca gcaggcgccg cagtccgtgc acagtttcgc
       61 gtcaatcaca tacttgtctt cgccttcggt tatcgctccc accggacact cgtcttcgca
      121 tgagccgcag gcaatgcatt cgtctgtaat cacgtaggcc atatttttaa aaatctcctt
      181 tacgtcaaaa ttttacgcgt ttaccgcttt taagccggcg ctcaggccga cacgacttct
      241 ttgacggaag gcacctgttg cttcagaatc ttttcgatgc ccattttcag cgtcatctgc
      301 gacatcgggc agccgcggca ggctccggtc agcttgacct tgaccacgcc ttccggagaa
      361 acgtcaatca gttcaacatc gccgccgtcg gcctggagcc cgggacggac tttatccagc
      421 gctttttgca cctgttcttt cataatgcat ctccgttttt taaaggttcc ggtcagcgga
      481 ccgccgtcaa tgcggcattt cgtcgaccat atcctgaacg acatcgcggg cgacgtcagg
      541 cagatccggc ttgatcatca tgtagctttt ccccagatga tggccggcct tccacatgat
      601 tttccccgtg gccggatcaa tcagcttcag gccgacgctg accttggcca gtttcttttc
      661 tccaccgtgt aattccagta gtccacggcg accagcaaaa agccgtccgt gccggagagt
      721 tcgccgattt tcctgctcag cgccggatcg gagtagttca acgtctgaag cttggagatg
      781 tattccatcg agattttgcg cagttcttcg ttggcctgaa tctggcgctg cagggtggcc
      841 gtgtcgatga cgttcgcaaa ccattttgtt tccatgagca cgccgggcac aatctgctcg
      901 atggcattgc gcgcctcttc ataggtgccc acgtcggccg ggaacacggc aatccgcttg
      961 gggtgataat cttttgccgc cgggtccagt tgggaatatc gcagtcctcc gcaggcgatg
     1021 acgctcaaaa cagcaacgac cagaagaaac cgtgcgcaca cttttttcat catgatcctt
     1081 acccccttct ttatgttttg cagcccaaaa cggcaaaacc ggcaatgata tagctggttt
     1141 ccagaagccc ttcaatggtg gccccggaaa accccgccct tctatcacaa agcccaaaac
     1201 aaatccatac atataatacg cataaaacaa ggaatatact caacgttggg ctccagccgg
     1261 cgatgggcgc tatgattaaa atcgccagta atatcaagag gatgattttc agaagggcct
     1321 gcgttttttc ctttccgatg atgacgggga tcgtctcccg ccccaaaagc ctgtcgcttt
     1381 gcatgtccat gatatccgac atggccgagc ggataaacac caggacaaac gtgaaaataa
     1441 aggcgaccgc catcccggcg ctgaaatccc agccgcggct caaggcgggc agaaccgccg
     1501 tgacgatgcc ccacgcgacc gccgtaaaaa aatttttgga accgggaatg tctctcaggc
     1561 gcccaaagcg ccggttgccc ggcaggagtt tcatgttgta gataccgccg gccgctgaga
     1621 taaaaagcag cagtccgaac gcatagatgc tgcccgtcag tgacagcagg agggcgacga
     1681 gcagagacgc aaaggccgcc agacaaaaca ttttttcgtg ccgcagatag aatggctcgc
     1741 ggaacgagcc gatcaggccg gattcttttg aggtaaccag gcggttgagc acgtgcatgg
     1801 catagacaaa aaaggaagcg acggcgatgg acgcaaccga gagggggatg ttttgcagaa
     1861 gcattcccgc aagcgccaga cagcccgcgc ccagggcgga gtagaagtcc gtttttacca
     1921 tccacagcca gagattcaga agaaaggccg tttttttgtt tggacggctt ttcttttccg
     1981 ccaaatgatc cgtgatctgg tcgatgatcc agttcggagt ggaggcgccg gcggacacgc
     2041 cgatgctttt gtacgggccg agatcaatgt ttttcaactc tctgacggtt tcgatgtgaa
     2101 aggttgtcgt gttttgtttc cgggcgaggt cggccaggcg gcgggtgttc gcgctgtttt
     2161 tgcctccgac gacaaacagc gcgtccattt ctgcggccag ggaaataatt tcctcctgcc
     2221 gcttctccgt ggaggagcaa atcgtgttga acacgacggc ccgcggattt tttttctgaa
     2281 ttttccggac gatttcccgg tagtggtcgc tgtcctgcgt ggtctgggc
//
LOCUS       DAOSIM010000295         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_40043_length_2313_cov_6.987600, whole genome shotgun sequence.
ACCESSION   DAOSIM010000295 DAOSIM010000000
VERSION     DAOSIM010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40043_length_2313_cov_6.987600"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PLR47_10095"
     CDS             <1..652
                     /locus_tag="PLR47_10095"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOG13342.1"
                     /translation="HVVPSVKGALRCKKAGVDVIVASGHEGGFHTSWEPVHSMILLPA
                     VVDAVSDENTLVAGAGGFCDGRTLAAALVLGADGAQMGTRFLATKESDFHQIWKEGVV
                     AAQDRGTLVARGFVGPARWLKTPRSEEHAQNTLKKSPGVFLGTPDDYSTTDMSLIEFE
                     IESIKAVYAGDREKALMAAGEVAQRINDMPSVNDMVQGIVRDAEATLRGIEKKHLA"
     gene            complement(709..1335)
                     /locus_tag="PLR47_10100"
     CDS             complement(709..1335)
                     /locus_tag="PLR47_10100"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG13343.1"
                     /translation="MTRKTVAGKEETPRKSSALFLKRQSQIIKKATKLFMKQGYAQTS
                     MREISKATGIDIRNLYYFIRSKEEILFLVIEMIHRRELELFEKQGFTSIEDPVLQLRT
                     VVREFVDAGYGYGQEILLLYRESKSLPNRLLKVILSRESQVVARIEEILQRGRENNVF
                     RFEDASFTANMIVYELSLHALRNWNMKQYSKEELTDLLEKHVIKAVTA"
     gene            1537..>2313
                     /locus_tag="PLR47_10105"
     CDS             1537..>2313
                     /locus_tag="PLR47_10105"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOG13344.1"
                     /translation="MKIQDVKNVCVVGAGNMGHQIALQCALSGFKTVSTDVVPAVLEK
                     AQKFVEGYLPGRVQKGKMTEEAAKKARSLISFTGDIQEAAKDADIVIEAVLERIDLKR
                     KVFADLDKYAPPSAILATNSSFIVSSRIADATKRPSQVCNLHFFNPALVMKLVEVVQG
                     PHVSDETAQCMMDFCLKIDKIPIHIKKEVDGFVLNRIFAVIQREALWMLEMGVASFED
                     IDKACVYGAGHPMGPFRLNDLTGIDLTYDICMSHFLESGNP"
BASE COUNT          526 a          636 c          657 g          494 t
ORIGIN      
        1 gcacgtggtt ccttccgtga agggcgcgct gcgctgcaaa aaggcggggg tggatgtgat
       61 tgtcgcttcg ggccatgaag gcggatttca tacctcctgg gagccggtcc attccatgat
      121 cctgcttccc gccgtggtgg atgccgtgtc cgacgaaaac acgctggtcg cgggcgcagg
      181 cggattctgc gacggccgca cgctggccgc cgcgctggtt ctgggcgctg acggcgcgca
      241 gatgggcacg agattcctgg cgacgaagga aagcgatttt catcagatct ggaaagaggg
      301 cgtggtcgcc gcccaggacc ggggaacgct ggttgcccgg ggcttcgtgg gaccggcccg
      361 gtggctgaaa actccccgca gcgaagagca cgcccaaaac acgctgaaga aatcccccgg
      421 cgtctttctg ggaacgcccg acgattactc caccacggac atgtcgctca ttgagttcga
      481 gattgaatcc atcaaggcgg tttatgcggg cgaccgggaa aaggcgctca tggccgccgg
      541 cgaagtggcc cagcggatca acgatatgcc ctcggtcaac gacatggtgc agggcattgt
      601 ccgggatgcg gaggcaacgc tgcgcggcat cgagaagaag catctggctt aacaaaataa
      661 aaaagccggg aggcgactcc cggctttttg acccccgcgg ggccggcgtt acgccgtcac
      721 cgctttgatc acatgctttt caagcagatc ggtgagttcc tccttggaat actgcttcat
      781 gttccagttg cgcagcgcat ggagggacaa ttcatacaca atcatattgg cggtaaagga
      841 cgcatcctcg aaacgaaaca cgttattctc ccggccccgc tgcagaatct cctcgatgcg
      901 ggcaaccacc tggctttccc gggaaagaat caccttgagc agacgattgg gaagggactt
      961 cgactcccgg tacaaaagaa ggatttcctg cccgtatcca tagccggcgt cgacgaactc
     1021 gcggacaacg gttctgagct gcagcacggg gtcttcgatg ctcgtgaatc cctgcttttc
     1081 gaaaagctcg agctcacggc ggtggatcat ctcgatgacc agaaaaagaa tctcctcctt
     1141 gcttcggata aaatagtaca ggttccgaat gtcgatgccc gtggccttgg aaatttcgcg
     1201 catggacgtc tgcgcatagc cctgcttcat gaaaagcttc gtggcttttt taatgatctg
     1261 cgactgtctc ttgagaaaca aagcgctgct ttttctcggc gtctcctcct tgcctgcaac
     1321 ggtttttctg gtcatggatg gcacctgggt cctttcactg aattgaagct tccttacaac
     1381 agaaccggtc tttctgtcaa tgtaaattga aaatattact tgacaaatcg acaccggcaa
     1441 aataattgtt acaaccaacg atggctgtag gaaaatgatt cctggccgcc gttttataaa
     1501 ggaaaaatcc gccaaaacaa agaaggagga acgaaaatga aaatccagga tgtaaaaaat
     1561 gtttgcgtcg tcggggcggg caacatgggg catcagattg cgctccagtg cgctctttcc
     1621 ggttttaaga ccgtcagcac ggatgtcgtg ccggccgttc tcgaaaaggc ccagaagttt
     1681 gttgaaggct atctgccggg ccgcgtgcag aaggggaaaa tgacggagga agctgcgaaa
     1741 aaggcccgca gcctgatatc ctttaccggc gacatccagg aagccgccaa agacgcggac
     1801 atcgtcatcg aggccgtcct ggagcgcatc gacctgaagc gcaaagtatt tgccgatctg
     1861 gacaagtatg ctccgccttc agcgatcctg gcgaccaaca gctcctttat cgtgagctcc
     1921 cggatcgccg atgccaccaa acgcccttcc caggtctgca atcttcactt cttcaatccg
     1981 gcgctggtca tgaagctggt cgaggtcgtt caggggccgc atgtttccga cgaaacggcg
     2041 cagtgcatga tggatttctg cctgaaaatc gataaaattc ccatccatat caagaaggaa
     2101 gtggacggct tcgtcctgaa ccggattttc gccgtcattc agcgggaagc cctgtggatg
     2161 ctggagatgg gcgtggcctc tttcgaagac atcgacaagg cctgcgttta cggagccggc
     2221 catccgatgg gtccgttccg cctcaacgac ctgaccggaa tcgatctgac ctacgacatc
     2281 tgcatgagtc atttcctgga gtccggcaat ccg
//
LOCUS       DAOSIM010000296         2289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_40603_length_2289_cov_8.620859, whole genome shotgun sequence.
ACCESSION   DAOSIM010000296 DAOSIM010000000
VERSION     DAOSIM010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40603_length_2289_cov_8.620859"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PLR47_10110"
     CDS             <1..558
                     /locus_tag="PLR47_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG13345.1"
                     /translation="AGRPVRVDVRLVSATNTNLETACQEGKFRMDLMYRLKSACVCLP
                     PLRERRGDIPLLASHFLKKACARHQKNITGFSPEAMQRLTHGDYPGNIRELSQMVENA
                     ALLAEGPVILPADLGGNGVVLEPQGDAFSRRLCTLKENDSAHVRFVMDATGGNRREAA
                     QILGITLRQLQRKLADMKESFRNHG"
     gene            complement(617..1666)
                     /gene="rlmN"
                     /locus_tag="PLR47_10115"
     CDS             complement(617..1666)
                     /gene="rlmN"
                     /locus_tag="PLR47_10115"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOG13346.1"
                     /translation="MAEKIHNPLPNLSDFTLQELEDFIAASGKEKYRARQIMKELHQS
                     GCLSFDAMTTLARDFRQTLAGAARIYRPSIVKIQKSQDGTQKVLLGLEDGLFVESVLI
                     PGKNHWTLCISTQAGCAMGCAFCLTARQGFKRNLTPAEIVGQVVTLKKETPEGAAVGN
                     IVMMGMGEPLANYENTVKAIRILVSDFGLGFSSRRITVSTCGLAPQILQLGRDICINL
                     AVSLNAHDDETRSRLMPVNKKYPLAALLDACKQYPMPGRRMLTYEYILIDGVNASRPD
                     AEKLARLLKDQRCKINLIVFNEYPGSPFKAPSAKDVSSFQQVLLDRHFTAMLRKSKGA
                     DILAACGQLSGSHSI"
     gene            complement(1659..>2289)
                     /locus_tag="PLR47_10120"
     CDS             complement(1659..>2289)
                     /locus_tag="PLR47_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOG13347.1"
                     /translation="YALSEKIDVWFSTKDTISKKYDAGFRDVFDREFAEHWKDRFAKA
                     GIEYFFTLIDDAVARIMKTEGGMLWALKNYDGDVMSDMIASASGSLAMMTSVLVSPFG
                     WYEYEAAHGTVQKHYYKHLKGEETSSNSMALIFAWTGGLRKRGEMDGTPEVVEFANKV
                     EAAALETVESGVMTGDLLLLAEKRPTNRKVNTEGFIDAIAGNLKKKIDG"
BASE COUNT          436 a          654 c          703 g          496 t
ORIGIN      
        1 gccggccggc cggtgcgcgt ggatgtgcgg ctggtttcgg cgacgaacac caatctggaa
       61 accgcctgcc aggagggaaa attccgaatg gacctgatgt accgcctcaa gtccgcctgc
      121 gtctgcctgc cgccgcttcg ggagagaaga ggggacattc ctcttctggc ttcccatttc
      181 ctgaaaaagg cctgcgcgcg ccatcagaaa aacattaccg ggttttctcc ggaagcgatg
      241 cagcggctga cgcacgggga ctatcccgga aatatccgcg agctttccca gatggtggaa
      301 aacgccgcgc tgctggccga aggccccgtg atcctgcccg cggatctggg cggaaacggc
      361 gtcgttttgg agccgcaggg ggacgccttc tcccgcaggt tgtgcacgct caaggaaaat
      421 gattcggcgc acgtgcgctt cgtgatggac gccaccggcg gcaaccgccg cgaggccgct
      481 caaattctgg gaatcacgct ccggcagctc cagagaaaac tggcggacat gaaggaatct
      541 tttcggaatc atggttgacg ggcatgtgcc ggtttctccg gtaaagcccc gtgaatgaac
      601 atttcaaagc attacgtcaa attgagtgag aaccgctgag ctgaccgcag gcggccagaa
      661 tgtcggcgcc cttgctcttg cgcagcatgg cggtgaagtg gcggtcgagc aggacctgct
      721 gaaaggacga cacgtctttg gcggacggcg ctttgaacgg tgagccgggg tattcattaa
      781 acacgatcag attgatcttg cagcgctgat ctttcagcag ccgggcgagc ttttccgcgt
      841 cgggccgtga ggcgttcacg ccgtcaatga gaatatattc atacgtgagc attcggcggc
      901 ccggcatggg gtattgcttg caggcgtcca gaagcgcggc gagcggatat tttttgttga
      961 ccggcatgag ccggctgcgg gtttcgtcgt cgtgggcgtt gagcgatacg gccaggttga
     1021 tgcagatgtc gcggcccaac tggaggatct gcggggcgag cccgcaggtg gagacggtga
     1081 ttcgacgcga ggaaaagccc aggccgaagt cgcttacgag aatccggatc gccttcaccg
     1141 tattttcgta gttggcgagc ggttcgccca tgcccatcat gacgatattg cccacggccg
     1201 ctccctcggg cgtttctttt tttagcgtaa ccacctgacc gacgatttcc gccggcgtca
     1261 gattgcgctt gaagccctgg cgcgccgtca ggcaaaaggc gcatcccatc gcacagccgg
     1321 cctgcgtgga aatgcaaagc gtccagtggt ttttccccgg aatgagcacg ctttcgacaa
     1381 aaaggccgtc ttcgaggccc agcagaactt tttgcgtgcc gtcctgcgat ttctggattt
     1441 tgacgatcga cggccggtag attctggccg cgccggcgag cgtctgacgg aaatcgcgcg
     1501 cgagcgtcgt catggcgtca aaggagaggc agccggactg atggagctct ttcatgatct
     1561 ggcgggcgcg gtatttttcc ttgccggagg cggcaatgaa atcttccagc tcctgaaggg
     1621 tgaagtccga cagattgggc aaaggattgt gaattttctc agccatcgat ttttttcttc
     1681 aggtttccgg cgatggcgtc gatgaatcct tccgtgttga ccttgcggtt ggtgggccgc
     1741 ttttccgcca gcagaagcaa atcgcccgtc atcacgccgc tttccactgt ttccagggcc
     1801 gccgcttcca ctttgttcgc gaattcgacg acctccggcg tgccgtccat ctcgccgcgt
     1861 ttgcgcaggc cgccggtcca ggcgaaaatc agcgccatcg agttggagga ggtttcctcg
     1921 cccttcaggt gcttgtagta gtgcttctgc actgtgccgt gcgcggcttc gtattcgtac
     1981 cagccgaagg gcgacaccag aacggaggtc atcatggcca gcgacccgct cgcggacgcg
     2041 atcatgtccg acatcacgtc gccgtcgtag tttttgagcg cccagagcat tccgccctcc
     2101 gtcttcatga tgcgcgccac cgcgtcgtcg atgagcgtaa agaaatattc gatgccggcc
     2161 ttagcaaacc tgtccttcca atgctcggca aattcgcggt caaagacgtc gcggaagccc
     2221 gcgtcgtact ttttggaaat cgtgtccttg gtggaaaacc acacgtcgat cttttcggaa
     2281 agcgcgtag
//
LOCUS       DAOSIM010000297         2288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_40632_length_2288_cov_5.960143, whole genome shotgun sequence.
ACCESSION   DAOSIM010000297 DAOSIM010000000
VERSION     DAOSIM010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40632_length_2288_cov_5.960143"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PLR47_10125"
     CDS             complement(<1..486)
                     /locus_tag="PLR47_10125"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG13348.1"
                     /translation="MGNLLVNTRDQQFLLFEQFGIEKVFDSEAYGGFSKDDVLMILSE
                     AEKMAVNDVFPTLKEGDEEGCHFKDGKVTVPKCFREPFKKYSEAGWINPMDSPDVGGQ
                     GLPHTAGYAVSELFCAANYAFMMYPGLTHGAANLINVYGNEQQKKKYMEKCFSGEWTG
                     TM"
     gene            complement(758..>1646)
                     /locus_tag="PLR47_10130"
     CDS             complement(758..>1646)
                     /locus_tag="PLR47_10130"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13349.1"
                     /translation="FAKSISYGNALTLDSTDFLDYLARDGETGIITMYLEGVKDGRLL
                     LEQVKKINRRKPVIIYKSGLTESGARAVSSHTGSLAGGEKIWKAFFRQTGAVRVESLE
                     EMADVAQAFHRLGKVRGKRVAVLGFGGGNGVSVADDCAKAGLELPVLSQNLIRKLRKR
                     IPPAGSMIRNPIDAAIAFVHLPLMGEVIDVVAKSGEMDNFIVSVPLDWLYNQAPDGTY
                     IETLARYLATEAGKCAGGKPMLVAWRQYEASPKIRRWIPVFKDILMKAGIPVYEGLPK
                     AVRALSNLSEYYEFQGLIK"
     assembly_gap    1647..1656
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1657..>2288)
                     /locus_tag="PLR47_10135"
     CDS             complement(<1657..>2288)
                     /locus_tag="PLR47_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOG13350.1"
                     /translation="TPKGNVVAVDALVILDEKPAALPTREKGKRKRQAEIRAALDVMA
                     NAQSVAVVGATRPDASGFAGMFGCMRNFGYPGRLYPVNPRLQDIDGIKAYPNLSSLPE
                     VADLVILSIPAPRVADVLKECAATGHKNIHIFSSGFKETGEEEGIRLQKEIEAVATAG
                     GLRVIGPNCMGFYVPKNRMLTWEDASTKVGPVSLISQSGGNAQDFSNYLT"
BASE COUNT          476 a          652 c          596 g          554 t
ORIGIN      
        1 catcgtgccg gtccattcgc cggagaagca tttttccatg tattttttct tctgctgttc
       61 attgccgtaa acattgatca ggttggcggc gccgtgggtc aggccgggat acatcatgaa
      121 agcatagttg gccgcgcaga acaactctga aaccgcgtag cccgcggtgt ggggcaggcc
      181 ctggccgccg acatcgggag aatccatcgg gttgatccac ccggcttcgc tgtatttttt
      241 aaacggttcc cggaaacact tgggcacggt gactttgccg tctttgaaat gacagccttc
      301 ttcgtctcct tcttttaaag tcggaaaaac gtcattgacg gccatttttt cggcttcgct
      361 caagatcatc aatacgtcat ccttggaaaa acctccatag gcctcggaat caaaaacttt
      421 ttcaatcccg aactgctcaa aaagaagaaa ctgctgatcg cgtgtgttga ctaataaatt
      481 gcccataact gactcccttc gtaaaagtgg tttatttttt taacgttcat agacgtccgt
      541 tttgtgaaga ataacttcgg aatttttcat gatcagtgca gcagaataca aatcgatttt
      601 gaaacatccc gcctgaagct ttctggccgt ggttccattt caggaagttg actataagta
      661 caaacgtgac cgaaagtcaa tcaaaatgat gaaaaaatga ggcgaatcgg cggccgtttt
      721 ttgccccggg ggtcattccg gtcgacgcct ggtggattta ttttataagt ccttgaaatt
      781 cataatattc tgacagattt gataaggccc ggaccgcctt gggcagccct tcgtaaaccg
      841 ggattccggc tttcatgaga atgtctttga aaaccggaat ccatcgccgg attttcggcg
      901 aagcctcata ctgacgccag gccaccagca tgggtttgcc gccggcgcat ttcccggctt
      961 ccgtcgccag atacctggca agcgtttcga tatacgttcc gtccggcgcc tggttgtaaa
     1021 gccagtccag cggaacggag acgatgaaat tgtccatctc tccacttttc gcgacaacat
     1081 caatcacttc acccatcagg ggcagatgaa caaaggcaat cgccgcgtca atgggattgc
     1141 ggatcatcga cccggccggc ggtatgcgtt tccgaagctt ccggatcagg ttttgggaaa
     1201 gcacgggaag ctccagaccc gctttcgcgc agtcatccgc caccgacacg ccgttgcctc
     1261 cgccgaatcc gagaacggcc accctttttc ccctgacctt ccccagccgg tggaacgcct
     1321 gcgcgacatc agccatttct tcgagcgatt ccacacggac cgctcccgtc tggcgaaaga
     1381 aagccttcca tatcttttcc ccgccggcga gcgatcccgt atgcgaagac accgccctcg
     1441 ctcccgattc cgtgaggccg cttttgtaga tgatgacggg ctttcggcga ttgattttct
     1501 tcacctgctc aagcaggagc cggccgtcct tcacgccctc gagatacatg gtgatgattc
     1561 cggtttcccc gtcgcgggcc aggtaatcca ggaaatccgt gctgtcgagc gtcagggcgt
     1621 tgccgtagct gatggacttg gcaaagnnnn nnnnnncgtc aggtaattgg aaaaatcctg
     1681 ggcgttgccg ccgctctggc tgatcaggga gacggggccg acctttgtgg aggcgtcttc
     1741 ccaggtcagc attcggtttt tgggaacgta gaagcccatg cagttcggtc cgatgacgcg
     1801 aaggcctcct gccgttgcga cggcttcaat ctccttctgc agccggatgc cctcttcctc
     1861 gccggtttcc ttgaagccgg agctgaaaat atggatattt ttgtgccccg tggcggcgca
     1921 ttccttcagc acgtccgcga ccctgggggc gggaatcgag agaatcacca ggtccgcgac
     1981 ttccggcagc gacgacaggt tcgggtaggc tttgatcccg tcgatgtcct gaagccgcgg
     2041 gttcacggga tagagtcttc ccgggtagcc gaaatttctc atgcagccga acatcccggc
     2101 aaatccgctt gcgtccggac gggtggcgcc gaccacggca acggattggg cgtttgccat
     2161 cacgtcgagc gcggcgcgga tttccgcctg tctctttcgc tttccctttt cacgagtcgg
     2221 caaagcagcg ggcttttcat ccagaatcac cagcgcatcc accgccacga cattcccctt
     2281 cggagttg
//
LOCUS       DAOSIM010000298         2273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_40985_length_2273_cov_7.454013, whole genome shotgun sequence.
ACCESSION   DAOSIM010000298 DAOSIM010000000
VERSION     DAOSIM010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40985_length_2273_cov_7.454013"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..999
                     /locus_tag="PLR47_10140"
     CDS             250..999
                     /locus_tag="PLR47_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG13351.1"
                     /translation="MTKKDSKLFLHLKKWLEKAVLDYGMIEEGDRVLVGVSGGADSFA
                     LLDLLGSPMIFVPQFSWTAVYIDMGFDPGFVAYRLLEEYFQTHQHPYVMEKTDIGTIS
                     HSDFNRKNPCFLCSRLRRKRIFEIADEKGCNKIAFAHHKDDIIETLLINLFYGREIST
                     MMPNQPIFDGKLHIIRPLAYLSEALVKKYSRERQFPAVKNACPTSGNSRRSYIKKLLD
                     QLEIDNPDIRHNIYTAMSHVKPDYLMPGIKK"
     gene            1103..1579
                     /gene="smpB"
                     /locus_tag="PLR47_10145"
     CDS             1103..1579
                     /gene="smpB"
                     /locus_tag="PLR47_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOG13352.1"
                     /translation="MSKDNAAHKLIASNKFARVNYDIDDVYEAGMVLSGTEVKALRAG
                     KANLKDSYAVVKDSEVYLRDMHISHYDFGNRANHEPLRPRKLLLHKREIRKLYGKTRE
                     KGLALVPLRLYFKNGMVKVEIGVGKGKKMHDRREDIKQREERRSIARDFKSKRIRV"
     gene            1659..2147
                     /locus_tag="PLR47_10150"
     CDS             1659..2147
                     /locus_tag="PLR47_10150"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOG13353.1"
                     /translation="MKGKKVSESRVMMGQGMNPENANPAGNVHGGDIMKLIDTAGGIA
                     ATRHARAHVVTASIDRMDFHHPVYIGDFLILKASVNFVGKTSMEVGVRVEAENPISGV
                     KRHTGSAYLTFVALDANKKPMQLPPLILETEEERLRNAEASERRQMRLAEKKKEKKKH
                     EK"
BASE COUNT          610 a          541 c          641 g          481 t
ORIGIN      
        1 cggcaggagc ggcggcaggt gcaggcgcag cggcgggcat cggcgcggga acgattgccg
       61 cagcggcagt ggttgcagcg gcggcggtcg cggcagtggt tgcgacgacg gggactacgg
      121 attctactcc cacgcataca acgccggcgc acccataaga ataaatctta ttgaatggat
      181 tcagtccccc tttcaccgga cgttcatcgc cggtgaaagg ggattttttt atacccggaa
      241 agcggcggaa tgaccaaaaa agattctaaa cttttcctgc atttgaaaaa atggctggaa
      301 aaagcggttc tggactacgg catgattgaa gaaggagacc gggttctggt cggtgtttcc
      361 ggcggggcgg acagcttcgc ccttttggat ctgctcggct cgccgatgat ttttgttccg
      421 caattttcct ggaccgccgt atacattgac atgggctttg atccgggctt tgtggcgtat
      481 cgattgctgg aagaatattt ccagacgcac caacatccgt atgtaatgga aaaaacagac
      541 attgggacga tttctcattc tgatttcaac cggaaaaatc cgtgctttct gtgttcccgt
      601 ctgcgccgga aaagaatttt tgaaattgcg gatgaaaagg gctgcaataa aatcgcgttt
      661 gcccaccaca aggacgatat cattgaaacg cttctcatca acctgtttta cggccgggaa
      721 atcagcacca tgatgccgaa tcagccgatt ttcgacggca agcttcatat tatccgtccg
      781 ctggcctatt tgagcgaagc gctggtaaaa aagtacagcc gtgagcggca gttcccggcg
      841 gttaaaaacg cctgcccgac gagcggaaat tcgcgccgaa gctatatcaa gaaattgctg
      901 gatcagctcg aaattgacaa tccggacatc cgtcacaata tttatacggc tatgagccat
      961 gtgaagccgg actacctcat gccgggaata aaaaaataat caacgctctt tccctccttt
     1021 tgcagccgcc tgaaggcggt gcgaggagga cagagtgtgt tttaacagtg gcaataaaca
     1081 aaatttaatg tagaatcaaa tcatgtcaaa ggataacgcg gcccataaac ttatcgcgtc
     1141 caacaagttc gcaagagtca attacgacat tgacgacgtc tatgaggcgg gtatggtcct
     1201 gtccggcact gaagtgaagg ccctgcgcgc cgggaaggcc aatctcaaag acagctatgc
     1261 ggttgtcaaa gacagcgaag tctatctgcg tgatatgcat atcagccact acgatttcgg
     1321 caaccgggcc aaccatgaac ctttgcggcc gcgcaagctg cttttgcaca agcgcgagat
     1381 ccgcaaattg tacggaaaaa cccgcgaaaa gggattggcg ctggtgccgc tgcggctgta
     1441 tttcaaaaac ggcatggtca aggtggaaat cggcgtcggc aagggaaaga aaatgcacga
     1501 ccgccgcgag gacatcaagc agcgggaaga acgccgatcc atcgcccggg atttcaaatc
     1561 caagcggatt agagtttaat ttttttatct cttgccaatg ccgcggcggc gggtaaggaa
     1621 acgagctgcg aatgacgaga tgcctgaggc gaggttttat gaaaggcaag aaagtcagcg
     1681 aaagcagagt gatgatgggg caggggatga acccggaaaa cgccaatcct gccggcaatg
     1741 tccatggcgg agacatcatg aaactgattg acacagccgg cggcatcgcg gcaacacgcc
     1801 atgcaagggc gcatgtcgtg acggcctcca ttgaccggat ggatttccat cacccggttt
     1861 acatcgggga ttttttaatc ctcaaagcct ccgtcaattt tgtcggcaag acatccatgg
     1921 aagtcggcgt ccgcgtggag gcggaaaatc ccatttcggg ggtaaaacgc cataccggct
     1981 ccgcctatct gacctttgtg gcgctggatg cgaacaaaaa gccgatgcag ctgccgccgt
     2041 tgattctgga aacggaagaa gagcggcttc gcaacgcgga agcgtccgag aggagacaaa
     2101 tgcggttggc ggaaaagaaa aaagagaaga aaaagcacga aaagtgagat tggcggttgc
     2161 cgccgctttt ctttgacaaa ccgccttttg aggattatag tataaacaaa taaaaaaagg
     2221 ggggcgtaac ggtttcgacg gggacatttg aagttgtaga agcgtgccga ggt
//
LOCUS       DAOSIM010000299         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_41008_length_2272_cov_5.668020, whole genome shotgun sequence.
ACCESSION   DAOSIM010000299 DAOSIM010000000
VERSION     DAOSIM010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41008_length_2272_cov_5.668020"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2272
                     /locus_tag="PLR47_10155"
     CDS             <1..>2272
                     /locus_tag="PLR47_10155"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOG13354.1"
                     /translation="RDPLGFSSVILERMSALPWAGKVQFYSGQLLSADGRNALIIARI
                     AGSGTDTAKAARIEKLLEDCRRELAAQKALKGAYILTPVGAYRAALDNETIARRDVAM
                     AVTLTTICIILLLFVAFPRPLIGLLSFLPSSVGAIAALFVCSFLFKSMSILSVGFGGA
                     IMGFTVDMGIAYLLFLDQPHATYGKQVAREVWSAEFMATLTTVGAFLLLLISDFKILS
                     EIGVFSALGVAFALLFVHFVFPRVFPSMPPAKRRTNRFLLNAIIKIAAPAKWKLAAAI
                     LFGLMMLIFAKPVFNVDLQAMNSVSKETISAEKKLQEIWGNLSGKCYVLLEASDMGEL
                     QKKNEQLMRQLAVDVRQGKLAPVFLPSVLFPSQPAAAGNFAAWQSFWNKDRVDALKRE
                     LDAAARGNGFAPDAFDPFWRMLQSDAPGVFQISPQYFEMLGITKTANGYTQLSRLSAG
                     ENFSAENLFNRISATGIVNLFDADLFNKRLSDFLKNMFLEIALIISIGLILITFLHFL
                     DWRLSLAALAPVAFALCATLGTLKLIGHPLDIPGIMLWIVVMGMGDDYALYYICHYQR
                     NFDEKRPAMHTIKFSMFLSACTTMIGFGVLMFAEHPLLKSIGIVSFLGIGYALIGAYF
                     ILPVLMEKIFAPFAFPSGAVEACSREHFRRTILRYRHLPGYPRVFARFKLMMDPMFRE
                     VHQYVKNPRRILDIGCGYGVPATWLLELHPEAKVYGLEPDEERVLIASRVIGARGRAQ
                     VGRAPDLPDVEGSVDHV"
BASE COUNT          436 a          612 c          628 g          596 t
ORIGIN      
        1 cccgggaccc gctgggtttt tcctcggtga tcctggagag aatgtccgcg ctgccctggg
       61 cgggcaaagt ccaattttat tcgggtcagc tcctttccgc ggacggcaga aacgccctga
      121 tcatcgcccg cattgccgga tcgggaacgg atacggcaaa ggccgcccgg attgaaaagc
      181 ttctggagga ttgccggcgg gagctcgccg cgcaaaaagc attgaagggc gcctatatcc
      241 ttacgccggt gggcgcttat cgcgcggcgc tggataacga aaccattgcc aggcgtgatg
      301 tggcaatggc cgtaacgtta acgacgatct gcatcattct tctgctcttt gtcgcttttc
      361 cccgtccgct gatcggtctt ttgtcatttc tgccttcatc ggtaggcgcc attgccgcct
      421 tgtttgtctg ctctttttta ttcaaatcca tgtccatact gtccgtcggc ttcggcggcg
      481 ccattatggg ctttaccgtt gatatgggaa ttgcatatct gcttttcctg gatcagcccc
      541 acgccactta cggcaaacag gtggcgcgcg aagtttggtc ggcagagttc atggcgacac
      601 tgaccactgt cggcgcattt ctgcttctcc tgatcagcga ctttaaaatt ctgtccgaaa
      661 tcggcgtctt ttccgcgctg ggtgttgcct ttgcgctgtt atttgtccat tttgttttcc
      721 ccagagtatt tccgtccatg ccgcccgcca aacgccggac caaccgtttc ctgttgaacg
      781 cgattatcaa aatcgccgcc cccgcaaagt ggaaattggc cgcagcgatt ctcttcggcc
      841 tgatgatgct gatttttgcc aagccggttt tcaatgtcga tttgcaggcc atgaattccg
      901 taagcaagga gaccatcagc gccgaaaaaa aattacagga aatctggggc aatctttccg
      961 gtaaatgtta cgttcttctg gaagcgtcgg acatgggaga attgcagaaa aaaaacgagc
     1021 aattgatgag gcaacttgcc gtcgatgtgc ggcagggaaa actcgcgccg gtattcctgc
     1081 cgtccgtttt gtttccgtct cagcccgcgg ctgccggcaa ttttgccgcc tggcaaagtt
     1141 tctggaacaa agatcgcgtg gacgcattga agcgtgaact tgatgcggcg gcgcggggaa
     1201 acggctttgc gccggatgcc tttgatccct tctggagaat gcttcaaagc gacgctccgg
     1261 gtgttttcca gatttccccg cagtattttg agatgctggg cattacaaaa acggcgaacg
     1321 gatatacaca actcagccgg ctctccgccg gggaaaattt tagcgcggaa aatttattta
     1381 acaggatttc cgccacgggg atcgtcaacc tgttcgacgc ggacttgttc aataaaaggc
     1441 tcagcgattt tttgaaaaat atgtttctgg aaatcgctct gatcatcagc atcgggctca
     1501 ttctcatcac ctttttgcat tttctggact ggcgtttgtc gcttgcggcg ctggcgccgg
     1561 ttgccttcgc gctttgcgcc acgctgggga cgctcaagtt gatcggccat ccgctggata
     1621 ttcccggcat catgctgtgg attgtcgtga tgggcatggg ggatgattac gctctttatt
     1681 atatctgcca ttatcagcgc aactttgatg aaaaacgtcc ggccatgcac accatcaagt
     1741 tttcgatgtt tctgtcggcc tgcaccacca tgatcggttt tggtgttctg atgtttgccg
     1801 agcacccgct tttgaagagc attgggatcg tctccttttt aggcatcggc tatgcgttaa
     1861 tcggggccta ttttattttg ccggtattga tggagaaaat ctttgctccg tttgcgtttc
     1921 cgtcgggggc cgtcgaagcc tgttcgagag agcactttcg ccggacgatc ctgcgctatc
     1981 gtcatctgcc cggctacccc agagtctttg cccgcttcaa gctgatgatg gacccgatgt
     2041 tccgggaagt gcaccagtac gtaaagaatc cccgccgcat tctcgatatc ggctgcggct
     2101 acggcgttcc cgcgacctgg ctgctggagc tacatccgga agccaaggtt tacggcctgg
     2161 agcccgatga agagagagtg ctcattgcca gccgcgtgat cggcgcgcgg ggccgggcgc
     2221 aggtcgggcg ggcgccggat ctgccggacg tcgaaggttc ggtggaccac gt
//
LOCUS       DAOSIM010000300         2254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_41439_length_2254_cov_7.651660, whole genome shotgun sequence.
ACCESSION   DAOSIM010000300 DAOSIM010000000
VERSION     DAOSIM010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41439_length_2254_cov_7.651660"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PLR47_10160"
     CDS             <1..305
                     /locus_tag="PLR47_10160"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG13355.1"
                     /translation="ADMKIGHDTLAYLVYRSGWLKDKGLPNVMETSMSKLYGAKVVVH
                     DANECMKLYGSFGYSDEYPCGRFLRDSKQFETLEGTSNMHTMIVANAVMGFAPNRA"
     gene            516..>2254
                     /locus_tag="PLR47_10165"
     CDS             516..>2254
                     /locus_tag="PLR47_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOG13356.1"
                     /translation="MKQYFEKMDPLGKPLSAKQVESMQENCAKIEEIMKTIDAEVERI
                     KNVGVSLQTLHDRGEMSVWERIEHLVDSGTFCPLHSIFDPENEESGSTGVVDGLARIG
                     GKWCALIGFNNKWIAGAWIAGQPDNNLRVTDIARILHIPLVWLVNCSGVKLPEQEKMY
                     ANRRGQGTCFFRHAELEQMGIPVIAGIYGTNPAGGGYQSISPTILLAHKKANMAVGGG
                     GIVSGMSPKGFFDEEGAEELINATRHFKSVPPGSVKVHHDVTGFFRAVFDEETQVLDA
                     IKDYMDDLPAYHPRFFRVAAPAEPAYPPEEIASIIPVNKKRVYDFEQVLARLVDGSEH
                     LEFRPGFGPEMYCGLVKVDGYLMGVIGNRQGAFPNYPEYTNEYMGVGGKLYRQGLIKM
                     SEFVTLCSRDKVPMVWFQDTSGIDVGDIAEKAELLGLGQSLIYSIENSKLPMMLVVLR
                     KGTAAAHYVLGGPTANNNNAFSLGTATTEINVMHGETAAAASYARRLVKEKDAGRPLG
                     PIIDKMNEMVKHYDDTSGPMFCAKKGFVDEIVRYHELRNYLVGFANCCYQNPRSICPQ
                     HQMILPRIIRSQIV"
BASE COUNT          512 a          642 c          672 g          428 t
ORIGIN      
        1 tggcggacat gaaaatcggc catgacacgc tggcctatct ggtttaccgc tcgggctggc
       61 tcaaggacaa gggacttccc aacgtcatgg aaacctccat gtccaagctt tacggcgcaa
      121 aagtcgtcgt ccacgacgcc aatgagtgca tgaagctcta cggttcgttc ggctattccg
      181 acgaatatcc ctgcggccgc ttcctgcgcg actccaaaca gttcgagacg ctggaaggca
      241 cgtccaacat gcacacgatg atcgtcgcca acgccgtcat gggatttgcg cccaaccggg
      301 cataacgaga aatatgccct gatgccctct ccctcgacgg gagagggcat cagggtgagg
      361 gtgaaaaatc ttgatgtcat tcgaaagatt ttttcctccg gtcttgcccc atcaatgggg
      421 ctggaaatga caaacgttta atcaaacatt gcatgtgtac cccctcccct tcatcccctc
      481 ccacaagggg aggggatgtt tataaggaga ccgccatgaa acaatacttt gagaaaatgg
      541 atccgctggg gaaacccctt tccgccaaac aggtcgaaag catgcaggag aactgcgcga
      601 agatcgaaga gatcatgaaa accatcgacg cggaagtcga gcggatcaaa aacgtgggcg
      661 tctctttgca gacgctgcac gaccggggcg agatgagcgt ctgggaacgc atcgagcacc
      721 tcgtcgattc agggaccttc tgcccgctgc acagcatctt cgatccggaa aacgaggaat
      781 cgggaagcac cggcgtcgtg gacggtctgg cgcgcattgg cggcaaatgg tgcgcgctca
      841 tcggatttaa caacaagtgg atcgcgggcg cgtggattgc cggccagccg gacaacaacc
      901 tgcgcgtgac cgacatcgcc aggattctgc acattccgct ggtgtggctg gtcaactgct
      961 ccggcgtgaa gctgccggaa caggaaaaaa tgtacgccaa tcgccggggg cagggcacct
     1021 gctttttccg gcatgcggag ctcgaacaga tgggcattcc ggtcatcgcc ggcatttacg
     1081 gcaccaatcc ggcgggcggc ggctatcagt cgatcagccc gacgattctg ctggcgcaca
     1141 agaaggccaa catggccgtg ggcggcggcg gaatcgtgag cggcatgtcc cccaaagggt
     1201 ttttcgatga agaaggagcg gaagagctta tcaacgccac gcggcacttc aaatccgttc
     1261 cgcccgggag cgtgaaagtc caccacgacg tcaccggatt tttccgggcg gtctttgacg
     1321 aggaaacaca ggtcctcgac gccatcaaag actatatgga cgatctgccc gcctaccatc
     1381 cgcgcttctt ccgcgtggcg gctcccgccg aacccgctta cccgcctgag gagatcgcct
     1441 cgatcattcc ggtgaacaag aagcgcgttt acgatttcga gcaggtgctg gcgaggctgg
     1501 tggacggctc agagcacctg gagttccgcc ccggcttcgg accggaaatg tactgcggcc
     1561 tggtcaaggt ggacggttat ctgatgggtg tgatcggcaa ccgtcagggc gccttcccca
     1621 attatcccga atacaccaac gagtacatgg gcgtgggcgg caaactctac cgccaaggcc
     1681 tgatcaagat gagcgagttc gtgacgctgt gctcgcgcga caaagtgccg atggtatggt
     1741 ttcaggacac gtccggcatc gacgtgggcg acatcgccga aaaggcggag ctgctgggtc
     1801 tgggacagtc gctgatttat tcgattgaga attcaaaact gccgatgatg ctggtcgtcc
     1861 tgcgcaaggg gacggcagcc gcgcattatg ttctgggagg ccccacggcc aacaacaaca
     1921 atgcattttc gctgggcacc gccacgaccg aaatcaacgt catgcacggg gaaaccgcgg
     1981 cggcggcaag ttacgcgcgg aggctggtga aggagaagga cgcgggccgg ccgctggggc
     2041 ccattatcga caagatgaat gagatggtga agcactatga cgacacgtcg gggccgatgt
     2101 tttgcgcgaa gaagggcttt gtcgatgaaa tcgtccgcta tcatgaattg cgcaactacc
     2161 tggtgggctt tgccaactgc tgctaccaga atccccggtc catctgtccg cagcaccaga
     2221 tgatccttcc ccggatcatc cgctcgcaga tcgt
//
LOCUS       DAOSIM010000301         2251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_41522_length_2251_cov_5.633880, whole genome shotgun sequence.
ACCESSION   DAOSIM010000301 DAOSIM010000000
VERSION     DAOSIM010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41522_length_2251_cov_5.633880"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..>767)
                     /gene="truA"
                     /locus_tag="PLR47_10170"
     CDS             complement(2..>767)
                     /gene="truA"
                     /locus_tag="PLR47_10170"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOG13357.1"
                     /translation="KIVLEYDGTNYSGWQAQKNARSIQETLIAAARKFLGVPVELQGA
                     GRTDAGVHALGQVAHLECARKVNGDALRMGINDLLPAGINILSAQSAHPRFHARHSAV
                     ARSYLYLIATRRTAFAKKYVWWVKDSLHIGKMRRALQVFEGFHDFSSFADKRMEKSAS
                     TTVHLESARLEEFGQIIAMRLVGSHFLWKMARRIVGMTVEAGRGQVSAEDLAGMLKNF
                     SDAPARHTAPPSGLFLEKVLYEGDRLPDIQPPVHLL"
     assembly_gap    768..777
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<778..2119)
                     /locus_tag="PLR47_10175"
     CDS             complement(<778..2119)
                     /locus_tag="PLR47_10175"
                     /inference="COORDINATES: protein motif:HMM:NF024314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family N-terminal domain-containing
                     protein"
                     /protein_id="HOG13358.1"
                     /translation="MNRMCGLAVIFFFILSGCVSAPETVQDVRDLRQDHAAYLTDIAG
                     GGDPLPAALQARLDEDYNTIYFSVWHQSRPFHAWEERVRRDFARYGLKPGYGENKRLH
                     PSSWLKKMQHNAALDDYPNALTRGITTRNTNLRELPTNSPHFTSANGDSSAWPFDNLQ
                     RSSVPAGTPVFISHVSADKAWALVETSFTYGWMPVEDFARVNEDFVKTWESGRYAVIL
                     RDSTSVLDRNGRFLVKASVGHLFPFTATQDGKMQISVAAADQNHQAVIRQGVVPAEAA
                     AAKPLRFTPANAARIANEMIGEPYGWGGLYGNRDCSAMTRDFFTVFGIWLPRHSEDQV
                     KEAGATIDLRGLSPEEKERIIMEKGVPYLSLLWRKGHVMLYIGASKGRALIFHNIWGI
                     RIKDLQGHEGRKIIGQAVITGLTPGRELSDMDTAAGSLLDNIAAMNILSADVREA"
BASE COUNT          428 a          651 c          672 g          490 t
ORIGIN      
        1 tttacagcag atgaaccggc ggctgaatat ccggcagccg gtcgccttcg tatagaactt
       61 tttccagaaa aagcccggag ggaggggccg tgtggcgggc gggagcatcg gaaaagtttt
      121 tcagcattcc ggccaggtct tcggccgaaa cctggccgcg cccggcctca acggtcatgc
      181 ccacgatgcg ccgcgccatt ttccacagaa agtgagagcc gaccaggcgc atcgcgataa
      241 tttgtccgaa ttcctcaagc cgcgcggatt ccagatgcac ggtggtggaa gcgctttttt
      301 ccattcgttt gtcggcaaag gaggaaaagt cgtgaaagcc ctcaaaaacc tgaagcgccc
      361 gccgcatttt gccgatgtga aggctgtctt ttacccacca gacatatttt ttggcaaagg
      421 cggtgcgtct cgtcgcgatc agatacaggt agctgcgggc cacggccgag tggcgggcgt
      481 gaaaccgcgg gtgggcgctt tgtgcgctca ggatgttgat gcccgccggc agcaaatcat
      541 tgattcccat ccgcagggca tcgccgttca ccttgcgggc gcattccaga tgggcgacct
      601 ggcccagcgc gtggacgccg gcgtcggtgc gccccgctcc ctgcaattcc accgggacgc
      661 caaggaattt ccgggccgcg gcgatcagcg tttcctggat gctcctggcg tttttctgcg
      721 cctgccagcc gctgtagttg gtgccgtcat actcgagaac gattttgnnn nnnnnnngcg
      781 cctcccggac gtccgcggac aggatgttca tggcggcgat gttgtccagc agagaaccgg
      841 ccgccgtgtc catatccgaa agctcccgtc ccggcgtcag accggtgatt accgcctgtc
      901 cgatgatttt tcgcccttca tgcccctgca gatcttttat ccggatgccc cagatattgt
      961 ggaagatgag cgcccggcct ttcgaagcgc cgatgtacag catcacgtga cccttgcgcc
     1021 acagcaggct caggtacggg acgccttttt ccatgatgat tctttccttc tcttcgggcg
     1081 acaggccccg caggtcgatg gtagcgcccg cttccttcac ctggtcttcc gagtgccgcg
     1141 gcagccagat gccgaagacg gtgaaaaaat cacgggtcat ggccgagcag tcgcggttgc
     1201 cgtaaagacc gccccagccg taaggttcgc cgatcatttc gttggcgatc cgggccgcat
     1261 tggccggggt gaagcgaagc ggttttgccg ccgctgcttc cgcggggaca accccctgcc
     1321 ggatcaccgc ctgatggttc tgatccgctg ccgcgacgga gatttgcatc tttccgtcct
     1381 gcgttgcggt gaaagggaaa agatggccca cggacgcctt tacgaggaag cgaccgttgc
     1441 ggtccagaac agacgtggaa tcgcgcagaa tgaccgcgta gcgtcccgat tcccaggttt
     1501 tcacgaaatc ttcgttgacc cgggcaaagt cttccaccgg catccagccg taagtaaagg
     1561 aggtttccac cagtgcccag gctttgtccg cgctgacgtg ggagatgaaa accggcgtgc
     1621 cggccggcac ggacgaccgc tgcagattgt cgaaaggcca ggcggagctg tctccgtttg
     1681 cgctcgtgaa gtggggggaa ttcgtgggca gctcccgcaa attcgtgttc cgggtcgtga
     1741 tcccgcgggt gagcgcgttg gggtagtcat ccagggcggc attgtgctgc atttttttca
     1801 gccacgagga gggatgcagc ctcttgtttt cgccgtagcc gggtttgagg ccgtatcttg
     1861 caaaatcgcg gcggactctt tcctcccagg cgtgaaacgg acggctctga tgccagacgg
     1921 aaaaataaat cgtgttgtag tcctcgtcca gccgggcctg cagggctgcg ggcagcgggt
     1981 cgccgccgcc ggcgatgtcc gtcagatagg cggcgtggtc ctggcgaaga tcccggacat
     2041 cctgaacggt ttcgggcgcc gatacgcaac cggataaaat aaaaaagaaa atcactgcca
     2101 gcccgcacat tctgttcata aaatcaccct tcatggaaga tgctgtttcc tataatcttt
     2161 ttttgcgttt cgatcaagat aaacgggacc gggcattccc atcattttct tttgcattct
     2221 tgaaaatttt gtgtaaatta cggccatgat t
//
LOCUS       DAOSIM010000302         2246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_41657_length_2246_cov_5.448197, whole genome shotgun sequence.
ACCESSION   DAOSIM010000302 DAOSIM010000000
VERSION     DAOSIM010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41657_length_2246_cov_5.448197"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PLR47_10180"
     CDS             <1..325
                     /locus_tag="PLR47_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13359.1"
                     /translation="IACPLCREKYGGADGRHQETEGAILARAFLEGSGCSAEFVERVI
                     FLVGHHHTLTGIDGLDYQILIEADYLVNADEGKCSQANIRNTMARVFKTDTGISLLKS
                     MYRLD"
     gene            322..999
                     /locus_tag="PLR47_10185"
     CDS             322..999
                     /locus_tag="PLR47_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13360.1"
                     /translation="MNPAPGPLRQALLFDIVGKDKREFVAQIIYILWGDKMPTLKKIR
                     DTMKKQKINGEIMEQMDFEADCNNPHNIISLIDKMDELLTKEQCLSIMEKQGCCKGGQ
                     RDKDCKAFALGHTDKPLSEKITLMSSIQYMMSPCLNDDGTITVTFDGYQNGVHTGKNT
                     CSCSSIKKLKQPFSISPTYCGCCAGHFLYHYQNALGVKLKLKEINSSPLNTNGENPCK
                     FTFEVLD"
     gene            1126..1455
                     /locus_tag="PLR47_10190"
     CDS             1126..1455
                     /locus_tag="PLR47_10190"
                     /inference="COORDINATES: protein motif:HMM:NF007495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HOG13361.1"
                     /translation="MIAIEDFEKVDMRVGTVISASINEKARNPAYKLAIDFGGEIGVK
                     TSSAQLTALYTADRLIGMQVVAVINFPPRQVAGVRSEVLVLGCSAKQGTVLLHPAETV
                     GNGDRIY"
     gene            1560..2126
                     /locus_tag="PLR47_10195"
     CDS             1560..2126
                     /locus_tag="PLR47_10195"
                     /inference="COORDINATES: protein motif:HMM:NF024423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG13362.1"
                     /translation="MDVRAYIQLMNTIEALKCNTRHSWTSSGRRESVAEHSWRLALMA
                     YFVKDEFPEADIDKVILMCLCHDLGEAFTGDIPAFEKTAADEIAEQNAIGAFTARLAE
                     PYGSGLTRLFAEMAEMKTLEARIYKALDKTEAILQHNEADIATWLPLEYHENLVYGGD
                     EVAFSKYLSDLKAELNRDSIRKIEAAEK"
BASE COUNT          625 a          516 c          599 g          506 t
ORIGIN      
        1 catcgcctgc ccgctgtgcc gtgaaaaata cggcggcgcc gacgggcggc accaggaaac
       61 ggaaggcgcg attttagccc gggcgttcct cgagggctcc ggctgttccg ccgaattcgt
      121 cgagcgcgta atatttctgg tcgggcatca tcatacgctt acgggcatcg acggattgga
      181 ttatcagatt ttgattgaag ccgactatct tgtaaacgcg gatgaaggca agtgttcgca
      241 ggcgaatatc cgcaatacga tggcgcgcgt atttaagacg gatacgggta tttccctgct
      301 caaatccatg tatcggctcg attgaatccc gcgccggggc cgttgcggca ggcgcttctt
      361 tttgatatag ttggaaagga taaacgggaa tttgtagcgc agattattta tattttgtgg
      421 ggggataaaa tgccgacttt aaaaaagata agagacacaa tgaaaaagca gaaaatcaac
      481 ggcgagatta tggaacaaat ggactttgaa gctgactgca ataatccgca taatattatt
      541 tctctcattg ataaaatgga tgagttgctt acaaaggagc aatgtctttc aatcatggaa
      601 aaacaaggtt gctgcaaagg aggtcagcgt gacaaggact gtaaggcgtt tgccttggga
      661 cacacggata agcctctttc tgaaaaaatt acattaatgt caagtattca atatatgatg
      721 tcgccttgtc tgaatgacga tggtacaatt acggttacat ttgacggcta tcaaaatggt
      781 gttcacacag gtaaaaacac ttgttcctgt agttcgatta aaaaattaaa acagcctttt
      841 tccatttcgc ctacatactg cggttgttgt gcaggacact ttttatatca ctatcaaaac
      901 gctttgggag taaagctgaa attgaaagaa atcaactcat caccacttaa tacaaacggt
      961 gaaaacccgt gcaaatttac atttgaagtt ctcgattaat atgacattat taagcataat
     1021 gcgaagcggc taaattccaa tttggtactc ttaaagcgta ggtgatggcg gatcgcttgc
     1081 gctttaggag taccaaacag gagtttgacg gaggaaacca aagccatgat tgccatagaa
     1141 gactttgaaa aagtggatat gagggtcggt acggtcatat cggcctccat aaacgaaaaa
     1201 gccaggaacc ccgcttataa actcgccatc gactttggcg gcgaaatcgg ggtcaaaacg
     1261 tcttccgcgc agcttacggc gctttacacg gccgaccggc tgattggcat gcaggtcgtt
     1321 gcggtgataa acttcccgcc ccgccaggtg gccggcgtca ggagcgaggt gctggttttg
     1381 ggatgcagcg caaaacaggg aacggtattg ctccatccgg cggaaaccgt tggaaacggc
     1441 gatcgtattt attaaacgcc catgcgataa atcccatttt gccggacggc ttcaaaccgc
     1501 ggattcgcct gtaacagatt gaaaaaagac aaacaaagcg tttcggggag gaattcgcca
     1561 tggatgtccg cgcttacatt caattaatga acaccataga ggcgctcaaa tgcaacacga
     1621 ggcattcgtg gacctccagc gggcggcgcg agagcgtcgc ggagcattcg tggcggcttg
     1681 ccctcatggc ttatttcgtg aaggacgagt tccccgaagc cgacatcgac aaggtcatcc
     1741 tgatgtgcct gtgccacgac ctgggcgaag ccttcacggg cgacatcccg gcgtttgaaa
     1801 aaaccgccgc cgacgagatc gcggagcaaa acgcgatcgg cgcattcacg gcccggctgg
     1861 cggaaccgta tgggagcggc ctgaccaggc ttttcgccga aatggcggag atgaaaacat
     1921 tggaggcgcg gatctacaag gccttggata aaacggaagc catccttcag cacaacgagg
     1981 cggatatcgc aacgtggctg cccctcgaat accatgaaaa cctcgtatat ggcggggatg
     2041 aagtggcctt ctcaaaatac ctgagcgatc tgaaagcgga gctcaacaga gattccatcc
     2101 gaaagatcga agccgcggaa aaatagcgtt cggcttctcc tgccggcgcg ccggggccgg
     2161 gattccatcc gatttagaag aagaacaaat cgcggctcta cactaaagat tggggtgaag
     2221 ggataaaaag gggaagcata ccgctt
//
LOCUS       DAOSIM010000303         2242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_41765_length_2242_cov_6.670782, whole genome shotgun sequence.
ACCESSION   DAOSIM010000303 DAOSIM010000000
VERSION     DAOSIM010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41765_length_2242_cov_6.670782"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    68..77
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <78..>2242
                     /gene="fdnG"
                     /locus_tag="PLR47_10200"
     CDS             <78..>2242
                     /gene="fdnG"
                     /locus_tag="PLR47_10200"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HOG13363.1"
                     /translation="SFGRGAMTNHWVDFKNSDVILIMGSNPASNHPVSWKWIQHAVDN
                     RGSKVICVDPRFTQSASKAHLYAPLRSGTDIAFLGGMIKYIIDNKLYFEEYVKNYTNA
                     AFLVNPAFKLPGDNKGVFSGFTNGKYEKDTWSYQTDGAGVIRKDPTMKNPNCVFQLLK
                     KHYSRYTPQLVSKICGTPVDKLIEVYKLYGSTGRPDRAGVDLYAMGWTQHTVGTQNIR
                     TMCMIQLLLGNMGVAGGGVAAMRGESNVQGSTDHGLLFHIWPGYLGTPSGSLATLKAY
                     NEKRTPKTKEANSLNWWKNFPKYSASFLRSMYGMNMGLEEAYAALPKVDDGANYSWLS
                     LFDQMYKGKFTGFFAWGMNPACSGAHSNKVRQALGKVDWMVNVNLFDNETGSFWRGPG
                     MDPAKIKTEVFMLPCAASIEKEGSIANSGRLQQWRYKAVNPVGDSLPDGDIMSEIFFK
                     VKELYEKKGGPHKEAITRLTWPYGKMAGKHFHYDPRKVAAEINGFFLADKKVENPTKK
                     GEFKEFKKGDLVPTFAWLQDDGSTSSGCWVYCGSVTADKGILPMRRDTADPTGLGLTP
                     GWAWSWPVNRRIIYNGASVDLNGKPWDPSRAVISWNGEKWTGDVPDGAGDPGKGRPPF
                     IMKPDGVASLYGPGLADGPFPEHYEPLECPVEKNLLSPQKNNPVIKRFDKPGVGSDLD
                     VYSNVGTCDPRFPFICSTYRVSEHWQTGVLTRWCPWLAE"
BASE COUNT          509 a          659 c          641 g          423 t
ORIGIN      
        1 taccaaaaat tcatgagggg gttgggcctg gtttacatcg aacatcaggc ccgtatctga
       61 cacagccnnn nnnnnnngtc gttcggacgc ggcgcaatga ccaatcactg ggtcgatttc
      121 aaaaacagtg acgtgatttt aattatggga agcaatcccg cttccaacca cccggtttcc
      181 tggaaatgga tacagcatgc cgtcgacaat cgcggttcaa aggtcatttg cgtggacccg
      241 cgctttaccc agtcggcctc caaggcgcat ctctacgcgc cccttcgttc gggaacggac
      301 atcgctttcc tgggcggcat gatcaaatac atcatcgaca acaaactgta cttcgaggag
      361 tatgtcaaaa attacaccaa cgccgccttc ctggtgaacc cggcgtttaa actgccgggc
      421 gacaacaagg gcgtgttctc cggctttacc aacgggaagt atgaaaaaga cacctggtcc
      481 taccagacgg acggcgcggg cgtgatccgc aaagatccga cgatgaaaaa ccccaactgc
      541 gtcttccagc ttttgaagaa gcactactcc cgctacacgc cccaactcgt cagcaagatc
      601 tgcggcacgc cggtcgacaa actcattgaa gtgtacaagc tttacggctc caccggcagg
      661 cccgaccgcg ccggtgtgga tctctacgcc atgggctgga cgcagcacac cgtcgggacg
      721 cagaacatcc gcaccatgtg catgattcag cttctgctgg gcaacatggg cgtcgcgggc
      781 ggcggcgtcg cggcgatgag aggcgaatcc aacgttcagg gctccaccga ccacggcctt
      841 ctgtttcaca tctggcccgg ctacctcgga acgccgagcg gatcgcttgc gacgctgaaa
      901 gcgtacaatg aaaagcggac gcccaaaacc aaggaggcca attccctcaa ctggtggaaa
      961 aacttcccga agtattcggc tagcttcctg cggtccatgt acggaatgaa catgggactt
     1021 gaagaggcgt atgccgctct gcccaaggtt gacgacgggg ccaactactc ctggctgtct
     1081 ctgtttgatc agatgtacaa gggcaagttc accggcttct tcgcctgggg aatgaatccg
     1141 gcctgttccg gcgctcactc caacaaggtc cgccaggcgc tgggaaaggt ggactggatg
     1201 gtcaacgtca acctgttcga caatgaaacc ggttctttct ggcgcggtcc cggcatggat
     1261 ccggccaaga tcaaaacgga agtcttcatg cttccctgcg ccgcgtccat cgaaaaggaa
     1321 ggcagcatcg ccaactccgg ccggctgcag caatggcgct acaaggccgt caatccggtg
     1381 ggcgattctt tgccggacgg cgacatcatg agcgaaattt tcttcaaagt aaaggagctc
     1441 tatgaaaaga agggcggccc gcacaaggaa gcgattaccc ggctcacctg gccctacggc
     1501 aagatggcgg gcaagcactt ccactacgat ccccgcaaag tagccgctga aatcaacgga
     1561 tttttcctcg cggacaagaa agtggaaaat ccgaccaaga agggcgaatt caaggaattc
     1621 aagaagggcg atctggtgcc cacgtttgcc tggctgcagg atgacggctc cacgtcatcg
     1681 ggctgctggg tgtattgcgg gtccgtgacg gccgacaagg ggattttgcc catgcgccgt
     1741 gatacggctg atccgaccgg tctggggctg acccccggat gggcctggtc gtggccggtc
     1801 aaccgccgca ttatttacaa cggcgcgtcg gttgacttga acggcaagcc ctgggatccc
     1861 agccgcgccg tgattagctg gaacggcgaa aaatggacgg gcgacgttcc ggacggagcg
     1921 ggcgatcccg gcaagggccg gccgcctttc atcatgaaac cggacggcgt ggccagcctg
     1981 tatggaccgg gactggccga cggtcccttc cccgagcatt acgaaccgct ggaatgcccg
     2041 gtggagaaaa acctgctgtc gccgcagaaa aacaatccgg tgatcaaacg gtttgataaa
     2101 ccgggcgtag gatccgacct cgatgtgtat tccaatgtcg gcacctgcga tccccgcttc
     2161 cccttcatct gctcgacgta ccgcgtcagc gaacactggc agacgggcgt gctcacccgc
     2221 tggtgtccgt ggctggcgga aa
//
LOCUS       DAOSIM010000304         2186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_43222_length_2186_cov_10.125763, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000304 DAOSIM010000000
VERSION     DAOSIM010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43222_length_2186_cov_10.125763"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="PLR47_10205"
     CDS             <1..739
                     /locus_tag="PLR47_10205"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOG13364.1"
                     /translation="LGIFAIAIGTAALPSFSAHAAAGKTEELKSSISFSLRLLLFLTI
                     PAMAALMALHLPIISVLFQRGAFDAQAAVYTGQALFCYALGLWAYSVLRVFVSAFYSL
                     KDSKWPLKAGIITLVVNVVFSLTLMYPLRHNGIALAGSISAAVNVGVLAFVLRNKIGP
                     YLDRAFYTSVFKIIASSAAMLAAIGLVEYGMPWNTSSGFRERLIYLVLAVLTGAVAYF
                     ACAYLLKSPEMRAFLKMIQKRLTGSGN"
     gene            833..2107
                     /gene="serS"
                     /locus_tag="PLR47_10210"
     CDS             833..2107
                     /gene="serS"
                     /locus_tag="PLR47_10210"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOG13365.1"
                     /translation="MLDIKYLRQNLEAVRRKMNERGQNIDFDRFIGLDAKRRDILQAV
                     ETLRNERNSVSRQVGELKKKKEDASALIAKMGDVSAKIKEYDETLRVTEEELNAFVMI
                     VPNVQHESVPQGGSSEDNAVVRTWGEKPSFCFAPKQHFELGESLNILDFGRGAKITGA
                     RFTLYRGAGAQLERAITNFMLDLHTANHGYTEVFTPFMVNAESMTGTGQLPKFKEDLF
                     KIEGMDYYLIPTAEVPVTNIYRDEILEEDKLPVYLVAYSACFRAEAGSYGKDTRGLIR
                     QHQFNKVEMVKFSKPEASYDELEKLTANAEEVLKRLGIPYRTVCLCTADLGFSSSKTY
                     DVEAWMPGQDTYREISSCSNFEDFQARRAAIRFRRKDSGKVEFVHTLNGSGLAVGRTV
                     VAVLENYQQADGSVIIPEALRPYMRGLERITP"
BASE COUNT          488 a          601 c          628 g          469 t
ORIGIN      
        1 gctggggatt ttcgccattg ccatcggaac ggcggcgctg cccagttttt ccgctcatgc
       61 cgccgcggga aaaaccgaag agctgaaatc cagcatttcc ttttccctgc ggctgctgct
      121 ttttctgacc atcccggcga tggccgccct gatggcgctc catttgccca tcatctccgt
      181 gctgttccag cgcggcgcgt ttgacgcaca ggcagccgtc tataccggcc aggcgctgtt
      241 ctgctacgcg ctggggctct gggcctactc ggtcctgcgc gtcttcgttt ccgccttcta
      301 ttccctgaag gattccaaat ggcctctgaa agcgggcatc attacgctgg tcgtcaacgt
      361 ggttttcagc ctgacgctga tgtatccgct ccggcacaac ggcatcgcgc tggccggctc
      421 catttccgcg gccgtcaatg tcggagtcct tgcgttcgtg ctgagaaaca aaatcggacc
      481 gtatctcgac cgtgcgtttt acacctccgt tttcaagatc atcgcttcat ccgccgcgat
      541 gctggcggcc atcggactcg tggaatatgg aatgccctgg aacacgtcct cgggctttcg
      601 ggaacgtctg atctatctcg tccttgctgt tctgaccggg gcagtcgcat actttgcgtg
      661 cgcctacctt ctcaaaagcc cggaaatgag agcctttttg aagatgattc aaaagaggct
      721 gaccggatcc gggaattgat tcacccgata aaaccattga cttcaattgt tcaaagtgat
      781 agggaaacgc gtctttggcg ggattcgtca gccaaagcac tgaaggtaaa ttatgttgga
      841 tattaagtac ttgaggcaga acctggaggc cgttcgccga aaaatgaacg aacgggggca
      901 aaacattgac tttgaccgtt ttatcggcct ggacgcgaaa aggcgggaca ttttgcaggc
      961 ggtggaaacc ctgcgcaatg agcgcaacag tgtgtccagg caggtcggcg agctgaaaaa
     1021 gaagaaagag gacgcttccg cactgattgc caaaatgggt gacgtctcgg ccaaaatcaa
     1081 ggaatatgac gaaaccctgc gcgtcacgga agaggagctc aatgcctttg tcatgatcgt
     1141 gccgaacgtt cagcacgaat cggtgccgca gggcggcagt tccgaagaca atgcggtcgt
     1201 gcggacctgg ggcgaaaagc cgtctttctg ttttgccccg aagcagcact ttgaactggg
     1261 tgaaagcctg aacattctgg atttcgggcg cggcgcgaaa atcaccggag cgagattcac
     1321 actctaccgg ggagcgggcg cccaactgga gcgcgcgatc accaacttca tgctggatct
     1381 gcacacggca aaccacggct acacggaggt gtttacgcct ttcatggtca acgccgaaag
     1441 catgaccggg accggccagc ttcccaagtt caaggaagac ctttttaaaa ttgaaggcat
     1501 ggactattac ctgattccga cggcggaagt cccggtgacg aatatctacc gggatgaaat
     1561 tcttgaggaa gacaagctgc ctgtttatct tgtggcttat tcagcctgct tccgcgcgga
     1621 agccggttcc tacggcaaag atacacgggg attgatccga cagcaccagt tcaacaaggt
     1681 ggagatggtc aagttttcca aaccggaggc atcctacgac gagctggaaa agctgaccgc
     1741 caacgcggaa gaagtcctga aaaggctcgg catcccttat cgcaccgttt gcctgtgcac
     1801 ggccgatctg ggcttctctt catccaaaac gtacgacgtg gaagcctgga tgcccggaca
     1861 ggacacgtac cgggagattt cctcatgcag caactttgag gatttccagg ccaggcgtgc
     1921 ggcgatccgc ttccgccgga aagacagcgg caaggtggaa ttcgtccaca ccctgaacgg
     1981 ctccggactg gccgtgggcc gcaccgtggt cgcggttctg gagaactatc agcaggccga
     2041 cggaagcgtc atcatcccgg aggcgctgcg tccttacatg agaggcctcg agcggatcac
     2101 tccgtaaggc cggaaggata accgggaaaa acattacgat ggagggatgg ccgagtggtc
     2161 tatggcggcg gtcttgaaaa ccgttg
//
LOCUS       DAOSIM010000305         2172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_43602_length_2172_cov_7.581483, whole genome shotgun sequence.
ACCESSION   DAOSIM010000305 DAOSIM010000000
VERSION     DAOSIM010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43602_length_2172_cov_7.581483"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2172
                     /gene="fdnG"
                     /locus_tag="PLR47_10215"
     CDS             <1..>2172
                     /gene="fdnG"
                     /locus_tag="PLR47_10215"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HOG13366.1"
                     /translation="GESFGRGSMTNHYCDLKNADVILIMGSNAAEHHPIAFKWIMRAK
                     EKGATVIHVDPRYTRTSARCDFHVPLRSGTDIAFLGGMIKYILENNKYFKDYVVNYTN
                     ASFLVNKDFSFNDGLFSGYDSQKRKYNKDTWTLDKDANGIPKRDMTLSDPRCVFQMLK
                     KHYSRYTLDKVSSITGVSKDNLLKVYKAYSATGAPDKAGTECYALGWTHHTVGVQNIR
                     SMAIIQLLLGNMGIAGGGINALRGEPNVQGSTDHCILYANLPGYLKMPAASLDTLEHY
                     LKKCTPESKDPQSANYYSNYPKFFVSFLKSLWDDKVTKENDFGYDWLPKIDDGKHYST
                     MHMFDAMYEGKIKGYFCVGADTAVSTPNSNKVRKAMENLDWLVGENIFNNETYEFWRG
                     PGVDPAKIKTECFLLPASASMEKEGSQSNSGRWVQWKYKAAEAPGDAIPVGEIEIKIM
                     AEVKKLYQKEGGPNASAITNLKWDYLDSKGHMDVLKVSHQVNGLFTKDTVIEDKAKGT
                     STLFKKGQCVPTFGNLKDDGSTACGNWIMAGSFDEKGFNKMTKRGKDDPTGLGLFPNW
                     SFAWPVNRRIIYNRASCDIYGKPYNPKRNILEWVGDKWVGDVPDGPWPPQADKAKGKY
                     PFIMKQDGVASLFGPAMAEGPFPEHYEPLESPLAKNPMSGQLNNPATEIFKGEMDKVA
                     SASEKFPYVCTTYSCTEHWCTGALTRWQAWLLEMQPELYVE"
BASE COUNT          595 a          537 c          545 g          495 t
ORIGIN      
        1 tgggagagtc gttcggacgc ggttccatga cgaaccacta ctgtgatttg aaaaacgcgg
       61 atgtcatttt gattatgggc agcaatgccg cggaacatca ccccatcgct ttcaagtgga
      121 ttatgagagc aaaagaaaag ggagcgacgg ttatccatgt cgatccccgt tatacaagaa
      181 catcggcgcg ctgcgatttc catgtgccgc ttcgctccgg taccgatatc gcttttctgg
      241 gcggtatgat caaatatatt ctggaaaaca ataaatactt caaagactac gtagtcaatt
      301 acaccaacgc gtcgttcctc gtaaacaaag atttttcttt taatgacggt cttttctccg
      361 gttacgattc gcaaaaacgc aagtacaata aagatacatg gactctcgat aaagatgcaa
      421 acggtattcc caaacgggac atgaccctgt ctgatccccg ctgtgttttc cagatgctga
      481 aaaaacatta ttcacggtat accctggata aggtttccag catcaccggc gtatccaagg
      541 acaacctcct caaggtgtac aaagcctaca gcgcgaccgg cgctccggac aaagccggta
      601 cggaatgcta cgctctgggg tggacacatc ataccgtggg cgtacagaac atccgctcca
      661 tggcgattat tcagttgctt ttgggcaata tgggtatcgc aggcggcggc atcaatgcgc
      721 tgcgcggcga acccaacgtt cagggctcga cagaccattg cattttatat gcaaatctgc
      781 ccggttatct gaagatgccc gcggcttctc tggacacgct ggaacactat ttgaaaaaat
      841 gcacgccgga atccaaggat ccgcagtcgg ccaattacta ttctaattac cccaaattct
      901 ttgtcagttt cttaaaatcc ctgtgggacg acaaggtgac aaaagaaaat gatttcggct
      961 acgactggct tcccaaaatt gatgacggga aacactattc cacgatgcac atgttcgatg
     1021 cgatgtatga aggcaagatc aagggatact tctgtgttgg cgcggatacg gccgtcagca
     1081 cgccgaacag caacaaggtt cgcaaggcga tggaaaatct ggactggctc gtgggtgaaa
     1141 atattttcaa caacgaaacc tacgaattct ggagaggccc cggtgtcgat cctgcaaaga
     1201 tcaagacgga atgcttcctg cttcccgctt ccgcgagtat ggaaaaggaa ggatcgcaga
     1261 gcaattccgg ccgctgggtt cagtggaaat acaaagctgc cgaagctccc ggcgatgcca
     1321 ttcccgtggg tgaaatcgaa attaagatta tggctgaagt caaaaagctt taccagaaag
     1381 aaggcggtcc caatgcctcg gctattacca atctcaagtg ggattatctg gacagcaagg
     1441 ggcacatgga tgtgttgaaa gtttcccatc aggtcaatgg cctctttaca aaagacacgg
     1501 ttatcgagga taaggcgaaa ggtacatcca ctttgtttaa aaaaggacag tgtgtcccga
     1561 cctttggaaa cctcaaggat gacggatcca cagcctgcgg taactggatt atggccggta
     1621 gttttgacga aaaaggtttc aacaaaatga ccaaacgcgg caaagacgat cccaccggtt
     1681 tggggctttt ccccaactgg tcattcgcct ggccggtcaa tcgccgcatc atttacaatc
     1741 gcgcttcctg cgatatttac ggcaaaccgt acaatcccaa acgtaatatt ctggaatggg
     1801 tgggagataa atgggttgga gacgtgcctg atggtccctg gccgccgcaa gcggacaagg
     1861 caaaaggcaa atatcctttt attatgaaac aagatggtgt ggcatctttg ttcggacccg
     1921 ccatggctga aggtcctttc ccggagcatt acgaaccgct ggaaagccct ctggctaaaa
     1981 atccgatgtc cggacagctc aacaacccgg ccactgaaat cttcaaaggt gaaatggata
     2041 aagtggccag cgccagcgag aagttcccct atgtctgcac gacgtattcc tgcaccgagc
     2101 actggtgcac cggcgcgctg acccgttggc aggcatggct tctggaaatg cagccggagc
     2161 tctacgttga aa
//
LOCUS       DAOSIM010000306         2162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_43872_length_2162_cov_6.669673, whole genome shotgun sequence.
ACCESSION   DAOSIM010000306 DAOSIM010000000
VERSION     DAOSIM010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43872_length_2162_cov_6.669673"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PLR47_10220"
     CDS             complement(<1..496)
                     /locus_tag="PLR47_10220"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOG13367.1"
                     /translation="MIPFRKPLLKTLKASTACFMVLVPSRENVVLEGPGSIRKLPDLM
                     KKQNIQKPLVITDRVLMEKHLPVSLFEAFKAAGMPYMVYDGVQPNPSIENVEEAYGLY
                     VKSNCDAVVGFGGGSSMDCAKVVAGRAVRPHLTAEQLGGYFKIMLPFPKKLPRIIAVP
                     TTAGT"
     gene            785..1393
                     /locus_tag="PLR47_10225"
     CDS             785..1393
                     /locus_tag="PLR47_10225"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG13368.1"
                     /translation="MKERNRNDLIHLRRVQLTRAAYKVVGEKGYSDFTIKDIAREAGL
                     STGLVHYYFKNKEDLLFKLLKGMNANLKSDLGKALSALEDPREKLLAFCNEAFDLVHK
                     EKAYFSVLVDFLAQINRNKRLRQAMAKLFQSYRDEIEAILQEGMAKGIFAVKDVRFTS
                     VTIVSLIQGNIFQHLIDQEAFDYSAYTEKTKKQIFALVMKDS"
     gene            1625..1903
                     /locus_tag="PLR47_10230"
     CDS             1625..1903
                     /locus_tag="PLR47_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13369.1"
                     /translation="MSREYKSGTYCVEVKCPKHKELEGLAGDAYLKKKKVHCKTCLAW
                     KFFNWAKDNKWRIVHTWPEVSNKQLAAMIKGIDPVRVEDLTEEEIMCL"
     gene            1947..>2162
                     /locus_tag="PLR47_10235"
     CDS             1947..>2162
                     /locus_tag="PLR47_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13370.1"
                     /translation="MRLAVRLAWPFIRAGKRLSNFPFLKWLIYPFFIRPYNEVSSIPL
                     NIEVQRPENIAMPRRVVKRLLANVDELF"
BASE COUNT          522 a          535 c          601 g          504 t
ORIGIN      
        1 ctgtgcctgc ggtggtcggc acggcaatga tgcgggggag tttcttcggg aacggcagca
       61 tgattttgaa gtatcccccc agttgctcgg ccgtcaggtg aggcctgacc gctcttccgg
      121 ctaccacctt tgcgcagtcc atggacgagc cgccgccgaa accgactacg gcgtcgcagt
      181 tgctttttac gtacagcccg taggcttcct cgacgttttc aatggaggga ttcggctgga
      241 cgccgtcata gaccatgtag ggcatgccgg cggctttgaa tgcctcaaaa agactgacgg
      301 gcagatgttt ctccatcagc acgcgatccg taatcacgag aggtttttgg atattctgtt
      361 ttttcatcag atcggggagc tttcgaatgc tccccggacc ttcaaggacc acgttttccc
      421 gtgaagggac aagaaccatg aagcaggccg tggaggcctt cagggttttg agaagcggct
      481 tccggaaagg aatcatattc tcttttctcc ttatgcgcaa aaggcatcgt gcttttgatg
      541 tggagcgagt ataggggaaa aagcggtggc ctgtcaacaa aaaggcgcga cggctcttca
      601 tgctccggca atatttcatt gacatcaaag tgaagttttc ctatgctcgc ttttccgtaa
      661 agacgggcgc ctctccagga ggcgctgccc cgcgatgcgc cggaaaattc cggccatgcg
      721 tcgggcggat ccaggatgcg gggattttga aaaagtagaa tttattgctt ctgacgggaa
      781 tatgatgaaa gaacgaaaca gaaatgattt gatccatttg agacgggttc agttgacccg
      841 ggcggcgtac aaagtggtcg gggaaaaagg ctattcggat ttcaccatca aggacatcgc
      901 gagggaagcc ggcctctcca cgggtctggt tcactattat ttcaagaaca aggaagatct
      961 cctgttcaaa ctgctcaagg gaatgaacgc caatctcaaa agcgatctgg gcaaggcgct
     1021 ttcggcgctg gaagatccgc gggagaagct cctggctttc tgcaacgaag cgttcgatct
     1081 ggtgcataag gaaaaagcgt atttcagcgt gctggtcgat tttctggcgc agattaaccg
     1141 caacaaacgg ctccgtcagg cgatggcgaa actttttcag agctaccgcg atgagattga
     1201 ggccattctt caggaaggca tggccaaggg aatctttgcg gtgaaggatg ttcgctttac
     1261 gtcggtcacc attgtttctc tgatccaggg gaatattttc cagcacctca ttgatcagga
     1321 ggctttcgat tattccgcct ataccgagaa aacaaaaaaa cagatttttg cgctggtcat
     1381 gaaggattca tgacgtcttc agggaaggct ttgcgggtcg cgcgcggcaa aggattcagt
     1441 ggagcagagc ccgccgtgcc ggccgcttcc gccgcttgga ggctttccgc caaactatcg
     1501 cttgacaact caaaacggac accctatagt cttgcaccaa aacagggaat ccgcggaatg
     1561 ccgaagacct gttcactgtc tcgtttccgg cgcagtccgg cagcggagaa aagagggacc
     1621 atccatgagc agagaataca aatcagggac atactgcgtc gaggtgaagt gccccaagca
     1681 caaggaactt gaggggcttg ccggggacgc atacctcaag aagaagaagg tgcactgcaa
     1741 gacgtgcctt gcctggaaat ttttcaactg ggcaaaagac aacaaatggc gcattgttca
     1801 tacttggccc gaagtctcca acaagcagct ggccgccatg atcaagggaa ttgatcccgt
     1861 tcgtgttgag gatttgaccg aagaagaaat catgtgcctc tagaagggac atttcaaact
     1921 tgcaatacgg tcccggtcga aattttgtga gactggccgt tcgactcgcc tggccgttta
     1981 ttcgggcggg gaagcggctc agcaatttcc cctttttgaa atggctgatt tatccttttt
     2041 tcatccggcc gtacaatgaa gtgagctcca ttccccttaa tattgaagtt cagcgccccg
     2101 aaaacattgc gatgccccgc cgcgtcgtca aaagacttct ggcgaatgtg gacgagctgt
     2161 tc
//
LOCUS       DAOSIM010000307         2146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_44332_length_2146_cov_7.928264, whole genome shotgun sequence.
ACCESSION   DAOSIM010000307 DAOSIM010000000
VERSION     DAOSIM010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44332_length_2146_cov_7.928264"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PLR47_10240"
     CDS             complement(<1..698)
                     /locus_tag="PLR47_10240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG13371.1"
                     /translation="MKTTSPKTKTARKRAEEALRHNEEKYREMIETIQDGYFEIDLTG
                     KYTFVNDVICRQLQYSREELIGSDNRKYQDEENAKKTFQAFLDAYTTGKPVPALEMDV
                     IRKDGSTQIAEVSISLIRDAGGQPLGFRGISRDITERKQATQALLESEALQRHLMESL
                     PIGLVVVDPRTRMIEHANQTASVMFGAPVDQIVGHRCHAFLCPAQEGACPVCDLGKDV
                     DNSERWMVCADGHHR"
     gene            complement(818..1798)
                     /locus_tag="PLR47_10245"
     CDS             complement(818..1798)
                     /locus_tag="PLR47_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HOG13372.1"
                     /translation="MKYIDHGKGGPPSCMILREGPVPRLKAGEALIEVAFAGVNRPDV
                     FQRMGAYPPPPDASPILGLEVAGRIAAVADDVTEWTIGDPVCALSPGGGYAEYCAVPA
                     LHCLPVPSGLSQAQASALPENYFTVWSNVFDRGALTPGENFLVHGGAGGIGYTAIQLA
                     KAFGAVVFTTAAGADQMEAVKKLGADVALDYTTQDWQREIENRIGKRSIDCILDFVGG
                     PYIQKNLRLLARNGRLVQIAFMQGAKVELDCLPILTQNLTFTGSMLRPRSSEEKASIA
                     RALHKNVWPLFAAGKLRVLISAVFELAEAAQAHTLMESAAHVGKIILKVR"
BASE COUNT          433 a          611 c          608 g          494 t
ORIGIN      
        1 cggtgatggc cgtccgcgca gaccatccat cgttcggaat tgtcgacgtc tttgcccagg
       61 tcgcagacgg gacaggcgcc ttcctgggcc gggcacagaa aagcatggca ccggtgtccg
      121 acgatctggt ccaccggggc gccgaacatc acggaagcgg tttggttggc gtgctcaatc
      181 atgcgcgtcc gcgggtccac gaccaccagc ccgatgggca gagattccat caggtgacgc
      241 tgcagcgctt cgctctccag aagggcctgc gtcgcctgtt tgcgctccgt gatatcccgg
      301 gagatgccgc gaaaccccag gggttgtccc ccggcgtccc gaatgaggga tatcgacact
      361 tcggcaattt gcgtcgagcc gtctttgcgg atcacgtcca tttccagcgc cggcaccggt
      421 ttgcccgtcg tgtaagcgtc caaaaaagcc tggaaagttt ttttggcgtt ttcctcatct
      481 tgatacttgc ggttgtccga tccgatcaat tcttcgcggg agtattgcag ctgccggcag
      541 atcacatcat tgacaaaagt gtatttgccg gtcagatcaa tctcgaaata accgtcctgg
      601 atggtttcga tcatctcgcg gtatttttct tcattgtgcc gcagggcctc ttccgcgcgc
      661 ttgcgcgcgg tttttgtttt cggggaggtt gttttcattg gtcatcgcca tccgttgcgt
      721 gataaagggt tcatgacgta tcatttcaac ctgaacacac aactacctga aaaaacgttt
      781 caagtaaagg ccgcaaaatc attgccggca gacgcgttca gcgcactttc aggatgatct
      841 tgccgacatg agcggccgat tccatgaggg tatgagcctg cgcggcttcg gcaagctcaa
      901 agacggcgga gatcaaaacc cgcagctttc ccgcggcaaa gagcggccag acgtttttgt
      961 gaagggcccg ggcgatggag gccttttcct ccgaagagcg gggacgaagc atcgagccgg
     1021 tgaaggtcag attttgggtc aaaatcggca ggcagtccag ttccactttc gcgccctgca
     1081 tgaaggcgat ctgcaccagc cttccgtttc gggccagcag cctcagattc ttttgaatat
     1141 aggggccgcc cacgaaatcc agaatacagt cgatgctcct cttcccgatc cggttttcaa
     1201 tttcccgctg ccagtcctgc gtcgtataat ccagcgcaac atccgccccc agttttttca
     1261 cggcttccat ctgatcggcg ccggccgcgg tggtaaagac cacggcgccg aaggccttgg
     1321 ccagttgaat ggcggtatag ccgatgcctc ccgcgccgcc gtgaaccaga aaattttcgc
     1381 cgggcgtgag agcgccgcgg tcaaagacgt tggaccacac ggtgaagtag ttttccggaa
     1441 gcgcgctcgc ttgagcctgc gaaagccccg aaggaaccgg caggcaatga agcgcgggca
     1501 cggcgcaata ctcggcatat ccgccgccgg gcgaaagcgc gcaaaccgga tctccgatgg
     1561 tccattccgt tacgtcgtcc gcaacggctg cgattcggcc cgcgacttcg aggcccagga
     1621 tgggggacgc gtcgggcggc gggggataag cccccatgcg ctggaagaca tccggacggt
     1681 tgacgcccgc gaaggcgact tcaatcagcg cttctccggc tttgagccgc ggcacgggcc
     1741 cttcccgcag gatcatgcag gaaggcgggc cgccttttcc gtggtctata tatttcatga
     1801 cggatccttt taaaactttt ttgcgtccgg ccgggggcgg cattccccgt gcgggacgac
     1861 gctccgcgaa ccggcctgtc tgttcaaaat gccgtccgat tttagcacag ccgcaagaaa
     1921 aagacacggc gtcttgtgct gattcattcc ggtatcgctt taaattttct tttgtttcct
     1981 cgaaagaatt tttttgtcgt gaaattttct tgacaggaaa tttttgcttc tatatgctcg
     2041 gtttccaatt tacgtcaata agaagcagat gtatctaacc ctctagttag caacagtagt
     2101 ggcgtggtga tcctatggac atgaagcgcg attactatga agacat
//
LOCUS       DAOSIM010000308         2116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_45207_length_2116_cov_6.971858, whole genome shotgun sequence.
ACCESSION   DAOSIM010000308 DAOSIM010000000
VERSION     DAOSIM010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45207_length_2116_cov_6.971858"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PLR47_10250"
     CDS             complement(<1..466)
                     /locus_tag="PLR47_10250"
                     /inference="COORDINATES: protein motif:HMM:NF018827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13373.1"
                     /translation="MTETKIPLDLILERMAEDAEKARQRASRASDVLLGELHYEIGST
                     PYEIVYEEDRVRLKHYLRNEEAENKPKTPLLVVYALINRETMLDLQPDRSVVKTFLQE
                     GIDLYMVDWGYPTSKDRFLTIDDHVNGYMDNIVDFLRRKHGVPKINLMGICMG"
     gene            complement(525..1436)
                     /locus_tag="PLR47_10255"
     CDS             complement(525..1436)
                     /locus_tag="PLR47_10255"
                     /inference="COORDINATES: protein motif:HMM:NF021246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HOG13374.1"
                     /translation="MADAGNPFPFHESSVFPWLKTAADMWLNLAKTLPPNSDTTLKTQ
                     TAVQNRFTQQLETSLNLFKSFSRMMNEPESASATANTMNALPEIFLKMAGSGFEAAMQ
                     IQNHLMEKAARIGKRTEAYNFDNLDQEVFRALSEVYEKELRQYLKIPPLGLTRFYQER
                     FNEMLDKHNLFETTLAEFLSILYLPMEKSFNVLQEKLKQRTEEGNIPHDVKESYAMWL
                     KILEGHYMNLFKSKEYTDALHRTLNKLEDFLIAKNEAMRDFLQLLPVVTQKDMDEMYK
                     EFHLLKKRVKALEKKAGISPNTLTVVK"
     gene            complement(1531..>2116)
                     /locus_tag="PLR47_10260"
     CDS             complement(1531..>2116)
                     /locus_tag="PLR47_10260"
                     /inference="COORDINATES: protein motif:HMM:NF015892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HOG13375.1"
                     /translation="NKVAHDLTQWHNPGNVKMKFASPPERMSMKNTLVFIHGLESTSQ
                     GNKAQYFRKVFPEMIIEDYTGSLAARMQKLKKVLEGKTNLILVGSSYGGLMAARFALD
                     SEPMIRKLILLAPALTIEGFEQAVRQKLQIPTVLYHGTRDDVVPPQAVKAIAEKTFAR
                     LEHHFVEDDHPLNNVFPTLPWPLLLEQGPAAETD"
BASE COUNT          409 a          529 c          596 g          582 t
ORIGIN      
        1 cgcccatgca gatgcccatc agattgattt tggggacgcc gtgtttccgc cggaggaagt
       61 ccacgatgtt gtccatgtag ccgttgacgt gatcgtcgat ggtcagaaat ctgtctttgc
      121 tcgtcggata gccccagtcc accatgtaca ggtcgatgcc ttcctgtaga aaggttttta
      181 cgacgctgcg gtccggctgc aaatccagca tcgtttcgcg gttgatcagc gcgtacacca
      241 ccagaagggg cgttttcggc ttgttttccg cctcttcatt gcggaggtag tgcttgaggc
      301 ggacgcggtc ttcttcgtaa acgatttcat aaggcgtcga tccgatttcg tagtggagct
      361 ctcccagcag gacgtcggaa gcccgcgaag cccgctgccg ggccttttcc gcgtcctcgg
      421 ccattcgttc cagaatcaga tccaggggaa tttttgtttc ggtcatgaaa acgccatcct
      481 tccgaaaact tttttcccgc ccggggcagg gggcggaaaa aagtttactt gacgaccgtc
      541 agggtgttgg gggatatgcc ggcttttttc tccagcgctt tgactctctt tttgagcagg
      601 tgaaactcct tgtacatctc atccatatct ttttgcgtga ccacgggcag caattggaga
      661 aagtcacgca tggcttcatt cttggcgatg aggaaatcct ccaatttgtt gagcgtgcgg
      721 tgcagcgcgt ccgtgtattc tttggacttg aacagattca tgtagtggcc ttccaggatt
      781 ttcagccaca tggcgtagct ttccttcaca tcgtggggga tgtttccctc ctccgtccgc
      841 tgtttgagct tttcctgaag cacattgaag gatttttcca tcggcagata caggatggat
      901 agaaattccg ccagcgtggt ttcaaagagg ttatgcttgt ccagcatctc gttgaagcgc
      961 tcctggtaaa aacgcgtcag tcccagcggc ggaatcttca ggtattggcg aagttctttt
     1021 tcatagactt cactgagcgc ccggaagact tcctgatcca gattgtcgaa gttgtaggct
     1081 tccgtgcgct tgccgatccg ggcggctttt tccatgagat ggttctggat ctgcatggcc
     1141 gcttcaaagc cggatccggc catcttcaga aaaatctccg gcagcgcgtt catcgtattg
     1201 gcggtggccg aagccgattc cggctcgttc atcattcggg agaaggattt gaagagattc
     1261 aggctggttt cgagctgctg cgtgaaacgg ttttgcacgg cggtttgcgt tttcagcgtt
     1321 gtgtccgaat tgggcggaag ggttttggcc aggttgagcc acatatccgc ggcggttttc
     1381 aaccacggaa aaacggacga ttcgtgaaag gggaaggggt tgcctgcgtc agccatgata
     1441 acctcttttc ggagccgcgc ttctgaaggg attgttgggt tcccttcggg caggcccgcg
     1501 gtttttttgt aatctatccg ccggtgtttg tcaatcggtt tcggccgccg gcccctgttc
     1561 caggagcaaa ggccagggga gcgtcggaaa aacgttgttg agcggatggt cgtcctccac
     1621 gaagtgatgc tccaggcgcg cgaatgtttt ttcggcgatg gctttaacgg cctggggagg
     1681 gacaacatcg tcccgggttc cgtgatacag gaccgttgga atttgcagtt tttgccgaac
     1741 ggcctgttca aatccttcaa tggtaagggc gggggcaagc agaatcagct ttcgaatcat
     1801 tggttcgctg tccagcgcaa agcgggccgc catcaggcct ccgtagctgg atcccaccag
     1861 aatcaaattg gttttgccct caagaacctt tttgagcttt tgcattcggg ccgcgaggct
     1921 gcccgtgtaa tcctcaatga tcatttccgg aaaaactttt cggaagtact gagctttgtt
     1981 tccctgggaa gtgctttcca gaccgtgaat aaaaaccagc gtgtttttca tggacatcct
     2041 ttccggcggt gaggcgaatt tcattttgac gttgcccgga ttatgccatt gtgttaaatc
     2101 gtgcgcaact ttattt
//
LOCUS       DAOSIM010000309         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_45355_length_2111_cov_7.342899, whole genome shotgun sequence.
ACCESSION   DAOSIM010000309 DAOSIM010000000
VERSION     DAOSIM010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45355_length_2111_cov_7.342899"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..410
                     /locus_tag="PLR47_10265"
     CDS             114..410
                     /locus_tag="PLR47_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13376.1"
                     /translation="MKETLRIVGKNSVESSVGWKIELLSPEILAYREGEKSIHLEMED
                     RPDVGGELEWIIYMPEYWTWREKDEPLAREKVPEILKRIDLAFWKLDLKIKEIV"
     gene            complement(452..2098)
                     /gene="ilvD"
                     /locus_tag="PLR47_10270"
     CDS             complement(452..2098)
                     /gene="ilvD"
                     /locus_tag="PLR47_10270"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOG13377.1"
                     /translation="MKKGLERAPHRSLFKAMGYTDEEIARPLIGVVNSANEIIPGHIH
                     LDLITQDVKAGIRMAGGTPVEFPVIGVCDGIAMNHAGMKYSLASRELIADSIEIMAMA
                     HPFDALVFIPNCDKIVPGMLMAALRLNIPSIFISGGPMLAGKIQGKSVDLISVFEGVG
                     AVKAKKMNSSQLQQLEDCACPGCGSCSGMYTANSMNCVTEALGLGLPGHGTIPAVLSA
                     RHRLAKYAGMKIMDLLKKNVKPRDIATPAAFKNAIAVDMALGCSTNTVLHIPAVAHEA
                     GITLDLNLFNKISAKTANICKLSPAGPHHIEDLDAAGGIQAVMKTIAGLGVIDEKCLT
                     VSGKKVGANLKTARVLNEAVIRPLNNPYSPQGGIAILRGNLAPDGAVVKQSAVAPAMQ
                     VNEGRARVFDSEDRAIAAIMDRKIKPGDIVVIRYEGPKGGPGMREMLSPTSAIVGMGL
                     DKSVALLTDGRFSGGTQGAAIGHISPEAAEGGPIALVKEGDIIAIDIPAKTLTLKVDE
                     AELDKRRRTLKPWTQAITTGYLARYAKMVTSASTGAVFKD"
BASE COUNT          426 a          584 c          651 g          450 t
ORIGIN      
        1 ctacggcgaa gggctgatgg actacaacaa aagcacgaac cggatcagct tcggtgtgat
       61 gatcgctgaa tggaactgac gcgggtgaat gctccgccat cggaatggga atcatgaaag
      121 aaactttgcg aattgtcggc aaaaacagcg tggaaagctc tgtgggctgg aaaattgagc
      181 tcctttctcc ggaaatcctc gcgtaccggg aaggagaaaa aagcatccac ctggagatgg
      241 aagaccggcc cgacgtcgga ggagaactgg agtggatcat ttacatgccg gaatactgga
      301 cctggcgtga aaaggacgaa ccgctggcgc gggaaaaggt gcccgaaatt ttaaaaagga
      361 tcgatctggc cttctggaag ctggatttga aaatcaaaga aatcgtctga aaaaaaaggc
      421 ccggctcacg ccgggccctg ccggttgtga attaatcttt gaacaccgcc cccgtagaag
      481 cggaagtgac catttttgca tagcgcgcca gatagccggt ggtgattgcc tgcgtccagg
      541 gtttgagcgt cctgcggcgc ttgtccagtt ccgcctcatc caccttcaac gtcagcgtct
      601 tggccggaat gtcgatggcg atgatatcgc cttccttcac cagcgcaatc ggcccgcctt
      661 ccgccgcttc cggggaaatg tggccgatgg ccgcgccctg cgtgccgccg ctgaagcgcc
      721 cgtcggtgag cagtgcaacg gatttgtcca ggcccatgcc gacgatcgcc gacgtggggg
      781 agagcatttc gcgcatgccc ggcccgcctt tgggaccttc gtagcgaatg acgacgatgt
      841 cgcccggttt gatctttctg tccatgatgg cggcaatggc cctgtcctcg gaatcgaaca
      901 cgcgggccct gccttcgttc acctgcatgg cgggcgccac ggcggactgc ttgaccaccg
      961 cgccgtccgg cgcgaggttc ccccgcagaa tggcgatgcc gccctgcgga gaatagggat
     1021 tattgagcgg ccggatgacg gcttcgttga ggacccgggc ggtctttaaa ttggcgccca
     1081 ctttttttcc gctgacggtc aggcacttct cgtcgatgac gcccagtccg gcgatcgttt
     1141 tcatgacggc ctggatgccg cccgccgcgt cgaggtcttc gatgtggtgc ggcccggcgg
     1201 gactgagttt gcagatattg gctgtttttg cgctgatttt gttgaacaga ttcagatcca
     1261 gcgtgatgcc cgcttcgtgg gcgacggccg gaatgtgcaa aacggtgttg gtggagcagc
     1321 ccagcgccat atcgacggca atggcgtttt tgaaggcggc tggcgtcgca atgtcgcggg
     1381 gtttgacgtt ctttttgagc agatccatga ttttcatgcc cgcgtatttg gccaggcgat
     1441 gccgggcgga aagcaccgcc ggaatcgtgc cgtgcccggg tagcccaagg cccagggctt
     1501 ccgtgacgca gttcatcgag ttggccgtgt acatgccgga acaggacccg cagccggggc
     1561 aggcgcagtc ttccagttgc tgcagttgcg acgaattcat ctttttggcc ttgaccgcgc
     1621 cgacgccttc aaacacggag atgagatcga cggatttgcc ctgaatcttg cccgcgagca
     1681 tcgggccgcc gctgataaaa atggacggaa tgttgagcct cagagccgcc atgagcatgc
     1741 cgggaacaat cttgtcgcag ttgggaatga acaccagcgc gtcgaacgga tgggccatcg
     1801 ccatgatctc aatggaatcg gcgatcagct cccggctcgc cagagaatat ttcatgccgg
     1861 cgtggttcat ggcgatgccg tcgcagacgc cgatgacggg aaattcaacg ggggttcccc
     1921 cggccatgcg gatgcctgct tttacgtcct gcgtaattaa atccagatga atatggccgg
     1981 gaatgatttc attggcggaa ttgaccacgc cgatgagcgg ccgggcaatt tcttcatcgg
     2041 tatatcccat tgctttaaag agcgaacggt gcggcgcccg ctccagtccc tttttcatga
     2101 gatcgcttcg c
//
LOCUS       DAOSIM010000310         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_45356_length_2111_cov_7.257296, whole genome shotgun sequence.
ACCESSION   DAOSIM010000310 DAOSIM010000000
VERSION     DAOSIM010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45356_length_2111_cov_7.257296"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1588
                     /locus_tag="PLR47_10275"
     CDS             <1..1588
                     /locus_tag="PLR47_10275"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOG13378.1"
                     /translation="RVIAGHWGLIPKMQKLALAGKIEAYNFPQGVITHMYRDVAGRLP
                     RTITHVGLGTFVDPRLSAGKVNAVTTEDLVERITFDGREYLAYKTLPVSVAFLRGTTA
                     DMDGNISMEKEPLTLEVLPMAMAAKNSGGFVIVQVERIADRHALNPRHVRIPGILVDC
                     VIVARPENHWQTYVTHYNPAFSGEFKIPTATVEPLPMDERKLVARRAAFELRPNMVVN
                     LGGGGDFGTATNMDFLNDMHAQFDFYDGGGLDLACLGLAQMDSHGNVNVSKFGPKLAG
                     CGGFINITQNAKKIVFAGTFTAGGTRLAVENGKLKIVQEGSLKKIVREVEQITFSGET
                     ARQGEQQVFYVTERCVFRLTREGVELTEIAPGVDLEKDILAQMEFRPVMKNVRPMDER
                     IFRLPLMGLKDDLLSIPIPERLTYDPATNIFYVNFEGLHVRSSADIEAIRSRVAEVCA
                     PLGKRVKTIVNYDNFSIAPDLEDEYVKMVKYVVSEYYSDVTRYTTSAFLRMKLGDELK
                     KRNLAAHIFQSKEEAREALE"
     gene            1752..>2111
                     /locus_tag="PLR47_10280"
     CDS             1752..>2111
                     /locus_tag="PLR47_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOG13379.1"
                     /translation="MPIEVKLEGHVAVVTLNEGENRLNLDFLQKFIDTLDHVEKQTQA
                     TVLVVRGADAKIFSNGIDLEWLMPIMQKNDTATAKKFIETMMALFRKIALYPLTTIAA
                     MTGHAFAGGAIMCCYFDF"
BASE COUNT          520 a          567 c          625 g          399 t
ORIGIN      
        1 gcgcgtcatt gccgggcact ggggtttgat ccccaaaatg cagaagctcg ccctcgcggg
       61 aaaaatcgaa gcctacaact ttccccaggg agtgattacg cacatgtacc gggatgtcgc
      121 gggacgcctg ccccggacga tcacgcacgt gggattgggc accttcgtcg atccccggct
      181 gtccgccggc aaggtcaacg ccgtcaccac ggaagacctg gttgaacgga tcacctttga
      241 cggcagggaa tatctggcct acaagaccct gccggtttcg gtggcgtttc tgcgcggcac
      301 cacggcggac atggacggca acatttcgat ggaaaaggag ccgctgacgc tggaagtgct
      361 gccgatggcc atggcggcta aaaactctgg cggattcgtc atcgtgcagg tggaaaggat
      421 cgccgaccgc cacgcgctca atccccgcca tgtcaggatt cccggaattc tggtggactg
      481 cgtaattgtg gccaggccgg aaaatcactg gcaaacctat gtcacgcact ataatccggc
      541 cttcagcggc gaattcaaga ttcccacggc caccgtggag ccgctgccca tggacgaacg
      601 gaagcttgtc gcgcgccgcg ccgcctttga gcttcggccg aacatggtgg tgaatctggg
      661 cggcggcggg gatttcggca ccgccaccaa catggatttt ctcaacgaca tgcacgccca
      721 gtttgatttt tatgacggcg gcgggctgga cctggcctgc ctggggctgg cccagatgga
      781 ttcccacggc aacgtgaacg tcagcaagtt cggtcccaaa ctggccggct gcggcggctt
      841 catcaacatc acgcaaaacg ccaagaaaat cgttttcgcg ggaacgttta ccgccggcgg
      901 aacccggctg gccgtggaaa acggcaaact gaaaatcgtg caggagggga gcctgaaaaa
      961 aatcgtgcgg gaagtggagc agatcacctt cagcggcgaa accgcccggc agggtgaaca
     1021 gcaggtgttt tacgtcaccg aaagatgcgt attccggctg acgcgcgaag gcgtggagct
     1081 taccgaaatc gctcccggcg tcgacctgga aaaagacata ctggcgcaga tggaattcag
     1141 gccggtcatg aagaatgtcc gcccgatgga cgagcggatc ttcaggctcc cgctgatggg
     1201 gctcaaggat gatctcctga gcattccgat tcccgaacgt ctcacctacg atccggcgac
     1261 caacatcttt tatgtcaatt tcgagggcct ccatgtccgc agctcggccg acatcgaggc
     1321 gatccgctcc cgcgtggcgg aagtgtgcgc tccgctgggg aagcgcgtca aaacgatcgt
     1381 caactacgac aacttctcga tcgcgccgga cctggaagat gaatacgtca aaatggtcaa
     1441 atatgtggtg tccgaatact acagcgacgt cacccgctac accaccagcg cgtttctgcg
     1501 catgaagctg ggcgatgagc tgaaaaaacg gaatctggcg gcgcacattt tccagtccaa
     1561 ggaagaggcg cgggaggcgc tggaataaga aaggctgcaa agactgaagg ctgttgggct
     1621 gaaggcaaag caagaatgga aattggggtt gaaaatattg ccgcaaaaag ataagggtca
     1681 cagagagtaa aaaaaggtcg tacaaacggt ccggaaccgt taaaaaaaat attgaagaaa
     1741 gaagaagaat tatgccgatt gaagtcaagt tggaaggcca tgtggccgtt gtgacgctga
     1801 acgaagggga aaaccgtttg aacctggatt tcctgcagaa atttattgac acgctggatc
     1861 acgtggaaaa acagacccag gccaccgtgc tggttgttcg gggagcggat gccaaaattt
     1921 tcagcaacgg cattgatctg gaatggctga tgccgatcat gcagaaaaac gacacggcca
     1981 cggccaagaa atttatcgaa acgatgatgg cgctgtttcg gaaaattgcc ctctatccgc
     2041 tgaccaccat cgccgcaatg accgggcacg cctttgccgg cggcgccatc atgtgctgct
     2101 attttgattt t
//
LOCUS       DAOSIM010000311         2107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_45467_length_2107_cov_7.509747, whole genome shotgun sequence.
ACCESSION   DAOSIM010000311 DAOSIM010000000
VERSION     DAOSIM010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45467_length_2107_cov_7.509747"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /gene="rpsJ"
                     /locus_tag="PLR47_10285"
     CDS             <1..217
                     /gene="rpsJ"
                     /locus_tag="PLR47_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOG13380.1"
                     /translation="TGARVAGPIPLPTEINKYCVNRSPHVDKKSREQFEIRTHKRLID
                     IVEPTQATVDALMKLDLSAGVDVEIKA"
     gene            313..945
                     /gene="rplC"
                     /locus_tag="PLR47_10290"
     CDS             313..945
                     /gene="rplC"
                     /locus_tag="PLR47_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOG13381.1"
                     /translation="MNKGILGKKLGMTQVFAEDGAAIGVTAIEVEPSVVVQVKTMEKD
                     GYEAVQIGYGRMKQKNVTKPLQGHLNKAGKGFFRVLREIPAAAGDYEVGQEISADIFQ
                     AGGFVDVVGTSKGKGFQGVMKRHGFGGGRATHGSMFHRAPGSIGASADPSRVFKGTKM
                     GGHMGDVRKTVQNLQIWQIRSDRNLLLLKGSVPGGKNGYVFIRQARKKSA"
     gene            964..1587
                     /gene="rplD"
                     /locus_tag="PLR47_10295"
     CDS             964..1587
                     /gene="rplD"
                     /locus_tag="PLR47_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOG13382.1"
                     /translation="MALADVFDIEKKKVAEVELNDAVFGADVNEATIYEVVKMQRASR
                     RSGTSATKGRNDVNGGGKKPWRQKGTGRARSGTSRSPIWRGGGIVFGPQPRDYSYGVP
                     KKVRKNALISALSLKVKEDKITILRDFPMEAISTKAFRKVVDLFGLTKALFVIDRDNA
                     VLLKSSRNLKNVKMIRSEGINVYDVLNYEHLVLLEPSIKKIEGALLA"
     gene            1587..1874
                     /gene="rplW"
                     /locus_tag="PLR47_10300"
     CDS             1587..1874
                     /gene="rplW"
                     /locus_tag="PLR47_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOG13383.1"
                     /translation="MELHQVIKKILLTEKSNLDREAANKYHFEVDRRANKVEIGQAVE
                     KLFKVKVTQVRVIQVLGKKKRMGRVTGQKSSWKKAIVTLAQGSRIEVAEGV"
     gene            1896..>2107
                     /gene="rplB"
                     /locus_tag="PLR47_10305"
     CDS             1896..>2107
                     /gene="rplB"
                     /locus_tag="PLR47_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416217.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOG13384.1"
                     /translation="MAIIKYKPTSPGRRFQSVSDFAEITSAEPVKSLLKPIKRTGGRN
                     NNGRITARFIGGGHKRKYRMIDFCRN"
BASE COUNT          558 a          516 c          638 g          395 t
ORIGIN      
        1 gaccggcgct cgcgtcgccg gtcccattcc tcttcctacg gaaatcaata aatactgtgt
       61 caatcgttcg ccgcacgtgg ataagaaatc acgggagcag ttcgaaattc gcacacacaa
      121 aagattgatt gatattgtcg agccgacgca agccacggtg gatgcgctga tgaaactgga
      181 tctatcggcg ggagtggatg tcgagataaa agcgtaggct gaaaaagaga ggcggtggaa
      241 agtggccgtt ggggatgcca tttttccaaa acgtgagctt cgcggcttta ttcattgaaa
      301 agttggatga tgatgaacaa gggaattctg ggtaagaaat taggcatgac gcaggtattt
      361 gccgaagacg gcgccgccat cggtgtgacg gccattgaag tcgaaccgtc ggtcgtcgtt
      421 caggtgaaaa ccatggaaaa ggacggctac gaagcggttc agatcggcta cggccggatg
      481 aaacagaaga atgtcaccaa gccgctgcag ggccatttga acaaggccgg caaaggattc
      541 ttccgcgttc tgagggaaat tcccgccgcg gccggcgact acgaagtcgg ccaggaaatt
      601 tccgcggaca tttttcaggc gggcggcttc gtggatgttg tgggcacctc gaaaggcaaa
      661 gggtttcagg gcgtcatgaa gaggcacggc ttcggcggcg gccgggcgac gcacggctcc
      721 atgtttcacc gggcccccgg ttccatcggc gccagcgcgg atccgtcccg tgtgttcaaa
      781 ggcaccaaaa tgggcggcca catgggcgat gtccgcaaaa ccgttcaaaa cctgcaaatc
      841 tggcaaatcc gctccgacag aaacctgctg ctcctgaaag gttccgttcc cggcgggaaa
      901 aacggctatg tgttcattcg gcaggccagg aaaaaaagcg cgtaggacag tggagtaatc
      961 agcatggctc tcgcagatgt ttttgatatt gaaaaaaaga aggttgccga agtcgaattg
     1021 aacgacgcgg ttttcggcgc ggacgtgaac gaagcaacga tctatgaggt cgtaaagatg
     1081 cagcgggcat cgcggcgctc gggcacctct gccaccaagg gaagaaacga cgtgaacggc
     1141 ggcggaaaga agccgtggcg ccagaagggg acgggtcgcg cccgctcggg aacgtcgcgg
     1201 tcgcccatct ggaggggcgg cgggatcgtg tttggacccc agccccgcga ttattcgtac
     1261 ggcgttccca agaaggtccg gaaaaacgcg ctgatttccg cgctgagcct gaaggtgaaa
     1321 gaagacaaaa taacgatcct gcgcgatttt ccgatggaag cgatcagcac caaggcgttc
     1381 cggaaagtgg tggatctttt cggtctcacc aaagcgcttt ttgtgattga ccgggataac
     1441 gccgtgctgt tgaaatcgtc gcgcaacctc aaaaacgtga aaatgattcg gtcggaaggc
     1501 atcaatgttt atgatgtgct caactacgag cacctggtcc ttcttgaacc ctccataaaa
     1561 aagatcgagg gggcattgct ggcataatgg aactgcatca ggtgatcaaa aaaatcctcc
     1621 ttacggaaaa gagcaacctg gaccgtgaag cggcaaacaa ataccatttt gaagtagatc
     1681 gccgcgccaa caaggtggaa atcggccagg cggtggaaaa attattcaag gtcaaagtga
     1741 cccaggttcg cgtgattcaa gttctgggca agaagaagcg catgggccga gtgacggggc
     1801 agaagagctc ctggaaaaag gcaatagtga cgctggccca aggcagccgc atcgaggtcg
     1861 ccgaaggcgt atagaacgag tgaaggaata agcacatggc aatcatcaag tataaaccga
     1921 cgtcaccggg caggcggttc cagagcgttt cggatttcgc ggagatcacc tccgccgagc
     1981 cggtcaaaag ccttttaaaa ccgattaaaa gaacgggcgg ccgcaacaac aacgggcgca
     2041 ttacagcgcg ctttatcggc ggcggccaca agcgcaagta ccggatgatc gatttttgcc
     2101 gcaacaa
//
LOCUS       DAOSIM010000312         2092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_45937_length_2092_cov_8.540010, whole genome shotgun sequence.
ACCESSION   DAOSIM010000312 DAOSIM010000000
VERSION     DAOSIM010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45937_length_2092_cov_8.540010"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..1472
                     /locus_tag="PLR47_10310"
     CDS             231..1472
                     /locus_tag="PLR47_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HOG13385.1"
                     /translation="MDFKPRSHEKIQQLLDKGVNIPNPATLDIGDDVNIDQISGKGTA
                     IYPGCRIYGGKTVISSGCKLGYEAPATIEDCQIGERVELKGGFFSKSVFLEKANLGMG
                     AHVREGCLLEEEAGGAHSVGLKQTILFPFVTLGSLINFCDCLMAGGTSRSNHSEVGSS
                     YIHFNFTPDADKTTPSLLGDVPRGVMLNQPPIFLGGQGGIIGPVRMGFGNVAAAGSVL
                     RHDYPDDGLLIFETTPADGAKNYRRAAYPNLRRIVENNILYLANLSALESWYAHVRRP
                     FLDARPFGPLIYAGVLETLASAKKERLKRLKAMAQKAAANPGPEEEARKEFLEQTGFC
                     EDLFTGKIPDGFTKTDPLLEAFLQDFEKSAGNDRTDYVGAVRKLPGAVSAKGTAWLNG
                     IVGALCNQAARAMPSMGLFRN"
     gene            1534..>2092
                     /locus_tag="PLR47_10315"
     CDS             1534..>2092
                     /locus_tag="PLR47_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOG13386.1"
                     /translation="MGKLFGTDGIRGEANQYPMTPEIAFGVGRAMAHLFKKNGHRAVI
                     IIGKDTRLSGYMLESSLEAGITSMGGYSYLVGVMPTPAIAFVTQSMRADAGVVISASH
                     NPFQDNGLKIFGGDGYKLSDEQEEFIEDLILNNKLSDLVSRGQDIGKAYRLDGVNGRY
                     IVFAKNTFPRNLSMEGMKIVLDTANG"
BASE COUNT          505 a          582 c          601 g          404 t
ORIGIN      
        1 gggcgccaac attgccggct tcaccaaagt ggccaaagcc atgctggctt acggggtagt
       61 ataataaaaa ggcgataggc tatgggctat gggcgatagg ggaaaaagct cacagctcat
      121 agtaaaaaaa gtggatggta aatagtaaaa aagacaagat gattggcgat gagtgatcgg
      181 cttcactttt cacttttcac tttccactca tcactccaat gggggcctgt atggatttta
      241 agccgagatc ccatgaaaaa atacagcagc tgctggacaa aggggtaaac atccccaatc
      301 cggcgacgct ggacatcggc gacgacgtca atatcgatca gatttcggga aagggcacag
      361 cgatctatcc cggatgccgg atttacggcg ggaaaaccgt catctcgtcc ggctgcaagc
      421 tggggtatga agcgccggcg accatcgagg actgtcagat cggagagcgc gtcgagctga
      481 agggaggctt cttcagcaaa tcggtttttc tggagaaggc aaacctcgga atgggcgcgc
      541 acgtccggga aggatgcctc ctggaggaag aagcgggcgg cgcccactcg gtcggcctga
      601 aacagaccat tctctttccc tttgtcacgc tgggcagcct gatcaacttc tgcgactgcc
      661 tgatggccgg cgggaccagc cgcagcaacc actccgaagt gggcagttcc tacattcact
      721 tcaatttcac gccggacgcg gacaaaacaa ccccttccct cctgggggat gttccccggg
      781 gcgtaatgct caaccagccc cccatttttc tgggcggcca gggcggcatc atcggtccgg
      841 tgcgcatggg attcggcaac gtcgcggccg cgggctcggt cctgcgccat gattatcccg
      901 atgacggcct gttgattttt gagacaacgc ccgcggacgg cgcgaaaaac tatcgtcgcg
      961 ccgcttaccc gaacctgcgg agaatcgtgg aaaacaatat cctgtatctg gccaacctgt
     1021 ccgctctgga atcctggtac gcgcacgtcc gcaggccgtt tttggatgcc cggccgttcg
     1081 gcccgctgat ttacgcgggc gttctggaga cgctggcctc ggccaaaaaa gagcggctga
     1141 agaggctaaa ggcgatggcc caaaaagccg ccgccaatcc cgggccggaa gaggaagcca
     1201 gaaaagagtt tcttgaacaa accggtttct gcgaagacct cttcaccgga aaaataccgg
     1261 acggcttcac gaaaacggat ccgcttctcg aagcctttct gcaagacttc gaaaaaagcg
     1321 cgggaaatga cagaaccgac tacgtcggag cagtccggaa actgcccggg gcggtttctg
     1381 caaaagggac ggcatggtta aacggcatcg tcggggcgct ctgcaatcag gctgcgcggg
     1441 ccatgccgtc gatgggatta ttcaggaact gaaacgggca cagacccggt ttttgctaaa
     1501 tagctgaaac aatgaacatc aaggagacca tttatgggca agttattcgg cactgacgga
     1561 attcgaggag aagcgaatca atatcccatg acccctgaaa tcgcctttgg cgtcgggcgg
     1621 gccatggcgc atttgtttaa aaagaacgga caccgggccg tcatcattat cgggaaagac
     1681 acccggcttt ccggctacat gctggagagc tcgctggaag ccggcatcac gtccatgggg
     1741 ggctattcct acctggtcgg cgtgatgccc acgccggcga tcgccttcgt cacccagagc
     1801 atgcgcgccg acgcgggtgt cgtgatttcc gcgtcccaca atccctttca ggacaacggc
     1861 ctgaagattt tcggcggcga cggctacaag ctttccgacg agcaggagga attcatcgaa
     1921 gacctgatcc tcaacaacaa actttccgac ctggtgtcca ggggtcagga catcggaaag
     1981 gcataccgcc tggacggcgt gaacggacgc tacatcgtct ttgccaaaaa cacctttccc
     2041 cgcaatcttt ccatggaagg catgaaaatc gttctggaca ccgccaacgg cg
//
LOCUS       DAOSIM010000313         2090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_46015_length_2090_cov_7.257985, whole genome shotgun sequence.
ACCESSION   DAOSIM010000313 DAOSIM010000000
VERSION     DAOSIM010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46015_length_2090_cov_7.257985"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..393)
                     /locus_tag="PLR47_10320"
     CDS             complement(1..393)
                     /locus_tag="PLR47_10320"
                     /inference="COORDINATES: protein motif:HMM:NF015166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13387.1"
                     /translation="MLIIIHLLLMLAASLCLLAGVGLAMFGRKRKFWLQGHKNLNSAG
                     GIFLAAGGVMAFVHIILAGADHLAGIHQQVGAIAVMLAFLTVLLGFYSLKAPNKNVFR
                     AVHRWSGRLSLIAILAALTLGLSMIGIF"
     gene            complement(445..>631)
                     /locus_tag="PLR47_10325"
     CDS             complement(445..>631)
                     /locus_tag="PLR47_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG13388.1"
                     /translation="IEQAVKLALDAGVKQLGLFHHNQDRFDTAVDEMVDACRQIIAEK
                     HSPLECFAVYQGMELSL"
     assembly_gap    632..641
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<642..1199)
                     /locus_tag="PLR47_10330"
     CDS             complement(<642..1199)
                     /locus_tag="PLR47_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG13389.1"
                     /translation="MIIRCWGARGSIPVSGRKYLRYGGNTTCLEIRNNRNDILLVDAG
                     SGIREAGNSFLDNGHHDFILLLTHAHWDHIMGFPFFKPLYLRKTKIDVWGCAFAQDSI
                     REMLARVMSAPYFPINFDAIHADVTYADICMEAYELGGMTITPVALNHPNQGNGYQFS
                     ENGKRFVFLTDNELGFRHEGSLSYEN"
     gene            1339..1539
                     /locus_tag="PLR47_10335"
     CDS             1339..1539
                     /locus_tag="PLR47_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOG13390.1"
                     /translation="MKNIQIAGMSCGHCVMAVTKALGAVDGIKDIRVDLKTGVATYDE
                     VKPVEASIVAAAIKKAGYDIVG"
     gene            1529..>2090
                     /locus_tag="PLR47_10340"
     CDS             1529..>2090
                     /locus_tag="PLR47_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOG13391.1"
                     /translation="MSDKVVISVGGMTCAACVRRVENALKNVEGVVDAGVNLATARAT
                     VVHQPRWAGVPALARVVAEQGYEFLGEVSDAAADPIEASRVRELGELKLKVSCGAVLS
                     VLIFFGSMQHWFGFLQAIPRQSMLAAMFILTAPAVFWVGSRFFIGAWKAAKQKTSDMN
                     TLVAVGAFSAYAYSAAATFFPRFFGSA"
BASE COUNT          450 a          528 c          624 g          478 t
ORIGIN      
        1 ttaaaagatt ccgatcatgg agagccccag ggtcagcgcg gcaaggattg cgatcaggga
       61 aaggcggccc gaccagcggt gaacggctcg aaagacgttt ttgttggggg cttttaaaga
      121 atagaatccg agaagcaccg tcagaaatgc cagcatgacg gcgatggccc ccacctgttg
      181 gtggattccc gccagatgat ctgcgcccgc caggatgata tgcacaaagg ccatgacgcc
      241 gcccgcggcc agaaaaatgc ccccggcgct gttgagattt ttgtgtccct gcagccagaa
      301 ttttcttttc cttccgaaca tggccaggcc gacgcctgcc agaaggcata aagacgccgc
      361 aagcatcagc aacagatgaa taatgatcag catgacatga ggtctcctcg cgccggggga
      421 ttcgggtttt ttcttaaaac aagactacag ggacaattcc attccctgat aaacggcaaa
      481 gcattccagg ggtgaatgtt tttcggcgat gatttgccgg caggcgtcga ccatttcgtc
      541 gacggccgtg tcgaagcggt cctgattgtg atggaacagc cccagttgtt tgacgccggc
      601 atccagcgca agtttcaccg cctgttcgat gnnnnnnnnn ngttttcata ggacagcgat
      661 ccttcgtgcc ggaatcccag ttcgttgtcg gtcaggaaaa caaagcgttt gccgttttct
      721 gaaaattgat agccgtttcc ctgattgggg tggttgagcg cgaccggcgt gatggtcatg
      781 ccgcccagtt cgtaggcttc catgcagatg tccgcatagg tgacatcggc atggatggcg
      841 tcgaaattga tggggaagta gggagcggac atgacgcgcg cgagcatttc ccggatggag
      901 tcctgggcaa acgcgcagcc ccagacgtcg atcttggttt tgcgcaggta aagcggtttg
      961 aagaaaggga atcccataat atggtcccag tgggcatgag tcaaaagcag aataaaatcg
     1021 tgatggccgt tgtccagaaa gctgtttcct gcttcgcgga tgccggagcc tgcgtccacc
     1081 agcagaatat cgttgcggtt gttccggatt tccagacagg tcgtgttgcc gccgtaccga
     1141 aggtatttcc gtcctgatac cggtatggaa cctctcgccc cccagcaacg tatgatcata
     1201 aatcatcccc tgttggcata atcatacaga atatttgttt tttttcaagc agggagatca
     1261 taaaaaatgc ggttttgccc ttgctattgg accaataacc attatgataa aaaaaatgag
     1321 aatcatcgga ggtgtcctgt gaaaaacatt caaattgcgg gcatgagttg cgggcactgc
     1381 gtcatggcgg ttacaaaagc gctcggtgcg gttgatggaa ttaaggatat ccgggtcgat
     1441 ttaaaaacag gggtcgcgac gtatgatgag gtgaagcccg tggaagcatc catcgttgct
     1501 gcggccatta agaaggcggg ctacgacatt gtcggataaa gtcgtgatct cggtgggcgg
     1561 catgacctgc gcggcctgtg tgcgccgcgt ggaaaacgcg ctgaaaaacg tcgaaggcgt
     1621 ggtggatgcg ggcgtcaatc tggccaccgc ccgcgccacc gtggtccacc agccccgctg
     1681 ggcgggtgta cccgcgctgg cgcgcgttgt tgccgaacag ggatacgaat tccttggcga
     1741 ggtttccgat gcggccgccg atccgatcga ggcttcccgc gtccgggagc tcggcgagtt
     1801 gaagctgaaa gtctcctgcg gcgccgtgtt gagcgtcttg atctttttcg gatcgatgca
     1861 gcactggttc ggctttttgc aggcgattcc ccgtcagtcg atgctcgcgg caatgttcat
     1921 tttaacggcg cccgcggtgt tttgggtggg gagccggttt ttcatcggag cctggaaggc
     1981 cgcgaagcaa aagacgtcgg acatgaacac gctggtggcg gtgggcgcct tttccgccta
     2041 tgcctattcg gccgccgcca cttttttccc ccgtttcttt ggcagtgcgg
//
LOCUS       DAOSIM010000314         2084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_46195_length_2084_cov_5.264662, whole genome shotgun sequence.
ACCESSION   DAOSIM010000314 DAOSIM010000000
VERSION     DAOSIM010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46195_length_2084_cov_5.264662"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /gene="rpsI"
                     /locus_tag="PLR47_10345"
     CDS             <1..142
                     /gene="rpsI"
                     /locus_tag="PLR47_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOG13392.1"
                     /translation="KALLECDAELRPILKKAGFLTRDARIKERKKYGQPGARKRFQFS
                     KR"
     gene            188..1228
                     /gene="argC"
                     /locus_tag="PLR47_10350"
     CDS             188..1228
                     /gene="argC"
                     /locus_tag="PLR47_10350"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOG13393.1"
                     /translation="MKTKAAIFGASGYTGQELIRILSGHPQVELAAVTSRRYAGVKVS
                     DVFPSLAGLTFLTYTDASPREIAGICDVVFLALPHGVSMEIAPVFLEAGKKVIDLSAD
                     YRLRDPATYEEWYAKHSSAGLFGQAVYGLPELYRDAVKTSSLIANPGCYPTSIILGLA
                     PALKNKLLDVSTIIADSASGVSGAGRDPAVGSLFCEADGGFKAYKVGKHRHTPEIEQE
                     LNKLAGEKFFISFTPHLLPVKRGILSTMYASLRKEATQADLHALYASFYAGEKFIRIC
                     SAGTFPNLSSVCGSNYCDIGLTVDPRTRRVIIISAIDNLVKGAAGQAVQNMNLVCALP
                     EDSGLGAPPLYP"
     gene            1242..>2084
                     /gene="cysS"
                     /locus_tag="PLR47_10355"
     CDS             1242..>2084
                     /gene="cysS"
                     /locus_tag="PLR47_10355"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOG13394.1"
                     /translation="MAQKIYNTKTRKKEIFQPVREGAVGMYVCGITAYDVCHIGHARA
                     AVVFDVVFRHLKARGYDVTYVKNFTDIDDKIIDRANREGVGIAEIAERYIALHDEDMA
                     ALNVLAPTVTPRATGHMEDMIALIRTLEEKGIAYAVDGDVFFAVAKFSAYGGLSGRNL
                     DEMMAGARVDVNDKKRNPLDFVLWKRSKEGEPSWDSPWGMGRPGWHIECSAMSRRYLG
                     EPFDIHGGGEDLIFPHHENEIAQSQAATGVPPAHYWMHNGFVKINAEKMSKSLGNIFP
                     IREIV"
BASE COUNT          468 a          588 c          609 g          419 t
ORIGIN      
        1 caaagcgctt ctggaatgcg acgccgagtt gcgccccatt ctgaaaaaag cgggatttct
       61 gacgcgcgac gcccgcatca aggagcgtaa gaagtacggc cagccgggcg cccgaaagag
      121 attccaattt tccaagcgtt aatcattcaa ggggggcttc gggctcccct tttttttgga
      181 gttttttatg aaaacaaaag cggcgatatt cggcgccagc ggttacaccg gtcaggaact
      241 gatccggatt ctgtccggac acccgcaggt ggaactggcg gccgtcacct caaggcgcta
      301 tgccggcgtg aaggtctcgg acgtttttcc gtcccttgcc ggcctgacgt ttctcacgta
      361 caccgacgct tcgccccggg aaatcgcagg gatttgcgat gtcgtttttc tggcgctgcc
      421 gcacggcgtg tctatggaga tcgcccccgt ctttctcgaa gcgggcaaaa aggtgatcga
      481 tctgtccgcc gactaccgcc tgcgggatcc ggcaacctat gaagagtggt atgccaaaca
      541 ttccagcgcc ggcctgttcg gacaggcggt ttacgggctt ccggaacttt accgggacgc
      601 ggttaaaaca tcgtcgttga ttgccaatcc cggctgttat ccgaccagca ttattctggg
      661 gctcgcgccc gcgctcaaaa ataaactcct ggacgtttcc acgatcattg ccgattcggc
      721 ctccggcgtg agcggcgcgg gaagggaccc ggccgtcgga tcgctcttct gcgaagcgga
      781 tggtggcttt aaggcctaca aggtcgggaa gcaccggcac accccggaaa tcgagcagga
      841 gctcaacaaa ctggccgggg aaaaattctt catttccttc acgccgcacc tgctgccggt
      901 gaaaaggggc attctgagca caatgtacgc gtcgctccga aaagaagcga cccaggcgga
      961 tctgcacgcc ctgtatgcct ccttctacgc cggggaaaaa ttcatccgga tttgttcggc
     1021 gggaacgttt cccaatcttt cttccgtttg cggatccaat tactgcgaca tcggcctgac
     1081 cgtcgatccg cggacacgcc gggtgattat tatttccgcc atcgacaatc tggtcaaggg
     1141 cgccgccggc caggccgtgc agaacatgaa tctggtctgc gccctgccgg aagacagcgg
     1201 tcttggggcg cccccgctgt atccctgaaa aaggcaagaa aatggcccaa aaaatctata
     1261 atacgaagac aagaaaaaaa gaaatcttcc agccggtccg cgaaggcgcg gtcggaatgt
     1321 acgtgtgcgg catcacggcc tacgatgtct gccatatcgg ccacgcccgc gcggcggttg
     1381 tgtttgacgt cgttttccgc catctgaagg cgcgcggcta cgacgtgacg tatgtgaaaa
     1441 atttcaccga catcgacgac aaaatcatcg atcgggcgaa tcgggaaggc gtcggcattg
     1501 ccgagatcgc cgaacgctac atagcgctgc acgatgaaga tatggccgcc ctcaacgtcc
     1561 tcgcgccgac ggtcaccccg agagccacgg ggcacatgga ggacatgatt gcgctgatcc
     1621 ggactctcga agaaaaaggg atcgcctacg cggtggacgg agacgtgttc ttcgcggtcg
     1681 ccaaattttc agcatacggc ggattgtccg gccgaaacct ggatgaaatg atggccggcg
     1741 cccgcgtgga tgtcaacgac aagaaaagaa atccgctgga tttcgtgctc tggaagagaa
     1801 gcaaggaagg cgagccttcc tgggacagtc cgtggggaat gggcaggccg ggatggcata
     1861 tcgagtgttc ggcgatgagc cggcgctacc tgggagagcc ttttgatatt catggaggag
     1921 gcgaagacct gatctttccg catcatgaaa acgaaatcgc gcaatcccag gcggcaaccg
     1981 gagttcctcc ggcccattac tggatgcaca acggttttgt gaagatcaat gcggaaaaaa
     2041 tgtccaaatc cctgggaaac atcttcccga tccgggagat cgtc
//
LOCUS       DAOSIM010000315         2082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_46256_length_2082_cov_6.610755, whole genome shotgun sequence.
ACCESSION   DAOSIM010000315 DAOSIM010000000
VERSION     DAOSIM010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46256_length_2082_cov_6.610755"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="PLR47_10360"
     CDS             complement(<1..940)
                     /locus_tag="PLR47_10360"
                     /inference="COORDINATES: protein motif:HMM:NF036723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG13395.1"
                     /translation="MSLADRNNPYSFNEFLAWRQGADYYADDPFIQKVVRHYTGDIRR
                     EVDAEARAVSAKASFRWRDMAERIARPEERPYILHYDGHHNRIDRIVRPRDTEIMEKE
                     IFSEAFFAEKTPPWVKLIKMYIIYQNGEACIGCPVTCTEGLVELLNKYADSPETKEIL
                     RHCKEGIDGDFAIGAQYLSEIQGGSDVPSNVLEAVRENGVWKLYGTKFFCSATHADYA
                     VVTAKPAGSEKVGLFVVPSWLAGSKEKEIRNGCTIDRIKWKMGTSELTTAELTFNGAV
                     AYPVGPLDKGVANVVGIVLTYSRLTVGLSAAAYMTRA"
     gene            complement(1082..1357)
                     /locus_tag="PLR47_10365"
     CDS             complement(1082..1357)
                     /locus_tag="PLR47_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOG13396.1"
                     /translation="MKRMHVLISGRVQRVFFRAETLRTALSLGLAGWVRNMEDGRVEA
                     LFEGEDESVDRMLAWCRQGPLHARVDRVDMTEEPCRGDLQGFRIKDF"
     gene            complement(1404..>2082)
                     /locus_tag="PLR47_10370"
     CDS             complement(1404..>2082)
                     /locus_tag="PLR47_10370"
                     /inference="COORDINATES: protein motif:HMM:NF012743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOG13397.1"
                     /translation="MDIEGLGEKLSAQLFDAKMIADPADLYFLTKEKLLGLDRQAEKS
                     AENLLEAVERSKTPALDKFIFALGIRHVGERTAKLLAGHFGSLEALMSAGCEELTAIR
                     EIGPEIAQSILEFFAEPKNRIVLEKFQRAGLSPRTPAPTAQASLQGKSFVFTGSMESM
                     GRNKAKAAVESLGGIVHSSVTGKTSYVVAGSDPGSKLEKARKQSIPVIGEEEFLTMIG
                     RQIHRQA"
BASE COUNT          395 a          617 c          580 g          490 t
ORIGIN      
        1 cggcccgcgt catgtaagcc gcggcggaaa gccccaccgt caggcgcgaa taggtgagca
       61 cgatgcccac cacgttggcc actcccttgt ccagcggccc gacggggtaa gccaccgcgc
      121 cgttgaaggt gagttcggcc gtcgtcagtt cgctggtgcc cattttccac ttgatccggt
      181 cgatggtgca gccgttgcgg atttcctttt ccttgctgcc cgcgagccac gacggcacga
      241 caaacagccc caccttttcc gagcctgccg gtttggccgt cacgacagca taatccgcat
      301 gcgtggccga gcagaagaat tttgttccat agagtttcca gacgccgttt tccctcacgg
      361 cttccagaac attggacggc acgtccgatc cgccctggat ttcggacaga tactgcgcgc
      421 cgatggcaaa gtcgccgtcg atgccttcct tgcagtgccg aaggatttct tttgtttccg
      481 gagaatcggc gtacttgttg agcagttcga cgaggccttc cgtgcaggtg acggggcagc
      541 cgatgcaggc ctcgccgttc tggtaaatga tatacatctt gatcagtttg acccagggag
      601 gtgttttctc cgcaaagaac gcttcggaaa aaatttcttt ttccatgatt tccgtatcgc
      661 ggggccgcac gatccggtcg atgcggttgt ggtggccgtc gtagtgcaga atataggggc
      721 gctcctccgg ccgtgcgatc cgctcggcca tgtcgcgcca gcggaaagag gctttggcgg
      781 agacggcgcg cgcttcggca tccacctcgc gccggatatc gccggtatag tggcgcacga
      841 ctttttgaat gaaaggatca tcggcgtagt aatccgcacc ctggcgccag gccaggaatt
      901 cattgaagct gtaagggttg tttcgatctg ccaacgacat gaaaatttcc tcctgttctt
      961 tttgtgcaaa gcgggttgcc cgtccaaggg cggtcaggaa cgtgtcctgc gactgctata
     1021 tagaaccgga gtttgcttcg tcaagtcttt tcaggttcag ttttctgcaa ccggtcgcgg
     1081 gttaaaaatc tttgatacgg aagccttgaa gatcgcctcg acagggttcc tccgtcatgt
     1141 ccacccggtc cacgcgggcg tgcaacggtc cctgacggca ccacgccagc attcggtcca
     1201 cgctttcgtc ttctccctca aaaagggctt cgacgcgtcc gtcttccatg ttgcgcaccc
     1261 atcccgcaag gccgaggcta agcgcggttc gcagcgtttc cgcacgaaag aaaaccctct
     1321 gcacacgccc tgaaatcaaa acatgcattc ttttcataac caccattctg aggggcggcg
     1381 gcccggaaag gatgaacaat ctttcacgcc tgccggtgga tttgcctgcc gatcatggtt
     1441 aaaaattctt cttcgccgat gacgggaatg ctttgctttc tggccttttc aagttttgat
     1501 cccggatcgc tccccgcgac cacatacgag gttttgcccg tgacggagga gtggacgatg
     1561 ccgcccaagg actcaacggc ggccttggcc ttatttcttc ccatgctttc catggagcct
     1621 gtaaaaacaa acgatttgcc ctgcagactc gcctgggccg tgggggccgg ggttcggggg
     1681 gacaggccgg cccgctgaaa cttttccaaa acgatcctgt tttttggctc cgcgaaaaat
     1741 tccagtatgc tctgggcgat ttccgggccg atttcgcgga tggccgtcaa ttcttcgcaa
     1801 ccggcgctca tcagtgcctc caggctgccg aaatgtccgg ccagaagttt ggcggtgcgt
     1861 tcgccgacgt ggcgtatgcc gagcgcgaag ataaacttgt ccagcgcggg ggtcttggag
     1921 cgttctacgg cttcgaggag attctccgcg gatttttccg cctgacgatc cagccccagc
     1981 agcttctcct tcgtcaagaa atacaggtcc gccggatcgg cgatcatctt cgcgtcaaaa
     2041 agctgcgcgg acaatttttc tcccaggcct tcaatatcca tc
//
LOCUS       DAOSIM010000316         2080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_46303_length_2080_cov_8.837037, whole genome shotgun sequence.
ACCESSION   DAOSIM010000316 DAOSIM010000000
VERSION     DAOSIM010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46303_length_2080_cov_8.837037"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..396)
                     /gene="rplL"
                     /locus_tag="PLR47_10375"
     CDS             complement(7..396)
                     /gene="rplL"
                     /locus_tag="PLR47_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOG13398.1"
                     /translation="MADQITKEDVIKFIENMTVLELSALVKELEEKFGVSAAAPMMAA
                     AAMPGQAAAAPAAEEKTEFNAILTGFGAEKIQVIKVVRAITGLGLKEAKDLVEGVPKP
                     VKEGVSKEEAADIKKKIEEVGGTVEIK"
     gene            complement(422..949)
                     /gene="rplJ"
                     /locus_tag="PLR47_10380"
     CDS             complement(422..949)
                     /gene="rplJ"
                     /locus_tag="PLR47_10380"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOG13399.1"
                     /translation="MDRKAKEQIVSELQEKLKKAHLGVLTRFNAMNVEKMEALRNALR
                     KNDAELKVVKNTLLGIASRETGFSILADHFKYPVAVVLSYQDPVAPTKALVDFAKKNP
                     ELDIKVGVLDGKMLTREDIAALADLPSREVLLGKLVSVMAAVPTSLVTVLSAVPRGFV
                     QALHAYSEKKQTLNS"
     gene            complement(1224..1925)
                     /gene="rplA"
                     /locus_tag="PLR47_10385"
     CDS             complement(1224..1925)
                     /gene="rplA"
                     /locus_tag="PLR47_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOG13400.1"
                     /translation="MVRRGKRIIAAKQKVEPTRRYTLKEATEMVVSMAGVKFDETVDA
                     AVRLGVNPAHADQMVRGSVVLPNGLGKTVRVLVFAKGEKEKEALDAGADLVGNEEIIE
                     KIKGGWLEFDRVIATPDMMGSVGKIAKILGPRGLMPNPKVGTVTFDIANAVRELKAGK
                     VEFRVEKAGIVHSPVGKVSFGAEKLHENIMALLETIIKLKPASSKGTYIRSIAVSSTM
                     GAGVKIDPLDIKSAG"
     gene            complement(1945..>2080)
                     /locus_tag="PLR47_10390"
     CDS             complement(1945..>2080)
                     /locus_tag="PLR47_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOG13401.1"
                     /translation="GTVTAKQVQDIAQLKFNDLNAVDLEGAVRIIAGTARSMGIEIAG
                     "
BASE COUNT          367 a          660 c          510 g          543 t
ORIGIN      
        1 attaccttat ttaatctcga ccgttccgcc gacttcctcg atcttcttct tgatatcggc
       61 ggcttcttct ttggaaacgc cctctttcac cggtttgggc acgccttcca ccagatcctt
      121 ggcttctttc aggccgagcc cggtgatggc gcgaacgacc ttgatgacct gaatcttctc
      181 cgcgccgaat cccgtcagga tggcgttgaa ctcggtcttc tcttccgccg cgggcgcggc
      241 ggcggcctgt ccgggcatcg cggcggcggc catcatcgga gcggcggcgg acacgccgaa
      301 tttttcttcc agttccttca ccagagcgga aagctccagc acggtcatat tttcaataaa
      361 tttaatgacg tcttctttcg taatctgatc agccatggtt ctattcctct ttcgtgtgtt
      421 tttatgagtt taaagtttgc tttttctcgc tgtaggcgtg gagcgcctgc acaaagcctc
      481 tggggaccgc gctcaaaacc gtgaccagcg acgtcggcac cgccgccatg acggaaacca
      541 gcttgcccag aagaacttcc cggctgggca aatcagccag cgcggcgatg tcttcccggg
      601 tcagcatttt gccgtccagc acaccgactt tgatatccag ttcgggattc ttcttggcaa
      661 aatccacgag cgcctttgtc ggcgccaccg gatcctgata gctcaagaca accgcgacgg
      721 gatacttgaa gtgatccgcc agaatggaaa agccggtttc tctcgacgcg atgcccagca
      781 gggtgttttt gacgaccttc agctccgcat cgtttttgcg caatgcgttc cgaagggctt
      841 ccattttttc aacgttcatg gcgttgaaac gggtcaaaac gcccaagtgg gcctttttga
      901 gtttctcctg aagctcggat acgatctgtt cctttgcttt tcgatccaat tgatcagcct
      961 cctttcttga tgattttcaa cctgaggccg aacacccagc cgcacacgga atgaaaaacg
     1021 gccgatcctg tctttccgat ccccgttttc ggcaaataaa gattccctcg aagtccatcc
     1081 acgaaattct tttttgcttt gcgagtctcg gcaggcccct gtccgtttaa acttccgtac
     1141 ctgctgtctt cgacctgatg ctgcttatcc ctcttgcgcg gacgccgcgc cttttaccgc
     1201 acggcttccg cgaattcttt ccttcagccc gcgctcttga tatccagcgg atcgatcttc
     1261 acccccgcgc ccatcgtgct ggacaccgcg atgctcctga tataggtccc cttgctcgac
     1321 gcgggtttca gcttgatgat ggtctccagg agcgccatga tgttttcgtg aagtttttcg
     1381 gcgccgaagg ataccttgcc caccggcgag tgcacgatgc cggccttttc cacgcggaat
     1441 tccaccttgc ccgccttcag ctcccgaacc gcgttggcga tgtcaaaggt gaccgttccc
     1501 accttgggat tgggcatcag gccgcgcggg ccaagaatct tggcgatttt ccccacggac
     1561 cccatcatgt ccggcgtggc aatcacgcgg tcgaactcca gccagccgcc tttgatcttc
     1621 tcgatgattt cctcgttgcc gaccagatcc gctccggcgt ccagggcctc tttttccttc
     1681 tcgcccttgg caaaaaccag cacgcggacg gtttttccca gaccgttggg gagcacaacg
     1741 ctgccccgaa ccatttgatc ggcatgcgcc gggttgacgc ccaggcggac ggcggcatcc
     1801 accgtctcat caaacttgac tcccgccatg gacacgacca tttccgtagc ctctttcagc
     1861 gtgtagcgcc gggtcggctc gaccttttgc tttgccgcga taatgcgttt gcctcgtctt
     1921 accatgatcc aacctcgtcc ttcgttaacc tgctatttct attcccatgg accttgccgt
     1981 gccggcaatg atcctgaccg cgccttcgag atcaacggcg ttcaggtcat tgaatttcag
     2041 ctgcgcaatg tcctgaacct gtttggccgt gacggttccc
//
LOCUS       DAOSIM010000317         2075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_46462_length_2075_cov_6.442079, whole genome shotgun sequence.
ACCESSION   DAOSIM010000317 DAOSIM010000000
VERSION     DAOSIM010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46462_length_2075_cov_6.442079"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            322..>2075
                     /gene="fdnG"
                     /locus_tag="PLR47_10395"
     CDS             322..>2075
                     /gene="fdnG"
                     /locus_tag="PLR47_10395"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014258623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:907..909,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HOG13402.1"
                     /translation="MQITRRGFLTMTGAIGGGVALSSLGLNLNPTRAYADELGKMDRI
                     KIAKQVTTICCYCSVGCGLVCSVDKATGKIFNIEGDADHPINEGSLCAKGAGFFDLTE
                     ANKHRLTKVLYRKPYGTEWEEKDWDFALSRIARLVKDTRDKNFTKRNDKGELVNRCEA
                     IGHYGSSNVDNEECWIMSVKSRALGMVYIDHQARVUHSPSVAALGESFGRGSMTNHYC
                     DLKNADVILIMGSNAAEHHPIAFKWILRAKEKGATIIHVDPRYTRTSARCDFHVPLRS
                     GTDIAFLGGMIHYILENKKFFKDYVLNYTNASFIVDNGFNFKDGLFSGYDPKKRKYDK
                     ATWVFDKDGKGIPQRDMSLTHPRSVFQLLKKHYSRYTLDKVSSITGVSKENLLKVYEI
                     YASTGVPDKAGTECYALGWTHHTTGSQNIRTMSIIQLLLGNMGIAGGGINALRGEPNV
                     QGSTDHCILYGNLPGYLKMLSASLDTLDKYLHKYTPESKDPQSANYYSNYPKFFVSFL
                     KSLWEDKATKENAFGYSWLPKMDDGKHYSTMHMFDAMYAGKVKGYFCVGADTAVSTPN
                     SNKVRKAMENLDWLVGEN"
BASE COUNT          535 a          521 c          538 g          481 t
ORIGIN      
        1 ctttttggcc gcggcattga ggctgcgctg ctcatcgatg gccttgagaa tgtcatcccg
       61 tcccttgccg aagacgactt ttccgttttt ttcaatccaa accttgtatt tgatttccat
      121 gggaagaact cctcctgcct gcaggactcg cgaatgtcta ttgtataggg aagttgggcg
      181 ggaaatgcaa tcaaatcatt atcccgccgt ccggtttctg atgtatgttt gacaaaacat
      241 attgactttt gcatgacttt cggtcaacat atgccatgta tgacatagat caaaaaagac
      301 acatgcatcg ggaggatttt tatgcaaatt acaagaagag ggtttttaac catgaccggc
      361 gccatcggag gcggggttgc cctgtcgagc ctggggctga acctcaatcc gacccgggcc
      421 tatgccgatg agcttggcaa aatggatcgg atcaaaatag ccaaacaggt tacgacgatc
      481 tgctgttact gctccgtcgg ctgcggtctg gtctgcagcg tggacaaagc gacagggaaa
      541 atcttcaaca tcgaaggcga cgcggatcat cccatcaacg aggggtcgct ttgcgccaag
      601 ggcgcgggct tcttcgatct gacggaagcc aacaaacacc gcctgaccaa agtgctttac
      661 cgcaaacctt acgggacgga atgggaagaa aaagactggg actttgcgct ttcccggatt
      721 gcccgtctgg tcaaggacac gcgtgataag aattttacaa agagaaatga caagggcgaa
      781 ctggtcaacc gctgcgaggc gatcggccac tacggcagtt ccaacgtgga caacgaagag
      841 tgctggatca tgagcgtcaa gtcccgcgcg ctgggtatgg tttatataga ccatcaggcc
      901 agggtctgac acagtccctc tgtagcggct ctgggagagt cgttcggacg cggttccatg
      961 acgaaccatt actgtgattt gaagaatgcc gatgttatcc tgattatggg cagcaatgcc
     1021 gccgagcatc accccatcgc ctttaagtgg attttgagag cgaaagaaaa aggcgcaaca
     1081 atcattcacg ttgatccccg ctacacaaga acgtccgccc gctgcgattt ccatgtgccg
     1141 cttcgttccg gcacggacat cgccttcctg ggcggcatga tccattacat tctggaaaac
     1201 aagaagttct tcaaggatta tgtcctcaat tacaccaacg cgtcgttcat cgtggacaac
     1261 ggctttaatt tcaaggacgg tttgttttcc ggttatgatc ccaagaaaag aaagtacgat
     1321 aaagccacat gggtcttcga taaagacgga aaaggcattc cccagaggga tatgagcctt
     1381 acgcatccgc gctcagtgtt tcaattgctc aaaaagcatt attcgcgtta cacgctggat
     1441 aaagtttcca gcatcacggg cgtgtccaaa gaaaacctgc ttaaggttta tgaaatctac
     1501 gcttccaccg gcgtgccgga caaggcggga acggaatgct acgctctggg ctggactcac
     1561 cataccaccg gcagccagaa catccgcacg atgtcgatta ttcagctgct gctgggcaat
     1621 atgggcatcg cgggcggcgg catcaatgcg cttcgcggcg aacccaacgt tcagggttcg
     1681 accgaccatt gcattttata cggcaatctg cccggctatc tgaagatgct ttccgcgtcg
     1741 ctggatacat tggacaaata tctgcacaaa tacacgccgg aatcgaaaga tccccagtcg
     1801 gccaactatt attcgaacta tccgaagttt tttgtcagtt tcctgaaatc tctttgggaa
     1861 gacaaggcga caaaggaaaa tgcattcggt tactcctggc tgcccaaaat ggacgacggg
     1921 aaacactatt ccaccatgca catgttcgac gcgatgtatg ccggaaaggt gaagggatat
     1981 ttctgcgttg gcgcggatac ggcggtgagc acaccgaatt ccaataaagt ccgcaaggcg
     2041 atggaaaatc tggactggct ggtcggtgaa aatat
//
LOCUS       DAOSIM010000318         2064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_46786_length_2064_cov_5.598805, whole genome shotgun sequence.
ACCESSION   DAOSIM010000318 DAOSIM010000000
VERSION     DAOSIM010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46786_length_2064_cov_5.598805"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..943
                     /locus_tag="PLR47_10400"
     CDS             266..943
                     /locus_tag="PLR47_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13403.1"
                     /translation="MRSECPALFSFADHVDLIAFFHDPMENLDRKNDILLELIARYRH
                     ENRQPDFSPLFIVIFSPALTNLYAAARKRCPHMDRADLIQDICLLFIQTIGNIKLTPD
                     RMAGRIIGELRNRVRTLLNMNPDENLIALDGDGHGDFVIPSCAAETNTDEEIDSDGIV
                     VEISLFLDRLMRRGKITRKDKRIIMDTITEGKSLKDIALPRDYDRLKHRRRQVIEFIR
                     KKYSASS"
     gene            1032..1283
                     /locus_tag="PLR47_10405"
     CDS             1032..1283
                     /locus_tag="PLR47_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13404.1"
                     /translation="MKTEQKNTTCIIRDTFIDMALCMEFLFVIHGANRLLEEDIVRCM
                     EEKYEKAITNLKKSELGNPKIKRETPVDRFVKKLEKETI"
     gene            1332..>2064
                     /locus_tag="PLR47_10410"
     CDS             1332..>2064
                     /locus_tag="PLR47_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOG13405.1"
                     /translation="MQTQEIVNSLGPGAAVFPLARTDDNPFKRNEAGFYLSKTRMSRY
                     TACPRSYRLAYQLNVKPIKTPVELLIGKSTHRLIAESFLARAANMIPELTGSLDAFWT
                     DEMQAPETRAEMEQAKRESGRYACLFIAEVDLKPLAVEKEFSIPVVNYENGDILPVPL
                     VGIIDLVDRPNGSASSPQGGTPRALEIKTRSRKGDDLQPRVSLELTCYAYWIKSQLLS
                     ELNEDVNVDKIPVGYLNIIKTKTPSI"
BASE COUNT          540 a          544 c          523 g          457 t
ORIGIN      
        1 gaaacgaaca acattcttgc cgaaagttcc tgtccctgcg ggcggaaaat aaccaaggtg
       61 gtcggatacc tggtcaccat ccaatgcacg aagtgcaaga cgttcattaa cttttagagg
      121 cccccgaggc ccgtaaaatc aatcattgta aaggaggagg ctccgaggct cacgaagccc
      181 atgccgctgt tgacgcatgg gtttttcttt tgccggttac cggaaagagc tttcttcaaa
      241 gggatttatc caagcgtatg agggtatgcg gtcggaatgc ccggccttgt tttcctttgc
      301 cgaccatgtc gatctgatcg ctttctttca tgacccgatg gaaaatctgg acaggaaaaa
      361 cgacatcctt cttgaactga ttgcccgtta tcgccatgag aaccggcagc cggatttttc
      421 tcctcttttc attgttattt tttctcccgc tttgaccaac ctttatgccg cagccaggaa
      481 aagatgcccg catatggaca gggcggacct cattcaggac atctgcctgc ttttcatcca
      541 gacgatcggc aatatcaaac tcacgcctga caggatggcc ggccggatca tcggagagtt
      601 aagaaatcgg gtcaggacat tgctgaacat gaatcccgat gaaaatctga tcgcccttga
      661 tggtgacgga catggtgatt ttgtaatccc gtcctgtgcc gcggaaacga atacagacga
      721 agagatcgac agtgacggta tcgttgtcga gatttctctc ttcctcgatc gtctgatgcg
      781 cagaggaaag atcacccgga aagacaaacg catcatcatg gatacgatca cggaaggaaa
      841 atcacttaaa gacattgctc ttccgcgcga ctacgaccgc ctgaagcacc ggcgacggca
      901 ggtgatcgag ttcatcagaa aaaaatattc cgcaagtagc tgattttata tggacaataa
      961 agatattttg gcccccggac gctctttttt gaccttatat aagtaggaga ccttttttga
     1021 ggagactgtt gatgaagacc gaacagaaaa acacaacctg catcatccgc gacaccttta
     1081 tagacatggc cctgtgcatg gagttcctgt ttgtcatcca cggcgcgaat cgcttgctgg
     1141 aggaagacat cgtgcgctgc atggaggaaa agtacgaaaa agccataaca aacctgaaga
     1201 aaagcgagct tggcaacccg aagatcaagc gggagacccc tgtggatcgc tttgtcaaaa
     1261 aactggagaa ggaaaccatc tgatcgttgc tgtccggggg cgaatttctt ttttgaaagg
     1321 aggaaatgtt tatgcaaaca caggaaatcg taaatagttt gggaccaggg gccgcggtat
     1381 ttccactggc gcgaacggat gacaatcctt ttaagagaaa cgaggcgggg ttttacctct
     1441 ccaaaacccg gatgtcccgc tacacagcct gcccccgcag ttaccggctg gcctatcaac
     1501 tgaatgtcaa acccatcaag acgccggttg agctcctgat cggcaagtct actcacaggc
     1561 tcatcgccga atccttcctg gcgcgggcgg ccaacatgat tcccgaactg accggctcac
     1621 tggatgcttt ctggacggat gagatgcagg cgccggaaac gcgcgccgaa atggaacagg
     1681 cgaaacgcga gtccgggcgc tacgcgtgcc tgttcatcgc ggaagtcgat ctgaagcccc
     1741 tggccgtcga aaaggaattt tccatccccg tcgtcaatta tgaaaacggc gacatcctgc
     1801 ccgtgccgct ggtcgggatc attgacctcg tggaccgacc gaatggttcg gcaagctcac
     1861 cacaaggagg cacgccgcgc gccctggaga tcaagacgcg ctcccgcaaa ggggatgacc
     1921 ttcaacccag ggtgtccctg gagctcacct gttacgccta ctggatcaag tcccagcttt
     1981 taagcgaact caacgaagat gtgaatgtcg ataaaatccc cgtgggctac ctgaacatca
     2041 tcaaaacaaa aaccccgtcc atcc
//
LOCUS       DAOSIM010000319         2059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_46948_length_2059_cov_7.965569, whole genome shotgun sequence.
ACCESSION   DAOSIM010000319 DAOSIM010000000
VERSION     DAOSIM010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46948_length_2059_cov_7.965569"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PLR47_10415"
     CDS             complement(<1..651)
                     /locus_tag="PLR47_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13406.1"
                     /translation="MILPLCSSALAGGMFGPLQTMSKKTGGVNTAVGCRYAEDTLKGD
                     MDYGIRQHQVYSQAAYGASGRWEIYGRIGISDLQISDAFRSSSALTTVSRDSFEENWK
                     FFGTLGAKAYYPVHSVFGVGAFVQGSCNFSNFTDEVSGTSGGIPFVTDIKVKYLWDVN
                     FGIGLQATVPYGIQLYAGPFVYYTEADAYVPAAVPGIQSGAEKTSLKNASAVGGFFG"
     gene            complement(708..1535)
                     /locus_tag="PLR47_10420"
     CDS             complement(708..1535)
                     /locus_tag="PLR47_10420"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HOG13407.1"
                     /translation="MDYALAKAPSAGADATVRGEEALPGRADDAFQDEYKDDENQDYD
                     DEPFAEERVTIADPIEPFNRAMHRFNDRLYFWLLKPVARGYKAVVPEPARISVKNFYY
                     HLLFPIRFVNCLLQADLTGAGSEAGRFAINTVWGIGGLLDPSSNRGIELQKQNTDFGQ
                     TLGLYGVGHGFYIVWPFLGPSSPRDSVDIAGEYVLDPLSYFTPWYTSLGKRPFKMINN
                     ASLSLGDYEAVIEAAIDPYIAIRNGYIQYRMKEVQTRRARSLFFRNWKSEATRAPAN"
     gene            complement(1711..>2059)
                     /locus_tag="PLR47_10425"
     CDS             complement(1711..>2059)
                     /locus_tag="PLR47_10425"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG13408.1"
                     /translation="FTPEQRREFVDLFEQVLEKTYLDRILEYSNEKIVFYKESMIADD
                     KAEIQSRVITSSKEVPIYYRMILKNGKWKVYDVVVENVSLVQNYRTQFNEILAGGTPE
                     QLLETLRKKVKTH"
BASE COUNT          472 a          595 c          534 g          458 t
ORIGIN      
        1 gccgaaaaac ccgccgaccg cggatgcatt cttcaaagac gtcttttcgg cgcccgactg
       61 aatccccggg acggcggcgg gtacataagc atccgcttcc gtataataaa caaaaggccc
      121 ggcatacagt tgaattccat aaggaaccgt cgcctgaagg ccgatgccga aattgacgtc
      181 ccagagatat tttaccttta tatcagtcac aaaaggaatt ccgccgctgg ttccgctaac
      241 ctcatcggtg aaattgctga agttgcagct tccctgcaca aaagcgccga cgccgaatac
      301 ggaatgaacc ggataatacg ccttggcgcc cagcgtcccg aaaaatttcc agttttcttc
      361 aaaagagtcc cgggaaaccg tggtcagggc cgaagaagag cgaaacgcgt ccgatatctg
      421 caaatcggaa atgccgatgc gcccgtaaat ctcccagcgg ccggaggcac cgtacgccgc
      481 ctgggaatag acctggtgct ggcgaatccc ataatccatg tcgcctttca atgtatcttc
      541 cgcataccgg cagccgacag cggtgttcac gccgccggtt tttttggaca tcgtctgcag
      601 cggcccgaac attccgcccg caagcgcgga agagcaaagg ggaagaatca tcagggctat
      661 cgtgaaaaga atgatttttc tcgtagggtt tctcctttta tgcgttctca gttggccggc
      721 gcccgggtcg cctcactttt ccaattgcgg aaaaagagcg atcttgccct gcgcgtctgg
      781 acttccttca tgcgatactg aatgtagccg ttgcgaatgg caatgtaagg gtcgatggcc
      841 gcttcgatca ccgcttcgta atccccgagg ctcaacgagg cgttgttgat cattttgaag
      901 ggtcttttgc ccaggctggt gtaccagggg gtaaagtagg aaagcggatc cagaacatac
      961 tcgcccgcga tatccacgct gtcccggggg cttgacggac cgagaaacgg ccagacgatg
     1021 taaaagccgt ggcccacgcc gtacagaccc agcgtctgcc cgaaatcggt gttctgtttt
     1081 tgcagttcga tgccccgatt ggaggaggga tccagaagtc ccccgatgcc ccacaccgtg
     1141 ttgatcgcaa aacggcccgc ttccgaaccg gcgccggtca aatcggcctg cagcaggcaa
     1201 ttgacgaagc ggatgggaaa cagcagatgg taatagaaat ttttaacgct gatccgcgcc
     1261 ggttccggaa ccaccgcttt gtacccccgg gcaaccggtt tgagcagcca gaaatacagc
     1321 cggtcgttga accggtgcat ggcgcggttg aaaggttcga tcggatcggc gatggtaacg
     1381 cgctcttccg caaaaggctc gtcgtcgtag tcctgattct catcgtcttt gtattcatcc
     1441 tgaaaagcgt cgtctgcacg gcccggcagc gcctcctccc cgcggacagt cgcatccgcc
     1501 ccggccgacg gagccttcgc gagggcgtaa tccatgcttt tcccattgcc cattacccat
     1561 cgcccatcgc ctactacccc ttgcccatca ctcatcgccg gcgcttccgc gcacgcgata
     1621 ttcaggacca gacagcccgg gaagctcccg gccgcgaaaa aaaggactgt caggagaaaa
     1681 acggacagat tatgcttcat gatcctaata ttaatgggtt ttcacctttt tgcgcagggt
     1741 ttccaggagc tgctccggcg tgccgccagc cagaatctca ttgaactggg ttcggtagtt
     1801 ctgcaccagg ctcacatttt ccacgacgac atcgtaaact ttccacttgc cgttttttaa
     1861 aatcatccgg tagtaaatgg gaacttcctt ggaagaggtg atcaccctgg actgaatctc
     1921 cgccttgtcg tccgcgatca tcgattcctt gtaaaaaaca attttctcgt tggaatattc
     1981 gagaattctg tccaggtagg tcttttccag cacctgttca aaaagatcga caaattctct
     2041 gcgctgctcc ggggtaaac
//
LOCUS       DAOSIM010000320         2024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_48047_length_2024_cov_8.698324, whole genome shotgun sequence.
ACCESSION   DAOSIM010000320 DAOSIM010000000
VERSION     DAOSIM010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48047_length_2024_cov_8.698324"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PLR47_10430"
     CDS             <1..268
                     /locus_tag="PLR47_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOG13409.1"
                     /translation="GVDGLLHISKLGRGKKIKHAQDVLAKDRDIDVKIEKIDREHKKI
                     ALDLASGGAENGGPREESGDDYKSYLPKSPKTMGTLGDLMKKKK"
     gene            complement(391..>2024)
                     /locus_tag="PLR47_10435"
     CDS             complement(391..>2024)
                     /locus_tag="PLR47_10435"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOG13410.1"
                     /translation="LRKNGIRYSKAISLGNEADIDLVDALEYLGEDEQTRAVILYIEG
                     IRDGRRFVEVARRITPRKPVVAQYVGGSASGARAGASHTGAMSGPDFLYDGIFKQAGI
                     LRVPTIEDLYAHGWTLATQPPLRGRRVGVVTNSGGPGTGIAYMCDHEGLEVPRFSESL
                     QRDIRPLIEAHASAANPVDLTFDLGMRKLAVTLPEMIMKSGEVDALILHGIMHSGFMV
                     EIFEHLRDYLQGVSYEAFLKGTRSVKEDAFQLPGKYPFPFLISSFFDDTDDCTRGYLD
                     AGVPVFRSPENAARALGSLHRCRLIRERVLSNAPPLSQAQPAALEILGRAKAEGRGAL
                     DECEAKQVLAAYGVPVTREAKAVTCDDAVEAAKQIRYPVAVKACSWEILHKSGRGLIA
                     LNLKKEAQVQEAFDGIQKAAGKPTPVLVQEMVFGSREFLVGMTRFPGFAPCVVFGLGG
                     VWTEIHRDMTLRQSPLSDSDAAEMLADLRSSRLLGEYRGMPEVKRGALAGILQAVGTI
                     ALLHPEIAEIDINPVMIRGDEPVAADALIGLKAED"
BASE COUNT          420 a          650 c          579 g          375 t
ORIGIN      
        1 cggagtcgat gggcttttgc acatctccaa actgggccgg ggcaaaaaga tcaagcacgc
       61 ccaggacgtg ctggccaagg accgcgacat cgacgtgaag attgaaaaga ttgaccgcga
      121 gcacaaaaaa atcgcgcttg atctggcttc cggcggcgcg gaaaacggcg gcccacggga
      181 agaatcggga gacgattaca agagctacct gcccaagtcg cccaaaacca tggggacgct
      241 gggagatttg atgaagaaaa agaagtaaat aagcaatgag caatgagctt ttttcctatc
      301 gcctatagcc cattgcctct cgcctttctt caccatccgc tatccaccat ccactattca
      361 caaaacgata gcgcactgtc cctcgtacct tcagtcttca gcctttaatc cgatcagcgc
      421 atccgccgca accggttcat cgcctcgaat catgacggga ttgatgtcga tctcggcgat
      481 ctccgggtgc agaagcgcga tggtccccac cgcctgaaga atcccggcca gggccccgcg
      541 cttcacttcc ggcatgcccc ggtattcgcc cagcaggcgc gagctgcgca ggtcggccag
      601 catttcggcc gcgtccgaat cggacagagg cgactgccgc aaagtcatgt cgcggtggat
      661 ttccgtccag acaccgccca gaccgaaaac cacgcaaggc gcaaatccgg ggaaccgcgt
      721 catgccgacg agaaattcac ggctgccgaa gaccatttcc tgcaccagca ccggcgtggg
      781 cttccccgcc gccttttgaa tgccgtcaaa cgcttcctga acctgcgcct cttttttgag
      841 attcagcgca ataaggcccc tgccgctttt gtgcaggatt tcccaactgc acgccttgac
      901 ggctacggga taccgaattt gcttggccgc ttccacggcg tcatcgcagg tcacggcctt
      961 cgcctcgcgg gtaacgggca cgccgtaggc cgccagcacc tgcttggcct cgcattcatc
     1021 cagcgcgccg cgcccttcgg ctttcgcccg tcccagaatt tccagcgcgg cgggctgtgc
     1081 ctgggacagc ggcggcgcat tcgaaagaac tctctcccga atcagccggc agcggtgcag
     1141 ggaaccgagc gcgcgggcgg cattttccgg agagcggaac accggaacgc cggcgtccag
     1201 atagccgcgc gtgcagtcgt cggtgtcgtc gaagaacgag gagatcaaaa acggaaacgg
     1261 gtatttgccc ggcagctgaa atgcgtcttc cttgacggat ctcgtacctt tcagaaaagc
     1321 ctcgtaggat acaccctgaa gataatcgcg caggtgttcg aaaatttcga ccatgaagcc
     1381 cgagtgcatg atgccgtgca gaatcagcgc gtccacctcg ccgcttttca taatcatctc
     1441 cggcagcgtt acggccagct ttcgcatgcc gaggtcaaac gtcaggtcca ccgggttggc
     1501 ggccgaggcg tgcgcttcga tcagcggccg gatgtctcgc tggaggctct cggagaagcg
     1561 aggaacctcc agcccttcat ggtcgcacat gtaggcaatg cccgtaccgg gtccgccgga
     1621 attggtcaca acgccaaccc gccttccgcg aaggggcggc tgcgtggcaa gcgtccagcc
     1681 gtgcgcgtag agatcctcga tcgtcgggac gcgcagaatg cccgcctgtt tgaaaatgcc
     1741 gtcgtagaga aaatccggcc cggacatggc gcccgtgtgg ctggcgcctg ccctggcgcc
     1801 cgaggccgaa ccacccacat actgcgcgac gacgggcttg cggggcgtga tccggcgggc
     1861 aacctccacg aaccgccggc cgtcccgaat gccttcaatg taaagaatca cggctcgcgt
     1921 ctgttcatcc tcgcccagat actccagcgc gtccacaaga tcgatgtcgg cctcgttgcc
     1981 caggctgatc gccttgctgt agcggatgcc gtttttgcgc agat
//
LOCUS       DAOSIM010000321         2014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_48353_length_2014_cov_5.797346, whole genome shotgun sequence.
ACCESSION   DAOSIM010000321 DAOSIM010000000
VERSION     DAOSIM010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48353_length_2014_cov_5.797346"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PLR47_10440"
     CDS             complement(<1..363)
                     /locus_tag="PLR47_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HOG13411.1"
                     /translation="MLFCSFCGKMQSEVRKLVAGPTAYICDECIELCRCIVEEDEKTV
                     EKGHKNVLPEPKEIKRFLDSYVIGQEKTKKILSVAVYNHYKRLFSGAGSNDGIEIQKS
                     NVLLIGPTGSGKTLLAKTL"
     gene            complement(412..1014)
                     /gene="clpP"
                     /locus_tag="PLR47_10445"
     CDS             complement(412..1014)
                     /gene="clpP"
                     /locus_tag="PLR47_10445"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOG13412.1"
                     /translation="MNLIPMVVEQDGRGERAFDIYSRLLKDRIIFLGTAIDDNVANVV
                     VAQMLYLEAEDPDKEIFFYLNSPGGHVSSGMAIYDTMQYIKPHISTVCMGQAASMAAL
                     LLAAGEKGKRFALPHSRILIHQPLGGFQGQATDIDIQAREILRLKEDLNKILAELTGQ
                     PLEKVMNDTERDYFMTSRQAKDYGLIDEIVVRKPLESGVK"
     gene            complement(1011..>2014)
                     /gene="tig"
                     /locus_tag="PLR47_10450"
     CDS             complement(1011..>2014)
                     /gene="tig"
                     /locus_tag="PLR47_10450"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOG13413.1"
                     /translation="SASFETEPEIDPQGYQGIELEKTEIRVSDADMDKRLQEIRKMFA
                     SLQDVGEDRPAAMGDFVTIDFAGTLDGEALPELKAEDYLLELGSKRFVPGFEEQVAGM
                     KKGETLDIQVKFPDDYTEQKMAGKDVTFTVAVKELKAQKLPEIDENFIKNFDKYNTLE
                     ELKEDVRKSMEEQCKRQSDVKFKELITDVALKANEFEAPPSLVERQIFYLIADTQKRM
                     KAAGMDEKSAMELSFRMHDQFKADAEKTVKSFLLFKKIAEKEAITVSDDDIDKHIGEL
                     AQIHHMNVESVRKISEDEEKKESLKADILQKKIFDFIEERANIKVVEKIGMGEEAAE"
BASE COUNT          414 a          586 c          434 g          580 t
ORIGIN      
        1 cagcgttttc gcaagcaggg ttttgccaga tccggtgggc ccgatcagca aaacattgct
       61 cttctgaatt tcaatcccgt cattcgaacc ggcgccggaa aacagccttt tgtaatgatt
      121 atatacggcg acggagagaa tttttttggt cttttcctgg ccgatcacgt aggaatccag
      181 aaaccgcttg atttccttgg gctccggcag aacattttta tggccttttt ccaccgtttt
      241 ttcgtcttct tccacaatgc agcggcacaa ttcaatgcat tcgtcgcaga tgtaggccgt
      301 cggaccggct accagtttgc gtacttcgct ttgcatcttc ccgcaaaatg agcaaaaaag
      361 cataccctgc ccccgattgt ccttgtttct tttgatcact gcctatctcc tttatttaac
      421 gccggattcc agcggtttcc ggaccacgat ttcatctatt aatccgtaat cttttgcctg
      481 ccggctggtc atgaagtaat cccgttccgt gtcgttcatc accttttcca gcggctggcc
      541 ggtcaattcc gccaggattt tgttgagatc ttccttcagc cgcaggattt ccctggcctg
      601 aatgtcgata tccgtcgcct gcccctgaaa acctcccagc ggctgatgga tgagaatcct
      661 tgaatgcggc agggcaaacc gctttccctt ttcgccggcc gccagcagaa gcgccgccat
      721 gctggccgcc tgccccatgc acacggtcga aatatggggt ttgatgtact gcatcgtgtc
      781 gtaaatcgcc attccggagc tgacatggcc gccgggcgaa ttcaaataga aaaatatttc
      841 tttatccgga tcttccgctt caagataaag catctgcgcg accactacgt tggccacgtt
      901 gtcgtcgatc gccgtcccca gaaagatgat cctgtccttc aaaagacggg aatagatatc
      961 aaacgccctt tcgccccgtc catcctgttc caccaccatg gggatcaaat tcattcagca
     1021 gcctcctcgc ccattccgat cttttccacg accttaatat tggcccgctc ctcgataaag
     1081 tcaaatatct ttttctggag gatatccgct ttcagagatt ctttcttctc ctcgtcttcg
     1141 gaaatcttcc gaacggattc gacattcatg tgatggattt gggccaactc accgatatgt
     1201 ttatcgatat cgtcatcgga taccgtgatg gcctcttttt cggctatttt tttgaaaagg
     1261 agaaaggatt tgaccgtttt ttccgcgtcg gccttaaact gatcgtgcat tcggaagctc
     1321 agctccatcg cgctcttctc gtccatgccg gccgccttca tccttttctg cgtgtcggcg
     1381 atcaggtaga aaatctgccg ctccaccagg gaaggcggcg cttcaaattc attggctttg
     1441 agcgcaacat cggtgatcaa ctccttgaat ttcacatcgc tttgccgttt gcactgctct
     1501 tccatcgact tgcggacatc ttccttcaat tcctcaagcg tgttgtactt gtcgaaattc
     1561 ttgatgaagt tttcatcgat ctctggcagt ttctgcgctt tgagctcctt tacggcaacc
     1621 gtgaacgtga cgtcttttcc cgccatcttc tgttcggtat aatcgtccgg aaatttgacc
     1681 tgaatatcca gggtttcgcc ttttttcatt cccgcaacct gctcttcaaa acccgggaca
     1741 aatcttttgg agcccagctc cagaagatag tcctccgctt ttaactcagg cagcgcttcg
     1801 ccgtccagcg tgccggcaaa atcaatcgtc acaaaatcac ccatcgccgc cggccggtct
     1861 tccccgacat cctgaaggct ggcgaacatt ttgcggattt cctgaaggcg cttatccata
     1921 tcggcatccg aaaccctgat ctcggttttt tcaagctcaa tcccctgata cccctgcgga
     1981 tcaatttccg gctccgtttc aaaggaggcg ctga
//
LOCUS       DAOSIM010000322         2003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_48705_length_2003_cov_6.469199, whole genome shotgun sequence.
ACCESSION   DAOSIM010000322 DAOSIM010000000
VERSION     DAOSIM010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48705_length_2003_cov_6.469199"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1674
                     /gene="secD"
                     /locus_tag="PLR47_10455"
     CDS             73..1674
                     /gene="secD"
                     /locus_tag="PLR47_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOG13414.1"
                     /translation="MFKSLKVRLAITAVVFLVALYFLLPTLVTQIPSPWNTYFPKDKI
                     HLGLDLQGGMHLILEVDADKALESMMQRTANDLKESLMEGKIRFRNVEKAPGATISME
                     LTDAGGKPALEKILNEQFSDLEIASSSPRDGGQIINLKIKDKRAAELKKLTIEHSVET
                     IRNRVDQFGVAEPEIVPEGENRIMVQLPGIKDPERAKNLIGKTALLEFKLVDEENSLD
                     EALRGNVPEGSIIAYGAREDKAGSQRGQAPYLLKNKTLLTGASLETAKVQISDRFGEP
                     HVSIKFNSQGAADFDRITNENVRKRLAIVLDGVVHSAPVIQERISGGQAQITGNFTMD
                     EARDLAIVLRAGALPAPVNILEERTVGPSLGSDSIRQGIMATLIGFVLVVIFMFIYYR
                     LSGLVADSVLILNVIVLLGILAGFKATLTLPGIAGIVLVIGMAVDANVLIFERVREEM
                     RLGKPPRAAIDAGYSKAFLTILDSNVTTLIAALFLFAFGTGPVKGFALTLSIGIVVSM
                     FTAIFVTRIIFDYFIWNRNVQKISI"
     gene            1702..>2003
                     /locus_tag="PLR47_10460"
     CDS             1702..>2003
                     /locus_tag="PLR47_10460"
                     /inference="COORDINATES: protein motif:HMM:NF019187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOG13415.1"
                     /translation="MEFIKPGTHFDFVGKMKIAVGISAVLIIVSILTVVLHGGLNFGI
                     DFAGGTVIQIKFSKAAPADAIRKAFAGISVEDAIIQEIGENEVIVRTNQSMTKELQ"
BASE COUNT          469 a          575 c          540 g          419 t
ORIGIN      
        1 ccattttgca gaaagcggga gctcccgcga cctctccggc gaccaagtct taaggcaaga
       61 aaggggcaac tcatgtttaa atctttaaag gtcaggttgg ccattacggc agtcgtgttt
      121 ctcgtggctc tgtatttcct tctgccgacg ctcgtgacgc agatcccctc cccctggaac
      181 acttattttc ccaaggataa aatccacctg ggactggatt tgcagggcgg catgcatctg
      241 attctggaag tggacgcgga caaggcgctg gaatccatga tgcaaaggac ggccaacgat
      301 ttgaaggaat cgttaatgga aggcaagatt cgcttccgga acgtggaaaa ggcaccaggc
      361 gccacgatct cgatggaatt gaccgacgca ggcggtaagc cggccctgga aaaaattctc
      421 aacgagcagt tttccgatct cgaaatcgcc tcctcctcgc cgcgcgacgg cgggcaaatc
      481 atcaacctga aaattaaaga taaaagagcg gccgaactca aaaaactgac catcgaacac
      541 agcgtcgaaa ccatccgcaa ccgggtggac cagttcggcg ttgccgaacc ggaaattgtt
      601 ccggaaggcg aaaaccgcat catggttcag cttcccggca tcaaggatcc ggaacgggcc
      661 aaaaatctga tcggaaaaac cgcgcttctg gagttcaaac tggttgacga ggaaaactcg
      721 ctcgacgagg cgctgcgcgg caatgttccc gaaggatcca tcatcgctta cggcgcccgg
      781 gaagataaag ccggcagcca gcggggacag gcgccctacc tgctgaaaaa caaaaccctg
      841 cttaccggcg cctcgctgga aacggccaag gtccagattt ccgaccgctt cggcgaaccg
      901 catgtttcca tcaaattcaa ctcgcagggc gcggccgatt tcgaccgcat caccaatgaa
      961 aacgtccgca aacgcctggc catcgtgctc gacggcgtgg tccattccgc gccggtcatc
     1021 caggaaagaa tttccggcgg ccaggcgcag atcaccggga acttcaccat ggatgaagcc
     1081 cgcgatctgg ccatcgttct gcgcgccggc gccctgcccg ctccggtgaa cattctggag
     1141 gaaagaaccg tcggtccttc gctgggctcg gattcgatcc gccagggcat catggccacg
     1201 ctgatcggct tcgtcctggt cgtcatcttc atgttcatct actaccgcct ttcgggactg
     1261 gtggcggatt ccgttctgat tctcaacgtg atcgtcctcc tgggcattct ggccggcttc
     1321 aaagcgacgc ttacgctgcc cggcatcgcc ggcattgttc tggtgatcgg catggcggtg
     1381 gacgccaacg tgctcatttt tgagcgcgtc cgcgaggaaa tgaggctggg aaaacctccc
     1441 cgcgcggcaa ttgacgcggg atattccaag gcctttctga ccattctgga ttccaatgtc
     1501 accacgctga tcgccgctct gttccttttc gccttcggca ccggcccggt gaagggattc
     1561 gccctgaccc tgagcatcgg cattgtcgtc agcatgttca cggccatttt tgtcacgcgc
     1621 atcatctttg actattttat ctggaaccgg aatgtccaga agatcagcat ctaggaagag
     1681 gatattacag aggagaagag aatggaattc atcaaaccgg gaacgcattt tgattttgta
     1741 ggaaaaatga aaatcgccgt gggaatatcg gcggtactga ttattgtcag cattctgacc
     1801 gtcgtcctgc acggcggttt aaatttcggc atcgacttcg cgggcggcac cgtgattcag
     1861 atcaagtttt ccaaagccgc cccggccgac gccatccgta aagcctttgc cggcatcagc
     1921 gtcgaagatg ccatcattca ggaaatcggc gaaaacgaag tcatcgtccg caccaaccag
     1981 tccatgacca aggaattgca gga
//
LOCUS       DAOSIM010000323         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_49044_length_1993_cov_8.876161, whole genome shotgun sequence.
ACCESSION   DAOSIM010000323 DAOSIM010000000
VERSION     DAOSIM010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49044_length_1993_cov_8.876161"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PLR47_10465"
     CDS             <1..382
                     /locus_tag="PLR47_10465"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOG13416.1"
                     /translation="CCARTFGFLKEVEFLQARGLGLGGSLDNAIILDDDRVINKEGLR
                     MPDEFVKHKILDAVGDLFLLGMPIIGHFVAHRSGHRLNNLLLRELMRQESCWEIISED
                     HPPQSKEFKSCNIPSFTILDAVQI"
     gene            487..1071
                     /locus_tag="PLR47_10470"
     CDS             487..1071
                     /locus_tag="PLR47_10470"
                     /inference="COORDINATES: protein motif:HMM:NF025692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HOG13417.1"
                     /translation="MKRHKNHFWTAFITGCCMMAMMAAAPAGSAQMDPRLTDILVTGG
                     AGEMLLYARLADGFKKEMEDAILAGVPAVFTIELDVYEERPYLWDRHILRREIRRTIK
                     YDNLKKNFLIATNGLEQPAILPNFESAQKSMAELNGIPVVSLSNLTRGERYYAQVRVK
                     IDRVRLPFKMEYVLFFVSLWDFETPLYKIRFAYE"
     gene            1106..>1993
                     /locus_tag="PLR47_10475"
     CDS             1106..>1993
                     /locus_tag="PLR47_10475"
                     /inference="COORDINATES: protein motif:HMM:NF039586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13418.1"
                     /translation="MRLKKDARKIYLDERELRKRRRERIIMLVVGCLAIVFTILASQL
                     SAQKELPISANILVYGLTSINIILILLLLFLIVRNVFKLFSERRKGLIGSKLRTKLVA
                     AFVGLSLVPTILLFLFAINFLSYSIEFWFNIKIGDALARSLEVAQVYYTQAEDLAKFN
                     ARQVSAEITRNRLYEVDRAGYLEHFLSQRQKNYKVARLEAYFDFNDQSLVFAEGEEAA
                     STAITLSPKMLEDIYSGKEISMVTAARGGEAVVGVAPVFSYAVATEVIGRVYVSNSVP
                     QGLVDKLRSIANASEQYGQI"
BASE COUNT          433 a          536 c          564 g          460 t
ORIGIN      
        1 ctgctgcgcg agaaccttcg gttttctgaa agaagtcgaa tttttgcagg ccagggggct
       61 ggggctgggc ggatcgcttg acaacgcgat catccttgat gacgatagag tcatcaacaa
      121 ggaaggcctc cgcatgcccg atgaattcgt caagcacaaa attctggatg ccgtaggcga
      181 tctgtttttg ctgggcatgc ccatcatcgg ccattttgtg gcgcaccggt ccggccaccg
      241 gctcaacaac ctgctcttgc gcgaactcat gcgccaggaa tcctgttggg aaatcatcag
      301 cgaagatcat ccgccgcaat cgaaggaatt caagtcctgc aacattccgt cctttaccat
      361 cctggacgcc gttcaaatct gattttgttc ccgattccct ttttttgatt ttccgaaatt
      421 acccttgatt tttaccggca ggttccctag gatacccgca ttctttcgga aagaaaacag
      481 gcgggcatga aaagacacaa aaatcatttc tggacggctt ttatcaccgg ttgctgcatg
      541 atggccatga tggctgccgc tccggcggga agcgcacaga tggatccccg gttgacggat
      601 attttagtga ccggcggcgc gggggaaatg ctcctgtacg cgagactggc ggacggcttc
      661 aaaaaggaaa tggaggacgc catcctggcg ggggtccccg ccgtttttac gattgagctg
      721 gatgtctatg aggaaaggcc ctatctgtgg gaccggcata ttctccgcag ggaaatccgc
      781 cgcaccatca aatacgacaa tttgaaaaag aattttttga tcgccacaaa cggcctggag
      841 cagccggcga ttctgccgaa ttttgaaagc gcgcaaaaat cgatggcgga gttgaacgga
      901 attcccgtgg tttccctttc caatctgacc cgcggcgaac gctactacgc gcaggtgcgg
      961 gtcaaaatcg accgtgtgcg tctgccgttc aagatggaat acgtgctgtt ctttgtgtcc
     1021 ctgtgggact ttgaaacccc cctgtacaag atccggttcg cctacgagtg atcgcggggg
     1081 cggggaaggg tgatgacgca agcgcatgag gttgaaaaaa gacgccagga aaatctatct
     1141 tgatgaacgg gagttgagaa agcgccggcg cgagcgcatc atcatgctgg tggtcggctg
     1201 cctggcgatt gtttttacga ttctggccag ccagttgtcc gcccagaaag agctgccgat
     1261 ctcggcgaac attctggtgt acggcctgac cagcatcaac atcatcctga tcctgcttct
     1321 gctttttctg attgtccgca atgtcttcaa gctgttttcc gagcggcgca agggattgat
     1381 cggctcgaag ctccgcacca agctggtggc cgcgttcgtg ggcctgtccc tggttccgac
     1441 cattctgctt tttttgtttg ccatcaactt tctttcctat tcgatcgaat tctggttcaa
     1501 catcaaaatc ggcgacgccc tggcgcggtc gctggaagtc gcgcaggtgt actacacgca
     1561 ggccgaggac ctggccaagt tcaacgcccg tcaggtcagc gcggaaatca ccagaaaccg
     1621 cctctacgag gtggaccggg ccggttacct ggagcatttt ctttcccagc ggcaaaagaa
     1681 ctacaaggtg gctcggctgg aagcgtattt tgatttcaat gaccagagcc ttgtctttgc
     1741 cgaaggcgaa gaggcggcgt ccaccgccat caccctgtcg ccgaaaatgc tggaagacat
     1801 ctattccgga aaggaaatat ccatggtgac cgccgcccgc ggcggggagg ccgttgtcgg
     1861 cgtggcgccg gttttctcct atgccgttgc gacggaagtc atcgggcggg tttacgtcag
     1921 caacagcgtt ccccagggac tggtggacaa gctcaggagc atcgccaacg cgtcggagca
     1981 gtacggacag atc
//
LOCUS       DAOSIM010000324         1992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_49084_length_1992_cov_9.177594, whole genome shotgun sequence.
ACCESSION   DAOSIM010000324 DAOSIM010000000
VERSION     DAOSIM010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49084_length_1992_cov_9.177594"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PLR47_10480"
     CDS             <1..241
                     /locus_tag="PLR47_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG13419.1"
                     /translation="FELLVSDVDMPRMNGIDLVRTVKADPQHHDMPVMIVSYKDREED
                     RLRGLEAGANYYLTKGSFHDESLLTAVRDLIGGPE"
     gene            238..1275
                     /gene="cheB"
                     /locus_tag="PLR47_10485"
     CDS             238..1275
                     /gene="cheB"
                     /locus_tag="PLR47_10485"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF009206.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HOG13420.1"
                     /translation="MTLKIAIAHHDENALKILRRIIAPNAGCAVIWTTGRGSDVIRQC
                     RHGLPDILLIDLELADVNGAKLICDVMKETPCAILVLSNARERQAGKVFEAMGCGALD
                     VIATPVLHSNGFLEGADALLKKIDTMARLLGTASRMEKPAATVHETKKEPRPPLVAIG
                     SSTGGPKALATILAGLPGNFPAAIVICQHVDSQFASGLASWLNSQTPLDVAIAREGMR
                     PRPGAALVAQTNDHLVLGPDGSLHYTEHPRNYPYRPSVNALFESLRQYWPQNDVAILL
                     TGMGRDGGEGLSALFKTGWHTIVQDEKTSVVYGMPAAAVELGGASEILPLDSIAESIA
                     KKITNRKGKPS"
     gene            1272..>1992
                     /locus_tag="PLR47_10490"
     CDS             1272..>1992
                     /locus_tag="PLR47_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG13421.1"
                     /translation="MTEKENQNPAPSGSRDPATILTDHKIKVLLIDDQAIVGAAVKRM
                     LEPETDIVFQFCQDPTQAIPTAASFQPTVILQDLIMPDIDGLTLVKFFRVHPQLKDVP
                     LIVLSSKEEAVTKADAFALGANDYLVKLPDRIELVARIRYHSRGYINLLQRNEAYAAL
                     LASRQALAAELAQAADYVVSLMPPPLSDPTITTAWRFFPSAQLGGDSFGYHWLDENTF
                     AVYLLDVCGHGVGSALLSVSAF"
BASE COUNT          414 a          651 c          557 g          370 t
ORIGIN      
        1 ctttgaactg ctcgtttccg acgtggacat gccgcgcatg aacggcatcg atctggtccg
       61 gacggtcaaa gccgatccgc agcaccacga catgccggtc atgattgtct cctataaaga
      121 ccgtgaagaa gaccggctgc ggggcctgga agccggcgcc aattactatc tgaccaaggg
      181 cagctttcat gacgaaagtc tgcttaccgc cgttcgcgac ctgatcggag gacccgaatg
      241 acgctcaaaa tcgccattgc ccatcacgat gaaaacgccc tgaaaatcct gcgccgaatc
      301 attgccccga atgccggctg cgcggtcatc tggaccacgg gccgcggaag cgatgtcatc
      361 cggcaatgcc gtcatgggct gccggatatt ctgctcatcg atctggagct tgccgacgta
      421 aacggcgcga agctgatctg tgacgtgatg aaagaaaccc cctgcgcgat cctggtcctg
      481 tcgaatgcca gggaacggca ggcggggaaa gtattcgagg ccatggggtg cggtgcgctg
      541 gatgtcattg ccacgcctgt gcttcattcc aacgggtttc tggaaggggc agacgccctt
      601 ctcaaaaaaa tcgacaccat ggccaggctt ctgggaaccg cgtcccgaat ggaaaaaccg
      661 gctgcgaccg tccatgaaac gaaaaaagag ccgcggccgc ccctcgtcgc catcggttct
      721 tcgacgggag gccccaaggc cctggccacg attctcgccg gcctccccgg gaattttccc
      781 gccgcgatcg tcatctgcca gcatgttgat tcgcagtttg cctcgggcct cgcctcctgg
      841 ctgaattccc agacgccgct tgacgtagcc attgcccgcg aaggcatgcg gccccggccc
      901 ggcgccgccc tggtggccca aaccaacgat caccttgtcc ttggaccgga cggatcgctt
      961 cactacacgg agcacccccg aaattatccc tatcgtccgt ccgtcaacgc gctttttgaa
     1021 agtcttcggc aatactggcc gcaaaatgac gtggcgatcc tcctcaccgg catggggcgg
     1081 gacgggggag aaggcctgtc ggctctcttt aaaaccggct ggcacacgat cgtccaggat
     1141 gaaaagacct ccgtcgtcta tggcatgccg gcggcggccg tggaactagg cggcgcttca
     1201 gaaatcctgc ccctggacag catcgccgaa tccattgcaa aaaaaatcac caaccgaaaa
     1261 gggaaaccat catgaccgaa aaagaaaatc aaaaccccgc cccgtccgga agccgggacc
     1321 cggcaaccat actgacggac cacaaaatca aggtcctgct catcgacgat caggcgattg
     1381 tcggcgcggc ggtgaaaaga atgctcgagc cggaaacgga catcgttttt cagttctgcc
     1441 aggaccccac gcaggccatc cccaccgccg cctcgtttca gccgacggtg atccttcagg
     1501 acctgattat gccggacatc gacggcctga cgctggtgaa atttttccgc gtccatcccc
     1561 agctcaagga cgtgcccctg atcgttttat cttccaagga agaggcggtc acgaaagcgg
     1621 acgccttcgc gctcggcgcc aatgactacc tggtgaagct tcccgaccgc atcgaactcg
     1681 tcgcccgcat ccggtaccat tcccggggct atatcaacct gctgcagcga aacgaagcct
     1741 atgccgcgct gctcgccagc cggcaggcgc tggccgcgga gctggcccag gcggccgact
     1801 acgtggtgtc gctcatgccg cccccgctgt ctgatccgac gatcacgacg gcatggcgct
     1861 ttttccccag cgcccaactg ggcggcgaca gcttcggcta ccactggctc gatgaaaaca
     1921 cctttgccgt ttacctgctg gacgtctgcg ggcacggggt cggctcggcg ctgctttccg
     1981 tttccgcctt ca
//
LOCUS       DAOSIM010000325         1957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_50315_length_1957_cov_7.430599, whole genome shotgun sequence.
ACCESSION   DAOSIM010000325 DAOSIM010000000
VERSION     DAOSIM010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50315_length_1957_cov_7.430599"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            397..1440
                     /locus_tag="PLR47_10495"
     CDS             397..1440
                     /locus_tag="PLR47_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13422.1"
                     /translation="MKPIVKMTLAMAAVLFFTSCAAGHNACLKSRMDRHEVRSPAFWQ
                     DYRRGNLEDRLHAAPEPLLDYLHLDNRLNGYDTRPQATVADDSFRQDVRQAMAELPGP
                     VREAVKEKLIGIFFVRDLGTTGYTEAVADPQGRPAAGFVVLDVGSLRQTANAWATSRE
                     NTVFQNDGEGRLQVTLEEAPNDNSKNAIQFILLHEFGHLVSIGERFHPSWLDEAKPAG
                     AIEDYLFYPLSWRKEKDGSNVSLFEDVFPERRDVRFYGEARLKSPQMADVYRRLAMTN
                     FVSLYGATGPFEDFAESFALYVHSVWMKKPYRVQITQSGRPVFLYESCWDQPRCAAKR
                     AVLEEWFSRFRKP"
     gene            complement(1461..>1957)
                     /locus_tag="PLR47_10500"
     CDS             complement(1461..>1957)
                     /locus_tag="PLR47_10500"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOG13423.1"
                     /translation="LKKQGFKNEEITWQPSKTLDKLAREYGQNEKNDRFRYVVTSSLR
                     VTTKNIDMVIRALGATEELLKAGILLDGQQGEGMANPRYVVTTFNALRPQLLAEATKN
                     ARFTAEQFASDSGARIGKIRSANQGMIQIFGSEGNDESAPYSPTSTPVKKIRVVSTFE
                     FELK"
BASE COUNT          389 a          524 c          566 g          478 t
ORIGIN      
        1 gtagaactcc cgcgatactt tttccttcaa caattttgag gtcaagcgcc gtgtacgagt
       61 gccgatgtcc ccatgatggc aagaagcttg ccgaactggc ccggccacct ttggctttgg
      121 gtcattataa ctacccctgc gagtgcgggc ggtgggtgca gggaaagatc atcctggagg
      181 aagggacgga aagcatcctg gcggttgatt cctgttcctg cggacgtaca accagacggg
      241 tagttggaca tctggtgacg atccagtgtc ctaaatgccg gttttttgca atctgtggtt
      301 gatacatcaa atttatattc gtataatgcg cctgcgatgg aggtgtttta tacggaataa
      361 attaaccatc tcaaatgcgt agtgtcgggg aaatcgatga aacccattgt aaaaatgacg
      421 ctggcgatgg cggcggtgct tttcttcacg tcctgcgccg ccggccacaa cgcttgcctg
      481 aaaagcagga tggatcgtca tgaagtccgc tcgccggcgt tttggcagga ctaccggcgg
      541 ggcaacctcg aagacaggct tcatgcggct cccgaaccgc tcctggatta tctccatctg
      601 gataaccgtt tgaacgggta tgacaccaga cctcaagcga cggtcgcgga tgattcgttc
      661 cggcaagatg tgcggcaggc catggccgaa ctgcccgggc cggttcggga ggcggtgaaa
      721 gaaaaactga tcggaatctt tttcgttcgc gaccttggaa cgacgggata cacggaggcg
      781 gttgccgatc ctcagggaag gcccgccgcc ggtttcgttg tcctggatgt cggatcgctt
      841 cgacagaccg ccaatgcttg ggccacttcc agagagaaca ccgttttcca aaatgacggc
      901 gagggccggc ttcaggtcac gctggaggaa gcgccaaatg acaattccaa aaacgccatc
      961 cagttcattc tgctgcacga gttcgggcac ctggtttcca tcggcgagcg ctttcatccg
     1021 tcctggctgg acgaagcgaa accggccggg gcgatcgaag attacctttt ctatcctctt
     1081 tcctggcgca aggaaaaaga cggctcgaat gtttctctgt ttgaggatgt ttttccggag
     1141 cgtcgggatg tgcgctttta cggagaggcc cgcctgaaat ccccgcagat ggcggatgtt
     1201 taccgccgcc tggccatgac gaatttcgtt tcgctctacg gggcgaccgg cccttttgaa
     1261 gactttgccg agtcgttcgc gctgtatgtc cacagcgtct ggatgaaaaa accctatcgc
     1321 gtgcaaatca cgcaaagcgg ccgtccggtt tttctctacg agtcctgctg ggaccagccg
     1381 cggtgcgccg ccaaaagggc cgttctggaa gagtggtttt cgcgttttcg caagccctga
     1441 cccgcgccgt ttgttcttga ctatttcaac tcaaactcga aggtgctgac cacacgtatt
     1501 ttttttacgg gcgtgctggt cggactgtag ggcgcggatt catcgttccc ctcgctgccg
     1561 aaaatctgga tcatgccctg attcgctgag cgaatttttc cgatccgggc gccggaatcc
     1621 gaggcaaact gctcggcggt gaaccgggcg tttttggtgg cttcggcaag caattgcggc
     1681 cgaagagcgt tgaaggttgt aacgacgtat cgcggattgg ccatgccttc tccctgctgt
     1741 ccgtccagaa ggattcccgc cttaagcagc tcttctgtgg cccccagcgc ccgaatgacc
     1801 atgtcgatgt ttttggtcgt cacccgcaga gaactggtga ccacgtagcg gaaccggtcg
     1861 ttcttttcgt tctggccgta ttcgcgggcc agcttgtcaa gcgtttttga gggctgccag
     1921 gtgatctctt catttttgaa gccctgtttt ttgagga
//
LOCUS       DAOSIM010000326         1904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_52292_length_1904_cov_7.790157, whole genome shotgun sequence.
ACCESSION   DAOSIM010000326 DAOSIM010000000
VERSION     DAOSIM010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52292_length_1904_cov_7.790157"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /gene="mraY"
                     /locus_tag="PLR47_10505"
     CDS             <1..435
                     /gene="mraY"
                     /locus_tag="PLR47_10505"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOG13424.1"
                     /translation="FAGNIKVAHYLQIPYVPGAGELSVFCGAFMGAALGFLWFNAHPA
                     EIFMGDVGSLSMGGALGAVALITKQEILLAIVGGVFVMETFSVIFQVGYFKLTGGKRI
                     FRMAPIHHHFELKGWAEPKVIVRFWIISILLALLAISTLKLR"
     gene            444..1787
                     /gene="murD"
                     /locus_tag="PLR47_10510"
     CDS             444..1787
                     /gene="murD"
                     /locus_tag="PLR47_10510"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOG13425.1"
                     /translation="MQLAGKKIAVIGMGKTGIAAAVFLDDNGAAVVAVDEKPRSRWGA
                     EFEQIAHRPRLVLGRYDETALDGADLVVPSPGIPPRHPILTAARQKKIPILSEIELAY
                     RFIRVPIIAVTGTNGKTTTTTLLGEILARAGKKVHVGGNIGNPLIEYAGTAQTDDFIV
                     AEISSFQLQWAETFSPHAAILLNITCDHVNYHGSFEEYRRIKGRIFEHQRENDLAVLN
                     AEDPHQEGLGPSLRAAVVRFSSVRPQSPGLCLENGCIVYRHPGGSEERYPSEMIRLPG
                     LHNVENVMAAVAAARFCGCSKESITEAVSQFRGLPHRIEFAGEKRSVVFYDDSKGTNV
                     DAVVRALQTFSSPVILLLGGRDKDGNFEALKPLLRAKTKQVVLFGEARDRIEPQLGGA
                     IPIAKETTLKEAVLRAYRDAVPGDIVLLSPGCASFDAFANYKERGNHFKEVVGNL"
BASE COUNT          383 a          515 c          599 g          407 t
ORIGIN      
        1 ttcgccggca acatcaaggt tgcgcattat ctgcagattc cctatgtgcc cggcgcgggc
       61 gagttgtcgg tattctgcgg agcgttcatg ggcgcggcgc tgggcttttt gtggtttaac
      121 gcccacccgg cggaaatttt tatgggggat gtcggatccc tgtccatggg gggcgctctg
      181 ggggccgtgg cgctcatcac caaacaggaa attctgctgg cgattgtcgg cggagttttt
      241 gtaatggaga cgttttccgt tattttccag gtagggtatt tcaagctgac gggcggcaaa
      301 cggatttttc gaatggcgcc gatccatcat cattttgagc tcaagggctg ggcggaaccg
      361 aaggttattg tccggttctg gatcatttcg atcctgctgg cgcttctggc gatcagcaca
      421 ttgaagttgc ggtagggaaa agcatgcaac tggcgggaaa gaaaatagcc gtgatcggca
      481 tgggaaaaac gggtatcgcc gccgccgtgt ttctggacga caacggggcg gcggtcgtgg
      541 cggtcgacga gaagccccgc agccggtggg gtgcggagtt cgagcaaatc gcccatcggc
      601 cgcggcttgt cctcggacga tacgacgaga cggctctgga cggcgcggac ctggtggtgc
      661 cgtctccggg aatccccccc cgccatccga ttctgacggc ggccaggcaa aaaaagattc
      721 cgatcctgag cgagatcgaa ctggcctacc gcttcatccg cgtgccgatc atcgccgtaa
      781 cgggcaccaa cgggaaaacc accaccacca cgctgctggg cgagattctg gcgcgcgccg
      841 ggaaaaaagt gcatgtcggc ggcaacatcg gaaatcccct gatcgaatac gccggcaccg
      901 cgcagacgga tgatttcatt gtcgctgaaa tcagcagttt ccagctccag tgggccgaaa
      961 cgtttagccc gcatgccgcg attctgctca acattacctg cgatcacgtc aactatcacg
     1021 gatcgtttga agaataccgc cgcatcaagg ggaggatatt tgagcatcag cgcgaaaacg
     1081 acctggcggt tctgaacgcc gaagatccgc atcaggaagg cctgggcccg tcgctcaggg
     1141 ccgccgtggt ccgcttcagc tccgtccggc cgcaaagtcc tggattgtgc cttgagaacg
     1201 gctgcatcgt ttaccggcat cccggcgggt ctgaggagcg ctatccttcg gagatgatcc
     1261 gccttcccgg tctgcacaat gtggagaatg tcatggctgc cgtggcggcg gcgcgcttct
     1321 gcggctgttc aaaagagagc atcacggagg cggtgtctca gtttcggggc ctgccgcacc
     1381 ggattgaatt tgccggagaa aaacggtcgg ttgtctttta tgacgactcc aaggggacca
     1441 atgtcgatgc cgtcgtgcgc gcgctgcaaa ctttttcatc gccggtgatc ctgcttttgg
     1501 gcggacggga caaggacgga aatttcgagg cgctcaagcc gcttttgcgc gccaaaacca
     1561 aacaggtcgt tctgttcggc gaggcgcgcg accggattga gcctcaattg ggcggggcga
     1621 ttccgatcgc gaaggaaacg acgttgaaag aggccgtcct tcgcgcttac cgggacgccg
     1681 ttcccggaga cattgtcctc ctgtcgccgg gatgcgcgag ttttgacgcg tttgccaatt
     1741 acaaggagcg gggaaaccat ttcaaggaag tggtagggaa tctgtaaatg gacgctgcaa
     1801 aaaaagacgc caatacgccg gatgtcattc ttttgatggt caccctgatt ctggtgaccg
     1861 tgggcacggc gatgatttat tcgtcgagct ccattctggc gatg
//
LOCUS       DAOSIM010000327         1889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_52870_length_1889_cov_6.169575, whole genome shotgun sequence.
ACCESSION   DAOSIM010000327 DAOSIM010000000
VERSION     DAOSIM010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52870_length_1889_cov_6.169575"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLR47_10515"
     CDS             <1..449
                     /locus_tag="PLR47_10515"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOG13426.1"
                     /translation="EIEKRAQVRHVCRMFVVGSHVTVPYFTVILRRGYGLGVMAMARG
                     GFHQSFFSVSWPTGEFGGMGLEGAVKAGFKKELAAIEDLQKREEFYESLVAQLYEIGK
                     AINMAAHLEIDAVIDPADTRRWIMRGLKSAPARQPADAGHSFVDSW"
     gene            complement(425..1321)
                     /locus_tag="PLR47_10520"
     CDS             complement(425..1321)
                     /locus_tag="PLR47_10520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HOG13427.1"
                     /translation="MNRRGALLVFIVFFLAWPALAQAAGDDPRAAVEELLVAEGLDPA
                     KVRSLLQDSRVSVRPEIVLKNLFFSKPKGSGKDPDVMEIDPRQIERGREFMKENDAVL
                     SDVERRFGTSPRIITAILIIESRLGTYPMPYNVVNAYANLAFMADPAYWEEIQSRYGD
                     AYPEIRSDAAAARAKQKAKWAVRELYHLSQIADHLGIDPLSLSGSFAGAMGPAQFIPS
                     SFWVFGMDGNGDGLADPSNMTDANFSMGHYLRKFGWAENASVEQKRRAIWHYNRSRVY
                     VNTVMMLYEELGRKNHQESTNE"
     gene            1531..1842
                     /gene="rplU"
                     /locus_tag="PLR47_10525"
     CDS             1531..1842
                     /gene="rplU"
                     /locus_tag="PLR47_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOG13428.1"
                     /translation="MYAIIKTGAKQHKVSEGDVLSVEKLPGDKGAEVVFQEVLMVSDE
                     AGVKIGRPFVEGATVIGEVVSQTKGPKLLIGKYKKRKGFRKKTGHRQELTSMKIKKIS
                     I"
BASE COUNT          444 a          513 c          520 g          412 t
ORIGIN      
        1 ccgaaattga aaaacgggcg caggtccgcc atgtctgccg gatgtttgtc gtcggctcgc
       61 atgtcaccgt gccttacttc acggtcatcc tgcgccgggg ctacggactc ggcgtcatgg
      121 cgatggccag gggcggattt catcaatcat tcttcagcgt gtcctggccg accggggagt
      181 tcggcggaat gggactggaa ggcgccgtca aagcaggctt caaaaaagaa ctggcggcca
      241 ttgaggacct gcaaaaaaga gaagagttct atgaatcgct ggtggcgcag ctctacgaaa
      301 tcggcaaagc catcaacatg gccgcgcacc tggaaatcga cgccgtcatc gatccggccg
      361 acacccgccg gtggatcatg cggggtctga aatccgcgcc cgcccgacag ccggcggatg
      421 caggtcattc atttgtcgat tcctggtgat ttttccgtcc cagttcttcg tagagcatca
      481 tcacggtatt gacgtacacg cggctgcggt tgtagtgcca gattgccctt cgtttttgtt
      541 caacggaggc attctccgcc cagccgaatt tcctgagata atgccccatg ctgaaattgg
      601 catccgtcat attggaggga tcggccaatc cgtccccgtt gccgtccatt ccaaaaaccc
      661 agaaggagga tggaataaac tgcgccgggc ccatcgcgcc ggcaaaagat cccgataggc
      721 tcaatggatc gattccaaga tgatcggcga tctggcttaa gtgatacagc tcccgcaccg
      781 cccactttgc cttttgtttc gcccgggcgg cggccgcgtc gcttcgaatc tcgggataag
      841 cgtccccgta gcggctctgg atttcctccc agtaggccgg atccgccata aaggccagat
      901 tggcgtaggc gttgaccacg ttgtacggca tgggataggt tcccagccgg gattcaatga
      961 tcagaatcgc cgtgatgatc ctcggcgacg tcccgaagcg ccgctcgacg tcggacaaaa
     1021 cagcgtcgtt ttccttcatg aattctcttc cccgttcgat ctgacgaggg tcgatttcca
     1081 taacatcggg atcctttccg gaccctttgg gctttgaaaa gaaaaggttt ttcagcacaa
     1141 tctccgggcg aacggacaca cggctgtcct gcaggagact gcgaaccttc gcgggatcaa
     1201 gcccttcggc gaccagcagt tcctcgactg ccgcccgcgg gtcatcgcct gccgcttgcg
     1261 caagcgcagg ccaggccaga aaaaaaacaa tgaaaacgag aagagcgccg cgtctgttca
     1321 taaatacacc tcgatcgttt ctgcctgccg cctgccgttg atcctacctt ttggccgccg
     1381 tggaatgcaa gcgattttct tcttaatatc ggcattttag ccatgcccgc gggcggccgg
     1441 ataaaatatc gcgagggctg gcgaatttta ttgacaaaaa ggttcatttt gtctataagc
     1501 ttcgttctta cttcagcaaa ggataaggat atgtacgcga ttataaaaac aggagccaaa
     1561 cagcacaaag tcagcgaggg cgatgtatta tccgttgaaa aactgcccgg tgacaagggc
     1621 gccgaagttg tttttcagga agttttgatg gtgtcggacg aggccggtgt aaaaatcggc
     1681 aggcccttcg ttgaaggcgc caccgttatc ggtgaagtgg tctcccagac caaaggaccg
     1741 aagctgctca tcggcaaata taaaaaacgc aagggttttc gaaagaagac cggtcatcgt
     1801 caggaattga ccagcatgaa aatcaagaag atttcgatat aggcggagga catcatggca
     1861 cataaaaaag gtcagggaag ctcccgcaa
//
LOCUS       DAOSIM010000328         1884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_53061_length_1884_cov_8.459814, whole genome shotgun sequence.
ACCESSION   DAOSIM010000328 DAOSIM010000000
VERSION     DAOSIM010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53061_length_1884_cov_8.459814"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..908
                     /locus_tag="PLR47_10530"
     CDS             234..908
                     /locus_tag="PLR47_10530"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOG13429.1"
                     /translation="MENRQKEGLEVSRREMLILSSGFVTALMLGNMPGAGLSHAAEIK
                     FPEGKCTGKMEKKVLVAYASKYGSTGGVADAVAKELCAKGVSADVTLIRNARNIGSYQ
                     GVVVGSAIYMGKWMPEAVDFVKKNKEALRRVPVAYFLVCMTLARPTDQKRAEVLAYMD
                     PVLQEVPEIKPVGLGTFAGAMHYGNLSWIYKKILQSKGTPEGDYRDWNALRSWAQEPV
                     YAKFFR"
     gene            complement(991..>1884)
                     /locus_tag="PLR47_10535"
     CDS             complement(991..>1884)
                     /locus_tag="PLR47_10535"
                     /inference="COORDINATES: protein motif:HMM:NF024040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13430.1"
                     /translation="DFTINALAYNIADFSVIDYTTGLADLRDGLIRPIGDPYVRFTED
                     PVRMLRAVRFAASHDFVIEPAAWETLCTLSSTISRASPARLYEEMLKLFLLGAAGRAF
                     GLLNISGLLAALFPMLSNWLGGNTQRLNIMNANLETLDCRCRMGMPPSPALFFAAFFG
                     PLLAETTLAGRRDGIPGRLTRDAACTDLMKECSETVSIPGKVANQLRNILAMQHSLHS
                     VPPRRPSVIVKRPEFADALAYLHLEGTLGGENQSALEWWDAFLSGKSSSIIAETPADE
                     ASAKRRRRRKRRHRAKGTPVK"
BASE COUNT          439 a          502 c          574 g          369 t
ORIGIN      
        1 aggcttccct tcatacgctc cggcagcttc aggttcccga ggccgccccc agcgcgatgc
       61 cgccgttgtt gaatccacga aacaggttca ggttcagaac tctcagaaaa aatataatgc
      121 cggatacccc cggcatctat ggttgatcct ccggccaggg gcttccattc tgaacgcatc
      181 gggagtatat ggcagtgaaa caatccggta aaaaagcaga aagggaggct cttgtggaaa
      241 acaggcagaa ggaaggattg gaagtcagtc gacgggaaat gctgatcttg tccagcggct
      301 ttgtcacggc cctcatgctt gggaatatgc cgggggccgg gctcagccac gcagcggaaa
      361 taaaatttcc ggaagggaaa tgcacaggca aaatggaaaa gaaagtattg gtcgcgtacg
      421 ccagcaagta cggttcaacc gggggagtcg ccgatgccgt cgcgaaagag ctttgcgcca
      481 aaggcgtaag cgccgacgtg actttgatca ggaatgcccg caacatcggg tcctatcagg
      541 gcgtggtcgt cgggagcgcc atctacatgg gcaagtggat gccggaagca gtggatttcg
      601 tcaaaaagaa caaagaagct ttgcggcggg tgccggtcgc ctatttcctc gtgtgcatga
      661 cgcttgccag accgactgat cagaaacggg ccgaagtgct ggcctatatg gaccccgtcc
      721 tgcaggaagt gccggaaata aaacctgtcg gccttggaac atttgccggc gcgatgcatt
      781 acggcaacct gtcctggatc tacaaaaaga ttttgcagtc caagggaacc cccgagggag
      841 attaccgcga ctggaatgcc ctccgttcct gggcgcaaga accggtgtat gccaaatttt
      901 tccggtaaaa aatcccgaag ccggtaaatg ccggatcata accggttcag ccaaggccac
      961 aaaggctctg attccgccgt gagaaacctg ctactttacg ggtgtccctt ttgcccgatg
     1021 tctgcgtttc cgccttctgc gccgtttcgc cgacgcttca tcggccggcg tttccgcaat
     1081 gatggaagaa gacttccctg aaagaaacgc atcccaccac tccagtgcgg attgattttc
     1141 ccctcccaac gttccttcga ggtgcaggta ggccagggca tccgcaaatt cgggtctctt
     1201 gacgatgacg gaaggacggc gcggcggcac gctgtgaagc gagtgctgca tggcaaggat
     1261 gttgcgcagc tggtttgcga cctttccggg aatgctcacg gtctcggagc actctttcat
     1321 cagatccgta cacgcggcat cccgtgtcaa tcggccgggg atgccgtccc ggcggccggc
     1381 aagcgttgtt tccgcgagga ggggaccgaa gaatgcggca aagaagagcg ccggggacgg
     1441 cggcattccc atccggcatc ggcagtccag ggtttcaaga tttgcgttca tgatgttcag
     1501 acgctgggtg tttccgccaa gccagttgct gagcatggga aagagggcgg caaggagccc
     1561 gcttatgttc agcagaccga aagcccgccc tgcggcgccg agcaggaaga gtttcaacat
     1621 ctcttcatag agccttgccg gtgacgcgcg ggaaatggtg gaggaaaggg tacagagggt
     1681 ctcccacgcg gcaggttcga tgacaaaatc atgggatgcg gcaaaacgaa cggcgcggag
     1741 catgcgcacc gggtcttccg taaatcgtac gtacggatcg ccgatggggc gaatgagccc
     1801 gtcgcgcaga tcggcgagtc ccgtcgtgta gtcgatgacc gaaaaatctg cgatattata
     1861 ggcaagggca ttgatcgtaa agtc
//
LOCUS       DAOSIM010000329         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_53730_length_1868_cov_7.177606, whole genome shotgun sequence.
ACCESSION   DAOSIM010000329 DAOSIM010000000
VERSION     DAOSIM010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53730_length_1868_cov_7.177606"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PLR47_10540"
     CDS             <1..352
                     /locus_tag="PLR47_10540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416543.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOG13431.1"
                     /translation="QYRELAAFAQFGSDLDKSTQAQLDRGVRLVELLKQPQFQPMSLG
                     EEVVVLFAGSRGFIDKVDVEKVLVYEQQLLSYVKSKHQDILKEIDEKQIISADLEKKM
                     KDMLTGFESVFVAE"
     gene            430..1308
                     /gene="atpG"
                     /locus_tag="PLR47_10545"
     CDS             430..1308
                     /gene="atpG"
                     /locus_tag="PLR47_10545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOG13432.1"
                     /translation="MPSLKDIKRKVTAVQKTKQITRAMNMVAASKFKSAQLKMENFRP
                     YAGKFMDVLNSLALRVDTSTHPLLAVRDPRRIRVICMTSDRGLCGGFNTNLIKATERF
                     MRDKTRDGKEVSMISVGRKGRDFFRKKANVLAQKVDCLSKFDMTLAVSVADEVIDPFI
                     KAEYDELYLIYNQFVNVSVQRPTVVRLFPLPSIGSPDADMETDTRLDYVYEPSDEVLL
                     HKLLPMYVHVLVYRALLETSAGENGARMAAMDNATSNCEELIHTLTLKMNKARQAAIT
                     AELMDIVGGTEALAKG"
     gene            1370..>1868
                     /locus_tag="PLR47_10550"
     CDS             1370..>1868
                     /locus_tag="PLR47_10550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416545.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOG13433.1"
                     /translation="MNIGKIVQVIGPVVDVAFEEGKLPSILNALLVTNPAINDQEDNL
                     VIEVAQHLGDNVVRCIAMDITDGLVRGMNAKDTGAPITVPVGKEGLGRILNVVGRPVD
                     GLGPVNAKNFAPIHRESPAFLEQDTSVHVLETGVKVIDLLVPFPRGGKMGMFGGAGVG
                     KTVVMM"
BASE COUNT          455 a          446 c          539 g          428 t
ORIGIN      
        1 ccagtaccgc gaactggcgg cctttgccca gttcggctcc gatctggaca aatcgacgca
       61 ggcccagctc gaccgcggcg ttcgtctggt cgaattgctg aagcaacccc agttccagcc
      121 gatgtcttta ggcgaggaag tggtggttct gtttgccgga agccgcggct tcatcgacaa
      181 ggtggacgtg gaaaaggttc tggtctatga gcagcagctg ctgagctacg taaagagcaa
      241 acatcaggat atcctgaaag aaatcgatga aaagcagatt atttccgcgg acctggaaaa
      301 gaaaatgaaa gatatgctga cgggtttcga atctgttttc gtagcggaat aaattgtaat
      361 ccatgcgcgg tcctcaaaaa gaggatctcc atggagcgga aaaccaatta atgttgagag
      421 gagtttccag tgccctcgct aaaagatatt aaaagaaaag taacggccgt tcagaaaacg
      481 aagcagatta cgcgcgccat gaacatggtg gctgcatcca agtttaaatc cgctcaactg
      541 aaaatggaaa atttccggcc ctatgccgga aaattcatgg atgttctcaa cagtctggcc
      601 cttcgggtgg acacctccac gcacccgctt ctggccgtgc gcgatcccag aagaatccgg
      661 gtgatctgca tgacgtccga ccggggactc tgcggaggct tcaatacgaa tctgatcaag
      721 gcaacggaac ggtttatgcg ggacaagacc cgtgacggca aggaagtttc catgatcagc
      781 gtcggccgga agggcaggga ttttttccgc aaaaaggcaa acgtcctggc gcaaaaagtg
      841 gactgtttaa gcaagtttga catgaccctc gcggtgtcgg tcgcggatga agtgattgat
      901 ccctttatca aggcggagta cgatgaattg tatttgattt ataatcagtt cgtgaatgtt
      961 tccgttcaga ggccgaccgt tgtccgcctg ttcccgctgc cctccatcgg cagcccggac
     1021 gccgatatgg aaacggatac gcggctggat tatgtttacg aaccgtcgga cgaggtgctc
     1081 ttgcacaaat tgctgccgat gtatgttcat gttctggtgt atcgggctct gctggaaacg
     1141 tccgccggcg aaaacggcgc gcggatggcg gcgatggaca acgccaccag caactgcgaa
     1201 gagctgatcc acacgctgac gttgaaaatg aacaaagcgc ggcaggcggc gattacggcg
     1261 gaattgatgg atatcgtagg cggtacggaa gctttggcga aaggttgatt ttgcggcggc
     1321 attttgcgcg taaaaaatat aaattacgac tattggcaag gagatagtta tgaatatcgg
     1381 aaagattgtt caggttattg gtcccgttgt tgacgtggct ttcgaagaag ggaaactgcc
     1441 cagcattttg aatgcgcttt tggttaccaa ccccgcgatc aacgatcagg aagataactt
     1501 ggttattgaa gtcgcgcagc atctgggtga caacgttgtc cgttgcatcg ccatggatat
     1561 caccgatggt cttgtgcgcg ggatgaacgc caaggatacc ggcgcaccga ttaccgttcc
     1621 ggtcggcaaa gaaggtctcg gccgaattct gaacgtcgtc ggtcgtcctg tggatggctt
     1681 gggaccggtc aacgccaaga atttcgctcc gattcatcgt gagtctcccg cctttctgga
     1741 gcaggatacc tccgttcacg ttctggaaac aggtgttaag gttatcgatc tgctcgttcc
     1801 gtttccccgc ggcggcaaga tgggcatgtt cggcggcgcc ggcgtcggca agaccgtcgt
     1861 catgatgg
//
LOCUS       DAOSIM010000330         1866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_53805_length_1866_cov_6.605743, whole genome shotgun sequence.
ACCESSION   DAOSIM010000330 DAOSIM010000000
VERSION     DAOSIM010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53805_length_1866_cov_6.605743"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>616)
                     /locus_tag="PLR47_10555"
     CDS             complement(<1..>616)
                     /locus_tag="PLR47_10555"
                     /inference="COORDINATES: protein motif:HMM:NF039283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5911 domain-containing protein"
                     /protein_id="HOG13434.1"
                     /translation="IDERGEIVWSCLPRFDGDPVFCSLLREHADGEGAGYCAVELIDQ
                     AESSQSYLPNTAVLVTRLTDRHGASIEITDFAPRFHQYGRTFMPMMMIRRIRRIHGTP
                     RICVRIRPVCEYGGQSCTVTHGSHHIRYVAPSWILRLTTDISVTAVLQEIPFFLETAA
                     TLILGPDETIPDAIGDLSRRFLDETIGSWRNWVRDLSIPFEWQEE"
     assembly_gap    617..626
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <627..882
                     /locus_tag="PLR47_10560"
     CDS             <627..882
                     /locus_tag="PLR47_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13435.1"
                     /translation="PMRQLPIRPSSREFFIKRFPYLRLSIFFHRKSLNGGMPEAIREQ
                     RRTVGIGQFENSVWLEAKDLLIGVLSIGKAAGDVNDESIP"
     gene            976..1485
                     /locus_tag="PLR47_10565"
     CDS             976..1485
                     /locus_tag="PLR47_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13436.1"
                     /translation="MRKPGMVSAALLAAVLAGLSAGCSGKLLKDMGSFKPSVEATENF
                     KKFVVRSDYAYFISGSDVYPLAIFGLQKDWIIDSDEDLWKKIEPKPEVMADLVTNMQR
                     RLNECCYQSPHGLDILDNQGRKIGEWYSMLGLIIGIKIKEGGKVVIYPPSDTDDVKKY
                     QDRTSGKGR"
     gene            complement(1488..>1866)
                     /locus_tag="PLR47_10570"
     CDS             complement(1488..>1866)
                     /locus_tag="PLR47_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG13437.1"
                     /translation="ACLETSIKYVKERTAFGAPLGAFQITQMKLTEMAIRIEALRGLV
                     YKAAWLIDQGKPDYTLAAMAKFYGGQTAVFCANYAVELHGGYGYIEEYPVQKWYRDAK
                     ILELYEGTKEAEIMTIGRALQAR"
BASE COUNT          428 a          497 c          539 g          392 t
ORIGIN      
        1 ctcttcctgc cactcgaagg gaatggaaag gtctctgacc cagttgcgcc agcttccgat
       61 ggtttcatcc aggaaacggc gcgacagatc gccgatggcg tccggaatgg tttcgtccgg
      121 ccccagaatc agcgtggcgg cggtttccag gaaaaagggg atttcctgaa gcacggcggt
      181 tacggaaatg tcggtggtca ggcgtaaaat ccaggacggc gcgacgtagc ggatgtgatg
      241 actgccgtgg gtgacggtgc agctctggcc gccgtattcg cacacggggc ggatgcgcac
      301 gcaaatgcgc ggcgtcccgt gaatacgccg gattcggcga atcatcatca tcggcatgaa
      361 cgtccggccg tactgatgga accggggagc aaaatccgtg atttcgatgg acgcgccgtg
      421 ccggtccgtg agccgggtca cgagaacggc cgtattgggc agataggact ggctggactc
      481 cgcctgatcg atcagctcca ccgcgcaata gccggcccct tctccatcgg catgttcgcg
      541 caaaagagag cagaaaaccg ggtctccgtc gaaccggggc agacagctcc agacgatttc
      601 accgcgctca tcaatcnnnn nnnnnngccg atgcggcagt tgccgatcag gccgagctca
      661 agggaatttt tcatcaaacg ttttccttat ttgaggttgt ccattttttt ccatcgcaaa
      721 tcactcaacg gaggtatgcc ggaagcgatc agggagcaac ggcggacagt cggtatcggt
      781 caattcgaga acagcgtttg gttagaggcg aaagatttgc tcataggcgt cttgagtata
      841 ggaaaagccg ccggggatgt caatgatgaa tcgattccgt gaaagccgcc gcggcaatcc
      901 gaataaaatg attcaaaatt tcattttacg gtttgttttt ttgtgataga aaaaacaaaa
      961 acagggagcg taaacatgcg taaaccggga atggtctcag cggccctgct ggccgcggtg
     1021 ctcgcggggc tttcggcggg ctgctcggga aagctgctca aagacatggg cagcttcaag
     1081 ccgagcgtcg aggcgacgga aaatttcaaa aagtttgttg tccggagcga ctatgcctat
     1141 ttcatcagcg ggtcggacgt ttatcccctt gccattttcg gccttcagaa agattggatc
     1201 atcgacagcg atgaggacct gtggaaaaaa atcgagccga agccggaggt catggccgac
     1261 ctcgtcacca acatgcagag gcgactcaac gagtgctgct atcagagccc ccacggcctt
     1321 gacattctgg acaaccaggg ccggaaaatc ggcgagtggt actccatgct gggactcatc
     1381 atcggcatca aaataaaaga agggggaaag gtggtcatct atcccccttc cgatacggac
     1441 gacgttaaaa aataccagga cagaaccagc ggcaagggcc ggtaagatta ccttgcctgc
     1501 agcgccctgc cgatggtcat gatttcggct tccttggtcc cttcgtaaag ctccaggatt
     1561 tttgcatccc ggtaccattt ctgcacgggg tactcttcaa tgtatccata gccgccgtgc
     1621 agctccacgg cgtaattcgc gcagaagacg gcggtctgcc cgccgtagaa tttagccatg
     1681 gccgcaagcg tgtaatcggg tttcccctga tcgatcagcc acgccgcctt gtacaccagg
     1741 ccgcgcagcg cttctatgcg gatggccatt tccgtaagtt tcatctgggt gatctgaaaa
     1801 gcgcccagcg gagcgccgaa agcggttctt tccttgacat acttgatgct ggtttccagg
     1861 cacgcc
//
LOCUS       DAOSIM010000331         1863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_53915_length_1863_cov_5.446350, whole genome shotgun sequence.
ACCESSION   DAOSIM010000331 DAOSIM010000000
VERSION     DAOSIM010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53915_length_1863_cov_5.446350"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLR47_10575"
     CDS             complement(<1..399)
                     /locus_tag="PLR47_10575"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG13438.1"
                     /translation="MDPRVCGLRDAVKRGWYRLETGELFAGFQVAQEDVALDVGCGEG
                     NATLFCARQGAHVVFSDSDAEKIKALAEKMKETNARKFEAFVSDSLPLPLPDAYATKI
                     LCTEMLEHTPEPEKIMAELVRVGKPNARYLI"
     gene            complement(611..1141)
                     /locus_tag="PLR47_10580"
     CDS             complement(611..1141)
                     /locus_tag="PLR47_10580"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOG13439.1"
                     /translation="MKPRGLLMIEHRLIEKVLLVAQQKALSMTEMDYHPAFVEAIVDF
                     IKTYADRTHHGKEEDILFTELAKKKLDDDHLRIMKELIDEHRQARAKVKEIVELNEKF
                     KKGDRKVVPAITSAVIWLAGFYPAHIRKEDAVFFPGTEPQFRQDELEDLLNRFYEFDR
                     MMIHEKYQKMYQSIKS"
     gene            1447..1725
                     /locus_tag="PLR47_10585"
     CDS             1447..1725
                     /locus_tag="PLR47_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13440.1"
                     /translation="MEKEYQIGTFCKDIKCRKHKALEGLSGNAYLRKKNVHCKDCEAW
                     KFFNWSKEKKFRIVHSLPQMSSKELAARLRGLDVVRVEDLTEDEIMCL"
BASE COUNT          427 a          542 c          459 g          435 t
ORIGIN      
        1 gatcagatac cgggcgttcg gcttgcccac ccggaccagc tccgccataa ttttctcggg
       61 ttccggcgtg tgctccagca tttccgtgca caggattttc gtcgcgtatg catccggcag
      121 cggcagcggc agcgaatcac tgacgaacgc ctcaaatttt ctggcgttgg tttccttcat
      181 tttctccgca agcgccttga ttttctcggc atccgaatcg gaaaaaacga catgcgcccc
      241 ctgacgggcg caaaacagcg tggcgttgcc ttccccgcac cccacgtcca gcgccacatc
      301 ctcctgggca acctgaaagc ccgcaaacag ctctccggtc tccagccggt accagccgcg
      361 cttcaccgcg tcccgcaggc cgcagacgcg gggatccacg tccttgggaa ccgccgtggt
      421 tccgcgggct ctccccggcc tcttgccggc gtcggatcca ggccgcggcc ggcctcgttt
      481 tcccgatggg ctatgatctt tgtccaaagg cggttttctc cccgcgaaac atattccgtt
      541 ccatcctgtc catcattggc cgtctgaatc gtttcgcgcc gactgacgga cccggaacag
      601 cgcttcgtcc tcaggatttg atggactgat acattttctg atacttttca tgaatcatca
      661 tgcgatcaaa ttcataaaac cggttgagca ggtcctccaa ctcgtcctgc cggaactgcg
      721 gctccgtgcc gggaaaaaac acggcatctt ccttacgaat gtgggccggg taaaaccccg
      781 ccagccagat cacggcggac gtaatcgccg ggacgacctt tcgatcgcct tttttgaatt
      841 tttcgttcag ctcgacgatt tcctttacct ttgcccttgc ctgtcgatgc tcatcgatta
      901 attccttcat gatgcgcaga tggtcgtcat cgagcttttt cttcgccagt tccgtaaaca
      961 ggatatcttc ctcttttccg tgatgtgttc tgtccgcata ggttttgatg aaatcgacga
     1021 tggcttcaac aaaagccgga tgatagtcca tctccgtcat ggacaaggcc ttttgctgcg
     1081 ccaccaacaa tactttttcg atcaatcgat gttcgatcat caggagtccg cgcggtttca
     1141 ttgctttcac ctccatgaaa acgatttgtc tcaaatttga agaaaaggcg acttttcaac
     1201 atcctccccc tcatccacgg caggccagat gggtcttccg cgtgatggat tcggggaacc
     1261 ctggattgtc gtgtagcaca aaaacgccac agggcaaaac aggatttttg agtattcgaa
     1321 ctaacgcttg acaagaaaaa tcataccgcc tataaaattc actacaacaa gggcgccgtc
     1381 aaaaatacgg cccatcaacc tgtcggggac ggccgccgcc ggccccggag aaaagaggga
     1441 tttcgcatgg agaaagaata ccagatcgga accttttgca aagacatcaa gtgccgaaaa
     1501 cacaaggccc tcgagggact cagcggaaat gcctatctca ggaaaaagaa cgtgcactgc
     1561 aaggattgtg aagcctggaa atttttcaac tggtccaagg aaaagaaatt ccgaattgtt
     1621 cattccctgc cccaaatgtc gagcaaagaa ctcgccgcaa ggctcagagg cctggacgtc
     1681 gttcgggttg aggatttgac ggaagacgaa atcatgtgcc tgtagaaagg cggtctgtgc
     1741 gtttccacct gtcccttcag cgccatgagt ctggccgaaa gaaaacaggt cccggccatt
     1801 cccgcgacaa cgcaggatat ggcgattcag gttcttcagg aaaagggaag actggaagcc
     1861 ttt
//
LOCUS       DAOSIM010000332         1858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_54125_length_1858_cov_7.703272, whole genome shotgun sequence.
ACCESSION   DAOSIM010000332 DAOSIM010000000
VERSION     DAOSIM010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54125_length_1858_cov_7.703272"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..1041
                     /locus_tag="PLR47_10590"
     CDS             241..1041
                     /locus_tag="PLR47_10590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG13441.1"
                     /translation="MEKIPLSVAIITKNEEKNLRGCLQSVAFAAQIVVVDSASSDATP
                     AIAAEFGCDFYTESWHGFGPQKQIAIDRCSRPWILVLDADERIPPETADVIRKIVTDP
                     ETRANGFSFPRKNYFAGRWIRHAGWWPDRVVRLFRRQAGRMTAAAVHEAVEVDGRIVE
                     LDVPIEHCTESNLGRILQKIDKYSTLGAGEAFAAGKRSSPAAAFFRAFFTFLQGYILR
                     LGFLDGPQGLTLAVTDSVNKYFKYAKLSELKRAGRRETRNQSSLRPDA"
     gene            1051..>1858
                     /locus_tag="PLR47_10595"
     CDS             1051..>1858
                     /locus_tag="PLR47_10595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG13442.1"
                     /translation="MEISFIIVNWNTKDLLRGCLDSIAKTVGSLSHEIIVVDNASSDG
                     SADMLARDYPRVQCIANAENRGFGAANNQGFAVMKGKYALLINTDAVLTEGAVEKLWR
                     FAEAHPRAAIVCGQLLNADGSKQNSVAAFPTLLSLCMNTSLLEYLFPGRYPSKRYEHR
                     EPLEIESAIGACMMVRGQALQEVSYFDERYFFFFEETDLAFAMKQAGWAVYQVPDAHI
                     YHFQGQSIGHNTQSRIEFYRSRYQFLRKWHGTAYYHAAAAVIFLRLLADVL"
BASE COUNT          405 a          542 c          510 g          401 t
ORIGIN      
        1 cgcggagttt tccggtcagt tccgaggcga actgctctgc gccgcccgcc agcccgtatt
       61 taggaatcac caccgcgatt ttgctcataa aataaattta tttcacctga attctttatg
      121 acgtgagaac taacacagat aaagcaaaaa atcattggct aatatagggt ttgcgtcggc
      181 gccggaattg tgctttatag aggcgggcaa acaaaaatct tcgaaatatt caggtattgg
      241 atggaaaaaa ttcccctgtc cgtggccatc atcacgaaaa acgaagaaaa aaaccttcgc
      301 ggctgcctgc aaagcgtcgc ctttgccgcc cagatcgtcg tggtcgattc cgcgagcagc
      361 gatgcgacgc ccgcgattgc cgcggagttc ggatgcgact tttacaccga atcctggcac
      421 ggtttcggcc cgcaaaagca gatcgccatc gaccggtgca gccggccctg gatccttgtg
      481 ctggacgccg acgagcggat tccaccggaa acggccgacg tgatccggaa gattgtgacg
      541 gatccggaga cgcgggcaaa cggcttcagt tttccgcgca aaaactattt tgcgggccgc
      601 tggatccggc atgccggctg gtggcccgac cgcgtcgtcc gcctcttccg cagacaggcg
      661 ggacggatga ctgccgccgc agttcatgaa gccgtcgaag tggacggccg gatcgttgag
      721 ctggacgtcc ccatcgagca ttgcaccgaa agcaacctcg gcaggatttt gcagaaaata
      781 gacaagtact cgacgctggg cgccggggag gccttcgccg ccggaaagcg ctcgtcgccc
      841 gccgccgctt tttttcgggc ttttttcacc tttctgcagg gatatatttt gcgcctcgga
      901 tttctggacg gaccccaggg cctcacgctg gccgtcaccg attcggtcaa caaatatttc
      961 aaatacgcca aactgagcga gctaaaaagg gcaggacgcc gtgagacgcg caatcaatcg
     1021 tccctccgcc cggatgcgtg aaattcagca atggaaattt ctttcattat cgtcaactgg
     1081 aacacaaaag accttctgcg cggctgcctg gattccatcg ccaaaaccgt cggttcgctt
     1141 tcccatgaaa tcatcgtcgt ggacaacgcc tcttcggacg gctcggcgga catgctggcg
     1201 cgggattatc cgcgggtcca atgcattgcc aacgcggaaa accgcggatt cggcgccgcc
     1261 aacaaccagg gctttgccgt catgaagggc aaatacgccc tgctgatcaa cacggacgcc
     1321 gttctgaccg aaggcgcggt ggagaagctg tggcgctttg ccgaggcgca tccccgcgcg
     1381 gcgattgtct gcggccagct cctcaatgcc gacggcagca aacagaattc cgtcgccgct
     1441 ttccccacgc tgctttcgct ttgtatgaac acgtcattgc tcgaatatct ttttcccggc
     1501 cgctacccca gcaagcgcta cgaacacaga gagccgctgg aaatcgagtc cgccatcggc
     1561 gcctgcatga tggtgcgcgg tcaggcattg caggaagttt cttatttcga cgagcgttat
     1621 ttcttctttt ttgaagagac ggacctcgcc tttgcgatga agcaggcggg gtgggctgtt
     1681 tatcaggtgc ccgatgcgca catctatcat tttcagggac agagcatcgg ccacaacaca
     1741 caatcacgaa tcgaatttta ccggtcgcgc taccagtttc tgcgcaagtg gcacggcaca
     1801 gcatattatc acgccgccgc ggctgttatc tttttgaggc tgctcgcgga tgtcctgc
//
LOCUS       DAOSIM010000333         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_56121_length_1809_cov_5.264538, whole genome shotgun sequence.
ACCESSION   DAOSIM010000333 DAOSIM010000000
VERSION     DAOSIM010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56121_length_1809_cov_5.264538"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PLR47_10600"
     CDS             complement(<1..522)
                     /locus_tag="PLR47_10600"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HOG13443.1"
                     /translation="MPAEEKIEQPDYIGHRQRLKARFEKTGFYGFQDYEVLEFLLYFI
                     LPRKDTKPIAKKLLRRFKNFIGVIHADTRELEKIEGIGAHAALFLKASGATIGFYFDE
                     RAKQADFQFTNLDQLVDYFRAAIGGKPNEILRALYLNSRNGLISAENLSEGTVSEAVA
                     FPRKIVEGALKYGA"
     gene            complement(501..1175)
                     /locus_tag="PLR47_10605"
     CDS             complement(501..1175)
                     /locus_tag="PLR47_10605"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOG13444.1"
                     /translation="MRYVYGFNPIIVQALGYGNQVHNLLNLLHKIAQQTGQVPLEEEA
                     AKLLQEHFSLRYAAREQEETLKRSALRSILRYLNLWREDFSLSVKTERTFEMDLDNAL
                     LTGTIDLLKRENGDSNILEIIDFKTGNDRKDRDALDLQVQLYTLAAREALDLNVQKAY
                     VHFLDDQKQPRLEVLTTPKQLDLAMRTLADAVQGITTRRFQRNPRSKKSCITCDWEKI
                     CPQKKK"
BASE COUNT          402 a          445 c          468 g          494 t
ORIGIN      
        1 agccccatat ttaagtgcgc cttcgacgat ttttcttgga aaggcgacgg cctccgagac
       61 ggtcccttcg ctcagattct ctgcggatat caggccgttt ctgctgttca gataaagggc
      121 acgcaggatt tcgtttggtt tcccgccaat ggctgcccga aaataatcaa ccagttgatc
      181 cagattggta aactggaaat ccgcctgctt ggccctttca tcgaaataaa agccaatggt
      241 tgcgccgctt gcttttaaga atagcgcggc atgtgcaccg atgccttcga ttttttccag
      301 ttcccgcgta tcggcatgaa tgacgcctat gaagtttttg aaccttctca aaagtttctt
      361 ggcaatcggc tttgtatctt tccggggaag aataaaataa agcagaaatt ccaaaacctc
      421 ataatcttga aagccataaa agccggtttt ttcaaagcgc gctttaagcc tttgtcgatg
      481 accgatatag tccggctgtt ctattttttc ttctgcgggc atattttctc ccagtcgcag
      541 gtgatgcatg actttttact gcgcggattt ctttgaaaac ggcgcgttgt aatgccttga
      601 acggcatcag cgagtgttcg catcgcaaga tccaactgct ttggtgtcgt cagtacctcc
      661 aaccgcggct gcttttggtc atcaagaaaa tggacgtaag ccttttgcac gttgagatcc
      721 aaagcttctc gcgctgccag tgtataaagc tggacttgga gatccaatgc atcccgatcc
      781 ttgcggtcgt ttccggtctt gaaatcgata atttccagaa tgttactgtc tccgttttcc
      841 cgtttgagca gatcgattgt gccggtaagg agagcgttgt cgagatccat ttcgaatgtg
      901 cgctccgttt tgacggaaag ggagaagtct tcccgccaaa ggttcaaata acggaggatg
      961 ctgcgcagag cgcttctttt tagggtttcc tcctgttctc tcgccgcata acgaagggaa
     1021 aaatgctcct gcaaaagctt tgccgcttct tcttccagag gcacctggcc ggtctgctgc
     1081 gcaattttat gaagcaagtt caaaagatta tggacctggt tgccgtagcc cagggcctgg
     1141 acgatgattg gattgaaccc gtaaacatag cgcattttgt agtcatattc gcaccggatg
     1201 tagtcgctca gttccgagta gctggtgggg aagcggtatt cctccagcga aggttccgag
     1261 ggcaggcgct ttctttttgt cggatcggca gtatgagccg ttatgcagca ttcgtcagcc
     1321 agttcggaaa agtgttgcag cggccttttc gccttgacct ttcctacagg ctgccgggat
     1381 gttgtcacaa agaggaattt tttcgcccgc gtaaggccta cataaaacag acggcgttca
     1441 tcttcgacgc ttccgctgta ccgagcaaag ttgaatgttt ttggatcaag gaatcctggg
     1501 ggggtttgcc ggtgaggccg ctcaatgcaa tagggcatga aaacgacggg aaagccaagg
     1561 cctttggcac cgtgcagcgt catgatctgg acggcgttga tgaagcgagt tggatcttcg
     1621 cccgcgcccg cgtcataagc tccctcggcg tagtggcgga tgaaccagca gaagcgctcg
     1681 atgtccttga aggtgcagta gcttcgggtt ccttcataat cagagattgc ctgactcagg
     1741 cggcccagat tgtacatctc gatctcgtgc tcatcggtgt ggtattctcc atcatcaaca
     1801 ccgagaagc
//
LOCUS       DAOSIM010000334         1791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_56915_length_1791_cov_8.057028, whole genome shotgun sequence.
ACCESSION   DAOSIM010000334 DAOSIM010000000
VERSION     DAOSIM010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56915_length_1791_cov_8.057028"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PLR47_10610"
     CDS             complement(<1..618)
                     /locus_tag="PLR47_10610"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOG13445.1"
                     /translation="MELKDIQKVLILGAGAMGQQIGFVCAMHGYRVTYYDISDEILKA
                     SMKRVEKLGSAFFVPVGRITMEQLHEIMGRISATADPAQAAKDADLVSESVIEDPKIK
                     AEVFARFNKLCPERTIFTTNTSLLMPSKFAAETGRPEKFLALHFHDLRANNVVDVMPH
                     PGTDPAVTQLVREFAVSIGQIAIMLCRESNGYVFNAMLSSICTAAM"
     gene            841..1182
                     /locus_tag="PLR47_10615"
     CDS             841..1182
                     /locus_tag="PLR47_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13446.1"
                     /translation="MRSLFNIIRVLWLFLWAGIATIVLAVPIISAGLLSRTGNLGFSL
                     SKIWAWTMLAVSFVRKGSLVVRPGKIQVVVGEPIDTGSYTHENVDALIDKTRRTVIAN
                     FDPGYAGRFFA"
     gene            1243..>1791
                     /locus_tag="PLR47_10620"
     CDS             1243..>1791
                     /locus_tag="PLR47_10620"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOG13447.1"
                     /translation="MPNVFFEAAAGKRKPYVDSLRKLLDQFCSPFDSGDSVGIKLHWG
                     ERGNKGYLPCDYAREIVAWLRARKVEPFIFDTTVLYSGGRRTAADSLKTAADHGYTSD
                     FLKCPVIIADGADGRNVVSLPAGYRHFKTVQVASVFEQAKGFVIFSHFKGHMVSLFGG
                     AIKNISMGFASRAQKQRMHADYH"
BASE COUNT          348 a          526 c          498 g          419 t
ORIGIN      
        1 catggcggcc gtgcagatgc tggagagcat ggcgttgaac acgtagccgt tgctttcccg
       61 gcaaagcatg atggcgatct ggccgatgct cacggcaaac tcgcgcacga gctgggtcac
      121 cgccgggtcc gttcccgggt gcggcatgac gtccacgaca ttgttggccc tgagatcgtg
      181 aaagtggagc gccagaaatt tttctggcct gccggtttcc gcggcgaatt ttgagggcat
      241 gaggagcgat gtgttggtgg taaagatcgt ccgctccggg cacagcttgt tgaaccgggc
      301 gaaaacctcc gccttgatct tcgggtcctc aatcacggat tcgctgacca ggtccgcgtc
      361 tttggccgct tgggccgggt cggcggtcgc cgagatgcgg cccatgatct cgtggagctg
      421 ctccatcgtg attctgccca cgggaacgaa aaaggcggag ccgagttttt ccacgcgctt
      481 catggatgcc ttcaaaattt cgtcggaaat gtcgtagtag gtcacgcggt agccgtgcat
      541 ggcgcaaacg aagccgatct gctggcccat ggcgcccgcg cccagaatca gcactttttg
      601 aatgtctttc agttccataa attttccctt tccctgatgc aaatttttct gcgccggatt
      661 cctatagcac aggtttgtcc ggagttcagc ccttttcgcg gccgtttcac gacacggaat
      721 ttcccgcgca ggattttctt gacatccccg ggcgcgttca ctatgattcg cacacaataa
      781 ggatcagcaa tcagccaaaa ccggcggccg catacccgaa tcatcatgag gatggactgg
      841 atgcgctctc ttttcaacat catccgtgtt ttgtggcttt tcctgtgggc aggcatcgcc
      901 accatcgtcc tcgcggttcc catcatctcc gcggggctct taagccgcac cgggaatctc
      961 ggtttttccc tctcgaagat ctgggcctgg accatgctgg ctgtttcttt tgtccgcaag
     1021 ggcagcctgg tcgtgcggcc cggaaagatt caggtggtcg tgggcgaacc catcgatacc
     1081 ggaagctata cccacgagaa cgtggacgcg ctgatcgaca aaacgcgccg gaccgtcatc
     1141 gcgaatttcg atcccggcta tgccgggcga ttcttcgcct agcgggcaat tccgcccgac
     1201 gtttcgcgcc ggcatcgccg gcgaaggaca gagaccctga tcatgccgaa tgtctttttt
     1261 gaagccgcgg ccggcaaaag aaaaccttat gtcgattcac tgcggaaact gctggaccag
     1321 ttttgctcgc cgtttgattc aggcgattcc gtcggcatca agctccactg gggcgagcgg
     1381 ggcaacaaag gctacctgcc ttgcgactac gcaagggaaa tcgttgcctg gcttcgcgct
     1441 cgaaaagtgg agccgtttat tttcgacacc accgtccttt actccggcgg gcgccgcacc
     1501 gccgcggatt cgctgaaaac cgccgccgat cacggctaca cgtccgactt tctcaaatgt
     1561 ccggtcatta ttgccgacgg cgcggacgga agaaatgtcg tctctcttcc cgcgggctac
     1621 cgacacttca aaaccgttca ggtcgcatcc gtttttgaac aggcaaaagg ctttgtgatt
     1681 ttttcccatt tcaagggaca tatggtctct ttgtttggcg gcgccatcaa aaacatttcc
     1741 atgggatttg cctcccgcgc ccagaaacag cggatgcatg ccgattacca t
//
LOCUS       DAOSIM010000335         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_56955_length_1790_cov_7.779827, whole genome shotgun sequence.
ACCESSION   DAOSIM010000335 DAOSIM010000000
VERSION     DAOSIM010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56955_length_1790_cov_7.779827"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PLR47_10625"
     CDS             complement(<1..747)
                     /locus_tag="PLR47_10625"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG13448.1"
                     /translation="MDFALTEEQKMIRETARNFAEKEIAPHVEEDEKNHFWRREIFDK
                     MAELGFFGFCIDEKYGGNGMGFLEGALAIEQVARVHVAWRMAFNMQCWGPALTIQKFG
                     TEEQKERYIPKFVSGEYVGSFAMTEPDIGSDVASMKCRAEDKGDYYLVNGNKMWITNG
                     TVCNHGLLYVKTDKDAGANGVSCFIMDYSLPGITRKPIHDKVGLWASDTAEITFEDVK
                     VPKNLLLGKLNKGFQICMTQLNSTRLGCSAG"
     gene            complement(941..>1790)
                     /locus_tag="PLR47_10630"
     CDS             complement(941..>1790)
                     /locus_tag="PLR47_10630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOG13449.1"
                     /translation="KYGFMPRGHGALAMWGIPFYDRIQRGRVMSQPIWRYGELSSMMT
                     WAEAAAKKEGISREEADRWALRSHQRAIAAQDAGKFTDEIAPVLVGKDKSGQEIYFKT
                     DEGPRRETTLEKLAKLKAVYKDGVCTAGNSSSENDGAAVVILASEKKAKELGIKPIAY
                     YRSCAVAATDPTLTYPAVPAAVNKALAKIGKAINDMDLIEVQEAFAVQCLADSRLAGL
                     SDEQMDAKVNINGSGISLGHPIGATGTMRLTTLLNEMQRSNARFALETICGGGGQGIC
                     MVIERT"
BASE COUNT          302 a          540 c          505 g          443 t
ORIGIN      
        1 gcccgcggag cagcccagcc gggtggagtt gagctgcgtc atgcaaatct ggaatccctt
       61 gttcagtttt cccagcaaaa gatttttggg gactttgacg tcttcaaacg tgatttccgc
      121 cgtgtcggag gcccacaggc cgactttgtc gtgaatgggt tttctcgtaa tgccgggcag
      181 ggagtagtcc atgatgaagc agctcacgcc gtttgcgccc gcgtccttgt ctgttttcac
      241 gtacagcagg ccgtggttgc aaaccgtgcc gttggtgatc cacatcttgt tgccgttcac
      301 caggtagtag tcgcccttgt cctcggcgcg gcatttcatg gaggcgacgt ccgagccgat
      361 gtccggttcg gtcatggcaa agctgccgac gtattcgccg gagacgaatt tgggaatgta
      421 gcgctctttc tgttcctcgg ttccgaactt ctgaatggtg agcgccgggc cccagcactg
      481 catgttgaag gccatgcgcc aggcgacatg cacgcgggcc acctgttcga tggcgagcgc
      541 gccttccaga aagcccatgc cgttcccgcc gtatttttcg tcgatgcaaa agccgaaaaa
      601 ccccagctcc gccatcttgt caaaaatttc cctgcgccag aaatggtttt tctcgtcttc
      661 ttcaacgtgc ggcgcgattt ccttttcggc aaaattgcgc gccgtctcgc gaatcatttt
      721 ttgttcttcc gtcaatgcaa aatccatatc ccttttcctc ccggccgttg ttagtgctta
      781 ttctgatctt cgtcattccg gcgaaggccg gaatccagtg cgtgccttca ggcgcgcgtt
      841 ggcgggtctg aagacccgcc ctacattcac cactttctta atggttggct acattcgatc
      901 cttttcatgt aaggaacggt cgcgaccgtt ccttacggtg ttatgttcgt tcgatcacca
      961 tgcaaatccc ctgtcctccg ccgccgcaga tcgtttccag ggcgaagcgg gcgttgctcc
     1021 gctgcatttc gttcagcagc gtggtgagcc gcatcgtgcc cgtggcgccg atagggtgcc
     1081 cgagcgaaat gcccgaaccg ttgatgttga ccttcgcgtc catctgctcg tcggaaagtc
     1141 ccgccagacg cgaatccgcc agacactgaa cggcaaaagc ctcctgcact tcgatcaggt
     1201 ccatgtcgtt gatggccttg ccgatctttg ccagcgcctt gttgacggcc gccggaacgg
     1261 ccgggtacgt gagcgtcggg tcggtggcgg caaccgcgca ggaccggtag taggcgatcg
     1321 gtttaatgcc cagttccttc gccttctttt cagaagcgag gatcaccacg gccgcgccgt
     1381 cgttttccga agaggagttg ccggccgtgc agacgccgtc cttgtaaacg gctttgagtt
     1441 tggccagctt ctccagcgtg gtttcgcggc gggggccttc atcggttttg aaataaatct
     1501 cctggccgct tttgtccttg cctacaagca ccggagcgat ttcatcggtg aatttccccg
     1561 catcctgcgc cgcgatcgca cgctgatgcg atcgaagcgc ccagcggtcc gcttcttcgc
     1621 gcgagatgcc ttcttttttg gctgcggctt ccgcccaggt catcatggag ctcagttctc
     1681 cgtagcgcca gatgggctgg ctcatgacgc ggccgcgctg aatgcggtcg taaaacggaa
     1741 tgccccacat ggcgagcgcc ccgtggccgc gcggcatgaa gccgtatttc
//
LOCUS       DAOSIM010000336         1774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_57705_length_1774_cov_12.288540, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000336 DAOSIM010000000
VERSION     DAOSIM010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57705_length_1774_cov_12.288540"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /locus_tag="PLR47_10635"
     CDS             complement(<1..1370)
                     /locus_tag="PLR47_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG13450.1"
                     /translation="MEQPWFKQYAKHVSRTLQYPNAPVYAFLEKTAAAHPEYIAMTFN
                     DADTTYAELARKVNRFAAVLNGQGVKKGDRVALILINSPTYVISFFAILKLGAVAVNL
                     SVGITGEELAACLINSGARMAITLDLFAKNLYRVISKTSVKNVILHSVFGMEKKIPLT
                     EGMPAPQIFSDLMAGAGDAAGPDVEVLTTDVAVLQYTSGSTGAPKAATLTHANLVAST
                     TQSDKWMGHEHTGNAAVLCVIPFFHVFGMTAGLLIPVSKGYRMLLLPRMDAMDILSMT
                     KMLETHRPISFPAVPSLWQAIVLLPDETARDYLSSILVATSGGAPAPAALHDRYKSLT
                     GKRMIEAYGLSEASSATHMSPYPKGGPVGSVGVPLPDTQAKIMDLETGQRECEPGEIG
                     ELVVRGPQIMRGYWNNPELTAAVLRDGWFCTRDLARMDENGFFYIIDRKDDMIISRGF
                     NVYPAQ"
     gene            complement(1395..>1774)
                     /locus_tag="PLR47_10640"
     CDS             complement(1395..>1774)
                     /locus_tag="PLR47_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13451.1"
                     /translation="PLDFWGTYLVNDFLFEALNAAYEDDSIHMVVVDRLIPRIAFHSP
                     DLTSDSFAEIVEFIQSRRQAKPTVFTIDYDGGDSDLIRQGSDLRARFCRAGIPAYPSF
                     ERAIGALARFYRYCRRFGHAAGR"
BASE COUNT          373 a          514 c          525 g          362 t
ORIGIN      
        1 atctgggcgg gatagacgtt gaatcccctg gaaatgatca tgtcgtcctt gcggtcgatg
       61 atatagaaaa atccgttttc gtccatgcgg gcaaggtcgc gcgtgcagaa ccacccgtcg
      121 cgcaaaacgg cggcggtgag ctccggattg ttccagtagc cccgcatgat ctgcggcccc
      181 ctgaccacca gctccccgat ctcacccggt tcacattccc tttgaccggt ttccagatcc
      241 atgattttcg cctgcgtgtc cggaaggggc acgccgaccg atcccaccgg cccgcctttc
      301 ggatagggcg acatgtgcgt cgcggaggac gcttcggaaa ggccgtaggc ttcgatcatc
      361 cgctttccgg tcaggctttt gtagcggtca tggagcgcgg ccggcgcggg cgctccgccg
      421 ctggtggcca ccagaatgga ggaaaggtag tcccgggccg tctcatcggg aagcaaaacg
      481 atcgcctgcc agagactggg caccgcagga aacgatatcg ggcggtgggt ttccagcatc
      541 ttcgtcatgg acaggatgtc catcgcgtcc attcggggaa gcagcagcat tcggtatccc
      601 ttgctcaccg gaatcagcag gccggccgtc attccaaaga cgtgaaaaaa tggaatgacg
      661 cacagcaccg ccgcgttccc cgtgtgttcg tggcccatcc atttgtccga ttgcgtcgtg
      721 ctggcaacaa gattggcatg cgtgagcgtg gccgccttgg gtgcgcccgt ggagccgctg
      781 gtgtattgca gcacggcgac atccgtggtt aagacctcaa cgtccggccc ggccgcatcg
      841 cctgcgccgg ccatcaaatc ggaaaagatc tgcggggcgg gcatgccctc cgtcagggga
      901 atctttttct ccattccgaa cacggaatga agaatgacgt ttttcacaga ggttttggaa
      961 atcacccggt agagattttt ggcaaacaga tccagcgtaa tggccattct cgcgccggaa
     1021 ttgatgaggc aggcggccag ttcctcgccc gtgatgccga cgcttaagtt gaccgcgacc
     1081 gcgccgagtt tcagaatcgc gaaaaaagaa atgacgtagg taggcgaatt gatcagaatc
     1141 aaagcgacgc ggtctccttt tttcacgccc tgcccgttta gaacggcggc gaaccggttg
     1201 accttccggg caagttcggc gtaggtcgta tccgcgtcgt tgaacgtcat ggcgatatat
     1261 tccggatgcg ccgccgccgt cttttccaga aatgcataaa ccggcgcatt cggatactga
     1321 agcgtccggg acacatgctt cgcgtattgt ttgaaccagg gctgttccat gttttcttcc
     1381 tttccaaaac gggactatcg gccggccgcg tggccaaagc gccggcagta tcgataaaag
     1441 cgcgccagcg cgccgatggc cctttcaaac gacgggtagg ccggaatccc cgctctgcag
     1501 aaccgggcac gcagatcgga accctgccga atgaggtcgg aatcgcctcc gtcgtaatca
     1561 atggtgaaga cggtgggctt ggcctgcctg cggctttgaa tgaactccac aatctcagca
     1621 aacgaatccg aggtcaagtc cgggctgtgg aaagcgatgc gcgggatcag gcggtcgacg
     1681 accaccatgt gaatgctatc atcctcgtag gccgcattga gcgcctcgaa aagaaaatca
     1741 ttcaccagat aggttcccca gaaatcgagg ggat
//
LOCUS       DAOSIM010000337         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_57996_length_1767_cov_8.295561, whole genome shotgun sequence.
ACCESSION   DAOSIM010000337 DAOSIM010000000
VERSION     DAOSIM010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57996_length_1767_cov_8.295561"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="PLR47_10645"
     CDS             <1..1389
                     /locus_tag="PLR47_10645"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOG13452.1"
                     /translation="AKGLEWPIVFCPFVWSDRSRSLKDEVCTYHEGDTLVLDYATDDE
                     KKKKQEDEAGEERVRLIYVALTRAANRCYIVAGPFWQPSGKGATEKKAKKSILNWIVA
                     GDEIKHGVWAEKKEVTVDSMHQAWQRLLTSNKGIGGIPWPDKKETVRPVICKEKRKYE
                     ARKVGRLPGVAWKISSYSSLTASSLEGGRQHEPATQDYDVIPLAIEDHDPPQVLRAKL
                     SGDIIDFPAGTTTGHCLHAVFERIDFQDESGWKDIITNVLANYPPVDALEKRDDQVSR
                     IHGMLKDVMSTPLPTGFKLGDVARAKRFSEFEFAYSVRHLDHAKLKNLLDKHGCRIPP
                     LDFPDLEGYLRGFIDLVFEHAGKWYVLDWKSNKLGISAAEYSRSSVDEAMFNHAYEFQ
                     GLIYLIALHRYLKLRISKYDYSRHMGGILYLFVRGVRPAWPSAGIWHKLPEPALIESL
                     DALFSSENRRTV"
     gene            1395..>1767
                     /locus_tag="PLR47_10650"
     CDS             1395..>1767
                     /locus_tag="PLR47_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13453.1"
                     /translation="MRKHENIPAADYASPLSRGLADFLKRLSLTLGATEEAARTAGDA
                     ARACAQALAEGDVCANFSLLAAAEGKPAQDVRKLLDASLVASSAGADKKLPLVYDDEG
                     QIYLYRYFAYERKLADAIIRRL"
BASE COUNT          437 a          444 c          499 g          387 t
ORIGIN      
        1 gccaaaggcc tggaatggcc gattgtcttt tgcccgttcg tctggagcga ccgcagccgg
       61 tcattgaagg atgaggtctg cacctatcac gagggcgata ccctggtgct tgattatgca
      121 accgacgacg aaaaaaagaa aaaacaggaa gacgaggccg gcgaagaacg ggtgcgactg
      181 atttatgttg cgctgacgag ggcggcaaac cgctgctata ttgtcgctgg tcctttctgg
      241 caaccgtcgg gaaagggtgc aacggaaaaa aaggcgaaga agagcatcct gaactggatc
      301 gtcgccggcg atgagataaa acacggcgtg tgggcggaaa aaaaagaggt gaccgttgat
      361 tccatgcatc aggcatggca gagactcctg acatcaaaca aagggatcgg agggattccg
      421 tggccggata aaaaagagac agtccgtccg gttatttgca aagagaagag aaaatatgaa
      481 gcccggaagg tcggcagact gccgggtgtg gcctggaaaa tatccagtta ctcatcgctt
      541 accgcctcct cccttgaagg aggaaggcaa cacgaaccgg cgacacagga ttatgacgtc
      601 atcccccttg cgattgaaga tcatgatccg cctcaagtcc tccgggcaaa gctttcgggc
      661 gatatcatcg attttccggc cggtaccaca acaggacatt gtctgcatgc cgttttcgaa
      721 aggattgact ttcaggacga aagcggatgg aaagacatca tcaccaacgt gctggcgaat
      781 tatccgccgg tggatgcctt agaaaagcgc gatgatcagg tcagccggat tcacggtatg
      841 ctgaaagatg tcatgagcac ccctctgcca accgggttta aactcggcga cgttgccagg
      901 gcaaaaagat tttccgaatt cgagttcgcc tattcggtac gtcaccttga tcacgcaaaa
      961 cttaagaatc ttctcgacaa acacggctgc cggattcccc cgctggattt tcccgatctc
     1021 gaaggttacc tgcggggttt cattgatctt gtctttgaac acgcgggcaa atggtacgta
     1081 ctggattgga aatcgaacaa gctgggcatc agtgccgcgg agtattcccg gtcgtccgtc
     1141 gatgaggcaa tgttcaatca tgcctatgaa tttcagggcc tgatctatct tatcgctctt
     1201 catcgttact tgaaactgcg tatcagcaaa tatgattaca gccggcacat gggaggcatt
     1261 ctgtatctct ttgtccgtgg cgttcgtccg gcatggcctt cggcaggtat ctggcataaa
     1321 ctgcccgagc cggcgctgat cgaatcactg gatgccctgt tctcttcaga aaaccggagg
     1381 accgtatagc gttgatgaga aaacatgaaa acatacctgc ggccgattat gcatcgcccc
     1441 tgtccagggg acttgcggat tttctgaagc gcctttcttt gacccttggt gctacggaag
     1501 aggcggcgcg cacagcgggc gatgccgcca gggcgtgcgc tcaagccctg gccgaaggcg
     1561 atgtctgcgc gaatttctct ctgctggccg cagcggaagg caagccggca caggatgtac
     1621 gaaagctgct ggatgcttct ttggtggcct catccgccgg ggcggataaa aaattgcccc
     1681 tcgtgtatga cgatgaagga caaatctacc tgtaccggta ctttgcctat gagcggaaac
     1741 tggccgatgc aattatcagg cggttac
//
LOCUS       DAOSIM010000338         1763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_58180_length_1763_cov_8.962529, whole genome shotgun sequence.
ACCESSION   DAOSIM010000338 DAOSIM010000000
VERSION     DAOSIM010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58180_length_1763_cov_8.962529"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PLR47_10655"
     CDS             complement(<1..454)
                     /locus_tag="PLR47_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13454.1"
                     /translation="MKALKGILIFAVVLICLSAVVTGINILQKGGAWDGRSMEEILGV
                     APQKATLEDIGKLDKAEIFQLYYAASAPSFHDFRGEFAAVTVDVGILASSAAYFTHHF
                     FGPGRWEGKAFNPSEKDRGYGYNIFRDAGGKVYRTRRMDTEIGPSDYDG"
     gene            complement(481..1293)
                     /locus_tag="PLR47_10660"
     CDS             complement(481..1293)
                     /locus_tag="PLR47_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG13455.1"
                     /translation="MKDLRGKIALVTGAAMGMGRSLSEDFLREGCAVALVDVNGPALA
                     EAQAELAKTGRCAAFVCDISNREAVYELARRVEKEFGPVDILVNNAGIVKARELLDLS
                     DDDIEKTIRINLTAQFWTCKAFLPSMIQRGGGHIVNFASAGGILSLPNLSAYCASKFG
                     VVGFSDSLRQEMKKHQYNIGVTYVCPNTVNTGMFEGSKMVAGTRMLSASDVTKKVIRA
                     VKRNQAMVAVPDFPVKILTPLTKVLLPIRAMDWLNKIMGMATCNDSWRGRSC"
     gene            complement(1339..>1763)
                     /locus_tag="PLR47_10665"
     CDS             complement(1339..>1763)
                     /locus_tag="PLR47_10665"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF014820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOG13456.1"
                     /translation="ASATVIMSMEKAKELGLKPLAKIKAVGYGGCDPSVMGLSPVPAV
                     RSLMKRSGLKLEDFELIEVNEAFAAQYLGCEKELKINREITNVNGSGIGLGHPVGATG
                     NRIMVTLIHAMKKRKKTLGLATLCGGGGVSIATAIEML"
BASE COUNT          347 a          549 c          469 g          398 t
ORIGIN      
        1 tgccgtcgta atccgacggt ccgatttccg tgtccattct gcgcgtccgg taaactttgc
       61 ccccggcatc cctgaaaata ttgtacccgt atcccctgtc tttttcagac gggttgaagg
      121 ccttgccctc ccagcgtccc ggaccgaaaa agtgatgggt gaaataagcc gcgctggacg
      181 ccagaatgcc gacatccacc gtcaccgcgg caaattcgcc ccggaaatcg tgaaaggaag
      241 gcgcggaagc ggcatagtaa agctggaaga tctccgcctt gtcgagcttg ccaatgtcct
      301 ccagggtcgc cttttgaggc gcaacgccca gaatctcctc catgcttctt ccgtcccagg
      361 cgccgccctt ctgaagaata ttgattccgg tcaccacggc cgacagacaa atcaaaacaa
      421 cggcaaaaat cagaattccc tttaatgctt tcatcttatt cccgtcctga aatttatttt
      481 ttaacagctc cggcctctcc acgaatcgtt gcaggtggcc atgcccatga ttttgttcaa
      541 ccagtccatc gccctgatgg gaagaagaac cttggtgagc ggcgtcagga ttttcacggg
      601 aaaatccggc accgcgacca tggcctgatt gcgcttgacg gcccggatca cctttttggt
      661 cacgtcggag gcggaaagca ttctggtgcc cgcaaccatc tttgatcctt caaacatgcc
      721 ggtattcacc gtgttggggc atacgtaggt gaccccgatg ttgtactgat gttttttcat
      781 ttcctggcgc agggaatcgg agaagcccac caccccgaat ttgcttgcgc agtaagcgga
      841 aagattggga agagacagaa tgccgcccgc cgaggcgaag ttgacgatat ggccgcctcc
      901 cctttgaatc atggagggca gaaacgcctt gcacgtccag aactgggcgg tgagattgat
      961 gcggatggtt ttttcaatgt cgtcgtcgga aagatcgagc agctcccggg ccttgacgat
     1021 gccggcgttg ttgaccagaa tatcgaccgg accgaattcc ttttccaccc ggcgggccag
     1081 ctcgtaaacc gcctccctgt tggaaatatc gcagacgaag gcggcgcacc tgcctgtttt
     1141 cgcaagctcg gcttgggcct ccgcaagcgc cggtccgttc acgtccacca gcgcgaccgc
     1201 gcatccttcc ctgaggaaat cctccgacaa actccggccc atgcccatcg ccgcgcccgt
     1261 aaccagggca attttacccc gcaaatcctt catggtcttc cctccccgat gacgggccgg
     1321 tgtttgccgc cggaccgctt acagcatttc aatggccgtt gcgatggaaa cgcctccccc
     1381 gccgcacagc gtggcgaggc ccagcgtctt tttgcgcttt ttcatcgcgt ggatgagcgt
     1441 taccatgatc cggttgccgg ttgcgccgac cggatggccc aggccgattc cggagccgtt
     1501 cacgttcgta atctcccggt tgattttcag ctctttttca catcccaggt actgggcggc
     1561 gaacgcttcg ttcacctcga tcagctcaaa atcctccagc ttcaggccgg agcgcttcat
     1621 caggcttcga accgcgggga cggggctcag tcccatgacg gacgggtcgc atccgccgta
     1681 tccgaccgcc ttgatcttgg ccaggggctt gaggcccagc tccttcgctt tctccatgga
     1741 catgatgacg gtggcgctgg ccg
//
LOCUS       DAOSIM010000339         1748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_58853_length_1748_cov_15.383343, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000339 DAOSIM010000000
VERSION     DAOSIM010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58853_length_1748_cov_15.383343"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PLR47_10670"
     CDS             <1..281
                     /locus_tag="PLR47_10670"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOG13457.1"
                     /translation="KQESRKLMKILDSRFRRNDDLEAGMAFSKGLKMPLGGAVLVKAK
                     LTDAILTDAALYDADLTAADLTRAVISHANFNFSPFFSRICRLRNCLT"
     gene            309..1481
                     /locus_tag="PLR47_10675"
     CDS             309..1481
                     /locus_tag="PLR47_10675"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG13458.1"
                     /translation="MIRRDAEDTIHALLRGFPIVTITGPRQSGKTTLAKAIFSDKPYA
                     SLEEPDVRLAAREDPRSFLARFPDGAVLDEVQRCPELLSYLQPSVDADGRMGLFLLTG
                     SQQFGLLSGITQSLAGRTAFVELLPFSLHELAQAGRRPGDMERLLFTGGYPPLYDRDV
                     AVRAWFSAYVTAYIERDVRQVLKIQELETFQRFVRLCAGRTGQLLNYSSLAADCGITH
                     NTAKAWISVLEASYIVFQLRPHHENFNKRLVKASKLYFYDVGLVSWLLGIQTPAQIET
                     HPLRGNIFETFVVAEWMKSFLNRGERPAFYFWRDSNGVEVDLLIEQGGRIMPVEIKSG
                     KTVVGEFFSGLEKWTALAGDMAVAPTLIYGGSQDFEHKGVSVRGWQKMGRCPSFPN"
     gene            complement(1568..>1748)
                     /locus_tag="PLR47_10680"
     CDS             complement(1568..>1748)
                     /locus_tag="PLR47_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13459.1"
                     /translation="FLPQMFITNSPSTKKGASSPPYPHTPSHAGHILPNFDEFVKSHD
                     GDDEVKSSRGKARES"
BASE COUNT          356 a          491 c          497 g          404 t
ORIGIN      
        1 gaaaacagga atcaaggaag ttaatgaaaa tactggattc ccgctttcgc cggaatgacg
       61 atttggaggc tggaatggcg ttttccaaag gtctcaaaat gcctttggga ggcgctgtcc
      121 ttgtaaaagc caagctcacg gatgcgatac taaccgacgc cgcattgtat gatgccgatc
      181 tgaccgccgc cgatttgacg cgggcagtta tttcccatgc caatttcaat ttttccccct
      241 ttttttcacg catatgcaga ttgagaaatt gtttgacata atacataaga tgcggtaggc
      301 tcacgcccat gatacgacgg gacgccgaag acaccatcca tgcgctgttg cgggggtttc
      361 cgattgttac catcaccggg ccgcgccagt ccgggaaaac caccctcgcc aaggccattt
      421 tctccgacaa accttacgcc tccctggaag agccggacgt gcggctggcc gcccgggagg
      481 acccccggtc gtttctggcg cggtttcccg acggcgccgt cctcgacgag gtgcagcgtt
      541 gtccggagct tctctcctat cttcagccca gcgtcgatgc cgacgggcgc atgggtctgt
      601 tccttctgac cggctcgcag caattcggcc tgttgtccgg cattacccaa tctttggcgg
      661 gaagaaccgc cttcgtggaa ttgctccctt tttcgttgca cgagctggcg caagccggcc
      721 ggcgccccgg cgatatggag cgcctgctct tcaccggcgg ctacccgccg ctctatgatc
      781 gcgacgtcgc tgtccgcgca tggtttagcg cctacgtgac ggcgtacatc gagcgggatg
      841 tccgtcaggt tcttaaaatt caagagctgg aaacctttca acgtttcgtg cgcctctgcg
      901 ccggccgcac cgggcagttg ctgaactatt cctcgctggc cgcggattgc gggattaccc
      961 acaacaccgc caaagcctgg atatcggtat tggaagccag ctacatcgtg tttcagcttc
     1021 gtccccatca tgagaatttc aacaagcggc tggtcaaggc gtccaaactc tatttttacg
     1081 acgtcggcct ggtctcctgg ctgctgggca tccagacgcc ggcgcagatc gaaacgcatc
     1141 ccctgcgggg aaacatattc gaaaccttcg tggtggcgga atggatgaaa tcatttctca
     1201 atcgcgggga gcggcccgcg ttctatttct ggcgcgacag caacggcgtg gaggtggatc
     1261 ttctcatcga gcagggggga cggatcatgc cggtcgagat caagtccgga aaaacggtgg
     1321 tgggggaatt tttctccggc ctcgaaaaat ggacggccct ggcgggggat atggcggtcg
     1381 ctcccaccct gatctacggc ggttcacagg actttgaaca caaaggggtc agcgtcaggg
     1441 gctggcagaa aatggggcgc tgtccctcat tcccgaatta aatattgacg acatcgtaaa
     1501 aagtccatat gcggcgttgc gcttcatcct tcgtcactgc gacgtacgca caagtacgcc
     1561 tcattcctca ggattcgcgc gccttgcctc tggagctttt tacttcgtcg tctccatcat
     1621 gactttttac gaattcatca aaattgggta agatgtggcc ggcatgcgac ggggtgtggg
     1681 ggtacggggg cgacgaagcc cccttttttg ttgatggaga attggtgatg aacatctgcg
     1741 ggagaaaa
//
LOCUS       DAOSIM010000340         1738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_59371_length_1738_cov_6.584670, whole genome shotgun sequence.
ACCESSION   DAOSIM010000340 DAOSIM010000000
VERSION     DAOSIM010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59371_length_1738_cov_6.584670"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PLR47_10685"
     CDS             <1..233
                     /locus_tag="PLR47_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HOG13460.1"
                     /translation="ASGNMAKLYGLNLIGLKRRNFAPETIQALKLAYRIIFRSSLLLK
                     TALEKVRTDVPDIPEVRQFIDFIQNTQRGICR"
     gene            233..1201
                     /locus_tag="PLR47_10690"
     CDS             233..1201
                     /locus_tag="PLR47_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG13461.1"
                     /translation="MNELRIGVVGIGHLGNYHLQKYALIPDCRITAVADVVAERAHRA
                     AAQYGCLAFQDHRDLLGRVDAVSIAVPTVSHYETAVTFLEAGVSALVEKPITVTLAEA
                     DALIAAAGRNNAVLQVGFIERFNPAVIALAKVIQQPLFIEAHRLHPFFERGTDVDVIL
                     DLMIHDLDVILHFVKSPVAAIDAVGVSVLSEMVDIANVRLKFASGCVANITASRVTGK
                     TLQKIRFFGIDGYHAVDYNKRDLVSLSKKISDSGRLDIFSNAVEVCQHDPLEAEIRSF
                     VAAVQNGARPVVSGEDGRNALDLALNIAREIKKAKDEILLSIQHGK"
     gene            1299..>1738
                     /locus_tag="PLR47_10695"
     CDS             1299..>1738
                     /locus_tag="PLR47_10695"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13462.1"
                     /translation="MAIYEKKVLIVAGEASGDLHGANLVRAMLESDPGLVFYGIGGPK
                     MEAAGVRCLARAGDMAVVGLTEVFSKLGMILGIMRKLKRSLWEEKPALIILIDYPDFN
                     MPLARAAKKRGVPVFYYISPQVWAWRRGRIKQLRQCVDRMAVILP"
BASE COUNT          373 a          497 c          492 g          376 t
ORIGIN      
        1 tggcctcggg aaacatggcc aaactctacg gcttgaatct catcggcctg aaaaggagaa
       61 atttcgctcc ggaaaccatc caggcgctga agctggccta ccgaattatc tttcgctctt
      121 ccctgctttt gaagacagcg ctggaaaagg tccggaccga cgttccggac attccggaag
      181 tacggcaatt catcgacttc attcagaaca ctcagcgagg gatctgccgg taatgaacga
      241 actccgcatc ggtgtggtcg gtatcggcca tctgggtaac tatcatctgc agaagtacgc
      301 cctgatcccg gactgccgca tcaccgccgt cgccgatgtg gtggcggagc gggcccaccg
      361 ggccgccgcc caatacggat gtctcgcctt tcaggatcac cgcgacctgc tgggtcgggt
      421 ggacgccgtg agcatcgccg tgcccacggt ttcccattac gaaacggccg ttacgtttct
      481 ggaagcgggc gtgtccgctc tcgtggaaaa gcccatcacc gtcactctcg ccgaagcgga
      541 cgctcttatc gccgccgccg gaaggaataa tgcggtcctg caggtagggt ttattgaaag
      601 gttcaatcct gccgtcatcg ccctggccaa agtcatccaa caacccctgt tcatcgaggc
      661 ccatcgcctc caccctttct tcgaacgggg tacggacgtg gacgtcatcc tcgatctcat
      721 gatccatgat ttagatgtca ttttgcattt tgtaaaatca cccgtagccg ccatcgatgc
      781 cgtaggggtg tcggtcctct ccgaaatggt ggatatcgcc aacgtccggc tcaaattcgc
      841 cagtggctgc gtagccaata tcaccgccag ccgcgtcacg ggtaagaccc ttcagaagat
      901 tcgtttcttc ggcatcgacg gttaccatgc cgtggactac aacaagcggg acctcgtttc
      961 tcttagcaaa aagattagtg attccggtag gctggatatt ttcagcaacg cggtggaagt
     1021 ctgccagcat gatccactcg aagcggaaat ccgttccttc gtcgccgccg tccaaaacgg
     1081 cgcccgccca gtcgtttccg gtgaggatgg acggaacgcc ctggacctgg cgctgaacat
     1141 cgcccgggaa attaagaagg ccaaggacga gatcctcctt tccatacagc atggaaaata
     1201 acaaaaaagc gggacacttc ccacatttct gattagcatg aaaatgcatc cggcgttctt
     1261 tcttgagtat tctcgtcgtt cgcgggtgtt gggtaaccat ggcaatttat gagaaaaagg
     1321 tcctgatcgt cgccggagag gcctcggggg atctccacgg cgccaatctg gtgcgggcca
     1381 tgctggagag tgatcccggt ctggttttct atggaatcgg cggcccgaag atggaagccg
     1441 ccggggtccg ttgcctggcc cgggccggag acatggccgt ggtgggactc accgaggttt
     1501 tttccaagct ggggatgatc ctgggcatca tgaggaagct gaagcggtcg ctgtgggaag
     1561 agaagccggc gctgattatt ctcatcgact atcccgattt caacatgccc ctggccagag
     1621 cggcaaaaaa acggggcgtc ccggtattct actacataag tccccaggta tgggcctggc
     1681 ggaggggccg gatcaagcaa ctgcgacaat gcgtggaccg gatggcggta atccttcc
//
LOCUS       DAOSIM010000341         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_59416_length_1737_cov_9.211653, whole genome shotgun sequence.
ACCESSION   DAOSIM010000341 DAOSIM010000000
VERSION     DAOSIM010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59416_length_1737_cov_9.211653"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            401..>1737
                     /locus_tag="PLR47_10700"
     CDS             401..>1737
                     /locus_tag="PLR47_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOG13463.1"
                     /translation="MNIAGSPQVPRPGMFATVRNRRGIVSAVEPFDGPEGRLHLVQLD
                     YKDDQLPVDEQLLWELESRKVLLEPTALPNITATDPMPAEDFDALLRAARWTAAMPFI
                     DPDGAGPLDRLPITSPFHGAVQVEDFQLVPLLKALQMPRVNLLIADDVGLGKTIEAGL
                     ILSELLLRRRIQRVLILTPASLRLQWRDEMWDKFSLPFDLVDRGESQNLRRRLGMDAN
                     PWRAFSRIIASYHYLRQEDVLAEFLAACRTPEGSPHLPWDLLIVDECHNLMPSPFGDE
                     SDLCRMLRLLAPQFEHRLFLSATPHNGHTRSFTGLLEILDPVRFSQTDELKKAEKERI
                     QQVVLRRLKREINARTNPPRFCRRNPPQALVLKLSEQEVALSVAFDGFRAAIRKLISS
                     GETRRRRAGCFAVEILGKRLLSCPTAFAESWRRCREGLAEENPAEETDVMAAKR"
BASE COUNT          401 a          482 c          436 g          418 t
ORIGIN      
        1 gaggagcgaa gcgacgtggc gatctcttca aattatcaaa tacttatgag attgcctcac
       61 ttcgtttgca atgacacaga gacattatgc agacagtttt gtaaaaagtc gtaatttcca
      121 ctttttgtca ttccgatgaa aatcggaatc ctgttgtttc aatatgttct ggatgccggt
      181 tttcaccggc atgacgattt tgacacattt tacgagaccg tcaattttct catttttgta
      241 acaattctat aggttgtgaa aacaataatc ctttcgtcga atgaaatatt tcttcgataa
      301 ctccttgaaa agtaaaggtg aatgatgtat gggtgccaac agcgaccgtt acttagcatt
      361 cccgtaaaaa acgaattaac gtgaaggaca tctcctaata atgaacattg caggcagtcc
      421 tcaggttccc cgccccggta tgtttgccac tgttcgcaac cgtcgcggta ttgtctctgc
      481 cgttgaacct ttcgatggcc cggaagggcg gttgcatctt gtccagcttg attacaaaga
      541 cgatcaactg cctgtggacg agcaactcct gtgggaactt gaatcaagga aggtgctcct
      601 tgaacccacg gctttgccca atataacggc aaccgatcca atgccggcgg aagatttcga
      661 cgcccttctt cgggctgcgc gatggacggc agcgatgcct tttatcgatc cggacggcgc
      721 cggaccgctg gaccgactac ccattaccag tccattccat ggcgccgttc aagtcgaaga
      781 cttccaactc gtcccgctcc tgaaagcttt gcagatgccg cgcgtgaatc ttttaattgc
      841 tgatgacgtc ggccttggca aaacgattga ggcaggactg attttgagcg aacttctcct
      901 ccggaggcgg atccagcgtg ttctgattct gacgcctgca tcgctgcgtc tccagtggcg
      961 agacgaaatg tgggacaagt tttccctgcc attcgatctt gtggacagag gggaaagcca
     1021 aaacctgcgc cggcgtcttg gcatggacgc caatccctgg cgggcattca gccgaatcat
     1081 cgcctcctat cactaccttc ggcaggaaga cgtcctggcc gaatttctgg ccgcctgccg
     1141 cacaccggag ggatcaccgc acctgccgtg ggacctgctt attgtcgatg aatgtcacaa
     1201 cctgatgcct tcacccttcg gggatgaaag cgacctttgc cgcatgcttc gcttattggc
     1261 gccccagttc gaacaccgcc tgtttttaag cgcgaccccc cacaatggtc atacccgcag
     1321 tttcaccggt cttcttgaaa tcctcgatcc cgtgcgtttc agccagacgg atgaattgaa
     1381 aaaagctgaa aaagaacgca tccagcaggt cgtcctgcgg cgtctcaaac gagaaatcaa
     1441 cgcacgcacg aatccaccaa ggttctgtcg gcgcaatcca ccccaggctc tggtcctgaa
     1501 actgtccgag caggaagtag ccctcagtgt tgcctttgac ggcttccgcg cggctatccg
     1561 aaaactgatc agcagcggtg aaacccgtcg ccgccgtgcc ggctgctttg cggtcgaaat
     1621 cctcggcaag cgccttctca gctgtccgac ggcttttgcg gaatcatggc ggcgctgtcg
     1681 cgaaggtctg gcggaggaaa atccggcgga agaaaccgac gtcatggcag ccaaacg
//
LOCUS       DAOSIM010000342         1714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_60606_length_1714_cov_9.174201, whole genome shotgun sequence.
ACCESSION   DAOSIM010000342 DAOSIM010000000
VERSION     DAOSIM010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60606_length_1714_cov_9.174201"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="PLR47_10705"
     CDS             complement(<1..124)
                     /locus_tag="PLR47_10705"
                     /inference="COORDINATES: protein motif:HMM:NF014077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOG13464.1"
                     /translation="MRILLTNDDGIYARGLAALYEELSKEADCLIVAPETEQSAV"
     gene            complement(266..1456)
                     /locus_tag="PLR47_10710"
     CDS             complement(266..1456)
                     /locus_tag="PLR47_10710"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HOG13465.1"
                     /translation="MNDSCLSAMSVFVLSLLLLGFTQQPMSAGASSESVKRHTVGCAF
                     PLSGRFAGEGKKAKDAVMLSARQFRARSPFSPGIVTADSGADPAMMKKAIAHLADGEN
                     VVAIIAVSGAAEAEAAAVEAQQRRVPVILITPKEGVTKAGDQVFQHFLTPEQQAEALA
                     DYAVNELRINVFGTLYPGDEYGEGMNRLFCADVHKNGGRVARSVAYPMAQTDFRQQIK
                     KLVGKKDKASRFPDARGEEEMPAELEGLFIPDSAFRARMITAQLAFYNVRKIRIFGTS
                     LWNTPDLLRKGNGYMEGAFFADSFYARGLAAETVNFADAYRAVYNREPQTMEALAYDT
                     MRLVLNILEDPEIETRDGFAQALLATKDIQGATGVISFGGGRVAQKEALILKVQDGEI
                     VQVR"
BASE COUNT          339 a          578 c          420 g          377 t
ORIGIN      
        1 ccacggcgga ctgttcggtt tccggcgcga caatcaggca gtccgcctct ttcgagagct
       61 cttcgtaaag agcggccaat ccccgcgcgt aaatcccgtc gtcattcgtc aataaaattc
      121 gcatctcatc actcgtcgct cgcagggagc ggtcgcgacc atccttacgg gtggcaagcc
      181 gttccctaca ttcagcctat cgcccattgc ctatgaccta ttacctcttt ttttctatcc
      241 accatccact attcactatc cgtccctacc ggacctgcac aatttccccg tcctgcacct
      301 tcaaaatcaa tgcctctttt tgcgccacgc ggccgccgcc gaaagaaatg acgcccgtgg
      361 cgccttgaat atccttcgtc gccagcagcg cctgcgcaaa cccgtcgcgg gtttcaattt
      421 cgggatcctc caaaatattc aggaccagtc tcatcgtgtc ataggccagc gcttccatcg
      481 tctgcggctc acggttgtaa acggcccggt aggcatccgc aaaattcacg gtttccgccg
      541 ccaaaccgcg ggcataaaag ctgtccgcga aaaaagcccc ttccatgtat ccgtttccct
      601 tgcgcagcag gtcgggcgta ttccacaaac tggtcccgaa aatccttatc ttcctgacat
      661 tgtaaaaagc aagctgcgcc gtgatcattc tcgcacgaaa agccgaatcc ggaataaaca
      721 ggccttcgag ttccgccggc atttcttctt cgccccgggc gtccgggaat ctcgatgcct
      781 tgtccttttt cccgacgagt tttttaatct gctgcctgaa gtcggtctgc gccatggggt
      841 acgcaaccga ccgcgcaacc ctgcccccgt ttttgtgaac gtcggcgcaa aaaagccggt
      901 tcatcccctc tccgtattca tccccgggat acagggtgcc gaacacgttg atccgcagct
      961 cgtttaccgc ataatccgcc agggcttccg cctgctgctc cggcgtcaga aaatgctgaa
     1021 acacctggtc gcccgccttc gtgacgccct ctttgggcgt gatcaaaatc accgggaccc
     1081 gccgttgctg ggcctccacg gcggcggctt ccgcctcggc ggcgccggaa acagcgatga
     1141 tcgccacgac attttccccg tcggccagat gggcgatcgc ttttttcatc atggccggat
     1201 cggcgcctga gtcggcggtg acgattcccg gggaaaacgg agaacgggcc ctgaactgcc
     1261 gcgcggacag catgacggca tctttcgcct ttttcccctc accggcaaaa cgtccgctca
     1321 gcggaaaagc gcaaccgacc gtatggcgtt tcacggattc ggaagacgcg cctgcgctca
     1381 tcggctgctg cgtaaatccc aaaagcagca gggacagaac aaaaacggac atggccgaca
     1441 gacaagaatc attcatgacg atttcccctt ctacggggat gctacaatat ttttgcggca
     1501 aaaaaaagcc cgaagccgcg tcacgcggct tcgggcccgt tttctcacga tttatttttt
     1561 cacttcttca aaatccgcgt ccacgacatc gtcgtccttc ttgccggcag acgcatcagc
     1621 gcccgcggct cccgcaccgc ccgccgcgcc cggtccgccg gcgccgcccg cggtcttggc
     1681 gtacatggct tccgccagtt tgtgcgacac gttc
//
LOCUS       DAOSIM010000343         1684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_62205_length_1684_cov_8.671578, whole genome shotgun sequence.
ACCESSION   DAOSIM010000343 DAOSIM010000000
VERSION     DAOSIM010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62205_length_1684_cov_8.671578"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1513
                     /locus_tag="PLR47_10715"
     CDS             <1..1513
                     /locus_tag="PLR47_10715"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOG13466.1"
                     /translation="GTDRSMQTANALAVVDGYVVTESEYYNEYSKLTDMIRAQYGAAL
                     TADMLKQMDLKKIAYDNLINRQIIIARAKDMKMQVSEDELRQMIMAMPALQTDGRFDN
                     YKYQQLLRFNRMTAEDFEAGQKITLAAGKIEAIIREGIKVSDREVLDLYTLQNQKINL
                     GFIAVAGADVAGKINPATPDLEEHLKKNSGAFRVAEQVKIQYLHFAGEAFAPAQITAS
                     DIRDHYNRHKDSYKGKDGKPLSPEQAAPIIAREMKQSRGLQDAYLEAKKAHDTIYQEQ
                     NFEDYAAKHQLKTQTVDFFPLNKPPQALASVKDLAQELAALERKDISKVMSDANGYFV
                     IRLDDKKAAYTPPLKAIEPEVRQAYLKSEQDRMAAQEAESLLAKLRKGESMEKLAGEK
                     GLRIQETGFFQPGGVIPKLGANPDAVNVLMSLSPSRPYPEKPMVIDHSHVIFRLKDVS
                     ALDMNDFEAKKEIYRKVAMNLKREEAMKTWLEGNKEALMKEKRLKIQKELKDL"
BASE COUNT          481 a          473 c          458 g          272 t
ORIGIN      
        1 cggcacggac cgaagcatgc agaccgccaa cgctctggcc gtcgttgacg gctatgtggt
       61 caccgaatcc gaatattaca atgaatactc caaactgacg gatatgatca gagcgcagta
      121 cggcgcggcg ctgacggcgg acatgctcaa gcagatggac ctgaaaaaaa tcgcctatga
      181 caatctgatc aaccgccaga tcatcatcgc cagggccaaa gacatgaaaa tgcaggtctc
      241 cgaagacgag cttcggcaga tgatcatggc catgcccgcc ctgcagacgg acggccggtt
      301 cgacaactac aaataccagc agcttttgcg cttcaaccgg atgaccgccg aagattttga
      361 ggccggacag aaaatcaccc tggcggccgg aaaaatagaa gccattatcc gcgaaggcat
      421 caaggtctcc gaccgggagg tgctggatct ctacaccctg cagaaccaga agatcaatct
      481 cggcttcatc gccgtcgccg gcgccgacgt cgcgggaaaa atcaatcccg cgaccccgga
      541 tctggaagag cacctcaaga aaaacagcgg cgcgtttcgc gttgccgaac aggtcaaaat
      601 tcaatacctg cactttgcgg gcgaggcctt cgcgccggcc caaatcaccg cctccgacat
      661 tcgggaccac tacaaccgcc acaaggacag ctacaagggc aaagacggaa agccactgtc
      721 gccggagcag gccgccccga tcattgcccg ggagatgaag cagagccgcg gactgcagga
      781 tgcctacctg gaagccaaaa aggcgcacga caccatttac caggagcaaa atttcgaaga
      841 ctacgcggca aaacaccaac tgaaaaccca gacggttgat tttttcccgc tcaacaagcc
      901 gccgcaggcg ctcgcttccg tcaaggatct ggcccaggag ctggccgccc tggagcgaaa
      961 ggacatcagc aaagtcatga gcgacgccaa cggctatttc gtgatccgtc tggatgacaa
     1021 aaaagccgcc tatacgccgc cgctcaaagc cattgaaccg gaagtccggc aagcctacct
     1081 gaaaagcgag caggacagaa tggccgccca ggaggccgaa agcctgcttg caaaactgcg
     1141 caaaggggaa agcatggaga aactggcggg ggaaaagggg ctgagaatcc aggaaaccgg
     1201 attcttccag cccggcggcg tcattcccaa actgggcgcg aatcccgacg ccgtgaatgt
     1261 gctgatgtcc ctgtccccga gccgccccta cccggaaaag cccatggtca tcgatcattc
     1321 ccatgtcatt ttcaggctca aagacgtaag tgcgctggac atgaacgatt tcgaagccaa
     1381 aaaggaaatc tacaggaaag tcgccatgaa cctgaagcgg gaagaagcga tgaaaacatg
     1441 gctggaggga aacaaagagg cgctgatgaa agaaaagcga ttgaaaattc aaaaagagct
     1501 gaaggattta taatcgatgc gcctctatgg cgccgaagcg gccgcgccca tgaaattttc
     1561 tttccgaccg gacgaaagaa aaacatgggt ggcaaagatg aaaaaaggga agaaccgcgg
     1621 ttcttccctt ttttatgttt ggtggagctg aggaggatcg aactcctgac ctcttgaatg
     1681 ccat
//
LOCUS       DAOSIM010000344         1667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_63158_length_1667_cov_10.510546, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000344 DAOSIM010000000
VERSION     DAOSIM010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63158_length_1667_cov_10.510546"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /gene="dapA"
                     /locus_tag="PLR47_10720"
     CDS             <1..601
                     /gene="dapA"
                     /locus_tag="PLR47_10720"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOG13467.1"
                     /translation="EAGADGALIACPYYNKPTQEGLYRHYQLIAKSVPLPIIPYNIPG
                     RTGVNMSPELMARLAEIDNIVGVKEASGSLKQMNDVLDLCGPEFDVLSGDDGFTLPLM
                     AIGGKGVISVASNIVPADMAAMVDAAEAGDFAAARALHAKMSPLFDVLFIEVNPIPVK
                     AALALMGRIVCEYRLPLCPMAPANHEKLKAALTRYGLIP"
     gene            654..1466
                     /gene="dapB"
                     /locus_tag="PLR47_10725"
     CDS             654..1466
                     /gene="dapB"
                     /locus_tag="PLR47_10725"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOG13468.1"
                     /translation="MVKVIVTGAGGKMGGRIIGMISAMENLRVVGAIEVKGHPIVGRD
                     LGHSLGTGRTGALVSDSLADCIDQADVVIDFTDHEASLNYLKIASEKNRAIVIGSTGF
                     TAEEMKTAQKLAEKTRCVLSPNMSVGVNVMLKVLDYCAGILYEDYDVEIIEAHHHLKK
                     DAPSGTALQMARVIAARLGRDLEKEAVYTRRGMIGERTRREIGIQTIRAGDIVGEHTV
                     IFGGIGERLEIVHRAQSRDNFAKGAIRAAQWIVHQKNGLYDMQDVLGLRDSR"
     gene            1477..>1667
                     /locus_tag="PLR47_10730"
     CDS             1477..>1667
                     /locus_tag="PLR47_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOG13469.1"
                     /translation="MLEGTLAYIGIGANLQDPLAQCRTAIERLSGAAGITLERISSFY
                     KTKPVIPESAGPEHRKELE"
BASE COUNT          385 a          452 c          523 g          307 t
ORIGIN      
        1 tgaggcgggc gccgacggcg cgctcatcgc ctgtccctac tacaacaagc cgacccagga
       61 agggctttac cggcattacc agctcatcgc gaagagcgtg cctttgccga tcattccgta
      121 caacattccc ggaaggaccg gcgtgaacat gtcgccggaa ctgatggccc gtctggctga
      181 aatcgacaac atcgtcggag tcaaggaggc ctccggatcc ctcaagcaga tgaacgacgt
      241 gctggacctg tgcggtccgg aattcgacgt tttgtccggc gacgatggat ttacgcttcc
      301 cctgatggcc atcggcggaa agggcgtcat ttccgtcgcg tccaatatcg ttcccgccga
      361 catggccgcg atggtcgacg ccgccgaagc aggggatttc gcggcggcgc gggcgctgca
      421 cgcgaaaatg agccctttgt ttgacgttct tttcatcgag gtgaatccga tccccgtgaa
      481 agccgctctg gcgctgatgg gcaggatcgt ctgcgaatac cggctgccgc tctgcccgat
      541 ggcgccggcg aatcatgaga agctcaaagc ggcattgacc cggtacggcc tgatcccgta
      601 ggacggcggg atgttttttc aagttcatgc ggaaacaaca aggagcaagc gttatggtaa
      661 aagtaattgt cacaggcgcc ggcggaaaaa tgggaggccg aatcatcggc atgatttcgg
      721 ctatggaaaa tcttcgcgtg gtaggggcca ttgaagtcaa gggccatccg attgtcggaa
      781 gagatctggg tcattccctg ggcacgggaa ggaccggcgc gctggtcagc gacagcctgg
      841 cggactgcat cgaccaggcg gatgtggtga tcgactttac ggatcatgaa gcgtcgctga
      901 actatctgaa aatcgccagc gaaaaaaacc gcgccatcgt aatcggctcc accgggttca
      961 ccgccgaaga aatgaaaacc gcgcaaaagc tggccgaaaa aacgcgctgc gttctttcgc
     1021 cgaacatgag cgtgggcgtc aatgtaatgc tgaaagtgct ggattactgc gcgggcatcc
     1081 tttatgagga ctacgacgtc gagatcatcg aagcgcacca ccatctgaaa aaagacgcgc
     1141 ccagcggaac ggcgctgcag atggcgcggg taatcgccgc caggctgggg cgggatctgg
     1201 aaaaagaagc ggtgtacacg cgccgcggca tgatcggcga acgcactcgc agagagatcg
     1261 gcattcagac cattcgggcc ggagacattg tgggcgaaca tacggtgatc ttcgggggaa
     1321 tcggggagag gctggaaatc gtccatcgcg cacagagccg ggataatttt gccaagggag
     1381 caatccgcgc ggcccagtgg atcgtccatc agaaaaacgg cctgtacgac atgcaggatg
     1441 ttttgggact gcgggattcc cgataggcgg gcggttatgc tggagggaac actggcctat
     1501 atcggaatcg gcgccaacct tcaagaccct ttggcgcagt gccgcacggc cattgaaaga
     1561 ctttccggcg ccgcgggaat tacgctggaa cggatttcct ccttttacaa aaccaagccg
     1621 gtgattccgg aatcggccgg accggaacac cgaaaggagc tggagaa
//
LOCUS       DAOSIM010000345         1662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_63426_length_1662_cov_7.711263, whole genome shotgun sequence.
ACCESSION   DAOSIM010000345 DAOSIM010000000
VERSION     DAOSIM010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63426_length_1662_cov_7.711263"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PLR47_10735"
     CDS             complement(<1..833)
                     /locus_tag="PLR47_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13470.1"
                     /translation="MGFRIQNNIAAMNAQRNLGISDTAMSKSLERLSSGYRINSAKDD
                     AAGLSISQGFRASIASYNVASRNVTEGNALLQVAEGAMDQVGNMLTRLKELATQAASA
                     NAGSNLDKINAEGNQLILEIDRIANSTEYAGSKLLNGTFGVSDTKTGTWETTNAGAGL
                     QSVSGMQSGVTYTVSAVTNADGDGTYNFTVSATLAGGQVAQTLYGVAGAAAGATAEVS
                     FASMGLKITLNDNFTATVGAGTIIASAAAASDFQVGAKNSVDDRIGVTLSSIKSSTLD
                     AG"
     gene            complement(839..1015)
                     /locus_tag="PLR47_10740"
     CDS             complement(839..1015)
                     /locus_tag="PLR47_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13471.1"
                     /translation="MLDREKFSGRQMQGGKDEKGGDQENAAGKGEISSHFLKEQQPSD
                     NWHGRHEIIKNQGG"
     gene            complement(1101..>1662)
                     /locus_tag="PLR47_10745"
     CDS             complement(1101..>1662)
                     /locus_tag="PLR47_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOG13472.1"
                     /translation="AGGQVAQTLYGVAGAAAGSTAEVSFASMGLKITLNENFTATAGA
                     GTIIASAAAASDFQVGSKNTIDDRIGVTLSSINSGSLNAGLVSDQMNTAAKAQSFLTT
                     VDSAISQLNTLRGGIGAAQNRFGYASANLATTIENFTAADSVIRDVDMASEMTSFTKN
                     QILLQAGTAMLSQANQAPQMVLSLFK"
BASE COUNT          290 a          475 c          513 g          384 t
ORIGIN      
        1 ccggcatcga gagtgctgga cttgatgctc gacagggtga cgccgatccg gtcatcgacc
       61 gagtttttcg cccccacctg gaagtccgag gcggcggcgg cgctggcgat gatcgtcccg
      121 gcgcctaccg tggcggtgaa gttgtcattg agggtgatct tcaggcccat ggaggcgaaa
      181 gaaacctccg cagtggcgcc cgccgcggcg cctgcgacgc cgtagagggt ctgcgcgacc
      241 tgaccgccgg ccagggtggc ggaaacagtg aagttatagg tgccgtcacc gtccgcattg
      301 gtcacggcgg ataccgtgta ggtcacgccg gactgcatcc ccgatacgga ctggaggccg
      361 gcccccgcat tagtggtttc ccaggtgcct gtcttggtgt cgctgacgcc gaaggtgccg
      421 ttcagaagct tggagccggc atactcggtg gagttggcga tccggtcgat ttccaggatc
      481 aactggttgc cttccgcgtt gatcttgtcc aggttgctgc ccgcattggc cgaggcggcc
      541 tgggtggcca gctccttgag acgggtcagc atgttgccca cctgatccat ggccccttcc
      601 gcgacctgga gcagggcgtt gccttccgtg acattcctgc tcgccacgtt gtagctggcg
      661 atagaagccc ggaatccctg agaaatggac agccccgccg catcatcctt ggcggaattg
      721 atccggtaac ccgatgagag acgctccagc gacttgctca tcgccgtgtc ggaaatgccc
      781 aggttccgct gtgcattcat ggccgctatg ttgttctgaa ttctgaatcc cataatcttt
      841 atcctccttg atttttgatt atttcatggc gtccgtgcca attgtcgctt ggttgttgtt
      901 cttttaaaaa gtgagaggaa atctcccctt tgcccgccgc gttttcctga tcaccccctt
      961 tctcatcctt tccgccctgc atctgccggc cggaaaattt ttccctgtcc agcactttga
     1021 tttcatatat agcaatgtac atgccaaaaa aaaccccgta gcagtcgcta cggggttttt
     1081 gaaagtttgt agatatatgt ttatttgaac aacgacagga ccatctgcgg ggcctgattg
     1141 gcctgggaga gcatggccgt acccgcctgg aggaggatct ggttcttcgt gaagctggtc
     1201 atttccgacg ccatatccac atcccggatg accgagtcgg cggcggtgaa gttttcgatc
     1261 gtcgttgcca ggttggcgga ggcgtaaccg aaacggttct gggctgcccc gatgccgccg
     1321 cgcagggtat tgagctggct gatggccgaa tccaccgtgg tgaggaatga ctgggccttg
     1381 gcggcggtgt tcatctgatc ggacaccagg ccggcattga gagagccgga attgatgctt
     1441 gacagggtga cgccgatccg gtcatctatg gtatttttcg atcccacctg gaagtccgag
     1501 gcggcggcgg cactggcgat gattgtcccg gcgcctgccg tggcggtgaa gttttcattg
     1561 agggtgatct tcaggcccat ggaggcgaaa gaaacctccg cggtggagcc cgccgcggcg
     1621 cctgcgacgc cgtagagggt ctgcgcgacc tgaccgccgg cc
//
LOCUS       DAOSIM010000346         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_64102_length_1649_cov_9.036386, whole genome shotgun sequence.
ACCESSION   DAOSIM010000346 DAOSIM010000000
VERSION     DAOSIM010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64102_length_1649_cov_9.036386"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..493)
                     /locus_tag="PLR47_10750"
     CDS             complement(293..493)
                     /locus_tag="PLR47_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOG13473.1"
                     /translation="MPEGKVKWFNEQKGFGFIEKDEGGDVFVHYSTIQGSGFKTLQEG
                     QRVSFDIQQGKKGPAAENVKPL"
     gene            947..>1649
                     /locus_tag="PLR47_10755"
     CDS             947..>1649
                     /locus_tag="PLR47_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13474.1"
                     /translation="MMKRLKICLINPRFEPSYWGMDYALPIYPGSKLCTMTTGALPHL
                     AGLIPDHDVYLLDENVEEIDFGTMKDYDLVGVTGMIVQKDRMRSILLRLREIKVFTAV
                     GGAYASVEEDFFTGLCDVLFSGEADDTWPEFVAAFAAGSPCKKLYRQENRTVMTTLNK
                     PRYDLLKVDRYASGSVQFSRGCPFQCEFCDIIVTFGRRPRTKSPEQLLEELDDMRKVG
                     FFSCFIVDDNFIGDKK"
BASE COUNT          370 a          432 c          425 g          422 t
ORIGIN      
        1 ccgcaggccg ccctgaatgc ggaaacggcc gcggcgatcc ttctgaggga tttttctccg
       61 gcaagaataa tcgagaccca tctgcggaat gtggaagaac cgcgggccgg tgcggatcca
      121 ccctgacaca catcggcaaa gctgggtatc gttcataatt tccgccgggt ccgaccgcta
      181 cgaacctttt ccggtttctg ccagaaattc tttccggatc gggaagcaag aagatcaaaa
      241 aaagacgccg cggtcgatga aaccgcggcg tcttgatcgg ttacgctgag ggttatagag
      301 gctttacgtt ttccgctgcc ggacccttct tgccctgctg gatgtcaaag cttacgcgct
      361 gaccttcctg aagggtttta aaaccggagc cctggatggt gctgtagtga acaaaaacat
      421 cgccgccttc atctttttcg atgaagccga agcctttttg ctcattgaac catttcactt
      481 ttccttctgg cattttctgc atcctccttt ccgtaaagtc ggattgcatc tctgacagaa
      541 agataagctc cgctgtactt caaaatgcct ttttccaatc tttactactt gttctccccc
      601 ccgcttgtca cagcggcgga gataagatct atgggtcggc atatagtacg gcgcctgagc
      661 gctgtcaagc attttgtcaa aatattttca tgcccaagat tcggtgaaga tgccgaagat
      721 atttacatcg tttgtggcgc agccatggag aagaaaggtt ttgtaaaacg ttaatcaata
      781 attcttgact ttgttgggaa acagacatat taaaagttgg atgtgtctgc cgccataggg
      841 cggcgccggc ccgaaagcat aagacgaggc ctcacaaaag agattttgaa gtgtcatgga
      901 cagccgccat gttctttgag atggcggctt tttttgtacc tcctgaatga tgaaacgact
      961 gaagatttgc ctgatcaatc cccggttcga gccttcctat tgggggatgg actacgccct
     1021 gcccatttac cccggcagca agctgtgcac tatgaccacc ggcgccttgc cccatctggc
     1081 cgggctgatc ccggatcacg acgtctatct tctcgatgaa aatgtcgagg aaatcgattt
     1141 cgggaccatg aaggattacg atctggtggg cgttacgggc atgatcgtcc agaaggaccg
     1201 gatgcgctcc attctcctcc gtctccggga aataaaggtt ttcaccgccg taggcggcgc
     1261 ctatgcctct gtggaggagg atttcttcac cggcctgtgc gatgtgcttt tttccggcga
     1321 ggccgatgat acctggcccg agtttgtggc cgccttcgct gccggcagtc cgtgtaaaaa
     1381 actctaccgc caggaaaatc gaacagtgat gaccaccctg aataaaccgc gctacgacct
     1441 cctgaaggtt gaccggtacg cgtcgggatc ggttcagttt tcacgcggtt gtccttttca
     1501 atgcgagttt tgcgacatta ttgttacctt tggacgccgc ccgcggacca agagtccgga
     1561 acagcttctc gaagagctgg acgatatgcg caaagtcgga tttttctcct gctttatcgt
     1621 ggacgacaat ttcatcggcg acaaaaagg
//
LOCUS       DAOSIM010000347         1641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_64509_length_1641_cov_7.382093, whole genome shotgun sequence.
ACCESSION   DAOSIM010000347 DAOSIM010000000
VERSION     DAOSIM010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64509_length_1641_cov_7.382093"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /gene="ccsA"
                     /locus_tag="PLR47_10760"
     CDS             complement(<1..423)
                     /gene="ccsA"
                     /locus_tag="PLR47_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOG13475.1"
                     /translation="MIHTTILSWVTFLYFAAFVFYLFRMILGREFWGKAATGAALAGL
                     AAQSVALALRWRASYDLGFGHAPLSNFYESLIFFAWTIVLLYLLMERRLKNRSIGVFV
                     LPVAFLIMAYASIAPGMNQRIEPLIPALQSNWLTSHVLT"
     gene            complement(420..>1641)
                     /locus_tag="PLR47_10765"
     CDS             complement(420..>1641)
                     /locus_tag="PLR47_10765"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOG13476.1"
                     /translation="KMQIFDLYHSVWFILLSGLLAVNLIICSLDRFPLAWRRFRMKHE
                     PENEDVFRDVPEENTFLANADVQAAADEALSLLRRKYRAVERADGEQGVWLCAQKGRF
                     SLFGVYIVHVSILVLIAGAILGSVFGVEGYVHIPEGETAEAISLRSGGQPLPLPFAVR
                     CDKFTVEFYESGMPKTFQSDLTFLKDGAAVRTAKLLVNHPVEFGGFRFYQASYGAAPG
                     GKATLALAQDGGRRDVMNVAQGFVFDLPGGEGTFQVLRVEENLMKMGPAVKVAVTSRR
                     GEEAVFWVFQQIEPIKKMSPDIFEQVPVFNPALFRPYTFSLLGLEEKYYTGLQVNRDP
                     GTPVVAVSALLLIGGLMLILFSYARAVWIRVAPSENHVLVAIAGRSYKNQPGLQKEMQ
                     YLLAELKDHLEKRQ"
BASE COUNT          398 a          505 c          448 g          290 t
ORIGIN      
        1 ggtcagcacg tgcgaggtca gccagttgct ctgcagggcg ggaatcagcg gctcgatgcg
       61 ctgattcatt cccggagcga tggacgcgta ggccatgatc agaaacgcaa cgggcagaac
      121 gaaaacgccg atgctccggt tcttcagacg ccgctccatg agcaggtaca gaagaacgat
      181 cgtccaggcg aaaaaaatca gagattcgta aaaattggac aggggcgcat gaccgaaacc
      241 cagatcgtaa gacgccctcc agcggagggc aagcgcaacg ctctgggcgg ccaaaccggc
      301 cagcgccgct cccgtggccg cctttcccca gaactcgcgc cccagaatca tccggaaaag
      361 ataaaaaaca aaagcggcaa aatagaggaa ggtcacccag cttaaaatcg tcgtatggat
      421 cattggcgct tctccagatg gtccttcagc tccgcgagca ggtattgcat ttctttctgc
      481 aggcccggct gattcttgta gctccggccg gcaatcgcga ccagcacatg attctccgac
      541 ggcgcgacac ggatccaaac cgcgcgggcg taggaaaaca gaatcagcat caggcccccg
      601 atcaacagca aggcggaaac cgccaccacc ggcgttccgg ggtcgcggtt gacctgcaag
      661 ccggtgtaat atttttcctc gaggcccaaa agcgaaaagg tgtagggacg aaacagagcg
      721 ggattgaata ccggcacctg ctcgaaaatg tccgggctca tttttttaat gggttcgatc
      781 tgctggaaca cccagaaaac agcctcttcc cccctgcggg aggtgacggc taccttgacg
      841 gcggggccca tcttcatcag attttcctca acgcgcagca cctgaaacgt tccctccccg
      901 cccggcaggt cgaagacgaa cccctgcgcg acattcatca cgtcgcggcg gccgccgtcc
      961 tgcgccagcg ccagcgtcgc cttgcctccc ggcgccgcgc cgtagctcgc ctgataaaaa
     1021 cggaaccccc caaattcaac gggatgattg accagaagct tcgcggttcg gaccgccgcg
     1081 ccgtccttga gaaaggtcag gtcggactga aacgttttgg gcatgccgct ttcgtaaaac
     1141 tccacggtga atttgtcgca gcgcacggca aagggaagcg gcaacggctg accgccgctg
     1201 cgcagggaaa tcgcctccgc cgtttcccct tccggaatgt ggacatatcc ttcaacgccg
     1261 aaaaccgatc caaggatcgc gcccgctatc aaaaccagga tgctcacatg gacgatgtaa
     1321 acaccgaaaa gagaaaagcg tcccttctgc gcgcaaagcc ataccccttg ttccccgtca
     1381 gccctttcca cggcgcgata ttttcttcgc agcagggaca gggcttcgtc cgccgccgcc
     1441 tgaacgtccg cgttcgcgag aaacgtattt tcttccggca catcccgaaa gacgtcttca
     1501 ttttcaggct catgcttcat tcggaagcgg cgccacgcca ggggaaagcg atccagcgaa
     1561 cagatgatga ggttgacggc cagaagaccc gataaaagga tgaaccacac ggagtgatac
     1621 agatcgaaga tctgcatttt c
//
LOCUS       DAOSIM010000348         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_65123_length_1630_cov_8.699048, whole genome shotgun sequence.
ACCESSION   DAOSIM010000348 DAOSIM010000000
VERSION     DAOSIM010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65123_length_1630_cov_8.699048"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..439
                     /gene="dut"
                     /locus_tag="PLR47_10770"
     CDS             5..439
                     /gene="dut"
                     /locus_tag="PLR47_10770"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOG13477.1"
                     /translation="MNVQIRKIAGNEDLPLPEYMTRAAAGMDLFAAVAAEETIAPGGR
                     KKIPTGIAVALPEGYEAQIRPRSGLALHQGVTLLNAPGTIDADYRGEIALIVVNHGEA
                     PFVVKRGARLAQMVVQKICRVQWVETVRLDETQRGAGGFGHT"
     gene            515..>1630
                     /locus_tag="PLR47_10775"
     CDS             515..>1630
                     /locus_tag="PLR47_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOG13478.1"
                     /translation="MKKFKVQKTFQEINEKIKKGRAVVVTAEEMIDVVERHGDVEAAR
                     RVDVVTTGTFGAMCSSGAFLNFGHTAPRIRASRVWLNDVPAYGGIAAVDCYLGATEVV
                     EGDPQNSVYPGEFNYGGGHVIEDLVSGNVVKLRAEGYGTDCYPSRKFEKNITISDLRD
                     AFLFNPRNAYQNYNCAVNLSDTTIYTYMGILRPRMANVSYSTSGQLSPLFNDPYYRTI
                     GIGTRIFLGGAQGFVAWPGTQHNPNAMRSANGVPKEGAGTLAVIGNLKEMSPEWLTGA
                     SILGYGVSLFVGIGIPIPILDEDMARFTAVKDEDICAQVYDYSMDYPKGNPKPVAEVN
                     YRDLRSGTVMIQDKKVIAAPLSSYYKAHQIAELLKGWI"
BASE COUNT          357 a          475 c          492 g          306 t
ORIGIN      
        1 aaccatcaac gttcagattc ggaaaattgc cggcaacgag gatcttcccc tgcccgaata
       61 catgacccgt gcggcggcgg gcatggacct ctttgcggcg gtcgcggcgg aggaaaccat
      121 tgcgcccggc gggcgcaaaa aaattccgac cggcatcgcc gtggccctgc cggaaggcta
      181 tgaggcgcag attcgcccgc gcagcggcct ggcgcttcat cagggcgtca cgcttttgaa
      241 cgctccggga accatcgatg cggactaccg cggcgaaatc gccctgattg tcgtcaatca
      301 cggagaagct cccttcgtgg tgaaaagagg agcgaggctg gcgcagatgg tggttcagaa
      361 aatctgccgg gttcagtggg tggaaaccgt ccggctggac gagacgcaaa gaggcgcggg
      421 cggtttcggg catacctgag cggcggccgg gattttgcaa ttatcggcaa atcgtggtaa
      481 gaaagcgtca aaatcggccg tccacaaggt gcgcatgaaa aaattcaaag ttcaaaaaac
      541 ctttcaggaa atcaacgaaa agatcaaaaa ggggcgcgcc gttgtcgtca ccgccgagga
      601 aatgatcgac gtggtggagc gccacggcga tgtcgaggcg gcccgccgcg tcgatgtcgt
      661 cacgaccggc accttcggcg cgatgtgctc ttcgggcgcc tttctgaatt tcgggcatac
      721 cgctccgcgc atccgcgcgt cccgcgtctg gttaaacgac gtgccggcct atggcgggat
      781 tgccgcggtc gactgctatc tgggcgcgac ggaagtcgtc gagggagatc cgcaaaacag
      841 cgtctatccc ggcgaattca attacggcgg cggccatgtg atcgaggacc tggtgtccgg
      901 caacgtggtg aaattacggg ctgaggggta cgggacggat tgctatccgt cgcgcaaatt
      961 tgaaaaaaac atcaccatca gcgatctgcg cgacgccttc ctgttcaatc cgcgcaacgc
     1021 ctatcagaac tacaactgcg ccgtgaacct ttccgacacg acgatttaca cctacatggg
     1081 catactgcgt ccgcgcatgg ccaacgtttc ctattcgaca tccggccagc tcagccccct
     1141 gttcaacgat ccctattacc ggacgatcgg catcggcacg cggattttcc tgggcggcgc
     1201 ccagggcttt gtcgcctggc ccggaacgca gcataatccc aacgcgatgc gttccgcaaa
     1261 cggcgtgccc aaagaagggg cgggcactct ggcggtgatc ggcaatctga aggagatgag
     1321 ccccgagtgg ctgaccggcg ccagcatttt aggctacggc gtttccctgt ttgtcggcat
     1381 cggcattccc attcccattc tggatgaaga catggcgcgc ttcaccgcgg tgaaagacga
     1441 ggatatctgc gcccaggtgt atgattacag catggactat cccaagggca atcccaagcc
     1501 cgtcgcggag gtcaactacc gcgacctgcg cagcggaacg gtgatgattc aggataaaaa
     1561 agtcattgcc gccccgctgt ccagttacta caaggcccat cagattgccg aacttctgaa
     1621 gggctggatc
//
LOCUS       DAOSIM010000349         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_65125_length_1630_cov_7.161270, whole genome shotgun sequence.
ACCESSION   DAOSIM010000349 DAOSIM010000000
VERSION     DAOSIM010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65125_length_1630_cov_7.161270"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PLR47_10780"
     CDS             <1..645
                     /locus_tag="PLR47_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG13479.1"
                     /translation="VHVGIIIRNDRLTPILEKVREAKPDILVSTGDLLDGELDNIMTD
                     AERFAAIPAPVGKFAVLGNHEYYAGVSRSIEFTEAAGFQLLRDEVAQASGITIFGEDD
                     ITGRKPGSTQKSALFLKSLAEKQNGFVLLLKHQPHIRETANFNLQLSGHTHGGQLFPF
                     GFIVKLYFPRIYGLHTLAENKLLYISRGTGTWGPPVRIFAPPEITVIDLIGKKN"
     gene            686..1498
                     /locus_tag="PLR47_10785"
     CDS             686..1498
                     /locus_tag="PLR47_10785"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG13480.1"
                     /translation="MKKNYIHGYHPRENIRLQDQASTLAELLHSDTAYPAGSRVLEAG
                     CGVGAQTVALARSSPGASITAIDLSESSLAEAGRKAAAAGLTNVQFRQADIFHLPFAA
                     EAFDHVFVCFVLEHLTHPLEALGALQSVLRKGGTMTVIEGDHGSAYFHPASESAHRAI
                     QCQVELQRRAGGNALIGRELYPLLCRAGFSKVRVSPRMVYVDSSRRVLADGFTRKTFT
                     AMIEGVRQSALEAGLIEEETFDRGIRDLYRTAEDDGVFCYTFFKAVGEKPLK"
BASE COUNT          353 a          462 c          459 g          356 t
ORIGIN      
        1 gtccatgtcg gcatcattat ccgcaacgac cgcctcacgc ccattctgga gaaggtccgg
       61 gaggccaaac ccgacattct ggtttctacc ggcgacctgc tcgacggcga actggacaac
      121 atcatgacgg atgccgaacg ctttgccgca atcccggcgc ctgtcgggaa atttgccgtt
      181 ctgggcaatc atgaatatta tgcgggcgtt tcgcgctcca ttgaatttac cgaagccgcc
      241 ggctttcaac tgttgcgtga cgaggtggcg caggcgtctg ggattacgat tttcggcgaa
      301 gacgacatca ccggccggaa gcccggctct acgcaaaaaa gcgcgctgtt tctgaaatcg
      361 ctggccgaaa agcaaaacgg ctttgttctg cttttgaagc atcagccgca cattcgggaa
      421 acggcaaatt tcaacttgca gctttccggc cacacgcacg gcggccagct ttttcccttc
      481 ggcttcatcg tcaaacttta tttcccaaga atatacggtt tgcataccct ggcggaaaac
      541 aagctgctgt atatcagccg gggcacgggc acctgggggc cgcccgttcg cattttcgcc
      601 ccgccggaaa tcaccgtgat cgatctgatc ggtaaaaaga actaattccg tccaaaaaac
      661 cgatttcaaa gcaggcaggg tccggatgaa gaaaaactat atccacggtt atcacccgcg
      721 cgaaaatatc cggttacagg accaggcctc cacgctggcc gaactgctgc attccgacac
      781 ggcgtacccc gcgggaagcc gcgttcttga agcgggctgc ggcgtcgggg cgcaaaccgt
      841 tgctctggcc cgcagcagtc cgggcgcctc gattaccgcc atcgacctgt cggagagctc
      901 acttgccgag gccggccgga aagccgcggc ggcaggcctg acaaacgttc aattccggca
      961 ggccgatatt tttcatctgc ccttcgcggc agaggccttc gaccacgttt ttgtctgttt
     1021 tgtcctggag catctgacac atccgttgga ggccctgggc gccctgcaaa gcgtcctccg
     1081 gaaaggcggc acgatgacgg ttattgaagg ggatcacgga tccgcctatt tccacccggc
     1141 aagcgaatcc gcccaccggg ccattcagtg tcaggttgag cttcagcgca gggccggggg
     1201 caacgccctg atcgggcggg agctttatcc cctgctgtgc cgggctggtt tcagcaaggt
     1261 tcgcgtctcc ccccgaatgg tgtacgtcga ttcaagcaga cgcgtactgg ccgacggttt
     1321 caccaggaaa accttcaccg ccatgattga aggcgttcgc caatccgctc tggaagcggg
     1381 cctgattgaa gaagaaacgt tcgatcgggg cattcgggac ctttacagaa cggctgagga
     1441 tgacggcgtg ttttgttata cgttctttaa ggctgtcggc gaaaagccat tgaaatagag
     1501 ttcttttgaa ataaagcgga aaaaaacacg gcatcgccgt gggcgatgcc atgtgcctgc
     1561 gaaaattatt gaagagcgtt tacttttttg gttaagcggg aaattttccg ggaggcgttt
     1621 ttcttgtgaa
//
LOCUS       DAOSIM010000350         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_65545_length_1623_cov_7.707270, whole genome shotgun sequence.
ACCESSION   DAOSIM010000350 DAOSIM010000000
VERSION     DAOSIM010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65545_length_1623_cov_7.707270"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..609)
                     /locus_tag="PLR47_10790"
     CDS             complement(178..609)
                     /locus_tag="PLR47_10790"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOG13481.1"
                     /translation="MKVRPVKLFSDGACRGNPGPGGGGAVITDAEGRVLWEGREYFGF
                     CTNNIAEYKALILGLKGALACGCKNLEIYLDSELLANQINGSYKVKNEKLKQLMQDVR
                     KLLSSFESAMVRHVPRCRNATADRLANMAIDEHADRLKAEG"
     gene            complement(616..1335)
                     /locus_tag="PLR47_10795"
     CDS             complement(616..1335)
                     /locus_tag="PLR47_10795"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOG13482.1"
                     /translation="MKEELRLLIELQECDSQLVALAAKKTRLPEKILAMENEFQACRE
                     KLSLNRQKHDELKAKRAEYEAAIKKLGDGMTKTKERLLEVKNNKEYQAMLKEIETAEK
                     TSGEMETRMIALLEDTDKLFVLMREDDEALQRETRRYEQDKKAIEEDLNAVDADAESW
                     ARKRAELQKQISADLQARYEKVKKRNNGLGVISVWKAVCSGCHMNIPPQLYNELQRSN
                     EMLSCPNCNRIMYFKNQEKSE"
BASE COUNT          309 a          464 c          372 g          478 t
ORIGIN      
        1 gtcgccgtta gcgaccatcc tgccctgtgg agctcggact ttcctccggc agagcagact
       61 ctgcccgcga tcatccgatt ttctccggcc tggaacctat cctacaattg attcggattt
      121 tgttcaagac aacttctaaa aagatattta gcgccttttt ccttcagttt caaaccttca
      181 gccttcagcc ttcagtctat cagcatgttc atcaatggcc atgttggcca gccggtcggc
      241 tgtcgcattg cggcaccgcg gaacatgccg gaccattgcg gattcaaaag aagacagcag
      301 tttccggaca tcctgcatca attgtttcag cttttcgttt ttgactttgt aggagccgtt
      361 gatctggttg gccaaaagct ccgaatccag ataaatttcc aggtttttgc atccgcaggc
      421 caatgccccc ttcaggccaa ggatcaatgc tttatattcc gcgatattat tggtgcagaa
      481 accgaagtac tccctgccct cccacagaac ccggccttcg gcgtcggtaa tcaccgcccc
      541 gcctcccccc gggccgggat ttccccggca ggcgccgtcg ctgaataatt tgaccggccg
      601 cactttcatc aaaggctact cagacttttc ctgattttta aaatacataa tccggttgca
      661 gttggggcag ctcagcattt cattggaacg ctgcagttca ttgtagagct gcggcggaat
      721 gttcatgtga cagccgctgc aaaccgcttt ccaaacggaa atcacgccca gcccgttgtt
      781 gcgctttttt actttttcgt acctcgcctg aagatccgcg gagatttgtt tctgcagttc
      841 ggcgcgcttg cgggcccagc tttccgcatc ggcgtccacg gcattcaaat cctcctcgat
      901 ggctttcttg tcctgttcgt accggcgggt ttcccgctgc agggcttcat catcctcgcg
      961 catcagcacg aaaagcttat ccgtgtcttc cagaagggcg atcatccttg tctccatctc
     1021 accgcttgtt ttctccgcgg tctcgatctc cttgagcatg gcctgatatt ctttattgtt
     1081 ttttacttcc aggagtcttt ctttggtttt ggtcattcca tcgccgagct ttttaatggc
     1141 ggcttcgtat tccgcgcgtt tggccttgag ttcatcgtgc ttttgccggt ttagcgaaag
     1201 cttctcccga caggcctgaa attcattttc catcgcaagg attttctccg gcagtctggt
     1261 ctttttagcg gcaagcgcga ccagctgcga atcgcactcc tgcaactcaa tcaacagccg
     1321 taattcttct ttcactcctg ttctccttct ttataatttt tcgtcccccg gggggcgcaa
     1381 aaaaaatgca ccttttgagg ggtgcatttt cagatcgtcg gtcgatgggt ccggttttgg
     1441 gcgccgcgat agaataatcg aaggtttctg atcgcggctt ttgcttcgac agttttgtcg
     1501 cagcagctcc ttgcttgtaa aaatcgcatt tttccggcca tcgtcaatat tttatccata
     1561 aaatctggtg ggcccacctg gaatcgaacc agggacctac cggttatgag ccggtggctc
     1621 tac
//
LOCUS       DAOSIM010000351         1591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_67428_length_1591_cov_6.602214, whole genome shotgun sequence.
ACCESSION   DAOSIM010000351 DAOSIM010000000
VERSION     DAOSIM010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67428_length_1591_cov_6.602214"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /gene="prs"
                     /locus_tag="PLR47_10800"
     CDS             <1..544
                     /gene="prs"
                     /locus_tag="PLR47_10800"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOG13483.1"
                     /translation="QIQGFFNIPVDNLFATPVLIDYMKKKYTDDTVVVSPDTGGVERA
                     RAFGKRLGASLAIIDKRREGPNEAQVMNIIGNVEGKRVIILDDMVDTAGTVVQAAKAL
                     AEAGALEVSVCCTHPVLSGPAIDRIEKSAIREFIVTDTIPLCERAAGCERIKVLSVSG
                     LLSEAVRRIYYNDSVSSLFI"
     gene            559..1227
                     /locus_tag="PLR47_10805"
     CDS             559..1227
                     /locus_tag="PLR47_10805"
                     /inference="COORDINATES: protein motif:HMM:NF004139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOG13484.1"
                     /translation="MEITDLAAQVRKEQKKGPARRLRQQGLVPAIFYGKATENIQLAV
                     KSADLFRLHKEKKDHAFIKLIIDDGGSKVEKLSLIKELQTQPLSGKFYHADFYEVDMK
                     KKLAFDVELNFTGRAIGVENGGELQHIKRDIRVLCLPTDLPDHIDVDISAIDIGHSLK
                     VKDIVIPEGLAHLDPPEAAVVAVAAIRVVKAEEAQEAPAEGAEPAEGAAAAEPAKEDK
                     KEKK"
     gene            1312..>1591
                     /gene="pth"
                     /locus_tag="PLR47_10810"
     CDS             1312..>1591
                     /gene="pth"
                     /locus_tag="PLR47_10810"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOG13485.1"
                     /translation="MVYLVIGLGNPGKRYESTRHNIGFMVVEKLAERWKIELKHKSFN
                     ALWGKGAMDKKQVLLAKPQTFMNLSGTAARRLQSFFKTEISNLIVIHDD"
BASE COUNT          414 a          384 c          476 g          317 t
ORIGIN      
        1 ccagattcag ggcttcttca acattcccgt ggacaacctc tttgccacgc cggttctgat
       61 agactacatg aagaaaaaat acacggatga tacggtagtg gtttctccgg ataccggagg
      121 cgtcgagcgg gcccgggcgt tcggcaaaag gctgggcgcc tctctggcga tcatcgacaa
      181 acgaagggaa ggccccaatg aagcccaggt gatgaacatc atcggcaacg tcgaggggaa
      241 aagggtcatt atcctggacg atatggtcga tacggcgggg accgtcgtgc aggcggccaa
      301 ggcgctggcg gaagcgggcg ctctggaagt gtctgtctgc tgcacgcacc cggttttgtc
      361 cggtccggcc atcgatcgca tcgagaaatc cgcgatcagg gagtttattg tgacggatac
      421 gatccctctt tgcgaaaggg ccgccggctg cgaaagaatc aaggttttgt cggtgtccgg
      481 tctgttgtcc gaagcggtgc gccgtattta ttacaacgat tcagtaagtt cattatttat
      541 ttaggaggga aggggtaaat ggaaataacc gatttagcgg cccaggtccg taaagaacag
      601 aagaagggcc ctgcccgaag gctgcggcag cagggacttg tgcccgccat tttttacggc
      661 aaagccacgg aaaacatcca gttggccgtg aaaagcgccg acctgtttcg gctgcataag
      721 gaaaagaaag accacgcttt catcaagctg atcatcgacg acggcggcag caaagtcgaa
      781 aagctttccc tcatcaagga actgcagacc cagcccctga gcggaaaatt ctaccacgcg
      841 gatttttatg aagtggacat gaaaaagaaa ctggcctttg acgtggagct gaactttacc
      901 ggcagggcga tcggcgtgga aaacggcggg gaactccagc acatcaaaag agacatcagg
      961 gtgctgtgcc tgccgacgga tctgcccgac cacatcgatg ttgatatttc ggccatcgac
     1021 atcggacatt cgctgaaagt gaaagacatc gtcattccag agggtttggc ccatctggat
     1081 cctccggaag ccgccgtcgt ggcagtcgcc gccattcggg tggtcaaggc tgaggaagcg
     1141 caggaagcgc cggcggaagg cgctgaaccc gcggaaggcg cagcagccgc tgaaccggca
     1201 aaagaggaca agaaggaaaa gaagtaaggc gacaggtgca ttatacggga gatcatgcgt
     1261 ctcttcagct ttggaaaata tctgttgggt tttcataaag agaggggaca aatggtttat
     1321 ctggttatcg gactgggcaa tccgggaaag cgctatgaat ccacccggca caatatcggc
     1381 ttcatggtgg tcgagaagct ggccgaaagg tggaaaatag agctgaaaca taagagtttc
     1441 aatgccctgt ggggcaaagg ggccatggac aaaaaacagg tcctgctggc caagccccag
     1501 acgttcatga atttgagtgg tactgccgca aggcggctgc agtcgttctt taaaacggaa
     1561 atcagcaatc tcatcgttat tcacgatgat c
//
LOCUS       DAOSIM010000352         1588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_67597_length_1588_cov_7.614481, whole genome shotgun sequence.
ACCESSION   DAOSIM010000352 DAOSIM010000000
VERSION     DAOSIM010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67597_length_1588_cov_7.614481"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PLR47_10815"
     CDS             complement(<1..285)
                     /locus_tag="PLR47_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13486.1"
                     /translation="MRNYADVPNLHTRIHAMRSRLFSLRDYVLMIREPETLPGHIFGI
                     QDSTEAKETLFEEQIAPVFNLATAHEQYTPFFIAHLRQYEAHNAKILLAMA"
     gene            complement(292..600)
                     /locus_tag="PLR47_10820"
     CDS             complement(292..600)
                     /locus_tag="PLR47_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13487.1"
                     /translation="MDEVITRIVAVERQCSADVEQARQECAKNIESRKQSLEDQKTRA
                     FAQIIAAEKLRLTKAMEEAKRQTEETSLAFQRDCEVPFQNQVLAQTIQEEIISILFEG
                     "
     gene            618..896
                     /locus_tag="PLR47_10825"
     CDS             618..896
                     /locus_tag="PLR47_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13488.1"
                     /translation="MPAEWLTFVFSSAWIDPDEKRTAGLCAREICVLAVFGEIITVQI
                     LHVNENIDKNKDRDALILLHNAVVVPVQRIDLPQTARQGKRNNMFTGD"
     gene            897..1226
                     /locus_tag="PLR47_10830"
     CDS             897..1226
                     /locus_tag="PLR47_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13489.1"
                     /translation="MDEYLHVPRGGEISFMAVTLKVTAEGCLDCGNRQLLYVEGLTTV
                     GSVCCGAVECRVIYVPGFIVSRHCKTDEASGHPVSRVEPVTNPAALDEVKKLLVKTFP
                     SSLFLFV"
     gene            complement(1234..>1588)
                     /locus_tag="PLR47_10835"
     CDS             complement(1234..>1588)
                     /locus_tag="PLR47_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13490.1"
                     /translation="IGLLTGCYCAASLYIPALKPAGLTSGPEAWWLAMLVAGRTTQGF
                     VIGLADGIDLHPALRGAGIGALMTLQLAMMPLFGPFKLGAVLLMAAGVIYGIIEDVAA
                     TWVARRFPASRQGAA"
BASE COUNT          347 a          434 c          433 g          374 t
ORIGIN      
        1 cgccatcgcc agaagaatct tggcgttgtg cgcctcatac tggcggagat gggcaataaa
       61 aaacggcgta tattgttcat gggccgtggc cagattaaat accggcgcaa tctgctcctc
      121 gaagagcgtc tccttggctt ccgtcgaatc ctggattccg aagatatgac cgggcagcgt
      181 ctccggctcc cgaatcatca ggacatagtc ccgaagggag aagaggcggc ttctcatggc
      241 gtgaatccgc gtatgcaaat tgggcacatc cgcataattt cgcatctttt tctacccttc
      301 gaacaagatg gaaatgatct cttcctgaat ggtctgcgcc agaacctggt tttgaaaagg
      361 tacttcgcag tctctttgaa aagcgagcga agtctcctcg gtttgccttt tggcttcctc
      421 cattgctttt gtcagacgaa gcttctccgc ggcaatgatt tgcgcgaacg ccctggtctt
      481 ctgatcttca aggctttgtt tacgggattc aatgttcttt gcgcattcct gccgggcctg
      541 ttcgacatcc gcggaacact gtctttcaac ggcaacaatc cgggtgatca cctcatccat
      601 gcctgcaccc cactgatttg cctgctgaat ggttgacgtt tgttttttct tctgcctgga
      661 tcgatccgga tgaaaaacgt accgctggac tttgcgcccg agagatttgc gtgcttgcag
      721 tgtttggtga aattataacg gttcaaattt tacatgtcaa cgaaaatata gataaaaata
      781 aggataggga tgccttgatt ttacttcaca atgccgttgt cgtccccgtt caaagaattg
      841 atttgcccca aacggccagg cagggtaaaa gaaacaacat gttcacggga gattgaatgg
      901 acgaatattt acacgttccc cgcggcgggg aaatcagctt tatggccgtc acgctgaaag
      961 tgactgccga aggatgcctg gactgcggca atcggcaact cctgtatgtc gaggggctca
     1021 ccaccgtcgg aagcgtctgc tgcggcgcgg tggagtgccg ggtcatttat gttcccgggt
     1081 tcatcgtctc ccggcattgc aaaacggatg aagcgtcggg acatcctgta agccgggtgg
     1141 agccggttac gaatcccgcc gcacttgacg aggtgaaaaa gctgctcgtc aaaaccttcc
     1201 cttcgtctct tttccttttt gtctgagaaa aaactatgcg gcgccttggc ggcttgcggg
     1261 aaaacgccgc gcaacccagg tggccgcaac atcttcgatg attccgtaaa tcacgccggc
     1321 cgccatgagc agaaccgcgc ccagtttgaa gggcccgaaa aggggcatca tggcaagctg
     1381 cagggtcatc agagcgccga ttcccgcccc gcgcagcgcc gggtgaaggt caatgccgtc
     1441 cgccaggccg atcacaaagc cctgcgtggt gcggcctgcg accagcatgg ccagccacca
     1501 cgcttccggc ccggaggtca gtcccgcggg cttgagcgcc ggaatataca ggcttgcggc
     1561 gcagtagcat cccgtgagca ggccgatg
//
LOCUS       DAOSIM010000353         1587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_67665_length_1587_cov_10.030026, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000353 DAOSIM010000000
VERSION     DAOSIM010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67665_length_1587_cov_10.030026"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..304
                     /locus_tag="PLR47_10840"
     CDS             110..304
                     /locus_tag="PLR47_10840"
                     /inference="COORDINATES: protein motif:HMM:NF014009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HOG13491.1"
                     /translation="MFKDEPGLHADHEDWLERLAPHAPTSFYRHNRTGEDNGDAHLKR
                     QVMGREVVVAVTKGRLDFGP"
     gene            complement(389..823)
                     /locus_tag="PLR47_10845"
     CDS             complement(389..823)
                     /locus_tag="PLR47_10845"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG13492.1"
                     /translation="MHKNRVWAGIALLILMPLWAWAGEYNAGVQSNIILRAETMSNGE
                     PIDYLDTDRPQVTVMTVDIAPGAGTGWHSHPMPVYAYVMAGQLTVEIERGKTAEFREG
                     DAIIEVVNLRHNGTNHGKVPVKLLVFYLGAKDVPNVIKGNRP"
     gene            complement(823..1476)
                     /locus_tag="PLR47_10850"
     CDS             complement(823..1476)
                     /locus_tag="PLR47_10850"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG13493.1"
                     /translation="MSKLADIIFFRQHGICPRWFCFTFDNGLRRLVQNPDRIIRPYIG
                     EGNKVLDVGPGIGYFTIPIARRAGSGGKVIAADIQESMLRGIAKRAERAGVSGQVELY
                     LSQPDEIYSGEPVDFILSFWMAHEVPRLDRFFRQLRAALKPAARFLLAEPALHVSRAQ
                     FDGEIDLAQKAGLVLLDRPAISLSHGALFGSSPAVAASESVSADRMAESPVSGKDDR"
BASE COUNT          365 a          467 c          423 g          332 t
ORIGIN      
        1 gtgctccatt tggaatggcc ggacataggg tactctcctg acaaaaaagt tgatcctgca
       61 tattttttcg cctcatagca taaggcgata agcttgtaaa gttaattaca tattcaaaga
      121 tgaaccgggt ctccacgcgg accatgaaga ctggctggag cggctggcgc cgcatgcgcc
      181 gacgtcgttc taccggcata accgcaccgg cgaagacaac ggcgacgcac atctgaagcg
      241 tcaggtcatg ggaagggaag tcgtcgtggc cgtgaccaaa ggcaggctgg acttcggccc
      301 ctgagagcag attttctacg gcgaattcga cggcggccgc agaaaacgcg tgatggtgaa
      361 aatcatcgga gagtaggaaa acccgccgtc agggccgatt tcctttgatg acattgggaa
      421 cgtcttttgc cccgaggtag aagaccagga gcttgaccgg aacctttcca tgattggttc
      481 cgttgtgcct gaggttgacg acttcgataa ttgcgtcgcc ttccctgaac tcggcggtct
      541 ttcctctttc gatctcgacg gtcagttgtc cggccatgac ataggcataa acgggcatgg
      601 ggtggctgtg ccagcccgtg ccggcgccag gcgcgatgtc caccgtcatg acggtcacct
      661 gaggccggtc cgtgtccaga taatcgatcg gctccccatt gctcatcgtt tccgcccgaa
      721 gaataatgtt gctttgcaca ccggcattgt attctccggc ccaagcccac agaggcatca
      781 gaatgagcaa agcgattcct gcccacaccc gatttttatg cattatcgat catcctttcc
      841 gctcaccggc gactctgcca tccgatccgc ggaaacgctt tcacttgcag cgaccgccgg
      901 actgcttcca aacaacgcgc cgtggctcaa agaaattgca ggacgatcca agagcactag
      961 gccggccttt tgcgccagat cgatttcccc gtcaaactgc gcccgggaca catgcagtgc
     1021 gggttcggcc agaaggaagc gagccgcggg cttgagggcg gctcgcaact gacggaaaaa
     1081 ccggtccagc cggggaactt catgcgccat ccagaaggac agaataaagt ctaccggctc
     1141 ccccgaataa atttcgtcag gttgggaaag ataaagctcg acttggccgc tcacccccgc
     1201 ccgttcggcg cgctttgcaa tgccccgcag catcgactcc tgaatatcgg cggcgataac
     1261 cttcccgccc gagcctgcac gccgggcaat cggaatcgta aaatacccga tgcccggccc
     1321 cacatccagc accttgttcc cttccccgat gtaaggacgg atgatgcggt ccggattttg
     1381 aaccagtcgg cgcagcccat tgtcaaacgt gaagcagaac cagcggggac aaatcccgtg
     1441 ctgtcgaaaa aaaataatat cggccagttt gctcactgcg cctcctaatc ttttctgtcc
     1501 cgctacccgg atttcaggca acggcaagtc ccctgctttt cagaaagtct ttgacgtcct
     1561 gaattttgag aagccggaaa tgaaaaa
//
LOCUS       DAOSIM010000354         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_67720_length_1586_cov_4.290660, whole genome shotgun sequence.
ACCESSION   DAOSIM010000354 DAOSIM010000000
VERSION     DAOSIM010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67720_length_1586_cov_4.290660"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PLR47_10855"
     CDS             complement(<1..730)
                     /locus_tag="PLR47_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOG13494.1"
                     /translation="MKPWAAHYPEHVPQMMAYPRIPVFRFLTDTAAAHPDDAAMTFND
                     VSTTYRELNDKVNRFARVLAQAGVRKGDRVALILVNSPTYVIAFFAVLKLGALAANLS
                     VGIAGEELASCLRNAGARVAVTLDLFAQNLYRVIGQTAVETVILHSVFGLEKKIAVEP
                     GRPPIRIFNDLLSAVDRADEPQIDVFPEDAAVLQYTSGSTGTPKAATLTHANMVASVL
                     QTDAWIGIDKAGNAAVMCLIPFFHV"
     gene            883..1005
                     /locus_tag="PLR47_10860"
     CDS             883..1005
                     /locus_tag="PLR47_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13495.1"
                     /translation="MKNTGFRGGRLTRKTMSLAADVAIFGTSDGSTGNMQGKRR"
     gene            complement(1260..>1586)
                     /locus_tag="PLR47_10865"
     CDS             complement(1260..>1586)
                     /locus_tag="PLR47_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOG13496.1"
                     /translation="KKRCPVRPHDPGRPGGVGVEIYKDVSLRMAPLKSRDVDNMLEEL
                     TARKLLAGYRGAEPVNLAALKKTLLSFSKLMMDLQDRVESVDLNPVMCTAKSCTVVDS
                     RIMLKK"
BASE COUNT          350 a          467 c          442 g          327 t
ORIGIN      
        1 agacatggaa aaagggaatc aggcacatga cggccgcgtt gccggccttg tcaatgccga
       61 tccaggcatc ggtctgcaaa acgctggcga ccatattggc gtgggtcagc gtcgccgcct
      121 tgggcgttcc cgtcgagccg ctggtgtact ggagcaccgc cgcgtcttcg ggaaagacgt
      181 cgatttgcgg ttcatccgcc cggtccaccg cagacagaag atcgttgaaa atccgaatgg
      241 gcggacgccc cggctccacg gcaatctttt tttcgaggcc gaaaaccgaa tggagaatca
      301 cggtttccac agcggtttgt ccgatgaccc ggtaaagatt ctgggcaaac agatccagcg
      361 tcaccgccac ccgggcgccc gcattgcgca ggcaggaggc cagctcctcc cccgcgatgc
      421 cgacgctcag attggcggcc agagcgccca gtttgaggac ggcaaaaaag gcgatcacat
      481 aggtgggcga atttaccaga atcagggcca cccggtcgcc tttccggacg cccgcctgcg
      541 ccaggacgcg agcaaaccgg ttgaccttgt cgttgagttc ccggtacgtc gtggaaacat
      601 cgttgaaggt catcgccgcg tcgtccggat gcgcggccgc cgtgtccgtg agaaatcgga
      661 aaacaggtat ccgcggatac gccatcatct gcggaacatg ctcgggataa tgcgctgccc
      721 agggtttcat gttcattttt tttcaacctg aaaggaattg ggaaacgatg ttattttaac
      781 acagaccggc tccggcgaca aatgatcgct tcagcccccg actacgacat atggtattta
      841 atttcttgac agcatctaca tatagtggta aggtgcagac caatgaaaaa caccggtttc
      901 cggggcggaa gattgacccg gaaaaccatg tcgctggcgg cggatgttgc aatcttcgga
      961 acctccgacg gctccactgg gaatatgcag ggaaaaaggc gataaagtgt taaccacctc
     1021 aacaaacctt tagccacggg cggaccggca acggctcgcc ctttttgatt ccggatccat
     1081 accgaagttc atccttcgga aacaggcaca tcatttgaat caattgcgtc agacgcctgc
     1141 aaccttttaa acttctttat gtgcctgccc cgcgcttcat tttgaaaaat taaagcgatc
     1201 ttcgccttcg gaaccggctc cgggcgcgct tcgggccgca ccgccctccc gcgcgcaatt
     1261 tactttttaa gcatgatccg ggaatcgacg acggtgcagg acttggcggt gcacatgacc
     1321 ggattgagat cgacggactc cacgcggtcc tgcaaatcca tcatcagctt cgagaaactc
     1381 agcagcgtct ttttgagcgc ggccaggttg accggctccg cgccgcggta ccccgcaagc
     1441 agcttgcgcg cggtcagctc ttccagcatg ttgtcgacgt cgcgggattt cagcggggcc
     1501 atgcgcagcg acacgtcctt gtaaatttcc acgccgacgc ccccaggccg accaggatca
     1561 tggggccgaa ctggacatcg tttttt
//
LOCUS       DAOSIM010000355         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_67887_length_1583_cov_10.279450, whole genome shotgun
            sequence.
ACCESSION   DAOSIM010000355 DAOSIM010000000
VERSION     DAOSIM010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67887_length_1583_cov_10.279450"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="PLR47_10870"
     CDS             complement(<1..1049)
                     /locus_tag="PLR47_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13497.1"
                     /translation="MKNVIMFLCAVLALGCFPVSVQSAIAAEPSVYVDYEYGDLKEVI
                     VGLPYGMSPSLEAKWFADALKVLPPDEADYARKTAGMLWTEMINPETGKSETRMLEEE
                     NQAFIGILKSLGVKVYRPTEITVDFIKEHYGADVLLNGFSQDFPRDNMAVIGTNVIEF
                     NLRTPIRKADISGFRNILSEKCSDPAVKWFSMPHTELLKPARPDTPFLEGGDVIVLGK
                     TVLVGNTQNPSVGSNEAGCRWLKNILGDQYQVKRVRLVESVLHLDCVLSVPREGLAII
                     CEEAFVDGLPKELKGWDLIRVSLKDVQRLAVNGVPVNSENYILSYNRHNDNRAIQAEL
                     EKRGIRVHRVFFGTH"
     gene            complement(1471..1557)
                     /locus_tag="PLR47_10875"
     tRNA            complement(1471..1557)
                     /locus_tag="PLR47_10875"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1521..1523),aa:Leu,seq:caa)
BASE COUNT          374 a          436 c          391 g          382 t
ORIGIN      
        1 ttgtgcgttc cgaaaaacac gcggtggact ctgatgcccc gcttttcgag ctcggcctgg
       61 atggcgcggt tgtcattgtg ccggttatac gacaaaatgt agttttcgga attgacggga
      121 acgccgttta cggccagccg ctgaacatct ttgagactca ctctgatcag gtcccagccc
      181 ttgagctcct tgggcaggcc gtcgacaaag gcttcttcgc agatgatggc gagcccctcg
      241 cgcggtacgg aaagcacgca atccagatgc agcacgcttt ctacaagccg aacgcgtttc
      301 acctgatatt gatcgcccaa aatatttttc agccaccggc aacctgcctc attggatccc
      361 acgctgggat tttgcgtatt gccgactaaa acggtcttgc ccagaacgat gacgtcgccg
      421 ccctcgagaa acggcgtgtc cgggcgggcg ggtttaagca gctccgtgtg cggcatggaa
      481 aaccatttga ccgccgggtc ggaacatttt tcggacagaa tattccggaa tccggatatg
      541 tccgccttgc gaatcggcgt gcgcaaattg aattcgatga cgtttgtgcc gatgacggcc
      601 atgttgtcgc gggggaagtc ctggctgaaa ccgtttaaaa gaacatccgc gccgtaatgt
      661 tccttgataa aatcgacggt gatctccgtc ggacggtaaa ccttgacgcc cagagacttc
      721 aatatcccga taaaggcctg attttcctcc tccagcattc gcgtctcgct tttccccgtt
      781 tcaggattga tcatctccgt ccagagcatc cccgccgtct tccgggcgta atccgcttca
      841 tctggcggca gtactttcag cgcgtcggca aaccattttg cctccaaaga cggcgacatg
      901 ccgtacggca ggccgacaat cacctctttc agatcgccgt attcgtaatc cacgtaaaca
      961 gaaggttccg ccgcaatcgc cgattggaca gaaaccggga aacatccaag cgcgagaacc
     1021 gcgcataaaa acatgatcac atttttcatc acatctctcc gtcttaggag aaaaagagcg
     1081 tccttcctcg gttatcgtcc ggcaagtcgg gaactcccct cccgcccaat tattttacct
     1141 ctccggcctg aatgcataac tctcgcttcg catttttgca aatgacctct gcgcttttcc
     1201 tgaccgaaag aaaatttttc caaaagggtt cggcgaaaaa gaaaagctgg acggttaaat
     1261 tcttttcagg gccgcttcct gcaaataata cgataaagtt tatcaataaa ccgtaattat
     1321 tcaaagactc tacatttatt agccatggtg gcgtaaaaag gatttaacaa ttgcagctaa
     1381 caataacctt tcccgatgag gcgctgcttt tttctctggg tggcccgctg ctggaaagtc
     1441 ctaagacagg agagatcaag gcattagata tggtgccgga gcccggaatc gaaccgggat
     1501 gcccttgcgg gcggaggatt ttgagtcctc tgcgtctacc agtttcacca ctccggcacg
     1561 ggcgcgatta tatagacgga agg
//
LOCUS       DAOSIM010000356         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_67891_length_1583_cov_8.683246, whole genome shotgun sequence.
ACCESSION   DAOSIM010000356 DAOSIM010000000
VERSION     DAOSIM010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67891_length_1583_cov_8.683246"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..1423
                     /gene="rimO"
                     /locus_tag="PLR47_10880"
     CDS             47..1423
                     /gene="rimO"
                     /locus_tag="PLR47_10880"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOG13498.1"
                     /translation="MKKLLAGKLHIISLGCSKNLIDSEVMGALARDSGMTMANRPQDA
                     DVVLVNTCGFIGPAKEESLEAILALAEIKKQRGGAIRLVVAGCLAQRYGRKLLSEIPE
                     ADLWIGTGEVGRIAEHLQGLQAGGAKRKAIVSRPDFLMTAHHKRVLPSGAATAYLKIS
                     DGCSNRCTYCAIPSIRGRARSRRPEDIVLEAENLVARGVREIILIGQDTTAYGKDLKN
                     RPLLSGLLTDVSAVSGLRRLRLLYAHPAHVTTGVLETMASRENICRYLDLPIQHIDDA
                     ILAAMNRRVSSKTIREIIRQARGMMPDLALRTSLIVGFPRETPKRFDRLMDFVRNMRF
                     DHLGVFTYSREEGTPAADLPSRISEKEKERRRDAVMNEQADISLAVNQTLIGSIQEVL
                     IEGKSERRGFALVGRCRRQAPDVDGVTYLKKGRAAAGDFVRCKITGCDHYDLFGEILF
                     DNCRKMKL"
BASE COUNT          338 a          478 c          467 g          300 t
ORIGIN      
        1 ccgacggcgc gaaaccccgc aaggttctga ttcggaaact tccgcagtga aaaaattgct
       61 tgccggaaaa ctgcacatca tctctttggg ctgctccaag aacctgatcg attcggaagt
      121 catgggggct ctggccaggg actccggaat gacgatggcc aatcgtcccc aggacgctga
      181 tgtcgttctg gtcaacacct gcggctttat cgggccggcc aaggaagagt ctctggaagc
      241 gattctggcg ctggcggaaa tcaaaaagca gcgcggcggc gccatcaggc tggtggtggc
      301 cggctgtctg gcccagcgct acggccgaaa gctgctttcc gaaattcccg aagccgacct
      361 gtggatcggc acgggagaag tcggacgcat cgccgaacat ctgcagggtc tgcaggcggg
      421 cggggccaaa cggaaggcga ttgtttccag gccggatttt ctgatgacgg ctcaccacaa
      481 acgggtcctg ccttccggcg ccgccaccgc ttatttgaaa atatccgacg gctgctccaa
      541 ccgctgcacc tactgcgcca ttccctccat ccgcgggcgg gcgcgaagcc gcaggccgga
      601 agacattgtg ctcgaagcgg aaaacctggt cgcccgcggc gttcgggaaa tcattctgat
      661 cggccaggac accaccgctt acggaaaaga tttaaaaaac cggccgcttc tctccggcct
      721 gctgaccgat gtgtcggcgg tttccggcct gcgccggctg cggcttctgt atgcgcatcc
      781 ggcgcacgtc acgaccggcg ttctggaaac catggcctcg cgggaaaaca tctgccgcta
      841 cctcgatctg cccattcagc atatcgacga cgcaatcctt gccgccatga accgccgcgt
      901 ttcttcaaag accattcggg aaatcattcg tcaggcgaga ggcatgatgc ccgaccttgc
      961 gcttcgaacc tccctcatcg tgggttttcc gcgggaaacg ccaaagcggt tcgaccgcct
     1021 gatggatttt gtgcggaata tgcggttcga ccacctcggc gttttcacct attcgcggga
     1081 agaaggcacg cccgccgcgg accttccgtc gcgcatctct gaaaaagaga aggagcgaag
     1141 gcgagacgcg gtcatgaacg aacaggcgga catttccttg gccgtgaacc aaacgctgat
     1201 cggctccatc caggaggtgc tgatcgaagg gaaaagcgag cgccggggat tcgcgctggt
     1261 cgggcgctgc cgccggcagg cgccggacgt cgacggggtc acctacctga aaaaaggccg
     1321 ggccgccgcc ggcgatttcg tccgctgcaa aatcaccggc tgcgaccatt acgacctttt
     1381 cggagaaatc ctgtttgaca attgccgaaa aatgaaacta tagtcttttc acgaacgaac
     1441 ccggagcagg atcatggatc tggcggatgt tttttccctt tacaaagaag agatgagcag
     1501 ggtggaaacc cacctggacc ggtacattac atccgacgtc cggctgattc ccgacgtcgc
     1561 gcatcacctg atcgacagcg gcg
//
LOCUS       DAOSIM010000357         1582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_67965_length_1582_cov_6.115914, whole genome shotgun sequence.
ACCESSION   DAOSIM010000357 DAOSIM010000000
VERSION     DAOSIM010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67965_length_1582_cov_6.115914"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="PLR47_10885"
     CDS             complement(<1..1125)
                     /locus_tag="PLR47_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG13499.1"
                     /translation="MKLYKFTESYRLFEEAQQYVPNGIYGPRTPAFLTFGSYPAFIRR
                     GEGCRIWDVDGNEYIDYMCSFGTNLLGLKHPKIDAAAKKQWENADCFTLPSDLWLPLA
                     KKIAGTIHGADWCVFGKNGSDVTSYAITLARIFTGKQDILLAHGSYHGSHFWCQPHGQ
                     GVPEEWKTHVHYFEYNNAKDLERVVSEHEGAVAGIMLTPHRHDSMRDQELPAKEFVDA
                     VNALAKKHRFLILVDDVRCGFRLELAGSSMHYGFNADIQCFGKAMANGYAISVAAARK
                     EMMDAAKSIFFTGTHYFSGVPFAAALACIDEIQASGAIEYIRKTGTQLMDGLRQAANA
                     AGLQVSVTGPPAMPYMTFADDPLFEKNRFFCGEAARRGIFF"
     gene            complement(1154..>1582)
                     /locus_tag="PLR47_10890"
     CDS             complement(1154..>1582)
                     /locus_tag="PLR47_10890"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HOG13500.1"
                     /translation="AAILSICTLGIACSFVPRVRNIKMTYQAGNYFILIFCLVVSSMA
                     DFNRLVSTAPIMLVYVTFTILVCLALHVALAKIFRIDADTVIITSVAGICSPPLVPMV
                     ASALKNKEIVLSGVITGIIGWVIGTYLGISLSYILRGIGA"
BASE COUNT          369 a          441 c          444 g          328 t
ORIGIN      
        1 gaaaaagatg cctcgccgcg ccgcctcacc gcagaaaaac cggtttttct caaaaagggg
       61 atcgtcggca aaggtcatgt agggcatggc cgggggaccg gtgacgctga cctgcaggcc
      121 cgccgcgttc gcggcctggc gaaggccgtc catgagctgc gtccccgttt tgcgaatgta
      181 ctcgatggcg cctgaagcct gaatctcgtc gatgcaggcc agcgccgcgg caaagggaac
      241 gcccgagaag taatgtgtgc cggtaaaaaa aatgcttttc gccgcatcca tcatttcttt
      301 gcgcgcggcc gccacggaaa tagcgtatcc gttggccatc gccttgccga agcactggat
      361 atcggcgttg aatccgtaat gcatggagct tcccgccagt tcaaggcgga aaccgcagcg
      421 gacatcatcg acaaggatca gaaaccggtg ctttttcgcc agggcgttga cggcgtccac
      481 gaattccttt gccggcagct cctggtccct catggaatcg tgccggtgcg gcgtcagcat
      541 gatgccggca acggcgcctt cgtgctccga gacaacgcgc tccaggtctt tggcgttgtt
      601 gtattcaaag taatggacgt gcgtcttcca ttcttccgga accccctggc cgtggggctg
      661 acaccagaag tgggatccgt ggtaagaccc gtgcgccagc agaatgtcct gcttcccggt
      721 gaaaatccgg gccagcgtga ttgcgtacga cgtcacatcg gagccgtttt ttccgaaaac
      781 gcaccagtcc gcgccatgga tcgttcctgc gatttttttc gccagcggaa gccacagatc
      841 ggacggaagc gtgaagcagt ccgcattttc ccactgtttt ttcgccgccg cgtcgatctt
      901 cgggtgcttc aaccccagaa gattggtccc gaatgaacac atgtaatcga tgtattcgtt
      961 tccgtccaca tcccagatcc ggcatccctc tccgcgccga ataaaggcgg gataggaccc
     1021 gaaggtgaga aaggccgggg tgcgggggcc gtaaatgccg ttgggaacgt attgctgcgc
     1081 ttcctcgaaa agccggtacg attcagtgaa cttataaagc ttcattgtag cctcccatca
     1141 taaaatatac cgattaagcg cctattccgc gcaaaatata agacagcgat attccgaggt
     1201 aggttccgat gacccagccg atgatgccgg tgatgacccc ggaaagcacg atttccttgt
     1261 tcttcaaagc cgaggcgacc atcggaacga gcggaggcga gcaaattccc gcaacggagg
     1321 tgatgatcac ggtgtccgcg tcgatcctga atattttcgc caatgccaca tgcagcgcaa
     1381 gacagaccaa tatggtgaat gtgacataaa ccagcatgat cggagcagtg gacacaagac
     1441 ggttgaagtc cgccatggag cttaccacaa gacagaagat cagaatgaaa taattgcctg
     1501 cctgataggt catttttata ttccggactc ggggaacaaa cgaacaagcg atccccagcg
     1561 tgcatatgga aaggatggcc gc
//
LOCUS       DAOSIM010000358         1580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_68095_length_1580_cov_8.414426, whole genome shotgun sequence.
ACCESSION   DAOSIM010000358 DAOSIM010000000
VERSION     DAOSIM010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68095_length_1580_cov_8.414426"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="PLR47_10895"
     CDS             complement(<1..1272)
                     /locus_tag="PLR47_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG13501.1"
                     /translation="MKIAEKTMNDVFRNRVQKYQDRLAVEKKMNGVWHTATWNEYYER
                     SRAVGMGLYALGIRKGDMVSILSENRLEWIYTDMGALGIGACVIPIYPTLTAEEIEYI
                     LNNSESKVIVTEDRGQLKKVLAVIERCPGIEKIVVMEEKDAQGHPGVMSFTALMELGR
                     KKHAEDPSLFEKLSREVTVDDLATLVYTSGTTGLPKGAMISHGNVFWIVKSLDAVQPP
                     FADDQDVLVAFLPLSHVFQRIPGHFYAMHRGITISYAQSIDTLLTDFEEKRPTMILAV
                     PRVCEKVYQKIITQVKEQSPFKQKVFYWGQCVGNRISELRENHKPIPPGLRLKYKIAY
                     AVIFKKLQQKLGGRLKWMTAAGAPTAPEIIRFFNAAGITVIQGYGMTETTAPASIQSL
                     SDFRIGTTGKPMPGQDIRIAEDGEILIKGGNV"
     gene            complement(1352..>1580)
                     /locus_tag="PLR47_10900"
     CDS             complement(1352..>1580)
                     /locus_tag="PLR47_10900"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOG13502.1"
                     /translation="TTCGVGTDQYPCIMQSCILGGHMRVGLEDNIRMPNGALAKGSYE
                     QVEVAVNIANCLGRPVATTDEARLIMGLRKR"
BASE COUNT          301 a          477 c          429 g          373 t
ORIGIN      
        1 cacgtttccg cccttgatca gaatctctcc gtcttcggcg atcctgatgt cctggccggg
       61 catcggcttg ccggtcgtgc cgatgcgaaa atcggacagg gactggatgg acgccggcgc
      121 cgtcgtttcg gtcatgccgt atccctgaat gaccgtaatt ccggcggcgt tgaagaaccg
      181 gataatttcc ggcgccgtgg gcgcgccggc agccgtcatc catttcaacc gtccgcccag
      241 cttctgctgg agctttttaa agatgacggc ataggcgatt ttgtatttca gtctgagccc
      301 cggcggaatg ggcttgtgat tctcgcgaag ttcgctgatc cggttgccga cacactgccc
      361 ccagtaaaac accttctgct tgaaagggga ctgttccttc acctgcgtaa tgatcttctg
      421 gtacactttt tcacacacgc gcggaacggc cagaatcatg gtcggcctct tttcttcgaa
      481 gtccgtcaga agcgtgtcga tcgactgggc ataggagatg gtgatcccgc ggtgcatggc
      541 ataaaaatgt ccgggaatcc gctgaaacac gtgggagagg ggaagaaagg caaccagcac
      601 gtcctgatcg tcggcaaacg gaggctggac cgcgtccagg gatttcacaa tccagaaaac
      661 gttcccgtgg ctgatcatgg cgcccttggg caagccggtc gtaccggacg tatatacgag
      721 agtagccaga tcatcgaccg tcacttcccg ggagagcttc tcaaaaaggg aagggtcttc
      781 ggcatgcttt ttccttccca gttccatcag cgccgtaaag ctcatcacgc cgggatgccc
      841 ctgagcgtcc ttttcctcca tcacgacgat cttttcaatg ccggggcacc tctcaatgac
      901 ggccagcact tttttgagct ggccgcggtc ttccgtcaca ataacctttg attcggaatt
      961 attgaggatg tactcgatct cctcggccgt cagcgtcgga tagatcggaa tcacgcaggc
     1021 gccgattccc agcgcgccca tgtcggtgta gatccattcc agccggtttt cggacagaat
     1081 ggacaccatg tctcctttgc gaatccccag ggcatacagc cccatgccga cggcgcgcga
     1141 ccgctcgtag tattcgttcc aggtggcggt gtgccacacg ccgttcatct ttttttcaac
     1201 ggcaagacgg tcttgatatt tttgaacgcg gttcctaaaa acgtcgttca tggttttttc
     1261 tgcgattttc attgcgcgtt ctcccgaatc gatccagagc ggttcgccgc aggcgccctt
     1321 gtccggcatc cgcggcgaac cacccgtttt tttaccgctt cctgagcccc ataatgagac
     1381 gagcctcgtc cgtggtcgcc accgggcgtc ccagacagtt ggcgatgttc accgccacct
     1441 ccacctgctc gtaactcccc ttggccagcg ccccgttggg catccggatg ttgtcctcca
     1501 gaccgactct catgtgcccg ccgagaatgc aggactgcat gatgcaggga tattgatccg
     1561 tcccgacgcc gcaggtggtg
//
LOCUS       DAOSIM010000359         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_68163_length_1579_cov_5.334646, whole genome shotgun sequence.
ACCESSION   DAOSIM010000359 DAOSIM010000000
VERSION     DAOSIM010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68163_length_1579_cov_5.334646"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PLR47_10905"
     CDS             <1..394
                     /locus_tag="PLR47_10905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG13503.1"
                     /translation="VAENIVFTGVLDREKLTDLYLAGDMYLMLSKFDTFGMVVLEAMA
                     AGLPVMVSSRVGAKDVVVQGENGFVIENPSDHDHVASVLKMMLDEGNRRRMSAAALAT
                     ASENSWDAATEKYRSLYTEILAAKKKEG"
     gene            399..1517
                     /locus_tag="PLR47_10910"
     CDS             399..1517
                     /locus_tag="PLR47_10910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOG13504.1"
                     /translation="MRIIDPSSKFKLPGFVRKVLLIQLGDIGDVVWTTPSIRAARSAV
                     PDGKVSVMVREGFGDLLEADPAIDRVFEAKHYRGNLLRQAASQLSFLRDLRAERFDLA
                     VDLRLGDRGAFMAFATGAPIRVTLYYPKNIPFWRNYLFTHGVEVGPPVHPFGAAEQSL
                     RILRKLGIDTDDVTPGLWVADSVNGRVREILAREKMNGSKGWITVNPFSRWPYKEWDT
                     LKWIEVINWLGRNYAIPVIIVGSREEQAKAEDIRRQCKSGTFNFAGLTTLAELAGLLG
                     QSLLHIGVDSAAPHIAAATGAPTITIYGPSSWKEWAPVGKSHRVIVSNMDCVPCRQKG
                     CDGSGRSRCLETLTVDQVKPVIRKAMDELLPKIHPIED"
BASE COUNT          352 a          430 c          465 g          332 t
ORIGIN      
        1 cgtggccgaa aatattgttt tcaccggcgt gctcgaccgg gaaaagctga cggacttgta
       61 cttggcgggt gatatgtacc tgatgctttc aaagtttgac acgttcggca tggtcgttct
      121 cgaggcaatg gcggccgggc tcccggtcat ggtcagcagc cgcgtgggcg cgaaagatgt
      181 ggtggtccag ggtgaaaacg gtttcgtgat cgaaaacccg tcggaccacg atcatgtggc
      241 ctccgtcctg aagatgatgc tggatgaagg caaccgccgc cggatgtccg ctgccgcact
      301 ggcgacggcg tccgaaaact cctgggacgc ggcgaccgaa aagtaccggt cgctctacac
      361 agaaattctg gcggccaaaa agaaagaggg ttgagggcat gcggattatc gatccatcat
      421 caaaattcaa acttcccggc ttcgtccgca aggttcttct gattcaactg ggggacatcg
      481 gcgacgtggt ctggacgacg cccagcatcc gcgcagcccg cagcgccgtg cccgacggca
      541 aagtatccgt gatggtgcgg gaaggcttcg gcgaccttct ggaagcggac cctgcgatag
      601 accgtgtatt cgaggctaaa cattatcggg gaaatctgct gcggcaggcg gcaagccagc
      661 tctctttttt acgagatctc cgcgccgagc gcttcgatct ggccgtggat cttcgcctgg
      721 gcgaccgggg cgccttcatg gcgtttgcaa cgggagcgcc aatacgggta actttgtatt
      781 atcccaagaa cattcccttc tggagaaatt atttatttac gcatggcgtc gaagtcggtc
      841 caccggttca tcccttcggc gccgcagagc aatcgctgcg aatcttaaga aaactcggca
      901 ttgatacgga tgacgtcaca cccggcttat gggtggcgga ttcagtcaat gggcgtgtcc
      961 gggaaatcct ggctcgtgaa aagatgaatg gctcgaaggg ctggatcacc gttaatccat
     1021 tttcccgttg gccttataag gaatgggaca ccctcaagtg gatcgaagtc atcaactggc
     1081 tggggagaaa ttatgcaatc cccgtgatca tcgtcggctc ccgggaggaa caggcaaaag
     1141 cggaagacat tagacgtcaa tgcaagtccg ggacattcaa ctttgcaggc ctgacaactc
     1201 tggccgagtt ggccggcctg ctcggtcaaa gcctgcttca tatcggtgtg gacagcgccg
     1261 cgccccacat cgcggcggcc accggcgcac cgacgatcac catctacggc ccgtcctcct
     1321 ggaaagagtg ggcgcccgtt ggcaaaagcc accgggttat tgtatcgaat atggactgtg
     1381 tcccctgccg ccagaagggc tgcgacggaa gcggacgcag ccggtgtctg gaaacgttaa
     1441 cggttgatca ggtcaaacct gtgattcgta aagccatgga tgagctcctg ccgaaaatcc
     1501 atccgattga agattgaaat tcccgccttc ccgctatgaa atgtacgagc gcattttggt
     1561 cttgcccaac aaatagacc
//
LOCUS       DAOSIM010000360         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_68217_length_1578_cov_7.209455, whole genome shotgun sequence.
ACCESSION   DAOSIM010000360 DAOSIM010000000
VERSION     DAOSIM010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68217_length_1578_cov_7.209455"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PLR47_10915"
     CDS             <1..520
                     /locus_tag="PLR47_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13505.1"
                     /translation="RSDVFMIGCASDRAQLEALIEDEHCEYRWILGGGDLVVERDFTV
                     EKMRIDGEDIPITRAAKTARGYEVWCGSEKLKSKINTDVKIEMEILTKKAKSNRSFPV
                     YLLYPTRGLEINFHFGQAGLKNVRAETFFAGRHPRADIRASRDQSIHIRIAPEEWVFP
                     TSGVIFIWDVSS"
     gene            complement(530..1441)
                     /locus_tag="PLR47_10920"
     CDS             complement(530..1441)
                     /locus_tag="PLR47_10920"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG13506.1"
                     /translation="MKGNRLPWPIDLAGFYWRRKIRGQSVPLLASFKVTHRCNLSCRA
                     CPFHLHADQSHMPRDTAVHALEALRRLGTRIVVFEGGEPLLWRDGGYRLHDLVAYARR
                     RFLRVAVTTNGTLPLDVPADTLWVSLDGWKETHDELRSGSFDAVYSRLKETGHPRVFV
                     HCTINRINWRDLERMAAQVRSLPAVRGMTFQFFYPYGLGEDDLALAPDERRAAVETLL
                     DLKKRGFPILNSAGSLAAMTQKRRSCRDDILINVDPDGTITRGCYVKSRGTVNCDACG
                     FTPVAEASGALDLMPGSLWAGWKLFLK"
BASE COUNT          307 a          478 c          494 g          299 t
ORIGIN      
        1 ccgcagcgac gtgttcatga tcggctgcgc ctcggaccgg gcgcagctcg aggcgctcat
       61 tgaggacgag cactgcgagt accgctggat tctcggcggc ggcgacctgg tggtggagcg
      121 ggatttcacc gtggaaaaaa tgcgcatcga cggcgaagac attccgatca cgcgggccgc
      181 caagaccgcc aggggctacg aagtctggtg cgggagcgaa aagctgaagt ccaaaatcaa
      241 cacggatgtg aaaattgaga tggagattct caccaagaag gccaagagca accggagctt
      301 tcccgtgtat ctgctttacc cgacgcgcgg cctggaaatc aatttccact tcgggcaggc
      361 gggcctgaag aacgtccggg cggagacctt ttttgccggc cgccatccgc gcgcggacat
      421 tcgggccagc cgcgaccagt ccatccatat caggattgcg cctgaagaat gggtctttcc
      481 gaccagcggc gtgatcttta tctgggacgt atcatcatag ggtcacccgt cacttcaaaa
      541 acaatttcca gcccgcccac agcgaaccgg gcatcaggtc cagtgctccg gaggcttcgg
      601 caaccggcgt gaagccgcag gcatcgcagt ttacggttcc ccggctcttg acgtagcagc
      661 cccgggtgat ggtgccgtcc ggatcgacgt tgatcaggat gtcatcccgg cagctccggc
      721 gtttttgcgt catggcggca agacttcccg cggagtttaa gatggggaag cctcgttttt
      781 tcagatccag cagcgtctcg acggcggccc ggcgctcatc cggcgccagg gcaaggtcgt
      841 cttcgcccag gccgtacgga taaaaaaact gaaaggtcat tcccctcacc gcggggaggc
      901 ttcggacctg tgcggccatc cgttccaggt ctctccagtt gatgcggttg atcgtgcagt
      961 ggacaaaaac acggggatgc ccggtttcct tcaggcggga ataaacggcg tcaaaggatc
     1021 cgctgcgcag ctcgtcatgc gtttccttcc atccgtcgag gcttacccag agcgtgtcgg
     1081 cgggaacgtc cagcggcagc gtgccgttgg tggtcaccgc cacgcgcaaa aatcgccggc
     1141 gggcataggc taccagatcg tgcagacggt atccgccgtc gcgccagagc agcggttccc
     1201 cgccttcaaa aacgacgatg cgcgttccga ggcggcgcag ggcctccagg gcgtggacgg
     1261 ccgtgtcccg cggcatgtgg ctttgatccg catgcagatg aaaggggcag gcgcggcagg
     1321 acagattgca gcggtgcgtc accttgaagc tcgccagcag cgggacggac tgcccgcgga
     1381 ttttccgccg ccagtaaaat cccgccagat cgatcggcca gggaagacgg ttgcccttca
     1441 tgatgtccgc gtctttcccg cgctacgaga tctcctcgat ccccagcgga tatttggaca
     1501 gaacctcgca gccgtctttc gtgatgacga tgtcgttttc gtagcggaag ccgatgttct
     1561 cctccggcgc ggcgtact
//
LOCUS       DAOSIM010000361         1560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_69298_length_1560_cov_6.756811, whole genome shotgun sequence.
ACCESSION   DAOSIM010000361 DAOSIM010000000
VERSION     DAOSIM010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69298_length_1560_cov_6.756811"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="PLR47_10925"
     CDS             complement(<1..765)
                     /locus_tag="PLR47_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13507.1"
                     /translation="MLKKTTVNQLSDLASALMEKGTLVAPVREDAGFQFREITDAKRV
                     DLNFHNTVISPKSVFFPQTEDFVRYRTGRPMQEAEPVEPQGKPLILFGVRPCDVKSFE
                     IMDIHFSGTGDVDPYWQARREAAAIIGYAFDLGAPAEPEEFYQTLGIGAADPEGSDIF
                     MVRRGDDLLLKSITGKGEALLTGMGGLAGASADDEKFFDDTVAAGRDFKTRFTCVDAE
                     VITKKLEDLFHNTDFWEKASNACLSCGVCTFACPTCY"
     gene            complement(778..>1560)
                     /locus_tag="PLR47_10930"
     CDS             complement(778..>1560)
                     /locus_tag="PLR47_10930"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG13508.1"
                     /translation="AVSFQQIEEKLKTEARRLLESGEVSLVLAWGKGYDEKHPMPYVA
                     KCPSDADGIVFNEYCTHNPARYLVRYPRGTKIAVAVKAADSRAVVQLIQEDKVKRRDL
                     VILGIPVYGIKNPKTGELIDGKTTCGLYNPVLYDVLIGEEVHGQPVSSPYAVLAEYEA
                     MDRDARFAFWKKELDRCIRCYACRKACPMYHLTRFHHLAGRCIDCGECSRACPVDIPL
                     YLFHKKVAKDCEEMFGQAAGMSIEDKPVLVNFRMEDSDKILE"
BASE COUNT          313 a          465 c          437 g          345 t
ORIGIN      
        1 atagcaggtc ggacacgcaa aggtgcacac gccgcagctc aaacaggcgt tggatgcctt
       61 ctcccagaaa tccgtgttgt gaaaaagatc ttccagcttc ttcgtgatga cttcggcgtc
      121 cacgcaggta aaacgcgttt tgaaatcccg ccccgcggcc accgtgtcgt caaaaaattt
      181 ctcgtcatcg gccgacgctc cggccaggcc gcccattccg gtcagcagcg cctccccctt
      241 gccggtgatg cttttgagga gcagatcgtc gccgcggcgc accatgaaga tgtcgctgcc
      301 ttcgggatca gccgcgccga tgcccagcgt ctgataaaat tcctccggct cggcgggcgc
      361 gccgaggtcg aaggcgtatc cgatgatggc ggccgcttct cttcgggcct gccagtaggg
      421 atccacgtct ccggtgccgg aaaaatggat gtccatgatc tcaaagctct tcacgtcgca
      481 gggacgcacc ccgaaaagaa tcaagggctt tccctgcggc tcgaccggtt cggcctcctg
      541 catgggcctg cccgtccgat agcggacgaa atcttccgtc tgcggaaaga aaacggattt
      601 ggggctgatg acggtattat gaaaattcag gtccacccgc ttcgcgtcgg tgatttcgcg
      661 aaattggaag ccggcgtcct cgcggaccgg ggcgaccagc gttccctttt ccatcagcgc
      721 ggaagccaga tcggataatt gattgacggt ggttttcttc agcacaacaa aagctcctta
      781 ttccaaaatt ttgtcggaat cttccatgcg gaaattgacc agcacgggct tgtcttcaat
      841 gctcatcccc gcggcctggc cgaacatttc ttcgcagtct ttggctactt tcttgtggaa
      901 cagatacaga ggaatgtcca ccgggcaggc gcgcgagcac tcgccgcaat cgatgcaacg
      961 gccggccaga tgatgaaacc gggtcagatg gtacatcggg caggccttgc ggcaggcgta
     1021 gcagcggatg caccgatcca attctttttt ccagaacgca aagcgcgcgt cccggtccat
     1081 cgcttcatat tcggccagca cggcgtaagg agatgagacc ggctgtccgt gcacctcttc
     1141 gccgatcagg acatcataca gcacgggatt atacaggccg caggtggtct tgccgtcgat
     1201 gagctcacct gtcttggggt ttttgatgcc gtacaccgga atgccgagaa ttaccaggtc
     1261 gcgccgcttg accttgtctt cctgaatgag ctgaaccacg gcccggctgt ccgccgcctt
     1321 gacggcgacg gcgattttgg ttccccgcgg ataccggacc agatatctgg ctggattgtg
     1381 ggtgcaatac tcgttgaaca cgatgccgtc cgcgtcggaa ggacacttgg caacataggg
     1441 catgggatgt ttttcgtcgt agcctttacc ccacgcgagc accaggctca cctcgcccga
     1501 ttccagaagc cttcgggctt cggtctttaa tttctcttca atttgctgaa agctgactgc
//
LOCUS       DAOSIM010000362         1553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_69771_length_1553_cov_7.544726, whole genome shotgun sequence.
ACCESSION   DAOSIM010000362 DAOSIM010000000
VERSION     DAOSIM010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69771_length_1553_cov_7.544726"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..657
                     /locus_tag="PLR47_10935"
     CDS             238..657
                     /locus_tag="PLR47_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG13509.1"
                     /translation="MASANILLVDDEEDFRFIFTRQVTRILQDLQLEFFEAGDGEEAL
                     ELLRTGVKPSIIILDYTMPRMSGTELLKTIDREHGDLVNVPRLVLSGYIGEDAIREVK
                     SLRCDYMEKSMNTKIFYQRFCRYLTDKLGLPPAEPER"
     gene            766..1419
                     /locus_tag="PLR47_10940"
     CDS             766..1419
                     /locus_tag="PLR47_10940"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG13510.1"
                     /translation="MLKKYRFLLADKLQNIVYYLIRAYSWTFRLRVENEKPWLDHLRG
                     GGRILLCCWHQQFFSAVRYFKNYAPYHPALMISQSRDGDVIARIAEKTGWHTVRGSSS
                     KDGGTALKGMIAHLSQYGFAGHILDGPRGPAGVVKAGVIRLAQVEGTFVVPIYASADR
                     AWYFRSWDRFMLPKPFARVTLRYGDMLDLASKAGEEDFERQRLRLQEIMLPGLHAGI"
BASE COUNT          358 a          413 c          452 g          330 t
ORIGIN      
        1 aaaagtgacg gaatgctatt accgggaacc ggatttgtca atgattttcg tcatgatttt
       61 ctcgggggcc ccttcatcag ccgtaagggc aagtcacgcg ttgcggaatg tcagaaaata
      121 tgcaatgttt ttgcacggca tcggcggata agctttggcg gcaggcgcgg gcggaatcca
      181 ttccgcggac cggccaacgt ccggatgaaa cagggcagca aaaggcaaag ggcagttatg
      241 gcaagtgcaa atatcctcct ggtggacgac gaagaagatt tccgctttat tttcacccgg
      301 caggtgacac gcatccttca ggacctgcaa ctggagtttt ttgaagccgg ggacggcgaa
      361 gaggcgctgg aactgctccg aacgggcgtc aagccctcca tcatcattct tgattatacg
      421 atgcccagaa tgagcggaac ggagctgctc aaaaccatcg accgcgaaca tggcgacttg
      481 gtcaacgttc cccgcctcgt cctgagcgga tatatcggtg aggacgccat cagggaagtg
      541 aagagtctgc gatgcgatta tatggaaaag agcatgaaca cgaaaatctt ttatcagcgg
      601 ttctgcaggt atctcaccga taagctgggg cttccccccg ctgaaccgga acggtaaaaa
      661 atcgcaaacg gaccggctga ttccgactca ggccgaaacg ccatcccgcg ccatgcggcg
      721 cagtcaaccg gtgccatgga acgtcatctc gcagggaaca tcctgatgct gaaaaaatac
      781 agatttttgc tggcggacaa gcttcagaat attgtttatt atctgattcg cgcctacagc
      841 tggacgttcc ggcttcgcgt ggaaaacgaa aagccctggc tcgatcacct gcggggcggc
      901 ggcaggattc ttctgtgctg ctggcatcag cagttctttt cagccgtccg ctacttcaag
      961 aattatgcgc cctatcaccc ggcgctgatg atcagccaga gcagggacgg cgacgtcatc
     1021 gcccgcattg ccgagaagac cggctggcat accgtccgcg gttcgtcgtc gaaagacggg
     1081 gggacggcgt tgaaaggcat gatcgcgcac ctgtcgcaat acggctttgc cggccatatc
     1141 ctggacggac cgagaggacc tgcgggagtg gtcaaagccg gcgtaatccg cctggcccag
     1201 gttgagggaa ctttcgtggt ccccatttac gcctccgccg accgggcctg gtatttcaga
     1261 agctgggacc gcttcatgct gcccaaaccc tttgcccgcg ttactctgcg gtatggggac
     1321 atgctggatc tcgcttcaaa ggcaggcgaa gaagattttg aacgccagag attgagactc
     1381 caggagatca tgctgccggg cctgcatgcc ggaatttgat ccgtgaaaaa cactttgcaa
     1441 aaaaccggcg gcggtcatgg gacccgccgc cggtttgggg tgaaaatcca ggggtcattt
     1501 tacggtgtct tgaagtcgaa cttgtggcac tgcgcgcagt agttttccga ttt
//
LOCUS       DAOSIM010000363         1542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_70492_length_1542_cov_9.551446, whole genome shotgun sequence.
ACCESSION   DAOSIM010000363 DAOSIM010000000
VERSION     DAOSIM010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70492_length_1542_cov_9.551446"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..>1542
                     /gene="eutB"
                     /locus_tag="PLR47_10945"
     CDS             336..>1542
                     /gene="eutB"
                     /locus_tag="PLR47_10945"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF018461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HOG13511.1"
                     /translation="MSRREFCCALAGSLAGAALLPPALRADLRTTKSGEDLFSFMNRT
                     AGGFDRTTYQQLLGAANAFKEGDRILGVAAADETARSKARLLLSNTRLGDILKHPVFE
                     DKLYSRLLESLSPDLVQKTSPMTVGEFKNFLFNAPEEKIHAILDGLCSDVIGCVVKLM
                     SNEELIAVGGKIFNPLAGTRIGARGYMGARIQPNSPTDHPEDVIWQIFSGWSYATGDV
                     VLGTNPVSSRPQSVAALEAALEDIRRTFGVENLLPHSVLAHIDLQAEVEKRQPHTTGI
                     WFQSLAGSDQANATFDITLEKMLAYASQRTGQYGFYFETGQGADFTNGSGQGTDMVIH
                     ESRKYGFARLLKIKVAEARKKAGRTPAPWVHVNDVAGFIGPEVFRTREQLVRCCLEDI
                     VMGKLHGLTI"
BASE COUNT          360 a          440 c          415 g          327 t
ORIGIN      
        1 cgattgttcc gatggtcttc ttcatgatct gtttcctccc tgagggtttt tgttttattt
       61 gccttatact taatgaaaat tttgtgccag aattggaaaa tagaagataa aagctagcaa
      121 cataaagata ttattaggct taacgtgtaa gtccaccagg ctcaacccgg cgtaaaacaa
      181 tcgtttcggc gtttttcttt caatgaaatg ttcaacggac ttgaacgatg cataaaaaag
      241 tattattcat tcccggaaaa aatttctgaa ggcagtcgaa tgatcaggct cggaccatga
      301 aggtcagtct tacaacagaa aggaaaggcg ggcgcatgag cagaagggaa ttttgttgtg
      361 cccttgccgg ttccctggcg ggcgccgcat tgctgccgcc ggcattgcgg gcggatcttc
      421 gcaccaccaa atccggcgag gatctgttct ccttcatgaa ccgcacggca ggcgggtttg
      481 accgaaccac gtatcagcag cttctcggag cggccaacgc tttcaaagag ggagaccgga
      541 ttctgggcgt ggccgcagcc gatgaaaccg cccgttccaa agcgcgcctc cttctgtcca
      601 atacgcggct gggcgacatc ctgaagcatc ccgtgtttga ggacaagctc tattcccgac
      661 tgctggaaag cctctcgcct gacctcgttc aaaaaaccag cccgatgact gtgggagaat
      721 tcaaaaattt tctgtttaac gccccggaag aaaaaatcca cgccatcctc gacggtctgt
      781 gcagcgatgt gattggctgc gtcgtaaaac tgatgtccaa tgaagagctc attgccgtgg
      841 gaggaaaaat cttcaatccg ctcgccggaa cccgaatcgg cgccaggggc tacatgggcg
      901 cccgcattca gcccaattca cccaccgacc accccgagga tgtcatctgg cagatttttt
      961 ccggctggtc ctatgcgacc ggcgatgtgg ttctcgggac caacccggtc agcagccggc
     1021 cccaatccgt cgcggcgctg gaagcggcgc tcgaagacat ccgccgcact ttcggggtgg
     1081 aaaaccttct ccctcacagt gttctggccc atattgacct tcaggcggaa gtggaaaaac
     1141 gtcaaccgca cacgaccggc atctggtttc agagccttgc gggcagcgac caggccaacg
     1201 ccacgttcga catcacgctg gagaaaatgc tggcctacgc gtcgcagcgg acgggccaat
     1261 acggatttta tttcgagacc ggccagggcg ccgacttcac caacggcagc ggccagggaa
     1321 ccgacatggt cattcacgaa tcgcgcaagt acggctttgc ccggctgctg aagatcaaag
     1381 ttgccgaagc ccggaaaaag gcgggacgca cccctgcccc gtgggttcac gtcaacgacg
     1441 tggccgggtt catcggcccg gaagttttcc gcacccgcga acagctcgtg cgctgctgcc
     1501 tggaagacat tgtcatgggc aagctgcacg gcctcaccat cg
//
LOCUS       DAOSIM010000364         1542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_70495_length_1542_cov_6.155346, whole genome shotgun sequence.
ACCESSION   DAOSIM010000364 DAOSIM010000000
VERSION     DAOSIM010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70495_length_1542_cov_6.155346"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PLR47_10950"
     CDS             complement(<1..769)
                     /locus_tag="PLR47_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOG13512.1"
                     /translation="MSAVMPEFKTLLFEEKEPHIGVVTMNRPGQLNAINLDMLNDFER
                     LFQTLYGEDSIRVLVITGAGKGYSSGADLNDAVIYKESEAFADPEKFLKLVQERYAAL
                     ILGMRRIPQPIISAVNGPAAGGGFSMTLASDIRVACREAYFVASFINIGLSGGELGCS
                     FFLPRLVGLAHASDILFTGRKVGAEEAERIGLVNKLAPREELLEAALSYARPMVGKSV
                     GGLKLTKRVLNENIHASSLETAVNLENRNQTIMVFSGE"
     gene            complement(766..>1542)
                     /locus_tag="PLR47_10955"
     CDS             complement(766..>1542)
                     /locus_tag="PLR47_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG13513.1"
                     /translation="PTIYTALLGNQKFRTMDLSFVKGYFGGAAPLPENTLKQLKDLHG
                     AIIYDVYGATENTAFATTTPWGGKVKVGTVGLPLPNTDIKIVDVDKGVNELPIGEPGE
                     ICIKGPQVMTGYYEKPEETANALRDGWFYTGDIGFFDEDGYLTISDRKKDVIVASGFN
                     VFPKEIDEILFEHPKISEACCIGIPDEYRGETVKAYIVVQPGETLTIPEVIAFCKERM
                     TAYKVPTEVEFIDELPKSAIGKIMRRELREMDRKKREKAS"
BASE COUNT          317 a          455 c          398 g          372 t
ORIGIN      
        1 actcgcctga aaaaaccatg atggtctgat tccggttttc aaggttcacg gccgtctcca
       61 gcgaggaagc atggatgttt tcattcagaa cccgcttcgt cagcttcaag ccccccacgc
      121 ttttgcccac catgggtctg gcgtaagaaa gcgccgcttc cagaagctcc tcccgcgggg
      181 ccagtttgtt gacgaggcct attctttcgg cttcctccgc gccgaccttg cggccggtga
      241 acagaatatc ggaagcgtgc gcaagcccca ccagccgggg cagaaaaaag gagcaaccca
      301 gttcgccgcc ggaaagcccg atgttgatga aagaggccac aaaataggct tccctgcacg
      361 ccacccggat atcgctggcc agcgtcatgg aaaatcctcc tccggcggcc gggccattca
      421 cggccgatat gatcggctgg ggaattcttc tcatgcccag aatcagtgcc gcgtagcgct
      481 cctgcaccag cttcaggaat ttttcaggat ccgcaaacgc ttcggactcc ttgtagatca
      541 ccgcatcatt gagatcggcg cccgaagaat accctttgcc ggcgcccgtg atgacgagaa
      601 cccggatgga atcttccccg tacagggtct gaaacagcct ttcaaagtcg ttgagcatgt
      661 ccagattgat ggcattgagc tgcccgggac ggttcatcgt cacaacgccg atgtgcggtt
      721 ctttttcttc aaagagcaac gtcttgaatt ccggcatgac cgcgctcatg aggctttctc
      781 cctttttttc ctgtccatct ccctcaattc gcggcgcatg atcttgccga tcgcgctctt
      841 gggaagctca tcgatgaatt ccacctccgt gggaacctta taggcggtca ttctctcttt
      901 gcagaaggct atcacctccg gtatcgtcag cgtctctccc ggctgaacga cgatgtaggc
      961 tttcacggtt tcacccctgt actcgtcggg aatcccaatg cagcaggcct cgctgatctt
     1021 cgggtgctcg aagagaattt catcgatctc cttgggaaaa acattgaagc cgctcgcgac
     1081 gatgacgtcc tttttgcggt ctgatatggt caggtagcca tcctcgtcga agaacccgat
     1141 atctccggta taaaaccatc catcccggag ggcattggcc gtttcttccg gtttttcata
     1201 gtacccggtc atgacctggg gacctttgat gcagatttcg ccgggctcgc cgatagggag
     1261 ttcatttacg cctttatcaa catccacgat tttaatatcc gtgttgggaa gcggaagtcc
     1321 cacggtcccg acttttacct ttccgcccca gggggtggtg gtggcaaatg ccgtattctc
     1381 ggtagcgccg tagacatcgt agatgatggc tccgtggaga tccttcaact gcttcagcgt
     1441 gttttccggc aggggcgccg ccccgccgaa atatcccttg acaaaggaaa gatccatcgt
     1501 ccggaatttc tgattgccca gaagcgccgt ataaatcgtg gg
//
LOCUS       DAOSIM010000365         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_70837_length_1537_cov_7.958165, whole genome shotgun sequence.
ACCESSION   DAOSIM010000365 DAOSIM010000000
VERSION     DAOSIM010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70837_length_1537_cov_7.958165"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..>1537
                     /locus_tag="PLR47_10960"
     CDS             107..>1537
                     /locus_tag="PLR47_10960"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOG13514.1"
                     /translation="MMKVLLAGCNVDYDLIRELHEKSGLKQDITPETISAAYARISRS
                     PRSVDQLRVDARAEVEKARRSNRNIVFEMGHSSVAEHAVFNIDVIGVSRLLVEEIEKF
                     RLCSYTEKSQRYVLFDRDFVVPEEIVRAGMREEFVETIRLQNDFYHDLYERLRPHVFE
                     KNPELAANPANRSLLEGWAKEDARYAIALATQTQLGMTVNARNLELMLRRLASLPLAE
                     ARLFSEKLYHATREIAPSLIRYTQATDYDRLTRPRLRTMAGILLEKRQVKADREKFTD
                     VRLVSATPGADRQAAAAVLFASSSQSYGRCQRRAARLSPREMKGLFQTVFENMQLYDA
                     ALRELENVDLRYELTVSASCFAQLKRHRMATIIAQDYAPRLGVTVPPSVRLVGRQKEF
                     MALMRQTEKRYDRILEKAPQAAAYLLTNAHRRRVLMKFNARELYHLSRLRADAHAQWD
                     IRNLSESMLRQARRLMPLTLMMACGKD"
BASE COUNT          318 a          477 c          455 g          287 t
ORIGIN      
        1 cgccggagta tcttttttgc gtcagcgatg tgccgtaccg ttttcagacc atcggcgaac
       61 gctttctcgg ccggacgctg ccgcgcattg agatggtgag gctttgatga tgaaggtgct
      121 cctggccggc tgcaacgtcg attacgattt gatccgtgag cttcacgaga agagcgggct
      181 gaagcaggac attacgccgg aaacgatttc cgccgcctac gcccgcatca gccgcagccc
      241 caggtccgtg gatcaactgc gtgtagacgc cagggccgaa gtcgaaaaag cccgccgatc
      301 caaccgcaac atcgttttcg agatggggca cagctcggtg gccgaacacg cggtcttcaa
      361 catcgacgtg atcggcgttt cccgcctgct ggttgaagaa atcgaaaaat tccgcctttg
      421 ctcctatacg gaaaaatccc agcggtacgt attgtttgac cgggacttcg tcgtgcctga
      481 agaaatcgtc cgggcgggaa tgcgggagga atttgtcgaa acgatccggc tgcaaaacga
      541 tttttaccat gatctctatg agcggctccg gcctcatgtc tttgagaaaa atccggaact
      601 ggccgcaaac cccgccaacc gctctttgct ggagggctgg gccaaggaag acgcgcgcta
      661 cgccatcgcg cttgccaccc agacgcaact ggggatgacc gtcaacgccc gcaatctgga
      721 gctgatgctg cgccggctgg cctccctgcc gctggcggag gcgcgccttt tcagcgagaa
      781 gctctatcac gcaacccggg aaatcgcgcc gtcgctgatt cgctacaccc aagctacaga
      841 ttacgaccgc ctgacccggc cgcggctgcg gaccatggcc ggaatccttt tggagaagcg
      901 gcaagtgaaa gcagaccggg aaaagttcac cgatgtccgg ctcgtatccg cgacgcccgg
      961 cgcggaccgg caggccgccg ccgcggtttt gtttgcttct tcctctcaga gctacggacg
     1021 ttgccagcgc cgcgccgccc ggctgagccc ccgcgagatg aaaggcttgt tccaaaccgt
     1081 gtttgaaaac atgcagctct acgatgccgc gctaagggaa ctggaaaacg tcgatctgcg
     1141 ctacgagctg acggtaagcg cgagctgctt tgcccagctc aagcgccacc gcatggcgac
     1201 catcatcgcc caggactacg cccccaggct gggcgtgacc gttccgccct ccgtgcgcct
     1261 agtcggccgg caaaaggaat tcatggccct catgcggcag acggaaaaga gatatgatcg
     1321 cattctggaa aaagcgccgc aggcggcggc ttatcttctg accaacgcgc accgcagaag
     1381 ggtacttatg aaattcaacg cccgggagct ttaccatctg tcccgcctgc gggcggacgc
     1441 gcacgcccag tgggatatcc ggaatctgtc ggaaagcatg cttcggcagg cgcgccgcct
     1501 catgccgctg acgctgatga tggcctgcgg caaggac
//
LOCUS       DAOSIM010000366         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_72234_length_1517_cov_6.135431, whole genome shotgun sequence.
ACCESSION   DAOSIM010000366 DAOSIM010000000
VERSION     DAOSIM010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72234_length_1517_cov_6.135431"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..459)
                     /locus_tag="PLR47_10965"
     CDS             complement(4..459)
                     /locus_tag="PLR47_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13515.1"
                     /translation="MLASLKLLDVMANNSEAIARNWAKDVAQNAKTPSYHDLHENHLI
                     PQAADFYRKLSSVFTLKDPYPAIYDFMSKFAEKRFREEVPLQEVLYAIILMRRHIWLY
                     AEFQAIFVTITEQQQALESQTRTILIFDYIAYILTDKYMALMGAAPKKK"
     gene            complement(865..>1517)
                     /locus_tag="PLR47_10970"
     CDS             complement(865..>1517)
                     /locus_tag="PLR47_10970"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG13516.1"
                     /translation="VPLAHRLILLHTFMIRTEVVDEEVITSRLDRGEKGIVAIWHQRM
                     YGVIGYAKAASRYRPSAIISRSGDGDLISELVRRLHFRPIRGSSSRGGKEALAAIVED
                     LKENLLAIHAVDGPRGPGGTVKAGLIRMAELSGAKIFPVYVSFSRAWPLRSWDRFLIP
                     KPFSRVRIRWDQPISVPANMKEQEFESIRRSLEIHMRQNQITDDRSFGQESLLLEP"
BASE COUNT          348 a          396 c          432 g          341 t
ORIGIN      
        1 cgtttatttt ttcttgggag cggctcccat taatgccatg tatttatccg tcaggatata
       61 agcgatgtaa tcgaaaataa gaatcgtccg cgtctgactt tccagggcct gctgctgctc
      121 ggtaatggta acgaagatgg cctgaaactc ggcgtaaagc caaatgtggc ggcgcatgag
      181 aatgatggcg taaagcacct cctgcagcgg aacttcttcc ctgaaacgtt tttcggcaaa
      241 tttggacatg aagtcgtaaa tggcggggta aggatctttc agggtaaaga cactgcttaa
      301 cttgcggtaa aaatccgcag cctgaggaat aagatgattt tcatggagat cgtgatagga
      361 aggcgtcttg gcgttttggg cgacgtcttt cgcccagttt cgtgcaatcg cctcggaatt
      421 gtttgccatc acatccagta gcttcaacga tgccagcata tgccttctcc ctgcttcttt
      481 gggaaacctg ccggtttccc catgaggatg ctgcatgact gcggatcaaa gagcggactg
      541 cctcggtttc ccccaaccaa tgagcaatcg gcgtgccaga aatgtcattt tacgcaaacc
      601 attgattttg ctgatggtgc gaagaccatg ccattgaacg ccgtcggaac gggcgacccg
      661 aatgtcttcg gtttgatcgc gcaccgtcaa gggcacaccc ggaaaatgca gaccggtgca
      721 ggttttgagc aggatgtctt atttgtcacg ggggcgcgac gtaaatgtcg cgttcgggaa
      781 aatccgcggc ctcatgattc cgtctgtgag gaaaaattgc gaaaagcaga tcaacgaaaa
      841 ggacggcagc cccgtgaaac aaacctatgg ttcaaggagc agactctctt gaccgaaaga
      901 ccggtcgtcg gtgatctggt tttgccgcat gtgaatttcc agcgaccggc ggatggattc
      961 gaactcctgt tccttcatgt ttgccggaac ggagatgggc tggtcccagc ggatccggac
     1021 ccggctgaaa ggtttgggaa tcagaaaccg gtcccagctt cgcaaaggcc aggctcggct
     1081 gaaggagacg taaaccggaa agattttcgc gccggaaagc tcggccatgc ggatcaatcc
     1141 ggctttgacc gtgccgccgg gtccacgggg cccatccacc gcatggatag cgagaagatt
     1201 ttctttcagg tcctccacga tggcggcgag cgcctctttc cctcccctgg aactggagcc
     1261 ccgaatgggg cggaaatgga ggcgacggac cagctcgctg atcagatcgc cgtcgccgct
     1321 tcggctgatg atggccgaag gccggtatcg gctagcggcc ttggcatagc cgatgactcc
     1381 gtacatgcgc tgatgccaga tcgccacaat tcccttttct ccccggtcca gacgggaggt
     1441 aatcacctct tcatcgacca cctccgtgcg aatcataaag gtgtgaagca ggatcaggcg
     1501 gtgagccagg ggcacaa
//
LOCUS       DAOSIM010000367         1516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_72300_length_1516_cov_7.711157, whole genome shotgun sequence.
ACCESSION   DAOSIM010000367 DAOSIM010000000
VERSION     DAOSIM010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72300_length_1516_cov_7.711157"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PLR47_10975"
     CDS             complement(<1..279)
                     /locus_tag="PLR47_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOG13517.1"
                     /translation="MTVWWIFFAVFILAMLALDLGVFNRKAHVIQMREAMLWTAFWVS
                     LALCFGAAIYFLYGHGKAIEYVTAYLIEYSLSIDNLFVFMLIFRYFDVP"
     gene            complement(298..1494)
                     /locus_tag="PLR47_10980"
     CDS             complement(298..1494)
                     /locus_tag="PLR47_10980"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG13518.1"
                     /translation="MPYYIGDVIQDQNKLIARTPEKFRESGIDVQISARVEGIDPARG
                     EVSLQDGRIFPYDVLVMATGTSAFVPDMPGLDLPGVVSLRSLEDAVRIKTWLREKNAK
                     RVVTIGGGFIGLEMSEALSAAGISTTIIHKDPLPANRWDPVLSQMMLEELQAQGVKFV
                     GGASAQGVEKGTSAPLRVLTDRGDYEGDLVLLAIGVRPDANLARATGLKIGTTGAIAV
                     NFSQQTSIENIYAAGDCCESFHRVSRRWVNVPLGDIANKQGRVAGRNIGGRPMTFDGI
                     VGAQSFRLFSLQCAAAGLSEREAIAAGFSPASHVAWGSAMAPALGMRKIGLKLTADRS
                     TGRLLGAQAVGAAGAVGRINALSVALWAGMDLDQVGYLDLAYSPPFSPAWDIIHNAAQ
                     ALGRSL"
BASE COUNT          321 a          475 c          447 g          273 t
ORIGIN      
        1 gggaacgtcg aagtagcgaa agatcagcat gaagacaaag agattgtcga tgctcagcga
       61 gtattcgatc aggtaagcgg tcacatactc gatggccttt ccgtggccgt acagaaagta
      121 gattgccgct ccgaaacaca gggccagcga gacccagaag gccgtccaga gcatggcttc
      181 cctcatttgg atgacgtggg ctttccggtt gaagacgccc agatcgagcg ccagcatcgc
      241 cagaatgaac accgcaaaga agatccacca aacggtcatg acatccgatt ctcccgctta
      301 caatgaacgg ccgagcgcct gggccgcgtt atggatgata tcccaggccg ggctgaaagg
      361 cggcgagtag gccaggtcca ggtagcctac ctgatccaga tccatacccg cccagagtgc
      421 gacggaaagc gcgttgattc tgcctaccgc accggcggcc cccaccgcct gcgcgcccag
      481 cagtctgccc gtcgatcggt cggccgtcag cttgaggccg atttttctca tccccagcgc
      541 gggcgccatc gcgcttcccc aggcgacatg acttgccgga gagaagccgg cggcgatggc
      601 ttccctttcc gacagccccg cggccgcgca ttgaagcgaa aacagacgga aggactgggc
      661 gccgacgatg ccgtcaaacg tcatgggccg gccgccgatg ttgcgacccg caacgcggcc
      721 ctgcttgttg gcgatgtcgc ccaggggaac attcacccag cggcggctga cgcgatgaaa
      781 agattcgcag cagtcgcccg ccgcataaat gttttcgatg gaggtctgct gggaaaaatt
      841 gacggcaatc gcgccggtgg taccgatttt caaacccgtg gcccgcgcca ggttcgcatc
      901 aggccgaacg ccgatggcca gaagcaccag atcgccttcg taatctcccc ggtccgtcag
      961 aacgcgcagc ggcgcgctgg ttcctttttc aacgccttga gcggacgcgc ctccgacaaa
     1021 tttcacacct tgcgcctgaa gctcttcgag catcatttgc gacaggaccg gatcccagcg
     1081 gttggccgga agcggatcct tgtgaatgat ggttgtggaa atgcccgcgg ccgagagcgc
     1141 ctcgctcatt tccaggccga tgaagccgcc gccgattgtg acgacccgct tcgcattttt
     1201 ttcccgaagc caggtcttga tgcggacggc gtcctccagg ctgcgcaggg aaaccacgcc
     1261 cggcaggtca agccccggca tatccggcac aaacgcggag gtgccggtgg ccatcaccag
     1321 aacgtcatag gggaaaatcc gcccgtcctg aagcgaaact tcacccctgg ccggatcgat
     1381 gccttccacc ctggccgaaa tctgaacgtc aatgccgctc tcgcggaatt tttccggcgt
     1441 gcgggcgatc agtttgtttt gatcctgaat tacatccccg atgtaatagg gcatgggtca
     1501 ggcggcaaag gcgaca
//
LOCUS       DAOSIM010000368         1509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_72835_length_1509_cov_4.917469, whole genome shotgun sequence.
ACCESSION   DAOSIM010000368 DAOSIM010000000
VERSION     DAOSIM010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72835_length_1509_cov_4.917469"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PLR47_10985"
     CDS             complement(<1..763)
                     /locus_tag="PLR47_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG13519.1"
                     /translation="MNRVYERLRDRLETMASGYPATQNGVELKILQKLFSEEDAALFL
                     QMSPEPDTAQELALRLGAEVADTAARLEDMARRGLIFRIKAGEGIRYRPVPFIVGIYE
                     YQLNHLNLPLLKDISKYYLTGLGATFHGLKTPHLRSIPINTEIVADRPVFPYDDAASI
                     LRSKSRIAVAECFCRKAVRLYGKTCVHPAETCLQFDAFADYYVENGMARYIDTDEALA
                     ILRQSEAEGLVVHVLNSREVEAMCCCCSCCCGMLIS"
     gene            1221..>1509
                     /locus_tag="PLR47_10990"
     CDS             1221..>1509
                     /locus_tag="PLR47_10990"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG13520.1"
                     /translation="MKKIFFVICIFLLSVIFSFSAFSQSSNPLLDEGIKQYQAEDLEE
                     AIETLKKARAADPTSSMAAFVLGMAYKRTNDLPDAEKTLRDAVSLTPKIKEA"
BASE COUNT          368 a          392 c          362 g          387 t
ORIGIN      
        1 gcgaaatcag catgccgcaa cagcaggaac agcagcagca cattgcttcc acctcccggg
       61 aattcaggac atggaccacc aatccttccg cctcgctctg ccttaggatc gccagcgcct
      121 cgtcggtgtc gatgtagcgc gccatgccat tttccacgta gtagtctgca aaggcgtcaa
      181 actgaaggca ggtttccgcg ggatgaacgc aggttttccc gtacaggcgg acggcctttc
      241 ggcagaagca ttcggccacg gcaatccgcg atttgctcct gaggatggac gccgcgtcgt
      301 cgtaagggaa aaccggccgg tcggcgacga tttcggtgtt gatcggaatg gaacgcagat
      361 ggggcgtttt cagaccgtga aacgtggccc ccaatccggt aaggtaatac tttgaaatgt
      421 ctttcagcag cggcaaattc aaatgattca gctggtattc gtaaatgccg acgatgaacg
      481 gaaccggacg atagcggatc ccctcgccgg ctttgatccg gaaaatcagc ccccgacggg
      541 ccatgtcttc aagccttgcc gccgtgtccg caacctcggc acccagacgc agagccagtt
      601 cctgtgccgt atccggttcg gggctcattt gcagaaacag cgccgcatcc tcttcggaaa
      661 aaagcttttg aagaatttta agttcgacgc cgttttgtgt cgcgggatag ccgctcgcca
      721 tcgtctcgag cctgtcgcgc agcctttcat agacacggtt cattttctga ctaacctctc
      781 aggttgttag ggtattcgcc tgcgtgacga ataagaatag ttttgcaata ctctcctgcg
      841 gccaccgcca tgataatcct tgccattgcc ttcaaaattt ctcctttttt gccctgtatt
      901 gatggatcaa aaaagtttct gggcggccat cgtttttata gtgtaacccg accttcattg
      961 caatcataaa ataggttatc ttcggttcat tgaagcattc gtgatcaaga aaaggggacg
     1021 gccaaagaaa gaatcagtcg atgagtaaat taaatagaac cgtccccttt tttccaaata
     1081 gaaccgtccc cttttttcca cttttttcca catcttcaac accaaaaata tcttgctttt
     1141 ggatatgctt aggatttatg attctacgca cagaaaatat gtggaagtgg attctaaact
     1201 catttacgcg gagggctttg atgaaaaaaa tattttttgt catctgcatt tttttactgt
     1261 ccgtcatctt ttccttttcg gctttttccc agagcagcaa tccccttctg gatgaaggaa
     1321 tcaagcagta tcaggcggag gatttggaag aagccattga gaccttaaaa aaggccagag
     1381 ccgcggaccc gacatcgtcc atggcggctt tcgttctggg catggcttat aagcggacaa
     1441 atgatttgcc ggacgctgaa aaaactctgc gggacgcagt ttccctcacg cccaaaatca
     1501 aagaagcct
//
LOCUS       DAOSIM010000369         1509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_1701
            NODE_72841_length_1509_cov_3.640990, whole genome shotgun sequence.
ACCESSION   DAOSIM010000369 DAOSIM010000000
VERSION     DAOSIM010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863230
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,200
            CDSs (total)                      :: 2,190
            Genes (coding)                    :: 2,181
            CDSs (with protein)               :: 2,181
            Genes (RNA)                       :: 10
            tRNAs                             :: 9
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72841_length_1509_cov_3.640990"
                     /isolate="AMR_MDS_1701"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PLR47_10995"
     CDS             <1..683
                     /locus_tag="PLR47_10995"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOG13521.1"
                     /translation="VEKQKDGYHLIRPRDKRKDQTYFLYHIDAKILSRVLFPLGPYTK
                     EEVRALADRADLPVAAKAESQDVCFVTQRTYQEFLRERLGEQKNGRIVDQEGHGLGSH
                     KGIAYYTVGQRGGLGISHPVPLYVLRIDAKENEIVVGEKKALAAKELIAGDLNLFRSD
                     WPEMVKAKIRYRKKESPCRVSFENGRAWVVFMEDQEAITPGQSVVFYEGDNVLGGGVI
                     EEVVHGAA"
     gene            670..>1509
                     /gene="nadA"
                     /locus_tag="PLR47_11000"
     CDS             670..>1509
                     /gene="nadA"
                     /locus_tag="PLR47_11000"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOG13522.1"
                     /translation="MELLEKIAQLKRERNAVILVHNYQLPEVQDIGDFVGDSLGLSME
                     AAKTNADVIVFCGVYFMAETAKILSPEKTVLIPDPKAGCPMADMITADELKALKAKHP
                     GAAVLCYVNTTAEVKAECDLCCTSANTVRMVQEALADRDEIIFVPDKHLGDFVIEKTG
                     RKMILWQGFCPTHAKILPDDIIEQKRLHPGAAVVSHPECPRAVRELSDQVLSTEGMCR
                     FVKQSDVQEIIVATEIGIIHRMQKENPGNIFYPVSKHAVCPNMKRINLEKVLWALEDM
                     DHAV"
BASE COUNT          368 a          340 c          463 g          338 t
ORIGIN      
        1 ccgttgaaaa gcaaaaagac gggtatcatc tgatccgacc ccgtgacaaa aggaaagatc
       61 agacttattt tctttaccat attgacgcga aaattctgtc gcgggttctt ttcccccttg
      121 gtccctacac caaggaagag gtgagagctc tggccgacag ggcagatctc ccggtggcgg
      181 ccaaggcgga aagtcaggat gtgtgttttg tgacccagag aacctatcag gagtttctgc
      241 gggaacggct gggtgagcaa aaaaacggac gcattgtcga tcaggaagga cacggtttgg
      301 ggagccataa ggggatcgct tactatactg tggggcagcg gggcggactt ggcatcagtc
      361 atccggtgcc cttgtatgta ctcaggattg atgcgaaaga gaatgagatt gttgtggggg
      421 agaaaaaagc gcttgcggca aaagaactga tcgccggaga tttgaacctg ttccggtcgg
      481 attggccgga gatggttaaa gcgaagattc gctaccgcaa aaaagaaagc ccctgtcggg
      541 tgagttttga gaatggtcgt gcgtgggtgg tctttatgga ggatcaggaa gccatcacgc
      601 cgggtcagtc ggtggttttt tacgagggag acaatgtcct ggggggcggc gttattgagg
      661 aggttgtcca tggagctgct tgaaaagatt gcacaattga agcgggaacg caatgccgtc
      721 atcctggtcc acaattacca gttgccggaa gtgcaggaca tcggggattt cgtgggggat
      781 tccctggggc tgagtatgga agcagccaaa accaacgccg atgttatcgt tttctgcggt
      841 gtttacttca tggccgagac ggcaaaaatt ctgtcaccgg aaaaaacggt cctcattcct
      901 gatcccaagg cgggatgtcc catggcggac atgattaccg ccgacgaact gaaggcgctg
      961 aaagcgaaac atcccggtgc ggcggtgctt tgttatgtca atacaacggc ggaggttaag
     1021 gccgaatgtg atctctgctg cacatcggcc aacactgtgc ggatggttca ggaagctctg
     1081 gccgaccggg atgaaatcat ttttgttccc gacaagcatc tgggcgattt cgtgattgaa
     1141 aagaccggga ggaaaatgat cctctggcag ggtttctgcc cgacgcatgc gaagattctg
     1201 ccggatgata tcatcgaaca gaagcggctc catcctggtg cggcagtcgt gtcccatccc
     1261 gaatgccccc gtgcggtgcg ggagctttcc gatcaggtcc tgtccacgga ggggatgtgc
     1321 cgctttgtga agcaaagcga tgtacaggaa atcattgtgg caacggagat cggcatcatc
     1381 catcggatgc agaaagaaaa cccgggcaat atcttttatc ctgtttccaa acacgccgtt
     1441 tgcccgaaca tgaagcggat caatctggaa aaggtgctct gggccctgga ggatatggat
     1501 catgctgtt
//
